Technetium-99m-labeled proteins for imaging inflammatory foci.
Polyclonal human IgG (IgG), antinuclear antibody (TNT-1), and human serum albumin (HSA), were labeled with 99mTc by a method recently developed in our laboratory, and administered i.v., each to a separate group of five mice, bearing inflammatory foci induced by an i.m. injection of 40 microL turpentine or 5 x 10(8) E. coli and 5 x 10(8) Entercocci. TNT-1 labeled with 125I served as a control and 67Ga-citrate as a "gold standard". At 4 or 24 h post injection, animals were imaged and sacrificed for tissue distribution studies. At 4 h in the turpentine group, the abscess-to-muscle ratios were: 67Ga, 4.8 +/- 2.1, 125I-TNT-1, 4.3 +/- 1; 99mTc-TNT-1, 3.5 +/- 1.8; 99mTc-IgG, 3.9 +/- 0.6; and 99mTc-HSA, 4.3 +/- 1. In the microorganism group, these ratios were 2.6 +/- 0.6, 3.3 +/- 0.5, 3.4 +/- 0.08, 3 +/- 1.1 and 4.1 +/- 0.6, respectively. Autoradiographic examination of infected tissues indicated that leakage of labeled proteins into interstitial space due to increased capillary permeability may be one of the major mechanisms of uptake.
Thakur
M L
ML
Thomas Jefferson University, Philadelphia, PA 19107.
DeFulvio
J
J
Park
C H
CH
Damjanov
A
A
Yaghsezian
H
H
Jungkind
D
D
Epstein
A
A
McAfee
J G
JG
D000818:Animals; D000974:Antibodies, Antinuclear; D001345:Autoradiography; D005260:Female; D005710:Gallium Radioisotopes; D007074:Immunoglobulin G; D007249:Inflammation / Q000139:chemically induced / Q000000981:diagnostic imaging*; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D008297:Male; D051379:Mice; D011506:Proteins; D011786:Quality Control; D011877:Radionuclide Imaging; D013667:Technetium; D014018:Tissue Distribution
D000974:Antibodies, Antinuclear; D005710:Gallium Radioisotopes; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D011506:Proteins; D013667:Technetium