Uncoupling thermotolerance from the induction of heat shock proteins.
Exposure of cells to elevated temperatures causes a rapid increase in the synthesis of heat shock proteins (hsps) and induces thermotolerance, the increased ability of cells to survive exposure to lethal temperatures; however, the connection between hsp induction and the acquisition of thermotolerance is unclear. hsp induction in the yeast Saccharomyces cerevisiae is mediated by the activation of heat-shock transcription factor, and recently we have described a mutation, hsf1-m3, in heat-shock transcription factor that prevents the factor's activation. We now demonstrate that this mutation results in a general block in heat-shock induction but does not affect the acquisition of thermotolerance. Our results indicate that high-level induction of the major hsps is not required for cells to acquire thermotolerance.
Smith
B J
BJ
University of California, San Diego, Department of Biology, La Jolla 92093.
Yaffe
M P
MP
D001483:Base Sequence; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D005800:Genes, Fungal; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009154:Mutation; D009838:Oligodeoxyribonucleotides; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D009838:Oligodeoxyribonucleotides; D001616:beta-Galactosidase; D019204:GTP-Binding Proteins