Lysis and fractionation of Mycobacterium paratuberculosis and Escherichia coli by matrix solid-phase dispersion.
A novel method for the lysis and subsequent fractionation of bacterial constituents from Mycobacterium paratuberculosis strain 19698 (M. paratuberculosis) and Escherichia coli strain DH5 alpha utilizing the technique of matrix solid-phase dispersion (MSPD) is described. Bacteria were blended with octadecylsilyl (C18) derivatized silica to obtain cellular lysis. The blended material was used to prepare a column which was sequentially eluted with solvents of increasing polarity. Fractionation of cellular components was confirmed by analysis of the solvent extracts. The possible applicability of the MSPD technique as a general method for the lysis and fractionation of bacterial components is proposed.
Hines
M E
ME
Department of Veterinary Pathology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803.
Long
A R
AR
Snider
T G
TG
Barker
S A
SA
D001431:Bacteriological Techniques; D001433:Bacteriolysis; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D008855:Microscopy, Electron, Scanning; D009161:Mycobacterium / Q000737:chemistry*; D010283:Paratuberculosis / Q000382:microbiology