Circadian patterns of melatonin, corticosterone, and progesterone in male rats subjected to chronic stress: effect of constant illumination.
Plasma and pineal melatonin and plasma corticosterone and progesterone concentrations have been shown to be altered by several types of stressors. This study was designed to define the circadian patterns of the hormones mentioned above in rats subjected to chronic stress and to investigate the influence of constant illumination. The results revealed that melatonin and corticosterone circadian patterns deteriorated and their plasma concentrations were significantly elevated. The constant illumination (2,500 lux) during the dark period (from 2000 to 0600) was not able to suppress melatonin production in stressed animals, while the plasma content of corticosterone was decreased at the end of experimental period compared to control rats. Plasma levels of progesterone were increased in stressed animals as well. Constant illumination, however, provoked also an increase of progesterone secretion in controls. Statistical comparisons between hormonal secretory patterns showed that melatonin and corticosterone correlated negatively in controls (r = -0.58, P less than 0.05) during the nighttime. However, in stressed animals correlation was observed only between melatonin and progesterone secretion during the light and dark period (r = -0.43, P less than 0.05). Surprisingly, the correlation during the nighttime in rats subjected to constant illumination was negative (r = -0.60, P less than 0.02) compared to positive correlation (r = 0.60, P less than 0.02) in rats kept under normal lighting regimen. These results suggest that melatonin release is affected by stress and, possibly, under these circumstances, interacts with adrenal steroid secretion.
Persengiev
S
S
Department of Immunoneuroendocrinology, Bulgarian Academy of Sciences, Sofia.
Kanchev
L
L
Vezenkova
G
G
D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology*; D003345:Corticosterone / Q000097:blood*; D008029:Lighting; D008297:Male; D008550:Melatonin / Q000097:blood*; D009043:Motor Activity / Q000502:physiology; D010870:Pineal Gland / Q000378:metabolism; D011374:Progesterone / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains; D012892:Sleep Deprivation / Q000502:physiology*; D013223:Statistics as Topic; D013312:Stress, Physiological / Q000150:complications*
D011374:Progesterone; D008550:Melatonin; D003345:Corticosterone