Anatomical mapping of choline acetyltransferase (ChAT)-like and glutamate decarboxylase (GAD)-like immunoreactivity in outer hair cell efferents in adult rats.
The distribution of choline acetyltransferase (ChAT)-like and glutamate decarboxylase (GAD)-like immunoreactivity in the cochleae of 15 adult Wistar white rats was investigated using the peroxidase-antiperoxidase (PAP) technique. A monoclonal antibody to ChAT and a polyclonal antiserum to GAD were used. Immunoreaction was investigated quantitatively, in the electron microscope, on tangential sections of the tunnel of Corti and the rows of outer hair cells. ChAT-like and GAD-like immunoreactivity was found in all efferent nerve fibres in the tunnel of Corti and in all efferent synapses on the outer hair cells. A coexistence of ChAT and GAD in the efferent system to the outer hair cells of the rat is therefore assumed.
Dannhof
B J
BJ
Zoologisches Institut der J.W. Goethe-Universität, Frankfurt am Main, Federal Republic of Germany.
Roth
B
B
Bruns
V
V
D000367:Age Factors; D000818:Animals; D001489:Basilar Membrane / Q000201:enzymology; D002795:Choline O-Acetyltransferase / Q000032:analysis*; D003051:Cochlea / Q000201:enzymology* / Q000294:innervation / Q000648:ultrastructure; D004525:Efferent Pathways / Q000201:enzymology* / Q000648:ultrastructure; D005968:Glutamate Decarboxylase / Q000032:analysis*; D007124:Immunoenzyme Techniques; D051381:Rats; D011919:Rats, Inbred Strains
D002795:Choline O-Acetyltransferase; D005968:Glutamate Decarboxylase