title: Evaluation of the new improved BHI-lysis blood culture medium for the BCB Roche system.
abstract: The new BHI-lysis blood culture medium for the BCB system (BCB release, Hoffmann-La Roche), which lyses blood cells, was compared with the BHI-S broth of the same BCB system and the Signal system (Oxoid). A total of 2394 sets consisting of three bottles were each inoculated with 7 ml of blood at the bedside. In the laboratory agar-coated paddles were attached to the BHI-lysis and the BHI-S bottles, and the Signal device was mounted onto the Oxoid bottle. All systems were incubated at 35 degrees C for seven days. Of the 309 (13%) positive sets, 73 (3%) were contaminated and in 242 (10%) sets a total of 250 pathogenic microorganisms were isolated. These could be grown significantly more often from the BHI-lysis bottle (n = 213) than from either the BHI-S (n = 189) or Signal bottle (n = 176). No significant differences in the time needed to achieve a positive result was noted between the BHI-lysis and BHI-S bottles, but comparison of the BHI-lysis and Signal bottles revealed that overall pathogens were detected earlier significantly more often with the BHI-lysis bottle. In view of the good performance of blood culture broths containing lysing agents, their wider use is warranted in future.
authors.affiliation: Laboratoire Central de Bactériologie, Hôpital Cantonal Universitaire de Genève, Switzerland.
authors.forename: P
authors.initials: P
authors.lastname: Rohner
authors.forename: H
authors.initials: H
authors.lastname: Burkardt
authors.forename: H
authors.initials: H
authors.lastname: Dreismann
authors.forename: R
authors.initials: R
authors.lastname: Auckenthaler
mesh_terms: D000328:Adult; D001419:Bacteria / Q000302:isolation & purification; D001769:Blood / Q000382:microbiology*; D003470:Culture Media; D005069:Evaluation Studies as Topic; D006801:Humans; D008828:Microbiological Techniques; D009996:Osmotic Fragility; D013211:Staphylococcus aureus / Q000302:isolation & purification; D015003:Yeasts / Q000302:isolation & purification
chemical_list: D003470:Culture Media