title: How does the mRNA pass through the ribosome?
abstract: A working model of the mRNA path through the ribosome is proposed. According to the model, the template goes around the small ribosomal subunit along the region where its 'head' is separated from other parts of the subunit. The 5'-end of the mRNA fragment covered by the ribosome is located near the 3'-terminus of 16S rRNA, whereas the 3'-terminal residues of the fragment are situated on the outer surface of the subunit, opposite its 'side ledge'. When associated with the 50S subunit, the 30S subunit is oriented in such a manner that the decoding center faces the L7/L12 stalk. Implications of the proposed working model of the mRNA topography for the function of the ribosome are discussed.
authors.affiliation: AN Belozersky Laboratory, Moscow State University, USSR.
authors.forename: I N
authors.initials: IN
authors.lastname: Shatsky
authors.forename: A V
authors.initials: AV
authors.lastname: Bakin
authors.forename: A A
authors.initials: AA
authors.lastname: Bogdanov
authors.forename: V D
authors.initials: VD
authors.lastname: Vasiliev
mesh_terms: D001665:Binding Sites; D008854:Microscopy, Electron; D008954:Models, Biological; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D012333:RNA, Messenger; D012336:RNA, Ribosomal, 16S; D012343:RNA, Transfer