HML-1 antigen on mucosa-associated T cells, activated cells, and hairy leukemic cells is a new integrin containing the beta 7 subunit.
The monoclonal antibodies HML-1, B-ly7 and Ber-ACT8 recognize intramucosal gut T lymphocytes, activated cells, and hairy cell leukemia. The antigen on hairy cells consists of three glycoproteins (160 kappa D, 130 kappa D and 105 kappa D unreduced; 145 kappa D and 120 kappa D reduced). These peptides have biochemical features reminiscent of integrins but we have shown by immunoprecipitation that they are not known integrin subunits. We have used a newly produced antibody (BP6) to purify this molecule and shown by N-terminal sequence analysis that the smallest subunit is the product of integrin beta 7 cDNA. This molecule is thus a new member of the integrin family of leucocyte adhesion proteins. Immunoprecipitation experiments indicate that the two larger subunits are recognized by HML-1, B-ly7 and Ber-ACT8.
Micklem
K J
KJ
Leukaemia Research Fund Immunodiagnostics Unit, Nuffield Department of Pathology, John Radcliffe Hospital, Headington, Oxford, United Kingdom.
Dong
Y
Y
Willis
A
A
Pulford
K A
KA
Visser
L
L
Dürkop
H
H
Poppema
S
S
Stein
H
H
Mason
D Y
DY
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000737:chemistry; D014408:Biomarkers, Tumor; D002846:Chromatography, Affinity; D006801:Humans; D039641:Integrin beta Chains; D016023:Integrins / Q000737:chemistry / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007943:Leukemia, Hairy Cell / Q000378:metabolism* / Q000473:pathology; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D013601:T-Lymphocytes / Q000378:metabolism* / Q000502:physiology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D039641:Integrin beta Chains; D016023:Integrins; C070709:integrin alphaEbeta7; C091368:integrin beta7