A new peptide analog (RM06) modulates the growth of hematopoietic cells.
The bombesin carboxy terminal dipeptide Leu-Met, was bound to hypoxanthine with a pentoxy-carbonyl chain. The new purine derivative, N-omega-[5(1,6-dihydro-6-oxo-9-purinyl)] pentoxy-carbonyl-L-leucil-L- methionine-COOH (RM06), significantly augmented human bone-marrow derived granulocyte-monocyte colony forming units (CFU-GM) when added to the cell-culture medium. Moreover, in vivo hemopoietic reconstitution was more rapid in lethally irradiated bone-marrow reconstituted BDF-1 mice, treated with RM06. Both in vitro and in vivo effects were similar to those exerted by recombinant granulocyte-monocyte colony stimulating factor (GM-CSF). It is concluded that the new modified dipeptide modulates the growth of both human and murine hematopoietic cells in vitro and in vivo.
Cornaglia-Ferraris
P
P
Pediatric Oncology Research Laboratory, Gaslini Children Hospital, Genoa, Italy.
Biano
A
A
Corrias
M V
MV
Stradi
R
R
D000595:Amino Acid Sequence; D000818:Animals; D016026:Bone Marrow Transplantation / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D003114:Colony-Forming Units Assay; D004151:Dipeptides / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007042:Hypoxanthines / Q000494:pharmacology*; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry
D004151:Dipeptides; D007042:Hypoxanthines; D010455:Peptides; C063739:RM 06; D016178:Granulocyte-Macrophage Colony-Stimulating Factor