A single lymphocyte culture for fragile X induction and prometaphase chromosome analysis.
A single lymphocyte culture system is described which produces both reliable fragile X expression and elongated chromosomes for prometaphase analysis. This system, which is based on that described by Wheater and Roberts in 1987, involves the deoxycytidine release of a thymidine block. Eight fragile X positive subjects had an average expression level of 26%, with a range of 12% to 45%, using the thymidine/deoxycytidine protocol. This was comparable to the levels obtained in parallel cultures treated with thymidine alone or fluorodeoxyuridine.
Griffiths
M J
MJ
Regional Cytogenetics Unit, Birmingham Maternity Hospital, Edgbaston.
Strachan
M C
MC
D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D002873:Chromosome Fragility; D003841:Deoxycytidine / Q000494:pharmacology; D005260:Female; D005600:Fragile X Syndrome / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008677:Metaphase; D008875:Middle Aged; D013936:Thymidine / Q000494:pharmacology; D014960:X Chromosome
D003841:Deoxycytidine; D013936:Thymidine