Identification of a novel serum amyloid A protein in BALB/c mice.
Four serum amyloid A protein (SAA) genes and two SAA gene products, SAA1 and SAA2, were identified in BALB/c mice. Using analytical isoelectric focusing we have identified a quantitatively significant new member of the SAA family and designated it 'SAA5'. This protein has characteristics never before described for any SAA molecule. In the highly conserved region between amino acids 33 and 44, identical in all SAAs from all species examined, SAA5 had four amino acid substitutions. In addition, the induction of SAA5 by lipopolysaccharide had different kinetics from that of the other mouse SAAs. Our data suggest that the mouse SAA gene family is more complex in composition and regulation than previously surmised.
de Beer
M C
MC
Division of Rheumatology, University of Kentucky College of Medicine, Lexington.
Beach
C M
CM
Shedlofsky
S I
SI
de Beer
F C
FC
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases; D005260:Female; D015151:Immunoblotting; D007525:Isoelectric Focusing; D008075:Lipoproteins, HDL / Q000097:blood / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000302:isolation & purification; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid; D000685:Serum Amyloid A Protein / Q000032:analysis* / Q000235:genetics
D008075:Lipoproteins, HDL; D010446:Peptide Fragments; D000685:Serum Amyloid A Protein; D010450:Endopeptidases