A monoclonal antibody which recognizes the inner and outer segments of the photoreceptor cells in the vertebrate retina.
A monoclonal antibody (MAb-1E7), generated against bovine retinal homogenate, labeled the outer and inner segment layers of the vertebrate retina. Immuno-electron microscopic observation clearly demonstrated that antigen(s) bound by MAb-1E7 was localized in the cell membrane of the outer segment and the distal portion of the inner segment. Western blot analysis revealed that MAb-1E7 recognized 40 kD- and 27 kD-polypeptides. Mouse retina with hereditary photoreceptor degeneration (C3H/He and CBA strains) did not involve the MAb-1E7 immunoreactive structures. The present immunocytochemical observation demonstrated that MAb-1E7 was highly specific to the outer segment of the photoreceptor cells and, therefore, can be a useful marker for the cells.
Ono
K
K
Third Department of Anatomy, Okayama University Medical School, Japan.
Tokunaga
A
A
Shibata
N
N
Tanaka
H
H
Kurose
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D051379:Mice; D008815:Mice, Inbred Strains; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D010786:Photoreceptor Cells / Q000276:immunology*; D011784:Quail; D051381:Rats; D012160:Retina / Q000276:immunology*
D000911:Antibodies, Monoclonal