title: Induction of arthritis in rats by soluble peptidoglycan-polysaccharide complexes produced by human intestinal flora.
abstract: Peptidoglycan-polysaccharide complexes were isolated from feces of a healthy subject and from ileostomy fluid from 5 patients. Peptidoglycan-polysaccharide complexes were tested for arthritogenicity in a rat model, by subcutaneous injection in Freund's incomplete adjuvant. Complexes from the healthy subject did not induce arthritis, but those from ileostomy fluid of 1 of the patients induced severe, chronic joint inflammation. We concluded that peptidoglycan-polysaccharide complexes from intestinal flora are potentially arthritogenic in rats. This arthritogenicity may be influenced by the content of muramic acid and rhamnose in these complexes.
authors.affiliation: Department of Immunology, Erasmus University, Rotterdam, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Kool
authors.forename: J G
authors.initials: JG
authors.lastname: Ruseler-van Embden
authors.forename: L M
authors.initials: LM
authors.lastname: van Lieshout
authors.forename: H
authors.initials: H
authors.lastname: de Visser
authors.forename: M Y
authors.initials: MY
authors.lastname: Gerrits-Boeye
authors.forename: W B
authors.initials: WB
authors.lastname: van den Berg
authors.forename: M P
authors.initials: MP
authors.lastname: Hazenberg
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001168:Arthritis / Q000139:chemically induced*; D001644:Bifidobacterium / Q000378:metabolism*; D005051:Eubacterium / Q000378:metabolism*; D005260:Female; D006801:Humans; D007422:Intestines / Q000382:microbiology; D008875:Middle Aged; D009112:Muramic Acids / Q000032:analysis; D010457:Peptidoglycan / Q000009:adverse effects* / Q000032:analysis / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D012210:Rhamnose / Q000032:analysis
chemical_list: D009112:Muramic Acids; D010457:Peptidoglycan; D012210:Rhamnose