Rps4 maps near the inactivation center on the mouse X chromosome.
RPS4Y, a Y-linked gene in humans, appears to encode an isoform of ribosomal protein S4. A homologous locus on the human X chromosome, RPS4X, lies close to the X-inactivation center but fails to undergo X-inactivation. We have isolated a genomic clone from the mouse Rps4 locus, the homolog of human RPS4X. We derived an intron probe that hybridizes to the functional Rps4 locus but does not cross-hybridize to related sequences elsewhere in the mouse genome. Genetic mapping utilizing interspecific mouse backcrosses and the intron-specific probe demonstrates that Rps4 maps close to the Phka locus on the mouse X chromosome and in the vicinity of the X-inactivation center. The gene order Ccg-1-Rps4/Phka-Xist-Pgk-1 is conserved between mouse and human.
Hamvas
R M
RM
Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, London, United Kingdom.
Zinn
A
A
Keer
J T
JT
Fisher
E M
EM
Beer-Romero
P
P
Brown
S D
SD
Page
D C
DC
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D015342:DNA Probes; D004303:Dosage Compensation, Genetic; D005260:Female; D006801:Humans; D007438:Introns; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D012269:Ribosomal Proteins / Q000235:genetics*; D013045:Species Specificity; D014960:X Chromosome
D015342:DNA Probes; D012269:Ribosomal Proteins; C031798:ribosomal protein S4