Affinity electrophoresis used for determination of binding constants for antibody-antigen reactions.
The use of affinity electrophoresis in agarose gels for determination of binding constants for the interaction of antigens with monoclonal antibodies is exemplified for monoclonal anti-human serum albumin and anti-alpha 1-fetoprotein antibodies. The calculated binding constants are verified by independent binding assays. The electrophoretic separation of antigen-antibody complexes of different stoichiometry is also demonstrated. Thus, affinity electrophoresis represents an alternative method for both qualitative and quantitative assessment of antigen-antibody interactions.
Heegaard
N H
NH
NIMH Neuroscience Center, St. Elizabeths, Laboratory of Biochemical Genetics, Washington, D.C. 20032.
Bjerrum
O J
OJ
D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D007122:Immunoelectrophoresis; D007457:Iodine Radioisotopes; D009391:Nephelometry and Turbidimetry
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes