Extended type-1 chain glycosphingolipid antigens. Isolation and characterization of trifucosyl-Leb antigen (III4V4VI2Fuc3Lc6).
A Lewis-b-active glycosphingolipid containing a repetitive type-1 chain carbohydrate core was isolated from human colonic adenocarcinoma cell line Colo205. This glycosphingolipid was purified by HPLC and preparative high-performance thin-layer chromatography and its structure elucidated by positive-ion fast-atom-bombardment mass spectrometry with collision-induced disassociation, 1H-NMR spectroscopy and methylation analysis. The glycosphingolipid was found to be a trifucosylated derivative of this novel carbohydrate core, having the following structure: [formula; see text].
Stroud
M R
MR
Biomembrane Institute, Seattle, WA 98119.
Levery
S B
SB
Salyan
M E
ME
Roberts
C E
CE
Hakomori
S
S
D000230:Adenocarcinoma; D000951:Antigens, Neoplasm / Q000276:immunology / Q000302:isolation & purification*; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003110:Colonic Neoplasms; D006028:Glycosphingolipids / Q000276:immunology* / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D007983:Lewis Blood Group Antigens; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D006028:Glycosphingolipids; D007983:Lewis Blood Group Antigens; C072748:trifucosyl-Lewis-b antigen