Affinity and conformation of the estrogen receptor when bound to single-stranded vs. double-stranded nonspecific DNA.
The affinity of the hormone-bound estrogen receptor for single-stranded and double-stranded DNA was compared using isocratic elution chromatography. The receptor bound single-stranded DNA with a two-fold higher affinity than double-stranded DNA (17.9 x 10(4) M-1 vs. 9.1 x 10(4) M-1) at 0.2 M KCl. The same number of ions were released when the receptor bound either single-stranded or double-stranded DNA (11.8 vs. 10.6, respectively). These results indicate the hormone-bound estrogen receptor has no strong preference for single-stranded vs. double-stranded nonspecific DNA, and has a similar conformation when bound to either form of DNA at physiological salt concentrations.
Skafar
D F
DF
Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201.
D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D005260:Female; D011487:Protein Conformation; D011960:Receptors, Estrogen / Q000737:chemistry / Q000378:metabolism*
D004277:DNA, Single-Stranded; D011960:Receptors, Estrogen; D004247:DNA