Elucidation of the sequence of canine (pro)-calcitonin. A molecular biological and protein chemical approach.
From the canine thyroid gland a calcitonin (CT) immunoreactive peptide was purified by successive aqueous acid acetone extraction, gel filtration and HPLC. Gas-phase sequencing of the purified peptide showed that the first 25 amino acids had 65% sequence homology with the amino-terminus of the human CT prohormone. A canine cDNA library was then made from the thyroid gland. A plasmid was isolated containing a sequence that is homologous to part of exon 3, and the complete sequence of exon 4 of the human mRNA encoding preproCT. From this cDNA the amino acid sequence of canine CT is predicted. In comparison with well-known CT sequences of other species, the strongest homology exists with bovine, porcine and ovine CT.
Mol
J A
JA
Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Kwant
M M
MM
Arnold
I C
IC
Hazewinkel
H A
HA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002116:Calcitonin / Q000032:analysis* / Q000302:isolation & purification; D004247:DNA / Q000032:analysis*; D004285:Dogs; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000032:analysis*; D016415:Sequence Alignment; D013961:Thyroid Gland / Q000737:chemistry*
D011498:Protein Precursors; D002116:Calcitonin; D004247:DNA