title: Inhibition of farnesyl transferases from malignant and non-malignant cultured human lymphocytes by prenyl substrate analogues.
abstract: Cytosolic prenyl transferases from two human lymphoid tissue-derived cell lines, IM-9 and Molt-4 cells, are shown to isoprenylate recombinant p21H-ras. Isoprenylation was inhibited by an N-acetylated pentapeptide (N-Ac-Lys-Cys-Val-Leu-Ser), c,t-farnesyl diphosphate, c,t,t-geranylgeranyl diphosphate, t,t,t-geranylgeranyl diphosphate and a photolabile farnesyl diphosphate analogue. c,t-Farnesyl and t,t,t-geranylgeranyl monophosphates were also effective inhibitors of the Molt-4 enzyme but not the IM-9 enzyme.
authors.affiliation: Department of Biochemistry and Molecular Biology, J. Hillis Miller Health Center, University of Florida, Gainesville 32610.
authors.forename: N P
authors.initials: NP
authors.lastname: Das
authors.forename: C M
authors.initials: CM
authors.lastname: Allen
mesh_terms: D019883:Alkyl and Aryl Transferases; D000595:Amino Acid Sequence; D006801:Humans; D007938:Leukemia / Q000201:enzymology*; D008969:Molecular Sequence Data; D011106:Polyisoprenyl Phosphates / Q000494:pharmacology*; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism; D012717:Sesquiterpenes; D014166:Transferases / Q000037:antagonists & inhibitors*; D014407:Tumor Cells, Cultured
chemical_list: D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; C004808:farnesyl pyrophosphate; D014166:Transferases; D019883:Alkyl and Aryl Transferases; C064911:p21(ras) farnesyl-protein transferase; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras); C002963:geranylgeranyl pyrophosphate