Molecular cloning of the Candida maltosa ADE1 gene.
The structural gene (ADE1) encoding phosphoribosyl-aminoimidazole-succinocarboxamide synthetase (SAICAR synthetase; EC 6.3.2.6) in Candida maltosa has been isolated by functional complementation of an ade1 strain of Saccharomyces cerevisiae. The gene was localized on a 2.5-kb BamHI DNA fragment. Nucleotide sequence analysis of the cloned gene has revealed an open reading frame encoding a protein (SAICAR synthetase) with an Mr of 32,751. The codon bias index, 0.68, indicates that the ADE1 gene is a moderately highly expressed gene. The cloned gene shows 63.5% nt identity and 65.2% deduced amino acid identity with the S. cerevisiae ADE1 gene which encodes the same enzymatic activity. The gene may be used as a convenient genetic marker for construction of a new host-vector system for C. maltosa.
Sasnauskas
K
K
Department of Molecular Biology, Institute of Applied Enzymology, Vilnius, Lithuanian Republic.
Jomantiene
R
R
Geneviciute
E
E
Januska
A
A
Lebedys
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D002175:Candida / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010453:Peptide Synthases / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D003062:Codon; D010453:Peptide Synthases; C022379:phosphoribosylaminoimidazole-succinocarboxamide synthetase