Uptake and metabolism of choline by rat brain after acute choline administration.
The present study is concerned with the uptake and metabolism of choline by the rat brain. Intraperitoneal administration of choline chloride (4-60 mg/kg) caused a dose-dependent elevation of the plasma choline concentration from 11.8 to up to 165.2 microM within 10 min and the reversal of the negative arteriovenous difference (AVD) of choline across the brain to positive values at plasma choline levels of greater than 23 microM. Net choline release and uptake were linearly dependent on the plasma choline level in the physiological range of 10-50 microM, whereas the CSF choline level was significantly increased only at plasma choline levels of greater than 50 microM. The bolus injection of 60 mg/kg of [3H]choline chloride caused the net uptake of greater than 500 nmol/g of choline by the brain as calculated from the AVD, which was reflected in a minor increase of free choline level and a long-lasting increase of brain phosphorylcholine content, which paralleled the uptake curve. Loss of label from phosphorylcholine 30 min to 24 h after choline administration was accompanied by an increase of label in phosphatidylcholine, an indication of a delayed transfer of newly taken-up choline into membrane choline pools. In conclusion, homeostasis of brain choline is maintained by a complex system that interrelates choline net movements into and out of the brain and choline incorporation into and release from phospholipids.
Klein
J
J
Department of Pharmacology, University of Mainz, F.R.G.
Köppen
A
A
Löffelholz
K
K
Schmitthenner
J
J
D000818:Animals; D001158:Arteries; D001921:Brain / Q000378:metabolism*; D002021:Buffers; D002555:Cerebrospinal Fluid / Q000378:metabolism; D002794:Choline / Q000097:blood / Q000378:metabolism* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006706:Homeostasis; D007700:Kinetics; D008297:Male; D009994:Osmolar Concentration; D010767:Phosphorylcholine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014680:Veins
D002021:Buffers; D010767:Phosphorylcholine; D002794:Choline