Ca2+ binding properties of type X collagen.
Type X collagen is a developmentally regulated collagen that is only synthesized by chondrocytes of the hypertrophic and calcifying zone in fetal cartilage. There is evidence in the literature that type X collagen may be involved in cartilage calcification. Here we show that type X collagen synthesis precedes calcium deposition in nodules of fetal human chondrocytes forming in cell culture and present evidence that type X collagen binds calcium in a specific and dose dependent manner. In an assay using bovine type X collagen coupled to beads and 45Ca2+ we determined a total of about 15 binding sites per alpha 1(X) chain with a dissociation of 32 microM.
Kirsch
T
T
Max-Planck-Society, Clinical Research Units for Rheumatology, University of Erlangen-Nürnberg, Germany.
von der Mark
K
K
D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D002358:Cartilage, Articular / Q000166:cytology* / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D003094:Collagen / Q000302:isolation & purification / Q000378:metabolism*; D005333:Fetus; D006132:Growth Plate; D006801:Humans; D007700:Kinetics
D003094:Collagen; D002118:Calcium