title: A plant homologue to mammalian brain 14-3-3 protein and protein kinase C inhibitor.
abstract: We have isolated cDNA clones of Spinacea oleracea L. and Oenothera hookeri of 930 and 1017 base pairs, respectively. The open reading frame deduced from the Oenothera sequence codes for a protein of a calculated molecular mass of 29,200. The primary amino acid sequence exhibits a very high degree (88%) of homology to the 14-3-3 protein from bovine brain, and protein kinase C inhibitor from sheep brain. Subsequently the plant protein was partially purified from leaf extract. The partially purified plant protein inhibited protein kinase C from sheep brain in a heterologous assay system. The active fraction consisted of 5-6 different polypeptides of similar molecular size. One of these proteins crossreacted with a peptide-specific antibody against protein kinase C inhibitor protein from sheep brain.
authors.affiliation: Botanisches Institut, Universität Kiel, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Hirsch
authors.forename: A
authors.initials: A
authors.lastname: Aitken
authors.forename: U
authors.initials: U
authors.lastname: Bertsch
authors.forename: J
authors.initials: J
authors.lastname: Soll
mesh_terms: D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000276:immunology; D010940:Plant Proteins / Q000235:genetics* / Q000276:immunology; D010944:Plants / Q000235:genetics* / Q000276:immunology; D012689:Sequence Homology, Nucleic Acid; D014446:Tyrosine 3-Monooxygenase
chemical_list: D048948:14-3-3 Proteins; D009419:Nerve Tissue Proteins; D010940:Plant Proteins; D014446:Tyrosine 3-Monooxygenase