Uptake, distribution and binding of vertebrate and invertebrate steroid hormones and time-dependence of ponasterone A binding in Calliphora vicina. Comparisons among cholesterol, corticosterone, cortisol, dexamethasone, 5 alpha-dihydrotestosterone, 1,25-dihydroxyvitamin D3, ecdysone, estradiol-17 beta, ponasterone A, progesterone, and testosterone.
The presence of specific binding sites for radiolabelled vertebrate-type and arthropod-type steroid hormones was investigated in several organs including salivary gland, and central nervous system of third instar Calliphora vicina larvae by thaw-mount autoradiography. Ponasterone A, a 20-hydroxyecdysone agonist and 20-hydroxyecdysone are the only steroids which bind to nuclear high affinity binding sites. These binding sites are DNA associated while nucleoli show no tracer binding. Ecdysone, an endogenous 20-hydroxyecdysone precursor, is taken up by target cells but no significant nuclear binding occurs. 1,25-Dihydroxyvitamin D3 concentrates in cytoplasm only and its uptake is highest compared to all other steroids. Progesterone and testosterone show weak accumulation in the cytoplasm, while for cholesterol, corticosterone, cortisol, dexamethasone, dihydrotestosterone and estradiol-17 beta, no noticeable uptake occurs. For ponasterone A, a clear time dependence of uptake and intracellular distribution is visible, suggesting the existence and involvement of specific ecdysteroid uptake and transport mechanisms. These results suggest the presence of binding sites for various mammalian steroids in insects. Whether vertebrate steroid hormones or metabolites of them play a role in insects or whether the uptake and binding is based on chemical similarities alone without biological significance remains to be further investigated.
Bidmon
H J
HJ
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599-7090.
Stumpf
W E
WE
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites / Q000502:physiology; D002117:Calcitriol / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D003345:Corticosterone / Q000378:metabolism; D003907:Dexamethasone / Q000378:metabolism; D004175:Diptera / Q000378:metabolism*; D004440:Ecdysone / Q000378:metabolism*; D004441:Ecdysterone / Q000031:analogs & derivatives* / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D006728:Hormones / Q000378:metabolism*; D006854:Hydrocortisone / Q000378:metabolism; D007700:Kinetics; D009474:Neurons / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism; D012469:Salivary Glands / Q000378:metabolism; D013256:Steroids / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism
D006728:Hormones; D013256:Steroids; D004440:Ecdysone; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D004441:Ecdysterone; D003907:Dexamethasone; C008999:ponasterone A; D002784:Cholesterol; D002117:Calcitriol; D003345:Corticosterone; D006854:Hydrocortisone