title: In vivo study of the state of order of the membranes of gram-negative bacteria by Fourier-transform infrared spectroscopy (FT-IR).
abstract: Temperature-induced order/disorder transition profiles were obtained from the membranes of intact Gram-negative bacterial cells by FT-IR analysis of the frequency shifts of the acyl chain methylene symmetric stretching band as a monitor. Cells grown at different temperatures yielded distinct transition profiles. At the individual growth temperatures, however, the nearly alike frequency values indicated a very similar 'state of order' of the bacterial membranes. The FT-IR data were complemented by GC analysis of whole cell fatty acid composition. The FT-IR data obtained in vivo gave direct evidence of the adaptation of the 'state of order' and 'fluidity' of bacterial membranes to varying growth temperatures.
authors.affiliation: Department of Cytology, Robert Koch-Institute, Berlin, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Schultz
authors.forename: D
authors.initials: D
authors.lastname: Naumann
mesh_terms: D002462:Cell Membrane / Q000502:physiology*; D005583:Fourier Analysis; D006090:Gram-Negative Bacteria / Q000502:physiology*; D008563:Membrane Lipids / Q000032:analysis; D013055:Spectrophotometry, Infrared / Q000379:methods; D013816:Thermodynamics
chemical_list: D008563:Membrane Lipids