title: Isolation and primary structure of a novel pheromonotropic neuropeptide structurally related to leucopyrokinin from the armyworm larvae, Pseudaletia separata.
abstract: A novel pheromonotropic neuropeptide has been isolated from a head extract of the armyworm larvae, Pseudaletia separata, by a seven step purification procedure using an in vivo assay with decapitated female moths of Bombyx mori. Amino acid sequence analysis and comparison with synthetic peptides established the primary structure of the peptide, termed Pseudaletia pheromonotropin (Pss PT), as H-Lys-Leu-Ser-Tyr-Asp-Asp-Lys-Val-Phe-Glu-Asn-Val-Glu-Phe-Thr-Pro-Arg-Le u-NH2. Pss PT is structurally related to leucopyrokinin, an insect myotropic neuropeptide, and possesses the C-terminal pentapeptide, Phe-Thr-Pro-Arg-Leu-NH2, responsible for the biological activity.
authors.affiliation: Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Matsumoto
authors.forename: A
authors.initials: A
authors.lastname: Fónagy
authors.forename: M
authors.initials: M
authors.lastname: Kurihara
authors.forename: K
authors.initials: K
authors.lastname: Uchiumi
authors.forename: T
authors.initials: T
authors.lastname: Nagamine
authors.forename: M
authors.initials: M
authors.lastname: Chijimatsu
authors.forename: T
authors.initials: T
authors.lastname: Mitsui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D005612:Freeze Drying; D007814:Larva; D007915:Lepidoptera / Q000235:genetics*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics* / Q000302:isolation & purification; D009842:Oligopeptides / Q000235:genetics*; D010675:Pheromones / Q000235:genetics*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D012689:Sequence Homology, Nucleic Acid
chemical_list: D009479:Neuropeptides; D009842:Oligopeptides; D010675:Pheromones; C049557:leucopyrokinin; C072549:pheromonotropin, Pseudaletia; D011761:Pyrrolidonecarboxylic Acid