Application of a monoclonal antibody-based enzyme-linked immunosorbent assay for detection of an inflammatory response antigen in subclinical mastitic milk samples.
A monoclonal antibody to a 23.5-kDa bovine inflammatory antigen present in high levels in mastitic milk has been used in an antigen-capture enzyme-linked immunosorbent assay (ELISA) to screen milk samples from herds of cattle for subclinical mastitis. The results from 20 herds with a total of 2,612 quarter samples are presented. Good correlation was observed between the ELISA level and the milk cell count (MCC). The results demonstrated an average of 5% false negatives (1.8% associated with isolates of Staphylococcus aureus and/or Streptococcus spp.) and 7.7% false positives for each herd in relation to mastitic (greater than 400,000 cells per ml) or nonmastitic (less than 400,000 cells per ml) MCCs.
Ball
H J
HJ
Veterinary Research Laboratories, Stormont, Belfast, United Kingdom.
Finlay
D
D
Mackie
D P
DP
Greer
D
D
Pollock
D
D
McNair
J
J
D000118:Acetylglucosaminidase / Q000096:biosynthesis; D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D002452:Cell Count; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D005260:Female; D008414:Mastitis, Bovine / Q000175:diagnosis* / Q000276:immunology; D008892:Milk / Q000166:cytology / Q000276:immunology*; D012044:Regression Analysis
D000911:Antibodies, Monoclonal; D000118:Acetylglucosaminidase