Generation of T cell clones binding F(ab')2 fragments of the idiotypic immunoglobulin in patients with monoclonal gammopathy.
Lymphocytes from two patients with multiple myeloma stage I and one patient with monoclonal gammopathy of undetermined significance were found to proliferate specifically in response to low concentrations of F(ab')2 fragments of the autologous M component. T cell clones isolated from repeatedly stimulated cultures bound specifically the autologous idiotype and proliferated after addition of soluble idiotype and exogenous interleukin-2. The majority of clones were CD8+ and showed negligible staining for CD4. Idiotype-binding clones could not be isolated from cultures of lymphocytes from a healthy control stimulated under the same conditions. The study provides support for the existence of idiotype-reactive T cells in monoclonal gammopathies. Such cells might have a regulatory role on the tumor cell clone and may be important for a future therapeutic approach.
Osterborg
A
A
Department of Oncology (Radiumhemmet), Karolinska Hospital, Stockholm, Sweden.
Masucci
M
M
Bergenbrant
S
S
Holm
G
G
Lefvert
A K
AK
Mellstedt
H
H
D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology* / Q000378:metabolism; D007130:Immunoglobulin Idiotypes / Q000276:immunology* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D008213:Lymphocyte Activation / Q000502:physiology; D009101:Multiple Myeloma / Q000097:blood / Q000276:immunology; D010265:Paraproteinemias / Q000097:blood / Q000276:immunology*; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D007140:Immunoglobulin Fab Fragments; D007130:Immunoglobulin Idiotypes