[Expression of human tissue plasminogen activator by recombinant cell lines from various animals].
A number of recombinant cell lines which produce human tissue plasminogen activator (tPA) was obtained using different hosts--mouse, rat, hamster, simian and human cell lines. All types of recombinant lines secreted active r-tPA into conditioned medium. A slight difference between molecular weights of secreted variants of r-tPA was mediated by the different mechanisms of protein modification. Treatment of some recombinant cell lines with different substances resulted in increased levels of r-tPA production.
Aleshkov
S B
SB
Ustav
M B
MB
Baev
A A
AA
D016475:3T3 Cells; D000818:Animals; D015139:Blotting, Southern; D015153:Blotting, Western; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D006031:Glycosylation; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D010957:Plasmids; D051381:Rats; D011995:Recombination, Genetic; D010959:Tissue Plasminogen Activator / Q000235:genetics* / Q000378:metabolism
D004247:DNA; D010959:Tissue Plasminogen Activator
Ekspressiia tkanevogo aktivatora plazminogena cheloveka rekombinantnymi liniiami kletok razlichnykh zhivotnykh.