title: Up-modulation of phorbol dibutyrate receptors by carbachol and arachidonic acid in rat prostatic epithelial cells.
abstract: Phorbol dibutyrate (PDBu) binding to rat prostatic epithelial cells has been measured as an indirect determination of protein kinase C in this cell system. Analysis of [3H]PDBu binding using competitive displacement demonstrated a single class of PDBu receptors with a Kd = 141 nM and a binding capacity of 4.8 pmol PDBu bound/mg cell protein. Raising cytosolic Ca2+ levels by redistribution of intracellular Ca2+ after cell treatment with carbachol or arachidonic acid (which also affects the bulk biophysical properties of the cell membrane) resulted in up-regulation of the available number of PDBu receptors. These results appear to be a consequence of PKC translocation from the cytosolic compartment to the plasma membrane after a cytosolic Ca2+ increase, confirming previous results in other cell systems.
authors.affiliation: Departamento de Bioquimica y Biología Molecular, Universidad de Alcalá, Madrid, Spain.
authors.forename: M P
authors.initials: MP
authors.lastname: García-Paramio
authors.forename: M J
authors.initials: MJ
authors.lastname: Carmena
authors.forename: J C
authors.initials: JC
authors.lastname: Prieto
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology; D001667:Binding, Competitive; D029742:Caenorhabditis elegans Proteins; D002118:Calcium / Q000378:metabolism; D002217:Carbachol / Q000494:pharmacology; D002352:Carrier Proteins; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D008297:Male; D015240:Phorbol 12,13-Dibutyrate / Q000378:metabolism*; D011467:Prostate / Q000166:cytology / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011955:Receptors, Drug / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D001095:Arachidonic Acids; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D011955:Receptors, Drug; C083141:phorbol ester binding protein; C027776:phorbol ester receptor; D015240:Phorbol 12,13-Dibutyrate; D002217:Carbachol; D011493:Protein Kinase C; D002118:Calcium