[The production of specific immunoenzyme conjugates to human immunoglobulins by the glutaraldehyde method].
The results of studies aimed at obtaining class-specific conjugates to human immunoglobulins to be used in the enzyme immunoassay (EIA) are presented. At the first stage of the studies purified IgA, IgM and IgG preparations were obtained. These preparations were used for obtaining immunologically active immunosorbents on the basis of bromocyanic Sepharose. Specific antibodies to human IgA, IgM and IgG were isolated from animal sera by the method of affinity chromatography. These antibodies were conjugated with peroxidase by the glutaraldehyde method. The specific activity of the conjugates were determined in EIA. The results thus obtained revealed that all preparations exhibited high specific activity and gave no cross reactions with immunoglobulins of other classes.
Shkurina
E A
EA
Semenova
G B
GB
Iankina
N F
NF
D000918:Antibody Specificity; D002846:Chromatography, Affinity; D003429:Cross Reactions; D005976:Glutaral; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000097:blood / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000097:blood / Q000302:isolation & purification; D007136:Immunoglobulins / Q000097:blood / Q000302:isolation & purification*; D007163:Immunosorbent Techniques; D007202:Indicators and Reagents; D009101:Multiple Myeloma / Q000276:immunology; D008258:Waldenstrom Macroglobulinemia / Q000276:immunology
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D007202:Indicators and Reagents; D005976:Glutaral
Poluchenie spetsificheskikh immunofermentnykh kon''iugatov k immunoglobulinam cheloveka glutaral'degidnym metodom.