title: Phospholipids and protein kinase C in acetylcholine-dependent signal transduction in Ascaris suum.
abstract: Quantitatively, the major phospholipid in the muscle of the nematode Ascaris suum was found to be phosphatidylcholine (lecithin). Stimulation of Ascaris muscle with acetylcholine or the agonists carbachol and levamisole increased the level of phosphorylcholine, 1,2-diacylglycerides and phosphatidic acid. Increased levels of these compounds, together with the demonstration of phospholipase C activity, suggest that phospholipid hydrolysis may be associated with the ACh response of the muscle via second messenger pathways. In other tissues, diacylglycerides and phosphatidic acid have been reported to regulate protein kinase C activity. Protein kinase C activity also was demonstrated in the muscle of Ascaris. For optimal activity the kinase was dependent upon Ca2+, unsaturated 1,2-diacylglyceride and phospholipid. All of the data are in accord with the possible involvement of a second messenger system being operative in the ACh-stimulated contraction of Ascaris muscle.
authors.affiliation: Department of Biological Sciences, University of Notre Dame, IN 46556.
authors.forename: J
authors.initials: J
authors.lastname: Arevalo
authors.forename: H J
authors.initials: HJ
authors.lastname: Saz
mesh_terms: D000109:Acetylcholine / Q000502:physiology*; D000818:Animals; D001200:Ascaris / Q000187:drug effects / Q000201:enzymology*; D002217:Carbachol / Q000494:pharmacology; D002794:Choline / Q000032:analysis; D005260:Female; D007978:Levamisole / Q000494:pharmacology; D009132:Muscles / Q000201:enzymology / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D010743:Phospholipids; D007978:Levamisole; D002217:Carbachol; D011493:Protein Kinase C; D010740:Phospholipases; D002794:Choline; D000109:Acetylcholine