Localization of the gene encoding the alpha 2 subunit of the human VLA-2 receptor to chromosome 5q23-31.
The alpha 2 subunit of the VLA-2 receptor (CD49B) was mapped to human chromosome 5 by several independent approaches. First, the expression of the alpha 2 subunit at the protein level was investigated in a panel of human-mouse hybrid cell lines. Cell surface expression was detected by indirect immunofluorescence with monoclonal anti-alpha 2 antibody 12F1. Intracellular alpha 2 antigen was detected by immunostaining of whole cell extracts or of immunoprecipitated 12F1 antigen with the monoclonal antibodies 3H8 and 5C5. Second, the presence of human genomic alpha 2 sequences in the panel of human-mouse hybrids was detected by PCR, using primers derived from the published alpha 2 cDNA sequence. The specificity of the amplification product was shown by direct sequencing. The results of the PCR study were confirmed by amplifying a CD14 gene fragment, known to map to chromosome 5. Finally, in situ hybridization with a 3H-labeled 1040-bp cDNA probe, also obtained by PCR, confirmed and refined the localization of CD49B on chromosome 5 at q23-31.
Jaspers
M
M
Center for Human Genetics, University of Leuven, Belgium.
Marynen
P
P
Aly
M S
MS
Cuppens
H
H
Hilliker
C
C
Cassiman
J J
JJ
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016029:Receptors, Very Late Antigen / Q000235:genetics*
D016029:Receptors, Very Late Antigen