title: Low-resolution genome map of the malaria mosquito Anopheles gambiae.
abstract: We have microdissected divisions of the Anopheles gambiae polytene chromosomes, digested the DNAs with a restriction enzyme, and PCR-amplified the DNA fragments to generate a set of pooled probes, each corresponding to approximately 2% of the mosquito genome. These divisional probes were shown to have high complexity. Except for those derived from near the centromeres, they hybridize specifically with their chromosomal sites of origin. Thus, they can be used to map cloned DNAs by a dot blot procedure, which is much more convenient than in situ hybridization to polytene chromosomes. We discuss additional potential uses of these probes, such as easier isolation of molecular markers and genes, including those that cross-hybridize with clones available from other insects. It is expected that the probes will substantially accelerate molecular genetic analysis of this most important malaria vector.
authors.affiliation: Biological Laboratories, Harvard University, Cambridge, MA 02138.
authors.forename: L
authors.initials: L
authors.lastname: Zheng
authors.forename: R D
authors.initials: RD
authors.lastname: Saunders
authors.forename: D
authors.initials: D
authors.lastname: Fortini
authors.forename: A
authors.initials: A
authors.lastname: della Torre
authors.forename: M
authors.initials: M
authors.lastname: Coluzzi
authors.forename: D M
authors.initials: DM
authors.lastname: Glover
authors.forename: F C
authors.initials: FC
authors.lastname: Kafatos
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics*; D002874:Chromosome Mapping; D002875:Chromosomes / Q000502:physiology / Q000648:ultrastructure; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D016678:Genome; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA