title: The effect of albumin or crystalloid resuscitation on bacterial translocation and endotoxin absorption following experimental burn injury.
abstract: Burn injury induces immune suppression and increases susceptibility to infection. Hypoalbuminemia is an early and consistent finding following thermal injury and is independently associated with gastrointestinal dysfunction and increased rates of infectious morbidity. This study assessed the effects of albumin resuscitation on burn-induced immunosuppression, bacterial translocation, and absorption of gut endotoxin. Male Sprague-Dawley rats, presensitized to keyhole limpet hemocyanin (KLH), underwent a 20% dorsal scald burn injury, followed by laparotomy and IVC catheterization for fluid resuscitation. Animals were randomized to one of three resuscitative regimens: Ringer's lactate 3 ml/kg/% burn, Ringer's lactate 9 ml/kg/% burn, or 5% human albumin 3 ml/kg/% burn. Delayed hypersensitivity (DTH) responses to KLH were depressed 24 hr following injury (preburn 8.9 +/- 0.2 mm, post-burn 3.1 +/- 0.3 mm, P less than 0.001) and were significantly lower in animals in whom gram-negative bacterial translocation had occurred (2.3 +/- 0.4 vs 3.6 +/- 0.2 mm, P less than 0.005). Serum albumin levels were lower and rates of gram-negative bacterial translocation higher for those animals receiving low volume crystalloid resuscitation; animals resuscitated with albumin or high volume crystalloid experienced similar degrees of postinjury hypoalbuminemia and bacterial translocation. Uptake of radiolabeled endotoxin was maximal in animals resuscitated with albumin. Bacterial translocation is believed to be responsible for a significant number of late nosocomial infections following trauma. These data suggest that the adequacy of early resuscitation rather than the type of resuscitative solution is the more important factor in minimizing translocation.
authors.affiliation: Department of Surgery, Dalhousie University, Halifax, N.S. Canada.
authors.forename: R
authors.initials: R
authors.lastname: O'Brien
authors.forename: J
authors.initials: J
authors.lastname: Murdoch
authors.forename: R
authors.initials: R
authors.lastname: Kuehn
authors.forename: J C
authors.initials: JC
authors.lastname: Marshall
mesh_terms: D000818:Animals; D002056:Burns / Q000276:immunology*; D004731:Endotoxins / Q000493:pharmacokinetics*; D006090:Gram-Negative Bacteria / Q000502:physiology; D006968:Hypersensitivity, Delayed; D007408:Intestinal Absorption; D007422:Intestines / Q000382:microbiology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012151:Resuscitation; D012709:Serum Albumin / Q000032:analysis*
chemical_list: D004731:Endotoxins; D012709:Serum Albumin