Daunorubicin impairs 1-beta-D-arabinofuranosylcytosine uptake.
Daunorubicin (DNR) and 1-beta-D-arabinofuranosylcytosine (Ara-C) act in a schedule-dependent fashion on leukemic cells. The present study was undertaken to determine whether the inhibition exerted by DNR on Ara-C cytotoxicity on HL 60 cells could be accounted for by an impairment of its uptake. After 250 ng/ml DNR treatment for 30 min, the time course of Ara-C uptake was measured, up to 128 min of incubation at 37 degrees C and at 4 degrees C, compared with controls. We found that DNR inhibits Ara-C uptake both at 37 and 4 degrees C, mostly acting on passive diffusion.
Dal Pozzo
O
O
UO Ematologia, Università degli Studi-USL 10/D, Firenze, Italia.
Bernabei
P A
PA
Lombardini
L
L
Fenigli
S
S
D'Ippolito
G
G
Rossi Ferrini
P
P
D003561:Cytarabine / Q000008:administration & dosage / Q000378:metabolism*; D003630:Daunorubicin / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D007951:Leukemia, Myeloid / Q000188:drug therapy / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism
D003561:Cytarabine; D003630:Daunorubicin