Crystallization and preliminary X-ray analysis of the lactose-specific phosphocarrier protein IIAlac of the phosphoenolpyruvate: sugar phosphotransferase system from Staphylococcus aureus.
The IIA constituent of the lactose permease from Staphylococcus aureus has been crystallized in two different forms. Crystals of form I have been grown from polyethylene glycol 4000 with beta-octyl glucoside. They diffract to 3.0 A resolution and belong to space group C2 with unit cell dimensions a = 141.7 A, b = 130.7 A, c = 96.5 A and beta = 96.2 degrees. Form II crystals have been obtained from a solution containing polyethylene glycol 400, ammonium sulfate and manganese chloride. They diffract to at least 2.8 A resolution and belong to space group P2(1)2(1)2(1) with unit cell dimensions a = 89.9 A, b = 101.5 A and c = 90.9 A.
Celikel
R
R
Department of Biology, University of California, San Diego, La Jolla 92093-0319.
Dai
X P
XP
Stewart
G C
GC
Sutrina
S L
SL
Saier
M H
MH
Xuong
N H
NH
Reizer
J
J
D029968:Escherichia coli Proteins; D007202:Indicators and Reagents; D026901:Membrane Transport Proteins / Q000737:chemistry*; D009004:Monosaccharide Transport Proteins; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000737:chemistry* / Q000302:isolation & purification; D010750:Phosphoproteins / Q000737:chemistry* / Q000302:isolation & purification; D011092:Polyethylene Glycols; D011487:Protein Conformation; D013211:Staphylococcus aureus / Q000201:enzymology*; D027981:Symporters; D014961:X-Ray Diffraction
D029968:Escherichia coli Proteins; D007202:Indicators and Reagents; C050221:LacY protein, E coli; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D010750:Phosphoproteins; D027981:Symporters; D011092:Polyethylene Glycols; C021499:lactose permease; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C029022:phosphoenolpyruvate-lactose dependent phosphotransferase system