Follicle-like hemicysts reorganized in monolayer culture of rat thyroid cells in the medium supplemented with isologous serum.
Monolayer culture of the cells isolated from rat thyroid glands formed a number of large and three-dimensional (about 200-microns diameter) hemicysts in media supplemented with isologous and high-concentration serum. The higher was the concentration of isologous serum, the larger was the hemicysts and the higher was the uptake of 131I of hemicysts. Substitution of heterologous serum, such as calf or fetal calf serum, for the isologous serum failed to form hemicysts.
Goto
K
K
Department of Pathology, Tohoku University School of Medicine, Japan.
Hosaka
S
S
Ishii
H
H
Nagura
H
H
D000818:Animals; D001790:Blood Physiological Phenomena; D002417:Cattle / Q000097:blood / Q000196:embryology; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D051381:Rats; D011912:Rats, Inbred ACI / Q000097:blood; D013045:Species Specificity; D013961:Thyroid Gland / Q000166:cytology*; D013972:Thyrotropin / Q000494:pharmacology
D003470:Culture Media; D013972:Thyrotropin