title: A split zinc-finger protein is required for normal yeast growth.
abstract: We have identified a gene that, when present in multiple copies, partially inhibits nuclear protein localization in Saccharomyces cerevisiae. This gene encodes a protein that is a unique member of the Cys2His2 zinc-finger family of DNA-binding proteins. It is designated SFP1 for split finger protein because its two zinc-finger domains are separated from one another by 40 amino acids (aa) as opposed to the usual spacing of 7 or 8 aa for Cys2His2 proteins. Disruption of the SFP1 gene results in slow cell growth, with cells having multiple, nucleated buds.
authors.affiliation: Department of Molecular Biology, Princeton University, NJ 08544.
authors.forename: H
authors.initials: H
authors.lastname: Blumberg
authors.forename: P
authors.initials: P
authors.lastname: Silver
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development*; D029701:Saccharomyces cerevisiae Proteins; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; C117421:SFP1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins