Cloning of cDNA encoding rat TCP-1.
We have isolated and sequenced a cDNA encoding a rat homolog of the mouse t-complex polypeptide 1 (TCP-1). Its deduced gene product is a polypeptide of 556 amino acids, with a predicted Mr of 60,341. The similarity between mouse Tcp-1 and the rat homolog is about 94.0% at the nucleotide level and 97.1% at the amino acid level showing the evolutionary conservation of this protein. The similarity of the amino acid sequence of the rat TCP-1 is not significantly biased to any of those from wild (TCP-1B) or from t-haplotype mice (TCP-1A). From a comparison of deduced amino acid sequences of eukaryotic TCP-1 proteins, we found highly conserved domains. Southern blot analysis revealed that there are at least two similar sequences to Tcp-1 in the rat, one is a structural gene and the other seems to be a processed pseudogene.
Morita
T
T
Department of Microbial Genetics, Osaka University, Japan.
Kubota
H
H
Gachelin
G
G
Nozaki
M
M
Matsushiro
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D011544:Pseudogenes / Q000235:genetics; D051381:Rats / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013735:Testicular Hormones / Q000235:genetics; D044767:Ubiquitin-Protein Ligases; D056427:t-Complex Genome Region
D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D013735:Testicular Hormones; C116196:PPP1R11 protein, human; D044767:Ubiquitin-Protein Ligases