title: Cooperative binding of the globular domains of histones H1 and H5 to DNA.
abstract: In view of the likely role of H1-H1 interactions in the stabilization of chromatin higher order structure, we have asked whether interactions can occur between the globular domains of the histone molecules. We have studied the properties of the isolated globular domains of H1 and the variant H5 (GH1 and GH5) and we have shown (by sedimentation analysis, electron microscopy, chemical cross-linking and nucleoprotein gel electrophoresis) that although GH1 shows no, and GH5 little if any, tendency to self-associate in dilute solution, they bind highly cooperatively to DNA. The resulting complexes appear to contain essentially continuous arrays of globular domains bridging 'tramlines' of DNA, similar to those formed with intact H1, presumably reflecting the ability of the globular domain to bind more than one DNA segment, as it is likely to do in the nucleosome. Additional (thicker) complexes are also formed with GH5, probably resulting from association of the primary complexes, possibly with binding of additional GH5. The highly cooperative nature of the binding, in close apposition, of GH1 and GH5 to DNA is fully compatible with the involvement of interactions between the globular domains of H1 and its variants in chromatin folding.
authors.affiliation: Department of Biochemistry, University of Cambridge, UK.
authors.forename: J O
authors.initials: JO
authors.lastname: Thomas
authors.forename: C
authors.initials: C
authors.lastname: Rees
authors.forename: J T
authors.initials: JT
authors.lastname: Finch
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000502:physiology; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D004247:DNA / Q000378:metabolism* / Q000648:ultrastructure; D004586:Electrophoresis; D004912:Erythrocytes; D006657:Histones / Q000378:metabolism* / Q000648:ultrastructure; D046911:Macromolecular Substances; D008854:Microscopy, Electron
chemical_list: D006657:Histones; D046911:Macromolecular Substances; D004247:DNA