title: Effect of mitomycin C and bromodeoxyuridine on Fanconi anemia lymphocytes.
abstract: The authors studied the effect of mitomycin C (MMC) and bromodeoxyuridine (BrdU) on the induction of chromosome aberrations on lymphocytes of four patients with Fanconi anemia (FA) and of one normal subject. A control culture and six experiments were designed to test the possible synergic effect of MMC and BrdU. Their results revealed no evidence of MMC-BrdU synergism on the induction of chromosome aberrations in FA lymphocytes. However, chromosomes showed more damage when FA cells were harvested 24 h after MMC stress than when cells were harvested shortly after treatment. This can be explained by a DNA repair defect or by a toxic effect of oxygenation of cells during the procedure.
authors.affiliation: Departamento de Genetica Humana, Instituto Nacional de Pediatría, Mexico D.F.
authors.forename: S
authors.initials: S
authors.lastname: Frias
authors.forename: S
authors.initials: S
authors.lastname: Mendoza
authors.forename: B
authors.initials: B
authors.lastname: Molina
authors.forename: A
authors.initials: A
authors.lastname: Carnevale
mesh_terms: D001973:Bromodeoxyuridine / Q000633:toxicity*; D002648:Child; D002869:Chromosome Aberrations / Q000235:genetics*; D005199:Fanconi Anemia / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D016685:Mitomycin / Q000633:toxicity*
chemical_list: D016685:Mitomycin; D001973:Bromodeoxyuridine