Selective regulation of eosinophil degranulation by interleukin 1 beta.
Recent evidence confirms that cytokines such as IL-1, IL-4, IL-5, and GM-CSF may enhance or inhibit eosinophil function. Functions that are susceptible to modulation include eosinophil-mediated antibody-dependent damage of helminthic parasites, oxidative metabolism and degranulation. We have employed IgG and IgE-coated Sepharose beads to investigate selective modulation of IgG and IgE-mediated enzyme release by IL-1 beta. Both IgG and IgE-coated beads induced release of granular enzymes beta-glucuronidase and arylsulfatase. Enzyme release from IgG-stimulated eosinophils was inhibited by preincubation with IL-1 beta (100 pg/ml, P less than or equal to 0.05). In contrast, enzyme release by IgE-stimulated eosinophils was enhanced by IL-1 beta (100 pg/ml, P less than or equal to 0.05). These studies support the hypothesis that IL-1 beta has specific selective actions on eosinophil function. Furthermore, these actions on particle-stimulated enzyme release suggest that IgG and IgE mediated processes in eosinophils are differentially regulated.
Baskar
P
P
Department of Dermatology, New England Medical Center Hospital, Boston, Massachusetts 02111.
Pincus
S H
SH
D001192:Arylsulfatases / Q000378:metabolism; D015550:Cell Degranulation / Q000187:drug effects*; D004804:Eosinophils / Q000502:physiology*; D005434:Flow Cytometry; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D007073:Immunoglobulin E; D007074:Immunoglobulin G; D007375:Interleukin-1 / Q000494:pharmacology*
D007074:Immunoglobulin G; D007375:Interleukin-1; D007073:Immunoglobulin E; D001192:Arylsulfatases; D005966:Glucuronidase