title: Human neutrophil granule cationic protein CAP37 is a specific macrophage chemotaxin that shares homology with inflammatory proteinases.
abstract: Cationic antimicrobial protein CAP37 (Mr = 37 kD) is derived from the azurophilic granules of human PMN. In vitro and in vivo studies demonstrate that CAP37 is a novel monocyte-specific chemoattractant. The N-terminal amino acid sequence of CAP37 shares significant homology with a number of inflammatory molecules with protease activity including elastase and cathepsin G. However, substitutions in the catalytic triad (serine for a histidine at position 41 and glycine for a serine at position 175), may account for its lack of serine protease activity. A full length cDNA for CAP37 was identified in an HL60 cDNA library screened with oligonucleotide probes designed from the N-terminal amino acid sequence. Sequencing of the cDNA reveals a protein of 225 amino acids with significant nucleotide homology to cathepsin G and human neutrophil elastase.
authors.affiliation: Genelabs Inc., Redwood City, CA 94063.
authors.forename: J G
authors.initials: JG
authors.lastname: Morgan
authors.forename: H A
authors.initials: HA
authors.lastname: Pereira
authors.forename: T
authors.initials: T
authors.lastname: Sukiennicki
authors.forename: J K
authors.initials: JK
authors.lastname: Spitznagel
authors.forename: J W
authors.initials: JW
authors.lastname: Larrick
mesh_terms: D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D002352:Carrier Proteins; D002630:Chemotactic Factors; D004247:DNA / Q000235:genetics; D006801:Humans; D007249:Inflammation / Q000201:enzymology*; D008264:Macrophages; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases / Q000737:chemistry*
chemical_list: C062991:AZU1 protein, human; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002352:Carrier Proteins; D002630:Chemotactic Factors; D004247:DNA; D012697:Serine Endopeptidases