[Principles and application possibilities of cryoultramicrotomy].
This paper reviews the technical aspects of cryoultramicrotomy. The key preparation steps in the technique, namely freezing, sectioning, thawing, and staining are analysed. The application of this method to immunocytochemistry and analytical electron microscopy are discussed with respect to the present possibilities and problems.
Paradisi
S
S
Laboratorio di Ultrastrutture, Istituto Superiore di Sanità, Roma.
Arancia
G
G
Donelli
G
G
D016616:Cryoultramicrotomy / Q000379:methods; D004577:Electron Probe Microanalysis / Q000379:methods; D005629:Frozen Sections; D006801:Humans; D008854:Microscopy, Electron / Q000379:methods; D016253:Microscopy, Immunoelectron / Q000379:methods; D013048:Specimen Handling
Principi e possibilità applicative della crioultramicrotomia.