title: Crystallization and preliminary X-ray analysis of botulinum neurotoxin type A.
abstract: Botulinum neurotoxin serotype A was isolated from liquid culture of Clostridium botulinum. The pure Mr approximately 150,000 neurotoxin, composed of Mr approximately 50,000 light and Mr approximately 100,000 heavy chains, has been crystallized in three different crystal morphologies; all three have the same crystal form. The most suitable crystal form for X-ray analysis are bipyrimidal and crystallize in the hexagonal space group P3(1)21 (or P3(2)21) with one dimer per asymmetric unit. The unit cell dimensions are a = b = 170.5 A, c = 161.7 A. The crystals diffract to 3 A resolution.
authors.affiliation: Gibbs Chemical Laboratory, Harvard University, Cambridge, MA 02138.
authors.forename: R C
authors.initials: RC
authors.lastname: Stevens
authors.forename: M L
authors.initials: ML
authors.lastname: Evenson
authors.forename: W
authors.initials: W
authors.lastname: Tepp
authors.forename: B R
authors.initials: BR
authors.lastname: DasGupta
mesh_terms: D001905:Botulinum Toxins / Q000737:chemistry* / Q000302:isolation & purification; D003014:Clostridium botulinum; D003460:Crystallization; D046911:Macromolecular Substances; D008970:Molecular Weight; D009498:Neurotoxins / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D046911:Macromolecular Substances; D009498:Neurotoxins; D001905:Botulinum Toxins