Occurrence of toxic shock syndrome toxin-1 producing Staphylococcus aureus and the anti TSST-1 serostatus of hospital personnel in Nigeria.
Of the 56 S. aureus strains tested, five (8.9%) isolates (four from asymptomatic nasal carriage and one from a non TSS staphylococcal disease) demonstrated TSST-1 production in vitro. Among the toxin positive strains the clinical isolate belonged to phage group I, 29/52 and the nasal carriage isolates were either non typable or ungroupable. Eighty-nine percent of isolates including all TSST-1 positive strains showed binding to crystal violet and were traced to S. aureus var. hominis (CV type C/D). A. aureus var. bovis (CV type A/B) were found only in nasal carriage group (10.7%) and did not bind crystal violet. During the sero-surveillance 101 out of 220 hospital personnel (48.1%) showed TSST-1 antibodies of IgG class at 1:1000 sera dilution. The four individuals with asymptomatic nasal carriage of TSST-1 positive staphylococci showed no antibody to the toxin. Anti TSST-1 levels persisted in 58 (57.4%) and 10 (9.9%) individuals at higher sera dilutions of 10(-4) and 10(-5) respectively.
Olusanya
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O
Department of Pathology, College of Health Sciences, Ogun State University, Sagamu, Nigeria.
Naidu
A S
AS
D000293:Adolescent; D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000097:blood*; D001427:Bacterial Toxins; D001434:Bacteriophage Typing; D002353:Carrier State / Q000097:blood / Q000453:epidemiology* / Q000276:immunology; D004768:Enterotoxins / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D015994:Incidence; D008875:Middle Aged; D009549:Nigeria / Q000453:epidemiology; D010564:Personnel, Hospital; D016036:Seroepidemiologic Studies; D013203:Staphylococcal Infections / Q000097:blood / Q000453:epidemiology* / Q000276:immunology; D013211:Staphylococcus aureus; D018089:Superantigens
D000888:Antibodies, Anti-Idiotypic; D001427:Bacterial Toxins; D004768:Enterotoxins; D007074:Immunoglobulin G; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal