Requirement of protein factors and ATP for the disassembly of the spliceosome after mRNA splicing reaction.
Pre-mRNA splicing reaction occurs in a large ribonucleoprotein complex called the spliceosome. After the splicing reaction, the spliceosome is disassembled to release the splicing products including spliced mRNA. Here we show that protein factors in a HeLa nuclear extract or a DEAE-cellulose fraction as well as ATP are required to release the splicing products form the spliceosome in which the splicing reaction has already completed.
Sawa
H
H
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Shimura
Y
Y
D000255:Adenosine Triphosphate / Q000378:metabolism*; D053487:DEAD-box RNA Helicases; D005656:Fungal Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D020365:RNA Helicases; D012322:RNA Precursors / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional / Q000502:physiology; D012326:RNA Splicing / Q000502:physiology; D000072260:RNA Splicing Factors; D012333:RNA, Messenger / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins
D005656:Fungal Proteins; D009687:Nuclear Proteins; D012322:RNA Precursors; D000072260:RNA Splicing Factors; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D029701:Saccharomyces cerevisiae Proteins; D000255:Adenosine Triphosphate; C067135:PRP22 protein, S cerevisiae; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases