Effect of mitomycin C and bromodeoxyuridine on Fanconi anemia lymphocytes.
The authors studied the effect of mitomycin C (MMC) and bromodeoxyuridine (BrdU) on the induction of chromosome aberrations on lymphocytes of four patients with Fanconi anemia (FA) and of one normal subject. A control culture and six experiments were designed to test the possible synergic effect of MMC and BrdU. Their results revealed no evidence of MMC-BrdU synergism on the induction of chromosome aberrations in FA lymphocytes. However, chromosomes showed more damage when FA cells were harvested 24 h after MMC stress than when cells were harvested shortly after treatment. This can be explained by a DNA repair defect or by a toxic effect of oxygenation of cells during the procedure.
Frias
S
S
Departamento de Genetica Humana, Instituto Nacional de Pediatría, Mexico D.F.
Mendoza
S
S
Molina
B
B
Carnevale
A
A
D001973:Bromodeoxyuridine / Q000633:toxicity*; D002648:Child; D002869:Chromosome Aberrations / Q000235:genetics*; D005199:Fanconi Anemia / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D016685:Mitomycin / Q000633:toxicity*
D016685:Mitomycin; D001973:Bromodeoxyuridine