Binding of peripheral blood and thyroidal T lymphocytes to thyroid cell monolayers: possible role of "homing-like" receptors in the pathogenesis of thyroid autoimmunity.
The specific binding of blood lymphocyte populations to non-antigenic receptors on high endothelial cells in peripheral lymphoid organs is well established. Such "homing" receptors in target tissues may also play a role in the early phase of organ specific autoimmunity. In this study binding of peripheral blood and thyroid-derived T cells to human thyroid (THY) cells was examined. Binding was measured as the percentage 51Cr-labelled T cells bound to THY monolayers. Interferon-gamma (IFN-gamma) enhanced the binding of peripheral blood T cells (PBL-T) from patients with Graves' disease (GD) and normals, but not from patients with Hashimoto's thyroiditis (HT), to allogeneic THY monolayers. The binding of T cells from patients with HT to untreated allogeneic THY monolayers was significantly greater than that for T cells from patients with GD or normals. TSH inhibited the IFN-gamma enhancement of binding of PBL-T from 4 normals and one HT patient to both allogeneic (n = 5) and autologous (n = 4) THY monolayers. Intra-thyroid T cells (ITL-T) bound to autologous THY monolayers significantly more than PBL-T from the same patient, while ITL from patients with HT or Graves' disease bound more than their PBL-T to both allogeneic and autologous THY monolayers. ITL-T, but not PBL-T, bound significantly more to autologous THY than to autologous thyroid-derived fibroblast monolayers.(ABSTRACT TRUNCATED AT 250 WORDS)
Fukazawa
H
H
Thyroid Research Unit, McGill University, Montreal General Hospital, Quebec, Canada.
Hiromatsu
Y
Y
Bernard
N
N
Salvi
M
M
Wall
J R
JR
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D016201:Receptors, Lymphocyte Homing / Q000032:analysis*; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D013961:Thyroid Gland / Q000737:chemistry / Q000276:immunology*; D013967:Thyroiditis, Autoimmune / Q000276:immunology* / Q000503:physiopathology
D000911:Antibodies, Monoclonal; D016201:Receptors, Lymphocyte Homing; D007371:Interferon-gamma