title: Regulation of testicular insulin-like growth factor-I in pubertal growth hormone-deficient male rats.
abstract: GH plays a major role in pubertal growth, effects mainly mediated by stimulation of insulin-like growth factor-I (IGF-I) production by the liver. However, the role of GH in the regulation of pubertal onset, spermatogenesis and fertility is still under debate. GH and FSH have, in addition, been implicated in the regulation of IGF-I production by Sertoli cells in a number of studies, although conflicting results have been reported. The interpretation of studies using GH-deficient mutant mice has been complicated by the presence of additional defects in the hypothalamic-pituitary-gonadal axis of these animals. We have therefore used GH-deficient mutant male rats with no other documented hormonal deficiencies to study the effect of GH administration on somatic and testicular development, circulating and testicular IGF-I concentrations and testicular histology. Body weights in GH-deficient rats substituted with GH were not significantly different from untreated or GH-treated normal rats and were significantly higher than body weights in untreated dwarf rats. Similarly, circulating IGF-I concentrations in GH-treated GH-deficient rats were not significantly different from those in untreated or GH-treated normal rats but were significantly higher than circulating IGF-I concentrations in untreated dwarf rats. No differences in testicular IGF-I concentrations were observed in any of the groups studied. Testicular weights remained low in both untreated and GH-treated GH-deficient animals compared with control animals but spermatogenesis was qualitatively and quantitatively normal in all groups at the end of the observation period.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Max Planck Clinical Research Unit for Reproductive Medicine, Münster, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Spiteri-Grech
authors.forename: J M
authors.initials: JM
authors.lastname: Bartlett
authors.forename: E
authors.initials: E
authors.lastname: Nieschlag
mesh_terms: D000818:Animals; D001835:Body Weight; D005640:Follicle Stimulating Hormone / Q000097:blood; D013006:Growth Hormone / Q000172:deficiency* / Q000494:pharmacology; D007118:Immunoassay / Q000379:methods; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D008297:Male; D009929:Organ Size; D051381:Rats; D011922:Rats, Mutant Strains; D012741:Sexual Maturation / Q000502:physiology*; D013737:Testis / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000097:blood
chemical_list: D013739:Testosterone; D007334:Insulin-Like Growth Factor I; D005640:Follicle Stimulating Hormone; D013006:Growth Hormone