Evidence for a Competitive-Displacement Model for the initiation of protein synthesis involving the intermolecular hybridization of 5 S rRNA, 18 S rRNA and mRNA.
We have previously shown that a 5'-terminal region of mouse 5 S rRNA can base-pair in vitro with two distinct regions of 18 S rRNA. Further analysis reveals that these 5 S rRNA-complementary sequences in 18 S rRNA also exhibit complementarity to the Kozak consensus sequence surrounding the mRNA translational start site. To test the possibility that these 2 regions in 18 S rRNA may be involved in mRNA binding and translational initiation, we have tested, using an in vitro translation system, the effects of DNA oligonucleotides complementary to these 18 S rRNA sequences on protein synthesis. Results show that an oligonucleotide complementary to one 18 S rRNA region does inhibit translation at the step of initiation. We propose a Competitive-Displacement Model for the initiation of translation involving the intermolecular base-pairing of 5 S rRNA, 18 S rRNA and mRNA.
Sarge
K D
KD
Department of Biochemistry, NCSU, Raleigh 27695-7622.
Maxwell
E S
ES
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000494:pharmacology; D005914:Globins / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics*; D012337:RNA, Ribosomal, 18S / Q000737:chemistry / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000737:chemistry / Q000235:genetics*; D011817:Rabbits; D051381:Rats; D012969:Sodium Fluoride / Q000494:pharmacology
D012333:RNA, Messenger; D012337:RNA, Ribosomal, 18S; D012341:RNA, Ribosomal, 5S; D012969:Sodium Fluoride; D005914:Globins; D004247:DNA