title: The interaction of heparinized biomaterials with human serum, albumin, fibrinogen, antithrombin III, and platelets.
abstract: The influence of the method of heparin (HEP) immobilization on human serum albumin (HSA), fibrinogen (FG), and antithrombin III (AT-III) adsorption, platelet adhesion, and activation on the surface of polyvinylchloride, polyurethane Vitur, and a copolymer of styrene and divinylbenzene was measured. The negative correlation between the degree of irreversibility of plasma protein adsorption and the amount of adsorbed AT-III for HEP, immobilized onto the polymer surface passivated with HSA, FG, and plasma was found. The same negative correlation was observed between the amount of AT-III adsorbed on these systems and the number of adhered platelets. Schemes of the interaction of surface bound-HEP with AT-III, including the influence of an irreversibly adsorbed protein layer and adhered platelets, have been proposed. The essential role of AT-III in heparinized biomaterials/platelet interaction has been shown. A new method of combined immobilization of HEP and platelet adhesion inhibitor has been elaborated on.
authors.affiliation: Biomaterials Center, Institute of Transplantology and Artificial Organs, Moscow, U.S.S.R.
authors.forename: E A
authors.initials: EA
authors.lastname: Nemets
authors.forename: V I
authors.initials: VI
authors.lastname: Sevastianov
mesh_terms: D000990:Antithrombin III; D001672:Biocompatible Materials / Q000494:pharmacology*; D001792:Blood Platelets; D001798:Blood Proteins; D002404:Catheterization / Q000295:instrumentation; D005340:Fibrinogen; D006493:Heparin; D006801:Humans; D066298:In Vitro Techniques; D015539:Platelet Activation; D010973:Platelet Adhesiveness; D011140:Polyurethanes; D011143:Polyvinyl Chloride; D012709:Serum Albumin; D020058:Styrene; D013343:Styrenes; D014753:Vinyl Compounds
chemical_list: D001672:Biocompatible Materials; D001798:Blood Proteins; D011140:Polyurethanes; D012709:Serum Albumin; D013343:Styrenes; D014753:Vinyl Compounds; D020058:Styrene; D000990:Antithrombin III; D005340:Fibrinogen; D011143:Polyvinyl Chloride; D006493:Heparin; C004985:divinyl benzene