Induction of DNA polymerase beta during proliferation of mitogen-stimulated human lymphocytes.
On induction of proliferation of human peripheral blood mononuclear cells by phytohemagglutinin treatment, DNA polymerase beta activity increases markedly before and during DNA replication. The increase of enzymatic activity seems to be well correlated with the increase of DNA polymerase beta mRNA, which is induced by enhanced expression of the DNA polymerase beta gene. These data suggest that DNA polymerase beta is involved in DNA repair, which is linked to replicative DNA synthesis, or directly in replicative DNA synthesis in normal proliferating cells.
Suzuki
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Istituto di Chimica Biologica, Università di Verona, Italy.
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D015152:Blotting, Northern; D002455:Cell Division; D004256:DNA Polymerase I / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology* / Q000201:enzymology; D010835:Phytohemagglutinins; D012333:RNA, Messenger / Q000378:metabolism
D010835:Phytohemagglutinins; D012333:RNA, Messenger; D004256:DNA Polymerase I