Establishment of an estrogen receptor-positive cell line (HMA-1) derived from human breast carcinoma.
We have established a novel human breast carcinoma cell line, HMA-1, derived from ascites of a female breast cancer patient. HMA-1 was shown to be an epithelial cell line with intracytoplasmic duct-like vacuoles, microvilli, desmosomes and tonofibrils in accordance with human breast cancer. The cell line demonstrated a good cell growth ability in monolayer fashion with a doubling time of 46 hr. Based on a whole cell binding assay the cell line contained estrogen receptor (1.45 x 10(-4) sites/cell). Tamoxifen, an anti-estrogen agent induced a dose-dependent decrease in the cell growth rate, but estradiol stimulated the cell growth. HMA-1 could be transplanted subcutaneously into BALB/c nude mice, and was able to cause tumors approximately two months after heteroinoculation. These results indicate that HMA-1 cell line may serve as a new human breast carcinoma cell line which could be utilized in the breast cancer research.
Matano
S
S
Second Department of Surgery, Tohoku University School of Medicine, Sendai, Japan.
Ohuchi
N
N
Hirakawa
H
H
Nishihira
T
T
Abe
M
M
Mori
S
S
Akimoto
M
M
D000230:Adenocarcinoma / Q000737:chemistry* / Q000648:ultrastructure; D000818:Animals; D001943:Breast Neoplasms / Q000737:chemistry* / Q000648:ultrastructure; D002455:Cell Division; D002460:Cell Line; D005260:Female; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009368:Neoplasm Transplantation; D011960:Receptors, Estrogen / Q000032:analysis*; D014407:Tumor Cells, Cultured
D011960:Receptors, Estrogen