Using PCR to extend the limit of oligonucleotide synthesis.
A method has been developed to allow one to extend the practical limit of oligonucleotide synthesis by coupling the synthesis reaction to a subsequent PCR. Given that DNA synthesizers are capable of producing reasonable yields of oligonucleotides that are 125-150 bases in length, this method could be used to recover the minute amount of full-length product present in mixtures extended well beyond the established limits. This technology could be applied to gene synthesis and mutagenesis.
Michaels
M L
ML
Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024.
Hsiao
H M
HM
Miller
J H
JH
D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D016133:Polymerase Chain Reaction; D019914:Taq Polymerase; D013698:Templates, Genetic
D009841:Oligonucleotides; D004247:DNA; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase