Interleukin 1 protects hair follicles from cytarabine (ARA-C)-induced toxicity in vivo and in vitro.
ImuVert, a biologic response modifier, and interleukin 1 (IL 1) have been shown to protect the young rat from alopecia induced by cytarabine (ARA-C). In the present study the inhibition by ARA-C of DNA synthesis in hair follicles (HFs) and the protective effect of ImuVert and IL 1 were investigated in vivo and in vitro. Both ImuVert and IL 1 were equally effective in protecting rats from ARA-C-induced alopecia. DNA synthesis in HFs isolated from ARA-C-treated animals was 10-20% of untreated controls. Follicles isolated from animals given either ImuVert or IL 1 before ARA-C exhibited normal DNA synthesis. In vitro, the incubation of normal rat HF with ARA-C resulted in 80% inhibition of [3H]-thymidine uptake. Preincubation of the follicles for 1 hr with IL 1 before the addition of ARA-C completely blocked the inhibition. Preincubation with imuVert, however, was less effective in blocking the inhibition from ARA-C.
Jimenez
J J
JJ
Department of Medicine, University of Miami School of Medicine, Florida 33101.
Sawaya
M E
ME
Yunis
A A
AA
D000505:Alopecia / Q000139:chemically induced / Q000188:drug therapy*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001688:Biological Products; D003561:Cytarabine / Q000633:toxicity*; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004336:Drug Antagonism; D006197:Hair / Q000187:drug effects*; D007155:Immunologic Factors / Q000494:pharmacology; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D000970:Antineoplastic Agents; D001688:Biological Products; D007155:Immunologic Factors; C062792:ImuVert; D007375:Interleukin-1; D003561:Cytarabine