Extracellular flush solution that contains blood, mannitol, albumin, and prostacyclin protects rat lungs from six hours of ischemia.
We have used an isolated rat lung model to compare the quality of preservation of different flush techniques with each other and with topical cooling alone. Lung injury was assessed by recording lung weights after reperfusion after 4 and 6 hours of ischemia. The flush solutions studied were intracellular (Collins-Sacks), traditional extracellular, extracellular with low potassium plus dextran, and extracellular containing blood, mannitol, albumin, and prostacyclin (Wallwork's solution). Flushing with Wallwork's solution before both 4 and 6 hours of ischemia gave superior protection from lung edema after reperfusion over all the other methods.
Mulvin
D W
DW
Department of Surgery, University of Colorado Health Sciences Centre, Denver 80262.
Jones
D K
DK
Howard
R B
RB
Grosso
M A
MA
Repine
J E
JE
Johnston
M R
MR
D000418:Albumins; D000818:Animals; D001769:Blood; D003080:Cold Temperature; D011464:Epoprostenol; D008168:Lung; D008353:Mannitol; D009926:Organ Preservation / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000473:pathology; D012996:Solutions
D000418:Albumins; D012996:Solutions; D008353:Mannitol; D011464:Epoprostenol