Improvement of fertilizing performance by normal and abnormal mouse semen after zona opening of mature oocytes.
The usefulness of opening the zona pellucida by partial zona dissection (PZD) to enhance fertilization of mature mouse oocytes was studied after insemination with three types of semen: normal and diluted semen and semen from long-term-vasectomized males. Zona opening did not by itself induce parthenogenetic cleavage of mature oocytes and did not significantly increase mono- and polyspermic fertilization of oocytes inseminated with normal semen. While a fertilization rate of 62% was obtained among intact oocytes, of which 4.5% were polyspermic, a 66.8% fertilization rate was observed among PZD oocytes, 6.3% of which were polyspermic. However, after using diluted semen, only 54 of 193 intact oocytes were fertilized (28%), and PZD improved the fertilization rate to 65.4%. Cleavage rate of nonmanipulated oocytes inseminated with abnormal semen from vasectomized males was dramatically decreased in comparison with those inseminated with normal semen (7.6% vs. 65%). PZD induced a moderate but significant improvement of fertilization performance when using this abnormal semen (19.6%).
Barlow
P
P
IVF Clinic, Saint Pierre Hospital, Brussels, Belgium.
Van Der Zwalmen
P
P
Schoysman
R
R
Leroy
F
F
D000818:Animals; D004624:Embryo Transfer; D005307:Fertilization in Vitro; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008846:Micromanipulation; D009865:Oocytes / Q000502:physiology; D012661:Semen / Q000502:physiology*; D014659:Vasectomy; D015044:Zona Pellucida / Q000502:physiology*