Isolation and characterization of a monoclonal anti CK-2 alpha subunit antibody of the IgG1 subclass.
A monoclonal antibody was produced against the recombinant human alpha subunit of CK-2. The antibody was of the IgG1 subclass and it was isolated from serum-free cell culture media and purified by affinity chromatography on Protein G Sepharose. The antibody can be used to detect specifically the CK-2 alpha subunit in immunoblots from tissue extracts. An ELISA detection test was also established which also allows the identification of the CK-2 alpha subunit.
Schmidt-Spaniol
I
I
Institut für Humangenetik, Universität des Saarlandes, Homburg, Germany.
Boldyreff
B
B
Issinger
O G
OG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D047388:Casein Kinases; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D011494:Protein Kinases / Q000276:immunology* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D011494:Protein Kinases; D047388:Casein Kinases