[The role of monoamine oxidase in the regulation of mitochondrial energy functions].
Incubation of aldehyde dehydrogenase-free mitochondrial preparations with biogenic amines serotonin, tyramine, 2-phenylethylamine and 5-methoxytryptamine resulted in inhibition of enzymes activity of both outer (rotenone-insensitive NADH-cytochrome c reductase) and inner (succinate dehydrogenase, succinate cytochrome c reductase) mitochondrial membranes. Solubilization of mitochondria after the incubation did not influence the amine-induced alteration of succinate dehydrogenase activity. Pretreatment of the organelles with a mixture containing chlorgyline and deprenyl completely inhibited monoamine oxidase (MAO) activity and prevented the effects of all the amines studied on mitochondrial enzymes. MAO-dependent effects of 5-methoxytryptamine were fully reproduced by 5-methoxyindolyl-3-acetaldehyde (one of probable products of 5-methoxytryptamine deamination). The effect of the aldehyde was not prevented by chlorgyline and deprenyl. After selective inhibition of MAO-A by chlorgyline the order of MAO-B-dependent effects of biogenic amines on mitochondrial enzymes studied was as follows: tyramine greater than or equal to 2-phenylethylamine much greater than serotonin. In deprenyl pretreated mitochondria the potency of MAO-A-dependent effects of these amines was: serotonin greater than tyramine much greater than much greater than 2-phenylethylamine. The data obtained suggest that the product(s) of oxidative deamination of biogenic amines (probably the aldehydes) catalyzed by both types of MAO (MAO-A and MAO-B) are able to regulate the energy functions of mitochondria.
Medvedev
A E
AE
Gorkin
V Z
VZ
D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism; D003010:Clorgyline / Q000494:pharmacology; D003902:Detergents; D004734:Energy Metabolism; D007425:Intracellular Membranes / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology* / Q000378:metabolism; D008995:Monoamine Oxidase / Q000502:physiology*; D008996:Monoamine Oxidase Inhibitors / Q000378:metabolism; D017830:Octoxynol; D011092:Polyethylene Glycols; D051381:Rats
D001679:Biogenic Amines; D003902:Detergents; D008996:Monoamine Oxidase Inhibitors; D011092:Polyethylene Glycols; D017830:Octoxynol; D008995:Monoamine Oxidase; D003010:Clorgyline
Rol' monoaminoksidaz v reguliatsii énergeticheskikh funktsiĭ mitokhondriĭ.