Comparison of automated liquid chromatographic and bioassay methods for determining spiramycin concentration in bovine plasma.
The performance of a liquid chromatographic (LC) method for spiramycin measurement in bovine plasma has been compared with that of a microbiological method. Plasma samples were obtained from cattle administered spiramycin intravenously. Comparison tests used were intraclass correlation (r1), correlation (r), and Student's paired t-test. For concentrations lower than 2.5 IU/mL, microbiological values were higher than LC values. This difference in results modified pharmacokinetic interpretation and might be explained by the presence of microbiologically active metabolites.
Sanders
P
P
Ministère de l'Agriculture et de la Forêt, Centre National d'Etudes Vétérinaires et Alimentaires, La Haute Marche-Javené, Fougeres, France.
Moulin
G
G
Gaudiche
C
C
Delepine
B
B
Mourot
D
D
D000818:Animals; D001681:Biological Assay / Q000706:statistics & numerical data; D002417:Cattle / Q000097:blood*; D002853:Chromatography, Liquid / Q000706:statistics & numerical data; D012044:Regression Analysis; D012505:Sarcina / Q000187:drug effects / Q000254:growth & development; D015572:Spiramycin / Q000097:blood* / Q000493:pharmacokinetics / Q000494:pharmacology
D015572:Spiramycin