[Cryopreservation of mouse spermatozoa].
The spermatozoa of cauda epididymis of mature mice were suspended in 3% skim milk in distilled water supplemented with 12, 15, 18 or 21% (W/V) raffinose. The suspension of spermatozoa were frozen in liquid nitrogen gas for 10 min, then stored in liquid nitrogen (-196 degrees C). The frozen suspensions of spermatozoa were thawed by rapid warming in water bath at room temperature. For removing the cryopreservative solution, a pair of syringes connected with a three stop cock and a filter unit (pore size 0.45 mu) were used. Highest sperm motility was obtained after 1 hr of thawing from the cryopreservative solution containing 18% raffinose and 3% skim milk. These cryopreserved spermatozoa were used for fertilization in vitro. The proportion of pronuclear oocytes was 35.9% (74/206) 6 hr after insemination, and the proportion of 2-cell embryos was 33.6% (42/125) 28 hr after insemination. All 2-cell embryos were transferred to the oviducts of pseudopregnant recipients and 45.2% (19/42) developed to normal young.
Takeshima
T
T
Imamichi Institute for Animal Reproduction, Ibaraki, Japan.
Nakagata
N
N
Ogawa
S
S
D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D008297:Male; D051379:Mice; D011247:Pregnancy; D011887:Raffinose; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology
D011887:Raffinose