Ultrastructural localization of the nuclear protein mitotin during the cell cycle.
The work presents the results of the immunoelectron microscopical localization of mitotin in different phases of the cell cycle. The distribution of the protein was studied using its specific monoclonal antibody and immunogold labeling in synchronized WISH cells. In S phase the antigen was found in the nucleoplasm usually over the interchromatin granules. In G2 phase the amount of mitotin increases and it can be found also in the nucleolus. In mitosis the immunogold granules are always out of the condensed chromosomes.
Roussev
R
R
Institute of General and Comparative Pathology, Bulgarian Academy of Sciences, Sofia.
Philipova
R N
RN
Todorov
I T
IT
D000911:Antibodies, Monoclonal; D002453:Cell Cycle; D002466:Cell Nucleolus / Q000737:chemistry / Q000648:ultrastructure; D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure*; D002478:Cells, Cultured; D002843:Chromatin / Q000737:chemistry / Q000648:ultrastructure; D016195:G2 Phase; D006801:Humans; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron / Q000379:methods; D008938:Mitosis; D009687:Nuclear Proteins / Q000032:analysis / Q000648:ultrastructure*; D016196:S Phase
D000911:Antibodies, Monoclonal; D002843:Chromatin; D009687:Nuclear Proteins