title: The influence of extracellular matrix components on the proliferation and migration of inner cell mass-derived parietal endodermal cells.
abstract: Reichert's membrane is a basement membrane deposited on the inner surfaces of rat and mouse trophectodermal (TE) cells beginning at the blastocyst stage of embryonic development that may play a role in the migration of the parietal endodermal (PE) cells to form an inner lining to the TE. The abilities of various glycoproteins present in Reichert's membrane to support PE cell migration and replication in vitro were examined by isolating inner cell masses (ICMs) from Day 5 rat blastocysts (Day 1 = day of vaginal plug) and culturing them (24-72 h) either on surfaces that had been precoated with collagen IV, fibronectin, or laminin or on thin (1-2-mm) gels of Matrigel (a tumor cell-derived basement membrane preparation) or type I collagen. Time-dependent changes in the area occupied by each ICM on the culture surface and the number of migrating cells per ICM were quantified by morphometric analysis. Type IV collagen, the basement membrane-specific collagen, supported ICM attachment and the outward migration (overall increase of approx. 60-fold in mean ICM area occupied on the culture surface) and proliferation (cell doublings following every 24 h of culture) of laminin-containing PE-like cells. These effects were not altered by the inclusion of exogenous fibronectin or laminin in the culture medium. Collagen IV coating concentrations as low as 0.16 micrograms/ml supported PE cell attachment and migration, and maximal responses were seen with a coating concentration of 0.63 micrograms/ml.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Obstetrics & Gynecology, Loeb Institute of Medical Research, Ottawa Civic Hospital, Ottawa, Ontario, Canada.
authors.forename: J A
authors.initials: JA
authors.lastname: Carnegie
authors.forename: O
authors.initials: O
authors.lastname: Cabaca
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000737:chemistry / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D003094:Collagen / Q000494:pharmacology; D004338:Drug Combinations; D047108:Embryonic Development; D004707:Endoderm / Q000502:physiology*; D005260:Female; D005353:Fibronectins / Q000494:pharmacology; D007797:Laminin / Q000494:pharmacology; D011247:Pregnancy; D011509:Proteoglycans / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004338:Drug Combinations; D005353:Fibronectins; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen