[Local immunization against P. aeruginosa with an outer-membrane-protein vaccine].
Averting initial colonization of the respiratory tract with P. aeruginosa would be of great benefit for patients with cystic fibrosis (CF). Our approach to this problem is mucosal immunization with a vaccine prepared from the OMP fraction of a PAO-1 strain of P. aeruginosa. Sprague-Dawley rats were given 5 intragastric doses of the vaccine on 5 consecutive days and an intranasal booster dose 21 days later. Immunized animals developed high titers of OMP-specific IgG antibodies in serum and a specific IgA response in bronchioalveolar and small intestinal lavage samples, all determined by ELISA. When challenged 7 days after the booster (day 28) by intratracheal injection of live bacteria of a heterologous strain of P. aeruginosa the immunized rats showed enhanced bronchopulmonary bacterial clearance compared to nonimmunized controls, as indicated by bacterial counts from homogenized lung tissue taken 4 hrs after challenge. Thus, mucosal immunization with OMP vaccines might hinder initial colonisation of the lungs with P. aeruginosa.
Grundmann
T
T
Abt. für Pädiatrische Pneumologie, Medizinische Hochschule Hannover.
Freihorst
J
J
Kubesch
P
P
Tümmler
B
B
von der Hardt
H
H
D000281:Administration, Intranasal; D000284:Administration, Oral; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D008297:Male; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D012137:Respiratory System / Q000382:microbiology
D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines
Lokale Immunisierung gegen P. aeruginosa mit einer Outer-Membrane-Protein (OMP)-Vakzine.