title: DNA ploidy, S phase fraction and G2 fraction as prognostic determinants in prostatic adenocarcinoma.
abstract: The results of DNA flow cytometry (FCM), histological features and clinical stage of prostatic adenocarcinoma were correlated to outcome in 91 patients during a mean follow-up period of 15.6 years. Aneuploidy was detected in 33 (36%) tumours, and 59 (64%) tumours were diploid. Eighteen (20%) tumours showed a tetraploid DNA index. The frequency of tetraploidy increased towards high-stage and high-grade tumours. Aneuploidy, high S phase fraction (SPF) and G2 fraction were significantly related to clinical stage, histological grade and perineural infiltration. Progressing tumours (T category) had higher SPF values (p = 0.0248), and progression in N (p = 0.0122) and M categories (p = 0.0021) was related to high G2 fraction as well. In T1-2 tumours, DNA ploidy (p = 0.0280) and SPF (p = 0.0230) predicted progression, whereas histological grade had no significant predictive value. The clinical stage (T) predicted crude survival (p = 0.0005). Results show that FCM gives prognostic information in prostatic adenocarcinoma beyond that of histological grading.
authors.affiliation: Department of Surgery, University of Helsinki, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Eskelinen
authors.forename: P
authors.initials: P
authors.lastname: Lipponen
authors.forename: R
authors.initials: R
authors.lastname: Majapuro
authors.forename: K
authors.initials: K
authors.lastname: Syrjänen
authors.forename: S
authors.initials: S
authors.lastname: Nordling
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology*; D000368:Aged; D000369:Aged, 80 and over; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D016195:G2 Phase; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011003:Ploidies; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology*; D016196:S Phase
chemical_list: D004273:DNA, Neoplasm