A GM1b-derived disialoganglioside GD1c is the predominant ganglioside of rat thymocytes.
The thin-layer chromatographic (TLC) pattern of gangliosides of rat thymocytes showed a profile characterized by the occurrence of a predominant ganglioside which did not correspond to any reference gangliosides of rat brain. The ganglioside was isolated from rat thymus, and characterized by compositional analysis, methylation analysis, sialidase treatment, negative-ion fast atom bombardment (FAB) mass spectrometry, and proton NMR spectroscopy. The structure was elucidated to be NeuGc alpha 2-8NeuGc alpha 2-3Gal beta 1-3GalNac beta 1-4Gal beta 1-4Glc beta 1-1Cer. This is the major ganglioside of rat thymus lymphoid cells and is one of the GM1b-derived gangliosides, GD1c, having two N-glycolylneuraminic acids. This is the first report on the occurrence of GD1c in normal animal cells.
Nohara
K
K
National Institute for Environmental Studies, Ibaraki.
Suzuki
M
M
Inagaki
F
F
Kaya
K
K
D000818:Animals; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005677:G(M1) Ganglioside / Q000378:metabolism*; D005732:Gangliosides / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D011919:Rats, Inbred Strains; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism*
D005732:Gangliosides; C072151:ganglioside, GD1c; D005677:G(M1) Ganglioside