title: Molecular analysis of Xenopus laevis SPARC (Secreted Protein, Acidic, Rich in Cysteine). A highly conserved acidic calcium-binding extracellular-matrix protein.
abstract: SPARC (Secreted Protein, Acidic, Rich in Cysteine) is expressed as a 1.6 kb mRNA in Xenopus laevis. On the basis of cDNA sequence analysis, Xenopus SPARC has a core Mr of 32643, with one potential N-glycosylation site. Western analysis of SPARC isolated from Xenopus long bone indicates that the mature protein has an Mr of 43,000. At the amino acid level, Xenopus SPARC has 78-79% sequence similarity to mouse, bovine and human SPARC. The least-conserved region is found within the N-terminal glutamic acid-rich domain, with the C-terminal Ca(2+)-binding domain being the most conserved. Adult Xenopus tissues show the same pattern of tissue-specific distribution of SPARC mRNAs as adult mouse.
authors.affiliation: Department of Zoology, University of Toronto, Ontario, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Damjanovski
authors.forename: F
authors.initials: F
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Ringuette
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015153:Blotting, Western; D004247:DNA; D016326:Extracellular Matrix Proteins / Q000737:chemistry* / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D015676:Osteonectin / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation; D014982:Xenopus laevis
chemical_list: D016326:Extracellular Matrix Proteins; D015676:Osteonectin; D004247:DNA