title: [Isolation of desmosomes: a marker for epithelial tumors].
abstract: A simple method is reported for the isolation of desmosomes. The fresh noncornified layers of cow nose epidermis were immersed in 0.1 mol/L citric acid-sodium citrate buffer (pH 2.3, containing 0.5 mmol/L PMSF), and then treated with homogenization and discontinuous sucrose density gradient centrifugation. Desmosomes were located at the 50-56% sucrose interface. Electron microscopy revealed that the characteristic desmosome structure was well preserved, and that a few intermediate filament bundles attached to desmosome plaque were removed by the solubilizing action of the buffer. Approximate 100 mg desmosomes (dry weight) were got from 20g wet noncornified layers of epidermic tissue.
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: M
authors.initials: M
authors.lastname: Mao
authors.forename: K
authors.initials: K
authors.lastname: Liu
authors.forename: G
authors.initials: G
authors.lastname: Yang
authors.forename: Z
authors.initials: Z
authors.lastname: Sun
authors.forename: X
authors.initials: X
authors.lastname: Lin
mesh_terms: D014408:Biomarkers, Tumor / Q000302:isolation & purification*; D002469:Cell Separation; D003598:Cytoskeletal Proteins / Q000302:isolation & purification*; D051180:Desmoplakins; D003896:Desmosomes; D004817:Epidermis / Q000737:chemistry; D009666:Nose / Q000737:chemistry
chemical_list: D014408:Biomarkers, Tumor; D003598:Cytoskeletal Proteins; D051180:Desmoplakins