title: Effect of chronic microwave radiation on T cell-mediated immunity in the rabbit.
abstract: Experiments were conducted to elucidate the effects of chronic low power-level microwave radiation on the immunological systems of rabbits. Fourteen male Belgian white rabbits were exposed to microwave radiation at 5 mW/cm2, 2.1 GHz, 3 h daily, 6 days/week for 3 months in two batches of 7 each in specially designed miniature anechoic chambers. Seven rabbits were subjected to sham exposure for identical duration. The microwave energy was provided through S band standard gain horns connected to a 4K3SJ2 Klystron power amplifier. The first batch of animals were assessed for T lymphocyte-mediated cellular immune response mechanisms and the second batch of animals for B lymphocyte-mediated humoral immune response mechanisms. The peripheral blood samples collected monthly during microwave/sham exposure and during follow-up (5/14 days after termination of exposures, in the second batch animals only) were analysed for T lymphocyte numbers and their mitogen responsiveness to ConA and PHA. Significant suppression of T lymphocyte numbers was noted in the microwave group at 2 months (P less than 0.01, delta % 21.5%) and during follow-up (P less than 0.01, delta % 30.2%). The first batch animals were initially sensitised with BCG and challenged with tuberculin (0.03 ml) at the termination of microwave irradiation/sham exposure and the increase in foot pad thickness (delta mm), which is a measure of T cell-mediated immunity (delayed type hypersensitivity response, DTH) was noted in both the groups. The microwave group revealed a better response than the control group (delta % +12.4 vs. +7.54). The animals were sacrificed and the tissue T lymphocyte counts (spleen and lymph node) were analysed.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Defense Institute of Physiology and Allied Sciences, Delhi Cantt., India.
authors.forename: K S
authors.initials: KS
authors.lastname: Nageswari
authors.forename: K R
authors.initials: KR
authors.lastname: Sarma
authors.forename: V S
authors.initials: VS
authors.lastname: Rajvanshi
authors.forename: R
authors.initials: R
authors.lastname: Sharan
authors.forename: M
authors.initials: M
authors.lastname: Sharma
authors.forename: V
authors.initials: V
authors.lastname: Barathwal
authors.forename: V
authors.initials: V
authors.lastname: Singh
mesh_terms: D000818:Animals; D007111:Immunity, Cellular / Q000528:radiation effects; D007958:Leukocyte Count; D008297:Male; D008872:Microwaves / Q000009:adverse effects*; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology / Q000528:radiation effects*