The existence of galactosylceramide I3-sulfate in serums of various mammals and its anticoagulant activity.
Human, porcine, goat, sheep, bovine, horse, canine, rat, mouse, guinea pig, and chicken serums were investigated for the existence of sulfatide. Among the ten mammal serums, seven were found to be sulfatide positive, and the amounts of sulfatide were determined to be: 16.29 nmol/ml serum (porcine), 9.39 (bovine), 12.71 (goat), 7.75 (horse), 1.21 (sheep), 0.64 (human), and 0.16 (dog). The existence of sulfatide in the serums of human, goat, sheep, cow, horse, and dog is here reported for the first time. It is suggested that sulfatide is widely distributed in the serums of various mammals except for rodents and that it takes part in the anticoagulant systems. The fatty acids of those sulfatides comprised mainly non-hydroxy fatty acids and a significant amount (18-53% of the total fatty acid) of hydroxy fatty acids with chain lengths of C16, C22, C23, and C24. The long chain bases comprised sphingenine, sphinganine, and 4-D-hydroxysphinganine. Experiments to elucidate the mechanism of the anticoagulant activity of sulfatide revealed that it was specific to sulfatide and that the galactose-bound sulfate group is essential for this activity. The activity of clusters of sulfatide molecules was much more pronounced than that of single molecules.
Zhu
X H
XH
Department of Lipid Biochemistry, Institute of Cardiovascular Disease, Shinshu University School of Medicine, Matsumoto, Nagano.
Hara
A
A
Taketomi
T
T
D000818:Animals; D000925:Anticoagulants; D002855:Chromatography, Thin Layer; D005337:Fibrin / Q000032:analysis; D005699:Galactosylceramides / Q000097:blood* / Q000494:pharmacology; D008322:Mammals; D010084:Oxidation-Reduction; D013433:Sulfoglycosphingolipids / Q000097:blood*
D000925:Anticoagulants; D005699:Galactosylceramides; D013433:Sulfoglycosphingolipids; D005337:Fibrin