title: Ultrastructural localization of dystrophin in chicken smooth muscle.
abstract: We investigated the presence of dystrophin in gizzard smooth muscle by immunofluorescence assay, immunoblot detection and an immunogold electron microscopy technique. Western blot analyses, using antibodies raised against sequences 1173-1728 and 3357-3660 of the dystrophin molecule, revealed the presence of a major intact 400 kDa protein band and an immunofluorescence localization restricted to the periphery of the smooth muscle cells. We were able to precisely determine the dystrophin distribution along the plasmalemma whereas caldesmon molecules were present in the cytoplasm. The most commonly observed distance between two neighbouring dystrophin molecules suggested a self-associating arrangement. We discuss these findings in relation to the function of dystrophin in the smooth muscle cell structure.
authors.affiliation: Centre de Biochimie Macromoléculaire, Université de Montpellier I, INSERM U.249, CNRS-UPR 8402, France.
authors.forename: M C
authors.initials: MC
authors.lastname: Harricane
authors.forename: N
authors.initials: N
authors.lastname: Augier
authors.forename: J
authors.initials: J
authors.lastname: Leger
authors.forename: M
authors.initials: M
authors.lastname: Anoal
authors.forename: C
authors.initials: C
authors.lastname: Cavadore
authors.forename: D
authors.initials: D
authors.lastname: Mornet
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002148:Calmodulin-Binding Proteins / Q000032:analysis; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D003593:Cytoplasm / Q000737:chemistry; D016189:Dystrophin / Q000032:analysis* / Q000502:physiology; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D009130:Muscle, Smooth / Q000737:chemistry* / Q000502:physiology / Q000648:ultrastructure; D013329:Structure-Activity Relationship
chemical_list: D000911:Antibodies, Monoclonal; D002148:Calmodulin-Binding Proteins; D016189:Dystrophin