Highly bioluminescent Bacillus subtilis obtained through high-level expression of a luxAB fusion gene.
Bioluminescence levels comparable to those achievable in Escherichia coli have yet to be obtained from luxAB expression in gram-positive bacteria. In this communication we describe the gene engineering required to generate a highly bioluminescent derivative of Bacillus subtilis. The combination of a powerful promoter, Pxyn, a fusion derivative of luxAB from Vibrio harveyi and translational coupling have overcome the previously reported limitations in luxAB expression. The implications for highly bioluminescent gram-positive organisms are discussed.
Jacobs
M
M
Department of Microbiology, Technical University of Denmark, Lyngby.
Hill
P J
PJ
Stewart
G S
GS
D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004269:DNA, Bacterial; D005784:Gene Amplification; D005798:Genes, Bacterial; D007700:Kinetics; D008156:Luciferases / Q000235:genetics*; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis
D003062:Codon; D004269:DNA, Bacterial; D008156:Luciferases