title: Mycobacterium paratuberculosis antigen D: characterization and evidence that it is a bacterioferritin.
abstract: By using a combination of agarose and polyacrylamide gel electrophoresis, Mycobacterium paratuberculosis antigen D was resolved from a crude sonicated preparation of the organism and characterized as a component with a molecular mass of approximately 400,000 Da. While this component was composed mainly of protein, with unusually high proportions of glutamic acid and leucine, it was resistant to digestion with a number of proteolytic enzymes. Structural detail revealed by electron microscopy, amino acid sequence data, and the demonstration of a Soret band in its absorption spectrum indicated that antigen D was similar to an Escherichia coli bacterioferritin.
authors.affiliation: Agriculture Canada, Animal Diseases Research Institute, Nepean, Ontario, Canada.
authors.forename: B W
authors.initials: BW
authors.lastname: Brooks
authors.forename: N M
authors.initials: NM
authors.lastname: Young
authors.forename: D C
authors.initials: DC
authors.lastname: Watson
authors.forename: R H
authors.initials: RH
authors.lastname: Robertson
authors.forename: E A
authors.initials: EA
authors.lastname: Sugden
authors.forename: K H
authors.initials: KH
authors.lastname: Nielsen
authors.forename: S A
authors.initials: SA
authors.lastname: Becker
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins; D003573:Cytochrome b Group / Q000032:analysis / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005293:Ferritins / Q000032:analysis / Q000235:genetics; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology*; D012689:Sequence Homology, Nucleic Acid; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003573:Cytochrome b Group; C072165:antigen D, Mycobacterium paratuberculosis; D005293:Ferritins; C018032:bacterioferritin