Acetylcholine release in experimental autonomic neuropathy.
Autonomic neuropathy is a common complication of diabetes. In this study we evaluated autonomic neuropathy by determining somatostatin (S-14)-evoked acetylcholine (Ach) release from postsynaptic parasympathetic fibers in the atria of controls (C) and streptozotocin diabetic rats (STZ-D), with and without tetrodotoxin (TTX). The release induced by S-14 did not differ in C and STZ-D. TTX blocked S-14 induced Ach in C but failed in STZ-D. TTX resistance in STZ-D may be explained by variations of membrane potential in nerve fibers.
Uccioli
L
L
Institute of Metabolism Disorders, Tor Vergata University, Rome, Italy.
Magnani
P
P
Tilli
P
P
Cotroneo
P
P
Manto
A
A
Mancini
L
L
Monticone
G
G
Greco
A V
AV
Menzinger
G
G
Ghirlanda
G
G
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001342:Autonomic Nervous System Diseases / Q000503:physiopathology*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D003929:Diabetic Neuropathies / Q000503:physiopathology*; D006321:Heart / Q000294:innervation*; D006339:Heart Rate / Q000502:physiology; D008297:Male; D010275:Parasympathetic Nervous System / Q000503:physiopathology; D051381:Rats; D011919:Rats, Inbred Strains
D000109:Acetylcholine