Mitochondrial nucleotide translocase from skeletal muscle of halothane sensitive pigs: an electrophoretic study.
ATP translocation into mitochondria isolated from halothane-sensitive pig (HP) muscle was dramatically reduced compared with normal pigs (NP). To determine if this was due to a decreased amount of ATP translocase in the mitochondrial membranes, or a structural modification of this protein, an electrophoretic study was undertaken. Total proteins and purified translocase preparations from (NP) and (HP) mitochondria were analyzed by SDS gel electrophoresis. In the two types of mitochondria no significant differences were observed either in the amount of ATP translocase or in the molecular weight. Also, neither nonequilibrium pH gradient gel electrophoresis nor the analysis of peptides produced by limited proteolysis revealed any structural difference between the two types of protein. On the basis of these results, the depressed translocase activity observed in (HP) mitochondria cannot be explained by a reduced amount of the nucleotide translocase, nor a structural alteration of this protein. Possible inhibition of (HP) translocase activity by Ca2+ accumulation or by other mechanisms is discussed.
Ayoub
S
S
INSERM U. 195, Faculté de Medecine, Université de Clermont-Ferrand, France.
Berson
G
G
Rock
E
E
Younes
A
A
D000818:Animals; D004195:Disease Models, Animal; D006221:Halothane / Q000494:pharmacology*; D007526:Isoelectric Point; D008305:Malignant Hyperthermia / Q000235:genetics / Q000503:physiopathology*; D008931:Mitochondria, Muscle / Q000737:chemistry*; D000226:Mitochondrial ADP, ATP Translocases / Q000737:chemistry* / Q000302:isolation & purification; D008970:Molecular Weight; D010449:Peptide Mapping; D013552:Swine / Q000502:physiology*
D000226:Mitochondrial ADP, ATP Translocases; D006221:Halothane