Stimulation of DNA polymerase alpha activity by microtubule-associated proteins.
Microtubule-associated protein 2 (MAP2) isolated from porcine brains stimulated the activity of DNA polymerase alpha immunopurified from calf thymus or human lymphoma cells, in a dose-dependent manner. This stimulation was pronounced when activated DNA or poly(dA).(dT)10 was used as the template-primer. DNA polymerase alpha bound to a MAP2-immobilized column, whereas preincubation of the enzyme with unbound MAP2 prevented binding to the column. These events suggested that a physical binding occurred between the polymerase and MAP2. Kinetic analyses revealed that MAP2 decreased the Km value of the polymerase for deoxyribonucleotides, irrespective of the species of template-primer. A concomitant increase in Vmax was observed; however, the extent of the increase depended on the species of template-primer. MAP2 also decreased the Km value of the polymerase for template-primers when activated DNA of poly(dA).(dT)10 was used as the template-primer. Product analyses showed that MAP2 did not significantly alter the processivity of the polymerase and the increment of Vmax is considered to be due to an increase in the frequency of initiation of DNA synthesis. The stimulation by MAP2 occurred specifically in the activity of DNA polymerase alpha, but not DNA polymerases beta, gamma, and I from Escherichia coli. Other MAPs, tau and 190-kDa MAP, could substitute for MAP2. Thus, the specific stimulation of DNA polymerase alpha by MAPs supports the notion of a possible involvement of MAPs or MAP-like proteins in DNA replication, in vivo.
Shioda
M
M
Department of Physiological Chemistry and Nutrition, Faculty of Medicine, University of Tokyo, Japan.
Okuhara
K
K
Murofushi
H
H
Mori
A
A
Sakai
H
H
Murakami-Murofushi
K
K
Suzuki
M
M
Yoshida
S
S
D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism; D004257:DNA Polymerase II / Q000378:metabolism*; D007700:Kinetics; D008869:Microtubule-Associated Proteins / Q000378:metabolism / Q000494:pharmacology*; D011067:Poly dA-dT / Q000378:metabolism; D013379:Substrate Specificity; D013552:Swine; D013698:Templates, Genetic; D016875:tau Proteins / Q000494:pharmacology
D008869:Microtubule-Associated Proteins; D016875:tau Proteins; D011067:Poly dA-dT; D004247:DNA; D004257:DNA Polymerase II