Involvement of protein kinase activation in neurotrophic effects of basic fibroblast growth factor in cultured brain neurons.
The influences of K-252a and staurosporine, protein kinase inhibitors, on neurotrophic effects of basic fibroblast growth factor (bFGF) were investigated in dissociated cell cultures of the striatum, hippocampus and cerebellum of fetal rats. Addition of 1 ng/ml bFGF enhanced the survival of cultured neurons of all brain regions tested. Both K-252a (10-200 nM) and staurosporine (1-100 nM) blocked the survival promoting effects of bFGF in a concentration-dependent manner. These results suggest that bFGF exerts its neurotrophic effects through activation of protein kinase(s).
Abe
K
K
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Iri
Y
Y
Takayanagi
M
M
Saito
H
H
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D002227:Carbazoles / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology; D003342:Corpus Striatum / Q000166:cytology; D004789:Enzyme Activation; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology; D026121:Indole Alkaloids; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D019311:Staurosporine
D000470:Alkaloids; D002227:Carbazoles; D026121:Indole Alkaloids; D047428:Protein Kinase Inhibitors; D016222:Fibroblast Growth Factor 2; C049985:staurosporine aglycone; D011494:Protein Kinases; D019311:Staurosporine