title: Distribution of arrestin-like protein and beta-subunit of GTP-binding proteins in quail choroid plexuses.
abstract: Monoclonal antibodies (Mabs) directed against retinal arrestin (S-antigen) were used to detect and characterize this protein in choroid plexus (CP) of quails maintained during eight days, either under long-day photoperiods or in constant darkness. Immunocytochemistry and Western blotting confirmed the presence and the distribution of an arrestin-like protein in quail CP. Arrestin-like immunoreactivities in CP were compared with those obtained with Mabs to beta 36-subunit of G proteins (G beta), alpha-subunit of transducin and rhodopsin. Rhodopsin-like and transducin-like proteins could not be detected in choroidal cells, whereas intense positive reactions were observed with anti-G beta and anti-arrestin Mabs. The strongest immunoreactivities were found in choroidal ependymocytes of the lateral and IIIrd ventricles. In CP epithelial cells lining the IVth ventricle, very weak or no immunoreactivity could be detected with Mabs to arrestin, while Mab against G beta subunit always provided a positive reaction. In quails maintained in constant darkness, arrestin- and G beta-immunoreactivities of CP epithelial cells displayed changes in cellular distribution and intensity (decrease or disappearance of the immunoreactions). The strong arrestin-like immunoreaction located in the apical region of ependymocytes suggests the preferential association of the protein with choroidal microvilli and a possible role in cerebrospinal fluid production assumed by CP cells.
authors.affiliation: Laboratoire de Neurobiologie Endocrinologique, URA CNRS 1197, Université de Montpellier II, France.
authors.forename: J
authors.initials: J
authors.lastname: Oliver
authors.forename: M
authors.initials: M
authors.lastname: Mirshahi
authors.forename: S
authors.initials: S
authors.lastname: Herbute
authors.forename: S
authors.initials: S
authors.lastname: Peraldi-Roux
authors.forename: L
authors.initials: L
authors.lastname: Trebuchon
authors.forename: P
authors.initials: P
authors.lastname: Brisson
authors.forename: J P
authors.initials: JP
authors.lastname: Collin
authors.forename: J P
authors.initials: JP
authors.lastname: Faure
authors.forename: J
authors.initials: J
authors.lastname: Gabrion
mesh_terms: D000818:Animals; D000941:Antigens / Q000378:metabolism*; D019393:Arrestin; D002831:Choroid Plexus / Q000378:metabolism*; D003623:Dark Adaptation; D005136:Eye Proteins / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D008027:Light; D010870:Pineal Gland / Q000378:metabolism; D011784:Quail; D012160:Retina / Q000378:metabolism; D012243:Rhodopsin / Q000378:metabolism; D015293:Transducin / Q000378:metabolism
chemical_list: D000941:Antigens; D019393:Arrestin; D005136:Eye Proteins; D012243:Rhodopsin; D019204:GTP-Binding Proteins; D015293:Transducin