Photoduplication and fluorescence measurement in polyacrylamide gel electrophoresis.
A simple method of photoduplication of gel electrophoresis, visualized with fluorescent reagents, is described. The procedure is convenient and rapid and requires no camera or expensive equipment. Using electrophoresis duplicating paper (Kodak), positive prints suitable for documentation or publication may be obtained. With usual photographic paper, negative prints may be obtained, allowing reliable measurement by scanning. The technique may be applied to protein or nucleic acid electrophoresis.
Berson
G
G
INSERM U 195, Laboratoire Physiologie et Pharmacologie cardiovasculaires appliquées à la rythmogènèse, Faculté de Médecine, Clermont Ferrand, France.
Robert
V
V
Ayoub
S
S
D003308:Copying Processes; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D009696:Nucleic Acids / Q000032:analysis; D011506:Proteins / Q000032:analysis*
D009696:Nucleic Acids; D011506:Proteins