Determination of carotenoids in foods by high-performance liquid chromatography.
The mean values of alpha and beta-carotene of 10 fruits and vegetables from supermarkets in Cairo and Alexandria have been determined using HPLC method, and the average vitamin A activities (in retinol equivalents) calculated. Carrots, spinach and sweet potatoes were the best sources of provitamin A activity. Beta-carotene was the more prevalent carotenoid compared with alpha-carotene. An analysis of variance including all foods investigated demonstrated that there were no significant differences among either locations or times of analysis. There was no difference between the analysed values of vitamin A activity and the USDA Handbook No. 8 values.
Abdel-Kader
Z M
ZM
Biochemistry and Nutrition Department, Women's College, Ain Shams University, Cairo, Egypt.
D002338:Carotenoids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004534:Egypt; D005638:Fruit / Q000737:chemistry*; D007202:Indicators and Reagents; D012996:Solutions; D014675:Vegetables / Q000737:chemistry*; D014801:Vitamin A / Q000032:analysis; D019207:beta Carotene
D007202:Indicators and Reagents; D012996:Solutions; D019207:beta Carotene; D014801:Vitamin A; D002338:Carotenoids; C041635:alpha-carotene