title: The DNA repair gene PSO3 of Saccharomyces cerevisiae belongs to the RAD3 epistasis group.
abstract: The mutant allele pso3-1 of Saccharomyces cerevisiae confers sensitivity to treatment with UV365nm (UVA) light-activated mono- and bi-functional psoralens. When pso3-1 is combined in double mutants with selected rad and pso mutant alleles and subjected to 8-MOP + UVA treatment, epistatic interaction with regard to survival is observed with pso1, pso2, and rad3. With the same treatment the combination of pso3-1 with rad6 and rad52 leads to synergistic interaction. For the monofunctional agent 3-carbethoxypsoralen (3-CPs) the analysis of double mutants yields the same results as with the bifunctional 8-methoxypsoralen (8-MOP) with the exception of the pso1-1pso3-1 double mutant. Here we find an additive interaction, i.e., the sensitivities of both parental strains are summed in the double mutant, which indicates a different substrate specificity of the repair activity encoded by the PSO1 and PSO3 genes.
authors.affiliation: Departamento de Biofisica, Instituto de Biociencias-UFRGS, Porto Alegre, RS, Brasil.
authors.forename: M S
authors.initials: MS
authors.lastname: Benfato
authors.forename: M
authors.initials: M
authors.lastname: Brendel
authors.forename: J A
authors.initials: JA
authors.lastname: Henriques
mesh_terms: D000483:Alleles; D004249:DNA Damage; D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D004843:Epistasis, Genetic; D011564:Furocoumarins / Q000494:pharmacology; D005800:Genes, Fungal; D008730:Methoxsalen / Q000494:pharmacology; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D004271:DNA, Fungal; D011564:Furocoumarins; C017104:3-carbethoxypsoralen; D008730:Methoxsalen