title: Automated quantitative microcolumn chromatography of haemoglobin A2.
abstract: Automated cation exchange microcolumn chromatography of haemoglobins has been modified for the analysis of haemoglobin A2. It provides the quantitative data of sufficient precision and specificity for the investigation of potential heterozygotes for beta-thalassaemia. Results have been compared with an established method of electrophoresis followed by densitometry of the eluted bands.
authors.affiliation: Department of Clinical Biochemistry, Hospitals for Sick Children, London, UK.
authors.forename: R S
authors.initials: RS
authors.lastname: Ersser
authors.forename: L
authors.initials: L
authors.lastname: Blight
authors.forename: N M
authors.initials: NM
authors.lastname: Hjelm
authors.forename: K
authors.initials: K
authors.lastname: Chambers
authors.forename: D
authors.initials: D
authors.lastname: James
mesh_terms: D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D006443:Hemoglobin A2 / Q000032:analysis*; D006801:Humans; D013789:Thalassemia / Q000097:blood
chemical_list: D006443:Hemoglobin A2