Myeloma affects both the growth and function of human osteoblast-like cells.
Myeloma behaves differently to other osteolytic tumours which metastasize to bone, in that the latter usually provoke reactive bone formation in the host bone. A previous study showed that a myeloma cell line (GM1500) secreted an osteoblast-inhibiting factor(s). The present study was undertaken to determine whether other myeloma cells also secreted a factor(s) which inhibited both cell proliferation and DNA synthesis of osteoblast-like cells and whether the myeloma also affected the function of osteoblasts. The results showed that a second cell line (Karpas 707) as well as myeloma tissue taken from two patients had a similar effect. The myeloma cells did not affect total collagen or protein synthesis, and did not affect the overall degree of mineralization. A biphasic effect was seen on alkaline phosphatase activity. Thus, although the proliferation of the pre-osteoblast was affected, the synthetic functions of the osteoblasts were not.
Evans
C E
CE
University of Manchester Department of Orthopaedic Surgery, Hope Hospital, Salford, UK.
Ward
C
C
Rathour
L
L
Galasko
C B
CB
D000469:Alkaline Phosphatase / Q000378:metabolism*; D002455:Cell Division; D003470:Culture Media / Q000494:pharmacology; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D006801:Humans; D009101:Multiple Myeloma / Q000378:metabolism*; D010006:Osteoblasts / Q000201:enzymology / Q000473:pathology* / Q000502:physiology
D003470:Culture Media; D004273:DNA, Neoplasm; D000469:Alkaline Phosphatase