[Monoclonal antibody HMB-45 in diagnosis of intra-ocular melanoma].
69 eyes, which had to be enucleated because of an uveal melanoma, were investigated immunohistologically. HMB-45-antigen, which is supposed to be highly specific for melanocytic tumours, was found in 68 cases (sensitivity 99%) with a monoclonal antibody. S 100-antigen was positive in 63 cases (sensitivity 91%) using a polyclonal antibody. The addition of HMB-45-immunohistochemistry to routine histological diagnostic procedures is valuable, when a non-melanocytic uveal tumour cannot be ruled out or when the degree of melanoma-invasion has to be determined with greater accuracy. At the site of melanoma-invasion changes of cellular reactivity seem to occur, as 11 of the 69 studied tumours (16%) showed an increased HMB-45-expression in this area. HMB-45-antigen was detectable even in paraffin embedded material obtained from eyes enucleated 30 years ago.
Rohrbach
J M
JM
Abteilung I, Universitäts-Augenklinik Tübingen.
Steuhl
K P
KP
Thanos
S
S
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor / Q000032:analysis*; D002829:Choroid / Q000473:pathology; D002830:Choroid Neoplasms / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008544:Melanocytes / Q000473:pathology; D008545:Melanoma / Q000473:pathology*; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins / Q000032:analysis*; D009418:S100 Proteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D009418:S100 Proteins
Der monoklonale Antikörper HMB-45 in der Diagnostik intraokularer Melanome.