The effect of flow on the interaction of isolated megakaryocytes with subendothelial extracellular matrix.
We have previously shown that human, guinea pig, or rat megakaryocytes, incubated under static conditions on an extracellular matrix (ECM) produced by endothelial cells, readily adhered to the matrix and underwent platelet-like shape change and thromboxane A2 secretion. We have now exposed megakaryocytes to ECM in a perfusion system similar to that used to study platelets circulated over aortic subendothelium. We used a continuous flow circuit incorporating a parallel plate perfusion chamber. Megakaryocytes were isolated to high purity from guinea pig marrow by centrifugal elutriation and velocity sedimentation. The cells were introduced into the flowing medium while the surface of an ECM-coated coverslip mounted in the chamber was observed continuously by phase-contrast video microscopy for up to 18 hours. Megakaryocytes from the flowing suspension started to adhere to the ECM within seconds. Significant adhesion occurred over a range of shear rates, from 10 to 190 seconds-1, did not appear above 300 seconds-1 and was greatest at a shear rate of 60 seconds-1. Adhesion to the ECM was specific, since there was no adherence to glass coverslips, glutaraldehyde-fixed ECM-coated coverslips, or to endothelial cells cultured on ECM-coated coverslips. At low shear rates large aggregates of megakaryocytes formed on the ECM surface; these could be detached and washed away by higher shear forces. Megakaryocytes thus acquire, even before platelet formation, an adhesive capacity similar to that of platelets. In addition, a significant fraction of the adherent megakaryocytes underwent elongation and pseudopod formation similar to that seen in marrow sinusoids.
Eldor
A
A
Department of Hematology, Hadassah University Hospital, Jerusalem, Israel.
Stromberg
R R
RR
Vlodavsky
I
I
Hy-Am
E
E
Koslow
A R
AR
Friedman
L I
LI
Levine
R F
RF
D000818:Animals; D002448:Cell Adhesion; D002449:Cell Aggregation; D005109:Extracellular Matrix / Q000378:metabolism*; D005898:Glass; D006168:Guinea Pigs; D006801:Humans; D008533:Megakaryocytes / Q000378:metabolism* / Q000648:ultrastructure; D008858:Microscopy, Phase-Contrast; D010477:Perfusion; D051381:Rats; D012212:Rheology / Q000295:instrumentation; D013314:Stress, Mechanical; D014743:Videotape Recording