Comparison of glycosidase activities in epidermis, palatal epithelium and buccal epithelium.
1. beta-Glucosidase, alpha-glucosidase, beta-galactosidase and alpha-mannosidase were measured in epidermis, palatal and buccal epithelium of the pig (Sus scrofa). 2. All three epithelia contained similar alpha-mannosidase activity (1.7-3.2 nmol mg tissue-1 hr-1 at pH 4), and none contained significant alpha-glucosidase. 3. Specific activity of beta-glucosidase was high (9-13 nmol mg tissue-1 hr-1 at pH 4) in epidermis and palate, but activity was low (less than 2 nmol mg tissue-1 hr-1) in buccal epithelium. 4. Only epidermis contained a high level of beta-galactosidase (5.8 nmol mg tissue-1 hr-1). 5. Differences in glycosidase profiles may underlie differences in permeability barrier properties in these epithelia.
Chang
F
F
Dows Institute for Dental Research, University of Iowa, Iowa City 52242.
Wertz
P W
PW
Squier
C A
CA
D000818:Animals; D002610:Cheek; D004848:Epithelium / Q000201:enzymology; D006026:Glycoside Hydrolases / Q000378:metabolism*; D008361:Mannosidases / Q000378:metabolism; D009061:Mouth Mucosa / Q000201:enzymology*; D010160:Palate, Soft / Q000201:enzymology; D012756:Sheep; D012867:Skin / Q000201:enzymology*; D014018:Tissue Distribution; D000520:alpha-Glucosidases / Q000378:metabolism; D043323:alpha-Mannosidase; D001616:beta-Galactosidase / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
D006026:Glycoside Hydrolases; D008361:Mannosidases; D000520:alpha-Glucosidases; D001617:beta-Glucosidase; D001616:beta-Galactosidase; D043323:alpha-Mannosidase