Comparative tumor-initiating ability of 7H-dibenzo(c,g)carbazole and dibenz(a,j)acridine in mouse skin.
N-heterocyclic aromatics are environmentally important carcinogenic pollutants produced by incomplete combustion of organic material. 7H-Dibenzo-(c,g)carbazole (DBC), is a potent skin and systemic carcinogen, whereas dibenz(a,j)acridine (DBA), is a carcinogen with local effects. Therefore, the overall objective of these studies was to determine the initiating ability of DBC and DBA in mouse skin using an initiation-promotion protocol. Acetone-, TPA- or BaP-treated animals were used as negative and positive controls, respectively. DBC, DBA or BaP (200 nmol) dissolved in acetone was applied once to the backs of thirty shaved Hsd:(ICR)Br female mice, followed 2 weeks later with 2 micrograms of TPA in 50 microliters of acetone applied twice a week for up to 24 weeks. Skin tumors developed in 26, 17 and 27 animals, respectively. DBC plus TPA produced a significant influx of dermal macrophages similar to that seen for BaP. Initiation with BaP, DBC or DBA moderated the effect of TPA on most other dermal parameters, particularly neutrophils. These data indicate that, DBC, with apparently different activation pathways than BaP shows similar tumor initiating ability and morphological changes as BaP.
Warshawsky
D
D
Department of Environmental Health, University of Cincinnati College of Medicine, Kettering Laboratory, OH 45267-0056.
Barkley
W
W
Miller
M L
ML
LaDow
K
K
Andringa
A
A
D000096:Acetone / Q000633:toxicity; D000166:Acridines / Q000633:toxicity*; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D002227:Carbazoles / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D005260:Female; D051379:Mice; D010212:Papilloma / Q000139:chemically induced; D012867:Skin / Q000187:drug effects*; D012878:Skin Neoplasms / Q000139:chemically induced; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity
D000166:Acridines; D002227:Carbazoles; D002273:Carcinogens; C022289:dibenzacridine; D000096:Acetone; D001564:Benzo(a)pyrene; D013755:Tetradecanoylphorbol Acetate; C013478:7H-dibenzo(c,g)carbazole