Effect of bradykinin antagonists, NG-monomethyl-L-arginine and L-NG-nitro arginine on phospholipase A2 induced oedema in rat paw.
1. The rat paw oedema produced by a local injection of phospholipase A2 from Naja mocambique mocambique has been shown to be mainly driven by the liberation of serotonin and kinins. 2. Using specific bradykinin receptor antagonists we have shown that kinins are acting through B2 receptors. 3. Using endothelium-derived relaxing factor (EDRF) synthesis inhibitors NG-monomethyl-L-arginine and L-NG-nitro arginine we have tested the possible envolvement of EDRF as mediator. 4. Our work supports the view that extracellular phospholipases A2 are involved in inflammation, and suggests a role for EDRF as mediator of extravasation in this model of inflammation.
Cirino
G
G
Department of Experimental Pharmacology, University of Naples Federico II, Italy.
Cicala
C
C
Sorrentino
L
L
Regoli
D
D
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001920:Bradykinin / Q000037:antagonists & inhibitors*; D004487:Edema / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D005070:Evans Blue; D005528:Foot / Q000473:pathology; D007704:Kininogens / Q000378:metabolism; D008297:Male; D008784:Methysergide / Q000494:pharmacology; D019335:Nitroarginine; D010741:Phospholipases A; D054467:Phospholipases A2; D051381:Rats; D011919:Rats, Inbred Strains; D019323:omega-N-Methylarginine
D007704:Kininogens; D019335:Nitroarginine; D019323:omega-N-Methylarginine; D005070:Evans Blue; D001120:Arginine; D010741:Phospholipases A; D054467:Phospholipases A2; D001920:Bradykinin; D008784:Methysergide