The expression of the amyloid precursor protein (APP) is regulated by two GC-elements in the promoter.
The structure of the promoter of the human APP gene resembles that of housekeeping genes, with the presence of a GC-rich region and the lack of a canonical TATA box. Since analysis of the expression of the APP gene, especially at the transcriptional level, might reveal factors or elements, which influence amyloid formation in Alzheimer's disease, a 5' deletion analysis of the APP promoter was performed, leading to the identification of an activating DNA fragment (Ac), acting also on a heterologous promoter. DNaseI-footprint analysis revealed three protected regions on the Ac fragment. Further gene transfer experiments showed, that at least two elements, designated A and C, confer transcriptional activity in HeLa cells. Whereas the proximal element A is a 19bp long GC-rich DNA sequence, the distal element C is a GC-palindrome with the sequence 5'GCGGCGCCGC.
Pollwein
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P
Center for Molecular Biology Heidelberg (ZMBH), University of Heidelberg, FRG.
Masters
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CL
Beyreuther
K
K
D016564:Amyloid beta-Protein Precursor / Q000235:genetics*; D001483:Base Sequence; D003596:Cytosine; D004247:DNA / Q000378:metabolism; D004252:DNA Mutational Analysis; D003850:Deoxyribonuclease I / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics*; D006147:Guanine; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics
D016564:Amyloid beta-Protein Precursor; D006147:Guanine; D003596:Cytosine; D004247:DNA; D003850:Deoxyribonuclease I