Improved detection of HIV-2 DNA in clinical samples using a nested primer-based polymerase chain reaction.
A two-step polymerase chain reaction (PCR), with four double (nested) primer pairs, used for the detection of HIV-2 in clinical samples is described. With these four nested primer pairs we could detect HIV-2 DNA in 17 of 17 virus isolates and in blood mononuclear cell samples from 31 of 37 (83.7%) seropositive individuals after ethidium bromide staining of the amplified DNA. The nested primer PCR was also compared with a single primer pair-based PCR followed by hybridization. The sensitivities of the two methods were almost equal, but the nested primer PCR offered obvious technical advantages.
Grankvist
O
O
Department of Virology, University of Umeå, Sweden.
Bredberg-Rådèn
U
U
Gustafsson
A
A
Albert
J
J
Albino
P
P
Andreasson
P A
PA
Nauclér
A
A
Biberfeld
G
G
Wadell
G
G
D000293:Adolescent; D000328:Adult; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D015342:DNA Probes; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D005784:Gene Amplification; D015498:HIV-2 / Q000235:genetics*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D008251:Macaca; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D015302:Simian Immunodeficiency Virus / Q000235:genetics; D016662:Virus Integration
D015342:DNA Probes; D004279:DNA, Viral