[Mutagenesis, induced by phosphotriester analogs of oligonucleotides and directed to the cleavage site of double-spiral DNA].
The mutagenic properties of phosphotriester analogues revealed in course of interaction with linearized plasmid DNA were studied. The plasmid-based model system permitting one to test reliably the induced mutations is proposed. The efficiency of mutagenesis was shown to depend on the length of the oligonucleotide-mutagen and the genotype of the transformed Escherichia coli strain. The possible mechanisms involved in mutagenesis are discussed.
Petrenko
V A
VA
Karpenko
L I
LI
Tat'kov
S I
SI
Smirnova
O Iu
OIu
Kuz'micheva
G A
GA
Il'ichev
A A
AA
D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D004952:Esters / Q000737:chemistry*; D005798:Genes, Bacterial; D005814:Genes, Viral; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009841:Oligonucleotides / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry*
D004269:DNA, Bacterial; D004279:DNA, Viral; D004952:Esters; D009841:Oligonucleotides; D009943:Organophosphorus Compounds
Mutagenez, indutsirovannyĭ fosfotriéfirnymi analogami oligonukleotidov i napravlennyĭ na mesta razryva dvuspiral'noĭ DNK.