Detection of untreated mycobacteria by using polymerase chain reaction and specific DNA probes.
A method for specific identification of mycobacteria by using the polymerase chain reaction on organisms taken from liquid cultures, frozen suspensions, or colonies grown on Lowenstein-Jensen slants is presented. This direct detection of mycobacterial organisms has important implications for strain typing and diagnosis.
Fries
J W
JW
Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts 02115.
Patel
R J
RJ
Piessens
W F
WF
Wirth
D F
DF
D001345:Autoradiography; D015342:DNA Probes; D003937:Diagnosis, Differential; D004587:Electrophoresis, Agar Gel; D005819:Genetic Markers; D005838:Genotype; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics; D009164:Mycobacterium Infections / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction
D015342:DNA Probes; D005819:Genetic Markers