title: Quantitative study of blood-brain barrier permeability changes after experimental whole-brain radiation.
abstract: Basic mechanisms underlying the tolerance and reaction of the central nervous system to ionizing radiation are not known precisely. We investigated the possibility of a change in blood-brain barrier (BBB) function as a causative factor for early delayed whole-brain radiation-induced cerebral dysfunction. Rats were exposed to conventional fractionation (200 cGy/d, 5 d/wk; total dose, 4000 cGy). BBB changes were assessed by means of the quantitative 14C-alpha-aminoisobutyric acid technique and electron microscopy. Studies of the passage of horseradish peroxidase across the BBB permitted comparative quantitative isotopical and qualitative morphological data. Experiments were carried out 2 to 3 weeks after the completion of the radiation exposure. The transport of 14C-alpha-aminoisobutyric acid across the BBB increased significantly in cerebral cortex and cerebellar gray matter, averaging 1.3 to 1.5 times over the normal values. Electron microscopy disclosed an intense vesicular response of the cortical microvascular endothelium that occurred without the opening of the tight junctions and resulted in an intense transport of HRP across the intact endothelium. The present data indicate that moderate doses of whole-brain radiation induce well-defined changes in BBB function, which possibly are involved in the pathogenesis of radiation-induced cerebral dysfunction in humans.
authors.affiliation: Institute of Oncology, Faculty of Medicine, University of Messina, Italy.
authors.forename: D
authors.initials: D
authors.lastname: d'Avella
authors.forename: R
authors.initials: R
authors.lastname: Cicciarello
authors.forename: F
authors.initials: F
authors.lastname: Albiero
authors.forename: M
authors.initials: M
authors.lastname: Mesiti
authors.forename: M E
authors.initials: ME
authors.lastname: Gagliardi
authors.forename: E
authors.initials: E
authors.lastname: Russi
authors.forename: A
authors.initials: A
authors.lastname: d'Aquino
authors.forename: F
authors.initials: F
authors.lastname: Tomasello
authors.forename: S
authors.initials: S
authors.lastname: d'Aquino
mesh_terms: D000621:Aminoisobutyric Acids / Q000493:pharmacokinetics; D000818:Animals; D001692:Biological Transport; D001808:Blood Vessels / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D001812:Blood-Brain Barrier / Q000528:radiation effects*; D001921:Brain / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure; D002199:Capillary Permeability; D002560:Cerebrovascular Circulation; D006735:Horseradish Peroxidase; D008297:Male; D008833:Microcirculation / Q000528:radiation effects; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000621:Aminoisobutyric Acids; C100049:2-aminoisobutyric acid; D006735:Horseradish Peroxidase