[Use of liposomes for the association of foreign genetic material to spermatozoa].
The binding of liposomes of different composition with sperm cells and their effect on cell behaviour were studied. Experiments have demonstrated, that liposomes, containing positively charged phospholipids, had maximal degree of association with sperm cells. Besides, liposomes seem to be nontoxic for sperms and do not change the functional activity of the latter. Theoretically, sperm cells may serve as a natural vector for transfer of liposome-encapsulated DNA into the ovary cells of animals for obtaining transgenic animals.
Gorlova
A V
AV
Torchilin
V P
VP
D000818:Animals; D030801:Animals, Genetically Modified; D002417:Cattle; D004247:DNA / Q000008:administration & dosage*; D005260:Female; D005818:Genetic Engineering; D008081:Liposomes; D008297:Male; D010053:Ovary; D010743:Phospholipids / Q000008:administration & dosage; D013094:Spermatozoa
D008081:Liposomes; D010743:Phospholipids; D004247:DNA
Ispol'zovanie liposom dlia assotsiatsii chuzherodnogo geneticheskogo materiala so spermatozoidami.