Molecular characterisation of kappa- and lambda-carrageenan by gel permeation chromatography, light scattering, sedimentation analysis and osmometry.
Gel permeation chromatography, in conjunction with a double detection system involving a low angle laser light scattering apparatus (LALLS) and a refractive index monitoring device (RI), has been used to obtain both the molecular weight and the molecular weight distribution of sodium salts of kappa-carrageenan and lambda-carrageenan in saline solutions. The results, Mw and Mn, are in excellent agreement with independent determinations of molar mass based on static light scattering experiments, sedimentation-diffusion analysis and osmometry. The relevance of the data is discussed with respect to current problems in carrageenan research.
Slootmaekers
D
D
Department of Physical and Macromolecular Chemistry, Gorlaeus Laboratories, University of Leiden, Netherlands.
van Dijk
J A
JA
Varkevisser
F A
FA
van Treslong
C J
CJ
Reynaers
H
H
D002351:Carrageenan / Q000737:chemistry*; D002850:Chromatography, Gel; D004058:Diffusion; D008970:Molecular Weight; D009997:Osmotic Pressure; D012031:Refractometry
D002351:Carrageenan