title: Cerebral regional capillary perfusion and blood flow after carbon monoxide exposure.
abstract: Alterations in regional cerebral blood flow (rCBF) and percent perfused capillaries (indicative of functional intercapillary distance) were determined in conscious male Long-Evans rats after reducing their blood O2-carrying capacity by exposing them to 1% CO for 12 min. rCBF was determined by the iodoantipyrine method. rCBF increased from a mean of 106 +/- 8 (SE) ml.min-1.100 g-1 before CO exposure to 173 +/- 14 ml.min-1.100 g-1 after CO exposure. There was a greater flow increase (126%) in the cerebral cortex than in the lower brain stem [pons (45%), medulla (39%)]. Presence of fluorescein isothiocyanate-labeled dextran identified the perfused capillaries before and after CO exposure. The volume fraction (Vv) and number/mm2 (Na) of all capillaries (perfused and nonperfused) in a given area of brain were determined after staining for alkaline phosphatase. The percent Vv and percent Na of perfused capillaries increased uniformly (from approximately 50% to approximately 80%) in all parts of the brain after CO exposure. In the presence of tissue hypoxia with undiminished plasma PO2, the brain vasculature allowed greater flow of blood while the microvasculature adjusted to reduce the diffusion distance for O2.
authors.affiliation: Department of Physiology and Biophysics, University of Medicine and Dentistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854-5635.
authors.forename: A K
authors.initials: AK
authors.lastname: Sinha
authors.forename: J
authors.initials: J
authors.lastname: Klein
authors.forename: P
authors.initials: P
authors.lastname: Schultze
authors.forename: J
authors.initials: J
authors.lastname: Weiss
authors.forename: H R
authors.initials: HR
authors.lastname: Weiss
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001784:Blood Gas Analysis; D001933:Brain Stem / Q000098:blood supply; D002196:Capillaries / Q000503:physiopathology; D002249:Carbon Monoxide Poisoning / Q000201:enzymology / Q000503:physiopathology*; D002540:Cerebral Cortex / Q000098:blood supply; D002560:Cerebrovascular Circulation / Q000502:physiology*; D002628:Chemoreceptor Cells / Q000502:physiology; D016650:Fluorescein-5-isothiocyanate; D000860:Hypoxia / Q000503:physiopathology; D008297:Male; D008856:Microscopy, Fluorescence; D051381:Rats
chemical_list: D000469:Alkaline Phosphatase; D016650:Fluorescein-5-isothiocyanate