Expression of bacterial mercuric ion reductase in Saccharomyces cerevisiae.
The gene merA coding for bacterial mercuric ion reductase was cloned under the control of the yeast promoter for alcohol dehydrogenase I in the yeast-Escherichia coli shuttle plasmid pADH040-2 and transformed into Saccharomyces cerevisiae AH22. The resulting transformant harbored stable copies of the merA-containing hybrid plasmid, displayed a fivefold increase in the MIC of mercuric chloride, and synthesized mercuric ion reductase activity.
Rensing
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C
Institut für Pflanzenphysiologie und Mikrobiologie, Freie Universität Berlin, Germany.
Kües
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U
Stahl
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U
Nies
D H
DH
Friedrich
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B
D000426:Alcohol Dehydrogenase / Q000235:genetics; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000235:genetics; D008627:Mercuric Chloride / Q000494:pharmacology*; D010088:Oxidoreductases / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics
D008627:Mercuric Chloride; D010088:Oxidoreductases; D000426:Alcohol Dehydrogenase; C021551:mercuric reductase