Effect of vasopressin and protein kinase C inhibitors on junctional conductance in isolated heart cell pairs.
The effect of vasopressin on junctional conductance (gj) in isolated heart cell pairs was investigated. The results indicated that vasopressin (60 nM) causes a decrease in gj of 53% (S.E. +/- 5) (n = 10) within 15 sec. of its administration to the bath. The effect of vasopressin was dose-dependent and was suppressed by staurosporine (50 microM)--a potent inhibitor of protein kinase C. Inhibitors of protein kinase C by themselves increased gj. The results indicate that protein kinase C plays an important role in the control of gj in heart muscle.
De Mello
W C
WC
Department of Pharmacology, School of Medicine, University of Puerto Rico, San Juan 00936-5067.
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D004553:Electric Conductivity / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D006352:Heart Ventricles; D007365:Intercellular Junctions / Q000187:drug effects* / Q000502:physiology; D009206:Myocardium / Q000166:cytology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D051381:Rats; D015398:Signal Transduction; D019311:Staurosporine; D014667:Vasopressins / Q000494:pharmacology*
D000470:Alkaloids; D014667:Vasopressins; D011493:Protein Kinase C; D019311:Staurosporine