title: Walking, cloning, and mapping with yeast artificial chromosomes: a contig encompassing D21S13 and D21S16.
abstract: Chromosome 21 has often been used as a model system for the development of genome mapping and cloning strategies in humans. In this report methods for systematic chromosome walking, cloning, and mapping are exemplified in the construction of a 1.5-Mb yeast artificial chromosome (YAC) contig encompassing and extending 400 kb beyond each of the genetic loci D21S13 and D21S16. Isolation of insert-terminal sequences from YACs in this contig provides a set of closely spaced physical markers. These have been used to generate a long-range genomic restriction map.
authors.affiliation: Biotechnology Department, ICI Pharmaceuticals, Macclesfield, Cheshire, United Kingdom.
authors.forename: R
authors.initials: R
authors.lastname: Butler
authors.forename: D J
authors.initials: DJ
authors.lastname: Ogilvie
authors.forename: P
authors.initials: P
authors.lastname: Elvin
authors.forename: J H
authors.initials: JH
authors.lastname: Riley
authors.forename: R S
authors.initials: RS
authors.lastname: Finniear
authors.forename: G
authors.initials: G
authors.lastname: Slynn
authors.forename: J E
authors.initials: JE
authors.lastname: Morten
authors.forename: A F
authors.initials: AF
authors.lastname: Markham
authors.forename: R
authors.initials: R
authors.lastname: Anand
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D016386:Chromosome Walking; D015825:Chromosomes, Fungal; D002891:Chromosomes, Human, Pair 21; D003001:Cloning, Molecular; D004247:DNA; D015342:DNA Probes; D015226:Dinucleoside Phosphates / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D015723:Gene Library; D005819:Genetic Markers / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
chemical_list: D004247:DNA; D015342:DNA Probes; D015226:Dinucleoside Phosphates; D005819:Genetic Markers; C015772:CpG dinucleotide