Renaturation of DNA catalysed by yeast DNA repair and recombination protein RAD10.
The RAD10 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of ultraviolet-damaged DNA, and it functions in mitotic recombination. RAD10 has homology to the human excision repair gene ERCC-1. Here we describe the purification of the protein encoded by RAD10 and show that it is a DNA-binding protein with a strong preference for single-stranded DNA. We also show that RAD10 promotes the renaturation of complementary DNA strands.
Sung
P
P
Department of Biology, University of Rochester, New York 14627.
Prakash
L
L
Prakash
S
S
D004260:DNA Repair; D004271:DNA, Fungal / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D004720:Endonucleases; D005656:Fungal Proteins / Q000378:metabolism* / Q000494:pharmacology; D009695:Nucleic Acid Renaturation / Q000187:drug effects; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism
D004271:DNA, Fungal; D004277:DNA, Single-Stranded; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D004720:Endonucleases; C064162:RAD10 protein, S cerevisiae; D015719:Single-Strand Specific DNA and RNA Endonucleases