Glycoconjugates in the chinchilla tubotympanum.
Various biotinylated lectins were used to characterize and semiquantitate glycoconjugate residues in the tubotympanum. Epithelial goblet cells were stained predominantly by WGA, LFA, SNA, RCA-I, Con-A, LCA, SBA, PHA-E, and UEA; this finding suggests they contain alpha-neuraminic acid, beta-galactose, alpha-mannose, N-acetyl alpha-galactosamine, and alpha-fucose. Glandular mucous cells were stained predominantly by WGA, LFA, SNA, and RCA-I; this finding suggests that they contain alpha-neuraminic acid and beta-galactose. The glandular serous cells were stained predominantly by Con-A, WGA, and LFA; this finding suggests that they produced alpha-mannose and alpha-neuraminic acid that represented serum-type glycoprotein. The positive staining of epithelial goblet cells and glandular mucous cells with PNA after neuraminidase digestion suggests that they produced mucin-type glycoproteins. The staining of the mucous blanket by WGA, LFA, SNA, RCA-I, LCA, PNA, SBA, PHA-E, and UEA suggests the presence of alpha-neuraminic acid, beta-galactose, N-acetyl alpha-galactosamine, and alpha-fucose. The epithelial cell (nonsecretory) surface was stained largely by WGA, LFA, SNA, RCA-I, Con-A, and LCA; this finding suggests the presence of alpha-neuraminic acid, beta-galactose, and alpha-mannose.
Lim
D J
DJ
Department of Otolaryngology, Ohio State University College of Medicine, Columbus.
Coticchia
J M
JM
Ueno
K
K
Heiselman
F A
FA
Bakaletz
L O
LO
D000818:Animals; D002682:Chinchilla / Q000378:metabolism*; D004432:Ear, Middle / Q000737:chemistry*; D004848:Epithelium / Q000737:chemistry; D005064:Eustachian Tube / Q000737:chemistry*; D006001:Glycoconjugates / Q000032:analysis*; D007124:Immunoenzyme Techniques; D037102:Lectins
D006001:Glycoconjugates; D037102:Lectins