title: Comparison of automated liquid chromatographic and bioassay methods for determining spiramycin concentration in bovine plasma.
abstract: The performance of a liquid chromatographic (LC) method for spiramycin measurement in bovine plasma has been compared with that of a microbiological method. Plasma samples were obtained from cattle administered spiramycin intravenously. Comparison tests used were intraclass correlation (r1), correlation (r), and Student's paired t-test. For concentrations lower than 2.5 IU/mL, microbiological values were higher than LC values. This difference in results modified pharmacokinetic interpretation and might be explained by the presence of microbiologically active metabolites.
authors.affiliation: Ministère de l'Agriculture et de la Forêt, Centre National d'Etudes Vétérinaires et Alimentaires, La Haute Marche-Javené, Fougeres, France.
authors.forename: P
authors.initials: P
authors.lastname: Sanders
authors.forename: G
authors.initials: G
authors.lastname: Moulin
authors.forename: C
authors.initials: C
authors.lastname: Gaudiche
authors.forename: B
authors.initials: B
authors.lastname: Delepine
authors.forename: D
authors.initials: D
authors.lastname: Mourot
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000706:statistics & numerical data; D002417:Cattle / Q000097:blood*; D002853:Chromatography, Liquid / Q000706:statistics & numerical data; D012044:Regression Analysis; D012505:Sarcina / Q000187:drug effects / Q000254:growth & development; D015572:Spiramycin / Q000097:blood* / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D015572:Spiramycin