Membrane specificity of non-monotonic trends in cation-mediated injury to rapidly frozen phospholipid vesicles.
Phospholipid vesicles like erythrocyte ghosts [1] have been shown to display trends in freeze-thaw injury with Group I ions which are non-monotonic in nature. That is to say, the relative extent of injury with such ions, measured as calcein release, does not follow a lyotropic series related solely to the hydrated ionic radii of these ions. By incorporation of cholesterol into the vesicles the non-monotonic nature of these trends has been shown to be highly membrane specific. Thus the non-monotonic trends in cation mediated freeze-thaw injury are shown to be independent of bulk solution properties.
Brearley
C A
CA
Department of Pharmacy, Brighton Polytechnic, Moulsecoomb, U.K.
Hodges
N A
NA
Oliff
C J
CJ
D002412:Cations; D005452:Fluoresceins; D005615:Freezing; D008567:Membranes, Artificial; D010743:Phospholipids / Q000737:chemistry*
D002412:Cations; D005452:Fluoresceins; D008567:Membranes, Artificial; D010743:Phospholipids; C007740:fluorexon