A high performance liquid chromatographic method for the quantitation of flecainide in plasma.
A routine high performance liquid chromatographic method for the rapid determination of flecaïnide (Flecaine), using a novel internal standard, N-methylflecaïnide, has been developed. After deproteinization of spiked samples, flecaïnide was totally recovered at neutral pH. Flecaïnide and the internal standard were separated on a reversed phase XL 3 microns ODS column using 10 mM phosphate buffer, pH 3.0: acetonitrile (70:30) as mobile phase, in less than 10 min. With spectrofluorometric detection, the limit of quantitation for flecaïnide was 10 ng/mL. Intra- and inter-assay precision variations were 0.24% and 1.4%.
Broly
F
F
Laboratoire de Biochimie, Hôpital Calmette, Lille, France.
Marecaux
P
P
Houdret
N
N
Lhermitte
M
M
Houssin
R
R
D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D005424:Flecainide / Q000097:blood*; D006801:Humans
D005424:Flecainide