title: Using PCR to extend the limit of oligonucleotide synthesis.
abstract: A method has been developed to allow one to extend the practical limit of oligonucleotide synthesis by coupling the synthesis reaction to a subsequent PCR. Given that DNA synthesizers are capable of producing reasonable yields of oligonucleotides that are 125-150 bases in length, this method could be used to recover the minute amount of full-length product present in mixtures extended well beyond the established limits. This technology could be applied to gene synthesis and mutagenesis.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024.
authors.forename: M L
authors.initials: ML
authors.lastname: Michaels
authors.forename: H M
authors.initials: HM
authors.lastname: Hsiao
authors.forename: J H
authors.initials: JH
authors.lastname: Miller
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D016133:Polymerase Chain Reaction; D019914:Taq Polymerase; D013698:Templates, Genetic
chemical_list: D009841:Oligonucleotides; D004247:DNA; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase