Pigment cell localizations in anuran ventral skin at climactic metamorphosis.
In anuran amphibians, the specific color pattern of the skin is expressed after metamorphosis, and its formation involves pigment cell migrations. Pigment cells are differently distributed in the tadpole, larval, and froglet skin. To learn more about their fate during metamorphic climax and in the young froglet, we focused our attention on the different localizations of larval melanophores and iridophores in the ventral skin of Rana esculenta before and during skin homing. Localizations of melanophores and iridophores can be elucidated at the developmental stages suggested by Taylor and Kollros (TK stages). At TK stage II (during early premetamorphosis), large melanophores beneath the larval skin are detected. At TK stage X, dispersed melanophores lie under bundles of muscular striated fibrils near the larval skin; they are also observed at the vascular level. At TK stage XVII (prometamorphosis), melanophores are extended on the inner side of the basement lamellar collagen. At the end of prometamorphosis, iridophores are located with melanophores in the separating space between attached basement collagen and derived basement collagen. At TK stage XX (earlier climax), melanophores and iridophores are detected inside the upper extremities of fractures opened in the derived basement collagen. At TK stage XXIV (later climax), both types of larval pigment cells are observed in the inner extremities of breaks derived from the fractures. During climax, these pigment cells occupy the well-formed breaks. At TK stage XXV in young froglet, the pigment cells remain alone in the breaks formed in the derived basement collagen. Briefly, breaks in the basement lamellar collagen are opened by invading cell processes of mesenchymal cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Denèfle
J P
JP
Centre de Biologie Cellulaire, CNRS, Ivry-sur-Seine, France.
Lechaire
J P
JP
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism / Q000648:ultrastructure; D002465:Cell Movement; D003094:Collagen / Q000378:metabolism; D008675:Metamorphosis, Biological; D010858:Pigmentation / Q000502:physiology*; D011893:Rana esculenta / Q000254:growth & development*; D012867:Skin / Q000166:cytology / Q000254:growth & development*; D012879:Skin Physiological Phenomena
D003094:Collagen