A quantitative study of the pulsatile parameters of CRH-41 secretion in unanesthetized free-moving rats.
Having recently improved the sensitivity of the RIA CRH-41 measurements in perfusates from push-pull cannulas implanted in the rat median eminence, we explored quantitatively the pulsatile parameters of the CRH-41 measured with this technique at 2.5 or 5 min intervals in a series of unanesthetized male rats under basal conditions. The data were analysed by computer using 4 algorithms, i.e. Santen & Bardin, Ultra, Pulsar and Periodogram. Under a basal mean secretion rate of 15.5 pg CRH-41/15 min, the pulsatile pattern of CRH-41 release was statistically assessed with the 4 methods, with a mean pulse frequency of 3.1 c/h, pulse length of 11.6 min and a peak amplitude above mean levels of 4 pg. The respective advantages of the 4 algorithms and the physiological relevance of the neurohormone's pulsatility are discussed.
Ixart
G
G
Endocrinological Neurobiology Laboratory, URA 1197-CNRS, University of Montpellier II, France.
Barbanel
G
G
Nouguier-Soulé
J
J
Assenmacher
I
I
D000204:Activity Cycles; D000465:Algorithms; D000704:Analysis of Variance; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000032:analysis / Q000378:metabolism*; D008297:Male; D008473:Median Eminence / Q000502:physiology*; D009043:Motor Activity; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains
D003346:Corticotropin-Releasing Hormone