Toxicity of gentamicin in organ culture of fetal rat intestine.
The effect of gentamicin in the culture of fetal rat intestine was studied. Fetal rat intestine was cultured with gentamicin or kanamycin at the concentration between 4 to 200 micrograms/ml. Kanamycin did not have influence on lactase, maltase and ALP activities. On the other hand, gentamicin caused decrease of lactase and ALP activities at the concentration of 40 and 200 micrograms/ml compared with the activities at 4 micrograms/ml. Maltase activities did not decrease with gentamicin. Our data suggest that gentamicin could affect lactase and ALP activities and lower concentration should be used in the culture.
Shimizu
K
K
Department of Pediatrics, Osaka University Medical School, Japan.
Harada
T
T
Okada
S
S
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005839:Gentamicins / Q000633:toxicity*; D007583:Jejunum / Q000187:drug effects* / Q000196:embryology / Q000201:enzymology; D007612:Kanamycin / Q000494:pharmacology; D043322:Lactase; D009924:Organ Culture Techniques; D051381:Rats; D011919:Rats, Inbred Strains / Q000196:embryology; D000520:alpha-Glucosidases / Q000032:analysis; D001616:beta-Galactosidase / Q000032:analysis
D005839:Gentamicins; D007612:Kanamycin; D000469:Alkaline Phosphatase; D043322:Lactase; D000520:alpha-Glucosidases; D001616:beta-Galactosidase