title: A functional and structural analysis of the Sex combs reduced locus of Drosophila melanogaster.
abstract: We have undertaken a developmental genetic analysis of the homeotic gene Sex combs reduced (Scr) of Drosophila melanogaster by examining embryonic and adult phenotypes of mutations affecting Scr gene function. Molecular mapping of Scr breakpoint lesions has defined a segment of greater than 70 kb of DNA necessary for proper Scr gene function. This region is split by the fushi tarazu (ftz) gene, with lesions affecting embryonic Scr function molecularly mapping to the region proximal (5') to ftz and those exhibiting polyphasic semilethality predominantly mapping distal (3') to ftz. Gain-of-function mutations are associated with genomic rearrangements and map throughout the Scr locus. Our analysis has revealed that the Scr locus encompasses genetic elements that are responsible for functions in both the embryonic and larval to adult periods of development. From these studies, we conclude that Scr is a complex genetic locus with an extensive regulatory region that directs functions required for normal head and thoracic development in both the embryo and the adult and that the regulation of Scr during these two periods is distinct.
authors.affiliation: Howard Hughes Medical Institute, Indiana University, Bloomington 47405.
authors.forename: A M
authors.initials: AM
authors.lastname: Pattatucci
authors.forename: D C
authors.initials: DC
authors.lastname: Otteson
authors.forename: T C
authors.initials: TC
authors.lastname: Kaufman
mesh_terms: D000483:Alleles; D000818:Animals; D003433:Crosses, Genetic; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D005260:Female; D005786:Gene Expression Regulation; D005804:Genes, Lethal; D005816:Genetic Complementation Test; D007301:Insect Hormones / Q000235:genetics*; D008297:Male; D009154:Mutation; D010641:Phenotype; D011995:Recombination, Genetic; D015183:Restriction Mapping; D013696:Temperature; D014157:Transcription Factors
chemical_list: D029721:Drosophila Proteins; D007301:Insect Hormones; C059627:Scr protein, Drosophila; D014157:Transcription Factors