Hepatocytes enhance neurite regeneration and survival from transected nerve terminals.
The culture of hepatocytes dissociated from adult mice by the collagenase perfusion method enables neurons or neuronal tissues to be cultured with hepatocytes or in hepatocyte-conditioned media. Co-culture with hepatocytes or hepatocyte-conditioned media enhances neurite regeneration and their survival from nerve-transected terminals of dorsal root ganglia with nerve fibers dissected from adult and aged mice. Hepatocytes secrete a factor which enhances not only neurite regeneration but also neurite survival. Activities of other known neurotrophic factors were not as crucial as those of the hepatocyte-conditioned medium, suggesting that this factor may differ from other trophic ones.
Horie
H
H
Department of Physiology, School of Medicine, Yokohama City University, Japan.
Fukuda
N
N
Bando
Y
Y
D000367:Age Factors; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000648:ultrastructure; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006133:Growth Substances / Q000378:metabolism / Q000494:pharmacology*; D006360:Heat-Shock Proteins / Q000378:metabolism / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000494:pharmacology; D009416:Nerve Regeneration / Q000187:drug effects; D009924:Organ Culture Techniques; D013002:Somatomedins / Q000494:pharmacology
D003470:Culture Media; D006133:Growth Substances; D006360:Heat-Shock Proteins; D007328:Insulin; D009414:Nerve Growth Factors; D013002:Somatomedins; D016222:Fibroblast Growth Factor 2