Enzymic N-demethylation reaction catalysed by red blood cell cytosol.
Red blood cell cytosol promotes enzymic N-demethylation reactions which display typical Michaelis-Menten kinetics with respect to N-methylaniline as substrate. The demethylase activity is linked with hemoglobin (Hb) and is enhanced in the presence of NADH and the NADH-methemoglobin reductase system. It has been adduced that Hb in its oxygenated form is involved in the reaction.
Stecca
C
C
Laboratory of Teratogenesis and Mutagenesis, Catholic University of Louvain, Brussels, Belgium.
Cumps
J
J
Duverger-Van Bogaert
M
M
D000814:Aniline Compounds / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D003600:Cytosol / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D009243:NAD / Q000378:metabolism / Q000494:pharmacology; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism*; D010108:Oxyhemoglobins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000814:Aniline Compounds; D010108:Oxyhemoglobins; D009243:NAD; D010089:Oxidoreductases, N-Demethylating; C021313:methylaniline