An efficient, sequence-specific method for crosslinking complementary oligonucleotides using binuclear platinum complexes.
The binuclear PtII complexes [(trans-Pt(NH3)2Cl)2 (NH2(CH2)nNH2)]Cl2 (n = 4, 5 or 6), crosslink oligodeoxynucleotide-5'-phosphorothioates rapidly, specifically and efficiently to complementary single-stranded oligodeoxynucleotide targets. In the case that we investigated in detail, the most abundant crosslink is formed to the G residue complementary to the 5'-terminal C residue of the phosphorothioate. Less efficient crosslinking occurs to many other residues of the target. The same PtII complexes also bring about crosslinking efficiently to the polypurine tract in triple-helical DNA.
Gruff
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Salk Institute for Biological Studies, San Diego, CA 92186-5800.
Orgel
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D001482:Base Composition; D001483:Base Sequence; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004586:Electrophoresis; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*
D003432:Cross-Linking Reagents; D009838:Oligodeoxyribonucleotides; D010984:Platinum