title: Prostaglandin E2 receptor of rat submandibular salivary glands.
abstract: The binding characteristics of the PGE2 receptor were investigated in membrane preparations from these glands. Specific [3H]PGE2 binding was linear as a function of the membrane protein concentration and reached steady state by 40 min of incubation at 37 degrees C under neutral pH. Scatchard analysis of the binding data produced a curvilinear plot with a Kd of 0.18 nM and Bmax of 1.02 fmol/mg protein for the high-affinity binding sites, and a Kd of 181 nM and Bmax of 5.72 pmol/mg protein for the low-affinity binding sites. A competitive displacement study indicated that the receptor was specific for prostaglandins of the E series. The study is the first to demonstrate the presence of the PGE2 receptor in rat submandibular gland and to provide its biochemical features.
authors.affiliation: Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
authors.forename: C Y
authors.initials: CY
authors.lastname: Wu-Wang
authors.forename: S L
authors.initials: SL
authors.lastname: Wang
authors.forename: P
authors.initials: P
authors.lastname: Yao
authors.forename: A
authors.initials: A
authors.lastname: Slomiany
authors.forename: B L
authors.initials: BL
authors.lastname: Slomiany
mesh_terms: D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D005069:Evaluation Studies as Topic; D006863:Hydrogen-Ion Concentration; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry*; D011458:Prostaglandins E; D051381:Rats; D011919:Rats, Inbred Strains; D011982:Receptors, Prostaglandin / Q000737:chemistry*; D012469:Salivary Glands / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
chemical_list: D008565:Membrane Proteins; D011458:Prostaglandins E; D011982:Receptors, Prostaglandin