title: Analysis of trypsin inhibitors and lectins in white kidney beans (Phaseolus vulgaris, var. Processor) in a combined method.
abstract: Buffered saline extraction, affinity chromatography, and Folin-BSA protein assay were used consecutively to provide a combined method for analysis of trypsin inhibitors and lectins in white kidney beans (Phaseolus vulgaris, var. Processor). The method was tested by following the decrease of both antinutritional factors by germination of the beans for 7 days at 20 degrees C. Repeatability coefficients of variation were 2-7.4% for the trypsin inhibitors and 2.2-10% for the lectins. After 7 days of germination, trypsin inhibitors and lectins were reduced by 72 and 92%, respectively.
authors.affiliation: Agricultural University, Department of Food Science, Wageningen, The Netherlands.
authors.forename: J P
authors.initials: JP
authors.lastname: Roozen
authors.forename: J
authors.initials: J
authors.lastname: de Groot
mesh_terms: D002846:Chromatography, Affinity / Q000706:statistics & numerical data; D007887:Fabaceae / Q000737:chemistry*; D007202:Indicators and Reagents; D037102:Lectins / Q000032:analysis*; D008982:Molybdenum; D037121:Plant Lectins; D010946:Plants, Medicinal; D012710:Serum Albumin, Bovine; D014357:Trypsin / Q000032:analysis*; D014414:Tungsten; D017973:Tungsten Compounds
chemical_list: C029556:Folin's phenol reagent; D007202:Indicators and Reagents; D037102:Lectins; D037121:Plant Lectins; D017973:Tungsten Compounds; D012710:Serum Albumin, Bovine; D008982:Molybdenum; D014357:Trypsin; D014414:Tungsten