Cloning and sequencing a cDNA encoding human ribosomal protein S25.
A full-length cDNA clone has been isolated from a cDNA library prepared from mRNA of adriamycin-resistant human leukemia HL60 cells. The nucleotide sequence of this cDNA has been determined and the protein coded for by the gene identified. The cDNA encodes a polypeptide of 125 amino acids (aa) with a deduced Mr of 13750. The deduced aa sequence of this protein has 56% homology to yeast ribosomal protein S31. Western-blot analysis using antibodies directed against a synthetic peptide based on the deduced aa sequence identifies the gene product as the human ribosomal protein S25.
Li
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Center for Basic Cancer Research, Division of Biology, Kansas State University, Manhattan 66506.
Latoud
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Center
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MS
D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D004317:Doxorubicin / Q000494:pharmacology; D004351:Drug Resistance / Q000235:genetics; D006801:Humans; D007938:Leukemia / Q000235:genetics; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
C489168:RPS25 protein, human; D012269:Ribosomal Proteins; D004317:Doxorubicin