Effects of the adenylate cyclase activator forskolin and its inactive derivative 1,9-dideoxyforskolin on insect cytochrome P-450 dependent steroid hydroxylase activity.
The adenylate cyclase activator forskolin and its pharmacologically inactive derivative 1,9-dideoxyforskolin were found to inhibit in a dose-dependent fashion the ecdysone 20-monooxygenase activity associated with wandering stage larvae of Drosophila melanogaster and fat body and midgut from last instar larvae of the tobacco hornworm, Manduca sexta. The concentrations of these labdane diterpenes required to elicit a 50% inhibition of the cytochrome P-450 dependent steroid hydroxylase activity in the insect tissues ranged from approximately 5 x 10(-6) to 5 x 10(-4) M.
Keogh
D P
DP
Department of Biological Sciences, Bowling Green State University, Ohio 43403-0212.
Mitchell
M J
MJ
Crooks
J R
JR
Smith
S L
SL
D000262:Adenylyl Cyclases / Q000187:drug effects*; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D005576:Colforsin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000502:physiology*; D004331:Drosophila melanogaster / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects; D007814:Larva / Q000201:enzymology; D009036:Moths / Q000201:enzymology; D013250:Steroid Hydroxylases / Q000187:drug effects*
D005576:Colforsin; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; C020095:ecdysone 20-hydroxylase; D000262:Adenylyl Cyclases; C047983:1,9-dideoxyforskolin