title: 31P-NMR assignment and conformational study of NADPH bound to Lactobacillus casei dihydrofolate reductase based on two-dimensional 1H-31P-heteronuclear and 1H-detected 1H-31P-shift-correlation experiments.
abstract: For any detailed NMR conformational study of a protein-ligand complex it is essential to have specific resonance assignments. We have now assigned the pyrophosphate 31P resonances in spectra of NADPH bound to Lactobacillus casei dihydrofolate reductase (DHFR) by using a combination of 1H-31P-heteronuclear shift-correlation (HETCOR), 1H-31P-heteronuclear multiple-quantum-coherence correlation spectroscopy (HMQC-COSY), 1H-1H COSY, homonuclear Hartmann-Hahn (HOHAHA) and NOE spectroscopy (NOESY) experiments. The nicotinamide pyrophosphate phosphorus, P(n), has been unequivocally assigned to a signal (-14.07 ppm) which shows a large 3JP-O-C-H coupling constant. Such a coupling constant when combined with the appropriate Karplus relationship provides conformational information about the P-O-C-H torsion angle. The torsion angle changes by 65 degrees +/- 10 degrees for the binary complex compared with the value in free NADPH. The observed coupling constants for the binary (DHFR--NADPH) and ternary (DHFR--NADPH--methotrexate) complexes (12.3 and 10.5 +/- 0.6 Hz, respectively) indicate that the pyrophosphate group has a similar conformation in the two complexes.
authors.affiliation: Laboratory of Molecular Structure, National Institute for Medical Research, Mill Hill, London, England.
authors.forename: I P
authors.initials: IP
authors.lastname: Gerothanassis
authors.forename: B
authors.initials: B
authors.lastname: Birdsall
authors.forename: C J
authors.initials: CJ
authors.lastname: Bauer
authors.forename: J
authors.initials: J
authors.lastname: Feeney
mesh_terms: D007780:Lacticaseibacillus casei / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D008727:Methotrexate / Q000378:metabolism; D008968:Molecular Conformation; D009249:NADP / Q000737:chemistry / Q000378:metabolism*; D010710:Phosphates / Q000737:chemistry; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry / Q000378:metabolism*
chemical_list: D010710:Phosphates; D009249:NADP; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate