Molecular cloning of the gene encoding the bovine brain ribonuclease and its expression in different regions of the brain.
In this paper we report the molecular cloning of the gene encoding the bovine brain ribonuclease. The nucleotide sequence determined in this work shows a high degree of identity to the homologous gene encoding the bovine pancreatic ribonuclease. Processing of the primary transcripts of these genes also follows a similar pathway, splicing of the unique intron in the 5' untranslated region occurs at corresponding positions. Expression of the bovine brain ribonuclease gene can be detected both at the transcriptional and translational levels in all the regions of the brain examined.
Sasso
M P
MP
Dipartimento di Chimica Organica e Biologica, Università di Napoli, Italy.
Carsana
A
A
Confalone
E
E
Cosi
C
C
Sorrentino
S
S
Viola
M
M
Palmieri
M
M
Russo
E
E
Furia
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology* / Q000378:metabolism; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012260:Ribonucleases / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D014158:Transcription, Genetic
D004247:DNA; D012260:Ribonucleases