Lignin peroxidase from the basidiomycete Phanerochaete chrysosporium is synthesized as a preproenzyme.
The cDNA clone L18 encoding lignin peroxidase LiP2, the most highly expressed LiP isozyme from Phanerochaete chrysosporium strain OGC101, was isolated and sequenced. Comparison of the cDNA sequence with the N-terminal sequence of the mature LiP2 protein isolated from culture medium suggests that the mature protein contains 343 amino acids (aa) and is preceded by a 28-aa leader sequence. In vitro transcription followed by in vitro translation and processing by signal peptidase resulted in cleavage at a site following the Ala21 (counted from the N-terminal Met1 of the initial translation product). The resultant protein contains a 7-aa propeptide, indicating that LiP is synthesized as a preproenzyme.
Ritch
T G
TG
Department of Chemical and Biological Sciences, Oregon Graduate Institute for Science and Technology, Beaverton 97006-1999.
Nipper
V J
VJ
Akileswaran
L
L
Smith
A J
AJ
Pribnow
D G
DG
Gold
M H
MH
D000595:Amino Acid Sequence; D001483:Base Sequence; D001487:Basidiomycota / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D004792:Enzyme Precursors / Q000235:genetics / Q000378:metabolism*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D014176:Protein Biosynthesis / Q000235:genetics; D011499:Protein Processing, Post-Translational / Q000502:physiology; D021382:Protein Sorting Signals / Q000235:genetics / Q000378:metabolism
D004792:Enzyme Precursors; D007527:Isoenzymes; D021382:Protein Sorting Signals; D010544:Peroxidases; C042858:lignin peroxidase