Changes in tracheal mucosal thickness and blood flow in sheep.
Airway narrowing may be produced by increasing the blood volume of the airway mucosa. Here changes in tracheal mucosal thickness (MTtr) were measured in 10 anesthetized sheep. Arteries to the cervical trachea were isolated, and blood flow (Qtr) was measured with an electromagnetic flow probe. Simultaneous changes in MTtr were measured with a mechanical probe over a fixed cartilage. Arterial injections of phenylephrine produced dose-related falls in Qtr and MTtr with a maximum peak fall in MTtr of -104 +/- 18 (SE) microns. Methacholine, bradykinin, albuterol, and histamine produced dose-related increases in Qtr. The largest peak increase in MTtr of 308 +/- 121 microns was seen with bradykinin. For methacholine, albuterol, and histamine the largest increases in MTtr were 154 +/- 47, 45 +/- 10, and 153 +/- 31 microns, respectively. The increases in MTtr were not always closely dose related. The peak changes in MTtr occurred substantially later than those in Qtr for all the drugs and up to 120 s later for methacholine and bradykinin. Generally, changes in MTtr and Qtr persisted for less than 10 min; at the higher doses of bradykinin increases in MTtr lasted for up to 15 min. Changes in MTtr were most closely associated in time with changes in Qtr for the vasoconstrictor phenylephrine. These changes in MTtr would alter airway resistance little in the normal trachea and by substantially more in smaller airways such as the bronchi or in the narrowed trachea. Changes in mucosal thickness may be due not only to changes in tracheal blood volume but may also reflect the effects of tissue edema and mucus secretion.
Corfield
D R
DR
Department of Physiology, St. George's Hospital Medical School, London, United Kindom.
Hanafi
Z
Z
Webber
S E
SE
Widdicombe
J G
JG
D000403:Airway Resistance / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005260:Female; D009092:Mucous Membrane / Q000187:drug effects / Q000502:physiology; D009119:Muscle Contraction / Q000502:physiology; D012039:Regional Blood Flow / Q000187:drug effects / Q000502:physiology; D012756:Sheep; D014132:Trachea / Q000033:anatomy & histology* / Q000098:blood supply / Q000187:drug effects