title: Exercise-induced muscle damage in the rat: the effect of vitamin E deficiency.
abstract: Rats, fed a vitamin-E-deficient diet for 6 weeks, performed treadmill exercise for 2 h. Muscle damage was assessed by measuring the creatine kinase (CK) activity in plasma before and after exercise, and by studying semi-thin longitudinal sections of the soleus muscle 48 h after running. Vitamin-E-deficient male and female rats showed an increased post-exercise CK activity when compared to matched controls, but male rats showed a larger CK response than females. This rise in plasma CK activity was caused mainly by an increased activity of the muscle-specific CK-isoenzyme, CK-MM (males + 1238%; females + 540%, P less than 0.05). In a parallel histological study we observed in vitamin-E-deficient male rats a dramatic and significant disturbance of the normal cyto-architecture of the muscle fibres after exercise (focal necrosis, phagocytosis and cellular infiltrates), whereas in females only minor, non-significant, changes were seen. We conclude that vitamin E deficiency enhances the susceptibility to exercise-induced muscle damage in male rats more than in female rats. This difference between the sexes is attributed to the protective effect of oestradiol that remains operative in female rats when the vitamin E status is disturbed: male rats lack such hormonal protection.
authors.affiliation: Department of Neurology, University Hospital Utrecht, The Netherlands.
authors.forename: G J
authors.initials: GJ
authors.lastname: Amelink
authors.forename: W A
authors.initials: WA
authors.lastname: van der Wal
authors.forename: J H
authors.initials: JH
authors.lastname: Wokke
authors.forename: B S
authors.initials: BS
authors.lastname: van Asbeck
authors.forename: P R
authors.initials: PR
authors.lastname: Bär
mesh_terms: D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism; D008297:Male; D009043:Motor Activity / Q000502:physiology*; D009132:Muscles / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012727:Sex Characteristics; D014811:Vitamin E Deficiency / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology*
chemical_list: D003402:Creatine Kinase