Binding of Cu(2+)-beta-cyclodextrin complex to calf thymus DNA.
Cu(2+)-beta-cyclodextrin (1:1) complex has been found by UV, fluorescence and CD spectroscopy, polarimetry and gel electrophoresis to bind reversibly to calf thymus DNA. Using UV the binding constant was found to be 45280 +/- 7100 M-1. The binding of the complex Cu(2+)-BCD with DNA was stronger than that of free Cu2+. However the ternary complex formed thus was destroyed by EDTA.
Divakar
S
S
Organic Chemistry Section, Central Food Technological Research Institute, Mysore, India.
Ramasarma
P R
PR
Maheswaran
M M
MM
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D003300:Copper / Q000378:metabolism; D003505:Cyclodextrins / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004492:Edetic Acid; D007700:Kinetics; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D047392:beta-Cyclodextrins; D003300:Copper; D004247:DNA; D004492:Edetic Acid; C031215:betadex