Production of monoclonal antibodies against human 6-pyruvoyl tetrahydropterin synthase and immunocytochemical localization of the enzyme.
Monoclonal antibodies were produced against human pituitary gland 6-pyruvoyl tetrahydropterin synthase, one of the key enzymes in the biosynthesis of tetrahydrobiopterin, by in vitro immunization with the antigen directly blotted from SDS-PAGE to polyvinylidene difluoride membranes. The antibodies produced show crossreactivity in the enzyme linked immunosorbent assay, not only with the human 6-pyruvoyl tetrahydropterin synthase but some also with the same enzyme isolated from salmon liver. 6-Pyruvoyl tetrahydropterin synthase was localized immuno-enzymatically in peripheral blood smears and in skin fibroblasts by the use of these monoclonal antibodies and the alkaline phosphatase monoclonal anti-alkaline phosphatase labeling technique.
Guzman
J
J
Department of Pediatrics, University of Zürich, Switzerland.
Schoedon
G
G
Blau
N
N
D000429:Alcohol Oxidoreductases / Q000032:analysis* / Q000097:blood / Q000276:immunology; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000166:cytology / Q000201:enzymology; D005347:Fibroblasts / Q000201:enzymology; D006098:Granulocytes / Q000166:cytology / Q000201:enzymology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D019761:Phosphorus-Oxygen Lyases; D010902:Pituitary Gland / Q000201:enzymology; D012867:Skin / Q000201:enzymology*; D013601:T-Lymphocytes / Q000166:cytology / Q000201:enzymology
D000911:Antibodies, Monoclonal; D000429:Alcohol Oxidoreductases; D019761:Phosphorus-Oxygen Lyases; C043497:6-pyruvoyltetrahydropterin synthase