title: Fatty acids of healthy and periodontally diseased root substance in human teeth.
abstract: In this comparative study, samples of root substance from healthy and periodontally diseased human teeth were analyzed for fatty acids by means of a gas-chromatographic technique. The sample preparation procedure allowed specimens from individual teeth to be analyzed. The content of the fatty acids C16:0, C16:1, C18:0, and C18:1 in the superficial layer of the periodontally diseased teeth was significantly higher than that of healthy teeth. In the inner layer, there was no such difference. Four different 3-hydroxy fatty acids (3-OH C14:0, 3-OH C15:0, 3-OH C16:0, and 3-OH C17:0) were identified by combined gas-chromatography/mass-spectrometry in the tooth substance of periodontally diseased teeth. This indicates the presence of lipid A, which is the toxic component of bacterial lipopolysaccharides.
authors.affiliation: Department of Dental Materials, School of Dentistry, Bergen, Norway.
authors.forename: H
authors.initials: H
authors.lastname: Lygre
authors.forename: E
authors.initials: E
authors.lastname: Solheim
authors.forename: N R
authors.initials: NR
authors.lastname: Gjerdet
authors.forename: N
authors.initials: N
authors.lastname: Skaug
mesh_terms: D002849:Chromatography, Gas; D003739:Dental Cementum / Q000737:chemistry; D003804:Dentin / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis*; D006801:Humans; D008050:Lipid A / Q000032:analysis*; D013058:Mass Spectrometry; D010510:Periodontal Diseases / Q000378:metabolism* / Q000382:microbiology; D014092:Tooth Root / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D008050:Lipid A