The role of calcium in neutrophil migration: the effect of calcium and calcium-antagonists in electroporated neutrophils.
Chemotaxis by electroporated rabbit peritoneal neutrophils in the absence of Ca2+ is only slightly different from that in the presence of Ca2+. Pretreatment of neutrophils with quin2-AM causes inhibition of chemotaxis. Calcium antagonists as nitrendipine and verapamil are inhibitory in nanomolar concentrations, while 10(5) times higher concentrations are required for inhibition of chemotaxis by neutrophils which were not electroporated. The results support the hypothesis that Ca2+ from Ca(2+)-storing organelles is of importance for chemotaxis, but that chemotaxis is not dependent on changes in cytoplasmic Ca2+ concentrations.
Elferink
J G
JG
Department of Medical Biochemistry, University of Leiden, The Netherlands.
Boonen
G J
GJ
de Koster
B M
BM
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000097:blood / Q000494:pharmacology*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002364:Caseins / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004533:Egtazic Acid / Q000494:pharmacology; D004558:Electric Stimulation; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D009543:Nifedipine / Q000494:pharmacology; D009568:Nitrendipine / Q000494:pharmacology; D011299:Prenylamine / Q000494:pharmacology; D011817:Rabbits; D014700:Verapamil / Q000494:pharmacology
D002121:Calcium Channel Blockers; D002364:Caseins; D005456:Fluorescent Dyes; D000001:Calcimycin; D004533:Egtazic Acid; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009568:Nitrendipine; D014700:Verapamil; D008274:Magnesium; D009543:Nifedipine; D011299:Prenylamine; D002118:Calcium