title: Appearance of lectin binding affinity to the zona pellucida during hamster oocyte maturation.
abstract: In our previous study, a drastic change in terminal saccharides of glycoconjugates of the hamster zona pellucida associated with oocyte maturation was observed using light microscopic methods of lectin cytochemistry. To understand the mechanism of this change, in the present study, the correlation between the cytochemical appearance of saccharide residues in the zona pellucida and nuclear maturation was examined. Immature hamsters were treated with PMSG and hCG to induce follicular development and ovulation. The animals were euthanized 0 to 26 hrs. after the injection of PMSG or 0,1,2,3,4,5,7,9 or 11 hrs. after the injection of hCG, and ovaries were dissected out, fixed, paraffin embedded and sectioned serially. Every other paraffin section was stained with hematoxylin to observe the status of nuclei and to classify follicular growth and only the fully developed preovulatory follicles were examined in experiments. The peroxidase-labelled lectin-diaminobenzidine procedure was applied to sections. The lectins employed were WGA, SBA, MPA, UEA-I, LotusA and AAA. Germinal vesicle breakdown was observed within 3 hrs. after the administration of hCG. A positive reaction of WGA, SBA or MPA for zonae pellucidae in the fully developed preovulatory follicles appeared 1 hr. after hCG injection, and remained so for the next 10 hrs. UEA-I, Lotus A and AAA reactions were negative for all of the zonae pellucidae observed. The data indicate that the synthesis of saccharide residues such as GlcNAc and GalNAc forming zona components in the follicles is not triggered by germinal vesicle breakdown.
authors.affiliation: Department of Veterinary Anatomy, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: A
authors.initials: A
authors.lastname: Tsukise
authors.forename: M
authors.initials: M
authors.lastname: Okano
mesh_terms: D000818:Animals; D001665:Binding Sites; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D006224:Cricetinae; D005260:Female; D006064:Gonadotropins, Equine / Q000008:administration & dosage; D006601:Hexoses / Q000032:analysis*; D037102:Lectins; D008647:Mesocricetus; D009865:Oocytes / Q000254:growth & development / Q000378:metabolism*; D015044:Zona Pellucida / Q000378:metabolism*
chemical_list: D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D006601:Hexoses; D037102:Lectins