Electrochemical detection of viable bacteria in urine and antibiotic selection.
An electrode system consisting of a basal-plane pyrolytic graphite (BPG) electrode and a porous nitrocellulose membrane filter to trap bacteria was used for the detection of bacteria in urine. The peak current of a cyclic voltammogram increased with increasing initial cell concentration of Escherichia coli in urine. Urine containing from 5 x 10(2) to 5 x 10(5) cells ml-1 was measured with this system. The susceptibility of bacteria to various antibiotics was also determined from the peak current. The minimum inhibitory concentration values obtained by the electrochemical method were in good agreement with those obtained by the conventional method.
Nakamura
N
N
Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
Shigematsu
A
A
Matsunaga
T
T
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D001437:Bacteriuria / Q000175:diagnosis*; D015169:Colony Count, Microbial; D004563:Electrochemistry; D004566:Electrodes; D004926:Escherichia coli / Q000302:isolation & purification; D005374:Filtration; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods
D000900:Anti-Bacterial Agents