title: Piperine, a plant alkaloid of the piper species, enhances the bioavailability of aflatoxin B1 in rat tissues.
abstract: Piperine is known to modify the biotransformation of drugs. The effect of piperine on the metabolic activation and distribution of [3H]-aflatoxin B1 (AFB1) in rats has been described. Piperine markedly inhibited liver microsome-catalysed [3H]AFB1 binding to calf thymus DNA in vitro, in a dose dependent manner. Rats pretreated with piperine accumulated considerable [3H]AFB1 radioactivity in plasma and in the tissues examined as compared to the controls. However, piperine had no influence on hepatic [3H]AFB1-DNA binding in vivo, which could possibly be due to the null effect of piperine on liver cytosolic glutathione (GSH) 5-transferase activity. Piperine-treated rat liver microsomes demonstrated a tendency to enhance [3H]AFB1 binding to calf thymus DNA in vivo. The effect of piperine on AFB1 metabolism thus closely resembles the mode of action of SKF 525-A on biotransformation of foreign compounds.
authors.affiliation: Biochemistry Department, Vallabhbhai Patel Chest Institute, University of Delhi, India.
authors.forename: A
authors.initials: A
authors.lastname: Allameh
authors.forename: M
authors.initials: M
authors.lastname: Saxena
authors.forename: G
authors.initials: G
authors.lastname: Biswas
authors.forename: H G
authors.initials: HG
authors.lastname: Raj
authors.forename: J
authors.initials: J
authors.lastname: Singh
authors.forename: N
authors.initials: N
authors.lastname: Srivastava
mesh_terms: D016604:Aflatoxin B1 / Q000493:pharmacokinetics*; D000470:Alkaloids; D000818:Animals; D052117:Benzodioxoles; D001682:Biological Availability; D001711:Biotransformation / Q000187:drug effects; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D010880:Piperidines / Q000494:pharmacology*; D053284:Polyunsaturated Alkamides; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution; D014316:Tritium
chemical_list: D000470:Alkaloids; D052117:Benzodioxoles; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D014316:Tritium; D016604:Aflatoxin B1; C008922:piperine