Fatty acids of healthy and periodontally diseased root substance in human teeth.
In this comparative study, samples of root substance from healthy and periodontally diseased human teeth were analyzed for fatty acids by means of a gas-chromatographic technique. The sample preparation procedure allowed specimens from individual teeth to be analyzed. The content of the fatty acids C16:0, C16:1, C18:0, and C18:1 in the superficial layer of the periodontally diseased teeth was significantly higher than that of healthy teeth. In the inner layer, there was no such difference. Four different 3-hydroxy fatty acids (3-OH C14:0, 3-OH C15:0, 3-OH C16:0, and 3-OH C17:0) were identified by combined gas-chromatography/mass-spectrometry in the tooth substance of periodontally diseased teeth. This indicates the presence of lipid A, which is the toxic component of bacterial lipopolysaccharides.
Lygre
H
H
Department of Dental Materials, School of Dentistry, Bergen, Norway.
Solheim
E
E
Gjerdet
N R
NR
Skaug
N
N
D002849:Chromatography, Gas; D003739:Dental Cementum / Q000737:chemistry; D003804:Dentin / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis*; D006801:Humans; D008050:Lipid A / Q000032:analysis*; D013058:Mass Spectrometry; D010510:Periodontal Diseases / Q000378:metabolism* / Q000382:microbiology; D014092:Tooth Root / Q000737:chemistry*
D005227:Fatty Acids; D008050:Lipid A