Induction of macrophage interleukin-1 production by Listeria monocytogenes hemolysin.
Listeriolysin O produced by a hemolytic strain of Listeria monocytogenes was purified from the ammonium sulfate precipitate of a culture supernatant through the steps of ion-exchange chromatography and gel filtration. The purified hemolysin finally gave a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a molecular weight of 58,000. When peritoneal exudate macrophages were stimulated with purified hemolysin, we found a high level of IL-1 activity as determined by thymocyte costimulator assay in the culture supernatant. Cell-associated and intracellular IL-1 activity was also detected. The activity in the supernatant or membrane was blocked by polyclonal antibody to murine IL-1 alpha. Moreover, IL-1-specific mRNA expression could be detected in the macrophages stimulated with listeriolysin O by Northern blot analysis. Possible contamination by LPS of the listeriolysin O preparation did not seem to contribute to the induction of macrophage IL-1 production.
Tsukada
H
H
Department of Bacteriology, Niigata University School of Medicine, Japan.
Kawamura
I
I
Fujimura
T
T
Igarashi
K
K
Arakawa
M
M
Mitsuyama
M
M
D000818:Animals; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D001427:Bacterial Toxins; D002478:Cells, Cultured / Q000737:chemistry / Q000187:drug effects; D003470:Culture Media / Q000032:analysis; D006360:Heat-Shock Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000302:isolation & purification; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D008089:Listeria monocytogenes / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010529:Peritoneal Cavity / Q000166:cytology; D012333:RNA, Messenger / Q000032:analysis
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003470:Culture Media; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D007375:Interleukin-1; D012333:RNA, Messenger; C038705:hlyA protein, Listeria monocytogenes