Direct DNA sequencing of PCR products.
Experiments are described which elucidate some of the technical problems associated with the direct sequencing of polymerase chain reaction (PCR) amplified DNA. Sequencing primer purity, labeling methodology, and template preparation were explored. Conditions are presented for the routine sequencing of single- and double-stranded PCR products.
Zimmerman
L J
LJ
Environmental Health Research and Testing, Inc., Research Triangle Park, NC 27709.
Fuscoe
J C
JC
D001483:Base Sequence; D002872:Chromosome Deletion; D004252:DNA Mutational Analysis; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
D007041:Hypoxanthine Phosphoribosyltransferase