title: Characterization of the secondary structure of calmodulin in complex with a calmodulin-binding domain peptide.
abstract: The interaction between calcium-saturated chicken calmodulin and a peptide corresponding to the calmodulin-binding domain of the chicken smooth muscle myosin light chain kinase has been studied by multinuclear and multidimensional nuclear magnetic resonance methods. Extensive 1H and 15N resonance assignments of calmodulin in the complex have been obtained from the analysis of two- and three-dimensional nuclear magnetic resonance spectra. The assignment of calmodulin in the complex was facilitated by the use of selective labeling of the protein with alpha-15N-labeled valine, alanine, lysine, leucine, and glycine. These provided reference points during the main-chain-directed analysis of three-dimensional spectra of complexes prepared with uniformly 15N-labeled calmodulin. The pattern of nuclear Overhauser effects (NOE) seen among main-chain amide NH, C alpha H, and C beta H hydrogens indicates that the secondary structure of the globular domains of calmodulin in the complex closely corresponds to that observed in the calcium-saturated state of the protein in the absence of bound peptide. However, the backbone conformation of residues 76-84 adopts an extended chain conformation upon binding of the peptide in contrast to its helical conformation in the absence of peptide. A sufficient number of NOEs between the globular domains of calmodulin and the bound peptide have been found to indicate that the N- and C-terminal regions of the peptide interact with the C- and N-terminal domains of calmodulin, respectively. The significance of these results are discussed in terms of recently proposed models for the structure of calmodulin-peptide complexes.
authors.affiliation: Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
authors.forename: S M
authors.initials: SM
authors.lastname: Roth
authors.forename: D M
authors.initials: DM
authors.lastname: Schneider
authors.forename: L A
authors.initials: LA
authors.lastname: Strobel
authors.forename: M F
authors.initials: MF
authors.lastname: Van Berkum
authors.forename: A R
authors.initials: AR
authors.lastname: Means
authors.forename: A J
authors.initials: AJ
authors.lastname: Wand
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002147:Calmodulin / Q000737:chemistry*; D002148:Calmodulin-Binding Proteins / Q000737:chemistry*; D002645:Chickens; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000737:chemistry / Q000201:enzymology; D009219:Myosin-Light-Chain Kinase / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation; D013329:Structure-Activity Relationship
chemical_list: D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D010455:Peptides; D009219:Myosin-Light-Chain Kinase