title: Effects of exogenous putrescine on murine preimplantation development in vitro.
abstract: After first demonstrating that murine embryos take up putrescine from the medium in which they are cultured in vitro, fertilized eggs were placed in culture and maintained for 4 days in medium supplemented with varying amounts of putrescine. Their development was monitored each day. While embryos that were cultured in putrescine-supplemented medium developed at the same rate as control embryos, a significantly higher percentage of the putrescine-treated embryos attained the blastocyst stage as compared to the control group.
authors.affiliation: Lankenau Medical Research Center, Wynnewood, Pennsylvania 19096.
authors.forename: J A
authors.initials: JA
authors.lastname: Sawicki
authors.forename: A
authors.initials: A
authors.lastname: Impellizeri
authors.forename: T G
authors.initials: TG
authors.lastname: O'Brien
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D002970:Cleavage Stage, Ovum / Q000166:cytology / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004624:Embryo Transfer; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D011247:Pregnancy; D011700:Putrescine / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D011700:Putrescine