title: Postoperative biosynthetic human growth hormone increases the strength and collagen deposition of experimental colonic anastomoses.
abstract: This study examined the effects of preoperative treatment with 2.0 mg biosynthetic human growth hormone (b-hGH)/kg/day on the bursting strength and collagen deposition of experimental left colonic anastomoses, and of intact colon from sham-operated rats. The anastomotic bursting pressure was 55% higher in the b-hGH treated animals on day 2 (p less than 0.05) and 79% higher on day 4 (NS; p = 0.056), and the bursting wall tension was 65% higher on day 2 (p less than 0.05) and 112% higher on day 4 postoperatively (p less than 0.05), than saline-injected controls. The hydroxyproline content of the anastomotic segment in the b-hGH treated rats increased by 56% on day 4 (p less than 0.005) and by 30% on day 6 postoperatively (p less than 0.05), compared with controls. At 3 cm proximal to the anastomoses the defatted dry weight and hydroxyproline content of the healing colons were increased after 6 days compared with the sham-operated rats. There was, however, no difference between the b-hGH treated rats and the paired controls, indicating that growth hormone is not involved in this process.
authors.affiliation: Department of Connective Tissue Biology, University of Aarhus, Denmark.
authors.forename: H
authors.initials: H
authors.lastname: Christensen
authors.forename: H
authors.initials: H
authors.lastname: Oxlund
authors.forename: S
authors.initials: S
authors.lastname: Laurberg
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D001835:Body Weight / Q000502:physiology; D003094:Collagen / Q000187:drug effects / Q000378:metabolism*; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000601:surgery*; D005260:Female; D013006:Growth Hormone / Q000627:therapeutic use*; D006728:Hormones / Q000627:therapeutic use*; D011182:Postoperative Care; D011183:Postoperative Complications / Q000517:prevention & control*; D051381:Rats; D011919:Rats, Inbred Strains; D014945:Wound Healing / Q000502:physiology
chemical_list: D006728:Hormones; D013006:Growth Hormone; D003094:Collagen