A NeuGc-containing trisialoganglioside of bovine brain.
A N-glycolyneuraminic acid containing trisialoganglioside was isolated from bovine brains ganglioside mixture using Q-Sepharose. Its chemical structure was characterized as IV3NeuAc, II3NeuAc-NeuGc, Gg4Cer by gas-liquid chromatography, a permethylation study, sialidase degradation, TLC/enzyme-immunostaining, fast atom bombardment-mass spectrometry, fluorometric HPLC and proton nuclear magnetic resonance spectroscopy. This was unique in the mixed sialic acid constituents. (formula; see text) This accounted for 0.78% of the gangliosides. The ceramide structure was almost identical with those of major bovine brain ganglioside, as mainly composed of 18:0 fatty acid (90.9%) and d20:0 sphingosine base.
Nakao
T
T
Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science, Japan.
Kon
K
K
Ando
S
S
Hirabayashi
Y
Y
D000818:Animals; D001923:Brain Chemistry; D002240:Carbohydrate Sequence; D002417:Cattle / Q000502:physiology*; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009438:Neuraminic Acids / Q000032:analysis*; D009439:Neuraminidase / Q000378:metabolism; D012794:Sialic Acids / Q000032:analysis*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D005732:Gangliosides; D009438:Neuraminic Acids; D012794:Sialic Acids; C032592:N-glycolylneuraminic acid; D009439:Neuraminidase