Human mononuclear cell chemiluminescence and interleukin-1 release induced by Staphylococcus aureus protein A.
Staphylococcus aureus protein A (SpA) is shown to induce a temporary chemiluminescence (CL)-response of human peripheral blood mononuclear cells. Maximal activity of SpA is observed at 3 micrograms/ml and EC50 is about 1 microgram/ml. The CL response depends on extracellular calcium, and moreover is inhibited by pretreating cells with rabbit-anti-human IgG's. From glass-adhered monocytes, SpA alone (3 micrograms/ml) did not consistently affect the generation of immunoreactive interleukin-1 (IL-1). However, SpA and endotoxin (1 micrograms/ml) showed a marked synergistic effect on IL-1 release. The results suggest that SpA interacts with membrane-bound Ig molecules endowed with receptor functions. The CL response may be envisaged as a result of early transmembrane signalling events presumably taking place in monocytes. Apparently, hereby, the cells become primed for endotoxin-triggered IL-1 release.
Olsen
U B
UB
Department of Pharmacology, Novo Nordisk Ph-R&D, Bagsvaerd, Denmark.
D004731:Endotoxins / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology*; D008163:Luminescent Measurements; D013205:Staphylococcal Protein A / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D004731:Endotoxins; D007375:Interleukin-1; D013205:Staphylococcal Protein A; D013755:Tetradecanoylphorbol Acetate