Antitumour and immunological activity of a beta 1----3/1----6 glucan from Glomerella cingulata.
The in vivo antitumour activity of a beta 1----3/1----6 glucan from the fungus Glomerella cingulata was investigated in vivo. The glucan exhibited a strong inhibition of tumour growth of the allogeneic Sarcoma-180 as well as the syngeneic DBA/2-MC.SC-1 fibrosarcoma with inhibition ratios up to 100%. Against the hormone sensitive Noble-Nb-R prostate carcinoma the glucan alone showed a moderate antitumour effect, whereas in combination with diethylstilbestrol an almost complete regression of the tumour could be achieved. It could be demonstrated that a highly ordered structure of the glucan is not essential for the antitumour activity. Since the glucan expressed no direct cytotoxic effects, the immunomodulating activity was investigated in vitro in order to get an indication for a possible mode of action. In the lymphocyte transformation assay the glucan at a dose of 100 micrograms/ml caused a fourfold increase in the proliferation of murine spleen lymphocytes. Moreover, the glucan stimulated the phagocytosis of zymosan by bone marrow macrophages up to 100%. However, the glucan was not able to render macrophages cytotoxic against P-815 mastocytoma cells.
Gomaa
K
K
Institut für Pharmazie, Universität Regensburg, Federal Republic of Germany.
Kraus
J
J
Rosskopf
F
F
Röper
H
H
Franz
G
G
D000818:Animals; D004054:Diethylstilbestrol / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D005354:Fibrosarcoma / Q000139:chemically induced / Q000188:drug therapy*; D005936:Glucans / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008262:Macrophage Activation / Q000187:drug effects; D008297:Male; D012515:Mast-Cell Sarcoma / Q000188:drug therapy* / Q000276:immunology; D008748:Methylcholanthrene; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D011471:Prostatic Neoplasms / Q000188:drug therapy*; D051381:Rats; D012510:Sarcoma 180 / Q000188:drug therapy*; D012566:Sizofiran / Q000494:pharmacology
D005936:Glucans; C033671:epiglucan; D008748:Methylcholanthrene; D004054:Diethylstilbestrol; D012566:Sizofiran