Effects of dietary fatty acids on the early stages of neoplastic induction in the rat pancreas. Changes in fatty acid composition and development of atypical acinar cell nodules.
Diets enriched with fat, especially unsaturated fat, promote experimental pancreatic carcinogenesis, but little is known of the effects of individual fatty acids. The effect of stearic and oleic acid on pancreatic fatty acids and atypical acinar cell nodules (preneoplastic lesions) was studied in 14-day-old weanling male Leeds strain rats (n = 60) given the carcinogen azaserine. Rats were allocated to one of six groups: untreated controls (n = 10), 20% stearic acid diet (n = 10), 20% oleic acid diet (n = 10), carcinogen alone (n = 10), carcinogen plus 20% stearic acid diet (n = 10) or carcinogen plus 20% oleic acid diet (n = 10). Azaserine was administered by intraperitoneal injection in a dose of 30 mg/kg at 2, 3 and 4 weeks of age. When total lipid extracts of pancreas were examined, there was an increase in stearic acid in the stearic acid fed group and an increase in oleic acid in the oleic acid fed group, irrespective of carcinogen treatment. The relative content of all other pancreatic fatty acids was suppressed by feeding oleic acid. At 26 weeks, the number and volumetric indices of pancreatic atypical acinar cell nodules was increased only in rats given azaserine and oleic acid. The enhancing effect of oleic acid on pancreatic carcinogenesis may be associated with pancreatic fatty acid changes.
Khoo
D E
DE
Department of Surgery, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Flaks
B
B
Oztas
H
H
Williamson
R C
RC
Habib
N A
NA
D000818:Animals; D001377:Azaserine; D004041:Dietary Fats / Q000633:toxicity*; D004357:Drug Synergism; D005227:Fatty Acids / Q000032:analysis / Q000633:toxicity*; D008297:Male; D009829:Oleic Acids / Q000633:toxicity; D010179:Pancreas / Q000737:chemistry; D010190:Pancreatic Neoplasms / Q000139:chemically induced* / Q000473:pathology; D011230:Precancerous Conditions / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D013229:Stearic Acids / Q000633:toxicity
D004041:Dietary Fats; D005227:Fatty Acids; D009829:Oleic Acids; D013229:Stearic Acids; D001377:Azaserine