title: A monoclonal antibody which recognizes the inner and outer segments of the photoreceptor cells in the vertebrate retina.
abstract: A monoclonal antibody (MAb-1E7), generated against bovine retinal homogenate, labeled the outer and inner segment layers of the vertebrate retina. Immuno-electron microscopic observation clearly demonstrated that antigen(s) bound by MAb-1E7 was localized in the cell membrane of the outer segment and the distal portion of the inner segment. Western blot analysis revealed that MAb-1E7 recognized 40 kD- and 27 kD-polypeptides. Mouse retina with hereditary photoreceptor degeneration (C3H/He and CBA strains) did not involve the MAb-1E7 immunoreactive structures. The present immunocytochemical observation demonstrated that MAb-1E7 was highly specific to the outer segment of the photoreceptor cells and, therefore, can be a useful marker for the cells.
authors.affiliation: Third Department of Anatomy, Okayama University Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ono
authors.forename: A
authors.initials: A
authors.lastname: Tokunaga
authors.forename: N
authors.initials: N
authors.lastname: Shibata
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Kurose
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D051379:Mice; D008815:Mice, Inbred Strains; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D010786:Photoreceptor Cells / Q000276:immunology*; D011784:Quail; D051381:Rats; D012160:Retina / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal