title: Functional innervation of spinal cord tissue by fetal neocortical grafts in oculo: an electrophysiological study.
abstract: The ability of fetal neocortex transplants, to functionally innervate maturated cervical spinal cord grafts in oculo, was investigated in rats. We found that a neocortex co-graft will grow and develop in contact with a spinal cord graft, and will generate a functional input to maturated spinal cord tissue which can be activated by electrical stimulation of the neocortex graft. Our data suggest that orthodromic stimulation of this pathway causes short latency, transient excitations of spinal graft neurons. These appear to be mediated by an excitatory amino acid receptor since the response was noncompetitively antagonized by kynurenic acid. Kynurenic acid also noncompetitively antagonized the excitatory effects of glutamate superfused over single spinal cord grafts. The mechanism of the excitation probably does not involve an NMDA (N-methyl-D-aspartate) receptor since APV (2-amino-5-phosphonovalerate) did not alter the spinal graft neuronal responses to neocortical co-graft stimulation. These data suggest that fetal neocortex can functionally innervate maturated cervical spinal cord in the in oculo graft preparation. The in oculo spinal cord graft model may thus provide a unique test system for studies of the influence of drugs and other manipulations that might alter cortico-spinal pathway development as well as influence reestablishment of neuronal pathways after spinal cord injury.
authors.affiliation: Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80262.
authors.forename: M R
authors.initials: MR
authors.lastname: Palmer
authors.forename: A
authors.initials: A
authors.lastname: Henschen
authors.forename: K
authors.initials: K
authors.lastname: Trok
authors.forename: J L
authors.initials: JL
authors.lastname: Hudson
authors.forename: B J
authors.initials: BJ
authors.lastname: Hoffer
authors.forename: L
authors.initials: L
authors.lastname: Olson
mesh_terms: D000200:Action Potentials; D000818:Animals; D016380:Brain Tissue Transplantation / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D004558:Electric Stimulation; D005123:Eye; D016332:Fetal Tissue Transplantation / Q000502:physiology; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007150:Immunohistochemistry; D007736:Kynurenic Acid / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D013116:Spinal Cord / Q000502:physiology*; D016042:Transplantation, Heterotopic
chemical_list: D005904:Glial Fibrillary Acidic Protein; D007736:Kynurenic Acid