title: Energy depletion and autophagy. Cytochemical and biochemical studies in isolated rat hepatocytes.
abstract: In this paper, data dealing with the sensitivity of autophagy towards partial ATP depletion in isolated rat hepatocytes are reviewed. Partial reduction of intracellular ATP causes: (1) a decrease of proteolytic flux; (2) a decrease in uptake of cytosolic components into the autophagic-lysosomal compartment; (3) either a decrease or no change in the ratio between volume densities of autophagosomes and lysosomes, depending on whether or not the cytosolic phosphate potential is affected; and (4) impairment of the lysosomal proton pump. It is concluded that the consecutive steps of autophagy all respond to relatively small changes of intracellular ATP concentration.
authors.affiliation: Laboratory of Cell Biology and Histology, Academic Medical Centre, Amsterdam, The Netherlands.
authors.forename: J P
authors.initials: JP
authors.lastname: Schellens
authors.forename: A J
authors.initials: AJ
authors.lastname: Meijer
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004734:Energy Metabolism; D006651:Histocytochemistry; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure*; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000255:Adenosine Triphosphate