Progesterone and oestradiol increase cytosolic Ca2+ in single rat hepatocytes.
Progesterone (300-400 microM) and oestradiol (25-200 microM) induce a prompt rise in the cytosolic free Ca2+ concentration (free Ca) in single rat hepatocytes, but testosterone, cortisol and dexamethasone do not. These increases are dependent on the presence of extracellular Ca2+. Both progesterone and oestradiol block phenylephrine-induced free Ca oscillations. These data suggest a certain specificity of the response of free Ca to steroids and may explain some of the non-genomic effects of these steroids on hepatocytes.
Sanchez-Bueno
A
A
Department of Human Anatomy and Cell Biology, University of Liverpool, U.K.
Sancho
M J
MJ
Cobbold
P H
PH
D000818:Animals; D002478:Cells, Cultured; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004533:Egtazic Acid / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010656:Phenylephrine / Q000494:pharmacology; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D010656:Phenylephrine; D011374:Progesterone; D004958:Estradiol; D004533:Egtazic Acid