Nebulized vs. instilled exogenous surfactant in an adult lung injury model.
Three days after subcutaneous injection of N-nitroso-N-methylurethane (NNNMU) to induce lung injury, adult rabbits were mechanically ventilated and lung function was evaluated. Each animal then received either nebulized Survanta (Neb Surv), nebulized saline (Neb Saline), nebulized gas alone (Neb Gas), or tracheally instilled Survanta (Inst Surv). The ventilation efficiency index (VEI) value increased significantly compared with pretreatment values (P less than 0.01) over a 3-h treatment period for the Neb Surv animals, whereas VEI values for the other three groups decreased after treatment (P less than 0.05). Arterial PO2-to-fraction of inspired O2 ratios and dynamic compliance values significantly decreased after treatment for the Inst Surv group (P less than 0.05). Pressure-volume curves demonstrated a significantly greater volume at maximal pressure for the Neb Surv group compared with each of the other groups studied (P less than 0.01). The calculated quantity of surfactant recovered in lung tissue for the Neb Surv group was only 4.9 +/- 1.0 mg lipid/kg compared with 100 mg lipid/kg delivered to the Inst Surv group. Surfactant administered as an aerosol resulted in modest physiological improvements in this model of lung injury and was superior to the tracheal instillation technique.
Lewis
J
J
Department of Pediatrics, Harbor-UCLA Medical Center, Torrance 90509.
Ikegami
M
M
Higuchi
R
R
Jobe
A
A
Absolom
D
D
D000336:Aerosols; D000818:Animals; D001784:Blood Gas Analysis; D006863:Hydrogen-Ion Concentration; D007442:Intubation, Intratracheal; D008168:Lung / Q000378:metabolism; D008170:Lung Compliance / Q000187:drug effects; D008171:Lung Diseases / Q000139:chemically induced* / Q000503:physiopathology; D009330:Nebulizers and Vaporizers; D009605:Nitrosomethylurethane; D011663:Pulmonary Surfactants / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology*; D011817:Rabbits; D012129:Respiratory Function Tests
D000336:Aerosols; D011663:Pulmonary Surfactants; D009605:Nitrosomethylurethane