Low cost device for electrotransformation and its application to the highly efficient transformation of Escherichia coli and Enterococcus faecalis.
A simple, low cost device for electrotransformation has been designed and constructed. The cost of the circuit was only $150. Maximum field strength of 12,000 V/cm with an actual time constant up to 11 msec was obtained with a newly designed circuitry and a 0.1-cm electrode gap cuvette. Eschericia coli strains DH1, DH5 alpha, and LE392 were transformed at an efficiency of 10(9)/micrograms DNA with plasmids pUC18 and pBR322. E. faecalis strain OG1X was transformed at an efficiency of 0.9 x 10(5)/micrograms DNA without treatment with glycine.
Fujimoto
S
S
Department of Microbiology, School of Medicine, Gunma University, Maebashi, Japan.
Hashimoto
H
H
Ike
Y
Y
D004269:DNA, Bacterial / Q000235:genetics; D004558:Electric Stimulation; D013293:Enterococcus faecalis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D014169:Transformation, Bacterial
D004269:DNA, Bacterial