Sulfated glycoprotein-2 (SGP-2) mRNA is expressed in rat striatal astrocytes following ibotenic acid lesions.
A combination of in situ hybridization and immunocytochemistry (ICC) was used to identify the cells that contained mRNA for sulfated glycoprotein-2 (SGP-2) in adult male rats after striatal ibotenic acid (IA) lesioning. Astrocytic responses were monitored by ICC for glial fibrillary acidic protein (GFAP). IA lesioning that caused death of striatal neurons also stimulated astrocytic responses as monitored by GFAP and SGP-2. The SGP-2 immunoreactivity showed punctate deposits in the lesioned striatum without any apparent cellular localization. By in situ hybridization combined with ICC, SGP-2 mRNA was localized in astrocytes that were GFAP-immunopositive. These data suggest that reactive astrocytes may express SGP-2 which may be eventually secreted.
Pasinetti
G M
GM
Neurogerontology Division, Andrus Gerontology Center, University of Southern California, Los Angeles 90089-0191.
Finch
C E
CE
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D016923:Cell Death / Q000187:drug effects; D051152:Clusterin; D003342:Corpus Striatum / Q000166:cytology / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis / Q000276:immunology; D006023:Glycoproteins / Q000096:biosynthesis*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D007051:Ibotenic Acid / Q000633:toxicity*; D007150:Immunohistochemistry; D008297:Male; D018832:Molecular Chaperones; D009693:Nucleic Acid Hybridization; D016612:Paraffin Embedding; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011916:Rats, Inbred F344
D051152:Clusterin; D005904:Glial Fibrillary Acidic Protein; D006023:Glycoproteins; D018832:Molecular Chaperones; D012333:RNA, Messenger; D007051:Ibotenic Acid