Transient heat shock in mid-G1-phase of the C6 glioma cell cycle impairs entry into S-phase.
Synchronous populations of rat C6 glioma cells have been obtained by a mitotic selection procedure and used to establish the effect of transient temperature elevation on their progression through the cell cycle. A transient 3-h exposure to a 42 degree C heat shock within defined area in the mid-G1-phase of the cell cycle has been demonstrated to impair progression to S-phase. This anti-proliferative heat shock effect was not apparent in asynchronous cell populations. A small, but significant, decrease in 3H-thymidine incorporation was observed (approximately 5%) following an 8-h exposure to heat shock.
Martin
M L
ML
Department of Pharmacology, University College, Dublin, Ireland.
Regan
C M
CM
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D016193:G1 Phase / Q000502:physiology*; D005910:Glioma / Q000378:metabolism*; D006358:Hot Temperature / Q000009:adverse effects*; D051381:Rats; D016196:S Phase / Q000502:physiology*; D013936:Thymidine / Q000378:metabolism
D004247:DNA; D013936:Thymidine