title: Fractional incorporation of radionucleotides, a marker of in vitro tumor cell chemosensitivity in colorectal cancer.
abstract: Working with the antimetabolic chemopredictivity assay on short term cultures we evidenced a correlation between in vitro chemosensitivity of colorectal cancer and fractional incorporation of radionucleotides. Four different drugs (5-FU, mitomycin C, 4'-iododeoxydoxorubicin and ruboxyl, a nitroxyl derivative of daunorubicin) were tested on 102 tumor cultures. The extent of 3H-TdR and 3H-UdR incorporation into DNA and RNA in the related control cultures was in relationship with the chemosensitivity of the tumor. Along with the labeling index this simple metabolic-kinetic trait may gain a role for the screening of tumor phenotypes sensitive to chemotherapy.
authors.affiliation: Institute of 3rd Clinical Medicine, University La Sapienza, Rome, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Franchi
authors.forename: P
authors.initials: P
authors.lastname: Seminara
authors.forename: D
authors.initials: D
authors.lastname: Giannarelli
authors.forename: N
authors.initials: N
authors.lastname: Konovalova
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D004273:DNA, Neoplasm / Q000096:biosynthesis; D003630:Daunorubicin / Q000031:analogs & derivatives / Q000494:pharmacology; D004317:Doxorubicin / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008113:Liver Neoplasms / Q000556:secondary; D016685:Mitomycin / Q000494:pharmacology; D012334:RNA, Neoplasm / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D014529:Uridine / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D014316:Tritium; D016685:Mitomycin; C053044:4'-deoxy-4'-iododoxorubicin; D004317:Doxorubicin; C042480:ruboxyl; D013936:Thymidine; D014529:Uridine; D003630:Daunorubicin