Immunity to Haemonchus contortus and the cellular response to helminth antigens in the mammary gland of non-lactating sheep.
Cellular exudates induced by infusion with helminth antigens were examined in non-lactating mammary glands of ewes immune to infection with the abomasal nematode, Haemonchus contortus. Secondary immunological responsiveness was expressed in two ways. Firstly, antigens from adult H. contortus elicited larger eosinophil-rich cellular exudates in immune compared to non-immune ewes. In this situation, secondary responsiveness in the mammary gland must have been generated through abomasal infection with the parasite. Secondly, repeated infusion with the antigens from adult H. contortus increased the size of cellular exudates in both immune and non-immune ewes. Eosinophils predominated but numbers of macrophages and lymphocytes were also increased. In this second situation, secondary responsiveness must have been either supplemented in immune ewes or derived completely in non-immune ewes by contact with helminth antigens through the mammary gland. The helminth antigens which induce eosinophil exudates in the mammary gland may not be potently protective against H. contortus. Furthermore, eosinophil exudation may not be an in vivo correlate of immunity which is directly useful for discriminating protective antigens and applicable to vaccine development. Infusion with antigens from adult forms of either H. contortus or Trichostrongylus colubriformis elicited cellular exudates equally well in immune ewes primed by infusion with H. contortus adult antigens 7 days beforehand. In addition, antigens from infective larvae of H. contortus elicited cellular exudates more potently than antigens from adult worms. However, vaccination with irradiated larvae has shown that species-specific protective immunity for H. contortus is stronger than cross-protective immunity conferred by T. colubriformis.(ABSTRACT TRUNCATED AT 250 WORDS)
Adams
D B
DB
CSIRO Division of Animal Health, Pastoral Research Laboratory, Armidale, New South Wales, Australia.
Colditz
I G
IG
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D005260:Female; D006188:Haemonchiasis / Q000276:immunology*; D006189:Haemonchus / Q000276:immunology*; D007111:Immunity, Cellular; D007962:Leukocytes / Q000276:immunology*; D008321:Mammary Glands, Animal / Q000276:immunology*; D012756:Sheep; D014254:Trichostrongylus / Q000276:immunology
D000947:Antigens, Helminth