Mutagenicity in V79 cells of N-nitrosobis(2-hydroxypropyl)amine and N-nitrosobis(2-oxopropyl)amine activated by tissues from hamsters fed low and high fat diets.
Hepatocytes and pancreas duct tissues from male Syrian hamsters fed high-fat (HFD) and low-fat (LFD) diets were used to activate N-nitrosobis(2-hydroxypropyl)amine (BHP) and N-nitrosobis(2-oxopropyl)amine (BOP) in the V79 cell mutagenicity assay. V79 DNA alkylation by BHP and BOP was also measured. There was a 3.5-fold increase in BHP mutagenicity but only a 1.4-fold increase in BOP mutagenicity when hepatocytes from HFD-fed hamsters were used over the mutagenicity when hepatocytes from LFD-fed hamsters were used. When pancreas duct tissue was the activating system there was a 2-fold increase in BHP and BOP mutagenicity. O6-Methylguanine levels in V79 DNA rose 4-fold when hepatocytes from HFD-fed hamsters were used to activate BOP but they declined when BHP was the alkylating agent.
Lawson
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Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68198-6805.
D000478:Alkylation; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage*; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D016296:Mutagenesis / Q000187:drug effects*; D009152:Mutagenicity Tests; D009602:Nitrosamines / Q000633:toxicity*; D010183:Pancreatic Ducts / Q000187:drug effects / Q000378:metabolism*
D002273:Carcinogens; D004041:Dietary Fats; D009602:Nitrosamines; C012457:diisopropanolnitrosamine; D006147:Guanine; C013622:nitrosobis(2-oxopropyl)amine; D004247:DNA; C008449:O-(6)-methylguanine