Assignment of the 1H NMR spectrum and secondary structure elucidation of the single-stranded DNA binding protein encoded by the filamentous bacteriophage IKe.
By means of 2D NMR techniques, all backbone resonances in the 1H NMR spectrum of the single-stranded DNA binding protein encoded by gene V of the filamentous phage IKe have been assigned sequence specifically (at pH 4.6, T = 298 K). In addition, a major part of the side chain resonances could be assigned as well. Analysis of NOESY data permitted the elucidation of the secondary structure of IKe gene V protein. The major part of this secondary structure is present as an antiparallel beta-sheet, i.e., as two beta-loops which partly combine into a triple-stranded beta-sheet structure, one beta-loop and one triple-stranded beta-sheet structure. It is shown that a high degree of homology exists with the secondary structure of the single-stranded DNA binding protein encoded by gene V of the distantly related filamentous phage M13.
van Duynhoven
J P
JP
Nijmegen SON Research Centre for Molecular Design, Structure and Synthesis, Faculty of Science, University of Nijmegen, The Netherlands.
Folkers
P J
PJ
Prinse
C W
CW
Harmsen
B J
BJ
Konings
R N
RN
Hilbers
C W
CW
D000595:Amino Acid Sequence; D003090:Coliphages / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012689:Sequence Homology, Nucleic Acid; D012996:Solutions; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D012996:Solutions; D014764:Viral Proteins; C062871:gene 5 protein, Enterobacteria phage Ike