Human neutrophil granule cationic protein CAP37 is a specific macrophage chemotaxin that shares homology with inflammatory proteinases.
Cationic antimicrobial protein CAP37 (Mr = 37 kD) is derived from the azurophilic granules of human PMN. In vitro and in vivo studies demonstrate that CAP37 is a novel monocyte-specific chemoattractant. The N-terminal amino acid sequence of CAP37 shares significant homology with a number of inflammatory molecules with protease activity including elastase and cathepsin G. However, substitutions in the catalytic triad (serine for a histidine at position 41 and glycine for a serine at position 175), may account for its lack of serine protease activity. A full length cDNA for CAP37 was identified in an HL60 cDNA library screened with oligonucleotide probes designed from the N-terminal amino acid sequence. Sequencing of the cDNA reveals a protein of 225 amino acids with significant nucleotide homology to cathepsin G and human neutrophil elastase.
Morgan
J G
JG
Genelabs Inc., Redwood City, CA 94063.
Pereira
H A
HA
Sukiennicki
T
T
Spitznagel
J K
JK
Larrick
J W
JW
D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D002352:Carrier Proteins; D002630:Chemotactic Factors; D004247:DNA / Q000235:genetics; D006801:Humans; D007249:Inflammation / Q000201:enzymology*; D008264:Macrophages; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases / Q000737:chemistry*
C062991:AZU1 protein, human; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002352:Carrier Proteins; D002630:Chemotactic Factors; D004247:DNA; D012697:Serine Endopeptidases