Cloning, sequencing and characterization of the Saccharomyces cerevisiae URA7 gene encoding CTP synthetase.
The URA7 gene of Saccharomyces cerevisiae encodes CTP synthetase (EC 6.3.4.2) which catalyses the conversion of uridine 5'-triphosphate to cytidine 5'-triphosphate, the last step of the pyrimidine biosynthetic pathway. We have cloned and sequenced the URA7 gene. The coding region is 1710 bp long and the deduced protein sequence shows a strong degree of homology with bacterial and human CTP synthetases. Gene disruption shows that URA7 is not an essential gene: the level of the intracellular CTP pool is roughly the same in the deleted and the wild-type strains, suggesting that an alternative pathway for CTP synthesis exists in yeast. This could involve either a divergent duplicated gene or a different route beginning with the amination of uridine mono- or diphosphate.
Ozier-Kalogeropoulos
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Centre de Génétique Moléculaire du C.N.R.S. Université Pierre et Marie Curie, Gif-sur-Yvette, France.
Fasiolo
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Adeline
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MT
Collin
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Lacroute
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D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D019731:Carbon-Nitrogen Ligases; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics*; D008025:Ligases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D016415:Sequence Alignment
D005656:Fungal Proteins; D008025:Ligases; D019731:Carbon-Nitrogen Ligases; C019951:CTP synthetase