title: Ssn6-Tup1 is a general repressor of transcription in yeast.
abstract: The homeodomain protein alpha 2 and the SRF-like protein Mcm1 are required to establish cell type in the yeast Saccharomyces cerevisiae. Together, these regulatory proteins recognize a specific DNA operator, marking a set of genes for transcriptional repression. In this paper, we show that occupancy of the operator by alpha 2-Mcm1 is not sufficient to bring about repression. Rather, repression is effected only when Ssn6 (a TPR protein) and Tup1 (a beta-transducin repeat protein) are also present in the cell. We show that Ssn6 represses transcription when brought to a promoter by a bacterial DNA-binding domain and that Tup1 is required for this repression. Based on these and other results, we propose that Ssn6-Tup1 is a general repressor of transcription in yeast, recruited to target promoters by a variety of sequence-specific DNA-binding proteins.
authors.affiliation: Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
authors.forename: C A
authors.initials: CA
authors.lastname: Keleher
authors.forename: M J
authors.initials: MJ
authors.lastname: Redd
authors.forename: J
authors.initials: J
authors.lastname: Schultz
authors.forename: M
authors.initials: M
authors.lastname: Carlson
authors.forename: A D
authors.initials: AD
authors.lastname: Johnson
mesh_terms: D015151:Immunoblotting; D008957:Models, Genetic; D010957:Plasmids; D012097:Repressor Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012097:Repressor Proteins