Intermediates and products of synthetic lignin (dehydrogenative polymerizate) degradation by Phlebia tremellosa.
Agitated, nitrogen-limited cultures of Phlebia tremellosa caused substantial changes in the distribution of 14C-labelled synthetic lignin (dehydrogenative polymerizate [DHP]) between water-soluble, dioxane-soluble, alkali-soluble, and insoluble fractions before much lignin carbon was metabolized to CO2. First, the insoluble form increased at the expense of the dioxane-soluble form. Later, the amounts of alkali-soluble and water-soluble 14C increased, and release of 14CO2 began. The molecular weight distribution of the dioxane-soluble lignin remained constant during degradation, but that of the water-soluble fraction changed to higher molecular weights. Culture agitation accelerated the attachment of suspended DHP to the mycelia and stimulated production of water-soluble 14C and 14CO2. The nonionic detergent Tween 80 also hastened release of 14CO2 and increased the early conversion of dioxane-soluble DHP to the alkali-soluble and insoluble forms. Oxidative polymerization is suggested as the first step in degradation of DHP by P. tremellosa.
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National Research Council of Canada, Biotechnology Research Institute, Montreal, Quebec.
D001673:Biodegradation, Environmental; D003470:Culture Media; D005658:Fungi / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism*; D011136:Polysorbates / Q000494:pharmacology; D012995:Solubility; D014732:Vibration
D003470:Culture Media; D011136:Polysorbates; D008031:Lignin