Membrane pore size and filtration selectivity in cryoglobulinemic plasma fractionation.
Efficiency of albumin (Alb) and immunoglobulin G (IgG) separation from cryoprecipitable proteins (CPP) and IgM was compared between a membrane plasma fractionator with a mean pore diameter (MPD) of 0.1 micron, surface area of 0.65 m2, and a new large pore diameter filter with a MPD of 4.3 microns, and surface area of 0.14 m2. In five in vitro tests at 4 degrees C using polyclonal cryoglobulinemic (CG) plasma from a patient treated by plasma exchange (PE), the large pore filter retained only 22% of the mass of Alb, and a comparable amount of CPP and IgM, as compared to the plasma fractionator, at a fraction of its total retention capacity. The new filter can replace PE in treatment of polyclonal CG.
Zborowski
M
M
Department of Biomedical Engineering and Applied Therapeutics, Cleveland Clinic Foundation, Ohio 44195-5069.
Malchesky
P S
PS
Duggan
P A
PA
Koo
A P
AP
D003449:Cryoglobulinemia / Q000097:blood / Q000628:therapy*; D003450:Cryoglobulins / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D008567:Membranes, Artificial; D010951:Plasma Exchange / Q000295:instrumentation*; D010956:Plasmapheresis / Q000295:instrumentation*; D012709:Serum Albumin / Q000378:metabolism
D003450:Cryoglobulins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008567:Membranes, Artificial; D012709:Serum Albumin; D005340:Fibrinogen