title: Nonreplicating viral vectors as potential vaccines: recombinant canarypox virus expressing measles virus fusion (F) and hemagglutinin (HA) glycoproteins.
abstract: The development of canarypox virus (CPV) recombinants expressing the hemagglutinin (HA) and fusion (F) glycoproteins of measles virus (MV) is described. Inoculation of the CPV-MV recombinants into avian or nonavian tissue culture substrates led to the expression of authentic MVF and MVHA as determined by radioimmunoprecipitation and surface immunofluorescence. In contrast to avian-derived tissue culture, no productive replication of the CPV recombinant was evident in tissue culture cells derived from nonavian origin. On inoculation of dogs, a species restricted for avipoxvirus replication, the recombinants elicited a protective immune response against a lethal canine distemper virus (CDV) challenge. The level of MV neutralizing antibodies and the level of protection induced against CDV challenge achieved by the host-restricted CPV vector were equivalent to that obtained by vaccinia virus vectors expressing the same MV antigens.
authors.affiliation: Virogenetics Corporation, Troy, New York 12180.
authors.forename: J
authors.initials: J
authors.lastname: Taylor
authors.forename: R
authors.initials: R
authors.lastname: Weinberg
authors.forename: J
authors.initials: J
authors.lastname: Tartaglia
authors.forename: C
authors.initials: C
authors.lastname: Richardson
authors.forename: G
authors.initials: G
authors.lastname: Alkhatib
authors.forename: D
authors.initials: D
authors.lastname: Briedis
authors.forename: M
authors.initials: M
authors.lastname: Appel
authors.forename: E
authors.initials: E
authors.lastname: Norton
authors.forename: E
authors.initials: E
authors.lastname: Paoletti
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002478:Cells, Cultured; D003673:Defective Viruses / Q000235:genetics / Q000276:immunology; D004216:Distemper / Q000517:prevention & control; D004217:Distemper Virus, Canine / Q000276:immunology; D004285:Dogs; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000276:immunology*; D008459:Measles virus / Q000235:genetics / Q000276:immunology*; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000235:genetics; D011212:Poxviridae / Q000235:genetics* / Q000276:immunology; D011233:Precipitin Tests; D014614:Vaccines, Synthetic / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D014614:Vaccines, Synthetic; D014760:Viral Fusion Proteins; D014765:Viral Vaccines