title: DNA sequence of the yeast transketolase gene.
abstract: Transketolase (EC 2.2.1.1) is the enzyme that, together with aldolase, forms a reversible link between the glycolytic and pentose phosphate pathways. We have cloned and sequenced the transketolase gene from yeast (Saccharomyces cerevisiae). This is the first transketolase gene of the pentose phosphate shunt to be sequenced from any source. The molecular mass of the proposed translated protein is 73,976 daltons, in good agreement with the observed molecular mass of about 75,000 daltons. The 5'-nontranslated region of the gene is similar to other yeast genes. There is no evidence of 5'-splice junctions or branch points in the sequence. The 3'-nontranslated region contains the polyadenylation signal (AATAAA), 80 base pairs downstream from the termination codon. A high degree of homology is found between yeast transketolase and dihydroxyacetone synthase (formaldehyde transketolase) from the yeast Hansenula polymorpha. The overall sequence identity between these two proteins is 37%, with four regions of much greater similarity. The regions from amino acid residues 98-131, 157-182, 410-433, and 474-489 have sequence identities of 74%, 66%, 83%, and 82%, respectively. One of these regions (157-182) includes a possible thiamin pyrophosphate (TPP) binding domain, and another (410-433) may contain the catalytic domain.
authors.affiliation: Biochemistry Research Laboratory, Veterans Affairs Medical Center, Martinez, California 94553.
authors.forename: T S
authors.initials: TS
authors.lastname: Fletcher
authors.forename: I L
authors.initials: IL
authors.lastname: Kwee
authors.forename: T
authors.initials: T
authors.lastname: Nakada
authors.forename: C
authors.initials: C
authors.lastname: Largman
authors.forename: B M
authors.initials: BM
authors.lastname: Martin
mesh_terms: D019880:Aldehyde-Ketone Transferases; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D004271:DNA, Fungal / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010843:Pichia / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013835:Thiamine Pyrophosphate / Q000378:metabolism; D014166:Transferases / Q000737:chemistry; D014174:Transketolase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D014166:Transferases; D019880:Aldehyde-Ketone Transferases; D014174:Transketolase; C031222:formaldehyde transketolase; D013835:Thiamine Pyrophosphate