Effect of probenecid on phagocytosis and intracellular killing of Staphylococcus aureus and Escherichia coli by human monocytes and granulocytes.
The present study concerns the effects of probenecid on the phagocytosis and intracellular killing of Staphylococcus aureus and Escherichia coli by human monocytes and granulocytes. In both monocytes and granulocytes the inhibitory effect on phagocytosis was very small. Inhibition of intracellular killing of S. aureus by monocytes and granulocytes by probenecid was concentration dependent, being half-maximal at about 2 mM probenecid, and near-maximal at about 5 mM probenecid. The intracellular killing could also be inhibited when probenecid was added when this process was already started. Probenecid also inhibited the intracellular killing of E. coli by granulocytes, but not by monocytes. In the concentration range used, probenecid had no toxic effect on phagocytes or bacteria during the 2 hr of the experiments.
Buisman
H P
HP
Department of Infectious Diseases, University Hospital, Leiden, The Netherlands.
Buys
L F
LF
Langermans
J A
JA
van den Broek
P J
PJ
van Furth
R
R
D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000276:immunology; D006098:Granulocytes / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects*; D011339:Probenecid / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000276:immunology; D013997:Time Factors
D006861:Hydrogen Peroxide; D011339:Probenecid