title: Nucleotide sequence of the Clostridium thermocellum laminarinase gene.
abstract: The sequence presented (1022 bp) shows the Clostridium thermocellum laminarinase gene (lam1) and its flanking regions. The gene lam1 comprises an open reading frame of 726 nt, encoding a 242-aa protein with predicted Mr 27661. The ORF startswith the translation initiation codon ATG. This ATG codon is preceded at a spacing of 7 bp by a potential ribosome binding site (GGAGGT). A putative signal peptide was identified (the potential cleavage site is between position 27-28 aa). The comparison of the primary protein sequence with other beta-1, 3-1, 4-glucanases showed extensive homology for Bacillus amyloliqefaciens and Bacillus subtilis glucanases (identity-46.7%; similarity-57.0%).
authors.affiliation: Institute of Molecular Genetics, USSR Acad. of Sci., Moscow.
authors.forename: V V
authors.initials: VV
authors.lastname: Zverlov
authors.forename: D A
authors.initials: DA
authors.lastname: Laptev
authors.forename: V I
authors.initials: VI
authors.lastname: Tishkov
authors.forename: G A
authors.initials: GA
authors.lastname: Velikodvorskaja
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003013:Clostridium / Q000201:enzymology* / Q000235:genetics; D003062:Codon; D004269:DNA, Bacterial / Q000737:chemistry*; D005798:Genes, Bacterial; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
chemical_list: D003062:Codon; D004269:DNA, Bacterial; D004693:Glucan Endo-1,3-beta-D-Glucosidase