title: Characterizations of and interactions between bile ductule cells and hepatocytes in early stages of rat hepatocarcinogenesis induced by ethionine.
abstract: Numerous hepatic cell lineage pathways have been proposed for the development of hepatocarcinogensis induced by chemical carcinogens in rats. The roles of bile ductule cells and hepatocytes in the development of carcinogenesis were investigated using light and electron microscopic procedures to detect differences in morphology and in the phenotypic expression of antigens that are associated with each cell type. In early stages of hepatocarcinogenesis (4-10 weeks after initiation of feeding of a choline-deficient ethionine containing diet), both bile ductulelike (BDL) cells and hepatocytes were seen in mitosis. At the light microscope level, BDL cells showed intense cytoplasmic pyronin (RNA) staining and were positive for the antigens defined by monoclonal antibody 270.38 (bile ductule cells and "oval" cell marker) and glutathione-S-transferase (Yp isoform), whereas hepatocytes were positive for the antigens defined by monoclonal antibodies 270.26 and 258.26 (liver parenchymal cell markers), catalase activity (peroxisome marker) and adenosine triphospatase activity (bile canalicular marker). The authors frequently encountered BDL cells and hepatocytes in close proximity. Ultrastructural examination showed extensive plasma membrane appositions between a subset of BDL cells and hepatocytes. Desmosome structures, tight junctions, microvilli interdigitations and ATPase-positive bile canalicularlike structures were present along the contiguous plasma membrane domains of BDL cells and hepatocytes. Many of the BDL cells attached to hepatocytes were also attached to other BDL cells that had retained a basal lamina. In many cases, BDL cells connected to both hepatocytes and other BDL cells were no longer completely surrounded by basal lamina and had acquired a dual polarity as a consequence of their sharing apical and lateral membrane domains with both BDL cells and hepatocytes. BDL cells showed increased numbers of microperoxisomes (catalase positive organelles) and numerous free ribosomes. Hepatocytes showed a prominent development of the smooth endoplasmic reticulum, a feature prominent in hepatocytes within hyperplastic nodules. Since BDL cells and hepatocytes proliferate and BDL cells and hepatocytes develop intercellular junction sites, the authors propose that both cell types in early stages of carcinogenesis have the capacity to enter the cell lineage pathway leading to the development of hepatocarcinoma. Furthermore, the finding that BDL cells and hepatocytes form multiple attachment sites at the level of the plasma membrane, suggests the possibility that at some stage convergence of separate hepatic cell pathways may occur.
authors.affiliation: Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461.
authors.forename: P M
authors.initials: PM
authors.lastname: Novikoff
authors.forename: T
authors.initials: T
authors.lastname: Ikeda
authors.forename: D C
authors.initials: DC
authors.lastname: Hixson
authors.forename: A
authors.initials: A
authors.lastname: Yam
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis; D001652:Bile Ducts / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D002277:Carcinoma / Q000139:chemically induced*; D002450:Cell Communication; D002462:Cell Membrane / Q000648:ultrastructure; D016764:Cell Polarity; D004247:DNA / Q000378:metabolism; D005001:Ethionine; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D008113:Liver Neoplasms / Q000139:chemically induced*; D008830:Microbodies / Q000648:ultrastructure; D008854:Microscopy, Electron; D051381:Rats; D012270:Ribosomes / Q000648:ultrastructure
chemical_list: D000941:Antigens; D008055:Lipids; D004247:DNA; D005001:Ethionine