Identification and characterization of the ribosomal RNA-encoding genes in Clavibacter xyli subsp. cynodontis.
Clavibacter xyli subsp. cynodontis (Cxc) is a xylem-inhabiting bacterial endophyte of Bermudagrass. This organism is classified with Gram-positive, high G + C content, coryneform-actinomycete bacteria. Southern-blot analysis showed that Cxc contains only one copy of the ribosomal RNA-encoding genes (rRNA). A clone containing the rRNA genes was isolated from a genomic library of Cxc DNA cloned in the lambda EMBL3 vector. The gene cluster was partially sequenced, revealing the gene order 5'-16S-23S-5S-3', similar to that found in other prokaryotes. Low-resolution S1 mapping suggested multiple transcription start points of the rRNA operon.
Sathyamoorthy
M
M
Crop Genetics International, Hanover, MD 21076.
Alcorn
S C
SC
Lohnas
G L
GL
Anderson
J J
JJ
Uratani
B B
BB
D000192:Actinomycetales / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009876:Operon; D006109:Poaceae / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012341:RNA, Ribosomal, 5S