title: Serum bactericidal activity for Yersinia enterocolitica in hemodialysis patients: effects of iron overload and deferoxamine.
abstract: Human serum has been shown to be bactericidal for most strains of Yersinia enterocolitica. Systemic Y enterocolitica infections have been reported in iron-overloaded hemodialysis patients treated with deferoxamine. Both iron and deferoxamine are known to enhance the growth of Y enterocolitica. We inoculated sera from 12 hemodialysis patients whose serum ferritin levels ranged from 26 to 6,855 micrograms/mL (ng/mL), as well as three controls, with Yersinia organisms. After latencies of 0 to 24 hours, inoculated sera were then plated on blood agar. Bactericidal activity was demonstrated in all sera and the degree of activity did not correlate with ferritin levels. Bactericidal activity was also demonstrated in sera from three deferoxamine treated patients. We conclude that in vitro, sera of end-stage renal failure patients, with and without iron overload, are as bactericidal as control sera for Y enterocolitica and that deferoxamine therapy does not interfere with that bactericidal activity.
authors.affiliation: Department of Medicine, Thomas Jefferson University Hospital, Philadelphia, PA 19107.
authors.forename: W J
authors.initials: WJ
authors.lastname: Gaughan
authors.forename: A
authors.initials: A
authors.lastname: Beserab
authors.forename: H D
authors.initials: HD
authors.lastname: Stein
authors.forename: R A
authors.initials: RA
authors.lastname: Sirota
authors.forename: M
authors.initials: M
authors.lastname: Yudis
mesh_terms: D000328:Adult; D000368:Aged; D001770:Blood Bactericidal Activity; D003676:Deferoxamine / Q000627:therapeutic use*; D005260:Female; D005293:Ferritins / Q000032:analysis; D006801:Humans; D007501:Iron / Q000097:blood*; D007676:Kidney Failure, Chronic / Q000097:blood / Q000276:immunology / Q000628:therapy; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis; D015008:Yersinia enterocolitica / Q000276:immunology*
chemical_list: D005293:Ferritins; D007501:Iron; D003676:Deferoxamine