[Effect of pre-treatment with pyridostigmine on the stimulation of growth hormone by clonidine and GRF].
The large availability of biosynthetic GH suggested the need to define the more accurate way to make diagnosis of GH deficit. Only one stimulation test by clonidine or insulin is not enough to define a GH deficit, and this because often it's possible to get "false negative" tests. The GH is regulated by the influence of GRF and somatostatin that respectively are under the adrenergic and cholinergic control, for this reason we studied how and in which measure a cholinergic agonist (pyridostigmine) acts on GH release during the clonidine and GRF stimulation tests. We studied the area under the curve (AUC), the peak and the mean of the value of GH after clonidine or clonidine and pyridostigmine, and after GRF or GRF and pyridostigmine: we got the following results: 191 +/- 71.33 (AUC), 5.42 +/- 1.68 (peak), 2.44 +/- 0.54 (mean) after clonidine stimulation test; 1048 +/- 442.37 (AUC), 19.5 +/- 10.15 (peak) and 7.96 +/- 3.2 (mean) after clonidine and pyridostigmine (p less than 0.01); 1499 +/- 887 (AUC), 21.1 +/- 11.8 (peak) and 11.11 +/- 6.6 (mean) after GRF test and 2370 +/- 332 (AUC), 31.4 +/- 3.49 (peak) and 18.22 +/- 3.27 (mean) after GRF and pyridostigmine. The pyridostigmine effect on the simulation by clonidine and GRF is able to potentiate the stimulation of GH and allowed a more accurate diagnosis of GH deficit.
Farello
G
G
Clinica Pediatrica, L'Aquila.
De Simone
M
M
Gentile
T
T
de Matteis
F
F
D000293:Adolescent; D002648:Child; D003000:Clonidine; D003677:Deficiency Diseases / Q000175:diagnosis; D005260:Female; D013006:Growth Hormone / Q000172:deficiency / Q000187:drug effects*; D013007:Growth Hormone-Releasing Hormone; D006801:Humans; D008297:Male; D011729:Pyridostigmine Bromide / Q000494:pharmacology*
D013006:Growth Hormone; D013007:Growth Hormone-Releasing Hormone; D011729:Pyridostigmine Bromide; D003000:Clonidine
Effetto del pre-trattamento con piridostigmina sulla stimolazione del GH da parte della clonidina e del GRF.