The polymerase chain reaction and post-mortem forensic identity testing: application of amplified D1S80 and HLA-DQ alpha loci to the identification of fire victims.
The application of DNA typing methods after amplification by the polymerase chain reaction (PCR) of DNA derived from body tissues from charred fire victims was investigated. A total of 26 different tissue specimens from ten extensively burned individuals were analyzed. The samples included femoral muscle, psoas muscle, bone marrow and blood. The post-mortem period varied from 38 to 183 h. After amplifying the DNA by PCR from the various tissues, the D1S80 locus was analyzed with a high resolution polyacrylamide gel electrophoresis technique followed by silver staining and the alleles of the HLA-DQ alpha locus were detected by using a reverse dot blot format. All samples could be typed for both loci and the genotypes were consistent in the various tissues from each individual. A parentage test was performed in two cases and Mendelian inheritance of the alleles for both loci was observed.
Sajantila
A
A
Laboratory of Molecular Genetics, National Public Health Institute, Helsinki, Finland.
Ström
M
M
Budowle
B
B
Karhunen
P J
PJ
Peltonen
L
L
D000483:Alleles; D001483:Base Sequence; D001853:Bone Marrow / Q000473:pathology; D002056:Burns / Q000473:pathology*; D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000737:chemistry; D005260:Female; D005390:Fires; D005554:Forensic Medicine / Q000379:methods*; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009132:Muscles / Q000473:pathology; D010375:Pedigree; D016133:Polymerase Chain Reaction; D011180:Postmortem Changes; D016658:Psoas Muscles / Q000473:pathology
D015342:DNA Probes; D006683:HLA-DQ Antigens; D004247:DNA