title: Improved maintenance of platelet in vivo viability during storage when using a synthetic medium with inhibitors.
abstract: We report on the storage of platelet concentrates and the effects on in vivo and in vitro platelet function tests of adding the platelet inhibitors prostaglandin E1 and theophylline to a plasma-free synthetic medium and storage in containers with reduced surface-to-volume ratio. Paired in vivo studies on platelets labeled with indium 111 after 14-day storage demonstrated higher recoveries (mean +/- SD) of 23% +/- 9% and longer survivals of 109 +/- 59 hours for the test group versus 8% +/- 10% and 19 +/- 22 hours, respectively (p less than 0.01), for the control group (synthetic medium with no inhibitors and use of standard containers). The improved viability was associated with a significantly lower glycolytic rate; better maintenance of other in vitro parameters including respiratory activity, adenosine triphosphate levels, hypotonic shock response, surface glycoprotein Ib (by flow cytometry); and improved preservation of morphologic integrity (p less than 0.05). In particular, a strong inverse correlation (r = -0.91) was observed between in vivo recoveries and the fraction of platelets negative for glycoprotein Ib. We conclude that avoidance of platelet activation by a combination of inhibitors, plasma removal, and reduced container surface improves platelet respiratory activity, adenosine triphosphate and glycoprotein Ib levels, and posttransfusion viability of platelet concentrates stored for 14 days.
authors.affiliation: American Red Cross, Mid-Atlantic Region, Norfolk, Va.
authors.forename: S
authors.initials: S
authors.lastname: Holme
authors.forename: A
authors.initials: A
authors.lastname: Bode
authors.forename: W A
authors.initials: WA
authors.lastname: Heaton
authors.forename: S
authors.initials: S
authors.lastname: Sawyer
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D000527:Alprostadil / Q000032:analysis / Q000494:pharmacology*; D001792:Blood Platelets / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D001793:Blood Preservation / Q000379:methods; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D016895:Culture Media, Serum-Free / Q000032:analysis / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D010101:Oxygen Consumption / Q000502:physiology; D010980:Platelet Membrane Glycoproteins / Q000032:analysis; D013806:Theophylline / Q000032:analysis / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D016895:Culture Media, Serum-Free; D010980:Platelet Membrane Glycoproteins; D000255:Adenosine Triphosphate; D013806:Theophylline; D000527:Alprostadil