title: Expression of tumor necrosis factor-alpha and -beta transcripts in embryonal carcinoma and trophoblast cell lines: inflammation-like state as possible regulatory mechanism for ontogenesis.
abstract: TNF-alpha and TNF-beta are both involved in inflammation which regulates homeostasis in adults. We examined the mRNA expression of TNF-alpha and TNF-beta in murine embryonal carcinoma (EC) cell lines (PCC3, PCC4, ECA2 and F9) and trophoblast cell line (PL/B6) using a combined system of reverse transcription and polymerase chain reaction. Four lines of EC cells and PL/B6 expressed mRNA of both TNFs. Moreover, mRNA expression of two types of TNF-receptor and transcription factor NF-kappa B, both of which mediate a part of the biological function of TNFs, were also detected in EC and trophoblast cells. In order to clarify whether other cytokines may form a network during embryonal development, we also examined the expression of transcripts of inflammatory cytokines produced by activated macrophages (IL-1 alpha, IL-1 beta and G-CSF) or by T lymphocytes (IL-3 and IFN-gamma) in adult. We found that no IL-1 alpha transcript was expressed in any of the cell lines examined whether differentiated or not. Contrary to this, the transcript of G-CSF was continuously expressed in all cell lines, and those of IL-1 beta, IL-3 and IFN-gamma were slightly expressed in some cell lines.
authors.affiliation: Biotechnology Research Center, Teikyo University, Kawasaki, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Kohchi
authors.forename: D
authors.initials: D
authors.lastname: Mizuno
authors.forename: G
authors.initials: G
authors.lastname: Soma
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D008070:Lipopolysaccharides; D008233:Lymphotoxin-alpha / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D013698:Templates, Genetic; D013724:Teratoma / Q000235:genetics*; D014158:Transcription, Genetic; D014327:Trophoblasts / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D008070:Lipopolysaccharides; D008233:Lymphotoxin-alpha; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha