title: Heterogeneous distribution of Weibel-Palade bodies and von Willebrand factor along the porcine vascular tree.
abstract: Vessels obtained from different levels of pig vascular tree were examined by transmission electron microscope, with the aim of determining whether or not their endothelial cells contain Weibel-Palade bodies (WPB). As these organelles are known to store the von Willebrand factor (vWF), a two-step immunogold labeling of this protein also was performed. Our results showed for the first time a heterogeneous distribution of WPB along the vascular tree of the normal pig: These structures were absent from the thoracic aorta, rare in the abdominal aorta, present in myocardial capillaries, and numerous in the inferior vena cava and pulmonary artery. Atypical WPB devoid of tubules were seen in all endothelial cells. The ultrastructural labeling of vWF demonstrated its presence only in the WPB, being absent in the subendothelium, and showed the same variation in its distribution along the vascular tree as for its storage organelle. Pigs homozygous for the von Willebrand disease were found to have only the atypical WPB, and do not express the vWF.
authors.affiliation: Faculté de Médecine Saint-Antoine, Université Pierre et Marie Curie, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Gebrane-Younès
authors.forename: L
authors.initials: L
authors.lastname: Drouet
authors.forename: J P
authors.initials: JP
authors.lastname: Caen
authors.forename: L
authors.initials: L
authors.lastname: Orcel
mesh_terms: D000375:Aging / Q000378:metabolism / Q000502:physiology; D000818:Animals; D001808:Blood Vessels / Q000378:metabolism / Q000648:ultrastructure*; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D015388:Organelles / Q000648:ultrastructure*; D012016:Reference Values; D013552:Swine; D014842:von Willebrand Diseases / Q000378:metabolism / Q000473:pathology; D014841:von Willebrand Factor / Q000378:metabolism*
chemical_list: D014841:von Willebrand Factor