[Allergic rhinitis nasal epithelial cell conditioned medium stimulates growth and differentiation of basophil/mast cell and eosinophil progenitors from atopic blood].
We examined the conditions, kinetics and cell sources for basophil/mast cell and eosinophil colony stimulating activity (CSA) production by nasal epithelial cells taken from house dust (HD) nasal allergic patients. Epithelial scrapings removed from HD nasal allergic patients were grown to confluence over 7 days as a monolayer of epithelial cells in medium supplemented with fetal calf serum (FCS) on collagen coated microwell plates. CSA in nasal epithelial cells conditioned medium (CM) was assessed with density-gradient separated, nonadherent peripheral blood mononuclear cells in 14-day and 21-day methylcellulose assays. In the 14-day methylcellulose assays, the number of Eo-type colonies in the presence of either 10% or 5% CM was significantly higher than the background number of Eo-type colonies (negative control). Comparison of Gm CSA among 1%, 5% and 10% CM with negative, revealed no significant differences. We also compared the Eo-type CSA in the presence of 10% significant differences. We also compared the Eo-type CSA in the presence of 10% CM in the 14-day methylcellulose assay with 21-day methylcellulose assay. There was no significant difference in the number of Eo-type colony between the 14-day and 21-day methylcellulose assays. We also examined the composition of the cells in the colonies in the 14-day and 21-day methylcellulose cultures. The percentage of metachromatic granule containing cells in a Eo-type colony in 14-day methylcellulose assay was significantly higher than in a Eo-type colony in 21-day methylcellulose assay.(ABSTRACT TRUNCATED AT 250 WORDS)
Ohnishi
M
M
Department of Otorhinolaryngology, Nippon Medical School, Tokyo.
Pawankar
R
R
Otsuka
H
H
Ikeda
M
M
Fujikura
T
T
Okuda
M
M
D000328:Adult; D001491:Basophils / Q000473:pathology*; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000378:metabolism*; D003470:Culture Media; D004804:Eosinophils / Q000473:pathology*; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005260:Female; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000473:pathology*; D009297:Nasal Mucosa / Q000378:metabolism* / Q000473:pathology; D012221:Rhinitis, Allergic, Perennial / Q000378:metabolism* / Q000473:pathology
D003115:Colony-Stimulating Factors; D003470:Culture Media