Plasma progesterone, luteinizing hormone concentrations, and granulosa cell responsiveness in heat-stressed hens.
Plasma progesterone and luteinizing hormone (LH) profiles were obtained during the first ovulatory cycle of heat-stressed (HS, 35 C; n = 24) and unstressed (US, 17 to 27 C; n = 24) hens using 30-min sampling intervals beginning approximately 6 h prior to ovulation. Progesterone levels from HS hens were lower from 6 h [.07 +/- .01 (SE) versus 1.66 +/- .25 ng/mL; P = .008] to predicted ovulation (.06 +/- .006 versus .70 +/- .18 ng/mL; P = .07). Likewise, LH levels from HS hens were lower from 6 h (1.55 +/- .16 versus 3.86 +/- .34 ng/mL; P = .007) to predicted ovulation (1.63 +/- .18 versus 2.50 +/- .27 ng/mL; P = .01). Eggs from HS hens were more often laid early (less than 24 h) than eggs from US hens (71.42 versus 13.33%, respectively; P = .01), but US hens more often laid eggs of a normal oviposition interval length (24 to 26 h) compared with HS hens (73.34 versus 14.29%; P = .0005). The percentage of delayed eggs (greater than 26 h) was not different (US, 14.29 versus HS, 13.37%; P = .75) between the two treatment groups. Basal production of progesterone by dispersed granulosa cells from US hens was 97.62 +/- 16.01 ng/mL. Challenge by LH increased this to 417.50 +/- 53.38 ng/mL (P = .0001). In contrast, basal progesterone secretion by cells from HS hens was 40.25 +/- 6.60 ng/mL (P = .0001) and LH challenge failed to increase progesterone production.(ABSTRACT TRUNCATED AT 250 WORDS)
Novero
R P
RP
Department of Animal Sciences, University of Nebraska-Lincoln 68583-0908.
Beck
M M
MM
Gleaves
E W
EW
Johnson
A L
AL
Deshazer
J A
JA
D000818:Animals; D002645:Chickens; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism; D006358:Hot Temperature / Q000009:adverse effects; D007986:Luteinizing Hormone / Q000097:blood*; D010060:Ovulation; D011201:Poultry Diseases / Q000097:blood* / Q000503:physiopathology; D011374:Progesterone / Q000097:blood*; D013312:Stress, Physiological / Q000097:blood / Q000503:physiopathology / Q000662:veterinary*
D011374:Progesterone; D007986:Luteinizing Hormone