Anterograde neuroanatomical tract tracing with central nervous system injections of immunoglobulin G: a light and electron microscopic evaluation.
Normal rabbit serum (NRS) was pressure injected into the forebrain of rats to be tested as an anterograde neuroanatomical tracing substance. Undiluted NRS was stereotaxically injected into the bed nucleus of the stria terminalis (BST) with a 1-microliter Hamilton syringe. Postinjection survival times ranged from 24 h to 14 days. An immunohistochemical method utilizing goat anti-rabbit IgG antibody was used to detect the rabbit IgG within vibratome sections. Visualization of the final reaction product (diaminobenzidine, DAB) was enhanced by a silver/gold postintensification (SGI) method. Rabbit IgG-containing neural structures were examined at both light and electron microscopic (EM) levels. At the injection site neuronal soma, dendrites and axons were filled homogeneously with the SGI-DAB at 24 h, 48 h, 7 days and 14 days indicating local neuronal uptake, storage and transport of rabbit IgG. In the hypothalamus many anterogradely filled axons were present and displayed short collateral branches and terminals. EM examination revealed synaptic terminals containing IgG, without signs of transsynaptic transport after 14 days. Signs of retrograde transport of IgG were never observed. A propensity of neurons to take up, sequester and anterogradely transport immunoglobulin G is indicated.
Phelix
C F
CF
Division of Life Sciences, University of Texas, San Antonio 78285.
Paull
W K
WK
D000818:Animals; D001692:Biological Transport; D007031:Hypothalamus / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000378:metabolism*; D007150:Immunohistochemistry; D007277:Injections, Jet; D008297:Male; D008854:Microscopy, Electron; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013787:Thalamic Nuclei / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure
D007074:Immunoglobulin G