title: A gene cluster required for coordinated biosynthesis of lipopolysaccharide and extracellular polysaccharide also affects virulence of Pseudomonas solanacearum.
abstract: Bacterial cell surface components can be important determinants of virulence. At least three gene clusters important for extracellular polysaccharide (EPS) biosynthesis have been previously identified in the plant pathogen Pseudomonas solanacearum. We have found that one of these gene clusters, named ops, is also required for lipopolysaccharide (LPS) biosynthesis. Mutations in any complementation unit of this cluster decreased EPS production, prevented the binding of an LPS-specific phage, and altered the mobility of purified LPS in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. However, restoration of LPS biosynthesis alone was not sufficient to restore virulence to the wild-type level, suggesting that EPS is important for pathogenesis.
authors.affiliation: Department of Plant Pathology, University of Wisconsin, Madison 53706.
authors.forename: C C
authors.initials: CC
authors.lastname: Kao
authors.forename: L
authors.initials: L
authors.lastname: Sequeira
mesh_terms: D001435:Bacteriophages / Q000378:metabolism; D005816:Genetic Complementation Test; D008070:Lipopolysaccharides / Q000096:biosynthesis / Q000235:genetics*; D005810:Multigene Family; D009154:Mutation; D010944:Plants / Q000382:microbiology; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D011549:Pseudomonas / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
chemical_list: D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial