title: Expression of perforin by natural killer cells within first trimester endometrium in humans.
abstract: The lymphocyte pore-forming protein (PFP)--perforin, also named cytolysin--is a potent mediator of cytotoxicity found in the granules of cytotoxic T lymphocytes and natural killer (NK) cells. Granulated metrial gland (GMG) cells found in the pregnant mouse uterus express perforin and are thought to be highly activated cytolytic lymphocytes related to NK cells. Their role in pregnancy is unknown. Human endometrial granulocytes (EGs) are phenotypically similar to murine GMG cells and, like them, express NK cell markers. However, up to now it was not known whether EGs also express perforin. By means of immunohistochemical analysis, using antisera specific for perforin and monoclonal antibodies to CD56 (NKH-1), CD2 (T11), CD3 (Leu-4), CD4 (Leu-3a), and CD8 (OKT-8), we demonstrated that perforin is present in EGs in the decidualized endometrial stroma and decidual tissue of first-trimester gestational endometrium. In fact, double immunohistochemical labeling demonstrated the co-expression of perforin and NKH-1. This population also expressed some T-cell surface antigens (Leu-4 and T11), but not Leu-3a or OKT-8. Chorionic villi, in contrast, lack perforin+ cells. The presence of a potent cytolytic mediator in NK-like cells in both murine and human pregnant uterus raises the issue of the function of such cells in pregnancy.
authors.affiliation: Department of Pathology, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan.
authors.forename: P Y
authors.initials: PY
authors.lastname: Lin
authors.forename: S V
authors.initials: SV
authors.lastname: Joag
authors.forename: J D
authors.initials: JD
authors.lastname: Young
authors.forename: Y S
authors.initials: YS
authors.lastname: Chang
authors.forename: Y K
authors.initials: YK
authors.lastname: Soong
authors.forename: T T
authors.initials: TT
authors.lastname: Kuo
mesh_terms: D000328:Adult; D004717:Endometrium / Q000166:cytology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007694:Killer Cells, Natural / Q000737:chemistry*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000032:analysis*; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D011247:Pregnancy; D011261:Pregnancy Trimester, First
chemical_list: D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D052899:Pore Forming Cytotoxic Proteins; D054353:Perforin