[A new method for immunological detection of fecal blood, using colloidal gold labeled with anti-human hemoglobin monoclonal antibody].
Application of colloidal gold agglutination to detection of fecal blood was done. Colloidal gold labeled with monoclonal antibody recognized human hemoglobin was agglutinated with hemoglobin and changed the color from red to gray. The color that diminished rapidly with an antigen was unchangeable from 30 min to 4 hr. Reaction was single step, and positive tests were easily distinguished from negative tests. Detectable hemoglobin concentration was 0.5 micrograms/ml. Comparison of colloidal gold agglutination with other methods, as low as latex agglutination and RPHA, showed good agreements in 1409 fecal samples. Colloidal gold agglutination method is superior in easiness of judgement and unnecessity of keeping time, and will be suitable for mass screening for colorectal cancer using 96 well microplate.
Narimatu
S
S
Department of Clinical Chemistry and Laboratory Medicine, Fukuoka University Hospital.
Nagata
M
M
Sugizaki
S
S
Hamasaki
N
N
D000372:Agglutination Tests / Q000379:methods*; D000911:Antibodies, Monoclonal / Q000276:immunology; D003102:Colloids; D015179:Colorectal Neoplasms / Q000175:diagnosis; D006046:Gold; D006454:Hemoglobins / Q000276:immunology*; D006801:Humans; D009780:Occult Blood; D011237:Predictive Value of Tests
D000911:Antibodies, Monoclonal; D003102:Colloids; D006454:Hemoglobins; D006046:Gold