title: TFS1: a suppressor of cdc25 mutations in Saccharomyces cerevisiae.
abstract: The TFS1 gene of Saccharomyces cerevisiae is a dosage-dependent suppressor of cdc25 mutations. Overexpression of TFS1 does not alleviate defects of temperature-sensitive adenylyl cyclase (cdc35) or ras2 disruption mutations. The ability of TFS1 to suppress cdc25 is allele specific: the temperature-sensitive cdc25-1 mutation is suppressed efficiently but the cdc25-5 mutation and two disruption mutations are only partially suppressed. TFS1 maps to a previously undefined locus on chromosome XII between RDN1 and CDC42. The DNA sequence of TFS1 contains a single long open reading frame encoding a 219 amino acid polypeptide that is similar in sequence to two mammalian brain proteins. Insertion and deletion mutations in TFS1 are haploviable, indicating that TFS1 is not essential for growth.
authors.affiliation: Laboratory of Molecular Biology, University of Wisconsin, Madison 53706.
authors.forename: L C
authors.initials: LC
authors.lastname: Robinson
authors.forename: K
authors.initials: K
authors.lastname: Tatchell
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D018797:Cell Cycle Proteins; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D004271:DNA, Fungal; D005656:Fungal Proteins / Q000235:genetics*; D015870:Gene Expression; D005800:Genes, Fungal; D016153:Genes, Suppressor; D011905:Genes, ras; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012331:RNA, Fungal / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D016415:Sequence Alignment; D020666:ras-GRF1
chemical_list: D018797:Cell Cycle Proteins; D004271:DNA, Fungal; D005656:Fungal Proteins; D012331:RNA, Fungal; D020666:ras-GRF1; D000262:Adenylyl Cyclases