title: Separation of beta-casein A1, A2, and B using cation-exchange fast protein liquid chromatography.
abstract: beta-Casein genetic variants A1, A2, and B were separated using cation-exchange fast protein liquid chromatography. beta-Casein from a herd bulk casein sample eluted as a series of three peaks. Casein samples from individual cows containing known combinations of beta-casein A1, A2, and B were used to confirm that the three peaks were beta-casein genetic variants. An acid-PAGE gel confirmed the identity of the peaks that eluted from the column.
authors.affiliation: Western Dairy Foods Research Center Nutrition, Utah State University, Logan 84322-8700.
authors.forename: C M
authors.initials: CM
authors.lastname: Hollar
authors.forename: A J
authors.initials: AJ
authors.lastname: Law
authors.forename: D G
authors.initials: DG
authors.lastname: Dalgleish
authors.forename: J F
authors.initials: JF
authors.lastname: Medrano
authors.forename: R J
authors.initials: RJ
authors.lastname: Brown
mesh_terms: D000818:Animals; D002364:Caseins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008892:Milk / Q000032:analysis*
chemical_list: D002364:Caseins