[Identification of a type 1 macrocreatine kinase].
A macrocreatine-kinase type 1 was identified by CK isoenzyme electrophoresis of a serum sample from a patient. Constituents of this enzyme complex were studied. The isoenzyme present in the macrocreatine-kinase was identified using immunoprecipitation with antibodies against the M subunit. Affinity chromatography then determined the type of the immunoglobulin bound to the CK isoenzyme. A protocol for studying macrocreatine-kinase type 1 compounds is proposed.
Legras
B
B
Laboratoire de Biochemie A, Hôpital Sud, Rennes, France.
Lemarchand
I
I
Ruelland
A
A
Gallou
G
G
Cloarec
L
L
D002846:Chromatography, Affinity; D003402:Creatine Kinase / Q000097:blood / Q000737:chemistry* / Q000276:immunology; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D007527:Isoenzymes
D007074:Immunoglobulin G; D007527:Isoenzymes; D003402:Creatine Kinase
Identification d'une macrocréatine-kinase type 1.