Development of a 3-D artificial extracellular matrix. Design concept and artificial vascular media.
A photodimerizable monomer was synthesized and subsequently co-polymerized with a hydrophilic monomer to obtain a photocrosslinkable, water soluble polymer. This polymer attained a highly hydrated, gel-like phase on irradiation with ultraviolet (UV) light. Furthermore, incorporation of the well known cell adhesive ligand Arg-Gly-Asp-Ser (RGDS) peptidyl moiety into the above co-polymer resulted, on UV light irradiation, in a three-dimensional hydrophilic gel matrix containing cell adhesive ligands. The UV irradiation on a mixture of photocrosslinkable and cell adhesive polymer and smooth muscle cells in a medium trapped the cells throughout the gel matrix, indicating that the cell-entrapped gel and the designed synthetic polymer biomimic the media of the vascular wall and extracellular matrix, respectively.
Moghaddam
M J
MJ
Department of Bioengineering, National Cardiovascular Center, Osaka, Japan.
Matsuda
T
T
D002454:Cell Differentiation; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000737:chemistry*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D009842:Oligopeptides / Q000737:chemistry*; D011092:Polyethylene Glycols; D011108:Polymers; D014466:Ultraviolet Rays
D009842:Oligopeptides; D011108:Polymers; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; C046617:arginyl-glycyl-aspartyl-serine