PCR using a thermostable polymerase with 3' to 5' exonuclease activity generates blunt products suitable for direct cloning.
Lohff
C J
CJ
Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109-0724.
Cease
K B
KB
D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000378:metabolism; D019914:Taq Polymerase
D004247:DNA; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase