The pattern of plant annexin gene expression.
Peptide sequence data derived from a plant annexin, P34 [Smallwood, Keen & Bowles (1990) Biochem. J. 270, 157-161] was used to design amplimers for PCR. A unique fragment of 95 bp, amplified from tomato (Lycopersicon esculertum) genomic DNA, was used in Northern analyses and demonstrated a differential pattern of expression in vegetative tissues of tomato, potato (Solanum tuberosum) and barley (Hordeum vulgare). The tissue-specific abundance of the annexin transcript was found to correlate closely with abundance of annexin protein as revealed by their partial purification and analysis with antisera specific for annexins isolated from tomato suspension-culture cells.
Smallwood
M F
MF
Department of Biochemistry and Molecular Biology, University of Leeds, U.K.
Gurr
S J
SJ
McPherson
M J
MJ
Roberts
K
K
Bowles
D J
DJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D004247:DNA; D015342:DNA Probes; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics
D015342:DNA Probes; D010940:Plant Proteins; D004247:DNA