Thyrotropin-releasing hormone-immunoreactive projections to the dorsal motor nucleus and the nucleus of the solitary tract of the rat.
Thyrotropin-releasing hormone-immunoreactive nerve terminals heavily innervate the dorsal motor nucleus and nucleus of the solitary tract, whereas cell bodies containing thyrotropin-releasing hormone residue most densely in the hypothalamus and raphe nuclei. By using double-labeling techniques accomplished by retrograde transport of Fluoro-Gold following microinjection into the dorsal motor nucleus/nucleus of the solitary tract combined with immunohistochemistry for thyrotropin-releasing hormone, it was demonstrated that thyrotropin-releasing hormone-immunoreactive neurons projecting to the dorsal motor nucleus/nucleus of the solitary tract reside in the nucleus raphe pallidus, nucleus raphe obscurus, and the parapyramidal region of the ventral medulla, but not in the paraventricular nucleus of the hypothalamus. The parapyramidal region includes an area along the ventral surface of the caudal medulla, lateral to the pyramidal tract and inferior olivary nucleus and ventromedial to the lateral reticular nucleus. Varying the position of the Fluoro-Gold injection site revealed a rostral to caudal topographic organization of these raphe and parapyramidal projections.
Lynn
R B
RB
Department of Medicine, Jefferson Medical College, Philadelphia 19107.
Kreider
M S
MS
Miselis
R R
RR
D000818:Animals; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D008526:Medulla Oblongata / Q000737:chemistry / Q000166:cytology* / Q000276:immunology; D009046:Motor Neurons / Q000276:immunology / Q000378:metabolism*; D009411:Nerve Endings / Q000737:chemistry / Q000648:ultrastructure; D009434:Neural Pathways / Q000166:cytology; D009620:Nodose Ganglion / Q000737:chemistry / Q000166:cytology; D010286:Paraventricular Hypothalamic Nucleus / Q000737:chemistry / Q000166:cytology; D011712:Pyramidal Tracts / Q000166:cytology; D011903:Raphe Nuclei / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D013266:Stilbamidines; D013973:Thyrotropin-Releasing Hormone / Q000032:analysis* / Q000276:immunology; D014630:Vagus Nerve / Q000737:chemistry / Q000166:cytology*; D014966:Xanthenes
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D013266:Stilbamidines; D014966:Xanthenes; D013973:Thyrotropin-Releasing Hormone; C034657:Texas red