IL-2 gene transfer in immunotherapy of cancer: local administration of IL-2-activated lymphocytes and X63-m-IL-2 cells constitutively producing IL-2 inhibits growth of plasmacytomas in syngeneic mice.
Peritumoral administration of X63-m-IL-2 cells, transformed by IL-2 cDNA and constitutively producing large quantities of IL-2, was effective in mediating regression of X63-Ag8.653 plasmacytomas transplanted in syngeneic mice. Injection of killer cells with LAK activity substantially enhanced the tumor-inhibitory effect of IL-2-producing cells. In vitro activation of murine bone marrow and spleen cells with IL-2 produced by the X63-m-IL-2 cell line resulted in generation of lymphocytes with oncolytic activity against X63-Ag8.653 plasmacytomas and a variety of other target cell lines. The cytolytic activity of adherent IL-2-activated killer cells (A-LAK) was substantially higher than that of a nonadherent subset. Bone marrow and spleen cells from both healthy control and tumor-bearing mice contained LAK precursors, but the cytolytic activity derived from tumor-bearing mice was lower than that from healthy controls.
Bubeník
J
J
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
Lotzová
E
E
Indrová
M
M
Simová
J
J
Jandlová
T
T
Savary
C A
CA
D000818:Animals; D002461:Cell Line, Transformed / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D016219:Immunotherapy, Adoptive; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics / Q000627:therapeutic use*; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D008213:Lymphocyte Activation; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D010954:Plasmacytoma / Q000628:therapy*; D014162:Transfection; D014185:Transplantation, Isogeneic; D014407:Tumor Cells, Cultured
D007376:Interleukin-2