Large-scale stable opening of supercoiled DNA in response to temperature and supercoiling in (A + T)-rich regions that promote low-salt cruciform extrusion.
We have studied the properties of (A + T)-rich sequences derived from ColE1 that promote cruciform extrusion at low ionic strength in supercoiled plasmids. We compared the chemical reactivity of the sequences in negatively supercoiled DNA (using osmium tetroxide and bromoacetaldehyde) with the results of two-dimensional gel electrophoresis performed under the same conditions. Taken together, the results indicate the occurrence of cooperative helix-coil transitions in the (A + T)-rich DNA at low ionic strength, to form stable, denatured regions. The extent of the open region is a function of temperature and superhelix density, with an additional local destabilization brought about by the presence of cruciform structures. We present a simple statistical mechanical model of the helix-coil transition in the (A + T)-rich DNA, from which we have obtained estimates of the free energy for average base-pair opening of 0.31 kcal mol-1 and that for the formation of a helix-coil junction of 4.9 kcal mol-1, in 45 mM Tris-borate, pH 8.3, 0.5 mM EDTA. The results offer a model for the C-type mechanism of cruciform extrusion. Inverted repeats that are incorporated into the melted region undergo hairpin loop formation below 50 degrees C, and upon closure of the melted region, by reduction of temperature or increased ionic strength, they remain as a fully extruded cruciform structure.
Bowater
R
R
Department of Biochemistry, The University, Dundee, U.K.
Aboul-ela
F
F
Lilley
D M
DM
D000079:Acetaldehyde / Q000031:analogs & derivatives* / Q000494:pharmacology; D000227:Adenine Nucleotides / Q000737:chemistry; D003086:Bacteriocin Plasmids / Q000235:genetics; D001482:Base Composition; D001483:Base Sequence; D004278:DNA, Superhelical / Q000737:chemistry* / Q000187:drug effects; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation / Q000187:drug effects; D009993:Osmium Tetroxide / Q000494:pharmacology*; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D013696:Temperature; D013942:Thymine Nucleotides / Q000737:chemistry
D000227:Adenine Nucleotides; D004278:DNA, Superhelical; D013942:Thymine Nucleotides; C038235:bromoacetaldehyde; D000079:Acetaldehyde; D009993:Osmium Tetroxide