[Activation of phospholipase hydrolysis in the process of oxidative cell damage].
Effect of tert-butyl hydroperoxide toxic action on phospholipase A2 activity and the changes in phospholipid composition from mastocytoma P815 cells were investigated. Oxidative damage of tumor cell membranes was accompanied by the release of arachidonic acid from membrane phospholipids and the accumulation of lysophosphatidylcholine, the product of phospholipase A2 reaction and a potent detergent. Tert-butyl hydroperoxide also increased relative contents of sphingomyelin, phosphatidylserine and phosphatidic acid in tumor cell membranes. It is possible that phospholipase A2 activation and the changes of phospholipid molecular species contents may cause the damage of cell membrane stability.
Kondakova
I V
IV
Borunov
E V
EV
Antipova
E V
EV
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D016923:Cell Death; D002462:Cell Membrane / Q000378:metabolism; D004789:Enzyme Activation; D006868:Hydrolysis; D051379:Mice; D008811:Mice, Inbred DBA; D016877:Oxidants / Q000494:pharmacology*; D010545:Peroxides / Q000494:pharmacology*; D010741:Phospholipases A / Q000187:drug effects*; D054467:Phospholipases A2; D010743:Phospholipids / Q000378:metabolism; D012513:Sarcoma, Experimental / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured; D020122:tert-Butylhydroperoxide
D016877:Oxidants; D010545:Peroxides; D010743:Phospholipids; D016718:Arachidonic Acid; D020122:tert-Butylhydroperoxide; D010741:Phospholipases A; D054467:Phospholipases A2
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