Effect of short-term exercise training on muscle glycogen in resting conditions in rats fed a high fat diet.
It has been reported that exercise training increases muscle glycogen storage in rats fed a high carbohydrate (CHO) diet in resting conditions. The purpose of this study was to examine whether a 3-week swimming training programme would increase muscle glycogen stores in rats fed a high-fat (FAT) diet in resting conditions. Rats were fed either the FAT or CHO diet for 7 days ad libitum, and then were fed regularly twice a day (between 0800 and 0830 hours and 1800 and 1830 hours) for 32 days. During this period of regular feeding, half of the rats in both dietary groups had swimming training for 3 weeks and the other half were sedentary. The rats were not exercised for 48 h before sacrifice. All rats were killed 2 h after their final meal (2030 hours). The glycogen contents in red gastrocnemius muscle, heart and liver were significantly higher in sedentary rats fed the CHO diet than in those fed the FAT diet. Exercise training clearly increased glycogen content in soleus, red gastrocnemius and heart muscle in rats fed the CHO diet. In rats fed the FAT diet, however, training did not increase glycogen content in these muscles or the heart. Exercise training resulted in an 87% increase of total glycogen synthase activity in the gastrocnemius muscle of rats fed the CHO diet. However, this was not observed in rats fed the FAT diet. The total glycogen phosphorylase activity in the gastrocnemius muscle of the rats of both dietary groups was increased approximately twofold by training.(ABSTRACT TRUNCATED AT 250 WORDS)
Saitoh
S
S
Laboratory of Biochemistry of Exercise and Nutrition, University of Tsukuba, Ibaraki, Japan.
Shimomura
Y
Y
Tasaki
Y
Y
Suzuki
M
M
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D002950:Citrate (si)-Synthase / Q000032:analysis; D004040:Dietary Carbohydrates / Q000494:pharmacology*; D004041:Dietary Fats / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D006003:Glycogen / Q000032:analysis* / Q000378:metabolism; D006006:Glycogen Synthase / Q000032:analysis / Q000378:metabolism; D006885:Hydroxybutyrates / Q000097:blood; D007328:Insulin / Q000097:blood; D008099:Liver / Q000737:chemistry; D008297:Male; D009132:Muscles / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D006005:Phosphorylases / Q000032:analysis; D010805:Physical Conditioning, Animal; D051381:Rats; D011919:Rats, Inbred Strains; D012146:Rest / Q000502:physiology*; D013997:Time Factors
D001786:Blood Glucose; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D005230:Fatty Acids, Nonesterified; D006885:Hydroxybutyrates; D007328:Insulin; D006003:Glycogen; D002950:Citrate (si)-Synthase; D006005:Phosphorylases; D006006:Glycogen Synthase