title: [Obtaining human recombinant (serine-17) beta-interferon by the method of oligonucleotide-directed mutagenesis and its expression in Escherichia coli].
abstract: The gene of human mutant (serine-17) fibroblast interferon was isolated with the use of highly efficient oligonucleotide-directed mutagenesis. On the basis of the constructed expression plasmid pPR-IFN Ser17 a strain producing human mutant beta-interferon (VKPM V-4678) was developed. It was shown that the specific activity of the human mutant (serine-17) fibroblast interferon was 1 order of magnitude higher than that of the recombinant interferon which reaches the specific activity of natural fibroblast interferon.
authors.forename: I I
authors.initials: II
authors.lastname: Shekhter
authors.forename: V P
authors.initials: VP
authors.lastname: Beĭko
authors.forename: M T
authors.initials: MT
authors.lastname: Bulenkov
authors.forename: O M
authors.initials: OM
authors.lastname: Khodova
authors.forename: M A
authors.initials: MA
authors.lastname: Kolevatykh
authors.forename: L S
authors.initials: LS
authors.lastname: Izotova
authors.forename: M I
authors.initials: MI
authors.lastname: Lebedeva
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Rudenskiĭ
authors.forename: S I
authors.initials: SI
authors.lastname: Borukhov
authors.forename: V I
authors.initials: VI
authors.lastname: Iurin
mesh_terms: D001709:Biotechnology / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D000068556:Interferon beta-1a; D000068576:Interferon beta-1b; D016899:Interferon-beta / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D016297:Mutagenesis, Site-Directed / Q000235:genetics*
chemical_list: D000068576:Interferon beta-1b; D016899:Interferon-beta; D000068556:Interferon beta-1a
vernacular_title: Poluchenie rekombinantnogo (serin-17) beta-interferona cheloveka metodom oligonukleotidnapravlennogo mutageneza i ego ékspresiia v Escherichia coli.