Use of M13 single-stranded DNA digoxigenin labelled probe for detection of human parvovirus B19 viraemia.
A dot-blot hybridization assay for serum B19 DNA is described using a non-radioactive (digoxigenin) M13 single-stranded DNA probe. The assay is sensitive (0.3 pg of B19 DNA) and has several advantages over similar assays which use radioactive or non-radioactive labelled plasmid probe for the routine detection of B19 DNA in sera.
Prato
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Centro de Diagnostico French, Buenos Aires, Republica Argentina.
Paper
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Morinet
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D001483:Base Sequence; D015342:DNA Probes; D004076:Digoxigenin; D006801:Humans; D008969:Molecular Sequence Data; D016732:Parvovirus B19, Human / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D015342:DNA Probes; D004076:Digoxigenin