Lateral interactions of pig apolipoprotein A-1 with egg yolk phosphatidylcholine and with cholesterol in mixed monolayers at the triolein-saline interface.
Interfacial tensions of egg yolk phosphatidylcholine (PC) and cholesterol monolayers adsorbed at the triolein-saline interface were measured in the presence and absence of pig apolipoprotein A-1 (apoA-1) in the saline phase. In the absence of apoA-1, the adsorptions of PC and cholesterol at the interface from the triolein phase are cooperative, showing large lateral attractive interactions between the PC molecules and the cholesterol molecules in the monolayer. In the presence of apoA-1, the PC adsorption is anti-cooperative, indicating strong lateral attractive interactions between the PC and the apoA-1 molecules, i.e., apparently, repulsive lateral interactions between the PC molecules. On the other hand, lateral interactions of very low magnitude are observed between the cholesterol and apoA-1 molecules in the monolayer. Values of the lateral interaction energy are evaluated from the adsorption data by the Defay-Prigogine-Flory theory of monolayers. The large difference in lateral interaction energy with apoA-1 between PC and cholesterol in a mixed monolayer is discussed in connection with current problems in lipoprotein catabolism: reverse cholesterol transport, alterations in affinity of lipid particles to apoA-1, and formation of high-density lipoproteins and abnormal lipoproteins.
Handa
T
T
Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Saito
H
H
Tanaka
I
I
Kakee
A
A
Tanaka
K
K
Miyajima
K
K
D000327:Adsorption; D000818:Animals; D016632:Apolipoprotein A-I / Q000737:chemistry*; D008076:Cholesterol, HDL / Q000737:chemistry*; D004530:Egg Yolk / Q000737:chemistry; D006801:Humans; D008079:Lipoproteins, VLDL / Q000737:chemistry; D008567:Membranes, Artificial; D010713:Phosphatidylcholines / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013500:Surface Tension; D013552:Swine; D013816:Thermodynamics; D014304:Triolein / Q000737:chemistry*
D016632:Apolipoprotein A-I; D008076:Cholesterol, HDL; D008079:Lipoproteins, VLDL; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D014304:Triolein; D012965:Sodium Chloride