title: Effect of vasopressin and protein kinase C inhibitors on junctional conductance in isolated heart cell pairs.
abstract: The effect of vasopressin on junctional conductance (gj) in isolated heart cell pairs was investigated. The results indicated that vasopressin (60 nM) causes a decrease in gj of 53% (S.E. +/- 5) (n = 10) within 15 sec. of its administration to the bath. The effect of vasopressin was dose-dependent and was suppressed by staurosporine (50 microM)--a potent inhibitor of protein kinase C. Inhibitors of protein kinase C by themselves increased gj. The results indicate that protein kinase C plays an important role in the control of gj in heart muscle.
authors.affiliation: Department of Pharmacology, School of Medicine, University of Puerto Rico, San Juan 00936-5067.
authors.forename: W C
authors.initials: WC
authors.lastname: De Mello
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D004553:Electric Conductivity / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D006352:Heart Ventricles; D007365:Intercellular Junctions / Q000187:drug effects* / Q000502:physiology; D009206:Myocardium / Q000166:cytology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D051381:Rats; D015398:Signal Transduction; D019311:Staurosporine; D014667:Vasopressins / Q000494:pharmacology*
chemical_list: D000470:Alkaloids; D014667:Vasopressins; D011493:Protein Kinase C; D019311:Staurosporine