title: Restorative effect of neurotropin on maturation of bone marrow cells to IL-2-producing T-cells in aging BALB/c mice.
abstract: In a previous paper, we have demonstrated that Neurotropin, a non-protein extract isolated from the inflamed skin of rabbits inoculated with vaccinia virus, restores decreasing immune responses through the recovery of interleukin-2 (IL-2) production in aging BALB/c mice. To clarify the mechanism by which Neurotropin restores IL-2 production, its effect on the recruitment of IL-2-producing T-cells from bone marrow cells was examined using syngenic radiation bone marrow chimeras. Two fundamental lesions in recruiting IL-2-producing T-cells in aging BALB/c mice were demonstrated: (1) a drastic decline of the maturation of bone marrow cells to IL-2-producing T-cells as demonstrated by old----young chimeras; and (2) an environment unable to support bone marrow cell differentiation to IL-2-producing T-cells by young----old chimeras. Neurotropin clearly restored the maturation of bone marrow cells to IL-2-producing T-cells when administered from 13 to 16 month-old mice, whereas the non-complementing environment was not normalized with Neurotropin administration. These results suggest that Neurotropin administration restores IL-2 production through the recovery of the maturation of bone marrow cells to IL-2-producing T-cells, resulting in restoration of in vivo T-cell immune response in aging BALB/c mice.
authors.affiliation: Department of Hematology and Immunology, Nippon Zoki Pharmaceutical Company, Hyogo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yoshii
authors.forename: H
authors.initials: H
authors.lastname: Kawakubo
authors.forename: T
authors.initials: T
authors.lastname: Matsuoka
authors.forename: S
authors.initials: S
authors.lastname: Suehiro
authors.forename: Y
authors.initials: Y
authors.lastname: Yanagihara
authors.forename: S
authors.initials: S
authors.lastname: Negoro
authors.forename: S
authors.initials: S
authors.lastname: Kishimoto
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000375:Aging / Q000276:immunology; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects* / Q000276:immunology; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D005260:Female; D007376:Interleukin-2 / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000494:pharmacology*; D011828:Radiation Chimera; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D007376:Interleukin-2; D011134:Polysaccharides; C010491:neurotropin