A 1H-NMR study of the solution conformation of cyclo(GRGDSPA): conformational effects on the physiological activity.
Solution conformations of cyclo(GRGDSPA) have been analyzed by the use of two-dimensional proton nuclear magnetic resonance spectroscopy and the dynamical simulated annealing calculation. It has been shown that the RGDS segment in cyclo(GRGDSPA) takes a beta-turn conformation. We have concluded that this beta-turn conformation is essential for the physiological activity of cyclo(GRGDSPA).
Mizutani
R
R
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Shimada
I
I
Ueno
Y
Y
Yoda
M
M
Kumagai
H
H
Arata
Y
Y
D000595:Amino Acid Sequence; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000494:pharmacology; D011487:Protein Conformation; D013329:Structure-Activity Relationship
D010456:Peptides, Cyclic; C068733:cyclo(glycyl-arginyl-glycyl-aspartyl-seryl-aspartyl-alanyl); D006859:Hydrogen