title: Dodecyl maltoside detergent improves resolution of hepatic membrane proteins in two-dimensional gels.
abstract: This report describes the incorporation of an alkyl maltoside detergent in two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) sample lysis buffer in order to improve resolution of protein patterns separated by nonequilibrium pH gradient electrophoresis. Membrane-associated proteins with alkaline isoelectric points form horizontal streaks on two-dimensional electrophoretograms when solubilized with conventional nonionic detergent. Dodecyl maltoside enhances protein delipidation during solubilization and improves pattern resolution and protein mobility.
authors.affiliation: Molecular Anatomy Laboratory, Indiana University-Purdue University, Indianapolis, Columbus 47203.
authors.forename: F
authors.initials: F
authors.lastname: Witzmann
authors.forename: B
authors.initials: B
authors.lastname: Jarnot
authors.forename: D
authors.initials: D
authors.lastname: Parker
mesh_terms: D000818:Animals; D003902:Detergents; D015180:Electrophoresis, Gel, Two-Dimensional; D005960:Glucosides; D008565:Membrane Proteins / Q000032:analysis*; D008930:Mitochondria, Liver / Q000737:chemistry*; D051381:Rats
chemical_list: D003902:Detergents; D005960:Glucosides; D008565:Membrane Proteins; C040358:dodecyl maltoside