title: Crystallization and preliminary data for the ferric form of Lucina pectinata hemoglobin I.
abstract: Cytoplasmic monomeric hemoglobin I from the bacteria-harboring gill of the bivalve mollusc Lucina pectinata has been crystallized in a form suitable for atomic resolution X-ray structural investigations. The crystals have been grown at pH 4.8, in 0.05 M-acetate buffer, using 2.6 M-ammonium sulfate as precipitating agent. The crystals belong to the monoclinic space group P2(1), with unit cell constants a = 50.0 A, b = 38.6 A, c = 42.1 A, beta = 107.1 degrees, and contain one molecule (14,000 Mr) in the asymmetric unit. By means of single crystal microspectrophotometry it has been shown that the crystals contain the ferric form of L. pectinata "sulfide reactive" hemoglobin I. On the other hand, by careful control of the buffering medium composition, it has been possible to obtain stable crystals of the deoxy, oxy and sulfide forms of the protein.
authors.affiliation: Dipartimento di Genetica e Microbiologia, Università di Pavia, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Casale
authors.forename: C
authors.initials: C
authors.lastname: Lionetti
authors.forename: A
authors.initials: A
authors.lastname: Coda
authors.forename: A
authors.initials: A
authors.lastname: Merli
authors.forename: P
authors.initials: P
authors.lastname: Ascenzi
authors.forename: J B
authors.initials: JB
authors.lastname: Wittenberg
authors.forename: M
authors.initials: M
authors.lastname: Bolognesi
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D003461:Crystallography; D005290:Ferric Compounds / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D006454:Hemoglobins