[Study of sphingomyelinase activity and sphingomyelin and ceramide levels in comparison with other lipid fractions of the cell nucleus in regenerating rat liver].
The changes in the activity of cell nuclear sphingomyelinase at different times of activation of RNA and DNA syntheses in regenerating rat liver were studied. A comparative analysis of the intranuclear content of sphingomyelin and the primary product of the sphingomyelinase reaction, ceramide, as well as of other classes of phospholipids and neutral lipids, was carried out. The changes in the sphingomyelin and ceramide content in the nuclei during the induction of synthetic processes were more conspicuous as compared with other phospholipid classes. Neutral lipids of cell nuclei were also shown to undergo substantial changes. The mechanism of possible involvement of sphingomyelin and other lipids in the regulation of replication and transcription processes is discussed.
Pushkarev
M Iu
MIu
Borovkova
O V
OV
Alesenko
A V
AV
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002518:Ceramides / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004789:Enzyme Activation; D050356:Lipid Metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008115:Liver Regeneration; D008297:Male; D051381:Rats; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism*; D013109:Sphingomyelins / Q000378:metabolism*
D002518:Ceramides; D013109:Sphingomyelins; D012321:DNA-Directed RNA Polymerases; D004259:DNA-Directed DNA Polymerase; D013108:Sphingomyelin Phosphodiesterase
Izuchenie urovniia aktivnosti sfingomielinazy i soderzhaniia sfingomielina i tseramidov v sravnenii s drugimi fraktsiiami lipidov kletochnogo iadra regeneriruiushcheĭ pecheni krys.