title: Adrenergic and cholinergic regulation of gastric mucus phospholipid secretion.
abstract: The influence of adrenergic and cholinergic agonists on phospholipid secretion in gastric mucosal cells maintained in the presence of [3H]choline was investigated. The secretion of [3H]choline phospholipids over a 30-min period averaged 1.98% of the total cellular labeled phospholipids in the absence of any mediator and was enhanced by the beta-adrenergic agonist isoproterenol to a greater extent than by the cholinergic agonist pilocarpine. A 2-fold increase in phospholipid secretion was achieved with isoproterenol, whereas pilocarpine produced a 1.3-fold increase. The stimulatory effect of isoproterenol was inhibited by alprenolol, and that of pilocarpine by atropine. The phospholipids secreted in response to isoproterenol showed a 30% decrease in lysophosphatidylcholine, whereas a 2.1-fold enrichment in this phospholipid occurred with pilocarpine. The results demonstrate the involvement of neural mediators in the regulation of phospholipid secretion in gastric mucus.
authors.affiliation: Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
authors.forename: S
authors.initials: S
authors.lastname: Sengupta
authors.forename: E
authors.initials: E
authors.lastname: Piotrowski
authors.forename: A
authors.initials: A
authors.lastname: Slomiany
authors.forename: B L
authors.initials: BL
authors.lastname: Slomiany
mesh_terms: D000526:Alprenolol / Q000494:pharmacology; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001337:Autonomic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D010743:Phospholipids / Q000378:metabolism*; D010862:Pilocarpine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D001337:Autonomic Agents; D010743:Phospholipids; D010862:Pilocarpine; D001285:Atropine; D000526:Alprenolol; D007545:Isoproterenol