title: [Application of Y-chromosome specific DNA analysis for detection of mixed hematopoietic chimerism after allogeneic bone marrow transplantation].
abstract: We report a case involving mixed hematopoietic chimerism after an allogeneic bone marrow transplantation (BMT) from a sex mismatched donor. A 31 year-old-man who was diagnosed as having chronic myelogenous leukemia in the accelerated phase received an allogenic BMT from his HLA-identical sister in March, 1989. To determine the mixed chimerism we used the Y-chromosome specific repeated sequence of DNA using a specific probe (PHY 10). The donor's DNA 3.5 kb band appeared in 1-10% of male DNA by Southern blot hybridization in the peripheral blood 21 days after BMT. The Y-chromosome DNA band decreased day by day, and disappeared 110 days after BMT. The Y-chromosome DNA band could be detected, even though few metaphases were obtained immediately after BMT. Thus this method is very sensitive for determining which cells contain the Y-chromosome, and is therefore useful for detecting mixed chimerism after sex-mismatched BMT. Using this method the clinical significance of mixed chimerism can be assessed.
authors.affiliation: Department of Hematology, Shizuoka Saiseikai General Hospital.
authors.forename: S
authors.initials: S
authors.lastname: Banno
authors.forename: M
authors.initials: M
authors.lastname: Nitta
authors.forename: T
authors.initials: T
authors.lastname: Noda
authors.forename: H
authors.initials: H
authors.lastname: Komatsu
authors.forename: S
authors.initials: S
authors.lastname: Iida
authors.forename: K
authors.initials: K
authors.lastname: Takada
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Mitomo
mesh_terms: D000328:Adult; D016026:Bone Marrow Transplantation; D002678:Chimera / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000601:surgery; D008297:Male; D011184:Postoperative Period; D014184:Transplantation, Homologous; D014998:Y Chromosome
chemical_list: D004247:DNA