Investigation of cis-acting sequences regulating expression of the gene encoding yolk protein 3 in Drosophila melanogaster.
The regulatory sequences leading to the ovarian and fat body expression of yolk proteins 1 and 2 (YP1 and 2) of Drosophila melanogaster have been characterised in some detail. These genes (yp1 and yp2) share many enhancer elements, and some important regulatory sequences lie within the coding regions. We have begun to investigate the cis-regulation of the gene encoding yolk protein 3 (yp3). We describe a system for P element transformation using the complete and unaltered yp3 gene rather than reporter genes and describe sequences conferring correct expression in the ovary and carcass.
Liddell
S
S
Institute of Cell and Molecular Biology, Edinburgh, UK.
Bownes
M
M
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002478:Cells, Cultured; D004247:DNA; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D004527:Egg Proteins / Q000096:biosynthesis / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005786:Gene Expression Regulation; D006458:Hemolymph / Q000378:metabolism; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism; D010957:Plasmids; D012045:Regulatory Sequences, Nucleic Acid; D014170:Transformation, Genetic
D029721:Drosophila Proteins; D004527:Egg Proteins; C070799:YP3 protein, Drosophila; D004247:DNA