[Immunocytochemical analysis of interleukin-1 beta production by human monocytes].
IL-1 was localized within the cytoplasm of human blood monocytes by indirect immunofluorescence using polyclonal rabbit antibodies. After LPS stimulation first IL-1-positive cells appeared at 2 hours and maximal intracellular IL-1 concentration was observed at 10-24 hours when nearly 90% monocytes were labeled with subsequent decline at 48 hours. The highest intracellular IL-1 content preceded its maximal level in cell supernatants.
Simbirtsev
A S
AS
Konusova
V G
VG
Ketlinskiĭ
S A
SA
D002478:Cells, Cultured; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000096:biosynthesis*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D011354:Prodigiozan / Q000494:pharmacology; D013268:Stimulation, Chemical; D013997:Time Factors
D007375:Interleukin-1; D011354:Prodigiozan
Immunotsitokhimicheskiĭ analiz produktsii interleĭkina-1 beta monotsitami cheloveka.