Reduced levels of alpha 1 (I) collagen mRNA in cells immortalized by mutant p53 or transformed by ras.
Genes whose expression patterns are altered in a cell line immortalized by mutant p53 were isolated by differential screening of a cDNA library. Levels of alpha 1 (I) collagen mRNA were reduced in the majority of immortalized cell lines which greatly overproduced the transfected mutant p53. This may reflect a co-selection during the establishment of the cell lines, rather than a direct effect of p53 on alpha 1 (I) collagen gene expression. On the other hand, a more direct relationship could be demonstrated between the expression of activated ras and a reduction in alpha 1 (I) collagen mRNA. Such reduction could partially account for the effects of ras on cell shape and cell proliferation.
Eizenberg
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O
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Oren
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M
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D003094:Collagen / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D016158:Genes, p53; D011905:Genes, ras; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D011498:Protein Precursors / Q000235:genetics; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344; D016415:Sequence Alignment; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
D011498:Protein Precursors; D016159:Tumor Suppressor Protein p53; D003094:Collagen; D016283:Proto-Oncogene Proteins p21(ras)