title: Membrane specificity of non-monotonic trends in cation-mediated injury to rapidly frozen phospholipid vesicles.
abstract: Phospholipid vesicles like erythrocyte ghosts [1] have been shown to display trends in freeze-thaw injury with Group I ions which are non-monotonic in nature. That is to say, the relative extent of injury with such ions, measured as calcein release, does not follow a lyotropic series related solely to the hydrated ionic radii of these ions. By incorporation of cholesterol into the vesicles the non-monotonic nature of these trends has been shown to be highly membrane specific. Thus the non-monotonic trends in cation mediated freeze-thaw injury are shown to be independent of bulk solution properties.
authors.affiliation: Department of Pharmacy, Brighton Polytechnic, Moulsecoomb, U.K.
authors.forename: C A
authors.initials: CA
authors.lastname: Brearley
authors.forename: N A
authors.initials: NA
authors.lastname: Hodges
authors.forename: C J
authors.initials: CJ
authors.lastname: Oliff
mesh_terms: D002412:Cations; D005452:Fluoresceins; D005615:Freezing; D008567:Membranes, Artificial; D010743:Phospholipids / Q000737:chemistry*
chemical_list: D002412:Cations; D005452:Fluoresceins; D008567:Membranes, Artificial; D010743:Phospholipids; C007740:fluorexon