Micronuclei as an index of cytogenetic damage: past, present, and future.
The workshop was designed to present what is known about the production of micronuclei, what protocols are now accepted or proposed internationally, what new results have been obtained, and what new methods and protocols are likely to be forthcoming. This report is designed to convey the flavour of the workshop and to provide the essence of the new information. After the workshop an effort was made to determine what single protocol would satisfy the requirements set for the micronucleus test by as many regulatory agencies as possible. The result, reported here, includes the requirements of six regulatory authorities in Canada, the European Economic Community, the Organization for Economic Co-operation and Development, Japan, and the United States.
Heddle
J A
JA
Department of Biology, York University, Toronto, Canada.
Cimino
M C
MC
Hayashi
M
M
Romagna
F
F
Shelby
M D
MD
Tucker
J D
JD
Vanparys
P
P
MacGregor
J T
JT
D000818:Animals; D001854:Bone Marrow Cells; D002170:Canada; D015342:DNA Probes; D004305:Dose-Response Relationship, Drug; D005062:European Union; D005434:Flow Cytometry; D006077:Government Agencies; D007091:Image Processing, Computer-Assisted; D007564:Japan; D008297:Male; D015162:Micronucleus Tests / Q000639:trends; D008938:Mitosis; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity; D014481:United States
D015342:DNA Probes; D009153:Mutagens