A continuously rotating passive agglutination method using rapidly precipitable latex particles and its application.
A new passive agglutination method is reported. Large, very heavy, uniform latex particles coated with antigens or antibodies were mixed with corresponding antibodies or antigens in reacting wells of an appropriate shape and size. Agglutination results were read after 30 min of continuous rotation at a suitable speed. With this simple and rapid continuously rotating passive agglutination method, we could detect anti-dinitrophenol monoclonal antibodies, natural anti-dinitrophenol antibodies, rheumatoid factor, allergic antibody to tick, human chorionic gonadotrophin and human IgE. This method is suitable to detect weak and small quantities of antibodies in a brief time.
Kimura
H
H
Department of Forensic Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Matsuzawa
S
S
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000032:analysis; D006063:Chorionic Gonadotropin / Q000032:analysis*; D006801:Humans; D006967:Hypersensitivity; D007073:Immunoglobulin E / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007202:Indicators and Reagents; D007840:Latex; D007841:Latex Fixation Tests / Q000379:methods*; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D012016:Reference Values; D012217:Rheumatoid Factor / Q000032:analysis*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D007840:Latex; D007073:Immunoglobulin E; D012217:Rheumatoid Factor