Development of an antibody that binds sulfur mustard.
An antibody that binds bis(2-chloroethyl) sulfide (sulfur mustard) was developed. The immunizing antigen was prepared from the hapten 4-(2-chloroethyl)benzoic acid covalently bound to keyhole limpet hemocyanin (KLH). The antibody was monitored by a solid phase enzyme-linked immunosorbent assay (ELISA). The test antigen consisted of a second hapten, 8-chlorocaprylic acid, covalently bound to bovine serum albumin (BSA). The test antigen was absorbed to the wells of 96-well plates. The immunizing and test antigens contain a common chloroethyl moiety. Thiodiglycol, the principal hydrolysis product of sulfur mustard, does not react with the antibody. This antibody, because of its specificity, has the potential to be a valuable tool for mustard research and forensic detection.
Lieske
C N
CN
USAMRICD, Aberdeen Proving Ground, MD 21010.
Klopcic
R S
RS
Gross
C L
CL
Clark
J H
JH
Dolzine
T W
TW
Logan
T P
TP
Meyer
H G
HG
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D000941:Antigens / Q000737:chemistry; D004355:Drug Stability; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006241:Haptens; D007114:Immunization; D015394:Molecular Structure; D009151:Mustard Gas / Q000737:chemistry*; D011817:Rabbits
D000906:Antibodies; D000941:Antigens; D006241:Haptens; D009151:Mustard Gas