LRV1 viral particles in Leishmania guyanensis contain double-stranded or single-stranded RNA.
The 32-nm-diameter spherical viral particles found in the cytoplasm of Leishmania guyanensis CUMC1-1A sediment at 130S and have a buoyant density of approximately 1.4 g/ml in cesium chloride gradients. These particles contain a 5.3-kb double-stranded RNA, while single-stranded RNA that corresponds to the viral positive strand is associated with less-dense particles. These results suggest a conservative and sequential mode of LRV1 viral RNA replication that is exemplified by the ScV L-A virus of yeast.
Weeks
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Seattle Biomedical Research Institute, Washington 98109-1651.
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Myler
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Stuart
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D000818:Animals; D015152:Blotting, Northern; D003593:Cytoplasm / Q000382:microbiology; D007892:Leishmania braziliensis / Q000382:microbiology*; D015347:RNA Probes; D012328:RNA Viruses / Q000737:chemistry*; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*
D015347:RNA Probes; D012330:RNA, Double-Stranded; D012367:RNA, Viral