title: [Identification of a type 1 macrocreatine kinase].
abstract: A macrocreatine-kinase type 1 was identified by CK isoenzyme electrophoresis of a serum sample from a patient. Constituents of this enzyme complex were studied. The isoenzyme present in the macrocreatine-kinase was identified using immunoprecipitation with antibodies against the M subunit. Affinity chromatography then determined the type of the immunoglobulin bound to the CK isoenzyme. A protocol for studying macrocreatine-kinase type 1 compounds is proposed.
authors.affiliation: Laboratoire de Biochemie A, Hôpital Sud, Rennes, France.
authors.forename: B
authors.initials: B
authors.lastname: Legras
authors.forename: I
authors.initials: I
authors.lastname: Lemarchand
authors.forename: A
authors.initials: A
authors.lastname: Ruelland
authors.forename: G
authors.initials: G
authors.lastname: Gallou
authors.forename: L
authors.initials: L
authors.lastname: Cloarec
mesh_terms: D002846:Chromatography, Affinity; D003402:Creatine Kinase / Q000097:blood / Q000737:chemistry* / Q000276:immunology; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D007527:Isoenzymes
chemical_list: D007074:Immunoglobulin G; D007527:Isoenzymes; D003402:Creatine Kinase
vernacular_title: Identification d'une macrocréatine-kinase type 1.