title: Transplanted photoreceptors identified in dystrophic mouse retina by a transgenic reporter gene.
abstract: Dissociated photoreceptor cells from a transgenic strain of mice, containing a bovine promoter lac Z gene construct, were transplanted to the dystrophic retinas of a strain of mice (C3H, rd/rd) without obvious photoreceptors. The transgenic photoreceptor cells expressed beta-galactosidase and were distinguishable from the host retinal cells by light and electron microscopy after the 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside histochemical reaction. These results showed that transplanted transgenic photoreceptor cells survived at least 1 month in the host retina and had a primitive outer segment and a well-developed synaptic terminal.
authors.affiliation: Department of Ophthalmology, E.S. Harkness Eye Institute, Columbia University, New York, New York 10032.
authors.forename: P
authors.initials: P
authors.lastname: Gouras
authors.forename: J
authors.initials: J
authors.lastname: Du
authors.forename: H
authors.initials: H
authors.lastname: Kjeldbye
authors.forename: R
authors.initials: R
authors.lastname: Kwun
authors.forename: R
authors.initials: R
authors.lastname: Lopez
authors.forename: D J
authors.initials: DJ
authors.lastname: Zack
mesh_terms: D000818:Animals; D002470:Cell Survival; D004195:Disease Models, Animal; D005697:Galactosides; D015971:Gene Expression Regulation, Enzymologic; D005819:Genetic Markers; D006651:Histocytochemistry; D007211:Indoles; D007763:Lac Operon / Q000235:genetics*; D051379:Mice; D008809:Mice, Inbred C3H; D008822:Mice, Transgenic; D010786:Photoreceptor Cells / Q000201:enzymology / Q000637:transplantation* / Q000648:ultrastructure; D012162:Retinal Degeneration / Q000378:metabolism / Q000473:pathology; D012243:Rhodopsin / Q000235:genetics; D012374:Rod Cell Outer Segment / Q000648:ultrastructure; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D005697:Galactosides; D005819:Genetic Markers; D007211:Indoles; D012243:Rhodopsin; D001616:beta-Galactosidase; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside