[Transfer of bacteriophage PRD1 genes into modified plasmid Sa of Escherichia coli and Francisella tularensis cells].
The donor specific bacteriophage PRDI has been shown to mediate the genes transfer into Escherichia coli and Francisella tularensis cell under certain conditions. It is necessary for the process that the recipient cells inherit the plasmids determining absorbtion of bacteriophages on the cellular surface while the transferred genes are able to be expressed. The frequencies of the tet-gene transfer from the plasmid pSKFT5 into Escherichia coli and Francisella tularensis 15 cells inheriting the plasmid Sa are, correspondingly, 10(-6) and 10(-7) clones per bacteriophage plaque.
Pomerantsev
A P
AP
Domaradskiĭ
I V
IV
Zhilenkov
E L
EL
Shishkova
N A
NA
D001435:Bacteriophages / Q000235:genetics* / Q000648:ultrastructure; D002876:Chromosomes, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D005604:Francisella tularensis / Q000235:genetics*; D005798:Genes, Bacterial; D005814:Genes, Viral; D008854:Microscopy, Electron; D010957:Plasmids; D014162:Transfection
Perenos genov bakteriofagov PRD1 v modifitsirovannye plazmidoĭ Sa kletki Escherichia coli i Francisella tularensis.