Recovery of Histoplasma capsulatum from blood in a commercial radiometric Mycobacterium medium.
We report the recovery of Histoplasma capsulatum from blood specimens cultured for Mycobacterium sp. in BACTEC 13A radiometric medium. H. capsulatum was recovered from six of eight blood specimens submitted for mycobacterial cultures from five human immunodeficiency virus-positive individuals. Initial positive metabolic signals occurred at a mean of 11 days, but no organisms were detected with acid-fast stains. The bottles remained positive, and after an additional incubation (mean, 8 days), yeast cells morphologically compatible with H. capsulatum were detected when aliquots were stained with acridine orange. Therefore, when radiometric mycobacterial blood cultures with persistent positive metabolic signals and negative acid-fast stains are encountered, acridine orange staining and subculturing for a variety of microorganisms, including fungi, e.g., H. capsulatum, should be considered.
Merz
W G
WG
Department of Laboratory Medicine, Johns Hopkins Medical Institutions, Baltimore, Maryland 21205.
Kodsy
S
S
Merz
C S
CS
D000818:Animals; D003470:Culture Media; D015658:HIV Infections / Q000097:blood / Q000382:microbiology; D006658:Histoplasma / Q000254:growth & development / Q000302:isolation & purification*; D006660:Histoplasmosis / Q000097:blood* / Q000382:microbiology; D009161:Mycobacterium / Q000254:growth & development*; D011874:Radiometry; D012756:Sheep
D003470:Culture Media