title: Hepatocytes enhance neurite regeneration and survival from transected nerve terminals.
abstract: The culture of hepatocytes dissociated from adult mice by the collagenase perfusion method enables neurons or neuronal tissues to be cultured with hepatocytes or in hepatocyte-conditioned media. Co-culture with hepatocytes or hepatocyte-conditioned media enhances neurite regeneration and their survival from nerve-transected terminals of dorsal root ganglia with nerve fibers dissected from adult and aged mice. Hepatocytes secrete a factor which enhances not only neurite regeneration but also neurite survival. Activities of other known neurotrophic factors were not as crucial as those of the hepatocyte-conditioned medium, suggesting that this factor may differ from other trophic ones.
authors.affiliation: Department of Physiology, School of Medicine, Yokohama City University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Horie
authors.forename: N
authors.initials: N
authors.lastname: Fukuda
authors.forename: Y
authors.initials: Y
authors.lastname: Bando
mesh_terms: D000367:Age Factors; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000648:ultrastructure; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006133:Growth Substances / Q000378:metabolism / Q000494:pharmacology*; D006360:Heat-Shock Proteins / Q000378:metabolism / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000494:pharmacology; D009416:Nerve Regeneration / Q000187:drug effects; D009924:Organ Culture Techniques; D013002:Somatomedins / Q000494:pharmacology
chemical_list: D003470:Culture Media; D006133:Growth Substances; D006360:Heat-Shock Proteins; D007328:Insulin; D009414:Nerve Growth Factors; D013002:Somatomedins; D016222:Fibroblast Growth Factor 2