title: Role of laminin in stimulating rapid-onset neurites in NG108-15 cells: relative contribution of attachment and motility responses.
abstract: The present paper analyzed how attachment and motility responses contribute to the initial formation of rapid-onset neurites in undifferentiated NG108-15 cells. Attachment responses played a positive role in stabilizing neurites against slippage, but exerted a negative effect upon active motility--which appears to be the major rate-determining event in initial outgrowth. The role of laminin in this paradigm was elucidated by analyzing the effects of polyglutamate and other agents (bovine serum albumin and polyethylene glycol chain derivatives) which reduced the number of passive substratum attachment sites, but which did not affect cells directly nor affect the binding of laminin to the substratum. Polyglutamate decreased attachment and spreading of cells, yet greatly accelerated the initial laminin-dependent formation of rapid-onset neurites, and greatly enhanced active neurite remodelling and cell translocation as well. Together with data presented in previous studies in this series, these findings indicate that laminin stimulates rapid-onset neurites primarily by stimulating active motility responses. Insofar as rapid-onset neurites appear to provide a valid model of how neuronal growth cones are regulated, it is likely that these findings will be relevant to developing primary neurons as well.
authors.affiliation: Department of Pediatrics, University of Chicago, IL 60637.
authors.forename: N R
authors.initials: NR
authors.lastname: Smalheiser
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D007797:Laminin / Q000502:physiology*; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000648:ultrastructure*; D010196:Pancreatic Elastase / Q000378:metabolism; D011107:Polylysine / Q000378:metabolism
chemical_list: D007797:Laminin; D011107:Polylysine; D010196:Pancreatic Elastase