[Studies of mtDNA of Ustilago maydis. I. Cloning and gene mapping].
This paper covers the following studies of mtDNA of Ustilago maydis. (1) By inserting the Bam HI and Pst I fragments of the mtDNA into the corresponding sites of pBR322, we cloned a unique sequence of 49.6 kb, accounting for 89.3% of the mitochondrial genome (60.7 kb). (2) With heterogenous genes from plants or fungi as probes, we identified seven genes, and mapped them onto the restriction map of the mt DNA. The genes were arranged in such an order: -UmCOB-UmOXII-S-rR NA-UmOXIII-L-rRNA-UmATPase6-UmOXI-. (3) We tried to express the three cloned genes, UmOXII, UmOXIII, and Um-ATPase 6, in E. coli maxcel expression system, but no specific protein was observed.
Feng
G H
GH
Institute of Microbiology, Academia Sinica, Beijing.
Cheng
W
W
Lu
S Y
SY
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004272:DNA, Mitochondrial / Q000235:genetics*; D005800:Genes, Fungal; D014588:Ustilago / Q000235:genetics*
D004272:DNA, Mitochondrial