Crystallization and preliminary X-ray analysis of complexes of peptide inhibitors with human recombinant and mouse submandibular renins.
Inhibitor-complexed crystals of mouse and human renins suitable for X-ray analysis have been prepared. The mouse renin is complexed with a non-hydrolysable decapeptide analogue of rat angiotensinogen containing a hydroxyethylene isostere in place of the scissile bond. The crystals are monoclinic, space group P2(1) with cell dimensions a = 78.3 A, b = 117.8 A, c = 85.9 A, beta = 101.18 degrees containing four molecules per asymmetric unit. The human renin is fully glycosylated and complexed with a tetrapeptide containing norstatine. The complex crystallises in the cubic space group P2(1)3 with a = 143.1 A and has two molecules in the asymmetric unit. The rotation function of the mouse renin complex indicates pseudo 222 symmetry while that of human renin indicates a pseudo 2-fold axis. Full structural analyses of the two complexes are underway.
Badasso
M
M
Department of Crystallography, Birkbeck College, London, U.K.
Frazao
C
C
Sibanda
B L
BL
Dhanaraj
V
V
DeAlwis
C
C
Cooper
J B
JB
Wood
S P
SP
Blundell
T L
TL
Murakami
K
K
Miyazaki
H
H
D000595:Amino Acid Sequence; D000808:Angiotensinogen / Q000031:analogs & derivatives*; D000818:Animals; D003461:Crystallography; D006023:Glycoproteins / Q000737:chemistry*; D006801:Humans; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D012083:Renin / Q000737:chemistry*; D013363:Submandibular Gland / Q000737:chemistry*; D000092502:Renin Inhibitors
D000808:Angiotensinogen; D006023:Glycoproteins; D046911:Macromolecular Substances; D010446:Peptide Fragments; D011994:Recombinant Proteins; D012083:Renin; D000092502:Renin Inhibitors