title: Conserved amino acid residues in ribosome-inactivating proteins from plants.
abstract: The amino acid sequences of eleven RIPs sequenced to date have been compared in the expectation that this would be useful in the location of functionally and/or structurally important sites of these molecules. In addition to several highly conserved hydrophobic amino acids, thirteen absolutely conserved residues have been found in ricin A-chain: Tyr21, Phe24, Arg29, Tyr80, Tyr123, Gly140, Ala165, Glu177, Ala178, Arg180, Glu208, Asn209 and Trp211. The role of these residues as well as of the C-terminal region have been discussed based on the results of chemical and enzymatic modifications, site-directed mutagenesis, and deletion studies.
authors.affiliation: Laboratory of Protein Chemistry and Engineering, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: G
authors.initials: G
authors.lastname: Funatsu
authors.forename: M R
authors.initials: MR
authors.lastname: Islam
authors.forename: Y
authors.initials: Y
authors.lastname: Minami
authors.forename: K
authors.initials: K
authors.lastname: Sung-Sil
authors.forename: M
authors.initials: M
authors.lastname: Kimura
mesh_terms: D000036:Abrin / Q000235:genetics; D000595:Amino Acid Sequence; D001665:Binding Sites; D000922:Immunotoxins; D037102:Lectins / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009699:N-Glycosyl Hydrolases; D037121:Plant Lectins; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D054789:Ribosome Inactivating Proteins, Type 1; D054790:Ribosome Inactivating Proteins, Type 2; D012270:Ribosomes / Q000378:metabolism*; D012276:Ricin / Q000235:genetics; D000076985:Saporins; D012689:Sequence Homology, Nucleic Acid; D015978:Trichosanthin / Q000235:genetics
chemical_list: D000922:Immunotoxins; D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D054790:Ribosome Inactivating Proteins, Type 2; C017707:Ricinus communis agglutinin-1; C063258:luffin-a protein, Luffa cylindrica; C069038:luffin-b protein, Luffa cylindrica; D000036:Abrin; D015978:Trichosanthin; D012276:Ricin; D009699:N-Glycosyl Hydrolases; C059351:MAP protein, Mirabilis jalapa; D000076985:Saporins; C024275:pokeweed antiviral protein