[Electron microscopic study of DNA compactization under the effect of putrescine].
DNA-putrescine complexes were studied by electron-microscopy with the use of protein-free method. The latter gives the opportunity to investigate the interaction of DNA molecules spread on the surface layer of hypophase and the polyamine molecules in the thick layer of hypophase. Polyamine concentration varied from 5 x 10(-4) mM to 5 x 10(-1) mM. Under the low concentration of putrescine the complexes are represented by agglomerations of kinked knobbed fibres 10 to 20 nm thick, consisting of several fibres of duplex DNA. Upon increasing of putrescine concentration from 5 x 10(-4) to 1.5 x 10(-1) mM, the fibres become more thick (up to 25 nm), highly twisted and have the appearance of cylinders. Very often in the composition of complexes, it is possible to encounter the circular structures, which were formed at the expense of intermolecular interaction of different parts of the complex. The circular structures can serve as "embryos" of toroids of different sizes, that is of different degree of saturation with DNA and putrescine. At the concentration of putrescine 5 x 10(-1) mM the complexes have the appearance of toroids and structures on the basis of toroids, cylinders. The scheme of possible transitions of fibres of various thickness is proposed. The regularities of the compactization process, stimulated by polyamines, don't depend on the degree of compactization (the thickness of compacting fibre), that is they are similar for duplex DNA and for the fibres 25 nm thick, consisting of dozens of DNA molecules.
Novikova
E G
EG
Martynkina
L P
LP
Vengerov
Iu Iu
IuIu
D000818:Animals; D002417:Cattle; D004247:DNA / Q000187:drug effects / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes; D011700:Putrescine / Q000494:pharmacology*; D013950:Thymus Gland / Q000378:metabolism
D009692:Nucleic Acid Heteroduplexes; D004247:DNA; D011700:Putrescine
Elektronno-mikroskopicheskoe izuchenie kompaktizatsii DNK pod deĭstviem putrestsina.