Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi Epimastigotes.
The lipopeptidophosphoglycan is the major cell surface glycoconjugate of the epimastigote forms of the parasitic protozoan Trypanosoma cruzi. A detailed partial structure for this molecule has been reported (Previato, J. O., Gorin, P. A. J., Mazurek, M., Xavier, M. T., Fournet, B., Wieruszesk, J. M., and Mendonca-Previato, L. (1990) J. Biol. Chem. 265, 2518-2526). In this study, we complete the primary structure assignments and describe the microheterogeneity found in the lipopeptidophosphoglycan glycan, using a combination of 1H and 31P NMR, fast atom bombardment mass spectrometry, methylation linkage analysis, and exoglycosidase sequencing. The lipopeptidophosphoglycan is a glycosylated inositol-phosphoceramide with striking homology to glycosylphosphatidylinositol membrane anchors found attached to a wide variety of plasma membrane proteins throughout the eukaryotes.
de Lederkremer
R M
RM
Departamento de Quimica Organica, Facultad de Ciencas Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Lima
C
C
Ramirez
M I
MI
Ferguson
M A
MA
Homans
S W
SW
Thomas-Oates
J
J
D000215:Acylation; D000818:Animals; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D010457:Peptidoglycan / Q000737:chemistry* / Q000302:isolation & purification; D010743:Phospholipids / Q000737:chemistry* / Q000302:isolation & purification; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014349:Trypanosoma cruzi / Q000737:chemistry*
D009844:Oligosaccharides; D010457:Peptidoglycan; D010743:Phospholipids; C014643:lipopeptidophosphoglycan