title: [Immunocytochemical analysis of interleukin-1 beta production by human monocytes].
abstract: IL-1 was localized within the cytoplasm of human blood monocytes by indirect immunofluorescence using polyclonal rabbit antibodies. After LPS stimulation first IL-1-positive cells appeared at 2 hours and maximal intracellular IL-1 concentration was observed at 10-24 hours when nearly 90% monocytes were labeled with subsequent decline at 48 hours. The highest intracellular IL-1 content preceded its maximal level in cell supernatants.
authors.forename: A S
authors.initials: AS
authors.lastname: Simbirtsev
authors.forename: V G
authors.initials: VG
authors.lastname: Konusova
authors.forename: S A
authors.initials: SA
authors.lastname: Ketlinskiĭ
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000096:biosynthesis*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D011354:Prodigiozan / Q000494:pharmacology; D013268:Stimulation, Chemical; D013997:Time Factors
chemical_list: D007375:Interleukin-1; D011354:Prodigiozan
vernacular_title: Immunotsitokhimicheskiĭ analiz produktsii interleĭkina-1 beta monotsitami cheloveka.