Evaluation of cellular viability by quantitative autoradiographic study of myocardial uptake of a fatty acid analogue in isoproterenol-induced focal rat heart necrosis.
Previous studies led us to hypothesize that a fatty acid analogue, 15-p-iodophenyl-beta-methyl pentadecanoic acid (IMPPA or BMIPP), which is taken up but not quickly metabolized by heart cells, would be a more suitable tracer of cellular viability than thallium-201. Biodistribution studies of 1-14C-IMPPA in conscious, freely moving rats showed that the concentration ratio of radioactivity in the heart with respect to the blood was about 8 for at least 60 min after intravenous administration, permitting its use as a putative tracer in these conscious, freely moving rats. Thereafter, the myocardial uptake of 14C-IMPPA was studied in isoproterenol-treated rats (daily treatment for 10 days in order to induce cardiac hypertrophy and necrotic foci) with respect to control ones. Comparison of myocardial localizations by quantitative autoradiography of the uptake of 201Tl and 14C-IMPPA with that of triphenyltetrazolium chloride (TTC) staining enabled comparative evaluation of nutritional blood flow, localization and uptake of 14C-IMPPA and necrotic foci size. Distributions of 14C-IMPPA and 201Tl in control rats' hearts were homogeneous, like TTC staining. In infarcted hearts, areas of decreased 14C-IMPPA uptake were nearly the same (100% +/- 5%) as those unstained by TTC. These areas were larger than those showing a decrease in thallium uptake (about 70% +/- 5% of the total scar size). Therefore, IMPPA seems to be a more accurate and sensitive indicator of necrosis localization compared with thallium. It may be a useful agent for assessment of myocardial viability by single photon emission tomography (SPET) imaging.
Humbert
T
T
Université Joseph Fourier, URA CNRS 1287, Grenoble, France.
Luu-Duc
C
C
Comet
M
M
Demenge
P
P
D000818:Animals; D001345:Autoradiography; D002250:Carbon Radioisotopes; D002470:Cell Survival; D005227:Fatty Acids; D006321:Heart / Q000000981:diagnostic imaging; D007462:Iodobenzenes; D007545:Isoproterenol / Q000633:toxicity; D008297:Male; D009203:Myocardial Infarction / Q000139:chemically induced / Q000000981:diagnostic imaging*; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution; D015899:Tomography, Emission-Computed, Single-Photon
D002250:Carbon Radioisotopes; D005227:Fatty Acids; D007462:Iodobenzenes; C049338:iodofiltic acid; D007545:Isoproterenol