title: Quantitative assessment of tumor enhancement by ultrastable lipid-coated microbubbles as a sonographic contrast agent.
abstract: We have previously reported that ultrastable lipid-coated microbubbles make a suitable ultrasonic contrast agent in the brain, causing increased intensity of echoes that persists for many hours. We showed that intravenously administered lipid-coated microbubbles accumulate selectively in rat brain gliomas with echogenicity enhancement for up to 1 hour, allowing visualization of the growing lesions 40% (2 days) earlier than can be seen without contrast. This work is a detailed evaluation of the accumulation of the lipid-coated microbubbles in tumor and the effect of the bubbles on the echogenicity of insonified tumors. Using a lipid-specific stain, we measured and characterized the distribution of the bubbles in the brain and tumor. We showed that on the scan, the enhancement of the tumor is accompanied by a change in the signal-to-noise ratio of the echoes from the tumor. We identified characteristic textural changes associated with contrast-enhanced tumor using spectral analysis.
authors.affiliation: Department of Surgery, University of Connecticut Health Center, Farmington 06032.
authors.forename: R H
authors.initials: RH
authors.lastname: Simon
authors.forename: S Y
authors.initials: SY
authors.lastname: Ho
authors.forename: C R
authors.initials: CR
authors.lastname: Perkins
authors.forename: J S
authors.initials: JS
authors.lastname: D'Arrigo
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D001932:Brain Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D003287:Contrast Media; D004453:Echoencephalography; D008055:Lipids; D051381:Rats
chemical_list: D003287:Contrast Media; D008055:Lipids