Comparison between lactoferrin and subepithelial matrix protein binding in Staphylococcus aureus associated with bovine mastitis.
Staphylococcus aureus strains (n = 100) isolated from bovine mastitis were classified according to the presence of capsular polysaccharide serotype 5 (n = 46), type 8 (n = 26), and non-5/8 (n = 28). Strains from each type were tested for protein interaction in a 125I-labeled ligand binding assay. A majority of type 5 and type 8 strains showed a higher degree of binding to lactoferrin, fibronectin, and IgG than the non-5/8 strains. Fibrinogen binding was low in all serotypes. Most of the type 5 and non-5/8 strains bound less than 10% laminin, whereas type 8 strains bound laminin in the 11 to 20% range. Non-5/8 strains significantly differed from type 5 in lactoferrin, fibronectin, fibrinogen, and IgG and also from type 8 in fibrinogen and IgG binding. The differences in protein binding between type 5 and type 8 were nonsignificant. The degree of lactoferrin binding in all types positively correlated with laminin binding. Lactoferrin and fibrinogen bindings were correlated in type 5 and type 8 strains. Lactoferrin and fibronectin bindings were correlated only in type 5 strains. These data suggest that bovine lactoferrin binding is common and associated with subepithelial matrix protein interactions in certain serotypes of S. aureus.
Naidu
A S
AS
Department of Medical Microbiology, Malmö General Hospital, Sweden.
Forsgren
A
A
Kalfas
S
S
Watts
J L
JL
Fournier
J M
JM
D000818:Animals; D002417:Cattle; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005260:Female; D005340:Fibrinogen / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007781:Lactoferrin / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism; D008414:Mastitis, Bovine / Q000382:microbiology*; D011485:Protein Binding; D012703:Serotyping; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000145:classification / Q000378:metabolism*
D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007074:Immunoglobulin G; D007797:Laminin; D005340:Fibrinogen; D007781:Lactoferrin