Effect of transforming growth factor beta and basic fibroblast growth factor on steroid-impaired healing intestinal wounds.
A longitudinal intestinal wound model in the pig was used to assess the effect of parenteral steroids (betamethasone 12 mg 50 kg-1 intramuscularly twice daily) on breaking load. Steroid treatment significantly decreased the breaking load of wounds in the ileum and colon in comparison with wounds from saline-treated animals. In a further group of animals receiving steroids, paired longitudinal wounds were constructed. One wound of a pair was treated with a local application of transforming growth factor beta (TGF-beta) (5 micrograms per wound) or basic fibroblast growth factor (5 micrograms per wound) in a collagen suspension. The other wound was treated with a collagen suspension alone. Ileal wounds treated with TGF-beta were significantly stronger than collagen-treated controls at 7 days. The steroid-induced impairment of breaking load in intestinal wounds is partially reversed by a local application of TGF-beta in a collagen suspension at the time of surgery.
Slavin
J
J
Department of Surgery, University of Liverpool, UK.
Nash
J R
JR
Kingsnorth
A N
AN
D000818:Animals; D001623:Betamethasone / Q000037:antagonists & inhibitors; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D007422:Intestines / Q000502:physiology / Q000601:surgery*; D013314:Stress, Mechanical; D013552:Swine; D013718:Tensile Strength; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D001623:Betamethasone