title: Long-term monitoring of depolarization-induced exocytosis from adrenal medullary chromaffin cells and pancreatic islet B cells using "perforated patch recording".
abstract: (1) Membrane capacitance measurements using perforated patch recording offer the possibility of studying the process of depolarization-secretion coupling (DSC) in single endocrine cells with unprecedented time resolution and stability. (2) Early results with catecholamine-secreting adrenal chromaffin cells and insulin-secreting pancreatic B cells support longstanding ideas that the Ca(2+)-dependent processes underlying DSC are fundamentally similar to those of nerve terminals. (3) Future experiments using these approaches should prove useful in sorting out those effects of humoral substances that have a predominant effect on excitability and Ca2+ entry from those that affect the secretory process itself.
authors.affiliation: Department of Medicine (Jewish Hospital), Washington University, St. Louis, Missouri 63110.
authors.forename: K D
authors.initials: KD
authors.lastname: Gillis
authors.forename: R Y
authors.initials: RY
authors.lastname: Pun
authors.forename: S
authors.initials: S
authors.lastname: Misler
mesh_terms: D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002395:Catecholamines / Q000378:metabolism; D002417:Cattle; D002462:Cell Membrane / Q000502:physiology; D002838:Chromaffin System / Q000378:metabolism*; D004553:Electric Conductivity; D004594:Electrophysiology; D005089:Exocytosis; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D051381:Rats
chemical_list: D002395:Catecholamines; D007328:Insulin; D002118:Calcium