Cerebral blood flow and metabolic rate in the conscious, freely moving rat: the effects of hypercapnia, and acute ethanol administration.
We propose a simple method that can be used to measure cerebral blood flow (CBF), cerebral oxygen consumption (CMRO2), and cerebral glucose consumption (CMRglu) in the conscious, freely moving rat. The method is based on the classical Kety-Schmidt approach, and uses a chronic cannula in the confluens sinuum. We tested the method by investigating the response of CBF, CMRO2, and CMRglu to hypercapnia and used the approach to investigate the effects of acute alcohol administration. Severe hypercapnia (PaCO2 approximately 80 mmHg) increased the CBF by a factor of 3.5, decreased the CMRO2 by 30%, and had no significant effect on the CMRglu. Under normocapnic conditions moderate blood alcohol levels (100-200 mg%) caused no significant effects on CBF, CMRO2, or CMRglu, but high blood alcohol levels (250-400 mg%) decreased all three parameters by approximately 25%. Under hypercapnic conditions high blood alcohol levels had no effect on CBF, CMRO2, and CMRglu.
Ligeti
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L
National Institute on Alcohol Abuse and Alcoholism, Rockville, Maryland 20852.
Hines
K
K
Dora
E
E
Sinnwell
T
T
Huang
M T
MT
McLaughlin
A C
AC
D000435:Alcoholic Intoxication / Q000503:physiopathology*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001921:Brain / Q000098:blood supply*; D002245:Carbon Dioxide / Q000097:blood*; D004734:Energy Metabolism / Q000187:drug effects* / Q000502:physiology; D000431:Ethanol / Q000493:pharmacokinetics / Q000633:toxicity*; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects / Q000502:physiology; D051381:Rats; D011916:Rats, Inbred F344; D012039:Regional Blood Flow / Q000187:drug effects / Q000502:physiology
D001786:Blood Glucose; D002245:Carbon Dioxide; D000431:Ethanol