Intramuscular accumulation of prostaglandins during static contraction of the cat triceps surae.
We previously demonstrated that muscle afferent endings are sensitized by exogenous prostaglandins during static contraction of skeletal muscle. The purpose of this study was to determine whether 30 s of static hindlimb contraction, induced by electrical stimulation of the cat sciatic nerve, increases the concentration of immunoreactive prostaglandin E2 (iPGE2) and 6-ketoprostaglandin F1 alpha (i6-keto-PGF1 alpha, the stable metabolite of prostaglandin I2) in muscle tissue. In addition, the role of ischemia in augmenting prostanoid production was examined. Gastrocnemius muscle was obtained by freeze-clamping tissue, and prostaglandins were extracted from muscle homogenates and measured by radioimmunoassay. Compared with precontraction values, high-intensity (68% of maximal tension) static contraction elevated gastrocnemius iPGE2 and i6-keto-PGF1 alpha by 45 and 53%, respectively (P less than 0.01). Likewise, when blood flow to the gastrocnemius was attenuated by arterial occlusion during and 2 min before low-intensity contraction (29% maximal tension), the intramuscular iPGE2 concentration was increased by 71% (P less than 0.01). Conversely, low-intensity contraction (30% of maximal tension) and arterial occlusion without contraction did not alter the concentration of either prostanoid. Our findings demonstrate that prostaglandins accumulate in muscle during static contraction. We believe that local muscle ischemia may provide a stimulus for this phenomenon. These prostaglandins therefore are available to sensitize afferent endings responsible for reflex adjustments during static muscle contraction.
Symons
J D
JD
Department of Internal Medicine, University of California, Davis 95616.
Theodossy
S J
SJ
Longhurst
J C
JC
Stebbins
C L
CL
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000344:Afferent Pathways / Q000502:physiology; D000818:Animals; D001157:Arterial Occlusive Diseases / Q000503:physiopathology; D002415:Cats; D015232:Dinoprostone / Q000378:metabolism; D004558:Electric Stimulation; D009119:Muscle Contraction / Q000502:physiology*; D009132:Muscles / Q000098:blood supply / Q000294:innervation / Q000378:metabolism*; D011453:Prostaglandins / Q000378:metabolism*; D012018:Reflex / Q000502:physiology
D011453:Prostaglandins; D015121:6-Ketoprostaglandin F1 alpha; D015232:Dinoprostone