title: [A method of equalizing the level of DNA sequences of varying amount in a heterogeneous DNA mixture].
abstract: A method for the equalization of double-stranded DNA concentrations in the mixture which may be used for equalizing double-stranded cDNA concentrations involves thermal denaturation of the double-stranded DNA mixture followed by reassociation. The initial reassociation rate is Vi = Ki.(single-stranded DNA)2, and by the end of the process the concentrations of the unreassociated molecules for different DNAs should be approximately equal. Using hydroxylapatite chromatography one can separate single-stranded DNAs from double-stranded DNAs and carry out complete single-stranded DNAs reassociation. The new ratio of different double-stranded DNA concentrations would be almost 1.
authors.forename: A A
authors.initials: AA
authors.lastname: Il'ichev
authors.forename: G P
authors.initials: GP
authors.lastname: Kishchenko
authors.forename: V A
authors.initials: VA
authors.lastname: Petrenko
authors.forename: A T
authors.initials: AT
authors.lastname: Puzyrev
mesh_terms: D002846:Chromatography, Affinity; D004247:DNA / Q000737:chemistry*; D010957:Plasmids
chemical_list: D004247:DNA
vernacular_title: Metod vyravnivaniia soderzhaniia posledovatel'nosteĭ raznoĭ kopiĭnosti v geterogennoĭ smesi DNK.