[Transduction of oncogene RAS into antibody-producing hybridoma].
The effect of introduction by means of electroporation of protogene ras into cells of antibody-producing hybridoma was studied. Doubling time (TD) of transduced subclones, in which H-ras gene was expressed, proved to be 1.8-fold shorter as compared to the parent line. Other characteristics of the hybridoma (cloning efficiency, antibody production in vitro, the time of ascitic fluid appearance in mice and antibody titer in vivo) were not changed. Addition of 12-0-tetradecanoylphorbol acetate to the culture did not alter the growth parameters of H-ras transduced subclones.
Belkina
E V
EV
Deriugina
E I
EI
Drize
N I
NI
Olovnikova
N I
NI
Sadovnikova
E Iu
EIu
Chertkov
I L
IL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001709:Biotechnology / Q000379:methods*; D002478:Cells, Cultured; D015870:Gene Expression / Q000235:genetics* / Q000502:physiology; D011905:Genes, ras / Q000235:genetics* / Q000502:physiology; D006825:Hybridomas / Q000166:cytology* / Q000276:immunology; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000473:pathology; D009857:Oncogenes / Q000235:genetics* / Q000502:physiology; D010957:Plasmids / Q000235:genetics; D013154:Spleen / Q000166:cytology; D014161:Transduction, Genetic / Q000235:genetics*
D000911:Antibodies, Monoclonal
Vvedenie onkogena RAS v kletki antitelo-produtsiruiushcheĭ gibridomy.