Liquid chromatographic method for the determination of calcium cyanamide using pre-column derivatization.
A specific and stability-indicating high-performance liquid chromatographic (HPLC) method has been developed for the analysis of calcium cyanamide in bulk material and dosage form. Calcium cyanamide in samples was converted into dansyl cyanamide. A muBondapak C18 column was employed for HPLC with 0.01 M sodium phosphate (pH 6.3)-acetonitrile (75:25, v/v) as the mobile phase. The proposed HPLC method was validated for linearity, specificity, accuracy and reproducibility.
Chen
S
S
Analytical Development, Lederle Laboratories, Pearl River, NY 10965.
Ocampo
A P
AP
Kucera
P J
PJ
D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D003484:Cyanamide / Q000032:analysis*; D003619:Dansyl Compounds; D004355:Drug Stability; D013053:Spectrophotometry
D003619:Dansyl Compounds; D003484:Cyanamide