Measurements of tissue sorbitol in diabetes mellitus: enzyme method versus gas-liquid chromatography.
Two methods are commonly used to measure sorbitol in mammalian tissues. The first uses sorbitol dehydrogenase for a coupled enzymatic reaction; unfortunately, other polyols are also substrates for this enzyme. The second uses gas-liquid chromatography (GLC) for separation of polyols and mass quantitation of sorbitol. A comparison of these two methods for the measurement of sorbitol in duplicate samples of lens, nerve, and erythrocytes indicates that GLC of polyol acetates consistently finds less sorbitol than measured by sorbitol dehydrogenase. Erythritol, threitol, ribitol, arabitol, and galactitol are polyols found in variable quantities in these tissues, which have a variable influence on the activity of sorbitol dehydrogenase and therefore alter sorbitol quantitation with this enzyme. Moreover, there is an unidentified substance(s) that reacts with sorbitol dehydrogenase which seems to increase in association with hyperglycemia in the lens and nerve, but not in erythrocytes. The quantity of this unknown substance(s) seems to be reduced by the aldose reductase inhibitor sorbinil in erythrocytes and to a lesser extent sciatic nerve and lens. Since enzymatic sorbitol quantitation in the lens, nerve, and erythrocytes is influenced by many known and unknown factors other than sorbitol, we recommend that GLC of polyol acetates be used to measure sorbitol in biologic tissues.
Malone
J I
JI
Department of Pediatrics, University of South Florida College of Medicine, Tampa.
Lowitt
S
S
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors; D000704:Analysis of Variance; D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D003920:Diabetes Mellitus / Q000097:blood*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D006943:Hyperglycemia / Q000097:blood; D007093:Imidazoles / Q000494:pharmacology; D048289:Imidazolidines; D007064:L-Iditol 2-Dehydrogenase; D007908:Lens, Crystalline / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000187:drug effects / Q000378:metabolism*; D013012:Sorbitol / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods; D013402:Sugar Alcohols / Q000378:metabolism
D007093:Imidazoles; D048289:Imidazolidines; D013402:Sugar Alcohols; D013012:Sorbitol; D007064:L-Iditol 2-Dehydrogenase; D000449:Aldehyde Reductase; C026411:sorbinil