Mycobacterium paratuberculosis antigen D: characterization and evidence that it is a bacterioferritin.
By using a combination of agarose and polyacrylamide gel electrophoresis, Mycobacterium paratuberculosis antigen D was resolved from a crude sonicated preparation of the organism and characterized as a component with a molecular mass of approximately 400,000 Da. While this component was composed mainly of protein, with unusually high proportions of glutamic acid and leucine, it was resistant to digestion with a number of proteolytic enzymes. Structural detail revealed by electron microscopy, amino acid sequence data, and the demonstration of a Soret band in its absorption spectrum indicated that antigen D was similar to an Escherichia coli bacterioferritin.
Brooks
B W
BW
Agriculture Canada, Animal Diseases Research Institute, Nepean, Ontario, Canada.
Young
N M
NM
Watson
D C
DC
Robertson
R H
RH
Sugden
E A
EA
Nielsen
K H
KH
Becker
S A
SA
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins; D003573:Cytochrome b Group / Q000032:analysis / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005293:Ferritins / Q000032:analysis / Q000235:genetics; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology*; D012689:Sequence Homology, Nucleic Acid; D013056:Spectrophotometry, Ultraviolet
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003573:Cytochrome b Group; C072165:antigen D, Mycobacterium paratuberculosis; D005293:Ferritins; C018032:bacterioferritin