Primary structure of alpaca growth hormone.
Reduced and carbamidomethylated alpaca growth hormone was submitted to tryptic digestion. Peptides in the mixture were purified by reverse phase HPLC and N-terminal determination and an amino acid analysis of each was performed. Data obtained and the already known primary structure of the equine growth hormone allowed the assembly-by homology-of a definite sequence of amino acids for the polypeptide chain of the protein. Present data provide further information about the relationship between growth factors.
de Jiménez Bonino
M B
MB
Institute of Chemistry and Biological Physicochemistry (UBA-CONICET), Faculty of Pharmacy and Biochemistry, Buenos Aires, Argentina.
de Nué
I A
IA
Oré
R
R
Sánchez
D
D
Ferrara
P
P
Capdevielle
J
J
Cascone
O
O
D000595:Amino Acid Sequence; D000818:Animals; D002161:Camelids, New World / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D013006:Growth Hormone / Q000737:chemistry* / Q000235:genetics*; D007460:Iodoacetamide / Q000737:chemistry; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D013439:Sulfhydryl Reagents; D014357:Trypsin / Q000378:metabolism
D013439:Sulfhydryl Reagents; D013006:Growth Hormone; D014357:Trypsin; D007460:Iodoacetamide