title: Inhibition of the interaction between lipoproteins and amphotericin B by some delivery systems.
abstract: Amphotericin B (AmB) is a potent antifungal agent used to treat patients with systemic mycoses. The clinical usefulness of the drug is limited by its high toxicity and several new less toxic formulations of AmB have been recently developed. In order to understand the mechanism of the decreases of toxicity caused by various new delivery systems, we have investigated by uv-visible spectroscopy the interaction of two of these formulations with human blood lipoproteins. The results were compared with those obtained with the commonly used pharmaceutical form of AmB (Fungizone). This study shows that AmB-lipoprotein interaction is hindered when the drug is in a monomeric form and/or when it is included in phospholipid-surfactant micelles. In an in vivo study on mice it is shown here that AmB monomerized by surfactant is less toxic to animals than the same concentration of Fungizone, where the polyene is strongly aggregated. It may be concluded from the present study that the AmB species which is responsible for the in vivo toxicity is a complex of the antibiotic with the low density and the very low density blood lipoproteins and that hindering of this complex formation results in a decrease of AmB toxicity.
authors.affiliation: Département de chimie-biologie, Université du Québec à Trois-Rivières, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Barwicz
authors.forename: R
authors.initials: R
authors.lastname: Gareau
authors.forename: A
authors.initials: A
authors.lastname: Audet
authors.forename: A
authors.initials: A
authors.lastname: Morisset
authors.forename: J
authors.initials: J
authors.lastname: Villiard
authors.forename: I
authors.initials: I
authors.lastname: Gruda
mesh_terms: D000666:Amphotericin B / Q000008:administration & dosage* / Q000097:blood / Q000737:chemistry; D000818:Animals; D004337:Drug Carriers; D004347:Drug Interactions; D005260:Female; D008074:Lipoproteins / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D046911:Macromolecular Substances; D051379:Mice; D008807:Mice, Inbred BALB C; D008968:Molecular Conformation; D013053:Spectrophotometry
chemical_list: D004337:Drug Carriers; D008074:Lipoproteins; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D046911:Macromolecular Substances; D000666:Amphotericin B