Brief communication. on the problem of immunological detection of antigens in skeletal remains.
A detailed investigation with affinity-chromatography-purified fractions of antihemoglobin serum from rabbit shows that the hemoglobin content of human bones dating back 15 to 3,000 years may be very small. Some of the previous results (Ascenzi et al., 1985) indicating a high hemoglobin titer were +vitiated because of an unexpected cross-reactivity of bone extracts with the hemoglobin-unreactive fraction of the antiserum.
Lendaro
E
E
Department of Biochemical Sciences, University of Rome La Sapienza, Italy.
Ippoliti
R
R
Bellelli
A
A
Brunori
M
M
Zito
R
R
Citro
G
G
Ascenzi
A
A
D000818:Animals; D000941:Antigens / Q000032:analysis*; D001842:Bone and Bones / Q000737:chemistry* / Q000276:immunology; D002846:Chromatography, Affinity; D003429:Cross Reactions; D005189:False Positive Reactions; D006454:Hemoglobins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D015151:Immunoblotting; D007136:Immunoglobulins / Q000276:immunology*; D008159:Lumbar Vertebrae / Q000737:chemistry; D011817:Rabbits; D012886:Skull / Q000737:chemistry; D013997:Time Factors
D000941:Antigens; D006454:Hemoglobins; D007106:Immune Sera; D007136:Immunoglobulins