On the conformation of the alpha sarcin stem-loop of 28S rRNA.
A synthetic RNA that is a substrate for the cytotoxin alpha sarcin has been examined by NMR. The molecule in question includes the entire sequence of the so-called alpha sarcin loop from rat 28S rRNA (U4316-C4332), and it is cleaved at the residue that corresponds to G4325, the site of alpha sarcin cleavage in 28S rRNA. The data show that the terminal stem designed into the molecule's sequence exists, as expected, and that its loop has a definite structure, which is stable to at least 40 degrees C under ionic conditions compatible with its cleavage by alpha sarcin.
Szewczak
A A
AA
Department of Chemistry, Yale University, New Haven, CT 06511.
Chan
Y L
YL
Moore
P B
PB
Wool
I G
IG
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004722:Endoribonucleases; D005656:Fungal Proteins / Q000378:metabolism*; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012339:RNA, Ribosomal, 28S / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D013816:Thermodynamics
D005656:Fungal Proteins; D012339:RNA, Ribosomal, 28S; C014801:alpha-sarcin; D004722:Endoribonucleases