title: Capillary electrophoresis of DNA in entangled polymer solutions.
abstract: Electrophoretic separations of DNA restriction fragments were performed in solutions of hydroxyethylcellulose (HEC) using capillary electrophoresis. Rheological studies confirmed that the entanglement threshold (phi*) for the solution is ca. 0.003 g/ml, in good agreement with theoretical predictions. A mesh size an order of magnitude smaller than that found in agarose gels was calculated using polymer-entanglement theory and was confirmed by electrophoretic measurements. Electrophoretic migration was shown to follow the Ogston regime under most conditions. An approach for obtaining smaller mesh sizes is presented.
authors.affiliation: Department of Chemical Engineering, University of California, Berkeley 94720.
authors.forename: P D
authors.initials: PD
authors.lastname: Grossman
authors.forename: D S
authors.initials: DS
authors.lastname: Soane
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis*; D004586:Electrophoresis / Q000379:methods*; D011108:Polymers
chemical_list: D004269:DNA, Bacterial; D011108:Polymers