[Features of the interaction of Escherichia coli and Francisella tularensis RNA polymerases with hybrid plasmids bearing fragments of Francisella tularensis chromosomal DNA].
Hybrid plasmids containing the fragments of Francisella tularensis chromosomal DNA and capable of tet-gene expression both in Escherichia coli and Francisella tularensis cells were constructed. The regions of francisella chromosomal DNA binding the RNA-polymerases of Escherichia coli and Francisella tularensis were found by the electron microscopy technique. Interconnection of those regions with the expression of tet-gene of the hybrid plasmids was demonstrated.
Pomerantsev
A P
AP
Domaradskiĭ
I V
IV
Doronin
I P
IP
Ershov
Iu V
IuV
D002850:Chromatography, Gel; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000378:metabolism* / Q000648:ultrastructure; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism* / Q000648:ultrastructure; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005604:Francisella tularensis / Q000201:enzymology / Q000235:genetics*; D008854:Microscopy, Electron; D010957:Plasmids
D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases
Osobennosti vzaimodeĭstviia RNK-polimeraz Escherichia coli i Francisella tularensis s gibridnymi plazmidami, nesushchimi fragmenty khromosomnoĭ DNK Francisella tularensis.