title: Inhibition of PGE2 production in macrophages from vitamin E-treated rats.
abstract: Rat peritoneal macrophages from vitamin E-treated rats (5 mg per rat for 6 successive days) contained 403.3 +/- 90.7 ng alpha-tocopherol/10(6) cells, whereas control macrophages contained 1.2 +/- 0.4 ng. PGE2 production in the macrophages from vitamin E-treated rats was significantly suppressed when stimulated with PMA and calcium ionophore A23187. The mechanism of vitamin E inhibition of PGE2 production in macrophages was investigated. The release of (14C)-arachidonic acid from pre-labeled macrophages and the conversion of (14C)-arachidonic acid to PGE2 by the homogenate of the cells were remarkably reduced. These results strongly suggested that the inhibition of PGE2 production by vitamin E results from the inhibition of the activities of both phospholipase A2 and cyclooxygenase.
authors.affiliation: Department of Biochemistry, School of Dentistry, Hokkaido University, Sapporo, Japan.
authors.forename: W
authors.initials: W
authors.lastname: Sakamoto
authors.forename: K
authors.initials: K
authors.lastname: Fujie
authors.forename: J
authors.initials: J
authors.lastname: Nishihira
authors.forename: M
authors.initials: M
authors.lastname: Mino
authors.forename: I
authors.initials: I
authors.lastname: Morita
authors.forename: S
authors.initials: S
authors.lastname: Murota
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D015232:Dinoprostone / Q000096:biosynthesis*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D016861:Cyclooxygenase Inhibitors; D014810:Vitamin E; D016718:Arachidonic Acid; D000001:Calcimycin; D010741:Phospholipases A; D054467:Phospholipases A2; D015232:Dinoprostone; D013755:Tetradecanoylphorbol Acetate