Di-n-pentyl phthalate-induced inflammatory changes in the rat testis are accompanied by local production of a novel lymphocyte activating factor.
Phthalate esters (PAE) are plasticizers in polyvinyl chloride plastics used, for example, in package material for medical solutions. PAE exposure is associated with testicular damage that primarily affects Sertoli cells, and is concomitant with leukocyte infiltration into the testicular interstitium. We have demonstrated that the rat testis constitutively produces a lymphocyte activating factor (LAF) resembling interleukin-1 alpha, and originating from Sertoli cells. The testicular interleukin-1-like factor (tIL-1) has a relative molecular mass (Mr) of 17,000 (17 k) and an isoelectric point (pI) of 5.7. In the present study we have measured testicular LAF activity after exposure to di-n-pentyl phthalate (DPP) in 40-day-old rats. We found a large increase in LAF bioactivity which was evident already 3 h after a single oral dose of DPP. The increase was maximal 9-12 h after exposure, and had decreased toward the control level at 24 h. The increased activity was found to be at least partly due to the induction of a novel LAF with Mr 38,000 and pI 4.5. Morphological examination confirmed earlier results with an interstitial leukocyte infiltration 6 h after DPP exposure. The identity of the novel LAF and its functional relation to testicular inflammation remain to be established.
Granholm
T
T
Pediatric Endocrinology Unit, Karolinska Hospital, Stockholm, Sweden.
Creasy
D M
DM
Pöllänen
P
P
Söder
O
O
D000818:Animals; D001681:Biological Assay; D016207:Cytokines / Q000737:chemistry / Q000378:metabolism*; D007249:Inflammation / Q000503:physiopathology*; D007375:Interleukin-1 / Q000737:chemistry / Q000378:metabolism; D008213:Lymphocyte Activation; D008297:Male; D010795:Phthalic Acids / Q000494:pharmacology* / Q000633:toxicity; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000473:pathology / Q000503:physiopathology*; D013997:Time Factors
D016207:Cytokines; D007375:Interleukin-1; D010795:Phthalic Acids; C034171:di-n-pentyl phthalate