A technique for quantitative cytology of nasal secretions.
To quantitatively analyze cellular elements in mucopurulent nasal secretions from patients with chronic paranasal sinusitis, we studied five mucus-liquefying agents--dithiothreitol (DTT), ethylenediaminetetraacetic acid tetrasodium salt, propylene glycol, serratio peptidase and urea--with Hanks' balanced salt solution used as a control. Agents were compared for the number of cells, the mucus-liquefying effect and the staining characteristic of cells in cytocentrifuge-prepared smears. The results indicated that the treatment of nasal secretions with 10 mM DTT provides more clear and detailed cytological preparations for quantitative cytology of nasal secretions.
Lee
H S
HS
Department of Otorhinolaryngology, Mie University School of Medicine, Japan.
Majima
Y
Y
Sakakura
Y
Y
Kim
B W
BW
D002452:Cell Count; D002908:Chronic Disease; D003584:Cytological Techniques; D004229:Dithiothreitol; D004492:Edetic Acid; D006801:Humans; D009093:Mucus / Q000166:cytology*; D009297:Nasal Mucosa / Q000378:metabolism*; D010447:Peptide Hydrolases; D019946:Propylene Glycol; D011409:Propylene Glycols; D012852:Sinusitis / Q000503:physiopathology*; D014508:Urea
D011409:Propylene Glycols; D019946:Propylene Glycol; D014508:Urea; D004492:Edetic Acid; D010447:Peptide Hydrolases; C022640:serratiopeptidase; D004229:Dithiothreitol