Individual-specific patterns of human variable genomic regions detected by a DNA probe from the HIV-1 env gene.
It was postulated that similar genetic elements that are 'hot spots' for genetic variation might exist in both the HIV-1 and the human genome. To test this possibility a short repeated sequence from a region of variability in the HIV-1 glycoprotein (env) gene was amplified and used as a probe for blot hybridization with human genome DNA. Human genomic regions were hybridized and characterized by a set of polymorphic restriction DNA fragments. The pattern of the restriction fragments was individual specific. Thus a DNA probe from the HIV-1 env gene can serve as a genetic marker for hybridization with human genome regions and for the identification of individuals.
Rogaev
E I
EI
National Mental Research Centre, Academy of Medical Sciences of the USSR, Moscow.
Shlenskii
A B
AB
Spoode AYa
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D015342:DNA Probes; D004279:DNA, Viral / Q000737:chemistry; D015752:Genes, env; D014644:Genetic Variation; D016679:Genome, Viral; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D004279:DNA, Viral