DNA binding activity of recombinant SRY from normal males and XY females.
The protein encoded by the human testis determining gene, SRY, contains a high mobility group (HMG) box related to that present in the T cell-specific, DNA-binding protein TCF-1. Recombinant SRY protein was able to bind to the same core sequence AACAAAG recognized by TCF-1 in a sequence dependent manner. In five XY females point mutations were found in the region encoding the HMG box. In four cases DNA binding activity of mutant SRY protein was negligible; in the fifth case DNA binding was reduced. These results imply that the DNA binding activity of SRY is required for sex determination.
Harley
V R
VR
Human Molecular Genetics Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
Jackson
D I
DI
Hextall
P J
PJ
Hawkins
J R
JR
Berkovitz
G D
GD
Sockanathan
S
S
Lovell-Badge
R
R
Goodfellow
P N
PN
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors / Q000378:metabolism
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins; C497745:SRY protein, human; D051876:Sex-Determining Region Y Protein; C497746:Sry protein, mouse; D014157:Transcription Factors