title: The amino acid sequence of glutamate decarboxylase from Escherichia coli. Evolutionary relationship between mammalian and bacterial enzymes.
abstract: The amino acid sequence of glutamate decarboxylase from Escherichia coli was solved by a combination of automated Edman degradation of peptide fragments derived by proteolytic and chemical cleavage and sequencing of DNA. Correct alignment of three peptides, for which no peptide overlaps were available, was achieved by sequencing a 1.1-kbp fragment of DNA produced by a polymerase-chain reaction using primers corresponding to sequences known to be in amino-terminal and carboxy-terminal regions of the protein. Sequence similarity (24% identity) with mammalian glutamate decarboxylase was found to be limited to a 55-residue sequence around the lysine residue that binds the coenzyme. Stronger similarity (38% identity), again confined to the same region, is seen with bacterial pyridoxal-phosphate-dependent histidine decarboxylase.
authors.affiliation: Dipartimento di Scienze Biochimiche, A. Rossi Fanelli, Universita La Sapienza, Roma, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Maras
authors.forename: G
authors.initials: G
authors.lastname: Sweeney
authors.forename: D
authors.initials: D
authors.lastname: Barra
authors.forename: F
authors.initials: F
authors.lastname: Bossa
authors.forename: R A
authors.initials: RA
authors.lastname: John
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002415:Cats; D004269:DNA, Bacterial / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005968:Glutamate Decarboxylase / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004269:DNA, Bacterial; D005968:Glutamate Decarboxylase