title: [Biosynthesis of ecdysteroids by prothoracic glands in Pieris brassicae (Lepidoptera insect). Conversion in vitro of radiolabelled precursor of 3-dehydroecdysone].
abstract: Ecdysteroids secreted by prothoracic glands of Pieris brassicae were measured by enzyme immunoassay, after HPLC separation. Both ecdysone and 3-dehydroecdysone, as a major product, are produced. Two radiolabelled putative precursors were converted by Pieris glands in vitro: [3H] ketodiol was converted into ecdysone at a low rate; [3H]3-oxoketodiol was efficiently converted, mainly into 3-dehydroecdysone. These data make 3-oxoketodiol a good candidate for an intermediate in ecdysteroid biosynthetic pathway.
authors.affiliation: C.N.R.S.-U.R.A. 686, Ecole normale supérieure, Paris.
authors.forename: C
authors.initials: C
authors.lastname: Blais
authors.forename: R
authors.initials: R
authors.lastname: Lafont
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004441:Ecdysterone / Q000096:biosynthesis*; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D007447:Invertebrate Hormones / Q000096:biosynthesis*; D007553:Isotope Labeling; D007915:Lepidoptera / Q000033:anatomy & histology / Q000378:metabolism*; D011355:Prodrugs / Q000378:metabolism*
chemical_list: D007447:Invertebrate Hormones; D011355:Prodrugs; D004441:Ecdysterone
vernacular_title: Biosynthèse des ecdystéroïdes par les glandes prothoraciques de Pieris brassicae (insecte Lépidoptère). Conversion in vitro d'un précurseur radioactif de la 3-déhydroecdysone.