Mass spectrometry in the study of advanced glycation processes, responsible for long-term diabetes complications.
Mass spectrometry has been usefully employed in the study of the products arising from in vitro and in vivo glycation of proteins. In particular, daughter-ion spectroscopy has led to an easy detection of 2-(2-furoyl)4-(5)2(furanyl)1-H-imidazole in HCl-hydrolysed glycated albumen and polylysine and the method has allowed us to exclude the presence of the same molecule in in vivo glycated proteins. Parent-ion spectroscopy has been successfully employed in the identification of furoyl-containing compounds, which are possibly responsible for the results obtained with other analytical approaches.
Lapolla
A
A
Istituto de Medicina Interna, Università di Padova, Italy.
Fedele
D
D
Seraglia
R
R
Traldi
P
P
D000418:Albumins / Q000378:metabolism*; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D066298:In Vitro Techniques; D013058:Mass Spectrometry / Q000379:methods; D011107:Polylysine / Q000378:metabolism*; D011108:Polymers / Q000032:analysis; D051381:Rats
D000418:Albumins; D011108:Polymers; C011908:melanoidin polymers; D011107:Polylysine; D005947:Glucose