title: A quantitative immunoelectronmicroscopic study on soluble, membrane-associated and membrane-bound lysosomal enzymes in human intestinal epithelial cells.
abstract: We have used quantitative immunoelectronmicroscopy to compare the in situ localization of acid alpha-glucosidase, lysosomal acid phosphatase, beta-hexosaminidase and glucocerebrosidase in intestinal epithelial cells of the human duodenum. Differences between these four lysosomal enzymes were observed with respect to their presence at the apical cell surface. Transport to the apical membrane seems to be a more important intracellular route for lysosomal acid phosphatase and acid alpha-glucosidase than it is for beta-hexosaminidase. The membrane associated lysosomal enzyme glucocerebrosidase is not transported to the microvilli. The studies emphasize that lysosomal enzyme transport pathways are enzyme and cell type specific.
authors.affiliation: MGC-Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Willemsen
authors.forename: R
authors.initials: R
authors.lastname: Brünken
authors.forename: C W
authors.initials: CW
authors.lastname: Sorber
authors.forename: A T
authors.initials: AT
authors.lastname: Hoogeveen
authors.forename: H A
authors.initials: HA
authors.lastname: Wisselaar
authors.forename: J M
authors.initials: JM
authors.lastname: Van Dongen
authors.forename: A J
authors.initials: AJ
authors.lastname: Reuser
mesh_terms: D002462:Cell Membrane / Q000201:enzymology; D004386:Duodenum / Q000201:enzymology; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D016253:Microscopy, Immunoelectron