title: Primary structure and import pathway of the rotenone-insensitive NADH-ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae.
abstract: The gene encoding the yeast mitochondrial rotenone-insensitive internal NADH: ubiquinone-6 oxidoreductase has been sequenced. The DNA sequence indicates the presence of an open reading frame of 1539 bp predicted to encode a protein of 513 amino acid residues (57.2 kDa). The NADH dehydrogenase is synthesized as a precursor protein containing a signal sequence of 26 residues. In vitro import experiments show that the precursor NADH dehydrogenase is cleaved to the mature size by the matrix processing peptidase. Both cleavage and translocation across the mitochondrial membrane(s) are dependent on the membrane potential component of the proton-motive force. Comparison of the protein sequence of the yeast NADH dehydrogenase with the data bank indicates that the enzyme from yeast is homologous to the NADH dehydrogenase of Escherichia coli (22.2% identical residues). Both NADH dehydrogenases contain in the central part of the protein a sequence predicted to fold into a beta alpha beta structure involved in the binding of NADH or FAD(H2). Various aspects of the protein structure are discussed.
authors.affiliation: Department of Molecular Cell Biology, University of Amsterdam, The Netherlands.
authors.forename: S
authors.initials: S
authors.lastname: De Vries
authors.forename: R
authors.initials: R
authors.lastname: Van Witzenburg
authors.forename: L A
authors.initials: LA
authors.lastname: Grivell
authors.forename: C A
authors.initials: CA
authors.lastname: Marres
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001692:Biological Transport; D004271:DNA, Fungal / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D007425:Intracellular Membranes / Q000502:physiology; D008564:Membrane Potentials; D008928:Mitochondria / Q000201:enzymology* / Q000502:physiology; D008969:Molecular Sequence Data; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics* / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000235:genetics / Q000378:metabolism; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D012402:Rotenone / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D016415:Sequence Alignment; D014158:Transcription, Genetic
chemical_list: D004271:DNA, Fungal; D004792:Enzyme Precursors; D012402:Rotenone; D016660:NAD(P)H Dehydrogenase (Quinone); D009245:NADH Dehydrogenase