A monoclonal antibody that labels Purkinje cells in the rat cerebellum.
A monoclonal antibody (MAb-1D10), generated by immunizing Balb/c mice with homogenized bovine retinal tissue, labeled the Müller cells in the bovine retina and the Purkinje cells of the rat cerebellum. In the neonatal rat cerebellum, immunoreactivity for MAb-1D10 appeared first in the ventral regions of vermis (lobuli I-III and IX-X) on the 11th postnatal day (P11), while in the dorsal vermis and cerebellar hemisphere, the immunoreactive Purkinje cells were detected on P13. Western blotting analysis disclosed that MAb-1D10 recognized bovine retinal polypeptides with mol.wt. of 48 kDa. Transplantation of the rat cerebellar primordia on 16th embryonic day demonstrated large and round cells with a linear arrangement in the grafts surviving well in the host rat striatum after 50 days of the grafting. These large cells were immunohistochemically labeled by MAb-1D10. No other cells in both the graft and the host striatum were immunoreactive to the MAb.
Tokunaga
A
A
Third Department of Anatomy, School of Medicine, Okayama University, Japan.
Ono
K
K
Date
I
I
Arisawa
T
T
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D015153:Blotting, Western; D016380:Brain Tissue Transplantation; D002417:Cattle; D002531:Cerebellum / Q000033:anatomy & histology / Q000166:cytology* / Q000637:transplantation; D016332:Fetal Tissue Transplantation; D006168:Guinea Pigs; D007074:Immunoglobulin G; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008970:Molecular Weight; D011689:Purkinje Cells / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D007074:Immunoglobulin G