title: Oral immunization with bacterial polysaccharide and adjuvant enhances antigen-specific pulmonary secretory antibody response and resistance to pneumonia.
abstract: Nosocomial pneumonia, often due to Pseudomonas aeruginosa, occurs frequently after haemorrhage and trauma, and contributes to the increased incidence of morbidity and mortality in this clinical setting. In order to determine if enhancement of bacterial antigen-specific secretory IgA (sIgA) titres in the lungs can increase resistance to P. aeruginosa pneumonia following haemorrhage, we investigated oral immunization strategies, using bacterial polysaccharides (levan, from Aerobacter levanicum, and P. aeruginosa polysaccharide type I) and adjuvant (cholera toxin and the B-subunit of cholera toxin), capable of increasing bacterial polysaccharide-specific pulmonary secretory antibody titres. Oral co-administration of 1000 micrograms levan and 10 micrograms cholera toxin resulted in increased titres of levan-specific sIgA in lung lavages and increased numbers of levan-specific pulmonary plasma cells, but no changes in serum anti-levan titres. Similarly, oral co-administration of 1000 micrograms P. aeruginosa polysaccharide and 10 micrograms cholera toxin produced increased anti-P. aeruginosa polysaccharide titres in lung lavages. Significant decreases in anti-levan pulmonary sIgA titres and in numbers of levan-specific pulmonary plasma cells were found when oral immunization with levan and cholera toxin was performed 4 days following haemorrhage, but not if the mice were immunized 8 h after blood loss. Although haemorrhage markedly increased the susceptibility of mice to P. aeruginosa pneumonia, significant protection from mortality could be achieved through oral immunization with 1000 micrograms P. aeruginosa polysaccharide and 10 micrograms cholera toxin 8 h after haemorrhage. These results demonstrate that haemorrhage induces marked alterations in bacterial antigen-specific pulmonary B-cell responses, which contribute to the increased susceptibility to infection in this setting.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Medicine, UCLA Medical Center 90024.
authors.forename: E
authors.initials: E
authors.lastname: Abraham
authors.forename: A
authors.initials: A
authors.lastname: Robinson
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000918:Antibody Specificity / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology; D002772:Cholera Toxin / Q000276:immunology*; D006470:Hemorrhage / Q000150:complications / Q000276:immunology; D007113:Immunity, Innate / Q000276:immunology; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis*; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011014:Pneumonia / Q000150:complications / Q000276:immunology* / Q000401:mortality; D011135:Polysaccharides, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D011552:Pseudomonas Infections / Q000150:complications / Q000276:immunology / Q000401:mortality; D015996:Survival Rate; D014611:Vaccination
chemical_list: D000942:Antigens, Bacterial; D007071:Immunoglobulin A, Secretory; D011135:Polysaccharides, Bacterial; D002772:Cholera Toxin