Immunity to melanoma in mice immunized with transfected allogeneic mouse fibroblasts expressing melanoma-associated antigens.
Transfection of genomic DNA from B16 mouse melanoma into LM(TK-) fibroblasts led to the generation of several clones of transfected cells that strongly expressed B16 melanoma-associated antigens (MAA). The transfected cells retained their H-2k markers and served as allogenic cells with expressive MAA in C57BL/6 mice, syngeneic with the melanoma. The cells were capable of eliciting primary anti-B16 immune responses in vitro in spleen cells from C57BL/6 mice. Immunization of C57BL/6 mice with the transfected cells led to the generation of anti-B16 cytotoxic activity in spleen cells, and C57BL/6 mice immunized with the MAA-positive transfected cells were partially resistant to a lethal challenge with B16 melanoma cells. Under similar conditions, B16 cells were nonimmunogenic. Therefore, transfected allogeneic LM(TK-) fibroblast cells expressing MAA served as more potent anti-melanoma immunogens than the parental B16 tumor cells themselves.
Kim
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Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago 60680.
Slomski
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Cohen
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D000818:Animals; D000951:Antigens, Neoplasm; D004247:DNA / Q000235:genetics; D004912:Erythrocytes / Q000276:immunology; D005347:Fibroblasts / Q000276:immunology / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D006183:H-2 Antigens / Q000502:physiology; D064532:Histocompatibility Antigen H-2D; D007111:Immunity, Cellular / Q000276:immunology; D007114:Immunization; D008285:Major Histocompatibility Complex / Q000502:physiology; D008546:Melanoma, Experimental / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D058950:Melanoma-Specific Antigens; D051379:Mice; D008810:Mice, Inbred C57BL; D009363:Neoplasm Proteins / Q000235:genetics* / Q000276:immunology; D012397:Rosette Formation; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014162:Transfection; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D004247:DNA