Sequential expression of JUN B, JUN D and FOS B proteins in rat spinal neurons: cascade of transcriptional operations during nociception.
Expression of the immediate-early gene encoded proteins JUN B, JUN D and FOS B was investigated by immunocytochemistry in rat L5 spinal cord up to 24 h following stimulation of hind limb somatosensory nociceptors by noxious heat or injection of formalin. In both experimental protocols, JUN B, which did not show basal expression, reached maximum expression after 2 h and thereafter slowly decreased. In contrast, the expression of JUN D, which was present before stimulation in many spinal neurons, was increased after 4 h, reached its maximum after 8 h and thereafter remained elevated. FOS B which was absent under basal conditions reached its maximum between 4 h and 8 h and thereafter declined but was still present after 24 h. All immunoreactivities were restricted to the ipsilateral dorsal horn except JUN D which was also induced in the contralateral side after 8 h. The results are discussed in respect to their meaning for transcriptional operations of JUN and FOS proteins.
Herdegen
T
T
II. Physiologisches Institut der Universität Heidelberg, F.R.G.
Tölle
T R
TR
Bravo
R
R
Zieglgänsberger
W
W
Zimmermann
M
M
D000818:Animals; D005557:Formaldehyde / Q000494:pharmacology; D005786:Gene Expression Regulation; D006358:Hot Temperature; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000378:metabolism*; D009619:Nociceptors / Q000378:metabolism; D010146:Pain / Q000378:metabolism* / Q000503:physiopathology; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D014158:Transcription, Genetic
D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D005557:Formaldehyde