A novel approach to the rapid isolation and nucleotide sequencing of genomic clones.
In this study, a genomic library subdivided into fractions was rapidly screened by a Southern detection technique. Deletion libraries were obtained from recovered genomic clones by single random cuts with nuclease S1. These deletion libraries proved useful for localizing genes in the inserts and yielded, after size fractionation, nested deletions suitable for nucleotide sequencing. An heterologous vector (pDB21) carried the insert used as probe for all hybridizations involved in the process of genomic clones isolation and characterization.
Boivin
R
R
Department of Biochemistry, Faculty of Sciences and Engineering, Laval University, Québec, Canada.
Bellemare
G
G
D001483:Base Sequence; D015139:Blotting, Southern; D001937:Brassica / Q000235:genetics; D002872:Chromosome Deletion; D004247:DNA / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D015698:Genomic Library; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics; D015183:Restriction Mapping
D004247:DNA