Decrease of proliferation rate and induction of differentiation by a MYCN antisense DNA oligomer in a human neuroblastoma cell line.
The effects of an antisense oligodeoxynucleotide to codons 2-7 of the oncogene MYCN on the human neuroblastoma cell line LAN-5 were studied. Treated cells showed a decreased MYCN protein expression and synthesis by immunoperoxidase staining and immunoprecipitation. At the same time, the replication rate was inhibited, and the phenotype was modified toward a more differentiated type. Our data suggest the involvement of oncogene MYCN in both proliferative and differentiative processes.
Negroni
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A
Division of Physics and Biochemical Sciences, Ente per le nuove technologie per l'energia e l'ambiente (ENEA), Rome, Italy.
Scarpa
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S
Romeo
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A
Ferrari
S
S
Modesti
A
A
Raschellà
G
G
D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002455:Cell Division / Q000187:drug effects* / Q000235:genetics; D002461:Cell Line, Transformed; D004261:DNA Replication / Q000187:drug effects; D016373:DNA, Antisense / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D016259:Genes, myc / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D016900:Neurofilament Proteins / Q000032:analysis; D014746:Vimentin / Q000032:analysis
D016373:DNA, Antisense; D016900:Neurofilament Proteins; D014746:Vimentin