title: Expression of bacterial mercuric ion reductase in Saccharomyces cerevisiae.
abstract: The gene merA coding for bacterial mercuric ion reductase was cloned under the control of the yeast promoter for alcohol dehydrogenase I in the yeast-Escherichia coli shuttle plasmid pADH040-2 and transformed into Saccharomyces cerevisiae AH22. The resulting transformant harbored stable copies of the merA-containing hybrid plasmid, displayed a fivefold increase in the MIC of mercuric chloride, and synthesized mercuric ion reductase activity.
authors.affiliation: Institut für Pflanzenphysiologie und Mikrobiologie, Freie Universität Berlin, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Rensing
authors.forename: U
authors.initials: U
authors.lastname: Kües
authors.forename: U
authors.initials: U
authors.lastname: Stahl
authors.forename: D H
authors.initials: DH
authors.lastname: Nies
authors.forename: B
authors.initials: B
authors.lastname: Friedrich
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000235:genetics; D008627:Mercuric Chloride / Q000494:pharmacology*; D010088:Oxidoreductases / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics
chemical_list: D008627:Mercuric Chloride; D010088:Oxidoreductases; D000426:Alcohol Dehydrogenase; C021551:mercuric reductase