IgA testing for diagnosis of retroviral infections in the Caribbean.
In Guadeloupe, a Caribbean island with a high prevalence of HTLV-I infected subjects, the percentage of false positive results for HIV IgG is high, requiring additional time and expense for confirmatory tests. This article describes a simple way of overcoming this problem. First, all IgG and IgG immune complexes are removed by protein G treatment, and then IgA enzyme-linked immunosorbent assay (ELISA) and the Western blot test are performed with minor modification of commercially available kits.
Ridel
P R
PR
Institut Pasteur de la Guadeloupe, Pointe à Pitre, France.
Artus
J C
JC
Capron
A
A
D015153:Blotting, Western / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005189:False Positive Reactions; D015483:HIV Antibodies / Q000032:analysis*; D015658:HIV Infections / Q000175:diagnosis*; D015490:HTLV-I Infections / Q000175:diagnosis*; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis; D009419:Nerve Tissue Proteins; D011933:Reagent Kits, Diagnostic; D014900:West Indies
C029152:G-substrate; D015483:HIV Antibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D009419:Nerve Tissue Proteins; D011933:Reagent Kits, Diagnostic