[An antiserum directed against phenobarbital with an organometallic tracer: production and characterization].
In order to develop a new immunoassay procedure based on the use of organometallic labels, antisera are raising against sedative-hypnotic drug: phenobarbital. Immunization was performed in rabbits with parasuccinylamidophenobarbital coupled to bovine-serum-albumin. The evaluation of the specificity and the affinity constants of antisera are studied against different hypnotic or antiepileptic drugs and the metallotracer (phenobarbital labelled with an organometallic fragment derived from cymantrene). The most important result of this study indicates that the organometallic moiety of the metallotracer did not disturb neither the inhibition (% inhibition = 110) nor the affinity constant (Ka = 2.10(8) L/M) of the phenobarbital.
Mariet
F
F
Unité d'immunoanalyse, Faculté de Pharmacie, Dijon, France.
Brossier
P
P
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation; D009942:Organometallic Compounds / Q000276:immunology*; D010634:Phenobarbital / Q000276:immunology*; D011817:Rabbits / Q000276:immunology
D000906:Antibodies; D009942:Organometallic Compounds; D010634:Phenobarbital
Etude d'un antisérum dirigé contre le phénobarbital vis-à-vis d'un traceur organométallique: production et caractérisation.