2-Deoxy-2-fluoro-D-galactose protein N-glycosylation.
2-Deoxy-2-fluoro-D-galactose (dGalF), added to the medium of primary cultured rat hepatocytes, inhibited N-glycosylation of membrane (gp 120) and secretory glycoproteins (alpha 1-macroglobulin) in a concentration-dependent manner. Complete inhibition of N-glycosylation was achieved at concentrations of 1 mM and above. At identical concentrations, 2-deoxy-2-fluoro-D-glucose (dGlcF) caused only incomplete inhibition of N-glycosylation. dGalF reduced incorporation of D-[2,6-3H]mannose into lipid-linked oligosaccharides indicating interference with their assembly in the dolichol cycle.
Loch
N
N
Institut für Molekularbiologie und Biochemie Freien Universität Berlin, Germany.
Geilen
C C
CC
Spörndle
I
I
Oberdorfer
F
F
Keppler
D
D
Tauber
R
R
Reutter
W
W
D000818:Animals; D002478:Cells, Cultured; D003847:Deoxyglucose / Q000031:analogs & derivatives / Q000494:pharmacology; D004288:Dolichol Phosphates / Q000378:metabolism; D019788:Fluorodeoxyglucose F18; D005643:Fucose / Q000031:analogs & derivatives* / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism; D051381:Rats; D014415:Tunicamycin / Q000494:pharmacology
D004288:Dolichol Phosphates; D006023:Glycoproteins; D008565:Membrane Proteins; D009844:Oligosaccharides; D019788:Fluorodeoxyglucose F18; D014415:Tunicamycin; D005643:Fucose; D003847:Deoxyglucose; C049289:2-deoxy-2-fluorogalactose; D008358:Mannose