title: Immunoaffinity purification and cDNA cloning of human platelet prostaglandin endoperoxide synthase (cyclooxygenase).
abstract: The cDNA for prostaglandin endoperoxide synthase (cyclooxygenase) was cloned from human platelets by the polymerase chain reaction amplification method, and the primary structure of the enzyme was deduced from the nucleotide sequence. The enzyme was composed of 599 amino acids including 23-amino acid signal sequence, and the calculated molecular weight of the mature protein was 65,995. The enzyme was immunoaffinity-purified from human platelets. The N-terminal amino acid sequence determined by Edman degradation was Ala-Asp-Pro-Gly-Ala-Pro-Thr-Pro-, and the result confirmed the primary structure of the enzyme, which was deduced from the cDNA sequence.
authors.affiliation: Department of Biochemistry, Tokushima University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: N
authors.initials: N
authors.lastname: Ueda
authors.forename: T
authors.initials: T
authors.lastname: Yoshimoto
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
authors.forename: C
authors.initials: C
authors.lastname: Yokoyama
authors.forename: A
authors.initials: A
authors.lastname: Miyata
authors.forename: T
authors.initials: T
authors.lastname: Tanabe
authors.forename: I
authors.initials: I
authors.lastname: Fuse
authors.forename: A
authors.initials: A
authors.lastname: Hattori
authors.forename: A
authors.initials: A
authors.lastname: Shibata
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D001241:Aspirin / Q000097:blood; D001483:Base Sequence; D001792:Blood Platelets / Q000201:enzymology*; D002846:Chromatography, Affinity; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000097:blood / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D011451:Prostaglandin-Endoperoxide Synthases / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA; D011451:Prostaglandin-Endoperoxide Synthases; D001241:Aspirin