[Kinetic characteristics of monoamine oxidase in brain regions of animals with varying degrees of sensitivity to 1-methyl, 4-phenyl, 1,2,3,6- tetrahydropyridine].
Kinetic parameters of monoamine oxidase (MAO) were studied in synaptosomal fraction P2 of brain compartments obtained from mice, rats, rabbits, cats, dogs, guinea pigs, macaque rhesus, green and javanese macaques using I-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) as a substrate. Rates of MAO-catalyzed reactions were also studied in animals sensitive to the dopaminergic neurotoxin. At low concentrations of MPTR (about 1-5 mM) the rate of MAO reaction corresponded to the index Vmax/Km. Among the animals studied the highest values of the index were found in guinea pigs and macaque rhesus, the lowest values -- in rabbits. Value of the Vmax/Km index correlated with neurosensitivity to MPTP in neuromelanin-positive and neuromelanin-negative animal groups. The biochemical index for estimation of MAO-catalyzed reactions rates may be used in evaluation of brain compartments neurosensitivity to the dopaminergic neurotoxin and its derivatives.
Ishkov
A G
AG
Ermakov
N V
NV
Mikerov
S V
SV
Miroshnichenko
E V
EV
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D008995:Monoamine Oxidase / Q000378:metabolism*; D011954:Receptors, Dopamine / Q000378:metabolism; D013045:Species Specificity; D013574:Synaptosomes / Q000201:enzymology
D011954:Receptors, Dopamine; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D008995:Monoamine Oxidase
Kineticheskie kharkteristiki monoaminoksidazy v otdelakh mozga zhivotnykh s raznoĭ stepen'iu chuvstvitel'nosti k 1-metil, 4-fenil, 1,2,3,6-tetragidropiridinu.