title: Chemotactic cytokine gene expression and production induced in human monocytes by membrane proteoglycans from Klebsiella pneumoniae.
abstract: The present study was designed to investigate the effect of membrane proteoglycans (MPG) from Klebsiella pneumoniae on production of the chemotactic cytokine, IL-8, and monocyte chemotactic protein (MCP) by human peripheral blood monocytes. Exposure of human peripheral blood monocytes to MPG in vitro induced high levels of mRNA transcripts for IL-8 and MCP, as assessed by Northern blot analysis. Cytokine gene expression was associated with the production of chemotactic activity in the supernatants. The levels of IL-8 and MCP expression induced by MPG were comparable with those elicited by LPS. Induction of chemotactic cytokines in mononuclear phagocytes may play a role in the immunomodulatory activity of MPG.
authors.affiliation: Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy.
authors.forename: W
authors.initials: W
authors.lastname: Luini
authors.forename: M
authors.initials: M
authors.lastname: De Rossi
authors.forename: L
authors.initials: L
authors.lastname: Licciardello
authors.forename: F
authors.initials: F
authors.lastname: Colotta
authors.forename: A
authors.initials: A
authors.lastname: Mantovani
mesh_terms: D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D011509:Proteoglycans / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D018932:Chemokine CCL2; D002630:Chemotactic Factors; D016209:Interleukin-8; D008070:Lipopolysaccharides; D011509:Proteoglycans; D012333:RNA, Messenger