Role of spectrin in Amoeba proteus, as studied by microinjection of anti-spectrin monoclonal antibodies.
Spectrin is a major protein accounting for about 5% of whole-cell proteins in Amoeba proteus, and the precipitation of spectrin by intracellular injection of purified anti-spectrin monoclonal antibodies has a profound effect on cell morphology, motility, and movement-related cell activities in amoebae. Thus, amoebae injected with anti-spectrin antibodies show drastic changes in their shape and movement, suggesting that amoeba spectrin plays an important structural role, unlike nonerythroid spectrins in other cells. However, precipitation of spectrin does not affect the distribution of F-actin in amoebae.
Choi
E Y
EY
Department of Zoology, University of Tennessee, Knoxville 37996.
Jeon
K W
KW
D000199:Actins / Q000032:analysis; D000656:Amoeba / Q000737:chemistry* / Q000166:cytology / Q000502:physiology; D000818:Animals; D000911:Antibodies, Monoclonal; D002465:Cell Movement; D008845:Microinjections; D008856:Microscopy, Fluorescence; D010587:Phagocytosis; D013049:Spectrin / Q000032:analysis / Q000276:immunology / Q000502:physiology*
D000199:Actins; D000911:Antibodies, Monoclonal; D013049:Spectrin