Wheat and barley allergens associated with baker's asthma. Glycosylated subunits of the alpha-amylase-inhibitor family have enhanced IgE-binding capacity.
A 16 kDa protein, designated CM16*, which strongly binds IgE from baker's-asthma patients has been identified as a glycosylated form of the previously reported WTAI-CM16, which is a subunit of the wheat tetrameric alpha-amylase inhibitor. A glycosylated form (CMb*) of BTAI-CMb, the equivalent inhibitor subunit from barley, has been also found to have significantly enhanced IgE-binding capacity. In all, 14 purified members of the alpha-amylase/trypsin-inhibitor family showed very different IgE-binding capacities when tested by a dot-blot assay. The glycosylated components CM16*, CMb* and the previously described non-glycosylated 14.5 kDa allergen from barley (renamed BMAI-1) were found to be the strongest allergens.
Sanchez-Monge
R
R
Departmento de Bioquímica, E.T.S. Ingenieros Agrónomos, Ciudad Universitaria, Madrid, Spain.
Gomez
L
L
Barber
D
D
Lopez-Otin
C
C
Armentia
A
A
Salcedo
G
G
D000485:Allergens / Q000276:immunology*; D000595:Amino Acid Sequence; D001249:Asthma / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D001467:Hordeum / Q000276:immunology*; D007073:Immunoglobulin E / Q000378:metabolism*; D008969:Molecular Sequence Data; D009784:Occupational Diseases / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology; D014908:Triticum / Q000276:immunology*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
D000485:Allergens; D010940:Plant Proteins; D007073:Immunoglobulin E; D000516:alpha-Amylases