Control of DNA synthesis genes in fission yeast by the cell-cycle gene cdc10+.
In the budding yeast Saccharomyces cerevisiae, cell-cycle control over DNA synthesis occurs partly through the coordinate expression in late G1 phase of many, if not all, of the genes required for DNA synthesis. A cis-acting hexamer element ACGCGT (an MluI restriction site) is responsible for coordinating transcriptional regulation of these genes at the G1/S phase boundary and we have identified a binding activity, DSC1, that recognizes these sequences in a cell-cycle-dependent manner. In the distantly related fission yeast Schizosaccharomyces pombe, only one of the known DNA synthesis genes, cdc22+, which encodes a subunit of ribonucleotide reductase, is periodically expressed in late G1 (ref. 6). The promoter region of cdc22+ has two MluI sites and five related sequences, suggesting that similar controls over DNA synthesis genes could occur in fission yeast. We report here a binding activity in fission yeast that is very similar to DSC1 in budding yeast. We also show that the fission yeast cdc10+ gene product, which is required for Start and entry into S phase, is a component of this binding activity.
Lowndes
N F
NF
Laboratory of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, UK.
McInerny
C J
CJ
Johnson
A L
AL
Fantes
P A
PA
Johnston
L H
LH
D000483:Alleles; D001483:Base Sequence; D002453:Cell Cycle; D004261:DNA Replication / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D013696:Temperature
D004271:DNA, Fungal; D005656:Fungal Proteins; D009838:Oligodeoxyribonucleotides