title: [Studies of the expression of human interleukin-2 receptor cDNA in mammalian cells].
abstract: Human interleukin-2 receptor cDNA was transfected into mouse fibroblast cells (L929) by using DNA-calcium phosphate coprecipitation method. Results from RNA dot-blot hybri dization, FITC-IL-2 fluorescent staining assay and anti-Tac specific rossette test demonstrated that the product of human interleukin-2 receptor cDNA in L929 cells is able to bind IL-2 and anti-Tac antibody. Moreover, the abnormal expression of IL-2R gene in T-cell leukemia cell lines such as Jukat cells and Molt-4 cells was analyzed and discussed.
authors.affiliation: Institute of Genetics, Fudan University, Shanghai.
authors.forename: Q M
authors.initials: QM
authors.lastname: Zou
authors.forename: W C
authors.initials: WC
authors.lastname: Cai
authors.forename: W H
authors.initials: WH
authors.lastname: Shen
authors.forename: X H
authors.initials: XH
authors.lastname: Zhu
authors.forename: C B
authors.initials: CB
authors.lastname: Li
authors.forename: S Y
authors.initials: SY
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006801:Humans; D051379:Mice; D009693:Nucleic Acid Hybridization; D015282:Octreotide; D015375:Receptors, Interleukin-2 / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D015375:Receptors, Interleukin-2; D004247:DNA; D015282:Octreotide