[Enzymatic synthesis of acyl peptides containing p-nitroanilides of basic amino acids].
A method is suggested for synthesis of acylpeptides, containing arginine or lysine p-nitroanilides at the C-terminus, via the acyl transfer reaction catalyzed by the Bacillus subtilis serine proteinase. Acyl-di- and acyltripeptide ethers with L- and D-amino acids were used as the carboxyl component taken in a twofold excess. When the concentration of dimethylformamide increases, the hydrolysis of the initial ether and the reaction product diminishes. Because of the enzyme inactivation by dimethylformamide the latter's optimal concentration is 70-80%.
Voiushina
T L
TL
Terent'eva
E Iu
EIu
Pozdnev
V F
VF
Gaĭda
A V
AV
Gololobov
M Iu
MIu
Liublinskaia
L A
LA
Stepanov
V M
VM
D000215:Acylation; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry*; D001412:Bacillus subtilis / Q000201:enzymology; D002384:Catalysis; D004126:Dimethylformamide / Q000737:chemistry; D007700:Kinetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D012697:Serine Endopeptidases / Q000378:metabolism
D000596:Amino Acids; D010455:Peptides; D004126:Dimethylformamide; D012697:Serine Endopeptidases
Fermentativnyĭ sintez atsilpeptidov, soderzhashchikh n-nitroanilidy osnovnykh aminokislot.