Identification of human ventricular myosin heavy chain fragments with monoclonal antibody 2F4 in human sera after myocardial necrosis.
An ELISA assay with monoclonal antibody (Mab 2F4) raised against human ventricular myosin heavy chains was developed and used to investigate human sera after myocardial infarction. The monoclonal antibody 2F4 was selected for its high affinity to soluble fragments of myosin heavy chains (subfragment-1) and for its appropriate tissue specificity. By including Mab 2F4 in a simple and rapid dot immunobinding assay sera from patients with acute chest pain and of persons without a history of heart disease were tested. Myosin was detected only in the sera of the patients with myocardial necrosis, confirmed by electrocardiographic data. Negative reactions in all control cases were found. The serum myosin fragments reactive with Mab 2F4 were characterized by immunoblot experiments and protein bands in the region about 43 kDa were found. It was concluded that the myocardial infarction can be demonstrated by detection of cardiac myosin heavy chain fragments in the patients' sera.
Simeonova
P P
PP
Department of Molecular Immunology, Bulgarian Academy of Sciences, Sofia.
Kehayov
I R
IR
Kyurkchiev
S D
SD
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D004586:Electrophoresis / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D006352:Heart Ventricles; D006801:Humans; D006825:Hybridomas; D007114:Immunization; D015151:Immunoblotting; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000175:diagnosis; D009206:Myocardium / Q000473:pathology*; D009218:Myosins / Q000032:analysis* / Q000276:immunology; D009336:Necrosis; D010446:Peptide Fragments / Q000032:analysis* / Q000276:immunology; D012967:Sodium Dodecyl Sulfate; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; D012967:Sodium Dodecyl Sulfate; D009218:Myosins