Deletion of pgi alters tryptophan biosynthesis in a genetically engineered strain of Escherichia coli.
Deletion of the structural gene for phosphoglucose isomerase (pgi) of Escherichia coli dramatically alters the path of glucose catabolism by diverting carbon into the hexose monophosphate shunt. The effect of this genetic alteration on the conversion of glucose to tryptophan by strains optimized for the biosynthesis of this amino acid was determined by using 13C-nuclear magnetic resonance spectroscopy in vivo. Pgi- strains converted glucose to tryptophan almost twice as efficiently as did their Pgi+ counterparts.
Mascarenhas
D
D
Western Research Center, Stauffer Chemical Company, Richmond, California 94804.
Ashworth
D J
DJ
Chen
C S
CS
D002872:Chromosome Deletion; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics*; D006019:Glycolysis; D009682:Magnetic Resonance Spectroscopy; D010427:Pentose Phosphate Pathway; D015202:Protein Engineering; D014364:Tryptophan / Q000096:biosynthesis* / Q000235:genetics
D014364:Tryptophan; D005956:Glucose-6-Phosphate Isomerase