Dodecyl maltoside detergent improves resolution of hepatic membrane proteins in two-dimensional gels.
This report describes the incorporation of an alkyl maltoside detergent in two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) sample lysis buffer in order to improve resolution of protein patterns separated by nonequilibrium pH gradient electrophoresis. Membrane-associated proteins with alkaline isoelectric points form horizontal streaks on two-dimensional electrophoretograms when solubilized with conventional nonionic detergent. Dodecyl maltoside enhances protein delipidation during solubilization and improves pattern resolution and protein mobility.
Witzmann
F
F
Molecular Anatomy Laboratory, Indiana University-Purdue University, Indianapolis, Columbus 47203.
Jarnot
B
B
Parker
D
D
D000818:Animals; D003902:Detergents; D015180:Electrophoresis, Gel, Two-Dimensional; D005960:Glucosides; D008565:Membrane Proteins / Q000032:analysis*; D008930:Mitochondria, Liver / Q000737:chemistry*; D051381:Rats
D003902:Detergents; D005960:Glucosides; D008565:Membrane Proteins; C040358:dodecyl maltoside