15NH4+ excretion test: a new method for detection of Helicobacter pylori infection.
A noninvasive test for the detection of Helicobacter pylori infection that uses [15N]urea as a tracer has been established. The principle the test is based on is the strong urease activity of H. pylori. After oral ingestion, [15N]urea is broken down into ammonia and carbon dioxide by H. pylori urease in the stomach. The ammonia is absorbed into the blood and excreted in the urine. The amount of [15N]urea, reflecting the magnitude of H. pylori infection, is evaluated by measuring the abundance and excretion rate of 15N in ammonia in the urine. Thirty-six patients were examined in our study. The 15N excretion rates in urine ammonia of patients who were H. pylori positive were significantly higher than those of H. pylori-negative patients (P less than 0.05). Twenty-three patients were H. pylori positive by Gram stain and culture. The sensitivity of the 15NH4 excretion test compared with these techniques was 96%, and no false positives were obtained. The 15NH4+ excretion rates of 13 H. pylori-negative subjects were all in the normal range (less than 0.3%). This method is a simple, precise, highly sensitive, noninvasive, nonradioactive test. It could be used for diagnosis as well as for the followup of patients receiving H. pylori eradication therapy, especially children and pregnant women. It could also be used in epidemiological investigation of H. pylori infection in a general population.
Wu
J C
JC
Department of Nuclear and Digestive Medicine, Xin Hua Hospital, Shanghai, People's Republic of China.
Liu
G L
GL
Zhang
Z H
ZH
Mou
Y L
YL
Chen
Q A
QA
Wu
J C
JC
Yang
S L
SL
D000328:Adult; D000641:Ammonia / Q000652:urine*; D001431:Bacteriological Techniques; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008297:Male; D008657:Metabolic Clearance Rate; D008875:Middle Aged; D009587:Nitrogen Isotopes; D011897:Random Allocation; D014508:Urea / Q000652:urine; D014510:Urease
D009587:Nitrogen Isotopes; D000641:Ammonia; D014508:Urea; D014510:Urease