The interaction of heparinized biomaterials with human serum, albumin, fibrinogen, antithrombin III, and platelets.
The influence of the method of heparin (HEP) immobilization on human serum albumin (HSA), fibrinogen (FG), and antithrombin III (AT-III) adsorption, platelet adhesion, and activation on the surface of polyvinylchloride, polyurethane Vitur, and a copolymer of styrene and divinylbenzene was measured. The negative correlation between the degree of irreversibility of plasma protein adsorption and the amount of adsorbed AT-III for HEP, immobilized onto the polymer surface passivated with HSA, FG, and plasma was found. The same negative correlation was observed between the amount of AT-III adsorbed on these systems and the number of adhered platelets. Schemes of the interaction of surface bound-HEP with AT-III, including the influence of an irreversibly adsorbed protein layer and adhered platelets, have been proposed. The essential role of AT-III in heparinized biomaterials/platelet interaction has been shown. A new method of combined immobilization of HEP and platelet adhesion inhibitor has been elaborated on.
Nemets
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EA
Biomaterials Center, Institute of Transplantology and Artificial Organs, Moscow, U.S.S.R.
Sevastianov
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D000990:Antithrombin III; D001672:Biocompatible Materials / Q000494:pharmacology*; D001792:Blood Platelets; D001798:Blood Proteins; D002404:Catheterization / Q000295:instrumentation; D005340:Fibrinogen; D006493:Heparin; D006801:Humans; D066298:In Vitro Techniques; D015539:Platelet Activation; D010973:Platelet Adhesiveness; D011140:Polyurethanes; D011143:Polyvinyl Chloride; D012709:Serum Albumin; D020058:Styrene; D013343:Styrenes; D014753:Vinyl Compounds
D001672:Biocompatible Materials; D001798:Blood Proteins; D011140:Polyurethanes; D012709:Serum Albumin; D013343:Styrenes; D014753:Vinyl Compounds; D020058:Styrene; D000990:Antithrombin III; D005340:Fibrinogen; D011143:Polyvinyl Chloride; D006493:Heparin; C004985:divinyl benzene