title: Cloning and characterization of two genes from Streptomyces lividans that confer inducible resistance to lincomycin and macrolide antibiotics.
abstract: Inducible resistance to lincomycin and macrolides in Streptomyces lividans TK21 results from expression of two linked genes: lrm, encoding a ribosomal RNA methyltransferase that confers high-level resistance to lincomycin with lower levels of resistance to macrolides, and mgt, encoding a glycosyl transferase that specifically inactivates macrolides using UDP-glucose as cofactor. The lrm and mgt genes have been cloned and sequenced. The deduced lrm product is a 26-kDa protein with much similarity to other ribosomal RNA methyltransferases, such as the carB, tlrA and ermE products, whereas the mgt product (predicted to be 42 kDa) resembles a eukaryotic glycosyl transferase. Macrolides that induce the lrm-mgt gene pair are substrates for inactivation by the mgt product, and the lrm product confers ribosomal resistance to such inducers.
authors.affiliation: Department of Biochemistry, University of Leicester, U.K.
authors.forename: G
authors.initials: G
authors.lastname: Jenkins
authors.forename: E
authors.initials: E
authors.lastname: Cundliffe
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D016695:Glycosyltransferases / Q000235:genetics; D008034:Lincomycin / Q000494:pharmacology*; D018942:Macrolides; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics*; D012359:tRNA Methyltransferases / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides; D008034:Lincomycin; D012359:tRNA Methyltransferases; D016695:Glycosyltransferases