SrfI, a new type-II restriction endonuclease that recognizes the octanucleotide sequence, [sequence: see text].
A new restriction endonuclease, SrfI has been isolated from an unidentified species of Streptomyces. SrfI recognizes the 8-bp palindrome, 5'-GCCCGGGC and cleaves double-stranded DNA after the third C in the sequence, producing blunt ends. SrfI is a rare-cutting enzyme and should therefore be useful for megabase mapping.
Simcox
T G
TG
Stratagene Cloning Systems, Inc., La Jolla, CA 92037.
Marsh
S J
SJ
Gross
E A
EA
Lernhardt
W
W
Davis
S
S
Simcox
M E
ME
D000256:Adenoviridae / Q000378:metabolism; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D009841:Oligonucleotides / Q000737:chemistry*; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity
D004279:DNA, Viral; D009841:Oligonucleotides; D015252:Deoxyribonucleases, Type II Site-Specific