title: Uptake of minocycline by Escherichia coli.
abstract: Uptake of tetracyclines into Escherichia coli was assessed with a strain carrying a tetA-lacZ translational fusion, in which expression of the enzyme is controlled by the pSC101 tetR repressor gene, by examining beta-galactosidase induction. The ability of tetracycline analogues to induce beta-galactosidase synthesis was correlated with their hydrophobicity, such that hydrophobic analogues were poor enzyme inducers. Treatment of E. coli with polymyxin B nonapeptide (PMBN) rendered cells more permeable to minocycline, but not to tetracycline.
authors.affiliation: Department of Microbial Biochemistry and Genetics, Lederle Laboratories, Pearl River, New York 10965.
authors.forename: I
authors.initials: I
authors.lastname: Chopra
authors.forename: K
authors.initials: K
authors.lastname: Hacker
mesh_terms: D004790:Enzyme Induction; D004926:Escherichia coli / Q000378:metabolism*; D008911:Minocycline / Q000493:pharmacokinetics*; D011112:Polymyxin B / Q000031:analogs & derivatives / Q000494:pharmacology; D013754:Tetracyclines / Q000493:pharmacokinetics; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D013754:Tetracyclines; C038934:polymyxin B nonapeptide; D001616:beta-Galactosidase; D008911:Minocycline; D011112:Polymyxin B