Crystallization of and preliminary X-ray data for the negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa.
The negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa has been purified from an over-expressing clone and crystalized. Crystals of diffraction quality were obtained from polyethylene glycol 4000 and ammonium sulphate. AmiC crystallizes in P4(2)2(1)2 (a = 104.4 A, c = 66.6 A) with one subunit in the asymmetric unit. Crystals diffract beyond 2.8 A.
Wilson
S A
SA
Department of Biochemistry and Molecular Biology, University College London, U.K.
Chayen
N E
NE
Hemmings
A M
AM
Drew
R E
RE
Pearl
L H
LH
D000581:Amidohydrolases / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D005798:Genes, Bacterial; D005809:Genes, Regulator; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D016366:Open Reading Frames; D009876:Operon; D033902:Periplasmic Binding Proteins; D011487:Protein Conformation; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*; D012097:Repressor Proteins / Q000737:chemistry; D015183:Restriction Mapping; D014961:X-Ray Diffraction / Q000379:methods
D001426:Bacterial Proteins; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D033902:Periplasmic Binding Proteins; D012097:Repressor Proteins; C070135:AmiC protein, Pseudomonas aeruginosa; D000581:Amidohydrolases