Expression of human glycophorin A in wild type and glycosylation-deficient Chinese hamster ovary cells. Role of N- and O-linked glycosylation in cell surface expression.
Glycophorin A, the most abundant sialoglycoprotein on human red blood cells, carries several medically important blood group antigens. To study the role of glycosylation in surface expression and antigenicity of this highly glycosylated protein (1 N-linked and 15 O-linked oligosaccharides), glycophorin A cDNA (M-allele) was expressed in Chinese hamster ovary (CHO) cells. Both wild type CHO cells and mutant CHO cells with well defined glycosylation defects were used. Glycophorin A was well expressed on the surface of transfected wild type CHO cells. On immunoblots, the CHO cells expressed monomer (approximately 38 kDa) and dimer forms of glycophorin A which co-migrated with human red blood cell glycophorin A. The transfected cells specifically expressed the M blood group antigen when tested with mouse monoclonal antibodies. Tunicamycin treatment of these CHO cells did not block surface expression of glycophorin A, indicating that, in the presence of normal O-linked glycosylation, the N-linked oligosaccharide is not required for surface expression. To study O-linked glycosylation, glycophorin A cDNA was transfected into the Lec 2, Lec 8, and ldlD glycosylation-deficient CHO cell lines. Glycophorin A with truncated O-linked oligosaccharides was well expressed on the surface of ldlD cells (cultured in the presence of N-acetylgalactosamine alone), Lec 2 cells, and Lec 8 cells with monomers of approximately 25 kDa, approximately 33 kDa, and approximately 25 kDa, respectively. In contrast, non-O-glycosylated glycophorin A (approximately 19-kDa monomers) was poorly expressed on the surface of ldlD cells cultured in the absence of both galactose and N-acetylgalactosamine. Thus, under these conditions, in the absence of O-linked glycosylation, the N-linked oligosaccharide itself is not able to support appropriate surface expression of glycophorin A in transfected CHO cells.
Remaley
A T
AT
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104.
Ugorski
M
M
Wu
N
N
Litzky
L
L
Burger
S R
SR
Moore
J S
JS
Fukuda
M
M
Spitalnik
S L
SL
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D016466:CHO Cells; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D005455:Fluorescent Antibody Technique; D006021:Glycophorins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D006031:Glycosylation; D006801:Humans; D009154:Mutation; D010957:Plasmids; D014162:Transfection; D014415:Tunicamycin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D006021:Glycophorins; D014415:Tunicamycin; D004247:DNA