Laser-induced fluorescence emission: I. The spectroscopic identification of fibrotic endocardium and myocardium.
Laser-induced fluorescence has been developed as a guidance system for laser angioplasty. Laser ablation has been used for resection of arrhythmogenic ventricular scar. We have investigated the use of laser-induced fluorescence for the detection of fibrotic and ischemic changes in endocardium and myocardium. Fluorescence emission spectra from human necropsy specimens were correlated with histologic examination. Normalized fluorescence intensity detected from both the endocardial and the myocardial surfaces of the fibrotic ventricular specimens was significantly higher than that of corresponding normal specimens at 440 to 475 nm. Fibrotic endocardium could be identified by a fluorescence emission intensity ratio less than 1.5 for wavelength ratio 375/450nm. Acutely infarcted endocardium was recognizable by a ratio of 1.5 to 2.0. The specificity and sensitivity of detection of scarred endocardium was 70 and 100%, respectively. Fibrotic myocardium was also consistently identified by fluorescence spectroscopy.


CONCLUSIONS
Fluorescence emission spectroscopy can differentiate normal and fibrotic endocardium and myocardium, in vitro. This technique may be useful for guidance during laser ablation of arrhythmogenic ventricular scar.
Perk
M
M
Department of Medicine, University of Alberta, Edmonton, Canada.
Flynn
G J
GJ
Smith
C
C
Bathgate
B
B
Tulip
J
J
Yue
W
W
Lucas
A
A
D002196:Capillaries / Q000473:pathology; D004489:Edema, Cardiac / Q000175:diagnosis / Q000473:pathology; D004699:Endocardium / Q000033:anatomy & histology / Q000473:pathology*; D004719:Endomyocardial Fibrosis / Q000175:diagnosis* / Q000473:pathology; D006470:Hemorrhage / Q000473:pathology; D006801:Humans; D007834:Lasers; D009203:Myocardial Infarction / Q000175:diagnosis* / Q000473:pathology; D009206:Myocardium / Q000473:pathology*; D009504:Neutrophils / Q000473:pathology; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods