title: FSH in testes of marmosets during development: immunocytochemical localization and de novo biosynthesis.
abstract: Immunocytochemical localization of FSH was carried out in various cell types of marmoset testes during development using antisera generated against intact as well as beta-subunit of human FSH. Significant differences in the intensity as well as distribution of FSH in various cell types were observed in neonatal, pubertal, and adult marmosets. Intensity of staining in Leydig cells was maximum at day 1 and in adults (1-3 years), whereas it was minimum at 3 months. In seminiferous tubules (Sertoli cells), FSH was present in trace amount until puberty and subsequently increased at maturity. Further studies demonstrate de novo biosynthesis of FSH-like moiety in vitro by testicular tissue, which was age dependent.
authors.affiliation: Institute for Research in Reproduction (ICMR), Parel, Bombay, India.
authors.forename: S V
authors.initials: SV
authors.lastname: Garde
authors.forename: A R
authors.initials: AR
authors.lastname: Sheth
authors.forename: S A
authors.initials: SA
authors.lastname: Kulkarni
mesh_terms: D000818:Animals; D002144:Callithrix / Q000254:growth & development*; D046508:Culture Techniques; D005640:Follicle Stimulating Hormone / Q000032:analysis* / Q000096:biosynthesis; D037201:Follicle Stimulating Hormone, beta Subunit; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007985:Leydig Cells / Q000737:chemistry*; D008297:Male; D012708:Sertoli Cells / Q000737:chemistry*; D013737:Testis / Q000737:chemistry* / Q000254:growth & development
chemical_list: D037201:Follicle Stimulating Hormone, beta Subunit; D005640:Follicle Stimulating Hormone