title: Uncoupling thermotolerance from the induction of heat shock proteins.
abstract: Exposure of cells to elevated temperatures causes a rapid increase in the synthesis of heat shock proteins (hsps) and induces thermotolerance, the increased ability of cells to survive exposure to lethal temperatures; however, the connection between hsp induction and the acquisition of thermotolerance is unclear. hsp induction in the yeast Saccharomyces cerevisiae is mediated by the activation of heat-shock transcription factor, and recently we have described a mutation, hsf1-m3, in heat-shock transcription factor that prevents the factor's activation. We now demonstrate that this mutation results in a general block in heat-shock induction but does not affect the acquisition of thermotolerance. Our results indicate that high-level induction of the major hsps is not required for cells to acquire thermotolerance.
authors.affiliation: University of California, San Diego, Department of Biology, La Jolla 92093.
authors.forename: B J
authors.initials: BJ
authors.lastname: Smith
authors.forename: M P
authors.initials: MP
authors.lastname: Yaffe
mesh_terms: D001483:Base Sequence; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D005800:Genes, Fungal; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009154:Mutation; D009838:Oligodeoxyribonucleotides; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D009838:Oligodeoxyribonucleotides; D001616:beta-Galactosidase; D019204:GTP-Binding Proteins