title: Repeated electroconvulsive shock increases tachykinin and cholecystokinin mRNA expression in ventral periaqueductal gray.
abstract: The effect of repeated electroconvulsive shock (five shocks during 10 days) on preprocholecystokinin and preprotachykinin-A messenger RNA expression was studied in the mesencephalic periaqueductal gray and adjacent areas of rat using in situ hybridization histochemistry with specific oligonucleotide probes. An increased number of preprocholecystokinin and preprotachykinin-A messenger RNA hybridization positive neurons (+30% and +47%, respectively) in the Edinger-Westphal nucleus was observed following repeated electroconvulsive shock. In addition, both preprocholecystokinin and preprotachykinin-A messenger RNA expression, measured as grain density over single neurons, was significantly increased (+37% and +45%, respectively). The results indicate that cholecystokinin- and substance P-containing neurons in the Edinger-Westphal nucleus are activated by repeated electroconvulsive shock, which may be related to the antidepressant and analgesic effects of electroconvulsive shock treatment.
authors.affiliation: Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: N
authors.initials: N
authors.lastname: Lindefors
authors.forename: K
authors.initials: K
authors.lastname: Brodin
authors.forename: C O
authors.initials: CO
authors.lastname: Stiller
authors.forename: H
authors.initials: H
authors.lastname: Persson
authors.forename: E
authors.initials: E
authors.lastname: Brodin
mesh_terms: D000818:Animals; D001483:Base Sequence; D002766:Cholecystokinin / Q000096:biosynthesis* / Q000235:genetics; D004597:Electroshock; D005786:Gene Expression Regulation; D008297:Male; D008636:Mesencephalon / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010487:Periaqueductal Gray / Q000378:metabolism*; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D015320:Tachykinins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011498:Protein Precursors; D012333:RNA, Messenger; D015320:Tachykinins; C044206:preprotachykinin; C042082:preprocholecystokinin; D002766:Cholecystokinin