Minisatellite binding protein Msbp-1 is a sequence-specific single-stranded DNA-binding protein.
Msbp-1 is a minisatellite-specific DNA-binding protein. Using synthetic binding substrates, we now show that Msbp-1 binds not to double-stranded DNA, but exclusively to single-stranded DNA. Binding is specific to the guanine-rich strand of the minisatellite duplex, interactions with the cytosine-rich strand being undetectable by southwestern analysis. Furthermore, the binding site required for successful DNA-protein interactions appears to be two or more minisatellite repeat units. We have also isolated, by whole-genome PCR and cloning, one Msbp-1 binding site from the human genome. Again, the binding strand of this molecule contains a repetitive G-rich structure equivalent to that of a small minisatellite. These observations are discussed with respect to other single-stranded DNA-binding proteins known to play a role in recombination processes.
Collick
A
A
Department of Genetics, University of Leicester, UK.
Dunn
M G
MG
Jeffreys
A J
AJ
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004276:DNA, Satellite / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D015894:Genome, Human; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004276:DNA, Satellite; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins