title: Quantity of protein deposited on hydrogel contact lenses and its relation to visible protein deposits.
abstract: The purposes of this study were to determine if the quantity of protein deposited (QPD) upon hydrogel lenses was affected by enzymatic cleaning and to test the potential relation between QPD and visible protein deposition (VPD) and change. Seventy-four contact lens patients classified as "heavy depositors" wore new lenses for an average of 80 (SD = 32) days. Cleaning and disinfection solutions varied. One lens was cleaned weekly by a papain enzymatic treatment. The distribution of QPD measurements was bimodal and was related to the FDA material for nonionic, low water content lenses (FDA Materials Group no. 1). The mean deposition was 45 micrograms/cm2 (N = 112) compared with that of ionic, high water content lenses (FDA Materials Group no. 4), which was 1010 micrograms/cm2 (N = 30). VPD distributions were the same for the FDA Group no. 1 and no. 4 lenses. Enzymatic treatment did not significantly reduce QPD; however, enzymatic treatment did reduce VPD. Thus QPD and VPD are independent phenomena and possible reasons for this are given.
authors.affiliation: Center for Corneal and Contact Lens Research, School of Optometry, University of Missouri, St. Louis.
authors.forename: R I
authors.initials: RI
authors.lastname: Myers
authors.forename: D W
authors.initials: DW
authors.lastname: Larsen
authors.forename: M
authors.initials: M
authors.lastname: Tsao
authors.forename: C
authors.initials: C
authors.lastname: Castellano
authors.forename: L D
authors.initials: LD
authors.lastname: Becherer
authors.forename: F
authors.initials: F
authors.lastname: Fontana
authors.forename: N R
authors.initials: NR
authors.lastname: Ghormley
authors.forename: G
authors.initials: G
authors.lastname: Meier
mesh_terms: D000328:Adult; D003261:Contact Lenses; D003902:Detergents; D004203:Disinfection / Q000379:methods; D004866:Equipment Contamination; D005136:Eye Proteins / Q000032:analysis* / Q000378:metabolism; D005260:Female; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D008297:Male; D010206:Papain; D011092:Polyethylene Glycols; D011485:Protein Binding
chemical_list: D003902:Detergents; D005136:Eye Proteins; C042434:tear proteins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; D010206:Papain