Alteration of second messenger systems after transient cerebral ischemia in gerbils: protective effect of pentobarbital and an autoradiographic analysis.
The postischemic alteration of second messenger systems in the gerbil brain was analyzed by receptor autoradiography using [3H]phorbol 12,13-dibutyrate (PDBu) and [3H]inositol 1,4,5-trisphosphate (IP3). The alteration of the [3H]PDBu binding in striatum and hippocampus was milder than that of the [3H]IP3 binding 5 h and 7 days after 10-min ischemia. The administration of pentobarbital prevented a decrease in the [3H]IP3 binding sites in all areas 5 h and 7 days after ischemia. These results suggest that the disruption of intracellular calcium homeostasis may play an important factor in ischemic brain damage.
Araki
T
T
Department of Neurology, Tohoku University School of Medicine, Sendai, Japan.
Kato
H
H
Kogure
K
K
D000818:Animals; D001345:Autoradiography; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D005849:Gerbillinae; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D015544:Inositol 1,4,5-Trisphosphate; D002546:Ischemic Attack, Transient / Q000378:metabolism* / Q000473:pathology; D008297:Male; D010424:Pentobarbital / Q000494:pharmacology*; D015240:Phorbol 12,13-Dibutyrate; D015290:Second Messenger Systems / Q000187:drug effects*
D015240:Phorbol 12,13-Dibutyrate; D015544:Inositol 1,4,5-Trisphosphate; D010424:Pentobarbital