Association of ubiquitin-activating enzyme with HeLa cell chromosomes during mitosis.
Ubiquitin-activating enzyme (E1) is the first enzyme in the pathway leading to formation of ubiquitin-protein conjugates. Antibodies raised against E1 were affinity purified and used for immunostaining HeLa cells. Condensed chromosomes in mitotic cells were found to be strongly immunoreactive. Chromosomes from metaphase-arrested HeLa cells were isolated and chromosome-associated proteins were analyzed by Western blotting. E1 was detected in fractions containing isolated chromosomes. These results suggest that E1 is associated with condensed chromosomes during mitosis.
Cook
J C
JC
Section of Metabolic Regulation, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
Chock
P B
PB
D015153:Blotting, Western; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D002877:Chromosomes, Human / Q000502:physiology* / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D007399:Interphase; D008025:Ligases / Q000378:metabolism*; D008938:Mitosis / Q000187:drug effects; D044764:Ubiquitin-Activating Enzymes; D044767:Ubiquitin-Protein Ligases; D014747:Vinblastine / Q000494:pharmacology
D014747:Vinblastine; D044767:Ubiquitin-Protein Ligases; D008025:Ligases; D044764:Ubiquitin-Activating Enzymes