Changes in cell envelope glycoproteins during germ-tube formation of Candida albicans.
Germ-tube formation by Candida albicans induced by N-acetylglucosamine resulted in the appearance of a 43 kD protein in a cell envelope fraction. The protein increased quantitatively in the cell envelope during the emergence of the germ-tube and the amount in the envelope fraction reflected the efficiency of the morphogenesis. The 43 kD protein was labelled by the lactoperoxidase catalysed iodination procedure confirming a surface location for the antigen. Concanavalin A binding to the 43 kD protein demonstrated that this protein contained carbohydrate. Tunicamycin inhibited both germ-tube formation in C. albicans and the appearance of the 43 kD protein in the cell envelope fraction. Instead the presence of tunicamycin resulted in the appearance of a new protein of 39 kD molecular weight in the cell envelope which did not bind concanavalin A. Endoglycosidase H digestion of the 43 kD protein produced a 39 kD protein. Peptide mapping of the 43 kD protein from germ-tube cells and the 39 kD protein from tunicamycin-treated cells indicated that these proteins are homologous.
Broom
M F
MF
Experimental Oral Biology Unit, School of Dentistry, University of Otago, Dunedin, New Zealand.
Shepherd
M G
MG
Sullivan
P A
PA
D001345:Autoradiography; D002176:Candida albicans / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development*; D002473:Cell Wall / Q000737:chemistry; D003208:Concanavalin A / Q000378:metabolism; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D006596:Hexosaminidases / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D008970:Molecular Weight; D010449:Peptide Mapping; D014415:Tunicamycin / Q000494:pharmacology
D005656:Fungal Proteins; D008562:Membrane Glycoproteins; D003208:Concanavalin A; D014415:Tunicamycin; D006596:Hexosaminidases