[Evaluation of [troponin-T] as a new biochemical marker for acute myocardial infarction].
Troponin (Tn) and tropomyosin (Tm) are located in the thin actin-containing filaments. Tn, a complex of three subunits (Tn-C, Tn-I and Tn-T), plays an important role in muscle contraction via calcium metabolism. In the present study, we evaluated the usefulness of a serum Tn-T assay in patients with acute myocardial infarction. The method, based on the sandwich immunoassay, was developed for the determination of serum Tn-T. The analytical precision data were good enough for routine analysis (CVs of repeatability and day-to-day reproducibility were between 3.2 to 6.2%). The values obtained on 60 healthy adults were below 0.2 ng/ml. Physical activity (mountain climbing and golf) increased serum total CK and myoglobin, but did not increase Tn-T. In myocardial infarction, serum Tn-T was up to 20 times higher than the cut off value at admission, and had a large peak at around 14 hr and another small peak at 40 to 55 hr after the onset. The ratio of Tn-T at around 14 and 32 hr (14/32) was an excellent marker for successful reperfusion. All cases of successful PTCA, had a ratio higher than 1.0.
Takagi
Y
Y
Department of Clinical Pathology, Showa University School of Medicine, Tokyo.
Gomi
K
K
D015415:Biomarkers / Q000097:blood*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D009203:Myocardial Infarction / Q000175:diagnosis*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D014336:Troponin / Q000097:blood*; D020107:Troponin T
D015415:Biomarkers; D014336:Troponin; D020107:Troponin T