[Improvement of pituitary homograft under the kidney capsule in mice].
The model of hyperprolactinemia induced by pituitary homografts under the kidney capsule has been used mainly in the field of reproductive physiology. The authors report an improved method for pituitary grafting in mice. The procedure was as follows: 1. The male pituitary glands with normal saline were aspirated into a polyethylene tube. 2. Two incisions were made in the kidney capsule. 3. The polyethylene tube with pituitary glands was inserted via a large incision. 4. Blowing air into the tube, the pituitary glands were left under the kidney capsule and normal saline streamed out of a small incision. Using this method, all pituitary grafted mice became pseudopregnant.
Aoki
S
S
Veterinary Medical Teaching Hospital, Tokyo, Japan.
Saito
T R
TR
Otaka
S
S
Amao
H
H
Tagawa
M
M
Umeda
M
M
Sugiyama
M
M
Takahashi
K W
KW
D000818:Animals; D005260:Female; D007668:Kidney; D008297:Male; D051379:Mice; D010902:Pituitary Gland / Q000637:transplantation*; D011555:Pseudopregnancy; D016042:Transplantation, Heterotopic; D014184:Transplantation, Homologous