The oligopeptide (Gly-Pro)2-Ala-(Gly-Pro)2 increases the internal proline level and improves NaCl tolerance when produced in Escherichia coli.
We have produced various proline-containing peptides as translational fusions with beta-glucuronidase (beta Glu) in Escherichia coli. When these linkers were introduced in the 5'-end of the beta Glu-encoding gene, the production of the enzyme was increased substantially in vivo. The peptides carrying repetitive Gly-Pro sequences could also stimulate the growth of the transformants in media with inhibitory concentrations of NaCl. Furthermore, the freezing tolerance could be improved.
Bülow
L
L
Department of Pure and Applied Biochemistry, Chemical Center, Lund, Sweden.
Mosbach
K
K
D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005615:Freezing; D015870:Gene Expression; D005798:Genes, Bacterial; D005966:Glucuronidase / Q000235:genetics* / Q000378:metabolism; D009842:Oligopeptides / Q000235:genetics* / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism*; D014170:Transformation, Genetic; D014882:Water-Electrolyte Balance
D009842:Oligopeptides; D011993:Recombinant Fusion Proteins; D012965:Sodium Chloride; D011392:Proline; D005966:Glucuronidase