Analysis of the promoter region of saf, a Streptomyces griseus gene that increases production of extracellular enzymes.
The product of the saf gene of Streptomyces griseus ATCC10137 mediated an increase in the production of several extracellular enzymes and retarded the formation of pigments and spores in Streptomyces [Daza et al., Mol. Gen. Genet. 222 (1990) 384-392]. A promoter upstream from saf was identified by subcloning a DNA fragment in the promoter probe pIJ486. Using the Escherichia coli-Brevibacterium lactofermentum promoter-probe shuttle vector, pULMJ51, we determined that the saf promoter region is also active in E. coli. The transcription start points (tsp) of the saf promoter in Streptomyces and E. coli have been determined using high-resolution S1 mapping. The tsp are at the same position in both microorganisms. Expression from the saf promoter region was negatively regulated by phosphate in Streptomyces, but not in E. coli. The amplification of the saf promoter lacking the saf coding region did not increase the production of extracellular enzymes and did not reduce sporulation or pigmentation in Streptomyces (i.e., it does not titrate out a putative repressor of the genes encoding extracellular enzymes). Several structural features of the saf promoter region and saf mRNA are studied in relation to the regulation of the saf gene expression.
Daza
A
A
Department of Ecology, Genetics and Microbiology, University of León, Spain.
Martín
J F
JF
Vigal
T
T
Gil
J A
JA
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004798:Enzymes / Q000096:biosynthesis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005809:Genes, Regulator / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010710:Phosphates / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D013305:Streptomyces griseus / Q000201:enzymology / Q000235:genetics*
D001426:Bacterial Proteins; D004798:Enzymes; D010710:Phosphates