Sequencing self-ligated PCR products using 3' over-hangs generated by specific cleavage of dUTP by uracil-DNA glycosylase.
Day
P J
PJ
Department of Oncology, Children's Hospital, Birmingham, UK.
Walker
M R
MR
D001483:Base Sequence / Q000235:genetics; D045647:DNA Glycosylases; D004270:DNA, Circular / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism*; D003856:Deoxyuracil Nucleotides / Q000378:metabolism*; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism; D011071:Poly T / Q000378:metabolism; D016133:Polymerase Chain Reaction; D051981:Uracil-DNA Glycosidase
D004270:DNA, Circular; D004277:DNA, Single-Stranded; D003856:Deoxyuracil Nucleotides; C027078:deoxyuridine triphosphate; D011061:Poly A; D011071:Poly T; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase