Testicular lesions and lymphocyte subpopulations in rats immunized with a soluble fraction of testicular homogenate.
A soluble fraction obtained from a testicular homogenate by precipitation with ammonium sulphate (ASPM) was emulsified with Freund's complete adjuvant (CFA) and injected into Wistar rats. At 50 days after the first immunization (total of three injections) the animals had developed moderate and multifocal testicular damage, characterized mainly by sloughing of the seminiferous epithelium. A delayed-type hypersensitivity response and circulating antibodies to ASPM were detected at different times with maximum levels at 50 days. The addition of Bordetella pertussis to the immunization did not increase the severity of the lesion but augmented the cellular and humoral immune response to ASPM. The phenotypic characterization of cells present in the lymph nodes draining from the site of immunization in animals injected with CFA alone (control group) revealed an increase in CD8+ T-cells and a low CD4/CD8 ratio. Conversely, rats immunized with CFA plus ASPM (experimental group) exhibited testicular damage and showed a significant decrease in CD8+ cells with a normal CD4/CD8 ratio. In conclusion, rats immunized with a testicular antigen developed focal aspermatogenic lesions and a concomitant specific immune response as well as lymph-node cell variations focused apparently on the CD8+ T-cell subpopulation.
Doncel
G F
GF
Centro de Investigaciones en Reproducciòn, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Denduchis
B
B
Lustig
L
L
D000645:Ammonium Sulfate; D000818:Animals; D000917:Antibody Formation; D001324:Autoantigens / Q000008:administration & dosage*; D016827:CD8 Antigens; D005620:Freund's Adjuvant / Q000008:administration & dosage; D006968:Hypersensitivity, Delayed / Q000276:immunology; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000276:immunology / Q000473:pathology*
D001324:Autoantigens; D016827:CD8 Antigens; D005620:Freund's Adjuvant; D000645:Ammonium Sulfate