[Changes in the lipid and protein components of thymus cell membrane during lipid peroxidation].
Nonenzymatic lipid peroxidation in thymus cell plasma membranes was studied. The composition of lipid and protein components, intensity of fluorescence of the membrane probes (1-anilinonaphthalene-8-sulfonate, 4-dimethylaminochalcon, eosin, pyronin and rhodamine), fluorescence polarization of tryptophan residues of membrane proteins and quenching by acrylamide of intrinsic fluorescence of proteins were determined. Induction of lipid peroxidation by the Fe(2+)-ascorbate system caused changes in the composition and structure of lipids. This was paralleled with changes in the structural-dynamic organization of membrane proteins, transition of some peripheral proteins to the water phase and increased solubilization of integral proteins by Triton X-100.
Dreval'
V I
VI
D000818:Animals; D002417:Cattle; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D005454:Fluorescence Polarization; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D017830:Octoxynol; D010743:Phospholipids / Q000378:metabolism; D011092:Polyethylene Glycols; D014364:Tryptophan / Q000378:metabolism
D003902:Detergents; D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids; D011092:Polyethylene Glycols; D008315:Malondialdehyde; D014364:Tryptophan; D017830:Octoxynol
Izmeneniia lipidnogo i belkovogo komponentov plazmaticheskikh membran timusa pri perekisnom okislenii lipidov.