Processing and statistical analysis of laser Doppler data applied to the assessment of systemic anti-inflammatory drugs.
Continuous laser Doppler measurements of methyl nicotinate-induced skin inflammation have been used to evaluate the activities of three oral non-steroidal anti-inflammatory drugs, indomethacin 50 mg (Indocid), tiaprofenic acid 100 mg (Surgam) and sodium acetylsalicylate 1 g (Catalgine). They were compared in a single-blind, randomized, intra-individual comparison (N = 16) versus placebo (lactose). One hour after each drug was ingested, four concentrations of methyl nicotinate were applied to the subject's forearms. Simultaneous skin blood flow (SBF) measurements were then carried out on the four tested zones, by use of four calibrated laser Doppler flowmeters. Computerized processing of recorded SBF levels provided data related to flow amplitude, kinetics and magnitude (area under the curve) of the reactions. A detailed statistical analysis was performed to establish the selectivity of this type of test and the following points were demonstrated: adjustment of SBF data to baseline did not improve precision, data had to be log-transformed before analysis, and magnitude data gave the best product discrimination. Under the conditions of this study, i.e. one hour after oral administration and for the indicated doses, the tested products could be classified, in terms of anti-inflammatory activity, as follows: Lactose less than Indomethacin 50 mg = Tiaprofenic acid 100 mg less than Sodium acetylsalicylate 1 g.
Duteil
L
L
Centre International de Recherches Dermatologiques Galderma (CIRD GALDERMA), Valbonne, France.
Queille
C
C
Poncet
M
M
Czernielewski
J
J
D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology*; D001241:Aspirin / Q000494:pharmacology; D003877:Dermatitis, Contact / Q000188:drug therapy / Q000209:etiology; D005260:Female; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D009539:Nicotinic Acids / Q000008:administration & dosage; D011422:Propionates / Q000494:pharmacology; D012867:Skin / Q000098:blood supply / Q000187:drug effects*; D014664:Vasodilation / Q000187:drug effects
D000894:Anti-Inflammatory Agents, Non-Steroidal; D009539:Nicotinic Acids; D011422:Propionates; C021270:tiaprofenic acid; C008473:methyl nicotinate; D001241:Aspirin; D007213:Indomethacin