Endothelin-1 is expressed and released by a human endothelial hybrid cell line (EA.hy 926).
A permanent vascular endothelial cell line, EA.hy 926, was shown to express endothelin-1 (ET-1) mRNA and to secrete big ET-1 and ET-1 into culture medium. The concentration of both big ET-1 and ET-1 was significantly increased in EA.hy 926 culture medium by phosphoramidon, a metalloproteinase inhibitor, suggesting that phosphoramidon sensitive protease(s) may be responsible for the degradation of ET-1 and big ET-1. EA.hy 926 cells responded to various regulators of ET-1 similarly as primary human vascular endothelial cells. The production of ET-1 was increased by thrombin and decreased by vasodilators such as atrial natriuretic peptide, brain natriuretic peptide and nitroprusside, and by 8-bromo cyclic GMP and papaverine. This continuous human endothelial hybrid cell line could facilitate studies of regulation of ET-1 production in human endothelial cells, which in primary cultures have limited replication potential.
Saijonmaa
O
O
Minerva Institute for Medical Research, Helsinki, Finland.
Nyman
T
T
Hohenthal
U
U
Fyhrquist
F
F
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D001483:Base Sequence; D002460:Cell Line; D016232:Endothelins / Q000235:genetics* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D010208:Papaverine / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology; D014471:Umbilical Veins; D014665:Vasodilator Agents / Q000494:pharmacology
D016232:Endothelins; D012333:RNA, Messenger; D014665:Vasodilator Agents; D015124:8-Bromo Cyclic Adenosine Monophosphate; D010208:Papaverine; D013917:Thrombin