[Amplification using one-sided polymerase chain reaction, cloning and determination of primary structure of 5'-untranslated cDNA segments coding the somatogenic growth hormone receptor from rat liver].
The method of ligation-mediated, single-sided polymerase chain reaction was applied for the amplification of 5'-untranslated regions of cDNA coding for somatogenic growth hormone receptor from rat liver. The products of polymerase chain reaction were cloned and sequenced. Two variants of 5'-untranslated sequences of growth hormone receptor cDNA corresponding to products of alternative splicing of pre-mRNA were found.
Pekhletskiĭ
R I
RI
Chernov
B K
BK
Rubtsov
P M
PM
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011986:Receptors, Somatotropin / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D012333:RNA, Messenger; D011986:Receptors, Somatotropin; D004247:DNA
Amplifikatsiia s pomoshch'iu odnonapravlennoĭ polimeraznoĭ tsepnoĭ reaktsii, klonirovanie i opredelenie pervichnoĭ struktury 5'-netransliruemykh uchastkov kDNK, kodiruiushcheĭ somatogennyĭ retseptorformona rosta iz pecheni krysy.