title: A new biochemical method for estimation of postmortem time.
abstract: Hypoxanthine (Hx) is formed by hypoxic degradation of adenosine monophosphate (AMP) and might be elevated due to antemortem hypoxia. However, it also increases after cessation of the life processes. Until now measurements of potassium in corpus vitreous humor have been used by forensic pathologists to determine postmortem time. In this study the influence of postmortem time and temperature on vitreous humor Hx and potassium levels were compared. Repeated sampling of vitreous humor was performed in 87 subjects with known time of death and diagnosis. The bodies were kept at either 5 degrees C, 10 degrees C, 15 degrees C or 23 degrees C. Hx was measured by means of HPLC and potassium by flame photometry. In 19 subjects from whom samples were obtained within 1.5 h after death, the normal level of Hx could be estimated to be 7.6 mumol/l and that of potassium to be 5.8 mmol/l. The spread of the potassium levels measured shortly after death was much greater than for the corresponding Hx levels. In the four temperature groups the Hx level increased 4.2, 5.1, 6.2 and 8.8 mumol/l per h, respectively, whereas the corresponding figures for potassium were 0.17, 0.20, 0.25 and 0.30 mmol/l per h. The vitreous humor concentration of both Hx and potassium increases fairly linearly after death. The slopes are steeper with increasing temperature. Since the scatter of the levels is greater for potassium than for Hx, the latter parameter seems to be better suited for the determination of time of death in cases without antemortem hypoxia, especially during the first 24 h.
authors.affiliation: Institute of Forensic Medicine, National Hospital, University of Oslo, Norway.
authors.forename: T O
authors.initials: TO
authors.lastname: Rognum
authors.forename: S
authors.initials: S
authors.lastname: Hauge
authors.forename: S
authors.initials: S
authors.lastname: Oyasaeter
authors.forename: O D
authors.initials: OD
authors.lastname: Saugstad
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D011180:Postmortem Changes; D011188:Potassium / Q000032:analysis; D012044:Regression Analysis; D013696:Temperature; D013997:Time Factors; D014822:Vitreous Body / Q000737:chemistry*
chemical_list: D007042:Hypoxanthines; D019271:Hypoxanthine; D011188:Potassium