A species-specific oligonucleotide DNA probe for the identification of Meloidogyne incognita.
A genomic library of Meloidogyne incognita Race 1 has been prepared in the bacteriophage lambda gt10 and screened for specific DNA sequences by hybridization with radio-isotope labelled total genomic DNA from a number of Meloidogyne species. One clone isolated (MR1 #15), although not totally species specific, clearly showed preferential hybridization to M. incognita. Following subcloning and sequencing of the 255 bp insert, four stretches of the sequence corresponding to oligonucleotides of approximately equal length (approximately 70 bp) were synthesized and examined for specificity. One of them, MR1 #15.2, showed the necessary specificity to be used as a diagnostic tool.
Chacon
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Division of Immunology, National Institute for Medical Research, Mill Hill, London, UK.
Parkhouse
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Robinson
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MP
Burrows
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PR
Garate
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T
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D015723:Gene Library; D008969:Molecular Sequence Data; D009348:Nematoda / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D015342:DNA Probes; D004247:DNA