title: Cell and molecular anatomy of nicotinic acetylcholine receptor subunits and calcitonin gene-related peptide in the rat vestibular system.
abstract: In this report we demonstrate the pattern of calcitonin gene-related peptide (CGRP) mRNA and immunoreactivity in the central and peripheral vestibular system of the rat, using a CGRP cRNA probe and a polyclonal CGRP antiserum. We present evidence that somata in all regions of efferent vestibular neurons contain CGRP based on the correspondence between in situ hybridization (mRNA) and immunohistochemistry (mRNA translation product). CGRP immunohistochemistry (CGRPi) and in situ hybridization confirm that CGRPi axons and terminals present in the vestibular neuroepithelium are efferent in origin. Immunoelectron microscopy revealed an extensive innervation of the afferent vestibular pathway by CGRPi terminals that was not limited to the primary afferent chalice, as previously reported by Tanaka et al. (Brain Res 1989;504:31-5). An efferent neuromodulatory role of CGRP can be inferred from the distribution of terminals found on the primary afferent fibers, and type I and type II hair cells. In addition, we present evidence that nicotinic acetylcholine receptor (nAChR) subunit mRNA is expressed by primary afferent cell bodies. On the basis of these data, a hypothetical molecular mechanism of vestibular efferent modulation of the primary afferent pathway is proposed.
authors.affiliation: Victor Goodhill Ear Center, Division of Head and Neck Surgery, UCLA School of Medicine 90024-1624.
authors.forename: P A
authors.initials: PA
authors.lastname: Wackym
authors.forename: P
authors.initials: P
authors.lastname: Popper
authors.forename: P H
authors.initials: PH
authors.lastname: Ward
authors.forename: P E
authors.initials: PE
authors.lastname: Micevych
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis* / Q000276:immunology; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D008958:Models, Molecular; D009475:Neurons, Afferent / Q000166:cytology / Q000648:ultrastructure; D009476:Neurons, Efferent / Q000166:cytology; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D011978:Receptors, Nicotinic / Q000032:analysis* / Q000648:ultrastructure; D014722:Vestibule, Labyrinth / Q000033:anatomy & histology / Q000737:chemistry* / Q000294:innervation / Q000648:ultrastructure
chemical_list: D015347:RNA Probes; D012333:RNA, Messenger; D011978:Receptors, Nicotinic; D015740:Calcitonin Gene-Related Peptide