title: Crystallization and preliminary X-ray diffraction studies of the spinach-chloroplast thioredoxin f.
abstract: Thioredoxins are low-molecular-mass proteins that function as hydrogen carriers in DNA synthesis and in the transformation of sulfur metabolites. They also act as regulatory proteins in the light-dependent enzyme activation during photosynthesis. F-type thioredoxin from spinach chloroplasts, a monomeric protein of 113 amino acid residues, has been found to specifically activate fructose-1,6-bisphosphatase and other key enzymes of CO2 assimilation. It has been crystallized in the monoclinic system, space group P2(1) with a = 30.6 A, b = 63.1 A, c = 31.6 A and beta = 110.7 degrees. The crystals are suitable for X-ray diffraction studies.
authors.affiliation: Department of Structural Biology, University of Basel, Switzerland.
authors.forename: J C
authors.initials: JC
authors.lastname: Génovésio-Taverne
authors.forename: Y
authors.initials: Y
authors.lastname: Jetzer
authors.forename: U
authors.initials: U
authors.lastname: Sauder
authors.forename: E
authors.initials: E
authors.lastname: Hohenester
authors.forename: C
authors.initials: C
authors.lastname: Hughet
authors.forename: J N
authors.initials: JN
authors.lastname: Jansonius
authors.forename: L
authors.initials: L
authors.lastname: Gardet-Salvi
authors.forename: P
authors.initials: P
authors.lastname: Schürmann
mesh_terms: D054479:Chloroplast Thioredoxins; D002736:Chloroplasts / Q000737:chemistry*; D003461:Crystallography; D010940:Plant Proteins / Q000737:chemistry*; D010944:Plants / Q000737:chemistry*; D013879:Thioredoxins; D014961:X-Ray Diffraction
chemical_list: D054479:Chloroplast Thioredoxins; D010940:Plant Proteins; D013879:Thioredoxins