Heterogeneity of synaptosomal membrane preparations from different regions of calf brain studied by partitioning and counter-current distribution.
1. Membranes obtained by lysis and Yeda-press treatment of synaptosomes (nerve endings) from cortex, caudateus nucleus, and hippocampal region of calf brain have been studied by partitioning within a liquid-liquid aqueous two-phase system consisting of water, dextran, Ficoll, and poly(ethylene glycol). 2. The partitioning of membranes was sensitive to the presence of a dextran-bound dye, Procion yellow HE-3G, in the lower phase. 3. The two-phase system was used for counter-current distribution to study the heterogeneity of the synaptic membranes from the three regions of the brain and to separate the membranes into fractions. 4. The obtained counter-current distribution profiles strongly depended on the region of the brain from which the membranes were isolated. 5. The membrane fractions obtained showed marked differences in their SDS electrophoresis pattern.
Cebrian Pérez
J A
JA
Department of Biochemistry, University of Lund, Sweden.
Muiño Blanco
M T
MT
Johansson
G
G
D000818:Animals; D001921:Brain / Q000166:cytology*; D002417:Cattle; D002458:Cell Fractionation; D004591:Electrophoresis, Polyacrylamide Gel; D012542:Scattering, Radiation; D013499:Surface Properties; D013570:Synaptic Membranes / Q000502:physiology*; D013574:Synaptosomes / Q000502:physiology*