Factors affecting production of the group A streptococcus bacteriocin SA-FF22.
Factors influencing the production of streptococcin A-FF22 (SA-FF22) in liquid media were examined. Despite good growth of the producer strain, no SA-FF22 was detected during incubation at 40 degrees C, at pH 7, in Brain Heart Infusion Broth or in Mg(2+)-supplemented media. Optimal SA-FF22 production occurred at 32 degrees C, at pH 6.7, in cultures in Tryptic Soy Broth supplemented with glucose 2.25% and yeast extract 1%. Under these conditions SA-FF22 remained cell-associated but could be extracted with acid.
Jack
R W
RW
Department of Microbiology, University of Otago, Dunedin, New Zealand.
Tagg
J R
JR
D000900:Anti-Bacterial Agents; D001430:Bacteriocins / Q000096:biosynthesis*; D003470:Culture Media; D005285:Fermentation; D006131:Growth Inhibitors / Q000494:pharmacology; D010452:Peptide Biosynthesis; D010455:Peptides; D013297:Streptococcus pyogenes / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D003470:Culture Media; D006131:Growth Inhibitors; D010455:Peptides; C016887:A-FF22