title: Contribution of exercising legs to the slow component of oxygen uptake kinetics in humans.
abstract: Rates of performing work that engender a sustained lactic acidosis evidence a slow component of pulmonary O2 uptake (VO2) kinetics. This slow component delays or obviates the attainment of a stable VO2 and elevates VO2 above that predicted from considerations of work rate. The mechanistic basis for this slow component is obscure. Competing hypotheses depend on its origin within either the exercising limbs or the rest of the body. To resolve this question, six healthy males performed light nonfatiguing [approximately 50% maximal O2 uptake (VO2max)] and severe fatiguing cycle ergometry, and simultaneous measurements were made of pulmonary VO2 and leg blood flow by thermodilution. Blood was sampled 1) from the femoral vein for O2 and CO2 pressures and O2 content, lactate, pH, epinephrine, norepinephrine, and potassium concentrations, and temperature and 2) from the radial artery for O2 and CO2 pressures, O2 content, lactate concentration, and pH. Two-leg VO2 was thus calculated as the product of 2 X blood flow and arteriovenous O2 difference. Blood pressure was measured in the radial artery and femoral vein. During light exercise, both pulmonary and leg VO2 remained stable from minute 3 to the end of exercise (26 min). In contrast, during severe exercise [295 +/- 10 (SE) W], pulmonary VO2 increased 19.8 +/- 2.4% (P less than 0.05) from minute 3 to fatigue (occurring on average at 20.8 min). Over the same period, leg VO2 increased by 24.2 +/- 5.2% (P less than 0.05). Increases of leg and pulmonary VO2 were highly correlated (r = 0.911), and augmented leg VO2 could account for 86% of the rise in pulmonary VO2.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Medicine, University of California, San Diego, La Jolla, 92093-0623.
authors.forename: D C
authors.initials: DC
authors.lastname: Poole
authors.forename: W
authors.initials: W
authors.lastname: Schaffartzik
authors.forename: D R
authors.initials: DR
authors.lastname: Knight
authors.forename: T
authors.initials: T
authors.lastname: Derion
authors.forename: B
authors.initials: B
authors.lastname: Kennedy
authors.forename: H J
authors.initials: HJ
authors.lastname: Guy
authors.forename: R
authors.initials: R
authors.lastname: Prediletto
authors.forename: P D
authors.initials: PD
authors.lastname: Wagner
mesh_terms: D000328:Adult; D001784:Blood Gas Analysis; D001794:Blood Pressure / Q000502:physiology; D002245:Carbon Dioxide / Q000097:blood; D004837:Epinephrine / Q000097:blood; D015444:Exercise / Q000502:physiology*; D006339:Heart Rate / Q000502:physiology; D006801:Humans; D007700:Kinetics; D007773:Lactates / Q000097:blood; D007866:Leg / Q000502:physiology*; D008168:Lung / Q000378:metabolism; D008297:Male; D009638:Norepinephrine / Q000097:blood; D010101:Oxygen Consumption / Q000502:physiology*; D011188:Potassium / Q000097:blood; D012039:Regional Blood Flow / Q000502:physiology
chemical_list: D007773:Lactates; D002245:Carbon Dioxide; D011188:Potassium; D009638:Norepinephrine; D004837:Epinephrine