Thrombopoietic factor enhances murine megakaryopoiesis induced by recombinant erythropoietin.
Partially purified thrombopoietic factor (TPF) potentiated in vitro murine megakaryopoiesis induced by recombinant human erythropoietin (rh-Epo). High doses (2.5-10 units) of rh-Epo generated a considerable number of megakaryocytes in a dose-dependent manner in a serum-free liquid culture, whereas low doses of rh-Epo (0.5-1 units) failed to generate megakaryocytes. The addition of high doses of rh-Epo and TPF caused a significant increase of megakaryocytes in comparison with high doses of rh-Epo alone. Furthermore, low doses of rh-Epo with TPF caused generation of a small number of megakaryocytes, although TPF alone did not generate megakaryocytes. In addition, TPF enhanced the acetylcholinesterase (Ach-E) activity of megakaryocytes induced by rh-Epo. The potentiating effects of both factors, rh-Epo and thrombopoietic factor, may play an important role in thrombocytopenic states in vivo as well as in in vitro megakaryopoiesis.
Nagasawa
T
T
Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan.
Satoh
K
K
Abe
T
T
D000110:Acetylcholinesterase / Q000096:biosynthesis; D000818:Animals; D002478:Cells, Cultured; D004357:Drug Synergism; D004921:Erythropoietin / Q000494:pharmacology*; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects*; D008533:Megakaryocytes / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology; D013921:Thrombocytopenia / Q000097:blood; D013926:Thrombopoietin / Q000302:isolation & purification / Q000494:pharmacology*
D011994:Recombinant Proteins; D004921:Erythropoietin; D013926:Thrombopoietin; D000110:Acetylcholinesterase