Increase of hemolysin expression in Escherichia coli by cloned DNA sequences of Serratia marcescens.
A genomic library of Sau 3AI-generated Serratia marcescens DNA fragments was constructed in Escherichia coli 5K harbouring plasmid pANN202-312. Two clones capable of increasing the external hemolytic activity of the parental strain were isolated and characterized. The recombinant plasmids purified from these clones (designated pPSF29 and pPSH18) contained approximately 30 and 20 Kb, respectively, of Serratia DNA. Introduction of either plasmid in Escherichia coli caused a marked increase in hemolysin production and a concomitant decrease in cell viability.
Díaz
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Departament de Microbiología, Facultat de Biología Universitat de Barcelona, Spain.
Juárez
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D002462:Cell Membrane / Q000737:chemistry; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics* / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000235:genetics*; D012706:Serratia marcescens / Q000235:genetics*
D006460:Hemolysin Proteins