title: Separation of fluorescent oligosaccharide derivatives by microcolumn techniques based on electrophoresis and liquid chromatography.
abstract: Various aldose oligosaccharides can be quantitatively derivatized into primary amines for subsequent reaction with fluorogenic reagents, such as 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde or 3-benzoyl-2-naphthaldehyde. Capillary electrophoresis (CE) and microcolumn liquid chromatography (LC), coupled with laser-induced fluorescence detection, were evaluated as a means of separating complex oligosaccharide mixtures. Whereas microcolumn LC and open-tubular CE appear confined in their utility to relatively small oligosaccharides, unprecedented results were obtained with polyacrylamide gel-filled capillaries on hydrolyzed malto-oligosaccharides and enzymatically degraded samples of chondroitin sulfate and hyaluronic acid.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington 47405.
authors.forename: J P
authors.initials: JP
authors.lastname: Liu
authors.forename: O
authors.initials: O
authors.lastname: Shirota
authors.forename: M
authors.initials: M
authors.lastname: Novotny
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004586:Electrophoresis / Q000379:methods*; D005453:Fluorescence; D009844:Oligosaccharides / Q000032:analysis*
chemical_list: D009844:Oligosaccharides