Chemiluminescence intensities and spectra of luminol oxidation by sodium hypochlorite in the presence of hydrogen peroxide.
Hydrogen peroxide amplifies the chemiluminescence in the oxidation of luminol by sodium hypochlorite. A linear relationship between concentration of hydrogen peroxide and light intensity was found in the concentration range 5 x 10(-8)-7.5 x 10(-6) mol/l. At 7.5 x 10(-6) mol/l H2O2 the chemiluminescence is amplified 550-fold. The chemiluminescence spectra of these reactions have a wavelength maximum at 431 nm independent of the concentration of hydrogen peroxide. The results indicate that hydrogen peroxide is a necessary component in the chemiluminescent oxidation of the luminol by sodium hypochlorite.
Arnhold
J
J
Institute of Biophysics, School of Medicine, University of Leipzig, Germany.
Mueller
S
S
Arnold
K
K
Grimm
E
E
D006861:Hydrogen Peroxide / Q000737:chemistry; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*; D008954:Models, Biological; D010084:Oxidation-Reduction; D010587:Phagocytosis / Q000502:physiology; D012973:Sodium Hypochlorite / Q000737:chemistry; D013053:Spectrophotometry
D008165:Luminol; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite