[A method of equalizing the level of DNA sequences of varying amount in a heterogeneous DNA mixture].
A method for the equalization of double-stranded DNA concentrations in the mixture which may be used for equalizing double-stranded cDNA concentrations involves thermal denaturation of the double-stranded DNA mixture followed by reassociation. The initial reassociation rate is Vi = Ki.(single-stranded DNA)2, and by the end of the process the concentrations of the unreassociated molecules for different DNAs should be approximately equal. Using hydroxylapatite chromatography one can separate single-stranded DNAs from double-stranded DNAs and carry out complete single-stranded DNAs reassociation. The new ratio of different double-stranded DNA concentrations would be almost 1.
Il'ichev
A A
AA
Kishchenko
G P
GP
Petrenko
V A
VA
Puzyrev
A T
AT
D002846:Chromatography, Affinity; D004247:DNA / Q000737:chemistry*; D010957:Plasmids
D004247:DNA
Metod vyravnivaniia soderzhaniia posledovatel'nosteĭ raznoĭ kopiĭnosti v geterogennoĭ smesi DNK.