title: Isolation and comparative mapping of a human chromosome 20-specific alpha-satellite DNA clone.
abstract: We have isolated and characterized a human genomic DNA clone (PZ20, locus D20Z2) that identifies, under high-stringency hybridization conditions, an alphoid DNA subset specific for chromosome 20. The specificity was determined using fluorescence in situ hybridization. Sequence analysis confirmed our previously reported data on the great similarity between the chromosome 20 and chromosome 2 alphoid subsets. Comparative mapping of pZ20 on chimpanzee and gorilla chromosomes, also performed under high-stringency conditions, indicates that the alphoid subset has ancestral sequences on chimpanzee chromosome 11 and gorilla chromosome 19. However, no hybridization was observed to chromosomes 21 in the great apes, the homolog of human chromosome 20.
authors.affiliation: Department of Human Genetics, Yale University School of Medicine, New Haven, CT.
authors.forename: A
authors.initials: A
authors.lastname: Baldini
authors.forename: N
authors.initials: N
authors.lastname: Archidiacono
authors.forename: R
authors.initials: R
authors.lastname: Carbone
authors.forename: A
authors.initials: A
authors.lastname: Bolino
authors.forename: V
authors.initials: V
authors.lastname: Shridhar
authors.forename: O J
authors.initials: OJ
authors.lastname: Miller
authors.forename: D A
authors.initials: DA
authors.lastname: Miller
authors.forename: D C
authors.initials: DC
authors.lastname: Ward
authors.forename: M
authors.initials: M
authors.lastname: Rocchi
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D002890:Chromosomes, Human, Pair 20; D004276:DNA, Satellite / Q000235:genetics*; D005453:Fluorescence; D006071:Gorilla gorilla / Q000235:genetics; D006801:Humans; D009693:Nucleic Acid Hybridization; D002679:Pan troglodytes / Q000235:genetics
chemical_list: D004276:DNA, Satellite