Determination of the amino acid sequence of an intramolecular disulfide linkage-containing sperm-activating peptide by tandem mass spectrometry.
A sperm-activating peptide (SAP) was isolated from the egg jelly of the sea urchin Stomopneustes variolaris. The presence of an intramolecular disulfide linkage in the peptide was demonstrated by fast atom bombardment (FAB) mass spectrometry with the intact and reduced peptides. The amino acid sequence of the reduced peptide was determined to be Lys-Phe-Cys-Pro-Glu-Gly-Lys-Cys-Val by tandem mass spectrometry from the spectrum produced by a collision-induced decomposition method. Furthermore, it was also demonstrated that SAPs obtained from sea urchins Arbacia punctulata and Glyptocidaris crenularis are cyclic peptides containing one cystine residue by FAB mass spectrometry.
Yoshino
K
K
Noto Marine Laboratory, Kanazawa University, Ishikawa, Japan.
Takao
T
T
Shimonishi
Y
Y
Suzuki
N
N
D000595:Amino Acid Sequence; D000818:Animals; D004220:Disulfides / Q000032:analysis* / Q000737:chemistry; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010063:Ovum / Q000737:chemistry; D010456:Peptides, Cyclic / Q000737:chemistry*; D012617:Sea Urchins
D004220:Disulfides; D010456:Peptides, Cyclic; C072072:sperm-activating peptide 1