Mitogenic effects of excitatory amino acids in the adult rat retina.
We studied the retinas of adult rats after the intravitreal injection of excitatory amino acids and ouabain. Kainic acid, domoic acid, N-methyl D-asparate and ouabain produced swelling and vacuolization of the outer plexiform, inner nuclear and inner plexiform layers and pyknosis. Mitoses were present in retinas treated with all agents other than N-methyl D-asparate. Rompun ketamine anesthesia blocked the mitogenic effects. Immunohistochemical labeling of both glial fibrillary acidic protein and S100 protein would indicate that the mitoses are occurring in glial cells. We suggest that the mitogenic effects are mediated through action on glial cationic channels, and might account for the reactive gliosis observed in some retinal lesions.
Sahel
J A
JA
David G. Cogan Eye Pathology Laboratory, Massachusetts Eye and Ear Infirmary, Boston 02114.
Albert
D M
DM
Lessell
S
S
Adler
H
H
McGee
T L
TL
Konrad-Rastegar
J
J
D000818:Animals; D002452:Cell Count; D004305:Dose-Response Relationship, Drug; D007608:Kainic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D008297:Male; D008938:Mitosis / Q000187:drug effects; D016202:N-Methylaspartate / Q000494:pharmacology*; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects; D010042:Ouabain / Q000494:pharmacology; D016318:Quisqualic Acid / Q000494:pharmacology*; D051381:Rats; D012160:Retina / Q000166:cytology*
D010042:Ouabain; D016202:N-Methylaspartate; D016318:Quisqualic Acid; C012301:domoic acid; D007608:Kainic Acid