Effects of exogenous putrescine on murine preimplantation development in vitro.
After first demonstrating that murine embryos take up putrescine from the medium in which they are cultured in vitro, fertilized eggs were placed in culture and maintained for 4 days in medium supplemented with varying amounts of putrescine. Their development was monitored each day. While embryos that were cultured in putrescine-supplemented medium developed at the same rate as control embryos, a significantly higher percentage of the putrescine-treated embryos attained the blastocyst stage as compared to the control group.
Sawicki
J A
JA
Lankenau Medical Research Center, Wynnewood, Pennsylvania 19096.
Impellizeri
A
A
O'Brien
T G
TG
D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D002970:Cleavage Stage, Ovum / Q000166:cytology / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004624:Embryo Transfer; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D011247:Pregnancy; D011700:Putrescine / Q000378:metabolism / Q000494:pharmacology*
D011700:Putrescine