Expression of mouse-GLUT3 and human-GLUT3 glucose transporter proteins in brain.
Polyclonal anti-peptide antisera were raised to the C-terminal sequence of mouse- and human-GLUT3 glucose transporter isoforms. GLUT3 protein and mRNA expression were investigated by Western blot and Northern blot assays, in a range of tissues and cell lines. Mouse-GLUT3 protein was detected only in rat brain, where it was present in most regions except adenohypophysis and pineal gland. Mouse-GLUT3 was also detected in primary cultured rat cerebellar neurons and the neuronal cell lines PC12 and NG108-15, but not in cultured astroglia. Human-GLUT3 protein was detected in CHO cells transfected with the human-GLUT3 cDNA and in human brain, but not in human placenta or fat. The expression of GLUT3 in rat brain and neurons indicates it is a major neuronal glucose transporter.
Maher
F
F
Experimental Diabetes, Metabolism and Nutrition Section, NIDDK, National Institutes of Health, Bethesda, MD 20892.
Vannucci
S
S
Takeda
J
J
Simpson
I A
IA
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D007106:Immune Sera; D051379:Mice; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D009928:Organ Specificity; D010455:Peptides / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D051381:Rats; D013045:Species Specificity
D007106:Immune Sera; D009004:Monosaccharide Transport Proteins; D010455:Peptides; D012333:RNA, Messenger