Strong priming of T cells adoptively transferred into scid mice.
We have examined the requirements for activating unprimed T cells in vivo by transferring T cells into scid mice, which lack mature B and T cells. Purified adult thymocytes and a protein antigen, keyhole limpet hemocyanin (KLH), were injected into scid mice. scid mice injected with T cells and KLH developed cellular lymph nodes containing CD4+ and CD8+ T cells. Cells recovered from the lymph nodes of injected scid mice proliferated and secreted interleukin 2 in response to KLH in vitro. The results indicate that T cells can be primed to KLH in the scid mouse in the absence of B cells.
Sunshine
G H
GH
Department of Surgery, Tufts University School of Veterinary Medicine, Boston, Massachusetts.
Jimmo
B L
BL
Ianelli
C
C
Jarvis
L
L
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology; D006433:Hemocyanins / Q000276:immunology; D016219:Immunotherapy, Adoptive; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin