title: Abnormal extracellular matrix and excessive growth of human adult polycystic kidney disease epithelia.
abstract: Human autosomal dominant polycystic kidney disease (ADPKD) epithelia were grown in primary monolayer cultures and their properties compared with intact kidney epithelial cultures derived from individually microdissected normal human kidney proximal convoluted tubules (PCT), proximal straight tubules (PST), and cortical collecting tubules (CCT). In vivo, ADPKD cyst epithelia exhibited a thickened basement membrane, and immunofluorescence demonstrated the presence of laminin, fibronectin, type IV collagen, and heparan sulfate proteoglycan in basement membranes and type I collagen in the interstitium. ADPKD epithelia grown in culture synthesized and secreted basally a unique, extracellular matrix that took the form of proteinaceous spheroids when the cells were grown on dried, type I collagen. Incorporation of H2[S35O4] into basement membrane extracts was increased more than ten-fold in ADPKD epithelia by comparison to normal PST and CCT. In addition to incorporation into the normal tubular basement membrane 220 kD band, radioactivity was also seen at 175 kD and 150 kD in ADPKD extracts. Growth in culture of cyst-lining ADPKD epithelia was more rapid than normal tubules, and was abnormal since there was no absolute requirement for added extracellular matrix. However, when ADPKD epithelia were grown on different, exogenous matrix protein components, a profound influence on both structure and epithelial cell proliferation was seen. Growth on a complete basement membrane three-dimensional gel derived from the Engelbreth-Holm-Swarm (EHS) sarcoma led to a reduction in the numbers of spheroids and increase in amorphous filaments. Incorporation of [3H]-thymidine into ADPKD epithelia was greater than into normal PCT, PST, and CCT and was also greatly modified by the type of extracellular matrix components provided. In studies using single matrix components, the strongest proliferative response was seen when ADPKD epithelia were plated on type I collagen greater than type IV collagen greater than fibronectin greater than laminin. These findings suggest that the excessive growth of cyst-lining epithelia may be, at least in part, a result of abnormal basement membrane and extracellular matrix production by ADPKD cells.
authors.affiliation: Department of Physiology and Biophysics, UMDNJ-Robert Wood Johnson, Medical School, Piscataway 08854-5635.
authors.forename: P D
authors.initials: PD
authors.lastname: Wilson
authors.forename: D
authors.initials: D
authors.lastname: Hreniuk
authors.forename: P A
authors.initials: PA
authors.lastname: Gabow
mesh_terms: D001485:Basement Membrane / Q000378:metabolism; D002455:Cell Division; D002478:Cells, Cultured; D004848:Epithelium / Q000473:pathology; D005109:Extracellular Matrix / Q000473:pathology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D066298:In Vitro Techniques; D007684:Kidney Tubules / Q000648:ultrastructure; D008854:Microscopy, Electron; D016891:Polycystic Kidney, Autosomal Dominant / Q000473:pathology*; D013431:Sulfates / Q000378:metabolism
chemical_list: D016326:Extracellular Matrix Proteins; D013431:Sulfates