Evaluation of a commercial measles virus immunoglobulin M enzyme immunoassay.
Paired serum samples from 93 patients suspected of having measles were assayed for measles virus-specific immunoglobulin M (IgM) antibodies by an enzyme immunoassay (EIA), and the results were compared with results from a complement fixation assay and an EIA for measles virus IgG. By using significant serologic rises as the standard for comparison, the IgM EIA assay had a sensitivity of 85.7%, a specificity of 81.3%, a positive predictive value of 95.7%, and a negative predictive value of 54.2%. This assay can be expected to perform well in outbreak situations.
Mayo
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DR
Biosciences Division, Connecticut State Department of Health Services, Hartford 06144.
Brennan
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Cormier
D P
DP
Hadler
J
J
Lamb
P
P
D003168:Complement Fixation Tests; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood*; D008297:Male; D008457:Measles / Q000175:diagnosis*; D008459:Measles virus / Q000276:immunology*; D011933:Reagent Kits, Diagnostic / Q000592:standards; D012680:Sensitivity and Specificity
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic