Suppressive effect of 1,25(OH)2D3, and glucocorticoids on production of tumor necrosis factor-alpha by human peripheral blood adherent cells.
Effect of 1,25(OH)2D3 and glucocorticoids on production of TNF-alpha by human peripheral blood adherent cells (HPBAC) was investigated. TNF-alpha was measured by ELISA method using monoclonal antibody against human recombinant THF-alpha. Maximal TNF-alpha production by HPBAC was observed in 4 to 8 hr after incubation when these cells were cultured for 24 hr with 10 micrograms/ml lipopolysaccharide (LPS), 10 ng/ml 12-o-tetradecanoyl-phorbol-13-acetate (TPA) and 10(-5) M indomethacin (INDM). When graded concentration of 1,25(OH)2D3 was added to HPBAC in the presence of above stated dose of LPS, TPA and INDM, a significant suppression of TNF-alpha production was first observed at the concentration of 10(-8) M (P less than 0.05). Similar significant suppressive effect was also observed by adding hydrocortisone and dexamethasone at the concentration of 10(-8) M (P less than 0.05) and 10(-7) M (P less than 0.05), respectively. These results may suggest that 1,25(OH)2D3 has the same inhibitory effect on monocyte/macrophage function as glucocorticoids.
Katakami
N
N
Department of Medicine, Kobe University School of Medicine, Japan.
Nakao
Y
Y
Fujita
T
T
D000328:Adult; D002117:Calcitriol / Q000494:pharmacology*; D002448:Cell Adhesion; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D005938:Glucocorticoids; D014409:Tumor Necrosis Factor-alpha; D002117:Calcitriol