Effects of ozone on cellular ATP levels in rat and mouse alveolar macrophages.
Hydrogen peroxide (H2O2) is thought to be a major intermediate in the toxicity of ozone. In a previous study we demonstrated that ATP depletion may play an important role in the H2O2-induced inhibition of the phagocytic functions of alveolar macrophages. Ozone exposure can adversely affect the phagocytic capacities of alveolar macrophages. In the present study we investigated whether a decrease in the cellular ATP concentration may underly the effects of ozone on alveolar macrophages. Neither following single (6 and 12 h) exposure nor repeated (12 h/day for 3 and 7 days) exposures of mice or rats to 0.4 ppm ozone, were decreased levels of ATP found in the alveolar macrophages. In contrast, repeated exposures of mice for 7 days to ozone led to a significant increase (1.5-fold) in the ATP content of the alveolar macrophages. In vitro ozone exposure of rat and mouse alveolar macrophages also did not lead to a decrease in the cellular ATP concentration. These results showed that ATP depletion does not play a role in the toxicity mechanism of ozone for alveolar macrophages.
Oosting
R S
RS
Laboratory of Toxicology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Van Rees-Verhoef
M
M
Verhoef
J
J
Van Golde
L M
LM
Van Bree
L
L
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002374:Catalase / Q000494:pharmacology; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D010126:Ozone / Q000633:toxicity*; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D010126:Ozone; D000255:Adenosine Triphosphate; D002374:Catalase