Resonance ionization spectroscopy for multiplex sequencing of tin-labeled DNA.
A method is described for synthesis of a tin reagent, triethylstannylpropanoic acid (TESPA), and its attachment to oligonucleotide primers. Except for the expected mobility retardation, the presence of [116Sn]-TESPA did not affect the sequencing ladder on electrophoresis gels. By using [120Sn]-TESPA and [35S]-dTTP simultaneously in the Sanger procedure, DNA bands on an electrophoresis gel were first located by autoradiography and then by resonance ionization spectroscopy to demonstrate the coincidence of the signals. Previous results using stable isotopes as labels on model compounds are now confirmed by their use in actual DNA sequencing products.
Sachleben
R A
RA
Chemistry Division, Oak Ridge National Laboratory, TN 37831.
Brown
G M
GM
Sloop
F V
FV
Arlinghaus
H F
HF
England
M W
MW
Foote
R S
RS
Larimer
F W
FW
Woychik
R P
RP
Thonnard
N
N
Jacobson
K B
KB
D001345:Autoradiography; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D007202:Indicators and Reagents; D007477:Ions; D007554:Isotopes; D013057:Spectrum Analysis; D014267:Triethyltin Compounds / Q000737:chemistry*
D007202:Indicators and Reagents; D007477:Ions; D007554:Isotopes; D014267:Triethyltin Compounds; D004247:DNA