Molecular cloning and sequence analysis of the mouse kallikrein-binding protein gene.
A genomic clone (MKBP-10) encoding the mouse kallikrein-binding protein (MKBP) was isolated from a mouse genomic DNA library by screening with a rat kallikrein-binding protein (RKBP) cDNA probe. The total sequenced region of the MKBP gene spans 8615 base pairs. The exon and intron locations of the RKBP gene were identified by similarity with the RKBP gene. The MKBP gene encodes a prepeptide of 417 amino acid residues which exhibits 71% homology with RKBP. A TATA box sequence was located in the 5' flanking region of the MKBP gene by similarity with the consensus sequence TATAAAA.
Chai
K X
KX
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29425-2211.
Chao
J
J
Chao
L
L
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics*; D007438:Introns / Q000235:genetics; D007610:Kallikreins / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics; D051381:Rats; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D015843:Serpins / Q000235:genetics*; D016385:TATA Box / Q000235:genetics
D002352:Carrier Proteins; D006023:Glycoproteins; D011498:Protein Precursors; D015843:Serpins; C051324:kallistatin; D007610:Kallikreins