Electrophoresis with intermittent scanning of the migration path: detection of resolution within shortened time.
Intermittent optical scanning (by detection of optical density or fluorescence) of the electrophoretic migration path was applied to the resolution of two dyes under an arbitrary set of conditions. Scanning at 5-min intervals allows for detection of resolution between the two zones at least 3 times faster than conventional automatic zone detection employing a detector at the end of the migration path. This result promises that replacing stationary by mobile detectors in general would result in a substantial time saving for automated detection of electrophoretic zones.
Deml
M
M
Section on Macromolecular Analysis, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
Garner
M M
MM
Chrambach
A
A
D003720:Densitometry / Q000295:instrumentation; D004586:Electrophoresis / Q000295:instrumentation*; D004867:Equipment Design; D013053:Spectrophotometry; D013997:Time Factors