An immunohistochemical study with an estrogen receptor-related protein (ER-D5) in human colorectal cancer.
The ER-D5 antigen is a protein associated with the estrogen receptor that is present only in estrogen receptor-positive tissues. To clarify the estrogen receptor in colon cancer, the localization of ER-D5 was studied immunohistochemically. Forty-three of 171 (25.1%) specimens of colon cancer showed reactivity for ER-D5. Reactivity was seen only in the cytoplasm. No reactivity was seen in cancer limited to the mucosa or in adenoma, but submucosal cancers showed a rate of positivity similar to cancers that involved lamina muscularis and beyond. Estradiol staining was done on serial sections from 56 specimens. There was a significant relationship between reactivity for estradiol and that for ER-D5. In the 15 specimens where both ER-D5 and estradiol were positive, the distribution of estradiol was similar to that of ER-D5. It was concluded that the immunohistochemical localization of ER-D5 indirectly shows that of the estrogen receptor in colorectal cancer tissues.
Takeda
H
H
Second Department of Internal Medicine, Yamagata University School of Medicine, Japan.
Yamakawa
M
M
Takahashi
T
T
Imai
Y
Y
Ishikawa
M
M
D000230:Adenocarcinoma / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000032:analysis; D015179:Colorectal Neoplasms / Q000378:metabolism*; D004958:Estradiol / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000378:metabolism; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D011506:Proteins / Q000032:analysis*; D011960:Receptors, Estrogen / Q000032:analysis*
D014408:Biomarkers, Tumor; C059917:ERD5 protein, human; D011506:Proteins; D011960:Receptors, Estrogen; D004958:Estradiol