Aminoglycosides modify the in vitro metachromatic reaction and murine generalized Shwartzman phenomenon induced by Salmonella minnesota R595 lipopolysaccharide.
Endotoxin-neutralizing activity may be an important property for antibiotics to be used in severe sepsis. Several antibiotics, belonging to different classes, were evaluated as to their endotoxin-neutralizing ability, using the inhibition of an in vitro metachromatic assay for lipopolysaccharides and a murine generalized Shwartzman reaction model. Gentamicin, amikacin, and sisomicin have been found to share significant in vitro antiendotoxin activity at an antibiotic/endotoxin ratio as low as 1.0/5 (by weight) and to reduce the murine generalized Shwartzman reaction at an antibiotic/endotoxin ratio of 3.3/5.
Focà
A
A
Cattedra di Microbiologia, Università di Reggio Calabria, Policlinico Mater Domini, Catanzaro, Italy.
Matera
G
G
Iannello
D
D
Berlinghieri
M C
MC
Liberto
M C
MC
D000617:Aminoglycosides; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D005260:Female; D008070:Lipopolysaccharides / Q000633:toxicity*; D008751:Methylene Blue; D051379:Mice; D012475:Salmonella / Q000378:metabolism*; D012790:Shwartzman Phenomenon / Q000517:prevention & control*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D008070:Lipopolysaccharides; D008751:Methylene Blue