Effect of anthelmintics on the antioxidant system of Nippostrongylus brasiliensis.
To understand the mode of anthelmintic action of thiabendazole and methyl-[5-[[4-(2-pyridinyl)-l-piperazinyl]carbonyl]-1H-benzimidazole- 2-yl] carbamate (C.D.R.I. compound 81/470) against Nippostrongylus brasiliensis, their effect on the metabolism of reactive oxygen species in the parasite as well as in rat intestine was examined. Both drugs produced a significant depression in the levels of superoxide dismutase (SOD) and reduced glutathione (GSH) of the parasite. Release of antioxidant enzymes by the drug-treated worms was also found to be appreciably lowered. Both thiabendazole and compound 81/470 induced a depression in the levels of all five constituents of the antioxidant system of rat intestine but significant alterations were detected only in the GSH content of infected and the SOD activity of normal intestine. The production of O2- by treated intestine was, on the other hand, markedly enhanced. Increased formation of O2- by the host intestine accompanied with the reduced level of SOD and GSH in N. brasiliensis appear to have a deleterious effect on the parasite. Consequently, the drug-treated worms are unable to retain themselves in situ and are ultimately expelled. The greater effect produced on these parameters by thiabendazole compared to compound 81/470 is consistent with the relative efficacy of these anthelmintics.
Srivastava
J K
JK
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Batra
S
S
Gupta
S
S
Katiyar
J C
JC
Srivastava
V M
VM
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism*; D001562:Benzimidazoles / Q000494:pharmacology; D002219:Carbamates / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects* / Q000469:parasitology; D008297:Male; D009349:Nematode Infections / Q000378:metabolism; D009559:Nippostrongylus / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D013827:Thiabendazole / Q000494:pharmacology
D000871:Anthelmintics; D000975:Antioxidants; D001562:Benzimidazoles; D002219:Carbamates; C043589:CDRI 81-470; D013827:Thiabendazole