title: [Modification of the global coagulation tests for the dog. Significance for the monitoring of heparin therapy].
abstract: When the global coagulation tests Quick's test, aPTT and thrombin time are carried out on dogs using reagents from the human medical sector according to the manufacturer's instructions, they react insensitively to deficiencies of the corresponding coagulation factors or to the presence of fibrin degradation products. In the present study it was attempted to develop sensitive global tests for the dog by modifying these tests. The Quick's test reacted apparently more sensitively after a 1:4 dilution of the test plasma with physiological saline solution. The sensitivity of the aPTT was demonstrated by the addition of BaSO4-adsorbed plasma to normal plasma. It seems to be particularly advantageous to combine a 1:3 plasma dilution with a reduction in the CaCl2-concentration to 5 mmol/l. Likewise, plasma dilution allows the thrombin time to react more sensitively in the presence of increased concentrations of fibrin degradation products. The aPTT and thrombin time are, as in human beings, suited for the control of a heparin therapy. However, since prolongation of the coagulation times is considerably dependent on the test chosen, the heparin sensitivity of the test should be determined in vitro prior to use.
authors.affiliation: Klinik für kleine Haustiere, Tierärztlichen Hochschule Hannover.
authors.forename: R
authors.initials: R
authors.lastname: Mischke
authors.forename: I
authors.initials: I
authors.lastname: Nolte
mesh_terms: D000818:Animals; D004285:Dogs / Q000097:blood*; D006493:Heparin / Q000008:administration & dosage / Q000627:therapeutic use*; D010314:Partial Thromboplastin Time / Q000662:veterinary*; D011517:Prothrombin Time / Q000662:veterinary*; D012016:Reference Values; D013918:Thrombin Time / Q000662:veterinary*
chemical_list: D006493:Heparin
vernacular_title: Modifikation der globalen Gerinnungstests für den Hund. Bedeutung für die Uberwachung der Heparintherapie.