title: Separation of peripheral blood lymphocytes with low platelet and erythrocyte contamination, using ADP aggregation.
abstract: A simple and inexpensive method to obtain substantially platelet-free and erythrocyte-free lymphocyte preparations from human peripheral blood has been established, using a modification of the technique published by Vives et al. (1971). Lymphocyte levels of up to 3 x 10(6) per ml blood have been obtained, with 2-3% platelet and erythrocyte contamination per ml blood, by pre-aggregating platelets with low concentrations of adenosine 5'diphosphate (ADP).
authors.affiliation: University Department of Pharmacology, Oxford, U.K.
authors.forename: L M
authors.initials: LM
authors.lastname: Castell
mesh_terms: D001792:Blood Platelets; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D002498:Centrifugation; D004912:Erythrocytes; D006801:Humans; D008214:Lymphocytes