Establishment and characterization of a new human Bence Jones-type myeloma cell line, NOP-2.
A new human myeloma cell line NOP-2, producing immunoglobulin (Ig)-lambda-light chain was established from a patient with Bence Jones-type multiple myeloma. Morphologically, the cell line had plasmacytoid characteristics by light- and electron-microscopic examination. Phenotypic studies of NOP-2 cells revealed no surface Ig, but they were positive for cytoplasmic Ig-lambda, OKT10 (CD 38), and PCA-1. Epstein-Barr nuclear antigen was not detected. Chromosomal abnormalities of t(11;14) and t(8;22) were found in both NOP-2 cells and the original myeloma cells obtained from the patient. NOP-2 cells produced and secreted Ig-lambda light chain, but lacked immunoglobulins of any heavy chains. Rearrangements of both immunoglobulin heavy- and light-chain genes were observed in NOP-2 cells, though the cells expressed detectable mRNA only for Ig-lambda light chain. This cell line may serve as a useful model for understanding the hierarchy of human immunoglobulins and the pathophysiology of Bence Jones-type multiple myeloma.
Nagai
T
T
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Ogura
M
M
Morishita
Y
Y
Okumura
M
M
Kato
Y
Y
Morishima
Y
Y
Hirabayashi
N
N
Ohno
R
R
Saito
H
H
D000951:Antigens, Neoplasm / Q000032:analysis; D001536:Bence Jones Protein; D014408:Biomarkers, Tumor / Q000032:analysis; D002880:Chromosomes, Human, Pair 11 / Q000648:ultrastructure; D002883:Chromosomes, Human, Pair 14 / Q000648:ultrastructure; D002892:Chromosomes, Human, Pair 22 / Q000648:ultrastructure; D002898:Chromosomes, Human, Pair 8 / Q000648:ultrastructure; D015322:Gene Rearrangement, B-Lymphocyte; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics / Q000473:pathology*; D010641:Phenotype; D012333:RNA, Messenger / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured / Q000648:ultrastructure
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D001536:Bence Jones Protein