title: High-performance capillary electrophoresis of histones.
abstract: A high-performance capillary electrophoresis (HPCE) system was developed for the fractionation of histones. This system involves electroinjection of the sample and electrophoresis in 0.1 M phosphate buffer (pH 2.5) in a 35 cm x 50 micron I.D. coated capillary. Electrophoresis was accomplished in 9 min, separating a whole histone preparation into its components in the following order of decreasing mobility: (MHP) H3, H1 (major variant), H1 (minor variant), (LHP) H3, (MHP) H2A (major variant), (LHP) H2A, H4, H2B and (MHP) H2A (minor variant), where MHP is the more hydrophobic component and LHP is the less hydrophobic component. This order of separation is very different from that found in acid-urea polyacrylamide gel electrophoresis and in reversed-phase high-performance liquid chromatography and, thus, brings the histone biochemist a new dimension for the qualitative analysis of histone samples.
authors.affiliation: Los Alamos National Laboratory, Life Sciences Division, NM 87545.
authors.forename: L R
authors.initials: LR
authors.lastname: Gurley
authors.forename: J E
authors.initials: JE
authors.lastname: London
authors.forename: J G
authors.initials: JG
authors.lastname: Valdez
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000737:chemistry / Q000166:cytology; D005591:Chemical Fractionation; D006224:Cricetinae; D004586:Electrophoresis / Q000379:methods*; D005260:Female; D006657:Histones / Q000032:analysis*; D006863:Hydrogen-Ion Concentration
chemical_list: D006657:Histones