Phosphorylation of lysophosphatidylinositol by carrot membranes.
sn-1 Palmitoyl lysophosphatidylinositol is found in carrot suspension culture cells and can be phosphorylated to [32P]lysophosphatidylinositol monophosphate (LPIP) when [gamma 32P]ATP is added to isolated membranes. Based on in vivo labeling studies, [3H]inositol sn-1 palmitoyl LPIP was found predominantly in the plasma membrane-rich fraction or upper phase isolated by aqueous two-phase partitioning and LPI was found in the intracellular membrane-rich fraction or lower phase (Wheeler and Boss, Plant Physiol. 85, 389-392, 1987). While both membrane fractions phosphorylated LPI in vitro, the apparent Km for LPI in the intracellular membrane fraction was 180 microM and for the plasma membrane was 580 microM. When cells were treated with the ionophore, monensin, the percentage of [3H]inositol LPIP increased in the whole cell lipid extract. However, the monensin treatment decreased the amount of [3H]inositol LPIP and PIP recovered in the plasma membrane fraction relative to the sum of the individual lipid, [3H]inositol LPIP or PIP, respectively, recovered in both membrane fractions.
Wheeler
J J
JJ
Department of Botany, North Carolina State University, Raleigh 27695.
Gross
W
W
Assefa
H
H
Boss
W F
WF
D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism; D007294:Inositol / Q000378:metabolism; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D008246:Lysophospholipids / Q000097:blood / Q000378:metabolism*; D008566:Membranes / Q000187:drug effects / Q000378:metabolism*; D008985:Monensin / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000097:blood / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism; D010766:Phosphorylation; D010945:Plants, Edible / Q000378:metabolism*
D004075:Diglycerides; D008246:Lysophospholipids; D010716:Phosphatidylinositols; C025449:lysophosphatidylinositol; C071981:sn-1-palmitoyl lysophophatidylinositol; C071982:sn-1-palmitoyl lysophosphatidylinositol monophosphate; D007294:Inositol; D008985:Monensin; D010741:Phospholipases A