Induction of arthritis in rats by soluble peptidoglycan-polysaccharide complexes produced by human intestinal flora.
Peptidoglycan-polysaccharide complexes were isolated from feces of a healthy subject and from ileostomy fluid from 5 patients. Peptidoglycan-polysaccharide complexes were tested for arthritogenicity in a rat model, by subcutaneous injection in Freund's incomplete adjuvant. Complexes from the healthy subject did not induce arthritis, but those from ileostomy fluid of 1 of the patients induced severe, chronic joint inflammation. We concluded that peptidoglycan-polysaccharide complexes from intestinal flora are potentially arthritogenic in rats. This arthritogenicity may be influenced by the content of muramic acid and rhamnose in these complexes.
Kool
J
J
Department of Immunology, Erasmus University, Rotterdam, The Netherlands.
Ruseler-van Embden
J G
JG
van Lieshout
L M
LM
de Visser
H
H
Gerrits-Boeye
M Y
MY
van den Berg
W B
WB
Hazenberg
M P
MP
D000328:Adult; D000368:Aged; D000818:Animals; D001168:Arthritis / Q000139:chemically induced*; D001644:Bifidobacterium / Q000378:metabolism*; D005051:Eubacterium / Q000378:metabolism*; D005260:Female; D006801:Humans; D007422:Intestines / Q000382:microbiology; D008875:Middle Aged; D009112:Muramic Acids / Q000032:analysis; D010457:Peptidoglycan / Q000009:adverse effects* / Q000032:analysis / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D012210:Rhamnose / Q000032:analysis
D009112:Muramic Acids; D010457:Peptidoglycan; D012210:Rhamnose