Determination of elastin-antielastin circulating immune complexes in serum of normal subjects and atherosclerotic patients.
Healthy subjects of different ages and a group of atherosclerotic patients were tested for the presence of elastin-antielastin circulating immune complexes (CIC) in their sera. For the purpose the authors used a method based on sequential precipitation with rising concentrations of polyethylene glycol (PEG), dissociation of the precipitated CIC and further analysis of the resuspended precipitates for the presence of elastin derived peptides by an enzyme-linked immunosorbent assay (ELISA). Elastin-antielastin CIC were detected only in the serum of atherosclerotic patients and that of healthy subjects over 60. The elastin-antielastin CIC obtained from the serum of atherosclerotic patients were precipitated by higher PEG concentrations mainly and had a higher elastin content in comparison with those isolated from the serum of healthy persons over 60.
Baydanoff
S
S
Department of Biology, University School of Medicine, Pleven, Bulgaria.
Nicoloff
A G
AG
D000293:Adolescent; D000328:Adult; D000368:Aged; D000936:Antigen-Antibody Complex / Q000032:analysis*; D001161:Arteriosclerosis / Q000175:diagnosis / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003937:Diagnosis, Differential; D004549:Elastin / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D012016:Reference Values
D000936:Antigen-Antibody Complex; D004549:Elastin