The 31 kd major allergen, Alt a I1563, of Alternaria alternata.
A component of Alternaria extract, previously identified as the major allergen, Alt a I1563, was purified to homogeneity from Alternaria mycelium by means of acetone precipitation and ion-exchange chromatography. The homogeneity of Alt a I1563 was assessed by one single band after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, by one single radiolabeled band after transfer to nitrocellulose, and by one single peak after size-exclusion chromatography by high-performance liquid chromatography. Alt a I1563 was isolated as a heat-stable acidic glycoprotein (carbohydrate content, 20%; pI, 4.0 to 4.5; 31 kd). The role of the carbohydrate moiety in the allergenicity is suggested. This major allergen is located in the cytoplasm of mycelium and spore.
Paris
S
S
Unité de Mycologie, Institut Pasteur, Paris, France.
Debeaupuis
J P
JP
Prévost
M C
MC
Casotto
M
M
Latgé
J P
JP
D000485:Allergens / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D000528:Alternaria / Q000737:chemistry / Q000276:immunology* / Q000648:ultrastructure; D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005656:Fungal Proteins / Q000032:analysis; D006801:Humans; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D012882:Skin Tests
D000485:Allergens; D000908:Antibodies, Fungal; D005656:Fungal Proteins; D007073:Immunoglobulin E