Detection of poly(ADP ribose) polymerase in crude extracts by activity-blot.
We have recently devised an activity-blot procedure permitting the detection, on the same nitrocellulose sheet, of the functional poly(ADP ribose) polymerase (PARP) activity as well as the immunostained active peptide(s) after renaturation of the transferred protein(s). Using this technique we have analyzed the PARP activity in higher and lower eukaryotes directly on crude extracts from cell cultures. This procedure has been extended also to in situ screening of bacterial colonies expressing the PARP enzymatic activity.
Simonin
F
F
Institut de Biologie Moléculaire et Cellulaire du CNRS, Université Louis Pasteur, Strasbourg, France.
Briand
J P
JP
Muller
S
S
de Murcia
G
G
D002478:Cells, Cultured; D005057:Eukaryotic Cells / Q000201:enzymology; D015151:Immunoblotting; D066298:In Vitro Techniques; D011065:Poly(ADP-ribose) Polymerases / Q000032:analysis*; D014020:Tissue Extracts / Q000032:analysis
D014020:Tissue Extracts; D011065:Poly(ADP-ribose) Polymerases