Characterization of muscarine receptors in rabbit ciliary body smooth muscle using propylbenzilylcholine mustard.
1. A piece of rabbit ciliary body was mounted in organ bath and used as a test organ containing muscarine receptors. 2. A concentration response curve of carbachol was completely inhibited by a 50 min treatment of the smooth muscle preparation with propylbenzilylcholine mustard(PrBCM; 10(-6) M). This treatment was reported to block PrBCM-sensitive muscarine receptors in intestinal smooth muscle completely. 3. It is suggested that an existance of only PrBCM-sensitive muscarinic receptors, though there are two subtypes of muscarine receptors; PrBCM-sensitive and -resistant ones in intestinal smooth muscles.
Takayanagi
I
I
Department of Chemical Pharmacology, Toho University School of Pharmaceutical Sciences, Chiba, Japan.
Saito
K
K
Koike
K
K
Satoh
M
M
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002924:Ciliary Body / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism*; D011438:Propylbenzilylcholine Mustard / Q000494:pharmacology*; D011817:Rabbits; D011976:Receptors, Muscarinic / Q000187:drug effects*
D011976:Receptors, Muscarinic; D011438:Propylbenzilylcholine Mustard; D002217:Carbachol