[Glycoforms of serum alpha 1-antichymotrypsin studied by immunoaffino-electrophoresis. From the fundamental aspect to clinical applications].
alpha 1-antichymotrypsin, purified or in whole serum, exhibits microheterogeneous forms when studied by crossed immunoaffinoelectrophoresis with free concanavalin A (Con A) in the first dimension. alpha 1-antichymotrypsin purified from the serum of a single healthy donor was separated into three forms by affinity chromatography on a Con A-Sepharose 4B column: a Con A non-reactive form, a Con A weakly reactive form and a Con A reactive form. Some of their physico-chemical properties are compared. The complete primary structure of the glycans of each form was determined by high resolution 1H-NMR spectroscopy. The results indicated the presence of diantennary and triantennary type glycanic structures which occur frequently in serum glycoproteins. From deglycosylation experiments it is concluded that alpha 1-antichymotrypsin carries four oligosaccharide side chains. The Con A non-reactive form contains four triantennary glycans, the weakly reactive form contains three triantennary and one diantennary glycans and the Con A reactive form possesses on average one triantennary and three diantennary glycans. Significant variations in the relative ratios of the microheterogeneous forms were detected in various inflammatory syndromes. There is an increased proportion of Con A non-reactive form in patients developing a systemic disease (systemic lupus erythematosus, rheumatoid arthritis, temporal arteritis). In contrast, the proportion of Con A non-reactive form decreased in patients with acute septic inflammation whereas no variation appeared in patients with metastatic breast cancer. We also studied the variations of alpha 1-antichymotrypsin microheterogeneity in sera from patients with giant-cell arteritis and/or polymyalgia rheumatica before and during treatment with glucocorticoids.(ABSTRACT TRUNCATED AT 250 WORDS)
Laine
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A
Unité de l'Institut National de la Santé et de la Recherche Médicale, Lille.
Hachulla
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E
D000368:Aged; D000369:Aged, 80 and over; D002846:Chromatography, Affinity / Q000379:methods; D005260:Female; D013700:Giant Cell Arteritis / Q000097:blood / Q000150:complications; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D007123:Immunoelectrophoresis, Two-Dimensional / Q000379:methods*; D007249:Inflammation / Q000097:blood; D008297:Male; D008875:Middle Aged; D011111:Polymyalgia Rheumatica / Q000097:blood / Q000150:complications; D000514:alpha 1-Antichymotrypsin / Q000097:blood* / Q000737:chemistry*
D000514:alpha 1-Antichymotrypsin
Etude des glycoformes de l' alpha 1-antichymotrypsine sérique en immunoaffino-électrophorèse. De l'aspect fondamental aux applications cliniques.