[Group determination of 17-oxosteroids on thin-layer chromotography].
17-oxosteroids from human urine are measured by thin layer chromatography (Merck Kieselgel F-254 plates) with fluorometric detection at 310 nm (densitometer SHIMADZU CS-9000): 11-OH-etiocholanolone, 11-OH-androsterone + oxoetiocholanolone, oxoandrosterone, dehydroepiandrosterone and androsterone. Fluorescence of hydrazone derivatives after the reaction of steroids with dansylhydrazine represents the principle of the method. The recoveries of steroids added to human urine samples are 97-101.3% and the coefficients of variation are 0.78-11.77.
Tomsová
Z
Z
III. interní klinika 1. lékarské fakulty Univerzity Karlovy, Praha.
Jůzová
Z
Z
D015068:17-Ketosteroids / Q000652:urine*; D002855:Chromatography, Thin Layer; D006801:Humans
D015068:17-Ketosteroids
Skupinové stanovení 17-oxosteroidů chromatografií na tenké vrstvĕ.