Seven-base-pair inverted repeats in DNA form stable hairpins in vivo in Saccharomyces cerevisiae.
Palindromic sequences in single-stranded DNA and RNA have the potential for intrastrand base pairing, resulting in formation of "hairpin" structures. We previously reported a genetic method for detecting such structures in vivo in the yeast Saccharomyces cerevisiae. Below, we describe evidence indicating that a 14-base-pair palindrome (7 bp per inverted repeat) is sufficient for formation of a hairpin in vivo.
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Department of Biology, University of North Carolina, Chapel Hill 27599-3280.
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D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics*; D006860:Hydrogen Bonding; D008540:Meiosis; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004271:DNA, Fungal