Human tumor-infiltrating lymphocytes transfected with tumor necrosis factor gene could augment cytotoxicity to autologous tumor cells.
Human tumor-infiltrating lymphocytes (TILs) derived from pleural or ascitic fluid were incubated with recombinant interleukin 2 and transfected with human tumor necrosis factor (TNF) alpha gene by the lipofection procedure. The resulting TILs secreted significant amounts of TNF in the culture supernatant and exhibited cytotoxicity against established cell lines, such as K562 and Daudi, and autologous tumor cells. The TNF gene-transfected TILs exhibited an augmented killing of autologous tumor cells.
Itoh
Y
Y
Department of Internal Medicine (Section 4), Sapporo Medical College.
Kohgo
Y
Y
Watanabe
N
N
Kanisawa
Y
Y
Sakamaki
S
S
Takahashi
M
M
Hirayama
Y
Y
Ono
H
H
Himeno
T
T
Niitsu
Y
Y
D001201:Ascites / Q000276:immunology; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008175:Lung Neoplasms / Q000276:immunology*; D016246:Lymphocytes, Tumor-Infiltrating / Q000187:drug effects / Q000276:immunology*; D010996:Pleural Effusion / Q000276:immunology; D011994:Recombinant Proteins / Q000494:pharmacology; D013274:Stomach Neoplasms / Q000276:immunology*; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000502:physiology*
D007376:Interleukin-2; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha