Studies on adherence and outer membrane protein of enteropathogenic Escherichia coli 0127: H6 and their related plasmids.
The following characteristics were found in 20 epidemic strains of enteropathogenic Escherichia coli (EPEC) 0127: H6 isolated from an outbreak of neonatal diarrhea: 1) Absent Vero toxin production; 2) No potential for invasiveness; 3) Possession of 1.5 and 60 Md plasmid identical restriction digest and outer membrane protein (OMP) patterns; 4) Ability of localized adherence to HEp-2, HeLa and FL cells; 5) Capability to cause diarrhea in rabbits with destruction of the ileal microvilli at the areas of bacterial adherence. After elimination of the 60 Md plasmid from EPEC 0127: H6 the 45 and 82 Kd OMPs of the parent strain were lost. These plasmid-cured strains became non-adherent to HEp-2, HeLa and FL cells, and unable to cause diarrhea in rabbits. These results suggest that the pathogenic mechanism of EPEC 0127: H6 induced diarrhea may be related to the genes on a 60 Md plasmid expressed as 45 and 82 Kd OMPs which cause localized adherence to epithelial cells and destruction of ileal microvilli. This damage leads to a marked reduction in absorptive surface area, resulting in diarrhea.
Wu
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Department of Pediatrics, Chongqing Medical University, Children's Hospital, China.
Peng
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D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D001427:Bacterial Toxins / Q000096:biosynthesis; D002478:Cells, Cultured; D003967:Diarrhea / Q000382:microbiology; D004768:Enterotoxins / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology; D010957:Plasmids; D011817:Rabbits; D022622:Shiga Toxin 1
D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; D022622:Shiga Toxin 1