title: Aluminium uptake by intestinal cells: effect of iron status and precomplexation.
abstract: The investigation of different aluminium-binding agents and metabolic conditions that might act as critical factors in modulating aluminium absorption could yield valuable information in the understanding of aluminium gastrointestinal absorption. We evaluated the effect of iron depletion in aluminium uptake and the role of transferrin and citrate in aluminium incorporation by the intestinal epithelial cell line RIE1. Both complex, aluminium-citrate and aluminium-transferrin were prepared in a molar ratio of 2:1; both facilitated the aluminium uptake although a greater aluminium incorporation was found when aluminium was administered as aluminium-transferrin. This difference became even greater in the iron-depleted cells (normal cells vs iron-depleted cells): 7.7 +/- 1.4 versus 30 +/- 5.6 ng Al/micrograms DNA (P less than 0.05). From these and previous data it is possible to speculate that iron status could modulate the intestinal uptake of aluminium and that both transferrin and citrate would act as effective carriers in the incorporation of aluminium into mucosal cells.
authors.affiliation: Bone and Mineral Research Unit, Hospital General de Asturias, Universidad de Oviedo, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Fernández Menéndez
authors.forename: G S
authors.initials: GS
authors.lastname: Fell
authors.forename: J H
authors.initials: JH
authors.lastname: Brock
authors.forename: J B
authors.initials: JB
authors.lastname: Cannata
mesh_terms: D000535:Aluminum / Q000493:pharmacokinetics*; D000818:Animals; D001693:Biological Transport, Active; D002460:Cell Line; D002951:Citrates / Q000378:metabolism; D019343:Citric Acid; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D014168:Transferrin / Q000378:metabolism
chemical_list: D002951:Citrates; D014168:Transferrin; D019343:Citric Acid; D000535:Aluminum; D007501:Iron