Ion-sparing diuresis by 2,3-dibenzylbutane-1,4-diol, a synthetic mammalian-lignan derivative.
Diuretic properties of a synthetic lignan, 2,3-dibenzylbutane-1,4-diol (hattalin), and a naturally occurring arctigenin were examined in BALB/c male mice and Wistar male rats. Intra peritoneal administration of hattalin (50 mg/kg) in mice increased urine volume by 1.7-3.1 fold that of placebo-treated animals 40-260 min after administration (p less than 0.05 vs control). In contrast, 100 mg/kg of arctigenin had no effect on urine volume in mice. Hattalin (100 mg/kg), arctigenin (100 mg/kg), or furosemide (50 mg/kg) as a positive control was administered orally to rats, and accumulated urine volume was measured for up to 6-12 h. The urine volume of animals administered with hattalin showed 1.4-1.5 fold that of placebo-treated animals after 2-6 h of administration (P less than 0.05, n = 10). On the other hand, arctigenin showed no significant effect on urine volume for up to 12 h after administration (n = 8). The urine volume in animals administered with furosemide (n = 10) was 2.0-3.0 fold that of placebo-treated animals (P less than 0.01). Furosemide increased total Na+, K+, or Cl- excretion by 1.9, 1.8 or 2.2 fold, respectively, when compared with placebo-treated controls (P less than 0.01), whereas hattalin decreased Na+ excretion by 3.6 times (P less than 0.01), K+ excretion by 1.4 times (not significant), and Cl- excretion by 3.1 times (P less than 0.01). Serum Na+ and K+ levels did not change in both furosemide- and hattalin-administered rats, however, serum Cl- levels in these animals significantly decreased (P less than 0.01) when compared with controls. The results suggest that the diuretic property of hattalin is due to a novel mechanism which is different from that of furosemide or other diuretics modifying the ion-exchange at the uriniferous tubules.
Hirano
T
T
Division of Clinical Pharmacology, Tokyo College of Pharmacy, Japan.
Homma
M
M
Oka
K
K
Naito
T
T
Hosaka
K
K
Mitsuhashi
H
H
D000818:Animals; D001593:Benzyl Compounds / Q000494:pharmacology*; D002712:Chlorides / Q000097:blood / Q000652:urine; D004232:Diuretics / Q000494:pharmacology*; D005663:Furans / Q000494:pharmacology; D005665:Furosemide / Q000494:pharmacology; D007474:Ion Exchange; D017705:Lignans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009318:Natriuresis / Q000187:drug effects; D011188:Potassium / Q000097:blood / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000097:blood
D001593:Benzyl Compounds; D002712:Chlorides; D004232:Diuretics; D005663:Furans; D017705:Lignans; C063012:2,3-dibenzylbutane-1,4-diol; D005665:Furosemide; D012964:Sodium; D011188:Potassium; C071942:arctigenin