title: Immunological identification and sequence characterization of a peptide derived from the processing of neuroendocrine protein 7B2.
abstract: A newly raised antiserum against the C-terminal region of neuroendocrine protein 7B2 was used to purify a novel peptide from the culture media of the mouse corticotroph cell line AtT-20. Based on partial sequencing, this peptide, which we call Cter-7B2, begins at Ser156 and appears to result from the cleavage of pro7B2 after a five-basic-residue sequence. Thus, 7B2 processing may contribute to the diversity of peptides found in neuronal and endocrine cells.
authors.affiliation: Institut de Recherches Cliniques de Montréal, Université de Montréal, Québec, Canada.
authors.forename: L
authors.initials: L
authors.lastname: Paquet
authors.forename: N
authors.initials: N
authors.lastname: Rondeau
authors.forename: N G
authors.initials: NG
authors.lastname: Seidah
authors.forename: C
authors.initials: C
authors.lastname: Lazure
authors.forename: M
authors.initials: M
authors.lastname: Chrétien
authors.forename: M
authors.initials: M
authors.lastname: Mbikay
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001483:Base Sequence; D014408:Biomarkers, Tumor; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D051859:Neuroendocrine Secretory Protein 7B2; D010446:Peptide Fragments / Q000276:immunology / Q000302:isolation & purification; D010907:Pituitary Hormones / Q000096:biosynthesis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011499:Protein Processing, Post-Translational; D011863:Radioimmunoassay
chemical_list: D000906:Antibodies; D014408:Biomarkers, Tumor; D009419:Nerve Tissue Proteins; D051859:Neuroendocrine Secretory Protein 7B2; D010446:Peptide Fragments; D010907:Pituitary Hormones