Region of hepatitis B virus DNA with a homology to the yeast ARS replication enhancer.
A computer analysis of the primary sequence of hepatitis B virus (HBV) subtype ayw DNA, cloned within the pVG2 recombinant plasmid, which raises its stability in Saccharomyces cerevisiae transformants, was performed. This revealed that the structure of the HBV DNA has: two bends in the termination regions of the HBs and HBc genes, and multiple sequences with a high degree of homology to the ARS (autonomously replicating sequence) core consensus in this region of the HBs gene. DNA fragments from the HBs region (330 bp) and from the HBc region (378 bp) have an abnormal electrophoretic mobility in 8% polyacrylamide gels. The similarity of the structural motifs in the stop-region of HBs gene with the B-domain of the S. cerevisiae ARS element is discussed.
Legchilina
S P
SP
Institute of Molecular Genetics, Academy of Sciences of the USSR, Moscow.
Efimenko
I G
IG
Shlyakhtenko
L S
LS
Arman
I P
IP
D001483:Base Sequence; D004261:DNA Replication; D004279:DNA, Viral / Q000737:chemistry*; D016322:HIV Enhancer; D006515:Hepatitis B virus / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral