Application of polymyxin-coated polyester cloth to the semi-quantitation of Salmonella in processed foods.
A rapid and economical semi-quantitative test for Salmonella cells in foods is proposed. Food samples containing different levels of Salmonella cells were homogenized and serially diluted in enrichment broths and then incubated for about 20 h at 37 degrees C. The presence of Salmonella cells in each dilution was assayed by capturing deoxycholate-extracted Salmonella lipopolysaccharides on a sheet of polymyxin-coated polyester cloth, followed by colorimetric detection with an anti-Salmonella antibody-enzyme conjugate. The minimum dilutions which resulted in no detectable growth were correlated with the extent of Salmonella contamination in the food samples.
Blais
B W
BW
Department of Biology, Carleton University, Ottawa, Ontario, Canada.
Yamazaki
H
H
D003124:Colorimetry; D005511:Food Handling; D005516:Food Microbiology; D007124:Immunoenzyme Techniques; D008070:Lipopolysaccharides / Q000032:analysis*; D011112:Polymyxin B; D012486:Salmonella typhimurium / Q000254:growth & development* / Q000302:isolation & purification
D008070:Lipopolysaccharides; D011112:Polymyxin B