Perforin is expressed in CTL populations generated in vivo.
Immunization of C57BL/6 mice with syngeneic tumor cells, MBL-2, resulted in the generation of antitumor effector cells in vivo. The immunized C57BL/6 mice permanently rejected viable MBL-2 lymphoma cells, but not B16 melanoma cells. Cytotoxic T cells obtained from MBL-2-immunized mouse peritoneal cells (PEC) showed specific cytotoxicity against MBL-2, but not to YAC-1, RDM-4 and Meth A cells. By sorting with FACStar, the specific CTL were characterized as TCR alpha beta+ CD8+ T cells. Moreover, the cytoplasm of in vivo-induced CTL was stained with a monoclonal antibody against perforin. The localization of perforin in cytoplasmic granules of CTL was demonstrated by electron microscope analysis. This experiment presented the first evidence that in vivo-induced CD8+ CTL against syngeneic tumor cells expressed significant amounts of perforin.
Takeuchi
Y
Y
Department of Immunology, Tokai University School of Medicine, Isehara, Japan.
Nishimura
T
T
Gao
X H
XH
Watanabe
K
K
Akatsuka
A
A
Shinkai
Y
Y
Okumura
K
K
Habu
S
S
D000818:Animals; D016827:CD8 Antigens; D003593:Cytoplasm / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D005260:Female; D007114:Immunization; D016399:Lymphoma, T-Cell / Q000276:immunology; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000276:immunology
D016827:CD8 Antigens; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D052899:Pore Forming Cytotoxic Proteins; D054353:Perforin