title: Potentiometric enzyme channeling immunosensor for proteins.
abstract: A potentiometric immunosensor for the detection of human IgG has been developed using an asymmetric, ion-selective membrane with immobilized adenosine deaminase and IgG. A protein A-alkaline phosphatase conjugate binds to the immobilized IgG, creating a bienzymatic catalytic layer. In the presence of sample IgG, the conjugate does not bind to the membrane. Instead, the intermediate in the two-step reaction (adenosine) must diffuse to the membrane surface, reducing the rate of product (ammonium) formation within the diffusion layer detected by the membrane. The immunosensor demonstrated is for the determination of IgG. A simplified model is described to predict the maximum rate enhancement for the 'channeled' versus 'unchanneled' reaction mechanisms.
authors.affiliation: Department of Chemistry, University of Michigan, Ann Arbor 48109-1055.
authors.forename: D V
authors.initials: DV
authors.lastname: Brown
authors.forename: M E
authors.initials: ME
authors.lastname: Meyerhoff
mesh_terms: D015374:Biosensing Techniques; D004800:Enzymes, Immobilized; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D008567:Membranes, Artificial; D011199:Potentiometry
chemical_list: D004800:Enzymes, Immobilized; D007074:Immunoglobulin G; D008567:Membranes, Artificial