title: Crystallization of and preliminary X-ray data for the negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa.
abstract: The negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa has been purified from an over-expressing clone and crystalized. Crystals of diffraction quality were obtained from polyethylene glycol 4000 and ammonium sulphate. AmiC crystallizes in P4(2)2(1)2 (a = 104.4 A, c = 66.6 A) with one subunit in the asymmetric unit. Crystals diffract beyond 2.8 A.
authors.affiliation: Department of Biochemistry and Molecular Biology, University College London, U.K.
authors.forename: S A
authors.initials: SA
authors.lastname: Wilson
authors.forename: N E
authors.initials: NE
authors.lastname: Chayen
authors.forename: A M
authors.initials: AM
authors.lastname: Hemmings
authors.forename: R E
authors.initials: RE
authors.lastname: Drew
authors.forename: L H
authors.initials: LH
authors.lastname: Pearl
mesh_terms: D000581:Amidohydrolases / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D005798:Genes, Bacterial; D005809:Genes, Regulator; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D016366:Open Reading Frames; D009876:Operon; D033902:Periplasmic Binding Proteins; D011487:Protein Conformation; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*; D012097:Repressor Proteins / Q000737:chemistry; D015183:Restriction Mapping; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D033902:Periplasmic Binding Proteins; D012097:Repressor Proteins; C070135:AmiC protein, Pseudomonas aeruginosa; D000581:Amidohydrolases