title: Protein composition of crevicular fluid before and after treatment.
abstract: Crevicular fluid (CF) analysis is a potential tool for site-specific diagnosis of periodontal disease activity. In this study, CF was collected using a novel washing method from 91 sites in 18 adult periodontitis patients both before and after conventional periodontal treatment. The sites studied were classified according to their clinical status and the number of polymorphonuclear leukocytes (PMN's) in CF samples. CF proteins were analyzed from individual sites with gel electrophoresis (SDS-PAGE). Furthermore, both the cell-bound and soluble neutral proteolytic activities of the samples were determined. Albumin was the main protein both in healthy and slightly inflamed sites. The most severely inflamed sites were characterized by high levels of low molecular weight (LMW) proteins (mol. weight ca. 12,000) and strong cell-bound neutral proteolytic activity. Scaling and root planing reduced both the LMW proteins and neutral proteolytic activity markedly in pockets responding well to treatment. The levels of the LMW proteins in CF correlated with the cell-bound neutral proteolytic activity, which reflected the number of PMN's in the sample. The present results suggest that the appearance of the LMW proteins in CF is associated with the periodontal inflammatory status of the site.
authors.affiliation: Institute of Dentistry, University of Turku, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Mäkelä
authors.forename: E
authors.initials: E
authors.lastname: Söderling
authors.forename: K
authors.initials: K
authors.lastname: Paunio
authors.forename: J
authors.initials: J
authors.lastname: Talonpoika
authors.forename: T
authors.initials: T
authors.lastname: Hyyppä
mesh_terms: D000328:Adult; D000418:Albumins / Q000032:analysis; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry* / Q000166:cytology; D005891:Gingivitis / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009504:Neutrophils / Q000473:pathology; D010447:Peptide Hydrolases / Q000032:analysis; D010514:Periodontal Pocket / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D010518:Periodontitis / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D011506:Proteins / Q000032:analysis*; D014168:Transferrin / Q000032:analysis
chemical_list: D000418:Albumins; D007074:Immunoglobulin G; D011506:Proteins; D014168:Transferrin; D010447:Peptide Hydrolases