Application of capillary zone electrophoresis to the characterization of multiple antigen peptides.
Capillary zone electrophoresis (CZE) was applied to the analysis of a recently described class of synthetic branched peptides, multiple antigen peptides (MAPs). In comparison with high-performance liquid chromatography (HPLC), CZE showed superior resolution of minor impurities in samples of MAPs obtained by preparative chromatography. MAPs that differed only in the substitution of uncharged residues (Ala-Ser) could be separated by the addition of organic solvents (acetonitrile, methanol, ethanol) to the aqueous background electrolyte.
Pessi
A
A
Peptide Synthesis Unit, Sclavo SpA, Rome, Italy.
Bianchi
E
E
Chiappinelli
L
L
Nardi
A
A
Fanali
S
S
D000595:Amino Acid Sequence; D000941:Antigens; D002197:Capillary Action; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis* / Q000737:chemistry / Q000276:immunology
D000941:Antigens; D010455:Peptides