Electrohydrodynamic effects in continuous flow electrophoresis.
We demonstrate experimentally and theoretically the importance of electrohydrodynamic (EHD) flows in continuous-flow electrophoresis (CFE) separations. These flows are associated with variations in the conductivity or dielectric constant, and are quadratic in the field strength. They appear to be the main cause of extraneous and undesired flows in CFE which have degraded separation performance and have until now not been explained. We discuss the importance of EHD flows relative to other effects. We also describe possible techniques for reducing the associated degradation of CFE separations.
Rhodes
P H
PH
National Aeronautics and Space Administration, George C. Marshall Space Flight Center, Huntsville, Alabama 35812.
Snyder
R S
RS
Roberts
G O
GO
Baygents
J C
JC
D002021:Buffers; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D007202:Indicators and Reagents; D008433:Mathematics
D002021:Buffers; D007202:Indicators and Reagents