[Rapid time-resolved fluoroimmunometric analysis of myoglobin].
High performance quantitative procedure for evaluation of myoglobin in blood serum is developed. The principle of two-site immunometry was used involving monoclonal antibodies against nonoverlapping epitopes of myoglobin molecule. The fluorescent compound containing europium served as a label of the antibody. Modification of the single-step assay enabled to increase the velocity of the assay to about 30 min and simplify it. The assay allowed estimation of myoglobin over a broad range of concentrations from 2 ng up to 1,000 ng/ml. The assay may be recommended for use in clinical practice for express diagnosis of myocardium infarction.
Gracheva
E A
EA
Nechaeva
M V
MV
Roshke
V V
VV
Rykova
E Iu
EIu
Shishkin
G V
GV
Bredikhin
A V
AV
D005455:Fluorescent Antibody Technique; D006801:Humans; D009203:Myocardial Infarction / Q000175:diagnosis; D009211:Myoglobin / Q000097:blood*
D009211:Myoglobin
Bystryĭ vremiarazreshennyĭ fliuoroimmunometricheskiĭ analiz na mioglobin.