Sources of electrical transients in tectal neuropil of the frog, Rana pipiens.
We have studied the outer neuropil layers in frog tectum where the unmyelinated optic nerve fibers terminate. At any point in the neuropil an extracellular microelectrode records several different visually evoked electrical transients, distinct by size and shape. When classified by shape alone, each transient falls into one of 3 distinct classes. Some of these transients are binocularly driven, as originally described by Finch and Collett. The aggregate of the receptive fields of all the elements recorded at a single point defines a multiunit receptive field (MURF). Each MURF is characteristically oval, and divided into 3 sections along its long axis. Each section represents the aggregate of the receptive fields associated with one class of transient; i.e. transients belonging to only one specific class can be evoked by stimulating that part of the visual field corresponding to the appropriate section of the MURF. All of the MURFs mapped by recording in a single tectum are radially arranged in visual space about a central point, or 'visual pole'. Several conclusions are made. First, the two larger types of transient are generated postsynaptically by electrically active dendritic elements, specifically the beaded dendritic appendages of tectal neurons. The smallest type of transient is of presynaptic origin. Second, these tectal elements have a local and global anatomical order across the tectum, which accounts for both the tripartite structure of the MURFs and their radial arrangement about a visual pole. Third, since the large transients are of postsynaptic origin, genuine recordings of single retinal ganglion cell (RGC) activity can be made only in the optic nerve or retina itself. Fourth, information is conveyed over the unmyelinated optic nerve fibers at pulse rates as high as 80/s and is transsynaptically effective at such rates. Finally, the electrically active tectal dendritic elements, with their highly organized spatial arrangement, are an important component of the frog's visual processing apparatus, instead of being merely relays or repeaters.
Grant
A C
AC
Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge 02139.
Lettvin
J Y
JY
D000818:Animals; D003712:Dendrites / Q000648:ultrastructure; D004558:Electric Stimulation; D004594:Electrophysiology; D006056:Golgi Apparatus / Q000648:ultrastructure; D009412:Nerve Fibers / Q000502:physiology; D009900:Optic Nerve / Q000502:physiology; D011894:Rana pipiens / Q000502:physiology*; D012165:Retinal Ganglion Cells / Q000502:physiology; D013477:Superior Colliculi / Q000502:physiology* / Q000648:ultrastructure