[Studies of the expression of human interleukin-2 receptor cDNA in mammalian cells].
Human interleukin-2 receptor cDNA was transfected into mouse fibroblast cells (L929) by using DNA-calcium phosphate coprecipitation method. Results from RNA dot-blot hybri dization, FITC-IL-2 fluorescent staining assay and anti-Tac specific rossette test demonstrated that the product of human interleukin-2 receptor cDNA in L929 cells is able to bind IL-2 and anti-Tac antibody. Moreover, the abnormal expression of IL-2R gene in T-cell leukemia cell lines such as Jukat cells and Molt-4 cells was analyzed and discussed.
Zou
Q M
QM
Institute of Genetics, Fudan University, Shanghai.
Cai
W C
WC
Shen
W H
WH
Zhu
X H
XH
Li
C B
CB
Zhao
S Y
SY
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006801:Humans; D051379:Mice; D009693:Nucleic Acid Hybridization; D015282:Octreotide; D015375:Receptors, Interleukin-2 / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
D015375:Receptors, Interleukin-2; D004247:DNA; D015282:Octreotide