Demonstration of a human epsilon-globin gene silencer with studies in transgenic mice.
The human epsilon-globin gene displays normal developmental regulation in transgenic mice; it is expressed only in embryonic and in definitive erythroid cells. We show here that deletion of a negative element located between -182 and -467 bp upstream of the epsilon-globin gene cap site results in continuation of epsilon gene expression in the definitive erythroblasts of the fetal liver and in the red blood cells of adult transgenic mice. These data provide direct in vivo evidence that cis acting silencing elements are involved in the developmental control of the epsilon-globin gene.
Raich
N
N
Department of Medicine, University of Washington, Seattle 98195.
Papayannopoulou
T
T
Stamatoyannopoulos
G
G
Enver
T
T
D000818:Animals; D002872:Chromosome Deletion; D004900:Erythroblasts / Q000378:metabolism; D005312:Fetal Blood / Q000378:metabolism; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000166:cytology / Q000196:embryology; D051379:Mice; D008822:Mice, Transgenic
D005914:Globins