A simple method for the purification of mitochondrial DNA from Plasmodium falciparum.
Mitochondrial DNA (mtDNA) from Plasmodium falciparum was isolated by conventional differential centrifugation in an SS34 rotor, a simpler method than CsC1 centrifugation of total DNA as employed by other workers. The nature of the sample was verified by sequencing a polymerase chain reaction (PCR) product obtained using oligonucleotide primers derived from known malarial mtDNA sequence.
Flores
M V
MV
School of Biochemistry, University of New South Wales, Kensington, Australia.
Stewart
T S
TS
O'Sullivan
W J
WJ
D000818:Animals; D001483:Base Sequence; D002498:Centrifugation; D004272:DNA, Mitochondrial / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000737:chemistry
D004272:DNA, Mitochondrial; D016054:DNA, Protozoan; D012335:RNA, Ribosomal