A fluorometric enzyme immunoassay for follitropin and lutropin.
The analytical performance of the Stratus (Baxter) automatic analyser for human lutropin and follitropin determination was evaluated and compared with that of an immunoradiometric assay. Within-run and between-run imprecision were lower than those of the immunoradiometric assay. No significant differences were obtained in the parallelism study. A good and significant correlation was obtained between both methods. Results obtained for both methods were not interchangeable. The Stratus automatic analyser can replace radioisotopic methods, thus eliminating radioactive hazards.
Huguet
J
J
Sección de Hormonas (Servicio de Bioquímica), Hospital Princeps d'Espanya, Barcelona, España.
Bonnin
M R
MR
Guillén
E
E
Navarro
M A
MA
D001774:Blood Chemical Analysis / Q000379:methods / Q000592:standards / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D015200:Fluoroimmunoassay / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D005640:Follicle Stimulating Hormone / Q000097:blood* / Q000592:standards; D006801:Humans; D015592:Immunoradiometric Assay; D007986:Luteinizing Hormone / Q000097:blood* / Q000592:standards; D012015:Reference Standards; D012680:Sensitivity and Specificity
D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone