Characterization of the cytoskeleton in human normal and otosclerotic osteoblast-like cells.
The localization and distribution of some cytoskeletal protein components were studied by immunostaining methods in normal and ostosclerotic osteoblast-like cells. The protein components investigated were microtubules (beta-tubulin), intermediate filaments (vimentin), microfilaments (actin and myosin) and structural proteins (alpha-actinin and fibronectin). Although the mechanism is not yet clear, the alterations observed in the pathological cells could well play a role in the expression of otosclerosis.
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Istituto di Istologia ed Embriologia generale, Università di Sassari, Italia.
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Becchetti
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Donti
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Paludetti
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Bodo
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D000185:Actinin / Q000032:analysis; D000199:Actins / Q000032:analysis; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000737:chemistry*; D005260:Female; D005353:Fibronectins / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D009218:Myosins / Q000032:analysis; D010006:Osteoblasts / Q000737:chemistry* / Q000648:ultrastructure; D010040:Otosclerosis / Q000378:metabolism / Q000473:pathology*; D014404:Tubulin / Q000032:analysis; D014746:Vimentin / Q000032:analysis
D000199:Actins; D005353:Fibronectins; D014404:Tubulin; D014746:Vimentin; D000185:Actinin; D009218:Myosins