In vitro induction of perforin mRNA and cytotoxic activities in human splenocytes by the streptococcal preparation, OK-432.
Mononuclear cells were isolated from a spleen excised during surgery and cultured with 0.05 KE (Klinische Einheit)/ml of the streptococcal preparation, OK-432, or 700 JRU (Japan Reference Units)/ml of recombinant interleukin-2 (rIL-2). When the cytotoxicity against K562 and Daudi cells was measured by four-hour 51Cr release assay, OK-432 induced about the same killing activity as that of rIL-2. The peak activity was observed on day 2. When the expression of human perforin messenger RNA (mRNA) was investigated by Northern blot analysis, a low-grade expression was seen in the splenocytes shortly after isolation (day 0). An expression 2.7-fold stronger than that seen on day 0 was produced after one day of culturing with OK-432. This enhanced expression decreased only slightly after the first day. The increases in expression of perforin mRNA on day 2 were similar for OK-432 and rIL-2. The results indicate the multicytokine inducer, OK-432, to induce perforin to about the same degree as rIL-2 does, and perforin to act as one of the effector molecules in the killing activity induced by OK-432.
Kataoka
K
K
First Department of Surgery, Okayama University Medical School.
Naomoto
Y
Y
Orita
K
K
D003602:Cytotoxicity, Immunologic; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D016131:Lymphocyte Subsets; D008214:Lymphocytes / Q000276:immunology*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics*; D054353:Perforin; D010844:Picibanil / Q000494:pharmacology*; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger / Q000096:biosynthesis*; D011994:Recombinant Proteins; D013154:Spleen / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic
D007376:Interleukin-2; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D054353:Perforin; D010844:Picibanil