Retinal polyol and myo-inositol in galactosemic dogs given an aldose-reductase inhibitor.
Galactitol and myo-inositol concentrations were measured in retinas, erythrocytes, and skeletal muscle of experimentally galactosemic dogs receiving a placebo or the aldose reductase inhibitor, sorbinil, for 5 yr. The concentration of galactitol was increased more than 30-fold in the retina and other tissues by galactosemia, and the increase was inhibited 90-96% in all tissues by sorbinil. The concentration of free myo-inositol was greater than normal in retinas of galactosemic dogs, and its concentration was not altered by the aldose-reductase inhibitor. The myo-inositol concentration likewise was greater than normal in the retinas of dogs that were diabetic for 2-4 months. The marked inhibition of polyol production and accumulation in the retina of sorbinil-treated galactosemic dogs was not associated with a comparable inhibition of retinopathy.
Kern
T S
TS
Department of Ophthalmology, University of Wisconsin-Madison, WI 53706-1532.
Engerman
R L
RL
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors; D000496:Alloxan; D000818:Animals; D002849:Chromatography, Gas; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D004195:Disease Models, Animal; D004285:Dogs; D004912:Erythrocytes / Q000378:metabolism; D004376:Galactitol / Q000378:metabolism*; D005693:Galactosemias / Q000378:metabolism*; D007093:Imidazoles / Q000494:pharmacology; D048289:Imidazolidines; D007294:Inositol / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism; D012160:Retina / Q000378:metabolism*
D007093:Imidazoles; D048289:Imidazolidines; D004376:Galactitol; D007294:Inositol; D000496:Alloxan; D000449:Aldehyde Reductase; C026411:sorbinil