Typing of Proteus mirabilis from clinical sources by computerized analysis of electrophoretic protein patterns.
Seventy-five strains of Proteus mirabilis (mainly of human origin) were characterized by one-dimensional SDS-PAGE of cellular proteins. The strains came from various countries; 24 were from urine, 17 from faeces (including one from a dog and one from a monkey), 12 from the respiratory tract (including nine from sputum), four from a cerebral abscess, 12 from miscellaneous sources and six from unknown sources. The protein patterns, which contained 45 to 50 discrete bands, were highly reproducible. The patterns of the 75 P. mirabilis cultures plus those of the type strains of six Proteus and Providencia species were used as the basis for two numerical analyses. In the first, which included all the protein bands, the P. mirabilis cultures formed nine clusters at the 85% S level. In the second analysis, in which the principal protein bands (in the 34.0-44.6 kDa range) were excluded, 74 of the 75 cultures of P. mirabilis formed a single cluster at the 77% S level distinct from the six Proteus and Providencia reference strains. The P. mirabilis strain which failed to cluster with the others had a background band pattern typical of the species in the lower molecular weight region but appeared to be less typical in the heavier bands. It is concluded that high resolution PAGE combined with computerized analysis of protein patterns provides the basis for typing clinical strains of P. mirabilis. Reference strains of each of the nine PAGE types identified are available from NCTC for inclusion in future studies.
Holmes
B
B
National Collection of Type Cultures, Central Public Health Laboratory, London, UK.
Costas
M
M
Wood
A C
AC
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry; D001437:Bacteriuria / Q000382:microbiology; D001922:Brain Abscess / Q000382:microbiology; D016000:Cluster Analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology; D006801:Humans; D008970:Molecular Weight; D011512:Proteus Infections / Q000382:microbiology*; D011513:Proteus mirabilis / Q000737:chemistry / Q000145:classification*; D015203:Reproducibility of Results; D012137:Respiratory System / Q000382:microbiology; D013183:Sputum / Q000382:microbiology
D001426:Bacterial Proteins