Effect of ischemia and reperfusion in vivo on energy metabolism of rat sciatic-tibial and caudal nerves.
Our model of severe nerve ischemia consistently results in extinction of the compound nerve and muscle action potentials (NAP; CMAP) within 30 min. Since impulse transmission may depend on nerve energy metabolism (NEM), we studied the effects of ischemia with reperfusion on sciatic-tibial nerve NEM in vivo and compared these results with NEM of this nerve in deoxygenated Ringer's solution in vitro and postmortem. Ischemia for 30 min postmortem or in deoxygenated Ringer's solution resulted in marked depletion of adenosine triphosphate (ATP) and creatine phosphate (CP) and an increase in lactate (LAC) of sciatic-tibial nerve of adult male Sprague-Dawley rats. In vivo ischemia for up to 3 h to sciatic-tibial nerve was sufficient to extinguish CMAP but not NAP and did not deplete ATP, CP, or GLU nor did it increase LAC. Ischemia sufficient to extinguish NAP resulted in reduction of energy substrates to about 50% of resting. Muscle fails to conduct impulses before nerve and in vivo reductions of energy substrates are milder than in vitro changes. These changes are explainable in terms of energy requirements and supply. These findings support an energetic basis of ischemic conduction failure.
Zollman
P J
PJ
Department of Neurology, Mayo Foundation, Rochester, Minnesota 55905.
Awad
O
O
Schmelzer
J D
JD
Low
P A
PA
D000200:Action Potentials; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004734:Energy Metabolism; D007511:Ischemia / Q000378:metabolism* / Q000503:physiopathology; D007773:Lactates / Q000378:metabolism; D008297:Male; D010525:Peripheral Nerves / Q000098:blood supply / Q000378:metabolism* / Q000503:physiopathology; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015424:Reperfusion; D012584:Sciatic Nerve / Q000098:blood supply / Q000378:metabolism* / Q000503:physiopathology; D013979:Tibial Nerve / Q000098:blood supply / Q000378:metabolism* / Q000503:physiopathology
D007773:Lactates; D010725:Phosphocreatine; D000255:Adenosine Triphosphate