An 85-kilodalton surface antigen gene family of Trypanosoma cruzi encodes polypeptides homologous to bacterial neuraminidases.
We have determined the sequence of a cDNA (Tt34c1) encoding a Trypanosoma cruzi trypomastigote stage-specific 85-kDa surface glycoprotein (gp85). Within the peptide sequence of Tt34c1 are two 8-amino acid motifs, Ser-X-Asp-X-Gly-X-Thr-Trp, that are characteristic of bacterial neuraminidases. Analysis of the Tt34c1 sequence predicts the presence of an amino-terminal signal sequence and a hydrophobic carboxy-terminus that is probably replaced by a glycosyl phosphatidylinositol membrane anchor. Gp85 is encoded by an extensive multigene family that is distributed throughout the genome and can be divided into subsets on the basis of oligonucleotide hybridisation patterns. By sequencing products of polymerase chain reaction (PCR) amplification of the 5' end of trypomastigote gp85 mRNA we show that multiple copies of the gene family are transcribed simultaneously in a trypanosome population. Comparison of the sequence of the PCR clones and another gp85 cDNA showed a highly conserved region 5' of the first methionine extending 180 nt into the coding sequence. Insertions and point mutations were observable outside these homologous sequences demonstrating the variant nature of the gp85 mRNAs.
Takle
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Laboratory of Molecular Parasitology, Rockefeller University, New York 10021.
Cross
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D000595:Amino Acid Sequence; D000818:Animals; D000940:Antigenic Variation; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D016054:DNA, Protozoan / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D009439:Neuraminidase / Q000235:genetics*; D010455:Peptides / Q000235:genetics* / Q000276:immunology; D012333:RNA, Messenger / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000276:immunology; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000235:genetics* / Q000276:immunology; D014709:Vero Cells
D001426:Bacterial Proteins; D016054:DNA, Protozoan; D010455:Peptides; D012333:RNA, Messenger; D014643:Variant Surface Glycoproteins, Trypanosoma; D009439:Neuraminidase