title: The complete amino acid sequence of bovine serum amyloid protein A (SAA) and of subspecies of the tissue-deposited amyloid fibril protein A.
abstract: Bovine serum amyloid A (SAA) was isolated from the acute phase high density lipoprotein (HDL) fraction of a cow suffering from acute mastitis. The elucidated primary structure revealed a protein consisting of 112 amino acid residues. Compared with SAA proteins from other species, the bovine protein was shown to have an insertion of nine amino acid residues between positions 69 and 70. No microheterogeneity could be observed in the protein. Amyloid fibrils extracted from the kidneys were found to contain at least three subspecies of protein AA, consisting of 68, 81 and about 110 amino acid residues. The amino acid sequences established for the protein AA subspecies revealed no microheterogeneity, and were identical to that elucidated for protein SAA.
authors.affiliation: Department of Biochemistry, University of Oslo, Norway.
authors.forename: K
authors.initials: K
authors.lastname: Rossevatin
authors.forename: P K
authors.initials: PK
authors.lastname: Andresen
authors.forename: K
authors.initials: K
authors.lastname: Sletten
authors.forename: A
authors.initials: A
authors.lastname: Husebekk
authors.forename: G
authors.initials: G
authors.lastname: Husby
authors.forename: K
authors.initials: K
authors.lastname: Nordstoga
authors.forename: K H
authors.initials: KH
authors.lastname: Johnson
authors.forename: G T
authors.initials: GT
authors.lastname: Westermark
authors.forename: P
authors.initials: P
authors.lastname: Westermark
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007668:Kidney / Q000737:chemistry; D008075:Lipoproteins, HDL / Q000737:chemistry; D008414:Mastitis, Bovine / Q000097:blood; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D000685:Serum Amyloid A Protein / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D008075:Lipoproteins, HDL; D010446:Peptide Fragments; D000685:Serum Amyloid A Protein