[Transport of the hydroxy analogs of leucine in the brush border membrane vesicles of the rabbit small intestine].
Hydroxy analogues of essential amino acids can be used in clinical nutrition to minimize nitrogen intake. In this study intestinal uptake of L-leucine hydroxy analogue into rabbit jejunal brush-border membrane vesicles was investigated. An inward directed H(+)-gradient was a driving force of uptake (pHoutside = 6.0; pHinside = 7.5) and led to a transient accumulation. The saturable system has a apparent transport constant Kt = 15.4 mM. By trans stimulation experiments it could be shown that both D- and L-stereoisomers of hydroxy analogues of branched chain amino acids as well as L-lactate share with the same H(+)-driven uptake system.
Friedrich
M
M
Zentralnstitut für Ernährung, Potsdam-Rehbrücke, Universität Zürich.
Murer
H
H
Berger
E G
EG
D000818:Animals; D001693:Biological Transport, Active; D046508:Culture Techniques; D006863:Hydrogen-Ion Concentration; D007421:Intestine, Small / Q000378:metabolism*; D007930:Leucine / Q000031:analogs & derivatives / Q000493:pharmacokinetics*; D008871:Microvilli / Q000378:metabolism; D011817:Rabbits
D007930:Leucine
Transport des Hydroxyanalogons von Leucin in Bürstensaum-Membranvesikel des Kaninchendünndarms.