T-lymphocyte-receptor repertoire of infiltrating T lymphocytes into NOD mouse pancreas.
This study analyzed T-lymphocyte-receptor V beta genes of infiltrating lymphocytes into the pancreases of 12- to 16-wk-old NOD mice with severe insulitis and 5-wk-old mice with mild insulitis by the quantitative polymerase chain reaction method. The V beta transcripts on infiltrating T lymphocytes into pancreases with severe insulitis in older NOD mice were diverse. In contrast, the V beta 11 gene transcript was predominantly expressed on T lymphocytes in the pancreas in younger NOD mice with mild insulitis, suggesting the possible role of V beta 11+ T lymphocytes in triggering insulitis in this species.
Maeda
T
T
Second Department of Internal Medicine, School of Medicine, Chiba University, Japan.
Sumida
T
T
Kurasawa
K
K
Tomioka
H
H
Itoh
I
I
Yoshida
S
S
Koike
T
T
D000818:Animals; D001483:Base Sequence; D015870:Gene Expression; D051379:Mice; D016688:Mice, Inbred NOD / Q000276:immunology*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010179:Pancreas / Q000276:immunology*; D010182:Pancreatic Diseases / Q000276:immunology; D016133:Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014158:Transcription, Genetic
D009838:Oligodeoxyribonucleotides; D011948:Receptors, Antigen, T-Cell