[The qualitative and quantitative analyses of protein with a novel fluorescence agent (EPQS) by fluorescent scanning on solid substrate].
We synthesized a new fluorescence agent, EPQS, which could be soluble in water and was stable in the analytical process. It was characteristic of good fluorescence parameters. Human serum albumin (HSA) was marked with EPQS and purified by the paper electrophoresis dialysis. We determined the fluorescent spectra and excitation spectra of the marked HSA, or EPQS on the cellulose acetate paper by RF-510 fluorescence spectrophotometer-thin-layer chromatoscanner. The former lambda ex = 370nm lambda em = 470nm; the latter lambda ex = 382nm lambda em = 476 nm. Then we touched different quantity of EPQS-HSA upon the cellulose acetate. The fluorescent scanning on the paper disclosed that the relationship between the quantity adopted and the fluorescence intensity is linear. These showed that the protein could be quickly determined qualitatively and quantitatively by this simple method, and EPQS was useful.
Kang
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S
Zou
Z
Z
Qiao
X
X
D002855:Chromatography, Thin Layer; D003720:Densitometry; D005456:Fluorescent Dyes; D006801:Humans; D007328:Insulin / Q000032:analysis*; D012709:Serum Albumin / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D007328:Insulin; D012709:Serum Albumin