Characteristics of the inhibition of interferon-alpha/beta production after crystalline nickel sulfide exposure.
Murine L-929 cells were treated with crystalline nickel sulfide (NiS), washed, and then exposed to polyriboinosinic-polyribocytidylic acid (Poly I:C) to induce interferon-alpha/beta (IFN-alpha/beta). Addition of crystalline NiS at different time points, from 24 hr prior to IFN induction (pretreatment) up to 12 hr after IFN induction, resulted in significant inhibition of IFN-alpha/beta production. In addition, 24-hr pretreatment of L-929 cells with crystalline NiS resulted in significantly decreased production of IFN-alpha/beta even 120 hr after addition of IFN inducer. IFN-alpha/beta at a known concentration was titered by means of a viral plaque reduction assay in crystalline NiS-treated L-929 cell cultures. Crystalline NiS did not show a significant inhibitory effect on the antiviral activity of IFN on L-929 cells as compared to controls. The results described here suggest that NiS has a rapid and a long-lasting inhibitory activity on the production of IFN-alpha/beta.
Jaramillo
A
A
Department of Microbiology and Immunology, School of Medicine, University of Louisville, Kentucky 40292.
Sonnenfeld
G
G
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D007370:Interferon Type I / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000276:immunology; D009532:Nickel / Q000633:toxicity*; D013997:Time Factors
D007370:Interferon Type I; D009532:Nickel; C017558:nickel sulfide