[Subcloning and sequencing of the Streptomyces griseus DNA fragment with promoter activity in E. coli].
A 410-bp DNA fragment of Streptomyces griseus No.45 with in vivo promoter activity in E. coli has been isolated by subcloning the cloning strain E. coli No.8-1. The result of sequencing shows that the promoter fragment contains 50.5% G + C bases. It has some repeat sequences. It shows good homology to the E. coli promoter consensus sequences in -10 and -35 regions. There are 18 bp between the two regions. It has two regions that are similar to SD sequence in E. coli and consensus sequence in SEP (Streptomyces-E.coli-type promoter) of Streptomyces lividans respectively. There is 1 Alu I site, 1 Cla I site, 2 Mbo I sites, 3 NlaIII sites and 1 PvuII site in the promoter fragment.
Chen
Y
Y
Institute of Microbiology, Academia Sinica Beijing.
Xue
Y G
YG
Dong
K N
KN
Liu
M H
MH
D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D013305:Streptomyces griseus / Q000235:genetics*
D004269:DNA, Bacterial