Evaluation of potential interactions involving trichothecene mycotoxins using the chick embryotoxicity bioassay.
The Chick Embryotoxicity Screening Test (CHEST) was used to examine possible interactions between different trichothecenes. Combinations of deoxynivalenol (DON), 15-acetyldeoxynivalenol (15-ADON) and HT-2 toxin were tested at various concentrations and the effects were determined based on percent mortality. The combined toxicity of any two trichothecenes was found to be additive. Discrepancies between observed and expected mortality values seldom differed by more than 10%, well within sampling error for the binomial distribution. Several limitations of the CHEST assay are discussed.
Rotter
B A
BA
Animal Research Centre, Agriculture Canada, Ottawa, Ontario.
Thompson
B K
BK
Prelusky
D B
DB
Trenholm
H L
HL
D000818:Animals; D001681:Biological Assay; D002642:Chick Embryo; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D014255:Trichothecenes / Q000633:toxicity*
D014255:Trichothecenes