Cross-neutralizing antibodies in rabbits immunized with HIV-1 gp160 purified from simian cells infected with a recombinant vaccinia virus.
A recombinant vaccinia virus in which the transcription of the human immunodeficiency virus type 1 (BRU isolate) env gene is driven by the 11K late vaccinia promoter yields about 10-fold higher amounts of gp160 env protein upon infection of monkey cells than does a recombinant in which gp160 is expressed using the 7.5K early-late promoter. The gp160 was purified from detergent lysates of infected cells by lentil lectin affinity chromatography followed by immunoaffinity chromatography, and was obtained in yields of 1-2 mg/10(9) cells of material estimated to be about 70% pure. Pairs of rabbits were immunized with purified gp160 using either one of five different adjuvants or an immunostimulating complex. In all cases a substantial humoral immune response was obtained after boosting, including an activity that neutralized the homologous (BRU) isolate of HIV-1. In some cases, this activity also neutralized two distantly related isolates, SF2 and MN.
Klaniecki
J
J
Bristol-Myers Squibb Pharmaceutical Research Institute Seattle, WA.
Dykers
T
T
Travis
B
B
Schmitt
R
R
Wain
M
M
Watson
A
A
Sridhar
P
P
McClure
J
J
Morein
B
B
Ulrich
J T
JT
D000818:Animals; D002460:Cell Line; D003429:Cross Reactions; D015686:Gene Products, env / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D015752:Genes, env; D015483:HIV Antibodies / Q000096:biosynthesis*; D019302:HIV Envelope Protein gp160; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D009500:Neutralization Tests; D011498:Protein Precursors / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000235:genetics
D015686:Gene Products, env; D015483:HIV Antibodies; D019302:HIV Envelope Protein gp160; D011498:Protein Precursors