title: rIL-1 alpha enhances adoptive transfer of resistance to Listeria monocytogenes infection.
abstract: We have previously demonstrated that administration of recombinant rIL-1 alpha enhances resistance against Listeria monocytogenes infection in mice. In this study we considered the possibility that this cytokine might also augment adoptive immunity conferred by the transfer of listeria-immune spleen cells. Concomitant administration of rIL-1 alpha with large numbers (2 x 10(7) or 10(8)) of listeria-immune spleen cells reduced the protection mediated by the transferred cells. Conversely, rIL-1 alpha co-administered with suboptimal numbers (1-5 x 10(6)) of immune splenocytes augmented anti-listeria resistance in an additive fashion. Although transfer of 10(6) listeria-immune spleen cells alone did not result in significant protection, when 10(6) immune cells were incubated with rIL-1 alpha prior to transfer they conferred significant protection to naive recipients. Time course experiments indicated that the greatest protection was achieved when listeria-immune spleen cells were pretreated with rIL-1 alpha for 2 h prior to adoptive transfer. The protection transferred by 10(6) rIL-1 alpha-pretreated immune spleen cells was not inhibited by TGF beta. This study is the first to use rIL-1 alpha to potentiate the adoptive transfer of resistance to an infectious agent by immune cells.
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, Madison, Wisconsin 53706.
authors.forename: M
authors.initials: M
authors.lastname: Haak-Frendscho
authors.forename: R S
authors.initials: RS
authors.lastname: Kurtz
authors.forename: C J
authors.initials: CJ
authors.lastname: Czuprynski
mesh_terms: D000818:Animals; D003131:Combined Modality Therapy; D007116:Immunization, Passive / Q000379:methods* / Q000662:veterinary; D007375:Interleukin-1 / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000276:immunology* / Q000472:pathogenicity; D008088:Listeriosis / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D016378:Tissue Transplantation / Q000502:physiology* / Q000662:veterinary; D016211:Transforming Growth Factor alpha / Q000494:pharmacology
chemical_list: D007375:Interleukin-1; D011994:Recombinant Proteins; D016211:Transforming Growth Factor alpha