1H-NMR structural determination of the N-linked carbohydrate chains on glycopeptides obtained from the bean lectin phytohemagglutinin.
Phytohemagglutinin, the lectin of the common bean Phaseolus vulgaris, is a N-linked glycoprotein with one high-mannose-type and one xylose-containing oligosaccharide side chain per polypeptide. The high-mannose-type glycan is attached to Asn12 and the complex-type glycan to Asn60 [Sturm, A. & Chrispeels, M. J. (1986) Plant Physiol. 81, 320-322]. The structures of the oligosaccharides were elucidated from two glycopeptides obtained from the lectin by Pronase digestion, affinity chromatography on concanavalin-A--Sepharose and gel-filtration chromatography on a column of BioGel P-4. The N-linked glycan structures were investigated by 500-MHz 1H-NMR spectroscopy and were established to be: [formula; see text]
Sturm
A
A
Friedrich Miescher-Institute, Basel, Switzerland.
Bergwerff
A A
AA
Vliegenthart
J F
JF
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D007887:Fabaceae / Q000737:chemistry; D006020:Glycopeptides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000032:analysis; D008969:Molecular Sequence Data; D010835:Phytohemagglutinins / Q000737:chemistry*; D037121:Plant Lectins; D010946:Plants, Medicinal; D014994:Xylose / Q000032:analysis
D006020:Glycopeptides; D010835:Phytohemagglutinins; D037121:Plant Lectins; D014994:Xylose; D008358:Mannose