title: LRV1 viral particles in Leishmania guyanensis contain double-stranded or single-stranded RNA.
abstract: The 32-nm-diameter spherical viral particles found in the cytoplasm of Leishmania guyanensis CUMC1-1A sediment at 130S and have a buoyant density of approximately 1.4 g/ml in cesium chloride gradients. These particles contain a 5.3-kb double-stranded RNA, while single-stranded RNA that corresponds to the viral positive strand is associated with less-dense particles. These results suggest a conservative and sequential mode of LRV1 viral RNA replication that is exemplified by the ScV L-A virus of yeast.
authors.affiliation: Seattle Biomedical Research Institute, Washington 98109-1651.
authors.forename: R
authors.initials: R
authors.lastname: Weeks
authors.forename: R F
authors.initials: RF
authors.lastname: Aline
authors.forename: P J
authors.initials: PJ
authors.lastname: Myler
authors.forename: K
authors.initials: K
authors.lastname: Stuart
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D003593:Cytoplasm / Q000382:microbiology; D007892:Leishmania braziliensis / Q000382:microbiology*; D015347:RNA Probes; D012328:RNA Viruses / Q000737:chemistry*; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*
chemical_list: D015347:RNA Probes; D012330:RNA, Double-Stranded; D012367:RNA, Viral