The effect of methylation inhibitors on citreamicin biosynthesis in Micromonospora citrea.
When the citreamicin-producing organism Micromonospora citrea NRRL 189351 was incubated in the presence of the methylation inhibitors sinefungin or aminopterin, biosynthesis of the zeta component was stimulated approximately 20 to 200-fold above the level normally produced. Inhibition of a second methylation reaction, which is superficially very similar to the first, was not detected. Other known methylation inhibitors failed to yield any change in the natural pattern of citreamicins produced. This approach is an excellent route for preparing citreamicin zeta, which can be used as a substrate for semi-synthesis or for further biosynthetic studies.
Pearce
C J
CJ
Medical Research Division, American Cyanamid Company, Lederle Laboratories, Pearl River, New York 10965.
Carter
G T
GT
Nietsche
J A
JA
Borders
D B
DB
Greenstein
M
M
Maiese
W M
WM
D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000630:Aminopterin / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D000935:Antifungal Agents / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D008745:Methylation / Q000187:drug effects; D008847:Micromonospora / Q000378:metabolism*; D010080:Oxazoles / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D010080:Oxazoles; C060116:citreamicin alpha; D000630:Aminopterin; D000241:Adenosine; C006235:sinefungin