[The effect of actinomycin D on lysosomal proteinase activity in murine macrophages and spleen during immune response].
Actinomycin D inhibited activation of lysosomal cathepsins A, B, C, D, H and L in peritoneal macrophages, caused by T-dependent antigen (sheep erythrocytes), of mice CBA X C57BL/6 strain. At the same time, the antibiotic stimulated activity of cathepsins A, C, D and H but decreased content of antibody-producing cells in spleen tissue. Inhibition of lysosomal proteinases in macrophages may be responsible for immunodepressive effect of actinomycin D.
Vasil'ev
A V
AV
Vorobeĭchik
T A
TA
Gutkin
D V
DV
Levitskaia
A B
AB
Tutel'ian
V A
VA
D000818:Animals; D000917:Antibody Formation; D002403:Cathepsins / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology*; D010450:Endopeptidases / Q000096:biosynthesis*; D004790:Enzyme Induction; D008247:Lysosomes / Q000201:enzymology*; D008264:Macrophages / Q000201:enzymology* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011480:Protease Inhibitors / Q000378:metabolism; D013154:Spleen / Q000201:enzymology* / Q000276:immunology
D011480:Protease Inhibitors; D003609:Dactinomycin; D002403:Cathepsins; D010450:Endopeptidases
Vliianie aktinomitsina D na aktivnost' lizosomal'nykh proteinaz v makrofagakh i selezenke mysheĭ pri immunnom otvete.