The direct cloning of the yeast genome using the gap-filling method and the complete physical mapping of Saccharomyces cerevisiae chromosome VI.
The ordered clone library of chromosome VI of Saccharomyces cerevisiae has been constructed by Olson et al. [Proc. Natl. Acad. Sci. USA 83 (1986) 7826-7830, and personal communication]. It is composed of four contiguous stretches from the chromosome, each of 40-70 kb. There remained three gaps of unknown length between these four contigs. We applied the 'gap-repair' method to clone these three gap regions directly from the yeast chromosome. All three gap regions, ranging from 7 to 22 kb, were successfully cloned without any structural changes. Together with these gap regions, a precise physical map of EcoRI and HindIII sites was constructed over the 230-kb fragment which covers most of chromosome VI except for two telomeres.
Iwasaki
T
T
Department of Genetics, Osaka University Medical School, Japan.
Shirahige
K
K
Yoshikawa
H
H
Ogasawara
N
N
D002874:Chromosome Mapping; D003001:Cloning, Molecular / Q000379:methods*; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D016681:Genome, Fungal; D008957:Models, Genetic; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII