title: Lysis and fractionation of Mycobacterium paratuberculosis and Escherichia coli by matrix solid-phase dispersion.
abstract: A novel method for the lysis and subsequent fractionation of bacterial constituents from Mycobacterium paratuberculosis strain 19698 (M. paratuberculosis) and Escherichia coli strain DH5 alpha utilizing the technique of matrix solid-phase dispersion (MSPD) is described. Bacteria were blended with octadecylsilyl (C18) derivatized silica to obtain cellular lysis. The blended material was used to prepare a column which was sequentially eluted with solvents of increasing polarity. Fractionation of cellular components was confirmed by analysis of the solvent extracts. The possible applicability of the MSPD technique as a general method for the lysis and fractionation of bacterial components is proposed.
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803.
authors.forename: M E
authors.initials: ME
authors.lastname: Hines
authors.forename: A R
authors.initials: AR
authors.lastname: Long
authors.forename: T G
authors.initials: TG
authors.lastname: Snider
authors.forename: S A
authors.initials: SA
authors.lastname: Barker
mesh_terms: D001431:Bacteriological Techniques; D001433:Bacteriolysis; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D008855:Microscopy, Electron, Scanning; D009161:Mycobacterium / Q000737:chemistry*; D010283:Paratuberculosis / Q000382:microbiology