Imaging and nanodissection of individual supercoiled plasmids by atomic force microscopy.
The atomic force microscope (AFM) was used to image supercoiled plasmid DNA deposited on a mica surface in either a hydrated or desiccated state. Hydrated plasmid was precisely cut by the scanning tip at a location determined by the instrument operator. Small pieces of DNA (100-150 nm in length) were excised and deposited adjacent to the dissected plasmid, demonstrating that it is possible to remove and manipulate genomic DNA fragments, unresolvable by light microscopy, from defined chromosomal locations by AFM.
Henderson
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Department of Zoology and Genetics, Iowa State University, Ames 50011.
D004278:DNA, Superhelical / Q000378:metabolism / Q000648:ultrastructure*; D008853:Microscopy / Q000379:methods*; D010957:Plasmids / Q000235:genetics
D004278:DNA, Superhelical