title: Chromatographic characterisation and biological activity of neuropeptides immunoreactive to antisera against Met5-enkephalin-Arg6-Phe7 (YGGFMRF) extracted from the blowfly Calliphora vomitoria (Diptera).
abstract: Neuropeptides identified with a radioimmunoassay specific for the C-terminus of Met5-enkephalin-Arg6-Phe7 (YGGFMRF) have been extracted from nervous tissues of the blowfly Calliphora vomitoria and also from whole flies. Chromatographic characterisation, based on criteria of molecular weight, charge and hydrophobicity, reveals a complex multiplicity of immunoreactive peptides. Variations in the amounts and types of peptides found within different nervous tissues is evidence that the cellular precursor processing is selective. Physiological studies on the isolated blowfly salivary gland show that synthetic YGGFMRF is a potent secretagogue with a maximal rate of fluid secretion induced at a concentration of between 10(-13) and 10(-12) M. The tetrapeptide comprising the last four residues of the C-terminus of YGGFMRF, Phe-Met-Arg-Phe, is equally potent. However, the carboxyamidated variants, YGGFMRF-NH2 and the molluscan cardioacceleratory peptide FMRF-NH2, as well as the opioid peptides Met5- and Leu5-enkephalin, have no activity. Partially purified YGGFMRF-immunoreactive peptides from the blowfly have ED50 values in the bioassay approximating to 0.3 thoracic ganglion, 2.1 hypocerebral ganglion and 3.0 brain equivalents.
authors.affiliation: School of Biological Sciences, Queen Mary and Westfield College, University of London, U.K.
authors.forename: H
authors.initials: H
authors.lastname: Duve
authors.forename: J C
authors.initials: JC
authors.lastname: Sewell
authors.forename: A G
authors.initials: AG
authors.lastname: Scott
authors.forename: A
authors.initials: A
authors.lastname: Thorpe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002845:Chromatography; D003429:Cross Reactions; D004175:Diptera / Q000502:physiology*; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000276:immunology / Q000378:metabolism; D005724:Ganglia / Q000737:chemistry; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000276:immunology / Q000378:metabolism*; D011863:Radioimmunoassay; D012469:Salivary Glands / Q000293:injuries / Q000502:physiology*
chemical_list: D009479:Neuropeptides; D004744:Enkephalin, Methionine; C027511:enkephalin-Met, Arg(6)-Phe(7)-