A comparative study of the effects of the diamine oxidase inhibitor aminoguanidine, with or without dietary restriction, on the nucleic acid and protein composition of cardiac and type I and type II skeletal muscles of the rat.
It has been proposed that the diamine oxidase inhibitor aminoguanidine may be a potential therapeutically important anabolic agent. An investigation was therefore made into the effects of aminoguanidine treatment with or without nutritional restriction, on cardiac and skeletal muscles containing mainly of either Type I (i.e. soleus) or Type II fibres (i.e. plantaris) or a mixture of Type I and II fibres (i.e. gastrocnemius). After 3 weeks, dietary restrictions reduced cardiac weight, protein, RNA and DNA contents by between 31 per cent and 36 per cent. Similar, but smaller, reductions were observed in the soleus (18-31 per cent), plantaris (22-34 per cent) and gastrocnemius (22-34 per cent). Aminoguanidine had no effect on the heart of the rats fed ad libitum, nor did it alter the response to dietary restriction. Treatment with aminoguanidine had no overt anabolic effect on skeletal muscle, but a reduction in DNA content was observed. It was concluded that cardiac protein and nucleic acid contents are more sensitive to dietary deprivation than either anaerobic or aerobic skeletal muscles. Furthermore, aminoguanidine does not appear to promote growth or reduce catabolism as previous studies have suggested.
Siddiq
T
T
Department of Cardiology, King's College School of Medicine and Dentistry, London, U.K.
Preedy
V R
VR
D006631:Amine Oxidase (Copper-Containing) / Q000187:drug effects; D000818:Animals; D004032:Diet / Q000009:adverse effects*; D006146:Guanidines / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects; D009124:Muscle Proteins / Q000187:drug effects / Q000378:metabolism; D009132:Muscles / Q000737:chemistry* / Q000187:drug effects; D009206:Myocardium / Q000737:chemistry*; D009696:Nucleic Acids / Q000187:drug effects / Q000378:metabolism; D051381:Rats
D006146:Guanidines; D009124:Muscle Proteins; D009696:Nucleic Acids; D006631:Amine Oxidase (Copper-Containing); C004479:pimagedine