[Transfer of transformation and conjugation plasmids from Escherichia coli to Bacillus subtilis and Azospirillum brasilense].
The conjugative transfer of RP4 plasmid from Escherichia coli to Azospirillum brasilense was detected after introduction and subsequent incubation of these microorganisms in soil. The plasmid transfer via transformation from Escherichia coli to Bacillus subtilis was observed in case both bacteria were growing together in sand containing sucrose solution. The possible reason for low frequency interspecies plasmid transformation under conditions close to natural habitats is poor survival of "domesticated" rather than wild type Bacillus subtilis strains and lack of competence state in this case.
Lukin
S A
SA
Kosovich
P V
PV
Prozorov
A A
AA
D016949:Azospirillum / Q000235:genetics*; D001412:Bacillus subtilis / Q000235:genetics*; D015169:Colony Count, Microbial; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D010957:Plasmids; D012988:Soil Microbiology; D014169:Transformation, Bacterial
Peredacha plazmid za schet transformatsii i kon''iugatsii ot Escherichia coli k Bacillus subtilis i Azospirillum brasilense.