Prostaglandin E2 receptor of rat submandibular salivary glands.
The binding characteristics of the PGE2 receptor were investigated in membrane preparations from these glands. Specific [3H]PGE2 binding was linear as a function of the membrane protein concentration and reached steady state by 40 min of incubation at 37 degrees C under neutral pH. Scatchard analysis of the binding data produced a curvilinear plot with a Kd of 0.18 nM and Bmax of 1.02 fmol/mg protein for the high-affinity binding sites, and a Kd of 181 nM and Bmax of 5.72 pmol/mg protein for the low-affinity binding sites. A competitive displacement study indicated that the receptor was specific for prostaglandins of the E series. The study is the first to demonstrate the presence of the PGE2 receptor in rat submandibular gland and to provide its biochemical features.
Wu-Wang
C Y
CY
Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
Wang
S L
SL
Yao
P
P
Slomiany
A
A
Slomiany
B L
BL
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D005069:Evaluation Studies as Topic; D006863:Hydrogen-Ion Concentration; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry*; D011458:Prostaglandins E; D051381:Rats; D011919:Rats, Inbred Strains; D011982:Receptors, Prostaglandin / Q000737:chemistry*; D012469:Salivary Glands / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
D008565:Membrane Proteins; D011458:Prostaglandins E; D011982:Receptors, Prostaglandin