Release of corticotrophin-releasing factor-41, arginine vasopressin and oxytocin from rat fetal hypothalamic cells in culture: response to activation of intracellular second messengers and to corticosteroids.
The effects of the activation of protein kinase A (PKA), protein kinase C (PKC) and corticosteroids were investigated on the release of corticotrophin-releasing factor-41 (CRF), arginine vasopressin (AVP) and oxytocin from rat fetal hypothalamic cells in culture. Both forskolin and PMA (phorbol 12-myristate 13-acetate) increased CRF, AVP and oxytocin release, while dexamethasone and aldosterone only reduced basal secretion of CRF. Both steroids also inhibited forskolin-induced CRF, AVP and oxytocin responses to PMA. These data provide direct evidence for a role for both PKC- and PKA-mediated mechanisms in the regulation of CRF, AVP and oxytocin release and for differential effects of both glucocorticoids and mineralocorticoids on PKA- and PKC-stimulated responses.
Hu
S B
SB
Neuroendocrinology Unit, Charing Cross and Westminster Medical School, Charing Cross Hospital, London.
Tannahill
L A
LA
Biswas
S
S
Lightman
S L
SL
D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D000450:Aldosterone / Q000494:pharmacology; D000818:Animals; D001127:Arginine Vasopressin / Q000096:biosynthesis*; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D003346:Corticotropin-Releasing Hormone / Q000096:biosynthesis*; D003907:Dexamethasone / Q000494:pharmacology; D004789:Enzyme Activation / Q000502:physiology; D005260:Female; D007031:Hypothalamus / Q000166:cytology / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D010121:Oxytocin / Q000096:biosynthesis*; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015290:Second Messenger Systems / Q000187:drug effects* / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000305:Adrenal Cortex Hormones; D001127:Arginine Vasopressin; D005576:Colforsin; D000450:Aldosterone; D010121:Oxytocin; D003907:Dexamethasone; D003346:Corticotropin-Releasing Hormone; D011494:Protein Kinases; D013755:Tetradecanoylphorbol Acetate