Quantitative microculture system with standardized inocula for strain typing, susceptibility testing, and other physiologic measurements with Candida albicans and other yeasts.
A method for physiologic testing of Candida albicans and other yeasts is described in which inocula with reproducible cell concentrations are prepared from cultures in a glucose-limited medium and growth turbidities of test cultures in microplates are expressed quantitatively as a percentage of control growth. The method gave good within-plate and run-to-run reproducibilities and was applicable both to strain phenotype discrimination in C. albicans and to susceptibility tests of yeasts with azole antifungal agents.
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Department of Bacteriology and Mycology, Janssen Research Foundation, Beerse, Belgium.
D000362:Agar; D002176:Candida albicans / Q000145:classification* / Q000187:drug effects / Q000254:growth & development; D003470:Culture Media; D008826:Microbial Sensitivity Tests / Q000592:standards*; D016533:Mycological Typing Techniques / Q000592:standards*; D009391:Nephelometry and Turbidimetry; D012015:Reference Standards; D013535:Suspensions; D014867:Water; D015003:Yeasts / Q000145:classification* / Q000187:drug effects / Q000254:growth & development
D003470:Culture Media; D013535:Suspensions; D014867:Water; D000362:Agar