title: Brain ouabain and central effects of dietary sodium in spontaneously hypertensive rats.
abstract: High sodium intake (HNa) increases brain ouabainlike activity (OLA) in rats. In spontaneously hypertensive rats (SHR), HNa exaggerates development of hypertension and pressor and sympathoexcitatory responses to stress. To investigate whether dietary sodium-induced changes in brain OLA play a functional role, responses of mean arterial pressure (MAP), heart rate (HR), and renal sympathetic nerve activity (RSNA) to intracerebroventricular ouabain and to mental stress and intracerebroventricular alpha 2-adrenoceptor agonist guanabenz alone or preceded by intracerebroventricular ouabain were recorded in conscious SHR and Wistar-Kyoto (WKY) rats maintained from 4 to 8 weeks of age on different sodium diets: 1) low sodium intake (LNa, 17 mumol), 2) normal sodium intake (NNa, 101 mumol), and 3) HNa (1,370 mumol). SHR on NNa showed significantly higher MAP and RSNA compared with WKY rats on NNa. HNa or LNa significantly increased or decreased MAP but had no effects on resting RSNA in SHR and had no effects on resting MAP and RSNA in WKY rats. Intracerebroventricular ouabain induced dose-dependent increases in MAP, RSNA, and HR. In both SHR and WKY rats, LNa significantly enhanced these responses. In contrast, HNa significantly attenuated these responses only in SHR. Air stress increased and intracerebroventricular guanabenz decreased MAP, HR, and RSNA. The magnitudes of increases and decreases were significantly larger in SHR than in WKY rats. In WKY rats, dietary sodium did not change these responses. In contrast, in SHR, HNa significantly enhanced MAP, HR, and RSNA responses to air stress or intracerebroventricular guanabenz.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Hypertension Unit, University of Ottawa Heart Institute, Ontario, Canada.
authors.forename: B S
authors.initials: BS
authors.lastname: Huang
authors.forename: F H
authors.initials: FH
authors.lastname: Leenen
mesh_terms: D000818:Animals; D001794:Blood Pressure; D001921:Brain / Q000187:drug effects / Q000502:physiology*; D004032:Diet; D006143:Guanabenz / Q000494:pharmacology; D006339:Heart Rate; D006973:Hypertension / Q000378:metabolism*; D007276:Injections, Intraventricular; D007668:Kidney / Q000294:innervation; D008297:Male; D010042:Ouabain / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012146:Rest; D012964:Sodium / Q000008:administration & dosage* / Q000494:pharmacology; D013315:Stress, Psychological / Q000503:physiopathology; D013564:Sympathetic Nervous System / Q000502:physiology
chemical_list: D010042:Ouabain; D012964:Sodium; D006143:Guanabenz