Distinctive infrared spectral features in liver tumor tissues of mice: evidence of structural modifications at the molecular level.
Mice were treated with griseofulvin (GF) containing diet or control diet for 12 months. The livers from mice fed griseofulvin showed large tumors that were excised and used for analysis. The infrared spectra from control liver tissue and tumor tissue from GF livers were measured and compared as a function of pressure up to 27 kbar. Many changes in the infrared spectral features of the tumor tissue were observed. Results showed that neoplasm formation involved structural modifications of nucleic acids, lipids, carbohydrates, and proteins in the liver cells, which were detected from the abnormal vibrations of the functional groups in these biomolecules. The amount of glycogen was dramatically decreased in the tumor tissue compared to the control tissue. Important changes in the strength of hydrogen-bondings in the phosphodiester backbone of the nucleic acids and in the C-O groups of tissue proteins and carbohydrates were observed. Stronger interchain interactions and thus close interchain packing among the lipids in the GF liver were evident. These results showed very close similarities with those obtained with other types of tumors such as human colon cancer, suggesting that a common pattern of molecular changes has been identified in neoplastic transformation.
Wong
P T
PT
Steacie Institute for Molecular Science, National Research Council of Canada, Ottawa, Ontario.
Cadrin
M
M
French
S W
SW
D000577:Amides / Q000737:chemistry; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000737:chemistry*; D006003:Glycogen / Q000737:chemistry; D006118:Griseofulvin / Q000633:toxicity; D006860:Hydrogen Bonding; D008114:Liver Neoplasms, Experimental / Q000737:chemistry*; D051379:Mice; D008809:Mice, Inbred C3H; D010710:Phosphates / Q000737:chemistry; D011312:Pressure; D013055:Spectrophotometry, Infrared; D014280:Triglycerides / Q000737:chemistry
D000577:Amides; D010710:Phosphates; D014280:Triglycerides; D006118:Griseofulvin; D006003:Glycogen