title: Differential requirement of ATP and extra-ribosomal proteins for ribosome inactivation by eight RNA N-glycosidases.
abstract: The requirement of ATP and extra-ribosomal proteins for the inactivation of ribosomes by eight plant RNA N-glycosidases [ribosome-inactivating proteins (RIPs)] was investigated. Tritin, pokeweed antiviral protein and barley RIP depend, as gelonin [Sperti, S., Brigotti, M., Zamboni, M., Carnicelli, D. and Montanaro, L. (1991) Biochem. J., 277, 281-284], on the presence of ATP and extra-ribosomal proteins for full inactivation of ribosomes, while bryodin, lychnin, momordin, momorcochin and saporin inactivate isolated Artemia salina ribosomes suspended in buffer saline.
authors.affiliation: Dipartimento di Patologia sperimentale dell'Università di Bologna, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Carnicelli
authors.forename: M
authors.initials: M
authors.lastname: Brigotti
authors.forename: L
authors.initials: L
authors.lastname: Montanaro
authors.forename: S
authors.initials: S
authors.lastname: Sperti
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D006026:Glycoside Hydrolases / Q000494:pharmacology*; D007700:Kinetics; D010940:Plant Proteins / Q000494:pharmacology*; D010944:Plants / Q000201:enzymology*; D011072:Poly U / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects*; D012339:RNA, Ribosomal, 28S / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D012339:RNA, Ribosomal, 28S; D011072:Poly U; D000255:Adenosine Triphosphate; D006026:Glycoside Hydrolases