One-step transformation of yeast in stationary phase.
A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45 degrees C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5 x 10(8) cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 10(4) transformants/microgram plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.
Chen
D C
DC
Graduate Institute of Botany, National Taiwan University, Taipei, Republic of China.
Yang
B C
BC
Kuo
T T
TT
D004271:DNA, Fungal / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics; D014170:Transformation, Genetic; D015003:Yeasts / Q000235:genetics* / Q000254:growth & development
D004271:DNA, Fungal