title: Suckling stimulates the expression of vasoactive intestinal polypeptide gene in rats.
abstract: The effect of suckling on vasoactive intestinal polypeptide (VIP) gene expression in the hypothalami was studied during the postpartum period in rats. Female rats were divided into two groups immediately after delivery. In one group, a mother was housed with 8 pups, and in the other, without any pups. The former group was named S(+) and the latter S(-). On days 0, 3, 6, 9 and 12 after delivery, the mothers were killed by decapitation. Hypothalamic VIP mRNA was measured by RNA dot hybridization. Although the VIP mRNA level showed no significant change after delivery in the S(-) group, VIP mRNA in the S(+) group on days 6, 9 and 12 increased to 1.6, 3.5 and 2.1 times higher than the level observed on day 0, respectively. These results suggest that suckling induces the synthesis of VIP after 6 postpartum days.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Nagoya University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ishihara
authors.forename: H
authors.initials: H
authors.lastname: Seo
authors.forename: N
authors.initials: N
authors.lastname: Suganuma
authors.forename: H
authors.initials: H
authors.lastname: Oguri
authors.forename: K
authors.initials: K
authors.lastname: Chihara
authors.forename: N
authors.initials: N
authors.lastname: Matsui
authors.forename: Y
authors.initials: Y
authors.lastname: Tomoda
mesh_terms: D000199:Actins / Q000096:biosynthesis; D000818:Animals; D015152:Blotting, Northern; D003720:Densitometry; D005260:Female; D015870:Gene Expression; D007031:Hypothalamus / Q000378:metabolism; D007774:Lactation / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010902:Pituitary Gland / Q000378:metabolism; D049590:Postpartum Period / Q000378:metabolism; D011388:Prolactin / Q000097:blood; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014660:Vasoactive Intestinal Peptide / Q000096:biosynthesis*
chemical_list: D000199:Actins; D012333:RNA, Messenger; D014660:Vasoactive Intestinal Peptide; D011388:Prolactin