Determination by latex immunoassay of protein 1 in normal and pathological urine.
A sensitive latex particle assay has been developed to study the occurrence of protein 1 in human urine. The coefficients of variation (CVs) of the method which is fully automated vary between 3 and 11.5%. The assayable concentration range is 0.3 to 40 micrograms/l. Protein 1 is clearly a sex-dependent protein. In contrast to urinary retinol-binding protein (RBP) which shows no variation with age or sex, protein 1 is excreted in greater amounts in males from the puberty. In adults, the mean concentration of protein 1 in urine of men is approximately 5 times that of women. In the urine from both sexes, protein 1 occurs as a single component with a Mr around 21,000 and an pI of about 4.8. Protein 1 is correlated with RBP in the urine from female or male patients with impaired proximal tubular function, which suggests that it is handled by the kidney in a similar manner as RBP. Diabetics, however, show elevations of urinary protein 1 which do not correlate with the RBP excretion but with the albuminuria. A competition between albumin and protein 1 for renal tubular uptake might explain this paradoxical behaviour of protein 1 in the course of diabetic nephropathy.
Bernard
A
A
Unité de Toxicologie Industrielle et de Médecine du Travail, Université Catholique de Louvain, Brussels, Belgium.
Lauwerys
R
R
Noel
A
A
Vandeleene
B
B
Lambert
A
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D003920:Diabetes Mellitus / Q000652:urine; D005260:Female; D006801:Humans; D007118:Immunoassay; D007840:Latex; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D012177:Retinol-Binding Proteins / Q000652:urine; D014598:Uteroglobin; D001613:beta 2-Microglobulin / Q000652:urine
D007840:Latex; D011506:Proteins; D012177:Retinol-Binding Proteins; C556389:SCGB1A1 protein, human; D001613:beta 2-Microglobulin; D014598:Uteroglobin