title: One-step transformation of yeast in stationary phase.
abstract: A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45 degrees C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5 x 10(8) cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 10(4) transformants/microgram plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.
authors.affiliation: Graduate Institute of Botany, National Taiwan University, Taipei, Republic of China.
authors.forename: D C
authors.initials: DC
authors.lastname: Chen
authors.forename: B C
authors.initials: BC
authors.lastname: Yang
authors.forename: T T
authors.initials: TT
authors.lastname: Kuo
mesh_terms: D004271:DNA, Fungal / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics; D014170:Transformation, Genetic; D015003:Yeasts / Q000235:genetics* / Q000254:growth & development
chemical_list: D004271:DNA, Fungal