Plasma volume expansion in humans after a single intense exercise protocol.
We used intense intermittent exercise to produce a 10% expansion of plasma volume (PV) within 24 h and tested the hypothesis that PV expansion is associated with an increase in plasma albumin content. The protocol consisted of eight 4-min bouts of exercise at 85% maximal O2 uptake with 5-min recovery periods between bouts. PV, plasma concentrations of albumin and total protein (TP), and plasma osmolality were measured before and during exercise and at 1, 2, and 24 h of recovery from exercise. During exercise, PV decreased by 15%, while plasma TP and albumin content remained at control levels. At 1 h of recovery, plasma albumin content was elevated by 0.17 +/- 0.04 g/kg body wt, accounting for the entire increase in plasma TP content. PV returned to control level at 1 h of recovery without fluid intake by the subjects, despite a 820 +/- 120-g reduction in body weight. At 2 h of recovery, plasma TP content remained significantly elevated, and plasma TP and albumin concentration were significantly elevated. At 24 h of recovery, PV was expanded by 4.5 +/- 0.7 ml/kg body wt (10 +/- 1%), estimated from hematocrit and hemoglobin changes, and by 3.8 +/- 1.3 ml/kg body wt (8 +/- 3%), measured by Evans blue dye dilution. Plasma albumin content was increased by 0.19 +/- 0.05 g/kg body wt at 24 h of recovery. If 1 g of albumin holds 18 ml of water, this increase in plasma albumin content can account for a 3.4-ml/kg body wt expansion of the PV. No significant changes in plasma osmolality occurred during recovery, but total plasma osmotic content increased in proportion to PV.(ABSTRACT TRUNCATED AT 250 WORDS)
Gillen
C M
CM
John B. Pierce Laboratory, Yale University School of Medicine, New Haven, Connecticut 06519.
Lee
R
R
Mack
G W
GW
Tomaselli
C M
CM
Nishiyasu
T
T
Nadel
E R
ER
D000293:Adolescent; D000328:Adult; D001798:Blood Proteins / Q000378:metabolism; D001810:Blood Volume / Q000502:physiology; D015444:Exercise / Q000502:physiology*; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D008297:Male; D009994:Osmolar Concentration; D010953:Plasma Volume / Q000502:physiology*; D012709:Serum Albumin / Q000378:metabolism; D013997:Time Factors
D001798:Blood Proteins; D006454:Hemoglobins; D012709:Serum Albumin