[The determination of protein content of milk, cheese, and meat with the use of the biuret reaction].
A review is given of the different composition of biuret test solutions and experiments with the estimation of protein content of milk, cheese and meat by means of the biuret method are reported. The pure protein and the casein content of skimmed milk and the protein content of whole milk could be determined with biuret test solutions containing potassium hydroxide and a detergent. The use of the biuret method is advantageous in the case of cheese and meat, which are dissolved in potash lye or alkaline detergent solutions. Disturbances of the biuret method caused by lactose, fat or turbidity were eliminated after addition of hydrogen peroxide, by means of extraction or of additional measurements with a copper-free, zinc-containing biuret reagent. Deviations of the color intensity of biuret complexes were compensated by inclusion of standards in the measurement series.
Reichardt
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W
Thuringer Landwirtshaftliche Untersuchungs- Forschungsansalt (i. A.), Fachbereich Tierzucht/Tierhaltung, Clausberg, Bundesrepublik Deutschland.
Eckert
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W
D000818:Animals; D001738:Biuret Reaction; D002417:Cattle; D002611:Cheese / Q000032:analysis*; D004044:Dietary Proteins / Q000032:analysis*; D006861:Hydrogen Peroxide; D006878:Hydroxides; D007202:Indicators and Reagents; D008460:Meat / Q000032:analysis*; D008894:Milk Proteins / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D011188:Potassium; D017680:Potassium Compounds
D004044:Dietary Proteins; D006878:Hydroxides; D007202:Indicators and Reagents; D008894:Milk Proteins; D017680:Potassium Compounds; D006861:Hydrogen Peroxide; D011188:Potassium; C029943:potassium hydroxide
Zur Bestimmung des Proteingehaltes von Milch, Kase and Fleisch mit Hilfe der Biuret-Reaktion.