title: Recombinant porcine somatotropin: an immunotoxicology study.
abstract: The present study was designed to determine the effect of recombinant porcine somatotropin (rpST; 5, 15, or 25 mg.pig-1.d-1 for 57 d) on a variety of immune function variables. We observed no significant effect of rpST treatment on the gross pathology of the pigs, histopathology of the immune system organs, total and differential white blood cell counts, lymphocyte blastogenic response to mitogens, or the neutrophil functions of chemotaxis, ingestion, reduction of cytochrome C, iodination, and antibody-dependent cell-mediated cytotoxicity. Those variables that were significantly affected by rpST treatment include a decreased hemoglobin and packed red blood cell volume (at some time points for all three rpST dosages), a decrease in plasma protein level at the 25-mg dose, a small increase in neutrophil random migration (at all three rpST dosages), and a decrease in IgG antibody response to tetanus toxoid at 15 d after immunization (but not at d 8, 22, or 29). Additionally, rpST treatment was associated with a decreased rate of BW gain (at 15-mg dose), increased liver and kidney weights (at all three dosage levels), and an increased incidence of renal tubular cytoplasmic vacuolation of minor severity. There were no observed differences in the overall health of the pigs due to rpST treatment, based on clinical observations as well as determination of antibody titer to, and isolation of, common swine pathogens. Therefore, there was no evidence that the observed influence of rpST treatment on immune function would be clinically relevant.
authors.affiliation: Dept. of Microbiol., Immunol., and Preventive Med., Iowa State University, Ames 50011.
authors.forename: B L
authors.initials: BL
authors.lastname: Goff
authors.forename: K P
authors.initials: KP
authors.lastname: Flaming
authors.forename: D E
authors.initials: DE
authors.lastname: Frank
authors.forename: J A
authors.initials: JA
authors.lastname: Roth
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D001798:Blood Proteins / Q000032:analysis; D001835:Body Weight / Q000187:drug effects; D016950:Bordetella bronchiseptica / Q000302:isolation & purification; D005260:Female; D013006:Growth Hormone / Q000633:toxicity*; D007107:Immune System / Q000187:drug effects*; D007109:Immunity / Q000187:drug effects*; D007111:Immunity, Cellular / Q000187:drug effects; D007958:Leukocyte Count / Q000662:veterinary; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D009929:Organ Size / Q000187:drug effects; D016979:Pasteurella multocida / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000633:toxicity; D013552:Swine / Q000276:immunology*
chemical_list: D001798:Blood Proteins; D011994:Recombinant Proteins; D013006:Growth Hormone