The use of irradiation and fusion gene transfer (IFGT) hybrids to isolate DNA clones from human chromosome region 9q33-q34.
We have generated somatic cell hybrids containing fragments of human chromosome arm 9q by an irradiation and fusion technique. No selection for human material was imposed, but of 23 clones analyzed most contained human DNA sequences and many contained multiple fragments of the human chromosome arm. A hybrid that appears to contain only two small fragments of human DNA from the regions of q33 and q34 has been used as a source from which to clone probes specific to those areas of the chromosome.
Florian
F
F
Department of Genetics and Biometry, University College London, U.K.
Hornigold
N
N
Griffin
D K
DK
Delhanty
J D
JD
Sefton
L
L
Abbott
C
C
Jones
C
C
Goodfellow
P N
PN
Wolfe
J
J
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular / Q000379:methods*; D006224:Cricetinae; D015342:DNA Probes / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014162:Transfection
D015342:DNA Probes; D005819:Genetic Markers; D011993:Recombinant Fusion Proteins