title: [Design of a species-specific DNA probe based on the pFra plasmid of the plague pathogen].
abstract: The recombinant plasmid pBS1 carrying a 2 kb SalGI fragment of Yersinia pestis pFra plasmid was constructed by insertion of the fragment into a vector plasmid pBR327. SalGI-BspRI 400 bp subfragment was recloned into a pBR322 vector plasmid. Open reading frame was found in the fragment by DNA sequencing technique. The subfragment designated F1-probe permits one to identify specifically the Yersinia pestis strains harbouring pFra plasmid, thus, differing them from closely related Yersiniea and other representatives of Enterobacteriaceae family.
authors.forename: O G
authors.initials: OG
authors.lastname: Shishkina
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Popov
authors.forename: O A
authors.initials: OA
authors.lastname: Kirillina
authors.forename: S V
authors.initials: SV
authors.lastname: Mashko
authors.forename: M I
authors.initials: MI
authors.lastname: Lebedeva
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015342:DNA Probes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D015183:Restriction Mapping; D013045:Species Specificity; D015010:Yersinia pestis / Q000235:genetics*
chemical_list: D015342:DNA Probes
vernacular_title: Konstruirovanie vidospetsificheskogo DNK-zonda na osnove plazmidy pFra chumnogo mikroba.