Monoclonal antibodies against soman: characterization of soman stereoisomers.
Hybridomas were produced which expressed monoclonal anti-soman antibodies as determined by microtiter enzyme-linked-antibody immunoassay (EIA). Each of these antibodies was titrated using a competitive inhibition enzyme immunoassay (CIEIA) with a variety of test ligands. The ligands used included soman (a racemic mixture), sarin, tabun, and each of the four stereoisomers of soman (C+ P+, C+ P-, C-P+ and C-P-). In all cases the antibodies tested exhibited IC50 values of 10(-4)-5 x 10(-6) M for soman. When sarin or tabun was used as a ligand, the antibodies exhibited no cross reactivity. All of the antibodies cross reacted with the four soman stereoisomers. A second group of hybridomas were produced which expressed monoclonal antibodies against CsPs-soman. These antibodies were used to make preliminary absolute chiral assignments to the four soman stereoisomers.
Lenz
D E
DE
United States Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD 21010-5425.
Yourick
J J
JJ
Dawson
J S
JS
Scott
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D006241:Haptens / Q000737:chemistry; D051379:Mice; D012999:Soman / Q000737:chemistry / Q000276:immunology*; D013237:Stereoisomerism
D000911:Antibodies, Monoclonal; D006241:Haptens; D012999:Soman