[Nucleic acid synthesis and spatial organization of DNA in the presence of heparin and the quaternary ammonium salt of the conidin oligomer-25].
A comparative study of polyconidin effects in the replicative and transcription activity of hepatocyte DNA in intact animals, and on peculiarities of spatial organization DNA in the DNA complex with oligomer-25 conidin was carried out. It was shown that polyconidin binding to DNA results in "cross-linking". This process is accompanied by formation of liquid-crystalline dispersion without abnormal optical activity. Liquid crystals possess high density packing of DNA molecules complexed with quaternary ammonium salt of oligomer-25 conidin. The addition of heparin to liquid-crystalline dispersions or phases, destroys the structure of the DNA complex with quaternary ammonium salt of oligomer-25 conidin. As a result "free" DNA molecules appear, they form the cholesteric liquid-crystalline phase. An increase in transcription activity and synchronization of DNA synthesis in hepatocyte was demonstrated. A correlation between chromatin modification and biological activity of chromatin after formation of the DNA--oligomer-25 conidin complex is proposed.
Khatsernova
B Ia
BIa
Silaeva
S A
SA
Golenchenko
V A
VA
Skuridin
S G
SG
Zaĭtsev
I I
II
Rozkin
M Ia
MIa
Evdokimov
Iu M
IuM
Rybin
V I
VI
Grigorova
L V
LV
Efimov
V S
VS
D000818:Animals; D002942:Circular Dichroism; D003432:Cross-Linking Reagents; D004247:DNA / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics; D006493:Heparin / Q000494:pharmacology*; D006494:Heparin Antagonists; D008099:Liver / Q000378:metabolism; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000096:biosynthesis*; D011108:Polymers / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D051381:Rats; D013936:Thymidine / Q000378:metabolism; D014158:Transcription, Genetic
D003432:Cross-Linking Reagents; D006494:Heparin Antagonists; D009696:Nucleic Acids; D011108:Polymers; D000644:Quaternary Ammonium Compounds; C039164:conidine; D006493:Heparin; D004247:DNA; D013936:Thymidine
Sintez nukleinovykh kislot i prostranstvennaia organizatsiiaDNK v prisutstvii geparina i chetvertichnoĭ ammoniĭnoĭ soli oligomera-25 konidina.