The effect of HP-1 and related neutrophil granule peptides on DNA synthesis in HL60 cells.
The recently isolated cysteine- and arginine-rich peptides of the neutrophil primary granule and the murine intestinal Paneth cell, named defensins or corticostatins, have a number of distinct biological properties. We report the effects of three of these peptides HP-1, HP-1-56 and HP-4 on the incorporation of [3H]thymidine into the DNA of the leukemic cell line HL60. HP-1 and HP-1-56, but not HP-4, inhibited DNA synthesis at 1-50 nM without causing cell death. At higher concentrations the effect was reversed, resulting in a small, but statistically significant increase in DNA synthesis at 1 microM. In contrast HP-4 had no effect on HL60 cells at nanomolar concentrations but was strongly cytotoxic at micromolar concentrations.
Bateman
A
A
Endocrine Laboratory, Royal Victoria Hospital/McGill University, Montreal, Quebec, Canada.
Singh
A
A
Congote
L F
LF
Solomon
S
S
D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D002461:Cell Line, Transformed; D003594:Cytoplasmic Granules / Q000737:chemistry; D004247:DNA / Q000097:blood; D023082:Defensins; D004305:Dose-Response Relationship, Drug; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D010455:Peptides; D016415:Sequence Alignment; D013234:Stem Cells / Q000187:drug effects*; D023084:alpha-Defensins
D001798:Blood Proteins; D023082:Defensins; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D023084:alpha-Defensins; C406413:human neutrophil peptide 1; C406428:human neutrophil peptide 4; C054598:corticostatin; D004247:DNA