Antibodies to human leucocyte antigens indicate subpopulations of microglia in human retina.
Monoclonal antibodies to human leucocyte antigens, including anti-CD45 and anti-CD68, have been used to describe microglia in flatmounts of normal adult human retina for the first time. Anti-CD45 (the leucocyte common antigen) intensely labeled large numbers of cells in a regular distribution across the retina; anti-CD68 and anti-macrophage antibodies labeled fewer cells with distinctive morphologies, suggesting the presence of subpopulations of microglia in the human retina expressing leucocyte antigens.
Penfold
P L
PL
Department of Ophthalmology, University of Sydney, Australia.
Madigan
M C
MC
Provis
J M
JM
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D015703:Antigens, CD / Q000378:metabolism; D002452:Cell Count; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006680:HLA Antigens / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D008264:Macrophages / Q000378:metabolism; D008875:Middle Aged; D009457:Neuroglia / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*; D013834:Thiamine Pyrophosphatase / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D005904:Glial Fibrillary Acidic Protein; D006680:HLA Antigens; D013834:Thiamine Pyrophosphatase