title: [The effect of various culture parameters on the yield of restriction endonuclease Sfa NI].
abstract: A simple technique is proposed for testing the restriction endonuclease Sfa NI activity in lysates of Streptococcus faecalis cells. The technique was used to study the effect of inorganic phosphate and the growth phase on the enzyme yield. Conditions were chosen that provide a high yield of the Sfa NI activity and a significantly reduced level of nucleases in the cells.
authors.forename: O A
authors.initials: OA
authors.lastname: Zhilkina
authors.forename: V E
authors.initials: VE
authors.lastname: Repin
authors.forename: S Kh
authors.initials: SKh
authors.lastname: Degtiarev
mesh_terms: D003470:Culture Media; D015252:Deoxyribonucleases, Type II Site-Specific / Q000096:biosynthesis* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D013293:Enterococcus faecalis / Q000201:enzymology* / Q000254:growth & development; D010710:Phosphates / Q000737:chemistry
chemical_list: D003470:Culture Media; D010710:Phosphates; D015252:Deoxyribonucleases, Type II Site-Specific; C065993:endodeoxyribonuclease SfaNI
vernacular_title: Vliianie nekotorykh parametrov kul'tivirovaniia na vykhod éndonukleazy restriktsii Sfa NI.