[Subcloning and study of the GTP-cyclohydrolase gene of Bacillus subtilis].
Bacillus subtilis GTP-cyclohydrolase gene and its deletion derivatives were subcloned in Escherichia coli cells. The position of the gene within the riboflavine operon was defined. The deletion of the 14 kDa fragment from the N-end of GTP-cyclohydrolase gene did not affect the enzyme activity.
Boretskiĭ
Iu R
IuR
Drobinskaia
I E
IE
Batchikova
N V
NV
Bidnenko
V E
VE
Rabinovich
P M
PM
D001412:Bacillus subtilis / Q000201:enzymology*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004586:Electrophoresis; D006136:GTP Cyclohydrolase / Q000235:genetics*; D008274:Magnesium / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D009876:Operon; D010957:Plasmids; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D012256:Riboflavin / Q000235:genetics
D004269:DNA, Bacterial; D008345:Manganese; D006136:GTP Cyclohydrolase; D008274:Magnesium; D012256:Riboflavin
Subklonirovanie i issledovanie gena GTP-tsiklogidrolazy Bacillus subtilis.