Ca2+ as a messenger of dorsal--ventral polarity formation in frog (Rana temporaria) eggs.
Three methods of Ca2+ administration were used to influence the location of the grey crescent and the dorsal lip of blastopore in R temporaria eggs, ie a Ca2+ microinjection into the subcortical cytoplasm, egg pricking in high Ca2+ solutions and Ca2+ ionophore A23187 microinjection and application. The treatments all induced grey crescent and dorsal lip of blastopore formation near the Ca2+ administration site. Inositol trisphosphate injections gave similar results. Colchicine injections into the eggs inhibited the appearance of both natural and Ca(2+)-induced grey crescents.
Bozhkova
V P
VP
Institute of Information Transmission Problems, USSR Academy of Sciences, Moscow.
Voronov
D A
DA
D000818:Animals; D000001:Calcimycin / Q000008:administration & dosage / Q000494:pharmacology; D002118:Calcium / Q000008:administration & dosage / Q000494:pharmacology*; D016764:Cell Polarity / Q000187:drug effects*; D003078:Colchicine / Q000008:administration & dosage / Q000494:pharmacology; D005775:Gastrula / Q000502:physiology; D015544:Inositol 1,4,5-Trisphosphate / Q000008:administration & dosage / Q000494:pharmacology; D008845:Microinjections; D010063:Ovum / Q000187:drug effects / Q000502:physiology*; D011896:Rana temporaria / Q000502:physiology*; D012965:Sodium Chloride / Q000008:administration & dosage / Q000494:pharmacology
D000001:Calcimycin; D012965:Sodium Chloride; D015544:Inositol 1,4,5-Trisphosphate; D003078:Colchicine; D002118:Calcium