title: Comparative effects of adrenaline and noradrenaline on the synthesis and secretion of soluble protein by isolated hepatocytes.
abstract: 1. Adrenaline (A) supplementation of the incubation medium of monolayer cultures of hepatocytes at 0.1, 0.2, 0.3, 1 and 10 ng/ml resulted in consistently enhanced levels of secreted and newly synthesised non-secreted proteins; supplementation with 100 and 1000 ng/ml resulted in lower or unchanged levels. These effects were most consistent 6 hr after a medium change. 2. Noradrenaline supplementation of the medium resulted in increased levels of secreted and non-secreted proteins at low concentrations (less than 2.4 ng/ml) at 6 hr after a medium change, but significantly decreased levels of both populations at high concentrations of noradrenaline (NA) (2.4 less than NA less than 1000 ng/ml), at 6-9 hr after a medium change and sustained to 24 hr, the most significant decrease being at 10 ng/ml. 3. All combinations of concentrations of A + NA resulted in non-dose-dependent decreased levels of both the secreted and non-secreted soluble protein fractions. The most significant decreases occurred at concentrations of (1 + 5) and (10 + 10) ng/ml adrenaline + noradrenaline. 4. Medium supplementation with adrenaline, noradrenaline or a combination of the two had no effect on the uptake of [3H]leucine by the cells. 5. The results are discussed in relation to receptor status on the hepatocyte membranes.
authors.affiliation: Department of General Physiology, School of Dentistry, University of the Witwatersrand, Johannesburg, South Africa.
authors.forename: M
authors.initials: M
authors.lastname: Silove
authors.forename: A L
authors.initials: AL
authors.lastname: Ritchie
authors.forename: J
authors.initials: J
authors.lastname: Hattingh
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004837:Epinephrine / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D011506:Proteins; D007930:Leucine; D009638:Norepinephrine; D004837:Epinephrine