A perspective on measurement of mutations in vivo.
The development of transgenic mouse lines with target genes that are suitable for tissue-specific mutagenesis studies is an important contribution to the field of environmental mutagenesis. These models can accommodate many of the questions relating to metabolism, distribution and relative potency of mutagens as well as providing a more comprehensive system for the identification of mutagens. However, the lesions that the field has learned about methods, development and validation from the use of in vitro systems must be applied to the development and validation of transgenic models.
Tennant
R W
RW
Experimental Carcinogenesis and Mutagenesis Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
D000818:Animals; D004252:DNA Mutational Analysis; D007763:Lac Operon; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics; D009152:Mutagenicity Tests / Q000379:methods*