title: Fine specificity of cytotoxic T lymphocytes primed in vivo either with virus or synthetic lipopeptide vaccine or primed in vitro with peptide.
abstract: Standard synthetic peptide preparations contain numerous peptidic byproducts in small amounts, which may be efficiently recognized by cytotoxic T lymphocytes (CTL). Recognition patterns of such peptide mixtures by CTL may serve as a kind of fingerprint for CTL fine specificity. Three types of H-2Db-restricted CTL were compared in this way. CTL primed in vivo either with A/PR/8/34 influenza virus or with a synthetic lipopeptide vaccine prepared from influenza nucleoprotein (NP) peptide 365-380 showed identical fine specificity. Both recognize virus-infected cells. In contrast, CTL primed in vitro with NP 365-380 had a different fine specificity and they did not recognize virus-infected cells. Most significantly, the two in vivo primed CTL types efficiently recognized the natural viral nonapeptide NP 366-374 presented by virus-infected H-2b cells, whereas the in vitro primed CTL failed to do so.
authors.affiliation: Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Schild
authors.forename: M
authors.initials: M
authors.lastname: Norda
authors.forename: K
authors.initials: K
authors.lastname: Deres
authors.forename: K
authors.initials: K
authors.lastname: Falk
authors.forename: O
authors.initials: O
authors.lastname: Rötzschke
authors.forename: K H
authors.initials: KH
authors.lastname: Wiesmüller
authors.forename: G
authors.initials: G
authors.lastname: Jung
authors.forename: H G
authors.initials: HG
authors.lastname: Rammensee
mesh_terms: D000818:Animals; D002460:Cell Line; D007252:Influenza Vaccines / Q000276:immunology*; D008074:Lipoproteins / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000276:immunology*; D009975:Orthomyxoviridae / Q000276:immunology*; D016601:RNA-Binding Proteins; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*; D014758:Viral Core Proteins / Q000276:immunology*
chemical_list: D007252:Influenza Vaccines; D008074:Lipoproteins; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D016601:RNA-Binding Proteins; D014614:Vaccines, Synthetic; D014758:Viral Core Proteins