[Electron microscopy of alpha-latrotoxin from the venom of the black widow spider Latrodectus mactans tredecimguttatus].
Two-dimensional crystals of alpha-latrotoxin from the venom of black widow spider (Latrodectus mactans tredecimguttatus) were studied by the negative staining electron microscopy. Two-dimensional crystals were obtained by adsorption of the protein solution with a high Mg2+ concentration on carbon-coated electron microscopy grids. The crystals were about 0.4 mkm in size, had the unit cell parameters: a = b = 15.55 nm, gamma = 90 degrees, p4 plane group symmetry. The contour map of a stain-excluding region of such crystals was calculated by the Fourier-filtering procedure at about 4 nm resolution. The calculation of molecular weight of the unit cell, with the symmetry p4 taken into account, showed that alpha-latrotoxin particles, revealed by negative staining, consisted of 4 or 8 protomers.
Lunev
A V
AV
Demin
V V
VV
Zaĭtsev
O I
OI
Spadir
S I
SI
Grishin
E V
EV
D003460:Crystallization; D008274:Magnesium / Q000737:chemistry; D008854:Microscopy, Electron; D013111:Spider Venoms / Q000737:chemistry*
D013111:Spider Venoms; C020763:alpha-latrotoxin; D008274:Magnesium
Elektronnaia mikroskopiia alpha-latrotokisina iz iada pauka karakurta Latrodectus mactans tredecimguttatus.