title: Urease (EC 3.5.1.5) inhibition in the sheep rumen and its effect on urea and nitrogen metabolism.
abstract: The urease (EC 3.5.1.5) inhibitor, phenylphosphoryldiamidate (PPDA), was given by continuous infusion into the rumen of two sheep nourished by intragastric infusion and into either the rumen or abomasum of four sheep given a pelleted diet containing 119 g crude protein (nitrogen x 6.25)/kg dry matter. PPDA was given at 1 g/d in infusion sheep and 1.5 g/d in the normally-fed sheep. Measurements of urea kinetics were made using single injections of [14C]urea. Urease inhibition was complete within 24 h of starting PPDA infusions to the rumen; in this time-period, urea concentration in rumen contents reached equilibrium with that in plasma and this situation persisted until infusions were terminated. Relative to the control periods, plasma urea and rumen ammonia concentrations were unchanged but urea irreversible loss rate decreased by 26% in infusion sheep and 33% in fed sheep when PPDA was given. Urinary urea excretion was not affected, hence urea degradation, measured by difference, decreased by 77 and 58% respectively in response to urease inhibition. Administration of PPDA to the abomasum resulted in a reduction in rumen urease activity to about 40% of control values but had no effect on urea metabolism. Differences between treatments in daily nitrogen retention were not significant, indicating that under the dietary conditions imposed in these experiments, even substantial changes in urea recycling had only minor effects on the overall N economy of the animal.
authors.affiliation: Rowett Research Institute, Bucksburn, Aberdeen.
authors.forename: F G
authors.initials: FG
authors.lastname: Whitelaw
authors.forename: J S
authors.initials: JS
authors.lastname: Milne
authors.forename: S A
authors.initials: SA
authors.lastname: Wright
mesh_terms: D000018:Abomasum / Q000187:drug effects; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D005260:Female; D008297:Male; D009584:Nitrogen / Q000378:metabolism*; D010755:Organophosphates / Q000008:administration & dosage*; D012417:Rumen / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D012756:Sheep / Q000378:metabolism*; D014508:Urea / Q000097:blood / Q000378:metabolism* / Q000493:pharmacokinetics; D014510:Urease / Q000037:antagonists & inhibitors*
chemical_list: D010755:Organophosphates; C032022:phosphoric phenyl ester diamide; D000641:Ammonia; D014508:Urea; D014510:Urease; D009584:Nitrogen