Idiometric assay, the third way: a noncompetitive immunoassay for small molecules.
A novel, noncompetitive immunoassay applicable to the measurement of small molecules including ovarian steroids is described. Using monoclonal antibodies with the ability to recognize both beta-typic and alpha-typic binding sites, a new simplified, sensitive, and specific immunoassay system has been developed. The initial work of this development is presented, along with preliminary results for a novel immunoassay for estradiol.
Barnard
G
G
Department of Chemistry, City University, London, United Kingdom.
Karsiliyan
H
H
Kohen
F
F
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D004958:Estradiol / Q000097:blood*; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D004958:Estradiol