title: [Rapid time-resolved fluoroimmunometric analysis of myoglobin].
abstract: High performance quantitative procedure for evaluation of myoglobin in blood serum is developed. The principle of two-site immunometry was used involving monoclonal antibodies against nonoverlapping epitopes of myoglobin molecule. The fluorescent compound containing europium served as a label of the antibody. Modification of the single-step assay enabled to increase the velocity of the assay to about 30 min and simplify it. The assay allowed estimation of myoglobin over a broad range of concentrations from 2 ng up to 1,000 ng/ml. The assay may be recommended for use in clinical practice for express diagnosis of myocardium infarction.
authors.forename: E A
authors.initials: EA
authors.lastname: Gracheva
authors.forename: M V
authors.initials: MV
authors.lastname: Nechaeva
authors.forename: V V
authors.initials: VV
authors.lastname: Roshke
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Rykova
authors.forename: G V
authors.initials: GV
authors.lastname: Shishkin
authors.forename: A V
authors.initials: AV
authors.lastname: Bredikhin
mesh_terms: D005455:Fluorescent Antibody Technique; D006801:Humans; D009203:Myocardial Infarction / Q000175:diagnosis; D009211:Myoglobin / Q000097:blood*
chemical_list: D009211:Myoglobin
vernacular_title: Bystryĭ vremiarazreshennyĭ fliuoroimmunometricheskiĭ analiz na mioglobin.