title: T-lymphocyte-receptor repertoire of infiltrating T lymphocytes into NOD mouse pancreas.
abstract: This study analyzed T-lymphocyte-receptor V beta genes of infiltrating lymphocytes into the pancreases of 12- to 16-wk-old NOD mice with severe insulitis and 5-wk-old mice with mild insulitis by the quantitative polymerase chain reaction method. The V beta transcripts on infiltrating T lymphocytes into pancreases with severe insulitis in older NOD mice were diverse. In contrast, the V beta 11 gene transcript was predominantly expressed on T lymphocytes in the pancreas in younger NOD mice with mild insulitis, suggesting the possible role of V beta 11+ T lymphocytes in triggering insulitis in this species.
authors.affiliation: Second Department of Internal Medicine, School of Medicine, Chiba University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Maeda
authors.forename: T
authors.initials: T
authors.lastname: Sumida
authors.forename: K
authors.initials: K
authors.lastname: Kurasawa
authors.forename: H
authors.initials: H
authors.lastname: Tomioka
authors.forename: I
authors.initials: I
authors.lastname: Itoh
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Koike
mesh_terms: D000818:Animals; D001483:Base Sequence; D015870:Gene Expression; D051379:Mice; D016688:Mice, Inbred NOD / Q000276:immunology*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010179:Pancreas / Q000276:immunology*; D010182:Pancreatic Diseases / Q000276:immunology; D016133:Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014158:Transcription, Genetic
chemical_list: D009838:Oligodeoxyribonucleotides; D011948:Receptors, Antigen, T-Cell