Release kinetics of ATP in cells exposed to nonionic detergents.
We have previously shown that the protein binding of intracellular ATP could be examined by monitoring the ATP release kinetics from Triton X-100 and Brij 58 nonionic detergent permeabilized cells. We have now analysed the protein binding of ATP in an isotonic medium using intact and partially ATP depleted Brij 58 treated human erythrocytes. The effects of Triton X-100 below the critical micelle concentration (CMC) was studied in normal and tumorous tissue culture cells and human red blood cells. Our results showed that the protein association of ATP was altered in the partially ATP depleted erythrocytes. Below the CMC value, but above a critical level Triton X-100 treatment was effective in mobilizing the intracellular ATP in both cell types. The ATP release curves were sigmoidal and an 'all or none' type of response was observed, especially in erythrocytes. The use of Triton X-100 (less than CMC) delays the detergent-induced cell decomposition time thus providing a new approach to investigating the physical state of intracellular ATP.
Köszegi
T
T
Department of Clinical Chemistry, Medical University of Pécs, Hungary.
D000255:Adenosine Triphosphate / Q000097:blood / Q000378:metabolism*; D002592:Cetomacrogol / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008823:Micelles; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D011485:Protein Binding
D003902:Detergents; D008823:Micelles; D011092:Polyethylene Glycols; D000255:Adenosine Triphosphate; D017830:Octoxynol; D002592:Cetomacrogol