title: A cDNA encoding RAP74, a general initiation factor for transcription by RNA polymerase II.
abstract: RAP30/74 (also known as TFIIF, beta gamma and FC is one of several general factors required for initiation by RNA polymerase II. The small RAP30 subunit of RAP30/74 binds directly to polymerase and appears structurally and functionally homologous to bacterial sigma factors in their RNA polymerase-binding region. RAP30/74 or recombinant RAP30 suppresses nonspecific binding of RNA polymerase II to DNA and is required for RNA polymerase II to assemble stably into a preinitiation complex containing promoter DNA and the general factors TFIID, TFIIA and TFIIB; both RAP30 and RAP74 are physical components of the preinitiation complex. A complementary DNA encoding human RAP30 has been isolated, and here we report the isolation of a cDNA encoding human RAP74. RAP30 and RAP74 produced in Escherichia coli can be used in place of natural human RAP30/74 to direct accurate transcription initiation by RNA polymerase II in vitro.
authors.affiliation: Department of Biochemistry, Michigan State University, E. Lansing 48824.
authors.forename: A
authors.initials: A
authors.lastname: Finkelstein
authors.forename: C F
authors.initials: CF
authors.lastname: Kostrub
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: D P
authors.initials: DP
authors.lastname: Chavez
authors.forename: B Q
authors.initials: BQ
authors.lastname: Wang
authors.forename: S M
authors.initials: SM
authors.lastname: Fang
authors.forename: J
authors.initials: J
authors.lastname: Greenblatt
authors.forename: Z F
authors.initials: ZF
authors.lastname: Burton
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002467:Cell Nucleus / Q000502:physiology; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D012319:RNA Polymerase II / Q000378:metabolism*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D019473:Transcription Factors, TFII; D014158:Transcription, Genetic
chemical_list: D004273:DNA, Neoplasm; D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D012319:RNA Polymerase II; C056604:transcription factor TFIIF