Autoantibodies against Forssman glycolipids in Graves' disease and Hashimoto's thyroiditis.
Sera from patients with Graves' disease and Hashimoto's thyroiditis have been shown to react with the Forssman glycolipid antigen (Gb5) using the techniques of high performance thin-layer chromatography (HPTLC) immunostaining and ELISA. Human monoclonal antibodies (MoAbs) have been prepared by fusion of human myeloma with peripheral lymphocytes from patients with Graves' disease. A MoAb, TRMo-4, reacted strongly and specifically with Gb5. These results suggest that anti-Forssman antibody may be involved in the pathogenesis of these autoimmune diseases. The detection of anti-Forssman glycolipid antibody may provide a useful means for clinical diagnosis and therapy.
Ariga
T
T
Department of Biochemistry and Molecular Biophysics, Medical College of Virginia, Richmond 23298-0614.
Yoshida
T
T
Mimori
T
T
Yu
R K
RK
D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000032:analysis*; D002518:Ceramides / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005577:Forssman Antigen / Q000276:immunology*; D005732:Gangliosides / Q000276:immunology; D005915:Globosides / Q000276:immunology*; D006111:Graves Disease / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D011989:Receptors, Thyrotropin; D011993:Recombinant Fusion Proteins; D013967:Thyroiditis, Autoimmune / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D002518:Ceramides; D005732:Gangliosides; D005915:Globosides; D007074:Immunoglobulin G; D011989:Receptors, Thyrotropin; D011993:Recombinant Fusion Proteins; C010163:Forssman glycolipid; D005577:Forssman Antigen