title: Differences in effects of oncogenes on resistance of gamma rays, ultraviolet light, and heat shock.
abstract: The effects of viral or activated cellular oncogenes on sensitivity to gamma rays, ultraviolet light, and heat shock were examined in SHOK (Syrian hamster Osaka-Kanazawa) cells and their transfectants. Resistance to gamma rays was conferred by the introduction of v-mos or c-cot genes, which coded serine/threonine kinase. Cells transfected with v-mos and c-cot genes increased their resistance to ultraviolet light and heat shock compared to their parent cells (SHOK cells). Of the activated ras genes, the N-ras gene developed a SHOK cell phenotype resistant to gamma rays and ultraviolet light. The Ha-ras gene produced SHOK cells resistant to ultraviolet light and heat shock, while introduction of the Ki-ras gene did not affect sensitivity. The v-erbB gene was found to be involved in the development of resistance to heat shock. Transfection with neo, c-myc, and v-fgr genes had little or no effect on cell survival. The karyotypes of SHOK cells and oncogene-containing cells were compared. No alterations were seen after the introduction of a foreign gene. Using cell cycle analysis, we found no apparent difference between SHOK cells and their transfectants. These results suggest that activation of serine/threonine kinase may be involved in common processes occurring after gamma-ray, ultraviolet-light, and heat-shock treatment, and that each oncogene may have a different effect on the development of a resistant phenotype.
authors.affiliation: Division of Radiation Biology, School of Medicine, Yokohama City University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: J
authors.initials: J
authors.lastname: Miyoshi
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D002588:Cesium Radioisotopes; D006224:Cricetinae; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006358:Hot Temperature / Q000009:adverse effects*; D008647:Mesocricetus; D009857:Oncogenes / Q000235:genetics*; D011836:Radiation Tolerance / Q000235:genetics*; D014466:Ultraviolet Rays
chemical_list: D002588:Cesium Radioisotopes