Translocation and induction of alpha B crystallin by heat shock in rat glioma (GA-1) cells.
Response to heat shock of alpha B crystallin expressed in rat astrocytoma GA-1 cells was analysed quantitatively using an immunoassay method. GA-1 cells contained a considerable amount of alpha B crystallin at growing phase. When the growing cells were heated at 45 degrees C, concentrations of alpha B crystallin in cell extracts were decreased to less than one-fifth of the original level within 15 min, with an increase in the insoluble fraction which was detected by immunoblotting. The low level of alpha B crystallin in the cytoplasm, that was observed for a few hours after heat shock, gradually recovered to the control level within several hours. At 10 h following heat shock (45 degrees C for 15 min), the concentration of alpha B crystallin in the soluble extract was about twice that of the control level, with little detectable amounts in the insoluble fraction. These results are additional evidence that suggest that alpha B crystallin is a small heat shock protein.
Inaguma
Y
Y
Department of Biochemistry, Aichi Prefectural Colony, Japan.
Shinohara
H
H
Goto
S
S
Kato
K
K
D000818:Animals; D002455:Cell Division; D003459:Crystallins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D005910:Glioma; D006358:Hot Temperature; D015151:Immunoblotting; D007700:Kinetics; D051381:Rats; D014407:Tumor Cells, Cultured
D003459:Crystallins