Common antigenic properties of a g-type (goose) and a c-type (duck) egg white lysozyme: antibody responses in rabbits and mice.
Embden goose (GEWL) and Barbary duck (DEWL) egg white lysozymes possess different amino acid sequences corresponding to the g-type and c-type, respectively. GEWL was shown to be a better immunogen than DEWL in both rabbits and mice. The antigenicity of the two lysozymes was tested using different techniques (i.e. indirect ELISA, inhibition tests and immunoabsorption experiments). Injection of either GEWL or DEWL into rabbits and mice induced both specific antibodies and cross-reacting antibodies. Moreover, anti-GEWL antibodies, in contrast to anti-DEWL antibodies, did not cross-react with hen egg white lysozyme (HEWL), a c-type lysozyme. While the structure of GEWL was not modified after binding to plastic, DEWL was denaturated, but it did keep some native epitopes. It was concluded that g-type and c-type lysozymes, which have different amino acid sequences, exhibit strong common antigenic properties.
Hemmen
F
F
Laboratoire d'immunologie, INRA Tours-Nouzilly, France.
Mahana
W
W
Jollès
P
P
Paraf
A
A
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D004372:Ducks; D004529:Egg White / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005777:Geese; D007106:Immune Sera / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D009113:Muramidase / Q000276:immunology*; D011817:Rabbits
D000941:Antigens; D007106:Immune Sera; D009113:Muramidase