title: Autoantibodies against Forssman glycolipids in Graves' disease and Hashimoto's thyroiditis.
abstract: Sera from patients with Graves' disease and Hashimoto's thyroiditis have been shown to react with the Forssman glycolipid antigen (Gb5) using the techniques of high performance thin-layer chromatography (HPTLC) immunostaining and ELISA. Human monoclonal antibodies (MoAbs) have been prepared by fusion of human myeloma with peripheral lymphocytes from patients with Graves' disease. A MoAb, TRMo-4, reacted strongly and specifically with Gb5. These results suggest that anti-Forssman antibody may be involved in the pathogenesis of these autoimmune diseases. The detection of anti-Forssman glycolipid antibody may provide a useful means for clinical diagnosis and therapy.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Medical College of Virginia, Richmond 23298-0614.
authors.forename: T
authors.initials: T
authors.lastname: Ariga
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Mimori
authors.forename: R K
authors.initials: RK
authors.lastname: Yu
mesh_terms: D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000032:analysis*; D002518:Ceramides / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005577:Forssman Antigen / Q000276:immunology*; D005732:Gangliosides / Q000276:immunology; D005915:Globosides / Q000276:immunology*; D006111:Graves Disease / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D011989:Receptors, Thyrotropin; D011993:Recombinant Fusion Proteins; D013967:Thyroiditis, Autoimmune / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D002518:Ceramides; D005732:Gangliosides; D005915:Globosides; D007074:Immunoglobulin G; D011989:Receptors, Thyrotropin; D011993:Recombinant Fusion Proteins; C010163:Forssman glycolipid; D005577:Forssman Antigen