The in vitro effects of lipid peroxidation on the content of individual forms of cytochrome P-450 in liver microsomes of guinea-pigs.
The effect of lipid peroxidation in vitro on the amounts of several forms of cytochrome P-450 in liver microsomes from guinea-pigs was investigated. Lipid peroxide formation in liver microsomes from ascorbic acid (VC)-deficient animals was much higher than that observed in control animals. The antibodies to rat P-450IA2 (P-448-H), P-450IIB1 (P-450b) and human P-450IIIA4 (P-450NF) recognized one or two forms of cytochrome P-450 in liver microsomes of guinea-pigs. Neither cytochrome P-450 cross-reactive with anti-P-450IIB1 antibodies nor cytochrome P-450 cross-reactive with antibodies to P-450IIIA4 was virtually affected by microsomal lipid peroxidation induced by NADPH in vitro. In contrast, the forms of cytochrome P-450 immunochemically related to P-450IA2 were decreased with the increased level of lipid peroxide formation. The form-specific degradation of cytochrome P-450 due to lipid peroxidation was in agreement with our previous observation that the amounts of cytochrome P-450 cross-reactive with antibodies to P-450IA2 but not with antibodies to P-450IIIA (P-450PB-1) were predominantly decreased in VC-deficient guinea-pigs compared to control animals in vitro.
Mori
T
T
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Kitada
M
M
Imaoka
S
S
Funae
Y
Y
Kamataki
T
T
D000818:Animals; D000918:Antibody Specificity; D001205:Ascorbic Acid / Q000378:metabolism / Q000494:pharmacology; D001206:Ascorbic Acid Deficiency / Q000201:enzymology; D015153:Blotting, Western; D003429:Cross Reactions; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D006168:Guinea Pigs; D006801:Humans; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats
D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System; D001205:Ascorbic Acid