title: Endothelin-1-induced vasoconstriction is not mediated by thromboxane release and action in the human fetal-placental circulation.
abstract: The vasoconstrictor peptide endothelin-1 (8 x 10(-10) to 1 x 10(-8) mol/L) significantly increased fetal-placental perfusion pressure in vitro in a cumulative manner from 30 +/- 2 to 123 +/- 25 mm Hg (mean +/- SEM, n = 5, p less than 0.0005, analysis of variance). Accompanying this vasoconstriction was a corresponding reduction in fetal-placental perfusate flow rate. Measurement of thromboxane B2 and 6-keto-prostaglandin F1 alpha in the fetal-placental perfusate revealed a significant reduction in their release (p less than 0.0096 and p less than 0.0004, analysis of variance, respectively) when corrected for flow rate. Neither the thromboxane synthesis inhibitor dazoxiben (10(-6) mol/L) nor the thromboxane receptor antagonist SQ29548 (10(-6) mol/L) was able to block the vasoconstrictor actions of endothelin-1. Therefore endothelin-1-induced vasoconstriction in the human fetal-placental circulation does not appear to be mediated by thromboxane release or action. The stimulus to eicosanoid release in the fetal-placental circulation may be hydrodynamic, i.e., flow or shear stress.
authors.affiliation: Department of Obstetrics and Gynecology, University of Cincinnati College of Medicine, OH 45267-0526.
authors.forename: L
authors.initials: L
authors.lastname: Myatt
authors.forename: G
authors.initials: G
authors.lastname: Langdon
authors.forename: A S
authors.initials: AS
authors.lastname: Brewer
authors.forename: D E
authors.initials: DE
authors.lastname: Brockman
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism* / Q000502:physiology; D016232:Endothelins / Q000502:physiology*; D006801:Humans; D012039:Regional Blood Flow; D013929:Thromboxane B2 / Q000378:metabolism* / Q000502:physiology; D014469:Umbilical Arteries / Q000502:physiology*; D014471:Umbilical Veins / Q000502:physiology*; D014661:Vasoconstriction
chemical_list: D016232:Endothelins; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha