title: Formation and resolution of recombination intermediates by E. coli RecA and RuvC proteins.
abstract: The recombination of DNA molecules has been reconstituted in vitro using two purified enzymes from Escherichia coli. RecA protein catalyses homologous pairing and strand exchange reactions to form intermediate DNA structures that are acted upon by RuvC. The newly identified RuvC protein resolves the intermediates by specific endonucleolytic cleavage to produce recombinant DNA molecules.
authors.affiliation: Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK.
authors.forename: H J
authors.initials: HJ
authors.lastname: Dunderdale
authors.forename: F E
authors.initials: FE
authors.lastname: Benson
authors.forename: C A
authors.initials: CA
authors.lastname: Parsons
authors.forename: G J
authors.initials: GJ
authors.lastname: Sharples
authors.forename: R G
authors.initials: RG
authors.lastname: Lloyd
authors.forename: S C
authors.initials: SC
authors.lastname: West
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004706:Endodeoxyribonucleases; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D046911:Macromolecular Substances; D008957:Models, Genetic; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D011938:Rec A Recombinases / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; C069806:ruvC protein, E coli; D011938:Rec A Recombinases; D004706:Endodeoxyribonucleases