title: Monoclonal antibodies against soman: characterization of soman stereoisomers.
abstract: Hybridomas were produced which expressed monoclonal anti-soman antibodies as determined by microtiter enzyme-linked-antibody immunoassay (EIA). Each of these antibodies was titrated using a competitive inhibition enzyme immunoassay (CIEIA) with a variety of test ligands. The ligands used included soman (a racemic mixture), sarin, tabun, and each of the four stereoisomers of soman (C+ P+, C+ P-, C-P+ and C-P-). In all cases the antibodies tested exhibited IC50 values of 10(-4)-5 x 10(-6) M for soman. When sarin or tabun was used as a ligand, the antibodies exhibited no cross reactivity. All of the antibodies cross reacted with the four soman stereoisomers. A second group of hybridomas were produced which expressed monoclonal antibodies against CsPs-soman. These antibodies were used to make preliminary absolute chiral assignments to the four soman stereoisomers.
authors.affiliation: United States Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD 21010-5425.
authors.forename: D E
authors.initials: DE
authors.lastname: Lenz
authors.forename: J J
authors.initials: JJ
authors.lastname: Yourick
authors.forename: J S
authors.initials: JS
authors.lastname: Dawson
authors.forename: J
authors.initials: J
authors.lastname: Scott
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D006241:Haptens / Q000737:chemistry; D051379:Mice; D012999:Soman / Q000737:chemistry / Q000276:immunology*; D013237:Stereoisomerism
chemical_list: D000911:Antibodies, Monoclonal; D006241:Haptens; D012999:Soman