Tissue specificity of S14 and fatty acid synthase in vitro transcription.
Hepatic nuclear extracts support in vitro transcription from S14 and fatty acid synthase (FAS) promoters. Renal nuclear extracts support in vitro transcription from S14 promoter constructs at rates comparable to hepatic nuclear extracts, but lack the apparent positive and negative control observed with liver. In contrast, renal nuclear extracts do not support in vitro transcription from the FAS promoter. NF-1 or a related protein in both hepatic and renal extracts contributes to in vitro transcriptional activity from the S14 promoter, but not the FAS promoter. These studies indicate that different mechanisms regulate tissue-specific expression of S14 and FAS.
MacDougald
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OA
Physiology Department, Michigan State University, East Lansing 48824.
Clarke
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SD
Jump
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DB
D000818:Animals; D002467:Cell Nucleus / Q000502:physiology*; D064429:Fatty Acid Synthases / Q000235:genetics*; D015723:Gene Library; D007668:Kidney / Q000502:physiology*; D007700:Kinetics; D008099:Liver / Q000502:physiology*; D009687:Nuclear Proteins; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D051381:Rats; D013154:Spleen / Q000502:physiology; D014157:Transcription Factors; D014158:Transcription, Genetic
D009687:Nuclear Proteins; D011506:Proteins; C051065:THRSP protein, human; C501478:Thrsp protein, rat; D014157:Transcription Factors; D064429:Fatty Acid Synthases