title: SrfI, a new type-II restriction endonuclease that recognizes the octanucleotide sequence, [sequence: see text].
abstract: A new restriction endonuclease, SrfI has been isolated from an unidentified species of Streptomyces. SrfI recognizes the 8-bp palindrome, 5'-GCCCGGGC and cleaves double-stranded DNA after the third C in the sequence, producing blunt ends. SrfI is a rare-cutting enzyme and should therefore be useful for megabase mapping.
authors.affiliation: Stratagene Cloning Systems, Inc., La Jolla, CA 92037.
authors.forename: T G
authors.initials: TG
authors.lastname: Simcox
authors.forename: S J
authors.initials: SJ
authors.lastname: Marsh
authors.forename: E A
authors.initials: EA
authors.lastname: Gross
authors.forename: W
authors.initials: W
authors.lastname: Lernhardt
authors.forename: S
authors.initials: S
authors.lastname: Davis
authors.forename: M E
authors.initials: ME
authors.lastname: Simcox
mesh_terms: D000256:Adenoviridae / Q000378:metabolism; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D009841:Oligonucleotides / Q000737:chemistry*; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D004279:DNA, Viral; D009841:Oligonucleotides; D015252:Deoxyribonucleases, Type II Site-Specific