The expression of proteins and activities of metabolic enzymes in transplanted brain tissue.
The middle three-fifths of the forebrains of 14-day-old embryos were obtained and transplanted into the cortical cavities of adult rats made 7 days prior to the transplantation. The expression of proteins, as revealed by 2-dimensional gel electrophoresis studies, and the activities of energy metabolizing enzymes in the mature allografts were compared with those in the 14-day-old embryonic forebrains and corresponding areas in the contralateral cerebral hemispheres of the hosts. They were shown to approach adult pattern and adult values after 10-12 weeks of growth. The biochemical findings were discussed and correlated with some of the anatomical observations.
Leong
S F
SF
Department of Physiology, National University of Singapore, Kent Ridge.
Leung
T K
TK
Leong
S K
SK
D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D016380:Brain Tissue Transplantation; D004734:Energy Metabolism; D016332:Fetal Tissue Transplantation; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D009419:Nerve Tissue Proteins