In vivo metabolism of fluorescent ceramide in central nervous system myelin of adult rats.
The metabolism of sphingolipids in the central nervous system (CNS) has been studied in adult rats by intraventricular administration of fluorescent ceramide (CER). Rats were sacrificed at various time points post inoculation and the fluorescence of CER, cerebrosides (CB), sulfatides (SULF) and sphingomyelin (SPM) was determined in the CNS myelin and in the pellet, containing mainly microsomes, obtained by Norton myelin preparation. The incorporation of fluorescence was more in the pellet than in the myelin at all times studied. Initially the fluorescence present in the pellet was prevalently due to untransformed CER but an increase of fluorescent products with time was observed. CB was the main product up to 2 h post inoculation, then it decreased with concomitant increase of fluorescent SULF. In the myelin we did not observe differences in incorporation and transformation of fluorescent CER with time: CB was the main fluorescent product at all times studied. At 0.5 h post inoculation the fluorescence, observed by fluorescence microscope, was located in the cell lining the ventricles while after 24 h it appeared also in the paraventricular areas.
Di Biase
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Department of Metabolism and Pathological Biochemistry, Istituto Superiore di Sanità, Viale Regina Elena, Rome, Italy.
Argiolas
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Confaloni
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Salvati
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D000818:Animals; D002518:Ceramides / Q000378:metabolism*; D002552:Cerebral Ventricles; D007263:Infusions, Parenteral; D007700:Kinetics; D050356:Lipid Metabolism; D008297:Male; D008856:Microscopy, Fluorescence; D009186:Myelin Sheath / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D002518:Ceramides