title: Phospholipid acylation by mouse sciatic nerve microsomes.
abstract: The partition of 0.3 nmol of [1-14C]oleoyl-CoA in the microsomes (10 micrograms proteins) from mouse sciatic nerves is unaffected by the presence of lysophospholipids and is about 45% of the total oleoyl-CoA (77% of the acylglycerophosphocholine partition in the membrane). The concentration of both oleoyl-CoA and acylglycerophosphocholine is over 1 mM in the membrane. There is a selective acyl transfer from acyl-CoA to lysolipid acceptors (oleoyl greater than myristoyl, palmitoyl, stearoyl much greater than eicosanoyl greater than docosanoyl, tetracosanoyl). The exogenous acyl acceptors are acylglycerophosphocholine and acylglycerophosphoinositol and to a lesser extent acylglycerophosphoethanolamine, but not acylglycerophosphoserine. A PC formation from acylGPC in the absence of exogenous acyl donors or from oleoyl-CoA in the absence of exogenous acyl acceptor was also observed.
authors.affiliation: Institut de Biochimie Cellulaire et Neurochimie du CNRS, Université de Bordeaux II, France.
authors.forename: C
authors.initials: C
authors.lastname: Cassagne
authors.forename: H
authors.initials: H
authors.lastname: Juguelin
authors.forename: F
authors.initials: F
authors.lastname: Boiron
mesh_terms: D000215:Acylation; D000818:Animals; D002384:Catalysis; D002462:Cell Membrane / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D008861:Microsomes / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D012584:Sciatic Nerve / Q000378:metabolism*
chemical_list: D010743:Phospholipids