title: Changes in binding activity of luteinizing hormone receptors by site directed mutagenesis of potential glycosylation sites.
abstract: Site directed mutagenesis of the rat ovarian luteinizing hormone (LH) receptor cDNA was performed at each of the six potential N-linked glycosylation sites to determine the effect of putative carbohydrate chains on the activity of the membrane receptor. The conversion of Asn173 to Gln resulted in the total loss of hormone binding to the surface of the transfected cell. Mutant receptors synthesized with substitutions at the remaining potential N-linked glycosylation positions of 77, 152, 269, 277 and 291 revealed no significant change in the hormone affinity. However Asn77Gln and Asn152Gln exhibited significant decreases (approximately 80%) in the number of high affinity hormone binding sites. The changes in hormone binding activity upon elimination of the potential glycosylation sites at 77, 152 and 173 indicate the presence of functional carbohydrate chains at these positions in the rat ovarian LH/hCG receptor.
authors.affiliation: Section on Molecular Endocrinology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: R
authors.initials: R
authors.lastname: Zhang
authors.forename: C H
authors.initials: CH
authors.lastname: Tsai-Morris
authors.forename: M
authors.initials: M
authors.lastname: Kitamura
authors.forename: E
authors.initials: E
authors.lastname: Buczko
authors.forename: M L
authors.initials: ML
authors.lastname: Dufau
mesh_terms: D000818:Animals; D001216:Asparagine; D001665:Binding Sites; D002236:Carbohydrate Conformation; D002460:Cell Line; D005260:Female; D005822:Genetic Vectors; D005973:Glutamine; D006031:Glycosylation; D006801:Humans; D016297:Mutagenesis, Site-Directed; D010053:Ovary / Q000737:chemistry; D011487:Protein Conformation; D051381:Rats; D011974:Receptors, LH / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D011974:Receptors, LH; D005973:Glutamine; D001216:Asparagine