The medium chains of the mammalian clathrin-associated proteins have a homolog in yeast.
We have cloned and sequenced mouse brain AP47, the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The predicted protein sequence of AP47 is closely related to rat and calf brain AP50, the corresponding medium chain of the plasma-membrane clathrin-associated protein complex AP-2. We have also identified in the yeast genome an open reading frame encoding a protein of previously unknown function. Referred to here as YAP54, its predicted protein sequence displays a striking homology to AP47. We therefore propose that Yap54 is the medium chain subunit of a putative AP-1 complex in yeast. From the analyses of the optimized sequence alignments of AP47, AP50 and Yap54p, we suggest a model for the domain organization of the medium chains.
Nakayama
Y
Y
Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, MA 02115.
Goebl
M
M
O'Brine Greco
B
B
Lemmon
S
S
Pingchang Chow
E
E
Kirchhausen
T
T
D033962:Adaptor Protein Complex 2; D033983:Adaptor Protein Complex mu Subunits; D033942:Adaptor Proteins, Vesicular Transport; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002417:Cattle; D002966:Clathrin / Q000378:metabolism*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005656:Fungal Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment
D033962:Adaptor Protein Complex 2; D033983:Adaptor Protein Complex mu Subunits; D033942:Adaptor Proteins, Vesicular Transport; D002966:Clathrin; D005656:Fungal Proteins; D010750:Phosphoproteins; C438885:adaptor protein complex 2, mu 1 subunit; C438886:adaptor protein complex 2, mu 2 subunit; D004247:DNA