The use of the polymerase chain reaction to identify porcine isolates of Trichinella.
A method was developed to identify domestic isolates of Trichinella using the polymerase chain reaction. Oligonucleotide primers, based on the repetitive DNA sequence (pPRA) from the P1 isolate of Trichinella, were used to amplify genomic DNA from 13 domestic isolates and tested against sylvatic isolates of Trichinella. Pattern differences were observed among domestic isolates, indicating divergence of this repetitive sequence. The primers were specific for domestic Trichinella as no amplification was detected for sylvatic isolates or Trichinella pseudospiralis. It was possible to identify an isolate from a single larva following digestion or in situ in muscle tissue.
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Department of Zoology, University of Manitoba, Winnipeg, Canada.
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deVos
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D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D007814:Larva / Q000145:classification / Q000302:isolation & purification; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000469:parasitology; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology*; D014234:Trichinella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014235:Trichinellosis / Q000469:parasitology / Q000662:veterinary*
D004247:DNA