[Immunoinhibitory determination of CK-MM subbands by monoclonal antibody].
The measurement of CK-MM isoform by monoclonal antibody and its clinical application were summarized. The tissue type of CK-MM3 isoform was completely inhibited, MM2 isoform was about 57% and the serum type of CK-MM1 isoform was not inhibited by use of monoclonal antibody. This method enabled selective immunoinhibitory measurement of tissue type of CK-MM isoform. The heat lability and inhibition by EDTA suggested that it is a metal-depending enzyme. The analysis of MM isoform offers a promising alternate, non-invasive method to detect and follow up AMI and successful reperfusion. The MM3/MM1 ratio has been found to peak at about 2 to 6 hours, making it a quicker responding parameter to AMI than MM3 alone and significantly more responsive than the total CK or CK-MB. An MM3/MM1 ratio greater than 1.0 appears to be a practical cutoff point for detection of the pathological release of MM3 from tissue. Reperfusion therapy should be instituted within about 2 to 4 hours of AMI onset.
Okabe
H
H
Department of Laboratory Medicine, Kumamoto University Medical School.
Uji
Y
Y
Sugiuchi
H
H
D000911:Antibodies, Monoclonal / Q000276:immunology; D004796:Clinical Enzyme Tests; D003402:Creatine Kinase / Q000097:blood* / Q000276:immunology; D006801:Humans; D007159:Immunologic Tests; D007527:Isoenzymes; D009203:Myocardial Infarction / Q000175:diagnosis*
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D003402:Creatine Kinase