title: The circular dichroism of tumor necrosis factor-alpha: measurement into the vacuum UV and analysis for secondary structure.
abstract: The circular dichroism (CD) spectrum of tumor necrosis factor-alpha has been measured into the vacuum UV to 168 nm. Analysis of the CD for secondary structure is in good agreement with X-ray diffraction results, but the analysis is somewhat unstable. Adding the CD of this protein together with its X-ray determined secondary structure to the basis set should improve subsequent analyses of CD spectra for other all-beta proteins.
authors.affiliation: Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331.
authors.forename: J H
authors.initials: JH
authors.lastname: Riazance-Lawrence
authors.forename: A
authors.initials: A
authors.lastname: Toumadje
authors.forename: W C
authors.initials: WC
authors.lastname: Johnson
mesh_terms: D002942:Circular Dichroism; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry*
chemical_list: D014409:Tumor Necrosis Factor-alpha