Interaction of ribosomal protein S1 and initiation factor IF3 with the 3' major domain and the decoding site of the 30S subunit of Escherichia coli.
We have studied the effect of the binding of ribosomal protein S1 and initiation factor IF3 on the accessibility of nucleotide residues 584-1506 in the small subunit of the Escherichia coli ribosome. Protein S1 strongly decreases RNase V1 attack at G1164, in hairpin 40 of the 3' major domain, and weakly decreases DMS attack at C1302, in the central loop of the 3' major domain, and at A1503, in the 3' minor domain. It also weakly increases the DMS reactivity of A1004, in the 3' major domain, and of A901, in the central domain. Factor IF3 strongly decreases RNase V1 attack (but not dimethyl sulfate attack) at A1408, in the decoding site, and weakly protects A1500, in the 3' minor domain and near the colicin E3 cleavage site. Neomycin does not interfere with this effect of IF3, but IF3 interferes with the protective effect of neomycin against dimethyl sulfate attack at A1408.
Laughrea
M
M
Lady Davis Institute for Medical Research, Sir Mortimer B. Davis--Jewish General Hospital, Montreal, Quebec, Canada.
Tam
J
J
D001483:Base Sequence; D004722:Endoribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry*; D008969:Molecular Sequence Data; D009355:Neomycin / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D010448:Peptide Initiation Factors / Q000378:metabolism* / Q000494:pharmacology; D039665:Prokaryotic Initiation Factor-3; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism* / Q000494:pharmacology; D012270:Ribosomes / Q000737:chemistry*; D013463:Sulfuric Acid Esters / Q000494:pharmacology
D010448:Peptide Initiation Factors; D039665:Prokaryotic Initiation Factor-3; D012336:RNA, Ribosomal, 16S; D012269:Ribosomal Proteins; D013463:Sulfuric Acid Esters; C015494:ribosomal protein S1; D004722:Endoribonucleases; C032825:ribonuclease V(1); D009355:Neomycin; C007482:dimethyl sulfate