title: Electrochromatographic solid-phase extraction for determination of cimetidine in serum by micellar electrokinetic capillary chromatography.
abstract: A highly effective electrochromatographic solid-phase extraction and preconcentration method is reported for the determination of cimetidine in serum in the concentration range 0.233-11.4 micrograms/ml. Preconcentrated samples were determined by micellar electrokinetic capillary chromatography while ranitidine was used as an internal standard. Sample preparation included retention of the analyte on a C18 solid-phase cartridge, followed by elution assisted by an applied voltage of 150 V. From 0.5-ml serum samples, 20-50-microliters aliquots were collected for electrophoretic analysis. Within the studied concentration range, the method was linear and provided adequate precision.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington 47405.
authors.forename: H
authors.initials: H
authors.lastname: Soini
authors.forename: T
authors.initials: T
authors.lastname: Tsuda
authors.forename: M V
authors.initials: MV
authors.lastname: Novotny
mesh_terms: D002845:Chromatography / Q000379:methods*; D002927:Cimetidine / Q000097:blood*; D006801:Humans; D011899:Ranitidine / Q000097:blood
chemical_list: D002927:Cimetidine; D011899:Ranitidine