Brain natriuretic peptides in human plasma, spinal cord and cerebrospinal fluid.
In cases of heart failure, the plasma level of immunoreactive human brain natriuretic peptide (IR-hBNP) measured by radioimmunoassay increased 6- to 46-fold over normal level (4.1 +/- 1.0 pg/ml), depending upon the severity. These levels did not significantly correlate to the levels of IR-human atrial natriuretic peptide in individuals. A 4-kDa IR-hBNP, corresponding to authentic hBNP (1-32), was predominant in normal plasma. In cases of cardiac disease, larger molecules which were assumed to be precursors of the 4-kDa form were accumulated in plasma. IR-hBNP was detected in spinal cords (7-24 pg/mg protein) and cerebrospinal fluid (8-19 pg/ml) of patients with noncardiac diseases. The major molecular form corresponded to the hBNP (1-32).
Togashi
K
K
Department of Molecular Biology, Kitasato University School of Medicine, Tokyo, Japan.
Kameya
T
T
Ando
K
K
Marumo
F
F
Kawakami
M
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002845:Chromatography / Q000379:methods; D005260:Female; D006333:Heart Failure / Q000097:blood / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009206:Myocardium / Q000378:metabolism; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011863:Radioimmunoassay; D013116:Spinal Cord / Q000737:chemistry* / Q000378:metabolism
D009419:Nerve Tissue Proteins; D020097:Natriuretic Peptide, Brain