title: L. monocytogenes-induced actin assembly requires the actA gene product, a surface protein.
abstract: The intracellular pathogenic bacterium L. monocytogenes can spread directly from cell to cell without leaving the cytoplasm. The mechanism of this movement, generated through bacterially induced actin polymerization, is not understood. By analyzing an avirulent Tn917-lac mutant defective for actin polymerization, we have identified a bacterial component involved in this process. The transposon had inserted in actA, the second gene of an operon. Gene disruption of downstream genes and transformation of the mutant strain with actA showed that the actA gene encodes a surface protein necessary for bacterially induced actin assembly. Our results indicate that it is a 610 amino acid protein with an apparent molecular weight of 90 kd.
authors.affiliation: Laboratoire de Génétique Moléculaire des Listeria, Institut Pasteur, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Kocks
authors.forename: E
authors.initials: E
authors.lastname: Gouin
authors.forename: M
authors.initials: M
authors.lastname: Tabouret
authors.forename: P
authors.initials: P
authors.lastname: Berche
authors.forename: H
authors.initials: H
authors.lastname: Ohayon
authors.forename: P
authors.initials: P
authors.lastname: Cossart
mesh_terms: D016475:3T3 Cells; D000199:Actins / Q000096:biosynthesis*; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D010450:Endopeptidases / Q000235:genetics; D005798:Genes, Bacterial; D008089:Listeria monocytogenes / Q000235:genetics* / Q000472:pathogenicity; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D010740:Phospholipases / Q000235:genetics; D014774:Virulence / Q000235:genetics
chemical_list: D000199:Actins; D001426:Bacterial Proteins; D008565:Membrane Proteins; D009841:Oligonucleotides; C072980:actA protein, Listeria monocytogenes; D010740:Phospholipases; D010450:Endopeptidases