Single-sweep polarographic investigation of concanavalin A and its interaction with polysaccharide.
A simple, rapid, and sensitive single-sweep polarographic method has been developed for investigation of concanavalin A (con A) and its interaction with selected polysaccharides. In a solution containing 0.001 M 2,2'-bipyridine, 0.015 M hexamethylenetetramine, and 0.1 M sodium chloride, con A exhibits a single-sweep polarographic wave, and the cathodic peak potential is -1.50 V (vs SCE). The peak current varies linearly with con A concentration over a range of 1.0 x 10(-8) to 1.2 x 10(-7) M by derivative single sweep polarography. A preliminary discussion on properties of the con A polarographic wave has been made. In addition, it has been demonstrated that single sweep polarography can be a useful method for studies on interactions of con A with its complementary polysaccharides in solution.
Liu
J
J
Department of Chemistry, Wuhan University, People's Republic of China.
Zeng
B Z
BZ
Lu
X D
XD
Zhou
X Y
XY
Zhao
Z F
ZF
D003208:Concanavalin A / Q000187:drug effects*; D011048:Polarography / Q000379:methods*; D011134:Polysaccharides / Q000494:pharmacology*
D011134:Polysaccharides; D003208:Concanavalin A