title: Rejuvenation of erythrocytes preserved with AS-1 and AS-3.
abstract: Rejuvenation of erythrocytes (biochemical modification of adenosine triphosphate and 2,3-diphosphoglycerate) preserved with currently available nutrient additive solutions has not been licensed or adequately studied. The ability of the commercially available solution PIPA to modify erythrocytes preserved with AS-1 or AS-3 for 35 and 42 days was studied. Use of PIPA produced greater-than-normal levels of adenosine triphosphate in the preserved erythrocytes. Restoration of 2,3-diphosphoglycerate was suboptimal at both periods. (PIPA treatment produces normal levels of 2,3-diphosphoglycerate in erythrocytes preserved for 35 days with citrate-phosphate-dextrose-adenine-1.) In erythrocytes preserved with AS-1 or AS-3 for 42 days, 2,3-diphosphoglycerate returned to normal levels after a second PIPA rejuvenation. This study demonstrates that biochemical modification is possible in erythrocytes preserved with AS-1 or AS-3 for 42 days.
authors.affiliation: Section of Transfusion Medicine, Mayo Clinic, Rochester, Minnesota 55905.
authors.forename: M E
authors.initials: ME
authors.lastname: Brecher
authors.forename: V W
authors.initials: VW
authors.lastname: Zylstra-Halling
authors.forename: A A
authors.initials: AA
authors.lastname: Pineda
mesh_terms: D019794:2,3-Diphosphoglycerate; D000225:Adenine / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000097:blood*; D001793:Blood Preservation; D002951:Citrates / Q000494:pharmacology; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007288:Inosine / Q000494:pharmacology; D008353:Mannitol / Q000494:pharmacology*; D010710:Phosphates / Q000494:pharmacology; D011773:Pyruvates / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology*; D013997:Time Factors
chemical_list: C037839:ADSOL; C058647:AS-3 preservative solution; D002951:Citrates; D004163:Diphosphoglyceric Acids; D010710:Phosphates; D011773:Pyruvates; D019794:2,3-Diphosphoglycerate; D008353:Mannitol; D012965:Sodium Chloride; D007288:Inosine; D000255:Adenosine Triphosphate; D005947:Glucose; D000225:Adenine