title: Immunochemical localization of extracellular materials in bone marrow of rats.
abstract: The distribution of type I collagen, fibronectin, laminin, and heparan sulfate was studied in marrow of rats by indirect immunofluorescence. Most of the type I collagen of marrow is associated with large blood vessels and connective tissue trabeculae, but type I collagen was also localized in a delicate meshwork throughout the marrow and in the basement membrane of the sinusoidal endothelium. Fibronectin is partially co-distributed with type I collagen, but is much more widely distributed. Sheets or septa of fibronectin-rich material divide the marrow into small compartments that contain and appear to separate clusters of developing blood cells. These septa may serve as a substrate for anchorage and migration of blood cells. Labeling of laminin was observed in the basement membranes of blood vessels, of fat cells, and of the sinusoidal wall, but only scattered labeling was seen in other extracellular materials. Heparan sulfate proteoglycan was poorly labeled in the extracellular matrix of marrow.
authors.affiliation: Department of Anatomical Sciences and Neurobiology, University of Louisville, Kentucky 40292.
authors.forename: R
authors.initials: R
authors.lastname: Hamilton
authors.forename: F R
authors.initials: FR
authors.lastname: Campbell
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000737:chemistry*; D003094:Collagen / Q000032:analysis; D004727:Endothelium / Q000737:chemistry; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D005353:Fibronectins / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006497:Heparan Sulfate / Q000032:analysis; D007106:Immune Sera; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D003094:Collagen; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D006497:Heparan Sulfate; D007106:Immune Sera; D007797:Laminin