[Obtaining human recombinant (serine-17) beta-interferon by the method of oligonucleotide-directed mutagenesis and its expression in Escherichia coli].
The gene of human mutant (serine-17) fibroblast interferon was isolated with the use of highly efficient oligonucleotide-directed mutagenesis. On the basis of the constructed expression plasmid pPR-IFN Ser17 a strain producing human mutant beta-interferon (VKPM V-4678) was developed. It was shown that the specific activity of the human mutant (serine-17) fibroblast interferon was 1 order of magnitude higher than that of the recombinant interferon which reaches the specific activity of natural fibroblast interferon.
Shekhter
I I
II
Beĭko
V P
VP
Bulenkov
M T
MT
Khodova
O M
OM
Kolevatykh
M A
MA
Izotova
L S
LS
Lebedeva
M I
MI
Rudenskiĭ
A Iu
AIu
Borukhov
S I
SI
Iurin
V I
VI
D001709:Biotechnology / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D000068556:Interferon beta-1a; D000068576:Interferon beta-1b; D016899:Interferon-beta / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D016297:Mutagenesis, Site-Directed / Q000235:genetics*
D000068576:Interferon beta-1b; D016899:Interferon-beta; D000068556:Interferon beta-1a
Poluchenie rekombinantnogo (serin-17) beta-interferona cheloveka metodom oligonukleotidnapravlennogo mutageneza i ego ékspresiia v Escherichia coli.