Structural studies on the [Bu(t)-Cys18](19-37)-fragment of human beta-calcitonin-gene-related peptide.
High-field n.m.r. studies were undertaken upon a peptide fragment of the C-terminal region of human beta-calcitonin-gene-related peptide (beta-hCGRP). Studies on the antigenic [Bu(t)-Cys18]beta-hCGRP-(19-37)-fragment revealed that several elements of secondary structure were present when the peptide was dissolved in [2H6]dimethyl sulphoxide. In particular an unspecified turn in the region of Ser19-Gly20 and a type I beta-turn in the region of Asn31-Val32-Gly33 were identified. Through-space connections between the terminal Phe37 amide group and the beta-protons of Thr50 suggest that the peptide may be folded into a loop-type conformation. These structural elements appear to overlap with the epitopes of a number of monoclonal antibodies and provide a molecular basis for understanding the role of the terminal Phe37 amide residue in the immune recognition of beta-hCGRP.
Sagoo
J K
JK
Department of Pharmaceutical Sciences, University of Nottingham, U.K.
Bose
C
C
Beeley
N R
NR
Tendler
S J
SJ
D000595:Amino Acid Sequence; D015740:Calcitonin Gene-Related Peptide / Q000031:analogs & derivatives* / Q000737:chemistry*; D006801:Humans; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry*; D011487:Protein Conformation
D046911:Macromolecular Substances; D010446:Peptide Fragments; C073807:calcitonin gene-related peptide (19-37), t-butyl-Cys(18)-; D015740:Calcitonin Gene-Related Peptide