Study of the solid phase extraction of pentoxifylline and its major metabolite as a basis of their rapid low concentration gas chromatographic determination in serum.
A gas-liquid chromatographic method for the determination of pentoxifylline and its secondary alcohol metabolite in serum has been developed. The method is based on the combination of solid phase extraction, capillary column separation and nitrogen-phosphorus detection of the analytes. Optimization of the solid phase extraction conditions permitted a low concentration determination, with limits of determination of 2 ng/mL and 10 ng/mL for pentoxifylline and its metabolite, respectively. The simplicity and rapidity of the extraction step was preserved.
Marko
V
V
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava.
Bauerová
K
K
D002849:Chromatography, Gas / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D010431:Pentoxifylline / Q000031:analogs & derivatives* / Q000097:blood*
C025189:lisofylline; D010431:Pentoxifylline