In vitro evaluation of a high-efficiency leukocyte adherence filter.
In view of the currently available data, prevention of alloimmunization requires filters with higher efficiency to achieve a reduction in the number of leukocytes below 10 million per transfusion. Two versions (Pall PL-100 and PL-50) of the new generation leukocyte-depletion filters were studied. Single donor (SDPC)- and pooled multiple donor (MDPC) platelets were run in parallel. At a flow rate of 10 ml/min, the PL-100 filter was shown to effectively reduce the number of residual leukocytes to far below the critical immunogenic threshold of 10 million in all SDPC units and in 77% of MDPC units. Apheresis platelets appear not only to be better depleted than pooled multiple donor platelets, but also to have a better post-filtration platelet recovery (96% versus 84%). The efficiency of the smaller version of the filter (Pall-50) was higher than that of the Pall-100 filter for both single and pooled multiple donor platelet concentrates (PC). Leukocytes were absent in more than 92% of units in both types of concentrates. The maximal number of detected leukocytes was 2.2 million in a pool of 6 units. The outcome of filtration of 5-day-old pooled platelets was less favorable than filtration of 1- or 2-day-old pooled platelets, indicating that filtration soon after preparation is preferred to filtration after storage. Post-filtration platelet integrity, activation state, function, and morphology were all well preserved in both single and multiple PCs.
Elias
M K
MK
Red Cross Blood Bank Groningen-Drenthe, Groningen, The Netherlands.
Smit
J W
JW
Weggemans
M
M
Rijskamp
L
L
Carper
H
H
McShine
R L
RL
Brons
R
R
Pietens
J
J
Halie
M R
MR
Smit Sibinga
C T
CT
D001781:Blood Component Removal / Q000295:instrumentation* / Q000592:standards; D002448:Cell Adhesion; D005069:Evaluation Studies as Topic; D005374:Filtration / Q000295:instrumentation*; D005434:Flow Cytometry; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000502:physiology*