Isolation and identification of methylarginines from bovine brain.
Methylarginines in free form were identified in bovine brain. Three compounds were isolated from the basic aliphatic amino acid fraction of bovine brain with several ion-exchange chromatographies. They showed the same Rf values in paper and thin-layer chromatographies as those of authentic NG-monomethylarginine, NG,NG-dimethylarginine, and NG,N'G-dimethylarginine. The migration distance of the isolated compounds in high-voltage paper electrophoresis and the retention times in ion-exchange HPLC were also identical to those of the above authentic methylarginines. We concluded that these three compounds are the methyl derivatives of arginine described above. The amount of these three compounds isolated from 1,090 g of bovine brain was 0.3 mumol of NG-monomethylarginine, 0.1 mumol of NG,NG-dimethylarginine, and 0.5 mumol of NG,N'G-dimethylarginine. The occurrence of these free methylarginines may have an important role in regulating the signal transduction through the nitric oxide system.
Kotani
K
K
Department of Neuropsychiatry, Ehime University School of Medicine, Japan.
Ueno
S
S
Sano
A
A
Kakimoto
Y
Y
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004590:Electrophoresis, Paper; D019323:omega-N-Methylarginine
D019323:omega-N-Methylarginine; C018524:N,N-dimethylarginine; D001120:Arginine