Melatonin modifies calmodulin cell levels in MDCK and N1E-115 cell lines and inhibits phosphodiesterase activity in vitro.
The interaction between melatonin and calmodulin was explored. Calmodulin cell levels in MDCK and N1E-115 cells cultured with 10(-9) M melatonin were increased after 3 days but decreased after 6 days. Melatonin inhibited calmodulin-dependent phosphodiesterase and when either melatonin or [3H]melatonin was preincubated with calmodulin and separated by electrophoresis, comigration of calmodulin with the radioactivity as well as modification of the Ca2+ calmodulin shift were observed. The results point out that one of the mechanisms of action of melatonin is a calmodulin-melatonin interaction.
Benítez-King
G
G
Departamento de Neurofarmacología, Instituto Mexicano de Psiquiatría, México.
Huerto-Delgadillo
L
L
Antón-Tay
F
F
D000818:Animals; D002147:Calmodulin / Q000096:biosynthesis / Q000378:metabolism*; D002460:Cell Line; D004285:Dogs; D004586:Electrophoresis; D008550:Melatonin / Q000494:pharmacology*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*
D002147:Calmodulin; D010726:Phosphodiesterase Inhibitors; D008550:Melatonin