Production of an antiserum using a fusion protein produced by a cDNA for rat choline acetyltransferase.
A fusion protein produced by a cDNA for rat choline acetyltransferase (ChAT) inserted into a translation vector was used for immunization of rabbits. An antiserum was obtained that could recognize a single protein band in immunoblot analysis of a partially purified enzyme preparation of the rat brain. The antiserum revealed ChAT immunoreactivity in the motoneurons and terminal-like structures in the neuropil of the ventral horn in cryostat sections of the cervical spinal cord of the rat. This antiserum may be of particular use to study the development of the cholinergic neuron.
Ichikawa
T
T
Department of Anatomy and Embryology, Tokyo Metropolitan Institute for Neurosciences, Japan.
Ohsako
S
S
Deguchi
T
T
D000818:Animals; D002795:Choline O-Acetyltransferase / Q000096:biosynthesis* / Q000235:genetics; D004247:DNA / Q000378:metabolism*; D015245:Deoxyribonuclease BamHI / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D007150:Immunohistochemistry; D008297:Male; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology*; D013116:Spinal Cord / Q000276:immunology / Q000378:metabolism; D013564:Sympathetic Nervous System / Q000276:immunology / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D004247:DNA; D002795:Choline O-Acetyltransferase; D015245:Deoxyribonuclease BamHI