Heat inactivation of DNA ligase prior to electroporation increases transformation efficiency.
Ymer
S
S
Medical Molecular Biology Group, John Curtin School of Medical Research, Australian National University, Canberra City, ACT.
D011088:DNA Ligases / Q000037:antagonists & inhibitors*; D004270:DNA, Circular / Q000235:genetics; D004278:DNA, Superhelical / Q000235:genetics; D004558:Electric Stimulation; D005822:Genetic Vectors / Q000235:genetics; D006358:Hot Temperature; D010957:Plasmids / Q000235:genetics; D014169:Transformation, Bacterial / Q000235:genetics*
D004270:DNA, Circular; D004278:DNA, Superhelical; D011088:DNA Ligases