Column liquid chromatographic determination of carbadox and olaquindox in feeds.
A column liquid chromatographic method for simultaneous determination of carbadox and olaquindox in swine feeds is described. The drugs were extracted from feeds with carbon tetrachloride-dimethylformamide (80:20) at 60 degrees C for 30 min. The extract was mixed with water (25:45). After centrifugation the aqueous layer was chromatographed on a reversed-phase column using gradient elution and ultraviolet detection at wavelengths of 305 and 262 nm. Recoveries from samples fortified at levels of 20-50 ppm were 92 +/- 9% for carbadox and 93 +/- 6% for olaquindox (means +/- standard deviations, n = 71).
dos Ramos
F J
FJ
Laboratório de Bromatologia e Nutrição, Faculdade de Farmácia da Universidade de Coimbra, Portugal.
da Silveira
I N
IN
de Graaf
G
G
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002218:Carbadox / Q000032:analysis*; D002251:Carbon Tetrachloride; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004126:Dimethylformamide; D011810:Quinoxalines / Q000032:analysis*; D013552:Swine
D011810:Quinoxalines; D004126:Dimethylformamide; D002251:Carbon Tetrachloride; C013168:olaquindox; D002218:Carbadox