title: 2-Deoxy-2-fluoro-D-galactose protein N-glycosylation.
abstract: 2-Deoxy-2-fluoro-D-galactose (dGalF), added to the medium of primary cultured rat hepatocytes, inhibited N-glycosylation of membrane (gp 120) and secretory glycoproteins (alpha 1-macroglobulin) in a concentration-dependent manner. Complete inhibition of N-glycosylation was achieved at concentrations of 1 mM and above. At identical concentrations, 2-deoxy-2-fluoro-D-glucose (dGlcF) caused only incomplete inhibition of N-glycosylation. dGalF reduced incorporation of D-[2,6-3H]mannose into lipid-linked oligosaccharides indicating interference with their assembly in the dolichol cycle.
authors.affiliation: Institut für Molekularbiologie und Biochemie Freien Universität Berlin, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Loch
authors.forename: C C
authors.initials: CC
authors.lastname: Geilen
authors.forename: I
authors.initials: I
authors.lastname: Spörndle
authors.forename: F
authors.initials: F
authors.lastname: Oberdorfer
authors.forename: D
authors.initials: D
authors.lastname: Keppler
authors.forename: R
authors.initials: R
authors.lastname: Tauber
authors.forename: W
authors.initials: W
authors.lastname: Reutter
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003847:Deoxyglucose / Q000031:analogs & derivatives / Q000494:pharmacology; D004288:Dolichol Phosphates / Q000378:metabolism; D019788:Fluorodeoxyglucose F18; D005643:Fucose / Q000031:analogs & derivatives* / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism; D051381:Rats; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D004288:Dolichol Phosphates; D006023:Glycoproteins; D008565:Membrane Proteins; D009844:Oligosaccharides; D019788:Fluorodeoxyglucose F18; D014415:Tunicamycin; D005643:Fucose; D003847:Deoxyglucose; C049289:2-deoxy-2-fluorogalactose; D008358:Mannose