[Comparison of lipid metabolism in rat liver cells and nuclei during cycloheximide-induced superinduction of nuclear oncogenes].
Using a model of cycloheximide (CHI)-induced expression of nuclear oncogens, a comparative study of metabolism of the major lipid classes in rat liver nuclei and cells was carried out. A short-term activation of sphingomyelinase which preceded on a time scale the maximal accumulation of c-fos and c-myc transcripts was observed both in the cells and in the nuclei. In contrast with the whole cell, the level of phospholipase C activity in the nuclei did not change under conditions of oncogene activation. It was found that the maximal expression of nuclear oncogens coincided in time with cyclic changes in the content of practically all phospholipids and neutral lipids with simultaneous activation of their synthesis both in the cells and in the nuclei. However, in the nuclei the sphingomyelin metabolism activation was predominant. It is concluded that in the nucleus sphingomyelin and its metabolites may influence oncogene expression via nuclear protein kinase C.
Filippova
G N
GN
Borovkova
O V
OV
Alesenko
A V
AV
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D002518:Ceramides / Q000096:biosynthesis / Q000378:metabolism; D003513:Cycloheximide / Q000633:toxicity*; D004789:Enzyme Activation; D015870:Gene Expression; D016259:Genes, myc; D006868:Hydrolysis; D050356:Lipid Metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D010743:Phospholipids / Q000096:biosynthesis / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D011519:Proto-Oncogenes; D051381:Rats; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism
D002518:Ceramides; D010743:Phospholipids; D016760:Proto-Oncogene Proteins c-fos; D003513:Cycloheximide; D010738:Type C Phospholipases; D013108:Sphingomyelin Phosphodiesterase
Sravnenie metabolizma lipidov v iadrakh i kletkakh pecheni krys v usloviiakh TsGI-indutsirovannoĭ superékspressii iadernykh onkogenov.