[Design of a species-specific DNA probe based on the pFra plasmid of the plague pathogen].
The recombinant plasmid pBS1 carrying a 2 kb SalGI fragment of Yersinia pestis pFra plasmid was constructed by insertion of the fragment into a vector plasmid pBR327. SalGI-BspRI 400 bp subfragment was recloned into a pBR322 vector plasmid. Open reading frame was found in the fragment by DNA sequencing technique. The subfragment designated F1-probe permits one to identify specifically the Yersinia pestis strains harbouring pFra plasmid, thus, differing them from closely related Yersiniea and other representatives of Enterobacteriaceae family.
Shishkina
O G
OG
Popov
Iu A
IuA
Kirillina
O A
OA
Mashko
S V
SV
Lebedeva
M I
MI
D000595:Amino Acid Sequence; D001483:Base Sequence; D015342:DNA Probes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D015183:Restriction Mapping; D013045:Species Specificity; D015010:Yersinia pestis / Q000235:genetics*
D015342:DNA Probes
Konstruirovanie vidospetsificheskogo DNK-zonda na osnove plazmidy pFra chumnogo mikroba.