NGF increases cortical acetylcholine release in rats with lesions of the nucleus basalis.
Rats received a unilateral lesion of the nucleus basalis by infusion of ibotenic acid. Two weeks after the lesion, osmotic minipumps were implanted, that infused 1 microgram human recombinant nerve growth factor (NGF) or cytochrome-C per day into the lateral ventricle. After four weeks of treatment, release of acetylcholine was measured in the frontal neocortex by means of in-vivo microdialysis. Release was decreased by 75% on the lesioned side; perfusion with 100 mM KCl increased release on the intact side by 130% and on the lesioned side by 80%. Treatment with NGF increased release on the lesioned side twofold, but had no effect on release on the intact side.
Dekker
A J
AJ
Department of Neurosciences, University of California San Diego, La Jolla 92161.
Langdon
D J
DJ
Gage
F H
FH
Thal
L J
LJ
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000502:physiology*; D002794:Choline / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D003956:Dialysis / Q000379:methods; D007839:Functional Laterality; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology; D008297:Male; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010296:Parietal Lobe / Q000187:drug effects / Q000502:physiology; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D011994:Recombinant Proteins / Q000494:pharmacology; D013377:Substantia Innominata / Q000502:physiology*
D009414:Nerve Growth Factors; D011994:Recombinant Proteins; D011189:Potassium Chloride; D002795:Choline O-Acetyltransferase; D002794:Choline; D000109:Acetylcholine