Expression of the nuclear protein mitotin in differentiating in vitro HL 60 cells.
Mitotin is a 125 kDa/pI 6.5 nuclear protein specific for proliferating cells and markedly increased prior to and during mitosis. This study presents evidence for the expression of this protein during dimethylsulfoxide (DMSO) induced differentiation of human promyelocytic leukemia HL 60 cells. The expression had been followed at two levels: as antigen, using a specific antimitotin monoclonal antibody and as mRNA, using a specific cDNA probe. The results from the immunofluorescent study show a gradual disappearance of mitotin in differentiating HL 60 cells starting from the fourth day after DMSO induction. On the other hand, the changes in the expression of mitotin mRNA were much more dramatic. This mRNA is expressed at a high level during the first three days of differentiation but shows a striking decrease after the fourth day. This correlates with the rapid changes in the number of blast cells in the differentiating HL 60 cell population. Therefore, the expression of mitotin mRNA can serve as a marker for the changes accompanying the termination of cell proliferation in differentiating cells.
Philipova
R N
RN
Institute of Cell Biology and Morphology, Sofia, Bulgaria.
Vassilev
A P
AP
Kaneva
R P
RP
Andreeva
P
P
Todorov
I T
IT
Hadjiolov
A A
AA
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000378:metabolism; D015152:Blotting, Northern; D002454:Cell Differentiation; D002455:Cell Division; D015342:DNA Probes; D004121:Dimethyl Sulfoxide; D005455:Fluorescent Antibody Technique; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000302:isolation & purification*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015342:DNA Probes; D009687:Nuclear Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D004121:Dimethyl Sulfoxide