title: [Biochemical, antigenic and protective properties of the outer membrane of tularemia pathogens].
abstract: The outer membranes of Francisella tularensis were studied. The membranes were identified morphologically, immunologically and biochemically. They contained 12-20% of protein, 15-30% of carbohydrates, up to 40% of lipids. The main integral proteins of the outer membranes were the 47, 43, 17 and 12 kD proteins. The main protein 63 kD was not integral. The lipopolysaccharides isolated from the outer membranes and acetone-dried cells did not possess the protective properties in experimental tularemia. The preparations of outer membranes possessed the protective properties for mice infected with the virulent strain 503. Chitosan amplified the protective properties of outer membranes.
authors.forename: V S
authors.initials: VS
authors.lastname: Khlebnikov
authors.forename: I R
authors.initials: IR
authors.lastname: Golovlev
authors.forename: D P
authors.initials: DP
authors.lastname: Kulevatskiĭ
authors.forename: S F
authors.initials: SF
authors.lastname: Averin
authors.forename: S Iu
authors.initials: SIu
authors.lastname: Pshirkov
authors.forename: N V
authors.initials: NV
authors.lastname: Tokhtamysheva
authors.forename: V E
authors.initials: VE
authors.lastname: Zhemchugov
authors.forename: L A
authors.initials: LA
authors.lastname: Safonova
authors.forename: V N
authors.initials: VN
authors.lastname: Gerasimov
authors.forename: A M
authors.initials: AM
authors.lastname: Chugunov
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines; D015153:Blotting, Western; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D002686:Chitin / Q000031:analogs & derivatives* / Q000378:metabolism; D048271:Chitosan; D004591:Electrophoresis, Polyacrylamide Gel; D005604:Francisella tularensis / Q000276:immunology / Q000378:metabolism*; D007106:Immune Sera; D007114:Immunization; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing; D008070:Lipopolysaccharides / Q000276:immunology / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D007106:Immune Sera; D008070:Lipopolysaccharides; D002686:Chitin; D048271:Chitosan
vernacular_title: Izuchenie biokhimicheskikh, antigennykh i protektivnykh svoĭstv vneshneĭ membrany vozbuditelia tuliaremii.