title: Gelatin particle indirect agglutination test for mass examination for strongyloidiasis.
abstract: An indirect agglutination test using recently developed gelatin particles was assessed to determine its applicability as a screening test for mass examination for strongyloidiasis. 1199 individuals in Sashiki Town, Okinawa Island, were screened by the test and 34.7% were determined to be antibody positive. Follow-up examination of the persons whose sera showed positive antibody responses demonstrated the presence of faecal larvae in 41.7%. The calculated infection rate (14.5%) was similar (14.1%) to that indicated by another survey using the micro-enzyme-linked immunosorbent assay (micro-ELISA), conducted simultaneously among the inhabitants. The indirect agglutination test was simple to perform in a short time and without specialized equipment. Additionally, the gelatin particles have many advantages as an antigen carrier, e.g. in handling, reading of the resulting pattern, and stable, long-term preservation. The test was considered to be more convenient than the micro-ELISA for mass screening for strongyloidiasis.
authors.affiliation: Department of Parasitology, School of Medicine, University of the Ryukyus, Okinawa, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Toma
authors.forename: S
authors.initials: S
authors.lastname: Kiyuna
authors.forename: Y
authors.initials: Y
authors.lastname: Shiroma
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000372:Agglutination Tests / Q000379:methods; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005780:Gelatin; D006801:Humans; D007564:Japan; D008297:Male; D008403:Mass Screening / Q000379:methods*; D008875:Middle Aged; D013321:Strongyloides / Q000276:immunology; D013322:Strongyloidiasis / Q000175:diagnosis / Q000453:epidemiology*
chemical_list: D000909:Antibodies, Helminth; D005780:Gelatin