title: Maximum oxygen uptake and arterial blood oxygenation during hypoxic exercise in rats.
abstract: The objectives of these experiments were 1) to describe the effect of maximum treadmill exercise on gas exchange, arterial blood gases, and arterial blood oxygenation in rats acclimated for 3 wk to simulated altitude (SA, barometric pressure 370-380 Torr) and 2) to determine the contribution of acid-base changes to the changes in arterial blood oxygenation of hypoxic exercise. Maximum O2 uptake (VO2max) was measured in four groups of rats: 1) normoxic controls run in normoxia (Nx), 2) normoxic controls run in acute hypoxia [AHx inspiratory PO2 (PIO2) approximately 70 Torr], 3) SA rats run in hypoxia (3WHx, PIO2 approximately 70 Torr), and 4) SA rats run in normoxia (ANx). VO2max (ml STPD.min-1.kg-1) was 70.8 +/- 0.9 in Nx, 46.4 +/- 1.9 in AHx, 52.6 +/- 1.1 in 3WHx, and 70.0 +/- 2.4 in ANx. Exercise resulted in acidosis, hypocapnia, and elevated blood lactate in all groups. Although blood lactate increased less in 3WHx and ANx, pH was the same or lower than in Nx and AHx, reflecting the low buffer capacity of SA. In AHx and 3WHx, arterial PO2 increased with exercise; however, O2 saturation of hemoglobin in arterial blood (SaO2) decreased. In vitro measurements of the Bohr shift suggest that SaO2 decreased as a result of a decrease in hemoglobin O2 affinity. The data indicate that several features of hypoxic exercise in this model are similar to those seen in humans, with the exception of the mechanism of decrease in SaO2, which, in humans, appears to be due to incomplete alveolar-capillary equilibration.
authors.affiliation: Department of Physiology, University of Kansas Medical Center, Kansas City 66103.
authors.forename: N C
authors.initials: NC
authors.lastname: Gonzalez
authors.forename: A
authors.initials: A
authors.lastname: Sokari
authors.forename: R L
authors.initials: RL
authors.lastname: Clancy
mesh_terms: D000064:Acclimatization / Q000502:physiology; D000136:Acid-Base Equilibrium / Q000502:physiology; D000531:Altitude; D000818:Animals; D001784:Blood Gas Analysis; D002245:Carbon Dioxide / Q000097:blood; D000860:Hypoxia / Q000097:blood*; D007773:Lactates / Q000097:blood; D008297:Male; D010100:Oxygen / Q000097:blood*; D010101:Oxygen Consumption / Q000502:physiology*; D010108:Oxyhemoglobins / Q000378:metabolism; D005082:Physical Exertion / Q000502:physiology*; D011659:Pulmonary Gas Exchange; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007773:Lactates; D010108:Oxyhemoglobins; D002245:Carbon Dioxide; D010100:Oxygen