Selection, hitchhiking and disequilibrium analysis at three linked loci with application to HLA data.
The HLA system has been extensively studied from an evolutionary perspective. Although it is clear that selection has acted on the genes in the HLA complex, the nature of this selection has yet to be fully clarified. A study of constrained disequilibrium values is presented that is applicable to HLA and other less polymorphic systems with three or more linked loci, with the purpose of identifying selection events. The method uses the fact that three locus systems impose additional constraints on the range of possible disequilibrium values for any pair of loci. We have thus examined the behavior of the normalized pairwise disequilibrium measures using two locus (D'), and also three locus (D"), constraints on pairwise disequilibria in a three locus system when one of the three loci is under positive selection. The difference between these measures, delta = magnitude of D' - magnitude of D", has a distribution for the two unselected loci differing from that for the selected locus with either of the unselected loci (the hallmark is a high positive value of delta for the two unselected loci). An examination of genetic drift indicates that positive delta values are unlikely to be found in human populations in the absence of selection when recombination is greater than about 0.1%. This measure can thus provide insight into which allele of several linked loci might have been subject to selection. Application of this method to HLA haplotypes from a large French population study (Provinces Francaise) identifies selected alleles on particular haplotypes. Application of a complementary method, disequilibrium pattern analysis also confirms the action of selection on these haplotypes.
Robinson
W P
WP
Department of Integrative Biology, University of California, Berkeley 94720.
Cambon-Thomsen
A
A
Borot
N
N
Klitz
W
W
Thomson
G
G
D000483:Alleles; D005787:Gene Frequency; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D008040:Genetic Linkage; D005828:Genetics, Population; D006680:HLA Antigens / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008285:Major Histocompatibility Complex; D008962:Models, Theoretical; D011110:Polymorphism, Genetic; D012641:Selection, Genetic
D006680:HLA Antigens