Mechanism of liver injury following ischemia.
To clarify whether ischemic liver injury is due to ischemia itself or reperfusion, histopathological and functional changes in the liver were examined before and after liver ischemia in rats with porto-systemic collateral channels. Effects of oxygen-derived free radical scavengers or an inhibitor of platelet aggregation on development of ischemic liver injury were also examined. Liver ischemia was produced by ligation of the portal vein and hepatic artery at liver hilum for 1 hr. The primary lesion of ischemic liver injury was cloudy swelling of liver cells in the periportal and midzonal regions; it developed during ischemia. The cloudy swelling of liver cells induced uneven distribution of sinusoidal blood flow after reperfusion, and consequently individual liver cell necrosis and focal hepatocellular necrosis in the midzonal regions developed later. Elevation of cytoplasmic enzyme activities in the serum after reperfusion was due to leakage across the damaged plasma membrane of liver cells. The treatment with superoxide dismutase, catalase, or heparin had not altered the liver injury that was attributed to ischemia, biochemically and histologically. These results suggest that ischemic liver injury is due to liver cell damage developed during ischemia, and that the ischemic liver injury is not alleviated or prevented by superoxide dismutase, catalase, or heparin.
Shibayama
Y
Y
Department of Pathology, Osaka Medical College, Japan.
Asaka
S
S
Nishijima
A
A
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D002374:Catalase / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D007511:Ischemia / Q000473:pathology*; D008099:Liver / Q000098:blood supply* / Q000187:drug effects / Q000473:pathology; D008111:Liver Function Tests; D008297:Male; D009336:Necrosis; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000473:pathology* / Q000517:prevention & control; D013482:Superoxide Dismutase / Q000494:pharmacology
D006493:Heparin; D002374:Catalase; D013482:Superoxide Dismutase; D000410:Alanine Transaminase