[Immunological study of lysates from the cell wall of group A Streptococcus].
The chemical composition and presence of immunogenic components in the lysates of the cell walls of group A Streptococcus, type M29, were studied. The lysates were prepared with the use of muramidase. Fc-Receptors were detected in the lysates. Within the first 30 minutes of cell wall lysis by muramidase, 4 times higher amounts of the protein reacting with fibrinogen excreted than in the subsequent 4 hours. The lysates contained immunogenic proteins. Fraction III isolated by chromatography of the 30-minute lysate on DEAE-trisacryl formed a single precipitation band with lysate antiserum. The lysate Fraction IV forming three precipitation bands contained a protein not specific of the type. The protein was identical to the protein antigen from Triton X-100 extracts of group A Streptococcus, types M1, M12 and M29. The group-specific polysaccharide was detected in the lysate Fraction I and Fraction II of the 4-hour lysate.
Shmakova
Z F
ZF
Shikhman
A R
AR
Briko
N I
NI
Kuznetsov
V D
VD
D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D002850:Chromatography, Gel / Q000379:methods; D003470:Culture Media; D006868:Hydrolysis / Q000187:drug effects; D005779:Immunodiffusion / Q000379:methods; D007122:Immunoelectrophoresis / Q000379:methods; D066298:In Vitro Techniques; D009113:Muramidase / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D013297:Streptococcus pyogenes / Q000378:metabolism*
D001426:Bacterial Proteins; D003470:Culture Media; D011135:Polysaccharides, Bacterial; D009113:Muramidase
Immunologicheskoe izuchenie lizatov kletochnoĭ stenki streptokokka gruppy A.