Killing of Burkitt-lymphoma-derived Daudi cells by ultraviolet-inactivated vaccinia virus.
Interaction of active and UV-inactivated vaccinia virus at high multiplicity caused cytological changes and inhibition in cellular protein and DNA synthesis, thus arresting the multiplication of Burkitt-lymphoma-derived Daudi cells and eventually killing the cells. Adsorption to the cells but the lack of penetration was evident by immunofluorescence, electron microscopy and [3H]thymidine-labeled virus incorporation. Viral DNA synthesis or virus replication was not demonstrated. Thus, it appears that the massive adsorption of viral particles, active or UV-inactivated, or possibly a "toxic" component that resides in the virion, damages the plasma membrane and may be responsible for killing the cells by a mechanism of lysis from without.
Grunwald-Beard
L
L
Department of Virology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Gamliel
H
H
Parag
G
G
Vedantham
S
S
Zakay-Rones
Z
Z
D000818:Animals; D002051:Burkitt Lymphoma / Q000235:genetics / Q000628:therapy*; D002460:Cell Line; D002463:Cell Membrane Permeability; D002470:Cell Survival; D002522:Chlorocebus aethiops; D003602:Cytotoxicity, Immunologic; D004247:DNA / Q000096:biosynthesis; D004307:Dose-Response Relationship, Radiation; D005455:Fluorescent Antibody Technique; D006801:Humans; D007167:Immunotherapy; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D014176:Protein Biosynthesis; D014466:Ultraviolet Rays; D015164:Vaccines, Inactivated / Q000494:pharmacology*; D014616:Vaccinia virus / Q000472:pathogenicity / Q000528:radiation effects
D015164:Vaccines, Inactivated; D004247:DNA