Assignment of the human heterogeneous nuclear ribonucleoprotein A1 gene (HNRPA1) to chromosome 12q13.1 by cDNA competitive in situ hybridization.
Heterogeneous nuclear ribonucleoprotein (HNRP) core protein A1 is a major component of mammalian HNRP particles. The human HNRP A1 protein was shown to be encoded by a 4.6-kb gene, split into 10 exons, belonging to a multigene family of about 30 A1-specific sequences per haploid genome, many of which correspond to pseudogenes of the processed type. Here we report the mapping of the human HNRPA1 gene to band 12q13.1. Localization was performed by nonisotopic in situ hybridization using a phage genomic clone that contains the active HNRPA1 gene as well as 13.5-kb flanking sequences. To suppress hybridization to pseudogene sequences, unlabeled HNRPA1 cDNA was added in excess over the probe to the hybridization mixture.
Saccone
S
S
Dipartimento di Genetica e Microbiologia A. Buzzati-Traverso, University of Pavia, Italy.
Biamonti
G
G
Maugeri
S
S
Bassi
M T
MT
Bunone
G
G
Riva
S
S
Della Valle
G
G
D001667:Binding, Competitive; D002478:Cells, Cultured; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D012332:RNA, Heterogeneous Nuclear / Q000235:genetics*; D012261:Ribonucleoproteins / Q000235:genetics*
D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D012332:RNA, Heterogeneous Nuclear; D012261:Ribonucleoproteins; C000619656:hnRNPA1 protein, human