title: DNA binding activity of recombinant SRY from normal males and XY females.
abstract: The protein encoded by the human testis determining gene, SRY, contains a high mobility group (HMG) box related to that present in the T cell-specific, DNA-binding protein TCF-1. Recombinant SRY protein was able to bind to the same core sequence AACAAAG recognized by TCF-1 in a sequence dependent manner. In five XY females point mutations were found in the region encoding the HMG box. In four cases DNA binding activity of mutant SRY protein was negligible; in the fifth case DNA binding was reduced. These results imply that the DNA binding activity of SRY is required for sex determination.
authors.affiliation: Human Molecular Genetics Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
authors.forename: V R
authors.initials: VR
authors.lastname: Harley
authors.forename: D I
authors.initials: DI
authors.lastname: Jackson
authors.forename: P J
authors.initials: PJ
authors.lastname: Hextall
authors.forename: J R
authors.initials: JR
authors.lastname: Hawkins
authors.forename: G D
authors.initials: GD
authors.lastname: Berkovitz
authors.forename: S
authors.initials: S
authors.lastname: Sockanathan
authors.forename: R
authors.initials: R
authors.lastname: Lovell-Badge
authors.forename: P N
authors.initials: PN
authors.lastname: Goodfellow
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins; C497745:SRY protein, human; D051876:Sex-Determining Region Y Protein; C497746:Sry protein, mouse; D014157:Transcription Factors