title: Calcium antagonists prevent monocyte and endothelial cell-induced modification of low density lipoproteins.
abstract: Low density lipoprotein (LDL) incubated in the presence of the calcium antagonists verapamil, nifedipine and flunarizine were more resistant than control LDL to human monocyte- or endothelial cell-induced modification, as assessed by electrophoretic mobility in agarose gel, thiobarbituric acid reactive substance content, and degradation by J774 macrophages. The efficiency of the drugs was: flunarizine greater than nifedipine greater than verapamil. Moreover, a 24 h preculture with calcium antagonists significantly impaired the ability of cells to modify LDL in the absence of the drugs. All the studied drugs also inhibited copper-induced autooxidation of LDL. None of the studied calcium antagonists, at concentrations up to 10(-4) M, significantly reacted with free radicals as assessed by the 1,1-diphenyl-2-picrylhydrazyl test. It is suggested that such a protective effect of calcium antagonists against LDL peroxidation could play a role in the previously reported antiatherogenic effect of these drugs.
authors.affiliation: Institut de Recherches Servier (IDRS), Suresnes, France.
authors.forename: C
authors.initials: C
authors.lastname: Breugnot
authors.forename: C
authors.initials: C
authors.lastname: Mazière
authors.forename: M
authors.initials: M
authors.lastname: Auclair
authors.forename: L
authors.initials: L
authors.lastname: Mora
authors.forename: M F
authors.initials: MF
authors.lastname: Ronveaux
authors.forename: S
authors.initials: S
authors.lastname: Salmon
authors.forename: R
authors.initials: R
authors.lastname: Santus
authors.forename: P
authors.initials: P
authors.lastname: Morlière
authors.forename: A
authors.initials: A
authors.lastname: Lenaers
authors.forename: J C
authors.initials: JC
authors.lastname: Mazière
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002478:Cells, Cultured; D003864:Depression, Chemical; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016166:Free Radical Scavengers; D006801:Humans; D007231:Infant, Newborn; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000378:metabolism*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000473:pathology; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D002121:Calcium Channel Blockers; D016166:Free Radical Scavengers; D008077:Lipoproteins, LDL; D014810:Vitamin E; D002118:Calcium