In situ hybridization analysis of ovarian prostaglandin endoperoxide synthase mRNA throughout the periovulatory period of the ewe.
Cellular alterations in level of expression of mRNA encoding for prostaglandin endoperoxide synthase were quantified within ovarian tissues of sheep obtained before, during and after induction of the preovulatory surge of LH and ovulation with LHRH. This was accomplished by isotopic in situ hybridization using a selective cRNA probe to ovine prostaglandin endoperoxide synthase mRNA. A significant elevation in mRNA was detected within the theca interna of the preovulatory follicle at 8, 16 and 24 hr following administration of LHRH. Very close to the time of ovulation (ie., at 24 hr post-LHRH) a marked rise in mRNA was observed in association with epithelial cells covering the apical surface of the follicle. Ovarian cyclooxygenase metabolites of arachidonic acid produced during the ovulatory process in the ewe originate within the thecal layer and germinal epithelium of the follicle destined to ovulate.
Murdoch
W J
WJ
Department of Animal Science, University of Wyoming, Laramie 82071.
Slaughter
R G
RG
Ji
T H
TH
D000818:Animals; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D006080:Ovarian Follicle / Q000502:physiology*; D010060:Ovulation / Q000502:physiology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics*; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis*; D012756:Sheep / Q000235:genetics / Q000502:physiology*
D015347:RNA Probes; D012333:RNA, Messenger; D007987:Gonadotropin-Releasing Hormone; D011451:Prostaglandin-Endoperoxide Synthases