title: [Determination of alcohol dehydrogenase genotype: no correlation between isoenzyme pattern and liver cirrhosis].
abstract: Mounting evidence from various fields of research links the oxidation product of alcohol, acetaldehyde, with the development of alcohol abuse-related pathology. One factor governing the production of acetaldehyde is the genetically determined pattern of class I alcohol dehydrogenase isoenzymes, consisting of "fast" beta 2 and gamma 1 and "slow" beta 1 and gamma 2 subunits. Alcoholics carrying the beta 2 and gamma 1 genes might, therefore, be more susceptible to alcohol-related liver disease. To verify this hypothesis we developed a method based on the polymerase chain reaction and restriction enzyme digestion in order to genotype individuals with respect to their alcohol dehydrogenase isoenzyme pattern. In a total of 100 caucasian individuals the following genotypes were determined: beta 1 beta 1, 92; beta 1 beta 2, 8; beta 2 beta 2 0; gamma 1 gamma 1, 38; gamma 1 gamma 2, 51; gamma 2 gamma 2, 11. No statistically significant differences in the distribution of isoenzymes were detectable between alcoholics with liver disease, patients with non-alcohol abuse-related liver disease, patients with rheumatoid arthritis and healthy controls.
authors.affiliation: Chemisches Zentrallabor Universitätskliniken, Inselspital, Bern.
authors.forename: B
authors.initials: B
authors.lastname: Wermuth
authors.forename: E
authors.initials: E
authors.lastname: Ernst
authors.forename: J P
authors.initials: JP
authors.lastname: von Wartburg
authors.forename: R
authors.initials: R
authors.lastname: Speck
authors.forename: F
authors.initials: F
authors.lastname: Schwarzenbach
authors.forename: B
authors.initials: B
authors.lastname: Lauterburg
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000595:Amino Acid Sequence; D001172:Arthritis, Rheumatoid / Q000201:enzymology; D005838:Genotype; D006521:Hepatitis, Chronic / Q000201:enzymology; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008108:Liver Diseases, Alcoholic / Q000201:enzymology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D007527:Isoenzymes; D000426:Alcohol Dehydrogenase
vernacular_title: Bestimmung des Alkoholdehydrogenase-Genotyps: keine Korrelation zwischen Isoenzymmuster und Leberzirrhose.