Walking, cloning, and mapping with yeast artificial chromosomes: a contig encompassing D21S13 and D21S16.
Chromosome 21 has often been used as a model system for the development of genome mapping and cloning strategies in humans. In this report methods for systematic chromosome walking, cloning, and mapping are exemplified in the construction of a 1.5-Mb yeast artificial chromosome (YAC) contig encompassing and extending 400 kb beyond each of the genetic loci D21S13 and D21S16. Isolation of insert-terminal sequences from YACs in this contig provides a set of closely spaced physical markers. These have been used to generate a long-range genomic restriction map.
Butler
R
R
Biotechnology Department, ICI Pharmaceuticals, Macclesfield, Cheshire, United Kingdom.
Ogilvie
D J
DJ
Elvin
P
P
Riley
J H
JH
Finniear
R S
RS
Slynn
G
G
Morten
J E
JE
Markham
A F
AF
Anand
R
R
D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D016386:Chromosome Walking; D015825:Chromosomes, Fungal; D002891:Chromosomes, Human, Pair 21; D003001:Cloning, Molecular; D004247:DNA; D015342:DNA Probes; D015226:Dinucleoside Phosphates / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D015723:Gene Library; D005819:Genetic Markers / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D004247:DNA; D015342:DNA Probes; D015226:Dinucleoside Phosphates; D005819:Genetic Markers; C015772:CpG dinucleotide