title: A melanoma octamer binding protein is responsive to differentiating agents.
abstract: Analysis of human melanocytes and melanoma cell lines for proteins interacting with the octamer control sequence (ATGCAAAT) has revealed two distinct melanoma octamer binding proteins, Oct-M1 and Oct-M2. The latter was restricted to cell lines derived from tumor metastases. The level of Oct-M1 activity in a pigmented melanoma line was enhanced in comparison to the general octamer binding protein Oct-1 when cells were cultured in the presence of the depigmenting agent dithiothreitol and conversely was reduced by the differentiating and pigment inducing agents butyric acid and dimethyl sulfoxide.
authors.affiliation: Centre for Molecular Biology and Biotechnology, University of Queensland, Australia.
authors.forename: R A
authors.initials: RA
authors.lastname: Sturm
authors.forename: F
authors.initials: F
authors.lastname: Bisshop
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: P G
authors.initials: PG
authors.lastname: Parsons
mesh_terms: D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002454:Cell Differentiation / Q000187:drug effects; D002461:Cell Line, Transformed; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D051863:Host Cell Factor C1; D006801:Humans; D008545:Melanoma / Q000378:metabolism*; D050811:Octamer Transcription Factor-1; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D002087:Butyrates; D004268:DNA-Binding Proteins; C497692:HCFC1 protein, human; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; C494165:POU2F1 protein, human; D014157:Transcription Factors; D020148:Butyric Acid; D004229:Dithiothreitol; D004121:Dimethyl Sulfoxide