Enzymatic peptide synthesis in organic solvent mediated by gels of copolymerized acrylic derivatives of alpha-chymotrypsin and polyoxyethylene.
Copolymers of acrylated derivatives of alpha-chymotrypsin and polyethylene glycol (PEG) have been prepared and used as biocatalysts for the synthesis of model peptides in organic solvent containing a low quantity of water. Other peptide couplings have been tried to point out the chemico- and stereoselectivity and examples of segment couplings are given.
Fulcrand
V
V
Laboratory for the Synthesis and Physiochemical Studies of Amino Acids and Peptides, URA 468, University of Montpellier II, France.
Jacquier
R
R
Lazaro
R
R
Viallefont
P
P
D000179:Acrylates / Q000737:chemistry; D000215:Acylation; D002384:Catalysis; D002918:Chymotrypsin / Q000737:chemistry*; D010452:Peptide Biosynthesis; D010455:Peptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013237:Stereoisomerism
D000179:Acrylates; D010455:Peptides; D011108:Polymers; D011092:Polyethylene Glycols; C026200:acryloyl chloride; D002918:Chymotrypsin