Folylpolyglutamate synthetase from beef liver: purification and some properties.
The polypeptide chain of folylpolyglutamate synthetase from beef liver has been isolated and partially characterized. This polypeptide has an apparent molecular weight of 73,000. Its amino-terminal residue is blocked. Amino acid analysis agrees with the hydrophobic properties and the pI (6.0) of this cytosolic enzyme. Polyclonal antibodies to the denatured enzyme have been prepared.
Pristupa
Z B
ZB
Department of Biochemistry, University of Toronto, Ont., Canada.
McAuley
C M
CM
Scrimgeour
K G
KG
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology*; D010453:Peptide Synthases / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*
D000596:Amino Acids; D010453:Peptide Synthases; C023103:folylpolyglutamate synthetase