title: Stress-induced gastric lesions and the synthesis of prostaglandins and leukotrienes.
abstract: Prostaglandin E2 renders the mucosa of the GI tract more resistant to acute injury by aspirin, acid, bile salts, and stress, whereas leukotrienes promote inflammation and retard healing of ulcers. The relationship of stress ulcer formation to changes in the activities of prostaglandin synthetase and lipoxygenase was evaluated in this study. After a 24 hr fast, 10 rats were stressed by the cold-restraint method for 4 hr and 10 rats were not stressed. Rats were terminated, stomachs were excised, the number of lesions were counted, and the nonulcerated mucosa was assayed for prostaglandin E2 and leukotriene synthesis. The mucosa was minced, washed in buffered saline, and then incubated for 10 min in a 1.3 mM sodium arachidonate solution containing a nonionic detergent (poloxamer 188), NaCl, KCl, KPO4, glutathione, hemin, MgSO4 and Hepes at pH 8.0. An ANOVA was used to compare the groups. Following stress the number of gastric lesions increased from 0.7 +/- 0.6 to 13.5 +/- 2.6, while leukotriene synthesis increased from 173 +/- 20 to 2170 +/- 187 pg/mg/min. A shift in synthesis from prostaglandins to leukotrienes in the mucosa appears to be detrimental to cytoprotection.
authors.affiliation: Department of Surgery, Long Island Jewish Medical Center, Albert Einstein College of Medicine, New Hyde Park, New York 11042.
authors.forename: T A
authors.initials: TA
authors.lastname: Stein
authors.forename: L M
authors.initials: LM
authors.lastname: Keegan
authors.forename: L J
authors.initials: LJ
authors.lastname: Auguste
authors.forename: B
authors.initials: B
authors.lastname: Bailey
authors.forename: L
authors.initials: L
authors.lastname: Wise
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology; D005753:Gastric Mucosa / Q000378:metabolism*; D015289:Leukotrienes / Q000096:biosynthesis*; D011453:Prostaglandins / Q000096:biosynthesis*; D051381:Rats; D013276:Stomach Ulcer / Q000209:etiology / Q000378:metabolism*; D013312:Stress, Physiological / Q000150:complications* / Q000378:metabolism
chemical_list: D015289:Leukotrienes; D011453:Prostaglandins; D002118:Calcium