Maintained cellular function of adrenal medullary cells in parkinsonian dysautonomia.
Adrenal gland involvement in Parkinson's disease was reported by different authors. Further studies became relevant after adrenal was proposed as dopaminergic donor for neurotransplantation. Chromaffin cells were grown in culture and the effects of nerve growth factor (NGF) tested: no differences were observed between parkinsonian and control cells. The expression of the beta-NGF mRNA in the parkinsonian adrenal was analyzed: a specific cDNA was synthesized and a 168 bp portion amplified using PCR. The products were identified and the identity of the fragment was confirmed by sequencing. Quantitative PCR demonstrated a beta-NGF mRNA concentration exceeding 5 fg/micrograms of total adrenal RNA. These findings demonstrate the retained functional capacity of the parkinsonian adrenal to respond to NGF and express the beta-NGF mRNA.
Falini
A
A
Institute of Neurology, University of Milan, Medical School, Italy.
Silani
V
V
Pizzuti
A
A
Rugarli
E I
EI
Mariani
D
D
Bassani
R
R
Vegeto
A
A
Baralle
F E
FE
Scarlato
G
G
D000313:Adrenal Medulla / Q000502:physiology*; D001342:Autonomic Nervous System Diseases / Q000503:physiopathology*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D015342:DNA Probes; D006801:Humans; D009414:Nerve Growth Factors / Q000502:physiology; D010300:Parkinson Disease / Q000503:physiopathology*; D016133:Polymerase Chain Reaction
D015342:DNA Probes; D009414:Nerve Growth Factors