title: Decrease of proliferation rate and induction of differentiation by a MYCN antisense DNA oligomer in a human neuroblastoma cell line.
abstract: The effects of an antisense oligodeoxynucleotide to codons 2-7 of the oncogene MYCN on the human neuroblastoma cell line LAN-5 were studied. Treated cells showed a decreased MYCN protein expression and synthesis by immunoperoxidase staining and immunoprecipitation. At the same time, the replication rate was inhibited, and the phenotype was modified toward a more differentiated type. Our data suggest the involvement of oncogene MYCN in both proliferative and differentiative processes.
authors.affiliation: Division of Physics and Biochemical Sciences, Ente per le nuove technologie per l'energia e l'ambiente (ENEA), Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Negroni
authors.forename: S
authors.initials: S
authors.lastname: Scarpa
authors.forename: A
authors.initials: A
authors.lastname: Romeo
authors.forename: S
authors.initials: S
authors.lastname: Ferrari
authors.forename: A
authors.initials: A
authors.lastname: Modesti
authors.forename: G
authors.initials: G
authors.lastname: Raschellà
mesh_terms: D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002455:Cell Division / Q000187:drug effects* / Q000235:genetics; D002461:Cell Line, Transformed; D004261:DNA Replication / Q000187:drug effects; D016373:DNA, Antisense / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D016259:Genes, myc / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D016900:Neurofilament Proteins / Q000032:analysis; D014746:Vimentin / Q000032:analysis
chemical_list: D016373:DNA, Antisense; D016900:Neurofilament Proteins; D014746:Vimentin