title: The in situ expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNA at the maternal-fetal interface.
abstract: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is produced by cells in the placenta, is known to be a growth factor for trophoblast cells in vitro and when injected into pregnant mice at risk for mid-gestation fetal resorption, dramatically lowers the fetal death rate while stimulating placental and fetal growth. We describe here the localization of GM-CSF mRNA expression in murine placenta by in situ hybridization. It is found in small round cells (lymphoid-like) and endothelial cells in the maternal decidua. In addition, GM-CSF transcripts are located in cells of the spongiotrophoblast zone (trophoblast-like cells), but not in the labyrinthine zone. These results indicate that GM-CSF may be influencing the growth and function of the fetal placenta in a paracrine-autocrine manner. These results support earlier observations that link GM-CSF production during pregnancy to decidual T-lymphocytes and further suggest a placental source within the invasive trophoblast.
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kanzaki
authors.forename: M
authors.initials: M
authors.lastname: Crainie
authors.forename: H
authors.initials: H
authors.lastname: Lin
authors.forename: J
authors.initials: J
authors.lastname: Yui
authors.forename: L J
authors.initials: LJ
authors.lastname: Guilbert
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: T G
authors.initials: TG
authors.lastname: Wegmann
mesh_terms: D000818:Animals; D003656:Decidua / Q000378:metabolism; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D009693:Nucleic Acid Hybridization; D010920:Placenta / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D014327:Trophoblasts / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D016178:Granulocyte-Macrophage Colony-Stimulating Factor