Crystals of isoenzyme 3-3 of rat liver glutathione S-transferase with and without inhibitor.
The isoenzyme 3-3 of rat liver glutathione S-transferase (GST 3-3) isolated from a baculovirus expression system has been crystallized with and without inhibitor. The crystals grown in the absence of an inhibitor belong to space group P2(1) with cell dimensions a = 119.7, b = 96.2, c = 136.7 A and beta = 103.3 degrees, and diffract to 3 A resolution. The crystals grown in the presence of an inhibitor belong to space group C2 with cell dimensions a = 88.3, b = 69.7, c = 81.4 A and beta = 105.3 degrees, and diffract to at least 2.5 A resolution. The inhibitor used is either methylmercury chloride or ethylmercury chloride; both are weak inhibitors.
Fu
J H
JH
Department of Crystallography, University of Pittsburgh, PA 15260.
Rose
J
J
Chung
Y J
YJ
Tam
M F
MF
Wang
B C
BC
D000818:Animals; D003461:Crystallography; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000737:chemistry*; D007527:Isoenzymes / Q000737:chemistry*; D008099:Liver / Q000201:enzymology*; D015394:Molecular Structure; D051381:Rats; D014961:X-Ray Diffraction
D007527:Isoenzymes; D005982:Glutathione Transferase