Simultaneous HPLC analysis of the hypnotic benzodiazepines nitrazepam, estazolam, flunitrazepam, and triazolam in plasma.
A new analytical methodology was developed for simultaneous high-performance liquid chromatographic quantitation of nitrazepam, estazolam, flunitrazepam, and triazolam in plasma. After addition of the internal standard nor-prazepam, biological samples and calibration standards were extracted at basic pH into diethyl ether-methylene chloride (2:1, v/v). The reconstituted extract was separated on a reversed-phase Nova Pak C18 column with acidic buffer (6mM)-acetonitrile-methanol (64:23:13, v/v) as mobile phase. The detection was performed at 242 nm. Within-run and day-to-day precision values were about 2-8%. The method is thus sensitive and reproducible for use in clinical and experimental toxicokinetic studies.
Boukhabza
A
A
Laboratoire de Toxicologie Fondamentale, Clinique et du Médicament, Faculté de Pharmacie, Strasbourg, France.
Lugnier
A A
AA
Kintz
P
P
Mangin
P
P
D002851:Chromatography, High Pressure Liquid; D004949:Estazolam / Q000097:blood*; D005445:Flunitrazepam / Q000097:blood*; D006801:Humans; D009567:Nitrazepam / Q000097:blood*; D014229:Triazolam / Q000097:blood*
D014229:Triazolam; D004949:Estazolam; D005445:Flunitrazepam; D009567:Nitrazepam