Catecholamine and neuropeptide Y levels in tissues from young Dahl rats following 5 days low- or high-salt diet.
The mesenteric vasculature of Dahl salt-sensitive (DS) rats on high-salt diet is supersensitive to nerve stimulation and to norepinephrine. The current experiments were undertaken to examine whether the enhanced sensitivity to nerve stimulation is due solely to the postsynaptic supersensitivity to norepinephrine, to increased sympathetic innervation, to altered transmitter release or to the presence of another transmitter acting as a potentiator. Catecholamine content and neuropeptide Y (NPY) presence were determined in tissues from young (approximately 5 weeks old) male Dahl rats exposed to 5 days of high (7%) or low (0.45%) salt diet. Catecholamine content from mesenteric artery, renal artery, caudal artery, right atrium, aorta, vas deferens and adrenal gland was quantified by high-pressure liquid chromatography with an electrochemical detector. A strain difference, independent of diet, between young DS and Dahl salt-resistant (DR) rats was seen only in adrenal epinephrine content. DS high-salt (+) rats displayed reduced norepinephrine content relative to DR+ in the mesenteric artery and right atrium. The release of norepinephrine from isolated mesenteric vasculature into the perfusate in response to transmural stimulation showed no significant differences between DS+ and DR+ preparations under basal, or deoxycorticosterone acetate (DOCA; 30 microM) perfusion conditions. The addition of 5 microM cocaine to the DOCA perfusion, while increasing total norepinephrine outflow in all preparations, failed to differentiate between DS+ and DR+. NPY immunofluorescence along mesenteric artery sections of DS+ and DR+ rats was not significantly different. Thus, in the tissues examined, enhanced responsiveness of vascular smooth muscle may not be explained by hypernoradrenergic innervation, elevated NPY innervation or altered release of transmitter.
Kong
J Q
JQ
Department of Pharmacology and Toxicology, West Virginia University, Health Sciences Center, Morgantown.
Curto
K A
KA
Fleming
W W
WW
Kotchen
T A
TA
Taylor
D A
DA
D000818:Animals; D001794:Blood Pressure; D001835:Body Weight; D003042:Cocaine / Q000494:pharmacology; D003900:Desoxycorticosterone / Q000494:pharmacology; D004039:Diet, Sodium-Restricted; D004351:Drug Resistance; D004558:Electric Stimulation; D008297:Male; D008638:Mesenteric Arteries / Q000294:innervation; D009424:Nervous System Physiological Phenomena; D009478:Neuropeptide Y / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism*; D010477:Perfusion; D011312:Pressure; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors
D009478:Neuropeptide Y; D003900:Desoxycorticosterone; D012965:Sodium Chloride; D003042:Cocaine; D009638:Norepinephrine