Oligotyping of celiac multiplex families with the 11th International Histocompatibility Workshop reagents.
Using PCR and SSO probes from the 11th International Histocompatibility Workshop, we oligotyped for HLA-DRB1 gene and DQA1*0501, DQB1*0201 alleles 10 celiac families each with 2 affected children. All families belong to the Italian population except for one, whose mother is originally from Cape Verde island. 8/10 sibling pairs share the DQA1*0501/B1*0201 heterodimer, inherited in cis or in trans arrangement. All the dimer-negative patients were DR4-positive.
Petronzelli
F
F
Department of Experimental Medicine, University La Sapienza, Rome, Italy.
Ferrante
P
P
Triglione
P
P
Bonamico
M
M
Mazzilli
M C
MC
D000328:Adult; D002446:Celiac Disease / Q000235:genetics* / Q000276:immunology; D002648:Child; D004198:Disease Susceptibility / Q000276:immunology; D005260:Female; D005802:Genes, MHC Class II; D020022:Genetic Predisposition to Disease; D006684:HLA-DR Antigens / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D007558:Italy; D008297:Male; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction
D006684:HLA-DR Antigens; D015345:Oligonucleotide Probes