Generation of a monoclonal antibody against avian small dermatan sulfate proteoglycan: immunolocalization and tissue distribution of PG-II (decorin) in embryonic tissues.
Chick embryonic skeletal muscle synthesizes three major types of proteoglycans: large chondroitin sulfate proteoglycans, small dermatan sulfate proteoglycans and small heparan sulfate proteoglycans. A monoclonal antibody has been raised which recognizes the small dermatan sulfate proteoglycan. Immunoblot analysis of a partially purified preparation of skeletal muscle proteoglycans indicates that the antibody reacts with a molecule which migrates with an estimated Mr of 100,000. Prior treatment of the proteoglycans with chondroitinase results in immunostaining of a species of estimated Mr 45,000. These values for the intact proteoglycan and its core protein suggest that the antibody is directed against a proteoglycan of the PG-II or decorin class. Immunohistochemistry indicates a widespread distribution of the proteoglycan, which is localized in connective tissue septa of skeletal and cardiac muscle, dermis, tendon, bone, perichondrium and cornea. Immunoblot analysis of the proteoglycan core proteins from these tissues demonstrates that the antibody recognizes the same 45,000-dalton band in each tissue. The widespread tissue distribution is also consistent with the antibody being directed against an epitope of PG-II. Neither the glycosaminoglycan chains nor N-linked oligosaccharides are required for reactivity and the antibody cross-reacts with other avian material, but not mammalian. This antibody, which has been designated CB-1, reveals developmental stage-specific changes in the deposition of PG-II in embryonic limb bud and skeletal muscle.
Lennon
D P
DP
Department of Biology, Case Western Reserve University, Cleveland, OH 44106.
Carrino
D A
DA
Baber
M A
MA
Caplan
A I
AI
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D002642:Chick Embryo; D002645:Chickens / Q000276:immunology*; D002808:Chondroitin Lyases / Q000494:pharmacology; D011508:Chondroitin Sulfate Proteoglycans / Q000276:immunology* / Q000302:isolation & purification; D003238:Connective Tissue / Q000737:chemistry / Q000196:embryology; D058575:Decorin; D003871:Dermatan Sulfate / Q000276:immunology* / Q000302:isolation & purification; D016326:Extracellular Matrix Proteins; D005455:Fluorescent Antibody Technique; D006801:Humans; D008322:Mammals / Q000276:immunology; D051379:Mice; D009132:Muscles / Q000737:chemistry / Q000196:embryology; D009928:Organ Specificity; D011509:Proteoglycans / Q000032:analysis*
D000911:Antibodies, Monoclonal; D011508:Chondroitin Sulfate Proteoglycans; C546878:DCN protein, human; C546879:Dcn protein, mouse; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; C032649:dermatan sulfate proteoglycan; D003871:Dermatan Sulfate; D002808:Chondroitin Lyases