Effects of the serine analogues isoserine and serinol on interleukin-2 synthesis and phospholipid metabolism in a human T cell line Jurkat.
Phosphatidylserine has been implicated both in the regulation of protein kinase C activity and in the regulation of T lymphocyte activation. Taking into account the fact that some serine analogues modify the activity of the base exchange enzyme system responsible for the synthesis of phosphatidylserine and to a lesser extent the synthesis of phosphatidylcholine and phosphatidylethanolamine, in vitro, we have tested the ability of both isoserine and serinol to modify phospholipid synthesis in the T cell line Jurkat. It was found that serinol was able to decrease by 75% the amount of phosphatidylserine synthesized by the cells and also to decrease the synthesis of phosphatidylcholine and phosphatidylethanolamine, while phosphatidylinositol synthesis was not affected. Concomitantly, in serinol-treated Jurkat cells, interleukin-2 production was markedly inhibited. Monitoring the production of second messengers generated by T cell activators showed that in serinol-treated cells the production of diacylglycerol was impaired while Ca2+ mobilization remained unaffected. Serinol thus appeared to be a potential immunoregulatory molecule active at the level of protein kinase C regulation either through its interaction with phosphatidylserine or through diacylglycerol production.
Pelassy
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C
Unité de Recherches en Immunologie Cellulaire et Moléculaire, INSERM U210, Faculté de Médecine (Pasteur), Nice, France.
Mary
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D
Aussel
C
C
D002118:Calcium / Q000097:blood; D002455:Cell Division; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D004075:Diglycerides / Q000096:biosynthesis; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000187:drug effects*; D010743:Phospholipids / Q000096:biosynthesis / Q000097:blood*; D011412:Propanolamines; D011409:Propylene Glycols / Q000494:pharmacology* / Q000633:toxicity; D015290:Second Messenger Systems / Q000187:drug effects; D012694:Serine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000633:toxicity; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism
D004075:Diglycerides; D007376:Interleukin-2; D010743:Phospholipids; D011412:Propanolamines; D011409:Propylene Glycols; D012694:Serine; C072421:isoserine; C072422:serinol; D002118:Calcium