Isolation and characterization of bacteriophage FC3-10 from Klebsiella spp.
FC3-10 is a Klebsiella spp. specific bacteriophage isolated on a rough mutant (strain KT707, chemotype Rd) of K. pneumoniae C3. The bacteriophage receptor for this phage was shown to be the low-molecular mass lipopolysaccharide (LPS) fraction (LPS-core oligosaccharides), specifically the heptose content of the LPS inner-core. This is the first phage isolated on Klebsiella, the receptor for which is the LPS-core. This phage was unable to plate on Salmonella typhimurium LPS mutants with chemotypes Rd2 or Re showing incomplete or no heptose content on their LPS-core, respectively. Spontaneous phage-resistant mutants from different Klebsiella strains were deep-rough LPS mutants or encapsulated revertants from unencapsulated mutant strains.
Camprubí
S
S
Departamento de Microbiología, Universidad de Barcelona, Spain.
Merino
S
S
Benedí
V J
VJ
Tomás
J M
JM
D000954:Antigens, Surface / Q000378:metabolism; D001435:Bacteriophages / Q000302:isolation & purification* / Q000378:metabolism / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D007709:Klebsiella; D008070:Lipopolysaccharides / Q000378:metabolism; D008854:Microscopy, Electron; D011991:Receptors, Virus / Q000378:metabolism; D014775:Virus Activation
D000954:Antigens, Surface; D008070:Lipopolysaccharides; D011991:Receptors, Virus