Analysis of the gene for phenylalanine ammonia-lyase from Rhodosporidium toruloides.
We have cloned and sequenced the pal gene encoding phenylalanine ammonia-lyase (PAL) from Rhodosporidium toruloides strain CBS14. Our data imply a different start codon and thus a different amino acid sequence for the N-terminus of PAL as compared to the previously published sequence for pal from R. toruloides strain IF00559. Primer extension analysis shows three transcription initiation sites with non-translated leaders of 24-35 nucleotides. Upstream of these initiation sites is a long stretch rich in pyrimidines. PAL from R. toruloides is 78% and 37% homologous to PAL from Rhodotorula rubra and Petroselinum crispum, respectively. Alignment of the PAL sequences is related to data of enzyme function.
Rasmussen
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Genetic Engineering Group, Lyngby, Denmark.
Oerum
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D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014587:Ustilaginales / Q000201:enzymology / Q000235:genetics*
D004271:DNA, Fungal; D010650:Phenylalanine Ammonia-Lyase