[Alterations in insulin receptor in injured rats and the effects of dexamethasone and silybin].
The purpose of this study is to examine the alterations in hepatocyte insulin receptors in rats subjected to scald and restrained water immersion stress and the effects of dexamethasone and silybin. In scalded rats, plasma glucose and insulin levels were higher than that of normal rats, suggesting the occurrence of insulin resistance following injury. The maximal binding capacity (Ro) and highest insulin binding rate (Bo/T) of hepatocyte insulin receptors were significantly reduced in rats 72h postscalding, while there was no obvious change in affinity (Ke Kf). The Ro and Bo/T of the restrained water immersion stress rats were also reduced significantly, but the affinity was increased. Dexamethasone significantly enhanced Ro in rats 72h postscalding with significant decrease in affinity (Ke). Silybin significantly enhanced Ro and Bo/T 72h postscalding with no obvious change in affinity. It is suggested that insulin resistance following injury may be a consequence of receptor defect of the target cells, and for disturbance of glucose metabolism following injury, it is not beneficial to use dexamethasone, but silybin may be used with benefit.
Tang
Z J
ZJ
Department of Pharmacology, Second Military, Medical College, Shanghai.
D000818:Animals; D002056:Burns / Q000188:drug therapy* / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology / Q000627:therapeutic use*; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011972:Receptor, Insulin / Q000187:drug effects* / Q000378:metabolism; D012149:Restraint, Physical; D012838:Silymarin / Q000494:pharmacology / Q000627:therapeutic use*; D013315:Stress, Psychological / Q000378:metabolism
D012838:Silymarin; D003907:Dexamethasone; D011972:Receptor, Insulin