title: Comparison of antiinflammatory and antiallergic drugs in the melittin- and D49 PLA2-induced mouse paw edema models.
abstract: Melittin (MLT) (10 micrograms/paw) and D49 (0.4 micrograms/paw) were injected into the hind paw of male CD-1 mice and elicited 70-80% of maximal paw edema responses at 60 and 30 min after injection, respectively. D49 paw edema was significantly inhibited by anti-histamine/serotonin agents, a PAF antagonist, a PLA2 inhibitor, and some but not all 5-LO and CO inhibitors, indicating that this edema is produced by several classes of inflammatory mediators with mast cell degranulation apparently playing a major role. In contrast, MLT paw edema was not inhibited effectively using the same pharmacological agents except theophylline, suggesting it was elicited via a different sequence of inflammatory events. In summary, D49 and MLT paw edema models were found to be ineffective models to identify experimental PLA2 compounds in our laboratory.
authors.affiliation: Department of Experimental Therapeutics Wyeth-Ayerst Research, Princeton, NJ 08543-8000.
authors.forename: D A
authors.initials: DA
authors.lastname: Hartman
authors.forename: L A
authors.initials: LA
authors.lastname: Tomchek
authors.forename: J R
authors.initials: JR
authors.lastname: Lugay
authors.forename: A C
authors.initials: AC
authors.lastname: Lewin
authors.forename: T T
authors.initials: TT
authors.lastname: Chau
authors.forename: R P
authors.initials: RP
authors.lastname: Carlson
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D003594:Cytoplasmic Granules / Q000187:drug effects; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D005528:Foot; D006633:Histamine Antagonists / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D008407:Mast Cells / Q000187:drug effects; D008555:Melitten; D051379:Mice; D010741:Phospholipases A; D054467:Phospholipases A2
chemical_list: D000893:Anti-Inflammatory Agents; D006633:Histamine Antagonists; D008555:Melitten; D010741:Phospholipases A; D054467:Phospholipases A2