Atherosclerosis and acetaldehyde metabolism in blood.
Acetaldehyde elimination in blood homogenates and erythrocyte aldehyde dehydrogenase (ALDH) activity were studied in 64 patients operated before the age of 60 years because of symptomatic stenosis of aorta, iliac, or carotid arteries and in 38 healthy controls. The disappearance of acetaldehyde in blood homogenates was biphasic. Patients showed an enhanced elimination of acetaldehyde during the second phase (30-60 min), as compared to controls (T1/2 of acetaldehyde was 103 +/- 47 and 198 +/- 93 min, respectively, P less than 0.001). No correlation was found between ALDH activity and acetaldehyde elimination rate. Acetaldehyde elimination in blood homogenates and [14C]acetaldehyde binding to plasma proteins, hemoglobin, and erythrocyte membranes were studied in 10 patients with atherosclerotic disease and in 12 healthy controls. There was a significant correlation between unstable binding of [14C]acetaldehyde to plasma proteins and the half-life of acetaldehyde in the elimination test (p = 0.74, P less than 0.005). Fractionation of plasma proteins after incubation with [14C]acetaldehyde revealed no difference between patients and controls in the distribution of radioactivity. The binding of [14C]acetaldehyde to hemoglobin or erythrocyte membranes did not differ between patients and controls. These results indicate that patients with angiopathy and an enhanced acetaldehyde elimination in blood have reduced binding of acetaldehyde to plasma proteins. As unstable binding of acetaldehyde to proteins is known to involve free amino groups of amino acid residues, modification of these residues in atherosclerotic disease is conceivable.
Ohlin
H
H
Department of Medicine, University Hospital, Lund, Sweden.
Brattström
L
L
Israelsson
B
B
Bergqvist
D
D
Jerntorp
P
P
D000079:Acetaldehyde / Q000097:blood*; D000444:Aldehyde Dehydrogenase / Q000097:blood*; D001024:Aortic Valve Stenosis / Q000097:blood / Q000601:surgery; D001157:Arterial Occlusive Diseases / Q000097:blood / Q000601:surgery; D001161:Arteriosclerosis / Q000097:blood* / Q000201:enzymology / Q000601:surgery; D001798:Blood Proteins / Q000378:metabolism; D016893:Carotid Stenosis / Q000097:blood / Q000601:surgery; D002784:Cholesterol / Q000097:blood; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D006207:Half-Life; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D011485:Protein Binding; D012016:Reference Values; D014280:Triglycerides / Q000097:blood
D001798:Blood Proteins; D006454:Hemoglobins; D014280:Triglycerides; D002784:Cholesterol; D000444:Aldehyde Dehydrogenase; D000079:Acetaldehyde