The role of propylene glycol metabolism in lactatemia in the rabbit.
Propylene glycol (1,2-propanediol PD) has been reported to significantly alter the blood parameters when administered as a drug vehicle. In this study, experiments were performed to estimate the pH, levels of PD, and its metabolites to determine the acute effect of PD in blood. PD was administered to rabbits orally in a single dose of 1 ml 28.4% aqueous solution per 100 g body weight equivalent to 38.66 mmol/kg. Whole blood pH and the levels of PD and metabolites were estimated at fast (O.O h, before feeding PD) and at 0.25, 1, and 3 h after the dose. PD elevated the concentrations of blood PD to its maximum (41.04 +/- 9.98 mmol/liter, n = 4) at 1 h; whereas blood PD is normally absent during fasting. PD significantly increased (P less than 0.01) the concentration of L-lactate in blood, which reached its plateau (2.55 +/- 0.62 mmol/liter, n = 4) at 0.25 h and was 2.45-fold higher than the observed fasted values (1.04 +/- 0.22 mmol/liter, n = 4). Production of D-lactate in blood was similarly increased significantly from 5.1 +/- 5.0 mumols/liter at fast to 150.0 +/- 30.4 mumols/liter at 3 h after oral PD (P less than 0.001, n = 4). As was observed in the fasted blood of PD treated rabbits, D-lactate levels at fast and after saline ingestion in the control animals was found either absent or too low. Despite this increase in lactate, blood pH did not alter significantly when appropriate anticoagulant, i.e., heparin + 4-methylpyrazole, was employed.(ABSTRACT TRUNCATED AT 250 WORDS)
Morshed
K M
KM
INSERM U. 290, Paris, France.
L'Helgoualch
A
A
Nagpaul
J P
JP
Amma
M K
MK
Desjeux
J F
JF
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D004347:Drug Interactions; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007773:Lactates / Q000097:blood*; D019344:Lactic Acid; D014677:Pharmaceutical Vehicles / Q000378:metabolism / Q000494:pharmacology*; D019946:Propylene Glycol; D011409:Propylene Glycols / Q000097:blood / Q000494:pharmacology*; D011817:Rabbits; D013237:Stereoisomerism
D000925:Anticoagulants; D007773:Lactates; D014677:Pharmaceutical Vehicles; D011409:Propylene Glycols; D019344:Lactic Acid; D019946:Propylene Glycol