Micro-quantitative determination of ciprostene in plasma by gas chromatography-mass spectrometry coupled with an antibody extraction.
A simple and highly sensitive method has been developed for the determination in plasma of ciprostene, 9 beta-methyl-6 alpha-carbaprostaglandin I2, using gas chromatography-mass spectrometry following solid-phase extraction on an immobilized antibody column. The anti-ciprostene antibody obtained from rabbit serum was coupled to an agarose support matrix, and the immobilized antibody thus prepared was used as an extraction phase for sample clean-up. The extracted drug was treated with pentafluorobenzyl bromide followed by bis(trimethylsilyl)trifluoroacetamide. The derivative was quantitatively analysed by negative-ion chemical ionization gas chromatography-mass spectrometry. The lower limit of quantitation was 50 pg/ml when 1 ml of human plasma was used. The plasma concentration of ciprostene in a dog treated with ciprostene at 2.5 micrograms/kg was determined successfully by this method.
Komatsu
S
S
Drug Metabolism and Analytical Chemistry Research, Upjohn Pharmaceuticals Limited, Ibaraki, Japan.
Murata
S
S
Aoyama
H
H
Zenki
T
T
Ozawa
N
N
Tateishi
M
M
Vrbanac
J J
JJ
D000818:Animals; D000906:Antibodies; D002846:Chromatography, Affinity; D004285:Dogs; D011464:Epoprostenol / Q000031:analogs & derivatives* / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007477:Ions; D011465:Prostaglandins, Synthetic / Q000097:blood*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000378:metabolism
D000906:Antibodies; D007477:Ions; D011465:Prostaglandins, Synthetic; D012710:Serum Albumin, Bovine; D011464:Epoprostenol; C045329:ciprostene