The role of the O antigen in adjuvant activity of lipopolysaccharide.
Adjuvant activities of isogenic Salmonella enterica, serovar Typhimurium, O-6,7 and O-4,5,12 lipopolysaccharide (LPS), lipid A and Bordetella pertussis LPS were compared by immunizing groups of mice subcutaneously with diphtheria and tetanus toxoid vaccine alone or mixed with one of the LPS derivatives. Five weeks later the mice were bled and the tetanus and diphtheria antibodies in the sera were measured. All the LPS derivatives efficiently increased the antibody responses when compared to the vaccine alone, but the mannose-rich O-6,7 LPS and lipid A were significantly more potent than O-4,5,12 LPS and B. pertussis LPS. We conclude that the quality of the O antigen influences the adjuvant activity of LPS.
Nurminen
M
M
National Public Health Institute, Helsinki, Finland.
Olander
R M
RM
D000818:Animals; D002240:Carbohydrate Sequence; D004168:Diphtheria Toxoid / Q000276:immunology*; D005260:Female; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D019081:O Antigens; D011105:Polyisoprenyl Phosphate Sugars / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D012475:Salmonella / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology*
D004168:Diphtheria Toxoid; D008070:Lipopolysaccharides; D019081:O Antigens; C062656:O-specific polysaccharide, Salmonella; D011105:Polyisoprenyl Phosphate Sugars; D013745:Tetanus Toxoid