title: The uaY positive control gene of Aspergillus nidulans: fine structure, isolation of constitutive mutants and reversion patterns.
abstract: The product of the uaY gene of Aspergillus nidulans is necessary for the expression of at least eight genes coding for enzymes and permeases of the purine utilisation pathway. A detailed fine structure map has been constructed of this gene involving 13 presumed point mutations and eight deletions. Gene conversion of these deletions was demonstrated. A technique was devised to select for constitutive mutations and two were obtained which map within the uaY gene. We have shown that the most centromere proximal allele reverts to a number of different phenotypes. The properties of this allele suggest that it may map in the open reading frame of the uaY gene, in a domain that could be altered in a way that would differentially affect the expression of genes under uaY control.
authors.affiliation: Institut de Génétique et Microbiologie, Unité Associé au CNRS 1354, Université Paris-Sud, Orsay, France.
authors.forename: T
authors.initials: T
authors.lastname: Suárez
authors.forename: N
authors.initials: N
authors.lastname: Oestreicher
authors.forename: J
authors.initials: J
authors.lastname: Kelly
authors.forename: G
authors.initials: G
authors.lastname: Ong
authors.forename: T
authors.initials: T
authors.lastname: Sankarsingh
authors.forename: C
authors.initials: C
authors.lastname: Scazzocchio
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D005785:Gene Conversion; D015966:Gene Expression Regulation, Fungal; D005799:Genes, Dominant; D005800:Genes, Fungal; D005809:Genes, Regulator; D005819:Genetic Markers; D009154:Mutation; D011687:Purines / Q000378:metabolism*
chemical_list: D005819:Genetic Markers; D011687:Purines