title: Comparison and modification of rRNA sequencing methods.
abstract: In this study, modification of two methods of RNA sequencing resulted in more definitive sequencing bands. In one method of sequencing, the bands of lane A and lane G sometimes were not clear. Modifications of this method by changing the concentrations of ddATP and ddGTP resulted in the bands of lane A and lane G becoming more readable. Although a second sequencing method was found to have clearer bands than the first method, and the bases immediately downstream from the primer binding site could be read by using r-32P-labeled primer, the bands on the top of lane A still were not clear. Modifications of this second method by changing the ddATP/dATP ratio resulted in the bands of lane A becoming much clearer.
authors.affiliation: Department of Animal and Poultry Sciences, University of Arkansas, Fayetteville 72701.
authors.forename: R F
authors.initials: RF
authors.lastname: Wang
authors.forename: W W
authors.initials: WW
authors.lastname: Cao
authors.forename: M F
authors.initials: MF
authors.lastname: Slavik
mesh_terms: D001483:Base Sequence; D016123:Campylobacter jejuni / Q000235:genetics; D004247:DNA; D005821:Genetic Techniques; D008969:Molecular Sequence Data; D012335:RNA, Ribosomal / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D004247:DNA