title: [The effect of ethanol on increased expression of HLA-A,-B antigens on lymphocytes].
abstract: Ethanol in a concentration of 0.001% to 2% increases the expressivity of HLA-A, B antigens on lymphocytes. In 26.13% of the examinations HLA antigens were proved only on lymphocytes processed with ethanol. The expressivity is maximally enhanced already by short-term incubation of lymphocytes with ethanol; if prolonged to 120 mins at 20 degrees C ethanol does not produce an unspecific positivity of results. There is no difference between processing of lymphocytes at 20 degrees C and 37 degrees C. 1% and 2% ethanol facilitates detection of HLA-A, B antigens after addition to serum and lymphocytes in the first stage of the microlymphocytotoxic test.
authors.affiliation: Ustav hematologie a krevní transfúze, Praha.
authors.forename: A
authors.initials: A
authors.lastname: Májský
mesh_terms: D000431:Ethanol / Q000494:pharmacology*; D015234:HLA-A Antigens / Q000032:analysis*; D015235:HLA-B Antigens / Q000032:analysis*; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*
chemical_list: D015234:HLA-A Antigens; D015235:HLA-B Antigens; D000431:Ethanol
vernacular_title: Zvýsení expresivity HLA-A, B antigenů na lymfocytech etanolem.