title: Changes in glomerular extracellular matrices components in diabetic nephropathy.
abstract: The changes in glomerular extracellular matrices components in diabetic nephropathy were investigated. Indirect immunofluorescence staining, using polyclonal antibodies to heparan sulfate proteoglycan (HS-PG), laminin, type IV collagen, and fibronectin was carried out on renal specimens obtained by needle biopsy. Immunofluorescence intensity and distribution were observed. HS-PG and laminin decreased in the capillary walls; on the other hand, type IV collagen and fibronectin tended to increase in the mesangial area. HS-PG and laminin decreased in inverse proportion to sclerosis grades and proteinuria. These changes seemed to play an important role in progression of diabetic glomerulosclerosis.
authors.affiliation: Third Department of Internal Medicine, Kyushu University Medical School, Okayama, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ikeda
authors.forename: H
authors.initials: H
authors.lastname: Makino
authors.forename: T
authors.initials: T
authors.lastname: Haramoto
authors.forename: K
authors.initials: K
authors.lastname: Shikata
authors.forename: I
authors.initials: I
authors.lastname: Kumagai
authors.forename: Z
authors.initials: Z
authors.lastname: Ota
mesh_terms: D001707:Biopsy, Needle; D003094:Collagen / Q000032:analysis; D003922:Diabetes Mellitus, Type 1 / Q000473:pathology*; D003924:Diabetes Mellitus, Type 2 / Q000473:pathology*; D003928:Diabetic Nephropathies / Q000473:pathology*; D005109:Extracellular Matrix / Q000648:ultrastructure*; D005260:Female; D005353:Fibronectins / Q000032:analysis; D005455:Fluorescent Antibody Technique; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000032:analysis; D006801:Humans; D007678:Kidney Glomerulus / Q000473:pathology*; D007797:Laminin / Q000032:analysis; D008297:Male; D008875:Middle Aged; D011509:Proteoglycans / Q000032:analysis
chemical_list: D003094:Collagen; D005353:Fibronectins; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D007797:Laminin; D011509:Proteoglycans