Augmentation of macrophage recognition of oxidatively damaged erythrocytes by substratum-bound fibronectin and macrophage surface fibronectin.
Thioglycollate-induced mouse peritoneal macrophages plated on a coverglass bind oxidized mouse erythrocytes in the absence of serum. Macrophages plated on a coverglass pre-coated with fibronectin (FN) were more active in binding of the oxidized erythrocytes. This effect of FN-coated coverglass was due to specific binding of an RGD-containing sequence of FN to FN-receptors on the macrophage, since GRGDSP hexapeptide in solution inhibited this effect, and GRGDSP-coated coverglass exhibited the same effect as FN-coated coverglass. Removal of FN originally present on the macrophage surface by trypsinization, prior to attachment to the coverglass, resulted in diminution of their ability of recognition of the oxidized erythrocytes, but the diminished ability was restored when the trypsinized macrophages were plated on a FN-coated coverglass, indicating that the cell surface FN is required for the macrophage recognition. Attachment to the coverglass was necessary for the cell surface FN to be effective. These results suggest that solid-phase FN, produced either by deposition of soluble FN to substratum or attachment of macrophage surface FN to substratum, activates the macrophages and augments their ability to recognize the oxidized erythrocytes.
Beppu
M
M
Tokyo College of Pharmacy, Japan.
Masa
H
H
Hora
M
M
Kikugawa
K
K
D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D004912:Erythrocytes / Q000502:physiology*; D005353:Fibronectins / Q000494:pharmacology* / Q000502:physiology; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects; D014357:Trypsin / Q000494:pharmacology
D005353:Fibronectins; D009842:Oligopeptides; D014357:Trypsin