Transplantation of beta cells from transgenic mice into nude athymic diabetic rats restores glucose regulation.
We have shown that elevated plasma D-glucose levels in experimentally-induced diabetic nude athymic rats can be reduced by intraperitoneal transplantation of microcarrier-attached insulin producing beta cells from the mouse pancreatic beta cell line, beta TC-1. The reduction in the level of hyperglycemia was observed as early as two days following cell transplantation and was associated with a concomitant increase in plasma insulin levels. beta TC-1 cell transplanted diabetic rats had plasma D-glucose levels similar to those found in non-diabetic control animals and remained normoglycemic throughout the 39 day experimental period. The beta TC-1 cell transplanted diabetic rats also had near normalization of body weight, food and water intake and of urine output when compared to control diabetic and non-diabetic rats. Similarly, they exhibited improved blood glucose clearance following intravenous D-glucose administration. These results suggest that beta TC-1 cells regulate D-glucose homeostasis following transplantation into diabetic rat recipients in a manner similar to that of endogenous pancreatic beta cells.
Hicks
B A
BA
Department of Surgery, Vanderbilt University School of Medicine, Nashville, Tennessee.
Stein
R
R
Efrat
S
S
Grant
S
S
Hanahan
D
D
Demetriou
A A
AA
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002460:Cell Line; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000601:surgery*; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism; D000078790:Insulin Secretion; D016381:Islets of Langerhans Transplantation / Q000473:pathology / Q000502:physiology*; D007700:Kinetics; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic
D001786:Blood Glucose; D007328:Insulin; D005947:Glucose