Induction of ornithine decarboxylase by sialagogues in a human parotid gland adenocarcinoma cell line.
Ornithine decarboxylase in a human parotid gland adenocarcinoma cell line was induced by both cholinergic (carbachol) and beta-adrenergic (isoproterenol) sialagogues. The enzyme protein level, measured with anti-peptide antiserum, as well as the enzyme activity, was found to be high in unstimulated cells and to increase approximately 2-fold on stimulation, while the mRNA level increased 3-4 fold, as revealed by Northern hybridization. The rise in activity was completely blocked by the simultaneous addition of antagonists or actinomycin D. These results suggest that receptor-mediated stimulation of ornithine decarboxylase activity by sialagogues involves alterations in the level of mRNA and that the proliferative responses of human parotid cells to these sialagogues resemble those of the murine parotid gland.
Ueno
A
A
Department of Biochemistry, School of Dentistry, University of Tokushima, Japan.
Kawano
M
M
Ashida
Y
Y
Kinoshita
F
F
Inoue
H
H
D000230:Adenocarcinoma / Q000201:enzymology; D002217:Carbachol / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology*; D009955:Ornithine Decarboxylase / Q000096:biosynthesis* / Q000235:genetics; D010306:Parotid Gland / Q000187:drug effects* / Q000201:enzymology; D010307:Parotid Neoplasms / Q000201:enzymology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology
D012333:RNA, Messenger; D002217:Carbachol; D009955:Ornithine Decarboxylase; D007545:Isoproterenol