[Fermentation micromethod for the quantitative determination of thiamine diphosphate in biological fluids].
An enzymatic micromethod is proposed for quantification of thiamine biphosphate (TBP) at concentrations from 0.5 ng in 0.1-0.2 ml samples of blood or other biological liquids. The dynamics of TBP degradation in blood was studied depending on the time and conditions of storage. A high efficient complex of alcohol dehydrogenase and apopyruvate decarboxylase was isolated from baker's yeasts that can be successfully used for quantitative detection of TBP. The complex was stabilized for further application to biochemical kits for diagnosis of B1-deficiency.
Chernikevich
I P
IP
Gritsenko
E A
EA
Makarchikov
A F
AF
Voskoboev
A I
AI
D000426:Alcohol Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism; D005285:Fermentation; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D009994:Osmolar Concentration; D011767:Pyruvate Decarboxylase / Q000302:isolation & purification / Q000378:metabolism; D011933:Reagent Kits, Diagnostic; D013832:Thiamine Deficiency / Q000175:diagnosis; D013835:Thiamine Pyrophosphate / Q000097:blood*; D015003:Yeasts / Q000201:enzymology
D007527:Isoenzymes; D011933:Reagent Kits, Diagnostic; D000426:Alcohol Dehydrogenase; D011767:Pyruvate Decarboxylase; D013835:Thiamine Pyrophosphate
Fermentativnyĭ mikrometod kolichestvennogo opredeleniia tiamindifosfata v viologicheskikh zhidkostiakh.