Activation of corticotropin-releasing factor-containing neurons in the paraventricular nucleus of the hypothalamus by interleukin-1 in the rat.
Interleukin-1 (IL-1) alpha was injected into the lateral ventricle of the rat. An antiserum against Fos oncoprotein was used to detect the activated neurons immunohistochemically. A large number of neurons in the parvocellular paraventricular nucleus of the hypothalamus, in an area corresponding to the location of the corticotropin-releasing factor (CRF)-containing neurons, were strongly Fos-like immunoreactive (LI). Double immunostaining for Fos and CRF revealed that many of the Fos-LI cells were CRF-LI. Furthermore, the CRF-like immunoreactivity was greatly enhanced in the rats injected with IL-1 alpha, indicating an increase in CRF synthesis.
Ju
G
G
Department of Neurobiology, Fourth Military Medical University, Xian, People's Republic of China.
Zhang
X
X
Jin
B Q
BQ
Huang
C S
CS
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000494:pharmacology*; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D016761:Oncogene Proteins v-fos / Q000378:metabolism; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007375:Interleukin-1; D016761:Oncogene Proteins v-fos; D003346:Corticotropin-Releasing Hormone