Stimulation by carrageenan of arachidonate 12-lipoxygenase activity in dog gingival tissue.
At 4 h after injection of carrageenan into the gingiva, the 12-lipoxygenase activity of the gingival homogenate was markedly increased. Activity in the cytosol and microsomal fractions was markedly increased when assessed as the specific activity based on nmol/min/mg of protein, and in the cytosol fraction as the percentage distribution of total activity. The 12-lipoxygenase activity in the homogenate from carrageenan-treated gingiva was not affected by either EDTA or calcium ion, or a combination of the two. 12-lipoxygenase activity in both carrageenan-treated and untreated gingiva was inhibited dose-dependently by AA861, a striking difference from its effect on platelet 12-lipoxygenase. There was a marked increase of 12-lipoxygenase activity in experimentally inflamed gingiva compared to the non-inflamed gingiva.
Tamai
K
K
Department of Endodontology, Hiroshima University School of Dentistry, Japan.
Dohi
T
T
Yoshino
H
H
Shirakawa
M
M
Okamoto
H
H
Tsujimoto
A
A
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000378:metabolism*; D016718:Arachidonic Acid / Q000378:metabolism; D001792:Blood Platelets / Q000201:enzymology; D002351:Carrageenan / Q000494:pharmacology*; D003600:Cytosol / Q000201:enzymology; D004285:Dogs; D005881:Gingiva / Q000201:enzymology*; D005891:Gingivitis / Q000201:enzymology / Q000473:pathology; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D008861:Microsomes / Q000201:enzymology; D013268:Stimulation, Chemical
D006893:Hydroxyeicosatetraenoic Acids; D016718:Arachidonic Acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D002351:Carrageenan; D001092:Arachidonate 12-Lipoxygenase