New developments in enzymatic peptide synthesis.
This review article describes new enzymatic methods developed for the efficient and irreversible synthesis of peptides based on native and modified proteases, and for the synthesis of polypeptides containing D- and/or unnatural amino acids. Potential opportunities for future developments in the field based on new enzymes, tailor-made catalytic antibodies, and on the technique of in vitro mutagenesis are also described.
Wong
C H
CH
Department of Chemistry, Scripps Research Institute, La Jolla, California 92037.
Wang
K T
KT
D000577:Amides / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry; D002384:Catalysis; D010450:Endopeptidases / Q000737:chemistry; D005544:Forecasting; D007700:Kinetics; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D013237:Stereoisomerism; D013816:Thermodynamics
D000577:Amides; D000596:Amino Acids; D010455:Peptides; D010450:Endopeptidases