title: Positive effect of GAC gene product on the mRNA level of glyoxalase I gene in Saccharomyces cerevisiae.
abstract: A DNA fragment carrying a part of the structural gene for yeast (Saccharomyces cerevisiae) glyoxalase I was cloned from a lambda gt11 expression library using anti-glyoxalase IIgG as a probe. By Northern blotting analysis, the amount of glyoxalase I mRNA was found to increase in yeast cells containing plasmids carrying the GAC gene, which is a positive regulator for yeast glyoxalase I activity. This suggests that the GAC gene product may accelerate the transcription of glyoxalase I gene or may have some positive effects on the accumulation of glyoxalase I mRNA in yeast cells.
authors.affiliation: Research Institute for Food Science, Kyoto University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Inoue
authors.forename: H
authors.initials: H
authors.lastname: Yano
authors.forename: H
authors.initials: H
authors.lastname: Ginya
authors.forename: H
authors.initials: H
authors.lastname: Tsuchiyama
authors.forename: K
authors.initials: K
authors.lastname: Murata
authors.forename: A
authors.initials: A
authors.lastname: Kimura
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D004271:DNA, Fungal; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D005809:Genes, Regulator; D007791:Lactoylglutathione Lyase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids; D012333:RNA, Messenger / Q000378:metabolism; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D012333:RNA, Messenger; D007791:Lactoylglutathione Lyase