Lack of HIV-1 V3 region sequence diversity in two haemophiliac patients infected with a putative biologic clone of HIV-1.
Peripheral blood mononuclear cells (PBMC) from two haemophilia B patients, who presumably became infected with a putative HIV-1 biological clone following treatment with the same suspected batch of commercial factor, were used to clone and sequence the hypervariable V3 region of the HIV-1 envelope protein. In 10 of 12 clones the V3 region was identical and two (one from each patient) had a single non-synonymous point mutation. Viable reisolates (shown to be authentic by sequencing of V3) currently appear to be monocyte tropic. These results strongly indicate that the patients were infected from a common source with a very low number of infectious particles and indicate that variability under these conditions is limited.
Cichutek
K
K
Paul-Ehrlich-Institut, Langen, Germany.
Norley
S
S
Linde
R
R
Kreuz
W
W
Gahr
M
M
Löwer
J
J
von Wangenheim
G
G
Kurth
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001769:Blood / Q000382:microbiology; D002675:Child, Preschool; D005164:Factor IX / Q000009:adverse effects; D014644:Genetic Variation; D015699:HIV Envelope Protein gp120 / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006467:Hemophilia A / Q000150:complications; D006801:Humans; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D015699:HIV Envelope Protein gp120; D005164:Factor IX