title: Structural and phylogenetic analysis of the chicken ventricular myosin heavy chain rod.
abstract: We have isolated and characterized five overlapping clones that encompass 3.2 kb and encode a part of the short subfragment 2, the hinge, and the light meromyosin regions of the myosin heavy chain rod as well as 143 bp of the 3' untranslated portion of the mRNA. Northern blot analysis showed expression of this mRNA mainly in ventricular muscle of the adult chicken heart, with trace levels detected in the atrium. Transient expression was seen in skeletal muscle during development and in regenerating skeletal muscle following freeze injury. To our knowledge, this is the first report of an avian ventricular myosin heavy chain sequence. Phylogenetic analysis indicated that this isoform is a distant homolog of other ventricular and skeletal muscle myosin heavy chains and represents a distinct member of the multigene family of sarcomeric myosin heavy chains. The ventricular myosin heavy chain of the chicken is either paralogous to its counterpart in other vertebrates or has diverged at a significantly higher rate.
authors.affiliation: Department of Organismal Biology and Anatomy, University of Chicago, IL 60637.
authors.forename: A F
authors.initials: AF
authors.lastname: Stewart
authors.forename: B
authors.initials: B
authors.lastname: Camoretti-Mercado
authors.forename: D
authors.initials: D
authors.lastname: Perlman
authors.forename: M
authors.initials: M
authors.lastname: Gupta
authors.forename: S
authors.initials: S
authors.lastname: Jakovcic
authors.forename: R
authors.initials: R
authors.lastname: Zak
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015152:Blotting, Northern; D002645:Chickens / Q000145:classification / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D006352:Heart Ventricles / Q000737:chemistry*; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism; D009218:Myosins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D015183:Restriction Mapping; D016415:Sequence Alignment
chemical_list: D012333:RNA, Messenger; D004247:DNA; D009218:Myosins