title: A highly sensitive chemiluminescent assay for glycerylphosphorylcholine in human seminal plasma.
abstract: Glycerylphosphorylcholine (GPC), one of the major phosphorus-containing-choline compounds of seminal plasma, is secreted mainly by the epididymal epithelium under androgenic control. This study reports a new method that uses chemiluminescence to determine seminal GPC content, comparing it with a spectrophotometric technique. The results, obtained with both techniques studying 20 fertile patients (as control), 35 infertile patients with normospermia, 23 infertile patients with oligozoospermia and impaired motility and 9 patients with excretory azoospermia, demonstrate that the GPC chemiluminescent assay is more sensitive, simple and rapid than the spectrophotometric assay. Our data confirm that GPC may be used as a marker of vas deferens and ejaculatory duct perviousness, suggesting a possible role of this glycerophosphodiester in sperm motility.
authors.affiliation: Clinical Biochemistry Department, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Pacifici
authors.forename: F
authors.initials: F
authors.lastname: Dondero
authors.forename: P
authors.initials: P
authors.lastname: Giovenco
authors.forename: S
authors.initials: S
authors.lastname: Pichini
authors.forename: P
authors.initials: P
authors.lastname: Zuccaro
mesh_terms: D005997:Glycerylphosphorylcholine / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism; D008163:Luminescent Measurements; D008297:Male; D012661:Semen / Q000737:chemistry*; D013057:Spectrum Analysis
chemical_list: D005997:Glycerylphosphorylcholine