Isolation and characterization of liver-specific antigen.
The purpose of this work is to develop a method to determine liver injuries using liver-specific substances. Initially, the liver-specific antigen (LSA) was purified from the human liver. The human LSA found in the Sephadex G-100 gel filtration first peak, has been isolated and characterized from normal human liver water-soluble proteins. Purification of LSA was carried out by consecutive gel filtration, ammonium sulfate precipitation, and anion and cation ion exchange chromatography, while simultaneously monitoring its reactivity using the antibody against the first peak fraction of the human liver extract through Sephadex G-100 after absorption with serum and kidney extract. This antigen was found to have a single band in SDS electrophoresis (PAGE) and the M.W. of approximately 52 KD. By IEF electrophoresis, the isoelectric point of some constituents were found to be pI 5.8-5.9. In addition, the antibody to this antigen was examined for organ specificity using the immunoblotting technique against the human kidney, lung, heart, spleen, pancreas, skeletal muscle, brain extracts and serum, respectively. The immunogenicity and characteristics of this antigen were found to be different from other specific antigens in the liver, which have been previously reported.
Seo
Y
Y
Department of Legal Medicine, Miyazaki Medical College, Japan.
Takahama
K
K
D005554:Forensic Medicine; D006801:Humans; D007519:Isoantigens / Q000302:isolation & purification*; D008099:Liver / Q000293:injuries
D007519:Isoantigens; C030497:liver specific F antigen