title: Increased plasminogen activator activity and plasminogen activator inhibition in spermatozoa and seminal plasma of the ram after serum gonadotrophin (PMSG) administration. Correlation with the increased level of testosterone in the blood.
abstract: After a single injection of serum gonadotrophin (PMSG) at the dose of 15 IU/kg, i.m. into rams testosterone in the plasma of blood showed a significant rise between 4th and 7th day post-injection. At the same time (4th-7th day) the plasminogen activator activity (PAA) in seminal plasma was found to be increased, but the plasminogen activator inhibition (PAI) expressed against t-PA (anti-t-PA) showed an increase between 32nd and 46th day. In spermatozoa a marked increase of PAA was revealed between 32nd and 46th day post-injection, while an increase of PAI (anti-t-PA) was exhibited on the 74th day. Plasmin inhibition (PI) in seminal plasma and spermatozoa showed no change compared to controls. A positive correlation has been found between increased concentrations of testosterone and PAA or PAI (anti-t-PA) in spermatozoa and seminal plasma. The induced increase of PAA in spermatozoa under the effect of testosterone might be of physiological importance, since PAA is localized to sperm membranes and might participate in the whole process of fertilization.
authors.affiliation: Department of Physiology, Faculty of Veterinary Medicine, Aristotelian University of Thessaloniki, Greece.
authors.forename: C
authors.initials: C
authors.lastname: Rekkas
authors.forename: S
authors.initials: S
authors.lastname: Belibasaki
authors.forename: I
authors.initials: I
authors.lastname: Taitzoglou
authors.forename: N
authors.initials: N
authors.lastname: Kokolis
authors.forename: A
authors.initials: A
authors.lastname: Smokovitis
mesh_terms: D000818:Animals; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D008297:Male; D010960:Plasminogen Activators / Q000378:metabolism*; D015849:Plasminogen Inactivators / Q000378:metabolism*; D012016:Reference Values; D012661:Semen / Q000187:drug effects / Q000378:metabolism*; D012756:Sheep; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000097:blood*; D013997:Time Factors; D010959:Tissue Plasminogen Activator / Q000037:antagonists & inhibitors
chemical_list: D006064:Gonadotropins, Equine; D015849:Plasminogen Inactivators; D013739:Testosterone; D010960:Plasminogen Activators; D010959:Tissue Plasminogen Activator