Identification of mycobacteria by high-performance liquid chromatography.
Mycolic acids extracted from saponified mycobacterial cells were examined as p-bromophenacyl esters by high-performance liquid chromatography (HPLC). Standard HPLC patterns were developed for species of Mycobacterium by examination of strains from culture collections and other well-characterized isolates. Relative retention times of peaks and peak height comparisons were used to develop a differentiation scheme that was 98% accurate for the species examined. A rapid, cost-effective HPLC method which offers an alternative approach to the identification of mycobacteria is described.
Butler
W R
WR
Division of Bacterial and Mycotic Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
Jost
K C
KC
Kilburn
J O
JO
D002851:Chromatography, High Pressure Liquid; D005069:Evaluation Studies as Topic; D006801:Humans; D009161:Mycobacterium / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification; D009171:Mycolic Acids / Q000032:analysis*; D013045:Species Specificity
D009171:Mycolic Acids