Cloning and expression of cDNAs from enterically-transmitted non-A, non-B hepatitis virus.
The fragment gene of enterically-transmitted non-A, non-B hepatitis virus (ET-NANBHV) was cloned as a cDNA and inserted into an expression vector pUEX2. The recombinant protein was expressed in Escherichia coli HB101 as a fusion protein with beta-galactosidase (beta-Gal). The fusion protein reacted with the sera of infected cynomolgus monkeys and of patients from Myanmar. This reaction was highly related with ET-NANBHV infection, and obviously demonstrates in that the recombinant protein can be used for the detection of ET-NANBHV infection.
Ichikawa
M
M
Chemo-Sero Therapeutic Research Institute, Kikuchi Laboratories, Kumamoto.
Araki
M
M
Rikihisa
T
T
Uchida
T
T
Shikata
T
T
Mizuno
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D006508:Hepatitis Antibodies / Q000032:analysis; D016751:Hepatitis E / Q000276:immunology*; D016752:Hepatitis E virus / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008252:Macaca fascicularis; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology
D006508:Hepatitis Antibodies; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D004247:DNA