title: Effect of human C-peptide on glucose transport in in vitro incubated human skeletal muscle.
abstract: Muscle specimens from the quadriceps femoris muscle were obtained from eight healthy subjects by means of an open muscle biopsy and prepared for in vitro incubation. C-peptide at 0.5, 1.0 and 2.5 nmol/l increased 3-0-methylglucose transport by 38% (NS), 64% (p less than 0.05), and 64% (p less than 0.05) respectively. Glucose transport increased 1.8-fold in the presence of 0.3 nmol/l of insulin (p less than 0.05). Glycogen content in muscle strips exposed to C-peptide at a concentration of 1 nmol/l increased significantly by 22% (p less than 0.05). In conclusion, C-peptide stimulates the rate of 3-0-methylglucose transport in in vitro incubated human skeletal muscle strips in a dose-response manner. These observations suggest that C-peptide may contribute to the regulation of carbohydrate metabolism in human skeletal muscle.
authors.affiliation: Department of Clinical Physiology, Karolinska Hospital, Stockholm, Sweden.
authors.forename: J R
authors.initials: JR
authors.lastname: Zierath
authors.forename: D
authors.initials: D
authors.lastname: Galuska
authors.forename: B L
authors.initials: BL
authors.lastname: Johansson
authors.forename: H
authors.initials: H
authors.lastname: Wallberg-Henriksson
mesh_terms: D019325:3-O-Methylglucose; D000328:Adult; D001693:Biological Transport, Active / Q000187:drug effects; D002096:C-Peptide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D008757:Methylglucosides / Q000378:metabolism*; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*
chemical_list: D002096:C-Peptide; D007328:Insulin; D008757:Methylglucosides; D019325:3-O-Methylglucose