Determination of cytosine-beta-D-arabinoside in plasma using capillary electrophoresis.
An assay for the antileukaemic agent cytosine-beta-D-arabinoside (ara-C) has been developed using capillary zone electrophoresis. Solid-phase extraction and on-capillary peak concentration are used to improve the detection limit. The electrophoretic separation time is less than 5 min. The limit of detection for ara-C in plasma is 0.5 microM (signal-to-noise ratio = 3). The assay has been validated for the determination of ara-C in human plasma over the concentration range 1-10 microM. The calibration curve was linear with a correlation coefficient r2 = 0.996. At an ara-C concentration of 8 microM the intra-day coefficient of variation was 9.1% and the inter-day coefficient of variation was 12.3%. At an ara-C concentration of 2 microM the coefficients of variation were 15.2 and 12.0%, respectively.
Lloyd
D K
DK
St. Jude Childrens Research Hospital, Pharmacokinetics Section, Memphis, TN 38101-0318.
Cypess
A M
AM
Wainer
I W
IW
D003561:Cytarabine / Q000097:blood*; D004586:Electrophoresis / Q000379:methods*; D006801:Humans
D003561:Cytarabine