title: Structure and sequence analysis of the human activin beta A subunit gene.
abstract: The cloned genomic DNA containing the human activin beta A subunit gene were analyzed by restriction endonuclease mapping, Southern blotting and DNA sequencing. The activin gene is composed of two exons interrupted by the 9-kb intron. The TATA, CCAAT and CT-stretch sequences were found in the 5'-flanking region of the gene. An intronic sequence contained SV40 enhancer core element in the vicinity of the exon 1. In the 3'-flanking region, we identified eight consensus polyadenylation sequences, five ATTTA motifs, CA element consisting of (CA)14, AP-1 binding site and two SV40 enhancer core elements. A dot matrix analysis revealed the high degree of conservation between the human and rat sequences within the 3'-flanking region, suggesting a possible functional significance.
authors.affiliation: Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tanimoto
authors.forename: S
authors.initials: S
authors.lastname: Handa
authors.forename: N
authors.initials: N
authors.lastname: Ueno
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: A
authors.initials: A
authors.lastname: Fukamizu
mesh_terms: D028341:Activins; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA; D005786:Gene Expression Regulation; D006801:Humans; D007265:Inhibins / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D015183:Restriction Mapping
chemical_list: D028341:Activins; D007265:Inhibins; D004247:DNA