High-performance liquid chromatographic determination of rifapentine and its metabolite in human plasma by direct injection into a shielded hydrophobic phase column.
A simple high-performance liquid chromatographic method for determination of rifapentine, a cyclopentyl semisynthetic analogue of rifamycin belonging to the class of piperazinyl hydrazone derivatives of 3-formylrifamycin SV, and its metabolite, 25-desacetylrifapentine, in human plasma was developed using direct injection of the sample onto a Supelco LC HISEP column. The mean recovery was 100.3% for rifapentine and 99.7% for the metabolite and the precision of the assays was 3% and 7%, respectively. The limit of determination was 0.2 micrograms/ml and the method was validated for concentrations up to 64 micrograms/ml for rifapentine and 32 micrograms/ml for the metabolite. The results correlated well with those of the microbiological assay with Sarcina lutea as test organism.
Riva
E
E
Marion Merrell Dow Research Institute, Lepetit Centre, Gerenzano, Italy.
Merati
R
R
Cavenaghi
L
L
D000900:Anti-Bacterial Agents / Q000097:blood* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D006801:Humans; D015203:Reproducibility of Results; D012293:Rifampin / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism / Q000494:pharmacology; D012505:Sarcina / Q000187:drug effects; D013056:Spectrophotometry, Ultraviolet
D000900:Anti-Bacterial Agents; C032006:25-desacetylrifapentine; D012293:Rifampin; C018421:rifapentine