title: Changes in polypeptide expression following Trypanosoma cruzi differentiation from trypomastigotes to amastigotes.
abstract: Changes in the dynamic of expression of polypeptides following the differentiation from infective trypomastigotes to multiplicative amastigote forms of Trypanosoma cruzi were mapped by two-dimensional gel electrophoresis and quantitatively analyzed by laser densitometry. Following the differentiation from trypomastigotes to amastigotes the expression of the polypeptides 212, 183, 176, 149, 50-55, 43, 39, 34 and 28 kDa is turned off in multiplicative amastigotes, whereas the expression of the polypeptides 80, 66 (p.Is. 6.75-7.50), 42 and 38 kDa is turned on. After complete differentiation from trypomastigotes to amastigotes the expression of the polypeptides 43, 42, 33, 32, 29 and 23 kDa is up-regulated in amastigotes, whereas the expression of the acidic polypeptides 66 (p.Is. 6.27-6.64), 45-48 and 41-43 kDa is down-regulated.
authors.affiliation: Division of Biomedical Sciences, Meharry Medical College, Nashville, Tennessee 37208.
authors.forename: A
authors.initials: A
authors.lastname: Ruiz-Ruano
authors.forename: F
authors.initials: F
authors.lastname: Villalta
authors.forename: M F
authors.initials: MF
authors.lastname: Lima
mesh_terms: D000818:Animals; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics; D015800:Protozoan Proteins / Q000096:biosynthesis* / Q000235:genetics; D014349:Trypanosoma cruzi / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014709:Vero Cells
chemical_list: D010455:Peptides; D015800:Protozoan Proteins