Separation of two subpopulations of old human fibroblasts by mitochondria (rhodamine 123) fluorescence.
The mitochondria-binding fluorescent dye rhodamine 123 (R123) was used to sort two subpopulations of cells from mass cultures of old human fibroblasts (HF). Both subpopulations showed unusual dye retention kinetics when compared to young HF. Additionally, electron microscopy revealed differences in numerical density of mitochondria between young and old HF. Moreover, the two subpopulations exhibited differences in radiolabeled thymidine incorporation and proliferative capacity. The results obtained suggest a possible relationship between mitochondria, proliferative potential and cellular aging.
Martinez
A O
AO
Division of Life Sciences, University of Texas, San Antonio 78285.
Over
D
D
Armstrong
L S
LS
Manzano
L
L
Taylor
R
R
Chambers
J
J
D002455:Cell Division; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D004272:DNA, Mitochondrial / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D005434:Flow Cytometry; D005453:Fluorescence; D006801:Humans; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure*; D020112:Rhodamine 123; D012235:Rhodamines; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D004272:DNA, Mitochondrial; D012235:Rhodamines; D014316:Tritium; D020112:Rhodamine 123; D013936:Thymidine