Comparison of an ELISA-based screening test with liquid chromatography for the determination of aflatoxins in corn.
An enzyme-linked immunosorbent assay (ELISA) screening test (CITE PROBE) was compared to liquid chromatography (LC) for the determination of aflatoxins in naturally contaminated corn samples. The CITE PROBE, with a positive/negative cutoff of 5 ng/g aflatoxin B1, was correct (based on LC results) on 47 of 51 samples. Two of the incorrect responses by the CITE PROBE were false positives on samples containing 4.4 ng/g and 4.1 ng/g aflatoxins by LC. Another incorrect response was a false negative on a sample containing 5.5 ng/g aflatoxins by LC. The fourth incorrect response was a false positive on a sample containing 1.9 ng/g aflatoxins by LC. On the basis of these results, the CITE PROBE was determined to be a reliable screening method for the detection of greater than or equal to 5 ng/g aflatoxins in corn.
Beaver
R W
RW
University of Georgia, Department of Plant Pathology, Tifton 31793.
James
M A
MA
Lin
T Y
TY
D000348:Aflatoxins / Q000032:analysis*; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation; D005506:Food Contamination / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry*
D000348:Aflatoxins