Rapid synthesis and introduction of a protected EDTA-like group during the solid-phase assembly of peptides.
A simple two-step procedure is reported for the synthesis of a tert-butyl ester protected form of an EDTA-like bifunctional chelating agent. This reagent can be easily introduced on any available amino group during the assembly of peptides on solid-phase supports. Using the model tetradecapeptide OVA(323-336), we have introduced an EDTA group at the N-terminus of this T-cell epitope and confirmed that the EDTA group is present on the molecule, can chelate metals, and does not affect the biological activity of the peptide.
Arya
R
R
Department of Medical Biophysics, University of Toronto, Ontario, Canada.
Gariépy
J
J
D000941:Antigens / Q000276:immunology; D002614:Chelating Agents / Q000138:chemical synthesis*; D004492:Edetic Acid / Q000737:chemistry* / Q000276:immunology; D007376:Interleukin-2; D008213:Lymphocyte Activation; D010047:Ovalbumin / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000941:Antigens; D002614:Chelating Agents; D007376:Interleukin-2; D010446:Peptide Fragments; D010047:Ovalbumin; D004492:Edetic Acid