title: Antimicrobial effects from incorporation of disinfectants into gypsum casts.
abstract: Five microorganisms--Escherichia coli, Streptococcus mutans, Staphylococcus aureus, Serratia marcescens, and Bacillus subtilis--were placed into the canine, first premolar, first molar, and second molar of sterilized quadrant addition-reaction silicone impressions. Four disinfectant solutions--iodophor, neutral glutaraldehyde, phenol, and sodium hypochlorite--were spatulated with sterilized Type IV stone and vibrated into the intentionally contaminated impressions. The casts were removed at 1 and 24 hours, and the surfaces were swabbed, plated on BHI agar, and incubated at 37 degrees C for 3 days. Colony forming units were counted. At 1 hour, only sodium hypochlorite and glutaraldehyde effectively reduced the number of bacteria to the negative control. Incorporation of sodium hypochlorite, glutaraldehyde, and iodophor into gypsum casts reduced viable bacteria to a level equal to the negative controls at 24 hours.
authors.affiliation: School of Dentistry, University of California, San Francisco.
authors.forename: S M
authors.initials: SM
authors.lastname: Mansfield
authors.forename: J M
authors.initials: JM
authors.lastname: White
mesh_terms: D001420:Bacteria, Aerobic / Q000187:drug effects; D002133:Calcium Sulfate; D015169:Colony Count, Microbial; D004202:Disinfectants; D004866:Equipment Contamination; D005976:Glutaral; D017053:Infection Control / Q000379:methods*; D007466:Iodophors; D007754:Laboratories, Dental; D003765:Models, Dental; D010636:Phenols; D012973:Sodium Hypochlorite; D013997:Time Factors
chemical_list: D004202:Disinfectants; D007466:Iodophors; D010636:Phenols; D012973:Sodium Hypochlorite; D005976:Glutaral; D002133:Calcium Sulfate