Interleukin 1 alpha mRNA-expressing cells on the local inflammatory response in feline infectious peritonitis.
By in situ hybridization with biotinylated human interleukin 1 alpha (IL-1 alpha) cDNA probe, distribution of feline IL-1 alpha mRNA-expressing cells was examined in the tissues from 49 cases diagnosed as feline infectious peritonitis (FIP) by pathological examination. IL-1 alpha mRNA-expressing cells were found in visceral peritoneum, lymphoid organs, liver, kidney, pancreas, digestive tract, lung, pleura, brain, palpebral conjunctiva, and bone marrow. Hybridization signals for IL-1 alpha mRNA were mostly located in the local inflammatory lesions on the serosal surface of various organs and omentum, which were frequently involved in the lesions of FIP (27.8 +/- 5.1 cells/mm2). Morphological examination suggested that they were infiltrated macrophages. However, few IL-1 alpha mRNA-expressing macrophages were in the lesions of other organs. These data suggested that IL-1 alpha produced from macrophages in the local inflammatory sites might participate in the initiation and development of the lesions on the visceral peritoneum in FIP.
Hasegawa
T
T
Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.
Hasegawa
A
A
D000818:Animals; D002415:Cats; D015342:DNA Probes; D016766:Feline Infectious Peritonitis / Q000276:immunology* / Q000473:pathology; D005786:Gene Expression Regulation; D007375:Interleukin-1 / Q000032:analysis* / Q000235:genetics; D008099:Liver / Q000737:chemistry / Q000473:pathology; D008643:Mesentery / Q000737:chemistry / Q000473:pathology; D009852:Omentum / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis*; D014781:Viscera / Q000737:chemistry / Q000473:pathology
D015342:DNA Probes; D007375:Interleukin-1; D012333:RNA, Messenger