title: The mast cell and histamine concentration of the human post-menopausal uterus.
abstract: Mast cells and histamine concentrations have been studied in uteri removed by hysterectomy from women in their post-menopausal years. Mast cell numbers were expressed as mean numbers/mm2 following fixation in 10% formalin and staining with Azure B. The majority of mast cells, in both the endometrium and myometrium, were very densely stained. Mast cells in the myometrium showed a significant negative correlation with years post-menopausal (rs = -0.52, P less than 0.05). Extracted histamine from uterine tissue was condensed with o-phthaldialdehyde to form a fluorophore and its fluorescence was measured at 450 microns using a spectrofluorometer. No significant correlation was found between histamine concentrations in the uterine wall and years post-menopausal.
authors.affiliation: TCD Department of Obstetrics and Gynaecology, Sir Patrick Dun Research Centre, St. James's Hospital, Dublin, Ireland.
authors.forename: L
authors.initials: L
authors.lastname: Drudy
authors.forename: B L
authors.initials: BL
authors.lastname: Sheppard
authors.forename: J
authors.initials: J
authors.lastname: Bonnar
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002452:Cell Count; D004717:Endometrium / Q000166:cytology / Q000378:metabolism; D005260:Female; D006632:Histamine / Q000096:biosynthesis*; D006801:Humans; D008407:Mast Cells / Q000166:cytology*; D008875:Middle Aged; D009215:Myometrium / Q000166:cytology / Q000378:metabolism; D014599:Uterus / Q000166:cytology / Q000378:metabolism*
chemical_list: D006632:Histamine