title: Two isoforms of the muscle acetylcholine receptor alpha-subunit are translated in the human cell line TE671.
abstract: We have previously reported the existence of 2 forms of mRNA for the human muscle acetylcholine receptor (AChR) alpha-subunit, thought to be generated by alternate splicing of a primary transcript and to encode 2 alpha-subunit protein isoforms. The 2 predicted alpha-subunit isoforms, differing by the insertion of 25 amino acids at position 58/59, have been synthesized from cRNA transcripts using rabbit reticulocyte lysates; these protein isoforms could be differentiated by immunoprecipitation using antibodies raised against synthetic peptides. The antibodies were used to demonstrate translation of both AChR alpha-subunit isoforms in the rhabdomyosarcoma (muscle) cell line TE671, in an approximate 1:1 ratio.
authors.affiliation: Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
authors.forename: A
authors.initials: A
authors.lastname: Morris
authors.forename: D
authors.initials: D
authors.lastname: Beeson
authors.forename: L
authors.initials: L
authors.lastname: Jacobson
authors.forename: F
authors.initials: F
authors.lastname: Baggi
authors.forename: A
authors.initials: A
authors.lastname: Vincent
authors.forename: J
authors.initials: J
authors.lastname: Newsom-Davis
mesh_terms: D000595:Amino Acid Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D005091:Exons; D006801:Humans; D007106:Immune Sera; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D014176:Protein Biosynthesis; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D011950:Receptors, Cholinergic / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D015183:Restriction Mapping
chemical_list: D007106:Immune Sera; D046911:Macromolecular Substances; D010455:Peptides; D012333:RNA, Messenger; D011950:Receptors, Cholinergic