[Quantitative characteristics and reconstruction of the surface topography of histochemical markers on the nerve cell plasmalemma].
An electron microscopic study of topography of colloidal gold-bound lectins conjugating to glycocalix carbohydrate residues on the somatic membrane surface of the cultivated spinal neurons has been carried out. The quantitative procedures are suggested for analyzing the surface pattern of the markers: two stochastic functions are considered to correspond properly to the particle distribution observed in the electron micrograms. The analysis of these functions permits obtaining required numerical characteristics. The Monte Carlo reconstructing model is described, and results of its work (on the basis of the above experimental data) are demonstrated in the form of "averaged" surface topography of the studied markers within the membrane fragments bordered. Possible connection of the obtained data with cooperative properties of the membrane is discussed.
Rusakov
D A
DA
Skibo
G G
GG
Berezovskaia
O L
OL
Koval'
L M
LM
D000818:Animals; D015415:Biomarkers / Q000737:chemistry; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D006651:Histocytochemistry; D007091:Image Processing, Computer-Assisted; D051379:Mice; D009010:Monte Carlo Method; D009474:Neurons / Q000502:physiology*; D011336:Probability; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology*
D015415:Biomarkers
Chislennye kharakteristiki i rekonstruktsiia poverkhnostnoĭ topografii gistokhimicheskikh markerov na plazmalemme nervnykh kletok.