title: Crystal-neutrophil interactions lead to interleukin-1 synthesis.
abstract: Normal human blood neutrophils were studied for their capacity to synthesize and release interleukin-1 (IL-1) species after phagocytosis of triclinic monosodium urate (MSU) and calcium pyrophosphate dihydrate crystals (CPPD). MSU crystals were more potent inducers of IL-1 generation than CPPD or unopsonized zymosan. Microcrystal-stimulated neutrophils characteristically secreted most of the newly synthesized IL-1. Colchicine partly inhibited the secretion of IL-1 by neutrophils during phagocytosis of solid particles. However, colchicine selectively inhibited IL-1 synthesis induced by microcrystals. These results suggest that neutrophil-derived IL-1 may contribute to the pathogenesis of crystal-induced arthritis.
authors.affiliation: Unité de Recherche Inflammation et Immunologie-Rhumatologie, Centre de Recherche du CHUL, Boul Laurier, Québec, Canada.
authors.forename: C J
authors.initials: CJ
authors.lastname: Roberge
authors.forename: J
authors.initials: J
authors.lastname: Grassi
authors.forename: R
authors.initials: R
authors.lastname: De Médicis
authors.forename: Y
authors.initials: Y
authors.lastname: Frobert
authors.forename: A
authors.initials: A
authors.lastname: Lussier
authors.forename: P H
authors.initials: PH
authors.lastname: Naccache
authors.forename: P E
authors.initials: PE
authors.lastname: Poubelle
mesh_terms: D002131:Calcium Pyrophosphate; D003078:Colchicine / Q000494:pharmacology; D003460:Crystallization; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis*; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010587:Phagocytosis / Q000502:physiology*; D014527:Uric Acid; D015054:Zymosan / Q000494:pharmacology
chemical_list: D007375:Interleukin-1; D014527:Uric Acid; D015054:Zymosan; D003078:Colchicine; D002131:Calcium Pyrophosphate