DNA-binding transferrin conjugates as functional gene-delivery agents: synthesis by linkage of polylysine or ethidium homodimer to the transferrin carbohydrate moiety.
We have previously demonstrated that transferrin-polycation conjugates are efficient carrier molecules for the introduction of genes into eukaryotic cells. We describe here a more specific method for conjugation of transferrin with DNA-binding compounds involving attachment at the transferrin carbohydrate moiety. We used the polycation poly(L-lysine) or the DNA intercalator, ethidium homodimer as DNA-binding domains. Successful transferrin-receptor-mediated delivery and expression of the Photinus pyralis luciferase gene in K562 cells has been shown with these new transferrin conjugates. The activity of the transferrin-ethidium homodimer (TfEtD) conjugates is low relative to transferrin-polylysine conjugates; probably because of incomplete condensation of the DNA. However, DNA delivery with TfEtD is drastically improved when ternary complexes of the DNA with TfEtD and the DNA condensing agent polylysine are prepared. The gene delivery with the carbohydrate-linked transferrin-polylysine conjugates is equal or superior to described conjugates containing disulfide linkage. The new ligation method facilitates the synthesis of large quantities (greater than 100 mg) of conjugates.
Wagner
E
E
Research Institute of Molecular Pathology, Vienna, Austria.
Cotten
M
M
Mechtler
K
K
Kirlappos
H
H
Birnstiel
M L
ML
D002240:Carbohydrate Sequence; D004247:DNA / Q000378:metabolism*; D004996:Ethidium / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D008722:Methods; D008969:Molecular Sequence Data; D011107:Polylysine / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000031:analogs & derivatives; D014162:Transfection; D014168:Transferrin / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000031:analogs & derivatives
D014168:Transferrin; C072482:transferrin-ethidium homodimer conjugate; C072481:transferrin-polylysine conjugate; D011107:Polylysine; C018533:ethidium homodimer; D004247:DNA; D004996:Ethidium