Nucleotide sequence of the 3' nuclease-sensitive region of the human phosphoglycerate kinase 1 (PGK1) gene.
Many genes are known to have nuclease-sensitive sites and/or control sequences in their 3' flanking regions, but for very few genes has this region been sequenced. Previously, we mapped specific, gene activity-dependent DNAase I- and MspI-sensitive sites at the 3' end of the human X-linked housekeeping gene phosphoglycerate kinase (PGK1). Sequence information presented here shows that the 3' nuclease-sensitive site maps precisely to an Alu sequence and near a "BKM" repeat. This is the first report of an Alu sequence that has alternative chromatin configurations depending on gene activity.
Riley
D E
DE
Department of Urology, School of Medicine, University of Washington, Seattle 98195.
Goldman
M A
MA
Gartler
S M
SM
D001483:Base Sequence; D004247:DNA; D016208:Databases, Factual; D003851:Deoxyribonucleases / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010735:Phosphoglycerate Kinase / Q000235:genetics* / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
D004247:DNA; D010735:Phosphoglycerate Kinase; D003851:Deoxyribonucleases