Determination of erythrocytic polyamines by reversed-phase liquid chromatography.
A simple and rapid semiautomated procedure for determining polyamines in erythrocytes by high-performance liquid chromatography is described. Putrescine, spermidine, and spermine are converted to fluorescent dansyl derivatives, extracted with cyclohexane, and separated in less than 10 min on a reversed-phase C18 ODS column, with an acetonitrile-water gradient as the mobile phase. The method showed a coefficient of variation of 2.73% for spermidine and 3.27% for spermine. The respective reference values, evaluated in 10 healthy patients, were 7.88 (SD 2.09) and 5.42 (SD 1.55) mumol/L of packed erythrocytes. Only negligible amounts of putrescine were found.
Gerbaut
L
L
Laboratoire de Pharmaco-Biochimie, Hôpital Saint Vincent de Paul, Paris, France.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003510:Cyclohexanes; D003619:Dansyl Compounds; D004912:Erythrocytes / Q000737:chemistry*; D006801:Humans; D011073:Polyamines / Q000097:blood*; D011700:Putrescine / Q000097:blood; D012016:Reference Values; D013095:Spermidine / Q000097:blood; D013096:Spermine / Q000097:blood
D003510:Cyclohexanes; D003619:Dansyl Compounds; D011073:Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine