Recognition by peptide mapping of three different structural groups of outer membrane protein YOP-1 of Yersinia enterocolitica and Yersinia pseudotuberculosis.
The structural relation of YOP-1 of "european" and "american" Yersinia enterocolitica serotypes O:3, O:9, O:5,27, and O:8 and O:20, respectively, and Y. pseudotuberculosis serotypes I, II, and III was compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis and peptide mapping using Staphylococcus aureus protease V8. Apparent molecular weights of YOP-1 ranged from 206,000 (O:3) to approx. 180,000 (O:8). According to their respective peptide maps YOP-1 of the "european" and "american" Y. enterocolitica serotypes and Y. pseudotuberculosis serotypes could be assigned to three different groups. Evaluation of several isolates of Y. enterocolitica serotypes O:3, O:9, and O:8 by peptide mapping indicated that YOP-1 is conserved within a serotype. However, one serotype O:8 isolate differed from the consensus peptide pattern of the other serotype O:8 and O:20 isolates. The similarity of the peptide patterns of Yersinia serotypes which predominate in certain geographical locations, i.e., "european" and "american" Y. enterocolitica serotypes, suggest common evolution of YOP-1 of these serotypes independent of the evolution of the other serotypes.
Mack
D
D
Institute for Medical Microbiology and Immunology, University Hospital Eppendorf, Hamburg, Federal Republic of Germany.
Pulz
M
M
Heesemann
J
J
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D010449:Peptide Mapping; D010957:Plasmids / Q000235:genetics; D012697:Serine Endopeptidases / Q000378:metabolism; D012703:Serotyping; D015008:Yersinia enterocolitica / Q000737:chemistry* / Q000145:classification / Q000276:immunology; D015011:Yersinia pseudotuberculosis / Q000737:chemistry* / Q000145:classification / Q000276:immunology
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; C064832:YadA protein, Yersinia; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase