[Directed introduction of amino groups by internucleotide phosphate group during solid-phase synthesis of oligodeoxyribonucleotides. Preparation of biotinylated oligonucleotide probes].
An efficient procedure for solid-phase synthesis of amino-oligonucleotides has been developed, leading to direct introduction of an aliphatic primary amino group at the internucleotide phosphate. Amino-oligonucleotides were successfully used for preparation of biotinylated oligonucleotides.
Veĭko
V P
VP
Ratmanova
K I
KI
Osipov
A S
AS
Bulenkov
M T
MT
Pugachev
V V
VV
D000588:Amines / Q000737:chemistry*; D001483:Base Sequence; D001710:Biotin / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D015345:Oligonucleotide Probes / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds / Q000737:chemistry*
D000588:Amines; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D009943:Organophosphorus Compounds; D001710:Biotin
Napravlennoe vvedenie aminogrupp po mezhnukleotidnoĭ fosfatnoĭ gruppe pri tverdofaznom sinteze oligodezoksiribonukleotidov. Poluchenie biotinilirovannykh oligonukleotidnykh zondov.