The effect of doxorubicin, daunorubicin and 4'-epidoxorubicin on the exogenous c-myc promoter in mouse erythroleukemia cells.
The recombinant plasmids p324, p330 and p323 carrying the aminoglycoside phosphotransferase (aph) gene and 5' flanking c-myc sequences linked to the reporter gene chloramphenicol acetyl-transferase (cat) were introduced into the mouse erythroleukemia cell line F412B2TK- and stable transfectants resistant to geneticin were obtained. The effects of three anthracycline compounds, doxorubicin, daunorubicin and 4'-epidoxorubicin, were studied using a large range of drug concentrations. It was found that 4'-epidoxorubicin and daunorubicin stimulate cat-gene expression with maximum effect at a concentration of 1 micrograms/ml, while doxorubicin enhances transcription from c-myc promoter at 5 micrograms/ml.
Eliopoulos
A
A
Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece.
Kerr
D J
DJ
Spandidos
D A
DA
D000595:Amino Acid Sequence; D000818:Animals; D003630:Daunorubicin / Q000494:pharmacology*; D004317:Doxorubicin / Q000494:pharmacology; D015251:Epirubicin / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D016259:Genes, myc / Q000187:drug effects*; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000187:drug effects
D015251:Epirubicin; D004317:Doxorubicin; D003630:Daunorubicin