Protein kinase C stimulation enhances the process formation of adult oligodendrocytes and induces proliferation.
Oligodendrocytes (OL) isolated from adult pig brains regenerate their processes and form a network of fibers after 14-18 days in vitro (DIV). Stimulation of protein kinase C (Pk-C) by tumour promoters such as 12-O-tetradecanoylphorbol-13-acetate (TPA) produces at day 7 in vitro a similar network after only 20 hr. H-7, an inhibitor of Pk-C, as well as amiloride, which inhibits the subsequent Na+/H+ exchange, reversibly suppress this effect. Activation of the protein kinase A or calmodulin pathway do not result in an increased OL process production. Furthermore, TPA induced proliferation in a subpopulation of OL. We conclude that the stimulation of Pk-C is of utmost importance for OL regeneration.
Althaus
H H
HH
Department of Neurochemistry, Max-Planck-Institut für experimentelle Medizin, Göttingen, Federal Republic of Germany.
Schröter
J
J
Spoerri
P
P
Schwartz
P
P
Klöppner
S
S
Rohmann
A
A
Neuhoff
V
V
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000166:cytology / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007546:Isoquinolines / Q000494:pharmacology*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009416:Nerve Regeneration / Q000187:drug effects*; D009836:Oligodendroglia / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D010879:Piperazines / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013552:Swine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D005904:Glial Fibrillary Acidic Protein; D007546:Isoquinolines; D010879:Piperazines; D000584:Amiloride; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate