Salbutamol identification in liver and urine by high-performance thin-layer chromatography and densitometry.
This paper describes a rapid method for the identification of salbutamol in liver and urine. Salbutamol is extracted from liver with an acid solution, purified on Baker columns and eluted with methanol. After derivatization, salbutamol is detected on HPTLC plates as a blue spot. Urine samples are directly purified on the C18 columns and then the same procedure is followed as for the liver samples. Using this screening method, salbutamol can be semi-quantitatively determined at the micrograms/kg level.
De Groof
J
J
Ministère de la Santé Publique et de l'Environnement, Bruxelles, Belgium.
Degroodt
J M
JM
Wyhowski de Bukanski
B
B
Beernaert
H
H
D000420:Albuterol / Q000032:analysis* / Q000302:isolation & purification / Q000652:urine; D002855:Chromatography, Thin Layer; D003720:Densitometry; D006801:Humans; D008099:Liver / Q000737:chemistry*; D015394:Molecular Structure
D000420:Albuterol