Measurement of plasma acetate kinetics using high-performance liquid chromatography.
Previous studies suggest that plasma acetate may be an important fuel in man, accounting for approximately 10% of energy expenditure. Available methods for the determination of plasma acetate kinetics are difficult and time consuming. We describe here a procedure for the determination of plasma acetate concentration and specific activity using automated high-performance liquid chromatography that is precise and sensitive and accommodates large numbers of samples. The procedure involves extraction from plasma with diethyl ether, derivatization with bromoacetophenone, and separation on a C-18 reversed-phase column. The specific activities of D-beta-hydroxybutyrate and lactate can also be determined. Acetate turnover was measured in four dogs and was similar to that previously reported in sheep and humans. Transport of [14C]acetate into red blood cells was negligible.
Persson
M
M
Endocrine Research Unit, Mayo Clinic, Rochester, Minnesota 55905.
Bleiberg
B
B
Kiss
D
D
Miles
J
J
D020155:3-Hydroxybutyric Acid; D000085:Acetates / Q000097:blood*; D000098:Acetophenones / Q000737:chemistry; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D004912:Erythrocytes / Q000378:metabolism; D006885:Hydroxybutyrates / Q000032:analysis; D007553:Isotope Labeling; D007700:Kinetics; D007773:Lactates / Q000032:analysis; D014316:Tritium
D000085:Acetates; D000098:Acetophenones; D006885:Hydroxybutyrates; D007773:Lactates; D014316:Tritium; C013190:phenacyl bromide; D020155:3-Hydroxybutyric Acid