Cell growth experiments using a microcalorimetric vessel equipped with oxygen and pH electrodes.
A method is described for simultaneous measurements of heat production rate, oxygen activity and pH, using a 3-ml stirred microcalorimetric titration/perfusion vessel fitted with a polarographic oxygen sensor and a combination pH-electrode. Heat production rate (+/- 0.3 microW), pH (+/- 0.01) and oxygen concentration (+/- 4 mumol/l) could be measured without any cross interference between the three signals. Results from measurements on suspensions of T-lymphoma cells and E. coli are presented.
Bäckman
P
P
Thermochemistry Division, University of Lund, Sweden.
Wadsö
I
I
D002151:Calorimetry / Q000295:instrumentation / Q000379:methods; D002455:Cell Division; D004566:Electrodes; D004926:Escherichia coli / Q000254:growth & development; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D016399:Lymphoma, T-Cell / Q000473:pathology; D010100:Oxygen / Q000378:metabolism*; D011048:Polarography / Q000295:instrumentation; D014407:Tumor Cells, Cultured
D008024:Ligands; D010100:Oxygen