A C-terminal proline is required for bioluminescence of the Ca(2+)-binding photoprotein, aequorin.
The requirement for a proline residue at the C-terminus of the Ca(2+)-binding photoprotein, aequorin, was investigated by measuring luminescence activities of a series of C-terminal deletion mutants, substitution mutants and an addition mutant. CD spectral measurements of apoaequorin with the C-terminal proline deleted showed a small change in secondary structure. In all cases studied, the C-terminal proline was required for bioluminescence activity.
Nomura
M
M
Osaka Bioscience Institute, Japan.
Inouye
S
S
Ohmiya
Y
Y
Tsuji
F I
FI
D000331:Aequorin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002942:Circular Dichroism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D049449:Luminescence; D008969:Molecular Sequence Data; D011392:Proline; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D015183:Restriction Mapping; D037401:Scyphozoa
D011994:Recombinant Proteins; D000331:Aequorin; D011392:Proline