[A method for selecting Streptomyces spheroides mutants--producers of an exoprotease with fibrinolytic activity].
The paper present a description of a fast method to obtain mutants of Streptomyces spheroides--supersynthetics of exoprotease. The primary culture spores being irradiated by UV-rays, the mutants are formed with presence in the cultural medium of own proteolytic enzyme added from without. The described method is based on the phenomenon of growth suppression in the primary culture spore by high concentrations of the own proteolytic enzyme. It permits selecting producers with high proteolytic activity of fibrinolytic proteases from the small number of mutants grown in these colonies.
Prianishnikova
N I
NI
Al'-Nuri
M A
MA
D001431:Bacteriological Techniques; D020689:Exopeptidases; D005343:Fibrinolytic Agents / Q000302:isolation & purification* / Q000528:radiation effects; D009154:Mutation; D010447:Peptide Hydrolases / Q000096:biosynthesis* / Q000302:isolation & purification / Q000528:radiation effects; D012641:Selection, Genetic; D013171:Spores, Bacterial / Q000201:enzymology / Q000528:radiation effects; D013302:Streptomyces / Q000201:enzymology* / Q000528:radiation effects; D014466:Ultraviolet Rays
D005343:Fibrinolytic Agents; D020689:Exopeptidases; D010447:Peptide Hydrolases
Metod otbora mutantov Streptomyces spheroides--produtsentov ékzoproteazy s fibrinoliticheskoĭ aktivnost'iu.