title: Hepatic vascular exclusion as a model for complete and stable hepatic ischemia in dogs.
abstract: In order to confirm a complete ischemia model, 1-hour warm hepatic ischemia by hepatic vascular exclusion (HVE) was studied in dogs, in comparison with that by inflow occlusion (IOC) only. The splanchnic venous bed and/or infrahepatic inferior vena cava were decompressed by a centripetal pump-driven venovenous bypass. Indocyanine green retention test revealed no hepatic blood flow in the HVE model during ischemia, while hepatic blood perfusion was still present in the IOC model. All 5 of the IOC dogs survived more than 7 days after revascularization, while 4 of the 5 HVE dogs died within 9 h. After the induction of hepatic ischemia, lactate increased in both HVE and IOC dogs. After revascularization, transaminases and guanase were elevated, the arterial ketone body ratio (acetoacetate/3-hydroxybutyrate) decreased and the serum lactate accumulated more in HVE dogs than in IOC dogs. The hepatic redox state of IOC dogs was significantly decreased by additional clamping of the inferior vena cava. It is concluded that the HVE model with a pump-driven active bypass provides complete and stable hepatic ischemia, resulting in greater deterioration of hepatic cellular functions; hence it is more suitable as a model of complete hepatic ischemia than the IOC one.
authors.affiliation: Second Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Egawa
authors.forename: T
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Yamaoka
authors.forename: Y
authors.initials: Y
authors.lastname: Taki
authors.forename: T
authors.initials: T
authors.lastname: Morimoto
authors.forename: N
authors.initials: N
authors.lastname: Yamamoto
authors.forename: J
authors.initials: J
authors.lastname: Ueda
authors.forename: Y
authors.initials: Y
authors.lastname: Konishi
authors.forename: R
authors.initials: R
authors.lastname: Kagawa
authors.forename: M
authors.initials: M
authors.lastname: Washida
mesh_terms: D000818:Animals; D001794:Blood Pressure; D004195:Disease Models, Animal; D004285:Dogs; D007208:Indocyanine Green; D007511:Ischemia / Q000209:etiology*; D007657:Ketone Bodies / Q000097:blood; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D008099:Liver / Q000098:blood supply*
chemical_list: D007657:Ketone Bodies; D007773:Lactates; D019344:Lactic Acid; D007208:Indocyanine Green