[RNA ligase from bacteriophage T4. VIII. Solid phase enzymatic synthesis of oligoribonucleotides].
A method of the solid-phase enzymic synthesis of oligoribonucleotides has been suggested. The donor is fixed through its 3'-end on a water-insoluble matrix followed by the stepwise RNA ligase- and T4 polynucleotide kinase-assisted coupling of trinucleoside diphosphates in the 5'-direction. As an example, (pA)6pAox was immobilised on Biogel P-300 hydrazine and the RNA ligase-catalyzed addition of acceptor ApApA to the donor gave (Ap)9 with the 50% yield.
Mudrakovskaia
A V
AV
Iamkovoĭ
V I
VI
D002853:Chromatography, Liquid; D006801:Humans; D007124:Immunoenzyme Techniques; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D011130:RNA Ligase (ATP) / Q000378:metabolism*; D013604:T-Phages / Q000201:enzymology*
D009843:Oligoribonucleotides; D011130:RNA Ligase (ATP)
RNK-ligaza bakteriofaga T4. VIII. Tverdofaznyĭ fermantativnyĭ sintez oligoribonukleotidov.