Echinococcus multilocularis antigens modify accessory cell function of macrophages.
Peritoneal macrophages and splenic lymphocytes were collected from BALB/c mice, normal or previously infected with Echinococcus multilocularis. In an accessory cell function assay, peritoneal macrophages, in increasing numbers, were added to cultures of splenic lymphocytes. Cultures were stimulated by concanavalin A (Con A) or E. multilocularis culture supernatant (EMSN). Post-infection macrophages, unlike normal macrophages, suppressed Con A- and EMSN-driven lymphocyte transformation. Modification of accessory cells could also be repeatedly induced in vivo by EMSN or a single FPLC fraction of EMSN. Lymphocytes were made more sensitive to accessory cell signals following incubation with EMSN.
Rakha
N K
NK
Department of Veterinary Pathology, Faculty of Veterinary Science, School of Tropical Medicine, University of Liverpool.
Dixon
J B
JB
Carter
S D
SD
Craig
P S
PS
Jenkins
P
P
Folkard
S
S
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology; D003208:Concanavalin A / Q000276:immunology; D004443:Echinococcosis / Q000276:immunology*; D004446:Echinococcus / Q000276:immunology; D007108:Immune Tolerance / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010529:Peritoneal Cavity / Q000166:cytology
D000947:Antigens, Helminth; D003208:Concanavalin A