title: Micromethod for PMN function studies in neonates.
abstract: Polymorphonuclear neutrophils play an important role in host defense mechanism. To assess PMN function in neonate a micromethod using only a few drops of blood was standardised. PMN adherent to coverslip were incubated with bacteria and using a differential staining with Acridine Orange (AO) phagocytic and bactericidal capacity were estimated.
authors.affiliation: Department of Pharmacology, Microbiology and Paediatrics, Seth G. S. Medical College, Parel, Bombay, Maharashtra.
authors.forename: J R
authors.initials: JR
authors.lastname: Samtani
authors.forename: S U
authors.initials: SU
authors.lastname: Wagle
authors.forename: S F
authors.initials: SF
authors.lastname: Irani
authors.forename: N A
authors.initials: NA
authors.lastname: Kshirsagar
mesh_terms: D001770:Blood Bactericidal Activity; D006801:Humans; D007231:Infant, Newborn / Q000097:blood* / Q000382:microbiology; D009504:Neutrophils / Q000502:physiology*; D010587:Phagocytosis / Q000502:physiology*