title: Gadolinium-DTPA-enhanced magnetic resonance imaging of the isolated rat heart after ischemia and reperfusion.
abstract: The objective of this study was to assess the potential of gadolinium-diethylenetriamine pentaacetic acid (Gd-DTPA) to identify myocardial ischemia and reperfusion in the isolated rat heart model. Ischemia was induced by reducing the perfusion pressure from 80 to 30 mm Hg for 2 hours. Hearts were not reperfused, or were reperfused for 20 minutes or for 2 hours. Perfusion was performed with Evans blue dye and/or Gd-DTPA for 3 minutes. Twenty isolated rat hearts were perfused according to the Langendorff method, and divided into five groups according to the perfusion status and the use of Gd-DTPA and/or Evans blue as perfusion markers. The Evans blue distribution in the hearts was assessed by point-counting volumetry. The Gd-DTPA distribution was assessed by magnetic resonance microimaging at 6.3 T field strength. Evans blue staining clearly identified areas with "no flow" or "no reflow." Perfusion with Gd-DTPA enhanced signal intensity significantly, both in ischemic and reperfused myocardium. Signal intensity in hearts reperfused for 2 hours was increased significantly compared to nonreperfused ischemic hearts, but not to ischemic hearts reperfused for 20 minutes. Magnetic resonance imaging with the aid of Gd-DTPA can identify ischemia and reperfusion in the isolated rat heart, dependent on residual perfusion.
authors.affiliation: Department of Diagnostic Radiology, University Hospital Leiden, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: de Roos
authors.forename: R W
authors.initials: RW
authors.lastname: Mohanlal
authors.forename: J J
authors.initials: JJ
authors.lastname: van Vaals
authors.forename: A H
authors.initials: AH
authors.lastname: Bergman
authors.forename: J
authors.initials: J
authors.lastname: Doornbos
authors.forename: N A
authors.initials: NA
authors.lastname: Matheijssen
authors.forename: E E
authors.initials: EE
authors.lastname: van der Wall
authors.forename: A
authors.initials: A
authors.lastname: van der Laarse
mesh_terms: D000818:Animals; D003287:Contrast Media; D003327:Coronary Disease / Q000175:diagnosis* / Q000473:pathology / Q000503:physiopathology; D005070:Evans Blue; D019786:Gadolinium DTPA; D066298:In Vitro Techniques; D008279:Magnetic Resonance Imaging; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000473:pathology; D009942:Organometallic Compounds; D004369:Pentetic Acid; D051381:Rats
chemical_list: D003287:Contrast Media; D009942:Organometallic Compounds; D005070:Evans Blue; D004369:Pentetic Acid; D019786:Gadolinium DTPA