title: A major Litomosoides carinii microfilarial sheath glycoprotein (gp22): amino terminal sequence and immunological studies with corresponding synthetic peptides.
abstract: The major glycoprotein of the sheath of Litomosoides carinii microfilariae (gp22) was analysed for its amino acid and amino sugar composition. It is rich in proline, glutamine/glutamic acid and glycine and contains (N-acetyl)galactosamine. The N-terminal amino acid sequence was determined up to position 37. It consists of a group of 6 repeats of the pentapeptide sequence methionine-glycine-proline-glutamine-proline with two minor modifications in repeats 3-6, while the first two repeats follow the general pattern more loosely. Identical N-terminal amino acid sequences were found in at least two other sheath polypeptides (33 kDa, 39 kDa). Antisera prepared against 3 overlapping synthetic peptides corresponding to the amino terminus of gp22 recognized different epitopes. They all reacted with identical patterns of sheath polypeptides. The antisera failed to recognize antigens of 4th-stage larvae of L. carinii. In contrast, cross-reacting epitopes were detected in other parasite stages. Antisera reacted with material surrounding embryos and microfilariae in the uterus of females, and caused patchy fluorescence on the sheath of blood-derived and in vitro-released microfilariae.
authors.affiliation: Biochemisches Institut am Klinikum, Justus-Liebig-Universität, Giessen, FRG.
authors.forename: G
authors.initials: G
authors.lastname: Bardehle
authors.forename: F J
authors.initials: FJ
authors.lastname: Conraths
authors.forename: F
authors.initials: F
authors.lastname: Fahrenholz
authors.forename: M
authors.initials: M
authors.lastname: Hintz
authors.forename: D
authors.initials: D
authors.lastname: Linder
authors.forename: G
authors.initials: G
authors.lastname: Schares
authors.forename: H H
authors.initials: HH
authors.lastname: Schott
authors.forename: B
authors.initials: B
authors.lastname: Schützle
authors.forename: S
authors.initials: S
authors.lastname: Stirm
authors.forename: W
authors.initials: W
authors.lastname: Stüber
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000606:Amino Sugars / Q000032:analysis; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005370:Filarioidea / Q000737:chemistry* / Q000276:immunology; D006023:Glycoproteins / Q000737:chemistry* / Q000276:immunology; D015801:Helminth Proteins / Q000737:chemistry* / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D008842:Microfilariae / Q000737:chemistry / Q000276:immunology; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology
chemical_list: D000596:Amino Acids; D000606:Amino Sugars; D006023:Glycoproteins; D015801:Helminth Proteins; D007106:Immune Sera; D010455:Peptides