Microcinematographic analysis of further fate of multinucleate cells arising after polyethylene glycol induced fusions of L cells.
Cell fusions were studied in continuous cell line L treated with polyethylene glycol (PEG). The progenies of 152 treated cells were studied by time-lapse microcinematography up to four generations. It was found that all the cells that remained mononucleate after treatment with PEG grew actively and their mitotic divisions were without abnormalities. On the contrary, only 60% of multinucleated (fused) cells divided mitotically during the filming period. We observed many disturbances in mitotic divisions of multinucleated cells. In most cases the multipolar divisions of these cells resulted in mononucleate daughters. In half of these cases the daughter cells fused to form again multinucleate cells. Nevertheless, both the total number of multinucleate cells and the average number of nuclei per cell were lowered after mitoses.
Cervinka
M
M
Katedra biologie LF UK, Hradec Králové.
D000818:Animals; D002459:Cell Fusion / Q000187:drug effects*; D002467:Cell Nucleus / Q000648:ultrastructure; D007739:L Cells / Q000166:cytology / Q000648:ultrastructure; D051379:Mice; D008938:Mitosis; D009040:Motion Pictures; D011092:Polyethylene Glycols / Q000494:pharmacology*
D011092:Polyethylene Glycols