[The radioprotective effect of a hypoxic respiratory mixture (8% O2) for intestinal epithelial stem cells during single and fractionated irradiation of mice].
The method of intestinal "microcolonies" was used to study the radioprotective effect of a gas mixture, containing 8% of O2, on mice subjected to single and fractionated (5 fractions for 30 min) irradiation. The protective effect was indicated by a decreased slope of dose curves of the stem cell injury; the extrapolation number decreased simultaneously. So the values of dose modifying factors (DMF) were higher, when calculated by D0 ratio (where they amounted to 1.76 and 1.39 for single and fractionated exposure respectively), than those determined by equally effective doses (1.19 and 1.26 for single and fractionated effects respectively, which corresponded to LD50/4 when calculated at lg N = 1.9). It is suggested that the radiation response of certain stem cell populations of intestinal epithelium are different: this is attributed to different degrees of hypoxia in cells and to different directions of the hypoxia effects on the injury and the ability of postirradiation repair.
Konopliannikova
O A
OA
Konopliannikov
A G
AG
Zaĭtsev
A V
AV
Waĭnson
A A
AA
D000818:Animals; D004847:Epithelial Cells; D004848:Epithelium / Q000528:radiation effects; D007422:Intestines / Q000166:cytology / Q000528:radiation effects; D008297:Male; D051379:Mice; D010100:Oxygen / Q000502:physiology*; D011829:Radiation Dosage; D011833:Radiation Injuries, Experimental / Q000503:physiopathology / Q000517:prevention & control*; D013234:Stem Cells / Q000528:radiation effects*
D010100:Oxygen
Radiozashchitnoe deĭstvie gipoksicheskoĭ dykhatel'noĭ smesi (8% O2) dlia stvolovykh kletok kishechnogo épiteliia pri odnokratnom i fraktsionirovannom obluchenii mysheĭ.