Enzyme-linked immunosorbent assays for bovine alpha-lactalbumin and beta-lactoglobulin in serum and tissue culture media.
Enzyme-linked immunosorbent assays for bovine alpha-lactalbumin and beta-lactoglobulin have been developed for measurements of serum and tissue culture samples. Either alpha-lactalbumin or beta-lactoglobulin antiserum was coated on ELISA plates. Biotinylated proteins were used in competition with unknown amount of proteins in samples. After unbound proteins were washed off, ExtrAvidin-peroxidase and tetramethylbenzidine were then used as a detection system. Crossreactivity of caseins or bovine serum albumin was less than .0001% in either alpha-lactalbumin or beta-lactoglobulin ELISA. Parallel curves from serial dilutions were obtained in serum and media samples. The additivity of alpha-lactalbumin and beta-lactoglobulin ELISA was validated in either serum or medium samples. The intraassay and interassay coefficients of variation for alpha-lactalbumin and beta-lactoglobulin ELISA were below 10% over 51 and 47 assays. The ELISA are useful in mammary gland biology studies for measuring milk whey protein in serum or culture media.
Mao
F C
FC
Endocrinology-Reproductive Physiology Program, University of Wisconsin, Madison 53706.
Bremel
R D
RD
D000818:Animals; D001667:Binding, Competitive; D002417:Cattle / Q000502:physiology*; D003429:Cross Reactions; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007106:Immune Sera / Q000276:immunology; D007768:Lactalbumin / Q000032:analysis* / Q000097:blood / Q000276:immunology; D007782:Lactoglobulins / Q000032:analysis* / Q000097:blood / Q000276:immunology; D008894:Milk Proteins / Q000276:immunology
D003470:Culture Media; D007106:Immune Sera; D007782:Lactoglobulins; D008894:Milk Proteins; D007768:Lactalbumin