[Synthesis of delta virus cDNA of China Sichuan strain].
delta virus cDNA of China Sichuan strain, 1.2 kb in length, was synthesized by extraction of RNA from liver tissue in a patient with subacute severe hepatitis, followed by reverse transcription and polymerase chain reaction (RT-PCR). The result was confirmed by Southern blot hybridization using 32P and biotin labelled delta virus cDNA (HN88, cloned in USA) probes respectively.
Zhao
L
L
Research Unit of Viral Hepatitis, Institute of Biochemistry and Molecular Biology.
Zhou
S
S
Lin
Y
Y
Wang
J
J
Liu
B
B
D015139:Blotting, Southern; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D003698:Hepatitis Delta Virus / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000382:microbiology; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic
D004279:DNA, Viral