Detection of equine antibody to Babesia equi merozoite proteins by a monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay.
A competitive inhibition enzyme-linked immunosorbent assay (CI ELISA) was developed to detect antibody to Babesia equi. One hundred fifty-four equine serum samples from 19 countries were tested for antibody to B. equi by the complement fixation test and by CI ELISA. The CI ELISA and complement fixation test results agreed in 94% (144) of the serum samples tested. The 10 discrepant serum samples were retested and analyzed for ability to immunoprecipitate in vitro translation products from B. equi merozoite mRNA. Five discrepant results were clearly resolved in favor of the CI ELISA, and the remaining five discrepancies were not definitively resolved.
Knowles
D P
DP
Animal Disease Research Unit, USDA-ARS, Pullman, Washington 99164-7030.
Perryman
L E
LE
Kappmeyer
L S
LS
Hennager
S G
SG
D000818:Animals; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan / Q000032:analysis*; D001403:Babesia / Q000276:immunology*; D001404:Babesiosis / Q000175:diagnosis / Q000276:immunology; D001667:Binding, Competitive; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D006734:Horse Diseases / Q000175:diagnosis / Q000276:immunology; D006736:Horses; D015800:Protozoan Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D015800:Protozoan Proteins