Time-dependent AluI action on human chromosomes.
We have analyzed the pattern of AluI digestion over time on human chromosomes in order to monitor the evolution of the in situ enzyme action. Short treatments followed by Giemsa staining produce a G-like banding effect, whereas longer treatments produce a C-like banding pattern. However, when Propidium iodide staining is used, it reveals a uniform bright fluorescence after short AluI digestions and C bands when longer treatments are developed. We propose that C banding is the result of a uniform DNA removal in non centromeric regions taking place after a critical time point, the initial G like banding being produced by changes in the DNA-proteins interactions.
Fernández-Peralta
A M
AM
Departamento de Biología, Facultad de Ciencias C.XV, Universidad Autónoma de Madrid, Spain.
Tagarro
I
I
Ludeña Reyes
P
P
Sentís Castaño
C
C
Fernández Piqueras
J
J
González-Aguilera
J J
JJ
D002478:Cells, Cultured; D002871:Chromosome Banding; D002875:Chromosomes / Q000187:drug effects*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000494:pharmacology*; D006801:Humans
C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific