[Analysis of neonatal and fetal blood for the diagnosis of congenital toxoplasmosis infection].
A total of 175 serum samples, including 35 obtained from neonates and 5 from fetuses in whom connatal toxoplasma infection was suspected, were simultaneously tested for toxoplasma-specific IgA and IgM antibodies by immunosorbent agglutination assay (ISAGA). If sufficient serum was available, IgM-IIFT (Remington test) was also performed prior to and following IgG absorption. All the control samples from children not infected with toxoplasma (SFT negative, or titers less than or equal to 1:256) were negative in the IgA and IgM ISAGA. Of the 35 congenitally infected children, results were positive in 91.4% in the IgA and/or the IgM ISAGA. Of 5 fetuses with positive results in animal experiments 3 were positive in the IgM ISAGA. The serum from the fourth fetus was low positive in the IgM IIFT, while that from the fifth fetus was both IgM- and IgA-negative. The results of this study show that several methods should always be used simultaneously to detect connatal toxoplasma infection.
Saathoff
M
M
Institut für Medizinische Parasitologie, Universität Bonn.
Seitz
H M
HM
D000913:Antibodies, Protozoan / Q000302:isolation & purification*; D005312:Fetal Blood / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007070:Immunoglobulin A / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D007163:Immunosorbent Techniques; D007231:Infant, Newborn; D014125:Toxoplasmosis, Congenital / Q000276:immunology*
D000913:Antibodies, Protozoan; D007070:Immunoglobulin A; D007075:Immunoglobulin M
Untersuchung von Neugeborenen- und Fetalseren zur Erfassung von konnatalen Toxoplasma-Infektionen.