Fast atom bombardment tandem mass spectrometric analysis of N-carbamoylamino acids.
Using fast atom bombardment (FAB) and tandem mass spectrometry (MS/MS), we examined 12 synthetic N-carbamoylamino acids (CAA) as tert-butyldimethylsilyl (TBDMS) derivatives. In FAB mass spectrometry and FAB MS/MS, spectra of protonated molecules for CAA provide specific cleavages involving the TBDMS carbamoyl moiety. The daughter scan spectrum of the parent ion indicated that it was useful for structural elucidation and differentiation of structural isomers of CAA. We have also identified each CAA separately in a mixture using a neutral loss scan for characteristic ions. In addition, we demonstrated that CAA in urine samples from patients with ornithine carbamoyl transferase deficiency gave collision-induced dissociation (CID) spectra which correspond well with CID spectra obtained using synthetically prepared CAA.
Inoue
Y
Y
Division of Human Genetics, Kanazawa Medical University, Ishikawa, Japan.
Ohkura
T
T
Matsumoto
I
I
Rudewicz
P J
PJ
D000596:Amino Acids / Q000032:analysis*; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000652:urine; D002219:Carbamates / Q000032:analysis*; D006801:Humans; D020163:Ornithine Carbamoyltransferase Deficiency Disease; D016339:Spectrometry, Mass, Fast Atom Bombardment; D015091:beta-Alanine / Q000031:analogs & derivatives / Q000652:urine
D000596:Amino Acids; D002219:Carbamates; D015091:beta-Alanine; D001224:Aspartic Acid; C035122:N-carbamoyl-beta-alanine; C006893:ureidosuccinic acid