Yeast artificial chromosome-based genome mapping: some lessons from Xq24-q28.
Yeast artificial chromosomes (YACs) have recently provided a potential route to long-range coverage of complex genomes in contiguous cloned DNA. In a pilot project for 50 Mb (1.5% of the human genome), a variety of techniques have been applied to assemble Xq24-q28 YAC contigs up to 8 Mb in length and assess their quality. The results indicate the relative strength of several approaches and support the adequacy of YAC-based methods for mapping the human genome.
Schlessinger
D
D
Department of Molecular Microbiology, Washington University School Medicine, St. Louis, Missouri 63110.
Little
R D
RD
Freije
D
D
Abidi
F
F
Zucchi
I
I
Porta
G
G
Pilia
G
G
Nagaraja
R
R
Johnson
S K
SK
Yoon
J Y
JY
Srivastava
A
A
Kere
J
J
Palmieri
G
G
Ciccodicola
A
A
Montanaro
V
V
Romano
G
G
Casamassimi
A
A
D'Urso
M
M
D002874:Chromosome Mapping / Q000379:methods*; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D015894:Genome, Human; D006801:Humans; D014960:X Chromosome