title: Steroid hormone specifically binds to rat kidney plasma membrane.
abstract: A high-affinity and low-capacity corticosterone specific binding was detected in the purified plasma membrane preparation from rat kidney using an in vitro steroid hormone binding assay. The specific-bound hormone was efficiently distinguished from the irreversible-bound hormone with 10 microM corticosterone. Under standardized conditions of pH 7.4 at 2 degrees C and 30 min incubation time, the binding was saturable and showed Kd = 13 +/- 3 nM and Bmax = 616 +/- 34 fmol/mg of protein. Competitive binding studies with analogue steroids indicated that corticosterone binding to kidney plasma membrane is hormone-specific. Results indicated that the possible nongenomic effects of steroids could be mediated by their interaction with plasma membrane.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad del País Vasco/Euskal Herriko Unibersitatea, Bilbao, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Ibarrola
authors.forename: K
authors.initials: K
authors.lastname: Ogiza
authors.forename: A
authors.initials: A
authors.lastname: Marino
authors.forename: J M
authors.initials: JM
authors.lastname: Macarulla
authors.forename: M
authors.initials: M
authors.lastname: Trueba
mesh_terms: D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D003345:Corticosterone / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D013256:Steroids / Q000378:metabolism
chemical_list: D013256:Steroids; D003345:Corticosterone