title: [Molecular forms of alpha-1-acid glycoprotein in human blood serum. Differences in levels of di-, tri-, and tetra-antennary N-linked carbohydrate chains].
abstract: alpha 1-Acid glycoprotein isolated from healthy individuals blood was separated on Con A-Sepharose into three fractions: non-bound (AGP-1, 84%, 43.5 kDa), Con A-bound (AGP-2, 14%, 41.3 kDa), and Con A-tightly bound (AGP-3, 2%, 39.6 kDa). Amino acid compositions of these fractions were similar but carbohydrate ones differed. HPLC analysis of 7-amino-4-methylcoumarin derivatives of the oligosaccharides in combination with their sequential exoglycosidase digestion showed that AGP-1, AGP-2, and AGP-3 have the same set of oligosaccharides and differ only by their proposition. A minor quantity of agalacto-oligosaccharides (with a terminal GlcNAc residue) was identified.
authors.forename: S D
authors.initials: SD
authors.lastname: Shiian
authors.forename: V V
authors.initials: VV
authors.lastname: Nasonov
authors.forename: N V
authors.initials: NV
authors.lastname: Bovin
authors.forename: L I
authors.initials: LI
authors.lastname: Novikova
authors.forename: V A
authors.initials: VA
authors.lastname: Aleshkin
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000737:chemistry*; D005456:Fluorescent Dyes; D005690:Galactose / Q000737:chemistry; D006801:Humans; D009005:Monosaccharides / Q000032:analysis; D009961:Orosomucoid / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D005456:Fluorescent Dyes; D009005:Monosaccharides; D009961:Orosomucoid; D005690:Galactose
vernacular_title: Molekuliarnye formy alpha-1-kislogo glikoproteina syvorotki krovi cheloveka. Razlichiia v soderzhanii di-, tri-, i tetraantennykh N-uglevodnykh tsepeĭ.