First expression of protamine message in trout testis.
In situ hybridizations were performed using a biotinylated riboprobe complementary to protamine messenger RNA in order to directly examine the various cell types in the trout testis for the presence of protamine message. Computer-aided optical density measurements were used to provide estimates of transcript abundance for cells identified by their DAPI-labeled nuclei. Optically detectable protamine hybridization occurred only in spermatid cells. These findings are in accord with results obtained in other species which report protamine mRNA only in the post-meiotic spermatid cell; but they are in conflict with a previous study employing solution hybridization which noted that protamine message first appears in the spermatocytes of rainbow trout.
Seniuk
N A
NA
Centre for Research in Neurodegenerative Diseases, University of Toronto, Ontario, Canada.
Tatton
W G
WG
Cannon
P D
PD
Garber
A T
AT
Dixon
G H
GH
D000818:Animals; D003720:Densitometry; D005455:Fluorescent Antibody Technique; D008297:Male; D011479:Protamines / Q000378:metabolism*; D015347:RNA Probes; D012333:RNA, Messenger / Q000378:metabolism; D012474:Salmon; D013087:Spermatids / Q000378:metabolism; D013090:Spermatocytes / Q000378:metabolism; D013093:Spermatogonia / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism*
D011479:Protamines; D015347:RNA Probes; D012333:RNA, Messenger