Purine and pyrimidine compounds in murine peritoneal macrophages cultured in vitro.
Extracts of murine peritoneal macrophages were analysed by ion-pair reversed-phase high-performance liquid chromatography during incubation at 37 degrees C in vitro. Four-step gradient elution was applied to an ODS column (250 x 4.6 mm I.D.) at a flow-rate of 1.3 ml/min, allowing the separation of hypoxanthine, inosine, guanosine, adenosine, IMP, CDP, AMP, GDP, UDP, ADP, CTP, GTP, UTP and ATP within 50 min. Samples of 0.4 . 10(6)-0.5 . 10(6) cells were washed twice with RPMI 1640 medium and extracted with perchloric acid. Nucleotide concentrations of murine peritoneal macrophages did not change during incubation for 4 days in vitro.
Werner
A
A
Institute of Biochemistry, Berlin, Germany.
Maeba
R
R
Shimasaki
H
H
Ueta
N
N
Gerber
G
G
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008214:Lymphocytes / Q000737:chemistry; D008223:Lymphoma / Q000737:chemistry; D008264:Macrophages / Q000737:chemistry*; D051379:Mice; D009504:Neutrophils / Q000737:chemistry; D010529:Peritoneal Cavity / Q000166:cytology; D011687:Purines / Q000032:analysis*; D011743:Pyrimidines / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D011687:Purines; D011743:Pyrimidines