Isolation of the 66-kilodalton polypeptide from promastigotes of Leishmania donovani as a ligand molecule for binding to macrophages.
A 66-kDa major plasma membrane-associated molecule of promastigotes of Leishmania donovani (UR6) was purified by affinity chromatography. The immunoreactivity of the 66-kDa molecule was lost upon exposure to heat or treatment with trypsin. The metaperiodate oxidation significantly reduced its immunoreactivity. The 66-kDa molecule is, therefore, glycoprotein in nature. With a fluorescent probe, the 66-kDa molecule was found to be located on the tip of flagellum and on the kinetoplast. The exposure of promastigotes of L. donovani to monospecific anti-66-kDa antibodies significantly reduced the percentage of macrophages with attached promastigotes in the cultured cell line (J774G8). The data suggested that promastigotes of L. donovani utilize the 66-kDa molecule in recognizing and as ligand for binding to macrophages.
Vinayak
V K
VK
Department of Experimental Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Singla
N
N
Dutt
P
P
Mahajan
D
D
D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000032:analysis; D015153:Blotting, Western; D002462:Cell Membrane / Q000276:immunology; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D007120:Immunochemistry; D007893:Leishmania donovani / Q000737:chemistry*; D008024:Ligands; D008264:Macrophages / Q000378:metabolism*; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification*; D011485:Protein Binding; D015800:Protozoan Proteins / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D008024:Ligands; D010455:Peptides; D015800:Protozoan Proteins