Plastid DNA synthesis and nucleic acid-binding proteins in developing barley chloroplasts.
Activation of plastid DNA synthesis occurred early in barley leaf chloroplast development. High rates of DNA synthesis were observed in the leaf basal meristem of dark-grown plants where plastid transcription activity was low. DNA synthesis activity decreased in later stages of chloroplast development. Plastid nuclei acid-binding proteins were detected after lithium dodecylsulfate (LDS)-polyacrylamide gel separation and renaturation. One set of nucleic acid-binding proteins was associated with plastid nucleoids. A second set of nucleic acid-binding proteins co-sedimented with ribosomes and most probably corresponds to ribosomal proteins. Changes in the composition of the nucleic acid-binding proteins were characterized as a function of chloroplast development in dark-grown and illuminated barley plants.
Baumgartner
B J
BJ
Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843-2128.
Mullet
J E
JE
D002736:Chloroplasts / Q000187:drug effects / Q000502:physiology*; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D003624:Darkness; D004591:Electrophoresis, Polyacrylamide Gel; D001467:Hordeum / Q000502:physiology*; D008027:Light; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D004247:DNA