Access to gastric tissue promotes the survival of axotomized neurons in the dorsal motor nucleus of the vagus in neonatal rats.
Lesioning the vagus nerve in the neck (cervical vagotomy) results in a rapid and virtually complete loss of motoneurons in the dorsal motor nucleus of the vagus in neonatal rats. The present study sought to determine whether access to gastric target tissue will promote the survival of these motoneurons after axotomy. Quantitative analysis demonstrates that subdiaphragmatic vagotomy, which leaves the cut vagal axons in close proximity to their normal gastric targets, results in significantly less motoneuron loss than cervical vagotomy. Furthermore, the loss of motoneurons after cervical vagotomy can be significantly reduced by transplanting embryonic gastric tissue to the neck of vagotomized neonatal host rats, in the vicinity of the cut axons. The survival effect of transplanted gastric tissue appears specific because control transplants of embryonic bladder tissue fail to reduce motoneuron death after cervical vagotomy. Injections of the neural tracers Fluoro-Gold and cholera toxin-horseradish peroxidase into gastric transplants labeled surviving motoneurons in cervically vagotomized rats, whereas tracer injections into bladder transplants or into host cervical tissues did not. These results indicate that neonatal vagal motoneurons are capable of making the adjustments necessary to survive axotomy if they have access to gastric target cells. The apparent dependence of injured neonatal vagal motoneurons on gastric tissue offers a new system in which to examine in vivo the trophic interactions between neurons and their targets.
Rinaman
L
L
Department of Anatomy and Neurobiology, Medical College of Pennsylvania, Philadelphia 19129.
Levitt
P
P
D000818:Animals; D000831:Animals, Newborn; D002450:Cell Communication; D002470:Cell Survival; D016332:Fetal Tissue Transplantation; D009046:Motor Neurons / Q000502:physiology*; D009333:Neck; D009475:Neurons, Afferent / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000196:embryology / Q000502:physiology* / Q000637:transplantation; D016042:Transplantation, Heterotopic; D001743:Urinary Bladder / Q000637:transplantation; D014628:Vagotomy; D014630:Vagus Nerve / Q000502:physiology*; D061223:Vagus Nerve Injuries