title: Chemiluminescence dot blot hybridization assay for detection of B19 parvovirus DNA in human sera.
abstract: A chemiluminescence dot blot hybridization assay was used for the detection of B19 parvovirus DNA in human sera by using digoxigenin-labeled probes. The probes were revealed immunoenzymatically by use of anti-digoxigenin Fab fragments conjugated with alkaline phosphatase. The chemiluminescence signal was obtained by reacting the labeled probe-target complex with an enzyme-triggerable dioxetane substrate. The emitted photons were detected with instant photographic films. In the search for B19 parvovirus DNA, 2,808 serum samples were analyzed.
authors.affiliation: Istituto di Microbiologia, Universitá di Bologna, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Musiani
authors.forename: M
authors.initials: M
authors.lastname: Zerbini
authors.forename: D
authors.initials: D
authors.lastname: Gibellini
authors.forename: G
authors.initials: G
authors.lastname: Gentilomi
authors.forename: S
authors.initials: S
authors.lastname: Venturoli
authors.forename: G
authors.initials: G
authors.lastname: Gallinella
authors.forename: E
authors.initials: E
authors.lastname: Ferri
authors.forename: S
authors.initials: S
authors.lastname: Girotti
mesh_terms: D015342:DNA Probes; D004279:DNA, Viral / Q000097:blood* / Q000235:genetics; D004076:Digoxigenin; D016731:Erythema Infectiosum / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D015151:Immunoblotting / Q000379:methods* / Q000706:statistics & numerical data; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization; D016732:Parvovirus B19, Human / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D004076:Digoxigenin