title: c-Fos response to administration of catecholamines into brain by microdialysis.
abstract: The present studies investigated the use of microdialysis for the infusion of catecholamines into the brain in studies of immediate early gene (IEG) activation. c-Fos like immunoreactivity was examined histochemically in cortical tissue surrounding a dialysis probe implanted in the medial prefrontal cortex. No c-fos reactivity was observed at 48 h post implantation in control animals not probe-infused or infused 2 h earlier with Ringer's buffer. Marked reactivity was found in animals infused with a solution of norepinephrine (NE). The latter was blocked by coinfusion of the beta adrenoceptor antagonist, timolol. It is concluded that direct infusion of NE by dialysis can induce c-fos expression in cells of the cerebral cortex and that this effect is primarily via beta-adrenoceptors. Microdialysis therefore represents a useful technique in studies of IEG responses to brain catecholamines.
authors.affiliation: Department of Psychiatry, New York University School of Medicine, NY 10016.
authors.forename: E A
authors.initials: EA
authors.lastname: Stone
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: S M
authors.initials: SM
authors.lastname: John
authors.forename: G
authors.initials: G
authors.lastname: Bing
mesh_terms: D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D002395:Catecholamines / Q000008:administration & dosage / Q000494:pharmacology*; D003956:Dialysis; D005625:Frontal Lobe / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016762:Genes, fos / Q000187:drug effects*; D008297:Male; D009638:Norepinephrine / Q000008:administration & dosage / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013238:Stereotaxic Techniques; D013999:Timolol / Q000494:pharmacology
chemical_list: D002395:Catecholamines; D012333:RNA, Messenger; D013999:Timolol; D009638:Norepinephrine