Platelets process adsorbed protein: a morphological study.
The nature of the adsorbed protein on a non-biological biomaterial surface is known to influence cell adhesion. This work deals with the contribution of adherent platelets towards the redistribution of preadsorbed proteins. A redistribution was only observed with spread platelets on adsorbed fibrinogen and fibronectin, but not with adsorbed beta-lactoglobulin. The labelled protein images showed a dark outer ring with an inner region having fluorescent areas, with greater intensity than regions not covered by platelets. The fluorescent patterns observed may be a result of the binding of adsorbed fluorescent protein to the glycoprotein IIb/IIIa receptors of platelets, followed by their redistribution within the plasma membrane.
Gaebel
K
K
Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
Feuerstein
I A
IA
D000327:Adsorption; D000328:Adult; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000502:physiology / Q000648:ultrastructure*; D001798:Blood Proteins / Q000493:pharmacokinetics*; D005340:Fibrinogen / Q000493:pharmacokinetics; D005353:Fibronectins / Q000493:pharmacokinetics; D005453:Fluorescence; D006801:Humans; D007782:Lactoglobulins / Q000493:pharmacokinetics; D008768:Methylmethacrylates; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D010973:Platelet Adhesiveness / Q000502:physiology*
D001672:Biocompatible Materials; D001798:Blood Proteins; D005353:Fibronectins; D007782:Lactoglobulins; D008768:Methylmethacrylates; D005340:Fibrinogen