The simplified 13C-urea breath test--one point analysis for detection of Helicobacter pylori infection.
A 13C-urea breath test for detection of Helicobacter pylori infection was validated in 306 patients. Breath samples (four, two or one) were taken at various time points within 30 minutes after a motility inhibiting liquid test meal with citric acid followed by 75 mg of 13C-urea. The 13CO2/12 CO2-ratio (delta-value) was measured using isotope ratio mass spectrometry and the recovery of tracer in the exhaled breath was calculated (UBT-value). In 172 patients a comparison of established reference methods (culture, CLO test, and Fuchsin staining) with the 4 point breath analysis for detection of Helicobacter pylori showed high values of quality parameters (greater than 80% for sensitivity, specificity, positive and negative predictive value), independent of expression of the results as delta-value or as UBT-value. In 134 patients a reduction of samples to one single breath sample taken 30 minutes after ingestion of the tracer showed no significant differences in the quality parameters when compared with the standard 4 point breath analysis. This indicates that the analysis of a single breath sample is suitable for detection of Helicobacter pylori status in man.
Lotterer
E
E
Department of Clinical Pharmacology, University of Goettingen, Bundesrepublik Deutschland.
Ramaker
J
J
Lüdtke
F E
FE
Tegeler
R
R
Geletneky
J V
JV
Bauer
F E
FE
D001944:Breath Tests / Q000379:methods*; D002250:Carbon Radioisotopes; D003937:Diagnosis, Differential; D004381:Duodenal Ulcer / Q000175:diagnosis* / Q000382:microbiology; D016099:Endoscopy, Gastrointestinal; D005753:Gastric Mucosa / Q000382:microbiology; D005756:Gastritis / Q000175:diagnosis* / Q000382:microbiology; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology; D016480:Helicobacter pylori / Q000302:isolation & purification; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology; D013276:Stomach Ulcer / Q000175:diagnosis* / Q000382:microbiology; D014508:Urea
D002250:Carbon Radioisotopes; D014508:Urea