Protein adsorption on biomedical polymers with a phosphorylcholine moiety adsorbed with phospholipid.
The effects of phospholipid adsorption onto the polymer surface during adsorption of plasma proteins were investigated. When a polymer with the phosphorylcholine moiety, 2-methacryloyloxyethyl phosphorylcholine (MPC) co-polymer, was treated with dipalmitoylphosphatidylcholine (DPPC) liposome solution, an organized adsorption layer of DPPC was formed on the MPC co-polymer surface, which was confirmed by differential scanning calorimetric analysis and X-ray photoelectron spectroscopy. On the other hand, an organized layer of DPPC on poly(n-butyl methacrylate) and poly(2-hydroxyethyl methacrylate) could not be found. The amount of albumin adsorbed on the polymer surfaces was decreased by pretreatment of the surface with DPPC liposome solution in every polymer case. The smallest amount of adsorbed proteins was found on the MPC co-polymer. Protein adsorption on the surface of MPC co-polymers from the plasma was also small. The difference in protein adsorption on the polymers probably reflects the difference in the orientation of the phospholipid molecules which cover the polymer surface.
Ueda
T
T
Institute for Medical and Dental Engineering, Tokyo Medical and Dental University, Japan.
Watanabe
A
A
Ishihara
K
K
Nakabayashi
N
N
D000327:Adsorption; D000818:Animals; D001704:Biopolymers; D008567:Membranes, Artificial; D008689:Methacrylates; D010713:Phosphatidylcholines; D010743:Phospholipids; D010767:Phosphorylcholine / Q000031:analogs & derivatives; D011506:Proteins / Q000493:pharmacokinetics*; D011817:Rabbits; D013499:Surface Properties / Q000187:drug effects
D001704:Biopolymers; D008567:Membranes, Artificial; D008689:Methacrylates; D010713:Phosphatidylcholines; D010743:Phospholipids; D011506:Proteins; D010767:Phosphorylcholine; C028810:dodecylphosphocholine; C070638:2-methacryloyloxyethyl phosphorylcholine