[Chromatographic analysis and immunobiologic properties of tuberculins].
Immunobiologic activities of tuberculin preparations and their components have been comparatively studied using gel filtration and high-performance liquid chromatography (HPLC) techniques. It is shown that high-molecular weight fraction of protein-purified derivate tuberculin (PPD) had higher activity as compared to nonfractionated preparation in skin tests on guinea pigs sensitized with Mycobacterium bovis BCG as well as in enzyme-linked immunosorbent assay with affinity purified rabbit antibodies against PPD. Using the preparative HPLC-technique we failed to isolate a component of PPD having greater tuberculin test potency than nonfractionated preparation.
Komissarenko
S V
SV
Bobrovnik
S A
SA
Liashchenko
K P
KP
Nartikov
A V
AV
Petrova
Iu I
IuI
Gorbenko
E V
EV
D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D008970:Molecular Weight; D009163:Mycobacterium bovis / Q000276:immunology; D014373:Tuberculin / Q000032:analysis* / Q000276:immunology; D014374:Tuberculin Test
D014373:Tuberculin
Khromatograficheskiĭ analiz i immunobiologicheskie svoĭstva tuberkulinov.