Near on-line detection of enteric bacteria using lux recombinant bacteriophage.
The potential for a revolution in microbial testing can be perceived with the near on-line detection of indicator microorganisms. By definition, these are microorganisms present in significant numbers within a food which, while not pathogenic, can be related through increasing count to the increased probability of pathogen contamination. We have used recombinant lux+ bacteriophage to detect enteric indicator bacteria without recovery or enrichment in 50 min, provided that they are present at levels greater than 10(4) g-1 or cm-2. After a 4-h enrichment, samples having enteric counts of 10 g-1 or cm-2 can be distinguished from background.
Kodikara
C P
CP
University of Nottingham, Department of Applied Biochemistry and Food Science, Sutton Bonington, U.K.
Crew
H H
HH
Stewart
G S
GS
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D001435:Bacteriophages / Q000235:genetics*; D005516:Food Microbiology; D005814:Genes, Viral; D008156:Luciferases / Q000235:genetics*; D008460:Meat / Q000382:microbiology*; D013552:Swine; D013997:Time Factors
D008156:Luciferases