Pancreatic lipolytic enzymes in human duodenal contents. Radioimmunoassay compared with enzyme activity.
The total pancreatic lipolytic capacity was determined in duodenal contents in healthy humans 10-120 min after a liquid test meal, by estimating the amount of pancreatic lipase, colipase, carboxyl ester lipase, and phospholipase A2 by means of radioimmunoassays and enzymatic assays. The molar concentrations of the different proteins were of the same order of magnitude. The relative specific activity (enzyme activity/milligram immunoreactive protein expressed as a percentage of the specific activity of the respective pure protein) amounted to 75-120% for lipase, 45-80% for colipase, 30-70% for carboxyl ester lipase, and 45-120% for phospholipase A2. These varied, and sometimes low values can be explained by the fact that the enzymes are inhibited or partly inactivated in the duodenal contents by surface denaturation, in which cases the products are still immunoreactive. Also, the proforms of colipase and phospholipase A2 may not always be completely activated. Furthermore, the specific activities of the pure enzymes (and thus the relative specific activities) are related to the methods used, which are not specific enough to distinguish completely the three enzymes and the cofactor in duodenal contents.
Sternby
B
B
Dept. of Medical and Physiological Chemistry, University of Lund, Sweden.
Nilsson
A
A
Melin
T
T
Borgström
B
B
D000328:Adult; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000032:analysis; D004386:Duodenum / Q000201:enzymology*; D006801:Humans; D008049:Lipase / Q000032:analysis; D008875:Middle Aged; D010179:Pancreas / Q000201:enzymology*; D010741:Phospholipases A / Q000032:analysis; D054467:Phospholipases A2; D011863:Radioimmunoassay
D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; D008049:Lipase; D010741:Phospholipases A; D054467:Phospholipases A2