Optimal ratio of individual branched-chain amino acids in total parenteral nutrition of injured rats.
In this study, we investigated the optimal ratio of individual branched-chain amino acids (BCAA) in a balanced amino acid infusion in laparotomized rats. The total BCAA contents of four amino acid infusions were fixed at 31% of total amino acids. The weight ratios of individual BCAA (isoleucine:leucine:valine) in the solutions were 1:0.5:1, 1:1:1, 1:2:1, and 1:4:1, respectively. The laparotomized rats were infused with about 140 mg (experiment 1) and 100 mg (experiment 2) of nitrogen and 10 g of glucose daily for 7 days. In both experiments, no marked difference was observed in the mean cumulative 7-day nitrogen balance and the urinary 3-methyl-histidine levels of all the groups. The BCAA concentrations and the molar ratios of individual BCAA in plasma were disarranged by the infusion of the 1:0.5:1 and 1:4:1 solutions. The infusion of the 1:1:1 and 1:2:1 solutions tended, however, to allow the values to approach the preinfusion values. These results suggest that the optimal ratio of individual BCAA in an amino acid infusion lies between 1:1:1 and 1:2:1 for this injured rat model in total parenteral nutrition.
Iwasawa
Y
Y
Department of Biochemistry, Tanabe Seiyaku Co. Ltd., Osaka, Japan.
Kishi
T
T
Morita
M
M
Ikeda
K
K
Shima
H
H
Sato
T
T
D000596:Amino Acids / Q000008:administration & dosage / Q000097:blood / Q000652:urine; D000597:Amino Acids, Branched-Chain / Q000008:administration & dosage* / Q000097:blood / Q000652:urine; D000818:Animals; D007813:Laparotomy; D008297:Male; D009584:Nitrogen / Q000378:metabolism; D010289:Parenteral Nutrition, Total; D051381:Rats; D011919:Rats, Inbred Strains; D012996:Solutions; D015430:Weight Gain
D000596:Amino Acids; D000597:Amino Acids, Branched-Chain; D012996:Solutions; D009584:Nitrogen