title: Plasmid-encoded determinants for bacteriocin production and immunity in a Lactococcus lactis strain and purification of the inhibitory peptide.
abstract: Lactococcin, a bacteriocin produced by Lactococcus lactis subsp. lactis ADRIA 85LO30, was purified as a 2.3-2.4 kDa peptide. Six non-bacteriocin-producing (Bac-) and non-immune (Imm-) strains were isolated after curing experiments. These strains had in common the loss or modification of two plasmids: pOS4 (32 kb) and pOS5 (70 kb). By comparing pOS5 and several modified plasmids, a DNA region from pOS5 of about 10 kb, which was necessary for wild-type bacteriocin production and immunity, was identified.
authors.affiliation: URA 256, Laboratoire de Génétique Moléculaire, Université de Rennes, France.
authors.forename: A
authors.initials: A
authors.lastname: Dufour
authors.forename: D
authors.initials: D
authors.lastname: Thuault
authors.forename: A
authors.initials: A
authors.lastname: Boulliou
authors.forename: C M
authors.initials: CM
authors.lastname: Bourgeois
authors.forename: J P
authors.initials: JP
authors.lastname: Le Pennec
mesh_terms: D001430:Bacteriocins / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D005798:Genes, Bacterial; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010957:Plasmids
chemical_list: D001430:Bacteriocins; D004269:DNA, Bacterial; D004274:DNA, Recombinant; C069325:lactococcin A