title: ABO blood grouping of semen from mixed body fluids with monoclonal antibody to tissue-specific epitopes on seminal ABO blood group substance.
abstract: Practical methods were developed for ABO blood grouping of semen from mixed body fluids. An monoclonal antibody (P6-5H) which recognizes a tissue-specific epitope on a seminal ABO blood group substance (alpha 2-seminoglycoprotein) was used as a solid phase antibody for selective capture of the seminal ABO blood group substance. ABO blood group epitopes of secretor and non-secretor semen were detected in dilutions of 8 x 10(3)-3.2 x 10(4) and 8 x 10(3)-fold, respectively, by sandwich ELISA. ABO blood group epitopes were also detected in dilutions up to 4 x 10(3)-fold, irrespective of secretor status, by the sandwich absorption-elution test.
authors.affiliation: Department of Legal Medicine, Wakayama Medical College, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kimura
authors.forename: F
authors.initials: F
authors.lastname: Matsumura
authors.forename: K
authors.initials: K
authors.lastname: Sodesaki
authors.forename: M
authors.initials: M
authors.lastname: Osawa
authors.forename: H
authors.initials: H
authors.lastname: Ikeda
authors.forename: S
authors.initials: S
authors.lastname: Yasuda
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D001788:Blood Grouping and Crossmatching / Q000379:methods* / Q000592:standards; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005069:Evaluation Studies as Topic; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology*; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000276:immunology*; D012661:Semen / Q000276:immunology*; D029589:Seminal Plasma Proteins
chemical_list: D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D016056:Immunodominant Epitopes; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein