The gene encoding rat liver glycogen phosphorylase contains multiple polyadenylation signal sequences.
RNA blot analysis of rat liver and adipose tissues detected two glycogen phosphorylase (GP)-encoding transcripts. The polymerase chain reaction was used to characterize the 3'-noncoding region of the gene (L-GP) encoding liver-GP (L-GP) from the lean Zucker rat (Fa/Fa). Three distinct classes of colinear cDNA clones were identified by nucleotide (nt) sequence analysis, demonstrating that the L-GP gene contains at least three functional polyadenylation sites. The predominant L-GP transcript was generated by polyadenylation 130 nt 3' from the end of the coding region. A previously uncharacterized L-GP transcript is generated by polyadenylation at 346 nt 3' of the first polyadenylation site. Polyadenylation site selection does not appear to be regulated in a tissue-specific fashion. The relative steady-state L-GP mRNA levels in the different types of adipose tissues were comparable to, or exceeded transcript levels in liver.
Froman
B E
BE
Department of Neurology, University of California, Davis 95616.
Tait
R C
RC
Gorin
F A
FA
Horwitz
B A
BA
Stern
J S
JS
D000273:Adipose Tissue / Q000201:enzymology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D006005:Phosphorylases / Q000235:genetics*; D011061:Poly A / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D051381:Rats; D011924:Rats, Zucker; D015183:Restriction Mapping
D011061:Poly A; D006005:Phosphorylases