title: Recent advances in liquid chromatography-mass spectrometry and capillary zone electrophoresis-mass spectrometry for protein analysis.
abstract: The utility of the combination of separations techniques, such as liquid chromatography and capillary zone electrophoresis, with mass spectrometry in applications involving protein analysis is discussed. The use of continuous-flow fast atom bombardment and electrospray ionization mass spectrometry is compared for the analysis of tryptic digests. For liquid chromatography, both microbore and slurry-packed capillary bore columns were used to separate peptides from proteolytic digests.
authors.affiliation: Analytical Chemistry Center, University of Texas Medical School, Houston 77225.
authors.forename: M J
authors.initials: MJ
authors.lastname: Suter
authors.forename: B B
authors.initials: BB
authors.lastname: DaGue
authors.forename: W T
authors.initials: WT
authors.lastname: Moore
authors.forename: S N
authors.initials: SN
authors.lastname: Lin
authors.forename: R M
authors.initials: RM
authors.lastname: Caprioli
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004586:Electrophoresis / Q000379:methods*; D013006:Growth Hormone / Q000032:analysis; D006801:Humans; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014357:Trypsin
chemical_list: D010455:Peptides; D011506:Proteins; D013006:Growth Hormone; D014357:Trypsin