Preparation and storage of "leukocyte-depleted single donor" platelet concentrate: a step forward in effective haemotherapy.
"Leukocyte-depleted" platelet concentrates (LD-SDPC) were prepared by cotton-wool and by polyester filtration of "leukocyte-poor" PC collected by the elutriation technique. The storage characteristics of LD-SDPC were comparable to those of the filtered pools of multiple donor platelet concentrates (LD-MDPC). However, LD-SDPC were less activated and less damaged than LD-MDPC over a 7-day storage period, as evidenced by beta-thromboglobin percent release and lactic dehydrogenase percent leakage in both products. LD-SDPC were prophylactically transfused to 12 thrombocytopenic patients; the mean bleeding time was shortened from 12 min 20 sec to 4 min 39 sec. Corrected count increment (CCI) was 22.8 at 1 hr and 14.1 at 24 hr compared to 15.5 and 10.0, respectively, with standard PCs.
Elias
M K
MK
Red Cross Blood Bank Groningen-Drenthe, The Netherlands.
de Wolf
J T
JT
Blom
N
N
Rijskamp
L
L
Halie
R M
RM
Smit Sibinga
C T
CT
D016913:Blood Component Transfusion; D001792:Blood Platelets / Q000648:ultrastructure; D001793:Blood Preservation / Q000379:methods*; D002470:Cell Survival; D005374:Filtration; D003368:Gossypium; D006487:Hemostasis; D006801:Humans; D008212:Lymphocyte Depletion; D010974:Platelet Aggregation; D010976:Platelet Count; D017713:Platelet Transfusion; D010983:Plateletpheresis / Q000295:instrumentation; D011091:Polyesters; D013921:Thrombocytopenia / Q000628:therapy
D011091:Polyesters