title: [Polyphotobiotin--a new reagent for introducing biotin into nucleic acids].
abstract: A new reagent, polyphotobiotin (PPhB), for labelling DNA probes has been obtained using low molecule oligoethylenimine (M 600 Da). Photoreactive group of PPhB is 4-azido-salicylic acid. The procedure is simple and quick, the reaction time being about 20 min. PPhB-labelled DNA has the same efficiency in the hybridisation analysis as DNA labelled with Bio-4-dUTP via PCR.
authors.forename: M F
authors.initials: MF
authors.lastname: Turchinskiĭ
authors.forename: S N
authors.initials: SN
authors.lastname: Shcherbo
authors.forename: V D
authors.initials: VD
authors.lastname: Knorre
authors.forename: T B
authors.initials: TB
authors.lastname: Kolesnik
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Aleĭnik
mesh_terms: D000345:Affinity Labels; D001386:Azides / Q000737:chemistry*; D001435:Bacteriophages / Q000378:metabolism; D001710:Biotin / Q000031:analogs & derivatives* / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000345:Affinity Labels; D001386:Azides; D015342:DNA Probes; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D009696:Nucleic Acids; D011108:Polymers; D001710:Biotin; C045549:photobiotin
vernacular_title: Polifotobiotin--novyĭ reagent dlia vvedeniia biotina v nukleinovye kisloty.