Nontoxigenic strains of Clostridium difficile lack the genes for both toxin A and toxin B.
A total of 39 toxigenic and 20 nontoxigenic strains of Clostridium difficile were tested for the presence of either toxin A or toxin B by the polymerase chain reaction (PCR). All toxigenic strains produced cytotoxin as assayed by using highly sensitive fetal lung fibroblasts and were positive for toxin A as well as toxin B in the PCR assay. All nontoxigenic strains failed to produce toxin and were negative in the PCR assay. This study shows that nontoxigenic strains of Clostridium difficile lack the toxin A as well as the toxin B gene.
Fluit
A C
AC
Eijkman Winkler Laboratory for Medical Microbiology, University Hospital Utrecht, The Netherlands.
Wolfhagen
M J
MJ
Verdonk
G P
GP
Jansze
M
M
Torensma
R
R
Verhoef
J
J
D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D016360:Clostridioides difficile / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; C041348:tcdA protein, Clostridium difficile; C031815:toxB protein, Clostridium difficile