title: Yeast artificial chromosome-based genome mapping: some lessons from Xq24-q28.
abstract: Yeast artificial chromosomes (YACs) have recently provided a potential route to long-range coverage of complex genomes in contiguous cloned DNA. In a pilot project for 50 Mb (1.5% of the human genome), a variety of techniques have been applied to assemble Xq24-q28 YAC contigs up to 8 Mb in length and assess their quality. The results indicate the relative strength of several approaches and support the adequacy of YAC-based methods for mapping the human genome.
authors.affiliation: Department of Molecular Microbiology, Washington University School Medicine, St. Louis, Missouri 63110.
authors.forename: D
authors.initials: D
authors.lastname: Schlessinger
authors.forename: R D
authors.initials: RD
authors.lastname: Little
authors.forename: D
authors.initials: D
authors.lastname: Freije
authors.forename: F
authors.initials: F
authors.lastname: Abidi
authors.forename: I
authors.initials: I
authors.lastname: Zucchi
authors.forename: G
authors.initials: G
authors.lastname: Porta
authors.forename: G
authors.initials: G
authors.lastname: Pilia
authors.forename: R
authors.initials: R
authors.lastname: Nagaraja
authors.forename: S K
authors.initials: SK
authors.lastname: Johnson
authors.forename: J Y
authors.initials: JY
authors.lastname: Yoon
authors.forename: A
authors.initials: A
authors.lastname: Srivastava
authors.forename: J
authors.initials: J
authors.lastname: Kere
authors.forename: G
authors.initials: G
authors.lastname: Palmieri
authors.forename: A
authors.initials: A
authors.lastname: Ciccodicola
authors.forename: V
authors.initials: V
authors.lastname: Montanaro
authors.forename: G
authors.initials: G
authors.lastname: Romano
authors.forename: A
authors.initials: A
authors.lastname: Casamassimi
authors.forename: M
authors.initials: M
authors.lastname: D'Urso
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D015894:Genome, Human; D006801:Humans; D014960:X Chromosome