Determination of trifluorothymidine in the eye using high-performance liquid chromatography.
A high-performance liquid chromatographic assay for trifluorothymidine in biological matrices of the eye is presented. Sample pretreatment was based on liquid-liquid extraction with ethyl acetate. Following evaporation, the residue was analyzed using a reversed-phase octadecylsilane column with ultraviolet detection. The lower limit of detection is ca. 50 ng/ml (ca. 3 ng on column). The method is simple, reproducible and selective. Concentrations of trifluorothymidine in the aqueous humour, vitreous humour and cornea of normal rabbits after a 50-microliters topical dose of 1% trifluorothymidine are reported.
Riegel
M R
MR
Department of Ophthalmology, University of Colorado, School of Medicine, Denver 80262.
Ellis
P P
PP
D000818:Animals; D001082:Aqueous Humor / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003315:Cornea / Q000737:chemistry*; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet; D014271:Trifluridine / Q000032:analysis*
D014271:Trifluridine