Estimation of tyrosine by the attenuation of 4-iodophenol enhanced chemiluminescence.
Tyrosine markedly attenuates the chemiluminescence output intensity from the 4-iodophenol enhanced chemiluminescence assay system in a manner consistent with competition between the amino acid and luminol for the 4-iodophenoxy radical. This effect provides the basis for a sensitive assay of tyrosine. Interference by the other amino acids has been assessed; major interference by cysteine can be removed by incubation with iodoacetic acid.
Candy
T E
TE
Department of Chemistry, University of Newcastle upon Tyne, UK.
Mantle
D
D
Jones
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P
D002729:Chloromercuribenzoates; D007461:Iodoacetates; D019807:Iodoacetic Acid; D007462:Iodobenzenes / Q000737:chemistry / Q000494:pharmacology*; D008163:Luminescent Measurements; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds; D014443:Tyrosine / Q000032:analysis*; D020245:p-Chloromercuribenzoic Acid
D002729:Chloromercuribenzoates; D007461:Iodoacetates; D007462:Iodobenzenes; D013438:Sulfhydryl Compounds; D014443:Tyrosine; C050181:4-iodophenol; D020245:p-Chloromercuribenzoic Acid; D019807:Iodoacetic Acid