Contact activation factors in plasma from women using oral contraceptives--increased levels of factor XII, kinin-free high molecular weight kininogen and acetone-activated kallikrein.
The plasma levels of FXII, prekallikrein (PK) and high- and low molecular weight kininogens (HK and LK) were measured in women on low estrogen dose (30-40 micrograms ethinylestradiol) oral contraceptives (OC) and in controls. FXIIa was assayed in acetone-treated citrated plasma (CPL) with PK and the tetrapeptide S-2222 as substrates, and in acetone-treated citrated plasma with benzamidine (BPL) with PK as substrate. The level of FXII was found to be about 20% higher in OC-plasma than in control plasma. Kallikrein was assayed in CPL with S-2222 as substrate and in BPL with the tripeptide S-2302 as substrate. No difference in PK-level was observed in the CPL-based method, whereas an increase in kallikrein activity of about 30% was registered in BPL. The levels of HK and LK were estimated both in rocket immunoassay and in bioassay of released kinin. No difference in HK-level could be registered in immunoassay, whereas the bioassay revealed a HK-level in OC-plasma of 40% of the control level. Immunoblot studies showed that a substantial part of HK in OC-plasma was present as kinin-free protein (mol. wt. 103 KD), assumed to possess a higher cofactor potency than that of native HK. Both in bioassay and immunoassay the level of LK was found to be 60% higher in OC-plasma than in control plasma. Considered together the observations on contact factors made in this study provide support for the assumption of an increased readiness for contact activation in plasma from women using OC.
Hoem
N O
NO
Department of Pharmacology, University of Oslo, Norway.
Johannesen
S
S
Hauge
G
G
Rud
A C
AC
Sandem
S
S
Briseid
K
K
D000328:Adult; D003277:Contraceptives, Oral, Combined / Q000494:pharmacology*; D004789:Enzyme Activation; D004997:Ethinyl Estradiol / Q000494:pharmacology*; D005174:Factor XII / Q000032:analysis*; D005260:Female; D006801:Humans; D007704:Kininogens / Q000097:blood*; D011288:Prekallikrein / Q000032:analysis*
D003277:Contraceptives, Oral, Combined; D007704:Kininogens; D004997:Ethinyl Estradiol; D005174:Factor XII; D011288:Prekallikrein