Analysis of various antigens in golden hamster testis by monoclonal antibodies.
A total of 38 hybridomas producing monoclonal antibodies (mAbs) was established by immunizing BALB/c mice with extracts of the golden hamster testis. Six mAbs stained the acrosome of developing spermatids by immunofluorescence. Two mAbs (1A11 and 4D8) reacted with spermatid components other than acrosome. The mAbs 1C9 and 4D3 recognized a 103 kilodalton (kDa) protein on immunoblots, and were reactive to spermatocytes and early spermatids, but not to late spermatids and spermatozoa. This finding suggests that the protein functions for meiosis or early spermiogenesis. Four mAbs (3G2, 2E5, 2G3, and 3F10) stained all stages of spermatogenic cells. The remaining 24 mAbs showed a positive reaction to the basement membrane of the seminiferous tubule. Two of them, 3D6 and 3E5, recognized approximately 150 kDa major proteins, indicating that the antigen is an extracellular matrix.
Ohsako
S
S
Department of Veterinary Anatomy, Faculty of Agriculture, University of Tokyo, Japan.
Kurohmaru
M
M
Nishida
T
T
Hayashi
Y
Y
D000177:Acrosome / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D001324:Autoantigens / Q000737:chemistry / Q000276:immunology*; D001485:Basement Membrane / Q000276:immunology; D006224:Cricetinae; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D006825:Hybridomas; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D008647:Mesocricetus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D013087:Spermatids / Q000276:immunology; D013090:Spermatocytes / Q000276:immunology; D013093:Spermatogonia / Q000276:immunology; D013094:Spermatozoa / Q000276:immunology; D013737:Testis / Q000737:chemistry / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001324:Autoantigens