A rapid and efficient method of lysis of Listeria and other gram-positive bacteria using mutanolysin.
A rapid and simple procedure is described for cell lysis for preparation of nucleic acids and intact ribosomal RNA from Gram-positive bacteria. Commercial mutanolysin (purified from Streptomyces globisporus) was used for inducing lysis. Listeria, Lactobacillus and Lactococcus strains were very sensitive to mutanolysin when compared to lysozyme. Susceptibility to mutanolysin was improved by a preliminary treatment with acetone, and sodium dodecyl sulfate reduced the efficiency of lysis when used together with mutanolysin. The procedure was also effective for recovering plasmids from these bacteria.
Fliss
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Department of Food Science and Technology, Laval University, Québec, Canada.
Emond
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Simard
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Pandian
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D000096:Acetone / Q000494:pharmacology; D001433:Bacteriolysis; D004587:Electrophoresis, Agar Gel; D010450:Endopeptidases / Q000494:pharmacology*; D005821:Genetic Techniques; D005898:Glass; D006094:Gram-Positive Bacteria / Q000737:chemistry / Q000187:drug effects*; D008087:Listeria / Q000737:chemistry / Q000187:drug effects*; D009113:Muramidase / Q000378:metabolism; D009696:Nucleic Acids / Q000302:isolation & purification; D012335:RNA, Ribosomal / Q000302:isolation & purification
D009696:Nucleic Acids; D012335:RNA, Ribosomal; D000096:Acetone; D009113:Muramidase; D010450:Endopeptidases; C021816:mutanolysin