Subnanosecond single photon timing measurements using a pulsed diode-laser.
The convenient and inexpensive use of a pulsed diode-laser (Hamamatsu Photonics PLP-01 660 nm) is demonstrated as a low cost alternative to a standard pulsed laser or gas discharge flash system in a commercial time-correlated single photon counting instrument. Fluorescence lifetimes of compounds of photobiological interest such as phytochrome, chlorophyll a, 1,1'-diethyl-4,4' carbocyanine iodide (DCI/cryptocyanin),5,10,15,20-tetra(p-phenyl) porphyrin and stentorin I are presented using the pulsed diode-laser source.
Farrens
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Department of Chemistry, University of Nebraska-Lincoln 68588-0304.
Song
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D002734:Chlorophyll / Q000737:chemistry*; D000077194:Chlorophyll A; D007834:Lasers; D010834:Phytochrome / Q000737:chemistry*; D011827:Radiation; D011838:Radiation-Sensitizing Agents / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors
D011838:Radiation-Sensitizing Agents; D010834:Phytochrome; D002734:Chlorophyll; D000077194:Chlorophyll A