title: [Effective synthesis and cloning of the human interleukin-2 gene and its analog: expression of the interleukin-2 gene in E. coli cells].
abstract: Artificial DNA fragments encoding human interleukin-2 (133 a.a.) and its analogue (deletion of 14 C-terminal a.a.) were prepared by means of the DNA polymerase I mediated extension of synthetic polynucleotides having short overlapping sequences at their 3'-ends. The fragments were cloned in specially designed pFH-type plasmids and then excised by the FokI and other restriction endonucleases to yield the subfragments with the structurally predetermined 5'-unique cohesive ends. The complete synthetic gene was constructed by one or two-step ligation. The expressed IL-2 was tested by analysing the T-cell proliferation activity of E.coli crude lisates containing the pEXIL2 expression plasmid.
authors.forename: N K
authors.initials: NK
authors.lastname: Daniliuk
authors.forename: S V
authors.initials: SV
authors.lastname: Seregin
authors.forename: A N
authors.initials: AN
authors.lastname: Siniakov
authors.forename: O I
authors.initials: OI
authors.lastname: Serpinskiĭ
authors.forename: I N
authors.initials: IN
authors.lastname: Babkina
authors.forename: M A
authors.initials: MA
authors.lastname: Urmanova
authors.forename: V A
authors.initials: VA
authors.lastname: Riabinin
authors.forename: S G
authors.initials: SG
authors.lastname: Pozdniakov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D006801:Humans; D007376:Interleukin-2 / Q000138:chemical synthesis / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
chemical_list: D007376:Interleukin-2; D004247:DNA; D013936:Thymidine
vernacular_title: Effektivnyĭ sintez i klonirovanie gena interleĭkina-2 cheloveka i egoanaloga: ékspressiia v kletkakh E. coli gena interleĭkina-2.