title: Lack of compensatory megakaryocytopoiesis in HIV-1-seropositive thrombocytopenic individuals compared with immune thrombocytopenic purpura patients.
abstract: In this study we demonstrate that HIV-1-seropositive thrombocytopenic individuals, in contrast with immune thrombocytopenic purpura (ITP) patients, fail to have a compensatory increase of megakaryocytopoiesis. The in vitro growth of bone-marrow megakaryocyte progenitors (CFU-MK) and the production of granulocyte/macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-1 and IL-6 by bone-marrow mononuclear adherent cells and peripheral blood (PB) light-density mononuclear cells were studied in 12 HIV-1-seropositive thrombocytopenic individuals with respect to 12 ITP patients and 15 normal controls. In HIV-1-seropositive thrombocytopenic individuals, CFU-MK size (number of megakaryocytes per colony) was similar to normal controls but significantly lower (P less than 0.05) than in ITP patients. IL-1 and IL-6 production was similar in the three groups of subjects. On the other hand, GM-CSF production by bone-marrow mononuclear adherent cells in HIV-1-seropositive thrombocytopenic individuals was similar to normal controls but significantly (P less than 0.05) lower than in ITP patients, whereas GM-CSF production by PB light-density mononuclear cells was markedly (P less than 0.05) defective compared with both normal controls and ITP patients. The positive correlation between number and size of CFU-MK and production of GM-CSF by bone-marrow mononuclear adherent cells, observed in all three groups of subjects, demonstrates the central role of GM-CSF in the control of megakaryocytopoiesis.
authors.affiliation: Institute of Microbiology, University of Bologna, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Zauli
authors.forename: M C
authors.initials: MC
authors.lastname: Re
authors.forename: L
authors.initials: L
authors.lastname: Gugliotta
authors.forename: G
authors.initials: G
authors.lastname: Visani
authors.forename: N
authors.initials: N
authors.lastname: Vianelli
authors.forename: G
authors.initials: G
authors.lastname: Furlini
authors.forename: M
authors.initials: M
authors.lastname: La Placa
mesh_terms: D000328:Adult; D001853:Bone Marrow / Q000276:immunology / Q000473:pathology; D002448:Cell Adhesion; D003114:Colony-Forming Units Assay; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D006679:HIV Seropositivity / Q000150:complications* / Q000276:immunology / Q000473:pathology; D015497:HIV-1; D006410:Hematopoiesis; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000473:pathology; D008297:Male; D008533:Megakaryocytes / Q000473:pathology; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000473:pathology; D013921:Thrombocytopenia / Q000150:complications* / Q000473:pathology
chemical_list: D016207:Cytokines