Evidence for the existence of cytoskeleton-bound polysomes in plants.
When conventional, high ionic strength buffers were used for the isolation of polysomes from pea plants, less than 20% were retained in the detergent-insoluble pellet. Reducing Tris, K+ and Mg++ to 10 mM increased retention to 70%, and when a new, microfilament-stabilizing buffer was used, retention increased to 80%. Conditions which favoured polysome pelleting at lower g forces permitted the retention of actin in the pellet. The data are consistent with the hypothesis that higher plants, like animals, contain cytoskeleton-(actin)-bound polysomes.
Davies
E
E
School of Biological Sciences, University of Nebraska, Lincoln 68588-0118.
Fillingham
B D
BD
Oto
Y
Y
Abe
S
S
D003599:Cytoskeleton / Q000737:chemistry*; D007887:Fabaceae / Q000737:chemistry*; D008274:Magnesium; D010946:Plants, Medicinal; D011132:Polyribosomes / Q000737:chemistry*; D011188:Potassium; D014325:Tromethamine
D014325:Tromethamine; D008274:Magnesium; D011188:Potassium