The ste4+ gene, essential for sexual differentiation of Schizosaccharomyces pombe, encodes a protein with a leucine zipper motif.
Ste4- mutants of Schizosaccharomyces pombe are unable to undergo both mating and meiosis. We have cloned the ste4+ gene and its cDNA. The gene encodes a 264 amino acid protein with a typical leucine zipper motif homologous with the jun family. However, unlike the jun family, this protein does not have a typical basic region that precedes the leucine zipper. The transcription of this gene absolutely depends on the ste11+ gene and increases several fold upon nitrogen starvation, a general signal for sexual differentiation. Whereas ste4+ is essential for mating and meiosis, its overexpression inhibits these processes.
Okazaki
N
N
Department of Molecular Genetics, Osaka University, Japan.
Okazaki
K
K
Tanaka
K
K
Okayama
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D044387:GTP-Binding Protein beta Subunits; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D016758:Genes, jun / Q000235:genetics; D005816:Genetic Complementation Test; D020962:Heterotrimeric GTP-Binding Proteins; D016350:Leucine Zippers / Q000235:genetics*; D008540:Meiosis / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009584:Nitrogen / Q000494:pharmacology; D016755:Proto-Oncogene Proteins c-jun / Q000737:chemistry / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000235:genetics*
D044387:GTP-Binding Protein beta Subunits; D016755:Proto-Oncogene Proteins c-jun; D029701:Saccharomyces cerevisiae Proteins; C067576:Ste4 protein, S cerevisiae; D019204:GTP-Binding Proteins; D020962:Heterotrimeric GTP-Binding Proteins; D009584:Nitrogen