Cloning and expression of the lepidopteran toxin produced by Bacillus thuringiensis var Sotto in Escherichia coli.
During sporulation, Bacillus thuringiensis var. Sotto produces a parasporal crystalline protein which is toxic for the silk-worm, Bombyx mori and the cotton leaf worm, Spodoptera littoralis. The gene coding this crystal protein is present in a single plasmid. The plasmid DNA was isolated, purified and physically mapped using restriction endonuclease enzymes (R.E.). The gene coding the delta-endotoxin was inserted into Escherichia coli-Jm103, using cloning vector pUC8. Transformed E. coli cells were found to synthesize the delta-endotoxin as demonstrated by the pathogenicity of the transformed cells against 4th instar larvae of S. littoralis.
Rady
M H
MH
Ain-Shams University, Faculty of Science, Entomology Dept.
D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics*; D003001:Cloning, Molecular; D004731:Endotoxins / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D004926:Escherichia coli; D005069:Evaluation Studies as Topic; D015870:Gene Expression; D007300:Insect Control; D007915:Lepidoptera; D010957:Plasmids
D004731:Endotoxins