A DNA sequence for the discrimination of Neisseria gonorrhoeae from other Neisseria species.
A 350 base pair Neisseria gonorrhoeae DNA restriction fragment was cloned after subtractive hybridization to Neisseria meningitidis DNA. This restriction fragment hybridized to 105 out of 106 N. gonorrhoeae strains tested. While three N. meningitidis strains did not hybridize to this probe, Neisseria mucosa DNA exhibited cross-hybridization. This particular clone was used to screen a N. gonorrhoeae genomic DNA library. A positive 2.4 kilobase pair clone was shown by DNA sequencing to contain two long open reading frames. One open reading frame did not hybridize to N. mucosa and other Neisseria species, while it retained specificity for the original 105 N gonorrhoeae strains. This open reading frame also showed significant homology to cytosine DNA methyltransferases.
Miyada
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CG
Syva Company, Palo Alto, California 94303.
Born
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TL
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D015257:DNA-Cytosine Methylases / Q000235:genetics; D015723:Gene Library; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000235:genetics* / Q000302:isolation & purification; D009345:Neisseria meningitidis / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D016364:Reading Frames; D015183:Restriction Mapping
D004269:DNA, Bacterial; D015347:RNA Probes; D015257:DNA-Cytosine Methylases