A cell cycle ts mutant, tsJT16, is defective in p70 synthesis through protein kinase C-dependent and -independent pathways.
tsJT16 is a G0/G1 ts mutant from the Fischer rat fibroblast line. It has a ts defect in a function operating early after growth stimulation with fetal bovine serum (FBS). A primarily induced gene product, p70, was not synthesized at 40 degrees C after stimulation with serum, while c-fos and c-myc mRNAs accumulated under the same condition. This paper reports that p70 was synthesized following stimulation of G0-arrested cells with platelet-derived growth factor, epidermal growth factor (EGF), and 12-0-tetradecanoylphorbol-13-acetate (TPA) at 34 degrees C, but not at 40 degrees C. However, it was synthesized at both temperatures after addition of A23187. In protein kinase C-deprived cells, peptide growth factors and A23187 induced p70 at 34 degrees C, whereas TPA did not. Fibroblast growth factor and insulin did not induce p70. Induction of c-fos and c-myc occurred at both temperatures after the stimulation with FBS, TPA or A23187. These results indicated that the defect in tsJT16 to induce p70 is likely to be located at the common downstream of protein kinase C-dependent and -independent pathways, but is independent from the pathway of calcium mobilization.
Miyake
E
E
Department of Cellular and Molecular Biology, Hiroshima University School of Medicine, Japan.
Takasuka
T
T
Sakayama
M
M
Ide
T
T
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics*; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005347:Fibroblasts / Q000502:physiology; D016193:G1 Phase; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics*; D016762:Genes, fos; D016259:Genes, myc; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011493:Protein Kinase C; D011494:Protein Kinases / Q000235:genetics*; D051381:Rats; D016192:Resting Phase, Cell Cycle; D019893:Ribosomal Protein S6 Kinases; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D009687:Nuclear Proteins; D010982:Platelet-Derived Growth Factor; D004815:Epidermal Growth Factor; D011494:Protein Kinases; D019893:Ribosomal Protein S6 Kinases; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate