A cell-free culture system for development of large numbers of Brugia pahangi larvae.
Techniques used in the in vitro culture of massive numbers of Brugia pahangi third-stage larvae (L3) are described. Procedures for larval preparation and four culture conditions, with or without animal cell co-cultures, were studied, resulting in the adoption of a relatively simple cell-free culture system for routine harvesting of larval moulting excretory/secretory product.
Hong
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XQ
Department of Veterinary Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
Wong
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MM
D000818:Animals; D002009:Brugia / Q000254:growth & development*; D003470:Culture Media; D007814:Larva / Q000254:growth & development
D003470:Culture Media