title: A simple purification method of squeezed krill for obtaining high levels of hydrolytic enzymes.
abstract: The raw material originating from freshly caught Antarctic krill (E. superba) was processed by centrifugation and then immediately frozen. This squeezed fluid adjusted to pH 7 was subjected to spontaneous autolysis by storing at different temperatures (20-45 degrees C) and times (10-48 h). The autolyzed material resulted in 3 distinct phases after high speed centrifugation. The intermediate phase (water-phase) was collected and concentrated by membrane filtration. The high-molecular-weight substances (greater than 10,000 Daltons) were further purified by ion-exchange chromatography and eluted by a salt gradient. In this matter a bulk enzymatic material from krill can be efficiently obtained for further purification of different hydrolytic enzymes (peptide hydrolases/carbohydrases).
authors.affiliation: Department of Biochemistry, Kabi Pharmacia Therapeutics, Uppsala, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Karlstam
authors.forename: J
authors.initials: J
authors.lastname: Vincent
authors.forename: B
authors.initials: B
authors.lastname: Johansson
authors.forename: C
authors.initials: C
authors.lastname: Brynö
mesh_terms: D000818:Animals; D002268:Carboxypeptidases / Q000302:isolation & purification; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003445:Crustacea / Q000201:enzymology*; D005615:Freezing; D005966:Glucuronidase / Q000302:isolation & purification; D006821:Hyaluronoglucosaminidase / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000302:isolation & purification*; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000302:isolation & purification; D013053:Spectrophotometry; D013696:Temperature; D014357:Trypsin / Q000302:isolation & purification
chemical_list: D006867:Hydrolases; D005966:Glucuronidase; D006821:Hyaluronoglucosaminidase; D002268:Carboxypeptidases; D010447:Peptide Hydrolases; D014357:Trypsin