Direct observation of the biphasic conformational change of DNA induced by cationic polymers.
The interaction between T4 DNA and basic polypeptides was observed using fluorescence microscopy. Free DNA molecules exhibited random Brownian motion accompanying the conformational change. With the addition of polycation, such as histone and polyarginine, DNA molecules tended to shrink to become spherical shapes. The persistent lengths and the distributions of long axis lengths of DNA-polyarginine complexes were determined from the video images at various polyarginine concentrations. It is demonstrated that the conformation of DNA changes in a biphasic manner in the presence of polyarginine.
Minagawa
K
K
College of General Education, Nagoya University, Japan.
Matsuzawa
Y
Y
Yoshikawa
K
K
Matsumoto
M
M
Doi
M
M
D002412:Cations; D004247:DNA / Q000737:chemistry* / Q000187:drug effects; D004279:DNA, Viral / Q000737:chemistry* / Q000187:drug effects; D007700:Kinetics; D008856:Microscopy, Fluorescence; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000494:pharmacology*; D012996:Solutions; D013604:T-Phages
D002412:Cations; D004279:DNA, Viral; D010455:Peptides; D012996:Solutions; C015462:polyarginine; D004247:DNA