Application of the computerized compensation method and the orthogonal functions method to correct for irrelevant interference in spectrofluorometry.
The computerized compensation method and the orthogonal functions method were applied to correct for the presence of interferences in spectrofluorometric analysis. The methods were demonstrated by the determination of tetracycline (TC) and anhydrotetracycline (ATC) in combination. The calibration graphs were linear in the concentration range 0.02-0.12 mg/100 ml of either TC or ATC.
Barary
M H
MH
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Alexandria University, Egypt.
D002138:Calibration; D008432:Mathematical Computing; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013752:Tetracycline / Q000032:analysis
D013752:Tetracycline