title: Development of an in vitro microtest to assess drug susceptibility of Babesia bovis and Babesia bigemina.
abstract: Continuous cultivation of the bovine hemoparasites Babesia bovis and Babesia bigemina was developed as an in vitro microtest to assess parasite susceptibility to babesicidal compounds. Reproducibility of parasite multiplication rates was independent of culture size, making it possible to use a microscale of 100 microliters for each test sample. Inhibitory concentrations (IC50s) of a commonly used babesicide, quinuronium sulfate, evaluated by this in vitro method were found to be 5 x 10(-8) g/ml for B. bovis and 2 x 10(-9) g/ml for B. bigemina.
authors.affiliation: Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: C R
authors.initials: CR
authors.lastname: Brockelman
authors.forename: P
authors.initials: P
authors.lastname: Tan-ariya
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D001403:Babesia / Q000187:drug effects* / Q000254:growth & development; D016793:Babesia bovis / Q000187:drug effects* / Q000254:growth & development; D011806:Quinolinium Compounds / Q000494:pharmacology*; D014508:Urea / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000981:Antiprotozoal Agents; D011806:Quinolinium Compounds; D014508:Urea; C009154:quinuronium sulfate