title: A Bacillus subtilis dipeptide transport system expressed early during sporulation.
abstract: Two previously identified Bacillus subtilis DNA segments, dciA and dciB, whose transcripts accumulate very rapidly after induction of sporulation, were found in the same 6.2 kb transcription unit, now known as the dciA operon. Analysis of the sequence of the dciA operon showed that its putative products are homologous to bacterial peptide transport systems. The product of the fifth gene, DciAE, is similar to peptide-binding proteins from Escherichia coli and Salmonella typhimurium (DppA and OppA) and B. subtilis (OppA). A null mutation in dciAE abolished the ability of a proline auxotroph to grow in a medium containing the dipeptide Pro-Gly as sole proline source, suggesting that the dciA operon encodes a dipeptide transport system.
authors.affiliation: Department of Molecular Biology and Microbiology, Tufts University Health Sciences Campus, Boston, Massachusetts 02111.
authors.forename: C
authors.initials: C
authors.lastname: Mathiopoulos
authors.forename: J P
authors.initials: JP
authors.lastname: Mueller
authors.forename: F J
authors.initials: FJ
authors.lastname: Slack
authors.forename: C G
authors.initials: CG
authors.lastname: Murphy
authors.forename: S
authors.initials: S
authors.lastname: Patankar
authors.forename: G
authors.initials: G
authors.lastname: Bukusoglu
authors.forename: A L
authors.initials: AL
authors.lastname: Sonenshein
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D001426:Bacterial Proteins; D001483:Base Sequence; D001693:Biological Transport, Active / Q000235:genetics; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000235:genetics; D004151:Dipeptides / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression / Q000502:physiology; D007700:Kinetics; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009842:Oligopeptides / Q000378:metabolism; D009876:Operon / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012486:Salmonella typhimurium / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013171:Spores, Bacterial / Q000235:genetics* / Q000378:metabolism / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004151:Dipeptides; D026901:Membrane Transport Proteins; D009842:Oligopeptides; C037230:oligopeptide permease, Bacteria; C057749:prolylglycine; C049251:prolyl-glycyl-glycine