Separation of living red blood cells by gravitational field-flow fractionation.
The field-flow fractionation technique, using the earth's gravitational field, has been applied to peripheral blood cell populations. A more or less symmetrical, gaussian-like, elution peak is generally observed for the red cell population. The bimodal cell population obtained after a massive transfusion is shown to result in a shoulder on the red blood cell elution profile. In one case where a similar shouldering peak was obtained from a non-transfused donor, the existence of an immunological double population has been demonstrated. This suggests that field-flow fractionation has some potential for complementary biomedical diagnosis.
Cardot
P J
PJ
Laboratoire de Chimie Analytique et d'Electrochimie Organiques, Université Paris XI, Faculté de Pharmacie, Châtenay-Malabry, France.
Gerota
J
J
Martin
M
M
D002469:Cell Separation / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology*; D006112:Gravitation; D006801:Humans