Elimination of caffeine interference in high-performance liquid chromatographic determination of cotinine in human plasma.
A high-performance liquid chromatographic method with ultraviolet photometric detection for the determination of cotinine in human plasma was described. The use of a 30-cm reversed-phase column and of a mobile phase consisting of water-methanol-0.1 M sodium acetate-acetonitrile (72:21:5.6:1.4, v/v), pH 4.1, eliminated caffeine interference. A simplified solid-phase extraction procedure was also performed for plasma samples.
Pichini
S
S
Clinical Biochemistry Department, Istituto Superiore di Sanità, Rome, Italy.
Altieri
I
I
Pacifici
R
R
Rosa
M
M
Zuccaro
P
P
D002110:Caffeine / Q000032:analysis / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003367:Cotinine / Q000032:analysis / Q000097:blood*; D004347:Drug Interactions; D006801:Humans
D002110:Caffeine; D003367:Cotinine