Direct interfacing of high-speed countercurrent chromatography to frit electron ionization, chemical ionization, and fast atom bombardment mass spectrometry.
Direct interfacing of analytical high-speed countercurrent chromatography (HSCCC) to mass spectrometry (MS) was demonstrated for the first time, and its performance was evaluated in terms of chromatography and mass spectrometry. HSCCC/MS interface was based upon Frit electron ionization (EI), chemical ionization (CI), and fast atom bombardment (FAB). Separations were conducted by newly developed HSCCC-4000 with a 2.5-cm revolutional radius and 0.3 mm or 0.55 mm i.d. multilayer coiled column which is capable of operating at a maximum speed of 4000 rpm. To demonstrate the potential capability of HSCCC/frit MS, three indole auxin mixtures, two mycinamicin (macrolide antibiotics) mixtures, and a colistin complex (peptide antibiotics) were analyzed under HSCCC/frit EI, CI, and FABMS conditions, respectively. The data obtained indicated that interfacing to frit/MS does not adversely affect the chromatographic resolution and mass spectra provide structural information. The HSCCC system interfaced with a frit-equipped mass spectrometer will offer a new dimension in the separation of biologically important substances.
Oka
H
H
Aichi Prefectural Institute of Public Health, Nagoya, Japan.
Ikai
Y
Y
Kawamura
N
N
Hayakawa
J
J
Harada
K
K
Murata
H
H
Suzuki
M
M
Ito
Y
Y
D002845:Chromatography / Q000295:instrumentation*; D004583:Electrons; D007211:Indoles / Q000032:analysis; D007477:Ions; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000295:instrumentation*
D007211:Indoles; D007477:Ions