N-Trifluoroacetyl-ethanolamine: a proposed urinary metabolite of halothane: validation and measurement in children.
It has been postulated that trifluoroacetyl chloride, a halothane metabolite, can bind covalently with the phosphatidylethanolamine component of the hepatic cell membrane and cause cell necrosis. Breakdown of the necrotic hepatocyte would release N-trifluoroacetyl-ethanolamine (TFAE) into the serum with subsequent urinary excretion. An original High Performance Liquid Chromatography (HPLC) method for the measurement of TFAE is described. In six children 1% halothane was administered for one hour and the halothane uptake measured. Urinary excretion of TFAE was measured for up to eight days and found to be 0.09 +/- 0.07% or less of the absorbed halothane. In children TFAE is not a major urinary metabolite of halothane.
Wark
H
H
Department of Anaesthesia, Royal Alexandra Hospital for Children, Camperdown, N.S.W., Australia.
Earl
J
J
Chau
D
D
Overton
J
J
D000769:Anesthesia, Inhalation; D002648:Child; D002675:Child, Preschool; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004983:Ethanolamines / Q000032:analysis / Q000138:chemical synthesis / Q000652:urine*; D006221:Halothane / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007223:Infant; D010084:Oxidation-Reduction; D012119:Respiration; D013997:Time Factors
D004983:Ethanolamines; C011135:N-trifluoroacetyl-2-aminoethanol; D006221:Halothane