Cloning of a sequence of Aquaspirillum magnetotacticum that complements the aroD gene of Escherichia coli.
A 2 kb DNA fragment isolated from a cosmid library of Aquaspirillum magnetotacticum strain MS-1 complements the aromatic-metabolite requirements and iron-uptake deficiencies of Escherichia coli and Salmonella typhimurium strains that lack a functional aroD (biosynthetic dehydrodquinase) sequence. All recombinant cosmids selected for their aroD complementation property carry this sequence. No DNA sequence homology has, however, been detected by Southern hybridization between the cloned fragment and the aroD gene of E. coli or the qa2 (catabolic dehydroquinase) gene of Neurospora crassa.
Berson
A E
AE
Department of Molecular and Cell Biology, SRI International, Menlo Park, California 94025.
Hudson
D V
DV
Waleh
N S
NS
D003001:Cloning, Molecular; D003360:Cosmids / Q000235:genetics; D003594:Cytoplasmic Granules; D004926:Escherichia coli / Q000235:genetics*; D005290:Ferric Compounds / Q000378:metabolism*; D015723:Gene Library; D005816:Genetic Complementation Test; D006090:Gram-Negative Bacteria / Q000235:genetics*; D006836:Hydro-Lyases / Q000235:genetics*; D008280:Magnetics; D015183:Restriction Mapping
D005290:Ferric Compounds; D006836:Hydro-Lyases; C020000:3-dehydroquinate dehydratase