[Synthesis of hexamethyl propylamine oxime and comparative study of its diastereoisomers].
This is a report on the synthesis of hexamethyl-propylene-amine oxime (HM-PAO), a new agent for regional cerebral blood flow (gamma CBF) imaging, the separation of its diastereoisomers (dl- and meso-HM-PAO), and a comparative study of three forms of Tc- 99m labelled HM-PAO (dl-, meso- and mixture-) in chromatography, mice biodistribution and rabbit imaging. The results showed that the Rf value of Tc-99m-dl-HM-PAO (0.8-1.0) on silica G thin layer chromatography with MEK as solvent was different from that of Tc-99m-meso-HM-PAO (0.6-0.8), and that the retention of dl- isomer in the brain was higher and more static than those of meso-and mixture HM-PAO but identical with Ceretec, a dl-HM-PAO kit manufactured by Amersham Inc., England. The primary clinical applications for gamma CBF SPECT imaging demonstrated a satisfactory result.
Tan
T
T
Wang
D
D
Kuang
A
A
He
L
L
Xu
P
P
D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging; D002560:Cerebrovascular Circulation; D006801:Humans; D051379:Mice; D015609:Organotechnetium Compounds / Q000138:chemical synthesis*; D010091:Oximes / Q000138:chemical synthesis*; D011817:Rabbits; D013237:Stereoisomerism; D019690:Technetium Tc 99m Exametazime; D015899:Tomography, Emission-Computed, Single-Photon
D015609:Organotechnetium Compounds; D010091:Oximes; C068296:hexamethylpropyleneamine oxime; D019690:Technetium Tc 99m Exametazime