Co2+ and Ni2+ resistance in Saccharomyces cerevisiae associated with a reduction in the accumulation of Mg2+.
A mutant strain of Saccharomyces cerevisiae (NR 6), which can be associated with a reduction in the accumulation of Mg2+ has been isolated. This mutant strain displays resistance to both Ni2+ and Co2+, but not, however, towards Cu2+, Cd2+, Mn2+, Zn2+ and Cr2O7(2-). Both Co2+ and Ni2+ uptake by the mutant strain is less than by the wild type (CMR-50). The inhibitory effects of Ni2+ and Co2+ on the growth of both strains NR-6 and CMR-50 can be cancelled by increasing the concentrations of Mg2+ in the medium and to a lower extent by the addition of Ca2+ which results in a decreased uptake of these metals. It therefore appears that the resistant mechanisms of this mutant strain NR-6 is due to a reduction in the uptake of Co2+ and Ni2+ via a Mg2+ transport system.
Joho
M
M
Department of Biology, Faculty of Science, Ehime University, Matsuyama, Japan.
Tarumi
K
K
Inouhe
M
M
Tohoyama
H
H
Murayama
T
T
D001692:Biological Transport; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D003035:Cobalt / Q000378:metabolism / Q000494:pharmacology*; D003470:Culture Media; D004347:Drug Interactions; D004352:Drug Resistance, Microbial; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology; D008670:Metals / Q000378:metabolism / Q000494:pharmacology; D009154:Mutation; D009532:Nickel / Q000378:metabolism / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development
D003470:Culture Media; D008670:Metals; D003035:Cobalt; D009532:Nickel; D008274:Magnesium; D002118:Calcium