The complete amino-acid sequence of the heavy chain of the human myeloma protein WIE, an immunoglobulin D.
The human myeloma protein WIE is a lambda-type immunoglobulin D; the amino-acid sequence of its Fc part and aminoethylated heavy chain was completely determined. The VH-part (subgroup III) begins N-terminally with 5-oxoproline, and it contains a long, unique CDR3 region. Since the constant part differs from known delta chains by one amino-acid substitution in the hinge region, IgD WIE probably represents an allotypic variant. As in other protein delta chains, O-glycosylations are confined to the hinge region. Furthermore, the ratios of N-glycosylations at the three positions are identical in IgD WAH [Takahashi, N. et al. (1984) J. Chromatogr. 317, 11-26.] and IgD WIE (100%, 50%, 100%). From the most conserved constant domain, C delta 3, a three-dimensional model was constructed to clarify the role of its delta-specific substitutions and glycosylation.
Friedrich
R J
RJ
Max-Planck-Institut für Experimentelle Medizin, Abteilung Immunchemie, Göttingen.
Bätge
R
R
Schranner
I
I
Kotucha
U
U
Eckart
K
K
Hilschmann
N
N
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D006801:Humans; D007122:Immunoelectrophoresis; D007072:Immunoglobulin D / Q000737:chemistry / Q000235:genetics*; D007128:Immunoglobulin Fragments / Q000302:isolation & purification; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009194:Myeloma Proteins / Q000737:chemistry / Q000235:genetics*; D011487:Protein Conformation; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D016339:Spectrometry, Mass, Fast Atom Bombardment
D007072:Immunoglobulin D; D007128:Immunoglobulin Fragments; D007143:Immunoglobulin Heavy Chains; D009194:Myeloma Proteins; C072624:myeloma protein WIE