title: Clinical usefulness of serum tartrate-resistant acid phosphatase activity determination to evaluate bone turnover.
abstract: The study was carried out to evaluate the clinical validity and usefulness of serum tartrate-resistant acid phosphatase (TRAP) activity determined using an improved spectrophotometric assay. Enzyme activity was measured in 84 normal subjects and in 109 patients with common metabolic bone diseases. Mean values of serum TRAP activity in male subjects (n = 19; 10.4 +/- 2.15 U l-1) were not significantly different from those found in female subjects (n = 65; 10.8 +/- 1.8 U l-1). In the latter group mean values were significantly raised in post-menopausal subjects (10.5 +/- 2.0 U l-1; p less than 0.01) compared with mean values in pre-menopausal women (8.45 +/- 1.8 U l-1). We found a significant inverse correlation between serum TRAP activity values and bone mineral density (BMD) measured both at an ultradistal radial point (n = 33, r = -0.506; p less than 0.01), and at the lumbar spine (n = 57, r = -0.261; p less than 0.05). Mean serum TRAP activity values in patients with metabolic bone diseases were: primary hyperparathyroidism, n = 30: 14.2 +/- 4.89 U l-1, p less than 0.001 vs normal subjects; chronic maintenance haemodialysis, n = 19: 17.4 +/- 6.7, p less than 0.001; metastatic cancer, n = 13: 21.2 +/- 6.3, p less than 0.001; post-surgical hypoparathyroidism, n = 10: 9.9 +/- 1.8, NS; involutional osteoporosis, n = 20: 12.5 +/- 2.3 p less than 0.001; Paget's disease, n = 10: 16.8 +/- 3.5, p less than 0.001; osteomalacia, n = 7: 19.5 +/- 3.31, p less than 0.001.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Servizio Aggregato Malattie del Ricambio Minerale, University of Rome La Sapienza, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Scarnecchia
authors.forename: S
authors.initials: S
authors.lastname: Minisola
authors.forename: M T
authors.initials: MT
authors.lastname: Pacitti
authors.forename: V
authors.initials: V
authors.lastname: Carnevale
authors.forename: E
authors.initials: E
authors.lastname: Romagnoli
authors.forename: R
authors.initials: R
authors.lastname: Rosso
authors.forename: G F
authors.initials: GF
authors.lastname: Mazzuoli
mesh_terms: D000135:Acid Phosphatase / Q000097:blood*; D000328:Adult; D000375:Aging / Q000378:metabolism; D015519:Bone Density / Q000502:physiology*; D001851:Bone Diseases, Metabolic / Q000201:enzymology / Q000503:physiopathology*; D016723:Bone Remodeling; D005260:Female; D006801:Humans; D006961:Hyperparathyroidism / Q000201:enzymology / Q000503:physiopathology; D008297:Male; D008593:Menopause; D008875:Middle Aged; D010001:Osteitis Deformans / Q000201:enzymology / Q000503:physiopathology; D010018:Osteomalacia / Q000201:enzymology / Q000503:physiopathology; D010024:Osteoporosis / Q000201:enzymology / Q000503:physiopathology; D015663:Osteoporosis, Postmenopausal / Q000201:enzymology / Q000503:physiopathology; D013053:Spectrophotometry; D013644:Tartrates / Q000494:pharmacology
chemical_list: D013644:Tartrates; D000135:Acid Phosphatase