Application of cryo-sapphire knife in rapid frozen ultrathin sectioning for electron microscopy.
Conventional sectioning of frozen and fixed tissues is usually performed using the glass knife. Although the glass knife is economical, it has several disadvantages. In this study, the Authors have examined the sectioning quality of the sapphire knife in frozen tissues as an alternative to the glass knife. It was concluded that the sapphire knife has better quality and durability than the conventional glass knife in cutting frozen tissues.
Suzuki
K
K
Central Institute for Electron Microscopic Researches, Nippon Medical School, Tokyo, Japan.
Aihara
K
K
Hanaichi
T
T
Takagi
I
I
Sato
T
T
D000818:Animals; D016616:Cryoultramicrotomy / Q000295:instrumentation* / Q000379:methods; D005629:Frozen Sections; D006801:Humans; D007422:Intestines / Q000648:ultrastructure; D008099:Liver / Q000648:ultrastructure; D051381:Rats; D012272:Ribs / Q000648:ultrastructure