[Mechanism of damage to erythrocytes after burn injury in rat--changes in lipid peroxidation, antioxidant function and sulfhydryl groups].
Thermal burn on the back of rat (20% TBSA full-thickness burn) was used to study the changes in free radicals and lipid peroxidation and function of the antioxidation of erythrocytes postburn. It was found that there were increase in the concentration of lipid peroxides and decrease in activity of glutathione peroxidase (GSHpx) in plasma. The activity of RBC GSHpx increased significantly on day 1 postburn. When RBC GSHpx could not resist the injury, the function of antioxidation decreased, such as the activities of G6PD, GSHpx and the concentration of GSH and NPSH. RBC LPO increased, which cross-linked with sulfhydryl proteins and inflicted injury to RBC membrane. It might be concluded that the mechanism of damage of RBC after burn injury is due to chain reactions of free radicals and lipid peroxidation of the cell membrane. It also proved that there were changes in functional capability of antioxidation in vivo after burn injury. Further study is needed to locate the site of production of free radicals within or/and in the neighborhood of RBC and elucidate the transfer of the messages which cause the change.
Meng
X M
XM
Dept of Public Health, Third Military Medical College, Chongqing.
D000818:Animals; D002056:Burns / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005609:Free Radicals; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D005609:Free Radicals; D013438:Sulfhydryl Compounds; D008315:Malondialdehyde; D005954:Glucosephosphate Dehydrogenase; D005979:Glutathione Peroxidase