Differential effects of propentofylline on the production of cytokines by peripheral blood mononuclear cells in vitro.
The effects of the xanthine derivative propentofylline on the production of interleukin-6 (IL-6), interleukin-1 beta (IL-1 beta), and tumor necrosis factor-alpha (TNF-alpha) by human peripheral blood mononuclear cells (PBMCs) were studied. When PBMCs were cultured with propentofylline in vitro, the production of IL-6 was markedly increased at concentrations of 0.1 to 3.0 mmol/L of propentofylline and the production of IL-1 beta was slightly increased at concentrations of 1.0 to 3.0 mmol/L. However, an insignificant increase in TNF-alpha production was observed. When the effects of propentofylline on the production of IL-6, IL-1 beta, and TNF-alpha by OK-432-stimulated PBMCs were examined, IL-6 secretion was not significantly increased, whereas production of IL-1 beta and TNF-alpha were significantly suppressed in a dose-dependent manner. The results demonstrate that propentofylline has a differential effect on the production of IL-6, IL-1 beta, and TNF-alpha by PBMCs, and it is proposed that propentofylline may exert pharmacologic actions on the regulation of the production of cytokines in the central nervous system.
Miki
S
S
Third Department of Internal Medicine, Nissei Hospital, Osaka, Japan.
Miki
Y
Y
D000328:Adult; D016207:Cytokines / Q000096:biosynthesis* / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014970:Xanthines / Q000494:pharmacology*
D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D014970:Xanthines; C032114:propentofylline