Proteoglycan synthesis in cultures of murine retinal neurons and photoreceptors.
1. In recent years, a number of histochemical and immunocytochemical studies have suggested that proteoglycans, particularly those in the interphotoreceptor matrix, exhibit altered distributions in several murine models for retinal degenerations. We are using a cell culture system to characterize the proteoglycans synthesized by neurons and photoreceptors derived from mouse retina, with the long-term goal of analyzing their role in retinal degenerations. 2. In this study we describe initial studies using cells derived from the retinas of normal mice. Cultures of retinal neurons and photoreceptors, which were free of glial, epithelia, or endothelial cells, were labeled with 3H-glucosamine and 35SO4. Proteoglycans isolated from the medium and cell layer were analyzed on the basis of charge, relative hydrodynamic size, and glycosaminoglycan content. 3. The studies indicate that the cultures actively synthesize proteoglycans. The medium contained predominantly chondroitin sulfate/dermatan sulfate, while the cell layer had a higher proportion of heparan sulfate, indicating a differential distribution between the two compartments.
Murillo-Lopez
F
F
Retinal Degenerations Research Center, Wilmer Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Politi
L
L
Adler
R
R
Hewitt
A T
AT
D000818:Animals; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005260:Female; D006025:Glycosaminoglycans / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism*; D010786:Photoreceptor Cells / Q000378:metabolism*; D011509:Proteoglycans / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D012160:Retina / Q000378:metabolism*
D006025:Glycosaminoglycans; D011509:Proteoglycans