title: Signal-to-noise optimization of HPLC-fluorometric systems and their application to the analysis of indoles.
abstract: The signal-to-noise optimization of high performance liquid chromatographic (HPLC) flow-cell fluorometric systems is described and the possibilities for further improving limits of detection for indoles is discussed. Application of HPLC-fluorometry to analyses in rat cerebrospinal fluid (CSF), human lumbar CSF, and human blood is presented. Finally, the intriguing clinical finding of hyperserotonemia in autism is discussed.
authors.affiliation: Child Study Center, Yale University School of Medicine, New Haven, Connecticut 06510.
authors.forename: G M
authors.initials: GM
authors.lastname: Anderson
mesh_terms: D000818:Animals; D001321:Autistic Disorder / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D005470:Fluorometry / Q000295:instrumentation*; D006801:Humans; D007211:Indoles / Q000032:analysis*; D051381:Rats; D014364:Tryptophan / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid
chemical_list: D007211:Indoles; D014364:Tryptophan