Sequence homology of androgen-regulated epididymal proteins with glutathione peroxidase in mice.
The M53 cDNA for Mr 24,000 androgen-regulated secretory proteins of the mouse caput epididymidis previously reported has been sequenced. This clone presents a 5'-incomplete open reading frame of 525 base pairs. The 3'-untranslated region of 946 base pairs contains a repetitive DNA element of the rodent B1 family just between two canonical polyadenylation signals AATAAA, upstream of the poly(A) track. The deduced amino acid sequence for the Mr 24,000 proteins reveals significant homologies of 66.6, 66, 65.2, 63.1 and 64.5% with mouse, rat, man, bull and rabbit cloned selenium-dependent glutathione peroxidases, respectively. The present results emphasize previous studies performed in numerous laboratories suggesting that the major protective system against oxidative damage in mouse spermatozoa could be an enzyme similar to glutathione peroxidase.
Ghyselinck
N B
NB
Laboratoire de Biologie Cellulaire, Université Blaise Pascal et CNRS URA 360, Aubière, France.
Jimenez
C
C
Dufaure
J P
JP
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D004270:DNA, Circular / Q000235:genetics*; D030501:Epididymal Secretory Proteins; D005979:Glutathione Peroxidase / Q000235:genetics*; D006801:Humans; D008297:Male; D008667:Metalloproteins / Q000235:genetics*; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D051381:Rats; D012643:Selenium / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013735:Testicular Hormones / Q000235:genetics*
D004270:DNA, Circular; D030501:Epididymal Secretory Proteins; D008667:Metalloproteins; D012333:RNA, Messenger; D013735:Testicular Hormones; D005979:Glutathione Peroxidase; D012643:Selenium