title: Calretinin, a neuronal calcium binding protein, inhibits phosphorylation of a 39 kDa synaptic membrane protein from rat brain cerebral cortex.
abstract: The neuronal calcium binding protein calretinin was studied for possible effects on brain protein phosphorylation. Calretinin (100 nM) inhibited the appearance of a calcium stimulated 39 kDa phosphoprotein within a synaptic membrane fraction following sucrose density centrifugation. Calmodulin or a specific protein kinase C inhibitor had no effect on either the phosphorylation of the 39 kDa protein or the inhibition produced by calretinin. At the same concentration, calretinin produced a slight increase in the phosphorylation of several other synaptic membrane proteins which appeared additive with the stimulation produced by either calmodulin or phosphatidylserine in the presence of calcium.
authors.affiliation: Laboratory of Clinical Science, NIMH, Bethesda, MD 20892.
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: L
authors.initials: L
authors.lastname: Winsky
authors.forename: D M
authors.initials: DM
authors.lastname: Jacobowitz
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D064032:Calbindin 2; D002147:Calmodulin / Q000494:pharmacology; D002540:Cerebral Cortex / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism* / Q000494:pharmacology; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D051381:Rats; D064030:S100 Calcium Binding Protein G / Q000494:pharmacology*; D013570:Synaptic Membranes / Q000378:metabolism*
chemical_list: C576512:Calb2 protein, rat; D064032:Calbindin 2; D002147:Calmodulin; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; D064030:S100 Calcium Binding Protein G; D000255:Adenosine Triphosphate