[The separation of true coagulases from other enzymes of the extracellular proteolytic complex of Aspergillus ochraceus by using affinity chromatography].
A complex of proteases has been isolated from the cultural broth of fungi Aspergillus ochraceus HP-19 by precipitation ammonium sulfate. Using the method of affinity chromatography on the biospecific sorbents the conditions for separation of proteases into ones with coagulase activity and with fibrinolytic activity have been found.
Cherdyntseva
T A
TA
Egorov
N S
NS
D001235:Aspergillus ochraceus / Q000201:enzymology*; D001777:Blood Coagulation / Q000187:drug effects; D002846:Chromatography, Affinity / Q000379:methods; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D003030:Coagulase / Q000302:isolation & purification* / Q000494:pharmacology; D020689:Exopeptidases; D066298:In Vitro Techniques; D010447:Peptide Hydrolases / Q000302:isolation & purification*; D011516:Prothrombin / Q000187:drug effects
D003030:Coagulase; D011516:Prothrombin; D020689:Exopeptidases; D010447:Peptide Hydrolases
Otdelenie istinnykh koagulaz or drugikh fermentov vnekletochnogo proteoliticheskogo kompleksa Aspergillus ochraceus s pomoshch'iu affinnoĭ khromatografii.