title: Effect of glucose on the expression of c-myc gene in cultured RINm5F cell.
abstract: The study was designed to examine the effect of glucose on the expression of c-myc gene in cultured RINm5F cells. After monolayer culture was established in RPMI 1640 media supplemented with 10% fetal calf serum (FCS), the cells were cultured in various concentrations of glucose and 1 or 10% FCS for another 24 hours. A mRNA was extracted from the cultured cells by a single step method, and Northern analysis was done to detect RNA band. A 0.5 kilobase single band was detected as c-myc mRNA. The expression of c-myc gene mRNA was reduced with increased concentration of glucose with 1% FCS. However, supplementation of 10% FCS abolished the effect of glucose on expression of c-myc gene. These findings suggested that glucose in conjunction with other growth promoting factors played an important role in expression of oncogene and cell growth in RINm5F cells.
authors.affiliation: Department of Internal Medicine, Kyung Hee University School of Medicine, Seoul, Korea.
authors.forename: S W
authors.initials: SW
authors.lastname: Kim
authors.forename: I M
authors.initials: IM
authors.lastname: Yang
authors.forename: J W
authors.initials: JW
authors.lastname: Kim
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
authors.forename: K W
authors.initials: KW
authors.lastname: Kim
authors.forename: Y K
authors.initials: YK
authors.lastname: Choi
authors.forename: K S
authors.initials: KS
authors.lastname: Suh
mesh_terms: D000818:Animals; D002460:Cell Line; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D016259:Genes, myc / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology*; D007340:Insulinoma / Q000235:genetics*; D010190:Pancreatic Neoplasms / Q000235:genetics*; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D005947:Glucose