Characterization and quantification of anti-T in human serum using a reliable hemolysis test.
The need of fresh-frozen donor plasma with a low level of anti-T has been emphasized recently. Anti-T, as administered by transfusion of fresh-frozen plasma, has been accused repeatedly of enhancing hemolysis in septic children with T transformation of red cells. Therefore, a new hemolysis test for the quantification of anti-T in human serum has been developed. With our test, anti-T-poor plasma donors can be found. Additional results raise substantial doubt as to the pathogenetic role of anti-T in the development of the hemolytic-uremic syndrome, found in septic children with red-cell T transformation. It is impossible to predict in vivo hemolysis induced by anti-T knowing the temperature characteristics and the ionic conditions causing this antibody to mediate hemolysis in vitro. Obviously, T transformation itself plays the major pathogenetic role in these patients, and not the presence of anti-T. In the case of disseminated intravascular coagulation, a content of anti-T cannot be construed as prohibiting transfusion of fresh-frozen plasma to such patients.
Lynen
R
R
Abteilung Transfusionsmedizin, Klinikum der Universität, Göttingen, BRD.
Simson
G
G
Rumpf
K W
KW
Neumeyer
H
H
D000906:Antibodies / Q000032:analysis* / Q000302:isolation & purification; D000957:Antigens, Viral, Tumor / Q000276:immunology*; D001681:Biological Assay; D004912:Erythrocytes / Q000276:immunology*; D006463:Hemolytic-Uremic Syndrome / Q000276:immunology; D006801:Humans; D037102:Lectins; D009996:Osmotic Fragility; D013696:Temperature
D000906:Antibodies; D000957:Antigens, Viral, Tumor; D037102:Lectins