Dystrophin: a sensitive and reliable immunochemical assay in tissue and cell culture homogenates.
A modified polyacrylamide gel electrophoresis system is described which provides excellent resolution of very high molecular weight proteins. This system has been successfully applied to the immunochemical detection of dystrophin in mouse and rat skeletal muscle, mouse myotubes in cell culture, and in human muscle-biopsy specimens. The mass of total homogenate protein (3-12 micrograms) and the relative quantity of dystrophin detected immunologically were found to be strongly correlated (r = 0.970 - 0.995). The method described here requires minute quantities of tissue or cells to accurately evaluate the relative amount of dystrophin present. The entire procedure for the detection of dystrophin is simple, rapid and cost efficient compared to other available techniques.
Ho-Kim
M A
MA
Laval University Hospital Research Center, Ste-Foy, Québec, Canada.
Bédard
A
A
Vincent
M
M
Rogers
P A
PA
D000818:Animals; D001706:Biopsy; D002478:Cells, Cultured; D016189:Dystrophin / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D009132:Muscles / Q000737:chemistry* / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains
D016189:Dystrophin