Autoantibodies to serum proteins in women with recurrent spontaneous abortion: subsequent pregnancy outcome.
Sera from 130 first trimester pregnant women were tested for their serum antibody level against a naturally occurring serum antigen purified from non-pregnancy sera. IgG and IgM antibody level was measured using enzyme linked immunosorbent assay. Results indicate that patients with multiple abortion (n = 26) have significantly (p = 0.0029) lower level of IgG antibody and significantly (p = 0.0001) higher level of IgM antibodies against the serum antigen as compared to the patients with successful pregnancies with no history of miscarriage (n = 63). Western blot analysis identified the serum antigen recognized by the IgM antibody as a 24 kDa molecular mass component. These IgG and IgM antibodies may play an important role in the outcome of pregnancy.
Bose
R
R
Department of Obstetrics Gynecology and Reproductive Sciences, University of Manitoba, Winnipeg, Canada.
Yang
M
M
Pan
N
N
D000026:Abortion, Habitual / Q000276:immunology*; D000917:Antibody Formation; D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens / Q000276:immunology / Q000302:isolation & purification; D001798:Blood Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis / Q000276:immunology; D011247:Pregnancy; D011256:Pregnancy Outcome; D011257:Pregnancy Proteins / Q000276:immunology; D011379:Prognosis
D001323:Autoantibodies; D001324:Autoantigens; D001798:Blood Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011257:Pregnancy Proteins