Study of biodistribution of enflurane in rats with in vivo 19F MRI.
In vivo 19F magnetic resonance imaging (MRI) of anesthetized rats enabled us to visualize the biodistribution of fluorinated anesthetics and to document the changes in MR signals in the body during the induction and the elimination phase of anesthesia. The authors examined in vivo 19F MRI in rats anesthetized with concentrations of 1.75-2.0% enflurane and demonstrated its in vivo distribution with concomitant 1H and 13C MRI to verify the anatomical correlation. Distinct 19F MR signals were acquired predominantly from the systemic adipose tissue and the liver. Additionally, the temporal changes in the tissue during and after anesthesia were characterized with in vivo 19F MRI in 6.4 min of the acquisition time. The 19F MR signals increased with time after anesthesia; however, the signals from the adipose tissue were apparently stronger than those from the liver. Following the discontinuation of inhalation, the MR signals in the liver decreased far more rapidly than those from the adipose tissue. When the animal woke up and began to move, the MR signals were still visible in the adipose tissue. These results confirmed the fact that enflurane dissolves preferentially in the adipose tissue and remains when the anesthetic effect disappears. Additionally, 19F MR signals of the liver during the elimination phase might reflect the concentration of enflurane in the blood.
Hashimoto
T
T
Division of Clinical Sciences, National Institute of Radiological Sciences, Chiba-shi, Japan.
Ikehira
H
H
Fukuda
H
H
Ueshima
Y
Y
Tateno
Y
Y
D000005:Abdomen; D000273:Adipose Tissue / Q000378:metabolism*; D000769:Anesthesia, Inhalation; D000818:Animals; D002244:Carbon; D004737:Enflurane / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005461:Fluorine; D006859:Hydrogen; D007089:Image Enhancement; D007091:Image Processing, Computer-Assisted; D008099:Liver / Q000378:metabolism*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012469:Salivary Glands / Q000378:metabolism; D013909:Thorax / Q000378:metabolism; D013997:Time Factors
D005461:Fluorine; D002244:Carbon; D006859:Hydrogen; D004737:Enflurane