The plasmacytoma J558L lacks constitutively active NF-kappa B and is deficient in early response gene activation.
In mature B cells the nuclear factor NF-kappa B which binds within the kappa enhancer is constitutively present in the nucleus. However, the lambda light chain producing myeloma J558L has been found to lack constitutively functional NF-kappa B. Deoxycholate released functional NF-kappa B from cytoplasmic extracts and functional NF-kappa B was present in J558L following cycloheximide but not phorbol ester treatment. J558L was also unable to respond to phorbol ester stimulation with synthesis of mRNA from the early response gene TIS11. J558L differs from S107, another myeloma which was found to be deficient in the synthesis of NF-kappa B but not in the activation of TIS11. Somatic cell hybrids were used to further define the defect in J558L; hybrids were made with the myelomas S107 and S194 and the pre-B cell line 70Z/3. In general, complementation of the defect in J558L was observed; however there was not a direct correlation between the levels of TIS11 mRNA and NF-kappa B expression in the somatic cell hybrids, suggesting that the pathways of activation of these genes, while possibly sharing common elements, are not identical. The defect in J558L was surprising given that it has frequently been used for the expression of transfected light chain genes.
Anderson
M T
MT
Department of Microbiology, Columbia University, New York, NY.
Wims
L A
LA
Morrison
S L
SL
D015152:Blotting, Northern; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D002855:Chromatography, Thin Layer; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000235:genetics*; D005816:Genetic Complementation Test; D007147:Immunoglobulin Light Chains / Q000235:genetics; D008745:Methylation; D016328:NF-kappa B / Q000235:genetics*; D010954:Plasmacytoma / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
D007147:Immunoglobulin Light Chains; D016328:NF-kappa B; D015500:Chloramphenicol O-Acetyltransferase; D013755:Tetradecanoylphorbol Acetate