title: Muramyl peptide-binding sites are located inside target cells.
abstract: Using flow cytometry and fluorescence polarization analysis, specific muramyl peptide-binding sites were shown to be located inside T-lymphocytes, macrophages and neuroblastoma cells, but not inside B-cells. No binding sites were found on the cell surface. The number of binding sites for each cell type was determined. Two types of binding sites were observed for myelomonocytic WEHI-3 cells with Kd values of 21 and 540 nM. Inhibition analysis demonstrated that for effective binding, an intact glycopeptide molecule and D-configuration of isoglutamine residue are important.
authors.affiliation: Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.
authors.forename: M V
authors.initials: MV
authors.lastname: Sumaroka
authors.forename: I S
authors.initials: IS
authors.lastname: Litvinov
authors.forename: S V
authors.initials: SV
authors.lastname: Khaidukov
authors.forename: T N
authors.initials: TN
authors.lastname: Golovina
authors.forename: M V
authors.initials: MV
authors.lastname: Kamraz
authors.forename: R L
authors.initials: RL
authors.lastname: Komal'eva
authors.forename: T M
authors.initials: TM
authors.lastname: Andronova
authors.forename: E A
authors.initials: EA
authors.lastname: Makarov
authors.forename: V A
authors.initials: VA
authors.lastname: Nesmeyanov
authors.forename: V T
authors.initials: VT
authors.lastname: Ivanov
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D001665:Binding Sites; D001667:Binding, Competitive; D002240:Carbohydrate Sequence; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D005456:Fluorescent Dyes; D006020:Glycopeptides / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D006020:Glycopeptides; C072334:fluoresceinthiocarbamoyl-Lys-GlcNAc-1-4-MurNAc-Ala-isoglutamine