Isolation and characterization of a species-specific DNA probe for Candida albicans.
As a model for the isolation of species-specific sequences of DNA, we isolated and characterized a species-specific DNA fragment from the Candida albicans genome. We used a series of differential colony hybridization experiments, in which a C. albicans genomic library was hybridized with genomic DNA probes from related organisms to minimize the number of potentially specific C. albicans DNA fragments to be tested. Six clones were tested by dot blot analysis, and one of them, a 1348 bp EcoRI DNA fragment, was confirmed as specific for C. albicans. This species-specific fragment could be utilized as a DNA probe for rapid, sensitive, and specific diagnosis of C. albicans DNA in clinical specimens.
Oren
I
I
Department of Medicine, Wayne State University, Detroit, MI 48201.
Manavathu
E K
EK
Lerner
S A
SA
D001483:Base Sequence; D015139:Blotting, Southern; D002176:Candida albicans / Q000235:genetics*; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D015342:DNA Probes / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D015723:Gene Library; D008969:Molecular Sequence Data; D013045:Species Specificity
D003062:Codon; D015342:DNA Probes; D004271:DNA, Fungal