[The use of the Soviet detergents dezintegron-O and dezintegron-B for the solubilization of influenza virus glycoproteins].
Native detergents, desintegron-O-and desintegron-B, solubilize hemagglutinins of the influenza virus as intensively, as foreign drugs (mulgophen, zwittergent and sodium sarcozyl) do. This permits recommending desintegrons for quantitative determination of hemagglutinin of the influenza virus in virus-containing materials by means of the reaction of single radial immunodiffusion. Desintegron-O and desintegron-B permit extracting enzymatically active neuraminidase from influenza A viruses with 44.6-83.4% yield.
Krivorutchenko
Iu L
IuL
Krivoshein
Iu S
IuS
Andronovskaia
I B
IB
Kastrikina
L N
LN
Lonskaia
N I
NI
Rud'ko
A P
AP
D003902:Detergents / Q000494:pharmacology*; D006389:Hemagglutinins, Viral / Q000302:isolation & purification; D005779:Immunodiffusion; D009980:Influenza A virus / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D009439:Neuraminidase / Q000187:drug effects / Q000302:isolation & purification; D012995:Solubility; D012997:Solvents / Q000494:pharmacology*; D014760:Viral Fusion Proteins / Q000187:drug effects* / Q000302:isolation & purification
D003902:Detergents; D006389:Hemagglutinins, Viral; D012997:Solvents; D014760:Viral Fusion Proteins; D009439:Neuraminidase
Ispol'zovanie otechestvennykh detergentov dezintegrona-O i dezintegrona-B dlia soliubilizatsii glikoproteidov virusa grippa.