Vanadate activates pentose phosphate pathway and glycolysis, and raises fructose 2,6-bisphosphate concentration in slices of lactating rat mammary gland.
In mammary gland slices from lactating rats, vanadate increased the rate of glucose oxidation via the pentose phosphate pathway by 36% and raised the glucose flux via glycolysis by 47%. Furthermore, vanadate increased the fructose 2,6-bisphosphate (Fru-2,6-P2) level by 33%. The effect of vanadate on glucose oxidation was compared to the effect of insulin. The present data indicate that 0.5mM vanadate has an effect on glucose utilization similar to that of insulin but does not reach the same level.
Sochor
M
M
Department of Biochemistry, University College and Middlesex School of Medicine, London.
Kunjara
S
S
Sumathy
K
K
el Hassan
T
T
Greenbaum
A L
AL
D000818:Animals; D005260:Female; D005635:Fructosediphosphates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects*; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007774:Lactation; D008055:Lipids / Q000096:biosynthesis; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism*; D010427:Pentose Phosphate Pathway / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D014638:Vanadates / Q000494:pharmacology*
D005635:Fructosediphosphates; D007328:Insulin; D008055:Lipids; D014638:Vanadates; C027652:fructose 2,6-diphosphate; D005947:Glucose