title: High amounts of beta-endorphin in peripheral blood mononuclear cells from HANE patients.
abstract: We measured peripheral blood mononuclear cells (PBMC) beta-endorphin (BE) in patients suffering from hereditary angioneurotic edema (HANE), a disease attributed to C1-esterase inhibitor (C1INH) deficiency inherited as an autosomal dominant trait. Two orders of considerations prompted us to undertake the study reported herein: the presence of elevated plasma BE concentrations in HANE and the demonstration of BE-immunoreactivity in human unstimulated peripheral blood leukocytes obtained from healthy volunteers. Our results show that patients suffering from HANE have a very high BE presence in uncultured, unstimulated PBMC and in unstimulated PBMC cultured for 48 h. At this time high BE concentrations are detected in the culture supernatants. These observations suggest that in HANE patients the same factor(s) involved in causing increased secretion and release of BE from the pituitary (and, in turn, increased plasma BE levels) can play a relevant role also in the determination of high BE presence in PBMC and BE release from the cells.
authors.affiliation: Dipartimento Chirurgico, II University of Rome Tor Vergata, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Perricone
authors.forename: A E
authors.initials: AE
authors.lastname: Panerai
authors.forename: P
authors.initials: P
authors.lastname: Sacerdote
authors.forename: C
authors.initials: C
authors.lastname: Moretti
authors.forename: N
authors.initials: N
authors.lastname: Pasetto
authors.forename: C
authors.initials: C
authors.lastname: de Carolis
authors.forename: G
authors.initials: G
authors.lastname: de Sanctis
authors.forename: L
authors.initials: L
authors.lastname: Fontana
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000799:Angioedema / Q000097:blood*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D008875:Middle Aged; D001615:beta-Endorphin / Q000097:blood*
chemical_list: D001615:beta-Endorphin