Histamine is a transient marker of small intestinal injury induced by luminal acetic acid and casein.
We compared the time course of histamine release with other markers of intestinal injury in a rabbit model of necrotizing enterocolitis. Injury was induced by luminal acetic acid (200 mM) and casein (10 mg/ml) and experiments terminated after 45 min or 3 hr. Compared to saline controls there was a significant elevation of epithelial permeability (51Cr-EDTA clearance) and luminal protein levels at both time points. Luminal fluid histamine levels were approximately 120-fold greater than saline controls at 45 min but were indistinguishable from control values at 3 hr. We conclude that although mast cell activation is a characteristic of this model, elevations in histamine levels are transient.
Miller
M J
MJ
Department of Pediatrics, Louisiana State University School of Medicine, New Orleans 70112-2822.
Zhang
X J
XJ
Gu
X
X
Sadowska-Krowicka
H
H
Clark
D A
DA
D000085:Acetates; D019342:Acetic Acid; D000818:Animals; D015415:Biomarkers; D002364:Caseins; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005122:Exudates and Transudates / Q000378:metabolism; D006632:Histamine / Q000032:analysis*; D007413:Intestinal Mucosa / Q000378:metabolism; D007419:Intestinal Secretions / Q000737:chemistry; D007421:Intestine, Small / Q000473:pathology*; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D009336:Necrosis; D010539:Permeability; D011506:Proteins / Q000378:metabolism; D011817:Rabbits
D000085:Acetates; D015415:Biomarkers; D002364:Caseins; D011506:Proteins; D006632:Histamine; D019342:Acetic Acid