title: The effect of insulin and relaxin upon mitosis (in vitro) in mammary tissue from pregnant and lactating pigs.
abstract: The rate of cellular proliferation in the mammary glands of pigs during late gestation and lactation was assessed by measuring the incorporation of 3H-thymidine (T1) into the DNA of mammary gland explants in vitro. The T1 showed a linear response over the first 9 hr in vitro, and was not affected by the addition of 500 ng insulin/ml medium. From day 100 to parturition the T1 rose, reached a peak at 2 d after parturition and declined during lactation to the lowest levels seen at day 21 of lactation. The inclusion of 0-1000 ng relaxin/ml medium on T1 at 24-72 hr in vitro had no effect in stimulating T1 in mammary tissue explants taken from either pregnant or lactating pigs.
authors.affiliation: AFRC Institute of Grassland and Environmental Research, HURLEY, Maidenhead, Berks, UK.
authors.forename: H L
authors.initials: HL
authors.lastname: Buttle
authors.forename: C L
authors.initials: CL
authors.lastname: Lin
mesh_terms: D000818:Animals; D003470:Culture Media; D046508:Culture Techniques; D005260:Female; D007328:Insulin / Q000494:pharmacology*; D007774:Lactation / Q000187:drug effects / Q000502:physiology; D008321:Mammary Glands, Animal / Q000187:drug effects* / Q000254:growth & development; D008938:Mitosis / Q000187:drug effects; D011247:Pregnancy; D012065:Relaxin / Q000494:pharmacology*; D012098:Reproduction / Q000187:drug effects* / Q000502:physiology; D013552:Swine / Q000254:growth & development*
chemical_list: D003470:Culture Media; D007328:Insulin; D012065:Relaxin