title: Determination of trifluorothymidine in the eye using high-performance liquid chromatography.
abstract: A high-performance liquid chromatographic assay for trifluorothymidine in biological matrices of the eye is presented. Sample pretreatment was based on liquid-liquid extraction with ethyl acetate. Following evaporation, the residue was analyzed using a reversed-phase octadecylsilane column with ultraviolet detection. The lower limit of detection is ca. 50 ng/ml (ca. 3 ng on column). The method is simple, reproducible and selective. Concentrations of trifluorothymidine in the aqueous humour, vitreous humour and cornea of normal rabbits after a 50-microliters topical dose of 1% trifluorothymidine are reported.
authors.affiliation: Department of Ophthalmology, University of Colorado, School of Medicine, Denver 80262.
authors.forename: M R
authors.initials: MR
authors.lastname: Riegel
authors.forename: P P
authors.initials: PP
authors.lastname: Ellis
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003315:Cornea / Q000737:chemistry*; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet; D014271:Trifluridine / Q000032:analysis*
chemical_list: D014271:Trifluridine