title: Microbial metabolism of quinoline and related compounds. XII. Isolation and characterization of the quinoline oxidoreductase from Rhodococcus spec. B1 compared with the quinoline oxidoreductase from Pseudomonas putida 86.
abstract: Quinoline oxidoreductase from Rhodococcus spec. B1 was purified 39-fold to apparent homogeneity in a 5-step procedure with a recovery of 26%. The Mr of the native enzyme as determined by gel chromatography was 300,000. SDS polyacrylamide gel electrophoresis of the enzyme revealed 3 protein bands corresponding to Mr 82,000, 32,000, and 18,000. The enzyme contains 1.3 atoms of molybdenum, 8 atoms of iron, 8 atoms of acid-labile sulphur, 2 molecules of FAD and 2 molecules of molybdopterin cytosine dinucleotide. Cyanide, 4-hydroxymercuribenzoate and methanol were effective as inhibitors. The amino-terminal protein sequences of the 3 subunits of quinoline oxidoreductase from Rhodococcus B1 compared to those of quinoline oxidoreductase from Pseudomonas putida 86 revealed no difference among 71 amino acids examined.
authors.affiliation: Institut für Mikrobiologie, Universität Hohenheim.
authors.forename: B
authors.initials: B
authors.lastname: Peschke
authors.forename: F
authors.initials: F
authors.lastname: Lingens
mesh_terms: D000595:Amino Acid Sequence; D002384:Catalysis; D007501:Iron / Q000032:analysis; D008969:Molecular Sequence Data; D008982:Molybdenum / Q000032:analysis; D010088:Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D016958:Pseudomonas putida / Q000201:enzymology* / Q000378:metabolism; D011804:Quinolines / Q000378:metabolism*; D012240:Rhodococcus / Q000201:enzymology* / Q000378:metabolism; D014871:Water Microbiology
chemical_list: D011804:Quinolines; D008982:Molybdenum; D007501:Iron; C037219:quinoline; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C068667:quinoline 2-oxidoreductase