Determination of several retinoids, carotenoids and E vitamers by high-performance liquid chromatography. Application to plasma and tissues of rats fed a diet rich in either beta-carotene or canthaxanthin.
A method, using two different systems, is described for the high-performance liquid chromatographic analysis of retinol, retinal, retinoic acid, retinyl acetate, retinyl palmitate, alpha-, beta- and gamma-carotene, beta-apo-6'-, beta-apo-8', beta-apo-10'- and beta-apo-12'-carotenal, ethyl beta-apo-8'-carotenoate, alpha-tocopherol and alpha-tocopheryl acetate. The first system consists of a laboratory-packed Hypersil-ODS 3-microns column and a mobile phase of acetonitrile-methylene chloride-methanol-water (70:10:15:5, v/v). The second system consists of a laboratory-packed Hypersil-ODS 3-microns column and a mobile phase of acetonitrile-methylene chloride-methanol-water (70:10:15:5, v/v). The second system consists of a laboratory-packed Nucleosil C18 3-microns column and a mobile phase of acetonitrile-0.1 M ammonium acetate (80:20, v/v). The detection limits in standard solutions were 10 ng/ml for retinoids and carotenoids and 60 ng/ml for the E vitamers. Analysis of the tissues and plasma of rats, after 2 weeks on a diet supplemented with either beta-carotene or canthaxanthin (both 2 mg/g), led to the conclusion that the rats were able both to transport and store beta-carotene and canthaxanthin and to convert beta-carotene to retinol. Incubation of cytosol preparations from the mucosa of the small intestine of rat with 1 microgram of beta-carotene resulted in the formation of 10-20 ng of retinal within 1 h.
Van Vliet
T
T
Department of Experimental Biology, TNO Toxicology and Nutrition Institute, Zeist, The Netherlands.
Van Schaik
F
F
Van Schoonhoven
J
J
Schrijver
J
J
D000818:Animals; D016644:Canthaxanthin / Q000008:administration & dosage*; D002338:Carotenoids / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D005260:Female; D007421:Intestine, Small / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012176:Retinoids / Q000097:blood / Q000378:metabolism*; D014810:Vitamin E / Q000097:blood / Q000378:metabolism*; D019207:beta Carotene
D012176:Retinoids; D019207:beta Carotene; D014810:Vitamin E; D002338:Carotenoids; D016644:Canthaxanthin