[Isolation of a histone-specific proteinase from brain cell nuclei of rats].
A method for isolating histone proteinase of rat brain chromatin is described, including ammonium sulphate fractionation gel filtration on sefacryl 5200, ion exchange chromatography on mono S and affinity chromatography with benzamidine sefarose. The enzyme molecular weight equals 25 kDa. It is purified 15621 times in comparison with initial nuclear extract.
Protas
A F
AF
Chaialo
P P
PP
D000818:Animals; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D002467:Cell Nucleus / Q000201:enzymology*; D010450:Endopeptidases / Q000302:isolation & purification*; D008297:Male; D008970:Molecular Weight; D051381:Rats; D011916:Rats, Inbred F344; D013379:Substrate Specificity
D010450:Endopeptidases; C021361:histone proteases
Vydelenie giston-spetsifichesoĭ proteinazy iz iader kletok mozga krys.