A simple method of separation of Pneumocystis carinii from rat lung.
Existing methods for purification of Pneumocystis carinii are unsatisfactory. Dialysis is a simple, non-stressful treatment which aided purification of organisms from rat cells. Yields were quantitated using a Petroff-Hausser chamber and nuclear staining. Following dialysis overnight, three times the yields of similar, undialysed preparations were obtained, with less than 0.2% contamination by lung cells. Overnight incubation at 4 degrees C also improved separation, but yields were only half those following dialysis. Dialysis is now used routinely in the preparation of pure Pneumocystis carinii suspensions.
Read
J A
JA
Regional Virus Laboratory, City Hospital, Edinburgh, UK.
Burns
S M
SM
D000818:Animals; D001431:Bacteriological Techniques; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D003956:Dialysis; D005260:Female; D008168:Lung / Q000382:microbiology*; D008297:Male; D011010:Pneumocystis / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000382:microbiology*; D051381:Rats; D011919:Rats, Inbred Strains