A major Litomosoides carinii microfilarial sheath glycoprotein (gp22): amino terminal sequence and immunological studies with corresponding synthetic peptides.
The major glycoprotein of the sheath of Litomosoides carinii microfilariae (gp22) was analysed for its amino acid and amino sugar composition. It is rich in proline, glutamine/glutamic acid and glycine and contains (N-acetyl)galactosamine. The N-terminal amino acid sequence was determined up to position 37. It consists of a group of 6 repeats of the pentapeptide sequence methionine-glycine-proline-glutamine-proline with two minor modifications in repeats 3-6, while the first two repeats follow the general pattern more loosely. Identical N-terminal amino acid sequences were found in at least two other sheath polypeptides (33 kDa, 39 kDa). Antisera prepared against 3 overlapping synthetic peptides corresponding to the amino terminus of gp22 recognized different epitopes. They all reacted with identical patterns of sheath polypeptides. The antisera failed to recognize antigens of 4th-stage larvae of L. carinii. In contrast, cross-reacting epitopes were detected in other parasite stages. Antisera reacted with material surrounding embryos and microfilariae in the uterus of females, and caused patchy fluorescence on the sheath of blood-derived and in vitro-released microfilariae.
Bardehle
G
G
Biochemisches Institut am Klinikum, Justus-Liebig-Universität, Giessen, FRG.
Conraths
F J
FJ
Fahrenholz
F
F
Hintz
M
M
Linder
D
D
Schares
G
G
Schott
H H
HH
Schützle
B
B
Stirm
S
S
Stüber
W
W
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000606:Amino Sugars / Q000032:analysis; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005370:Filarioidea / Q000737:chemistry* / Q000276:immunology; D006023:Glycoproteins / Q000737:chemistry* / Q000276:immunology; D015801:Helminth Proteins / Q000737:chemistry* / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D008842:Microfilariae / Q000737:chemistry / Q000276:immunology; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology
D000596:Amino Acids; D000606:Amino Sugars; D006023:Glycoproteins; D015801:Helminth Proteins; D007106:Immune Sera; D010455:Peptides