Elimination of broad-host range plasmid vectors in Escherichia coli by curing agents.
A comparative study was made of the susceptibility of broad-host range vector plasmids belonging to Inc P1 and Q groups in Escherichia coli to various curing agents. Plumbagin and SDS eliminated RP4 (Inc P1 group) plasmid whereas pKT231 (Inc Q) and pRK2013 (having ColE1 replicon) were eliminated by hexamine ruthenium (III) chloride, alpha-santonin, coumermycin A1 and cis-dichloro diamine platinum (II). The curing activity of these agents was specific.
Bharathi
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Department of Microbiology, Osmania University, Hyderabad, India.
Polasa
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H
D049933:Aminocoumarins; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002945:Cisplatin / Q000494:pharmacology; D003374:Coumarins / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005822:Genetic Vectors; D009285:Naphthoquinones / Q000494:pharmacology; D010957:Plasmids / Q000187:drug effects*; D012428:Ruthenium / Q000494:pharmacology; D017975:Ruthenium Compounds; D012500:Santonin / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D049933:Aminocoumarins; D000900:Anti-Bacterial Agents; D003374:Coumarins; D009285:Naphthoquinones; D017975:Ruthenium Compounds; C031584:hexammineruthenium; D012500:Santonin; D012967:Sodium Dodecyl Sulfate; D012428:Ruthenium; C004628:coumermycin; D002945:Cisplatin; C014758:plumbagin