Effect of corticosteroid treatment on hemopoiesis in vivo and in vitro in a patient with Felty's syndrome.
We have studied the CFU-GM and BFU-E in vitro growth in a neutropenic and anemic patient with Felty's syndrome, either before or one and three months after steroid therapy when neutrophils and erythrocytes returned to normal. Both CFU-GM growth and CSA production were found to be low before therapy, and prednisone was shown to raise them to normal levels. The in vitro growth of BFU-E and the production of BPA by T lymphocytes of the patient were significantly lower than normal when studied before therapy. However, the T lymphocytes incubated in vitro with hydrocortisone regained their ability to stimulate the BFU-E growth. After prednisone therapy both BFU-E growth and BPA production by T lymphocytes returned to normal. Possible pathogenetic mechanisms of impaired granulo- and erythropoiesis in Felty's syndrome are discussed. The in vitro study with hydrocortisone can help to identify steroid-sensitive patients.
Moccia
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F
DIMI, Cattedra di Terapia Medica, University of Genoa, Italy.
Mazzarello
G P
GP
Morra
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L
D000305:Adrenal Cortex Hormones / Q000494:pharmacology* / Q000627:therapeutic use*; D015672:Erythroid Precursor Cells / Q000187:drug effects; D005258:Felty Syndrome / Q000188:drug therapy*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D066298:In Vitro Techniques; D008875:Middle Aged; D011241:Prednisone / Q000494:pharmacology
D000305:Adrenal Cortex Hormones; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D011241:Prednisone; D006854:Hydrocortisone