[The modification of a medium for culturing and isolating the causative agent of Lyme disease].
Possible use of three types of media (prepared on the basis of 199, RPMI and MEM, the rest of receipt being recommended for BSK-II) for cultivation of the agent of Lyme's disease is reviewed. The most satisfactory Borrelia burgdorferi growth has been noted on the medium with base 199. Using the same medium 5 spirochaete isolates have been obtained from 10 passages of the material from 41 ticks, Ixodes persulcatus Sch. and I. ricinus L. The results seem promising for further studies on the elaboration of Soviet medium with base 199 for the cultivation and isolation of the agent of Lyme's disease.
Gorelova
N B
NB
Shcherbakov
S V
SV
D001431:Bacteriological Techniques; D015748:Borrelia burgdorferi Group / Q000254:growth & development* / Q000302:isolation & purification; D003470:Culture Media
D003470:Culture Media
Modifikatsiia sredy dlia kul'tivirovaniia i izoliatsii vozbuditelia bolezni Laĭma.