[Changes in glutathione-S-transferase activity during induction of resistance of leukemia P 388 and Ehrlich ascitic tumor cells to doxorubicin].
We have studied by uridine short term test the level of resistance of murine leukemia cell lines P 388/Dx and ELD/Dx carcinoma cells with induced resistance to doxorubicin, P 388/Fp + Dx cells with induced resistance to combination of finoptOFF++ and doxorubicin in vivo. It was shown that the level of resistance was 6 fold for P 388/Dx cells, 4.5 fold for ELD/Dx cells and 2 fold for P 388/Fp + Dx cells. It was shown that the P 388/Dx cells and P 388/Fr + Dx cells had a 3.5 and 4.4 fold increase level of glutathione-S-transferase activity than P 388 cells. No increase in the activity of glutathione-S-transferase was detected in ELD/Dx cells. We conclude that increase of cellular glutathione-S-transferase activity is not associated with the development of resistance to doxorubicin.
Donenko
F V
FV
Kabieva
A O
AO
Borovkova
N B
NB
Moroz
L V
LV
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000201:enzymology; D004317:Doxorubicin / Q000627:therapeutic use*; D004351:Drug Resistance; D005982:Glutathione Transferase / Q000032:analysis*; D007941:Leukemia P388 / Q000188:drug therapy* / Q000201:enzymology; D014407:Tumor Cells, Cultured; D014700:Verapamil / Q000627:therapeutic use
D004317:Doxorubicin; D014700:Verapamil; D005982:Glutathione Transferase
Izmenenie aktivnosti glutation-S-transferaz pri induktsii rezistentnosti kletok leĭkoza P 388 i astsitnoĭ opukholi Erlikha k doksorubitsinu.