Collection and storage of capillary blood in glass fibre filters for glycated haemoglobin measurement by a microcolorimetric method.
A filter method for collection and storage of capillary blood spots for glycated haemoglobin (gHb) has been developed. Glass fibre filters (GFB) impregnated with 0.8 M boric acid were used to collect and store capillary blood. Haemoglobin from the dried blood spots was eluted into water and determined by Drabkin's method, while gHb in the eluates was determined by the microcolorimetric method. The intraassay coefficients of variation (CVs) were 4.5, 4.5 and 3.1% at 882, 1101 and 1704 pmol HMF/mg Hb, respectively. The corresponding inter-assay CVs were 8.6, 8.6 and 6.3%, respectively. A total of 63 paired capillary and venous blood samples were measured by both the direct and GFB method. The GFB method showed excellent correlation with the direct method (r = 0.948 and r = 0.994) after 7 and 14 days' storage at room temperature. The GFB method will enable prior collection and postage of blood samples by patients.
Ng
M L
ML
Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur.
Sazali
B S
BS
Khalid
B A
BA
D001800:Blood Specimen Collection / Q000379:methods*; D001888:Boric Acids; D002196:Capillaries; D003124:Colorimetry / Q000379:methods; D003920:Diabetes Mellitus / Q000097:blood*; D005374:Filtration / Q000295:instrumentation*; D005898:Glass; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods*
D001888:Boric Acids; D006442:Glycated Hemoglobin; C009753:fiberglass; C032688:boric acid