Micro-determination of L-gulono-gamma-lactone oxidase activity.
Highly sensitive assay method of L-gulono-gamma-lactone oxidase (GLO) was constructed. In this method, L-ascorbic acid formed in the enzymatic reaction was converted to its bis(dinitrophenyl)hydrazone derivative, and the amount of the latter was determined by high-performance liquid chromatography. Twenty picomoles of ascorbic acid was detected, which makes this method 25 times more sensitive than the previously used dipyridyl one. By the present method, a minute activity of GLO in liver microsomes prepared from rats of the Osteogenic Disorder Shionogi strain (ODS-od/od) could be measured.
Kito
M
M
Institute of Applied Biochemistry, Mitake, Gifu, Japan.
Ohishi
N
N
Yagi
K
K
D000818:Animals; D001205:Ascorbic Acid; D001848:Bone Diseases, Developmental / Q000201:enzymology / Q000235:genetics; D007700:Kinetics; D050544:L-Gulonolactone Oxidase; D008832:Microchemistry; D008862:Microsomes, Liver / Q000201:enzymology; D051381:Rats; D011922:Rats, Mutant Strains; D013401:Sugar Alcohol Dehydrogenases / Q000032:analysis* / Q000378:metabolism
D013401:Sugar Alcohol Dehydrogenases; D050544:L-Gulonolactone Oxidase; D001205:Ascorbic Acid