[Simple methods of enzymatic amplification of DNA for clinical practice].
The methods for enzymatic DNA amplification in vitro that allow to avoid the step of preliminary DNA extraction and purification are proposed. Lysates of blood cells in the solution or immobilized on the nylon membrane filters and dried blood spots on the filter paper blotters were used directly in amplification permitting one to solve the problems of adapting the method of polymerase chain reaction in clinical practice, for instance, in massive screening of genome mutations, viral infections etc.
Kuz'min
A I
AI
Khal'chitskiĭ
S E
SE
Skriabin
B V
BV
Kaboev
O K
OK
Shvarts
E I
EI
D000483:Alleles; D004247:DNA / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005784:Gene Amplification; D006801:Humans; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction
D015345:Oligonucleotide Probes; D004247:DNA; D004259:DNA-Directed DNA Polymerase
Prostye metody énzimaticheskoĭ amplifikatsii DNK dlia klinicheskoĭ praktiki.