title: Human placental protein methylase--I. Purification and characterization.
abstract: 1. Protein methylase I (S-adenosylmethionine[:]protein-arginine N-methyltransferase; EC 2.1.1.23) which methylates protein-bound arginine residues has been purified from human term placenta 400-fold with an approximate yield of 6%. 2. When histone was used as in vitro substrate, the methylation products were found to be NG-mono-, NG, NG-di- and NG, N'G-dimethylarginine. The enzyme was found to be sensitive toward Cu2+ with Ki value of 8 x 10(-5) M. The Km value for S-adenosyl-L-methionine was 5 x 10(-6) M. 3. When this partially purified protein methylase I was incubated with isolated human placental nuclei and S-adenosyl-L-[methyl-3H]methionine, the major endogenous [methyl-3H]-labeled proteins were protein species of 23, 38, 45 and 68 kDa, the 23 kDa species being the most predominant. 4. The endogenous enzyme activity during the pregnancy increased significantly, reaching more than 4 times the initial activity at the end of term.
authors.affiliation: Department of Biochemistry, School of Medicine, Wonkwang University, Iri, Chonbuk, Korea.
authors.forename: M K
authors.initials: MK
authors.lastname: Paik
authors.forename: K H
authors.initials: KH
authors.lastname: Lee
authors.forename: S S
authors.initials: SS
authors.lastname: Hson
authors.forename: I M
authors.initials: IM
authors.lastname: Park
authors.forename: J H
authors.initials: JH
authors.lastname: Hong
authors.forename: B D
authors.initials: BD
authors.lastname: Hwang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006657:Histones / Q000378:metabolism; D006801:Humans; D008745:Methylation; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000302:isolation & purification* / Q000378:metabolism; D011484:Protein-Arginine N-Methyltransferases / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D006657:Histones; D011257:Pregnancy Proteins; D003300:Copper; D011484:Protein-Arginine N-Methyltransferases