The uptake of bupivacaine in an in situ isolated perfused rabbit lung preparation.
A double indicator technique has been used in an in situ isolated perfused rabbit lung model to examine the first pass effect of the lung on systemic bupivacaine concentrations. Bupivacaine (0.5 mg/kg) was given in two consecutive boluses to six in situ isolated perfused New Zealand White rabbit lung preparations. The mean recovery (first bolus) of bupivacaine was 62.6% +/- 6.3 (S.E.M.), and 63.7% +/- 10.2 (second bolus), suggesting bupivacaine accumulation in the lung. The average mean transit time for bupivacaine was 280.5% +/- 24.1 and 264.8% +/- 36.7 longer than ICG (Indocyanine Green) following the first and second boluses respectively (P less than 0.01). There were no differences in the first pass effect of the lung between the first and second boluses of bupivacaine. The profiles of the bupivacaine concentrations suggest that uptake is followed by accumulation and later back diffusion. This has implications for conditions that decrease the uptake and therefore increase the risk of systemic toxicity.
Palazzo
M G
MG
Nuffield Department of Anaesthetics, Oxford, U.K.
Kalso
E
E
Argiras
E
E
Madgwick
R
R
Sear
J
J
D000818:Animals; D002045:Bupivacaine / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D007208:Indocyanine Green / Q000493:pharmacokinetics; D008168:Lung / Q000378:metabolism*; D010477:Perfusion; D011817:Rabbits
D007208:Indocyanine Green; D002045:Bupivacaine