title: A simple, rapid method for differential cell counts in porcine mammary secretions.
abstract: The use of the fluorescent dye acridine orange for making differential cell counts in mammary secretions from sows was investigated, and the variations in cell type during the lactation period were also studied. In untreated samples of mammary secretions polymorphonuclear leucocytes, mononuclear phagocytes, lymphocytes and epithelial cells could be identified with certainty, and the methodological error was small (1.80 to 2.22 per cent). The mammary secretions could be stored at 4 degrees C for six hours without any effect on the differential count. Washing the secretions decreased the percentage of polymorphonuclear leucocytes and increased the percentage of epithelial cells. The polymorphonuclear leucocytes predominated in colostrum (58.0 to 65.5 per cent) and epithelial cells predominated in milk (60 to 89 per cent). Polymorphonuclear leucocytes were the predominant phagocytes in all mammary secretions (7.6 to 65.5 per cent).
authors.affiliation: Department of Obstetrics and Gynaecology, College of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
authors.forename: U
authors.initials: U
authors.lastname: Magnusson
authors.forename: H
authors.initials: H
authors.lastname: Rodriguez-Martinez
authors.forename: S
authors.initials: S
authors.lastname: Einarsson
mesh_terms: D000165:Acridine Orange; D000818:Animals; D002452:Cell Count / Q000379:methods / Q000662:veterinary*; D003126:Colostrum / Q000166:cytology; D004847:Epithelial Cells; D005260:Female; D007958:Leukocyte Count / Q000379:methods / Q000662:veterinary; D008214:Lymphocytes / Q000648:ultrastructure; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism*; D008854:Microscopy, Electron / Q000662:veterinary; D008892:Milk / Q000166:cytology; D009504:Neutrophils / Q000648:ultrastructure; D010586:Phagocytes / Q000648:ultrastructure; D013552:Swine
chemical_list: D000165:Acridine Orange