Evaluation of an enzyme linked immunosorbent assay (ELISA) method for ABO and Lewis typing of body fluids in forensic samples.
An ELISA for the detection of the ABO group and secretor status of body fluids and stains other than blood is described, together with the validation procedures employed before its introduction into forensic casework. Criteria for the interpretation of results have been formulated for the method in use in this laboratory. The method was found to be reliable and to have a higher success rate than the haemagglutination techniques previously employed.
De Soyza
K
K
Metropolitan Police Forensic Science Laboratory, London, U.K.
D000017:ABO Blood-Group System; D000906:Antibodies; D000941:Antigens / Q000032:analysis*; D001826:Body Fluids / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005260:Female; D006386:Hemagglutination Tests; D006801:Humans; D007983:Lewis Blood Group Antigens; D008297:Male; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry; D012661:Semen / Q000737:chemistry; D014621:Vagina / Q000737:chemistry
D000017:ABO Blood-Group System; D000906:Antibodies; D000941:Antigens; D007983:Lewis Blood Group Antigens