title: Characterization and comparison of antimicrobial susceptibilities and outer membrane protein and plasmid DNA profiles of Pasteurella haemolytica and certain other members of the genus Pasteurella.
abstract: The outer membrane protein (OMP), plasmid, and antimicrobial resistance profiles of Pasteurella haemolytica serotypes 1 through 12, a bovine isolate of P multocida, a chicken isolate of P multocida, and an unidentified Pasteurella species of bovine origin were examined. Isolates of P haemolytica serotypes belonging to the same biotype possessed similar OMP profiles. Biotype A isolates contained 2 prominent OMP of 43 kilodaltons (kD) and 29 kD, whereas biotype-T serotypes contained 3 major OMP of 43, 36, and 25 kD. The major OMP profiles of the 2 P multocida isolates and the unidentified Pasteurella species were different from each other and from P haemolytica isolates. Plasmid DNA screening indicated both plasmid-containing and plasmid-free P haemolytica and P multocida isolates. Multiple drug resistance was found in pasteurellae isolates with and without plasmids. However, a relationship between drug resistance and plasmid isolation was found in 3 of 4 haemolytica serotype 1 field isolates, all of which contained a 2.51-megadalton plasmid and had multiple drug resistance for benzylpenicillin, ampicillin, streptomycin, and tetracycline.
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-5519.
authors.forename: S E
authors.initials: SE
authors.lastname: Rossmanith
authors.forename: G R
authors.initials: GR
authors.lastname: Wilt
authors.forename: G
authors.initials: G
authors.lastname: Wu
mesh_terms: D000668:Ampicillin Resistance; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D004269:DNA, Bacterial / Q000032:analysis*; D004352:Drug Resistance, Microbial; D016978:Mannheimia haemolytica / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics; D010325:Pasteurella / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics; D016979:Pasteurella multocida / Q000737:chemistry / Q000187:drug effects / Q000235:genetics; D010403:Penicillin Resistance; D010957:Plasmids / Q000187:drug effects; D013307:Streptomycin / Q000494:pharmacology; D013753:Tetracycline Resistance
chemical_list: D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D013307:Streptomycin