Rapid determination of abamectin in lettuce and cucumber by high-performance liquid chromatography.
A rapid, sensitive and reliable method is presented for the determination of trace amounts of abamectin in lettuce and cucumber. Abamectin consists of greater than or equal to 80% avermectin B1a and less than or equal to 20% avermectin Bb. Vegetables were extracted with ethyl acetate and the extract was purified by solid-phase extraction using Sep-Pak silica cartridges. The purified extracts were analysed by high-performance liquid chromatography with a 5 microm Zorbax ODS column and UV detection under isocratic conditions. The method yields recoveries for avermectin B1a and B1b of 76-109% in the 0.054-0.54 ng/kg range. The limit of detection of the method is 40 microgram/kg each of avermectin B1a and B1b in vegetables.
Vuik
J
J
TNO-CIVO Food Analysis Institute, The Netherlands.
D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis*; D007306:Insecticides / Q000032:analysis*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D014675:Vegetables / Q000737:chemistry*
D007306:Insecticides; C048324:abamectin; D007559:Ivermectin