title: Bovine herpesvirus 4 isolates: a comparison of three major glycoproteins.
abstract: Twenty-four Belgian field isolates of bovine herpesvirus 4 (BHV-4), together with four reference strains were compared by radio-immunoprecipitation and western blotting using a polyvalent antiserum and monoclonal antibodies raised against major glycoproteins. Most of these strains showed the same protein profile as the European reference strain Movar 33/63. For two strains the molecular weight of gp 6, p (gp 10/gp 17) and gp 10 were the same as those of the American reference strain DN 599. No relationship could be established between the protein profiles and origin of the isolates or with the restriction patterns. This study provides a view of the molecular weight variations of the major BHV-4 glycoproteins among field isolates.
authors.affiliation: Dept of Virology-Immunology, Faculty of Veterinary Medicine, University of Liège, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Dubuisson
authors.forename: M
authors.initials: M
authors.lastname: Bublot
authors.forename: G
authors.initials: G
authors.lastname: Wellemans
authors.forename: P P
authors.initials: PP
authors.lastname: Pastoret
authors.forename: E
authors.initials: E
authors.lastname: Thiry
mesh_terms: D000818:Animals; D001345:Autoradiography; D015153:Blotting, Western; D002417:Cattle; D005260:Female; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry; D006564:Herpesviridae / Q000737:chemistry*; D008297:Male; D008970:Molecular Weight; D015531:Radioimmunoprecipitation Assay; D014764:Viral Proteins / Q000032:analysis* / Q000737:chemistry
chemical_list: D006023:Glycoproteins; D014764:Viral Proteins