Improved immunoblotting for the detection and quantitative analysis of antibodies to human immunodeficiency virus.
Detection of antibodies to single HIV-1 proteins was performed by modified Western-blotting procedure. The use of [35S]-Streptavidin instead of Avidin-peroxidase conjugate greatly enhances the sensitivity of the method and enables useful quantitative analysis of results.
Portincasa
P
P
Institute of Microbiology, University of Parma, Medical School, Italy.
Conti
G
G
Gerloni
M
M
Chezzi
C
C
D001426:Bacterial Proteins; D015153:Blotting, Western / Q000379:methods*; D015483:HIV Antibodies / Q000032:analysis*; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D019809:Streptavidin; D013462:Sulfur Radioisotopes
D001426:Bacterial Proteins; D015483:HIV Antibodies; D013462:Sulfur Radioisotopes; D019809:Streptavidin