Molecular cloning and sequencing of Australian black bream Acanthopagrus butcheri and barramundi Lates calcarifer fish growth hormone cDNA using polymerase chain reaction.
The complementary DNA (cDNA) encoding the preprotein growth hormone (pre-GH) from two Australian marine fish species, namely Acanthopagrus butcheri and Lates calcarifer, have been isolated, cloned and sequenced. The sequences were amplified from reverse transcribed total RNA of whole brains using Polymerase Chain Reaction (PCR) and oligonucleotide primers corresponding to the 5' and 3' regions of Pagrus major. Use of PCR offers a rapid method of isolating fish GH cDNA sequences for commercial and taxonomic applications. Sequence comparison indicates a high degree of conservation for GH cDNAs within the family Sparidae.
Knibb
W
W
Department of Biochemistry, University of Adelaide, Australia.
Robins
A
A
Crocker
L
L
Rizzon
J
J
Heyward
A
A
Wells
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D005399:Fishes / Q000235:genetics*; D013006:Growth Hormone / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D011498:Protein Precursors; D013006:Growth Hormone; D004247:DNA