title: Imaging cytometry by multiparameter fluorescence.
abstract: A system is described for performing multicolor fluorescence image cytometry of cell preparations. After the setting up stage, the operation is automatic: the microscope fields are found and focused; then images are acquired for each fluorophore, corrected and analyzed, without any operator interaction. Human peripheral blood lymphocytes on microscope slides were used as a test system. In these experiments, three fluorescent antibodies were used to identify lymphocyte sub-populations, and a DNA content probe was used to identify all nucleated cells. The cell subset percentages determined by image cytometry were comparable to percentages obtained when cells from the same preparation were analyzed by flow cytometry. Multicolor fluorescence imaging cytometry can potentially be extended to the analysis of cells in smears, fine needle biopsies, imprints, and tissue sections.
authors.affiliation: Department of Laboratory Medicine, Allegheny Singer Research Institute, Pittsburgh, PA 15212.
authors.forename: W
authors.initials: W
authors.lastname: Galbraith
authors.forename: M C
authors.initials: MC
authors.lastname: Wagner
authors.forename: J
authors.initials: J
authors.lastname: Chao
authors.forename: M
authors.initials: M
authors.lastname: Abaza
authors.forename: L A
authors.initials: LA
authors.lastname: Ernst
authors.forename: M A
authors.initials: MA
authors.lastname: Nederlof
authors.forename: R J
authors.initials: RJ
authors.lastname: Hartsock
authors.forename: D L
authors.initials: DL
authors.lastname: Taylor
authors.forename: A S
authors.initials: AS
authors.lastname: Waggoner
mesh_terms: D000465:Algorithms; D001185:Artificial Intelligence; D003204:Computers, Mainframe; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods*; D016131:Lymphocyte Subsets / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D012984:Software
chemical_list: D005456:Fluorescent Dyes