Conglutinin exhibits a complement-dependent enhancement of the respiratory burst of phagocytes stimulated by E. coli.
Conglutinin is a mammalian C-type lectin which shows anti-bacterial activity when tested in vivo and in vitro. This study concerns the effect of conglutinin on the respiratory burst of murine spleen cells, using a chemiluminescence assay for measurement of generated reactive oxygen metabolites. Conglutinin enhances, in a dose-dependent manner, the respiratory burst of spleen cells stimulated with serum-opsonized Escherichia coli. The enhancement was only demonstrable in the presence of a functional complement system. The conglutinin-mediated enhancement of the respiratory burst was inhibited in the presence of a N-acetyl-D-glucosamine, D-mannose and N-acetyl-D-mannosamine, monosaccharides reported to inhibit conglutinin-binding to zymosan and the complement factor iC3b. On the other hand, N-acetyl-D-galactosamine was non-inhibitory.
Friis
P
P
Department of Medical Microbiology, Odense University, Denmark.
Svehag
S E
SE
Andersen
O
O
Gahrn-Hansen
B
B
Leslie
R G
RG
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D002417:Cattle; D037222:Collectins; D003165:Complement System Proteins / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D004926:Escherichia coli / Q000276:immunology*; D008163:Luminescent Measurements; D051379:Mice; D009005:Monosaccharides / Q000494:pharmacology; D010586:Phagocytes / Q000276:immunology*; D012712:Serum Globulins / Q000037:antagonists & inhibitors / Q000276:immunology*; D013154:Spleen / Q000276:immunology
D000942:Antigens, Bacterial; D037222:Collectins; D009005:Monosaccharides; D012712:Serum Globulins; C024014:conglutinin; D003165:Complement System Proteins