15-Hydroxyprostaglandin dehydrogenase activity in the lower genitourinary tract. A preliminary report.
In the pathway of prostaglandin inactivation, 15-hydroxyprostaglandin dehydrogenase (15-OHPGDH) has been proven to be the catalyst of the primary catabolic step in the oxidation of the 15-hydroxyl group into a 15-keto moiety in most derivatives of prostaglandins. In this study, we analysed 15-OHPGDH activity in the lower genitourinary tract. The specimens were obtained with permission from patients who underwent surgery. These specimens were then sent for quantitative determination using a tritium release assay specific for 15-OHPGDH. The highest enzyme activity was found in the ureter with a mean +/- SE (pmol/min/mg) of 94.99 +/- 51.00; the lowest activity was in the vas deferens, with a value of 0.18 +/- 0.21. This preliminary study indicated that the activity of 15-OHPGDH in the lower genitourinary tract was significant and that prostaglandin levels in the genitourinary tract may be regulated by this enzyme.
Chang
C C
CC
Department of Urology, National Cheng Kung University Medical College, Tainan, Taiwan, China.
Lin
S N
SN
Chen
F S
FS
Chang
W C
WC
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011467:Prostate / Q000378:metabolism*; D014513:Ureter / Q000201:enzymology*; D014521:Urethra / Q000201:enzymology*; D001743:Urinary Bladder / Q000201:enzymology*
D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase