Avoiding gas bubble formation during freeze substitution.
Gas bubbles frequently are formed during freeze substitution, especially when tissues are warmed to room temperature. The problem arises largely from the extreme solubility of CO2 in the freeze substitution solvent. Gas bubbles may be minimized by briefly transferring the tissue to freshly chilled solvent before warming to room temperature.
Fisher
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Department of Botany, Washington State University, Pullman 99164-4238.
D002245:Carbon Dioxide; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005629:Frozen Sections; D005740:Gases; D013696:Temperature
D005740:Gases; D002245:Carbon Dioxide