Mutans streptococci and non-mutans streptococci acidogenic at low pH, and in vitro acidogenic potential of dental plaque in two different areas of the human dentition.
Samples of human dental plaque were obtained from sound tooth surfaces in the lower anterior and upper posterior areas of each of 11 subjects with various degrees of caries experience. Both types of plaque were compared for: (1) their pH-lowering potential [pH at 10 and 60 min after sugar addition and the pH drop between 0 and 10 min (delta pH)] with an in vitro method involving dispersed plaque suspensions and excess glucose supply; (2) the proportions of mutans streptococci; and (3) the distribution of the predominant non-mutans streptococci according to their final pH in glucose broth. Compared with plaque from the lower anterior area, plaque from the upper posterior area exhibited a significantly higher pH-lowering potential, i.e., a lower pH at 10 and 60 min and a greater delta pH and significantly higher levels of mutans streptococci. The final pH values for the non-mutans streptococci exhibited a wide range from about 4.4 to over 5.0. The proportions of such organisms designated as capable of acidogenesis at low pH (final pH less than 4.6), whether expressed as a percentage of the total non-mutans streptococci or of the total plaque flora, were significantly increased in plaque from the upper posterior area. The proportions of non-mutans streptococci capable of acidogenesis at low pH in plaque from the upper posterior area were also significantly increased, with decreasing pH values at 10 and 60 min.(ABSTRACT TRUNCATED AT 250 WORDS)
Van Houte
J
J
Department of Oral Microbiology, Forsyth Dental Center, Boston, Massachusetts 02115.
Sansone
C
C
Joshipura
K
K
Kent
R
R
D000143:Acids; D000293:Adolescent; D000328:Adult; D015169:Colony Count, Microbial; D003773:Dental Plaque / Q000382:microbiology* / Q000473:pathology / Q000503:physiopathology; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008875:Middle Aged; D013291:Streptococcus / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D013295:Streptococcus mutans / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D016986:Streptococcus sobrinus / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology
D000143:Acids