[Introduction of aminoalkyl groups into prepared synthetic oligodeoxyribonucleotides by transamination of cytosine residues].
The bisulphite-catalysed transamination of cytosine residues by means of ethylenediamine generally used for the natural nucleic acids modification has been extended on relatively short synthetic oligonucleotides. One of the aminoalkyloligonucleotides thus obtained has been used for preparing a biotinylated hybridisation probe.
Karpyshev
N N
NN
Bondarenko
T Iu
TIu
Vtorushina
I A
IA
Kipriianov
S M
SM
D000478:Alkylation; D000588:Amines / Q000737:chemistry*; D001483:Base Sequence; D001710:Biotin / Q000378:metabolism; D003596:Cytosine / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*
D000588:Amines; D009838:Oligodeoxyribonucleotides; D001710:Biotin; D003596:Cytosine
Vvedenie aminoalkil'nykh grupp v gotovye sinteticheskie oligodezoksiribonukleotidy putem pereaminirovaniia ostatkov tsitozina.