Re-evaluation of rate constants involved in the action of the initiation and elongation factors eIF-2B and EF-Ts.
Rate constants calculated previously by the author (Biochem. Int. 22, 523-533:1990) for the reactions catalysed by eIF-2B (GEF) in which free GDP exchanges with GDP bound to eIF-2 have been re-evaluated using the computational procedures developed by Chau et al. (J. Biol. Chem. 256, 5591-5596:1981) for the analogous reactions catalysed by EF-Ts. Modification of the equations used by Chau et al. emphasises the interrelationships of the rate constants for the binding of GDP and of EF-Ts (eIF-2B) to the ternary complex EF-Ts.EF-Tu.GDP (eIF-2B.eIF-2.GDP). The modification leads to some revision of the previously published values for the rate constants involved in the action of EF-Ts as put forward by Chau et al. as well as for those involved in the action of eIF-2B.
Manchester
K L
KL
Department of Biochemistry, University of the Witwatersrand, Johannesburg, South Africa.
D020662:Guanine Nucleotide Exchange Factors; D006153:Guanosine Diphosphate / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D010445:Peptide Elongation Factors / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*
D020662:Guanine Nucleotide Exchange Factors; D010445:Peptide Elongation Factors; D011506:Proteins; C023512:elongation factor Ts; D006153:Guanosine Diphosphate