title: Crystals of isoenzyme 3-3 of rat liver glutathione S-transferase with and without inhibitor.
abstract: The isoenzyme 3-3 of rat liver glutathione S-transferase (GST 3-3) isolated from a baculovirus expression system has been crystallized with and without inhibitor. The crystals grown in the absence of an inhibitor belong to space group P2(1) with cell dimensions a = 119.7, b = 96.2, c = 136.7 A and beta = 103.3 degrees, and diffract to 3 A resolution. The crystals grown in the presence of an inhibitor belong to space group C2 with cell dimensions a = 88.3, b = 69.7, c = 81.4 A and beta = 105.3 degrees, and diffract to at least 2.5 A resolution. The inhibitor used is either methylmercury chloride or ethylmercury chloride; both are weak inhibitors.
authors.affiliation: Department of Crystallography, University of Pittsburgh, PA 15260.
authors.forename: J H
authors.initials: JH
authors.lastname: Fu
authors.forename: J
authors.initials: J
authors.lastname: Rose
authors.forename: Y J
authors.initials: YJ
authors.lastname: Chung
authors.forename: M F
authors.initials: MF
authors.lastname: Tam
authors.forename: B C
authors.initials: BC
authors.lastname: Wang
mesh_terms: D000818:Animals; D003461:Crystallography; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000737:chemistry*; D007527:Isoenzymes / Q000737:chemistry*; D008099:Liver / Q000201:enzymology*; D015394:Molecular Structure; D051381:Rats; D014961:X-Ray Diffraction
chemical_list: D007527:Isoenzymes; D005982:Glutathione Transferase