Activity of some proteinases and glycosidases in human leukemic lymphoid cells at various stages of differentiation.
Activities of some glycosidases and proteinases in human leukemic lymphoid cells at various stages of differentiation have been compared. It was found that cells with different immunological phenotypes gave different enzymic spectra. Glycosidases and proteinases in lymphoid cell precursors had higher activity level than the enzymes in mature T- and B- cells. In cells of B- lineage, all activities were lower than in common precursor of lymphoid cells. In T-cells at the earlier stages of thymic differentiation, activities of all proteinases and most of glycosidases were higher than in common precursor cells whereas in mature T-helpers and T-suppressors the activities were markedly lower. Most of hydrolases in mature T-cells were twice more active than the enzymes in mature B-cells. The opposite-directional changes in activities of some hydrolases at the earlier stages of differentiation of lymphoid cells along B- or T- cells pathways are suggested.
Lokshina
L A
LA
Institute of Biological and Medical Chemistry, USSR Academy of Medical Sciences, Moscow.
Preobrazhenskaya
M E
ME
Samojlova
R S
RS
Bylinkina
V S
VS
Ushakova
N A
NA
Gureeva
T A
TA
Golubeva
N V
NV
D000943:Antigens, Differentiation; D001402:B-Lymphocytes / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D002454:Cell Differentiation; D010450:Endopeptidases / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006801:Humans; D007945:Leukemia, Lymphoid / Q000201:enzymology / Q000473:pathology; D008214:Lymphocytes / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000201:enzymology / Q000473:pathology; D013601:T-Lymphocytes / Q000201:enzymology / Q000276:immunology / Q000473:pathology
D000943:Antigens, Differentiation; D006026:Glycoside Hydrolases; D010450:Endopeptidases