title: Refolding kinetics of pig muscle and yeast 3-phosphoglycerate kinases and of their proteolytic fragments.
abstract: The time course of refolding of both pig muscle and yeast 3-phosphoglycerate kinase (molecular masses about 47 kDa), as well as their proteolytic C-terminal fragments (30 and 33 kDa, respectively) has been investigated. Very similar refolding kinetics (with half-time between 80-120 s, at 20 degrees C) were observed by fluorescence and ultraviolet absorbance spectroscopy, as well as by activity measurements, for the intact enzyme from both sources. This time course appears not to depend on the time the protein spends in the unfolded state, i.e. it is certainly not controlled by proline isomerization. Furthermore, after removal of a large N-terminal part (molecular mass of about 18 kDa for pig muscle enzyme or 13 kDa for yeast enzyme) of the molecule by proteolysis, refolding of the remaining C-terminal fragment of both proteins follows kinetics virtually indistinguishable from those of the intact protein molecule.
authors.affiliation: Institute of Protein Research, USSR Academy of Sciences, Pushchino.
authors.forename: G V
authors.initials: GV
authors.lastname: Semisotnov
authors.forename: M
authors.initials: M
authors.lastname: Vas
authors.forename: V V
authors.initials: VV
authors.lastname: Chemeris
authors.forename: N J
authors.initials: NJ
authors.lastname: Kashparova
authors.forename: N V
authors.initials: NV
authors.lastname: Kotova
authors.forename: O I
authors.initials: OI
authors.lastname: Razgulyaev
authors.forename: M A
authors.initials: MA
authors.lastname: Sinev
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002151:Calorimetry; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010735:Phosphoglycerate Kinase / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D011489:Protein Denaturation; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D013997:Time Factors
chemical_list: D010446:Peptide Fragments; D010735:Phosphoglycerate Kinase