title: Different porphobilinogen-deaminase forms in wild and mutant strains of Saccharomyces cerevisiae. A possible correlation with its segregants behaviour.
abstract: 1. Different porphobilinogen-deaminase (PBG-D) enzyme forms were found for D 27 and D 27/C6 (HEM R+) strains of Saccharomyces cerevisiae. 2. PBG-D was partially purified and chromatographed on Sephadex G-100 in either the presence or absence of a protease inhibitor. For D 27 only one active peak was observed while for D 27/C6 strain two active peaks were found. 3. A correlation between this differential behaviour and the presence of HEM R+ gene was looked for employing two segregants of one tetrad from D 27 and D 27/C6 mating.
authors.affiliation: Centro de Investigaciones sobre Porfirinas y Porfirias-CIPYP (CONICET, F.C.E. y N., UBA), Ciudad Universitaria, Buenos Aires, Argentine.
authors.forename: S R
authors.initials: SR
authors.lastname: Correa Garcia
authors.forename: M V
authors.initials: MV
authors.lastname: Rossetti
authors.forename: A M
authors.initials: AM
authors.lastname: Batlle
mesh_terms: D000642:Ammonia-Lyases / Q000235:genetics* / Q000378:metabolism; D002850:Chromatography, Gel; D007527:Isoenzymes / Q000235:genetics; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics
chemical_list: D007527:Isoenzymes; D000642:Ammonia-Lyases; C100132:porphobilinogenase