High affinity interleukin-6 binding sites in bovine hypothalamus.
The cytokine interleukin-6 (IL-6) has been proposed to interact with the hypothalamic-pituitary axis. The present data provide the first reported evidence of high affinity binding sites for [125I]IL-6 in brain tissue, specifically bovine hypothalamic membranes. Binding was saturable and represented a single site, with a Kd of 316 +/- 49 pM and receptor density of 15.8 +/- 3.2 fmol/mg protein. Other cytokines tested did not interact with this site, but a neutralizing monoclonal anti-IL-6 antibody blocked specific binding. These findings support the proposed involvement of IL-6 in communication between neural and immune systems.
Cornfield
L J
LJ
Ciba-Geigy Corporation, Research Department, Summit, NJ 07901.
Sills
M A
MA
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D015850:Interleukin-6 / Q000378:metabolism*; D007457:Iodine Radioisotopes; D011971:Receptors, Immunologic / Q000502:physiology*; D019947:Receptors, Interleukin-6
D000911:Antibodies, Monoclonal; D015850:Interleukin-6; D007457:Iodine Radioisotopes; D011971:Receptors, Immunologic; D019947:Receptors, Interleukin-6