Proteins of mammalian mitochondrial ribosomes.
The bovine mitochondrial system is being developed as a model system for studies on mammalian mitochondrial ribosomes. Information is emerging on the structural organization and RNA binding properties of proteins in these mitochondrial ribosomes. Unexpectedly, these ribosomes appear to interact directly with GTP, via a high affinity binding site on the small subunit. Despite major differences in their RNA content and physical properties, mammalian mitochondrial and cytoplasmic ribosomes contain about the same number of proteins. The proteins in each kind of ribosome have a similar size distribution, and both sets are entirely coded by nuclear genes, raising the possibility that these different ribosomes may contain the same set of proteins. Comparison of bovine mitochondrial and cytoplasmic r-proteins by co-electrophoresis in two-dimensional gels reveals that most of the cytoplasmic ribosomal proteins are more basic than the mitochondrial ribosomal proteins, and that none are co-migratory with mitochondrial ribosomal proteins, suggesting that the proteins in the two ribosomes are different. To exclude the possibility that the electrophoretic differences result only from post-translational modification of otherwise identical proteins, antibodies against several proteins from the large subunit of bovine mitochondrial ribosomes were tested against cytoplasmic ribosomes by solid phase radioimmunoassay and against cytoplasmic ribosomal proteins on Western blots. The lack of cross-reaction of these antibodies with cytoplasmic r-proteins suggests that mitochondrial ribosomal proteins have different primary structures and thus are most likely encoded by a separate set of nuclear genes.
Pietromonaco
S F
SF
Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville 32610.
Denslow
N D
ND
O'Brien
T W
TW
D000818:Animals; D000918:Antibody Specificity; D005075:Biological Evolution; D002417:Cattle; D003429:Cross Reactions; D003593:Cytoplasm / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D008930:Mitochondria, Liver / Q000737:chemistry*; D008954:Models, Biological; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D012270:Ribosomes / Q000737:chemistry*
D012269:Ribosomal Proteins