Quantification of apolipoprotein D in human urine by zone immunoelectrophoresis assay: a methodological and clinical study.
A zone immunoelectrophoresis assay (ZIA) has been developed for the quantification of apolipoprotein D (apo D) in unconcentrated native human urine. A standard curve, linear between 1 and 8 mg apo D/l was obtained with ZIA. The relative coefficients of variation for this method were 5-9% (n = 15 x 6) with a mean +/- SD of 7 +/- 1.4% and below 11% (n = 6 x 15) for within-run and between-run reproducibility, respectively. Equal amounts of apo D in unconcentrated and diluted urines, in serum and of the purified protein produced the same zone migration distances indicating parallelism between the immunologic reactions of apo D in different sample matrixes. Storage experiments with normal urines demonstrated good stability of apo D in both acidic and alkalinized urine over at least 2 days at +5 degrees C and during several days at -20 degrees C to -40 degrees C. Using ZIA, urine samples from 50 normal healthy men aged 23-65 years were analyzed for apo D. Mean and SD were: 2.8 +/- 2.1 mg/l, 2.6 +/- 1.8 micrograms/min and 0.24 +/- 0.13 mg/mmol for concentration, rate of excretion and mass/creatinine concentration, respectively.
Holmquist
L
L
Division of Medical Chemistry, National Institute of Occupational Health, Solna, Sweden.
Vesterberg
O
O
D000328:Adult; D000368:Aged; D001053:Apolipoproteins / Q000097:blood / Q000652:urine*; D053399:Apolipoproteins D; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D011233:Precipitin Tests
D001053:Apolipoproteins; D053399:Apolipoproteins D