Altered expression of the two naturally occurring human insulin receptor variants in isolated adipocytes of non-insulin-dependent diabetes mellitus patients.
The human insulin receptor gene is expressed in two variant isoforms which differ by the absence (HIR-A) or presence (HIR-B) of 12 amino acids in the COOH-terminus of the extracellular alpha-subunit as a consequence of alternative splicing of exon 11. Expression of the two variant isoforms is regulated in a tissue-specific manner. In this study, we have measured the levels of the two receptor variants in isolated adipocytes from 10 non-insulin-dependent diabetes mellitus (NIDDM) and 11 normal subjects using an immunological assay, based on the ability of a human anti-receptor autoantibody to discriminate between HIR-A and HIR-B. Results indicate that levels of HIR-B variant are increased in NIDDM patients.
Sesti
G
G
Dipartimento di Medicina Interna, Università di Roma Tor Vergata, Italy.
Marini
M A
MA
Tullio
A N
AN
Montemurro
A
A
Borboni
P
P
Fusco
A
A
Accili
D
D
Lauro
R
R
D016475:3T3 Cells; D000273:Adipose Tissue / Q000502:physiology / Q000503:physiopathology*; D000595:Amino Acid Sequence; D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D014644:Genetic Variation; D006801:Humans; D007074:Immunoglobulin G; D007328:Insulin / Q000378:metabolism; D007700:Kinetics; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008875:Middle Aged; D011972:Receptor, Insulin / Q000235:genetics* / Q000378:metabolism; D012016:Reference Values; D014162:Transfection
D007074:Immunoglobulin G; D007328:Insulin; D046911:Macromolecular Substances; D011972:Receptor, Insulin