title: Gap-junctional permeability in early and cleavage-arrested ascidian embryos.
abstract: Using the whole-cell voltage clamp technique, we have studied junctional conductance (Gj), and Lucifer Yellow (LY) coupling in 2-cell and 32-cell ascidian embryos. Gj ranges from 17.5 to 35.3 nS in the 2-cell embryo where there is no passage of LY, and from 3.5 to 12.2 nS in the later embryo where LY dye spread is extensive. In both cases, Gj is independent of the transjunctional potential (Vj). Manually apposed 2-cell or 32-cell embryos established a junctional conductance of up to 10 nS within 30 min of contact. Furthermore, since we did not observe any significant number of cytoplasmic bridges at the EM and Gj is sensitive to octanol, it is probable that blastomeres in the 2-cell and 32-cell embryos are in communication by gap junctions. In order to compare Gj in the two stages and to circumvent problems of cell size, movement and spatial location, we used cytochalasin B to arrest cleavage. Gj in cleavage-arrested 2-cell embryos ranged from 25.0 to 38.0 nS and remained constant over a period of 2.5 h. LY injected into a blastomere of these arrested embryos did not spread to the neighbour cell until they attained the developmental age of a 32- to 64-cell control embryo. Our experiments indicate a change in selectivity of gap junctions at the 32-cell stage that is not reflected by a macroscopic change in ionic permeability.
authors.affiliation: Stazione Zoologica, Naples, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Dale
authors.forename: L
authors.initials: L
authors.lastname: Santella
authors.forename: E
authors.initials: E
authors.lastname: Tosti
mesh_terms: D000818:Animals; D001757:Blastomeres / Q000187:drug effects / Q000648:ultrastructure; D002970:Cleavage Stage, Ovum / Q000502:physiology* / Q000648:ultrastructure; D003571:Cytochalasin B / Q000494:pharmacology; D004553:Electric Conductivity / Q000502:physiology; D007365:Intercellular Junctions / Q000502:physiology*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D014561:Urochordata / Q000196:embryology / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D003571:Cytochalasin B