Comparison of the bindin proteins of Strongylocentrotus franciscanus, S. purpuratus, and Lytechinus variegatus: sequences involved in the species specificity of fertilization.
Bindin is the sea urchin sperm acrosomal protein that is responsible for the species-specific adhesion of the sperm to the egg. Two new bindin cDNA sequences that contain the entire open reading frame for the binding precursor are reported: one for Strongylocentrotus franciscanus and one for Lytechinus variegatus. Both contain inverted repetitive sequences in their 3' untranslated regions, and the S. franciscanus cDNA contains an inverted repetitive sequence match between the 5' untranslated region and the coding region. The middle third of the mature bindin sequence is highly conserved in all three species, and the flanking sequences share short repeated sequences that vary in number between the species. Cross-fertilization data are reported for the species S. purpuratus, S. franciscanus, L. variegatus, and L. pictus. A barrier to cross-fertilization exists between the sympatric Strongylocentrotus species, but there is no barrier between the allopatric Lytechinus species.
Minor
J E
JE
Division of Biology, California Institute of Technology, Pasadena 91125.
Fromson
D R
DR
Britten
R J
RJ
Davidson
E H
EH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D004247:DNA; D005260:Female; D005306:Fertilization / Q000235:genetics*; D014644:Genetic Variation; D006023:Glycoproteins / Q000235:genetics* / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D012091:Repetitive Sequences, Nucleic Acid; D012617:Sea Urchins / Q000145:classification / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D006023:Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C035027:egg surface sperm receptor; D004247:DNA