Assay of nicergoline and three metabolites in human plasma and urine by high-performance liquid chromatography-atmospheric pressure ionization mass spectrometry.
A method for the simultaneous determination of nicergoline and three of its metabolites in human plasma and urine has been developed using high-performance liquid chromatography-atmospheric pressure ionization mass spectrometry. Nicergoline and its metabolites were extracted from the plasma and urine samples with chloroform and separated on a reversed-phase ODS column. The eluents were led to the atmospheric pressure ionization interface and then analysed in the selected-ion monitoring mode. The detection limits of nicergoline and three of its metabolites were ca. 2 ng/ml in plasma and ca. 10 ng/ml in urine, at a signal-to-noise ratio of 4.
Banno
K
K
Analytical Chemistry Research Laboratory, Tanabe Seiyaku Co., Ltd., 16-89, Osaka, Japan.
Horimoto
S
S
Mabuchi
M
M
D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007477:Ions; D013058:Mass Spectrometry; D009530:Nicergoline / Q000097:blood / Q000378:metabolism* / Q000652:urine; D012016:Reference Values
D007477:Ions; D009530:Nicergoline