Cloning and expression in Escherichia coli of a recA homologue from Mycobacterium tuberculosis.
A 3.8 kb PstI fragment of Mycobacterium tuberculosis was cloned in a recA-deleted Escherichia coli by selecting transformants with increased EMS resistance. The cloned fragment restored homologous recombination in Hfr crosses and conferred resistance to long wave (302 nm) but not short wave (254 nm) UV light. E. coli containing the 3.8 kb PstI fragment produced a 38-40 kDa protein which cross-reacted with antibodies raised against the E. coli RecA protein. The cloned DNA thus probably encodes a RecA homologue.
Nair
S
S
Department of Medical Microbiology, University of Cape Town, Medical School, Observatory, South Africa.
Steyn
L M
LM
D000907:Antibodies, Bacterial / Q000276:immunology; D000918:Antibody Specificity; D003227:Conjugation, Genetic; D003429:Cross Reactions; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology / Q000528:radiation effects; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000276:immunology; D011938:Rec A Recombinases / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D011995:Recombination, Genetic; D014466:Ultraviolet Rays
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D011994:Recombinant Proteins; D011938:Rec A Recombinases