A sensitive reverse passive haemagglutination immunoassay for hepatic caeruloplasmin.
A reverse passive haemagglutination assay for the measurement of caeruloplasmin in homogenates and organelle fractions prepared from needle biopsies of human liver tissue is described. Use of serial dilutions over a narrow range of concentrations permits accurate quantitation of caeruloplasmin down to 10 ng/ml, sufficient to detect the protein in needle biopsies with as little as 5 mg wet weight of tissue. Applied to the measurement of caeruloplasmin in human sera, the assay gives values which correlate well with those obtained by the standard radial immunodiffusion technique.
Davis
W
W
Physiological Laboratory, Cambridge, U.K.
Seymour
C A
CA
D001707:Biopsy, Needle; D002570:Ceruloplasmin / Q000032:analysis*; D006386:Hemagglutination Tests; D006801:Humans; D007118:Immunoassay; D008099:Liver / Q000737:chemistry* / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000737:chemistry
D002570:Ceruloplasmin