Concentration of lipid peroxide in serum lipoproteins of insulin-dependent diabetic children.
Lipids, apolipoproteins and lipid peroxide were measured in the sera of 29 children with insulin-dependent diabetes mellitus. Ten non-diabetic children served as controls. High-density lipoprotein (HDL) was separated by heparin-MnCl2 precipitation. Lipid peroxides in HDL and non-HDL fractions were estimated by fluorimetric measurement of thiobarbituric acid-reactive substances. The patients were normolipidemic, and their HDL-cholesterol was increased. Apo A1 level in the patients was similar to that in the controls, while levels of apo A2 and apo B were decreased in the patients. Concentrations of lipid peroxides in the whole serum and non-HDL were unaltered, while that in the HDL was higher in the patients than in the controls. Hemoglobin AIc in the patients correlated with the triglyceride and urinary excretion rate of N-acetylglucosaminidase (NAG). The NAG correlated with the triglycerides. The triglycerides correlated with the atherogenic index, apo B and total cholesterol. The lipid peroxides in the non HDL correlated with the triglyceride, atherogenic index, and NAG. That in the HDL correlated with the HDL-cholesterol, apo A1 and endogenous creatinine clearance, and inversely with the atherogenic index and apo B. Lipid peroxides in HDL and non-HDL appeared to play different physiological roles from each other, and they have provided evidence suggesting that diabetic microvascular injury is mediated by reactive oxygen species.
Asayama
K
K
Department of Pediatrics, Yamanashi Medical College, Japan.
Miyao
A
A
Dobashi
K
K
Amemiya
S
S
Ishihara
T
T
Kato
K
K
D000118:Acetylglucosaminidase / Q000652:urine; D001054:Apolipoproteins A / Q000737:chemistry*; D001055:Apolipoproteins B / Q000097:blood* / Q000737:chemistry; D002648:Child; D002784:Cholesterol / Q000097:blood* / Q000737:chemistry; D008076:Cholesterol, HDL / Q000097:blood / Q000737:chemistry; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D005260:Female; D005470:Fluorometry / Q000379:methods; D006441:Hemoglobin A / Q000032:analysis; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology*; D008054:Lipid Peroxides / Q000097:blood* / Q000737:chemistry; D008075:Lipoproteins, HDL / Q000097:blood* / Q000737:chemistry; D008297:Male; D014280:Triglycerides / Q000097:blood
D001054:Apolipoproteins A; D001055:Apolipoproteins B; D008076:Cholesterol, HDL; D008054:Lipid Peroxides; D008075:Lipoproteins, HDL; D014280:Triglycerides; D006441:Hemoglobin A; D002784:Cholesterol; D000118:Acetylglucosaminidase