Ca(2+)-responsive extensible monolayer membrane of calmodulin-albumin conjugate.
A Ca(2+)-responsive monolayer protein membrane was prepared by developing calmodulin and bovine serum albumin at the air-water interface and by conjugating them with a bifunctional agent. In the case of the BSA monolayer, complex formation with Mg2+ generated a larger change in surface pressure than that with Ca2+. On the other hand, a drastic change in surface pressure was observed for the conjugated thin membrane associated with Ca2+ than that associated with Mg2+. Due to a drastic change in the conformation of calmodulin, the conjugated protein film changes its morphology (STM image), depending on Ca2+ concentration: the extended structure in the presence of Ca2+ transforms to a shrinked structure in the absence of Ca2+. The largest surface pressure change was detected when calmodulin was mixed with an equimolar amount of bovine serum albumin.
Miwa
T
T
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Kobatake
E
E
Ikariyama
Y
Y
Aizawa
M
M
D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D004337:Drug Carriers / Q000138:chemical synthesis; D007700:Kinetics; D008566:Membranes / Q000737:chemistry; D016252:Microscopy, Scanning Tunneling; D010281:Parathyroid Hormone / Q000008:administration & dosage; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013997:Time Factors
D002147:Calmodulin; D004337:Drug Carriers; D010281:Parathyroid Hormone; D012710:Serum Albumin, Bovine; D002118:Calcium