title: Characterization of lipoprotein secreted by cultured eel hepatocytes and its comparison with serum lipoproteins.
abstract: The lipoprotein secreted by cultured eel hepatocytes was fractionated by density gradient ultracentrifugation and compared with eel serum lipoproteins. Eel hepatocytes were cultured for 7 to 10 days as a monolayer in Williams' medium E containing 5% fetal bovine serum and 0.16 microM insulin on a dish precoated with fibronectin of horse serum. The only lipoprotein secreted by eel hepatocytes was a very-low-density lipoprotein like one which consisted of 69% triglyceride, 15% phospholipid, 4% cholesterol, and 12% protein. On the other hand, very-low-density lipoprotein and high density lipoprotein were found in eel serum, in which high density lipoprotein was a main lipoprotein. The secreted lipoprotein contained apo B and apo A as the main protein components. Furthermore, the lipoprotein contained proapo A-I in addition to apo A-I, which was proved by comparing the amino acid composition of both proteins. In our discussion, we noted that the lipoprotein secreted by eel hepatocytes was a good material for the study of high-density lipoprotein formation.
authors.affiliation: Laboratory of Food Chemistry, Faculty of Fisheries, Kagoshima University, Japan.
authors.forename: F G
authors.initials: FG
authors.lastname: Yu
authors.forename: S
authors.initials: S
authors.lastname: Ando
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001054:Apolipoproteins A / Q000737:chemistry / Q000378:metabolism; D001055:Apolipoproteins B / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D004524:Eels / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007930:Leucine / Q000378:metabolism; D008074:Lipoproteins / Q000737:chemistry* / Q000378:metabolism; D008079:Lipoproteins, VLDL / Q000737:chemistry; D008099:Liver / Q000378:metabolism*; D008858:Microscopy, Phase-Contrast; D011498:Protein Precursors / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000378:metabolism
chemical_list: D000596:Amino Acids; D001054:Apolipoproteins A; D001055:Apolipoproteins B; D008074:Lipoproteins; D008079:Lipoproteins, VLDL; D011498:Protein Precursors; D011506:Proteins; D007930:Leucine