title: Agrobacterium mediated genetic transformation of chickpea, Cicer arietinum L.
abstract: In leaf and stem explants of chickpea, wild type strains of Agrobacteria were able to induce tumors. These tumors were capable of phytohormone independent growth. A supervirulent strain A281 was found to be most effective. Thus, using an agrobacterium R1601, which carries genes conferring supervirulent phenotype along with a plant selectable marker gene (npt II), transformed calli of chickpea were selected in the presence of 100 micrograms/ml level of kanamycin. Molecular analyses of genomic DNA from transformed calli confirmed the integration of the marker gene into chickpea genome.
authors.affiliation: Biotechnology Centre, Indian Agricultural Research Institute, New Delhi.
authors.lastname: Srinivasan
authors.forename: T
authors.initials: T
authors.lastname: Mohapatra
authors.forename: R P
authors.initials: RP
authors.lastname: Sharma
mesh_terms: D007887:Fabaceae / Q000235:genetics* / Q000382:microbiology; D010946:Plants, Medicinal; D012231:Rhizobium / Q000235:genetics*; D014170:Transformation, Genetic