The project of sequencing the entire Bacillus subtilis genome.
The results obtained during the first year of the project involving the sequencing of the Bacillus subtilis genome are presented. Different gene libraries using a yeast artificial chromosome vector and bacteriophage vectors, lambda FixII and phi 105J124, have been constructed. A total of 300 kbp have been cloned using the lambda FixII vector, 68 kbp of which have been fully sequenced. Several open reading frames showing homologies with genes of other organisms were found. Two genes, previously unknown in this organism, have been identified.
Kunst
F
F
Unité de Biochimie Microbienne, URA 1300, Institut Pasteur, Paris.
Devine
K
K
D001412:Bacillus subtilis / Q000235:genetics / Q000648:ultrastructure*; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000648:ultrastructure*; D004269:DNA, Bacterial / Q000235:genetics / Q000648:ultrastructure*; D005822:Genetic Vectors / Q000502:physiology; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D015003:Yeasts / Q000235:genetics
D004269:DNA, Bacterial