title: Determination of temazepam and temazepam glucuronide by reversed-phase high-performance liquid chromatography.
abstract: A rapid and sensitive method for extracting temazepam from human serum and urine is presented. Free temazepam is extracted from plasma and urine samples using n-butyl chloride with nitrazepam as the internal standard. Temazepam glucuronide is analyzed as free temazepam after incubating extracts with beta-glucuronidase. Separation is achieved using a C8 reversed-phase column with a methanol-water-phosphate buffer mobile phase. An ultraviolet detector operated at 230 nm is used and a linear response is observed from 20 ng/ml to 10 micrograms/ml. The limit of detection is 15.5 ng/ml and the limit of quantitation is 46.5 ng/ml. Coefficients of variation are less than 10% for concentrations greater than 50 ng/ml. Application of the methodology is demonstrated in a pharmacokinetic study using eight healthy male subjects.
authors.affiliation: Department of Pharmacology, Louisiana State University Medical Center, Shreveport 71130-3932.
authors.forename: G W
authors.initials: GW
authors.lastname: Kunsman
authors.forename: J E
authors.initials: JE
authors.lastname: Manno
authors.forename: M A
authors.initials: MA
authors.lastname: Przekop
authors.forename: B R
authors.initials: BR
authors.lastname: Manno
authors.forename: K A
authors.initials: KA
authors.lastname: Llorens
authors.forename: C M
authors.initials: CM
authors.lastname: Kunsman
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013056:Spectrophotometry, Ultraviolet; D013693:Temazepam / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism* / Q000652:urine
chemical_list: C072757:temazepam glucuronide; D013693:Temazepam