Biological characterization of the lytic cycle of actinophage phi A7 in Streptomyces antibioticus.
Some basic parameters of the lytic development of phage phi A7 in Streptomyces antibioticus are described. One-step growth experiments demonstrated that at 28 degrees C phi A7 has a latent period of about 60 min and an exponential growth period of about 35 min. The average burst size ranged from 70-100 plaque forming units per infected cell. At the same temperature 50% of the virions were adsorbed to germ tubes of S. antibioticus in about 10 min. This corresponds to an adsorption constant of 6.5 x 10(-10) ml/min. The phage was unable to adsorb the host at other stages of the life cycle (spores or mycelium). Divalent cations are not required for phi A7 stability but Ca2+ proved to be essential for adsorption and also for a later stage of the vegetative development of the phage.
Diaz
L A
LA
Departamento de Biología Funcional, Universidad de Oviedo, Spain.
Gomez
P
P
Hardisson
C
C
Radicio
M R
MR
D000327:Adsorption; D001435:Bacteriophages / Q000254:growth & development / Q000502:physiology*; D007700:Kinetics; D013302:Streptomyces / Q000502:physiology*; D013997:Time Factors