title: Continuous cell lines from the tick Hyalomma anatolicum anatolicum.
abstract: The establishment of 5 continuous cell lines from embryonic tissues of the tick Hyalomma anatolicum anatolicum is reported. Each line comprises 2 or more cell types; they are maintained at 28 C and 32 C in L-15/H-Lac medium with 20% fetal calf serum, and have been cryopreserved successfully. Sustained and consistent growth was achieved only after 12-41 mo in culture.
authors.affiliation: Centre for Tropical Veterinary Medicine, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian, United Kingdom.
authors.forename: L
authors.initials: L
authors.lastname: Bell-Sakyi
mesh_terms: D000818:Animals; D002460:Cell Line; D015925:Cryopreservation; D005260:Female; D008297:Male; D013987:Ticks / Q000166:cytology*