Isolation and characterization of PC-3 human prostatic tumor sublines which preferentially metastasize to select organs in S.C.I.D. mice.
We have developed and partially characterized a mouse model system for studying human prostate tumor cell metastases in vivo. To develop this model we have selected highly invasive (3 x I.) and non-invasive (3 x N.I.) PC-3 human prostatic tumor sublines based on enhanced and diminished capacities to migrate across a reconstituted basement membrane barrier (Matrigel) in Boyden chamber chemotactic assays. When the 3 x I. cells were injected intravenously (i.v.) in the tail vein of severe combined immune deficient (scid) mice, the cells initially metastasized to a wide variety of tissues as demonstrated by using [125I] IUdR labeled cells and histology. Four distinct sublines were eventually isolated which preferentially metastasized at approximately 80% efficiency to the lumbar vertebrae (PC-3 ML), the mandibular region of the right cheek (PC-3 MC), the rib cartilage (PC-3 MR), and the right front knee bone (PC-3 MK), respectively. Implantation experiments at different sites indicated that organ metastases may somehow be conferred on the tumor subclones by the host tissue.
Wang
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Medical College of Pennsylvania, Department of Pathology, Philadelphia 19129.
Stearns
M E
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D000818:Animals; D001345:Autoradiography; D001859:Bone Neoplasms / Q000473:pathology / Q000556:secondary; D003470:Culture Media / Q000494:pharmacology; D004195:Disease Models, Animal; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009362:Neoplasm Metastasis / Q000473:pathology*; D009368:Neoplasm Transplantation / Q000473:pathology; D011471:Prostatic Neoplasms / Q000473:pathology / Q000556:secondary*; D014407:Tumor Cells, Cultured / Q000473:pathology
D003470:Culture Media