Effect of B- and T-cell mitogens on the maximum activities of hexokinase, lactate dehydrogenase, citrate synthase and glutaminase in bone marrow cells and thymocytes of the rat during four hours of culture.
1. Cells from the bone marrow and cells from the thymus of the rat were incubated in the presence of glucose and glutamine and phytohaemagglutinin, concanavalin-A or lipopolysaccharide. Cells were harvested at times up to 4 hr, extracted and maximum activities of hexokinase, lactate dehydrogenase, citrate synthase or glutaminase measured. 2. In bone marrow cells, there were little changes in enzyme activities except for an increase in the activity of citrate synthase which was prevented by concanavalin-A. This mitogen also caused a decrease in the activity of hexokinase. 3. In contrast, in thymocytes, the activities of hexokinase and glutaminase were decreased in the control condition but addition of lipopolysaccharide, a B-cell mitogen prevented these decreases in activity and concanavalin-A maintained the activity of glutaminase. Concanavalin-A caused a decrease in hexokinase activity but a marked increase in that of glutaminase. 4. It is suggested that changes in the maximum activities of hexokinase and glutaminase over this 4 hr period may represent the effect of removal of thymus-produced growth factors, whose effects can be replaced, at least in part, by two mitogens.
Keast
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Department of Biochemistry, University of Oxford, England.
Newsholme
E A
EA
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects; D001853:Bone Marrow / Q000201:enzymology*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002950:Citrate (si)-Synthase / Q000187:drug effects / Q000378:metabolism*; D003208:Concanavalin A / Q000494:pharmacology; D005972:Glutaminase / Q000187:drug effects / Q000378:metabolism*; D006593:Hexokinase / Q000187:drug effects / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000187:drug effects / Q000378:metabolism*; D008070:Lipopolysaccharides; D008297:Male; D008934:Mitogens / Q000494:pharmacology*; D010835:Phytohemagglutinins; D051381:Rats; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000187:drug effects; D013950:Thymus Gland / Q000166:cytology / Q000201:enzymology*
D008070:Lipopolysaccharides; D008934:Mitogens; D010835:Phytohemagglutinins; D003208:Concanavalin A; D007770:L-Lactate Dehydrogenase; D002950:Citrate (si)-Synthase; D006593:Hexokinase; D005972:Glutaminase