Preparation of a drug-induced allergic hepatic disorder model with penicillin as hapten.
A drug-induced allergic hepatic disorder model was established using a hapten and carrier. Penicillin G was bound to glycine for the preparation of N-hydroxy succinic imidylglycinyl benzylpenicillate (PG-Gly-OSu). Using this as the hapten and liver protein as the carrier, guinea pigs were sensitized with liver protein bound to PG-Gly-OSu. After 2 weeks, the sensitized guinea pigs were directly challenged with hepatocytes bound to PG-Gly-OSu through a mesenteric vein and hepatocellular disorder was induced. When the sensitized guinea pigs were challenged with PG-Gly-OSu alone or with liver protein alone, hepatocellular disorder could not be induced. These results suggest that the combination of PG-Gly-OSu as the hapten and liver protein as the carrier elicits a hepatocellular disorder similar to drug-induced allergic hepatitis.
Mizoguchi
Y
Y
Third Department of Internal Medicine, Osaka City University Medical School, Japan.
Iwasaki
M
M
Tsutsui
H
H
Kobayashi
K
K
Monna
T
T
Morisawa
S
S
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000276:immunology / Q000473:pathology*; D004195:Disease Models, Animal; D004342:Drug Hypersensitivity / Q000276:immunology / Q000473:pathology*; D006168:Guinea Pigs; D006241:Haptens / Q000009:adverse effects* / Q000378:metabolism; D006520:Hepatitis, Animal / Q000139:chemically induced* / Q000276:immunology / Q000473:pathology; D008297:Male; D010406:Penicillins / Q000009:adverse effects* / Q000378:metabolism
D006241:Haptens; D010406:Penicillins