title: Estimation of relative potency with sequential dilution errors in radioimmunoassay.
abstract: Sequential dilution is a very common procedure in radioimmunoassay, in which the dilution error will be accumulated from the highest to the lowest concentration. A simulated example in relative potency determination is used to demonstrate the potentially wrong conclusion that can be drawn, when the dilution error is not properly included in the model. A Bayesian method is used and an alternative approximation via maximum likelihood is proposed. An alternative experimental design is recommended to increase the precision of the inference.
authors.affiliation: Biometrics, CIBA-GEIGY AG, Basel, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Racine-Poon
authors.forename: C
authors.initials: C
authors.lastname: Weihs
authors.forename: A F
authors.initials: AF
authors.lastname: Smith
mesh_terms: D001499:Bayes Theorem; D001699:Biometry; D016013:Likelihood Functions; D015233:Models, Statistical; D011863:Radioimmunoassay / Q000706:statistics & numerical data*