title: Isolation of a human chromosome 14-only somatic cell hybrid: analysis using Alu and LINE-based PCR.
abstract: Interspecific somatic cell hybrids containing single human chromosomes are valuable reagents for localization of cloned genes and DNA fragments to specific chromosomes, for the development of chromosome-specific libraries, and for generation of hybrid cell lines containing subchromosomal regions. A CHO somatic cell hybrid containing a single, intact human chromosome 14 (MHR14) was developed and confirmed by LINE PCR amplification gel pattern, by Alu-517 PCR product dot blot hybridization, and by cytogenetic analysis. MHR14 will serve as the chromosome source for the development of a radiation map of human chromosome 14.
authors.affiliation: Department of Medicine, Baylor College of Medicine, Houston, Texas 77030.
authors.forename: A
authors.initials: A
authors.lastname: Mares
authors.forename: S A
authors.initials: SA
authors.lastname: Ledbetter
authors.forename: D H
authors.initials: DH
authors.lastname: Ledbetter
authors.forename: R
authors.initials: R
authors.lastname: Roberts
authors.forename: J F
authors.initials: JF
authors.lastname: Hejtmancik
mesh_terms: D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002469:Cell Separation; D002871:Chromosome Banding; D002883:Chromosomes, Human, Pair 14; D006224:Cricetinae; D004247:DNA; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004247:DNA