title: Effects of superoxide dismutase on injury induced by anoxia and reoxygenation in equine small intestine in vitro.
abstract: Sheets of mucosa from the jejunum of healthy horses were mounted in incubation chambers and bathed with Krebs-Ringer bicarbonate solution. Changes in tissue function and histologic appearance were compared after the following conditions: (1) control conditions for 30 minutes with 95% O2/5% CO2 in the gas phase; (2) same conditions as control, except incubation with superoxide dismutase (300 U/ml) during the last 18 minutes; (3) anoxia for 15 minutes with 95% N2/5% CO2, followed by reoxygenation for 15 minutes; (4) same conditions as 3, except incubation with superoxide dismutase during reoxygenation; and (5) anoxia for 30 minutes. Anoxia reduced the accumulation of radiolabeled L-alanine and caused cell swelling, as indicated by an increase in tissue water and tissue Na contents. Reoxygenation improved the tissue's ability to accumulate L-alanine, but tissue swelling continued after this treatment. Tissue Na content and L-alanine accumulation were restored to control values by reoxygenation with superoxide dismutase in the bathing medium. The grade of structural damage, as indicated by separation of epithelial cells from villi, was equally severe after all, but control, conditions. Superoxide dismutase had no effect on the tissue control conditions. Results of this study suggest that superoxide radicals are involved in the pathogenesis of reperfusion injury in equine jejunal mucosa and that this may be of clinical importance in cases of small intestinal strangulation obstruction.
authors.affiliation: Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square 19348.
authors.forename: J K
authors.initials: JK
authors.lastname: Johnston
authors.forename: D E
authors.initials: DE
authors.lastname: Freeman
authors.forename: D
authors.initials: D
authors.lastname: Gillette
authors.forename: L R
authors.initials: LR
authors.lastname: Soma
mesh_terms: D000409:Alanine / Q000378:metabolism; D000818:Animals; D046508:Culture Techniques; D005260:Female; D006734:Horse Diseases / Q000188:drug therapy*; D006736:Horses; D000860:Hypoxia / Q000473:pathology / Q000662:veterinary; D007415:Intestinal Obstruction / Q000188:drug therapy / Q000662:veterinary*; D007511:Ischemia / Q000473:pathology / Q000662:veterinary; D007579:Jejunal Diseases / Q000188:drug therapy / Q000662:veterinary*; D007583:Jejunum / Q000098:blood supply; D008297:Male; D010084:Oxidation-Reduction; D015427:Reperfusion Injury / Q000188:drug therapy / Q000662:veterinary*; D013482:Superoxide Dismutase / Q000627:therapeutic use*
chemical_list: D013482:Superoxide Dismutase; D000409:Alanine