Deoxycorticosterone, 18-OH-deoxycorticosterone and corticosterone determination by high performance liquid chromatography in monolayer adrenal cell culture.
Reversed-phase HPLC offers a rapid, qualitative as well as quantitative method for separation and determination of deoxycorticosterone, 18-OH-deoxycorticosterone and corticosterone in primary culture of adrenocortical cells. The resolution is sufficient for the purpose of quantitative determination. The limitation of this method lies in its sensitivity for serum steroid determination, but is perfectly applicable at cell cultures where the concentration of these steroids is highest.
Matilla
M J
MJ
Departamento de Bioquimica y Biología Molecular, Universidad de Málaga, España.
Jiménez
M M
MM
Montiel
M
M
D000311:Adrenal Glands / Q000737:chemistry* / Q000166:cytology; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003345:Corticosterone / Q000032:analysis*; D003900:Desoxycorticosterone / Q000031:analogs & derivatives* / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
C042509:18-hydroxydeoxycorticosterone; D003900:Desoxycorticosterone; D003345:Corticosterone