title: The nuclear coded mitoribosomal proteins YmL27 and YmL31 are both essential for mitochondrial function in yeast.
abstract: Using synthetic oligonucleotides deduced from the N-terminal amino acid sequence of purified mitoribosomal protein (mt r-protein) YmL27, the corresponding nuclear gene MRP-L27 of the yeast Saccharomyces cerevisiae has been cloned and sequenced. The MRP-L27 gene codes for 146 amino acids and is located on chromosome X. The mature YmL27 protein consists of 130 amino acids - after cleaving the putative mitochondrial signal peptide - with a net charge of +17 and a calculated relative molecular mass of 14,798 Da. The YmL27 protein as well as the yeast mitoribosomal protein YmL31, which had been characterized and its gene (MRP-L31) cloned previously, is essential for mitochondrial function as shown by the inability of gene disrupted mutants for the MRP-L27 or MRP-L31 genes to grow on non-fermentable carbon sources.
authors.affiliation: Ruhr-Universität Bochum, Fakultät für Chemie, Lehrstuhl für Biochemie, Germany.
authors.forename: H R
authors.initials: HR
authors.lastname: Graack
authors.forename: L
authors.initials: L
authors.lastname: Grohmann
authors.forename: M
authors.initials: M
authors.lastname: Kitakawa
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D002467:Cell Nucleus / Q000737:chemistry*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D008928:Mitochondria / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D000596:Amino Acids; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; C072325:mitochondrial ribosomal protein YmL27; C050985:ribosomal protein L31