A simple method for the DNA content assay of culture maintained pancreatic islets of Langerhans.
A simple method for assaying DNA content of in vitro culture maintained pancreatic islets is described. By employing lyophilization of the islets and subsequent fluorometric analysis, we have been able to measure DNA from relatively small cell samples. The method may result advantageous, over others, previously reported, since it is less subject to experiment-related interfering conditions. This procedure, which is easy and reliable, even for very low DNA concentrations, seems to be very useful for culture maintained pancreatic islets, since in vitro work conducted with this endocrine tissue is usually associated with small size cell samples. The method may help for a simple assessment of the viable islet cell mass in in vitro studies.
Calafiore
R
R
Istituto di Patologia Speciale Medica, Università degli Studi di Perugia, Italy.
Falorni
A
A
Ambrosini
M V
MV
Basta
G
G
Picchio
M L
ML
Brunetti
P
P
D064753:Ammonium Hydroxide; D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis*; D003902:Detergents; D004285:Dogs; D005260:Female; D005612:Freeze Drying; D006878:Hydroxides; D007515:Islets of Langerhans / Q000737:chemistry*; D008214:Lymphocytes / Q000737:chemistry; D008297:Male; D017830:Octoxynol; D011092:Polyethylene Glycols; D051381:Rats; D011917:Rats, Inbred Lew; D012015:Reference Standards; D013050:Spectrometry, Fluorescence; D013552:Swine
D003902:Detergents; D006878:Hydroxides; D011092:Polyethylene Glycols; D064753:Ammonium Hydroxide; D017830:Octoxynol; D004247:DNA