Characterization of and radioimmunoassay for canine thyroglobulin.
Canine thyroglobulin (cTg) has been isolated and purified. It has similar electrophoretic patterns as Tg from other mammalian species. The main fraction had a MW of 660,000, whereas also fractions of a MW of approximately 1,300,000 (dimer) and 330,000 (subunit) were present. The iodine content was 0.8 to 1.0% (w/w). cTg did not cross-react with antibodies against human Tg to a degree that would allow the use of a radioimmunoassay for human Tg for the determination of cTg in serum or plasma. Therefore a polyclonal antiserum was raised against cTg and a homologous radioimmunoassay was developed, which was sensitive (0.4 micrograms/l) and specific (cross-reactivity in cTg assay of human Tg, goat Tg, T4, T3, and DIT less than 0.01%). Plasma Tg levels in normal dogs of both sexes and aged 3-15 years amounted to 192 +/- 73 micrograms/l (mean +/- SD, n = 30). There was no relation between plasma Tg and T4 levels.
Verschueren
C P
CP
Department of Clinical Sciences of Companion Animals, University of Utrecht, The Netherlands.
Selman
P J
PJ
de Vijlder
J J
JJ
Mol
J A
JA
D000818:Animals; D001323:Autoantibodies / Q000032:analysis; D002850:Chromatography, Gel; D003429:Cross Reactions; D004283:Dog Diseases / Q000097:blood*; D004285:Dogs / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007455:Iodine / Q000032:analysis; D008297:Male; D008970:Molecular Weight; D011237:Predictive Value of Tests; D011863:Radioimmunoassay; D012016:Reference Values; D015203:Reproducibility of Results; D013954:Thyroglobulin / Q000097:blood / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D013964:Thyroid Neoplasms / Q000097:blood / Q000662:veterinary*; D013972:Thyrotropin / Q000494:pharmacology
D001323:Autoantibodies; D013972:Thyrotropin; D013954:Thyroglobulin; D007455:Iodine