[Cloning and sequencing of spinach chloroplast DNA fragments with promoter activities in E. coli].
Sau3A partially digested spinach cDNA fragments were cloned in BgIII restriction site of a E. coli promoter probing vector pGA46. The cloned fragments with promoter activities in E. coli were found by the abilities of recovering tetracycling resistance of their host cells. The promoter functions of the two fragments and their different strengths are deciphered by analyzing their DNA sequences.
Wei
L
L
Institute of Genetics, Academia Sinica, Beijing.
Zhu
L H
LH
Hu
N B
NB
D001483:Base Sequence; D002736:Chloroplasts; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014675:Vegetables
D004247:DNA