Isolation and characterization of a monosialosylgangliopentaosyl ceramide from Xenopus laevis oocyte.
A monosialosylgangliopentaosyl ceramide was isolated from Xenopus laevis oocytes. It represented 5.8% of the total acidic glycosphingolipids. From the results of sugar-composition analysis, enzymatic hydrolysis, permethylation analysis, and negative ion fast atom bombardment mass spectrometry, the structure of the ganglioside was determined to be as follows: [sequence: see text] The predominant species of fatty acids were alpha-hydroxy fatty acids, h22:0, h24:0, and h24:1. The long chain bases of this ganglioside consisted mainly of d18:1 sphingosine and phytosphingosine. Other acidic glycolipids were also characterized. The most abundant component of acidic glycolipids was sulfatide, which represented 85.7% of the total acidic glycolipid mixture. GM3, GM2, GM1a, and GD1a were also detected.
Hidari
K
K
Department of Biochemistry, Faculty of Medicine, University of Tokyo.
Itonori
S
S
Sanai
Y
Y
Ohashi
M
M
Kasama
T
T
Nagai
Y
Y
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D005260:Female; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D006028:Glycosphingolipids; D008969:Molecular Sequence Data; D009865:Oocytes / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014982:Xenopus laevis
D006028:Glycosphingolipids; D005677:G(M1) Ganglioside; C055251:IV(4)-galactosyl-N-acetylganglioside M1b