In vitro assay of nuclear uptake of doxorubicin hydrochloride in osteosarcoma cells of dogs.
A rapid, simple chemosensitivity assay, assessing tumor cell nuclear uptake of doxorubicin hydrochloride, was evaluated in 16 dogs with appendicular osteosarcoma. Doxorubicin was administered to dogs in 5 biweekly treatments, and surgical resection was performed after the second or third treatment. The chemosensitivity assay was performed on biopsy specimens from all dogs before chemotherapy. It was repeated on tissue from resected tumors, and tumors were evaluated histologically to determine the degree of necrosis resulting from chemotherapy. Disease-free and total survival time correlated significantly (P less than 0.05 in both cases) with the degree of postchemotherapy necrosis of the primary tumors. Significant correlation was not apparent between the percentage of tumor cells with nuclear uptake of doxorubicin (in either biopsy or resection samples) and disease-free or total survival time. The percentage of cells with nuclear uptake of doxorubicin in surgically resected tumors correlated significantly (P less than 0.05) with percentage of necrosis.
Weinstein
M J
MJ
Department of Surgery, Foster Hospital for Small Animals, School of Veterinary Medicine, Tufts University, North Grafton, MA 01536.
Berg
J
J
Kusuzaki
K
K
Springfield
D S
DS
Gebhardt
M C
MC
Mankin
H J
HJ
D000818:Animals; D001706:Biopsy / Q000662:veterinary; D004283:Dog Diseases / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004285:Dogs; D004317:Doxorubicin / Q000493:pharmacokinetics* / Q000627:therapeutic use; D005500:Follow-Up Studies; D009336:Necrosis; D012516:Osteosarcoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D014407:Tumor Cells, Cultured
D004317:Doxorubicin