title: Adsorption and expression of penicillin G acylase immobilized onto methacrylate polymers generated with varying pore generating solvent volume.
abstract: Adsorption and expression of penicillin G acylase was studied on macroporous methacrylate polymer beads of differing pore volume, generated with kerosene. The absorption and expression of the penicillin G acylase was dependent on pore volume. Maximum expression of 57% of adsorbed enzyme was obtained on beads synthesized with 40 mL of kerosene, indicating minimum pore-diffusion limitations.
authors.affiliation: Chemical Engineering Division, National Chemical Laboratory, Pune, India.
authors.forename: A
authors.initials: A
authors.lastname: Kotha
authors.forename: L
authors.initials: L
authors.lastname: Selvaraj
authors.forename: C R
authors.initials: CR
authors.lastname: Rajan
authors.forename: S
authors.initials: S
authors.lastname: Ponrathnam
authors.forename: K K
authors.initials: KK
authors.lastname: Kumar
authors.forename: G R
authors.initials: GR
authors.lastname: Ambekar
authors.forename: J G
authors.initials: JG
authors.lastname: Shewale
mesh_terms: D004800:Enzymes, Immobilized / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D007648:Kerosene; D010399:Penicillin Amidase / Q000378:metabolism*; D011109:Polymethacrylic Acids; D016062:Porosity
chemical_list: D004800:Enzymes, Immobilized; D007648:Kerosene; D011109:Polymethacrylic Acids; D010399:Penicillin Amidase