The effect of platelet activating factor on ovulation.
The mechanism of ovulation has been compared to an inflammatory reaction. Platelet activating factor (PAF) is an important mediator of inflammation as it may induce the production of prostaglandins and lysosomal enzyme. We evaluated the potential role of PAF in PMSG-HCG induced ovulation using CV3988, a specific PAF receptor antagonist in a superovulated ICR mice (9-12 weeks old). CV3988 blocked the ovulation in a dose dependent manner, and the significant reduced ovulatory efficiency was observed at more than 500 micrograms dose (p less than 0.001). The ovulatory efficiency reduced by CV3988 was reversed by PAF in a dose dependent manner. In vitro fertilization (IVF) rate of follicular oocytes with treatment of CV3988 was not different from that of ovulated ova without treatment. These results suggest that PAF may be involved in the ovulation process but the presence of PAF may not be essential for the fertilization of the ova as IVF.
Kikukawa
Y
Y
Department of Obstetrics and Gynecology, Asahikawa Medical College, Japan.
Ishikawa
M
M
Sengoku
K
K
Kasamo
M
M
Shimizu
T
T
D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D010064:Embryo Implantation; D005260:Female; D005307:Fertilization in Vitro; D006064:Gonadotropins, Equine / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000187:drug effects*; D010060:Ovulation / Q000187:drug effects / Q000502:physiology*; D010742:Phospholipid Ethers / Q000494:pharmacology*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000502:physiology*; D011247:Pregnancy
D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D010742:Phospholipid Ethers; D010972:Platelet Activating Factor; C037913:CV 3988