Equine cutaneous amyloidosis derived from an immunoglobulin lambda-light chain. Immunohistochemical, immunochemical and chemical results.
Amyloid deposits from equine cutaneous nodular amyloidosis associated with extramedullary plasmacytoma were classified immunohistochemically as equine immunoglobulin lambda-light chain-derived and designated eA lambda (HIP). For chemical identification, the amyloid fibril proteins were separated on Sephadex G-100 in 6M guanidine.HCl. Polypeptides of predominantly 24 kDa and 50 kDa were found by polyacrylamide gel electrophoresis. They have preponderance of immunoglobulin lambda-antigenic determinants as detected by immunodiffusion and immunoblotting. Since the N-terminus of the major proteins was blocked, peptides were generated with trypsin and endoproteinase Asp-N and then isolated using reversed-phase high-performance liquid chromatography. Automatic amino-acid sequence determination of seven peptides showed novel sequences. Data bank comparison indicated that these peptides were derived from a monoclonal immunoglobulin lambda-light and a gamma-heavy chain. The light chain was considered to be the leading amyloidogenic polypeptide, since it was the predominant component in a virtually pure amyloid fibril preparation. Thus, immunoglobulin lambda-light chain-derived amyloidosis, so far established only in man and cat, has now also been identified in the horse.
Linke
R P
RP
Max-Planck-Institut für Biochemie, Martinsried bei München.
Geisel
O
O
Mann
K
K
D000595:Amino Acid Sequence; D000682:Amyloid / Q000032:analysis*; D000686:Amyloidosis / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D002417:Cattle; D006734:Horse Diseases; D006736:Horses; D006801:Humans; D007106:Immune Sera; D007147:Immunoglobulin Light Chains / Q000032:analysis*; D007146:Immunoglobulin lambda-Chains / Q000032:analysis*; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000302:isolation & purification; D010954:Plasmacytoma / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D012689:Sequence Homology, Nucleic Acid; D012867:Skin / Q000276:immunology / Q000473:pathology; D012871:Skin Diseases / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D013552:Swine
D000682:Amyloid; D007106:Immune Sera; D007147:Immunoglobulin Light Chains; D007146:Immunoglobulin lambda-Chains; D010446:Peptide Fragments