A sensitive ELISA for measuring recombinant human interleukin-3 in human plasma or serum.
We describe a sensitive ELISA for the measurement, of the recombinant human cytokine, interleukin-3 (IL-3), in human plasma or serum samples. The assay design uses two different anti-IL-3 monoclonal antibodies, giving a two-site assay configuration. The assay incorporates the use of alkaline phosphatase conjugated to streptavidin and a biotinylated anti-IL-3 monoclonal antibody to amplify the resultant signal. This ELISA can measure both glycosylated and non-glycosylated IL-3. The limits of quantification, as determined by precision profiles and quality control samples prepared in 100% human plasma, are 20 pg/ml and 30 pg/ml for non-glycosylated and glycosylated IL-3, respectively.
Papoian
R
R
Department of Biopharmaceutics, Sandoz Pharma, Ltd., Basel, Switzerland.
Duffy
F
F
Sanner
M
M
Wilt
E
E
D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006031:Glycosylation; D006801:Humans; D066298:In Vitro Techniques; D007377:Interleukin-3 / Q000097:blood* / Q000737:chemistry; D011994:Recombinant Proteins / Q000097:blood
D000911:Antibodies, Monoclonal; D007377:Interleukin-3; D011994:Recombinant Proteins