[The preparation of highly purified insulin and its clinical use. Insulin Cooperative Study Group].
Antigenicity of insulin preparations is obviously related to their purity. The average contents of proinsulin, glucagon and pancreatic polypeptide in highly purified insulin (HP-I) produced by us are 76.0, 2.11 and 0.11 ppm respectively. These results are superior to those for domestic conventional crystalline insulin and single peak insulin (SP-I). The antibodies to insulin, proinsulin and pancreatic polypeptide in serum were examined in 24 diabetic patients treated with HP-I for more than 6 months. The positive rates for the above three antibodies were 37.5%, 0% and 0% respectively. The results showed that the antigenicity of HP-I was much lower than that of domestic conventional crystalline insulin and almost the same as that of MC-I from Nordisk, Denmark.
Yang
J
J
Department of Biochemistry, School of Pharmacy.
Fang
F
F
Zhu
X
X
Deng
S
S
Zhou
W
W
Xing
Z
Z
D001323:Autoantibodies / Q000097:blood; D003920:Diabetes Mellitus / Q000097:blood / Q000188:drug therapy*; D006801:Humans; D007328:Insulin / Q000276:immunology / Q000302:isolation & purification* / Q000627:therapeutic use; D010191:Pancreatic Polypeptide / Q000276:immunology; D011384:Proinsulin / Q000276:immunology
D001323:Autoantibodies; D007328:Insulin; D010191:Pancreatic Polypeptide; D011384:Proinsulin