The gene for a halophilic glutamate dehydrogenase: sequence, transcription analysis and phylogenetic implications.
We have isolated and sequenced the gene for a putative NADP-dependent glutamate dehydrogenase from the extremely halophilic archaebacterium Halobacterium salinarium. This gene is transcribed as a unique RNA molecule of about 1700 nucleotides. The 5' end of the transcript contains characteristic consensus transcription initiation and promoter sequences observed in halophilic archaebacteria. The encoded polypeptide, with a predicted length of 435 amino acids, shows significant overall homology and conservation of functional domains when compared with different eubacterial and eukaryotic glutamate dehydrogenases. Surprisingly, the archaebacterial protein shares a larger number of identical amino acid residues with homologous polypeptides from higher eukaryotes than with those from unicellular eukaryotes and eubacteria.
Benachenhou
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N
Institut de Génétique et Microbiologie, Université Paris-Sud, Orsay, France.
Baldacci
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G
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D005969:Glutamate Dehydrogenase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006217:Halobacterium / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009249:NADP / Q000378:metabolism; D010802:Phylogeny; D011487:Protein Conformation; D014158:Transcription, Genetic
D000596:Amino Acids; D003062:Codon; D009249:NADP; D005969:Glutamate Dehydrogenase