title: Design and in vitro studies of a needle-type glucose sensor for subcutaneous monitoring.
abstract: A new miniaturized glucose oxidase based needle-type glucose microsensor has been developed for subcutaneous glucose monitoring. The sensor is equivalent in shape and size to a 26-guage needle (0.45-mm o.d.) and can be implanted with ease without any incision. The novel configuration greatly facilitates the deposition of enzyme and polymer films so that sensors with characteristics suitable for in vivo use (upper limit of linear range greater than 15 mM, response time less than 5 min, and sensitivity yielding a 5:1 signal-to-background ratio at normal basal glucose levels) can be prepared in high yield (greater than 60%). The sensor response is largely independent of oxygen tension in the normal physiological range. It also exhibits good selectivity against common interferences except for the exogenous drug acetaminophen.
authors.affiliation: Department of Chemistry, University of Kansas, Lawrence 66045.
authors.forename: D S
authors.initials: DS
authors.lastname: Bindra
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: G S
authors.initials: GS
authors.lastname: Wilson
authors.forename: R
authors.initials: R
authors.lastname: Sternberg
authors.forename: D R
authors.initials: DR
authors.lastname: Thévenot
authors.forename: D
authors.initials: D
authors.lastname: Moatti
authors.forename: G
authors.initials: G
authors.lastname: Reach
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D066298:In Vitro Techniques
chemical_list: D005949:Glucose Oxidase; D005947:Glucose