Splenocyte response to T cell-derived cytokines in thymectomized Xenopus.
A miniaturized, "hanging-drop" bioassay reveals that splenocytes from early-thymectomized (Tx) Xenopus can respond (by enhanced thymidine incorporation) to thymic-dependent "cytokines" generated in PHA- or alloantigen-stimulated cultures. Preliminary evidence, using fluorescence activated cell sorting, indicates that surface IgM- splenocytes, rather than sIgM+ cells, from Tx toads are sensitive to the crude, splenocyte-derived, active supernatants. Although these responsive cells display residual, but low, reactivity to PHA, their thymus independence is suggested by flow cytometric observations using the anti-T cell monoclonal antibody XT-1. The development of "T-like" cells in Tx Xenopus is discussed.
Turner
S L
SL
Department of Biological Sciences, University of Durham, U.K.
Horton
T L
TL
Ritchie
P
P
Horton
J D
JD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001681:Biological Assay / Q000379:methods; D002469:Cell Separation; D016207:Cytokines / Q000494:pharmacology*; D005434:Flow Cytometry; D007075:Immunoglobulin M / Q000032:analysis; D007519:Isoantigens / Q000276:immunology; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects*; D013934:Thymectomy; D014981:Xenopus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D016207:Cytokines; D007075:Immunoglobulin M; D007519:Isoantigens; D010835:Phytohemagglutinins; D011947:Receptors, Antigen, B-Cell