Platelet 5-hydroxytryptamine increases with platelet age in dogs.
Thrombocytopenia was induced in mongrel dogs by two mechanisms: immunologically, by intravenous injection of heterologous antiplatelet antibody, and non-immunologically, by circulating the blood through glass beads in anesthetized animals. The platelet content of 5-HT was monitored before and during the recovery of the blood platelet counts. This period is associated with the normalization of the mean platelet survival time and with a progressive increase in the mean age of the circulating platelet population. A continuous increment in platelet 5-HT closely followed the increase in platelet counts in both models of thrombocytopenia, and a strong correlation was found between the platelet age and 5-HT content. These findings support the concept that platelets accumulate 5-HT during their physiological aging process, contradicting the notion that a negative balance in 5-HT content results at the end of their physiological lifespan in circulation. These results are not in conflict with the concept that circulating platelets release and re-uptake 5-HT.
Mezzano
D
D
Department of Hematology-Oncology, School of Medicine, Catholic University, Santiago, Chile.
del Pino
G E
GE
Montesinos
M
M
García
M E
ME
Aranda
E
E
Foradori
A
A
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D001792:Blood Platelets / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D002470:Cell Survival; D004195:Disease Models, Animal; D004285:Dogs; D008297:Male; D012701:Serotonin / Q000097:blood*; D013921:Thrombocytopenia / Q000097:blood / Q000209:etiology / Q000276:immunology
D000906:Antibodies; D012701:Serotonin