title: Selective erythrocyte potassium efflux following pulse treatment with tellurite.
abstract: Human erythrocytes exposed to 0.1 mM tellurite (K2TeO3) in an isotonic buffered choline chloride medium for 15 min at 37 degrees C, washed, and incubated further in the absence of the chemical in the buffer, exhibited selective leakiness for potassium within minutes. The potassium efflux curve was sigmoidal, with an initially slow leakage followed by a sharp rise (first-order kinetics) and a plateau by 60 min. After 15 min, 30-50% of the total potassium concentration had leaked from the cells, although less than 1% lysis had occurred. The control cells incubated in buffer with no K2TeO3 exhibited no potassium leakage. The mean volume of the K2TeO3-treated erythrocytes increased and their median density decreased, indicating changes in the colloid osmotic state and physical characteristics of the cells. However, cells pretreated with K2TeO3 exhibited no significant change in glutathione (GSH) concentration and no membrane lipid peroxidation, unlike cells pretreated with t-butylhydroperoxide (Deuticke et al., Biochim. Bio phys. Acta, 899, 125-128, 1987). The enhanced potassium permeability of the K2TeO3-treated erythrocytes preceded the increase in cell volume, intracellular hydration, and a decrease in median density. We suggest that perturbation of the lipid-protein interaction in the membrane by the oxidant alters the potassium permeability and results in the selective leakage with eventual hemolysis.
authors.affiliation: Department of Medicine, George Washington University Medical Center, Washington, DC 20037.
authors.forename: R K
authors.initials: RK
authors.lastname: Klug
authors.forename: J
authors.initials: J
authors.lastname: Kurantsin-Mills
mesh_terms: D002463:Cell Membrane Permeability / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005978:Glutathione / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D016877:Oxidants / Q000633:toxicity; D011188:Potassium / Q000097:blood*; D013691:Tellurium / Q000633:toxicity*
chemical_list: D016877:Oxidants; D005978:Glutathione; C026660:tellurous acid; D013691:Tellurium; D011188:Potassium