[Acid and basic glycoproteins of human saliva. 2. Investigation of glycoprotein of parotid saliva].
We applied the standard diagnostic electrophoretic method on lyophilized human parotid saliva under the appropriate conditions (pH = 8.6 voltage 90 V and time of 30 seconds). The variation of the essential electrophoretic parameters (volume, time, voltage and pH) gave the best protein separation results in the natural range (pH = 7). Also in all cases, except pH = 11, the catodic side was richer in fractions than the anodic one; this was the qualitative characteristic of the protein component of the parotid saliva. Consequently, the protein content of the parotid saliva was rich in basic elements with the typical electrophoregram and densitogram for human serum and mixed saliva. The Pol-E agarose film method is appropriate for investigation and detection of the protein content in human, especially parotid saliva. It also enables differentiation of samples of mixed and parotid saliva on the basis of appropriate densitograms which are the consequence of different protein and especially glycoprotein components of the content.
Mirković
S
S
University School of Dental Medicine, Belgrade.
D004587:Electrophoresis, Agar Gel; D006023:Glycoproteins / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D010306:Parotid Gland; D012471:Salivary Proteins and Peptides / Q000032:analysis*
D006023:Glycoproteins; D012471:Salivary Proteins and Peptides
Glikoproteini humane pljuvacke. II. Ispitivanje glikoproteina parotidne plkuvacke.