Resistance to antimicrobial agents and prevalence of R plasmids in Pasteurella multocida from swine.
Twenty-nine field isolates of porcine Pasteurella multocida were characterized for their capsular and somatic types and were evaluated for their susceptibility to 10 antimicrobial agents. Plasmid DNA-screening experiments were conducted to determine whether a relationship existed between the presence of plasmids and antibiotic resistance. Field isolates of P multocida were susceptible to most of the antimicrobials tested, but all isolates were resistant to clindamycin. Eleven isolates of serogroup D were resistant to 1 or 2 antimicrobial agents. Resistance to sulfonamides and streptomycin was observed in 7 isolates. These isolates contained R plasmids conferring resistance to streptomycin and sulfonamides. The R plasmids belonged to 2 groups, one of 5.6 kilobase and the other of 5.9 kilobase. Restriction endonuclease mapping and DNA hybridization revealed that these R plasmids were related to RSF1010 from Salmonella panama, which also confers resistance to streptomycin and sulfonamides.
Coté
S
S
Department of Pathology and Microbiology, Faculty of Veterinary Medicine, University of Montreal.
Harel
J
J
Higgins
R
R
Jacques
M
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary; D016979:Pasteurella multocida / Q000187:drug effects* / Q000235:genetics; D011815:R Factors; D012222:Rhinitis, Atrophic / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D015342:DNA Probes; D004269:DNA, Bacterial