Relationship of thyroid status to growth hormone and insulin-like growth factor-I (IGF-I) in plasma and IGF-I mRNA in liver and skeletal muscle of cattle.
Steers were made hyperthyroid or hypothyroid to study the effects of physiological alterations in thyroid hormone status on plasma growth hormone (GH) profiles, plasma insulin-like growth factor-I (IGF-I) concentrations, and relative abundance of IGF-I mRNA in skeletal muscle and liver. Eighteen yearling crossbred steers (360 to 420 kg) were randomly allotted to hyperthyroid (subcutaneous injection 0.6 microgram/kg BW L-thyroxine for 10 d), hypothyroid (oral thiouracil; 0.25% diet plus 12.5 g capsule/d for 17 d), or control (subcutaneous injection 0.9% NaCl) treatment groups. Blood samples were taken for measurement of GH, IGF-I, thyroxine (T4) and triiodothyronine (T3) by RIA. Samples of liver and skeletal muscle were taken by biopsy for measurement of IGF-I mRNA by solution hybridization. Steers receiving thiouracil had 57 and 53% (P less than .05) lower T4 and T3, respectively, than control steers (84.1 and 1.7 ng/ml). The hyperthyroid steers had 228 and 65% greater (P less than .05) T4 and T3 than control steers. Neither increased nor decreased thyroid status had any significant effects on plasma GH profiles, liver IGF-I mRNA, or plasma concentration of IGF-I. There was no effect of thyroid hormone alteration on skeletal muscle IGF-I mRNA concentrations. The results of this study suggest that short-term changes in thyroid status of cattle had no major impact on the GH-IGF-I axis or skeletal muscle IGF-I mRNA.
Hannon
K
K
Department of Animal Science, Iowa State University, Ames 50011.
Trenkle
A
A
D000818:Animals; D002417:Cattle / Q000254:growth & development* / Q000378:metabolism; D013006:Growth Hormone / Q000097:blood*; D007334:Insulin-Like Growth Factor I / Q000032:analysis* / Q000235:genetics; D008099:Liver / Q000737:chemistry; D008297:Male; D009132:Muscles / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis; D011897:Random Allocation; D013889:Thiouracil / Q000494:pharmacology; D013961:Thyroid Gland / Q000502:physiology*; D013963:Thyroid Hormones / Q000097:blood; D013974:Thyroxine / Q000494:pharmacology
D012333:RNA, Messenger; D013963:Thyroid Hormones; D013889:Thiouracil; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D013974:Thyroxine