title: Altered expression of the two naturally occurring human insulin receptor variants in isolated adipocytes of non-insulin-dependent diabetes mellitus patients.
abstract: The human insulin receptor gene is expressed in two variant isoforms which differ by the absence (HIR-A) or presence (HIR-B) of 12 amino acids in the COOH-terminus of the extracellular alpha-subunit as a consequence of alternative splicing of exon 11. Expression of the two variant isoforms is regulated in a tissue-specific manner. In this study, we have measured the levels of the two receptor variants in isolated adipocytes from 10 non-insulin-dependent diabetes mellitus (NIDDM) and 11 normal subjects using an immunological assay, based on the ability of a human anti-receptor autoantibody to discriminate between HIR-A and HIR-B. Results indicate that levels of HIR-B variant are increased in NIDDM patients.
authors.affiliation: Dipartimento di Medicina Interna, Università di Roma Tor Vergata, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Sesti
authors.forename: M A
authors.initials: MA
authors.lastname: Marini
authors.forename: A N
authors.initials: AN
authors.lastname: Tullio
authors.forename: A
authors.initials: A
authors.lastname: Montemurro
authors.forename: P
authors.initials: P
authors.lastname: Borboni
authors.forename: A
authors.initials: A
authors.lastname: Fusco
authors.forename: D
authors.initials: D
authors.lastname: Accili
authors.forename: R
authors.initials: R
authors.lastname: Lauro
mesh_terms: D016475:3T3 Cells; D000273:Adipose Tissue / Q000502:physiology / Q000503:physiopathology*; D000595:Amino Acid Sequence; D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D014644:Genetic Variation; D006801:Humans; D007074:Immunoglobulin G; D007328:Insulin / Q000378:metabolism; D007700:Kinetics; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008875:Middle Aged; D011972:Receptor, Insulin / Q000235:genetics* / Q000378:metabolism; D012016:Reference Values; D014162:Transfection
chemical_list: D007074:Immunoglobulin G; D007328:Insulin; D046911:Macromolecular Substances; D011972:Receptor, Insulin