[Crystallization of free aspartate aminotransferase from chicken heart cytosol].
Homogeneous aspartate aminotransferase (purity--99%, yield--70%) has been prepared from chicken heart cytosol. The purification procedure included fractionation with ammonium sulfate and ethanol and crystallization. Crystals (0.3 x 0.5 x 2 mm) of the free enzyme were prepared from ammonium sulfate solution and studied by X-ray analysis at 2.5 A resolution.
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D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D002645:Chickens; D003460:Crystallization; D003600:Cytosol / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol / Q000737:chemistry; D007525:Isoelectric Focusing; D009206:Myocardium / Q000201:enzymology*; D014961:X-Ray Diffraction
D000431:Ethanol; D001219:Aspartate Aminotransferases; D000645:Ammonium Sulfate
Kristallizatsiia svobodnoĭ aspartataminotransferazy iz tsitozolia serdtsa kur.