Trypanosoma cruzi: incorporation of [3H]-palmitic acid and [3H]-galactose into components shed by trypomastigotes.
Trypomastigotes were metabolically labeled with [3H]-palmitic acid or [3H]-galactose and labeled components were detected in the culture medium. Thin layer chromatography of the shed material showed several lipids in the [3H]-palmitic acid labeled sample while the sugar was mainly incorporated into macromolecules. The material incorporated with the lipidic precursor was fractionated by DEAE-Sephadex (acetate form) and the amount of radioactivity was ten times higher in the acidic lipids than in the neutral lipids. When acidic lipids were further separated by Unisil, 73% of the radioactivity was recovered in the less polar fraction. Different patterns were obtained on comparison of the shed components with the lipids remaining in the parasite.
Couto
A S
AS
Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Uhrig
M L
ML
Agustí
R
R
Befumo
M F
MF
Zingales
B
B
Colli
W
W
de Lederkremer
R M
RM
D000818:Animals; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005690:Galactose / Q000032:analysis / Q000378:metabolism*; D006001:Glycoconjugates / Q000737:chemistry* / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000032:analysis / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000737:chemistry / Q000378:metabolism*
D003470:Culture Media; D006001:Glycoconjugates; D008055:Lipids; D010169:Palmitic Acids; D019308:Palmitic Acid; D005690:Galactose