Uridine fluxes in healthy proliferating T-lymphocytes, MOLT-3 T-ALL cell-line cells and differentiated MOLT-3 cells.
Incorporation of 14C-uridine into UTP and CTP and fluxes of label through these nucleotide pools to RNA and DNA were greater in MOLT-3 cells compared to T-lymphocytes. In growth-arrested, differentiated MOLT-3 cells overall incorporation of radiolabel into nucleotides and nucleic acids was lowered compared to exponentially growing cells. Turnover of UTP and CTP however, retained the profile of exponentially growing MOLT-3 cells, implicating the characteristically higher conversion of UTP to CTP is independent of the MOLT-3 cells proliferative capacities. We conclude that drugs interfering with CTP-synthetase activity are good candidates to be used as selective substances in the battle against T-ALL.
van den Berg
A A
AA
Dept. of Ped. and Clin. Chem., Univ. of Amsterdam, T. Neth.
van Lenthe
H
H
de Korte
D
D
Roos
D
D
van Gennip
A H
AH
D002250:Carbon Radioisotopes; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D002460:Cell Line; D003570:Cytidine Triphosphate / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009696:Nucleic Acids / Q000096:biosynthesis; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma; D011865:Radioisotope Dilution Technique; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014529:Uridine / Q000378:metabolism*; D014544:Uridine Triphosphate / Q000378:metabolism
D002250:Carbon Radioisotopes; D009696:Nucleic Acids; D003570:Cytidine Triphosphate; D013755:Tetradecanoylphorbol Acetate; D014544:Uridine Triphosphate; D014529:Uridine