[Immunosorption of individual HIV proteins and virions].
Immunosorbents specifically binding native (gp160, gp120, gp41) and recombinant env proteins and HIV-I virions were synthesized on the basis of Sepharose 4B and Silica with immobilized ligands such as gamma-fraction of rabbit antiserum to HIV-I proteins and purified antibodies to env proteins of HIV-I. The possibility was shown of selective extraction of HIV-I virions and individual HIV proteins both in vitro and in vivo. The titer of virus antigens (in ELISA) after perfusion via an immunosorbent of patterns with a high content of virions and HIV-I proteins was 8 times as low as the starting titer (after perfusion via the control sorbent it was 2-fold decreased). Extracorporeal immunosorption in animals after intravenous injection of recombinant env protein permitted the latter's titer to be 5 times lower. After perfusion via the control sorbent the titer dropped by at least 20% as compared with the starting titer. The possibility of using immunosorption in multimodality therapy of AIDS is under discussion.
Lopukhin
Iu M
IuM
Pavlenko
V V
VV
Kulaev
D V
DV
Liukova
T K
TK
Petrunin
D D
DD
Karamov
E V
EV
Rudneva
I A
IA
D000163:Acquired Immunodeficiency Syndrome / Q000628:therapy; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D015497:HIV-1 / Q000737:chemistry*; D007163:Immunosorbent Techniques; D007164:Immunosorbents / Q000627:therapeutic use; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000032:analysis*; D014759:Viral Envelope Proteins / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis*; D014771:Virion / Q000737:chemistry*
D007164:Immunosorbents; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins
Immunosorbtsiia individual'nykh bel'kov i virionov VICh.