DNA probes for detection of copper resistance genes in Xanthomonas campestris pv. vesicatoria.
The copper resistance (Cur) genes encoded on pXV10A, a 190-kb plasmid in Xanthomonas campestris pv. vesicatoria XV10, were isolated on a 44-kb cosmid clone designated pCuR1. Tn5 mutagenesis of pCuR1 indicated that a 4.0-kb region was required for copper resistance. Three restriction fragments located within the 4.0-kb region demonstrated high specificity for the Cur genes present in X. campestris pv. vesicatoria and will be useful in monitoring the presence of these genes in the environment.
Garde
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Department of Plant Pathology, Oklahoma State University, Stillwater 74078.
Bender
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D015139:Blotting, Southern; D003001:Cloning, Molecular; D003300:Copper / Q000494:pharmacology*; D015342:DNA Probes; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D010957:Plasmids; D015183:Restriction Mapping; D016959:Xanthomonas campestris / Q000187:drug effects / Q000235:genetics*
D015342:DNA Probes; D003300:Copper