[HLA antigens on erythrocytes. An attempt to determine the origin of HLA-A, B antigens].
HLA sera were absorbed with erythrocytes with proved HLA antigens and blood cells with HLA-A, B antigens absorbed in vitro from serum. Both types of red cells were 5x to 15x washed in saline and exposed for two hours to the action of 15% chloroquine. The degree of absorption of HLA antibodies and the decline of the score were equal or slightly different after absorption with erythrocytes with own or quinked HLA antigens. Washing in saline or processing of blood cells with chloroquine did not affect the resulting absorption. From the results of the experiment ensues that the link of HLA-A, B antigens is equally firm blood cells isolated from the peripheral blood stream and blood cells where the antigens were linked to them in vitro from serum. This suggests that the view is justified that the majority of HLA-A, B antigens detected in blood cells is secondarily linked from plasma.
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Ustav hematologie a krevní transfúze, Praha.
D004912:Erythrocytes / Q000276:immunology*; D006680:HLA Antigens / Q000032:analysis*; D015234:HLA-A Antigens / Q000032:analysis; D015235:HLA-B Antigens / Q000032:analysis; D006801:Humans
D006680:HLA Antigens; D015234:HLA-A Antigens; D015235:HLA-B Antigens
HLA antigeny na erytrocytech. Pokus o zjistĕní původu HLA-A, B antigenů.