title: Intestinal absorption of linoleic acid in experimental renal failure.
abstract: Linoleic acid (LA) transport in rats with experimental short-term and long-term renal failure (RF) was compared with that of sham-operated normal animals on liberal food intake and pair-fed animals. The perfusions in vivo and incubations in vitro were conducted using a micellar solution containing a wide range of LA concentrations. Both absorption in vivo and uptake in vitro of LA were significantly reduced in animals with short-term RF. Lipid extraction and separation by thin-layer chromatography revealed a marked LA trapping as trilinolein (TL) in the perfused intestinal tissue in the short-term RF group. The esterification process, as defined by the rate of LA incorporation into TL, was moderately reduced in short-term RF animals. The thickness of the unstirred water layer showed no significant difference among the groups studied. In contrast, animals with long-term RF exhibited normal absorption of LA in vivo at all concentrations tested. In conclusion, LA absorption is reduced in short-term RF and restored in long-term RF. Several steps including LA transport into and TL transport out of the enterocyte and the esterification process were impaired in short-term RF. These changes are not due to alteration in the unstirred water layer, anorexia, weight loss or a rapid effect of uraemic chemical environment or circulatory factors.
authors.affiliation: Department of Medicine, University of California, Irvine 92668.
authors.forename: M V
authors.initials: MV
authors.lastname: Pahl
authors.forename: A
authors.initials: A
authors.lastname: Barbari
authors.forename: N D
authors.initials: ND
authors.lastname: Vaziri
authors.forename: D
authors.initials: D
authors.lastname: Hollander
authors.forename: M
authors.initials: M
authors.lastname: Yazdani
authors.forename: J
authors.initials: J
authors.lastname: Tran
authors.forename: R
authors.initials: R
authors.lastname: Kan
mesh_terms: D000818:Animals; D007408:Intestinal Absorption / Q000502:physiology*; D007583:Jejunum / Q000737:chemistry / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000378:metabolism*; D008041:Linoleic Acids / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014280:Triglycerides / Q000032:analysis
chemical_list: D008041:Linoleic Acids; D014280:Triglycerides; C009564:trilinolein