Human T lymphocytes require lipid as either lipoprotein or nonesterified fatty acid for in vitro activation.
Serum, which is required for activation of T cells in vitro, was fractionated to isolate and identify major supportive components. The serum activity concentrated exclusively with the lipoprotein fractions LDL and HDL. Furthermore, oleic and linoleic acids were equally active supplements, suggesting that resting T cells may require exogenous lipid because they are incapable of synthesizing fatty acid. A survey of fatty acids disclosed that all were capable of some degree of support. Titration delineated the narrow concentration range of lipid supplements successful in supporting T cell proliferation and demonstrated that concentrations must be rigidly controlled to optimize cell proliferation.
Herzberg
V L
VL
Department of Microbiology, Dartmouth Medical School, Hanover, N.H. 03756.
D000328:Adult; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D005230:Fatty Acids, Nonesterified / Q000276:immunology / Q000378:metabolism*; D006133:Growth Substances / Q000097:blood; D006801:Humans; D008041:Linoleic Acids / Q000378:metabolism; D008074:Lipoproteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism; D008213:Lymphocyte Activation; D009829:Oleic Acids / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D005230:Fatty Acids, Nonesterified; D006133:Growth Substances; D008041:Linoleic Acids; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D009829:Oleic Acids