[In vitro balloon treatment of coronary plaque material of the human: effect of dilation time on proliferation of smooth muscle cells].
The proliferation of smooth muscle cells (SMC) seems to be an important factor in the development of restenosing events after angioplasty. Primary stenosing plaque material was obtained from human coronary arteries by thrombendarterectomy. Specimens were cut in small pieces and dilated in a flexible tube with a balloon-catheter (Medtronic 18K2030E) for 1 min., 5 min. and 10 min. at 3 atm. For the isolation of plaque cells the expant technique was used. The majority of plaque cells was identified as smooth muscle cells by positive reaction with monoclonal antibodies against smooth muscle alpha-actin. For the cultivation of SMC a mixture of Waymouth's MB 752/1 and Ham F-12 (1:1) was used. After 8 weeks cell number was analysed by a cell counter (Casy I, Schärfe System). There was a clear increase of SMC-proliferation in dependence to the duration of angioplasty (1 min. = 100%, 5 min. = 112%, 10 min. = 153%). If these data are confirmed by clinical and experimental studies, the duration of angioplasty has to be considered as a risc factor for the development of restenosis.
Voisard
R
R
Medizinische Klinik IV, Universität Ulm.
Dartsch
P C
PC
Seitzer
U
U
Grupp
C
C
Hannekum
A
A
Kochs
M
M
Hombach
V
V
D015906:Angioplasty, Balloon, Coronary / Q000295:instrumentation*; D002455:Cell Division / Q000502:physiology*; D003324:Coronary Artery Disease / Q000473:pathology* / Q000628:therapy*; D046508:Culture Techniques; D004691:Endarterectomy; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000473:pathology*; D012008:Recurrence
In-vitro Ballonisierung von koronarem Plaquematerial des Menschen: Einfluss der Dilatationsdauer auf die Proliferation der glatten Muskelzellen.