Detection of coliform bacteria and Escherichia coli by multiplex polymerase chain reaction: comparison with defined substrate and plating methods for water quality monitoring.
Multiplex polymerase chain reaction (PCR) and gene probe detection of target lacZ and uidA genes were used to detect total coliform bacteria and Escherichia coli, respectively, for determining water quality. In tests of environmental water samples, the lacZ PCR method gave results statistically equivalent to those of the plate count and defined substrate methods accepted by the U.S. Environmental Protection Agency for water quality monitoring and the uidA PCR method was more sensitive than 4-methylumbelliferyl-beta-D-glucuronide-based defined substrate tests for specific detection of E. coli.
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A K
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Department of Biology, University of Louisville, Kentucky 40292.
McCarty
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SC
Atlas
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RM
D001483:Base Sequence; D004269:DNA, Bacterial; D004587:Electrophoresis, Agar Gel; D004755:Enterobacteriaceae / Q000235:genetics / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D005798:Genes, Bacterial; D006801:Humans; D006923:Hymecromone / Q000031:analogs & derivatives; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014871:Water Microbiology
D004269:DNA, Bacterial; D006923:Hymecromone; C034888:4-methylumbelliferyl glucuronide