Diamines interfere with the transport of L-ornithine in Ehrlich-cell plasma-membrane vesicles.
1. L-Ornithine transport by plasma-membrane vesicles isolated from Ehrlich cells is Na(+)-independent and shows a saturable and a diffusional component. 2. Putrescine, histamine, 5-hydroxytryptamine and 2,3-diaminopropane at 55 microM concentration significantly inhibit 0.5 mM-L-ornithine transport at least for the first 10 min of incubation. 3. There is a trans-stimulatory effect of putrescine on L-ornithine transport.
Medina
M A
MA
Laboratorio de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Málaga, Spain.
Urdiales
J L
JL
Mates
J M
JM
Núñez de Castro
I
I
Sánchez-Jiménez
F
F
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D003959:Diamines / Q000494:pharmacology*; D005973:Glutamine / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D009952:Ornithine / Q000378:metabolism*; D011700:Putrescine / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology
D003959:Diamines; D005973:Glutamine; D012701:Serotonin; C070006:1,2-diaminopropane; D006632:Histamine; D009952:Ornithine; D011700:Putrescine