Multiple xylanases of Cellulomonas fimi are encoded by distinct genes.
Cellulomonas fimi genomic DNA encoding xylanase activity has been cloned and expressed in Escherichia coli. As judged by DNA hybridization and restriction analysis, twelve xylanase-positive clones carried a minimum of four different xylanase (xyn) genes. The encoded enzymes were devoid of cellulase activity but three of the four bound to Avicel.
Clarke
J H
JH
Department of Biochemistry, AFRC Institute of Animal Physiology and Genetics Research, Cambridge, U.K.
Laurie
J I
JI
Gilbert
H J
HJ
Hazlewood
G P
GP
D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D015698:Genomic Library; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism; D006093:Gram-Positive Asporogenous Rods / Q000201:enzymology / Q000235:genetics*; D043325:Xylan Endo-1,3-beta-Xylosidase
D002482:Cellulose; D006026:Glycoside Hydrolases; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase