[Detection of causal agents of AIDS using polymerase chain reaction and chemiluminescence measurement].
The polymerase chain reaction (PCR) allows the detection of minimal amounts of DNA segments, and thus is used for the laboratory diagnosis of HIV (human immunodeficiency virus). In the present study a solution hybridization assay using acridinium ester-labelled probes was applied for detection of amplified HIV-1 DNA segments. Amplification was achieved by 30 cycles of the polymerase chain reaction using SK38/SK39 primers specific for a constant region of the HIV-1 gag region. Amplified products were hybridized with acridinium phenyl ester-labelled probes and measured by chemiluminescence. All of 163 blood samples obtained from HIV-infected patients (CDC II-CDC IV) were reactive, while autoradiography with a 32P-labelled SK19 probe only detected 146 patients (89.5%). The combination of PCR with chemiluminescence avoids the use of radioactive material and is sensitive and quick, allowing the "PCR results" to be reported on the same day.
Schmidt
B L
BL
Ludwig Boltzmann-Institut für dermato-venerologische Serodiagnostik Wien.
Gschnait
F
F
D000386:AIDS-Related Complex / Q000382:microbiology; D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D015342:DNA Probes; D004797:Enzyme-Linked Immunosorbent Assay; D006678:HIV / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D015151:Immunoblotting; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D015342:DNA Probes
Erregernachweis von AIDS mittels Polymerasekettenreaktion und Chemilumineszenzmessung.