title: Factor XIII of blood coagulation inhibits the oxidative phagocyte metabolism and suppresses the immune response in vivo.
abstract: Factor XIII of blood coagulation (F XIII) belongs to the family of transglutaminases and is a major cell product of certain subsets of macrophages. The gene for F XIIIA is coupled to the immune response genes of the HLA-region on chromosome 6. F XIII dose- dependently inhibits the in vitro chemiluminescence response of human phagocytes. About 0.1 units of F XIII/ml (final) decreased the chemiluminescence response to about 50%. In addition, about 0.6 units of F XIII/ml inhibits 50% of the release of the lysosomal hydrolase N-acetyl-beta glucosaminidase in both immune complex stimulated and unstimulated monocytes. Intraperitoneal application of F XIII reduced the activity of phagocytes in a F XIII dose dependent manner. 0.25 units of F XIII reduced the chemiluminescence reaction of murine peritoneal M phi to about 50% of the activity of PBS treated animals after 2 or 24 hours of in vivo incubation. In the Fisher/Lewis rats skin transplantation model, injections of 5 units of F XIII/animal on days 1-7 or on days 10-17 increased the survival times of the transplants from the control value of 17.0 +/- 1.4 to 26.0 +/- 2.0 and 23.0 +/- 2.4 days, respectively. F XIII may represent a novel and physiological immune suppressive agent for a broad range of human diseases of autoimmune character.
authors.affiliation: Department of Hematology, Hospital Virgen del Rocío, University of Seville, Spain.
authors.forename: T W
authors.initials: TW
authors.lastname: Stief
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D003864:Depression, Chemical; D005176:Factor XIII / Q000494:pharmacology*; D005260:Female; D006084:Graft Rejection / Q000187:drug effects; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D008163:Luminescent Measurements; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D010586:Phagocytes / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344 / Q000276:immunology; D011917:Rats, Inbred Lew / Q000276:immunology; D016897:Respiratory Burst / Q000187:drug effects*; D016038:Skin Transplantation
chemical_list: D005176:Factor XIII; D000118:Acetylglucosaminidase