A possible clinical application of multicytokine-producing cytotoxic mononuclear cell (MCCM) therapy.
When peripheral blood mononuclear cells (PBMC) were incubated with a streptococcal preparation, OK-432, for 24 h, PBMC acquired cytolytic activity against cultured and fresh human tumor cells. Such PBMC were called OK-432-activated mononuclear cells (OK-MC). OK-MC produce several kinds of cytokines such as interferon alpha (IFN alpha), IFN gamma, and tumor growth inhibitory factor (TGIF) both in vitro and in vivo. OK-MC-produced cytokines also inhibited the growth of cultured and fresh human tumor cells. The growth inhibition was examined by human tumor clonogenic assay using a double-layer agar technique. The results indicate that two pathways of anti-tumor activity are induced in OK-MC, i.e., cell-mediated and cytokine-mediated.
Katano
M
M
Department of Surgery, Hospital Laboratory, Saga Medical School, Japan.
Kubota
E
E
Yamamoto
H
H
Nakamura
M
M
Matsuo
T
T
Hisatsugu
T
T
Katsuki
T
T
Koga
H
H
Ikezaki
K
K
Tabuchi
K
K
D000230:Adenocarcinoma / Q000473:pathology* / Q000628:therapy; D002051:Burkitt Lymphoma / Q000473:pathology; D003110:Colonic Neoplasms / Q000473:pathology; D016207:Cytokines / Q000378:metabolism* / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006131:Growth Inhibitors / Q000378:metabolism / Q000494:pharmacology; D007155:Immunologic Factors / Q000627:therapeutic use; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism; D010844:Picibanil / Q000494:pharmacology* / Q000627:therapeutic use; D013274:Stomach Neoplasms / Q000473:pathology* / Q000628:therapy; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014410:Tumor Stem Cell Assay
D016207:Cytokines; D006131:Growth Inhibitors; D007155:Immunologic Factors; D010844:Picibanil