Zone electrophoresis of anti-human sperm antibodies in concentration gradients of sucrose.
Zone electrophoresis of anti-human sperm antibodies elicited in chickens and isolated from the yolks of eggs laid by the birds were subjected to zone electrophoresis in sucrose concentration gradients. Because of its very low migration rate in the electric field it was possible to isolate the specific IgY antibody component in a virtually pure state. In zone electrophoresis experiments on human "auto" anti-sperm antibodies the presence of both IgA and IgG types was shown in human Ig. The IgA agglutinating antibody was 1/6 that of the IgG component. The mobility in the electric field of the IgG-type antibody was very low, similar to the IgY-type human sperm antibody.
Polson
A
A
Reproductive Biology Unit, Tygerberg Hospital, Republic of South Africa.
D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D002645:Chickens / Q000276:immunology; D004586:Electrophoresis / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007070:Immunoglobulin A / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry; D007132:Immunoglobulin Isotypes / Q000737:chemistry; D007136:Immunoglobulins / Q000737:chemistry; D008297:Male; D012996:Solutions; D013094:Spermatozoa / Q000276:immunology*; D013395:Sucrose
D001323:Autoantibodies; C016512:IgY; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007136:Immunoglobulins; D012996:Solutions; D013395:Sucrose