[The use of cultured endothelial cells in studies of pathophysiology of diseases].
The endothelial cells comprise a single layer of polygonal cells lining the entire length of blood vessels and the heart. It plays a pivotal role in modulating a number of physiological and pathophysiological processes. This review focuses on the endothelial cell function. To obtain information on endothelial cell functions and its biological behavior, a critical step is studying endothelial cells separated from other types of cells. Methods of isolation, culture and identification of endothelial cells are briefly described. Moreover, there is described a wide variety of applications of cultured endothelial cells in the study of pathophysiology of diseases, and are picked up several points to be solved on this.
Sumiyoshi
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First Department of Pathology, Miyazaki Medical College, Japan.
D000818:Animals; D001161:Arteriosclerosis / Q000209:etiology / Q000473:pathology*; D002463:Cell Membrane Permeability; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology / Q000503:physiopathology; D006801:Humans; D009129:Muscle Tonus; D009362:Neoplasm Metastasis; D010974:Platelet Aggregation; D013927:Thrombosis / Q000209:etiology / Q000473:pathology*