Liquid chromatographic-mass spectrometric analysis for screening of patients with cystinuria, and identification of cystine stone.
Analyses of amino acids in the urine of a normal human and of patients with heterozygous and homozygous cystinuria have been carried out, using liquid chromatography-mass spectrometry with an atmospheric pressure ionization interface system. A kidney cystine stone was also analysed by this system. Very intense quasi-molecular ions ([M + H]+) of standard cystine, arginine, lysine and ornithine were observed on mass chromatograms as base peaks. Mass chromatograms of the urine samples from a normal human and from patients with heterozygous and homozygous cystinuria were easily distinguishable. The retention times in the mass chromatogram and mass spectrum of kidney stone cystine was almost the same as that of authentic cystine.
Watanabe
H
H
Department of Urology, Kochi Medical School, Japan.
Sugahara
K
K
Inoue
K
K
Fujita
Y
Y
Kodama
H
H
D001120:Arginine / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003555:Cystinuria / Q000175:diagnosis* / Q000235:genetics; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007669:Kidney Calculi / Q000737:chemistry*; D008239:Lysine / Q000032:analysis; D013058:Mass Spectrometry; D009952:Ornithine / Q000032:analysis; D012016:Reference Values
D001120:Arginine; D009952:Ornithine; D008239:Lysine