Serotonin-immunoreactivity in the cerebellum of two neurological mutant mice and the corresponding wild-type genetic stocks.
Using a serotonin (5-HT)-specific antibody, we examined the 5-HTergic innervation of the cerebellum in the normal mouse (+/+) and in two neurological mutants: weaver (wv/wv), which are characterized by a genetically determined loss of granule cells, and 'Purkinje cell degeneration' (pcd/pcd), which are characterized by a genetically determined loss of Purkinje cells. In normal cerebellum, serotonin-immunoreactive (5-HT-ir) fibers are discrete and ascend to all three layers of the cerebellar cortex. Serotonin-immunoreactive fibers have a much higher density in the atrophic cerebella of both weaver and pcd mutants, where they form multidirectional contours. These anatomical findings provide a profile of 5-HT axon innervation of mouse cerebellum and extend previous neurochemical observations on the metabolic state of cerebellar 5-HT in neurological mutants.
Triarhou
L C
LC
Department of Pathology, Indiana University School of Medicine, Indianapolis, 46202-5120.
Ghetti
B
B
D000818:Animals; D001369:Axons / Q000737:chemistry / Q000648:ultrastructure; D002525:Cerebellar Cortex / Q000737:chemistry / Q000648:ultrastructure; D002531:Cerebellum / Q000737:chemistry / Q000648:ultrastructure*; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D008818:Mice, Neurologic Mutants; D009410:Nerve Degeneration; D009412:Nerve Fibers / Q000737:chemistry / Q000648:ultrastructure*; D011689:Purkinje Cells / Q000648:ultrastructure; D012701:Serotonin / Q000032:analysis* / Q000276:immunology
D012701:Serotonin