High-performance liquid chromatographic determination of taurine in biological fluids by post-column fluorescence reaction with thiamine.
A high-performance liquid chromatographic method is described for the selective determination of taurine in biological fluids by post-column fluorescence reaction. Taurine was separated on an adsorption-distribution type Shodex Ionpac KC-811 column. Then it was converted with hypochlorite into the corresponding N-chloramine, which was allowed to react with thiamine to give fluorescent thiochrome. As little as 6 ng per injection of taurine could be determined. The average recoveries of spiked taurine in serum and urine were 99.5 +/- 2.7 and 101.8 +/- 2.9%, respectively. The method could be applied to the assay of taurine in human serum and urine with simple pretreatment.
Yokoyama
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T
Central Research Laboratory, SS Pharmaceutical Co. Ltd., Chiba, Japan.
Kinoshita
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T
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D008297:Male; D013654:Taurine / Q000097:blood* / Q000652:urine*; D013831:Thiamine
D013654:Taurine; D013831:Thiamine