Neuroendocrine regulation of osmotic and ionic concentrations in the hemolymph of the freshwater shrimp Macrobrachium olfersii (Wiegmann) (Crustacea, Decapoda).
Putative neuroendocrine mediation of osmotic and ionic responses to acute exposure to high salinity medium was investigated in the freshwater shrimp Macrobrachium olfersii (Wiegmann). Homogenates of supra-esophageal or thoracic ganglia, prepared from shrimps exposed to seawater of 21% S for 6 hr, were injected into the abdominal musculature of shrimps previously exposed to freshwater and subsequently exposed to either freshwater or seawater (21% S). Osmotic, sodium, chloride, potassium, magnesium, and calcium concentrations were determined in hemolymph samples removed by intracardiac puncture at time = 0, 1, 3, or 6 hr after homogenate application. Control shrimps were injected with filtered seawater, isosmotic to the hemolymph, and treated similarly. In control shrimps, the osmotic, Na+, Cl-, K+, Mg2+, and Ca2+ concentrations in the hemolymph increased (P less than or equal to 0.05) after 1-hr exposure to seawater. In shrimps injected with homogenates of supra-esophageal ganglion and exposed to seawater, osmotic and ionic concentrations in the hemolymph did not vary with exposure time; in injected shrimps exposed to freshwater, Na+, Cl-, K+, and Mg2+ concentrations decreased (P less than or equal to 0.05) with time. In shrimps injected with homogenates of thoracic ganglion and exposed to seawater, hemolymph osmotic, K+, and Mg2+ concentrations increased (P less than or equal to 0.05); Na+, Cl-, and Ca2+ concentrations remained unchanged. In injected shrimps exposed to freshwater, hemolymph osmotic concentration alone increased (P less than or equal to 0.05) after 1 hr, all other ionic concentrations remaining unchanged. These data suggest that neurofactors apparently located within the ganglia of the central nervous system of M. olfersii may alter the apparent ionic permeabilities of this shrimp, depending on the salinity characteristics of the external medium. The data support the notion that invasion of the freshwater biotope by estuarine crustaceans has necessitated the evolution of specific physiological mechanisms capable of compensating for the osmotic dilution and ion loss typically encountered by such organisms.
McNamara
J C
JC
Departamento de Biologia, Faculdade de Filosofia, Universidade de São Paulo, Ribeirão Preto, Brasil.
Salomão
L C
LC
Ribeiro
E A
EA
D000704:Analysis of Variance; D000818:Animals; D002118:Calcium / Q000097:blood; D002713:Chlorine / Q000097:blood; D005260:Female; D005728:Ganglia, Sympathetic / Q000502:physiology; D006458:Hemolymph / Q000187:drug effects / Q000502:physiology*; D008274:Magnesium / Q000097:blood; D009490:Neurosecretory Systems / Q000502:physiology*; D033541:Palaemonidae / Q000502:physiology*; D011188:Potassium / Q000097:blood; D012964:Sodium / Q000097:blood; D012965:Sodium Chloride / Q000494:pharmacology*; D013997:Time Factors; D014882:Water-Electrolyte Balance
D012965:Sodium Chloride; D002713:Chlorine; D012964:Sodium; D008274:Magnesium; D011188:Potassium; D002118:Calcium