Bending of a highly repetitive component in rat nuclear DNA.
A highly repetitive component in rat nuclear DNA was isolated by HindIII digestion and cloned. A 370-bp cloned component was highly AT-rich (68.3%) in about one third of the region from the 3'-terminus and showed an anomalously slow gel electrophoretic mobility (k-factor = 1.19). These results indicated that a sequence-directed bending of the helix axis occurs in the component. Accordingly, a subclone containing a tandem dimer of the component was isolated and subjected to a circular permutation analysis for exploring the bend center (1). In consequence, the center was shown to be present in the sequence ranging from position near 270 to the 3'-terminus and estimated to be located around position 340.
Nakamura
K
K
Cell Biology Division, Faculty of Pharmaceutical Sciences, Toyama Medical & Pharmaceutical University, Japan.
Ikeda
Y
Y
Iwakami
N
N
Hibino
Y
Y
Sugano
N
N
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry*; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010957:Plasmids; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid
D004247:DNA; D015247:Deoxyribonuclease HindIII