[Hemorrhagic septicemia of rabbits (rabbit hemorrhagic disease, RHD)--quantitative antigen detection].
The efficiency of a vaccine of inactivated virus is influenced to a great deal by the mass of antigen per dose of application. Therefore it is essential to known the concentration of the antigen. We tested a physical method for quantification of the RHD-Virus. It implies a centrifugation of the prepurified, if necessary, preconcentrated infectious or inactivated virus in a gentle sucrose gradient. It is followed by analysis in a sensitive UV flow-through photometer with a computer calculated virus mass. The extinction coefficient (optical density) of the virus (175S-component) at 254 nm is 3.9 cm2/mg. The haemagglutination test and the ELISA were used to prove the virus specificity of the optical peak and partial for comparing them with the method mentioned above.
Liebermann
H
H
Freidrich-Loeffler-Institut für Tierseuchenforschung Insel Reims.
Bergmann
H
H
Lange
E
E
Schirrmeier
H
H
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D006470:Hemorrhage / Q000276:immunology / Q000662:veterinary*; D011817:Rabbits; D014765:Viral Vaccines / Q000276:immunology*; D014766:Viremia / Q000276:immunology / Q000662:veterinary*
D000956:Antigens, Viral; D014765:Viral Vaccines
Hämorrhagische Septikämie der Kaninchen (Rabbit Haemorrhagic Disease, RHD)--Untersuchungen zum quantitativen Antigennachweis.