Sodium-23 and lithium-7 NMR spin-lattice relaxation measurements in the study intercalation in DNA.
Sodium-23 spin-lattice relaxation rate (the reciprocal relaxation time) measurements have been used to study the intercalation of 9-aminoacridine in calf thymus DNA. The results are analyzed by a two state model based on the counterion condensation theory and a theory for the quadrupolar relaxation of counterions in polyelectrolyte solutions. It is shown that change of the solvent from H2O to D2O has a negligible effect on the intercalation process. Furthermore, an attempt is made to analyze the dependence of the 7Li spin-lattice relation rate on intercalation of 9-aminoacridine in LiDNA. It is shown that both quadrupolar and dipolar mechanisms contribute to the bound 7Li relation rate, and that both these contributions are reduced upon intercalation of 9-aminoacridine.
Hald
M
M
Department of Chemistry, Odense M, Denmark.
Jacobsen
J P
JP
D000585:Aminacrine / Q000737:chemistry; D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry*; D008094:Lithium; D009682:Magnetic Resonance Spectroscopy; D012975:Sodium Isotopes; D013950:Thymus Gland / Q000737:chemistry
D007364:Intercalating Agents; D012975:Sodium Isotopes; D000585:Aminacrine; D004247:DNA; D008094:Lithium