Non-specificity of a colorimetric method for the estimation of N-acetyl-D-glucosamine.
The colorimetric method of Reissig et al. for the estimation of N-acetylamino sugars, is often used as a specific method for the quantification of the N-acetyl-D-glucosamine. Although this assay is more sensitive to the monomer, it recognizes all soluble N-acetyl-D-glucosamine oligomers. This result is very important because this method is extensively used in biology for the estimation of chitinolytic activity.
Domard
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Laboratoire d'Etude des Matériaux Plastiques et des Biomatériaux (URA CNRS 507), Université Claude Bernard, Villeubanne, France.
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D000117:Acetylglucosamine / Q000032:analysis*; D003124:Colorimetry / Q000379:methods*; D012680:Sensitivity and Specificity
D000117:Acetylglucosamine