title: Primary structure and localization of a conserved immunogenic Plasmodium falciparum glutamate rich protein (GLURP) expressed in both the preerythrocytic and erythrocytic stages of the vertebrate life cycle.
abstract: A gene coding for a 220-kDa glutamate rich protein (GLURP), an exoantigen of Plasmodium falciparum, was isolated and its nucleotide sequence was determined. The deduced amino acid sequence contains 2 repeat regions. The sequence of one of these was shown to be conserved among geographically dispersed isolates, and a fusion protein containing that sequence was able to stimulate B- and T-cells. Antibodies against GLURP stained erythrocytic stages of the parasite as well as the hepatic stage as detected by electron microscopy.
authors.affiliation: Department of Infection-Immunology, Statens Seruminstitut, Copenhagen, Denmark.
authors.forename: M B
authors.initials: MB
authors.lastname: Borre
authors.forename: M
authors.initials: M
authors.lastname: Dziegiel
authors.forename: B
authors.initials: B
authors.lastname: Høgh
authors.forename: E
authors.initials: E
authors.lastname: Petersen
authors.forename: K
authors.initials: K
authors.lastname: Rieneck
authors.forename: E
authors.initials: E
authors.lastname: Riley
authors.forename: J F
authors.initials: JF
authors.lastname: Meis
authors.forename: M
authors.initials: M
authors.lastname: Aikawa
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Harada
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D016054:DNA, Protozoan / Q000235:genetics; D004912:Erythrocytes / Q000469:parasitology; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D015800:Protozoan Proteins / Q000235:genetics* / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D015800:Protozoan Proteins; C072492:glutamate-rich protein, Plasmodium