title: Changes in hsp70 alter thermotolerance and heat-shock regulation in Drosophila.
abstract: To test the role of the heat shock protein hsp70 in induced thermotolerance and in the regulation of the heat-shock response, we established cell lines with altered expression of the Hsp70 gene. Underexpressing cells were created by transformation with antisense Hsp70 genes, and overexpressing cells by transformation with extra copies of the wild-type gene. Expression at normal temperatures was achieved by placing Hsp70 coding sequences under the control of the metallothionein promoter. Cells that expressed mutant hsp70s were created by transforming cells with deletion and frameshift mutations. The results indicate that hsp70 plays a major role in both thermotolerance and regulation. Surprisingly, they also indicate that these functions can be separated. Overexpression affected thermotolerance more than regulation; underexpression affected regulation more than thermotolerance. A carboxyl-terminal deletion of Hsp70 had a severe dominant-negative effect on thermotolerance but only a minor effect on regulation; an amino-terminal deletion strongly affected regulation but not thermotolerance. A model that explains these observations is presented.
authors.affiliation: Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637.
authors.forename: J M
authors.initials: JM
authors.lastname: Solomon
authors.forename: J M
authors.initials: JM
authors.lastname: Rossi
authors.forename: K
authors.initials: K
authors.lastname: Golic
authors.forename: T
authors.initials: T
authors.lastname: McGarry
authors.forename: S
authors.initials: S
authors.lastname: Lindquist
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002461:Cell Line, Transformed; D002872:Chromosome Deletion; D003300:Copper / Q000494:pharmacology; D016373:DNA, Antisense; D004331:Drosophila melanogaster / Q000235:genetics*; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D006358:Hot Temperature; D008668:Metallothionein / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D011401:Promoter Regions, Genetic
chemical_list: D016373:DNA, Antisense; D006360:Heat-Shock Proteins; D003300:Copper; D008668:Metallothionein