Micromethod for PMN function studies in neonates.
Polymorphonuclear neutrophils play an important role in host defense mechanism. To assess PMN function in neonate a micromethod using only a few drops of blood was standardised. PMN adherent to coverslip were incubated with bacteria and using a differential staining with Acridine Orange (AO) phagocytic and bactericidal capacity were estimated.
Samtani
J R
JR
Department of Pharmacology, Microbiology and Paediatrics, Seth G. S. Medical College, Parel, Bombay, Maharashtra.
Wagle
S U
SU
Irani
S F
SF
Kshirsagar
N A
NA
D001770:Blood Bactericidal Activity; D006801:Humans; D007231:Infant, Newborn / Q000097:blood* / Q000382:microbiology; D009504:Neutrophils / Q000502:physiology*; D010587:Phagocytosis / Q000502:physiology*