A synthetic mycobacterial heat shock peptide prevents adjuvant arthritis but not proteoglycan-induced synovitis in the rat.
Intradermal immunization of female Lewis rats with 100 micrograms of a nine-amino acid synthetic peptide corresponding to the arthritic T cell-reactive epitope of mycobacterial heat shock protein, three weeks prior to induction of adjuvant arthritis, produced inhibition of day 16 ankle swelling and histologic score. Intraarticular injection of 10 micrograms of bovine articular cartilage proteoglycan monomer emulsified in heavy mineral oil into normal Lewis rat stifle joints produced several hallmarks of chronic synovitis at day 16. Pre-treatment with nonapeptide did not inhibit proteoglycan-induced synovitis. These results indicate that tolerance to the critical epitope of heat shock protein does not abrogate the ability of proteoglycan to induce synovitis in rats.
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Institute of Arthritis and Autoimmunity, Miles Research Center, Miles, Inc., West Haven, CT 06516.
Maniglia
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D000595:Amino Acid Sequence; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000473:pathology / Q000517:prevention & control*; D005260:Female; D006360:Heat-Shock Proteins / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology*; D011509:Proteoglycans; D051381:Rats; D013585:Synovitis / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D006360:Heat-Shock Proteins; D009842:Oligopeptides; D011509:Proteoglycans