Novel oligomannose-type sugar chains derived from glucose oxidase of Aspergillus niger.
The primary structure of the N-linked sugar chains of glucose oxidase from Aspergillus niger was investigated. These sugar chains were released from the polypeptide backbone by hydrazinolysis, and the reducing ends of the sugar chains were pyridylaminated. HPLC of the pyridylamino sugar chains with an amide-silica column showed at least seven sugar chain peaks. Chemical and exoglycosidase digestion and 400 lMHz H-NMR studies of the sugar chains of lower molecular weight showed that these were novel oligomannose-type sugar chains, (Man)5-7 (GlcNAc)2, with the structure: +/- Man alpha 1----3Man alpha 1----3(Man alpha 1----6)Man alpha 1----6(+/- Man alpha 1----3Man alpha 1---3)Man )Man beta 1----4GlcNAc beta 1----4GlcNAc.
Takegawa
K
K
Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan.
Kondo
A
A
Iwamoto
H
H
Fujiwara
K
K
Hosokawa
Y
Y
Kato
I
I
Hiromi
K
K
Iwahara
S
S
D001234:Aspergillus niger / Q000201:enzymology*; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D005949:Glucose Oxidase / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry*
D009844:Oligosaccharides; D005949:Glucose Oxidase; D008358:Mannose