[Isolation of factor IX concentrate from cryosupernatant of donor's plasma].
Soviet anion-exchangers were comparatively studied to specify an optimal carrier useful for isolation of factor ix of the blood coagulation system from donor's blood cryosupernatant. The main criteria of gel selection were the yield and purification degree of factor IX, as well as its specific activity and concentration in eluates. It has been shown that DEAE-agarose 6B-CL, possessing pronounced activity with respect to specific sorption of factor IX, meets these requirements to the greatest degree.
Fetisova
L V
LV
Viazova
E P
EP
Khametova
R N
RN
Shuvalova
A L
AL
Azhigirova
M A
MA
Lazarev
V A
VA
Zeĭnalov
A M
AM
D001782:Blood Donors; D001793:Blood Preservation / Q000379:methods*; D001803:Blood Transfusion; D002852:Chromatography, Ion Exchange / Q000379:methods; D015925:Cryopreservation / Q000379:methods*; D005164:Factor IX / Q000302:isolation & purification* / Q000627:therapeutic use; D002836:Hemophilia B / Q000628:therapy*; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional / Q000379:methods; D008297:Male; D010949:Plasma / Q000737:chemistry*
D005164:Factor IX
Vydelenie kontsentrata faktora IX iz kriosupernatanta donorskoĭ plazmy.