[Determination of cell nuclear DNA content in polyps and cancerous degeneration in the large intestine].
Nuclear DNA content was measured by microspectrophotometry in 40 biopsy specimens from patients with large bowel polyps (10 juvenile polyps and inflammatory polyps, 25 adenoma subdivided into 3 groups with 10 Grade I, 10 Grade II and 5 Grade III, 5 cancerous degeneration), and 5 normal epithelium of large intestine. The mean DNA value increased steadily as follows: normal epithelium of intestine (13.54 +/- 1.76 Au); juvenile polyps and inflammatory polyps (14.89 +/- 0.40 Au); adenoma Grade III (21.96 +/- 0.92 Au); cancerous degeneration (24.47 +/- 1.48 Au). The difference in DNA value in these groups was statistically significant (P less than 0.05-0.01). These results suggest that measurement of nuclear DNA content may serve as an objective quantitative parameter for diagnosis of large bowel carcinomatous change of polyps and classification of polypoid adenomas.
Liu
F X
FX
First Hospital, Third Army Medical College, Chongqing, Sichuan.
D000236:Adenoma / Q000737:chemistry; D003111:Colonic Polyps / Q000737:chemistry*; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D007417:Intestinal Polyps / Q000737:chemistry*; D015591:Microspectrophotometry; D012004:Rectal Neoplasms / Q000737:chemistry*
D004273:DNA, Neoplasm