Dextran sulphate reduces diphenylhexatriene anisotropy in human peripheral blood lymphocytes: impact on plasma membrane fluidity and HIV-cytopathogenicity.
Cytopathogenicity of HIV and other enveloped viruses is reduced by membrane fluidizing agents and by dextran sulphate (DS). To investigate whether DS exerts its antiviral action via plasma membrane fluidization of host cells, we performed anisotropy measurements on human peripheral blood lymphocytes (PBL) using the fluorescent marker diphenylhexatriene. Anisotropy was decreased in DS-exposed PBL indicating increased fluidity in the hydrophobic membrane interior.
Lehr
H A
HA
Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
Zimmer
J P
JP
Hübner
C
C
Reisinger
E C
EC
Kohlschütter
A
A
Schmitz
H
H
D000328:Adult; D015704:CD4 Antigens / Q000276:immunology; D002462:Cell Membrane / Q000187:drug effects* / Q000502:physiology; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D016264:Dextran Sulfate / Q000494:pharmacology*; D004161:Diphenylhexatriene; D004357:Drug Synergism; D005260:Female; D015497:HIV-1 / Q000187:drug effects / Q000472:pathogenicity*; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects* / Q000502:physiology; D013050:Spectrometry, Fluorescence; D014343:Trypan Blue
D015704:CD4 Antigens; D004161:Diphenylhexatriene; D016264:Dextran Sulfate; D014343:Trypan Blue