Sites and efficacy of photodamage by tin etiopurpurin in vitro using different delivery systems.
Photosensitization by tin etiopurpurin (SnET2) was determined in cell culture using sensitizer dissolved in ethanol or solubilized via three different delivery systems: Cremophor EL, gamma-cyclodextrin or Molecusol (a more water-soluble cyclodextrin derivative). Sensitizer uptake was substantially more efficient when the delivery systems were employed, in terms of intracellular level needed to lethally photosensitize cells. We observed photodamage at both membrane and mitochondrial loci, but the former was better correlated with loss of viability.
Kessel
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Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201.
Morgan
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Garbo
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GM
D000818:Animals; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D007939:Leukemia L1210; D008074:Lipoproteins / Q000378:metabolism; D051379:Mice; D011166:Porphyrins / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D014001:Tin / Q000378:metabolism / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D008074:Lipoproteins; D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; C063166:tin etiopurpurin; D014001:Tin