title: An improved method for purifying antihemorrhagic factor in the serum of Habu snake (Trimeresurus flavoviridis).
abstract: A new method is presented for purifying antihemorrhagic factor in the serum of Japanese Habu snake (Trimeresurus flavoviridis). The method consists of specific binding of the factor to a hemorrhagic principle-conjugated Sepharose, gel filtration and reverse-phase HPLC. The improved method is simple and rapid, providing the factor with a great increase in specific activity and in a high yield.
authors.affiliation: Department of Applied Immunology, National Institute of Health, Tokyo.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamakawa
authors.forename: T
authors.initials: T
authors.lastname: Omori-Satoh
mesh_terms: D000818:Animals; D000997:Antivenins / Q000302:isolation & purification*; D001798:Blood Proteins / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006470:Hemorrhage / Q000188:drug therapy; D007525:Isoelectric Focusing; D011485:Protein Binding; D012911:Snakes
chemical_list: D000997:Antivenins; D001798:Blood Proteins; C003941:antihemorrhagic factor