Profilin and profilin mRNA in the cerebellum of the developing rat.
Profilin and profilin mRNA concentrations were determined in the cerebellum of the developing rat. Profilin represented nearly 0.1% of total Triton-soluble protein both at birth and at the end of the developmental period. During brain maturation, the profilin concentration was regulated at the transcriptional level. A transient increase of profilin mRNA and profilin was observed at the end of the first postnatal week, a period of intense cell proliferation, neuritic outgrowth and synaptogenesis. We propose that profilin may act by forming, during the critical period of cerebellar development, a reserve pool of monomeric actin that can easily be mobilized in cell regions such as growth cones or synaptic junctions where filamentous actin is highly concentrated.
Léna
J Y
JY
Laboratoire de Neurobiologie Endocrinologique, URA 1197 CNRS, Université Montpellier II-Sciences et Techniques du Languedoc, France.
Sri Widada
J
J
Ferraz
C
C
Liautard
J P
JP
Rabié
A
A
Faivre-Sarrailh
C
C
D000199:Actins / Q000378:metabolism; D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern; D002531:Cerebellum / Q000254:growth & development / Q000378:metabolism*; D003285:Contractile Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D051304:Profilins; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000199:Actins; D003285:Contractile Proteins; D008840:Microfilament Proteins; C495740:Pfn1 protein, rat; D051304:Profilins; D012333:RNA, Messenger