The tissue distribution of rat chromogranin A-derived peptides: evidence for differential tissue processing from sequence specific antisera.
The distribution of chromogranin A and related peptides in rat tissues was investigated using sequence specific antisera. N- and C-terminal antisera and a presumptive C-terminal rat pancreastatin antiserum immunostained an extensive neuroendocrine cell population throughout the gastro-entero-pancreatic tract, anterior pituitary, thyroid and all adrenomedullary cells. However, mid- to C-terminal antisera immunostained a subpopulation of chromogranin A positive cells. Most notable of these was with the KELTAE antiserum (R635.1) which immunostained discrete clusters of adrenomedullary cells and antiserum A87A which immunostained a subpopulation of cells in the anterior pituitary and throughout the gastrointestinal tract. The present study has demonstrated the widespread occurrence of chromogranin A and related peptides in rat neuroendocrine tissues and provides evidence of tissue and cell specific processing.
Curry
W J
WJ
Department of Medicine, Queen's University of Belfast, N. Ireland, UK.
Johnston
C F
CF
Hutton
J C
JC
Arden
S D
SD
Rutherford
N G
NG
Shaw
C
C
Buchanan
K D
KD
D000313:Adrenal Medulla / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis*; D007106:Immune Sera; D007150:Immunohistochemistry; D007422:Intestines / Q000737:chemistry; D007515:Islets of Langerhans / Q000737:chemistry; D008297:Male; D008969:Molecular Sequence Data; D010187:Pancreatic Hormones / Q000032:analysis; D010280:Parathyroid Glands / Q000737:chemistry; D010903:Pituitary Gland, Anterior / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000737:chemistry; D013961:Thyroid Gland / Q000737:chemistry
D053379:Chromogranin A; D002864:Chromogranins; D007106:Immune Sera; D010187:Pancreatic Hormones; C506153:chromogranin A, rat; C050836:pancreastatin