[Aging effects on the light response of the interphotoreceptor matrix as revealed by binding of Ricinus communis agglutinin-1].
This study intended to explore whether the light response of the interphotoreceptor matrix (IPM) is affected by aging. The binding pattern of fluorescence-labeled Ricinus communis agglutinin-1 (RCA) to IPM was examined histochemically in 2 month-old and 1.5 year-old rats under light- and dark-adapted conditions. Two month-old animals showed obvious light-evoked changes in the rod associated IPM: the photoreceptor inner segment zone showed a greater fluorescence than the outer segment zone in the light, whereas the staining-intensity of the former was less than that of the latter in the dark. On the other hand, 1.5 year-old rats did not show such light-evoked IPM responses as in 2 month-old animals: no light-dark differences were found in RCA-1 binding. The scarce, linear, preferential binding of RCA-1 to the cone-associated IPM was the same in both lighting conditions independent of the age.
Uehara
F
F
Department of Ophthalmology, Kagoshima University Faculty of Medicine, Japan.
Ohba
N
N
Yasumura
D
D
LaVail
M M
MM
D000375:Aging / Q000502:physiology*; D000818:Animals; D005109:Extracellular Matrix / Q000378:metabolism / Q000502:physiology; D006651:Histocytochemistry; D037102:Lectins / Q000378:metabolism*; D008027:Light; D010775:Photic Stimulation; D010786:Photoreceptor Cells / Q000378:metabolism / Q000502:physiology*; D037121:Plant Lectins; D051381:Rats; D011919:Rats, Inbred Strains
D037102:Lectins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1