Use of a mutant strain of the cyanobacterium Synechococcus R2 for the determination of nitrate.
A sensitive procedure for the determination of nitrate within the 1- to 50-microM range is described. The method is based on the photoreduction of nitrate to nitrite by whole cells of a nitrite reductase-less mutant strain of the unicellular cyanobacterium Synechococcus R2. Cell suspensions of this cyanobacterium retain high levels of nitrate photoreduction activity for at least 2 months when maintained in the presence of dimethyl sulfoxide at -70 degrees C.
Madueño
F
F
Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla-CSIC, Spain.
Guerrero
M G
MG
D015925:Cryopreservation / Q000379:methods; D000458:Cyanobacteria / Q000235:genetics / Q000378:metabolism*; D004121:Dimethyl Sulfoxide; D009154:Mutation; D009566:Nitrates / Q000032:analysis* / Q000378:metabolism; D009572:Nitrite Reductases / Q000235:genetics; D009573:Nitrites / Q000378:metabolism; D010788:Photosynthesis / Q000502:physiology
D009566:Nitrates; D009573:Nitrites; D009572:Nitrite Reductases; D004121:Dimethyl Sulfoxide