Preferential stimulation by glucose of its oxidation relative to glycolysis in purified insulin-producing cells.
A rise in extracellular D-glucose concentration increases to a greater relative extent the conversion of both D-[5-3H]glucose to 3HOH and D-[6-14C]glucose to 14CO2 in rat purified insulin-producing cells than previously observed in pancreatic islets. In the pure B-cells, the ratio between D-[6-14C]glucose oxidation and D-[5-3H]glucose utilization increases, in a sigmoidal manner, as a function of the hexose concentration. The preferential stimulation by D-glucose of mitochondrial oxidative events is proposed to represent an unusual but essential feature of the metabolic and, hence, functional response of these fuel-sensor cells.
De Vos
A
A
Department of Biochemistry, Vrije Universiteit Brussel, Belgium.
Schuit
F C
FC
Malaisse
W J
WJ
D000105:Acetyl Coenzyme A / Q000378:metabolism; D000818:Animals; D002469:Cell Separation; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D006019:Glycolysis / Q000187:drug effects; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats
D000105:Acetyl Coenzyme A; D005947:Glucose