title: Elimination of caffeine interference in high-performance liquid chromatographic determination of cotinine in human plasma.
abstract: A high-performance liquid chromatographic method with ultraviolet photometric detection for the determination of cotinine in human plasma was described. The use of a 30-cm reversed-phase column and of a mobile phase consisting of water-methanol-0.1 M sodium acetate-acetonitrile (72:21:5.6:1.4, v/v), pH 4.1, eliminated caffeine interference. A simplified solid-phase extraction procedure was also performed for plasma samples.
authors.affiliation: Clinical Biochemistry Department, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Pichini
authors.forename: I
authors.initials: I
authors.lastname: Altieri
authors.forename: R
authors.initials: R
authors.lastname: Pacifici
authors.forename: M
authors.initials: M
authors.lastname: Rosa
authors.forename: P
authors.initials: P
authors.lastname: Zuccaro
mesh_terms: D002110:Caffeine / Q000032:analysis / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003367:Cotinine / Q000032:analysis / Q000097:blood*; D004347:Drug Interactions; D006801:Humans
chemical_list: D002110:Caffeine; D003367:Cotinine