Binding studies and localization of Escherichia coli lipopolysaccharide in cultured hepatocytes by an immunocolloidal-gold technique.
In this study, the uptake and localization of an Escherichia coli lipopolysaccharide, and the temperature effects on these processes, were studied in rat cultured hepatocytes using a binding assay and an immunocolloidal gold technique. The lipopolysaccharide was found to bind to the cell membrane and microvilli after short incubation times, at both 4 degrees C and 37 degrees C. This was followed by a dispersed localization into the cytoplasm, reaching mitochondria. The uptake was found not to be receptor-mediated. A decrease of temperature, delays, but does not prevent, the lipopolysaccharide internalization.
Díaz-Laviada
I
I
Department of Biochemistry and Molecular Biology, Faculty of Chemistry, Universidad Complutense, Madrid, Spain.
Ainaga
J
J
Portolés
M T
MT
Carrascosa
J L
JL
Muncio
A M
AM
Pagani
R
R
D000818:Animals; D001665:Binding Sites; D002478:Cells, Cultured; D004926:Escherichia coli / Q000737:chemistry*; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000032:analysis*; D008099:Liver / Q000382:microbiology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D008070:Lipopolysaccharides