Phospholipase A2 (PLA2) activity in bovine pulmonary artery endothelial cells.
We have investigated the role of recombinant human interleukin-1 beta (rIL-1 beta) and recombinant human tumor necrosis factor alpha (rTNF-alpha) on PLA2 activity, protein synthesis and eicosanoid production in bovine pulmonary artery endothelial cells. Cellular PLA2 activity increased 4-fold and production of PGE2 increased 3-fold at 1-2 hrs in the presence of 10 units/ml rIL-1 beta. PLA2 activity increased 3-fold at 30 min and PGE2 production increased 2-fold with 5 x 10(-9) M rTNF-alpha. The data show that endothelial cells respond more rapidly to rIL-1 beta (2-6 hr) and rTNF-alpha (30 min) than do chondrocytes and synovial cells (6-16 hrs), suggesting endothelial cells may play a primary role in initiating the inflammatory response.
Goodman
R
R
Du Pont Merck Pharmaceutical Company, Wilmington, DE 19898.
Stevens
T M
TM
Mantegna
L R
LR
Kidd
P R
PR
Harris
R R
RR
Kerr
J S
JS
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis; D015777:Eicosanoids / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000201:enzymology*; D007375:Interleukin-1 / Q000494:pharmacology; D009124:Muscle Proteins / Q000096:biosynthesis; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D011651:Pulmonary Artery / Q000201:enzymology; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D015777:Eicosanoids; D007375:Interleukin-1; D009124:Muscle Proteins; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D010741:Phospholipases A; D054467:Phospholipases A2; D015232:Dinoprostone