title: Specific detection of the toxic shock syndrome toxin-1 gene using the polymerase chain reaction.
abstract: A rapid and specific assay for toxic shock syndrome toxin-1 gene (tst gene) detection in Staphylococcus aureus was developed using the polymerase chain reaction. A two-primer set and an oligonucleotide detection probe were synthesized. After 40 cycles of amplification, detection of a 160-bp amplified DNA fragment was carried out by agarose gel electrophoresis and Southern blot hybridization. This assay was sensitive since it was able to detect 1-10 bacteria. It was also specific since no amplification was documented with DNAs from enterotoxigenic S. aureus or Gram-negative bacteria devoid of the tst gene.
authors.affiliation: Institut de Bactériologie, Faculté de Médecine, France.
authors.forename: B
authors.initials: B
authors.lastname: Jaulhac
authors.forename: G
authors.initials: G
authors.lastname: Prevost
authors.forename: Y
authors.initials: Y
authors.lastname: Piemont
mesh_terms: D001427:Bacterial Toxins; D015342:DNA Probes; D004768:Enterotoxins / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012772:Shock, Septic / Q000235:genetics*; D013203:Staphylococcal Infections / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification; D018089:Superantigens
chemical_list: D001427:Bacterial Toxins; D015342:DNA Probes; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal