Isolation of mono- and di-iodine 125 tyramines for conjugation labelling.
Here we describe a method for isolating mono- and di-iodine 125 tyramines from a crude tyramine radio-iodination reaction mixture. Tyramine was radio-iodinated according to a modified chloramine-T method. Mono- and diforms were separated from each other, as well as from free 125I and unreacted tyramine, by means of paper electrophoresis or thin-layer chromatography. Both 125I-tyramine derivatives finally obtained were pure and had high specific activities, which averaged 2500 Ci/mmol for mono-125I-tyramine and 5000 Ci/mmol for di-125I-tyramine. Due to their characteristics, these derivatives can be used for labelling various substances indirectly and preparing in vivo and in vitro tracers of high purity and specific activity, when conjugation labelling is required.
Kakabakos
S E
SE
NCSR Demokritos, Institute of Radioisotopes/Radiodiagnostic Products, Athens, Greece.
Livaniou
E
E
Evangelatos
S A
SA
Evangelatos
G P
GP
Ithakissios
D S
DS
D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D014439:Tyramine
D007457:Iodine Radioisotopes; D014439:Tyramine