A model for in vivo serial investigation of hepatic metabolism using 31P nuclear magnetic resonance spectroscopy.
We have developed an animal model which allows serial, in vivo, evaluation of high energy phosphate metabolism in rat liver, using 31P nuclear magnetic resonance spectroscopy (MRS). In seven rats, the left lobe of the was surgically relocated into a subcutaneous position. The animals recovered quickly from the surgery and continued to thrive. Localization of the liver was obtained using simple surface coil technology. Typical MRS data acquisition times were less than 45 minutes. After the 28 days, the livers showed both normal histology and 31P metabolic measures.
Thompson
R T
RT
Department of Nuclear Medicine, Victoria Hospital, University of Western Ontario, London, Canada.
Schneeberger
A L
AL
Marsh
G D
GD
Driedger
A A
AA
Inculet
R I
RI
D000375:Aging; D000818:Animals; D001835:Body Weight; D004734:Energy Metabolism; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000254:growth & development / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D024510:Muscle Development; D009132:Muscles / Q000378:metabolism; D010758:Phosphorus; D051381:Rats; D011916:Rats, Inbred F344; D012016:Reference Values
D010758:Phosphorus