title: Ovine trophoblast protein-one: evidence for possible glycosylation.
abstract: 1. The polymerase chain reaction has been used to amplify specifically the cDNA coding for the secreted form of ovine trophoblast protein-one from a preparation of total cellular RNA extracted from sheep embryos removed from ewes 16 days after mating. 2. Cloning and sequencing of the amplified cDNA revealed two new sequence variants: SPW49 having 93% similarity with deduced amino acid sequences from published cDNA data, and SPW27 a variant coding for a deleted form of ovine trophoblast protein-one. 3. The gene for ovine trophoblast protein-one is intronless. 4. This study provides further evidence for the existence of an ovine trophoblast protein-one gene family. 5. Both variants contain a potential N-glycosylation site not apparent in published sequences for ovine trophoblast protein-one.
authors.affiliation: Department of Biochemistry and Physiology, University of Reading, Whiteknights, England.
authors.forename: S P
authors.initials: SP
authors.lastname: Watkins
authors.forename: M K
authors.initials: MK
authors.lastname: Jeacock
authors.forename: D
authors.initials: D
authors.lastname: Savva
authors.forename: D A
authors.initials: DA
authors.lastname: Shepherd
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D006031:Glycosylation; D007370:Interferon Type I; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011257:Pregnancy Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D012756:Sheep
chemical_list: D007370:Interferon Type I; D011257:Pregnancy Proteins; C020947:interferon tau; D004247:DNA