title: Evidence for human DNA-mediated transfer of the suppressed phenotype into malignant Chinese hamster cells.
abstract: Genetic suppression of the neoplastic phenotype has been demonstrated in somatic cell hybrids between tumor and normal cells. Suppression in whole-cell and microcell hybrids cannot, as yet, be attributed to specific elements defined at the molecular level. To identify a gene capable of suppressing the neoplastic phenotype, we have introduced DNA of normal human cells into tumorigenic Chinese hamster Wg3-h-o cells. Primary and secondary transfectants which exhibit the suppressed phenotype similar to Wg3-h-o x embryonic fibroblast hybrids were selected. The cells require serum growth factors and anchorage for proliferation in vitro and show a reduced tumorigenicity in nude mice. Transferred human DNA segments were molecularly cloned from a secondary transfectant. Indirect evidence suggests that the cloned human DNA is associated with the expression of the suppressed phenotype.
authors.affiliation: Division of Cancer Research, Institute of Pathology, University of Zürich, Switzerland.
authors.forename: R
authors.initials: R
authors.lastname: Schäfer
authors.forename: A C
authors.initials: AC
authors.lastname: Nirkko
authors.forename: P M
authors.initials: PM
authors.lastname: Ambühl
authors.forename: K H
authors.initials: KH
authors.lastname: Grzeschik
authors.forename: I
authors.initials: I
authors.lastname: Schwarte-Waldhoff
mesh_terms: D016475:3T3 Cells; D000818:Animals; D015139:Blotting, Southern; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000032:analysis / Q000235:genetics*; D005260:Female; D006801:Humans; D051379:Mice; D010641:Phenotype; D010920:Placenta / Q000502:physiology; D011247:Pregnancy; D013489:Suppression, Genetic; D014162:Transfection
chemical_list: D004247:DNA