Modulation of epidermal growth factor binding to receptor by isoproterenol and cholera toxin in primary cultured hepatocytes.
The treatment of rat hepatocytes with isoproterenol induced the increase in the affinity of high affinity binding sites and the decrease in the number of low affinity binding sites of epidermal growth factor (EGF). Similar results were obtained from the pretreatment of hepatocytes with cholera toxin. These results suggest that adenylate cyclase affects the affinity and number of EGF-receptor in hepatocytes.
Katoh
S
S
Department of Radiopharmacy, Tohoku College of Pharmacy, Sendai, Japan.
Kohno
H
H
Ohkubo
Y
Y
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D004815:Epidermal Growth Factor; D002772:Cholera Toxin; D066246:ErbB Receptors; D000262:Adenylyl Cyclases; D007545:Isoproterenol