Nucleotide sequence of a human heart cDNA encoding the mitochondrial phosphate carrier.
We have isolated and characterized a full length cDNA clone encoding the precursor of the human heart mitochondrial phosphate carrier protein. The entire clone is 1330 bp in length with 5'- and 3'-untranslated regions of 48 and 184 bp, respectively. The open reading frame encodes the mature protein consisting of 312 amino acids, preceded by a presequence of 49 amino acids. The amino acid sequence of the mature human phosphate carrier is 93.6, 94.2 and 33.6% identical to that of the phosphate carrier from beef, rat and yeast, respectively. Like other mitochondrial transport proteins, the human phosphate carrier has a tripartite structure. Each of the three repeats contains two hydrophobic regions which presumably span the membrane in the form of alpha-helices.
Dolce
V
V
Department of Pharmaco-Biology, University of Bari, Italy.
Fiermonte
G
G
Messina
A
A
Palmieri
F
F
D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D004272:DNA, Mitochondrial; D006801:Humans; D008929:Mitochondria, Heart / Q000378:metabolism*; D008969:Molecular Sequence Data; D028044:Phosphate-Binding Proteins; D010710:Phosphates / Q000378:metabolism*; D011498:Protein Precursors / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D002352:Carrier Proteins; D004272:DNA, Mitochondrial; D028044:Phosphate-Binding Proteins; D010710:Phosphates; D011498:Protein Precursors