Electrochemical detection of proteins in high-performance liquid chromatography using on-line, postcolumn photolysis.
Direct detection of proteins in high-performance liquid chromatography electrochemistry (LCEC) is difficult. By using on-line, postcolumn photolysis, proteins now can be detected by LCEC at microgram per milliliter levels. The compatibilities of size exclusion chromatography (SEC), reversed-phase chromatography (RPC), ion-exchange chromatography (IEC), and hydrophobic interaction chromatography (HIC) with photolysis-electrochemical detection is described for proteins together with the analytical figures of merit. Inherent from the advantages of electrochemical detection, the method is sensitive and selective.
Dou
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Department of Chemistry, Northeastern University, Boston, Massachusetts 02115.
Holmberg
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Krull
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D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000032:analysis; D007782:Lactoglobulins / Q000032:analysis; D010782:Photolysis; D011489:Protein Denaturation; D011506:Proteins / Q000032:analysis*; D012259:Ribonuclease, Pancreatic / Q000032:analysis; D012709:Serum Albumin / Q000032:analysis
D007328:Insulin; D007782:Lactoglobulins; D011506:Proteins; D012709:Serum Albumin; D012259:Ribonuclease, Pancreatic