Changes in polypeptide expression following Trypanosoma cruzi differentiation from trypomastigotes to amastigotes.
Changes in the dynamic of expression of polypeptides following the differentiation from infective trypomastigotes to multiplicative amastigote forms of Trypanosoma cruzi were mapped by two-dimensional gel electrophoresis and quantitatively analyzed by laser densitometry. Following the differentiation from trypomastigotes to amastigotes the expression of the polypeptides 212, 183, 176, 149, 50-55, 43, 39, 34 and 28 kDa is turned off in multiplicative amastigotes, whereas the expression of the polypeptides 80, 66 (p.Is. 6.75-7.50), 42 and 38 kDa is turned on. After complete differentiation from trypomastigotes to amastigotes the expression of the polypeptides 43, 42, 33, 32, 29 and 23 kDa is up-regulated in amastigotes, whereas the expression of the acidic polypeptides 66 (p.Is. 6.27-6.64), 45-48 and 41-43 kDa is down-regulated.
Ruiz-Ruano
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A
Division of Biomedical Sciences, Meharry Medical College, Nashville, Tennessee 37208.
Villalta
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F
Lima
M F
MF
D000818:Animals; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics; D015800:Protozoan Proteins / Q000096:biosynthesis* / Q000235:genetics; D014349:Trypanosoma cruzi / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014709:Vero Cells
D010455:Peptides; D015800:Protozoan Proteins