Different porphobilinogen-deaminase forms in wild and mutant strains of Saccharomyces cerevisiae. A possible correlation with its segregants behaviour.
1. Different porphobilinogen-deaminase (PBG-D) enzyme forms were found for D 27 and D 27/C6 (HEM R+) strains of Saccharomyces cerevisiae. 2. PBG-D was partially purified and chromatographed on Sephadex G-100 in either the presence or absence of a protease inhibitor. For D 27 only one active peak was observed while for D 27/C6 strain two active peaks were found. 3. A correlation between this differential behaviour and the presence of HEM R+ gene was looked for employing two segregants of one tetrad from D 27 and D 27/C6 mating.
Correa Garcia
S R
SR
Centro de Investigaciones sobre Porfirinas y Porfirias-CIPYP (CONICET, F.C.E. y N., UBA), Ciudad Universitaria, Buenos Aires, Argentine.
Rossetti
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MV
Batlle
A M
AM
D000642:Ammonia-Lyases / Q000235:genetics* / Q000378:metabolism; D002850:Chromatography, Gel; D007527:Isoenzymes / Q000235:genetics; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics
D007527:Isoenzymes; D000642:Ammonia-Lyases; C100132:porphobilinogenase