Fractionation and immunological characterization of allergens and allergoids of Prosopis juliflora pollen.
Allergoids of Prosopis juliflora pollen were prepared by formalinization of crude allergen and glycoprotein. Fractionation of crude allergen and allergoids on Sephadex G-100 resulted in separation of proteins of varying molecular size and a glycoprotein of 81 to 13 KD. Allergoids prepared from the glycoprotein fractionated into two proteins of approximately 200 KD and more than 200 KD. Crossed immunoelectrophoresis indicated 12 and gel diffusion test 3 precipitating antigens incrude allergen extract; by these tests allergoids depicted 8 and 3 precipitin bands, respectively. The precipitin analysis showed heterogeneity of allergenic determinants and also variation in cross-immunogenicity of the formalinized derivatives. The skin prick and radioallergosorbent tests depicted greater activity of fractionated crude allergens than the allergoids. The above tests suggest altered and concealed antigenic determinants as result of formalinization of P. juliflora pollen which, however, showed reduced allergenic activity relative to the native allergen.
Thakur
I S
IS
Department of Environmental Sciences, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, India.
Kamal
Mishra
S
S
D000485:Allergens / Q000276:immunology*; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis; D007194:India; D008970:Molecular Weight; D011058:Pollen / Q000276:immunology*; D012882:Skin Tests
D000485:Allergens