Mass measurement of inositol phosphates from the longitudinal muscle of the guinea-pig small intestine: a comparison with radiotracer studies.
Changes in the levels of inositol phosphate isomers during muscarinic stimulation of longitudinal smooth muscle fragments have been measured in terms of their mass and their radioactivity value. The mass has been determined by combining a popular h.p.l.c. separation method with desalting, dephosphorylation, and final measurement of inositol liberated using an inositol dehydrogenase--NADH-linked bioluminescence reaction. The h.p.l.c. elution profiles of radiolabelled inositol phosphates were compared with the h.p.l.c. elution profiles of their masses.
Prestwich
S A
SA
Department of Pharmacology and Clinical Pharmacology, St. George's Hospital Medical School, London, England.
Bolton
T B
TB
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D046508:Culture Techniques; D006168:Guinea Pigs; D007295:Inositol Phosphates / Q000378:metabolism*; D007421:Intestine, Small / Q000502:physiology; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000502:physiology*; D011976:Receptors, Muscarinic / Q000502:physiology*; D015290:Second Messenger Systems / Q000502:physiology*
D007295:Inositol Phosphates; D011976:Receptors, Muscarinic