The porcine PGD gene is preferentially lost from chromosome 6 in pig x rodent somatic cell hybrids.
The 6-phosphogluconate dehydrogenase (PGD) and glucose phosphate isomerase (GPI) genes are both located on chromosome 6 in the pig (Sus scrofa domestica). Nonetheless, the PGD gene was absent in a total of 17 GPI-positive cell lines found in three independently derived panels of pig x rodent somatic cell hybrids. In most of these cell lines we found an apparently normal pig chromosome 6 at cytogenetic analysis. These results suggest instability of the porcine PGD gene region in interspecies hybrid cells.
Thomsen
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PD
Department of Animal Science and Animal Health, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
Bosma
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AA
Kaufmann
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U
Harbitz
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I
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002875:Chromosomes; D006224:Cricetinae; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics*; D006822:Hybrid Cells; D007527:Isoenzymes / Q000235:genetics; D010734:Phosphogluconate Dehydrogenase / Q000235:genetics*; D013552:Swine / Q000235:genetics*
D007527:Isoenzymes; D010734:Phosphogluconate Dehydrogenase; D005956:Glucose-6-Phosphate Isomerase