Muramyl peptide-binding sites are located inside target cells.
Using flow cytometry and fluorescence polarization analysis, specific muramyl peptide-binding sites were shown to be located inside T-lymphocytes, macrophages and neuroblastoma cells, but not inside B-cells. No binding sites were found on the cell surface. The number of binding sites for each cell type was determined. Two types of binding sites were observed for myelomonocytic WEHI-3 cells with Kd values of 21 and 540 nM. Inhibition analysis demonstrated that for effective binding, an intact glycopeptide molecule and D-configuration of isoglutamine residue are important.
Sumaroka
M V
MV
Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.
Litvinov
I S
IS
Khaidukov
S V
SV
Golovina
T N
TN
Kamraz
M V
MV
Komal'eva
R L
RL
Andronova
T M
TM
Makarov
E A
EA
Nesmeyanov
V A
VA
Ivanov
V T
VT
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D001665:Binding Sites; D001667:Binding, Competitive; D002240:Carbohydrate Sequence; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D005456:Fluorescent Dyes; D006020:Glycopeptides / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D013601:T-Lymphocytes / Q000378:metabolism
D005456:Fluorescent Dyes; D006020:Glycopeptides; C072334:fluoresceinthiocarbamoyl-Lys-GlcNAc-1-4-MurNAc-Ala-isoglutamine