title: Effect of spectrin on structure properties of lipid bilayers formed from mixtures of phospholipids. Fluorescence and microcalorimetric studies.
abstract: Effect of spectrin from human erythrocytes on structure properties of lipid bilayers formed from a mixture of phosphatidylethanolamine/phosphatidylserine (PE/PS) and/or phosphatidylethanolamine/phosphatidylcholine (PE/PC) was studied with the use of fluorescence and microcalorimetric methods. Spectrin did not affect the order parameter of lipids in PE/PS vesicles. However, spectrin binding to liposomes did influence temperature, half-width and enthalpy of phase transitions in mixtures of dimyristoylphosphatidylethanolamine (DMPE) and dimyristoylphosphatidylcholine (DMPC), and this effect was dependent on DMPE to DMPC weight ratio. A change in miscibility of the components in the presence of spectrin was observed and it might be due to spectrin-PE interactions.
authors.affiliation: Department of Biophysics, Academy of Medicine, Wroclaw, Poland.
authors.forename: A B
authors.initials: AB
authors.lastname: Hendrich
authors.forename: K
authors.initials: K
authors.lastname: Michalak
authors.forename: M
authors.initials: M
authors.lastname: Bobrowska
authors.forename: A
authors.initials: A
authors.lastname: Kozubek
mesh_terms: D001665:Binding Sites; D055592:Biophysical Phenomena; D001703:Biophysics; D002152:Calorimetry, Differential Scanning; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004910:Erythrocyte Membrane / Q000737:chemistry; D005454:Fluorescence Polarization; D006801:Humans; D066298:In Vitro Techniques; D008051:Lipid Bilayers / Q000737:chemistry*; D015394:Molecular Structure; D010714:Phosphatidylethanolamines / Q000737:chemistry; D013049:Spectrin / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D010714:Phosphatidylethanolamines; D013049:Spectrin; D004134:Dimyristoylphosphatidylcholine; C018525:1,2-dimyristoylphosphatidylethanolamine