title: Platelet activating factor as a proinflammatory mediator in acetic-induced colitis in the rat.
abstract: Platelet activating factor (PAF) induces neutrophilia and produces a variety of gastrointestinal lesions. The role of PAF as a proinflammatory mediator was examined by measuring the production of PAF and the efficacy of the PAF antagonists WEB 2086 and Ro 24-0238 in acetic acid (HOAc)-induced colitis. PAF levels within the colonic mucosa, as measured by radioimmunoassay, increased from 259 +/- 119 ng/mg in control tissue, to 616 +/- 266 ng/mg in HOAc inflamed tissue. The accumulation of neutrophils within the mucosa was decreased by 53 +/- 10% by pretreatment with 3 mg/kg WEB 2086, and by 43 +/- 11% by 3 mg/kg Ro 24-0238. PAF-induced neutrophilia had no effect on the severity of HOAc-induced colitis, therefore, PAF my be involved in sustaining the chronic inflammation of colitis.
authors.affiliation: Department of Pharmacology, Hoffmann-La Roche Inc., Nutley, NJ 07110.
authors.forename: P C
authors.initials: PC
authors.lastname: Will
authors.forename: T K
authors.initials: TK
authors.lastname: Thomas
authors.forename: L
authors.initials: L
authors.lastname: Iverson
authors.forename: D
authors.initials: D
authors.lastname: Buckman
authors.forename: W
authors.initials: W
authors.lastname: Weis
authors.forename: C
authors.initials: C
authors.lastname: Wilson
authors.forename: A
authors.initials: A
authors.lastname: Srivastava
mesh_terms: D000085:Acetates; D000818:Animals; D001381:Azepines / Q000494:pharmacology; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism*; D007249:Inflammation / Q000378:metabolism; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology / Q000502:physiology*; D053284:Polyunsaturated Alkamides; D011725:Pyridines; D051381:Rats; D011919:Rats, Inbred Strains; D014230:Triazoles / Q000494:pharmacology
chemical_list: D000085:Acetates; D001381:Azepines; D010972:Platelet Activating Factor; D053284:Polyunsaturated Alkamides; D011725:Pyridines; D014230:Triazoles; C052518:WEB 2086; C073133:Ro 24-0238