Human recombinant macrophage colony-stimulating factor (M-CSF) increases Cl-esterase inhibitor (Cl-INH) synthesis by human monocytes.
The ability of macrophage colony-stimulating factor (M-CSF) to influence production of complement proteins by cultured human monocytes was studied. Three-day-old cultures of human monocytes were treated with 250-1000 U/ml recombinant human M-CSF (Cetus Corporation, Emeryville, CA). After 3 days the M-CSF containing medium was replaced by the same medium without M-CSF, and the cells were cultured for 3 more days. Samples taken at the removal of M-CSF and 3 days later were tested for the concentration of factor B and Cl-esterase inhibitor (Cl-INH) using ELISA methods, and C2 by using an immunohaemolytic assay. M-CSF induced a marked dose-dependent increase in the synthesis of Cl-INH, but did not significantly change Bf and C2 production. The findings suggest that M-CSF is able to influence selectively the complement protein-producing ability of cultured human monocytes.
Schmidt
B
B
Department of Immunopathology, National Institute of Hematology and Blood Transfusion, Budapest, Hungary.
Gyapay
G
G
Válay
M
M
Füst
G
G
D002478:Cells, Cultured; D003174:Complement C1 Inactivator Proteins / Q000096:biosynthesis*; D003175:Complement C2 / Q000096:biosynthesis; D011415:Complement Factor B / Q000096:biosynthesis; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology
D003174:Complement C1 Inactivator Proteins; D003175:Complement C2; D011994:Recombinant Proteins; D016173:Macrophage Colony-Stimulating Factor; D011415:Complement Factor B