title: The direct determination of protein concentration for proteins immobilized on polystyrene microspheres.
abstract: In this paper we show how the Lowry method, designed for the determination of proteins in solution, can be used for the determination of proteins immobilized on the surface of microspheres (for protein concentrations higher than 10 micrograms/ml). Measurements were made for human serum albumin (HSA) and for immunoglobulins [rabbit immunoglobulins--antibodies against human fibrinogen (IgGF) and against fragment D of human fibrinogen (IgGFgD)] immobilized on the surface of polystyrene microspheres.
authors.affiliation: Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Lodz.
authors.forename: T
authors.initials: T
authors.lastname: Basinska
authors.forename: S
authors.initials: S
authors.lastname: Slomkowski
mesh_terms: D000818:Animals; D002138:Calibration; D003124:Colorimetry; D005338:Fibrin Fibrinogen Degradation Products / Q000276:immunology; D005340:Fibrinogen / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D008567:Membranes, Artificial; D008863:Microspheres; D011137:Polystyrenes; D011506:Proteins / Q000737:chemistry*; D011817:Rabbits; D012709:Serum Albumin / Q000737:chemistry; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; D007074:Immunoglobulin G; D008567:Membranes, Artificial; D011137:Polystyrenes; D011506:Proteins; D012709:Serum Albumin; D012996:Solutions; D005340:Fibrinogen