Additive effect of oestradiol-17 beta and serum on synthesis of deoxyribonucleic acid in guinea-pig endometrial cells in culture.
Normal guinea-pig endometrial cells, grown in primary culture, were made quiescent by serum depletion. Quiescent cells cultured in the control medium (containing 1% fetal calf serum treated with dextran-coated charcoal, DCC-FCS) showed a steady and weak rate of [3H]thymidine incorporation, but the addition of 15% fetal calf serum (FCS) or 10% DCC-FCS to the control medium induced a significant increase of DNA synthesis, demonstrating the responsiveness of the quiescent cells to stimulation. A lower but significant increase in [3H]thymidine incorporation was elicited by epidermal growth factor (EGF, 100 ng/ml) or insulin (10 micrograms/ml) added to the basal medium. Oestradiol-17 beta added to the control medium at concentrations ranging from 10(-10) to 10(-5) mol/l not only failed to increase but even inhibited [3H]thymidine incorporation at the highest concentrations tested. An additive effect was noticed when quiescent cells were incubated with oestradiol-17 beta (10(-9) mol/l) in the presence of 10% DCC-FCS, but no synergistic effect occurred when 2 x 10(-9) mol oestradiol-17 beta/l was combined with either EGF (100 ng/ml) or insulin (10 micrograms/ml). Oestradiol-17 beta appears unable alone to stimulate DNA synthesis in normal endometrial cells, but requires factor(s) present in fetal calf serum.
Alkhalaf
M
M
Unité de Recherche de Biochimie Hormonale et des Régulations, INSERM U 198, Besançon, France.
Mahfoudi
A
A
Propper
A Y
AY
Adessi
G L
GL
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D004357:Drug Synergism; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D007328:Insulin / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism
D003470:Culture Media; D007328:Insulin; D004958:Estradiol; D004815:Epidermal Growth Factor; D004247:DNA; D013936:Thymidine