[Kinetic properties of rat heart cathepsin D under normal conditions, during emotional-pain stress and in the post-stress period].
Cathepsin D preparations have been isolated from the heart of healthy animals and stress-surviving rats by the method of affine chromatography with the hemoglobin-biogel-P300 sorbent. To analysis of the obtained data permits concluding that acute stress stimulates activation of the catalytic function of cathepsin D in the heart. But the period after the stress accompanied by the consecutive proteolysis rate reduction, that can be explained, probably, by a change in enzyme conformation. The concentration of Ca2+ (10(-6), 10(-5) M) and cAMP (10(-7), 10(-6) M) exert a regulating influence on the cathepsin D activity in the heart in acute stress period and after it.
Iakushev
V S
VS
Krisanova
N V
NV
D000818:Animals; D002402:Cathepsin D / Q000378:metabolism*; D007700:Kinetics; D009206:Myocardium / Q000201:enzymology*; D010146:Pain / Q000201:enzymology*; D011487:Protein Conformation; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013315:Stress, Psychological / Q000201:enzymology*; D013997:Time Factors
D002402:Cathepsin D
Kineticheskie svoĭstva katepsina D serdtsa krys v norme, pri émotsional'no-bolevom stresse i v poststressovyĭ period.