Genetic deficiency of complement component C8 in the rabbit: evidence of a translational defect in expression of the alpha-gamma subunit.
We examined the molecular basis for rabbit C8 alpha-gamma deficiency (C8D) using human C8 cDNA probes. Sequential probing of normal rabbit poly(A)+RNA revealed messages of 2.1, 1.9, and 0.8 kb for alpha, beta, and gamma, respectively. Corresponding analysis of C8D rabbit poly(A)+RNA identified messages for alpha and gamma of the same size and amounts as normal rabbits. Thus, C8 alpha-gamma deficiency in the rabbit appears to be the result of a translational rather than a transcriptional defect.
Komatsu
M
M
Department of Animal Breeding and Genetics, National Institute of Animal Industry, Ibaraki, Japan.
Yamamoto
K
K
Mikami
H
H
Sodetz
J M
JM
D000818:Animals; D015152:Blotting, Northern; D003185:Complement C8 / Q000172:deficiency / Q000235:genetics*; D015870:Gene Expression; D046911:Macromolecular Substances; D015335:Molecular Probes; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits
D003185:Complement C8; D046911:Macromolecular Substances; D015335:Molecular Probes; D012333:RNA, Messenger