title: Extracellular matrix gene expression by human endothelial and smooth muscle cells.
abstract: In this study, the expression of extracellular matrix genes by vascular cells from human iliac blood vessels was characterized on the mRNA steady-state level by slot blot and Northern transfer analyses, as well as by in situ hybridization. Endothelial cells were isolated from adult human iliac arteries and veins, as well as from umbilical veins; smooth muscle cells were isolated from adult human iliac arteries and inferior vena cava. The results show that confluent umbilical vein endothelial cells expressed the genes that encode types I, III, IV and VI collagens, as well as fibronectin and laminin. In contrast, the iliac endothelial cells expressed the genes for types IV and V collagens, fibronectin and laminin; mRNA transcripts for types I, III and VI collagens were not detectable. The smooth muscle cells from iliac arteries or inferior vena cava displayed gene expression for types I, III, IV, V and VI collagens, fibronectin and laminin. The results indicate major differences in gene expression for the various types of collagens by human iliac endothelial and smooth muscle cells. Furthermore, the fetal-derived umbilical endothelial cells displayed differential collagen gene expression from that of adult iliac endothelial cells.
authors.affiliation: Department of Pathology, Thomas Jefferson University, Philadelphia, PA.
authors.forename: E M
authors.initials: EM
authors.lastname: Tan
authors.forename: E
authors.initials: E
authors.lastname: Glassberg
authors.forename: D R
authors.initials: DR
authors.lastname: Olsen
authors.forename: J P
authors.initials: JP
authors.lastname: Noveral
authors.forename: G A
authors.initials: GA
authors.lastname: Unger
authors.forename: J
authors.initials: J
authors.lastname: Peltonen
authors.forename: M L
authors.initials: ML
authors.lastname: Chu
authors.forename: E
authors.initials: E
authors.lastname: Levine
authors.forename: S
authors.initials: S
authors.lastname: Sollberg
mesh_terms: D000293:Adolescent; D000328:Adult; D015152:Blotting, Northern; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis / Q000235:genetics; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000235:genetics*; D005353:Fibronectins / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007083:Iliac Artery / Q000166:cytology; D007084:Iliac Vein / Q000166:cytology; D007797:Laminin / Q000096:biosynthesis / Q000235:genetics; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D014682:Vena Cava, Inferior / Q000166:cytology
chemical_list: D015342:DNA Probes; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D012333:RNA, Messenger; D003094:Collagen; D004247:DNA