Metalloproteinases in the mineralized compartments of porcine dentine as detected by substrate-gel electrophoresis.
Several gelatinolytic activities were detected in the 4M guanidine HCl-soluble fraction extracted from demineralized porcine dentine matrix. These matrix proteinases were active in the range of pH 6-9, and the activities were enhanced by calcium ions. The enzymes also degraded some of the non-collagenous proteins coexisting in the extract. The results indicate that the changes in the composition of non-collagenous proteins during the development of dentine are related to proteolytic enzyme activity.
Fukae
M
M
Department of Biochemistry, School of Dental Medicine, Tsurumi University, Yokohama, Japan.
Kaneko
I
I
Tanabe
T
T
Shimizu
M
M
D000418:Albumins / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D002118:Calcium / Q000494:pharmacology; D003804:Dentin / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008666:Metalloendopeptidases / Q000032:analysis / Q000302:isolation & purification*; D008903:Minerals / Q000032:analysis; D008970:Molecular Weight; D015676:Osteonectin / Q000032:analysis; D010750:Phosphoproteins / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate; D013552:Swine; D014083:Tooth Germ / Q000737:chemistry*; D060749:alpha-2-HS-Glycoprotein
D000418:Albumins; D000596:Amino Acids; D001798:Blood Proteins; D008903:Minerals; D015676:Osteonectin; D010750:Phosphoproteins; D060749:alpha-2-HS-Glycoprotein; C017630:phosphophoryn; D012967:Sodium Dodecyl Sulfate; D008666:Metalloendopeptidases; D002118:Calcium