title: [Chromatographic analysis and immunobiologic properties of tuberculins].
abstract: Immunobiologic activities of tuberculin preparations and their components have been comparatively studied using gel filtration and high-performance liquid chromatography (HPLC) techniques. It is shown that high-molecular weight fraction of protein-purified derivate tuberculin (PPD) had higher activity as compared to nonfractionated preparation in skin tests on guinea pigs sensitized with Mycobacterium bovis BCG as well as in enzyme-linked immunosorbent assay with affinity purified rabbit antibodies against PPD. Using the preparative HPLC-technique we failed to isolate a component of PPD having greater tuberculin test potency than nonfractionated preparation.
authors.forename: S V
authors.initials: SV
authors.lastname: Komissarenko
authors.forename: S A
authors.initials: SA
authors.lastname: Bobrovnik
authors.forename: K P
authors.initials: KP
authors.lastname: Liashchenko
authors.forename: A V
authors.initials: AV
authors.lastname: Nartikov
authors.forename: Iu I
authors.initials: IuI
authors.lastname: Petrova
authors.forename: E V
authors.initials: EV
authors.lastname: Gorbenko
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D008970:Molecular Weight; D009163:Mycobacterium bovis / Q000276:immunology; D014373:Tuberculin / Q000032:analysis* / Q000276:immunology; D014374:Tuberculin Test
chemical_list: D014373:Tuberculin
vernacular_title: Khromatograficheskiĭ analiz i immunobiologicheskie svoĭstva tuberkulinov.