title: Evaluation of edetate and thiamine for treatment of experimentally induced environmental lead poisoning in cattle.
abstract: Twenty mature Holstein cows were randomized into 5 treatment groups. Cows of groups 2 to 5 were given 2 mg of elemental Pb/kg of body weight for 28 days. Clinical signs of plumbism were scored, and blood for Pb, progesterone, and hematologic analyses was collected weekly. Cows also were examined weekly for anomalous ovarian cycles. Starting on study day 28, cows in group 3 were treated once daily with 2 mg of thiamine HCl/kg (IM) for 13 days, cows in group 4 were treated twice daily with 62 mg of Na2,Ca-EDTA/kg (IV) for 4 days, and cows in group 5 were given thiamine (dosage regimen the same as for group 3) plus Na2,Ca-EDTA (dosage regimen the same as for group 4). On study days 96 through 139, cows were slaughtered in a commercial abattoir and samples of blood, skeletal muscles, bones, liver, and kidneys were collected and assayed for Pb concentration. Thiamine was not effective in reducing blood Pb concentration, and treatment with Na2,Ca-EDTA and thiamine plus Na2,Ca-EDTA was effective in reducing the concentration of Pb in blood. However, treatment with thiamine was more effective than treatment with Na2,Ca-EDTA or thiamine plus Na2,Ca-EDTA in inducing remission of clinical signs of plumbism. The concentration of Pb in blood was significantly (P less than 0.05) correlated to the concentration of Pb in liver, kidneys, skeletal muscles, and bones. Significant (P less than 0.05) relationship existed between number of days from Pb exposure to slaughter and concentration of Pb in blood, liver, and skeletal muscles. Exposure to Pb did not significantly alter CBC values.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Veterinary Biosciences, College of Veterinary Medicine, University of Illinois, Urbana 61801.
authors.forename: R W
authors.initials: RW
authors.lastname: Coppock
authors.forename: W C
authors.initials: WC
authors.lastname: Wagner
authors.forename: J D
authors.initials: JD
authors.lastname: Reynolds
authors.forename: R S
authors.initials: RS
authors.lastname: Vogel
authors.forename: H B
authors.initials: HB
authors.lastname: Gelberg
authors.forename: L Z
authors.initials: LZ
authors.lastname: Florence
authors.forename: W A
authors.initials: WA
authors.lastname: Wolff
mesh_terms: D000818:Animals; D001706:Biopsy / Q000662:veterinary; D001842:Bone and Bones / Q000737:chemistry; D002417:Cattle; D002418:Cattle Diseases / Q000139:chemically induced / Q000188:drug therapy*; D004492:Edetic Acid / Q000627:therapeutic use*; D005260:Female; D007668:Kidney / Q000737:chemistry; D007854:Lead / Q000032:analysis / Q000097:blood; D007855:Lead Poisoning / Q000188:drug therapy / Q000662:veterinary*; D008099:Liver / Q000737:chemistry; D009132:Muscles / Q000737:chemistry; D011897:Random Allocation; D013831:Thiamine / Q000627:therapeutic use*
chemical_list: D007854:Lead; D004492:Edetic Acid; D013831:Thiamine