An apparatus for recording synaptic potentials from neuronal cultures using voltage-sensitive fluorescent dyes.
Voltage-sensitive dyes offer the promise of noninvasive multicell recording of electrical activity, and should therefore be useful for studying the synaptic interactions of small networks of cultured neurons. We have designed and built a system for recording from microcultures of 1-15 neurons from the rat superior cervical ganglion (SCG), using voltage-sensitive fluorescent dyes of the styryl class. The apparatus comprises a standard inverted epifluorescence microscope; a mercury arc lamp with an optical feedback regulator; a 256-pixel fiber-optic camera with individual photodiode detectors and very low-noise amplifiers; and a personal computer-based data acquisition system. Its dark noise and illumination fluctuations are low enough that at typical fluorescence levels for these cells, it is limited by shot noise (the inherent physical limit of detection). Recording from SCG neurons, the signal-to-noise ratio is high enough to see large subthreshold synaptic potentials without signal averaging. This apparatus should be useful for studying long-term synaptic plasticity in cultures of vertebrate neurons, and several of its features should apply to optical recording from other preparations.
Chien
C B
CB
Department of Physics, California Institute of Technology, Pasadena 91125.
Pine
J
J
D000703:Analog-Digital Conversion; D000818:Animals; D002478:Cells, Cultured; D003201:Computers; D004581:Electronics; D004594:Electrophysiology / Q000295:instrumentation; D004867:Equipment Design; D005336:Fiber Optic Technology; D005456:Fluorescent Dyes; D005728:Ganglia, Sympathetic / Q000166:cytology; D008838:Microcomputers; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D009415:Nerve Net / Q000502:physiology; D009482:Neurophysiology / Q000295:instrumentation*; D055100:Optical Fibers; D011726:Pyridinium Compounds; D051381:Rats; D012984:Software; D013343:Styrenes; D013569:Synapses / Q000502:physiology; D014159:Transducers; D014743:Videotape Recording / Q000295:instrumentation*
D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; C072935:RH 423; D013343:Styrenes