[Preparation of immunoenzyme conjugates of beta-lactamase from Bacillus licheniformis 749/c and horseradish peroxidase with human antibodies to HIV-1].
By using three different linkage methods with carbodiimide, glutaraldehyde and periodite, immunoenzyme conjugates of beta-lactamase from Bacillus licheniformis 749/c and horse radish peroxidase with human antibodies to HIV-1 were prepared. The human antibodies were purified by the affinity procedure on Protein-A-Sepharose 6B. The conjugates were tested in a solid phase immunoenzymatic system for the HIV-1 antigen. It was shown that the conjugates prepared by the carbodiimide linkage method had the highest titer, the beta-lactamase conjugate being superior by its titer to the respective peroxidase conjugate. In the lyophilized state the conjugates prepared with the carbodiimide linkage method were stable.
Sazykin
A Iu
AIu
Eremin
S A
SA
Egorov
A M
AM
Starov
A I
AI
Sakaian
I A
IA
Matsevich
G R
GR
Gibadulin
R A
RA
D001407:Bacillus / Q000201:enzymology*; D015483:HIV Antibodies / Q000276:immunology*; D006735:Horseradish Peroxidase / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D001618:beta-Lactamases / Q000276:immunology*
D015483:HIV Antibodies; D006735:Horseradish Peroxidase; D001618:beta-Lactamases
Poluchenie immunofermentnykh kon"iugatov beta-laktamazy iz Bacillus licheniformis 749/c i peroksidazy khrena s chelovecheskimi antitelami k VICh-1.