title: Unique DNA plasmid pRS64 associated with chromosomal DNAs of the plant pathogenic fungus Rhizoctonia solani.
abstract: Unique DNA sequences homologous to the linear DNA plasmid pRS64 were investigated in chromosomal DNAs of isolates belonging to anastomosis group 4 (AG-4) of the plant pathogenic fungus Rhizoctonia solani. Chromosome-sized DNAs of isolates RI-64 and 1271 of AG-4 were separated into six bands by orthogonal-field-alternation gel electrophoresis and hybridized to a cloned segment of pRS64. A small chromosome-sized DNA band of approximately 1.1 Mb carried the sequences homologous to pRS64 DNA. Sequences homologous to pRS64 were also maintained within the chromosomal DNA of isolate 127.1 of AG-4 which does not possess the plasmid. The plasmid showed no homology to the mitochondrial DNA of isolate 1271. The possibility that the linear plasmid pRS64 may act as a transposable genetic element is discussed.
authors.affiliation: Faculty of Agriculture, Tohoku University, Sendai, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Wako
authors.forename: T
authors.initials: T
authors.lastname: Ishikawa
authors.forename: T
authors.initials: T
authors.lastname: Hashiba
mesh_terms: D015139:Blotting, Southern; D002498:Centrifugation; D015825:Chromosomes, Fungal; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D004587:Electrophoresis, Agar Gel; D010957:Plasmids; D012232:Rhizoctonia / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial