Monoclonal antibodies against human plasma protein C and their uses for immunoaffinity chromatography.
Human protein C, isolated by conventional multistep methods, was used for immunization of mice. Monoclonal antibodies were prepared and screening of antibodies to human protein C was achieved using an immunoblotting technique. Five monoclonal anti-protein C antibodies were compared as affinity ligands. Different parameters were studied (adsorption capacity, specificity of adsorption, possibility of desorption under mild conditions) and two antibodies were selected. One antibody allows preparation of highly purified protein C in a single-step procedure from a fraction of plasma containing high levels of coagulation factors whereas the other can be used for preparation of protein C deficient plasma.
Regnault
V
V
INSERM U 284, Plateau de Brabois, CO 10, Vandoeuvre-lès-Nancy, France.
Rivat
C
C
Pfister
M
M
Stoltz
J F
JF
D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D007163:Immunosorbent Techniques; D011486:Protein C / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D011486:Protein C