The relationship between red cell aging and enzyme activities in experimental animals.
1. The relationship between red cell aging and enzyme activities was studied in rabbit, guinea-pig, hamster, rats (F344/N and SD), and mice (BALB/c and DBA/2). 2. The activities of six enzymes: glucose-6-phosphate dehydrogenase (G-6-PD), 6-phosphogluconate dehydrogenase (6-PGD), hexokinase (Hx), glutamate oxaloacetate transminase (GOT), lactate dehydrogenase (LDH) and acetylcholinesterase (AChE), were measured in the red cells of different ages which were obtained either by centrifugation or experimental anaemia. 3. Hx, AChE and GOT activities were much higher in younger red cells than in older cells, hence the activities of these enzymes may be used as an indicator of age of the cells.
Shimizu
Y
Y
Department of Veterinary Medicine, Faculty of Agriculture, Gifu University, Japan.
Suzuki
M
M
D000110:Acetylcholinesterase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D006224:Cricetinae; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000201:enzymology* / Q000502:physiology; D005954:Glucosephosphate Dehydrogenase / Q000097:blood; D006168:Guinea Pigs; D006593:Hexokinase / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008647:Mesocricetus; D051379:Mice; D008815:Mice, Inbred Strains; D010734:Phosphogluconate Dehydrogenase / Q000097:blood; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D007770:L-Lactate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D001219:Aspartate Aminotransferases; D006593:Hexokinase; D000110:Acetylcholinesterase