Kinetics of a rapid, luminol dependent chemiluminescence signal induced in HL-60 cells by amphotericin B and other stimulants.
Addition of the polyene antibiotic amphotericin B or tissue culture medium to nondifferentiated HL-60 cells in the presence of luminol induces a chemiluminescence signal that reaches a peak value within a few seconds and decays exponentially in less than a minute. The kinetics of the signal and its modulation by superoxide dismutase, catalase, and horseradish peroxidase are consistent with a series of solution biochemical processes with a rate-determining step corresponding to the disproportionation of a luminol-superoxide complex. The effects of the enzymes demonstrate that superoxide is a precursor to the rate-determining intermediate and that both catalase and peroxide enhance a reaction that competes with the rate-limiting process.
O'Keefe
D A
DA
Department of Chemistry, University of Western Ontario, London, Canada.
James
D R
DR
Ware
W R
WR
Petersen
N O
NO
D000666:Amphotericin B / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D006735:Horseradish Peroxidase / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol / Q000378:metabolism; D013482:Superoxide Dismutase / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D008165:Luminol; D000666:Amphotericin B; D006735:Horseradish Peroxidase; D002374:Catalase; D013482:Superoxide Dismutase