Cloning of R kappa B, a novel DNA-binding protein that recognizes the interleukin-2 receptor alpha chain kappa B site.
Transcriptional activation of the interleukin-2 receptor alpha (IL-2R alpha) gene in T cells is dependent on a regulatory element that can bind NF-kappa B but differs in sequence and function from the kappa B site of the immunoglobulin (Ig) enhancer. To define the molecular basis of gene-specific regulation by this variant kappa B site, we have used electrophoretic mobility shift assays to characterize a novel gene product, designated R kappa B, that binds preferentially to the related kappa B site in IL-2R alpha. A cDNA encoding this 107-kD protein has been isolated from a lambda gt11 expression library by screening with a probe containing the IL-2R alpha kappa B site. R kappa B is a tissue-specific transcription factor that contains an amino acid sequence similar to a discrete region of the myogenic regulatory protein MyoD, but is unrelated to other DNA-binding proteins, including those belonging to the rel/dorsal gene family. This novel kappa B binding protein may therefore contribute to the regulation of distinct cellular and viral genes with variant kappa B sites.
Adams
B S
BS
Howard Hughes Medical Institute, University of Michigan Medical Center, Department of Internal Medicine, Ann Arbor 48109-0650.
Leung
K Y
KY
Hanley
E W
EW
Nabel
G J
GJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D002467:Cell Nucleus / Q000502:physiology; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004742:Enhancer Elements, Genetic / Q000187:drug effects; D015723:Gene Library; D016356:Gene Products, tax / Q000235:genetics / Q000378:metabolism; D005803:Genes, Immunoglobulin; D006801:Humans; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D015375:Receptors, Interleukin-2 / Q000235:genetics*; D011994:Recombinant Proteins / Q000494:pharmacology; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D004268:DNA-Binding Proteins; D016356:Gene Products, tax; D046911:Macromolecular Substances; C072725:NFRKB protein, human; D009838:Oligodeoxyribonucleotides; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D013755:Tetradecanoylphorbol Acetate