[Effects of anti-cholinoceptor antibodies on the frog heart].
Research on isolated hearts of Rana temporaria has shown that upon treating them with anti-cholinoreceptor antibodies, there occurs a considerable reduction of inhibition effect on cardiac activity of acetylcholine or carbacholine. A reduction of inhibition effect was noticed after incubation of frog's heart with antibodies against cholinoreceptors obtained from motor-denervated muscles of frogs as well as from muscles of mice Balb/c. Cholinoreceptor protein was obtained and purified by A. Sobel's method. Control tests were made with serum of non-immunized rabbits and rabbits immunized with material obtained from non-denervated muscles of frogs. It was concluded that acetylcholinoreceptor antibodies are capable of provoking atropine-like effect on frog's heart. According to our data, anti-cholinoreceptor antibodies as well as cholinoreceptors are relatively non-specific to species.
Burlakov
G V
GV
Kamysheva
V A
VA
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002217:Carbachol / Q000494:pharmacology; D005260:Female; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009200:Myocardial Contraction; D009206:Myocardium / Q000276:immunology*; D011817:Rabbits; D011896:Rana temporaria; D011950:Receptors, Cholinergic / Q000276:immunology*
D000906:Antibodies; D011950:Receptors, Cholinergic; D002217:Carbachol; D000109:Acetylcholine
O deĭstvii antikholinoretseptornykh antitel na serdtse liagushki.