Application of bromodeoxyuridine immunohistochemistry to the rat temporomandibular joint.
To detect S-phase cells in the decalcified paraffin-embedded temporomandibular joint (TMJ) tissues of the rat, bromodeoxyuridine (BrdUrd) immunohistochemistry was performed. Percardiac perfusion of 10% EDTA-2Na Tris buffer accelerated the decalcification. The use of the monoclonal antibody to BrdUrd enabled the visualization of the distribution of S-phase cells. This method is practical in studying cell kinetics and the specific proliferation mode of the TMJ.
Sekine
J
J
Second Department of Oral and Maxillofacial Surgery, Nagasaki University, School of Dentistry, Japan.
Sano
K
K
Hironaka
R
R
Inokuchi
T
T
D000818:Animals; D001854:Bone Marrow Cells; D001973:Bromodeoxyuridine; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D016196:S Phase; D013704:Temporomandibular Joint / Q000737:chemistry / Q000166:cytology*
D001973:Bromodeoxyuridine