Renal pelvic carcinoma which shows metastatic potential to distant organs, induced by N-butyl-N-(4-hydroxybutyl)nitrosamine in NON/Shi mice.
Renal pelvic carcinoma was induced in mice by giving N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN). Initially, differences in renal pelvic carcinogenesis by BBN were examined in three male mouse strains: NON/Shi, which demonstrate spontaneous hydronephrosis with incidences of 10-30%, and DS/Shi and B6C3F1, which do not exhibit hydronephrosis. When mice of these strains were given 0.05% BBN in the drinking water for 12 weeks followed by water without BBN for 8 weeks, renal pelvic carcinoma morphologically similar to human carcinomas developed in 8 of 23 NON/Shi mice (35%). Metastasis to the lung was found in one of them (13%). B6C3F1 and DS/Shi mice had no pelvic tumors, but the response to urinary bladder carcinogenesis in NON/Shi mice was nearly equal to that in DS/Shi and B6C3F1 mice. These results suggest that renal pelvic carcinogenesis is related to the presence of stagnant urine containing carcinogen in the renal pelvis. In a second experiment, we examined renal pelvic carcinogenesis in NON/Shi mice given BBN for 4 weeks followed by water without BBN for 32 weeks. The incidence of renal pelvic carcinoma (28%) was similar to that in the first experiment, but the incidence of metastasis was markedly elevated to 60%. These results indicate that BBN treatment can induce renal pelvic carcinoma which often metastasizes to the lung in NON/Shi mice.
Murai
T
T
Aburahi Laboratories, Shionogi Research Laboratories, Shionogi Co., Ltd., Shiga.
Mori
S
S
Hosono
M
M
Takeuchi
Y
Y
Ohara
T
T
Makino
S
S
Takeda
R
R
Hayashi
Y
Y
Fukushima
S
S
D000008:Abdominal Neoplasms / Q000556:secondary; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002085:Butylhydroxybutylnitrosamine; D004195:Disease Models, Animal; D006869:Hydronephrosis / Q000139:chemically induced; D007680:Kidney Neoplasms / Q000139:chemically induced* / Q000473:pathology; D007682:Kidney Pelvis / Q000187:drug effects* / Q000473:pathology; D008175:Lung Neoplasms / Q000556:secondary; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009362:Neoplasm Metastasis / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced
D002085:Butylhydroxybutylnitrosamine