[Cytoplasmic maturation of cow oocytes cultured in vitro and their dehydrogenase activity].
Approximately in 80% of cow oocytes (n = 632) ended cytoplasmatic and nucleus maturation to the state of metaphase II in the conditions of 24 hours in vitro cultivation. In 300 oocytes cytochemically we have determined the activity of enzymes--the succinate dehydrogenase (SDH, EC 1.3.99.1.), alpha-glycerophosphate dehydrogenase (GPDH, EC 1.1.1.8.) and glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49.). The reaction intensity of the observed dehydrogenases increased in cow oocytes which were cultivated in vitro for 24 hours. Dehydrogenases are located in the mitochondria which are laid out regularly in the cytoplasm of oocytes. Part of oocytes showed polarization in the lay out of reaction and part of oocytes gave extramitochondrial reaction.
Pivko
Iu
Iu
Laurinchuk
I
I
Bukarov
N
N
Grafenau
P
P
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D003593:Cytoplasm / Q000648:ultrastructure*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D005993:Glycerolphosphate Dehydrogenase / Q000032:analysis; D008961:Models, Structural; D009865:Oocytes / Q000201:enzymology / Q000254:growth & development*; D010088:Oxidoreductases / Q000032:analysis*; D013385:Succinate Dehydrogenase / Q000032:analysis; D013997:Time Factors
D010088:Oxidoreductases; D005993:Glycerolphosphate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase
Tsitoplazmaticheskoe sozrevanie kul'tiviruemykh vne organizma ootsitov korov i ikh degidrogenaznaia aktivnost'.