title: Micro-determination of L-gulono-gamma-lactone oxidase activity.
abstract: Highly sensitive assay method of L-gulono-gamma-lactone oxidase (GLO) was constructed. In this method, L-ascorbic acid formed in the enzymatic reaction was converted to its bis(dinitrophenyl)hydrazone derivative, and the amount of the latter was determined by high-performance liquid chromatography. Twenty picomoles of ascorbic acid was detected, which makes this method 25 times more sensitive than the previously used dipyridyl one. By the present method, a minute activity of GLO in liver microsomes prepared from rats of the Osteogenic Disorder Shionogi strain (ODS-od/od) could be measured.
authors.affiliation: Institute of Applied Biochemistry, Mitake, Gifu, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kito
authors.forename: N
authors.initials: N
authors.lastname: Ohishi
authors.forename: K
authors.initials: K
authors.lastname: Yagi
mesh_terms: D000818:Animals; D001205:Ascorbic Acid; D001848:Bone Diseases, Developmental / Q000201:enzymology / Q000235:genetics; D007700:Kinetics; D050544:L-Gulonolactone Oxidase; D008832:Microchemistry; D008862:Microsomes, Liver / Q000201:enzymology; D051381:Rats; D011922:Rats, Mutant Strains; D013401:Sugar Alcohol Dehydrogenases / Q000032:analysis* / Q000378:metabolism
chemical_list: D013401:Sugar Alcohol Dehydrogenases; D050544:L-Gulonolactone Oxidase; D001205:Ascorbic Acid