Rapid detection of type A influenza viruses with monoclonal antibodies to the M protein (M1) by enzyme-linked immunosorbent assay and time-resolved fluoroimmunoassay.
Monoclonal antibodies (MAbs) to the M protein (M1) were used in the development of direct detection systems for type A influenza viruses in clinical specimens. Optimal detection by an enzyme-linked immunosorbent assay was achieved when MAbs were used as capture antibodies and rabbit polyclonal antibodies were used as sandwich antibodies. Detection by the enzyme-linked immunosorbent assay required amplification of the virus. direct detection in clinical specimens (nasopharyngeal aspirates) was accomplished when MAbs recognizing two distinct antigenic sites of M1 were used in a time-resolved fluoroimmunoassay. Type A influenza viruses could be detected equally well in specimens obtained during epidemics of both H3N2 and H1N1 influenza viruses.
Bucher
D J
DJ
Department of Microbiology and Immunology, New York Medical College, Valhalla 10595.
Mikhail
A
A
Popple
S
S
Graves
P
P
Meiklejohn
G
G
Hodes
D S
DS
Johansson
K
K
Halonen
P E
PE
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D004797:Enzyme-Linked Immunosorbent Assay; D015200:Fluoroimmunoassay; D006801:Humans; D009980:Influenza A virus / Q000276:immunology* / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis / Q000382:microbiology; D014763:Viral Matrix Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; C031540:M-protein, influenza virus; C488936:M1 protein, Influenza A virus; D014763:Viral Matrix Proteins