title: Micro-liquid chromatography method for the determination of ciclopiroxolamine after pre-column derivatization in topical formulations.
abstract: Ciclopiroxolamine is a broad-spectrum antimycotic drug. Neither the free acid (ciclopirox) nor its salt with ethanolamine (ciclopiroxolamine) can directly be quantified by liquid chromatography (LC) on both normal and reversed phases. This is due to the chelating function of the N-hydroxypyridone group that interacts strongly with stationary phases. Derivatization by alkylation forms a 1-alkyloxypirydone with regular chromatographic behaviour. A micro-LC method based on an isocratic elution reversed-phase system for quantification of ciclopiroxolamine in topical formulations is described. Chromatography was carried out using an LC Packings fused-silica capillary column (15 cm x 330 microns I.D.; Delta Pak, RP-18, 5 microns, 300 A) coupled to a Kontron 433 UV capillary detector. Data with respect to the derivatization reaction, recovery, reproducibility and limits of detection of the proposed method are reported and discussed.
authors.affiliation: Dipartimento di Scienza e Tecnologia del Farmaco, Facoltà di Farmacia, Università di Torino, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Belliardo
authors.forename: A
authors.initials: A
authors.lastname: Bertolino
authors.forename: G
authors.initials: G
authors.lastname: Brandolo
authors.forename: C
authors.initials: C
authors.lastname: Lucarelli
mesh_terms: D000287:Administration, Topical; D000935:Antifungal Agents / Q000008:administration & dosage / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D000077768:Ciclopirox; D011728:Pyridones / Q000008:administration & dosage / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000935:Antifungal Agents; D011728:Pyridones; D000077768:Ciclopirox