Monitoring anthracycline binding to nucleotides by two dimensional cosy NMR experiments.
The nucleotide binding ability of the novel anthracycline drug, 3-fluoro-4-demethoxydaunomycin, has been studied by two dimensional 1H NMR correlated spectroscopy (COSY). In the COSY spectrum of the nucleotide mini-helix d(CTGCAG)2 cross-peaks are observed from the spin-coupled H6 and H5 protons of the cytidine bases. Additional cytidine H6/H5 cross-peaks are observed in the COSY spectrum of the anthracycline-d(CTGCAG)2 complex. These additional cytidine cross-peaks enable the identification of the anthracycline binding sites and the determination of the relative kinetic stability of the bound drug at each binding site.
Hammer
B C
BC
Department of Chemistry, University College, University of New South Wales, Australian Defence Force Academy, Canberra.
Russell
R A
RA
Warrener
R N
RN
Collins
J G
JG
D001665:Binding Sites; D003562:Cytidine / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D003630:Daunorubicin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D007364:Intercalating Agents / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*
D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides; C065327:3-fluoro-4-demethoxydaunomycin; D003562:Cytidine; D004247:DNA; D003630:Daunorubicin