title: Lack of HIV-1 V3 region sequence diversity in two haemophiliac patients infected with a putative biologic clone of HIV-1.
abstract: Peripheral blood mononuclear cells (PBMC) from two haemophilia B patients, who presumably became infected with a putative HIV-1 biological clone following treatment with the same suspected batch of commercial factor, were used to clone and sequence the hypervariable V3 region of the HIV-1 envelope protein. In 10 of 12 clones the V3 region was identical and two (one from each patient) had a single non-synonymous point mutation. Viable reisolates (shown to be authentic by sequencing of V3) currently appear to be monocyte tropic. These results strongly indicate that the patients were infected from a common source with a very low number of infectious particles and indicate that variability under these conditions is limited.
authors.affiliation: Paul-Ehrlich-Institut, Langen, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Cichutek
authors.forename: S
authors.initials: S
authors.lastname: Norley
authors.forename: R
authors.initials: R
authors.lastname: Linde
authors.forename: W
authors.initials: W
authors.lastname: Kreuz
authors.forename: M
authors.initials: M
authors.lastname: Gahr
authors.forename: J
authors.initials: J
authors.lastname: Löwer
authors.forename: G
authors.initials: G
authors.lastname: von Wangenheim
authors.forename: R
authors.initials: R
authors.lastname: Kurth
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001769:Blood / Q000382:microbiology; D002675:Child, Preschool; D005164:Factor IX / Q000009:adverse effects; D014644:Genetic Variation; D015699:HIV Envelope Protein gp120 / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006467:Hemophilia A / Q000150:complications; D006801:Humans; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
chemical_list: D015699:HIV Envelope Protein gp120; D005164:Factor IX