The ade6 gene of the fission yeast Schizosaccharomyces pombe has the same chromatin structure in the chromosome and in plasmids.
We have analysed the chromatin structure of the ade6 gene of Schizosaccharomyces pombe and its flanking regions both in the chromosome and in plasmids. The chromatin structure is independent of the chromosomal or extrachromosomal location. The ade6 gene contains eight precisely positioned nucleosomes on the 5' half, 'not positioned' nucleosomes around the 3' end and a nuclease-sensitive promoter region. Precisely positioned nucleosomes, but no nuclease-sensitive region were also detected on the ura4 gene in the chromosome and on a plasmid. The results show that S. pombe chromosomal and extrachromosomal genes have chromatin structures similar to those of S. cerevisiae and higher eukaryotes.
Bernardi
F
F
Institut für Zellbiologie, ETH-Hönggerberg, Zürich, Switzerland.
Koller
T
T
Thoma
F
F
D002843:Chromatin / Q000648:ultrastructure*; D015825:Chromosomes, Fungal / Q000648:ultrastructure*; D004271:DNA, Fungal / Q000032:analysis; D005800:Genes, Fungal; D010957:Plasmids; D015183:Restriction Mapping; D012568:Schizosaccharomyces / Q000235:genetics* / Q000648:ultrastructure
D002843:Chromatin; D004271:DNA, Fungal