Secretion of oligomeric Val8-human calcitonin by Saccharomyces cerevisiae.
Monomeric human calcitonin (hCT) gene and oligomeric hCT genes composed of two, three or four head-to-tail linked monomers were fused in-frame to the yeast alpha-factor leader coding sequence wild-type and fragile mutant Saccharomyces cerevisiae strains were transformed with the constructed plasmids and the yield of recombinant protein secreted into the culture medium was measured. The yeast cells secreted equal (molar) amounts of all of the hCT variants. The recombinant proteins remained stable in the growth medium for at least 3 days. The fragile cells secreted about 30% more hCT as compared to the wild-type yeast cells.
Mironova
R
R
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Philipova
D
D
Marekov
L
L
Venkov
P
P
Ivanov
I
I
D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D002116:Calcitonin / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D046911:Macromolecular Substances; D011994:Recombinant Proteins; C050937:calcitonin, human, Val(8)-; D002116:Calcitonin