title: [The preparation of highly purified insulin and its clinical use. Insulin Cooperative Study Group].
abstract: Antigenicity of insulin preparations is obviously related to their purity. The average contents of proinsulin, glucagon and pancreatic polypeptide in highly purified insulin (HP-I) produced by us are 76.0, 2.11 and 0.11 ppm respectively. These results are superior to those for domestic conventional crystalline insulin and single peak insulin (SP-I). The antibodies to insulin, proinsulin and pancreatic polypeptide in serum were examined in 24 diabetic patients treated with HP-I for more than 6 months. The positive rates for the above three antibodies were 37.5%, 0% and 0% respectively. The results showed that the antigenicity of HP-I was much lower than that of domestic conventional crystalline insulin and almost the same as that of MC-I from Nordisk, Denmark.
authors.affiliation: Department of Biochemistry, School of Pharmacy.
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: F
authors.initials: F
authors.lastname: Fang
authors.forename: X
authors.initials: X
authors.lastname: Zhu
authors.forename: S
authors.initials: S
authors.lastname: Deng
authors.forename: W
authors.initials: W
authors.lastname: Zhou
authors.forename: Z
authors.initials: Z
authors.lastname: Xing
mesh_terms: D001323:Autoantibodies / Q000097:blood; D003920:Diabetes Mellitus / Q000097:blood / Q000188:drug therapy*; D006801:Humans; D007328:Insulin / Q000276:immunology / Q000302:isolation & purification* / Q000627:therapeutic use; D010191:Pancreatic Polypeptide / Q000276:immunology; D011384:Proinsulin / Q000276:immunology
chemical_list: D001323:Autoantibodies; D007328:Insulin; D010191:Pancreatic Polypeptide; D011384:Proinsulin