Green tea extract inhibits nucleoside transport and potentiates the antitumor effect of antimetabolites.
The present study provides evidence that green tea extract (GTE), consisting of polyphenol components, is a highly active nucleoside transport inhibitor. GTE markedly inhibited radiolabeled thymidine and uridine transport in mouse leukemia L1210 cells, with IC50 values of 3.2 and 8.0 mumol/L, respectively. GTE blocked the rescue effect of exogenous nucleosides and enhanced the cytotoxicity of AraC and MTX to L1210 cells and human hepatoma BEL-7402 cells. GTE markedly potentiated the inhibitory effect of AraC on leukemia L1210 and P388 in mice. These results indicate that GTE is potentially useful when combined with antimetabolites in cancer chemotherapy.
Zhen
Y
Y
Institute of Medical Biotechnology, CAMS, Beijing.
Cao
S
S
Xue
Y
Y
Wu
S
S
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic; D003561:Cytarabine / Q000494:pharmacology*; D004357:Drug Synergism; D005260:Female; D006801:Humans; D007939:Leukemia L1210 / Q000473:pathology; D007941:Leukemia P388 / Q000473:pathology; D008297:Male; D008727:Methotrexate / Q000494:pharmacology*; D051379:Mice; D008811:Mice, Inbred DBA; D010936:Plant Extracts / Q000494:pharmacology*; D013662:Tea; D013936:Thymidine / Q000493:pharmacokinetics; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014410:Tumor Stem Cell Assay; D014529:Uridine / Q000493:pharmacokinetics
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D013662:Tea; D003561:Cytarabine; D013936:Thymidine; D014529:Uridine; D008727:Methotrexate