Anticryptococcal activity of amphotericin B-stimulated macrophages.
Amphotericin B (AmB) and its methyl ester derivative (AME) are immunoadjuvants with macrophage stimulating properties. Cultures containing AmB and murine peritoneal macrophages showed synergistic anticryptococcal activity. The antifungal activity was associated with AmB-stimulated macrophages and with their culture supernatants. Photoinactivation of the residual AmB in the macrophage culture supernatant did not result in the loss of antifungal activity. AmB-stimulated macrophage culture supernatants inhibited the growth of C. neoformans in a dose responsive manner and the activity was destroyed by incubation at 100 degrees C but not at 60 degrees C.
Aslanzadeh
J
J
Department of Medicine, Jewish Hospital, St. Louis.
Mormol
J S
JS
Little
J R
JR
D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D003455:Cryptococcus neoformans / Q000187:drug effects* / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D005260:Female; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology; D051379:Mice
D000666:Amphotericin B