Assessment of the relative skin sensitizing potency of 3 biocides using the murine local lymph node assay.
The relative skin-sensitizing potency of 3 biocides, 5-chloro-2-methyl-4-isothiazolin-3-one (the major active ingredient in Kathon CG), 1,2-benzisothiazolin-3-one and 2-methyl-4,5-trimethylene-4-isothiazolin-3-one, was assessed using the murine local lymph node assay. Potency was ranked according to the lowest dose of material which, following epicutaneous exposure, induced a significant proliferation of T lymphocytes in the draining lymph nodes. The results showed that 5-chloro-2-methyl-4-isothiazolin-3-one was able to induce proliferative activity at significantly lower dose levels than the other 2 biocides and that it may therefore be a more potent skin sensitizer.
Botham
P A
PA
ICI Central Toxicology Laboratory, Macclesfield, Cheshire, UK.
Hilton
J
J
Evans
C D
CD
Lees
D
D
Hall
T J
TJ
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003877:Dermatitis, Contact / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D008198:Lymph Nodes / Q000187:drug effects*; D051379:Mice; D008808:Mice, Inbred CBA; D011310:Preservatives, Pharmaceutical / Q000009:adverse effects*; D012867:Skin / Q000187:drug effects* / Q000276:immunology; D013844:Thiazoles / Q000009:adverse effects
D011310:Preservatives, Pharmaceutical; D013844:Thiazoles; C011506:2-methyl-4-isothiazolin-3-one; C011421:5-chloro-2-methyl-4-isothiazolin-3-one; C015699:1,2-benzisothiazoline-3-one