Experimental antitumor agents from Solanum indicum L.
beta-Sitosterol (SI-0), beta-sitosterol glucoside (SI-1), dioscin (SI-2), methyl protoprosapogenin A of dioscin (SI-3), methyl protodioscin (SI-4) and protodioscin (SI-5) were isolated and characterized from the whole plant of Solanum indicum L. (Solanaceae). Except for beta-sitosterol, these compounds have not been previously isolated from Solanum indicum L. Both CHCl3 soluble (SI-IV) and insoluble (SI-V) fractions of the ethanolic extract (SI-I) showed cytotoxicity on seven cancer cell lines: Colo-205 (colon), KB (nasopharynx), HeLa (uterine cervix), HA22T (hepatoma), Hep-2 (laryngeal epidermoid), GBM8401/TSGH (glioma) and H1477 (melanoma). The purified constituents, SI-2 and SI-4 showed more potent effects by DEA and MTT assay. SI-2,3,4 and 5 also demonstrated cytotoxicity on cultured C6 glioma cells by PRE assay, ans SI-3,4 and 5 showed a tumor inhibitory effect in vivo in C6 glioma cells. In addition, SI-2 had an inhibitory effect on the DNA synthesis of C6 glioma cells at 10 micrograms/ml.
Chiang
H C
HC
School of Pharmacy, College of Medicine, National Taiwan University, Taipei, R.O.C.
Tseng
T H
TH
Wang
C J
CJ
Chen
C F
CF
Kan
W S
WS
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D004144:Diosgenin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use; D004354:Drug Screening Assays, Antitumor; D005910:Glioma / Q000188:drug therapy; D008516:Medicine, Chinese Traditional; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use; D012855:Sitosterols / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D012503:Saponins; D012855:Sitosterols; C019357:dioscin; C026473:prosapogenin; C025473:gamma-sitosterol; D004144:Diosgenin