title: The nuclear:organelle distribution of chloroplast ribosomal proteins genes. Features of a cDNA clone encoding the cytoplasmic precursor of L11.
abstract: The majority of chloroplast ribosomal proteins are encoded in the nuclear genome. In order to characterize these proteins through their mRNA, we have previously constructed a spinach cDNA expression library and raised antisera to several spinach chloroplast ribosomal proteins. Here we describe the immuno isolation of cDNA clones encoding protein L11 and its chloroplast-targeting presequence. The cytoplasmic precursor form of L11 is 224 amino acid residues long (Mr 23,662); the mature L11 and the transit sequence are predicted to be of approximately 159 and approximately 65 residues, respectively. The predicted chloroplast L11 is significantly longer than the E coli L11, but similar (in size) to archaebacterial and yeast cytoplasmic L11. In sequence it is closer to E coli L11 (54% identity) than to the archaebacterial (32%) or yeast (23%) proteins. These results and the conservation of the contexts of the 3 methyl modified residues found in E coli L11 are discussed in the light of the endosymbiont theory and nuclear relocation of the rp/KAJL gene cluster.
authors.affiliation: Max-Planck-Institut für Molekuläre Genetik, Abteilung Wittmann, Berlin-Dahlem, Germany.
authors.forename: P M
authors.initials: PM
authors.lastname: Smooker
authors.forename: J
authors.initials: J
authors.lastname: Schmidt
authors.forename: A R
authors.initials: AR
authors.lastname: Subramanian
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000235:genetics; D005075:Biological Evolution; D002467:Cell Nucleus / Q000737:chemistry*; D002736:Chloroplasts / Q000737:chemistry*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010944:Plants / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics; D021382:Protein Sorting Signals / Q000737:chemistry; D012269:Ribosomal Proteins / Q000187:drug effects / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D011498:Protein Precursors; D021382:Protein Sorting Signals; D012269:Ribosomal Proteins; C027624:ribosomal protein L11