Expression of housekeeping genes in Hodgkin's disease lymph nodes.
Housekeeping genes, particularly actin, tubulin, and the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH), are widely used to estimate the amount and integrity of RNA in Northern blotting. In this work, the most reliable housekeeping gene for gene expression analysis of Hodgkin's disease (HD) lymph nodes was determined by comparing the conventional housekeeping genes, beta-actin, beta-tubulin, GAPDH, and the mouse gene LLRep3, that had been used previously in gene expression studies. It was found that the amounts of mRNA in these genes are very heterogeneous in HD lymph nodes. In contrast, their expression was relatively constant in tonsils undergoing a chronic inflammatory process. It is concluded that none of the housekeeping genes tested is suitable for the fine quantitative analysis of gene expression in HD lymph nodes.
Perfetti
V
V
Department of Internal Medicine, Institute of Clinica Medica II, University of Pavia, Italy.
Manenti
G
G
Dragani
T A
TA
D000199:Actins / Q000235:genetics; D015152:Blotting, Northern; D015972:Gene Expression Regulation, Neoplastic; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006689:Hodgkin Disease / Q000235:genetics*; D006801:Humans; D008198:Lymph Nodes / Q000503:physiopathology*; D012333:RNA, Messenger / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D014404:Tubulin / Q000235:genetics
D000199:Actins; D012333:RNA, Messenger; D014404:Tubulin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases