Effects of 5-fluorouracil on proliferating fibroblasts in vivo.
5-Fluorouracil (5-FU) is a potent inhibitor of the proliferation of fibroblasts, the main cell mediators of scar formation. The mechanism of the toxic effect of 5-FU on cancer cells is well known but unclear in nonmalignant cells. The histopathological effects of 5-FU on proliferating fibroblasts in vivo, 2 and 4 weeks after drug injection, are demonstrated here. An insertion of homologous fibroblasts into rabbits' vitreous followed by one injection of 1 mg 5-FU causes intracytoplasmic accumulation of vacuoles, morphological changes and inhibition of collagen deposition in the matrix.
Ophir
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Department of Ophthalmology, Hadassah University Medical Center, Jerusalem, Israel.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D005472:Fluorouracil / Q000494:pharmacology*; D008854:Microscopy, Electron; D011817:Rabbits; D012163:Retinal Detachment / Q000209:etiology; D013997:Time Factors; D014822:Vitreous Body / Q000187:drug effects
D005472:Fluorouracil