title: Effect of glutathione manipulation on prostaglandin synthesis in renal medullary homogenates.
abstract: 1. The effect of glutathione (GSH) manipulation on arachidonic acid (AA) metabolism in renal medullary (RM) homogenates was investigated. 2. Diethyl maleate (DEM) depleted GSH initially by 50% (P less than 0.05) and produced a general suppression (P less than 0.05) of all PGs with the exception of TXB2. GSH was further depleted during homogenization and a 30-min incubation period (P less than 0.01). 3. Adding glutathione monoethyl ester (GSH-MEE) (0, 0.8, 1.6 or 3.2 mmol/ml) to RM homogenates increased GSH (P less than 0.01) and decreased RM homogenates' PGs-synthesizing capability (P less than 0.05), with the exception of PGE2 and TXB2 at the highest concentration. 4. The results indicate that homogenization has a significant impact (P less than 0.05) on GSH concentration of the media and alterations in GSH concentration affect the profile and quantity of AA metabolites in renal medullary homogenates.
authors.affiliation: Department of Animal and Poultry Sciences, University of Arkansas, Fayetteville 72701.
authors.forename: H H
authors.initials: HH
authors.lastname: Nejad
authors.forename: K W
authors.initials: KW
authors.lastname: Beers
authors.forename: W G
authors.initials: WG
authors.lastname: Bottje
mesh_terms: D000818:Animals; D005978:Glutathione / Q000502:physiology*; D007679:Kidney Medulla / Q000378:metabolism*; D008297:Male; D008298:Maleates / Q000494:pharmacology; D011453:Prostaglandins / Q000096:biosynthesis*; D011817:Rabbits
chemical_list: D008298:Maleates; D011453:Prostaglandins; C014476:diethyl maleate; D005978:Glutathione