Arachidonate metabolism in bovine gallbladder mucosa.
Incubation of [1-14C] arachidonicacid (AA) with homogenates of bovine gallbladder mucosa in the presence or absence of reduced glutathione (GSH) generated large amounts of products that cochromatographed with PGE2 and 6-keto-PGF1 alpha and small amounts of products comigrating with PGF2 alpha, TxB2 and PGD2. In the presence of GSH PGE2-like material was predominantly produced while in the absence of GSH 6-keto-PGF1 alpha-like material was a major product. Identification of PGE2 was performed by its chemical conversion with NaBH4 or KOH treatments and by gas chromatography-mass spectrometry. Identification of 6-keto-PGF1 alpha was confirmed by gas chromatography-mass spectrometry. Gallbladder muscle produced predominantly 6-keto-PGF1 alpha in the presence or absence of GSH. The total amount of cyclooxygenase products was approximately 2 times higher in the mucosa than in the muscle. These results indicate that bovine gallbladder mucosa contains higher levels of fatty acid cyclooxygenase, and PGE2 isomerase and PGI2 synthetase in a proportion different from that in the muscle.
Hidaka
T
T
Department of Medical Biochemistry, Kurume University School of Medicine, Japan.
Nakano
M
M
Inokuchi
T
T
Sugiyama
M
M
Nishi
J
J
Ogura
R
R
D000818:Animals; D016718:Arachidonic Acid / Q000096:biosynthesis*; D002417:Cattle; D011464:Epoprostenol / Q000096:biosynthesis; D005704:Gallbladder / Q000378:metabolism*; D005978:Glutathione / Q000502:physiology; D009092:Mucous Membrane / Q000378:metabolism; D011457:Prostaglandins D / Q000096:biosynthesis; D011458:Prostaglandins E / Q000096:biosynthesis; D011460:Prostaglandins F / Q000096:biosynthesis; D013929:Thromboxane B2 / Q000096:biosynthesis
D011457:Prostaglandins D; D011458:Prostaglandins E; D011460:Prostaglandins F; D016718:Arachidonic Acid; D013929:Thromboxane B2; D011464:Epoprostenol; D005978:Glutathione