title: Artifacts in fluorescence emission spectroscopy related to Wood's anomaly.
abstract: Fluorescence emission spectra of porphyrins acquired with a monochromator using a 1500 line/mm holographic grating were affected by polarization-induced effects (Wood's anomaly) which affected the transmission of different wavelengths of light. This effect resulted in a blue-shift in the emission optimum, and distortions in the fluorescence spectrum at longer wavelengths. These anomalies could be eliminated by insertion of polarizers, orientation = 0 degrees, in emission and excitation paths. A monochromator with a 1200 line/mm holographic grating was essentially free from these distortions.
authors.affiliation: Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201.
authors.forename: D
authors.initials: D
authors.lastname: Kessel
mesh_terms: D016477:Artifacts; D011166:Porphyrins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D011166:Porphyrins