title: Effect of papaverine on endothelial cell harvest from canine external jugular veins.
abstract: Endothelial cell seeding procedures have been developed to line prosthetic bypass grafts used in peripheral vascular disease; however, because of current inefficient cell harvest techniques a high ratio of vein-to-graft area is necessary. This study was done to determine if the use of papaverine, a smooth muscle cell relaxant, would affect the number or viability of endothelial cells harvested from canine external jugular veins. Using a 0.12 mg/ml solution of papaverine in tissue culture medium to bathe the veins during dissection and excision, the viable cell yield was 2.20 +/- 1.16 (cells x 10(4)/cm2). A control group of veins using standard dissection technique gave a yield of 0.97 +/- 0.40 (p = 0.025). A second group of veins dissected while bathed in tissue culture medium alone gave a yield of 1.82 +/- 0.75, compared to a yield of 2.73 +/- 0.45 for papaverine harvested veins (p = 0.009). Percent cell viability was not significantly different for any of the groups: 73, 70, and 76% for papaverine, control and media only veins, respectively. The papaverine-harvested cells and those harvested with medium alone grew to 95% confluence in tissue culture in 9.8 +/- 1.1 and 9.9 +/- 0.9 days, respectively. Compared to conventional surgical techniques, use of papaverine more than doubled the endothelial cell yield from excised vein segments without adversely affecting viability or rate of growth in cell culture.
authors.affiliation: Department of Surgery, William Beaumont Hospital, Royal Oak, Mich.
authors.forename: T J
authors.initials: TJ
authors.lastname: Ranval
authors.forename: L E
authors.initials: LE
authors.lastname: Townsend
authors.forename: R
authors.initials: R
authors.lastname: Fietsam
authors.forename: P J
authors.initials: PJ
authors.lastname: Bendick
authors.forename: D M
authors.initials: DM
authors.lastname: Reitz-Vick
authors.forename: J L
authors.initials: JL
authors.lastname: Glover
mesh_terms: D000818:Animals; D002452:Cell Count; D002470:Cell Survival; D003470:Culture Media; D046508:Culture Techniques; D003584:Cytological Techniques; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology*; D007601:Jugular Veins / Q000166:cytology*; D010208:Papaverine / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D010208:Papaverine