[A simple way of introducing a transcription initiation signal in vector DNA].
A new effective method of site-specific mutagenesis in the close vicinity of unique restriction sites of plasmids, based on the use of two oligonucleotide primers, mutagenic and auxiliary, has been suggested. A site-specific insertion of Pribnow box (TATAATG) before promoterless gal operon of the promoter-testing plasmid pHD-001-14-11 has been performed with the yield of mutants up to 95%. Data on the gal operon expression and S1-nuclease mapping of the transcription start point indicate the formation of an active promoter in the region of the insertion.
Drutsa
V L
VL
Kaberdin
V R
VR
Koroleva
O N
ON
D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009876:Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D004247:DNA
Prostoĭ put' vvedeniia signala initsiatsii transkriptsii v vektornye DNK.