Immunological identification of uncoupling protein in interscapular "brown" adipose tissue of suckling and adult pipistrelle bats (Pipistrellus pipistrellus).
1. Interscapular adipose tissue of suckling and adult pipistrelle bats was examined for the presence of the 32,000 Mr "uncoupling protein" diagnostic of brown adipose tissue. 2. Following separation by SDS-polyacrylamide gel electrophoresis, mitochondrial proteins were blotted onto nitrocellulose and probed for uncoupling protein with an anti-(ground squirrel uncoupling protein) serum. 3. Immunoreactivity consistent with the presence of uncoupling protein was found in all samples of adipose tissue mitochondria from both suckling and adult bats. 4. It is concluded that interscapular adipose tissue in pipistrelle bats exhibits the critical biochemical criterion for being designated functionally "brown".
Trayhurn
P
P
Division of Biochemical Sciences, Rowett Research Institute, Bucksburn, Aberdeen, Scotland, UK.
Keith
J S
JS
Racey
P A
PA
Burnett
A M
AM
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis* / Q000276:immunology; D002685:Chiroptera; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015151:Immunoblotting; D007473:Ion Channels; D007774:Lactation / Q000502:physiology; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins; D008970:Molecular Weight; D011247:Pregnancy; D012589:Sciuridae; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D000071256:Uncoupling Protein 1