[Neutral alpha-mannosidase in human B- and T-lymphoid cells].
The presence of neutral alpha-mannosidase activity in normal and pathological lymphoid cells has been demonstrated. The specific activities of the enzyme in different cell types were similar with the exception of B-cells from B-CLL patients when it was a little higher. The activity of acid alpha-mannosidase was also determined in these lymphoid cells. The neutral to acid alpha-mannosidase activity ratio was different in B- and T-cells: in the former neutral alpha-mannosidase activity prevailed, whereas in the latter the predominance of acid alpha-mannosidase activity was apparent. Neutral alpha-mannosidases from pathological B- and T-cells were partially purified and their properties were investigated. In both cell types the enzyme was localized in the cytosol, was very labile and could be stabilized with Mn2+ and dithiothreitol. The enzyme was activated by Co2+ and inhibited by Zn2+ and EDTA. Swainsonine inhibited the B-cell neutral alpha-mannosidase somewhat more strongly in comparison with the T-cell enzyme.
Vlasova
A L
AL
Ushakova
N A
NA
Preobrazhenskaia
M E
ME
D001402:B-Lymphocytes / Q000187:drug effects / Q000201:enzymology*; D002458:Cell Fractionation; D003035:Cobalt / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D004229:Dithiothreitol / Q000494:pharmacology; D004789:Enzyme Activation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008232:Lymphoproliferative Disorders / Q000201:enzymology; D008345:Manganese / Q000494:pharmacology; D008361:Mannosidases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013601:T-Lymphocytes / Q000187:drug effects / Q000201:enzymology*; D015032:Zinc / Q000494:pharmacology; D043323:alpha-Mannosidase
D003035:Cobalt; D008345:Manganese; D008361:Mannosidases; D043323:alpha-Mannosidase; D015032:Zinc; D004229:Dithiothreitol
Neĭtral'naia alpha-mannozidaza B- i T-limfoidnykh kletok cheloveka.