[Effective synthesis and cloning of the human interleukin-2 gene and its analog: expression of the interleukin-2 gene in E. coli cells].
Artificial DNA fragments encoding human interleukin-2 (133 a.a.) and its analogue (deletion of 14 C-terminal a.a.) were prepared by means of the DNA polymerase I mediated extension of synthetic polynucleotides having short overlapping sequences at their 3'-ends. The fragments were cloned in specially designed pFH-type plasmids and then excised by the FokI and other restriction endonucleases to yield the subfragments with the structurally predetermined 5'-unique cohesive ends. The complete synthetic gene was constructed by one or two-step ligation. The expressed IL-2 was tested by analysing the T-cell proliferation activity of E.coli crude lisates containing the pEXIL2 expression plasmid.
Daniliuk
N K
NK
Seregin
S V
SV
Siniakov
A N
AN
Serpinskiĭ
O I
OI
Babkina
I N
IN
Urmanova
M A
MA
Riabinin
V A
VA
Pozdniakov
S G
SG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D006801:Humans; D007376:Interleukin-2 / Q000138:chemical synthesis / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D007376:Interleukin-2; D004247:DNA; D013936:Thymidine
Effektivnyĭ sintez i klonirovanie gena interleĭkina-2 cheloveka i egoanaloga: ékspressiia v kletkakh E. coli gena interleĭkina-2.