Overproduction of lignin-degrading enzymes by an isolate of Phanerochaete chrysosporium.
Phanerochaete chrysosporium is a white rot fungus which secretes a family of lignin-degrading enzymes under nutrient limitation. PSBL-1 is a mutant of this organism that generates the ligninolytic system under nonlimiting conditions during primary metabolism. Lignin peroxidase, manganese peroxidase, and glyoxal oxidase activities for PSBL-1 under nonlimiting conditions were 4- to 10-fold higher than those of the wild type (WT) under nitrogen-limiting conditions. PSBL-1 was still in the log phase of growth while secreting the enzymes, whereas the WT had ceased to grow by this time. As in the WT, manganese(II) increased manganese peroxidase activity in the mutant. However, manganese also caused an increase in lignin peroxidase and glyoxal oxidase activities in PSBL-1. Addition of veratryl alcohol to the culture medium stimulated lignin peroxidase activity, inhibited glyoxal oxidase activity, and had little effect on manganese peroxidase activity in PSBL-1, as in the WT. Fast protein liquid chromatography (FPLC) analysis shows production of larger amounts of isozyme H2 in PSBL-1 than in the WT. These properties make PSBL-1 very useful for isolation of large amounts of all ligninolytic enzymes for biochemical study, and they open the possibility of scale-up production for pratical use.
Orth
A B
AB
Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Denny
M
M
Tien
M
M
D000429:Alcohol Oxidoreductases / Q000096:biosynthesis*; D001592:Benzyl Alcohols / Q000494:pharmacology; D002912:Chrysosporium / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D003470:Culture Media; D005656:Fungal Proteins / Q000096:biosynthesis; D008031:Lignin / Q000378:metabolism*; D008345:Manganese / Q000494:pharmacology; D010544:Peroxidases / Q000096:biosynthesis*
D001592:Benzyl Alcohols; D003470:Culture Media; D005656:Fungal Proteins; D008345:Manganese; D008031:Lignin; D000429:Alcohol Oxidoreductases; C052371:glyoxal oxidase; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase; C042197:veratryl alcohol