title: Human clathrin heavy chain (CLTC): partial molecular cloning, expression, and mapping of the gene to human chromosome 17q11-qter.
abstract: The nucleotide sequence of a 916-bp human cDNA clone isolated from a human colon lambda gt11 cDNA library was determined. Sequence analysis showed this cDNA to have 88% homology to the nucleotide sequence of the heavy chain of rat clathrin. The deduced amino acid sequence was 98.7% identical to the rat sequence, a change of only four amino acids. The mRNA identified in both human and rat cells with the human clathrin clone revealed transcripts of approximately 6.5 kb, which is consistent with the predicted 180 kDa molecular weight of the clathrin heavy chain. Southern analysis of human/rodent somatic cell hybrids localized the human clathrin heavy chain gene (CLTC) to chromosome 17. Additional analyses using panels of human/rodent somatic cell hybrids with specific chromosomal translocations and deletions mapped the human clathrin heavy chain gene locus to 17q11-qter.
authors.affiliation: Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
authors.forename: G R
authors.initials: GR
authors.lastname: Dodge
authors.forename: I
authors.initials: I
authors.lastname: Kovalszky
authors.forename: O W
authors.initials: OW
authors.lastname: McBride
authors.forename: H F
authors.initials: HF
authors.lastname: Yi
authors.forename: M L
authors.initials: ML
authors.lastname: Chu
authors.forename: B
authors.initials: B
authors.lastname: Saitta
authors.forename: D G
authors.initials: DG
authors.lastname: Stokes
authors.forename: R V
authors.initials: RV
authors.lastname: Iozzo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D002966:Clathrin / Q000235:genetics*; D003001:Cloning, Molecular; D003106:Colon / Q000737:chemistry; D004247:DNA; D015870:Gene Expression; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
chemical_list: D002966:Clathrin; D004247:DNA