title: Cell-ingrowth in a silicone plombe. Interactions between biomaterial and scleral tissue after 8 years in situ: a SEM and TEM investigation.
abstract: A male patients (42 years) who had been treated for retinal detachment by the implantation of a silicone plombe into the sclera, returned to the clinic 8 years after implantation because of inflammation of the tissue and partial protrusion from the sclera. After removal of the plombe this was processed for TEM and SEM and examination of the plombe material after 8 years in situ could be carried out in order to get information about the cell-biomaterial interface. A large proportion of the silicone pores was filled with cellular material, including macrophages, giant cells and erythrocytes thus indicating a foreign body granuloma. The external surface of the pores showed a granular osmiophilic dense amorphous layer including extracellular debris. Engulfing of silicone particles by macrophages and the evidence of long-term tissue response suggest partial biodegradation of the silicone and certainly not complete inertness as was formerly claimed.
authors.affiliation: Laboratory for Histology and Cell Biology, University of Groningen, The Netherlands.
authors.forename: D
authors.initials: D
authors.lastname: Kalicharan
authors.forename: W L
authors.initials: WL
authors.lastname: Jongebloed
authors.forename: G
authors.initials: G
authors.lastname: van der Veen
authors.forename: L I
authors.initials: LI
authors.lastname: Los
authors.forename: J G
authors.initials: JG
authors.lastname: Worst
mesh_terms: D000328:Adult; D001672:Biocompatible Materials / Q000009:adverse effects; D015745:Granuloma, Foreign-Body / Q000209:etiology / Q000473:pathology*; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D012163:Retinal Detachment / Q000601:surgery; D012590:Sclera / Q000648:ultrastructure; D012591:Scleral Buckling / Q000009:adverse effects; D015423:Scleritis / Q000209:etiology / Q000473:pathology*; D012826:Silicone Elastomers / Q000009:adverse effects*
chemical_list: D001672:Biocompatible Materials; D012826:Silicone Elastomers