title: [Interaction of alpha-(125)latrocrustotoxin with nerve cell membranes from the river crab Astacus astacus].
abstract: alpha-Latrocrustatoxin, the crustacean-specific neurotoxin from the venom of the black widow spider Latrodectus mactans tredecimguttatus was radioactively labelled with Bolton-Hunter reagent to the specific activity of 160 Ci/mmol with retention of the biological activity. A highly specific binding of radioactive toxin on plasmatic membranes from the crayfish Astacus astacus nerve cells with Bmax = 0.04 pmol binding toxin/mg membrane protein and Kd = 0.7 x 10(-10) M was demonstrated.
authors.forename: V G
authors.initials: VG
authors.lastname: Krasnoperov
authors.forename: O G
authors.initials: OG
authors.lastname: Shamotienko
authors.forename: E V
authors.initials: EV
authors.lastname: Grishin
mesh_terms: D000818:Animals; D001665:Binding Sites; D003386:Brachyura; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D013111:Spider Venoms / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D008565:Membrane Proteins; D013111:Spider Venoms; C106203:latrocrustatoxin
vernacular_title: Vzaimodeĭstie alpha-(125)latrokrustotoksina s plazmaticheskimi membranami nervnykh kletok rechnogo raka Astacus astacus.