Liquid chromatographic determination of chlortetracycline hydrochloride in ruminant and poultry/swine feeds.
A liquid chromatographic (LC) method is described for the determination of chlortetracycline hydrochloride (CTC) in poultry/swine and ruminant feeds in the 10-100 ppm range and in premix. CTC is extracted from ground feed/premix with acidified acetone, and the extract is filtered through a Millex-HV filter or disposable C18 column. The filtrate is partitioned with methylene chloride when additional cleanup is necessary. A Nova-Pak C18 column is used for LC separation with determination at 370 nm. The average recovery of CTC from premix was 95% with a standard deviation (SD) of 1.70 and a coefficient of variation (CV) of 1.79%. The overall average recovery from feeds was 77% with an SD of 3.18 and a CV of 4.10%.
Holland
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Food and Drug Administration, Animal Drug Research Center, Denver, CO 80225-0087.
Faul
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Roybal
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Munns
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Shimoda
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D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002645:Chickens; D002751:Chlortetracycline / Q000032:analysis*; D002853:Chromatography, Liquid; D012418:Ruminants; D013552:Swine
D002751:Chlortetracycline