Avian tandem repeats: nuclear protein binding sites and the origins of short tandem repetitive DNAS.
A 14 bp repeat isolated from the genome of a Mallard duck (Anas platyrhynchos) and a 13 bp repeat isolated from the genome of a Muscovy duck (Cairina moschata) were both tandem and genus-specific. There were 2.1 x 10(6) copies of the 14 bp repeat in the Mallard duck genome and 1.3 x 10(6) copies of the 13 bp repeat in the Muscovy duck genome. These two repeats were not obviously homologous but each contains different transcription protein binding sites. Experimentally, the two repeats were also observed to bind nuclear proteins specifically. A sequence-selective mechanism for the expansion of short tandem repeats from single ancestral sequences containing nuclear protein binding sites is presented.
Zhu
X Y
XY
School of Biology, Flinders University, Australia.
Testori
A
A
Oh
S
S
Skinner
J D
JD
Burgoyne
L A
LA
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004372:Ducks / Q000235:genetics*; D015698:Genomic Library; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D011485:Protein Binding; D012091:Repetitive Sequences, Nucleic Acid
D009687:Nuclear Proteins; D004247:DNA