[Activation of the resident microbial flora as a possibility for the improvement of humoral antibody potentials].
The effect of a parenteral application of an adjuvant (Propionibacterium acnes) and an adjuvant/antigen combination (Staphylococcus aureus Cowan I/Al(OH)3, Pseudomonas aeruginosa/Al(OH)3 respectively, was tested with regard to the improvement of antibacterial resistance. Parameter was the humoral antibody production (IgG) of rabbits against six facultative pathogen bacterial species (St. aureus, Sc. faecium, Bac. cereus, P. multocida, E. coli, Ps. aeruginosa) and against sheep rod blood cell membranes. The three methods of treatment led to a significant enhancement of IgG-antibodies against the particular homologous antigen. In addition to this specific reaction the application of adjuvant/antigen combinations provoked a significant enhancement of antibodies also against heterologous antigens (P. multocida, Bac. cereus, sheep red blood cell membranes). The application of P. acnes had no distinct effect on the antibody titer against the different antigen preparations. In order to analyse the immune response qualitatively, a greater part of serum samples was examined by immuno blot technique. The number of partial antigens recognized by the sera increased during the experiments but the rise did generally not relate to developments of antibody titers. Nevertheless, the Ps. aeruginosa/Al(OH)3-treatment seemed to improve the ability of sera to detect antigenic determinants of heterologous bacterial species.
Lübke
A
A
Institut für Mikrobiologie und Tierhygiene, Freien Universität Berlin.
Hellmann
E
E
D000276:Adjuvants, Immunologic; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000917:Antibody Formation; D004910:Erythrocyte Membrane / Q000276:immunology; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis*; D008297:Male; D011817:Rabbits
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D007074:Immunoglobulin G
Aktivierung der residenten Keimflora als Möglichkeit zur Verbesserung des humoralen Antikörperpotentials.