The possible involvement of protein synthesis in the injection of PL-1 phage genome into its host, Lactobacillus casei.
The process of genome DNA injection, after adsorption, by phage PL-1 into host cells of Lactobacillus casei was monitored by using the electron microscope. Injection of DNA was inhibited by the protein-synthesis inhibitors chloramphenicol and erythromycin at concentrations where the colony-forming ability of cells not infected by phage was unaffected. The results suggest that protein synthesis may be involved in some way in the process of genome injection.
Watanabe
K
K
Microbiology Laboratory, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan.
Shirabe
M
M
Nakashima
Y
Y
Kakita
Y
Y
D001426:Bacterial Proteins / Q000096:biosynthesis*; D001435:Bacteriophages / Q000235:genetics* / Q000648:ultrastructure; D016679:Genome, Viral; D007780:Lacticaseibacillus casei / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron
D001426:Bacterial Proteins