Purification of domoic acid from toxic blue mussels (Mytilus edulis) and phytoplankton.
Domoic acid was the primary neurotoxin in blue mussel (Mytilus edulis) that caused poisoning in humans. Further research showed that the algae, Nitzschia pungens, was the source of this toxin. In this study, a method for the extraction and purification of domoic acid from contaminated mussels and phytoplankton was developed. Domoic acid was extracted from these sources by treatment with a mixture of chloroform and methanol (1:2, v/v). The resulting extract was subjected to ultrafiltration through a PM1 Millipore filter, followed by repeated high-performance liquid chromatography on a reversed-phase column. The purity and yield of domoic acid prepared by this method are compared with two previously described methods of extraction. The current method is relatively simple, rapid, and results in improved recovery with comparable purity of domoic acid.
Nijjar
M S
MS
Department of Anatomy and Physiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada.
Grimmelt
B
B
Brown
J
J
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005506:Food Contamination / Q000032:analysis; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000633:toxicity; D008387:Marine Toxins / Q000302:isolation & purification*; D051379:Mice; D010839:Phytoplankton / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D008387:Marine Toxins; C012301:domoic acid; D007608:Kainic Acid