title: A chloramphenicol-streptomycin-resistance plasmid from a clinical strain of Staphylococcus sciuri and its structural relationships to other staphylococcal resistance plasmids.
abstract: A 5.1-kb plasmid, designated pSCS12, isolated from a naturally occurring Staphylococcus sciuri conferred resistance to chloramphenicol (CmR) and streptomycin (SmR). Restriction endonuclease analyses of pSCS12 revealed partial structural homologies to the CmR-plasmids pC221 from S. aureus and pSCS1 from S. intermedius, to the SmR-plasmids pSAI-1 from S. hyicus and pS194 from S. aureus, as well as to the CmR/SmR plasmid pSK68 from S. aureus. Southern-blot hybridization with specific CmR- and SmR-gene probes confirmed these similarities and allowed the mapping of the CmR- and SmR-determinants in the S. sciuri plasmid pSCS12. These observations lead to the suggestion that CmR/SmR-plasmids, such as pSCS12, may have evolved from CmR- and SmR-plasmids by interplasmidic recombination.
authors.affiliation: Institut für Bakteriologie und Immunologie, Justus Liebig Universität Giessen, F.R.G.
authors.forename: S
authors.initials: S
authors.lastname: Schwarz
authors.forename: S
authors.initials: S
authors.lastname: Grölz-Krug
mesh_terms: D002702:Chloramphenicol Resistance / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D011815:R Factors / Q000235:genetics*; D015183:Restriction Mapping; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics*; D013307:Streptomycin / Q000494:pharmacology; D014162:Transfection
chemical_list: D013307:Streptomycin