Xanthine oxidase formation during experimental ischemia of the equine small intestine.
We hypothesized that xanthine oxidase plays a role in the postischemic reperfusion injury in the equine small intestine. Under anesthesia, four horses and two ponies underwent ischemic strangulating obstructions of segments of the proximal jejunum, mid-jejunum and ileum. Prior to vascular occlusion, and at 1 h and 2 h of ischemia, full-thickness intestinal biopsies were collected for histopathological evaluation and for determination of combined xanthine dehydrogenase (XDH) plus xanthine oxidase (XO) activity, and XO activity alone. The level of XO activity was expressed in percentage according to the ratio of XO/(XDH + XO). We found a nearly threefold increase in the combined level of XDH plus XO activity from ileum to duodenum (p less than 0.04). However, the preischemic level of % XO activity did not vary significantly (p = 0.61) between segments of jejuno-ileum. Likewise, no significant difference was noted between intestinal segments after ischemia. Therefore, the data from all intestinal segments were pooled for each time and analyzed using Wilcoxon's signed rank test (one-tailed). Compared to the pre-ischemic level of % XO activity (median 27%), the % XO activity increased after 1 h of ischemia (median 37.0%), reaching statistical significance (p = 0.016). There were no statistical differences between the preischemic % XO activity and the % XO activity in non-ischemic bowel at the end of the anesthetic period. During ischemia, % XO activity increased, which lends credence to the importance of xanthine oxidase in previously-documented reperfusion injury in the equine small intestine.
Prichard
M
M
Department of Clinical Sciences, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Ducharme
N G
NG
Wilkins
P A
PA
Erb
H N
HN
Butt
M
M
D000818:Animals; D001706:Biopsy / Q000662:veterinary; D006734:Horse Diseases / Q000201:enzymology* / Q000209:etiology; D006736:Horses; D007421:Intestine, Small / Q000098:blood supply* / Q000201:enzymology / Q000473:pathology; D007511:Ischemia / Q000150:complications / Q000201:enzymology / Q000662:veterinary*; D015427:Reperfusion Injury / Q000209:etiology / Q000662:veterinary*; D014968:Xanthine Dehydrogenase / Q000378:metabolism; D014969:Xanthine Oxidase / Q000096:biosynthesis*
D014968:Xanthine Dehydrogenase; D014969:Xanthine Oxidase