Artifacts in fluorescence emission spectroscopy related to Wood's anomaly.
Fluorescence emission spectra of porphyrins acquired with a monochromator using a 1500 line/mm holographic grating were affected by polarization-induced effects (Wood's anomaly) which affected the transmission of different wavelengths of light. This effect resulted in a blue-shift in the emission optimum, and distortions in the fluorescence spectrum at longer wavelengths. These anomalies could be eliminated by insertion of polarizers, orientation = 0 degrees, in emission and excitation paths. A monochromator with a 1200 line/mm holographic grating was essentially free from these distortions.
Kessel
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Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201.
D016477:Artifacts; D011166:Porphyrins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D011166:Porphyrins