Valinomycin and its interaction with ions in organic solvents, detergents, and lipids studied by Fourier transform IR spectroscopy.
The structure of valinomycin in a range of organic solvents of varying polarity and in detergent and lipid dispersions has been studied by Fourier transform ir spectroscopy. In solvents of low polarity such as chloroform, ir spectra of valinomycin are fully consistent with the bracelet structure proposed on the basis of nmr spectroscopy, showing a single narrow amide I component attributable to the presence of beta-turns and a single band arising from nonhydrogen-bonded ester C = O groups. K+ complexation results in a downward shift in the amide I band frequency, indicating an increase in the strength of the amide hydrogen bonds, along with a shift to lower frequencies of the ester C = O absorption due to a reduction in electron density in these bonds upon complexation. Identical results were obtained with NH4+, a finding not previously reported. In solvents of both medium (CHCl3/DMSO 3:1) and high (pure DMSO) polarity, we find evidence of significant disruption of the internal hydrogen-bonding network of the peptide and the appearance of a band suggesting the presence of free amide C = O groups. In such solvents, complexation with K+ and NH4+ was not observed. The structure of valinomycin in detergent micelles resembles that in nonpolar organic solvents. However, changes were found in the amide I and ester carbonyl maxima as 2H2O penetrated the micelle which suggest significant interaction between the solvent and peptide. Complexation with K+ was reduced in cationic detergent micelles as a result of a decrease in the effective K+ concentration due to charge repulsion at the micelle surface.(ABSTRACT TRUNCATED AT 250 WORDS)
Jackson
M
M
Steacie Institute for Molecular Sciences, National Research Council Canada, Ottawa.
Mantsch
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D000595:Amino Acid Sequence; D002725:Chloroform / Q000737:chemistry; D003902:Detergents / Q000737:chemistry; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D005583:Fourier Analysis; D007477:Ions; D008051:Lipid Bilayers / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D008823:Micelles; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D011188:Potassium / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D014634:Valinomycin / Q000737:chemistry*
D003902:Detergents; D007477:Ions; D008051:Lipid Bilayers; D008055:Lipids; D008823:Micelles; D000644:Quaternary Ammonium Compounds; D012997:Solvents; D014634:Valinomycin; D002725:Chloroform; D011188:Potassium; D004121:Dimethyl Sulfoxide