title: Isolation, sequence and bacterial expression of a cDNA for chalcone synthase from the cultured cells of Pueraria lobata.
abstract: cDNA clones for chalcone synthase (CHS) of Pueraria lobata cultured cells were isolated by screening the cDNA library using CHS cDNA of Phaseolus vulgaris as a probe. Analysis of nucleotide sequences of the cloned cDNA revealed a 1170-bp open reading frame that encoded a 390-amino acid polypeptide with an Mr of 43,000. The full-length cDNA was cloned into the expression vector pT7-7. CHS activity was found in the crude extracts of transformed E. coli after induction and two protein bands of ca. 43 and 34 kd were hybridized with anti-persley CHS antiserum.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Nakajima
authors.forename: T
authors.initials: T
authors.lastname: Akiyama
authors.forename: T
authors.initials: T
authors.lastname: Hakamatsuka
authors.forename: M
authors.initials: M
authors.lastname: Shibuya
authors.forename: H
authors.initials: H
authors.lastname: Noguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Ebizuka
authors.forename: U
authors.initials: U
authors.lastname: Sankawa
mesh_terms: D000217:Acyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D010944:Plants / Q000201:enzymology*
chemical_list: D004247:DNA; D000217:Acyltransferases; C020141:flavanone synthetase