Production and partial characterization of monoclonal antibodies specific for the gamonts of Eimeria tenella.
Thirteen hybridomas secreting monoclonal antibodies (Mabs) specific for the sexual stages (gamonts) of Eimeria tenella were produced by fusing spleen cells of gamont-immunized RBF/Dn mice with FOX-NY myeloma cells. A Mab subisotype profile revealed 1 IgG2a and 12 IgG1. All Mabs demonstrated a similar binding pattern when incubated with parasitic gamonts as determined by the indirect fluorescent antibody test. Ascitic fluid containing Mab (GD9 (IgG1) was produced and used to immunize chicks passively per os. There was a 34% decrease (P less than 0.05) in oocyst output from immunized chicks when compared to control chicks. Passively immunized chicks also had reduced cecal lesion scores when compared to control chicks. These results suggest that Mab GD9 partially inhibited the fertilization process of E. tenella.
Larsen
N C
NC
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan 84322-5600.
Rasmussen
K R
KR
Healey
M C
MC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000913:Antibodies, Protozoan / Q000276:immunology; D000918:Antibody Specificity; D002432:Cecum / Q000469:parasitology; D002645:Chickens / Q000469:parasitology; D003048:Coccidiosis / Q000517:prevention & control / Q000662:veterinary*; D016786:Eimeria tenella / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D007116:Immunization, Passive; D007411:Intestinal Diseases, Parasitic / Q000517:prevention & control / Q000662:veterinary; D011201:Poultry Diseases / Q000517:prevention & control*
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan