Interferon induced increases in c-myc expression in a human breast carcinoma cell line.
Previous studies from our laboratory have indicated that interferon-gamma inhibits the growth of a human breast carcinoma cell line, MDA 468. We measured steady state levels of c-myc mRNA to determine if the antiproliferative effect of IFN-gamma was mediated by changes in the expression of this proto-oncogene. C-myc message levels increased after 24 hours of IFN treatment, peaked at 48 hours, but remained elevated through 96 hours of treatment. The increase in c-myc mRNA was observed with as little as 10 U/ml of IFN-gamma. The stability of mRNA was enhanced in interferon treated cells. These data suggest that IFN-gamma increased expression of c-myc mRNA, but decreased cell growth, and point out the complexities of the relationship of the expression of this protooncogene to cell growth.
Hamburger
A W
AW
University of Maryland Cancer Center, Division of Cell and Molecular Biology, Baltimore 21201.
Pinnamaneni
G
G
D001943:Breast Neoplasms / Q000235:genetics*; D003513:Cycloheximide / Q000494:pharmacology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects* / Q000235:genetics; D016259:Genes, myc; D006207:Half-Life; D006801:Humans; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000378:metabolism*; D014407:Tumor Cells, Cultured
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D007371:Interferon-gamma; D003513:Cycloheximide