[The effects of calcium concentration of reperfusion solution upon myocardial protection].
The effects of several calcium concentrations in reperfusion solution were studied. Experimental time course was as followed: 20 min working perfusion, 3 min cardioplegic infusion with St. Thomas cardioplegic solution (STS) followed by global ischemia for 30 min at 37.5 degrees C, 15 min early Langendorff reperfusion with reperfusion solution and 5 min late reperfusion with Krebs Henseleit bicarbonate buffer [( Calcium] = 2.5 mM), followed by 20 min working perfusion. Percent recoveries of aortic flow at the Ca concentration of 0, 0.1, 0.6, 1.2, 1.8, 2.5 mM were 0, 14 +/- 1, 43 +/- 4, 64 +/- 3, 55 +/- 2, 59 +/- 1 (%), respectively. Our data indicated that reperfusion solution with less than 1.2 mM calcium reduced the protective properties of STS.
Ohashi
T
T
Department of Cardiovascular Surgery, National Cardiovascular Center, Oska, Japan.
Yamamoto
F
F
Yamamoto
H
H
Ichikawa
H
H
Komai
H
H
Shibata
T
T
Nakajima
N
N
Kawashima
Y
Y
D000818:Animals; D002118:Calcium / Q000032:analysis / Q000494:pharmacology*; D002314:Cardioplegic Solutions / Q000032:analysis / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D006324:Heart Arrest, Induced; D066298:In Vitro Techniques; D008297:Male; D015425:Myocardial Reperfusion; D051381:Rats; D011919:Rats, Inbred Strains
D002314:Cardioplegic Solutions; D002118:Calcium