Flow cytometric studies of IL-4-stimulated expression of the CD25 antigen by quiescent human B lymphocyte subpopulations.
The membrane immunoglobulin (mIg) phenotype of high-density human tonsillar B lymphocytes which display elevated levels of the CD25 marker in response to treatment with interleukin-4 (IL-4) has been investigated. IL-4 promotes elevation of CD25 levels in B lymphocytes expressing membrane IgM (mIgM), mIgD, mIgG and mIgA. Three-colour flow cytometric analyses indicate that 25-35% of high-density tonsillar B cells are simultaneously positive for mIgM, mIgD and CD25 following IL-4 stimulation. The hypothesis that B cells could respond to IL-4 by up-regulation of either CD23 or CD25, but not both simultaneously, was evaluated. Three-colour flow cytometric studies indicated that approximately one-third of the B-lymphocyte population was simultaneously positive for the CD19, CD23 and CD25 antigens following stimulation with IL-4. These data are consistent with the proposal that IL-4 can promote expression of CD23 and CD25 antigens in the same B lymphocyte.
Butcher
R D
RD
Department of Biochemistry, University of Glasgow, U.K.
Cushley
W
W
D016175:B-Lymphocyte Subsets / Q000276:immunology*; D002648:Child; D005434:Flow Cytometry; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007072:Immunoglobulin D / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D015847:Interleukin-4 / Q000276:immunology*; D014066:Palatine Tonsil / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D015375:Receptors, Interleukin-2 / Q000032:analysis*
D007070:Immunoglobulin A; D007072:Immunoglobulin D; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell; D015375:Receptors, Interleukin-2; D015847:Interleukin-4