Physical mapping of the Esterase-6 locus of Drosophila melanogaster.
The Esterase-6 gene locus of Drosophila melanogaster although well-characterized, has not been definitely mapped by in situ hybridization. In this paper, a high resolution in situ hybridization protocol using an avidin/biotinylated-horseradish peroxidase/diaminobenzidine system was adopted to refine the physical map position of the Esterase-6 locus. Clarity of signal, detail of banding pattern and absence of background allowed the assignment of a 1.8 kb cDNA encoding Esterase-6 to three bands within subsections 69 A1-A3 on the left arm of polytene chromosome 3. These data refine earlier deletion mapping and low resolution in situ hybridization results, which assigned Esterase-6 to 69A1-A5. The potential use of this high resolution in situ hybridization technique in the analysis of the physical organization of the Esterase-6 gene duplication and surrounding region is discussed.
Procunier
W S
WS
Department of Entomology, Michigan State University, East Lansing 48824-1115.
Smith
J J
JJ
Richmond
R C
RC
D000818:Animals; D001710:Biotin; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D002874:Chromosome Mapping; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010784:Photomicrography; D012469:Salivary Glands / Q000648:ultrastructure
D001710:Biotin; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase