[The effective use of superoxide dismutase from human erythrocytes in the late stages of experimental influenza infection].
The role of reactive oxygen species (ROS) in the pathogenesis of influenza infection was studied. Bronchoalveolar lavage and serum of influenza-infected mice were shown to produce ROS following the addition of phorbole ether-PMA. The generation of ROS was the maximum in 5-6 days after virus inoculation which coincided with the beginning of animal deaths. The main form of ROS was hypochloride generated by neutrophil meyloperoxidase. Treatment of mice with superoxide dismutase (SOD) in the late period of infection (as early as 12 hours before death) led to an increase in the survival rate of mice by 30-50% depending upon the dose of the preparation. The survival rate in the controls was 10%. The treatment of mice early in the infection led to an increase in the survival rate by 80%. The use of SOD conjugates with albumin or polymers led not only to an increase in the survival rate but also to a delay in deaths. The effective use of SOD is believed to depend upon the inhibition of neutrophil influx to the lungs.
Sharonov
B P
BP
Dolganova
A V
AV
Kiselev
O I
OI
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D004353:Drug Evaluation, Preclinical; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D009980:Influenza A virus; D007251:Influenza, Human / Q000188:drug therapy* / Q000378:metabolism / Q000401:mortality; D008163:Luminescent Measurements; D051379:Mice; D008808:Mice, Inbred CBA; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D013482:Superoxide Dismutase / Q000302:isolation & purification / Q000627:therapeutic use*
D013482:Superoxide Dismutase
Effektivnoe ispol'zovanie superoksiddismutazy iz éritrotsitov cheloveka na pozdnikh stadiiakh éksperimental'noĭ grippoznoĭ infektsii.