Inhibition of lipid peroxidation of lecithin liposomes kept in a pH-stat system near neutral pH.
During 5 days of autoxidation of egg lecithin liposomes in nonbuffered saline pH dropped from an initial value of 7.4 to 4.5. A linear relationship between oxidation index and pH was obtained. Lipid peroxidation, monitored as conjugated diene and TBA-reactive products, was inhibited significantly by keeping the samples under pH-controlled conditions (7.4 +/- 0.5), compared to controls. Obtained results indicate that the buffering capacity of Tris and Hepes buffers may play a role in their recently reported (D. Fiorentini et al. (1989) Free Radical Res. Commun., 6, 243) inhibitory action against lipid peroxidation of lecithin liposomes.
Misík
V
V
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Svajdlenka
E
E
Filípek
J
J
Gergel
D
D
Ondrias
K
K
D002021:Buffers; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry*
D002021:Buffers; D008054:Lipid Peroxides; D008081:Liposomes; D010713:Phosphatidylcholines