A rapid colorimetric assay for heparinase activity.
A rapid, sensitive, assay for enzymes that degrade heparin is described. The procedure is based on the interference of heparin with color development during the interaction of protein with the dye Coomassie brilliant blue. The loss of this property when the glycosaminoglycan is degraded by heparinase can be used to quantify activity of the enzyme in pure form, or in complex biological samples such as tissue homogenates or serum. The assay is also suitable for studying dependence of heparinase activity under conditions such as varying pH and temperature.
Khan
M Y
MY
Department of Cell Biology and Anatomy, New York Medical College, Valhalla 10595.
Newman
S A
SA
D003124:Colorimetry / Q000379:methods; D019763:Heparin Lyase; D006863:Hydrogen-Ion Concentration; D008832:Microchemistry / Q000379:methods; D011133:Polysaccharide-Lyases / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D011133:Polysaccharide-Lyases; D019763:Heparin Lyase