Duplication of both xyl catabolic operons on TOL plasmid pWW15.
Pseudomonas fluorescens MT15 is the host of the large (250 kbp) TOL plasmid pWW15. We have shown by a combination of hybridization, molecular cloning and enzyme assay that pWW15 carries two distinct regions which share homology with the upper pathway operons (xylCMABN) of other TOL plasmids and two distinct regions which are homologous to the meta pathway operons (xylXYZLTEGFJQKIH) of other TOL plasmids. Both the areas of homology to the upper pathway operons appear to carry all of the structural genes for the three catabolic enzymes of the operon. One of the regions of meta pathway operon homology encodes a complete functional pathway, but the second is incomplete and appears to carry only the genes from xylF downstream.
O'Donnell
K J
KJ
School of Biological Sciences, University of Wales, Bangor, Gwynedd, UK.
Williams
P A
PA
D050557:Catechol 2,3-Dioxygenase; D003001:Cloning, Molecular; D049308:Dioxygenases; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D009876:Operon; D010105:Oxygenases / Q000235:genetics*; D010957:Plasmids; D011551:Pseudomonas fluorescens / Q000201:enzymology / Q000235:genetics*; D015183:Restriction Mapping; D014050:Toluene / Q000378:metabolism
D014050:Toluene; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase