[Effects of verapamil on the activity of Ca2+, Mg2+-dependent endonucleases in the nuclei of the dog liver during prolonged hypovolemic shock].
In the experiments of 23 mongrel anesthetized dogs it was established that the blockade of calcium channels with verapamil (0.1 mg/kg) significantly decreased the activity of Ca2+, Mg(2+)-dependent endonucleases in the nuclei of canine liver (after four hours of arterial hypotension, arterial pressure--40 mm Hg). Verapamil was injected intravenously 30 min before the beginning of haemorrhage. The infusion of verapamil to intact animals does not change the activity of the investigated DNA-endonucleases. The obtained experimental data demonstrate that verapamil effectively blocks the activity of endonucleases in the nuclei of canine liver in the conditions of an increasing permeability of hepatocytes' membranes which integrity is deranged during hypovolemic shock. The present investigation reveals a new molecular mechanism of defense of hepatocytes from the damaging effect of one of the pathogenetic factors of a terminal process, namely an increase in the activity of Ca2+, Mg(2+)-dependent endonucleases in the nuclei of the liver.
Kozhura
V L
VL
Smirnova
N V
NV
Novoderzhkina
I S
IS
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology*; D004285:Dogs; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D005260:Female; D008099:Liver / Q000201:enzymology*; D008297:Male; D012769:Shock / Q000201:enzymology*; D013997:Time Factors; D014700:Verapamil / Q000494:pharmacology*
D014700:Verapamil; D004706:Endodeoxyribonucleases; C029367:calcium magnesium dependent endodeoxyribonuclease
Vliianie verapamila na aktivnost' Ca2+, Mg2+-zavisimykh éndonukleaz v iadrakh pecheni sobak pri dlitel'nom gipovolemicheskom shoke.