Generation and fluorescent in situ hybridization mapping of yeast artificial chromosomes of 1p, 17p, 17q, and 19q from a hybrid cell line by high-density screening of an amplified library.
A yeast artificial chromosome (YAC) library has been constructed from a somatic cell hybrid containing a t(1p;19q) chromosome and chromosome 17. After amplification, part of this library was analyzed by high-density colony filter screening with a repetitive human DNA probe (Alu). The human YACs distinguished by the screening were further analyzed by Alu fingerprinting and Alu PCR. Fluorescent in situ hybridization (FISH) was performed to localize the YACs to subchromosomal regions of chromosome 1p, 17, or 19q. We have obtained a panel of 123 individual YACs with a mean size of 160 kb, and 77 of these were regionally localized by FISH: 33 to 1p, 10 to 17p, 25 to 17q, and 9 to 19q. The YACs cover a total of 19.7 Mb or 9% of the 220 Mb of human DNA contained in the hybrid. No overlapping YACs have yet been detected. These YACs are available upon request and should be helpful in mapping studies of disease loci, e.g., Charcot-Marie-Tooth disease, Miller-Dieker syndrome, hereditary breast tumor, myotonic dystrophy, and malignant hyperthermia.
Driesen
M S
MS
Department of Human Genetics, State University Leiden, The Netherlands.
Dauwerse
J G
JG
Wapenaar
M C
MC
Meershoek
E J
EJ
Mollevanger
P
P
Chen
K L
KL
Fischbeck
K H
KH
van Ommen
G J
GJ
D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D002878:Chromosomes, Human, Pair 1; D002886:Chromosomes, Human, Pair 17; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular; D016172:DNA Fingerprinting; D005784:Gene Amplification; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D006822:Hybrid Cells; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid