title: Three-dimensional reconstruction of rabbit-derived Pneumocystis carinii from serial-thin sections. I: Trophozoite.
abstract: The highly complex ultrastructural morphology of the endomembrane system in Pneumocystis carinii led us to perform three-dimensional reconstruction from serial-thin sections using the CATIA (Conception Assistée Tridimensionnelle Inter Active) Dassault system program. The three-dimensional reconstruction of a small trophozoite made it possible to better understand the morphological relationship among organelles and to suggest cytophysiological hypotheses. By reconstructing other parasite stages, we gathered information about the evolution of organelles during the life cycle and about their physiology.
authors.affiliation: I.N.S.E.R.M. (U. 42), Unité de Biologie et Biochimie Parasitaires et Fongiques, domaine du C.E.R.T.I.A., Villeneuve d'Ascq, France.
authors.forename: F
authors.initials: F
authors.lastname: Palluault
authors.forename: B
authors.initials: B
authors.lastname: Pietrzyk
authors.forename: E
authors.initials: E
authors.lastname: Dei-Cas
authors.forename: C
authors.initials: C
authors.lastname: Slomianny
authors.forename: B
authors.initials: B
authors.lastname: Soulez
authors.forename: D
authors.initials: D
authors.lastname: Camus
mesh_terms: D000818:Animals; D003196:Computer Graphics; D007091:Image Processing, Computer-Assisted; D007425:Intracellular Membranes / Q000648:ultrastructure; D011010:Pneumocystis / Q000254:growth & development / Q000648:ultrastructure*; D011817:Rabbits