The effect of nicotine on cell proliferation and synthesis of secreted proteins in BALB/C 3T3 cells.
The alkaloid nicotine induces a dose-dependent inhibition of cell proliferation and morphological change when added to BALB/C 3T3 cells. Significant differences were observed between control and nicotine-treated cells with respect to the newly synthesized secreted proteins by using heparin-agarose column chromatography. Both the anticellular and protein synthesis modulating activities of nicotine were affected by the degree of confluence of cells, suggesting a complex mode of action of nicotine in mammalian cells.
Konno
S
S
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595.
Oronsky
B T
BT
Semproni
A R
AR
Wu
J M
JM
D016475:3T3 Cells; D000818:Animals; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects*; D002847:Chromatography, Agarose; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D006133:Growth Substances / Q000096:biosynthesis / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008853:Microscopy; D009538:Nicotine / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism
D006133:Growth Substances; D011506:Proteins; D009538:Nicotine