Ultrastructural localization of HMB-45 binding sites.
Three malignant melanomas, two melanoma metastases, two junctional dysplastic nevi, and normal skin were embedded in Lowicryl. Ultrathin sections were incubated with HMB-45 and a gold-labeled anti-mouse antibody. Gold particles indicating the presence of HMB-45 were found in melanosomes Stage 1 and 2 and in the non-melanized portion of melanosomes Stage 3. Melanosomes Stage 4 and melanosome complexes in keratinocytes, as well as in melanophages, were consistently negative. No specific labelling with HMB-45 was seen in eccrine glands of normal skin.
Schaumburg-Lever
G
G
Department of Dermatology, Eberhard-Karls University, Tubingen, Germany.
Metzler
G
G
Kaiserling
E
E
D000911:Antibodies, Monoclonal / Q000032:analysis*; D001666:Binding Sites, Antibody / Q000648:ultrastructure*; D004416:Dysplastic Nevus Syndrome / Q000276:immunology / Q000473:pathology; D006046:Gold; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008543:Melanins / Q000032:analysis; D008544:Melanocytes / Q000276:immunology* / Q000648:ultrastructure*; D008545:Melanoma / Q000276:immunology / Q000473:pathology; D008854:Microscopy, Electron; D015388:Organelles / Q000276:immunology / Q000648:ultrastructure; D012867:Skin / Q000276:immunology / Q000473:pathology / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D008543:Melanins; D006046:Gold