title: Development of an acid hydrolysis method with high recoveries of tryptophan and cysteine for microquantities of protein.
abstract: High recoveries of tryptophan and cysteine were achieved by 12.5 min of hydrolysis with mercaptoethanesulfonic acid vapor. Proteins (1-100 micrograms) were modified by vapor-phase S-pyridylethylation before hydrolysis. The modified proteins were hydrolyzed with the vapor of 2.5 M mercaptoethanesulfonic acid at 176 degrees C. This method promoted efficient hydrolysis of the peptide bonds in proteins and resulted in high recoveries of both tryptophan and cysteine, of 90% or greater, in addition to the other amino acids.
authors.affiliation: Research Institute for Biosciences, Science University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamada
authors.forename: H
authors.initials: H
authors.lastname: Moriya
authors.forename: A
authors.initials: A
authors.lastname: Tsugita
mesh_terms: D000085:Acetates; D019342:Acetic Acid; D000596:Amino Acids / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D003545:Cysteine / Q000032:analysis; D006868:Hydrolysis; D008832:Microchemistry / Q000379:methods*; D009113:Muramidase / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry; D009555:Ninhydrin; D011506:Proteins / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry; D013997:Time Factors; D014364:Tryptophan / Q000032:analysis
chemical_list: D000085:Acetates; D000596:Amino Acids; D009211:Myoglobin; D011506:Proteins; D011725:Pyridines; D014364:Tryptophan; D009113:Muramidase; D009555:Ninhydrin; C029351:4-vinylpyridine; D003545:Cysteine; D019342:Acetic Acid