An in situ preparation of rat trachea for studying the effects of mucosally applied anti-asthma drugs on mediator-induced plasma exudation.
An animal model is described for testing the effects of anti-asthma drugs on mediator-induced airway inflammation. An in situ segment of trachea is slowly perfused with normal saline in spontaneously breathing, anaesthetised rats. Both the anti-asthma drug and the inflammatory mediator can be applied directly to the airway mucosal surface at the same and/or different times. The amount of plasma in samples of perfusate is monitored. Application of a mediator, such as bradykinin, results in an increase in the amount of plasma in the tracheal lumen (and hence in the perfusate). Plasma in the perfusate can be estimated without injecting a labelled tracer for plasma exudation by using a protein native fluorescence (PNF) method as described by Miller-Larsson & Brattsand (3).
O'Donnell
S R
SR
Department of Physiology and Pharmacology, University of Queensland, Brisbane, Australia.
Miller-Larsson
A
A
Brattsand
R
R
Rauchle
K L
KL
D000287:Administration, Topical; D000818:Animals; D001249:Asthma / Q000188:drug therapy*; D001920:Bradykinin / Q000494:pharmacology*; D002199:Capillary Permeability; D016650:Fluorescein-5-isothiocyanate; D005856:Germ-Free Life; D009092:Mucous Membrane; D010949:Plasma / Q000378:metabolism*; D051381:Rats; D014132:Trachea / Q000187:drug effects / Q000378:metabolism*
D016650:Fluorescein-5-isothiocyanate; D001920:Bradykinin