[Effect of oxaceprol on the structure of proteoglycans synthesized by articular chondrocytes from calves].
Calf articular cartilage fragments were incubated in vitro in the absence and presence of 170 micrograms N-acethyl-trans-4, hydroxyproline (oxaceprol) and 20 muCi [35S]-Na2SO4 per ml of culture medium. Newly synthesized 35S labeled proteoglycans (35S-PGs) were extracted with buffered 4M guanidinium chloride (GdmCl) solvent and then characterized with respect to their hydrodynamic sizes, capacity to interact and form aggregates with hyaluronic acid (AH) and the length and composition of their glycosaminoglycan (GAG) side chains. It was demonstrated that extracted 35S-PGs synthesized in the presence of the oxaceprol are not significantly different from the molecules synthesized in the absence of this compound.
Riera
H
H
U18 INSERM, Hôpital Lariboisière, Paris.
Aprile
F
F
Mitrovic
D
D
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism*; D002417:Cattle; D002850:Chromatography, Gel; D004588:Electrophoresis, Cellulose Acetate; D006909:Hydroxyproline / Q000031:analogs & derivatives* / Q000494:pharmacology; D066298:In Vitro Techniques; D011509:Proteoglycans / Q000096:biosynthesis / Q000737:chemistry* / Q000187:drug effects
D000894:Anti-Inflammatory Agents, Non-Steroidal; D011509:Proteoglycans; C012393:oxaceprol; D006909:Hydroxyproline
Effet de l'oxacéprol sur la structure des protéoglycannes synthétisés par les chondrocytes articulaires de veau.