title: A cell-free culture system for development of large numbers of Brugia pahangi larvae.
abstract: Techniques used in the in vitro culture of massive numbers of Brugia pahangi third-stage larvae (L3) are described. Procedures for larval preparation and four culture conditions, with or without animal cell co-cultures, were studied, resulting in the adoption of a relatively simple cell-free culture system for routine harvesting of larval moulting excretory/secretory product.
authors.affiliation: Department of Veterinary Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Hong
authors.forename: M M
authors.initials: MM
authors.lastname: Wong
mesh_terms: D000818:Animals; D002009:Brugia / Q000254:growth & development*; D003470:Culture Media; D007814:Larva / Q000254:growth & development
chemical_list: D003470:Culture Media