Alcohol-induced oxidative stress in rat liver.
1. Livers from rats treated acutely with ethanol showed increased chemiluminescence, malondialdehyde production, and diene formation. Previous administration of (+)-cyanidanol-3 completely abolished acute ethanol-induced chemiluminescence. 2. Rats fed alcohol liquid diets for 3 weeks showed significant increases in microsomal and mitochondrial malondialdehyde formation, and in microsomal H2O2 and O2-. generation. 3. Rats fed a solid basal diet plus ethanol solution for 12 weeks also showed increased microsomal production of O2-. and increased content of microsomal cytochrome P-450. Hydroperoxide-induced chemiluminescence was higher in homogenates, mitochondria and microsomes from ethanol-treated rats than from controls. Vitamins E and A were more effective inhibitors of hydroperoxide-stimulated chemiluminescence in liver homogenates from ethanol-treated rats than from control animals. 4. Results are consistent with peroxidative stress leading to increased lipid peroxidation in liver of rats fed ethanol both acutely and after long-term dosing.
Koch
O R
OR
Departamento de Patologia, Facultad de Medicina, Universidad De Buenos Aires, Argentina.
Galeotti
T
T
Bartoli
G M
GM
Boveris
A
A
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002392:Catechin / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol / Q000633:toxicity*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D008163:Luminescent Measurements; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D013312:Stress, Physiological / Q000139:chemically induced / Q000378:metabolism*; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D000431:Ethanol; D008315:Malondialdehyde; D002392:Catechin; D003577:Cytochrome P-450 Enzyme System; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase