Analysis of the interaction of reserpine with actin by the photoaffinity labelling method.
The interaction of reserpine with one of the cytoskeletal proteins, actin, was analyzed by the photoaffinity labelling method using [3H]reserpine. Reserpine bound sufficiently to G- or oligomeric actin, but hardly to F-actin under the same experimental conditions. This result could be explained if reserpine binds to a specific region of the G-actin molecule that is responsible for actin-actin interactions. It is concordant with this idea that [3H]reserpine bound only to specific proteolytic fragments of actin. When reserpine was mixed with crude extracts of two kinds of tissues, chicken gizzard smooth muscle and bovine adrenal medulla, it bound to the 42 kDa protein of sodium dodecyl sulfate-polyacrylamide gel electrophoresis in both cases. Its molecular size and abundance suggest strongly that this 42 kDa protein is actin. The binding of reserpine to actin was inhibited by dopamine in a dose-dependent manner. These results suggest that actin could be one of the target molecules of reserpine.
Ohmi
K
K
Department of Pharmacology, Faculty of Medicine, University of Tokyo, Japan.
Nakamura
S
S
D000199:Actins / Q000378:metabolism / Q000494:pharmacology*; D000313:Adrenal Medulla / Q000378:metabolism; D000345:Affinity Labels; D000818:Animals; D002417:Cattle; D002645:Chickens; D004298:Dopamine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005895:Gizzard, Avian / Q000378:metabolism; D009130:Muscle, Smooth / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D012110:Reserpine / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D014020:Tissue Extracts / Q000378:metabolism
D000199:Actins; D000345:Affinity Labels; D010446:Peptide Fragments; D014020:Tissue Extracts; D012110:Reserpine; D004298:Dopamine