Cytologic assessment of tumor cell kinetics: applications of monoclonal antibody Ki-67 to fine-needle aspiration smears.
Rates of tumor-cell proliferation often provide prognostic information about a given neoplasm. Previously available methods for accessing cell kinetics are time consuming and expensive, and often require special equipment or radioactive reagents. Monoclonal antibody Ki-67 binds a nuclear antigen expressed in proliferating but not in resting cells. We studied Ki-67 immunostaining of fine-needle aspiration smears from 40 benign and malignant masses. Labeling indices ranged from 0 (thyroid follicular adenoma) to 75 percent (pulmonary oat-cell carcinoma). Frozen section immunostaining (11 cases) and flow cytometric assessment of cell proliferation (8 cases) were in good agreement with Ki-67 labeling indices on smear material. We suggest that this method provides a rapid, inexpensive, and dependable means of assessing tumor-cell kinetics in cytologic preparations.
Henry
M J
MJ
Department of Pathology, Hennepin County Medical Center, Minneapolis, Minnesota 55415.
Stanley
M W
MW
Swenson
B
B
Connolly
J E
JE
Bates
H
H
D000911:Antibodies, Monoclonal; D001707:Biopsy, Needle; D002453:Cell Cycle; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins / Q000032:analysis*; D009369:Neoplasms / Q000737:chemistry* / Q000473:pathology*; D009687:Nuclear Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins