Influence of subchronic exposure to low dietary deoxynivalenol, a trichothecene mycotoxin, on intestinal absorption of nutrients in mice.
During a 6-wk feeding trial, effects of low dietary deoxynivalenol (DON; 0, 0.1, 1 and 10 ppm) on food consumption and weight gain were investigated in male mice. Food intake was similar in all four dietary groups. Weight gain in the group receiving 10 ppm DON was significantly (P less than 0.01) reduced. At the end of the feeding period, test animals were sacrificed and absorption of water, D-glucose, L-leucine, L-tryptophan, 5-methyltetrahydrofolic acid and iron was measured in isolated perfused jejunal segments in vitro. No effects were observed on absorption of water, leucine, tryptophan and iron. However, at a dietary DON concentration of 10 ppm, a slightly but significantly (P less than 0.05) reduced transfer of glucose was measured. Furthermore, transfer as well as tissue accumulation of 5-methyltetrahydrofolic acid in the jejunal segment were both significantly decreased up to 50%. Heavy metal and trace element content was determined in liver, kidney and small intestine. Manganese and molybdenum content in liver tissue was reduced with a DON concentration of 10 ppm in the diet. The findings indicate that subchronic ingestion of DON, in concentrations occurring in contaminated food and feed, results in an impairment of intestinal transfer and uptake of nutrients such as glucose and 5-methyltetrahydrofolic acid.
Hunder
G
G
Walther Straub-Institut für Pharmakologie und Toxikologie, Universität München, Germany.
Schümann
K
K
Strugala
G
G
Gropp
J
J
Fichtl
B
B
Forth
W
W
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D005947:Glucose / Q000378:metabolism; D007408:Intestinal Absorption / Q000187:drug effects*; D007501:Iron / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D008297:Male; D051379:Mice; D013763:Tetrahydrofolates / Q000378:metabolism; D014255:Trichothecenes / Q000633:toxicity*; D014364:Tryptophan / Q000378:metabolism
D013763:Tetrahydrofolates; D014255:Trichothecenes; D014364:Tryptophan; D007501:Iron; D007930:Leucine; D005947:Glucose; C007262:deoxynivalenol; C005984:5-methyltetrahydrofolate