title: Structure and variability of mammalian peroxisomal membrane proteins.
abstract: Peroxisomal membrane proteins (PMPs) from the Swiss-Webster mouse are analyzed and compared to those of rats and humans using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. A purification procedure for fresh mouse, rat, or human biopsy liver which enriches peroxisomal/mitochondrial marker enzyme ratios over 100-fold is characterized. When analyzed by SDS-PAGE, membranes of purified liver peroxisomes are shown to contain the same complement of 145-, 70-, 55-, 36-, and 22-kDa PMPs in rats, mice, and humans. A rabbit polyclonal antibody raised against mouse peroxisomal membranes demonstrates immunoreactivity to 145- and 70-kDa proteins in fresh liver homogenates from all three species and in control or Zellweger syndrome fibroblasts from humans. Human autopsy or placental tissues which were refrigerated before analysis exhibited 105-, 55-, and 36-kDa peptides which may be derived from the 145- and 70-kDa peptides. Such conversions, if related to degradation, may explain difficulties in purifying peroxisomes from human autopsy specimens. Variable amounts of the 55-kDa peptide also occurred in mouse adrenal and lung, and the conversion of higher to lower molecular weight PMPs could not be demonstrated by in vitro incubation of mouse liver. Further definition of the structure and variability of mammalian PMPs should be helpful in understanding polyenzymopathies such as Zellweger syndrome.
authors.affiliation: Department of Pediatrics, Univeristy of Texas Southwestern Medical School, Dallas, 75235.
authors.forename: G N
authors.initials: GN
authors.lastname: Wilson
authors.forename: T E
authors.initials: TE
authors.lastname: King
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D008322:Mammals / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry*; D051379:Mice; D008830:Microbodies / Q000737:chemistry*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D008565:Membrane Proteins