Biological functions of recombinant human interleukin-2.
Recombinant interleukin-2 (rIL-2) is able to maintain the growth and multiplication of T-lymphocytes and IL-2 dependent CTLL-2 cells up to 28 days with the multiplication of T-lymphocytes as high as about 1000 fold. When rIL-2 and its monoclonal antibody were added together, the above functions of rIL-2 were specially blocked, showing that these biological functions were exerted specifically by rIL-2. The rIL-2 is also able to increase the natural killer (NK) activity up to 46-96%, while higher concentrations of rIL-2 might exhibit inhibitory effect. The cytotoxicity of lymphokine activated killer (LAK) cells induced by rIL-2 for killing HL-60 cell line and solid tumour (human lung carcinoma) reached 54.84% and 37.96%, respectively. All these results indicate that the biological functions of rIL-2 were quite similar to that of the natural one.
Liu
X Y
XY
Shanghai Institute of Biochemistry, Chinese Academy of Sciences.
Yu
J L
JL
Zheng
H D
HD
Chen
Y
Y
Shen
J F
JF
Feng
Z H
ZH
Chen
Z C
ZC
You
L F
LF
Sun
B H
BH
Zhou
Y Y
YY
D000911:Antibodies, Monoclonal; D002455:Cell Division; D002478:Cells, Cultured; D006801:Humans; D007376:Interleukin-2 / Q000502:physiology*; D015979:Killer Cells, Lymphokine-Activated / Q000502:physiology; D007694:Killer Cells, Natural / Q000502:physiology; D011994:Recombinant Proteins / Q000502:physiology; D013601:T-Lymphocytes / Q000502:physiology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D011994:Recombinant Proteins