Structure, expression and chromosomal location of the Oct-4 gene.
The map position of Oct-4 on mouse chromosome 17 is between Q and T regions in the Major Histocompatibility Complex (MHC), and it is physically located within 35 kb of a class I gene. Several Oct-4-related genes are present in the murine genome; one of them maps to chromosome 9. The genomic structure and sequence of Oct-4 determined in t-haplotypes reveals five exons, and shows no significant changes in the t12 mutant haplotype making it unlikely that Oct-4 and the t12 early embryonic lethal are the same gene. By in situ hybridization, detectable onset of zygotic Oct-4 expression does not occur until compaction begins at 8-cells, suggesting that there might be other regulatory factors responsible for initiating Oct-4 expression.
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Department of Zoology, University of Texas, Austin.
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Schöler
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D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids; D004247:DNA; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000648:ultrastructure; D016521:Electrophoresis, Gel, Pulsed-Field; D015870:Gene Expression; D008285:Major Histocompatibility Complex; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D005810:Multigene Family; D050814:Octamer Transcription Factor-3; D016133:Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D015053:Zygote / Q000378:metabolism
D004268:DNA-Binding Proteins; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D014157:Transcription Factors; D004247:DNA