title: Complementation of the inability of Lactobacillus strains to utilize D-xylose with D-xylose catabolism-encoding genes of Lactobacillus pentosus.
abstract: The inability of two Lactobacillus strains to ferment D-xylose was complemented by the introduction of Lactobacillus pentosus genes encoding D-xylose isomerase, D-xylulose kinase, and a D-xylose catabolism regulatory protein. This result opens the possibility of using D-xylose fermentation as a food-grade selection marker for Lactobacillus spp.
authors.affiliation: TNO Medical Biological Laboratory, Rijswijk, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Posno
authors.forename: P T
authors.initials: PT
authors.lastname: Heuvelmans
authors.forename: M J
authors.initials: MJ
authors.lastname: van Giezen
authors.forename: B C
authors.initials: BC
authors.lastname: Lokman
authors.forename: R J
authors.initials: RJ
authors.lastname: Leer
authors.forename: P H
authors.initials: PH
authors.lastname: Pouwels
mesh_terms: D019747:Aldose-Ketose Isomerases; D002238:Carbohydrate Epimerases / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D007778:Lactobacillus / Q000235:genetics* / Q000254:growth & development; D014994:Xylose / Q000235:genetics / Q000378:metabolism*
chemical_list: D014994:Xylose; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase