title: Comparative mapping of a gorilla-derived alpha satellite DNA clone on great ape and human chromosomes.
abstract: We have isolated an alpha satellite DNA clone, pG3.9, from gorilla DNA. Fluorescence in situ hybridization on banded chromosomes under high stringency conditions revealed that pG3.9 identifies homologous sequences at the centromeric region of ten gorilla chromosomes, and, with few exceptions, also recognizes the homologous chromosomes in human. A pG3.9-like alphoid DNA is present on a larger number of orangutan chromosomes, but, in contrast, is present on only two chromosomes in the chimpanzee. These results show that the chromosomal subsets of related alpha satellite DNA sequences may undergo different patterns of evolution.
authors.affiliation: Department of Human Genetics, Yale University School of Medicine, New Haven, CT 06510.
authors.forename: A
authors.initials: A
authors.lastname: Baldini
authors.forename: D A
authors.initials: DA
authors.lastname: Miller
authors.forename: V
authors.initials: V
authors.lastname: Shridhar
authors.forename: M
authors.initials: M
authors.lastname: Rocchi
authors.forename: O J
authors.initials: OJ
authors.lastname: Miller
authors.forename: D C
authors.initials: DC
authors.lastname: Ward
mesh_terms: D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002871:Chromosome Banding; D002874:Chromosome Mapping; D004276:DNA, Satellite / Q000235:genetics*; D006071:Gorilla gorilla; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D002679:Pan troglodytes; D011148:Pongo pygmaeus; D011323:Primates / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D004276:DNA, Satellite