[Development of integrative vectors for Actinomycetes--producers of antibiotics].
The integrative vectors pSU 475 and pSU 476 with variable numbers of copies per genome were developed for antibiotic producing actinomycetes. For this, the amplifying sequence AUD-Sr 1 of Streptomyces rimosus and the BamHIB fragment of the eSA 1 genetic element from Streptomyces antibioticus were used. The eSA 1 fragment was an element required for integration of a vector to the actinomycete chromosomes since it was homologous with the chromosomal DNAs of S. lividans, S. erythraeus and S. antibioticus. At the first stage the AUD-Sr 1 sequence within the actinomycete plastid pSU 23 was cloned by the vector pUC 19 to E coli. In that experiment the 12.4-kb plasmid pSU 449 was isolated. At the second stage the BamHIB-fragment of the eSA 1 element was incorporated into the resultant hybrid plasmid pSU 449. The 16.5-kb hybrid plasmids pSU 475 and pSU 476 were isolated. In these plasmids the BamHIB fragment of eSA 1 was present in two orientations. The developed vectors were useful in cloning DNA to S. lividans and S. erythraeus.
Grigor'ev
A E
AE
Zhukov
D B
DB
Orlova
V A
VA
Danilenko
V N
VN
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005784:Gene Amplification / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
D000900:Anti-Bacterial Agents
Sozdanie integrativnykh vektorov dlia aktinomitsetov--produtsentov antibiotikov.