Cloning and nucleotide sequencing of the gene encoding the beta-lactamase from Citrobacter diversus.
The gene coding for the class A beta-lactamase of Citrobacter diversus has been cloned and sequenced. It contains the information for a 294-amino-acid precursor protein, including a 27-residue N-terminal signal peptide. The deduced sequence of the N-terminal portion of the mature protein is in excellent agreement with that determined by microsequencing of the protein and readily explains the pI differences observed between the naturally occurring forms I and II of the enzyme. The sequence of the mature protein exhibits a very high degree of similarity with that of the Klebsiella oxytoca class A beta-lactamase.
Perilli
M
M
Department of Biomedical Sciences and Technology and Biometry, Località Collemaggio, L'Aquila, Italy.
Franceschini
N
N
Segatore
B
B
Amicosante
G
G
Oratore
A
A
Duez
C
C
Joris
B
B
Frère
J M
JM
D000595:Amino Acid Sequence; D001483:Base Sequence; D002954:Citrobacter / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D012689:Sequence Homology, Nucleic Acid; D001618:beta-Lactamases / Q000235:genetics*
D009838:Oligodeoxyribonucleotides; D001618:beta-Lactamases