Purification and properties of growth inhibitor from normal rabbit serum.
It was previously found that rabbit serum contains a growth-inhibitory substance for a tumorigenic rat liver cell line RSV-BRL. In the present study, the growth inhibitor was purified from normal rabbit serum to show a homogeneous protein band with a molecular weight (Mr) of 56 k on SDS-polyacrylamide gel electrophoresis under non-reducing conditions. The purified growth inhibitor, tentatively named rabbit serum-derived growth inhibitor (RSGI), potently inhibited the growth of RSV-BRL and nine kinds of other cell lines including three human tumor cell lines at a concentration of 20 ng/ml or higher. The growth-inhibitory effect of RSGI was reversible and appeared to be cytostatic rather than cytotoxic. RSGI was stable to heating at 56 degrees C for 30 min or treatment with 0.1 M 2-mercaptoethanol, but labile to heating at 100 degrees C for 3 min or treatment with 1 M acetic acid (pH 2.3), 6 M urea, 50% (v/v) 1-propanol, or 0.1% (w/v) trypsin. These properties of RSGI suggested that it was different from type beta transforming growth factors, tumor necrosis factor-alpha, and other known growth-regulatory factors.
Kimura
T
T
Division of Enzymology, Osaka University.
Miyazaki
K
K
Mashima
K
K
Yamashita
J
J
Horio
T
T
Kakuno
T
T
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002845:Chromatography; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003470:Culture Media; D006131:Growth Inhibitors / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D006860:Hydrogen Bonding; D008970:Molecular Weight; D011817:Rabbits; D051381:Rats
D003470:Culture Media; D006131:Growth Inhibitors