Fluorometric assay of microsomal aldehyde oxygenase activity: 9-anthraldehyde as a substrate.
With regard to hepatic microsomal oxidation of 9-anthraldehyde (9-AA), a fluorometric method for determination of 9-anthracene carboxylic acid (9-ACA) is described. 9-AA was incubated with hepatic microsomes prepared from male ddN mice. 9-ACA formed was fluorometrically (excitation and emission wavelengths of 255 and 458 nm, respectively) quantitated after the separation from 9-AA by an alkali extraction and ethyl acetate reextraction. Hepatic microsomes less than 0.1 mg protein were enough to assay the microsomal aldehyde oxidation. The enzyme in the microsomes that catalyzes the oxidation of 9-AA to 9-ACA has been characterized by this method.
Watanabe
K
K
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
Ozaki
M
M
Matsunaga
T
T
Yamamoto
I
I
Yoshimura
H
H
D000445:Aldehyde Oxidoreductases / Q000032:analysis* / Q000737:chemistry; D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D000873:Anthracenes / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology*; D010105:Oxygenases / Q000032:analysis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods
D000447:Aldehydes; D000873:Anthracenes; C073050:9-anthraldehyde; C010030:9-anthroic acid; D010105:Oxygenases; D000445:Aldehyde Oxidoreductases