title: Structure of the O-antigen of Francisella tularensis strain 15.
abstract: The O-specific polysaccharide, obtained by mild acid degradation of the lipopolysaccharide of Francisella tularensis strain 15, contained 2-acetamido-2,6-dideoxy-D-glucose (D-QuiNAc), 4,6-dideoxy-4-formamido-D-glucose (D-Qui4NFm), and 2-acetamido-2-deoxy-D-galacturonamide (D-GalNAcAN) in the ratios 1:1:2. Tri- and tetra-saccharide fragments were obtained on treatment of the polysaccharide with anhydrous hydrogen fluoride and partial hydrolysis with 0.1 M hydrochloric acid, respectively. On the basis of 1H- and 13C-n.m.r. spectroscopy of the polysaccharide and the saccharides, it was concluded that the O-antigen had the structure: ----4)-alpha-D-GalpNAcAN-(1----4)-alpha-D-GalpNAcAN-(1----3) -beta-D-QuipNAc-(1----2)-beta-D-Quip4NFm-(1----. This O-antigen is related in structure to those of Pseudomonas aeruginosa O6, immunotype 1, and IID 1008, and Shigella dysenteriae type 7.
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the U.S.S.R., Moscow.
authors.forename: E V
authors.initials: EV
authors.lastname: Vinogradov
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
authors.forename: N K
authors.initials: NK
authors.lastname: Kochetkov
authors.forename: N V
authors.initials: NV
authors.lastname: Tochtamysheva
authors.forename: S F
authors.initials: SF
authors.lastname: Averin
authors.forename: O V
authors.initials: OV
authors.lastname: Goncharova
authors.forename: V S
authors.initials: VS
authors.lastname: Khlebnikov
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005604:Francisella tularensis / Q000737:chemistry / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000276:immunology; D012762:Shigella dysenteriae / Q000737:chemistry / Q000276:immunology; D013045:Species Specificity
chemical_list: D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial