Steroid hormone specifically binds to rat kidney plasma membrane.
A high-affinity and low-capacity corticosterone specific binding was detected in the purified plasma membrane preparation from rat kidney using an in vitro steroid hormone binding assay. The specific-bound hormone was efficiently distinguished from the irreversible-bound hormone with 10 microM corticosterone. Under standardized conditions of pH 7.4 at 2 degrees C and 30 min incubation time, the binding was saturable and showed Kd = 13 +/- 3 nM and Bmax = 616 +/- 34 fmol/mg of protein. Competitive binding studies with analogue steroids indicated that corticosterone binding to kidney plasma membrane is hormone-specific. Results indicated that the possible nongenomic effects of steroids could be mediated by their interaction with plasma membrane.
Ibarrola
I
I
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad del País Vasco/Euskal Herriko Unibersitatea, Bilbao, Spain.
Ogiza
K
K
Marino
A
A
Macarulla
J M
JM
Trueba
M
M
D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D003345:Corticosterone / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D013256:Steroids / Q000378:metabolism
D013256:Steroids; D003345:Corticosterone