title: Effect of ferric and ferrous iron chelators on growth of Bacteroides fragilis under anaerobic conditions.
abstract: Growth of Bacteroides fragilis under anaerobic conditions in the presence of either haemin or protoporphyrin IX was inhibited by the ferrous iron chelator bipyridyl. The ferric-iron chelator desferrioxamine inhibited growth in the presence of protoporphyrin but not haemin, suggesting that even under anaerobic conditions Fe3+ is involved in uptake of non-haem iron, which is required in the absence of haemin. However, the ferric iron chelators 1,2-dimethyl-3-hydroxy-pyrid-4-one (L1) and pyridoxal isonicotinoyl hydrazone (PIH) were only weakly inhibitory. Apotransferrin, which also binds Fe3+, inhibited growth, but this was not simply due to binding of iron in the medium, as under the reducing conditions present, transferrin was unable to bind iron. This study suggests that even under anaerobic conditions, uptake of non-haem iron by B. fragilis may involve conversion of Fe2+ to Fe3+.
authors.affiliation: University Department of Immunology, Western Infirmary, Glasgow, Scotland.
authors.forename: E R
authors.initials: ER
authors.lastname: Rocha
authors.forename: M
authors.initials: M
authors.lastname: de Uzeda
authors.forename: J H
authors.initials: JH
authors.lastname: Brock
mesh_terms: D000693:Anaerobiosis; D001441:Bacteroides fragilis / Q000187:drug effects* / Q000254:growth & development; D005290:Ferric Compounds / Q000494:pharmacology*; D005296:Ferrous Compounds / Q000494:pharmacology*; D006427:Hemin / Q000494:pharmacology; D007501:Iron / Q000494:pharmacology; D007502:Iron Chelating Agents / Q000494:pharmacology*; D007700:Kinetics; D010084:Oxidation-Reduction; D011524:Protoporphyrins / Q000494:pharmacology; D014168:Transferrin / Q000494:pharmacology
chemical_list: D005290:Ferric Compounds; D005296:Ferrous Compounds; D007502:Iron Chelating Agents; D011524:Protoporphyrins; D014168:Transferrin; D006427:Hemin; D007501:Iron