An in-vitro direct chemiluminescence assay for assessment of platelet-bound antibody in thrombocytopenic patients.
A direct chemiluminescence test (CLT) employing autologous monocytes and granulocytes was used to assay platelet-bound antibody in comparison to the direct platelet suspension immunofluorescence test (PSIFT) in 50 patients. Twenty-six of the 30 immune thrombocytopenic purpura (ITP) patients, including all 21 chronic ITP patients with platelet counts below 100 x 10(9)/l, showed positive results with at least one type of phagocytic cell; so did seven of the eight patients with other presumed autoimmune thrombocytopenias, and three out of 12 patients with thrombocytopenia associated with non-immune disorders. This test appears potentially valuable in the clinical diagnosis of immune thrombocytopenias.
Kazemi
A
A
Division of Haematology, United Medical School of Guy's Hospital, London.
Singh
A K
AK
Slater
N G
NG
D001323:Autoantibodies / Q000032:analysis*; D001327:Autoimmune Diseases / Q000175:diagnosis; D001792:Blood Platelets / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008163:Luminescent Measurements; D010587:Phagocytosis; D013921:Thrombocytopenia / Q000175:diagnosis / Q000276:immunology*
D001323:Autoantibodies