Further characterisation of the Schistosoma japonicum protein Sj23, a target antigen of an immunodiagnostic monoclonal antibody.
Sj23, the 23-kDa target antigen in Schistosoma japonicum adult worms of the hybridoma monoclonal antibody (mAb) I-134, has been identified and cloned from cDNA libraries, mAb I-134 has been successfully used in immunodiagnostic assays to detect S. japonicum infection in Philippine patients. Sequence analysis has shown that Sj23 is the homologue, with 84% amino acid identity, of Sm23, a 23-kDa molecule from S. mansoni worms previously described from our laboratory. The domain structures of Sj23 and Sm23 are strikingly similar to the human membrane proteins ME491, CD37, CD53 and TAPA-1, which may suggest a functional role for the schistosome molecules in cellular proliferation.
Davern
K M
KM
Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
Wright
M D
MD
Herrmann
V R
VR
Mitchell
G F
GF
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015801:Helminth Proteins; D006801:Humans; D007159:Immunologic Tests; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D012549:Schistosoma japonicum / Q000235:genetics / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000175:diagnosis; D012689:Sequence Homology, Nucleic Acid
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D015801:Helminth Proteins; D008565:Membrane Proteins; C072633:Sj23 antigen, Schistosoma japonicum; D004247:DNA