title: Monitoring of factor XII activity and granulocyte elastase release during cardiopulmonary bypass.
abstract: Monitoring of the influence of cardiopulmonary bypass (CPB) on hematologic parameters is relevant to an improved understanding of the pathophysiology produced, as well as for the development of improved methods. The selection of suitable parameters is highly important. In this study, both contact phase activation and leukocyte response have been studied. Contact activation was determined by a novel assay for the measurement of Factor XII activity (FXIIA), and leukocyte response was measured by the release of granulocyte elastase. Five patients undergoing elective coronary artery surgery using a bubble oxygenator and pulsatile perfusion were studied. A notable feature of this study was the gradual increase of FXIIA during the study period, with granulocyte elastase levels following a similar pattern. Both FXIIA and granulocyte elastase are appropriate parameters for monitoring CPB, and could be useful in studying alternative bypass procedures and antithrombotic agents.
authors.affiliation: Bioengineering Unit, University of Strathclyde, Glasgow, Scotland, United Kingdom.
authors.forename: L
authors.initials: L
authors.lastname: Irvine
authors.forename: S
authors.initials: S
authors.lastname: Sundaram
authors.forename: J M
authors.initials: JM
authors.lastname: Courtney
authors.forename: D P
authors.initials: DP
authors.lastname: Taggart
authors.forename: D J
authors.initials: DJ
authors.lastname: Wheatley
authors.forename: G D
authors.initials: GD
authors.lastname: Lowe
mesh_terms: D000368:Aged; D001672:Biocompatible Materials; D002315:Cardiopulmonary Bypass; D001026:Coronary Artery Bypass; D005174:Factor XII / Q000378:metabolism*; D005260:Female; D006801:Humans; D019272:Leukocyte Elastase; D008213:Lymphocyte Activation / Q000502:physiology; D008297:Male; D008875:Middle Aged; D016343:Monitoring, Intraoperative / Q000379:methods; D010106:Oxygenators; D010196:Pancreatic Elastase / Q000378:metabolism*
chemical_list: D001672:Biocompatible Materials; D005174:Factor XII; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase