[Enhancing effect of protein kinase C activation on high K(+)-induced norepinephrine release].
Raising the concentration of K+ in the superfusion medium increased the release of [3H]norepinephrine ([3H]NE) from rabbit hippocampus slices preincubated with [3H]NE. This evoked [3H]NE release was modulated by presynaptic alpha 2-adrenergic, presynaptic opioid kappa- and A1-adenosine receptors and augmented with increasing K+ concentration (20-120 mmol.L-1). The enhancement or inhibition of this release by activator (phorbol ester) or inhibitor (polymyxin B) of protein kinase C was blocked by tetrodotoxin and reduced with increasing K+ concentration. It was suggested that the Na(+)-influx was involved in the protein kinase C effect on the K(+)-evoked [3H]NE release.
Huang
H Y
HY
Shanghai Institute of Physiology, Chinese Academy of Sciences.
Dai
S P
SP
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006624:Hippocampus / Q000378:metabolism*; D066298:In Vitro Techniques; D009638:Norepinephrine / Q000378:metabolism*; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D011112:Polymyxin B / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology*; D011493:Protein Kinase C / Q000494:pharmacology*; D011817:Rabbits; D013779:Tetrodotoxin / Q000494:pharmacology
D015240:Phorbol 12,13-Dibutyrate; D013779:Tetrodotoxin; D011493:Protein Kinase C; D011112:Polymyxin B; D011188:Potassium; D009638:Norepinephrine