Mn[III] uroporphyrin I: a novel metalloporphyrin contrast agent for magnetic resonance imaging.
We have used an intracranial 9L rat brain tumor model to determine whether a novel metalloporphyrin, Mn[III] uroporphyrin I (MnUROP-I), could function as an intravenous MRI contrast agent for brain tumors. In several experiments, 24 male Fischer 344 rats were inoculated intracranially with 9L brain tumor cells. On day 15 postinoculation, animals were anesthetized and the femoral vein exposed. Prior to the intravenous injection of the contrast agent, a precontrast scan (1 Tesla in a standard head coil) was performed. Thirty min after injection of the contrast agent, a postcontrast scan was performed. Although there was only a suggestion of abnormality on the precontrast scans, the presence of tumor was visibility enhanced in the postcontrast scans. In 3 animals scanned at 24 hr postinjection, persistent tumor enhancement was demonstrated. Measured tumor sizes on the MRI scans were consistent with sizes measured at autopsy and histologically. These results demonstrate that MnUROP-I is an effective MRI contrast agent for the detection of an intracranial brain tumor in the rat model.
McMillan
J H
JH
Department of Diagnostic Radiology, University of Kansas Medical Center, Kansas City 66103.
Cox
G G
GG
Kimler
B F
BF
Spicer
J S
JS
Batnitzky
S
S
D000818:Animals; D001932:Brain Neoplasms / Q000175:diagnosis / Q000473:pathology; D003287:Contrast Media / Q000008:administration & dosage / Q000032:analysis; D004355:Drug Stability; D005910:Glioma / Q000175:diagnosis / Q000473:pathology; D007089:Image Enhancement / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008345:Manganese / Q000008:administration & dosage / Q000032:analysis; D008665:Metalloporphyrins / Q000008:administration & dosage / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D013053:Spectrophotometry
D003287:Contrast Media; D008665:Metalloporphyrins; C072706:Mn(III) uroporphyrin I; D008345:Manganese