The biological function of hementin in the proboscis of the leech Haementeria ghilianii.
The giant Amazon leech Haementeria ghilianii feeds by inserting an exceedingly long tubular proboscis (up to 10 cm) deep into its mammalian host. The wound from its bite is not associated with prolonged bleeding because all antihaemostatic factors, including the fibrinogenolytic enzyme hementin, appear to be secreted exclusively into the lumen of the proboscis. It is in this narrow lumen that blood first comes into contact with hementin, the secretion of which is under neuronal control from the brain. During feeding, about 15 ml of blood are sucked through the proboscis at the rate of approximately 0.14 ml/min. A complete passage of blood takes less than 1 min, much faster than the approximately 6 min needed for coagulation (fibrin formation). Therefore, it is unlikely that hementin functions in the proboscis simply to prevent fibrin formation. Of greater risk is platelet aggregation which can occur within 1-2 min. The formation of a platelet-rich clot within the proboscis could make the proboscis non-functional. Hementin's unique ability to dissolve platelet-rich clots offers a way of restoring blood flow through the proboscis. Hementin is able to disaggregate platelets by breaking the fibrinogen crosslink between platelets. Hementin's unique cleavage site in the connector region of platelet-bound fibrinogen is thought to be a most effective mechanism for eliminating the crosslinking.
Sawyer
R T
RT
Biopharm, UK, Ltd., Dyfed, Wales.
Jones
C P
CP
Munro
R
R
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D005247:Feeding Behavior / Q000502:physiology; D005340:Fibrinogen / Q000378:metabolism; D005786:Gene Expression Regulation; D007865:Leeches / Q000033:anatomy & histology / Q000201:enzymology / Q000502:physiology*; D008322:Mammals / Q000097:blood; D008666:Metalloendopeptidases / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D009490:Neurosecretory Systems / Q000502:physiology; D012469:Salivary Glands / Q000033:anatomy & histology / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000502:physiology; D012701:Serotonin / Q000502:physiology; D014426:Turtles / Q000097:blood
D012471:Salivary Proteins and Peptides; D012701:Serotonin; D005340:Fibrinogen; D008666:Metalloendopeptidases; C040192:hementin