A study of the beta-lactamases of 100 clinical isolates of Yersinia enterocolitica.
The intracellular beta-lactamases of 100 distinct clinical isolates of Yersinia enterocolitica were examined, to investigate the effect of selected beta-lactam agents on the enzymes. The enzyme inhibition profiles obtained were specific to each of the three biotypes 1A, 3 and 4. Biotype 4 strains elaborated only one beta-lactamase with a pI of 8.3 corresponding to the carbenicillin-hydrolysing beta-lactamase enzyme A of Cornelis & Abraham. Biotype 3 strains also produced a single beta-lactamase with a pI of 5.4-5.6 corresponding to enzyme B, the chromosomally mediated inducible cephalosporinase. Two beta-lactamases were found in biotype 1A strains, one was identical to enzyme A and the other, although showing a slight difference in pI to enzyme B, was similar to this enzyme in other respects. The differences in the distribution of enzyme A and enzyme B in strains of Y. enterocolitica belonging to the biotypes 1A, 3 and 4 isolated in Australia, explain the different patterns of susceptibility to beta-lactam antibiotics.
Pham
J N
JN
Department of Microbiology, Prince of Wales Hospital, Randwick NSW, Australia.
Bell
S M
SM
Lanzarone
J Y
JY
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D004352:Drug Resistance, Microbial / Q000235:genetics; D007525:Isoelectric Focusing; D008826:Microbial Sensitivity Tests; D015008:Yersinia enterocolitica / Q000145:classification / Q000187:drug effects / Q000201:enzymology*; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000096:biosynthesis*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D065093:beta-Lactamase Inhibitors; D047090:beta-Lactams; D001618:beta-Lactamases