Influence of yeasts and of their constituents on nucleoside uptake in peritoneal murine macrophages.
A marked reduction of [3H]-uridine uptake was observed when mouse peritoneal macrophages (pM phi) were exposed to heat-killed Candida albicans or Saccharomyces cerevisiae. By contrast, an increased nucleoside uptake was promoted by yeast products such as zymosan, laminarin, or yeast cell-wall extracts, which are mainly composed of beta-glucans and alpha-mannans. In a search for the active fungal component(s), the uptake process was shown to be differently affected by monosaccharides and polysaccharides. These findings support the view that a specific recognition of a pM phi membrane receptor is mediating the effect of the various substances.
Busolo
F
F
Institute of Microbiology, Padua University, Faculty of Medicine, Italy.
Palù
G
G
Conventi
L
L
D000818:Animals; D002176:Candida albicans / Q000737:chemistry / Q000502:physiology*; D002241:Carbohydrates / Q000494:pharmacology; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D007700:Kinetics; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D010529:Peritoneal Cavity; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000502:physiology*; D014529:Uridine / Q000378:metabolism*
D002241:Carbohydrates; D003470:Culture Media; D014529:Uridine