Chemical modification studies of Artocarpus lakoocha lectin artocarpin.
The effect of chemical modification on an anti T-like lectin, artocarpin isolated from Artocarpus lakoocha seeds was investigated in order to identify the type of amino acids involved in its agglutinating activity. Modification of carboxyl groups, arginine and lysine residues, did not affect the lectin activity. However, modification of tryptophan, tyrosine and histidine residues led to a complete loss of its activity, indicating the involvement of these amino acids in the saccharide-binding ability. A protection was observed in the presence of inhibitory sugar. A marked decrease in the fluorescence emission was found when the tryptophan residues of lectin were modified. The circular dichroism spectra showed the presence of an identical pattern of conformation in the native and modified lectin, indicating that the loss in activity was due to modification only. The effect of pronase on artocarpin showed loss of activity whereas papain and trypsin had no effect. The specific activity of artocarpin remained unaltered on treatment with glycosidases but remarkable increase in the activity (of the same) was observed with xylanase treatment. Immunodiffusion studies with chemically modified lectin showed no gross structural changes, indicating that the group specific modifying agents did not alter the antigenic sites of the modified lectin.
Chowdhury
S
S
Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Calcutta.
Ahmed
H
H
Chatterjee
B P
BP
D000596:Amino Acids / Q000737:chemistry; D002942:Circular Dichroism; D006388:Hemagglutinins / Q000737:chemistry / Q000276:immunology; D007120:Immunochemistry; D037102:Lectins / Q000737:chemistry* / Q000276:immunology; D037241:Mannose-Binding Lectins; D015394:Molecular Structure; D037121:Plant Lectins; D013050:Spectrometry, Fluorescence
D000596:Amino Acids; D006388:Hemagglutinins; D037102:Lectins; D037241:Mannose-Binding Lectins; D037121:Plant Lectins; C090683:artocarpin lectin; C043696:jacalin