[Age-dependent changes in glucocorticoid receptors in rat liver following functional loading in in-vivo and in-vitro systems].
In aging the transformation, nuclei binding, and recycling of glucocorticoid receptor complexes (GRC) seem to be retarded or disturbed, as shown in the rat liver after stress and in an in vitro-system. Reasons of the retarded in vitro-transformation and DNA-binding could be changed properties of the receptor protein or of cytosolic modulators, in which the tested heat-stable factor f is not responsible for the aging changes. The observed aging differences, when studying the nuclei binding after Ca2+ addition, result in the question, to what extent phosphorylation/dephosphorylation processes play a role in transformation and nuclei binding of GRC.
Martin
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R
Institut für Klinische Chemie und Laboratoriumsdiagnostik, Universität Leipzig.
Ulbrich
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I
Martin
H
H
Rotzsch
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W
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000378:metabolism*
D004268:DNA-Binding Proteins; D011965:Receptors, Glucocorticoid; D004247:DNA; D002118:Calcium
Altersabhängige Veränderungen von Glucocorticoidrezeptoren der Rattenleber nach funktioneller Belastung in vivo und im In-vitro-System.