Differential effects of long-term lithium and carbamazepine administration on Gs alpha and Gi alpha protein in rat brain.
The G protein subunits, Gs alpha, Gi alpha, and Go alpha, have been quantitated in various rat brain regions using enzyme-linked immunosorbent assay (ELISA) techniques. Chronic (3-week) treatment with carbamazepine decreased Gs alpha in several brain regions reaching significance in the neostriatum, while chronic lithium treatment had no unequivocal effect. Lithium significantly increased Gi alpha in the hypothalamus and hippocampus, whereas carbamazepine decreased Gi alpha in the frontal cortex. Both treatments had no consistent effects on Go alpha. We conclude that long-term treatment with lithium and carbamazepine exerts differential effects on G protein alpha subunits, and that this modification of signal transduction represents a potential mechanism of antibipolar drug-induced neural plasticity.
Lesch
K P
KP
Laboratory of Clinical Science, National Institute of Mental Health, Bethesda, MD 20892.
Aulakh
C S
CS
Tolliver
T J
TJ
Hill
J L
JL
Wolozin
B L
BL
Murphy
D L
DL
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D002220:Carbamazepine / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D019204:GTP-Binding Proteins / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D008094:Lithium / Q000494:pharmacology*; D046911:Macromolecular Substances; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D015398:Signal Transduction / Q000502:physiology; D013997:Time Factors
D046911:Macromolecular Substances; D002220:Carbamazepine; D008094:Lithium; D019204:GTP-Binding Proteins