title: [Preparation and characterization of monoclonal antibodies to human apolipoprotein E].
abstract: Monoclonal antibodies to human apolipoprotein E (apoE) were prepared and characterized. Antibodies of 3D12F11 clone were shown to be specific to apoE and belong to IgG1 subclass. Dissociation constant of antigen-antibody complex in solution was determined as 3.5 +/- 0.5 nM. The antibodies interacted neither heparin- nor lipid-binding sites of human apoE molecule. The obtained antibodies may be useful for metabolic and structural investigations of apoE as well as for clinical studies.
authors.forename: V V
authors.initials: VV
authors.lastname: Shuvaev
authors.forename: E V
authors.initials: EV
authors.lastname: Ianushevskaia
authors.forename: A D
authors.initials: AD
authors.lastname: Dergunov
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001057:Apolipoproteins E / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D001057:Apolipoproteins E; D007074:Immunoglobulin G
vernacular_title: Poluchenie i kharakteristika monoklonal'nykh antitel protiv apolipoproteina E cheloveka.