Antitumor action of an acidic glycoprotein (SAGP) from Streptococcus pyogenes in mice.
We have shown that an acidic glycoprotein (SAGP) isolated from a cell-free extract of Streptococcus pyogenes (Su strain) prolonged the life-span of Ehrlich ascites carcinoma (EAC)-bearing mice. The present study shows that the life-span prolonging effect of SAGP in EAC-bearing mice was reduced by whole body X-irradiation before EAC inoculation. SAGP (500 micrograms protein/mouse/day X 4, i.p.) also showed a life-span prolonging effect (T/C (%) = 169) on Meth A fibrosarcoma (Meth A)-bearing mice, but the effect of SAGP was abrogated by an i.p. pretreatment of the host with carrageenan, an antimacrophage agent. The spleen cells from the Meth A-inoculated and SAGP-treated mice were found to have a considerable cytostatic activity by a 3H-thymidine incorporation assay. But the activity disappeared in the presence of carrageenan. These results suggest that the in vivo antitumor effects of SAGP are mediated through its immunomodulating action.
Yoshida
J
J
Department of Pharmacology, Kanazawa Medical University, Ishikawa, Japan.
Takamura
S
S
Suzuki
S
S
D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000818:Animals; D001426:Bacterial Proteins / Q000627:therapeutic use*; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology / Q000532:radiotherapy / Q000628:therapy*; D002351:Carrageenan / Q000494:pharmacology; D003131:Combined Modality Therapy; D003602:Cytotoxicity, Immunologic; D005260:Female; D006023:Glycoproteins / Q000627:therapeutic use*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008813:Mice, Inbred ICR / Q000276:immunology; D009368:Neoplasm Transplantation; D012513:Sarcoma, Experimental / Q000628:therapy*; D013154:Spleen / Q000276:immunology; D013297:Streptococcus pyogenes / Q000737:chemistry; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014916:Whole-Body Irradiation
D000276:Adjuvants, Immunologic; D001426:Bacterial Proteins; D006023:Glycoproteins; C045283:SAGP protein, Streptococcus; D002351:Carrageenan