Evidence for kinetic and immunologic heterogeneity of dihydrofolate reductase in L1210 leukemia cells.
Two species of DHFR were identified in wild-type L1210 murine leukemia cells by analysis of the kinetics of the binding of MTX and dissociation of the MTX-enzyme complex at pH 5.0 and pH 7.2. The two forms of DHFR were also distinguished by immunoinhibition of the binding of MTX and the catalytic reduction of FH2 to FH4 using an antiserum raised to the purified high affinity form of DHFR. The Ka for the binding of MTX by the low affinity form of the enzyme is 4.5 x 10(7) M-1, substantially lower than the reported Ka for the binding of this drug by the high affinity enzyme. The low affinity form of the enzyme catalyzed the reduction of FH2 to FH4 at a rate slower than the high affinity form of DHFR.
Iqbal
M P
MP
Department of Biochemistry, Aga Khan University Medical College, Karachi, Pakistan.
Rothenberg
S P
SP
da Costa
M
M
D000818:Animals; D002384:Catalysis; D003600:Cytosol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera / Q000276:immunology; D007527:Isoenzymes / Q000276:immunology*; D007700:Kinetics; D007939:Leukemia L1210 / Q000201:enzymology*; D008727:Methotrexate / Q000378:metabolism*; D013762:Tetrahydrofolate Dehydrogenase / Q000276:immunology*
D007106:Immune Sera; D007527:Isoenzymes; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate