[Dying kinetics of H. influenzae in fibrin clots under the influence of various antibiotics].
Traditional test methods with determination of MIC values alone are not sufficient for the estimation of antimicrobial activity in tissues. Various other parameters like protein binding, the pH value of tissues and other yet unknown parameters influence bacterial killing under the influence of various antibiotics. In an in vitro model using fibrin clots kill kinetics of H. influenzae were determined. Concentrations of antibiotics were determined in fibrin clots. In these in vitro experiments the eradication of H. influenzae from fibrin clots was investigated under the influence of various antibiotics in different concentrations. The results demonstrate, that the relationship between MIC value and tissue concentration is of little value to determine the antimicrobial activity of beta lactam antibiotics and macrolide antibiotics of H. influenzae in fibrin clots. Macrolide antibiotics demonstrate a bactericidal mode of action.
Guggenbichler
J P
JP
Universitätsklinik für Kinder und Jugendliche, Universität Erlangen-Nürnberg, Deutschland.
Allerberger
F
F
D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology*; D005337:Fibrin / Q000737:chemistry; D006193:Haemophilus influenzae / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D007700:Kinetics
D000900:Anti-Bacterial Agents; D005337:Fibrin
Absterbekinetik von H. influenzae in Fibrinklumpen unter dem Einfluss verschiedener Antibiotika.