CUBIC: a three-dimensional colored projection of Consort 30 generated trivariate flow cytometric data.
The CUBIC program displays three-dimensional colored dot plots of flow cytometric trivariate data collected by unmodified commercial instruments (FACScan flow cytometer, FACS 440 cell sorter). Assuming a bimodal distribution of the fluorescence intensity of the cells, the eight theoretical subpopulations involved in a three-color fluorescence histogram are clearly localized in the 3-D space by colored dots that are clustered near each corner of a cubic frame. Rotation, tilting, and zoom functions are available. Table look-up is not needed. CUBIC was illustrated by two experiments: 1) three-color immunofluorescence of antigens on human lymphocytes using monoclonal antibodies conjugated either to fluorescein (FITC), to R-phycoerythrin (PE), or to biotin revealed by a streptavidin coupled to a PE-Texas red tandem conjugate (TC); 2) two-color immunofluorescence of CD4 and CD8 antigens on thymocytes of healthy or preleukemic mice correlated to the DNA content quantified by 7-amino-actinomycin D (7-AAD). The three fluorescences were excited by a single argon-ion laser emitting at 488 nm.
Greimers
R
R
Laboratory of Pathologic Anatomy, University Hospital of Liege, Belgium.
Rongy
A M
AM
Schaaf-Lafontaine
N
N
Boniver
J
J
D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D002469:Cell Separation / Q000295:instrumentation; D003116:Color; D003196:Computer Graphics; D004247:DNA / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis; D003609:Dactinomycin / Q000031:analogs & derivatives; D005434:Flow Cytometry / Q000295:instrumentation; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006801:Humans; D007962:Leukocytes; D008223:Lymphoma / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009381:Neoplasms, Radiation-Induced / Q000473:pathology; D010799:Phycoerythrin; D011289:Preleukemia / Q000473:pathology; D012984:Software; D013953:Thymus Neoplasms / Q000473:pathology
D000943:Antigens, Differentiation; D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm; D005456:Fluorescent Dyes; D010799:Phycoerythrin; D003609:Dactinomycin; C025942:7-aminoactinomycin D; D004247:DNA