title: Catalytic activity and thermostability of dehydrogenase conjugates with cortisol and progesterone.
abstract: The conjugates of glucose-6-phosphate dehydrogenase, lactate dehydrogenase, and malate dehydrogenase with progesterone and cortisol, containing 1-40 steroid molecules per enzyme molecule, were obtained by the reactions of N-succinimide esters of the 3-[O-(carboxymethyl)oximes)] of cortisol and progesterone with a protein in a water-DMFA (10%) medium. The catalytic activity and thermostability of dehydrogenases and their steroid conjugates were kinetically studied. The effects of the modification degree on the activity and thermostability of dehydrogenases by their hydrophobization were studied and discussed. Practical recommendations for using the dehydrogenase-steroid conjugates in enzyme immunoassay are given.
authors.affiliation: Institute of Bioorganic Chemistry, BSSR Academy of Sciences, Minsk, U.S.S.R.
authors.forename: D I
authors.initials: DI
authors.lastname: Metelitza
authors.forename: A N
authors.initials: AN
authors.lastname: Eryomin
authors.forename: E I
authors.initials: EI
authors.lastname: Karasyova
authors.forename: V L
authors.initials: VL
authors.lastname: Markina
mesh_terms: D002384:Catalysis; D004789:Enzyme Activation; D004795:Enzyme Stability; D005954:Glucosephosphate Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D006358:Hot Temperature; D006854:Hydrocortisone / Q000737:chemistry*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D011374:Progesterone / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D011374:Progesterone; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D006854:Hydrocortisone