An improved method for purifying antihemorrhagic factor in the serum of Habu snake (Trimeresurus flavoviridis).
A new method is presented for purifying antihemorrhagic factor in the serum of Japanese Habu snake (Trimeresurus flavoviridis). The method consists of specific binding of the factor to a hemorrhagic principle-conjugated Sepharose, gel filtration and reverse-phase HPLC. The improved method is simple and rapid, providing the factor with a great increase in specific activity and in a high yield.
Yamakawa
Y
Y
Department of Applied Immunology, National Institute of Health, Tokyo.
Omori-Satoh
T
T
D000818:Animals; D000997:Antivenins / Q000302:isolation & purification*; D001798:Blood Proteins / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006470:Hemorrhage / Q000188:drug therapy; D007525:Isoelectric Focusing; D011485:Protein Binding; D012911:Snakes
D000997:Antivenins; D001798:Blood Proteins; C003941:antihemorrhagic factor