Growth inhibitory effects of transforming growth factor-beta 1 in vivo.
Transforming growth factor-beta (TGF-beta) can reversibly inhibit the in vitro proliferation of murine and human haemopoietic progenitors and some of their more developmentally restricted progeny. Using an assay for measuring day 8 and day 11 CFU-S, TGF-beta caused a gradual decline in the number of CFU-S undergoing DNA synthesis so that after 5 days of daily treatment only quiescent cells were found. Release of this growth inhibition was seen within 24 hours post-treatment with recovery of all progenitors to normal levels. Similar inhibitory effects of TGF-beta were seen on the cells of the intestinal epithelium, indicating that TGF-beta is a general stem cell growth inhibitor. These results suggest that TGF-beta can be used as a cytostatic agent to protect normal stem cells in patients being treated with cell cycle-specific cytotoxic agents.
Migdalska
A
A
Department of Experimental Haematology, Paterson Institute for Cancer Research, Manchester, England.
Molineux
G
G
Demuynck
H
H
Evans
G S
GS
Ruscetti
F
F
Dexter
T M
TM
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003114:Colony-Forming Units Assay; D005260:Female; D006131:Growth Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D007421:Intestine, Small / Q000187:drug effects; D051379:Mice; D013154:Spleen / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000494:pharmacology*
D006131:Growth Inhibitors; D016212:Transforming Growth Factor beta