Electric measurements can be used to monitor the attachment and spreading of cells in tissue culture.
A new electrical assay to measure the attachment and spreading of cells in tissue culture has been developed and substantiated by comparison with a more conventional assay. Small gold electrodes are vacuum deposited on the bottom of standard polystyrene culture dishes and coated with various proteins. As mammalian fibroblasts attach and spread on these surfaces, the measured impedance of the electrodes changes. These impedance changes reflect the amount of area blocked by the spreading cells. Since the weak electrical signals used have no noticeable effects on the cells, this is a very convenient method that is both quantitative and sensitive for measuring cell attachment and spreading.
Mitra
P
P
Rensselaer Polytechnic Institute, Troy, NY 12180-3590.
Keese
C R
CR
Giaever
I
I
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002461:Cell Line, Transformed; D002465:Cell Movement; D003584:Cytological Techniques; D004566:Electrodes; D006046:Gold; D007700:Kinetics; D008722:Methods; D011137:Polystyrenes
D011137:Polystyrenes; D006046:Gold