Long experimental durations are required for double label [14C]- and [3H]2-deoxyglucose autoradiographic methods.
Double-label 2-deoxyglucose (2-DG) studies using sequential [14C]- and [3H]2-DG injections demonstrate increased [14C]2-DG uptake during the first and second stimulation periods. To understand why this occurs, the rat mystacial vibrissae were stimulated at various times following [14C]2-DG injection. Local cerebral glucose utilization (LCGU) increased when whisker stimulation was performed at 0-90 min following [14C]2-DG injection. LCGU did not increase when whisker stimulation was performed at 90-150 min following [14C]2-DG injection. To minimize contamination of the two tracers in double label 2-DG mapping studies, the time between [14C]- and [3H]2-DG administration should be increased to 90 min.
Ciricillo
S F
SF
Department of Neurosurgery, University of California, San Francisco 94121.
Jasper
M P
MP
Gonzalez
M F
MF
Sharp
F R
FR
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001921:Brain / Q000033:anatomy & histology; D001923:Brain Chemistry / Q000502:physiology; D003847:Deoxyglucose; D008297:Male; D010812:Physical Stimulation; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014738:Vibrissae / Q000502:physiology
D003847:Deoxyglucose