LINE-1 repetitive DNA probes for species-specific cloning from Mus spretus and Mus domesticus genomes.
Mus domesticus and Mus spretus mice are closely related subspecies. For genetic investigations involving hybrid mice, we have developed a set of species-specific oligonucleotide probes based on the detection of LINE-1 sequence differences. LINE-1 is a repetitive DNA family whose many members are interspersed among the genes. In this study, library screening experiments were used to fully characterize the species specificity of four M. domesticus LINE-1 probes and three M. spretus LINE-1 probes. It was found that the nucleotide differences detected by the probes define large, species-specific subfamilies. We show that collaborative use of such probes can be employed to selectively detect thousands of species-specific library clones. Consequently, these probes could be exploited to monitor and access almost any given species-specific region of interest within hybrid genomes.
Rikke
B A
BA
Department of Biochemistry, University of Texas Health Science Center, San Antonio 78284.
Hardies
S C
SC
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D015342:DNA Probes; D015723:Gene Library; D016678:Genome; D051379:Mice; D008969:Molecular Sequence Data; D009115:Muridae / Q000235:genetics*; D015345:Oligonucleotide Probes; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D015342:DNA Probes; D015345:Oligonucleotide Probes; D004247:DNA