title: Serial transfer of GVH-R splenomegaly in chicken embryos.
abstract: Using histocompatible chicken strains B14 and B19, we demonstrate that the capacity to induce a GVH-R in an embryo could be induced precociously by the reaction itself. While naive chickens display this capacity around 3 to 4 weeks posthatching, embryos engrafted with adult allogeneic cells at E13 or E8 became endowed with this capacity at E20 and E15, respectively. Furthermore, this acceleration could be obtained by serial transfer of splenic cells through a sequence of three embryos undergoing the GVH-R. The highest efficiency in transfer was realized by regularly alternating the MHC haplotype at each transfer. It is concluded that the original cells from the adult donor may be partially responsible for the transfer, but that cells from the successive embryos are also certainly involved. Thus, maturation of the embryonic immune system appears accelerated by a GVH-R.
authors.affiliation: Institut d'Embryologie Cellulaire et Moléculaire, CNRS, Nogent Sur Marne, France.
authors.forename: J
authors.initials: J
authors.lastname: Desveaux-Chabrol
authors.forename: M
authors.initials: M
authors.lastname: Gendreau
authors.forename: F
authors.initials: F
authors.lastname: Dieterlen-Lièvre
mesh_terms: D000367:Age Factors; D000818:Animals; D002642:Chick Embryo / Q000276:immunology*; D016332:Fetal Tissue Transplantation; D006087:Graft vs Host Reaction; D006239:Haplotypes; D007121:Immunocompetence; D016219:Immunotherapy, Adoptive; D007963:Leukocytes, Mononuclear / Q000637:transplantation*; D008285:Major Histocompatibility Complex; D013154:Spleen / Q000166:cytology / Q000254:growth & development / Q000637:transplantation*; D013163:Splenomegaly / Q000196:embryology / Q000276:immunology*