Polymerase chain reaction for detection of the cholera enterotoxin operon of Vibrio cholerae.
We report a set of oligonucleotide primers and amplification conditions for the polymerase chain reaction to detect the ctx operon of Vibrio cholerae. The results of this assay on strains of V. cholerae and related organisms were identical with those obtained by the DNA colony hybridization test with the polynucleotide probe. The detection limit of this system was 1 pg of chromosomal DNA or broth culture containing three viable cells. The polymerase chain reaction method could directly detect the ctx operon sequences in rice-water stool samples from patients with cholera.
Shirai
H
H
Department of Microbiology, Faculty of Medicine, Kyoto University, Japan.
Nishibuchi
M
M
Ramamurthy
T
T
Bhattacharya
S K
SK
Pal
S C
SC
Takeda
Y
Y
D001483:Base Sequence; D002771:Cholera / Q000175:diagnosis / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D009876:Operon; D016133:Polymerase Chain Reaction / Q000379:methods; D014734:Vibrio cholerae / Q000235:genetics* / Q000302:isolation & purification
D004269:DNA, Bacterial; D004768:Enterotoxins; D015345:Oligonucleotide Probes; C037420:stN protein, Vibrio cholerae