Activity of octreotide acetate in a total nutrient admixture.
The activity of octreotide acetate in a total nutrient admixture (TNA) and the effect of the drug on the stability of lipid emulsion in the TNA were studied. Octreotide acetate injection was added to a standard solution containing 3% lipids, amino acids, dextrose, electrolytes, vitamins, and trace elements to achieve a theoretical concentration of 45 micrograms/dL. Samples were stored at room temperature for 48 hours. Octreotide concentrations were determined in triplicate by radioimmunoassay; physical stability of the solutions was assessed by lipid particle-size determination, pH measurement, and visual observation of emulsion integrity at 0, 12, 24, and 48 hours. The activity of octreotide in two samples of each solution (with and without lipid) was analyzed immediately after preparation and after seven days under refrigeration. There was no evidence of emulsion breakdown or pH change in any solution over the study period. In addition, particle-size distributions at 48 hours and 7 days were comparable to those at time zero, suggesting physical stability. Octreotide acetate activity was not consistently greater than 90% (mean +/- S.D.) after storage for 48 hours. Octreotide acetate at a theoretical concentration of 45 micrograms/dL in a TNA solution containing 3% lipids appeared to be physically compatible for 48 hours at room temperature and for 7 days under refrigeration. However, the chemical activity of octreotide in TNA was not consistent after storage for 48 hours.
Ritchie
D J
DJ
St. Louis College of Pharmacy, Jewish Hospital of St. Louis, MO 63110.
Holstad
S G
SG
Westrich
T J
TJ
Hirsch
J D
JD
O'Dorisio
T M
TM
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004349:Drug Packaging; D004355:Drug Stability; D005217:Fat Emulsions, Intravenous / Q000737:chemistry; D005898:Glass; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000737:chemistry; D015282:Octreotide / Q000008:administration & dosage / Q000494:pharmacology*; D010289:Parenteral Nutrition, Total; D010316:Particle Size; D010969:Plastics; D011863:Radioimmunoassay; D013696:Temperature
D005217:Fat Emulsions, Intravenous; D008055:Lipids; D010969:Plastics; D015282:Octreotide