title: Investigations with enzyme-linked immunosorbent assays for Trichinella spiralis and Toxoplasma gondii in the Dutch 'Integrated Quality Control for finishing pigs' research project.
abstract: In the framework of the Dutch field trial 'Integrated Quality Control (IQC) for finishing pigs' ELISA (screening) techniques were used to detect animals seropositive for Toxoplasma gondii and/or Trichinella spiralis. The aim was to determine whether farms which consistently delivered seropositive pigs could be detected and monitored (defined as 'problem farms'). The investigation involved 120 farms and three slaughterhouses, and a total of 23,348 serum samples were examined. In addition, all pigs were also screened for the presence of Trichinella spiralis with the digestion method (pooled samples). The prevalence of seropositivity for Trichinella spiralis and Toxoplasma gondii was 0.3% and 2.1% respectively. Parasitological examinations concerning T. spiralis were negative. Considering the characteristics of the used methodology, the conclusion was drawn that there were no parasitological or serological indications for T. spiralis infections, and that with respect to T. gondii the infection rate seemed to be equally low for all farms involved. In addition, a longitudinal pilot study during a whole finishing period was undertaken at two finishing farms. Animals seropositive for Toxoplasma gondii were found from the earliest days of the finishing period. Housing and management may (still) play an important role in the prevention of contact with this parasite.
authors.affiliation: Department of the Science of Food of Animal Origin, Faculty of Veterinary Medicine, Utrecht, The Netherlands.
authors.forename: B R
authors.initials: BR
authors.lastname: Berends
authors.forename: J F
authors.initials: JF
authors.lastname: Smeets
authors.forename: A H
authors.initials: AH
authors.lastname: Harbers
authors.forename: F
authors.initials: F
authors.lastname: van Knapen
authors.forename: J M
authors.initials: JM
authors.lastname: Snijders
mesh_terms: D000003:Abattoirs; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000913:Antibodies, Protozoan / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D008137:Longitudinal Studies; D009426:Netherlands / Q000453:epidemiology; D015995:Prevalence; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000453:epidemiology*; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis / Q000453:epidemiology*; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*
chemical_list: D000909:Antibodies, Helminth; D000913:Antibodies, Protozoan