Application of a metal capillary column in gas chromatographic determination of catechol-o-methyltransferase activity.
The utility of a deactivated metal capillary column, Rascot, in the measurement of an enzymatic reaction, in this case measurement of rat catechol-O-methyltransferase activity, was examined. 3,4-Dihydroxybenzaldehyde, 3,4-dihydroxybenzylalcohol and 3,4-dihydroxybenzoic acid were used as substrates and the m- and p-O-methylated products were separated by using Rascot after derivatization. The peaks on the chromatograms were symmetrical. The data obtained were compared with those reported in previously published papers. Good agreement with previous results proved that Rascot is able to withstand practical use in biological materials.
Koh
S
S
Gifu Pharmaceutical University, Mitahora, Japan.
Urayama
K
K
Kawai
S
S
Takayama
Y
Y
D000818:Animals; D002197:Capillary Action; D002394:Catechol O-Methyltransferase / Q000032:analysis* / Q000378:metabolism; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D005260:Female; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D008745:Methylation; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
D002394:Catechol O-Methyltransferase