title: Helicobacter pylori urease: properties and role in pathogenesis.
abstract: Urease (urea amidohydrolase, EC 3.5.1.5) catalyzes the hydrolysis of urea to yield ammonia and carbon dioxide. Research on this enzyme has gained momentum since the discovery of Helicobacter pylori as a causative agent of human gastritis. The remarkably high urease activity of each organism has served as the basis of diagnostic tests for the presence of the organism in the urease biopsy test and urea breath test. Urease undoubtedly plays a central role in H. pylori pathogenesis. Hydrolysis of urea with generation of ammonia may enable survival of this acid-sensitive organism in the gastric mucosa. Ammonia generated by urea hydrolysis may also produce severe cytotoxic effects within gastric epithelium. The enzyme also elicits a strong immune response during acute infection, suggesting that this abundant antigen is readily available to the immune system. An increase in serum IgG titer is predictive of ongoing infection. Much progress has been made with regard to the molecular biology of urease. The high molecular weight protein (estimated by several investigators to be 300-520 kDa) has been purified, revealing two distinct subunits of 29.5 kDa and 66 kDa, a unique subunit structure as compared with other microbial ureases. However, amino acid sequences are nevertheless well conserved when compared with other bacterial ureases and that of the jack bean, Canavalia ensiformis. Furthermore, genes encoding urease of H. pylori have been cloned, sequenced, and amplified by the polymerase chain reaction.
authors.affiliation: Dept. of Medicine, University of Maryland School of Medicine, Baltimore 21201.
authors.forename: H L
authors.initials: HL
authors.lastname: Mobley
authors.forename: L T
authors.initials: LT
authors.lastname: Hu
authors.forename: P A
authors.initials: PA
authors.lastname: Foxal
mesh_terms: D000818:Animals; D004796:Clinical Enzyme Tests; D005753:Gastric Mucosa / Q000382:microbiology; D016481:Helicobacter Infections / Q000175:diagnosis; D016480:Helicobacter pylori / Q000201:enzymology*; D006801:Humans; D014510:Urease / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology
chemical_list: D014510:Urease