title: Characterization of a set of variable number of tandem repeat markers conserved in bovidae.
abstract: Screening purpose-built libraries with minisatellite probes, we have isolated 36 bovine variable number of tandem repeat markers (VNTRs) characterized by a mean heterozygosity of 59.3 within the American Holstein breed. Matching probabilities and exclusion powers were estimated by Monte-Carlo simulation, showing that the top 5 to 10 markers could be used as a very efficient DNA-based system for individual identification and paternity diagnosis. The isolated VNTR systems should contribute significantly to the establishment of a bovine primary DNA marker map. Linkage analysis, use of somatic cell hybrids, and in situ hybridization demonstrate that these bovine VNTRs are scattered throughout the bovine genome, without evidence for proterminal confinement as in the human, and that at least some of them are organized as clusters. Moreover, Southern blot analysis and in situ hybridization demonstrate conservation of sequence and map location of minisatellites within Bovidae.
authors.affiliation: Genmark Inc., Salt Lake City, Utah 84108.
authors.forename: M
authors.initials: M
authors.lastname: Georges
authors.forename: A
authors.initials: A
authors.lastname: Gunawardana
authors.forename: D W
authors.initials: DW
authors.lastname: Threadgill
authors.forename: M
authors.initials: M
authors.lastname: Lathrop
authors.forename: I
authors.initials: I
authors.lastname: Olsaker
authors.forename: A
authors.initials: A
authors.lastname: Mishra
authors.forename: L L
authors.initials: LL
authors.lastname: Sargeant
authors.forename: A
authors.initials: A
authors.lastname: Schoeberlein
authors.forename: M R
authors.initials: MR
authors.lastname: Steele
authors.forename: C
authors.initials: C
authors.lastname: Terry
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002417:Cattle / Q000235:genetics*; D002871:Chromosome Banding; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004276:DNA, Satellite; D008040:Genetic Linkage; D005819:Genetic Markers; D009010:Monte Carlo Method; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004276:DNA, Satellite; D005819:Genetic Markers