Pathophysiological effects of a limulus amoebocyte-lysate-positive substance during haemodialysis.
The washing solution of a dialyser was collected after recirculation of pyrogen-free water through the blood compartment, and the amount of limulus amoebocyte lysate-(LAL)-positive material was measured. Our study shows that the amount of LAL-positive substance on the cuprophane membrane of DIP-02-02 dialyser (USSR) is 5 times greater than in the case of cuprophane membrane of D2 hollow-fibre dialyser (Fresenius). There appears to be an acute increase (0.1 ng/ml) followed by a decline to normal values of LAL-positive substance concentration in the blood of patients in the first hour of haemodialysis using a DIP-02-02 dialyser. We conjecture that the cause of monocyte activation during haemodialysis is the LAL-positive material of the dialyser membrane, which in turn may induce the activation of lipid peroxidation and the increase of prostaglandin E production. However, it is not possible to exclude the likelihood that the origin of endotoxin-like LAL-positive substances lies in the dialysate.
Darenkov
A F
AF
1st Moscow Medical Institute, Institute of Urology, USSR.
Yermolenko
V M
VM
Sinyukhin
V N
VN
Stetsyuk
E A
EA
Kochlov
A P
AP
Yarmolinski
J S
JS
D000328:Adult; D002482:Cellulose / Q000031:analogs & derivatives; D004731:Endotoxins / Q000097:blood*; D006801:Humans; D007688:Kidneys, Artificial; D008033:Limulus Test; D015227:Lipid Peroxidation; D008567:Membranes, Artificial; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D011458:Prostaglandins E / Q000097:blood; D006435:Renal Dialysis / Q000009:adverse effects*
D004731:Endotoxins; D008567:Membranes, Artificial; D011458:Prostaglandins E; D002482:Cellulose; C009865:cuprammonium cellulose