title: Carbon-phosphorus bond cleavage activity in cell-free extracts of Enterobacter aerogenes ATCC 15038 and Pseudomonas sp. 4ASW.
abstract: Carbon-phosphorus bond cleavage activity was investigated in cell-free extracts of Enterobacter aerogenes ATCC 15038 (IFO 12010) and Pseudomonas sp. 4ASW, strains known to utilize a range of phosphonates as sole phosphorus source. In vitro phosphonatase activity was detected in extracts of both organisms; however extensive analysis failed to detect any organic product from phosphonates other than phosphonoacetal dehyde. Non-specific liberation of phosphate was observed in Pseudomonas sp. 4ASW, associated with a single fraction of FPLC-purified extract, and is believed to result from the activity of cellular phosphatases.
authors.affiliation: School of Biology and Biochemistry, Queens University, Belfast.
authors.forename: G
authors.initials: G
authors.lastname: McMullan
authors.forename: R
authors.initials: R
authors.lastname: Watkins
authors.forename: D B
authors.initials: DB
authors.lastname: Harper
authors.forename: J P
authors.initials: JP
authors.lastname: Quinn
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004754:Enterobacter / Q000201:enzymology*; D006867:Hydrolases / Q000378:metabolism; D008190:Lyases / Q000378:metabolism; D063065:Organophosphonates / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology*
chemical_list: D063065:Organophosphonates; D010710:Phosphates; D006867:Hydrolases; C023920:phosphonoacetaldehyde hydrolase; D008190:Lyases; C051328:carbon-phosphorus lyase