deoP1 promoter and operator mutants in Escherichia coli: isolation and characterization.
Plasmid DNA containing deoP1, one of the two major promoters of the deo operon, has been mutagenized using hydroxylamine, and promoter down-mutations and operator mutations were selected. The isolated mutants are all located within a 16 bp palindromic sequence containing the -10 region of deoP1. The results show that RNA polymerase and DeoR repressor compete for the same DNA target. The deoP1 promotor activity is dependent on a TG motif one base pair upstream of the -10 consensus sequence. The sequence of the deo operator site was further verified by use of a synthetic linker.
Dandanell
G
G
Institute of Biological Chemistry B, University of Copenhagen, Denmark.
Hammer
K
K
D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000494:pharmacology; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009838:Oligodeoxyribonucleotides; D009876:Operon; D010641:Phenotype; D010957:Plasmids / Q000187:drug effects*; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid
D004269:DNA, Bacterial; D006898:Hydroxylamines; D009838:Oligodeoxyribonucleotides; D019811:Hydroxylamine