The fate of human polymorphonuclear leukocyte aminopeptidases upon cell stimulation with phagocytic and chemical stimuli.
1. After being treated with nonopsonized zymosan A or phorbol-12-myristate-13-acetate, human polymorphonuclear leukocytes release aminopeptidases together with granules marker-enzymes and vitamin B12-binding protein. 2. Chemotactic peptide, fMet-Leu-Phe and its analogs, fMet-Phe, fMet-Ala and fMet-Leu-Phe-Lys have a similar effect. 3. By their isoelectric point determinations the released aminopeptidases correspond to the enzymes from granules. Among aminopeptidases released the highest activities were those toward methionine- and alanine-2-naphthylamide and the lowest one toward arginine-2-naphthylamide.
Grdisa
M
M
Department of Organic Chemistry and Biochemistry, Ruder Bosković Institute, Zagreb, Yugoslavia.
Vitale
L
L
D000626:Aminopeptidases / Q000378:metabolism*; D015550:Cell Degranulation; D002455:Cell Division; D006801:Humans; D007525:Isoelectric Focusing; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000031:analogs & derivatives / Q000494:pharmacology; D009504:Neutrophils / Q000201:enzymology* / Q000502:physiology; D010587:Phagocytosis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D009240:N-Formylmethionine Leucyl-Phenylalanine; C049813:N-formylmethionyl-leucyl-phenylalanyl-lysine; D015054:Zymosan; D000626:Aminopeptidases; D013755:Tetradecanoylphorbol Acetate