title: [Preparation of immunoenzyme conjugates of beta-lactamase from Bacillus licheniformis 749/c and horseradish peroxidase with human antibodies to HIV-1].
abstract: By using three different linkage methods with carbodiimide, glutaraldehyde and periodite, immunoenzyme conjugates of beta-lactamase from Bacillus licheniformis 749/c and horse radish peroxidase with human antibodies to HIV-1 were prepared. The human antibodies were purified by the affinity procedure on Protein-A-Sepharose 6B. The conjugates were tested in a solid phase immunoenzymatic system for the HIV-1 antigen. It was shown that the conjugates prepared by the carbodiimide linkage method had the highest titer, the beta-lactamase conjugate being superior by its titer to the respective peroxidase conjugate. In the lyophilized state the conjugates prepared with the carbodiimide linkage method were stable.
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Sazykin
authors.forename: S A
authors.initials: SA
authors.lastname: Eremin
authors.forename: A M
authors.initials: AM
authors.lastname: Egorov
authors.forename: A I
authors.initials: AI
authors.lastname: Starov
authors.forename: I A
authors.initials: IA
authors.lastname: Sakaian
authors.forename: G R
authors.initials: GR
authors.lastname: Matsevich
authors.forename: R A
authors.initials: RA
authors.lastname: Gibadulin
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D015483:HIV Antibodies / Q000276:immunology*; D006735:Horseradish Peroxidase / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D001618:beta-Lactamases / Q000276:immunology*
chemical_list: D015483:HIV Antibodies; D006735:Horseradish Peroxidase; D001618:beta-Lactamases
vernacular_title: Poluchenie immunofermentnykh kon"iugatov beta-laktamazy iz Bacillus licheniformis 749/c i peroksidazy khrena s chelovecheskimi antitelami k VICh-1.