Effect of glucose on the expression of c-myc gene in cultured RINm5F cell.
The study was designed to examine the effect of glucose on the expression of c-myc gene in cultured RINm5F cells. After monolayer culture was established in RPMI 1640 media supplemented with 10% fetal calf serum (FCS), the cells were cultured in various concentrations of glucose and 1 or 10% FCS for another 24 hours. A mRNA was extracted from the cultured cells by a single step method, and Northern analysis was done to detect RNA band. A 0.5 kilobase single band was detected as c-myc mRNA. The expression of c-myc gene mRNA was reduced with increased concentration of glucose with 1% FCS. However, supplementation of 10% FCS abolished the effect of glucose on expression of c-myc gene. These findings suggested that glucose in conjunction with other growth promoting factors played an important role in expression of oncogene and cell growth in RINm5F cells.
Kim
S W
SW
Department of Internal Medicine, Kyung Hee University School of Medicine, Seoul, Korea.
Yang
I M
IM
Kim
J W
JW
Kim
Y S
YS
Kim
K W
KW
Choi
Y K
YK
Suh
K S
KS
D000818:Animals; D002460:Cell Line; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D016259:Genes, myc / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology*; D007340:Insulinoma / Q000235:genetics*; D010190:Pancreatic Neoplasms / Q000235:genetics*; D051381:Rats; D014407:Tumor Cells, Cultured
D005947:Glucose