Chemiluminescence dot blot hybridization assay for detection of B19 parvovirus DNA in human sera.
A chemiluminescence dot blot hybridization assay was used for the detection of B19 parvovirus DNA in human sera by using digoxigenin-labeled probes. The probes were revealed immunoenzymatically by use of anti-digoxigenin Fab fragments conjugated with alkaline phosphatase. The chemiluminescence signal was obtained by reacting the labeled probe-target complex with an enzyme-triggerable dioxetane substrate. The emitted photons were detected with instant photographic films. In the search for B19 parvovirus DNA, 2,808 serum samples were analyzed.
Musiani
M
M
Istituto di Microbiologia, Universitá di Bologna, Italy.
Zerbini
M
M
Gibellini
D
D
Gentilomi
G
G
Venturoli
S
S
Gallinella
G
G
Ferri
E
E
Girotti
S
S
D015342:DNA Probes; D004279:DNA, Viral / Q000097:blood* / Q000235:genetics; D004076:Digoxigenin; D016731:Erythema Infectiosum / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D015151:Immunoblotting / Q000379:methods* / Q000706:statistics & numerical data; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization; D016732:Parvovirus B19, Human / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D015342:DNA Probes; D004279:DNA, Viral; D004076:Digoxigenin