title: Purification of S-adenosylmethionine decarboxylase from soybean.
abstract: S-Adenosylmethionine decarboxylase (EC 4.1.1.19) was purified to homogeneity from the cytosol of soybean (Glycine max) axes by ammonium sulfate fractionation, DEAE-Sepharose and methylglyoxalbis(guanylhydrazone)-Sepharose 6B chromatographies. The enzyme was free from diamine oxidase activity. The molecular weight of the enzyme estimated by gel filtration and sodium dodecyl sulfate polyacrylamide gel electrophoresis was 66,000. The Km value for S-adenosylmethionine was 0.26 mM. The optimum pH and temperature were 7.5 and 40 degrees C. Neither putrescine nor Mg2+ affected the enzyme activity, but the enzyme was inhibited by spermidine, spermine, methylglyoxalbis(guanylhydrazone), sodium borohydride and phenylhydrazine. Agmatine was a novel inhibitor which inhibited S-adenosylmethionine decarboxylase and arginine decarboxylase, preventing the accumulation of decarboxylated S-adenosylmethionine and putrescine, respectively.
authors.affiliation: Department of Biochemistry, College of Science, Yonsei University, Seoul, Korea.
authors.forename: Y G
authors.initials: YG
authors.lastname: Yang
authors.forename: Y D
authors.initials: YD
authors.lastname: Cho
mesh_terms: D012437:Adenosylmethionine Decarboxylase / Q000302:isolation & purification* / Q000378:metabolism; D000376:Agmatine / Q000494:pharmacology; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006706:Homeostasis; D007700:Kinetics; D008970:Molecular Weight; D011073:Polyamines / Q000494:pharmacology; D013025:Glycine max / Q000201:enzymology*
chemical_list: D011073:Polyamines; D000376:Agmatine; D012437:Adenosylmethionine Decarboxylase