Expression of actin and myosin genes during PC12 cell differentiation.
We have measured the accumulation of transcripts for myosin and actin during NGF induced differentiation of PC12 cells. Beta (beta) and gamma (gamma) actin and myosin light chains (MLC) 2 and 3 show different patterns of expression, with transient elevations in gene expression one day after NGF addition. This elevation occurs earlier than that of neurite outgrowth, neurofilament protein (NF68) (16) and Thy-1 glycoprotein gene expression. These results suggest differing mechanisms of control of actin and myosin expression, together with a varying function and relationship between them during NGF-induced neurite differentiation.
Henke
R C
RC
Department of Muscle Genetics, Children's Medical Research Foundation, Camperdown NSW, Australia.
Tolhurst
O
O
Sentry
J W
JW
Gunning
P
P
Jeffrey
P L
PL
D008841:Actin Cytoskeleton / Q000502:physiology*; D000199:Actins / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D009218:Myosins / Q000096:biosynthesis / Q000235:genetics*; D009414:Nerve Growth Factors / Q000502:physiology; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D016716:PC12 Cells
D000199:Actins; D009414:Nerve Growth Factors; D009218:Myosins