title: A new distinct group of 2 S albumins from rapeseed. Amino acid sequence of two low molecular weight napins.
abstract: Two napins (nIa and nIb), isolated from Brassica napus (rapeseed) seeds, have been sequenced. The two proteins show the common structural pattern of the 2 S albumins, since they are composed of two disulfide-linked chains of different size, yet they exhibit an atypical low molecular weight (12.5 kDa vs. 14.5 kDa of the major napins). High sequence similarity has been found between these 2 proteins, but only 54% similarity can be estimated from their comparison with the 14.5 kDa major napins. Thus, nIa and nIb are considered representatives of a new distinct group of rapeseed napins since all the previously known napins exhibit 95% sequence similarity. Unexpectedly, the similarity increases when compared with the 2 S proteins from other species.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad Complutense, Madrid, Spain.
authors.forename: R I
authors.initials: RI
authors.lastname: Monsalve
authors.forename: C
authors.initials: C
authors.lastname: Lopez-Otin
authors.forename: M
authors.initials: M
authors.lastname: Villalba
authors.forename: R
authors.initials: R
authors.lastname: Rodríguez
mesh_terms: D055516:2S Albumins, Plant; D000418:Albumins / Q000737:chemistry* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D001937:Brassica / Q000737:chemistry* / Q000235:genetics; D010450:Endopeptidases; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D010944:Plants / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D055516:2S Albumins, Plant; D000418:Albumins; D010446:Peptide Fragments; D010940:Plant Proteins; C050363:napin protein, Brassica napus; D010450:Endopeptidases