Crystal-neutrophil interactions lead to interleukin-1 synthesis.
Normal human blood neutrophils were studied for their capacity to synthesize and release interleukin-1 (IL-1) species after phagocytosis of triclinic monosodium urate (MSU) and calcium pyrophosphate dihydrate crystals (CPPD). MSU crystals were more potent inducers of IL-1 generation than CPPD or unopsonized zymosan. Microcrystal-stimulated neutrophils characteristically secreted most of the newly synthesized IL-1. Colchicine partly inhibited the secretion of IL-1 by neutrophils during phagocytosis of solid particles. However, colchicine selectively inhibited IL-1 synthesis induced by microcrystals. These results suggest that neutrophil-derived IL-1 may contribute to the pathogenesis of crystal-induced arthritis.
Roberge
C J
CJ
Unité de Recherche Inflammation et Immunologie-Rhumatologie, Centre de Recherche du CHUL, Boul Laurier, Québec, Canada.
Grassi
J
J
De Médicis
R
R
Frobert
Y
Y
Lussier
A
A
Naccache
P H
PH
Poubelle
P E
PE
D002131:Calcium Pyrophosphate; D003078:Colchicine / Q000494:pharmacology; D003460:Crystallization; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis*; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010587:Phagocytosis / Q000502:physiology*; D014527:Uric Acid; D015054:Zymosan / Q000494:pharmacology
D007375:Interleukin-1; D014527:Uric Acid; D015054:Zymosan; D003078:Colchicine; D002131:Calcium Pyrophosphate