title: Experimental traction retinal detachment in the cat.
abstract: We developed a reproducible model of traction retinal detachment (TRD) in the cat eye by creating a serous retinal detachment and then injecting 2.5 x 10(5) kitten dermal fibroblasts into the vitreous cavity at the site of a retinal wound. Serous detachments were produced by exposing an area of retina to focused light after intravenous injection of rose bengal (a photosensitizing dye). TRD developed rapidly within the first 2 weeks after fibroblast injection, accompanied by the formation of vitreoretinal strands and, to a lesser degree, epiretinal and/or subretinal proliferation. Histopathology demonstrated fibroblasts within the vitreous or along the posterior hyaloid face. Focal deposits of fibroblasts were occasionally found on the inner surface of the retina and/or in the subretinal space. Fibroblast proliferation was confirmed by uptake of radiolabeled thymidine. Deposition of collagen was noted at as early as 3 days after fibroblast injection. Neovascularization was not observed. Control eyes that did not receive fibroblasts showed resolution of serous detachment without retinal traction. In all eyes, retinal degeneration and thinning were seen in the area of previous photodynamic treatment. In this model of TRD, anteroposterior traction (due to vitreous strands) predominates, as is observed in experimental posterior penetrating ocular injury induced by intravitreal blood injection, which also results in vitreous strand formation. Our model, however, enables clinical assessment of TRD in the cat without the media opacification produced by vitreous blood.
authors.affiliation: Durham Veterans Administration Medical Center, NC.
authors.forename: C A
authors.initials: CA
authors.lastname: Wilson
authors.forename: J A
authors.initials: JA
authors.lastname: Khawly
authors.forename: D L
authors.initials: DL
authors.lastname: Hatchell
authors.forename: R
authors.initials: R
authors.lastname: Machemer
mesh_terms: D000818:Animals; D002415:Cats; D004195:Disease Models, Animal; D005260:Female; D005347:Fibroblasts; D008027:Light; D008297:Male; D015203:Reproducibility of Results; D012163:Retinal Detachment / Q000209:etiology / Q000473:pathology*; D012395:Rose Bengal
chemical_list: D012395:Rose Bengal