title: ras p21 expression in relation to DNA ploidy, S-phase fraction and prognosis in colorectal adenocarcinoma.
abstract: ras p21 expression, as indicated by the monoclonal antibody ras 11, was estimated using immunohistochemistry on 69 primary colorectal adenocarcinomas. Also, DNA ploidy and S-phase fraction (SPF) were analysed with flow cytometry. Positive staining for ras 11 tended to be more common in DNA non-diploid tumours (P = 0.11), but was significantly correlated with high SPF (P = 0.038). Positive ras 11 staining, Dukes' stage, DNA ploidy and SPF were related to the recurrence-free interval of patients with Dukes' A-C tumours (P = 0.0014, P = 0.023, P = 0.035 and P = 0.040, respectively). ras 11 staining was a prognostic factor independent of both Dukes' stage and DNA ploidy (P = 0.011). The results indicate that pan ras p21 expression is associated with proliferative activity and has an independent prognostic value in colorectal adenocarcinoma.
authors.affiliation: Department of Oncology, University Hospital, Linköping, Sweden.
authors.forename: X F
authors.initials: XF
authors.lastname: Sun
authors.forename: S
authors.initials: S
authors.lastname: Wingren
authors.forename: J M
authors.initials: JM
authors.lastname: Carstensen
authors.forename: O
authors.initials: O
authors.lastname: Stål
authors.forename: T
authors.initials: T
authors.lastname: Hatschek
authors.forename: B
authors.initials: B
authors.lastname: Boeryd
authors.forename: B
authors.initials: B
authors.lastname: Nordenskjöld
authors.forename: H
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D011905:Genes, ras; D006801:Humans; D009367:Neoplasm Staging; D016281:Oncogene Protein gp140(v-fms) / Q000096:biosynthesis*; D011003:Ploidies; D011379:Prognosis; D016196:S Phase
chemical_list: D004273:DNA, Neoplasm; D016281:Oncogene Protein gp140(v-fms)