Cloning and expression of penicillin G acylase gene in Bacillus megaterium.
Bacillus megaterium BM1, which produces penicillin G acylase (PGA), has been isolated. Gene encoding for PGA was cloned into E. coli MC1061 using pBR322 as the vector, obtaining a recombinant plasmid pBmPA4 containing 9.9 kb inserted DNA. Restriction map of the plasmid was analyzed. A pBmPA5 containing 4.9 kb was gained by deletion in vitro. Both pBmPA4 and pBmPA5 clones can be expressed in E.coli MC1061, and their expressions were induced by phenylacetic acid.
Zhang
L F
LF
Institute of Biophysics, Chinese Academy of Sciences, Beijing.
Li
Z W
ZW
Zhang
Q J
QJ
D001410:Bacillus megaterium / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D010399:Penicillin Amidase / Q000235:genetics*; D010957:Plasmids; D015183:Restriction Mapping
D010399:Penicillin Amidase