title: Time-course variations of effective proton conductance and GDP binding in brown adipose tissue mitochondria of rats during prolonged cold exposure.
abstract: 1. Time-course variations of the thermogenic pathway in rat brown adipose tissue (BAT) mitochondria were examined. 2. Several parameters of mitochondrial energization, protonmotive force and its components pH gradient and membrane potential were investigated. The specific binding of GDP was compared with the effective proton conductance (CmH+) of the membrane. 3. Ten-days cold exposure led to maximal GDP binding and GDP-dependent CmH+. 4. The subsequent relative decrease in GDP binding observed during prolonged cold exposure (40 days) was functional and led to a lower GDP-dependent CmH+. CmH+ showed greater variation than GDP binding. 5. The CmH+ decrease was not due to a masking of active sites of the uncoupling protein. 6. Basal GDP-independent CmH+ was not modified. 7. Results are discussed with reference to the significance of biochemical measures and to the physiological regulation of BAT thermogenesis.
authors.affiliation: Laboratoire d'Adaptation Energétique à l'Environnement, E.P.H.E., Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Goubern
authors.forename: M F
authors.initials: MF
authors.lastname: Chapey
authors.forename: R
authors.initials: R
authors.lastname: Portet
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D000818:Animals; D003080:Cold Temperature; D006153:Guanosine Diphosphate / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D008564:Membrane Potentials; D008928:Mitochondria / Q000378:metabolism* / Q000502:physiology; D051381:Rats
chemical_list: D006153:Guanosine Diphosphate