Release of a small two-chain form of antithrombin III from a conformationally changed antithrombin III-thrombin complex.
Reaction of antithrombin III (AT) with thrombin results in the formation of stable antithrombin III-thrombin (AT-T) complex with a Mr of 92.5-kDa, accompanied by the appearance of a proteolytically modified form of the inhibitor (ATM). Under these conditions AT-T is also transformed to a smaller complex (AT-TS). This smaller complex (81-kDa), a product of a conformational change at the AT moiety of the AT-T complex, is further transformed to a very small complex (AT-TVS) with a Mr of 71-kDa. Along with this process, AT-TS slowly dissociates to a free enzyme and a small, presumably two-chain product of AT (ATMS) with a Mr of 49-kDa. The newly described component, ATMS, naturally occurs in plasma and serum and accumulates significantly in plasma of patients suffering from cardiovascular disease.
Knoller
S
S
Maurice and Gabriela Goldschleger Eye Research Institute, Sackler Faculty of Medicine, Tel-Aviv University Chaim Sheba Medical Center, Tel-Hashomer, Israel.
Savion
N
N
D000595:Amino Acid Sequence; D000990:Antithrombin III / Q000378:metabolism*; D001797:Blood Protein Electrophoresis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000494:pharmacology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*
D006898:Hydroxylamines; D010446:Peptide Fragments; C046193:antithrombin III-protease complex; D019811:Hydroxylamine; D000990:Antithrombin III; D010447:Peptide Hydrolases