Distribution of acetylated alpha-tubulin in brain. In situ localization and biochemical characterization.
We studied the solubility properties of brain acetylated alpha-tubulin, as well as the localization of this tubulin in brain tissue. Endogenous unpolymerized tubulin and cytoskeletal tubulin were fractionated after brain Triton-solubilization. Using the immunoblotting technique, we found that acetylated alpha-tubulin was recovered in the cytoskeletal fraction, and that most (92%) of the acetylated microtubules of this fraction were depolymerized by cold/Ca2+ treatment. In another set of experiments, axonal and soma-dendritic preparations were found to have equivalent amounts of acetylated alpha-tubulin. By immunogold electron microscopy, we established that acetylated microtubules are widely distributed in dendrites of the central nervous system.
Morales
M
M
Department of Psychology, University of Colorado, Boulder 80309.
Fifková
E
E
D000107:Acetylation; D000818:Animals; D001369:Axons / Q000648:ultrastructure; D001921:Brain / Q000648:ultrastructure*; D001923:Brain Chemistry; D002458:Cell Fractionation; D003599:Cytoskeleton / Q000648:ultrastructure; D003712:Dendrites / Q000648:ultrastructure; D006624:Hippocampus / Q000648:ultrastructure; D008297:Male; D016253:Microscopy, Immunoelectron; D008870:Microtubules / Q000648:ultrastructure*; D011712:Pyramidal Tracts / Q000648:ultrastructure; D051381:Rats; D011916:Rats, Inbred F344; D014404:Tubulin / Q000032:analysis*
D014404:Tubulin