[The determination of the species specificity of Leishmania antibodies in different groups of human sera].
Species-specificity of two serological tests has been compared: Indirect immunofluorescence test and C-ELISA using antigens prepared of 7 Leishmania species of various groups of human sera, including sera from healthy donors; sera of patients with various tropical parasitoses; sera containing rheumatoid factor and antinuclear antibodies; sera of patients with visceral leishmaniasis (VL); sera of normal subjects arrived from regions endemic for VL; sera of students arrived in Czechoslovakia from various tropical countries endemic for leishmaniasis. It has been shown that C-ELISA (and EIA with corpuscular antigen), being highly sensitive techniques, are very promising for the identification of species-specific Leishmania antibodies. C-ELISA titres beginning from 1:800 and above may be considered as species-specific. With the titre 1:400, cross-reactions with antigens of various Leishmania species are observed.
Giboda
M
M
Goncharov
D B
DB
Saf'ianova
V M
VM
Gracheva
L I
LI
Ditrich
O
O
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D014943:Global Health; D006801:Humans; D007891:Leishmania / Q000276:immunology*; D007893:Leishmania donovani / Q000276:immunology; D007898:Leishmaniasis, Visceral / Q000276:immunology; D010272:Parasitic Diseases / Q000276:immunology; D013045:Species Specificity
D000913:Antibodies, Protozoan
Opredelenie vidospetsificheskikh leĭshmaniĭnykh antitel v raznykh gruppakh syvorotok ot liudeĭ.