Interaction of a synthetic peptide of the interferon alpha-2 C-terminal part with human blood leukocytes. Binding to peripheral blood mononuclear cells.
A biologically active synthetic peptide, 2438, representing the 124-138 amino acid sequence of the human interferon alpha-2 (IFN alpha-2) molecule, which is known to possess IFN-like antiproliferative activity, specifically binds to human blood leukocytes. Scatchard plots reveal two different Kd values, for the 'low' and 'high' affinity binding. The interaction of the 125I-labelled peptide 2438 with the cells is not impaired by human IFN alpha-2 or cholera toxin.
Danilkovitch
A V
AV
Moscow State University, Biological Faculty, USSR.
Kharitonenkov
A I
AI
Freze
K V
KV
Shevalier
A F
AF
Kolosova
O V
OV
Bulargina
T V
TV
Kirkin
A F
AF
Gusev
M V
MV
D001402:B-Lymphocytes / Q000378:metabolism*; D001665:Binding Sites; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D016898:Interferon-alpha / Q000378:metabolism*; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
D016898:Interferon-alpha; D010455:Peptides