title: Automated direct high-performance liquid chromatographic assay for estetrol, estriol, cortisone and cortisol in serum and amniotic fluid.
abstract: An automated direct assay for the simultaneous determination of unconjugated estetrol, estriol, cortisone and cortisol in serum and amniotic fluid, using high-performance liquid chromatography with electrochemical detection and ultraviolet detection, has been developed. The analysis time is ca. 1 h. This system offers good reproducibility with low coefficients of variation (estetrol, 2.3%; estriol, 2.3%; cortisone, 2.6%; cortisol, 1.9%). Detection limits are low enough for routine determinations (estetrol and estriol, 150 pg; cortisone and cortisol, 5 ng). Comparison of the values measured by the present method and by radioimmunoassay revealed significant correlations for estetrol (r = 0.787, p less than 0.01), estriol (r = 0.957, p less than 0.01), cortisone (r = 0.956, p less than 0.01) and cortisol (r = 0.865, p less than 0.01). This system proved to be valuable in monitoring feto-placental function.
authors.affiliation: Department of Obstetrics and Gynecology, Okayama University Medical School, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Noma
authors.forename: N
authors.initials: N
authors.lastname: Hayashi
authors.forename: K
authors.initials: K
authors.lastname: Sekiba
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003348:Cortisone / Q000032:analysis* / Q000097:blood*; D004953:Estetrol / Q000032:analysis* / Q000097:blood*; D004964:Estriol / Q000032:analysis* / Q000097:blood*; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000032:analysis / Q000097:blood*; D011247:Pregnancy
chemical_list: D004953:Estetrol; D004964:Estriol; D003348:Cortisone; D006854:Hydrocortisone