[Study on the elimination of bacteria from the cultures of Lyme disease spirochetes (Borrelia burgdorferi)].
Contaminated cultures of strains H11, H20 from ticks, and strain R9 from patient were purified by antisera for bacteria, chemicals, filtration dilution and animal inoculation. Most of bacteria were eliminated after adding antisera into contaminated strains H11, H20. The preliminarily decontaminated cultures of strains H11, H20 or those with a few bacteria such as strain R9 could be completely purified by filtration, chemicals, dilution, or animal inoculation, but filtration is the simplest and the most effective purification method.
Wan
K
K
Institute of Epidemiology and Microbiology, Chinese Academy of Prevetive Medicine.
D000818:Animals; D015748:Borrelia burgdorferi Group / Q000254:growth & development* / Q000302:isolation & purification*; D006224:Cricetinae; D003470:Culture Media; D005260:Female; D005374:Filtration; D006801:Humans; D008193:Lyme Disease / Q000382:microbiology; D008297:Male; D008581:Meningitis / Q000382:microbiology; D013987:Ticks / Q000382:microbiology
D003470:Culture Media