title: Insertional versus targeted mutagenesis in mice.
abstract: Recent innovations in mutagenesis techniques for mice have the potential to revolutionize the molecular genetic analysis of mouse development. Insertional mutagenesis by the introduction of exogenous DNA into the mouse germline hs permitted the molecular cloning and analysis of several novel genes important for early embryonic development. Targeted mutagenesis by homologous recombination in embryonic stem cells permits, in theory, the production of mutations in any cloned gene. The complementary information being obtained from these two mutagenesis procedures is shedding new light on the genes important for early mouse development, and the roles these genes play in that process.
authors.affiliation: Department of Cell and Developmental Biology, Roche Institute of Molecular Biology, Nutley, NJ 07110.
authors.forename: T
authors.initials: T
authors.lastname: Gridley
mesh_terms: D000483:Alleles; D000818:Animals; D004195:Disease Models, Animal; D005796:Genes; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000172:deficiency / Q000235:genetics; D051379:Mice / Q000235:genetics*; D016254:Mutagenesis, Insertional; D016297:Mutagenesis, Site-Directed
chemical_list: D007041:Hypoxanthine Phosphoribosyltransferase