Effect of benzoic acid on glycolytic metabolite levels and intracellular pH in Saccharomyces cerevisiae.
Low concentrations of benzoic acid stimulated fermentation rates in Saccharomyces cerevisiae. At concentrations near the maximum permitting growth, there was inhibition of fermentation, lowered ATP and intracellular pH, and relatively greater accumulation of benzoate. Changes in the levels of glycolytic intermediates suggested that fermentation was inhibited as a result of high ATP usage rather than of lowered intracellular pH. Specific inhibition of phosphofructokinase or of several other glycolytic enzymes was not observed.
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Division of Food Processing, Commonwealth Scientific and Industrial Research Organization, North Ryde, New South Wales, Australia.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001565:Benzoates / Q000378:metabolism / Q000494:pharmacology*; D019817:Benzoic Acid; D005285:Fermentation / Q000187:drug effects; D006019:Glycolysis / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
D001565:Benzoates; D000255:Adenosine Triphosphate; D019817:Benzoic Acid