Assessment of in vivo mutagenic potency of ethylenediaminetetraacetic acid in albino mice.
Ethylenediaminetetraacetic acid (EDTA) disodium salt, a widely used metal chelator, was studied for its potency to induce bone marrow micronuclei, dominant lethal mutations and sperm-head abnormalities in albino mice. The acute oral LD50 dose computed by probit regression was 30 mg/kg body weight in the strain used. Preliminary studies showed that oral administration of EDTA disodium salt at doses of 5, 10 and 15 mg/kg body weight/day on 5 consecutive days did not induce any obvious signs of toxicity. In the bone marrow micronucleus assay acute doses of EDTA disodium salt (5-20 mg/kg body weight) induced a dose-dependent increase in the incidence of micronucleated polychromatic erythrocytes at a 24-hr sampling. However, administration at doses of 5, 10 and 15 mg/kg for 5 consecutive days did not produce any observable effect on either the testicular or epididymal weights and histology. No appreciable alterations were observed in the caudal sperm counts at any of the sampling intervals and there was no treatment-related increase in the incidence of sperm-head abnormalities. Furthermore, treatment of male mice with EDTA disodium salt (10 mg/kg body weight/day for 5 consecutive days) induced no increase in the incidence of post-implantation embryonic deaths, except for a marginal but statistically insignificant increase during wk 2 and 3 of mating.
Muralidhara
Department of Nutrition and Food Safety, Central Food Technological Research Institute, Mysore, India.
Narasimhamurthy
K
K
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004492:Edetic Acid / Q000633:toxicity*; D005260:Female; D005799:Genes, Dominant; D005804:Genes, Lethal; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D011247:Pregnancy; D013076:Sperm Count / Q000187:drug effects; D013077:Sperm Head / Q000187:drug effects
D009153:Mutagens; D004492:Edetic Acid