title: Screening for antibodies associated with halothane hepatitis.
abstract: The diagnosis of halothane hepatitis (HH) may be assisted by detection of antibodies reacting to trifluoroacetylated proteins (anti-TFA antibodies). An enzyme-linked immunosorbent assay (ELISA) utilizing trifluoroacetylated rabbit serum albumin (TFA-RSA) as antigen detected anti-TFA antibodies in 67% of sera from patients for whom a clinical diagnosis of HH was made. Anti-TFA antibodies were detected in 33% of sera when using an ELISA with liver microsomal protein from halothane-treated rabbits as antigen. Absorption of the sera with untreated rabbit liver microsomal protein before using the microsomal protein ELISA resulted in detection of anti-TFA antibodies in 42% of sera. Using the presumptive hapten N-epsilon-trifluoroacetyl-1-lysine to block antibody binding in an ELISA resulted in positive detection in 50% of sera: the results did not always agree with the other ELISA methods. The TFA-RSA ELISA was the most sensitive method and, combined with the TFA-lysine blocking ELISA, resulted in 92% of sera from HH patients testing positive for HH-associated antibodies.
authors.affiliation: Department of Anesthesiology, University of Arizona, College of Medicine, Tucson 85724.
authors.forename: K L
authors.initials: KL
authors.lastname: Hastings
authors.forename: C
authors.initials: C
authors.lastname: Thomas
authors.forename: A K
authors.initials: AK
authors.lastname: Hubbard
authors.forename: A J
authors.initials: AJ
authors.lastname: Gandolfi
mesh_terms: D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000175:diagnosis* / Q000209:etiology / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005463:Fluoroacetates; D006221:Halothane / Q000009:adverse effects / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000032:analysis*; D006801:Humans; D007223:Infant; D008239:Lysine / Q000031:analogs & derivatives / Q000276:immunology; D008297:Male; D008862:Microsomes, Liver / Q000276:immunology; D011817:Rabbits; D012709:Serum Albumin / Q000276:immunology; D014269:Trifluoroacetic Acid / Q000276:immunology
chemical_list: D005463:Fluoroacetates; D006508:Hepatitis Antibodies; C072409:N-epsilon-trifluoro-lysine; D012709:Serum Albumin; C053344:trifluoroacetyl-serum albumin; D014269:Trifluoroacetic Acid; D008239:Lysine; D006221:Halothane