title: Application of bromodeoxyuridine immunohistochemistry to the rat temporomandibular joint.
abstract: To detect S-phase cells in the decalcified paraffin-embedded temporomandibular joint (TMJ) tissues of the rat, bromodeoxyuridine (BrdUrd) immunohistochemistry was performed. Percardiac perfusion of 10% EDTA-2Na Tris buffer accelerated the decalcification. The use of the monoclonal antibody to BrdUrd enabled the visualization of the distribution of S-phase cells. This method is practical in studying cell kinetics and the specific proliferation mode of the TMJ.
authors.affiliation: Second Department of Oral and Maxillofacial Surgery, Nagasaki University, School of Dentistry, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Sekine
authors.forename: K
authors.initials: K
authors.lastname: Sano
authors.forename: R
authors.initials: R
authors.lastname: Hironaka
authors.forename: T
authors.initials: T
authors.lastname: Inokuchi
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D001973:Bromodeoxyuridine; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D016196:S Phase; D013704:Temporomandibular Joint / Q000737:chemistry / Q000166:cytology*
chemical_list: D001973:Bromodeoxyuridine