Determination of non-immune binding of immunoglobulin G to Staphylococcus aureus by enzyme-linked immunosorbent assays.
Non-immune binding of human IgG to Staphylococcus aureus was studied by enzyme-linked immunosorbent assays using whole bacteria or bacterial cell walls as the solid phase. Two types of anti-human IgG peroxidase conjugates each with a low affinity for protein A, were used: F(ab')2-fragments of goat IgG and chicken IgY.
Linder
L E
LE
Department of Oral Microbiology, Karolinska Institute, Stockholm, Sweden.
Shockman
G D
GD
Sund
M L
ML
D002473:Cell Wall / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D010544:Peroxidases / Q000737:chemistry; D011485:Protein Binding; D013205:Staphylococcal Protein A / Q000378:metabolism; D013211:Staphylococcus aureus / Q000378:metabolism*
D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D010544:Peroxidases