title: [A rapid method of preparing the (H3-H4-H2A-H2b)(2) histone octamer in large quantities].
abstract: A simple and fast method for isolation of large amounts of the histone octamer (H2A-H2B-H3-H4)2 is proposed. This method is based on chromatin adsorption by hydroxyapatite with subsequent extraction of the histone octamer with 50 mM sodium-phosphate buffer containing 4 M NaCl pH 8.0. It was shown that the properties of the histone octamer isolated by this extractive procedure are identical with those of the histone octamer obtained by elution on a Sephadex G-100 column. The histone tetramer (H3-H4)2 and dimer (H2A-H2B) were obtained after gel filtration on Sephadex G-100 in 50 mM sodium-acetate (pH 5.6).
authors.forename: V I
authors.initials: VI
authors.lastname: Tiulenev
authors.forename: L A
authors.initials: LA
authors.lastname: Konoplich
authors.forename: A A
authors.initials: AA
authors.lastname: Krivonos
authors.forename: S N
authors.initials: SN
authors.lastname: Khrapunov
mesh_terms: D000818:Animals; D002417:Cattle; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000737:chemistry; D002843:Chromatin / Q000737:chemistry; D002850:Chromatography, Gel; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000737:chemistry / Q000302:isolation & purification*; D011487:Protein Conformation
chemical_list: D002843:Chromatin; D006657:Histones; D004247:DNA
vernacular_title: Bystryĭ metod polucheniia oktamera (H3-H4-H2A-H2B)(2) gistonov v bol'shikh kolichestvakh.