Prostaglandins in the kidney, urinary bladder and gills of the rainbow trout and European eel adapted to fresh water and seawater.
Prostaglandins in kidney, gills, and urinary bladder of freshwater-adapted and seawater-adapted rainbow trout, Oncorhynchus mykiss (= Salmo gairdneri), and European eel, Anguilla anguilla, were determined by solid-phase extraction of tissue homogenates and high-pressure liquid chromatography. Prostaglandins E2, E1, F1 alpha, F2 alpha, and D2 and the more stable metabolite of prostacyclin, 6-keto F1 alpha, occurred in these osmoregulatory tissues. In gill filaments and kidneys of both eel and trout, prostaglandins D2 and 6-keto F1 alpha were major prostaglandins. Concentrations of these prostaglandins were significantly lower in the eel after seawater adaptation, but not in the trout. The urinary bladder of the trout contained the highest levels of prostaglandins; bladders of seawater-adapted trout contained prostaglandin D2 at 6.7 ng/mg wet tissue, the highest level of any prostaglandin determined in the present studies. Prostaglandin D2 was not detected in bladders of freshwater-adapted trout.
Brown
J A
JA
Department of Biological Studies, Hatherly Laboratories, University of Exeter, United Kingdom.
Gray
C J
CJ
Hattersley
G
G
Robinson
J
J
D000222:Adaptation, Physiological / Q000502:physiology; D000811:Anguilla / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005618:Fresh Water; D005880:Gills / Q000737:chemistry*; D007668:Kidney / Q000737:chemistry*; D009928:Organ Specificity / Q000502:physiology; D011453:Prostaglandins / Q000032:analysis*; D012623:Seawater; D014337:Trout / Q000378:metabolism*; D001743:Urinary Bladder / Q000737:chemistry*
D011453:Prostaglandins