Comparative analysis of propionyl-CoA carboxylase from liver and mammary gland of mid-lactation cow.
1. Propionyl-CoA carboxylase from mid-lactation cow liver and mammary gland has been purified. 2. In both organs, the molecular mass was estimated to be approximately 450 kD, and two molecular subunits of 77 and 64 kD could be observed. 3. Physico-chemical and kinetical properties for the enzyme from both organs were similar, showing an allosteric behaviour in relation to ATP and Mg2+. 4. The presence of propionyl-CoA carboxylase in mid-lactation cow mammary gland with similar properties to the liver enzyme, could indicate the existence of a gluconeogenic metabolism in this organ exactly when a high demand of glucose for milk lactose is required.
Garrastazu
C
C
Departmento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Iniesta
M P
MP
Aranguez
M I
MI
Ruiz Amil
M
M
D000818:Animals; D002262:Carboxy-Lyases / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D005260:Female; D005943:Gluconeogenesis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007774:Lactation / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008321:Mammary Glands, Animal / Q000201:enzymology; D043586:Methylmalonyl-CoA Decarboxylase; D008970:Molecular Weight; D011247:Pregnancy; D011487:Protein Conformation; D014018:Tissue Distribution
D002262:Carboxy-Lyases; D043586:Methylmalonyl-CoA Decarboxylase