[An enzyme from the fungus Aspergillus niger that hydrolyzes choline ethers].
The acetylthiocholine-hydrolyzing enzymatic activity inhibited by the neostigmine and partly physostigmine has been found in extracts from mycelium of fungus Aspergillus niger. The enzyme has been isolated and 15-20 fold purified. The cholinesterase activity of the protein (Kmu 7.10-7 M) is comparable with known for analogous enzymes from higher plants, for its inhibition high concentrations of substrate (greater than 10-3M) are required. The enzyme hydrolyzes acetylthiocholine with rate approximately 1.5 times higher than butyrylthiocholine. Molecular mass of native protein is approximately 600 kDa, subunits -63 and 44 kDa.
Roshchina
V V
VV
Aleksandrova
I F
IF
D000109:Acetylcholine / Q000378:metabolism; D001234:Aspergillus niger / Q000201:enzymology*; D002794:Choline / Q000378:metabolism*; D002802:Cholinesterases / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008970:Molecular Weight
D002802:Cholinesterases; D002794:Choline; D000109:Acetylcholine
Ferment iz griba Aspergillus niger, gidrolizuiushchiĭ kholinovye éfiry.