Suppression of ethanolamine-containing glycerophospholipid synthesis in HL-60 cells during retinoic acid-induced differentiation.
Synthesis and degradation of glycerophospholipids in HL-60 cells and retinoic acid (RA)-treated HL-60 cells were examined. The synthesis of each subclass of ethanolamine-containing glycerophospholipids was extremely suppressed in RA-treated HL-60 cells, while that of other glycerophospholipids was not seriously affected. A pulse-chase experiment revealed that about 88% of 1,2-diacyl and 28% of 1-alkenyl-2-acyl glycerophosphoethanolamine were degraded during 4 days in RA-treated HL-60 cells. These characteristics of metabolism observed in RA-treated HL-60 cells might be responsible for the change of subclass composition of ethanolamine-containing glycerophospholipids in HL-60 cells during differentiation to granulocytes.
Naito
M
M
Faculty of Pharmaceutical Sciences, University of Tokyo.
Kudo
I
I
Nakagawa
Y
Y
Waku
K
K
Nojiri
H
H
Saito
M
M
Inoue
K
K
D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002794:Choline / Q000378:metabolism; D019856:Ethanolamine; D004983:Ethanolamines / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D015473:Leukemia, Promyelocytic, Acute; D010710:Phosphates / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000096:biosynthesis*; D010743:Phospholipids / Q000096:biosynthesis; D010761:Phosphorus Radioisotopes; D014212:Tretinoin / Q000494:pharmacology*; D014316:Tritium
D004983:Ethanolamines; D010710:Phosphates; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D010761:Phosphorus Radioisotopes; D014316:Tritium; D014212:Tretinoin; D019856:Ethanolamine; D002794:Choline