Immunoassay in the presence of water-soluble polymers.
To overcome the adverse consequences of antibody immobilization (impairment of the antigen-binding parameters) the interaction of a number of antigens with both soluble and covalently immobilized antibodies in the presence of water-soluble polymers has been investigated. Incorporation of some water-soluble polymers (Ficoll, Dextran) into the assay mixture was shown to substantially increase the antigen-binding capacity of both soluble and immobilized antibodies. The use of dextran T70 enhanced the sensitivity of competitive radioimmunoassays for CEA and beta 2-microglobulin 3-4-fold. This procedure may be also applied to shorten the incubation period when performing an assay.
Guzov
V M
VM
Institute of Bioorganic Chemistry, Byelorussian Academy of Sciences, Minsk, U.S.S.R.
Usanov
S A
SA
Chashchin
V L
VL
D000818:Animals; D000937:Antigen-Antibody Reactions; D002272:Carcinoembryonic Antigen / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D007163:Immunosorbent Techniques; D066298:In Vitro Techniques; D011108:Polymers; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D012995:Solubility; D014867:Water; D001613:beta 2-Microglobulin / Q000276:immunology
D002272:Carcinoembryonic Antigen; D011108:Polymers; D001613:beta 2-Microglobulin; D014867:Water