Two isoforms of the muscle acetylcholine receptor alpha-subunit are translated in the human cell line TE671.
We have previously reported the existence of 2 forms of mRNA for the human muscle acetylcholine receptor (AChR) alpha-subunit, thought to be generated by alternate splicing of a primary transcript and to encode 2 alpha-subunit protein isoforms. The 2 predicted alpha-subunit isoforms, differing by the insertion of 25 amino acids at position 58/59, have been synthesized from cRNA transcripts using rabbit reticulocyte lysates; these protein isoforms could be differentiated by immunoprecipitation using antibodies raised against synthetic peptides. The antibodies were used to demonstrate translation of both AChR alpha-subunit isoforms in the rhabdomyosarcoma (muscle) cell line TE671, in an approximate 1:1 ratio.
Morris
A
A
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
Beeson
D
D
Jacobson
L
L
Baggi
F
F
Vincent
A
A
Newsom-Davis
J
J
D000595:Amino Acid Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D005091:Exons; D006801:Humans; D007106:Immune Sera; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D014176:Protein Biosynthesis; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D011950:Receptors, Cholinergic / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D015183:Restriction Mapping
D007106:Immune Sera; D046911:Macromolecular Substances; D010455:Peptides; D012333:RNA, Messenger; D011950:Receptors, Cholinergic