Motoneuron survival in vitro: effects of pyruvate, alpha-ketoglutarate, gangliosides and potassium.
When grown in a serum and muscle extract containing medium, about 20% of chick motoneurons survived for one week. The presence of pyruvate during the first hour of culture doubles the number of motoneurons that survive. The subsequent addition of 5 mM alpha-ketoglutarate, 10-20 mM potassium, or 100 microM GM1 ganglioside results in a further increase in motoneurons surviving. These effects of alpha-ketoglutarate, potassium and GM1 ganglioside are not additive. Using such compounds in the culture medium, one can have a three-fold increase in the survival of motoneurons.
Juurlink
B H
BH
Department of Anatomy, University of Saskatchewan, Saskatoon, Canada.
Munoz
D G
DG
Ang
L C
LC
D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D002642:Chick Embryo; D005732:Gangliosides / Q000494:pharmacology; D007656:Ketoglutaric Acids / Q000494:pharmacology; D009046:Motor Neurons / Q000166:cytology / Q000187:drug effects*; D011188:Potassium / Q000494:pharmacology; D011773:Pyruvates / Q000494:pharmacology; D019289:Pyruvic Acid
D005732:Gangliosides; D007656:Ketoglutaric Acids; D011773:Pyruvates; D019289:Pyruvic Acid; D011188:Potassium