Use of PCR amplification for diagnosis of HTLV-I infection after blood transfusion.
We studied the seroconversion to human T-cell leukemia virus type-1 (HTLV-I) in two immunocompromised patients after transfusions of cellular blood components. One patient produced IgM antibodies against the viral p19 protein 149 days post-transfusion (a serum on day 43 was negative). Both patients showed indeterminate Western-blots (IgG anti-p19 and anti-gp46 but no anti-p24). Using the polymerase chain reaction (PCR) with two primer pairs (SK43/44 and SK54/56), we demonstrated HTLV-I infection prior to seroconversion. This infection was confirmed by Southern blot.
Agbalika
F
F
Department of Bacteriology-Virology, Hôpital Saint-Louis, Paris, France.
Honderlick
P
P
Ferchal
F
F
Perol
Y
Y
D000368:Aged; D015139:Blotting, Southern; D015153:Blotting, Western; D004277:DNA, Single-Stranded; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D015490:HTLV-I Infections / Q000175:diagnosis* / Q000209:etiology / Q000276:immunology; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D065227:Transfusion Reaction
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides