A sex-specific cytochrome P-450(F-1) colocalized with various neuropeptides in the paraventricular and supraoptic nuclei of female rats.
Numerous cells containing P-450(F-1) were detected in the magnocellular and parvocellular neurons of the paraventricular nucleus of the hypothalamus. Electron microscopic analysis of immunoreactive neurons has shown that P-450(F-1) immunoreactivity is present on the Golgi apparatus and rough endoplasmic reticulum. In the paraventricular nucleus, the P-450(F-1)-positive magnocellular neurons frequently contained oxytocin and some of them also contained CRF. Vasopressin was colocalized with P-450(F-1), but these neurons did not express CRF. In the supraoptic nucleus, P-450(F-1) was colocalized with oxytocin or CRF in single neurons, but not with vasopressin. No cells exhibiting the colocalization of both P-450(F-1) and somatostatin were observed in these nuclei. The results of the present study concerning colocalization of P-450 and peptides suggest that P-450(F-1) is involved in the hypothalamo-hypophyseal neuroendocrine function in the female rat.
Lin
L P
LP
Department of Neuroanatomy, Osaka University Medical School, Japan.
Lee
Y
Y
Tohyama
M
M
Shiosaka
S
S
D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000032:analysis; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005260:Female; D006056:Golgi Apparatus / Q000201:enzymology; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009479:Neuropeptides / Q000032:analysis*; D010121:Oxytocin / Q000032:analysis; D010286:Paraventricular Hypothalamic Nucleus / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D012727:Sex Characteristics; D013495:Supraoptic Nucleus / Q000201:enzymology*; D014667:Vasopressins / Q000032:analysis
D009479:Neuropeptides; D014667:Vasopressins; D010121:Oxytocin; D003346:Corticotropin-Releasing Hormone; D003577:Cytochrome P-450 Enzyme System