Rapid and sensitive discriminating determination of acetylcholinesterase activity in amniotic fluid with a choline sensor.
A simple method for the separate determination of acetylcholinesterase and butyrylcholinesterase activities in amniotic fluid is reported. This determination is performed with an enzyme electrode involving an immobilized choline oxidase membrane associated with the amperometric detection of hydrogen peroxide. Acetylcholine or butyrylcholine, in the presence of samples containing acetylcholinesterase or butyrylcholinesterase are specifically hydrolyzed, the formation of choline being detected vs time by the sensor with no need for a selective inhibitor. The dynamic linear ranges for acetylcholinesterase and butyrylcholinesterase are respectively 100 microU to 10 mU and 30 microU to 3 mU per ml sample.
Morelis
R M
RM
Laboratoire de Génie Enzymatique, Université Lyon, Villeurbanne, France.
Coulet
P R
PR
Simplot
A
A
Boisson
C
C
Guibaud
G
G
D000109:Acetylcholine / Q000378:metabolism; D000110:Acetylcholinesterase / Q000378:metabolism*; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000653:Amniotic Fluid / Q000201:enzymology*; D002091:Butyrylcholinesterase / Q000378:metabolism; D002794:Choline / Q000031:analogs & derivatives / Q000032:analysis* / Q000378:metabolism; D004566:Electrodes; D004800:Enzymes, Immobilized; D005260:Female; D006801:Humans; D008832:Microchemistry; D011247:Pregnancy
D004800:Enzymes, Immobilized; C017100:butyrylcholine; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D002794:Choline; D000109:Acetylcholine