Intraocular microdialysis with permanently implanted probes in rabbit.
The technique of microdialysis in vivo, much used in brain experiments, has been adapted for intraocular use. A new probe was designed, made from a soft tube with the dialysis membrane mounted in a fenestrated protecting sleeve, facing one side. A special surgical procedure was developed for the long-term implantation of the probes. They stay functional in the eyes for several weeks or more. Previously published intraocular microdialysis studies were short-term experiments. The acute surgical trauma is likely to affect the concentration of many compounds in the vitreous, and this effect is minimized with long-term probes.
Waga
J
J
Department of Ophthalmology, University of Lund, Sweden.
Ohta
A
A
Ehinger
B
B
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003956:Dialysis / Q000295:instrumentation / Q000379:methods*; D015314:Dialysis Solutions / Q000032:analysis; D004867:Equipment Design; D005123:Eye / Q000378:metabolism / Q000473:pathology; D010477:Perfusion; D011817:Rabbits; D014822:Vitreous Body / Q000378:metabolism*
D000596:Amino Acids; D015314:Dialysis Solutions