Reduction of rubella ELISA background using heat denatured sample buffer.
During the analysis of rubella-specific antibodies in sera from adult vaccinees, it was observed that the inclusion of heat-denatured blocking proteins in the sample dilution buffer substantially reduced non-specific binding of serum IgG to microtitre plates. Results suggest that this modification to standard ELISA technique may reduce the incidence of false positive results in these assays.
Mauracher
C A
CA
Department of Pediatrics, University of British Columbia, Vancouver, Canada.
Mitchell
L A
LA
Tingle
A J
AJ
D001426:Bacterial Proteins / Q000276:immunology; D002021:Buffers; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D011446:Prospective Studies; D011489:Protein Denaturation; D012409:Rubella / Q000276:immunology*; D012411:Rubella Vaccine / Q000276:immunology; D014611:Vaccination; D014765:Viral Vaccines / Q000276:immunology
D001426:Bacterial Proteins; D002021:Buffers; D007074:Immunoglobulin G; D012411:Rubella Vaccine; D014765:Viral Vaccines