Competitive direct enzyme-linked immunosorbent screening assay for the detection of sulfamethazine contamination of animal feeds.
A sensitive screening method has been developed for detecting sulfamethazine (SMZ) contamination of feeds by using either polyclonal or monoclonal antibodies and a direct competitive enzyme-linked immunosorbent screening assay (ELISA). Feed samples of 25.0 g are extracted with 0.5N HCl and centrifuged. The extract is adjusted to pH 7.0 with 3.0N NaOH and recentrifuged. This pH-adjusted extract is used in the ELISA. Levels as low as 0.004 micrograms SMZ/g feed were detected in supplemented extracts by polyclonal antibodies; levels of 0.4 micrograms SMZ/g feed were detected by a monoclonal antibody.
Dixon-Holland
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Rutgers University, Cook College, Department of Biochemistry and Microbiology, New Brunswick, NJ 08903-0231.
Katz
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D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D004350:Drug Residues / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis; D051379:Mice; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013418:Sulfamethazine / Q000032:analysis*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D013418:Sulfamethazine