High-performance liquid chromatographic evaluation of Med 15 and its metabolites Med 5 and tolmetin in rat plasma.
A simple and reliable high-performance liquid chromatographic method is described for the quantitative analysis of the new non-steroidal anti-inflammatory agent Med 15 and its metabolites Med 5 and tolmetin in rat plasma. After selective extraction the three analytes and an internal standard (p-phenyl-phenol) were separated on a reversed-phase Ultrasphere 5 micron column using potassium dihydrogenphosphate (0.05 M)-acetonitrile (52:48) (pH 4.7) as the mobile phase. The analytes were detected at 313 nm; the sensitivity of the method proved to be 0.05 microgram/ml for all three compounds. The method has been applied to investigate Med 15 pharmacokinetics in rats.
Mancinelli
A
A
Department of Drug Metabolism and Pharmacokinetics, Sigma-Tau, S.p.A., Rome, Italy.
Bruno
G
G
Cardace
G
G
Morabito
E
E
Marzo
A
A
Arrigoni Martelli
E
E
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D005998:Glycine / Q000031:analogs & derivatives* / Q000097:blood; D008297:Male; D013058:Mass Spectrometry; D011758:Pyrroles / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet; D014046:Tolmetin / Q000031:analogs & derivatives* / Q000097:blood*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D011758:Pyrroles; C065762:ST 679; C058077:tolmetin glycinamide; D014046:Tolmetin; D005998:Glycine