title: Effects of the prostaglandin analogue misoprostol on inflammatory mediator release by human monocytes.
abstract: The effect of misoprostol (M) on IL-1 beta, TNF-alpha, and lipid mediator release (assessed by RIA) by adherent (assessed by electron microscopy) human monocytes were studied in vitro. Human monocytes stimulated with E. Coli-derived lipopolysaccharide showed an increase in both IL-1 beta and TNF-alpha release. Incubation of the monocytes with LPS and M (18 hrs.), resulted in a reduction of both IL-1 beta and TNF-alpha levels. Leukotriene B4 levels did not increase in response to LPS or M. LPS also caused an increase in thromboxane (TXB2). M decreased TXB2 levels. 6-keto PGF1 alpha (6KP). Incubation with LPS and M stimulated release. LPS caused an increase in PGE2 levels. M (100 microM) caused an increase in PGE2 levels, M (1 microM) had no effect on PGE2. These data suggest a possible immunomodulatory role for misoprostol in inflammatory diseases.
authors.affiliation: Searle Research and Development, Skokie, IL 60077.
authors.forename: D L
authors.initials: DL
authors.lastname: Widomski
authors.forename: R E
authors.initials: RE
authors.lastname: Walsh
authors.forename: D A
authors.initials: DA
authors.lastname: Baron
authors.forename: M I
authors.initials: MI
authors.lastname: Hidvegi
authors.forename: D J
authors.initials: DJ
authors.lastname: Fretland
authors.forename: P W
authors.initials: PW
authors.lastname: Collins
authors.forename: T S
authors.initials: TS
authors.lastname: Gaginella
mesh_terms: D004926:Escherichia coli; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D008070:Lipopolysaccharides; D016595:Misoprostol / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D013268:Stimulation, Chemical
chemical_list: D008070:Lipopolysaccharides; D016595:Misoprostol