Stimulation of human fetal astrocyte proliferation by bacterial lipopolysaccharides and lipid A.
This report concerns the effect of bacterial endotoxin [lipopolysaccharide(LPS) and lipid A] on cultured human fetal astrocytes. Exposure to 1 micrograms/ml LPS or lipid A caused a striking stimulation of the rate of proliferation of the cells. The effect was most pronounced with exponentially growing cultures. Stimulation was associated with enhance DNA synthesis as ascertained by [3H]thymidine incorporation. These findings at the cellular level may be of relevance in the elucidation of the effects of bacterial endotoxins on the developing human brain.
Kato
M
M
Division of Child Neurology, Tottori University School of Medicine, Yonago, Japan.
Ohno
K
K
Takeshita
K
K
Herz
F
F
D001253:Astrocytes / Q000166:cytology* / Q000378:metabolism; D001345:Autoradiography; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004926:Escherichia coli; D005333:Fetus / Q000033:anatomy & histology* / Q000378:metabolism; D005625:Frontal Lobe / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D008050:Lipid A / Q000494:pharmacology*; D008070:Lipopolysaccharides; D009897:Optic Chiasm / Q000166:cytology; D012486:Salmonella typhimurium; D013936:Thymidine
D008050:Lipid A; D008070:Lipopolysaccharides; D004247:DNA; D013936:Thymidine