Light and electron microscopical localisation of 5-HT-immunoreactive boutons in the rat trigeminal motor nucleus.
We have used pre-embedding EM immunohistochemical methods to obtain quantitative data on the frequency and post-synaptic targets of 5-hydroxytryptamine-immunoreactive (5-HT-IR) boutons within the rat V motor nucleus. Thirteen percent (69/531) of all synaptic contacts in the motor nucleus involved 5-HT-IR boutons. Seventy-four percent of 5-HT-IR boutons made axo-dendritic contacts, 20% axo-somatic contacts, and 6% axo-axonic contacts. We conclude that a significant fraction of boutons in the motor nucleus are 5-HT-IR and most contribute to postsynaptic rather than presynaptic effects on trigeminal motoneurones.
Saha
S
S
Department of Physiology, University of Leeds, U.K.
Appenteng
K
K
Batten
T F
TF
D000818:Animals; D001369:Axons / Q000648:ultrastructure; D007150:Immunohistochemistry; D008853:Microscopy; D008854:Microscopy, Electron; D009046:Motor Neurons / Q000648:ultrastructure; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D013569:Synapses / Q000648:ultrastructure; D014278:Trigeminal Nuclei / Q000378:metabolism* / Q000648:ultrastructure
D012701:Serotonin