[Study of relations between the peripheral organ of olfaction and the olfactory bulb using anterograde and retrograde axon transport of horseradish peroxidase (HRP)].
Utilizing 26 Wistar albino rats, an anterograde and retrograde study has been performed on the connections between the first neuron of the olfactory pathway located in the olfactory epithelium and the second neuron in the glomeruli of the bulb. In the experiment we used horse radish peroxidase (HRP) in free form and combined with wheat lectin. In anterograde transport (epithelium-bulb), the HRP is deposited in preestablished sites in the olfactory epithelium of the nasal fossa. In retrograde transport (bulb-epithelium), HRP combined with wheat lectin is injected by means of a Hamilton microsyringe, glass micropipette and stereotaxic apparatus, in foreseen sites of the glomerular layer of the bulb.
Gil-Carcedo
L M
LM
Cátedra de Otorrinolaringología, Facultad de Medicina de Valladolid, Hospital Clínico Universitario.
Alonso
M V
MV
Gayoso
M
M
al-Majdalawi
A
A
D000818:Animals; D001369:Axons / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D009830:Olfactory Bulb / Q000378:metabolism*; D009831:Olfactory Mucosa / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012903:Smell
D006735:Horseradish Peroxidase
Estudio de las relaciones entre el órgano periférico de la olfación y el bulbo olfatorio, utilizando el transporte axónico anterógrado y retrógrado de la peroxidasa de rábano (HRP).