High-performance liquid chromatographic method for direct separation of 5-(p-hydroxyphenyl)-5-phenylhydantoin enantiomers using a chiral tris(4-methylbenzoate) column.
After simple purification of the incubation mixture of phenytoin in isolated rat hepatocytes, 5-(p-hydroxyphenyl)-5-phenylhydantoin (p-HPPH), which formed as a major metabolite, was readily resolved to each enantiomer by direct high-performance liquid chromatography on a cellulose tris(4-methylbenzoate) column, with a mobile phase of ethanol-water. It was also observed that the formation of S-(-)-p-HPPH was dominant, and the S/R ratio was 11.5.
Eto
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Department of Hospital Pharmacy, School of Medicine, University of Occupational and Environmental Health, Japan.
Noda
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Noda
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D000818:Animals; D001565:Benzoates; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008099:Liver / Q000737:chemistry / Q000166:cytology; D008297:Male; D010672:Phenytoin / Q000031:analogs & derivatives* / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D001565:Benzoates; C027364:5-(3-hydroxyphenyl)-5-phenylhydantoin; D010672:Phenytoin; C044605:methyl benzoate