[Through homologous recombination pathway recA gene takes part in chromosome replication of E. coli initiated by integrated plasmid].
We have reported in our previous paper that replication of E. coli chromosome initiated by the integrated F' plasmid depends on the recA gene. Here we report on work dealing with the role played by recA, recB, recC, lexA alleles in chromosome replication. Our results show that the recA gene takes part in chromosome replication through homologous recombination rather than SOS pathway and that Chi hot spot is not concerned with. They also show that the ATP-dependent dsDNA exonuclease activity of the RecBC enzyme has nothing to do with the recA gene in chromosome replication.
Mao
Y M
YM
Institute of Genetics, Fudan University, Shanghai.
Cheng
H P
HP
Huang
L
L
Sheng
Z J
ZJ
D001426:Bacterial Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D011938:Rec A Recombinases / Q000235:genetics*; D011995:Recombination, Genetic; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein
D001426:Bacterial Proteins; D011938:Rec A Recombinases; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein