Microbial metabolism of quinoline and related compounds. XII. Isolation and characterization of the quinoline oxidoreductase from Rhodococcus spec. B1 compared with the quinoline oxidoreductase from Pseudomonas putida 86.
Quinoline oxidoreductase from Rhodococcus spec. B1 was purified 39-fold to apparent homogeneity in a 5-step procedure with a recovery of 26%. The Mr of the native enzyme as determined by gel chromatography was 300,000. SDS polyacrylamide gel electrophoresis of the enzyme revealed 3 protein bands corresponding to Mr 82,000, 32,000, and 18,000. The enzyme contains 1.3 atoms of molybdenum, 8 atoms of iron, 8 atoms of acid-labile sulphur, 2 molecules of FAD and 2 molecules of molybdopterin cytosine dinucleotide. Cyanide, 4-hydroxymercuribenzoate and methanol were effective as inhibitors. The amino-terminal protein sequences of the 3 subunits of quinoline oxidoreductase from Rhodococcus B1 compared to those of quinoline oxidoreductase from Pseudomonas putida 86 revealed no difference among 71 amino acids examined.
Peschke
B
B
Institut für Mikrobiologie, Universität Hohenheim.
Lingens
F
F
D000595:Amino Acid Sequence; D002384:Catalysis; D007501:Iron / Q000032:analysis; D008969:Molecular Sequence Data; D008982:Molybdenum / Q000032:analysis; D010088:Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D016958:Pseudomonas putida / Q000201:enzymology* / Q000378:metabolism; D011804:Quinolines / Q000378:metabolism*; D012240:Rhodococcus / Q000201:enzymology* / Q000378:metabolism; D014871:Water Microbiology
D011804:Quinolines; D008982:Molybdenum; D007501:Iron; C037219:quinoline; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C068667:quinoline 2-oxidoreductase