Effect of phorbol ester on protein phosphorylation in the central nervous system of the leech Hirudo medicinalis: a two-dimensional electrophoretical analysis.
1. Proteins of different regions of the Hirudo medicinalis central nervous system have been analyzed by means of two-dimensional electrophoresis. 2. Subcellular distribution of phosphoproteins has been studied in leech segmental ganglia. 3. Phorbol 12,13-dibutyrate, a protein kinase C activator, stimulates the phosphorylation of a number of proteins whose isoelectric points and mol. wts are presented. 4. Putative roles for these phosphoproteins are discussed.
Garcia-Gil
M
M
Department of Physiology and Biochemistry, University of Pisa, Italy.
Bottai
D
D
Cannizzaro
M
M
Brunelli
M
M
D000818:Animals; D002490:Central Nervous System / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004789:Enzyme Activation; D005724:Ganglia / Q000378:metabolism; D007865:Leeches; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D011493:Protein Kinase C / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism
D011506:Proteins; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C