title: Multiple xylanases of Cellulomonas fimi are encoded by distinct genes.
abstract: Cellulomonas fimi genomic DNA encoding xylanase activity has been cloned and expressed in Escherichia coli. As judged by DNA hybridization and restriction analysis, twelve xylanase-positive clones carried a minimum of four different xylanase (xyn) genes. The encoded enzymes were devoid of cellulase activity but three of the four bound to Avicel.
authors.affiliation: Department of Biochemistry, AFRC Institute of Animal Physiology and Genetics Research, Cambridge, U.K.
authors.forename: J H
authors.initials: JH
authors.lastname: Clarke
authors.forename: J I
authors.initials: JI
authors.lastname: Laurie
authors.forename: H J
authors.initials: HJ
authors.lastname: Gilbert
authors.forename: G P
authors.initials: GP
authors.lastname: Hazlewood
mesh_terms: D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D015698:Genomic Library; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism; D006093:Gram-Positive Asporogenous Rods / Q000201:enzymology / Q000235:genetics*; D043325:Xylan Endo-1,3-beta-Xylosidase
chemical_list: D002482:Cellulose; D006026:Glycoside Hydrolases; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase