Measurement of alkaline phosphatase activity in serum with N-methyl-D-glucamine as a buffer: evaluation of the method for routine use.
Some aspects of the measurement of alkaline phosphatase activity concentration in human serum, using N-methyl-D-glucamine as a buffer, were evaluated with a view to the possible routine use of the method. The evaluated characteristics included: the temperature-dependence of the pH of the buffer; the effect of adding the magnesium/zinc ions buffer; the effect of the serum volume fraction; the substrate-starter versus the serum-starter mode; the effect of modifying the formulation of reagents; the within-run and the between laboratory imprecision; the correlation with an alternative routine method. Also, sex- and age-related reference values were produced, based on 2968 values from selected reference sample groups in 7 laboratories. In general, the results demonstrate the robustness of the method, its adaptability to a variety of mechanized analysers, and hence its feasibility as a routine measurement method.
Franzini
C
C
Ospedale di Rho, Milano, Italy.
Cattozzo
G
G
Ceriotti
F
F
Ferrero
C A
CA
Berti
P
P
Bianchi
P
P
Bonvicini
P
P
Ceriotti
G
G
Deledda
R
R
Fenili
D
D
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000469:Alkaline Phosphatase / Q000097:blood*; D002021:Buffers; D002648:Child; D002675:Child, Preschool; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D007231:Infant, Newborn; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008536:Meglumine; D008722:Methods; D008875:Middle Aged; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013379:Substrate Specificity; D013696:Temperature; D015032:Zinc / Q000494:pharmacology
D002021:Buffers; D008536:Meglumine; D000469:Alkaline Phosphatase; D008274:Magnesium; D015032:Zinc