Correlation between modification of membrane phospholipids and some biological activity of lymphocytes, neutrophils and macrophages.
Our study considered the possibility of modifying the functional response of human neutrophils, of mouse lymphocytes and macrophages treated with phospholipids having different polar groups, different isomerisms with saturated and unsaturated fatty acids from C12 to C20 carbon atoms. The results are as follows. a) Most of the phospholipids containing fatty acids from C12 to C20 cause inhibition of the blastogenic capacity of the polyclonal activators tested. b) The phospholipids tested cause a decrease in adherence of polymorphonuclear leukocytes with the exception of the phosphatidyl-choline containing saturated and unsaturated fatty acids. c) A decrease in polymorphonuclear leukocytes migrational capacity almost always occurs. d) The cells treated with L-phosphatidyl-ethanolamine having fatty acids from C14 to C17 show an increase in chemiluminescence; those treated with phosphatidyl-choline and L-phosphatidyl-glycerol show a decrease of the chemiluminescence; L-phosphatidic acid and L-phosphatidyl-ethanolamine having Microbial fatty acids (FAs) at C16 cause a decrease in the formation of phagolisosomes in the macrophages tested.
Galdiero
F
F
Istituto di Microbiologia I Facoltà di Medicina e Chirurgia, Università di Napoli, Italy.
Carratelli
C R
CR
Bentivoglio
C
C
Capasso
C
C
Cioffi
S
S
Folgore
A
A
Gorga
F
F
Ianniello
R
R
Mattera
S
S
Nuzzo
I
I
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects*; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D009504:Neutrophils / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects; D010743:Phospholipids / Q000737:chemistry / Q000494:pharmacology*
D008563:Membrane Lipids; D010743:Phospholipids