title: Recombinant hirudin: genetic engineering and structure analysis.
authors.affiliation: Ciba-Geigy Ltd., Biotechnology Department, Basel, Switzerland.
authors.forename: W E
authors.initials: WE
authors.lastname: Märki
authors.forename: H
authors.initials: H
authors.lastname: Grossenbacher
authors.forename: M G
authors.initials: MG
authors.lastname: Grütter
authors.forename: M H
authors.initials: MH
authors.lastname: Liersch
authors.forename: B
authors.initials: B
authors.lastname: Meyhack
authors.forename: J
authors.initials: J
authors.lastname: Heim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001412:Bacillus subtilis / Q000235:genetics; D001665:Binding Sites; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006629:Hirudins / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D007865:Leeches / Q000235:genetics; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013329:Structure-Activity Relationship; D013917:Thrombin / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D006629:Hirudins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; C072298:hirudin HV1; C072299:hirudin HV2; C072300:hirudin HV3; D004247:DNA; D013917:Thrombin; C060225:desirudin