title: Anomalous hypoxic acidification of medullary ventral surface.
abstract: In castrated male goats, two flexible catheters, one open ended for reference and the other ending in a 1-mm-diam glass bulb pH electrode, were advanced ventrally through a left posterior fossa craniotomy into the subarachnoid space between the 9th and 10th cranial nerve roots, passing medially into cerebrospinal fluid (CSF) over the medullary ventral surface (MVS). They were anchored to dura and fascia, tunneled under the scalp, and terminated in connectors on the left horn. After several days for recovery, while the animals were awake, the effects of CO2 and hypoxia on pH of the film of CSF between the pia and arachnoid (pHMVS) were recorded along with end-tidal PCO2 and PO2 (mass spectrometer), ventilation (pneumotachometer) through a permanent tracheostomy, and, when possible, ear arterial O2 saturation (SaO2). High PCO2 acidified MVS as expected: delta pH MVS/delta log PCO2. = -0.64 +/- 0.14, producing a ventilatory response slope delta VI/delta pHMVS = 372 l/min. Hypoxia resulted in acid shifts even when PCO2 was allowed to fall. The development of hypoxic acidosis was related to the location of pH electrodes determined at necropsy. In isocapnic hypoxia, pH over putative chemoreceptor surfaces fell in proportion to desaturation: delta pHMVS = 0.0033(SaO2)-0.34, r = 0.80, Sy.x = 0.025. With uncontrolled arterial PCO2, similar acidosis occurred when SaO2 fell below 85-90%: delta pHMVS = 0.0039(SaO2)-0.34, r = 0.88, Sy.x = 0.032. With constant hypoxia, pH fell (tau = 3.7 +/- 2.2 min) to a plateau after 10-20 min and showed rapid recovery (tau = 2.0 +/- 1.3 min).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Anesthesia, University of California, San Francisco 94143-0542.
authors.forename: F D
authors.initials: FD
authors.lastname: Xu
authors.forename: M J
authors.initials: MJ
authors.lastname: Spellman
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: J E
authors.initials: JE
authors.lastname: Baumgartner
authors.forename: S F
authors.initials: SF
authors.lastname: Ciricillo
authors.forename: J W
authors.initials: JW
authors.lastname: Severinghaus
mesh_terms: D000138:Acidosis / Q000209:etiology / Q000378:metabolism*; D000818:Animals; D002628:Chemoreceptor Cells / Q000187:drug effects / Q000378:metabolism; D004315:Doxapram / Q000494:pharmacology; D004566:Electrodes; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D006041:Goats; D006863:Hydrogen-Ion Concentration; D002534:Hypoxia, Brain / Q000150:complications / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008526:Medulla Oblongata / Q000378:metabolism*; D011149:Pons / Q000378:metabolism
chemical_list: D004315:Doxapram