Transforming growth factor-beta enhances secretory component and major histocompatibility complex class I antigen expression on rat IEC-6 intestinal epithelial cells.
Transforming growth factor-beta (TGF-beta) has been implicated as having a role in inflammatory responses by inducing cellular infiltration and the release of inflammatory cytokines. In this study, the IEC-6 rat intestinal epithelial cell line was used as a model to assess the effect of TGF-beta 1 on the expression of various plasma membrane determinants. TGF-beta 1 induced a dose-dependent increase in the percentage of cells expressing surface secretory component (SC) and class I major histocompatibility (MHC) antigens. However, the expression of class II MHC was unaffected. In contrast, epidermal growth factor had no effect on any of the surface proteins studied. The TGF-beta 1-enhanced expression of SC was accompanied by an enhanced binding of polymeric, but not monomeric, immunoglobulin A (IgA). Preincubation of the TGF-beta 1-treated cells with an anti-human beta-galactosyltransferase (beta-GT) antiserum did not block the binding of the anti-SC antibody, indicating that the TGF-beta-induced increase in SC staining was due to SC expression and not the polymeric immunoglobulin-binding enzyme, beta-GT. These results indicate that TGF-beta 1 may be important in immune functions involving intestinal epithelial cells by enhancing the expression of surface class I MHC antigens and SC, a protein responsible for the transport of polymeric IgA into the intestinal lumen.
McGee
D W
DW
Department of Microbiology, University of Alabama, Birmingham 35294.
Aicher
W K
WK
Eldridge
J H
JH
Peppard
J V
JV
Mestecky
J
J
McGhee
J R
JR
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000276:immunology; D005700:Galactosyltransferases / Q000276:immunology; D005786:Gene Expression Regulation / Q000187:drug effects; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis*; D007070:Immunoglobulin A / Q000276:immunology; D007249:Inflammation / Q000276:immunology; D007422:Intestines / Q000187:drug effects* / Q000276:immunology; D008565:Membrane Proteins / Q000096:biosynthesis; D051381:Rats; D012635:Secretory Component / Q000096:biosynthesis*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D015395:Histocompatibility Antigens Class I; D007070:Immunoglobulin A; D008565:Membrane Proteins; D012635:Secretory Component; D016212:Transforming Growth Factor beta; D004815:Epidermal Growth Factor; D005700:Galactosyltransferases