Serum concentrations of intact type IV collagen in diabetics.
We developed a sandwich enzyme immunoassay for intact type IV collagen (IV-C) using two monoclonal antibodies as a marker of basement membrane metabolism, and examined the relationship between serum IV-C and diabetic complications. Serum IV-C was measured in 186 diabetics with or without clinical signs of retinopathy, neuropathy, and/or nephropathy, and 328 healthy subjects. Serum IV-C was significantly (P less than .01) higher in diabetics (mean +/- SE: 124.83 +/- 2.94 ng/ml) than in healthy subjects (73.32 +/- 1.42 ng/ml). In diabetics with microangiopathy, serum IV-C became higher as clinical signs worsened. Especially in diabetics with nephropathy, serum IV-C became higher with the elevation of blood urea nitrogen (BUN), serum creatinine, and beta 2-microglobulin. Serum IV-C level seems to be a useful marker for assessment of the activity or progression of diabetic microangiopathy, and for evaluation of therapeutic effects.
Matsumoto
E
E
First Department of Medicine, Wakayama University of Medical Science, Japan.
Matsumoto
G
G
Bessho
H
H
Kikuoka
H
H
Nanjo
K
K
D000419:Albuminuria; D000911:Antibodies, Monoclonal; D001485:Basement Membrane / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D003094:Collagen / Q000097:blood*; D003920:Diabetes Mellitus / Q000097:blood* / Q000503:physiopathology; D003928:Diabetic Nephropathies / Q000097:blood / Q000175:diagnosis; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007677:Kidney Function Tests; D008297:Male; D008875:Middle Aged; D011507:Proteinuria; D012016:Reference Values
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D003094:Collagen