Perfluorochemical emulsion effect on human albumin binding of valproic acid.
At 37 degrees C, valproic acid was weakly bound by a PFCE through an interaction with the emulsifiers that was independent of both buffer and PFCE concentration. The binding by PFCE was dependent on valproic acid concentration in 0.1 M buffer, but not in 0.2 M buffer. The addition of PFCE to 4% HSA increased the percent free valproic acid due to both HSA dilution and displacement of HSA bound drug. This displacement was apparently due to an interaction with the PFC liquids and/or intact PFCE droplets, and with the oleic acid component of the PFCE.
Parsons
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DL
Department of Pharmacal Sciences, School of Pharmacy, Auburn University, AL 36849.
Ravis
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WR
Huang
H C
HC
D001665:Binding Sites; D002021:Buffers; D004338:Drug Combinations; D005466:Fluorocarbons / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives; D019301:Oleic Acid; D009829:Oleic Acids / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D014635:Valproic Acid / Q000378:metabolism*
D002021:Buffers; D004338:Drug Combinations; D005466:Fluorocarbons; D006895:Hydroxyethyl Starch Derivatives; D009829:Oleic Acids; D010743:Phospholipids; D012709:Serum Albumin; D019301:Oleic Acid; D014635:Valproic Acid; C017323:glucose, glycerol, hydroxyethyl starch, perfluorodecalin, perfluorotripropylamine, pluronic F-68, salts, yolk phospholipids drug combination