A comparison of the direct immunobead test and other tests for sperm antibodies detection.
The authors report the results of a correlation study between the direct Immunobead Test (d-IBT) and other techniques for antisperm antibodies detection. The Gelatin Agglutination Test (GAT) and Tray Agglutination Test (TAT) were used to detect antibodies in blood serum and seminal plasma ("indirect methods"). The Direct IgG Mixed Antiglobulin Reaction Test (d-MAR test) was used to detect sperm antibodies bound to the sperm surface ("direct method"). A good concordance between the methods, measured by phi and K tests, was found and satisfactory mathematical models were established by regression analyses.
Dondero
F
F
Laboratorio di Immunologia della Riproduzione e Seminologia, V Clinica Medica, Università di Roma, La Sapienza, Italy.
Lenzi
A
A
Gandini
L
L
Lombardo
F
F
Culasso
F
F
D000372:Agglutination Tests / Q000379:methods*; D000704:Analysis of Variance; D001323:Autoantibodies / Q000032:analysis*; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007248:Infertility, Male / Q000175:diagnosis / Q000276:immunology; D008297:Male; D015233:Models, Statistical; D012044:Regression Analysis; D012661:Semen / Q000276:immunology; D012680:Sensitivity and Specificity; D013094:Spermatozoa / Q000276:immunology*
D001323:Autoantibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M