title: [Immunoenzyme detection of the mycotoxin, ochratoxin A].
abstract: Indirect, enzymoimmunological assays of the mycotoxin ochratoxin A were developed. In this technique a polyclonal (rabbit) antibody to ochratoxin A was used, along with the other, peroxidase-labelled (pig anti rabbit) antibody. The sensitivity of this method ranged around 75 pg of ochratoxin A per pit. The range of calibration curve was from 10 to 1000 pg per pit. The cross reactions with other ochratoxins made 1.4% (ochratoxin C). The ELISA test of ochratoxin A can be used as an expeditious screening method for a preliminary examination of the greater number of samples.
authors.affiliation: Institut hygieny a epidemiologie, Brno.
authors.forename: J
authors.initials: J
authors.lastname: Ruprich
authors.forename: V
authors.initials: V
authors.lastname: Ostrý
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D009793:Ochratoxins / Q000032:analysis*
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A
vernacular_title: Enzymoimunologické stanovení mykotoxinu ochratoxin A.