title: Interference of digoxin-like immunoreactive substances with TDx digoxin II assay in different patients.
abstract: The TDx digoxin II immunoassay was used in controls and in patients not taking digoxin or any other cardiac glycoside. We report the frequency of interference thought to be caused by digoxin-like factors (DLFs). We found a high incidence of false-positive results in 15 patients with severe hepatic disease (60% false-positive results). In newborn infants we found false-positive results both when their blood was drawn from a peripheral vein (89% false-positive results) and, more strikingly (100% false-positive results), when it was obtained from an umbilical cord vein (p less than 0.001). Compared to a control group, no statistically significant false-positive results were found in patients with mild to moderate chronic renal failure (n = 21) or in pregnant women (n = 15). In patients with chronic renal failure undergoing hemodialysis six of 25 had false-positive results. These results suggest that digoxin levels must be interpreted carefully in patients with chronic liver disease and chronic renal failure and in newborns until new methods that eliminate the interference caused by DLFs become readily available.
authors.affiliation: Clinical Pharmacology Service, Hospital Marques de Valdecilla, Santander, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Avendaño
authors.forename: J S
authors.initials: JS
authors.lastname: Alvarez
authors.forename: J A
authors.initials: JA
authors.lastname: Sacristan
authors.forename: J
authors.initials: J
authors.lastname: Adin
authors.forename: M J
authors.initials: MJ
authors.lastname: Alsar
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000032:analysis*; D002298:Cardenolides; D004077:Digoxin / Q000097:blood*; D005188:False Negative Reactions; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D016231:Fluorescence Polarization Immunoassay / Q000379:methods; D006801:Humans; D007231:Infant, Newborn; D007676:Kidney Failure, Chronic / Q000097:blood; D008103:Liver Cirrhosis / Q000097:blood; D011247:Pregnancy / Q000097:blood; D011446:Prospective Studies; D006435:Renal Dialysis; D012503:Saponins
chemical_list: D001798:Blood Proteins; D002298:Cardenolides; D012503:Saponins; C034504:digoxin-like factors; D004077:Digoxin