Retinol uptake and metabolism to 3,4-didehydroretinol in human keratinocytes at various stages of differentiation.
The concentration of 3,4-didehydroretinol, an epidermal metabolite of retinol, is changed both in association with keratinizing disorders and during treatment with certain synthetic retinoids. To delineate further the factors regulation biosynthesis of 3,4-didehydroretinol, keratinocytes from three different layers of normal epidermis were prepared by trypsinization and incubated with [3H]-retinol for 20 h. Cell-bound [3H]-retinol and 3,4-[3H]-didehydroretinol were separated by high-performance liquid chromatography. The results indicate that the uptake of retinol and the synthesis of 3,4-didehydroretinol occur predominantly in undifferentiated cells from the basal and spinous layers of the epidermis.
Törmä
H
H
Department of Dermatology, University Hospital, Linköping, Sweden.
Vahlquist
A
A
D002454:Cell Differentiation; D002851:Chromatography, High Pressure Liquid; D000078404:Epidermal Cells; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000378:metabolism*
C024366:vitamin A2; D014801:Vitamin A