Atomic force microscopy of supported planar membrane bilayers.
Membrane bilayers of dipalmitoyl phosphatidylcholine (DPPC) and dipalmitoyl phosphatidylethanolamine (DPPE) adsorbed to a freshly cleaved mica substrate have been imaged by Atomic Force Microscopy (AFM). The membranes were mounted for imaging by two methods: (a) by dialysis of a detergent solution of the lipid in the presence of the substrate material, and (b) by adsorption of lipid vesicles onto the substrate surface from a vesicle suspension. The images were taken in air, and show lipid bilayers adhering to the surface either in isolated patches or in continuous sheets, depending on the deposition conditions. Epifluorescence light-microscopy shows that the lipid is distributed on the substrate surfaces as seen in the AFM images. In some instances, when DPPE was used, whole, unfused vesicles, which were bound to the substrate, could be imaged by the AFM. Such membranes should be capable of acting as natural anchors for imaging membrane proteins by AFM.
Singh
S
S
Department of Chemistry, University of New Mexico, Albuquerque 87131.
Keller
D J
DJ
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D007202:Indicators and Reagents; D008051:Lipid Bilayers / Q000737:chemistry*; D008854:Microscopy, Electron / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D008968:Molecular Conformation; D010714:Phosphatidylethanolamines / Q000737:chemistry*
D007202:Indicators and Reagents; D008051:Lipid Bilayers; D010714:Phosphatidylethanolamines; D015060:1,2-Dipalmitoylphosphatidylcholine; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine