Effects of gamma-rays and glucose analogs on the energy metabolism of a cell line derived from human cerebral glioma.
Effects of gamma-rays and glucose analogs, 2-deoxy-D-glucose (2-DG), 5-thio-D-glucose (5-TG) and 3-O-methyl glucose (3-O-MG) on cellular energy metabolism have been studied in a cell line, derived from a human cerebral glioma, by analysing intermediates of glycolysis and some important nucleotides (ATP, NAD etc.) using the technique of isotachophoresis. Gamma-irradiation induced a transient decrease in the nucleotide levels accompanied by an accumulation of sugar phosphates, the nucleotide levels recovering in a few hours post-irradiation. 2-DG inhibited glycolysis and reduced the nucleotide levels of irradiated as well as unirradiated cells in a concentration-dependent manner both in presence and absence of respiration, whereas 5-TG and 3-O-MG did not show significant effects in the presence of respiration. Reduced energy status observed with 2-DG under respiratory proficient conditions was completely reversed in 2 hr following its removal, whereas such a recovery was not observed in the absence of respiration. These results have important implications in the energy-linked modifications of tumour radiation response using glucose analogs.
Dwarakanath
B S
BS
Department of Biophysics, National Institute of Mental Health and Neurosciences, Bangalore.
Jain
V
V
D019325:3-O-Methylglucose; D000227:Adenine Nucleotides / Q000378:metabolism*; D001932:Brain Neoplasms; D002460:Cell Line; D003847:Deoxyglucose / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects / Q000528:radiation effects*; D005720:Gamma Rays; D005910:Glioma; D005947:Glucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D008757:Methylglucosides / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*
D000227:Adenine Nucleotides; D008757:Methylglucosides; D011838:Radiation-Sensitizing Agents; D019325:3-O-Methylglucose; C003002:5-thio-D-glucose; D003847:Deoxyglucose; D005947:Glucose