title: Effects of a low dietary linoleic acid level on intestinal morphology and enterocyte brush border membrane lipid composition.
abstract: The influence of low dietary linoleic acid level (an essential fatty acid deficiency) on the intestine mucosal morphology and the purified brush border membrane (BBM) lipid composition was investigated in the rat. Electron micrographs and morphometric measurements showed that villi and crypt sizes as well as the ultrastructure of epithelial cells were altered. Cholesterol (CHOL) and phospholipid (PL) levels, CHOL/PL ratio and PL class distribution were not changed by the low linoleate diet. However, the fatty acid composition of phospholipids was markedly modified in the enterocyte BBM, showing elevated amounts of palmitoleic (16:1n-7), oleic (18:1n-9) and 5,8,11-eicosatrienoic (20:3n-9) acids and, by contrast, depressed linoleic (18:2n-6) and arachidonic (20:4n-6) acid levels. Although the underlying mechanisms remain unknown the results obtained suggest that essential fatty acids (EFA) could be directly involved in the trigger action of the observed alterations, as regards both their dynamic (metabolic) and structural roles.
authors.affiliation: INRA, Laboratoire de Nutrition et Sécurité Alimentaire, Jouy-en-Josas, France.
authors.forename: R
authors.initials: R
authors.lastname: Christon
authors.forename: J C
authors.initials: JC
authors.lastname: Meslin
authors.forename: J
authors.initials: J
authors.lastname: Thévenoux
authors.forename: A
authors.initials: A
authors.lastname: Linard
authors.forename: C L
authors.initials: CL
authors.lastname: Léger
authors.forename: S
authors.initials: S
authors.lastname: Delpal
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage*; D005228:Fatty Acids, Essential / Q000172:deficiency; D005260:Female; D007082:Ileum / Q000648:ultrastructure; D007413:Intestinal Mucosa / Q000648:ultrastructure; D007422:Intestines / Q000648:ultrastructure*; D007583:Jejunum / Q000648:ultrastructure; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000008:administration & dosage*; D008563:Membrane Lipids / Q000378:metabolism*; D008854:Microscopy, Electron; D008871:Microvilli / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004041:Dietary Fats; D005228:Fatty Acids, Essential; D008041:Linoleic Acids; D008563:Membrane Lipids; D010743:Phospholipids; D002784:Cholesterol; D019787:Linoleic Acid