title: Preparation of a drug-induced allergic hepatic disorder model with penicillin as hapten.
abstract: A drug-induced allergic hepatic disorder model was established using a hapten and carrier. Penicillin G was bound to glycine for the preparation of N-hydroxy succinic imidylglycinyl benzylpenicillate (PG-Gly-OSu). Using this as the hapten and liver protein as the carrier, guinea pigs were sensitized with liver protein bound to PG-Gly-OSu. After 2 weeks, the sensitized guinea pigs were directly challenged with hepatocytes bound to PG-Gly-OSu through a mesenteric vein and hepatocellular disorder was induced. When the sensitized guinea pigs were challenged with PG-Gly-OSu alone or with liver protein alone, hepatocellular disorder could not be induced. These results suggest that the combination of PG-Gly-OSu as the hapten and liver protein as the carrier elicits a hepatocellular disorder similar to drug-induced allergic hepatitis.
authors.affiliation: Third Department of Internal Medicine, Osaka City University Medical School, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Mizoguchi
authors.forename: M
authors.initials: M
authors.lastname: Iwasaki
authors.forename: H
authors.initials: H
authors.lastname: Tsutsui
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Monna
authors.forename: S
authors.initials: S
authors.lastname: Morisawa
mesh_terms: D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000276:immunology / Q000473:pathology*; D004195:Disease Models, Animal; D004342:Drug Hypersensitivity / Q000276:immunology / Q000473:pathology*; D006168:Guinea Pigs; D006241:Haptens / Q000009:adverse effects* / Q000378:metabolism; D006520:Hepatitis, Animal / Q000139:chemically induced* / Q000276:immunology / Q000473:pathology; D008297:Male; D010406:Penicillins / Q000009:adverse effects* / Q000378:metabolism
chemical_list: D006241:Haptens; D010406:Penicillins