title: Metabolic fates of L-tryptophan in Saccharomyces uvarum (Saccharomyces carlsbergensis).
abstract: The metabolism of L-tryptophan by Saccharomyces uvarum (carlsbergensis) was investigated by simultaneous measuring of fluxes through kynureninase, through transaminases and into protein using L-[methylene-14C] and L-[side chain-2,3-3H]tryptophan. In yeasts cultivated in synthetic medium (S medium), the flux into protein was predominant, closely followed by the flux leading to 2-3H liberation. The proportion of L-tryptophan metabolized via the latter flux increased over 10-fold (75% of total tryptophan metabolized) as the concentration of L-tryptophan was raised from 5 x 10(-5) to 5 x 10(-4) M. L-Tryptophan metabolized via the kynureninase flux was less than 5% of total tryptophan metabolized. In yeast extract-polypepton-glucose medium (YPG medium), more tryptophan was incorporated into protein than in the S medium. Contribution of the kynureninase flux remained very low. Tryptophan metabolism via each flux changed depending on the growth phase. 2-3H liberation was shown to be primarily due to tryptophol synthesis by high performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR), indole-3-acetic acid and kynurenic acid also contributing to 2-3H liberation but to a much lesser extent. 2-3H liberation increased dose-dependently at tryptophan concentration higher than 10(-5)M, while the kynureninase flux reached its plateau at 10(-5)M. Formation of tryptophol and indole-3-acetic acid via indole-3-pyruvic acid and indole-3-acetaldehyde with indole aldehyde as a by-product was confirmed using exogenous tryptophan metabolites with indole rings.
authors.affiliation: School of Pharmacy, Kobe-Gakuin University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shin
authors.forename: T
authors.initials: T
authors.lastname: Shinguu
authors.forename: K
authors.initials: K
authors.lastname: Sano
authors.forename: C
authors.initials: C
authors.lastname: Umezawa
mesh_terms: D001711:Biotransformation; D012440:Saccharomyces / Q000378:metabolism*; D014364:Tryptophan / Q000378:metabolism*
chemical_list: D014364:Tryptophan