Increased binding of thiopental in tissue homogenates of the rat in the presence of a volatile anesthetic.
Thiopental (CAS 76-75-5) binding (0.4 mmol.l-1) in tissue homogenate of rats (liver, brain, heart, kidney, lung, spleen and skeletal muscle) was studied by equilibrium dialysis. Percentage of thiopental bound was relatively low in homogenate of brain, lung, spleen and skeletal muscle (14-19%); it was much higher in that of liver, heart and kidney (24-27%). Simultaneously present halothane (11.8 mmol.l-1) increased the percentage of thiopental bound in the homogenate of all tissues investigated at least to a factor of 1.4 (spleen) and maximally of 2.4 (brain). The same phenomenon of an increased thiopental binding in tissue homogenate was found in the presence of 10.3 mmol.l-1 enflurane (except skeletal muscle) and 10.2 mmol.l-1 isoflurane (except kidney, spleen and skeletal muscle), yet to a significantly lower extent in the presence of these halogenated ethers as compared with halothane.
Büch
U
U
Clinic of Anesthesiology and Intensive Care Medicine, University of the Saarland, Fed. Rep. of Germany.
Altmayer
P
P
Isenberg
J C
JC
Büch
H P
HP
D000777:Anesthetics / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003956:Dialysis; D004737:Enflurane / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D006221:Halothane / Q000737:chemistry / Q000494:pharmacology; D007530:Isoflurane / Q000737:chemistry / Q000494:pharmacology; D008970:Molecular Weight; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D013874:Thiopental / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D000777:Anesthetics; D004737:Enflurane; D007530:Isoflurane; D013874:Thiopental; D006221:Halothane