[Effects of EGF and retinoic acid on differentiation of squamous cell carcinoma cells].
Using monoclonal antibody, G6K12, a novel differentiation marker of keratinocyte, we examined the effect of EGF and retinoic acid on the differentiation of SCC-25, a cell line established from human squamous cell carcinoma. EGF inhibited the expression of cell-surface differentiation marker in low cell-density, but not in high cell-density. Retinoic acid (more than 10(-8) M) inhibited the expression of the antigens. The terminal differentiation of SCC-25 cells occurred at the condition of high cell density. These results obtained demonstrate that EGF and retinoic acid inhibit the terminal differentiation of SCC-25 cells, and that the cell density is the important factor for the commitment of the terminal differentiation.
Harada
H
H
First Department of Oral Surgery, Faculty of Dentistry, Kyushu University, Fukuoka.
Tashiro
H
H
Kukita
T
T
Kurisu
K
K
D002294:Carcinoma, Squamous Cell / Q000503:physiopathology*; D002454:Cell Differentiation / Q000187:drug effects; D003864:Depression, Chemical; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006801:Humans; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D014212:Tretinoin; D004815:Epidermal Growth Factor