Three types of mouse peptidylarginine deiminase: characterization and tissue distribution.
Three types of mouse peptidylarginine deiminase were separated by DEAE-Sephacel ion-exchange column chromatography, and we propose designating them peptidylarginine deiminase type I, II, and III according to the order of elution. The type II enzyme was widely distributed in various tissues including the skeletal muscle, whereas the type I enzyme was localized in the epidermis and uterus, and the type III enzyme was detected in the epidermis and hair follicles. These enzymes were distinguished by their molecular weights and substrate specificity. The molecular weights were estimated to be approximately 54,000 (type I) and 100,000 (type II and III) by Sephacryl S-200 gel filtration column chromatography. On SDS-PAGE the type II and III enzymes gave Mr = 81,000 and Mr = 76,000, respectively. Among the substrates tested, the type I enzyme showed highest activity toward BZ-L-Arg-NH2, type II toward BZ-L-Arg-O-Et, and type III toward protamine. Western blot analysis showed that antibodies against the type II enzyme were immuno-crossreactive to the type III enzyme.
Terakawa
H
H
Laboratory of Biochemistry, Faculty of Agriculture, Ibaraki University.
Takahara
H
H
Sugawara
K
K
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D004229:Dithiothreitol / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006867:Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D005779:Immunodiffusion; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D009132:Muscles / Q000201:enzymology; D009928:Organ Specificity; D010179:Pancreas / Q000201:enzymology; D000080002:Protein-Arginine Deiminase Type 4; D000076342:Protein-Arginine Deiminases; D012469:Salivary Glands / Q000201:enzymology; D012867:Skin / Q000166:cytology / Q000201:enzymology; D013116:Spinal Cord / Q000201:enzymology; D013379:Substrate Specificity; D014599:Uterus / Q000201:enzymology
D007527:Isoenzymes; D006867:Hydrolases; D000080002:Protein-Arginine Deiminase Type 4; D000076342:Protein-Arginine Deiminases; C552151:peptidylarginine deiminase 4, mouse; D002118:Calcium; D004229:Dithiothreitol