[Study on serum-sensitivity of Pseudomonas aeruginosa].
Using a microtiter plate for cell culture one hundred strains of Pseudomonas aeruginosa isolated from various clinical materials were quantitatively investigated for sensitivity to the bactericidal activity of normal human serum. The sensitivity to the serum was widely distributed among these strains. In parallel, 100 strains of Pseudomonas aeruginosa were serotyped using a type-specific anti-Pseudomonas antiserum kit. All type M strains were proven to be serum-sensitive, while types G, B, and A were generally serum-resistant. Using an experimental model of osteomyelitis in mice, osteomyelitis could not be caused by serum-sensitive strains of Pseudomonas aeruginosa. These results would indicate that serum-resistance is an important factor in pathogenicity of Pseudomonas aeruginosa.
Shirahige
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Department of Orthopaedic Surgery, Jikei University, School of Medicine, Tokyo, Japan.
D000328:Adult; D000818:Animals; D001769:Blood / Q000276:immunology*; D001770:Blood Bactericidal Activity; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D010019:Osteomyelitis / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000276:immunology* / Q000472:pathogenicity; D012703:Serotyping