title: A chemiluminescent immunoassay for urinary thromboxane B2.
abstract: Because of the vasoactive properties of thromboxane A2 and other related prostaglandins, much research has been conducted on drugs which alter their levels. Urinary levels of thromboxane B2 and 2,3-dinor thromboxane B2 (major urinary metabolite of thromboxane B2) are used as an indication of thromboxane production in-vivo. In order to accurately measure urinary TXB2 levels of subjects on investigative drugs which lower TXA2 and subsequently TXB2, a simple and sensitive analytical tool becomes necessary. We have thus developed a non-radioisotopic (chemiluminescent) assay for urinary TXB2. Sensitivity has been demonstrated to 5 pg/ml. The method correlates well with gas chromatography/mass spectrometry (the accepted reference method) even without column chromatographic purification prior to the conduct of the chemiluminescent assay (r = 0.96). In addition, we have demonstrated feasibility for a chemiluminescent assay to measure urinary 2,3-dinor TXB2.
authors.affiliation: Ciba Corning Diagnostics, Inc. E. Walpole, MA 02032.
authors.forename: A
authors.initials: A
authors.lastname: Morello
authors.forename: S
authors.initials: S
authors.lastname: Chang
authors.forename: A
authors.initials: A
authors.lastname: Jacob
authors.forename: S J
authors.initials: SJ
authors.lastname: Law
authors.forename: M
authors.initials: M
authors.lastname: Stastny
authors.forename: L
authors.initials: L
authors.lastname: Martin
authors.forename: A
authors.initials: A
authors.lastname: Pamidi
authors.forename: A
authors.initials: A
authors.lastname: Kotake
mesh_terms: D000166:Acridines / Q000138:chemical synthesis; D002849:Chromatography, Gas; D003429:Cross Reactions; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements; D013058:Mass Spectrometry; D015233:Models, Statistical; D013929:Thromboxane B2 / Q000031:analogs & derivatives / Q000737:chemistry / Q000652:urine*
chemical_list: D000166:Acridines; D013929:Thromboxane B2; C028965:2,3-dinor-thromboxane B2