Time window analysis and sorting.
Flow cytometric hardware and procedures were developed to continuously analyse and to sort a particular time window in a kinetic response. The technique uses balanced air pressure to drive a stimulus-bead mixture from a vial to a t-junction where it mixes passively with cells. The t-junction is distanced from the flow cell and air pressure regulated so that the stimulation occurs at a fixed and adjustable time before the cells are interrogated by the laser beam. Practical applications of the device demonstrate utility with cells whose responses are seen in seconds or minutes. The device is easily implemented on any sorting flow cytometer.
Dunne
J F
JF
Syntex Research, Palo Alto, California 94304.
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000378:metabolism; D001920:Bradykinin / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002461:Cell Line, Transformed / Q000502:physiology; D004867:Equipment Design; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D017022:Flow Injection Analysis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007834:Lasers; D051379:Mice; D051381:Rats; D015398:Signal Transduction
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D001920:Bradykinin; D002118:Calcium