Template-directed extension of a guanosine 5'-phosphate covalently attached to an oligodeoxycytidylate template.
We have prepared molecules in which a guanosine 5'-phosphate (pG) residue is attached to the 3' terminus of a decadeoxycytidylate (pdC)10 template via diamine linkers H2N(CH2)nNH2, n = 4-7. The pG residue acts as a primer and is extended very efficiently by incubation with activated pG derivatives to give products containing 6-9 G residues in greater than 80% yield. The detailed nature of the product distribution is discussed.
Rodriguez
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Salk Institute for Biological Studies, San Diego, CA 92186-5800.
Orgel
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D001483:Base Sequence; D003568:Cytidine Monophosphate / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006150:Guanine Nucleotides / Q000737:chemistry*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013698:Templates, Genetic
D006150:Guanine Nucleotides; D009838:Oligodeoxyribonucleotides; D003568:Cytidine Monophosphate