title: A C-terminal proline is required for bioluminescence of the Ca(2+)-binding photoprotein, aequorin.
abstract: The requirement for a proline residue at the C-terminus of the Ca(2+)-binding photoprotein, aequorin, was investigated by measuring luminescence activities of a series of C-terminal deletion mutants, substitution mutants and an addition mutant. CD spectral measurements of apoaequorin with the C-terminal proline deleted showed a small change in secondary structure. In all cases studied, the C-terminal proline was required for bioluminescence activity.
authors.affiliation: Osaka Bioscience Institute, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nomura
authors.forename: S
authors.initials: S
authors.lastname: Inouye
authors.forename: Y
authors.initials: Y
authors.lastname: Ohmiya
authors.forename: F I
authors.initials: FI
authors.lastname: Tsuji
mesh_terms: D000331:Aequorin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002942:Circular Dichroism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D049449:Luminescence; D008969:Molecular Sequence Data; D011392:Proline; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D015183:Restriction Mapping; D037401:Scyphozoa
chemical_list: D011994:Recombinant Proteins; D000331:Aequorin; D011392:Proline