title: Effect of pulse amplitude of luteinizing hormone, duration and rate of change on progesterone secretion from rat corpora lutea.
abstract: Corpora lutea (CL) were obtained from immature rats primed with pregnant mares' serum gonadotrophin followed by human chorionic gonadotrophin (hCG). Two days after hCG, CL were isolated, placed in perifusion culture and exposed to control medium or specific pulses of luteinizing hormone (LH). In Expt 1, a frequency of 1 pulse LH/h (amplitude 500 pg/ml, duration 40 min, 30 ng/min) increased progesterone secretion compared with control values (P less than 0.05). In Expt 2, LH rate was held constant and the amplitude and duration of a single LH pulse varied; 250 and 500 ng LH/ml initially stimulated progesterone secretion equivalently, but increasing the duration of the LH pulse prolonged high progesterone secretion. These observations suggest that at less than or equal to 500 ng LH/ml, once a stimulatory amplitude is obtained, higher amplitudes do not further increase progesterone secretion, while increasing pulse duration further enhances progesterone secretion. In Expt 3, the LH pulse amplitude was 250 ng/ml and the rate set at 0, 5 or 30 ng LH/min; only 30 ng LH/min resulted in sustained stimulation of progesterone (P less than 0.05). Taken together, these data demonstrate that the characteristics which determine whether an LH pulse will be stimulatory include not only amplitude and duration but also the rate at which an amplitude is obtained.
authors.affiliation: Department of Obstetrics and Gynecology, University of Connecticut Health Center, Farmington 06032.
authors.forename: J C
authors.initials: JC
authors.lastname: Nulsen
authors.forename: S
authors.initials: S
authors.lastname: Kavel
authors.forename: J J
authors.initials: JJ
authors.lastname: Peluso
mesh_terms: D000818:Animals; D003338:Corpus Luteum / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D007986:Luteinizing Hormone / Q000494:pharmacology*; D010477:Perfusion; D011374:Progesterone / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical; D013997:Time Factors
chemical_list: D011374:Progesterone; D007986:Luteinizing Hormone