DNA of some anaerobic rumen fungi: G + C content determination.
The nuclear DNAs from five species of anaerobic rumen fungi have been isolated and purified by means of two extraction methods (with and without 8 M urea). Their G + C contents have been characterized by the thermal denaturation procedure of Marmur and Doty. As has already been shown in Neocallimastix frontalis, the results obtained by the two techniques demonstrated a very low G + C content (less than 20%) and the constant presence of satellite DNA.
Billon-Grand
G
G
ICBMC, Section Levures, Université Lyon 1, Villeurbanne, France.
Fiol
J B
JB
Breton
A
A
Bruyère
A
A
Oulhaj
Z
Z
D000818:Animals; D001482:Base Composition; D002417:Cattle; D004271:DNA, Fungal / Q000032:analysis*; D005658:Fungi / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D012417:Rumen / Q000382:microbiology*; D012418:Ruminants / Q000382:microbiology*
D004271:DNA, Fungal