Augmentation of cytotoxicity of tumor-infiltrating lymphocytes by biological response modifiers.
Lymphocyte infiltration into a tumor has been regarded as an expression of host immunity against cancer, but tumor-infiltrating lymphocytes (TIL) have little or no cytotoxicity. This study examined two different approaches to augment this low cytotoxicity. Firstly, biological response modifiers (OK-432, PSK) were injected into gastric cancer intralesionally. Intralesional injection of OK-432 or PSK significantly augmented the cytotoxicity of TIL. By the injection of OK-432, the ratio of OKT8-, Leu7-positive cells were increased in the TIL subset. In the second approach, TIL of gastric or pulmonary cancer patients were cultured with interleukin-2 (IL-2) in vitro. Co-culturing with IL-2 augmented the low cytotoxicity of TIL, and broad-reactive lymphokine-activated killer (LAK) cells were generated from TIL.
Gotoh
K
K
Department of Surgery, Iwakuni National Hospital, Japan.
Gouchi
A
A
Akura
Y
Y
Tanaka
N
N
Orita
K
K
D003602:Cytotoxicity, Immunologic; D006801:Humans; D007155:Immunologic Factors / Q000276:immunology / Q000494:pharmacology*; D015552:Injections, Intralesional; D007376:Interleukin-2 / Q000494:pharmacology; D008175:Lung Neoplasms / Q000276:immunology*; D016131:Lymphocyte Subsets; D016246:Lymphocytes, Tumor-Infiltrating / Q000187:drug effects / Q000276:immunology*; D010844:Picibanil / Q000494:pharmacology; D011509:Proteoglycans / Q000494:pharmacology; D013274:Stomach Neoplasms / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D007155:Immunologic Factors; D007376:Interleukin-2; D011509:Proteoglycans; D010844:Picibanil; C010770:polysaccharide-K