title: Characterization of proteins of chicken infectious anemia virus with monoclonal antibodies.
abstract: Eight monoclonal antibodies (MAbs) against chicken infectious anemia virus (CIAV) were developed. These MAbs identified three isolates adapted to grow in the Marek's disease chicken cell line MSB1 (Cux-1, GA-1, and Conn-B) and the chicken-propagated CIA-1 isolate. All MAbs stained MSB1 in the same way with mostly perinuclear staining, although larger nuclear inclusions and cytoplasmic staining were also detected. None of the MAbs neutralized Cux-1. All MAbs reacted in a direct enzyme-linked immunosorbent assay with Cux-1 antigen treated with 0.5% sodium dodecyl sulfate followed by extraction with chloroform, but not with MSB1 cells infected with Cux-1 or chloroform-extracts of these cells. Three viral proteins--VP1, VP2, and VP3--with estimated sizes of 45, 30, and 16 kilodaltons (kd), respectively, were immunoprecipitated using the MAbs and Cux-1-infected cell lysates. The 16-kd protein was the major VP. In addition, a 79-kd protein was detected in infected cell lysates by immunoprecipitation with CIAV-antibody-positive and -negative chicken serum, and CIAV-specific and non-specific MAbs.
authors.affiliation: Department of Avian and Aquatic Animal Medicine, College of Veterinary Medicine, Cornell University, Ithaca, New York 14850.
authors.forename: D
authors.initials: D
authors.lastname: Chandratilleke
authors.forename: P
authors.initials: P
authors.lastname: O'Connell
authors.forename: K A
authors.initials: KA
authors.lastname: Schat
mesh_terms: D000740:Anemia / Q000382:microbiology / Q000662:veterinary; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000918:Antibody Specificity; D002460:Cell Line; D002645:Chickens; D004267:DNA Viruses / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D051379:Mice; D011201:Poultry Diseases / Q000382:microbiology; D015531:Radioimmunoprecipitation Assay; D014764:Viral Proteins / Q000032:analysis*; D014777:Virus Diseases / Q000382:microbiology / Q000662:veterinary
chemical_list: D000911:Antibodies, Monoclonal; D014764:Viral Proteins