Structural analysis of the decanucleotide duplex d(GGTAATTACC)2 using 1H NMR spectroscopy.
The conformation of the decanucleotide duplex d(GGTAATTACC)2 has been investigated in solution by one- and two-dimensional proton NMR spectroscopy. Intra- and inter-nucleotide two-dimensional nuclear Overhauser enhancement data, recorded at mixing times between 15 and 250 ms, reveal a right-handed B-DNA structure. The data also show that the A-T basepairs of the TAATTA tract are highly propeller twisted and the minor groove is particularly narrow.
Embrey
K J
KJ
Victorian College of Pharmacy, School of Pharmaceutical Chemistry, Parkville, Vic., Australia.
Searle
M S
MS
Craik
D J
DJ
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry*
D009841:Oligonucleotides; D004247:DNA