Application of simultaneous determination of 3H, 14C, and 22Na by liquid scintillation counting to the measurement of cellular ion-transport.
A liquid scintillation counting method for simultaneous determination of three radioactive nuclides (3H, 14C, and 22Na) of biological interest was studied. By comparing the beta spectra of the three nuclides, their counting energy ranges, A, B, and C, were determined. 22NA was set high enough to avoid any spillover counts from lower-energy nuclides. Region A for 3H was set to maximize the counting efficiency. A good correlation between the counting efficiency for 22Na in region C and the counting efficiency of other nuclides in all regions was obtained. Prior to 3H and 14C dpm calculations, the 22Na counts spilled down in regions A and B were subtracted from the total counts in regions A and B. A simple linear equation was then used to compute 3H and 14C dpm. Findings show that the method presented is adaptable for highly quenched samples up to quenching indices of tSIE = 100. The method is useful for studying the biological transport coupled to Na+.
Miyazawa
Y
Y
Department of Radiochemistry--Biophysics, Niigata College of Pharmacy, Japan.
Sakai
N
N
Murakami
N
N
Konishi
T
T
D001610:Beta Particles; D001692:Biological Transport / Q000502:physiology / Q000528:radiation effects; D002250:Carbon Radioisotopes / Q000493:pharmacokinetics; D002412:Cations / Q000493:pharmacokinetics*; D019617:Halobacterium salinarum / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008027:Light; D011868:Radioisotopes / Q000493:pharmacokinetics*; D012588:Scintillation Counting / Q000379:methods*; D012979:Sodium Radioisotopes / Q000493:pharmacokinetics; D014316:Tritium / Q000493:pharmacokinetics
D002250:Carbon Radioisotopes; D002412:Cations; D011868:Radioisotopes; D012979:Sodium Radioisotopes; D014316:Tritium