A membrane blotting method for following the time course of protein radioiodination using Iodobeads.
A method for monitoring and optimizing the incorporation of radioiodide into protein is described. The use of immobilized chloramine-T (Iodobeads) combined with timed blots on nitrocellulose forms the basis of an efficient assay system. Results with Iodobeads and with the commonly utilized soluble form of chloramine-T are compared.
Cheng
H
H
Biology Department, Texas Woman's University, Denton 76204-1971.
Rudick
M J
MJ
D000042:Absorption; D002700:Chloramines; D003101:Collodion; D013006:Growth Hormone / Q000737:chemistry; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D011137:Polystyrenes; D011506:Proteins / Q000737:chemistry*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry; D014105:Tosyl Compounds
D002700:Chloramines; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D011137:Polystyrenes; D011506:Proteins; D014105:Tosyl Compounds; C016300:chloramine-T; D013006:Growth Hormone; D003101:Collodion; D012259:Ribonuclease, Pancreatic