Immune response of mice infected with recombinant vaccinia viruses carrying the HIV gag gene.
We examined mouse immune response to 4 kinds of recombinant vaccinia viruses carrying the HIV gag gene, including vac-gag/pol, which produces HIV-like particles with processed gag proteins; vac-gag, which also produces HIV-like particles but with unprocessed gag protein; and vac-gag-pol-fuse and vac-es-gag/pol, neither of which produces such particles but releases reverse transcriptase and gag protein, respectively, from infected cells. Although infection of mice with recombinant vaccinia viruses induced production of the anti-p24 antibody in all mice, vac-gag/pol and vac-es-pol induced higher production than the other two recombinants. Increase in [3H]thymidine uptake by splenic lymphocytes following p24 antigen stimulation was most evident in mice infected with vac-gag/pol. Thus, the highest immune reaction, both humoral and cellular, was elicited by vac-gag/pol, indicating that among those tested, this recombinant vaccinia virus is the best candidate for a vaccine that induces anti-HIV gag immunity.
Sugata
F
F
Department of Infectious Diseases, University of Tokyo, Japan.
Aoki
N
N
Shioda
T
T
Hayashi
T
T
Shimada
K
K
Mitamura
K
K
Shibuta
H
H
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D015152:Blotting, Northern; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015750:Genes, gag / Q000276:immunology*; D015751:Genes, pol / Q000235:genetics; D015483:HIV Antibodies / Q000276:immunology; D016655:HIV Core Protein p24 / Q000276:immunology; D015497:HIV-1 / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D013045:Species Specificity; D014162:Transfection; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology*; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D015483:HIV Antibodies; D016655:HIV Core Protein p24; D014614:Vaccines, Synthetic