Evaluation of antigen-antibody affinity constants by partition equilibrium studies with a two-phase aqueous polymer system: a more rigorous analysis.
A quantitative procedure is devised for the determination of affinity constants from the composition-dependence of the antigen partition coefficient in liquid-liquid partition studies of antigen-antibody mixtures. Its use is illustrated by application to published results (Elling, L.R., Kula, M.-R., Hadas, E. and Katchalski-Katzir, E. (1991) Anal. Biochem. 192, 74-77) obtained for the interaction of horseradish peroxidase with an elicited monoclonal antibody by antigen distribution studies with a two-phase aqueous polymer system (poly(ethylene glycol)/dextran) for the partition analysis. Shortcomings in the original interpretation of these results are eliminated.
Winzor
D J
DJ
Department of Biochemistry, University of Queensland, Brisbane, Australia.
D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D006735:Horseradish Peroxidase; D008433:Mathematics; D011108:Polymers
D000911:Antibodies, Monoclonal; D011108:Polymers; D006735:Horseradish Peroxidase