[Changes in glycine-activated conductivity of membranes of chick embryo neurons in culture].
Glycine-activated whole-cell currents of the chick spinal cord neurons have been investigated in a chicken embryo during different periods in culture. Shifts in the concentration dependence curve of mature and immature cells are shown. Mature cells prove to be motoneurons and die 5-7 days later in vitro. immature cells are neuroblasts differentiating in culture. Glycine sensitivity of low-differentiating cells increased more than 10 times by the end of the 14th day in culture. Desensitization kinetics of the glycine-activated currents decelerated with age. A crucial role of spontaneous electric activity was shown for maturation of the agonist-activated conductance.
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D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology; D002642:Chick Embryo; D004553:Electric Conductivity / Q000502:physiology; D005998:Glycine / Q000494:pharmacology*; D007700:Kinetics; D009046:Motor Neurons / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects*
D005998:Glycine
Izmeneniia glitsinaktiviruemoĭ provodimosti membran neĭronov spinnogo mozga kurinogo émbriona v protsesse kul'tivirovaniia.