Diversity of Mobiluncus strains as demonstrated by their electrophoretic protein patterns.
The analysis of electrophoretic protein profiles of 21 Mobiluncus curtisii, 11 Mobiluncus mulieris and 3 reference strains (Mobiluncus curtisii subsp. curtisii ATCC 35241, Mobiluncus curtisii subsp. holmesii ATCC 35242 and Mobiluncus mulieris BV 64-5) demonstrated species-related patterns. A highly variable region appeared at 70-85 kDa for Mobiluncus curtisii and at 75-95 kDa for Mobiluncus mulieris, which was likely to correspond to cell surface located proteins. When performed under standardized conditions, PAGE-protein analysis allowed to define intraspecies clusters, from which some strains appeared identical. Thus, the method seemed to provide a useful additive to identify a strain at the species level and might be of epidemiological interest.
Drouet
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EB
Department of Microbiology-Virology, Institut Pasteur de Lyon, France.
Boude
M
M
Denoyel
G A
GA
D001421:Bacteria, Anaerobic / Q000737:chemistry* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D015203:Reproducibility of Results; D014566:Urogenital System / Q000382:microbiology
D001426:Bacterial Proteins