A comparative study of the effects of clofibrate, ciprofibrate, WY-14,643, and di-(2-ethylhexyl)-phthalate on liver protein expression in mice.
Chemical, physical, or mechanical insults to a cellular system cause changes in the rates of protein synthesis or degradation. To define patterns of quantitative protein changes and identify the biochemical basis of those changes, we are studying fluctuations in mouse liver protein abundance in response to factors such as stages of maturation, changes in diet, and exposure to chemicals. Two-dimensional gel electrophoresis (2DE) together with computer-assisted data analysis is used to collect quantitative data from groups of 2DE patterns. Our analysis of liver protein expression in mice treated with four different chemicals known to cause peroxisome proliferation serves as an example of the changes in protein expression that can be monitored by using this approach. The database we are building indicates that changes specific to a particular treatment as well as generalized effects can be identified, providing the opportunity to identify patterns of protein expression that are indicators of biological response to different types of cellular insults.
Giometti
C S
CS
Biological and Medical Research Division, Argonne National Laboratory, Illinois 60439.
Taylor
J
J
Gemmell
M A
MA
Tollaksen
S L
SL
Lalwani
N D
ND
Reddy
J K
JK
D000818:Animals; D002995:Clofibric Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D004051:Diethylhexyl Phthalate / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D058607:Fibric Acids; D000960:Hypolipidemic Agents / Q000494:pharmacology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis / Q000187:drug effects*; D051379:Mice; D008830:Microbodies / Q000187:drug effects; D011743:Pyrimidines / Q000494:pharmacology*
D058607:Fibric Acids; D000960:Hypolipidemic Agents; D008565:Membrane Proteins; D011743:Pyrimidines; D002995:Clofibric Acid; C006253:pirinixic acid; D004051:Diethylhexyl Phthalate; C019304:ciprofibrate