Determination of free salsolinol concentrations in human urine using gas chromatography-mass spectrometry.
The urine concentrations of free salsolinol were determined in six healthy volunteers, using a gas chromatographic-mass spectrometric method with electron-capture negative-ion chemical ionization after derivatization with pentafluoropropionyl anhydride. The sensitivity of this method allows the quantification of salsolinol concentrations of 0.55 pmol/ml. The synthesis of [2H4]salsolinol from dopamine and [2H4]acetaldehyde via a Pictet-Spengler condensation is described; [2H4]salsolinol was used as the internal standard for salsolinol quantification. The urine concentrations of free salsolinol ranged from ca. 1 to 6 pmol/ml.
Allievi
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Farmitalia Carlo Erba, Research and Development, Erbamont Group, Milan, Italy.
Dostert
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Strolin Benedetti
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D004583:Electrons; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007477:Ions; D007546:Isoquinolines / Q000652:urine*; D012016:Reference Values; D015203:Reproducibility of Results
D007477:Ions; D007546:Isoquinolines; C036617:salsolinol