title: Amperometric alcohol electrode with extended linearity and reduced interferences.
abstract: Conventional amperometric alcohol electrodes were constructed with oxygen- and hydrogen peroxide-base sensors and a much improved electrode was designed by placing a hydrophobic, gas-permeable membrane over the conventional hydrogen peroxide-based alcohol electrode. The immobilization of alcohol oxidase with glutaraldehyde was also studied and optimized. The upper linear ranges of the conventional and newly designed alcohol electrodes were 0.02 and 0.5% ethanol, respectively. The hydrophobic membrane of the new design eliminated the classical electrochemical interferences of hydrogen peroxide-based electrodes and the typical pH dependence of enzymatic systems.
authors.affiliation: Universal Sensors, Incorporated, Metairie, Louisiana 70006.
authors.forename: G J
authors.initials: GJ
authors.lastname: Lubrano
authors.forename: M H
authors.initials: MH
authors.lastname: Faridnia
authors.forename: G
authors.initials: G
authors.lastname: Palleschi
authors.forename: G G
authors.initials: GG
authors.lastname: Gyilbault
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized; D000431:Ethanol / Q000032:analysis*; D005976:Glutaral / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis*; D008567:Membranes, Artificial; D000432:Methanol / Q000032:analysis*; D011076:Polycarboxylate Cement / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D011076:Polycarboxylate Cement; C005506:polycarbonate; D000431:Ethanol; D006861:Hydrogen Peroxide; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase; D005976:Glutaral; D000432:Methanol