title: The distribution of actin immunoreactivity in rhabdomeres of tipulid flies in relation to extracellular membrane shedding.
abstract: Rhabdomeres of tipulid flies lose membrane during turnover from a 'shedding zone' composed of microvillar tips. These distal domains lack intramicrovillar cytoskeletons and appear to be empty sacs of membrane. Recent concerns about the role of ninaC mechano-enzymes in the architecture of dipteran rhabdomeral microvilli and the dynamic role that they may play in the creation of shedding zones demand an examination of the distribution of actin in tipulid rhabdomeres. We compared rhabdomeres from tipulid retinae incubated before fixation for immunocytochemistry in a buffer without additives and a stabilising buffer that contained a cocktail of cysteine protease inhibitors; both were challenged by an anti-actin antibody for immunogold labelling after embedding in LR White Resin. Shedding zones thus processed collapse to structureless detritus. Stabilised and unstabilized shedding zones were immunonegative to anti-actin. To ensure that the negative results were not consequent upon conformational changes generated by the processing protocol, we examined microvilli of degenerating rhabdomeres of the Drosophila light-dependent retinal degeneration mutant rdgBKS222 (which separate and collapse without creating a shedding zone) and found the detritus they generate to be immunopositive to anti-actin. Stabilised and unstabilized regions of basal regions of tipulid rhabdomeres were equally immunopositive. We infer that (a) actin is absent from shedding zones; (b) actin is not degraded by microvillar cysteine proteases. The implications of these conclusions are discussed in relation to some functional models of arthropod photoreceptor microvilli.
authors.affiliation: Developmental Neurobiology Group, Research School of Biological Sciences, Australian National University, Canberra.
authors.forename: A D
authors.initials: AD
authors.lastname: Blest
authors.forename: S
authors.initials: S
authors.lastname: Stowe
authors.forename: J A
authors.initials: JA
authors.lastname: Clausen
authors.forename: M
authors.initials: M
authors.lastname: Carter
mesh_terms: D000199:Actins / Q000032:analysis* / Q000276:immunology; D000818:Animals; D002645:Chickens; D003429:Cross Reactions; D003599:Cytoskeleton / Q000648:ultrastructure*; D004175:Diptera / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure*; D007150:Immunohistochemistry; D008871:Microvilli / Q000648:ultrastructure; D008970:Molecular Weight; D011817:Rabbits; D012160:Retina / Q000502:physiology / Q000648:ultrastructure*
chemical_list: D000199:Actins