[A study of DEAE ion exchange chromatography for the purification of hen's yolk IgG].
A DEAE ion exchange chromatography procedure performed by using either gradient or stepwise elution was developed for the purification of chicken IgG antibodies from the egg yolk of immunized hens. Following this procedure, highly purified yolk IgG preparations were obtained as identified by Sephadex G 200 gel filtration as well as sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Moreover, this procedure guaranteed the recovery of antibodies of undiminished activity. The results demonstrate that the procedure is simple, reliable and practical for the yolk IgG purification. The establishment of such a method is beneficial to the production and application of egg yolk antibodies.
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D000818:Animals; D002645:Chickens; D002852:Chromatography, Ion Exchange; D004530:Egg Yolk / Q000737:chemistry*; D007074:Immunoglobulin G / Q000302:isolation & purification*
D007074:Immunoglobulin G