Automated determination of orotic acid, uracil and pseudouridine in urine by high-performance liquid chromatography with column switching.
A column-switching high-performance liquid chromatographic method, requiring no sample preparation apart from filtration, is described for quantification of urinary orotic acid, uracil and pseudouridine. The analyses were carried out using a reversed-phase octadecylsilane-bonded column for sample clean-up and a cation-exchange column for separation; 5-20 microliters samples of urine were directly analysed, and more than 100 samples could be analysed consecutively. Each sample required only 30 min. Detection limits of these compounds were 5 pmol. Creatinine-related urinary uracil excretion was lowest in the newborn period (17.3 +/- 14.4 mumol/g of creatinine). A patient with partial ornithine transcarbamylase deficiency and his mother usually excreted a high level of uracil during the period of normal orotic acid excretion and normal serum ammonia level.
Ohba
S
S
Department of Pediatrics, Nagoya City Higashi General Hospital, Japan.
Kidouchi
K
K
Katoh
T
T
Kibe
T
T
Kobayashi
M
M
Wada
Y
Y
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007223:Infant; D008661:Metabolism, Inborn Errors / Q000652:urine; D020163:Ornithine Carbamoyltransferase Deficiency Disease; D009963:Orotic Acid / Q000652:urine*; D011560:Pseudouridine / Q000652:urine*; D013056:Spectrophotometry, Ultraviolet; D014498:Uracil / Q000652:urine*
D011560:Pseudouridine; D014498:Uracil; D009963:Orotic Acid