title: Ascorbic acid derivatives in two different fractions of uremic toxins.
abstract: The middle-molecular-weight uremic toxins which accumulate in uremic plasma seem to be associated with various uremic disorders such as uremic neuropathy and defects in the sodium pump. By a multi-step chromatographic method, two fractions of these toxins were isolated and studied because one inhibits microtubule formation in vitro (fraction 2-5), and the other impairs the sodium pump in living erythrocytes (fraction 2-3). An additional chromatographic method allows the separation of these fractions and isolation of two components: fractions 2-3-V and 2-5-III. Analyses by UV and 1H NMR spectrometry identified these compounds as two different ascorbic acid derivatives. 2-3-V is not yet totally identified and 2-5-III corresponds to ascorbic acid 2-sulfate. These two metabolites exert no toxic effects but they have the same chromatographic behavior as uremic toxins.
authors.affiliation: Biophysics Laboratory, UFR de Pharmacie, Marseille, France.
authors.forename: P M
authors.initials: PM
authors.lastname: Gallice
authors.forename: J P
authors.initials: JP
authors.lastname: Monti
authors.forename: D L
authors.initials: DL
authors.lastname: Braguer
authors.forename: M
authors.initials: M
authors.lastname: Baz
authors.forename: R E
authors.initials: RE
authors.lastname: Elsen
authors.forename: Y F
authors.initials: YF
authors.lastname: Berland
authors.forename: A D
authors.initials: AD
authors.lastname: Crevat
mesh_terms: D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet; D014118:Toxins, Biological / Q000737:chemistry*; D014511:Uremia / Q000097:blood*
chemical_list: D014118:Toxins, Biological; C026865:uremia middle molecule toxins; D001205:Ascorbic Acid