Inhibition of protein tyrosine phosphatase activity by diamide is reversed by epidermal growth factor in fibroblasts.
Diamide (azodicarboxylic acid bis(dimethylamide] inhibits protein tyrosine phosphatase activity in fibroblasts without altering protein tyrosine kinase activity associated with the epidermal growth factor receptor. The loss of protein tyrosine phosphatase activity caused by diamide is reversed by 2-mercaptoethanol or epidermal growth factor.
Monteiro
H P
HP
Department of Pharmacology, New York University Medical Center, NY 10016.
Ivaschenko
Y
Y
Fischer
R
R
Stern
A
A
D016475:3T3 Cells; D000818:Animals; D003958:Diamide / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D066246:ErbB Receptors / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D008623:Mercaptoethanol / Q000494:pharmacology; D051379:Mice; D010766:Phosphorylation; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014162:Transfection
D003958:Diamide; D008623:Mercaptoethanol; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D017027:Protein Tyrosine Phosphatases