Proteins and glycosaminoglycans in the intercellular matrix of the human cumulus-oophorus and their effect on conversion of proacrosin to acrosin.
Human cumuli-oophori were cultured in vitro in the presence of radioactive protein and polysaccharide precursors. The time course of the cumulus cell secretion was traced by histoautoradiography. Matrix solubilization, and sodium dodecyl sulphate polyacrylamide gel electrophoresis and high-performance liquid chromatography showed that proteoglycan (Mr greater than 1,700,000) was the main cumulus cell product that was prevailingly deposited in the cumulus intercellular matrix and partly released into the culture medium. It was capable of accelerating the conversion of proacrosin to acrosin and this activity was abolished by enzymatic removal of chondroitin sulphate, the predominant glycosaminoglycan of this proteoglycan fraction. None of the other fractions, including a proteoglycan of Mr 80,000-90,000, containing heparan sulphate, accelerated the conversion of proacrosin to acrosin under the conditions used. The results suggest that chondroitin sulphate is the active component of the high-Mr proacrosin activator of the human cumulus-oophorus.
Drahorád
J
J
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
Tesarík
J
J
Cechová
D
D
Vilím
V
V
D000176:Acrosin / Q000096:biosynthesis* / Q000378:metabolism*; D001345:Autoradiography; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004789:Enzyme Activation / Q000502:physiology; D004792:Enzyme Precursors / Q000378:metabolism*; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006801:Humans; D008855:Microscopy, Electron, Scanning; D006080:Ovarian Follicle / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D011509:Proteoglycans / Q000032:analysis / Q000096:biosynthesis*
D004792:Enzyme Precursors; D011509:Proteoglycans; D002809:Chondroitin Sulfates; C022438:proacrosin; D000176:Acrosin