Stimulation of NADH oxidation by xanthine oxidase and polyvanadate in presence of some dehydrogenases and flavin compounds.
The rates of NADH oxidation in presence of xanthine oxidase increase to a small and variable extent on addition of high concentrations of lactate dehydrogenase and other dehydrogenases. This heat stable activity is similar to polyvanadate-stimulation with respect to pH profile and SOD sensitivity. Isocitric dehydrogenase (NADP-specific) showed heat labile, SOD-sensitive polyvanadate-stimulated NADH oxidation activity. Polyvanadate-stimulated SOD-sensitive NADH oxidation was also found to occur with riboflavin, FMN and FAD in presence of a non-specific protein, BSA, suggesting that some flavoproteins may possess this activity.
Penta
K
K
Department of Biochemistry, Indian Institute of Science, Bangalore.
Gullapalli
S
S
Rau
M
M
Ramasarma
T
T
D005486:Flavin Mononucleotide / Q000494:pharmacology; D005182:Flavin-Adenine Dinucleotide / Q000494:pharmacology; D005415:Flavins / Q000494:pharmacology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007521:Isocitrate Dehydrogenase / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000494:pharmacology; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000494:pharmacology*; D011489:Protein Denaturation; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology; D014638:Vanadates / Q000494:pharmacology*; D014969:Xanthine Oxidase / Q000494:pharmacology*
D005415:Flavins; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D012710:Serum Albumin, Bovine; D014638:Vanadates; D005486:Flavin Mononucleotide; D010088:Oxidoreductases; D007770:L-Lactate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase