title: Induction of multipolar mitoses in cultured cells: decay and restructuring of the mitotic apparatus and distribution of centrioles.
abstract: During recovery after a long (up to 12 h) treatment of pig embryo culture cells (PK) with nocodazole at concentrations of 0.02 microgram/ml and 0.2 microgram/ml all c-metaphase cells divide normally into two daughter cells. During recovery after a short (1-4 h) treatment with 0.6 microgram/ml nocodazole only multipolar mitoses (as a rule tripolar) arise. At the ultrastructural level, the increasing nocodazole concentration leads to progressive disruption of the mitotic spindle. At a nocodazole concentration of 0.2 microgram/ml kinetochores are not associated with microtubules. At a nocodazole concentration of 0.6 microgram/ml there are no microtubules around the centrosomes, and in every cell one of the two diplosomes disintegrates. In tripolar telophase centrioles are distributed among the spindle poles generally in a 2:2:0 pattern. Mother and daughter centrioles are always disoriented but not separated. The centriole-free pole contains a cloud of electron-dense material. During tripolar division two of the three daughter cells mainly fuse shortly after telophase forming one binucleate cell. Thus a multipolar mitosis arises as a result of the uncoupling of mother centrioles and spindle microtubules, but not of the duration of the c-mitotic arrest. Centriole-free poles account for the divergence of chromosomes, but mainly they are unable to ensure the normal cytokinesis of daughter cells.
authors.affiliation: A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
authors.forename: I B
authors.initials: IB
authors.lastname: Alieva
authors.forename: I A
authors.initials: IA
authors.lastname: Vorobjev
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002502:Centrioles / Q000187:drug effects* / Q000648:ultrastructure; D007700:Kinetics; D008677:Metaphase; D008854:Microscopy, Electron; D008938:Mitosis / Q000187:drug effects*; D015739:Nocodazole / Q000494:pharmacology*; D008941:Spindle Apparatus / Q000187:drug effects* / Q000648:ultrastructure; D013552:Swine
chemical_list: D015739:Nocodazole