Fluctuation of histone H1 kinase activity during meiotic maturation in porcine oocytes.
Porcine oocytes cultured in follicular fluid for various periods of up to 48 h were stained with Hoechst-33342 and classified according to maturation before assaying. Histone H1 kinase activity at metaphase I was approximately 10 times that at the germinal vesicle stage. An abrupt reduction in activity was observed in oocytes emitting the first polar body; then the activity increased again to the same level as at metaphase I. This pattern is similar to those reported in non-mammalian species and supports the concepts that histone H1 kinase is ubiquitous in eukaryotes and controls the meiotic cell cycle in mammals.
Naito
K
K
Institute of Medical Science, University of Tokyo, Japan.
Toyoda
Y
Y
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D016200:Maturation-Promoting Factor / Q000378:metabolism*; D008540:Meiosis / Q000502:physiology*; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000166:cytology / Q000201:enzymology*; D010766:Phosphorylation; D013552:Swine / Q000378:metabolism*
D016200:Maturation-Promoting Factor