[Cloning of the gene and immunochemical specificity of recombinant immunoglobulin binding protein V7 from Streptococcus valente (group G)].
The fragment of the structural gene coding for the Fc-receptor of Streptococcus Valente (G group) has been cloned. The resulting recombinant plasmid pPGSV1 contains the O, kb HindIII fragment of streptococcal chromosomal DNA inserted into the vector plasmid pUC19 and determines the expression of the 31 kD protein in Escherichia coli cells. The protein binds the immunoglobulins of human, rabbit, guinea pig origin, but in contrast to the G protein of another G group streptococcus it is nonreactive with mouse, pig and sheep IgG.
Spirina
G V
GV
Smirnov
O Iu
OIu
Chernovskaia
T V
TV
Abramov
V M
VM
D001426:Bacterial Proteins / Q000235:genetics*; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007150:Immunohistochemistry; D010957:Plasmids; D011961:Receptors, Fc / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics; D013045:Species Specificity; D013291:Streptococcus / Q000276:immunology / Q000378:metabolism*; D013379:Substrate Specificity
D001426:Bacterial Proteins; D007074:Immunoglobulin G; D011961:Receptors, Fc; D011994:Recombinant Proteins; C072831:V7 protein, Streptococcus sp. 'group G'
Klonirovanie gena i immunokhimicheskaia spetsifichnost' rekombinantnogoimmunoglobulinsviazyvaiushchego belka V7 Streptococcus valenta (gruppa G).