Recent advances in liquid chromatography-mass spectrometry and capillary zone electrophoresis-mass spectrometry for protein analysis.
The utility of the combination of separations techniques, such as liquid chromatography and capillary zone electrophoresis, with mass spectrometry in applications involving protein analysis is discussed. The use of continuous-flow fast atom bombardment and electrospray ionization mass spectrometry is compared for the analysis of tryptic digests. For liquid chromatography, both microbore and slurry-packed capillary bore columns were used to separate peptides from proteolytic digests.
Suter
M J
MJ
Analytical Chemistry Center, University of Texas Medical School, Houston 77225.
DaGue
B B
BB
Moore
W T
WT
Lin
S N
SN
Caprioli
R M
RM
D002853:Chromatography, Liquid / Q000379:methods*; D004586:Electrophoresis / Q000379:methods*; D013006:Growth Hormone / Q000032:analysis; D006801:Humans; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014357:Trypsin
D010455:Peptides; D011506:Proteins; D013006:Growth Hormone; D014357:Trypsin