Isolation of a human chromosome 14-only somatic cell hybrid: analysis using Alu and LINE-based PCR.
Interspecific somatic cell hybrids containing single human chromosomes are valuable reagents for localization of cloned genes and DNA fragments to specific chromosomes, for the development of chromosome-specific libraries, and for generation of hybrid cell lines containing subchromosomal regions. A CHO somatic cell hybrid containing a single, intact human chromosome 14 (MHR14) was developed and confirmed by LINE PCR amplification gel pattern, by Alu-517 PCR product dot blot hybridization, and by cytogenetic analysis. MHR14 will serve as the chromosome source for the development of a radiation map of human chromosome 14.
Mares
A
A
Department of Medicine, Baylor College of Medicine, Houston, Texas 77030.
Ledbetter
S A
SA
Ledbetter
D H
DH
Roberts
R
R
Hejtmancik
J F
JF
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002469:Cell Separation; D002871:Chromosome Banding; D002883:Chromosomes, Human, Pair 14; D006224:Cricetinae; D004247:DNA; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
D004247:DNA