Principles of stopped-flow sequential injection analysis and its application to the kinetic determination of traces of a proteolytic enzyme.
The concept of sequential injection analysis is described, and an application of this novel technique to an assay of traces of a proteolytic enzyme is demonstrated. The automated method is based on a stopped-flow procedure with fluorescence detection. Instead of using the conventional constant flow rate, a sinusoldal flow pattern, as generated by a robust cam-driven piston pump, has been applied. The validation of the novel flow injection system using a nonreactive fluorescent compound yielded highly reproducible results over a concentration range of 3 order of magnitude. The calibration graph for the enzyme activities in the range 5-75 micron mL-1 could be fitted a second-order polynomial. The detection limit is 2.5 micron mL-1, which corresponds to 7.2 ng mL-1 pure active enzyme.
Ruzicka
J
J
Department of Chemistry, University of Washington, Seattle 98195.
Gübeli
T
T
D010450:Endopeptidases / Q000032:analysis*; D017022:Flow Injection Analysis; D007700:Kinetics; D013050:Spectrometry, Fluorescence
D010450:Endopeptidases