title: Surface active substances in the chinchilla tubotympanum. A biochemical study.
abstract: It has been demonstrated that the eustachian tube and middle ear epithelium produce Tubal Surface Active Substances (TSAS), which facilitate the opening of the eustachian tube. In order to characterize the biochemical contents of chinchilla TSAS, the tubal washings were analyzed using 2-D thin layer chromatography. The results indicate that phosphatidylcholine was the predominant phospholipid, followed by sphingomyelin, phosphatidylinositol phosphatidylethanolamine, and phosphatidylserine. In comparison, pulmonary lavage showed phosphatidylcholine to be highest allowed by phosphatidylethanolamine and sphingomyelin. Phosphatidylcholine/phosphatidylethanolanim ratios were 5:1 in the tubal lavage, and 8:1 in the pulmonary lavage. Phosphatidylcholine/sphingomyelin ratios were 2:1 in the tubal lavage, and 67:1 in the pulmonary lavage. It is concluded that the biochemical content of TSAS is similar but not identical to that of pulmonary surfactants.
authors.affiliation: Otological Research Laboratories, Ohio State University College of Medicine, Columbus 43210.
authors.forename: J M
authors.initials: JM
authors.lastname: Coticchia
authors.forename: F A
authors.initials: FA
authors.lastname: Heiselman
authors.forename: R
authors.initials: R
authors.lastname: Gharbo
authors.forename: T F
authors.initials: TF
authors.lastname: DeMaria
authors.forename: D J
authors.initials: DJ
authors.lastname: Lim
mesh_terms: D000818:Animals; D002682:Chinchilla / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D004432:Ear, Middle / Q000737:chemistry*; D005064:Eustachian Tube / Q000737:chemistry*; D010743:Phospholipids / Q000032:analysis*; D011663:Pulmonary Surfactants / Q000032:analysis; D013501:Surface-Active Agents / Q000032:analysis; D007507:Therapeutic Irrigation; D014132:Trachea / Q000737:chemistry
chemical_list: D010743:Phospholipids; D011663:Pulmonary Surfactants; D013501:Surface-Active Agents