Familial combined hyperlipidaemia: use of stable isotopes to demonstrate overproduction of very low-density lipoprotein apolipoprotein B by the liver.
We have assessed very low-density lipoprotein apolipoprotein B production, using [15N]glycine as an endogenous marker in a 9-hour primed constant infusion protocol, in four adult male subjects with familial combined hyperlipidaemia and in four normolipidaemic adult male controls. The mean very low-density lipoprotein apolipoprotein B absolute synthetic rate was significantly greater in the familial combined hyperlipidaemic subjects than in control subjects (26.31 +/- 8.37 vs 9.36 +/- 4.07 mg/kg per 24 h, p less than 0.05). These results confirm findings using exogenous radioisotope labelling techniques that very low-density lipoprotein apolipoprotein B production is significantly increased in most patients with familial combined hyperlipidaemia. A modified 9-hour protocol can be safely done repeatedly and in children and pregnant women.
Cortner
J A
JA
Lipid-Heart Research Center, Children's Hospital of Philadelphia, PA 19104.
Coates
P M
PM
Bennett
M J
MJ
Cryer
D R
DR
Le
N A
NA
D000328:Adult; D001055:Apolipoproteins B / Q000096:biosynthesis*; D002784:Cholesterol / Q000097:blood; D005998:Glycine / Q000378:metabolism; D006801:Humans; D006950:Hyperlipidemia, Familial Combined / Q000378:metabolism*; D008079:Lipoproteins, VLDL / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism*; D008297:Male; D009587:Nitrogen Isotopes; D014280:Triglycerides / Q000097:blood
D001055:Apolipoproteins B; D008079:Lipoproteins, VLDL; D009587:Nitrogen Isotopes; D014280:Triglycerides; D002784:Cholesterol; D005998:Glycine