title: Binding studies and localization of Escherichia coli lipopolysaccharide in cultured hepatocytes by an immunocolloidal-gold technique.
abstract: In this study, the uptake and localization of an Escherichia coli lipopolysaccharide, and the temperature effects on these processes, were studied in rat cultured hepatocytes using a binding assay and an immunocolloidal gold technique. The lipopolysaccharide was found to bind to the cell membrane and microvilli after short incubation times, at both 4 degrees C and 37 degrees C. This was followed by a dispersed localization into the cytoplasm, reaching mitochondria. The uptake was found not to be receptor-mediated. A decrease of temperature, delays, but does not prevent, the lipopolysaccharide internalization.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Chemistry, Universidad Complutense, Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Díaz-Laviada
authors.forename: J
authors.initials: J
authors.lastname: Ainaga
authors.forename: M T
authors.initials: MT
authors.lastname: Portolés
authors.forename: J L
authors.initials: JL
authors.lastname: Carrascosa
authors.forename: A M
authors.initials: AM
authors.lastname: Muncio
authors.forename: R
authors.initials: R
authors.lastname: Pagani
mesh_terms: D000818:Animals; D001665:Binding Sites; D002478:Cells, Cultured; D004926:Escherichia coli / Q000737:chemistry*; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000032:analysis*; D008099:Liver / Q000382:microbiology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D008070:Lipopolysaccharides