[Preliminary studies of notoginsenoside R1-induced differentiation of HL-60 cell lines in vitro].
Our experiments have proved that human promyelocytic leukemia cells (HL-60) could be induced to differentiate in vitro by notoginsenoside R1 into mature neutrophils. The morphological results showed that 68% of HL-60 cells were differentiated in 80 microgram/ml inducer for 5 days, in which metamyelocytes were 32%, banded neutrophils 30% and segmented neutrophils 6% 3H-TdR and 3H-UR incorporate assay showed that notoginsenoside R1 effected synthesis of DNA and RNA while it induced differentiation of HL-60 cell. At 48 h, percent of incorporate inhibition was 26.32% with 3H-TdR and 18.57% with 3H-UR; at 72 h, 17.46% and 21.76%, respectively.
Xu
L
L
Department of Pathophysiology.
Wang
B
B
Gao
J
J
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D036145:Ginsenosides; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D012503:Saponins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D036145:Ginsenosides; D012503:Saponins; C072936:notoginsenoside R1