Effect of methyl esterification of isoglutamine residue in 6-O-acylated muramyl dipeptides on expression of immunopharmacological activities.
The effect of methyl esterification of the isoglutamine residue in 6-O-acylated muramyl dipeptide (MDP) on some biological activities was investigated. Methyl esterification influenced more or less the expression of all activities tested. The adjuvant and colony stimulating factor (CSF)-inducing activities of 6-O-acylated MDP analogs carrying a 3-hexadecanoyloxytetradecanoyl [C14-O-(C16)] group were stronger than those of the corresponding analogs carrying a 2-tetradecylhexadecanoyl (B30) group. Macrophage activation, i.e. induction of tumor necrosis factor (TNF) and promotion of phagocytosis, by 6-O-C14-O-(C16)-MDP methyl esters was weaker.
Furuya
T
T
School of Hygeinic Sciences, Kitasato University, Sagamihara, Japan.
Kumazawa
Y
Y
Takimoto
H
H
Nagumo
T
T
Kiso
M
M
Hasegawa
A
A
Nomoto
K
K
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D007274:Injections, Intraperitoneal; D008262:Macrophage Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology
D003115:Colony-Stimulating Factors; D006514:Hepatitis B Surface Antigens; D000119:Acetylmuramyl-Alanyl-Isoglutamine