Measurement of fibrinogen adsorption from blood plasma using 125I-fibrinogen and a direct ELISA technique.
A direct enzyme-linked immunosorbent assay (ELISA), using a polyclonal anti-fibrinogen conjugated to horseradish peroxidase, was used to detect fibrinogen adsorption from blood plasma to ten different materials. Adsorption was also measured with [125I]-fibrinogen. The materials studied included glass, Biomer, Immulon I, and a series of hydroxyethylmethacrylate (HEMA) and ethylmethacrylate (EMA) co-polymers. For all the materials studied, the results from the ELISA technique closely paralleled those obtained using [125I]-fibrinogen. The cross-reactivity of the antibody with proteins other than fibrinogen was generally small. Both experimental methods detected the presence of a maximum in fibrinogen adsorption (as a function of the plasma dilution) to the more hydrophobic materials. For all but two HEMA/EMA co-polymers, a linear correlation between the ELISA and [125I]-fibronogen measurements was indicated by inspection of cross plots as well as by a statistical test.
Slack
S M
SM
Department of Chemical Engineering, University of Washington, Seattle 98195.
Posso
S E
SE
Horbett
T A
TA
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005340:Fibrinogen / Q000378:metabolism*; D005898:Glass / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D010215:Papio; D010949:Plasma / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry
D001672:Biocompatible Materials; D008689:Methacrylates; D011108:Polymers; D011140:Polyurethanes; C031892:biomer; C005044:hydroxyethyl methacrylate; D005340:Fibrinogen