title: Exercise-induced phospholipid degradation in the equine skeletal muscle and erythrocytes.
abstract: To understand the pathogenesis of equine exercise-induced myopathies and hemolysis, changes of phospholipid peroxidation products in the equine middle gluteal muscle and erythrocytes following the high-speed treadmill exercise were studied. In the skeletal muscle, the peroxidized phosphatidylethanolamine (PE) were increased at 24 hours after the exercise. The malondialdehydes (MDAs) were also increased as the protein-bound MDAs following exercise. In the erythrocytes, the peroxidized PE were significantly decreased at 24 hours after the exercise. The protein-bound MDAs were significantly increased at 5 min after the exercise and returned to the base values at 24 hours after the exercise. These findings indicate that the PE is more susceptible to in vivo oxidative effects than the other phospholipid classes, and the accumulation of the protein-bound MDAs is considered to play some cytotoxic roles in the equine skeletal muscle and erythrocytes following exercise.
authors.affiliation: Department of Veterinary Clinical Pathobiology, Faculty of Agriculture, University of Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Matsuki
authors.forename: S
authors.initials: S
authors.lastname: Tamura
authors.forename: K
authors.initials: K
authors.lastname: Ono
authors.forename: T
authors.initials: T
authors.lastname: Watari
authors.forename: R
authors.initials: R
authors.lastname: Goitsuka
authors.forename: A
authors.initials: A
authors.lastname: Yamanobe
authors.forename: A
authors.initials: A
authors.lastname: Hiraga
authors.forename: K
authors.initials: K
authors.lastname: Kubo
authors.forename: S
authors.initials: S
authors.lastname: Takagi
authors.forename: A
authors.initials: A
authors.lastname: Hasegawa
mesh_terms: D000818:Animals; D003402:Creatine Kinase / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006400:Hematocrit / Q000662:veterinary; D006736:Horses / Q000097:blood / Q000502:physiology*; D007773:Lactates / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D009132:Muscles / Q000378:metabolism*; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000097:blood / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000097:blood / Q000378:metabolism; D010716:Phosphatidylinositols / Q000097:blood; D010718:Phosphatidylserines / Q000097:blood; D010743:Phospholipids / Q000097:blood / Q000378:metabolism*; D005082:Physical Exertion / Q000502:physiology*
chemical_list: D007773:Lactates; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D010718:Phosphatidylserines; D010743:Phospholipids; D008315:Malondialdehyde; D003402:Creatine Kinase