[Determination of bacterial N portions in feces and differently collected ileum chymus from swine].
In faeces and ileal digesta samples of 31 intact (INT) as well as 73 surgically differently prepared pigs bacterial fractionations and 2.6-diaminopimelic acid (DAP) estimations were carried out in order to calculate the bacterial N proportion in faeces N and digesta N after feeding various diets. Because of a high individual variability and the analytical variation width of the DAP/N-ratios no distinct influences of the fed diets could be found. The average DAP/N-values in the faeces (0.224) of INT pigs ranged in the same magnitude as in the digesta (0.0272) of ileorectostomized (IRA) pigs with open colon descendens (IRAo), where a digesta backflow is possible. Distinct lower DAP/N-ratios (0.0125 resp. 0.0043), however, were found in the digesta of pigs with ileo-caecal cannulae (IZB) or IRA pigs with closed colon (IRAg). On the base of various premises (N of the "bacterial fraction" C is only bacterial N; the DAP found in fraction A originates from intestinal bacteria adhering to feed particles) conducted calculations of the bacterial N proportions (in per cent of total N) led to the following data: Faeces of INT pigs: 43.0 ... 68.2 vs. 69.6 ... 89.0; digesta of operated pigs (except protein free diet) IRAo: 22.3 ... 57.0 vs. 46.2 ... 73.8; IZB: 17.0 ... 35.7 vs. 25.2 ... 53.6; IRAg (only 3 pigs): 23.6 vs. 24.2. The proportion of bacterial N in the digesta N of protein free fed IRAo pigs was 22.0 vs. 22.6%.
Wünsche
J
J
Forschungszentrum für Tierproduktion Dummersdorf-Rostock, Bereich Tierenährung Oskar Kellner.
Völker
T
T
Souffrant
W B
WB
Borgmann
E
E
D000821:Animal Feed; D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D003960:Diaminopimelic Acid / Q000032:analysis; D005243:Feces / Q000737:chemistry*; D005766:Gastrointestinal Contents / Q000737:chemistry; D007082:Ileum / Q000737:chemistry* / Q000382:microbiology; D009584:Nitrogen / Q000032:analysis*; D013552:Swine / Q000382:microbiology*
D003960:Diaminopimelic Acid; D009584:Nitrogen
Bestimmung der Bakterien-N-Anteile im Kot und unterschiedlich gewonnenem Ileumchymus von Schweinen.