In vivo inhibition of megakaryocyte and platelet production by platelet factor 4 in mice.
The in vivo effect of human platelet factor 4 (PF4) on murine megakaryocytopoiesis and thrombopoiesis was studied. Administration of PF4 induced a dose-dependent decrease in the numbers of megakaryocytes and their progenitor cells (CFU-MK), continuing for 1 week after the injection. However, the size of megakaryocytes and their colonies was not changed following PF4 injection. Platelet levels were significantly decreased at days 3-4. The number of CFU-GM was decreased at days 1-2. White blood cells and hemoglobin were unaffected by PF4. These data indicate that PF4 inhibits megakaryocyte and platelet production in vivo by acting on the early stage of megakaryocyte development.
Han
Z C
ZC
Institut des Vaisseaux et du Sang, I.N.S.E.R.M. U n. 150, Hôpital Lariboisière, Paris.
Bellucci
S
S
Bodevin
E
E
Wan
H Y
HY
Shen
Z X
ZX
Caen
J P
JP
D000818:Animals; D002452:Cell Count / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006412:Hematopoietic Stem Cells / Q000166:cytology; D008297:Male; D008533:Megakaryocytes / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010976:Platelet Count / Q000187:drug effects; D010978:Platelet Factor 4
D010978:Platelet Factor 4