title: Preliminary studies on quinoprotein glucose dehydrogenase under extreme conditions of temperature and pressure.
abstract: The kinetics of the reduction of the quinoprotein glucose dehydrogenase by substrate were studied as a function of 3 parameters: pressure (1-1000 bar), temperature (down to -25 degrees C) and solvent (water and 40% dimethyl sulfoxide, DMSO) using a high-pressure low-temperature stopped-flow apparatus. A 2-step formation of the reduced enzyme by its substrate (xylose), was observed. A rapid equilibrium described by the constant K1 was followed by a slower process described by the constants k2 and k-2. By using the transition state theory, the thermodynamic quantities delta V (activation volumes) were determined for these various kinetics constants under different experimental conditions. The results are discussed in terms of conformational change and solvation effect on the protein shell, and compared with results obtained for other systems as the 2-step formation of horseradish peroxidase compound I.
authors.affiliation: Department of Microbiology and Enzymology, Delft University of Technology, The Netherlands.
authors.forename: J F
authors.initials: JF
authors.lastname: Jzn
authors.forename: J A
authors.initials: JA
authors.lastname: Duine
authors.forename: C
authors.initials: C
authors.lastname: Balny
mesh_terms: D005948:Glucose Dehydrogenases / Q000378:metabolism*; D007700:Kinetics; D011312:Pressure; D013696:Temperature; D014994:Xylose
chemical_list: D014994:Xylose; D005948:Glucose Dehydrogenases; C033955:glucose dehydrogenase (pyrroloquinoline-quinone)