A study of blood antioxidants and lipoperoxides: effects of anticoagulants and sex-variation.
To study the influences of anticoagulants and sex-related variation on antioxidants, such as vitamins A and E, beta-carotene, and uric acid; and lipid peroxides such as malondialdehyde (MDA), we collected samples from 43 human subjects for research. In this study, it was found that: There were no differences of vitamin E and MDA concentrations and the ratio for tocopherol/total lipids among plasma from liquid EDTA(K3) blood and lithium heparin, or serum (p greater than 0.05). Nevertheless, plasma from liquid EDTA(K3) blood had significantly lower vitamin A. beta-carotene, uric acid, total cholesterol, triglyceride, and total lipids concentrations than did serum or plasma from blood treated with lithium heparin (p less than 0.01). The possible mechanism which reduced the above analytes was either EDTA-induced deterioration or oxidation. In sex-related variation study, sex differences in the plasma levels of vitamins A and E were insignificant (p greater than 0.05). Higher levels of plasma beta-carotene values were found in females (p less than 0.01); whereas, higher levels of plasma uric acid and MDA concentrations were found in males (p less than 0.01).
Tsai
L Y
LY
School of Technology for Medical Sciences, Kaohsiung Medical College, Taiwan, Republic of China.
Tsai
S M
SM
Horng
N C
NC
D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism*; D002338:Carotenoids / Q000097:blood; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008054:Lipid Peroxides / Q000097:blood*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D012737:Sex Factors; D014527:Uric Acid / Q000097:blood; D014801:Vitamin A / Q000097:blood; D014810:Vitamin E / Q000097:blood; D019207:beta Carotene
D000925:Anticoagulants; D000975:Antioxidants; D008054:Lipid Peroxides; D019207:beta Carotene; D014801:Vitamin A; D014810:Vitamin E; D014527:Uric Acid; D002338:Carotenoids; D008315:Malondialdehyde; D006493:Heparin; D004492:Edetic Acid