[A rapid method of preparing the (H3-H4-H2A-H2b)(2) histone octamer in large quantities].
A simple and fast method for isolation of large amounts of the histone octamer (H2A-H2B-H3-H4)2 is proposed. This method is based on chromatin adsorption by hydroxyapatite with subsequent extraction of the histone octamer with 50 mM sodium-phosphate buffer containing 4 M NaCl pH 8.0. It was shown that the properties of the histone octamer isolated by this extractive procedure are identical with those of the histone octamer obtained by elution on a Sephadex G-100 column. The histone tetramer (H3-H4)2 and dimer (H2A-H2B) were obtained after gel filtration on Sephadex G-100 in 50 mM sodium-acetate (pH 5.6).
Tiulenev
V I
VI
Konoplich
L A
LA
Krivonos
A A
AA
Khrapunov
S N
SN
D000818:Animals; D002417:Cattle; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000737:chemistry; D002843:Chromatin / Q000737:chemistry; D002850:Chromatography, Gel; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000737:chemistry / Q000302:isolation & purification*; D011487:Protein Conformation
D002843:Chromatin; D006657:Histones; D004247:DNA
Bystryĭ metod polucheniia oktamera (H3-H4-H2A-H2B)(2) gistonov v bol'shikh kolichestvakh.