Synthetic antigens as immunogens: Part IV. Specificity of antisera developed to Gal beta 1-3(GlcNAc beta 1-6)GalNAc and GlcNAc beta 1-6GalNAc.
Antisera to the chemically synthesized trisaccharide, Gal beta 1-3(GlcNAc beta 1-6)GalNAc, and disaccharide, GlcNAc beta 1-6GalNAc, were developed using the diazophenyl bovine serum albumin derivatives. The binding specificity of the antisera were analyzed by enzyme immunoassays with structurally related, chemically synthesized oligosaccharides. The anti-trisaccharide antibody showed no reactivity to T antigenic structures which bear the Gal beta 1-3GalNAc moiety. The immunodominant area of the Gal beta 1-3(GlcNAc beta 1-6)GalNAc was contained in the GlcNAc beta 1-6GalNAc region of the molecule. The reactivity pattern of the anti-trisaccharide antibody, although directed to the disaccharide, did not exactly duplicate the reactivity pattern of the anti-disaccharide.
Matta
K L
KL
Department of Gynecologic Oncology, Roswell Park Cancer Institute, Buffalo, NY 14263.
Piskorz
C F
CF
Abbas
S A
SA
Diakun
K R
KR
D000918:Antibody Specificity; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000276:immunology*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D012710:Serum Albumin, Bovine; D014312:Trisaccharides / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*
D004187:Disaccharides; D014312:Trisaccharides; D014614:Vaccines, Synthetic; C073214:N-acetylglucosaminyl beta(1-6)N-acetylgalactosamine; D012710:Serum Albumin, Bovine; C073212:galactosyl beta(1-3)-N-acetylglucosaminyl-beta(1-6)-N-acetylgalactosamine