Calretinin, a neuronal calcium binding protein, inhibits phosphorylation of a 39 kDa synaptic membrane protein from rat brain cerebral cortex.
The neuronal calcium binding protein calretinin was studied for possible effects on brain protein phosphorylation. Calretinin (100 nM) inhibited the appearance of a calcium stimulated 39 kDa phosphoprotein within a synaptic membrane fraction following sucrose density centrifugation. Calmodulin or a specific protein kinase C inhibitor had no effect on either the phosphorylation of the 39 kDa protein or the inhibition produced by calretinin. At the same concentration, calretinin produced a slight increase in the phosphorylation of several other synaptic membrane proteins which appeared additive with the stimulation produced by either calmodulin or phosphatidylserine in the presence of calcium.
Yamaguchi
T
T
Laboratory of Clinical Science, NIMH, Bethesda, MD 20892.
Winsky
L
L
Jacobowitz
D M
DM
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D064032:Calbindin 2; D002147:Calmodulin / Q000494:pharmacology; D002540:Cerebral Cortex / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism* / Q000494:pharmacology; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D051381:Rats; D064030:S100 Calcium Binding Protein G / Q000494:pharmacology*; D013570:Synaptic Membranes / Q000378:metabolism*
C576512:Calb2 protein, rat; D064032:Calbindin 2; D002147:Calmodulin; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; D064030:S100 Calcium Binding Protein G; D000255:Adenosine Triphosphate