title: Cloning and expression of penicillin G acylase gene in Bacillus megaterium.
abstract: Bacillus megaterium BM1, which produces penicillin G acylase (PGA), has been isolated. Gene encoding for PGA was cloned into E. coli MC1061 using pBR322 as the vector, obtaining a recombinant plasmid pBmPA4 containing 9.9 kb inserted DNA. Restriction map of the plasmid was analyzed. A pBmPA5 containing 4.9 kb was gained by deletion in vitro. Both pBmPA4 and pBmPA5 clones can be expressed in E.coli MC1061, and their expressions were induced by phenylacetic acid.
authors.affiliation: Institute of Biophysics, Chinese Academy of Sciences, Beijing.
authors.forename: L F
authors.initials: LF
authors.lastname: Zhang
authors.forename: Z W
authors.initials: ZW
authors.lastname: Li
authors.forename: Q J
authors.initials: QJ
authors.lastname: Zhang
mesh_terms: D001410:Bacillus megaterium / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D010399:Penicillin Amidase / Q000235:genetics*; D010957:Plasmids; D015183:Restriction Mapping
chemical_list: D010399:Penicillin Amidase