[Species-specific features of the distribution of restriction sites in Bsp-repeats of Canidae genome].
Differences and similarities of the Bsp-repeats' organization in fox, dog, polar fox and raccoon dog genomes were studied. Specificity of Bsp-repeats to the Canidae family was demonstrated. The repeats are mainly organized in large clusters in all species studied. The species-specific features in restriction patterns were revealed for all five genomes, in spite of high intragenomic polymorphism exhibited in each case. This suggests that certain unique sets of structural versions of Bsp-repeats were fixed in canid genomes by amplification during the process of speciation. Fox and polar fox exhibited the highest similarity in restriction patterns of Bsp-repeats. Raccoon dog pattern is most unusual among others: its distinguishable character is the absence of large multimeric series. The EcoRI hydrolysate of raccoon dog Bsp-repeats consists mainly of one band corresponding to 1600 bp. These are in accordance with phylogenetic relations between canids.
Ivanov
S V
SV
Potapov
V A
VA
Filipenko
E A
EA
Romashchenko
A G
AG
D000818:Animals; D002335:Carnivora / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D016678:Genome; D015151:Immunoblotting; D011110:Polymorphism, Genetic / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D015183:Restriction Mapping; D013045:Species Specificity
D004247:DNA
Vidospetsificheskie osobennosti raspredeleniia restriktsionnykh saĭtov v Bsp-povtorakh genomov Canidae.