Nucleotide sequence of a Lactococcus lactis gene cluster encoding adenylate kinase, initiation factor 1 and ribosomal proteins.
We have previously isolated a putative promoter from the Lactococcus lactis subsp. lactis chromosome. We now report the sequence of the promoter fragment and its extension in the 5'-direction. The region contains several open-reading frames which correspond to ribosomal protein L15, SecY, adenylate kinase, initiation factor 1 and ribosomal proteins B and S13. The order of the genes, rplO (L15), secY, adk, infA, rpmJ (B) and rpsM (S13), is similar to that in the spc and alpha operon region of Bacillus subtilis, with the exception of the map gene, coding for methionine amino peptidase, which is located between adk and infA in B. subtilis. The putative promoter is located between adk and infA.
Koivula
T
T
Institute of Biotechnology, University of Helsinki, Finland.
Hemilä
H
H
D000263:Adenylate Kinase / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D015977:Eukaryotic Initiation Factor-1 / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D013294:Lactococcus lactis / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009876:Operon; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism
D004269:DNA, Bacterial; D015977:Eukaryotic Initiation Factor-1; D012269:Ribosomal Proteins; D000263:Adenylate Kinase