Surface properties of intraocular lens materials and their influence on in vitro cell adhesion.
An in vitro model to assess lens epithelial cell adhesion to a variety of intraocular lens materials was developed. Rabbit anterior lens capsules were isolated and cultured in serum-containing medium. Test surfaces included poly(methyl methacrylate), two new silicones (SLM-1/UV, SLM-2/UV), two hydrogels (HEMA, Lidofilcon A), and polytetrafluoroethylene (PTFE). Following the application and culturing of cells on the test surfaces, adherent cells were removed by trypsinization and counted at eight and 24 hours. The material surfaces were characterized by electron spectroscopy for chemical analysis and scanning electron microscopy. The captive bubble technique was also used to assess interfacial free energy. More cells adhered to PMMA than to the other materials tested (P less than .01). The two silicones, HEMA, and PTFE did not differ significantly from each other; Lidofilcon A had the lowest cell adhesion of all materials tested. Cell adhesion results were related to the interfacial free energy of each material. Materials of low (less than 5 ergs/cm2) or high (greater than 40 ergs/cm2) interfacial free energies had lower cell adhesion than materials of intermediate free energies (5 to 40 ergs/cm2) which exhibited the highest cell adhesion.
Cunanan
C M
CM
Research Department, Allergan Medical Optics, Irvine, California 91218.
Tarbaux
N M
NM
Knight
P M
PM
D000818:Animals; D002448:Cell Adhesion; D002452:Cell Count; D002478:Cells, Cultured; D004848:Epithelium / Q000648:ultrastructure; D007903:Lens Capsule, Crystalline / Q000166:cytology* / Q000502:physiology; D007910:Lenses, Intraocular; D008689:Methacrylates; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry; D011138:Polytetrafluoroethylene; D011817:Rabbits; D013499:Surface Properties
D008689:Methacrylates; D011108:Polymers; C005044:hydroxyethyl methacrylate; D011138:Polytetrafluoroethylene