Human manganese superoxide dismutase is readily detectable by a copper blotting technique.
Manganese-superoxide dismutase (MnSOD) in human fibroblast and liver lysates was found to bind copper avidly under the conditions of a copper blotting technique which also detects known copper-binding proteins. Competition studies suggest that the copper-binding site of the molecule under these conditions is distinct from its manganese-binding site. Copper blotting provides a sensitive way to detect MnSOD in human tissues, and may be generally applicable to studies of copper-binding by biological molecules.
Kaler
S G
SG
Human Genetics Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
Maraia
R J
RJ
Gahl
W A
WA
D000595:Amino Acid Sequence; D001345:Autoradiography / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D003300:Copper; D003301:Copper Radioisotopes; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000302:isolation & purification; D013482:Superoxide Dismutase / Q000032:analysis*
D003301:Copper Radioisotopes; D007527:Isoenzymes; D010446:Peptide Fragments; D003300:Copper; D013482:Superoxide Dismutase; C029892:cupric chloride