Structural comparison of the Autographa californica nuclear polyhedrosis virus-induced RNA polymerase and the three nuclear RNA polymerases from the host, Spodoptera frugiperda.
A partial subunit structure has been determined for the novel RNA polymerase that is induced in fall armyworm (Spodoptera frugiperda) cells upon infection with the Autographa californica nuclear polyhedrosis virus (AcNPV). The putative structure includes nine polypeptides; the complexity of this structure is in accord with the high sedimentation coefficient (15S) estimated for this enzyme. A comparison of the putative structure of the virus-induced polymerase with those of the three host nuclear RNA polymerases shows that the structure of the viral polymerase is apparently unlike any of the host nuclear polymerases. This conclusion is reinforced by immunoblot experiments that show no cross-reactivity between the virus-induced polymerase and an antiserum directed against Drosophila RNA polymerase II. The virus-induced RNA polymerase appears at the onset of the late phase of infection and still appears when viral DNA synthesis is blocked by aphidicolin. Thus, the virus-induced polymerase seems to be composed of early viral products.
Yang
C L
CL
Department of Biochemistry, University of Kansas, Lawrence 66045.
Stetler
D A
DA
Weaver
R F
RF
D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D016367:Baculoviridae / Q000201:enzymology*; D002499:Centrifugation, Density Gradient; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D015342:DNA Probes; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000187:drug effects / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D009036:Moths / Q000201:enzymology*; D015138:RNA, Nuclear / Q000737:chemistry* / Q000302:isolation & purification; D012367:RNA, Viral / Q000737:chemistry* / Q000187:drug effects / Q000302:isolation & purification; D013997:Time Factors; D014158:Transcription, Genetic
D015342:DNA Probes; D015138:RNA, Nuclear; D012367:RNA, Viral; D016590:Aphidicolin; D012321:DNA-Directed RNA Polymerases