Effect of phosphoenolpyruvate on energy metabolism of ischemic liver in anesthetized rats--31P-MRS study.
The effect of phosphoenolpyruvate (PEP) on energy metabolism of ischemic liver was examined in anesthetized rats. In vivo 31P-NMR spectroscopy (31P-MRS) was used to monitor cellular energy metabolism. Hepatic ischemia was induced by temporarily clamping the portal vein for 60 minutes. The liver adenosine triphosphate (ATP) levels decreased remarkably during ischemia, and they gradually increased after ischemia but did not return to pre-operative levels. PEP effectively increased the levels of ATP. The ATP levels of the PEP-treated rats were significantly higher than those of the control rats, and also intracellular acidosis was improved during post-ischemic reperfusion. These findings suggest that PEP may have a cytoprotective effect and improve the energy metabolism in the ischemic liver.
Matsui
K
K
Osaka-Higashi Circulation Hospital, Japan.
Kojima
M
M
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000758:Anesthesia; D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects*; D006019:Glycolysis / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007511:Ischemia / Q000378:metabolism*; D008099:Liver / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010728:Phosphoenolpyruvate / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000517:prevention & control
D010728:Phosphoenolpyruvate; D000255:Adenosine Triphosphate