Identification of rat cDNA encoding hck tyrosine kinase from megakaryocytes.
A rat homologue of hck tyrosine kinase cDNA from a rat megakaryocyte library contains 1911 nucleotides with an open reading frame encoding 503 amino acids. The rat hck had distinct amino acid residues from the mouse homologue exhibiting 97.6% identity. The sequence contains the SH2 and SH3 regions that interact with cytoplasmic signaling proteins, the kinase domain including the nucleotide binding site and the autophosphorylation site, and the C-terminal Tyr-499 known as a negative regulator.
Okano
Y
Y
Department of Biochemistry, Gifu University School of Medicine, Japan.
Sugimoto
Y
Y
Fukuoka
M
M
Matsui
A
A
Nagata
K
K
Nozawa
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D002872:Chromosome Deletion; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D006801:Humans; D008533:Megakaryocytes / Q000201:enzymology*; D051379:Mice; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D004247:DNA; D011505:Protein-Tyrosine Kinases