Direct determination of crystallographic phases for diffraction data from lipid bilayers. II. Refinement of phospholipid structures.
Using a systematic approach for the acceptance of crystallographic phase assignment, based on the evaluation of triplet structure invariants, electron and x-ray diffraction data from phospholipid multilamellar arrays are analyzed by direct methods. After calculation of Fourier maps with a partial set of phased structure factor magnitudes, the structure is refined in real space by flattening of the hydrocarbon region of the bilayer and an optimal solution is sought either by the calculation of [delta rho 4] suggested by Luzzati, where rho is the structure density or by a test of density smoothness [magnitude of delta rho/ delta r magnitude of], where r positions are located along the normal to the lamellar surface. Reanalyses of previously determined structures sometimes lead to new conclusions (e.g., a possible similarity of the electron density profile for DL-DMPE and L-DMPE, and a clear indication of the fatty acid adduct in the mixed L-DPPC/palmitic acid bilayer). Because of presumed secondary scattering perturbations (primarily to the least intense reflections), the refinements of the electron diffraction intensities are less easily evaluated than those carried out with x-ray diffraction data.
Dorset
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Electron Diffraction Department, Medical Foundation of Buffalo, Inc., New York 14203.
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D003461:Crystallography / Q000379:methods; D004583:Electrons; D008051:Lipid Bilayers / Q000737:chemistry*; D008433:Mathematics; D008968:Molecular Conformation; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D011199:Potentiometry; D013329:Structure-Activity Relationship; D014961:X-Ray Diffraction / Q000379:methods
D008051:Lipid Bilayers; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D015060:1,2-Dipalmitoylphosphatidylcholine; C018525:1,2-dimyristoylphosphatidylethanolamine