High-performance liquid chromatography of urinary oligosaccharides in the diagnosis of glycoprotein degradation disorders.
Urinary oligosaccharides can be separated by high-performance anion-exchange chromatography using a Dionex CarboPac PA1 column, elution with aqueous sodium hydroxide and sodium acetate solutions and detection by pulsed amperometry. Each of the urines of patients with glycoprotein degradation disorders yielded a pattern of oligosaccharide excretion unique for that disorder, facilitating an unambiguous diagnosis. The method is sensitive (10 microliters of urine required) and fast (40 min).
Hommes
F A
FA
Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta 30912-2100.
Varghese
M
M
D002239:Carbohydrate Metabolism, Inborn Errors / Q000175:diagnosis / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016537:Gangliosidosis, GM1 / Q000652:urine; D006801:Humans; D009844:Oligosaccharides / Q000652:urine*; D001616:beta-Galactosidase / Q000172:deficiency
D009844:Oligosaccharides; D001616:beta-Galactosidase