A sensitive multienzymatic assay for the measurement of pyruvate, dihydroxyacetone phosphate, oxaloacetate, and acetoacetate in clear extracts from biological samples.
A multienzymatic method for the measurement of pyruvate, dihydroxyacetone phosphate, oxaloacetate, and acetoacetate is presented. The determination procedure is considered suitable because it is simple, sensitive, and its advantages could be demonstrated by comparison with the original methods.
Arias-Mendoza
F
F
Biochemistry Department, School of Medicine, National University of Mexico, D.F.
Piña
E
E
D000090:Acetoacetates / Q000032:analysis*; D004099:Dihydroxyacetone Phosphate / Q000032:analysis*; D008722:Methods; D009243:NAD / Q000378:metabolism; D010071:Oxaloacetates / Q000032:analysis*; D010084:Oxidation-Reduction; D010088:Oxidoreductases; D011773:Pyruvates / Q000032:analysis*; D013053:Spectrophotometry
D000090:Acetoacetates; D010071:Oxaloacetates; D011773:Pyruvates; D009243:NAD; C016635:acetoacetic acid; D004099:Dihydroxyacetone Phosphate; D010088:Oxidoreductases