[Polyphotobiotin--a new reagent for introducing biotin into nucleic acids].
A new reagent, polyphotobiotin (PPhB), for labelling DNA probes has been obtained using low molecule oligoethylenimine (M 600 Da). Photoreactive group of PPhB is 4-azido-salicylic acid. The procedure is simple and quick, the reaction time being about 20 min. PPhB-labelled DNA has the same efficiency in the hybridisation analysis as DNA labelled with Bio-4-dUTP via PCR.
Turchinskiĭ
M F
MF
Shcherbo
S N
SN
Knorre
V D
VD
Kolesnik
T B
TB
Aleĭnik
Iu V
IuV
D000345:Affinity Labels; D001386:Azides / Q000737:chemistry*; D001435:Bacteriophages / Q000378:metabolism; D001710:Biotin / Q000031:analogs & derivatives* / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D000345:Affinity Labels; D001386:Azides; D015342:DNA Probes; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D009696:Nucleic Acids; D011108:Polymers; D001710:Biotin; C045549:photobiotin
Polifotobiotin--novyĭ reagent dlia vvedeniia biotina v nukleinovye kisloty.