Metabolism of fructose-1,6-bisphosphate by mature boar spermatozoa.
Mature epididymal boar spermatozoa converted glucose and fructose to carbon dioxide and lactate and maintained high concentrations of ATP. In the presence of (S)-alpha-chlorohydrin these processes were inhibited and there was an accumulation of fructose-1,6-bisphosphate and dihydroxyacetone phosphate. With fructose-1,6-bisphosphate as the substrate, the concentration of ATP was maintained, carbon dioxide was evolved and dihydroxyacetone phosphate accumulated. Cells pre-incubated with (S)-alpha-chlorohydrin did not maintain ATP levels, evolved less carbon dioxide and produced dihydroxyacetone phosphate. Assays of incubates in which fructose-1,6-bisphosphate was used as the substrate showed the presence of equilibrium quantities of fructose-6-phosphate and glucose-6-phosphate which were not detected when either fructose or glucose were used as substrates. [14C]Fructose and [14C]glucose were not produced from [14C]fructose-1,6-bisphosphate in spermatozoal incubates which had or had not been pre-incubated with (S)-alpha-chlorohydrin. Evidence is presented that a high concentration of fructose-1,6-bisphosphate leads to the formation of fructose-6-phosphate and glucose-6-phosphate but not of fructose and/or glucose.
Jones
A R
AR
Department of Biochemistry, University of Sydney, NSW, Australia.
Montague
M D
MD
D000244:Adenosine Diphosphate / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002245:Carbon Dioxide / Q000032:analysis; D004099:Dihydroxyacetone Phosphate; D005632:Fructose / Q000032:analysis; D005635:Fructosediphosphates / Q000378:metabolism*; D005636:Fructosephosphates / Q000032:analysis; D005947:Glucose / Q000032:analysis; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000032:analysis; D008297:Male; D013094:Spermatozoa / Q000378:metabolism*; D013552:Swine; D000517:alpha-Chlorohydrin / Q000494:pharmacology
D005635:Fructosediphosphates; D005636:Fructosephosphates; D005958:Glucosephosphates; D002245:Carbon Dioxide; D005632:Fructose; D019298:Glucose-6-Phosphate; D004099:Dihydroxyacetone Phosphate; D000244:Adenosine Diphosphate; C027618:fructose-6-phosphate; D000255:Adenosine Triphosphate; D000517:alpha-Chlorohydrin; D005947:Glucose; C029063:fructose-1,6-diphosphate