Toxicity of sera from individuals with Chagas' disease to cultured rat embryos: role of antibodies to laminin.
In previous studies antilaminin antibodies in the sera of immunized monkeys and rats were found to be toxic to cultured rat embryos. In order to extend these studies to humans, head-fold stage rat embryos were cultured for 48 hours on ten different serum samples from individuals with Chagas' disease. All embryos (n = 20) cultured on these sera were found to be abnormal. Using ELISA, Western immunoblot, and indirect immunofluorescence it could be shown that antibodies in these sera reacted with laminin. That these antilaminin antibodies were, at least in part, responsible for the toxicity was indicated 1) by reduced cultured embryo toxicity for six of seven serum samples after pre-absorption with purified laminin, 2) by demonstrating a relationship between the amount of affinity-purified antilaminin IgG added to control serum for culture and the severity of embryo abnormalities seen at the end of culture, and 3) by the sera's failing to react with other basement membrane proteins, including type IV collagen, fibronectin, osteonectin, and heparan sulfate proteoglycan.
Robbins
B
B
Department of Animal Science, University of Connecticut, Storrs 06269-4039.
Klein
N W
NW
Cavalcanti
H
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000906:Antibodies / Q000633:toxicity*; D000937:Antigen-Antibody Reactions / Q000276:immunology; D014355:Chagas Disease / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003470:Culture Media; D004622:Embryo, Mammalian / Q000187:drug effects* / Q000473:pathology; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000633:toxicity*; D007223:Infant; D007797:Laminin / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009924:Organ Culture Techniques; D051381:Rats
D000906:Antibodies; D003470:Culture Media; D007106:Immune Sera; D007797:Laminin