title: Plasma desorption mass spectrometry of haemoglobin tryptic peptides for the characterization of a Hungarian alpha-chain variant.
abstract: S-Aminoethylated-alpha A and -beta A globin tryptic peptides separated by reversed-phase high-performance liquid chromatography have been analysed by plasma desorption mass spectrometry. Almost all the expected alpha A and beta A tryptic fragments were tentatively assigned relative to the known globin chain sequences based on the molecular weight obtained by plasma desorption mass spectrometric analysis of the purified peptides. The application of plasma desorption mass spectrometry for structure elucidation of a haemoglobin alpha-chain variant revealed the first case of Hb Hasharon in Hungary.
authors.affiliation: Department of Molecular Biology, Odense University, Denmark.
authors.forename: O N
authors.initials: ON
authors.lastname: Jensen
authors.forename: P
authors.initials: P
authors.lastname: Roepstorff
authors.forename: B
authors.initials: B
authors.lastname: Rozynov
authors.forename: M
authors.initials: M
authors.lastname: Horanyi
authors.forename: J
authors.initials: J
authors.lastname: Szelenyi
authors.forename: S R
authors.initials: SR
authors.lastname: Hollan
authors.forename: E A
authors.initials: EA
authors.lastname: Aseeva
authors.forename: V A
authors.initials: VA
authors.lastname: Spivak
mesh_terms: D000328:Adult; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D006814:Hungary; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000032:analysis
chemical_list: D006455:Hemoglobins, Abnormal; D010455:Peptides; C029985:hemoglobin Sealy