Dialysis membranes decrease immunoglobulin and interleukin-6 production by peripheral blood mononuclear cells in vitro.
In order to determine the influence of dialysis membranes on immunoglobulin (Ig) and interleukin-6 (IL-6) production, peripheral blood mononuclear cells (PBMC) from 11 haemodialysis patients were cultured for 7 days on Cuprophan, Hemophan, and polyacrylonitrile flat-sheet dialysis membranes. IL-6, IgG, IgA, and IgM were assayed in the supernatants using ELISA. Pokeweed-mitogen-stimulated IgG production declined significantly from 319 +/- 40 ng/ml on polystyrole to 162 +/- 26 ng/ml on Cuprophan, 135 +/- 25 ng/ml on Hemophan, and 109 +/- 20 ng/ml on polyacrylonitrile. A similar pattern was observed for IgA production by PBMC. In comparison to polystyrole (724 +/- 34 pg/ml), IL-6 production by PBMC was significantly reduced in the presence of Cuprophan (151 +/- 45 pg/ml), Hemophan (167 +/- 6 pg/ml) and polyacrylonitrile (108 +/- 33 pg/ml). The fact that the level of monocyte-derived IL-6, a stimulator of B cells, was decreased suggests that reduced B cell activity may be due to diminished stimulation by monocytes.
Paczek
L
L
Department of Internal Medicine, University of Würzburg, Germany.
Schaefer
R M
RM
Heidland
A
A
D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D066298:In Vitro Techniques; D015850:Interleukin-6 / Q000096:biosynthesis*; D007676:Kidney Failure, Chronic / Q000276:immunology / Q000628:therapy; D007688:Kidneys, Artificial; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology; D006435:Renal Dialysis / Q000009:adverse effects*
D007136:Immunoglobulins; D015850:Interleukin-6; D008567:Membranes, Artificial