title: bimA encodes a member of the tetratricopeptide repeat family of proteins and is required for the completion of mitosis in Aspergillus nidulans.
abstract: The recessive, temperature-sensitive bimA1 mutation of Aspergillus nidulans blocks nuclei in metaphase at restrictive temperature. To determine whether the bimA product is essential, integrative transformation was used to create a mutation in the bimA gene. The mutation was maintained in a heterokaryon and the phenotype of spores produced by the heterokaryon was analyzed. Molecular disruption of the wild-type bimA gene is recessive in the heterokaryon and causes a metaphase block, demonstrating that bimA is an essential gene for mitosis. bimA was cloned by DNA-mediated complementation of its mutant phenotype at restrictive temperature, and the nucleotide sequence of a full-length cDNA was determined. A single large open reading frame was identified in the cDNA sequence, which predicts a protein containing 806 amino acid residues that is related (30.4% identity) to the Schizosaccharomyces pombe nuc2+ gene product, which also is required for completion of mitosis. The sequence of the bimA gene indicates that it is a member of a family of mostly nuclear proteins that contain a degenerate 34 amino acid repeat, the TPR (tetratricopeptide repeat) gene family.
authors.affiliation: Department of Pharmacology, University of Medicine and Dentistry of New Jersey, Piscataway 08854.
authors.forename: K L
authors.initials: KL
authors.lastname: O'Donnell
authors.forename: A H
authors.initials: AH
authors.lastname: Osmani
authors.forename: S A
authors.initials: SA
authors.lastname: Osmani
authors.forename: N R
authors.initials: NR
authors.lastname: Morris
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000166:cytology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D005808:Genes, Recessive; D005816:Genetic Complementation Test; D008677:Metaphase; D008938:Mitosis; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D013696:Temperature
chemical_list: D005656:Fungal Proteins; D009687:Nuclear Proteins; C072411:BIMA protein, Emericella nidulans