Thin-layer chromatographic detection of glucocorticoids.
An adsorption thin-layer chromatography (TLC) is described for the detection of glucocorticoids including triamcinolone, prednisolone, 6 alpha-methylprednisolone, betamethasone, dexamethasone, hydrocortisone, prednisone and cortisone. Separation of the glucocorticoids was performed on a silica stationary phase with chloroform-dioxane-methanol (15:4:1, v/v) as a mobile phase. The complete resolution of the epimers of betamethasone and dexamethasone was attained by derivatization of them with (1S)-(-)-camphanic chloride using triethylamine as a catalyst. The application of the method to the analysis of glucocorticoids in various samples is being smoothly developed.
Wu
S M
SM
School of Pharmacy, Kaohsiung Medical College, Taiwan, Republic of China.
Chen
S H
SH
Wu
H L
HL
D001623:Betamethasone / Q000032:analysis; D002855:Chromatography, Thin Layer; D003907:Dexamethasone / Q000032:analysis; D005938:Glucocorticoids / Q000032:analysis*; D013058:Mass Spectrometry
D005938:Glucocorticoids; D003907:Dexamethasone; D001623:Betamethasone