The involvement of polymorphonuclear leukocytes in the pathogenesis of bronchopneumonia in calves. II. Granulocyte-induced changes in erythrocyte membrane phospholipid topology.
Phospholipid topology in erythrocyte membranes of bronchopneumonic calves and changes in phospholipid asymmetry induced by incubation of erythrocytes with neutrophils of diseased calves were examined. Using aminophospholipid tracing by non-penetrating probe, trinitrobenzenesulfonic acid, and phospholipid hydrolysis by specific phospholipase A2 from Naja naja venom and sphingomyelinase from Staphylococcus aureus it was stated that in erythrocytes from diseased calves phosphatidylserine and, to some extent, phosphatidylethanolamine are externalized on the outer leaflet of the membrane. Similar results were obtained for erythrocytes of healthy calves after incubation with granulocytes of bronchopneumonic animals. It is suggested that granulocytes of bronchopneumonic calves cause perturbations in erythrocyte membrane phospholipid organization.
Ledwozyw
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A
Department of Pathophysiology, Veterinary Faculty, Agricultural Academy, Lublin, Poland.
Stolarczyk
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H
D000818:Animals; D001996:Bronchopneumonia / Q000209:etiology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000209:etiology*; D004910:Erythrocyte Membrane / Q000737:chemistry*; D008315:Malondialdehyde / Q000378:metabolism; D009504:Neutrophils / Q000502:physiology*; D010743:Phospholipids / Q000032:analysis*
D010743:Phospholipids; D008315:Malondialdehyde