[Localization of fragments binding nuclear proteins in the promotor region of the human alpha-1-antitrypsin gene].
Human and mice nuclear extracts from livers and mice spleen extract were analysed in an attempt to find any proteins capable of binding to the human alpha 1-antitrypsin gene promoter. The nuclei of all studied tissues contain such proteins. The proteins were partially purified on DEAE-trisacryl, heparin sepharose and phosphocellulose columns. The multiple sites for liver nuclear proteins binding to the human alpha 1-antitrypsin gene promoter were found by the DNAse I footprinting technique.
Neznanov
N S
NS
Makarova
I V
IV
Gazarian
K G
KG
D000818:Animals; D001483:Base Sequence; D006801:Humans; D008099:Liver / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D013154:Spleen / Q000378:metabolism; D013379:Substrate Specificity; D000515:alpha 1-Antitrypsin / Q000235:genetics* / Q000378:metabolism
D009687:Nuclear Proteins; D000515:alpha 1-Antitrypsin
Lokalizatsiia v promotornoĭ oblasti gena alpha-1-antitripsina cheloveka uchastkov sviazyvaniia s iadernymi belkami.