title: Identification of mycobacteria by high-performance liquid chromatography.
abstract: Mycolic acids extracted from saponified mycobacterial cells were examined as p-bromophenacyl esters by high-performance liquid chromatography (HPLC). Standard HPLC patterns were developed for species of Mycobacterium by examination of strains from culture collections and other well-characterized isolates. Relative retention times of peaks and peak height comparisons were used to develop a differentiation scheme that was 98% accurate for the species examined. A rapid, cost-effective HPLC method which offers an alternative approach to the identification of mycobacteria is described.
authors.affiliation: Division of Bacterial and Mycotic Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
authors.forename: W R
authors.initials: WR
authors.lastname: Butler
authors.forename: K C
authors.initials: KC
authors.lastname: Jost
authors.forename: J O
authors.initials: JO
authors.lastname: Kilburn
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005069:Evaluation Studies as Topic; D006801:Humans; D009161:Mycobacterium / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification; D009171:Mycolic Acids / Q000032:analysis*; D013045:Species Specificity
chemical_list: D009171:Mycolic Acids