High-performance liquid chromatographic determination of vinblastine, 4-O-deacetylvinblastine and the potential metabolite 4-O-deacetylvinblastine-3-oic acid in biological fluids.
Procedures for the determination of vinblastine (VBL), 4-O-deacetylvinblastine (DVBL) and 4-O-deacetylvinblastine-3-oic acid (DVBLA) in biological samples using high-performance liquid chromatography (HPLC) combined with selective sample clean-up are presented. VBL and DVBL were determined in plasma and urine using ion-exchange normal-phase HPLC with fluorescence detection. The limit of detection was 1 microgram/l for both compounds using a 500-microliter sample. Successful chromatographic analyses of DVBLA were achieved by using a glass column packed with 5-microns Hypersil ODS and acetonitrile-0.05 M phosphate buffer (pH 2.7) (23:77, v/v). Positive identification was supported by the use of diode-array detection. The limit of detection (at 270 nm) was 10 micrograms/l using 1-ml samples.
van Tellingen
O
O
Department of Clinical Chemistry, Netherlands Cancer Institute, Amsterdam.
Beijnen
J H
JH
Baurain
R
R
ten Bokkel Huinink
W W
WW
van der Woude
H R
HR
Nooyen
W J
WJ
D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D013050:Spectrometry, Fluorescence; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism* / Q000652:urine
C052416:4-desacetylvinblastine; D014747:Vinblastine; C072973:4-O-deacetylvinblastine-3-oic acid