Cloning and expression of a chitinase gene from Aeromonas hydrophila in Escherichia coli.
An extracellular secreted chitinase gene from Aeromonas hydrophila was cloned in Escherichia coli, and the gene product was detected in the culture medium. Like the natural chitinase protein, the excreted chitinase had a molecular weight of approximately 85,000 and was subject to catabolite repression by glucose.
Chen
J P
JP
Council of Agriculture, Department of Food and Agriculture, Taipei, Republic of China.
Nagayama
F
F
Chang
M C
MC
D000333:Aeromonas / Q000201:enzymology* / Q000235:genetics; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015183:Restriction Mapping
D002688:Chitinases