Induction of the synthesis of the pregnancy-specific protein p70 in the endometrium by intramuscular injection of recombinant bovine interferon-alpha I1 in nonpregnant ewes.
We examined the effect of recombinant bovine interferon-alpha I1 (rboIFN-alpha I1) or recombinant bovine trophoblast protein-1 (rbTP-1) on protein synthesis by endometrial explants from Day-13 cyclic ewes and studied the ability of rboIFN-alpha I1 injected i.m. to influence subsequent protein secretion by endometrial tissue explants. In Expt 1, ewes were injected with either 2 mg rboIFN-alpha I1 or vehicle alone at 12 h intervals beginning on Day 11 of the oestrous cycle and ending on the morning of Day 13; 8 h after the last injection, ewes were hysterectomized and endometrial explant cultures were prepared. Explants were cultured for 24 h in leucine-deficient medium supplemented with 250 microCi L-[3H]leucine per culture. For Expt 2, additional explants were prepared from Expt 1 controls. Explants were cultured in the presence of 0, 20 or 200 ng/ml of either rboIFN-alpha I1 or rbTP-1 for 24 h in leucine-deficient medium supplemented with 250 microCi L-[3H]leucine per culture. Secreted proteins were analysed by two-dimensional electrophoresis and fluorography. There was a marked enhancement of a 70 kDa acidic protein, p70, in explants cultured in the presence of rboIFN-alpha I1 or rbTP-1. This polypeptide is a product of the gravid uterine horn from Day 14 to Day 20 of pregnancy and is a useful marker of the action of interferon-alpha (IFN-alpha) on endometrium. Enhanced production of p70 also occurred in ewes injected i.m. with rboIFN-alpha I1.(ABSTRACT TRUNCATED AT 250 WORDS)
Francis
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Department of Animal Sciences, University of Missouri, Columbia 65211.
Keisler
D H
DH
Roberts
R M
RM
D000818:Animals; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D004717:Endometrium / Q000737:chemistry / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D007273:Injections, Intramuscular; D007370:Interferon Type I / Q000494:pharmacology*; D008186:Luteolytic Agents / Q000037:antagonists & inhibitors; D011257:Pregnancy Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000494:pharmacology; D011994:Recombinant Proteins; D012756:Sheep / Q000378:metabolism*
D007370:Interferon Type I; D008186:Luteolytic Agents; D011257:Pregnancy Proteins; D011994:Recombinant Proteins; C020947:interferon tau; C073044:pregnancy-specific protein p70