title: The development of stimulation-produced analgesia (SPA) in the rat.
abstract: The present study studied the development of stimulation produced analgesia (SPA) from the periaqueductal gray (PAG) in rats. A monopolar stimulating electrode was lowered into the dorsal or ventral PAG of animals aged 7, 14, 21, or 90-120 days. Constant current cathodal pulses (100 Hz, 100 microseconds) were delivered, starting 10 s before analgesia was tested by the tail-flick (TF) test and continuing throughout each TF trial or until cut-off (7 s). Current intensity was increased stepwise (3-200 microA). It was found that SPA can be elicited starting at 21 days, but not earlier. However, supraspinal modulation of nociception is still immature at 3 weeks after birth. First, stimulation intensities needed to produce SPA are higher in 21-day-old pups than in adult animals. Second, in 21-day-old pups, but not in adults effective current intensities in the dorsal PAG are higher than in the ventral PAG. Third, naltrexone decreases SPA from the ventral PAG in 21-day-old pups, but not in adult animals. These findings indicate that supraspinal modulation of nociception develops only 3 weeks after birth, with the ventral PAG maturing prior to the dorsal PAG, and that the contribution of endogenous opioids to SPA does not remain constant throughout the ontogeny of rats.
authors.affiliation: Department of Psychology, Tel-Aviv University, Ramat-Aviv, Israel.
authors.forename: H
authors.initials: H
authors.lastname: van Praag
authors.forename: H
authors.initials: H
authors.lastname: Frenk
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D005260:Female; D009271:Naltrexone / Q000494:pharmacology; D010487:Periaqueductal Gray / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D011930:Reaction Time / Q000187:drug effects; D004561:Transcutaneous Electric Nerve Stimulation
chemical_list: D009271:Naltrexone