Increased immunoreactivity of collagen types I, III and V, fibronectin and TGF-beta in retinal vessels of rats with experimental autoimmune uveoretinitis.
The immunoreactivity of types I, III and V collagen, fibronectin and transforming growth factor-beta (TGF-beta) was studied by an immunogold labeling method in retinal vessels of rats with experimental autoimmune uveoretinitis (EAU) induced by retinal S-antigen. The basal lamina of retinal capillaries in normal rats showed low immunoreactivities for the extracellular matrix components and TGF-beta. However, at the peak of inflammation (day 13-16 postimmunization), labeling of all three types of collagen and fibronectin increased considerably in the basal lamina of retinal vessels. TGF-beta immunoreactivity was detected mainly in the vascular endothelium and the pericytes. The results suggest that TGF-beta synthesized by the endothelial cells and pericytes may regulate the synthesis and composition of extracellular matrix components in the vascular basal lamina during the course of EAU development.
Mahalak
S M
SM
Kresge Eye Institute, Wayne State University School of Medicine, Detroit, MI.
Lin
W L
WL
Essner
E
E
Shichi
H
H
D000818:Animals; D000941:Antigens; D019393:Arrestin; D001324:Autoantigens; D001327:Autoimmune Diseases / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism* / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000378:metabolism / Q000648:ultrastructure; D005136:Eye Proteins; D005260:Female; D005353:Fibronectins / Q000378:metabolism* / Q000648:ultrastructure; D007150:Immunohistochemistry; D008565:Membrane Proteins; D016253:Microscopy, Immunoelectron; D010726:Phosphodiesterase Inhibitors; D051381:Rats; D011917:Rats, Inbred Lew; D012171:Retinal Vessels / Q000378:metabolism* / Q000648:ultrastructure; D012173:Retinitis / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D014605:Uveitis / Q000378:metabolism*
D000941:Antigens; D019393:Arrestin; D001324:Autoantigens; D005136:Eye Proteins; D005353:Fibronectins; D008565:Membrane Proteins; D010726:Phosphodiesterase Inhibitors; D016212:Transforming Growth Factor beta; D003094:Collagen