title: [Long-term follow-up of the accuracy of blood alcohol determination (GC and ADH method) with test sera (about 0.6 and 1.5 g/L) with reference to safety recommendations regarding 0.1 promille].
abstract: For the precision and accuracy testing of the gaschromatographic and the ADH-enzymatic ethanol determination respectively, we used two commercially available control sera (approximately 0.6 g/L and 1.5 g/L respectively). During one year of testing during 1990 at 200 different days, they were daily and routinely used together with the specimen analysed. The mean serum ethanol concentration obtained for both sera was less than the value stat-ed by the manufacturer. Our investigation also aimed at a possible lower threshold value from 0.8 promille to 0.5 promille (BAK) as a consequence of the german "Wiedervereinigung". The combination of GC- with ADH-method always performed the safety-value set up as judged by the german "Bundesgerichtshof" (= 0.1 promille). Even if quality criteria could be hold in this study, some doubt is remaining concerning a sufficient accuracy of blood alcohol measurements in the more complex matrices of individual blood samples to which the results can not be transferred.
authors.affiliation: Institut für Rechtsmedizin, Universität Heidelberg.
authors.forename: R
authors.initials: R
authors.lastname: Aderjan
authors.forename: G
authors.initials: G
authors.lastname: Schmitt
mesh_terms: D000426:Alcohol Dehydrogenase / Q000097:blood*; D000428:Alcohol Drinking / Q000097:blood* / Q000331:legislation & jurisprudence*; D000435:Alcoholic Intoxication / Q000201:enzymology*; D001334:Automobile Driving / Q000331:legislation & jurisprudence*; D002849:Chromatography, Gas; D000431:Ethanol / Q000493:pharmacokinetics*; D006801:Humans; D011446:Prospective Studies; D012016:Reference Values
chemical_list: D000431:Ethanol; D000426:Alcohol Dehydrogenase
vernacular_title: Langzeitkontrolle der Richtigkeit der Blutalkoholbestimmung (GC- und ADH-Methode) mit Testseren (ca. 0.6 bzw. 1.5 g/L) im Hinblick auf Sicherheitszuschläge von 0.1 Promille.