title: [Isolation, purification and immunologic specificity of monoclonal antibodies to antigen p24 of bovine leukosis virus].
abstract: The aim of the work is to develop optimal conditions for identification and purification of monoclonal antibodies specific to protein p24 of bovine leukemia virus (p24-BLV). Two schemes of isolation and purification of monoclonal antibodies are compared. Purified monoclonal antibodies contain two subunits with molecular weight of about 22,000 and 50,000 Da as determined by sodium dodecylsulphate polyacrylamide gel electrophoresis. They react with p24-BLV in the immunoblot assay.
authors.forename: A E
authors.initials: AE
authors.lastname: Mishkinene
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification*; D000918:Antibody Specificity / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D016583:Enzootic Bovine Leukosis / Q000276:immunology*; D016655:HIV Core Protein p24 / Q000276:immunology*; D008970:Molecular Weight
chemical_list: D000911:Antibodies, Monoclonal; D016655:HIV Core Protein p24
vernacular_title: Vydelenie, ochistka i immunologicheskaia spetsifichnost' monoklonal'nykh antitel k antigenu p24 virusa leĭkoza krupnogo rogatogo skota.