Physiological studies on the utilization of oleic acid by Saccharomyces cerevisiae in relation to microbody development.
We studied the influence of specific growth conditions on the induction of beta-oxidation enzymes and rate of microbody proliferation in S. cerevisiae by oleic acid. Of all conditions tested, highest enzyme levels and microbody numbers were achieved in glucose-limited continuous cultures, supplemented with oleic acids second carbon source. Comparable enzyme levels were observed in identical cultures of peroxisome-deficient (pas) mutants of S. cerevisiae. These experiments showed chemostat cultures on glucose/oleic acid mixtures to be a method of choice for future studies on microbody biogenesis/assembly in constructed, conditional pas mutants.
Evers
M E
ME
Laboratory for Electron Microscopy, University of Groningen, Haren, The Netherlands.
Höhfeld
J
J
Kunau
W H
WH
Harder
W
W
Veenhuis
M
M
D002455:Cell Division; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism; D008830:Microbodies / Q000502:physiology* / Q000648:ultrastructure; D009154:Mutation; D019301:Oleic Acid; D009829:Oleic Acids / Q000502:physiology*; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D014170:Transformation, Genetic
D009829:Oleic Acids; D019301:Oleic Acid; D005947:Glucose