Stretch-induced increase of resting intracellular calcium concentration in single guinea-pig ventricular myocytes.
Single guinea-pig ventricular myocytes were loaded with the fluorescent Ca2+ indicator Indo-1 AM and stretched by carbon fibres. Stretching increased resting tension. Sarcomere lengths were increased by 2-18%. It was observed that a stretch increased resting [Ca2+]i in seven out of eight cells. The change in [Ca2+]i increased with the size of the stretch and returned to pre-stretch levels on return to resting cell length. These observations suggest a means by which changes in resting muscle length can modify the contractile state of cardiac muscle.
Le Guennec
J Y
JY
Laboratoire d'Electrophysiologie et de Pharmacologie cellulaires, Université de Tours, Faculté des Sciences, France.
White
E
E
Gannier
F
F
Argibay
J A
JA
Garnier
D
D
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D005453:Fluorescence; D006168:Guinea Pigs; D007211:Indoles / Q000378:metabolism; D009200:Myocardial Contraction; D009206:Myocardium / Q000378:metabolism*; D012518:Sarcomeres / Q000378:metabolism
D007211:Indoles; C059587:Indo-1 pentaacetoxymethyl ester; D002118:Calcium