title: Least-squares estimation of enzyme parameters.
abstract: The estimation of the enzyme parameters Km and Vmax from initial velocity data, or of analogous parameters in binding or transport experiments may be accomplished by transformation of the data, or by a direct weighted least-squares fit. Although the latter makes better use of the data, the method is complex and may be sensitive to initial parameter estimates. We develop a method which reduces the problem to finding the zero of a continuous function of a single variable.
authors.affiliation: School of Medicine, Flinders University, Bedford Park, South Australia.
authors.forename: M E
authors.initials: ME
authors.lastname: Jones
authors.forename: K
authors.initials: K
authors.lastname: Taransky
mesh_terms: D003198:Computer Simulation; D004798:Enzymes / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D016018:Least-Squares Analysis; D008962:Models, Theoretical
chemical_list: D004798:Enzymes