Isolation of the human sex determining region from a Y-enriched yeast artificial chromosome library.
We describe the isolation and characterization of yeast artificial chromosome (YAC) clones spanning the male sex determining region on the short arm of the human Y chromosome. The clones were isolated by hybridizing probes in the interval between the genes MIC2 and ZFY to a Y chromosome-enriched YAC library. The YAC clones were consistent with the order of probes established for this interval and may be useful for functional studies of the region in male sex determination. However, many of the YAC clones from this library carried only one arm of the vector ("half-YACs"), deleted sequences from one end, and contained much smaller inserts (148 kb average) than the size of ligated fragments selected by pulsed-field gel electrophoresis (greater than 440 kb). These problems were overcome by protecting DNA with polyamines during YAC library construction and a second Y-enriched YAC library was constructed with an average insert size of 627 kb.
Monaco
A P
AP
Genome Analysis Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
Müller
U
U
Larin
Z
Z
Meier-Ewert
S
S
Lehrach
H
H
D002460:Cell Line; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D015894:Genome, Human; D006801:Humans; D008297:Male; D012733:Sex Differentiation / Q000235:genetics*; D014998:Y Chromosome