title: Purification and characterization of the N-methylcarbamate hydrolase from Pseudomonas strain CRL-OK.
abstract: A unique cytosolic enzyme that hydrolyzes the carbamate linkage of the insecticide carbaryl (1-naphthyl N-methylcarbamate) was purified from extracts of Pseudomonas sp. strain CRL-OK. Substrates of the hydrolase include the N-methylcarbamate pesticides carbofuran and aldicarb but not the phenylcarbamate isopropyl m-chlorocarbanilate, the thiocarbamate S-ethyl N,N-dipropylthiocarbamate, or the dimethylcarbamate o-nitrophenyldimethylcarbamate.
authors.affiliation: Pesticide Degradation Laboratory, Agricultural Research Service, Beltsville, Maryland 20705.
authors.forename: W W
authors.initials: WW
authors.lastname: Mulbry
authors.forename: R W
authors.initials: RW
authors.lastname: Eaton
mesh_terms: D000581:Amidohydrolases / Q000302:isolation & purification / Q000378:metabolism*; D002219:Carbamates / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D015394:Molecular Structure; D011549:Pseudomonas / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D002219:Carbamates; C020401:N-methylcarbamate; D000581:Amidohydrolases; C072784:N-methylcarbamate hydrolase