Characterization of murine monoclonal antibodies against serogroup B salmonellae and application as serotyping reagents.
Six murine hybridoma monoclonal antibodies reactive with lipopolysaccharide antigens of Salmonella typhimurium were obtained from a fusion of immune spleen cells from mice immunized with S. typhimurium and NS1 myeloma cells. Four antibodies appeared to be specific for serogroup B salmonellae, while the remaining two antibodies were found to be cross-reactive with Salmonella paratyphi A. The exquisite specificities of the Salmonella serogroup B monoclonal antibodies were demonstrated by their unique reactivities with different serotypes of group B salmonellae but with neither other O serogroups of salmonellae nor a wide spectrum of standard strains of other bacterial species. Serotyping of salmonella strains by the slide agglutination method with two of the serogroup B-specific monoclonal antibodies demonstrated their usefulness as serotyping reagents for the identification of serogroup B salmonellae in a routine diagnostic bacteriology laboratory.
Tsang
R S
RS
Department of Microbiology, University of Hong Kong, Queen Mary Hospital Compound.
Chan
K H
KH
Lau
N W
NW
Choi
D K
DK
Law
D K
DK
Ng
M H
MH
D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D005069:Evaluation Studies as Topic; D006825:Hybridomas / Q000276:immunology; D007120:Immunochemistry; D007202:Indicators and Reagents; D051379:Mice; D012475:Salmonella / Q000145:classification / Q000276:immunology*; D012703:Serotyping / Q000379:methods
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents