title: Structural analysis of the gene encoding rat uricase.
abstract: The uricase gene was isolated from rat genomic DNA libraries. The gene spans 40 kb and consists of eight exons. All the exon-intron junctional sequences conform to the canonical GT/AG rule. The restriction map of the isolated clones and Southern blot analysis revealed that the enzyme is encoded by a single-copy gene. Analysis of the transcription initiation site of rat uricase mRNA indicated the differential use of consecutive nucleotides; the principal repeat is located 55 nucleotides upstream from the first methionine codon. Nucleotide sequence analysis of the 5'-flanking region showed the presence of a TATA (ATAAAA) sequence at nucleotides 30 to 25 and of a CAAT (GGTCAAT) sequence at nucleotides 63 to 57 upstream of the principal transcription initiation site. The 5'-flanking region contains another possible regulatory sequence (TGTCGACA) homologous to the cAMP-regulatory element. The palindromic sequence is located 158 to 151 nucleotides upstream from the transcription initiation site, surrounded by a direct repeat (TCAGCAA).
authors.affiliation: Division of Molecular Genetics, School of Medicine, Fujita Health University, Aichi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Ogawa
authors.forename: S
authors.initials: S
authors.lastname: Kato
authors.forename: Y
authors.initials: Y
authors.lastname: Takagi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003360:Cosmids; D004247:DNA; D005091:Exons; D005796:Genes; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D012326:RNA Splicing; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping; D016415:Sequence Alignment; D014158:Transcription, Genetic; D014503:Urate Oxidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D004247:DNA; D014503:Urate Oxidase