Automated monitoring system for determining embryo movement and death times following virus infection.
Design and performance information is presented on an automated embryo-monitoring system for intact eggs. The computer-based system has been used successfully for several months to characterize viral pathogenicity in embryonated eggs. Features include electronic sensing of embryo movement, automatic quantification of the amount of movement, and automatic recording of the results on electronic media. The system does not require that eggs be removed from the incubator or that the incubator be opened during the course of an experiment, as is necessary with the manual candling technique. It has greatly improved discrimination of viral pathogenicity effects in fertile eggs because of its sensitivity and reduced intervals between observations. One important potential application involves using the system to measure the effects on virulence of mixing closely related variants of the same strain of virus that differ in pathogenicity, which is the biological scenario most likely approximating a natural disease outbreak.
Mitchell
B W
BW
U.S. Department of Agriculture, Southeast Poultry Research Laboratory, Athens, Georgia 30605.
Perdue
M L
ML
D000818:Animals; D002642:Chick Embryo / Q000382:microbiology* / Q000502:physiology; D009980:Influenza A virus / Q000472:pathogenicity*; D007928:Lethal Dose 50; D008838:Microcomputers; D009068:Movement; D012984:Software