title: Isoenzymes of pyruvate kinase, lactate dehydrogenase and alkaline phosphatase in epithelial cell lines of rat liver.
abstract: In cultured epithelial cells of rat liver the isoenzyme patterns of pyruvate kinase, lactate dehydrogenase and alkaline phosphatase were studied and compared with those of freshly isolated parenchymal and non-parenchymal liver cells. In all epithelial cell lines pyruvate kinase was not activated by fructose 1,6-bisphosphate, suggesting the absence of the L-isoenzyme. Cell lines derived from livers of newborn rats expressed LDH-4 and -5, whereas cell lines developed from fetal rat livers contained all 5 lactate dehydrogenase isoenzymes. In the latter case the pattern was found to depend on the state of confluence. All cell lines exhibited only a single alkaline phosphatase form, however, differences were found with respect to electrophoretic mobility.
authors.affiliation: University of Leipzig, School of Medicine, Institute of Biochemistry, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Kässner
authors.forename: G
authors.initials: G
authors.lastname: Neupert
authors.forename: R
authors.initials: R
authors.lastname: Scheibe
authors.forename: K W
authors.initials: KW
authors.lastname: Wenzel
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D011770:Pyruvate Kinase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D011770:Pyruvate Kinase; D000469:Alkaline Phosphatase