Plasmid-encoded determinants for bacteriocin production and immunity in a Lactococcus lactis strain and purification of the inhibitory peptide.
Lactococcin, a bacteriocin produced by Lactococcus lactis subsp. lactis ADRIA 85LO30, was purified as a 2.3-2.4 kDa peptide. Six non-bacteriocin-producing (Bac-) and non-immune (Imm-) strains were isolated after curing experiments. These strains had in common the loss or modification of two plasmids: pOS4 (32 kb) and pOS5 (70 kb). By comparing pOS5 and several modified plasmids, a DNA region from pOS5 of about 10 kb, which was necessary for wild-type bacteriocin production and immunity, was identified.
Dufour
A
A
URA 256, Laboratoire de Génétique Moléculaire, Université de Rennes, France.
Thuault
D
D
Boulliou
A
A
Bourgeois
C M
CM
Le Pennec
J P
JP
D001430:Bacteriocins / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D005798:Genes, Bacterial; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010957:Plasmids
D001430:Bacteriocins; D004269:DNA, Bacterial; D004274:DNA, Recombinant; C069325:lactococcin A