Surface glycoconjugates of cynomolgus monkey trabecular cells.
Non-ionic detergent-soluble and detergent-resistant cynomolgus monkey trabecular cell surface glycoconjugates have been identified using three radiolabeling procedures and polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The cell surface glycoconjugates were radiolabeled with tritiated sodium borohydride after treatment with: (1) neuraminidase/galactose oxidase; (2) galactose oxidase; or (3) sodium metaperiodate. The non-ionic detergent soluble components were removed by treatment of the monolayer cell culture with 1% Triton X-100 in hypotonic buffer. The radiolabeled trabecular cell surface glycoconjugates in the Triton X-100 soluble and insoluble fractions were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis under reducing conditions and visualized by fluorography. Treatment of the monkey trabecular cells with neuraminidase/galactose oxidase/tritiated sodium borohydride resulted in the radiolabeling of five components ranging in apparent molecular weight from 128,000 to 39,000 in the Triton X-100 soluble fractions, while 12 radiolabeled glycoconjugates with apparent molecular weights ranging from 240,000 to 26,000 were resolved in the Triton X-100 resistant fraction.
Kress
B C
BC
Department of Neurosciences, University of California, San Diego, La Jolla 92093.
Weinreb
R N
RN
Pinney
E
E
Miller
A L
AL
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D005692:Galactose Oxidase; D006001:Glycoconjugates / Q000032:analysis*; D007553:Isotope Labeling; D008252:Macaca fascicularis; D008858:Microscopy, Phase-Contrast; D009439:Neuraminidase; D017830:Octoxynol; D011092:Polyethylene Glycols; D011868:Radioisotopes; D014129:Trabecular Meshwork / Q000737:chemistry* / Q000166:cytology
D003902:Detergents; D006001:Glycoconjugates; D011868:Radioisotopes; D011092:Polyethylene Glycols; D017830:Octoxynol; D005692:Galactose Oxidase; D009439:Neuraminidase