[Comparative analysis of the effects of the unpurified preparations of murine erythropoietin and human recombinant erythropoietin on erythroid and granulocyte-macrophage progenitor cells in semi-solid mouse bone marrow cultures].
Effects of unpurified murine erythropoietin and unpurified human recombinant erythropoietin on the growth of erythroid--BFU-E and granulocyte--macrophage progenitor cells--CFU--GM from the mouse bone marrow were compared using a methylcellulose culture system. Average erythropoietin titers for murine serum and supernatant human recombinant erythropoietin were 16 U/ml and 33 U/ml, respectively. The maximal stimulation was observed at 1-2 U/ml culture recombinant erythropoietin and 0.5 U/ml culture murine erythropoietin. Murine erythropoietin was more effective then human one. Murine and human recombinant erythropoietin had no significant effect on the number of CFU-GM colonies. But human recombinant erythropoietin could be preferentially used when studying the mechanism of erythropoiesis in man and animals because there were erythropoiesis inhibitors in mouse serum.
Manakova
T E
TE
Kapina
M A
MA
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D003114:Colony-Forming Units Assay; D015672:Erythroid Precursor Cells / Q000187:drug effects*; D004920:Erythropoiesis; D004921:Erythropoietin / Q000494:pharmacology*; D005260:Female; D006098:Granulocytes / Q000187:drug effects; D006801:Humans; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009924:Organ Culture Techniques
D004921:Erythropoietin
Sravnitel'nyĭ analiz deĭstviia neochishchennykh preparatov myshinogo éritropoétina i rekombinantnogo éritropoétina cheloveka na éritroidnye i granulotsitarno-makrofagal'nye kletki-predshestvenniki vpolutverdykh kul'turakh kostnogo mozga mysheĭ.