Cuticular proteins from the shrimp, Pandalus borealis.
1. The percentages of mineral salts, chitin, and urea-extractable and non-extractable proteins were determined in pieces of cuticle from selected body regions of the shrimp, Pandalus borealis. 2. Two-dimensional gel-electrophoresis of the urea-extractable proteins shows that a large number of different proteins are present. Identical protein patterns are obtained from the various cuticular regions. 3. A fractionation scheme is presented, which is suitable for obtaining the major proteins in quantities sufficient for further characterization. The amino acid compositions are reported for several of the proteins.
Andersen
S O
SO
Institute of Biological Chemistry A, August Krogh Institute, Copenhagen, Denmark.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D015180:Electrophoresis, Gel, Two-Dimensional; D009928:Organ Specificity; D033542:Pandalidae / Q000737:chemistry*; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification
D000596:Amino Acids; D011506:Proteins