Fluorometric determination of phosphatidylcholine as a measure of phospholipid methylation.
The successive methylation of phosphatidylethanolamine to phosphatidylcholine (phospholipid methylation) has been measured by the incorporation of S-[methyl-3H]adenosylmethionine or colorimetric assay of phosphatidylcholine extracted from adipocyte plasma membranes. A fluorometric assay for phosphatidylcholine was developed to measure phospholipid methylation. This assay is 10 times more sensitive than the colorimetric assay and demonstrates no significant interference with other methylated phospholipids. The fluorometric assay was used to determine a biphasic insulin dose response in adipocyte plasma membranes. This fluorometric assay for phosphatidylcholine represents an alternative method for monitoring phospholipid methylation, especially when increased sensitivity is required.
Dandurand
D M
DM
Department of Clinical Pathology, William Beaumont Hospital, Royal Oak, Michigan 48073.
Kiechle
F L
FL
Strandbergh
D R
DR
Zak
B
B
Artiss
J D
JD
D000273:Adipose Tissue / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D003124:Colorimetry / Q000379:methods; D005470:Fluorometry / Q000379:methods; D008297:Male; D008745:Methylation; D010713:Phosphatidylcholines / Q000032:analysis*; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D010713:Phosphatidylcholines; D010743:Phospholipids