title: Assessment of two commercial agglutination kits for detecting Escherichia coli heat-labile enterotoxin.
abstract: Two commercial agglutination kits, a reserved passive agglutination test (VET-RPLA) and a staphylococcal coagglutination test (Phadebact ETEC-LT Test), were compared with two cell culture assays (Y-1 and Vero) and GM1 ganglioside enzyme-linked immunosorbent assay (GM1-ELISA) for sensitivity in detecting Escherichia coli heat-labile enterotoxin (LT). Of 48 toxigenic strains, 23 were positive by all assays. One strain was negative only by the Phadebact test. Four strains, all LT-II producers, were positive by cell culture only. For LT-I detection, the Phadebact test was the least sensitive but was simple and rapid; VET-RPLA was simple, sensitive, and a good substitute for cell culture or GM1-ELISA.
authors.affiliation: Bureau of Microbial Hazards, Health and Welfare Canada, Ottawa, Ont.
authors.forename: J
authors.initials: J
authors.lastname: Speirs
authors.forename: S
authors.initials: S
authors.lastname: Stavric
authors.forename: B
authors.initials: B
authors.lastname: Buchanan
mesh_terms: D000372:Agglutination Tests / Q000379:methods*; D001427:Bacterial Toxins / Q000032:analysis*; D004768:Enterotoxins / Q000032:analysis*; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins; D005069:Evaluation Studies as Topic; D011933:Reagent Kits, Diagnostic
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D011933:Reagent Kits, Diagnostic; C027538:heat-labile enterotoxin, E coli