title: A spectrophotometric assay for lipase activity utilizing immobilized triacylglycerols.
abstract: New substrates for the determination of lipase activity have been developed. Triacylglycerols were immobilized by adsorption on an appropriate carrier or adsorbent yielding a lipase substrate in a powder form. The adsorbed triacylglycerols were easily hydrolyzed by lipases present in a reaction mixture. The released fatty acids were extracted with benzene and converted to the corresponding Cu (II) salts (copper soaps) which were measured spectrophotometrically.
authors.affiliation: Laboratory of Analytical Chemistry, South Bohemian Biological Centre, Branisovská, Ceské Budĕjovice, Czechoslovakia.
authors.forename: I
authors.initials: I
authors.lastname: Safarík
mesh_terms: D000327:Adsorption; D002175:Candida / Q000201:enzymology; D008049:Lipase / Q000378:metabolism*; D013053:Spectrophotometry; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D014280:Triglycerides; D008049:Lipase