Chromosome analysis of multipronuclear human oocytes after in vitro fertilization.
Multipronuclear human eggs are frequent after in vitro fertilization. Their chromosome analysis can provide useful information. Before cleavage it can confirm the suspected polyploidy. Among the cleaved multipronuclear eggs it provides an estimation of the incidence of the possible return to diploidy. Ninety-four multipronuclear eggs were fixed at the first, second, or third cleavage according to the air-drying method of Tarkowski with or without colchicine exposure: 60 were successfully analysed. Twelve were stopped before cleavage (six without colchicine treatment and six with colchicine treatment). They were polyploid, confirming the cytological observation. Forty-eight eggs cleaved and were stopped by colchicine treatment and karyotyped. Seventeen eggs (35 per cent) had produced diploid embryos. Mosaicism was frequent (15 cases, 31 per cent). Triploidy was not frequent (8 eggs, 17 per cent). Haploidy constituted the remaining cases (8 eggs, 17 per cent). Our data indicate that the initial count of pronuclei is a reliable test. Multipronuclear one-cell oocytes were confirmed to be polyploid. Furthermore, the developmental capacity of the multipronuclear oocytes is variable. Most of them cleaved. However, many multipronuclear oocytes led to diploid cleaving eggs.
Martin-Pont
B
B
Laboratoire d'Histologie Embryologie Cytogénétique, CHU Bobigny, France.
Selva
J
J
Bergere
M
M
Fillion
C
C
Hugues
J N
JN
Tamboise
A
A
Tamboise
E
E
D000328:Adult; D002970:Cleavage Stage, Ovum / Q000648:ultrastructure; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007621:Karyotyping; D009030:Mosaicism; D009865:Oocytes / Q000648:ultrastructure; D011123:Polyploidy