title: Preparation and storage of "leukocyte-depleted single donor" platelet concentrate: a step forward in effective haemotherapy.
abstract: "Leukocyte-depleted" platelet concentrates (LD-SDPC) were prepared by cotton-wool and by polyester filtration of "leukocyte-poor" PC collected by the elutriation technique. The storage characteristics of LD-SDPC were comparable to those of the filtered pools of multiple donor platelet concentrates (LD-MDPC). However, LD-SDPC were less activated and less damaged than LD-MDPC over a 7-day storage period, as evidenced by beta-thromboglobin percent release and lactic dehydrogenase percent leakage in both products. LD-SDPC were prophylactically transfused to 12 thrombocytopenic patients; the mean bleeding time was shortened from 12 min 20 sec to 4 min 39 sec. Corrected count increment (CCI) was 22.8 at 1 hr and 14.1 at 24 hr compared to 15.5 and 10.0, respectively, with standard PCs.
authors.affiliation: Red Cross Blood Bank Groningen-Drenthe, The Netherlands.
authors.forename: M K
authors.initials: MK
authors.lastname: Elias
authors.forename: J T
authors.initials: JT
authors.lastname: de Wolf
authors.forename: N
authors.initials: N
authors.lastname: Blom
authors.forename: L
authors.initials: L
authors.lastname: Rijskamp
authors.forename: R M
authors.initials: RM
authors.lastname: Halie
authors.forename: C T
authors.initials: CT
authors.lastname: Smit Sibinga
mesh_terms: D016913:Blood Component Transfusion; D001792:Blood Platelets / Q000648:ultrastructure; D001793:Blood Preservation / Q000379:methods*; D002470:Cell Survival; D005374:Filtration; D003368:Gossypium; D006487:Hemostasis; D006801:Humans; D008212:Lymphocyte Depletion; D010974:Platelet Aggregation; D010976:Platelet Count; D017713:Platelet Transfusion; D010983:Plateletpheresis / Q000295:instrumentation; D011091:Polyesters; D013921:Thrombocytopenia / Q000628:therapy
chemical_list: D011091:Polyesters