Hierarchical regulation of interleukin production: induction of interleukin 6 (IL-6) production from bone marrow cells and marrow stromal cells by interleukin 3 (IL-3).
IL-3 stimulated the production of IL-6 from a bone marrow-adherent cell population, macrophages and from hemopoietic supportive stromal cell lines. It also induced IL-6 production from a stem cell-enriched population of bone marrow cells which did not produce IL-6 without stimulation. In contrast, stimulation with IL-6 of all the cell populations studied in the present experiments did not induce IL-3 production. These results indicate a hierarchical network in the regulation of interleukin production, and existence of a positive feedback mechanism; IL-3 induces IL-6 production which in turn stimulates stem cells into cycle and induces stem cells to respond to IL-3.
Miyashita
M
M
Department of Biology, Faculty of Science, Niigata University, Japan.
Sugimoto
K
K
Suzuki
J
J
Taniguchi
S
S
Aramaki
K
K
Mori
K J
KJ
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D005246:Feedback; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D007377:Interleukin-3 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000096:biosynthesis*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H
D007377:Interleukin-3; D015850:Interleukin-6