Black substrate for spectrophotometric determination of cellulase activity in coloured solutions.
A simple procedure for spectrophotometric determination of cellulase activity in coloured solutions is described. CM-cellulose, cross-linked with epichlorhydrin in the presence of black drawing ink, is used as an insoluble chromolytic substrate. The absorbance of reaction mixture filtrates are read in the near infra-red region (at 800-900 nm) where the absorbances of contaminating coloured substances are substantially lowered; by contrast, black drawing ink released from the substrate during the action of cellulases absorbs strongly at these wavelengths.
Safarík
I
I
Laboratory of Analytical Chemistry, South Bohemian Biological Centre, Ceské Budĕjovice, Czechoslavakia.
Safaríková
M
M
D002266:Carboxymethylcellulose Sodium / Q000737:chemistry; D002480:Cellulase / Q000032:analysis*; D003432:Cross-Linking Reagents; D004811:Epichlorohydrin; D005374:Filtration; D006868:Hydrolysis; D007281:Ink; D007700:Kinetics; D012995:Solubility; D012996:Solutions; D013053:Spectrophotometry / Q000379:methods; D013379:Substrate Specificity
D003432:Cross-Linking Reagents; D012996:Solutions; D004811:Epichlorohydrin; D002480:Cellulase; D002266:Carboxymethylcellulose Sodium