Depletion of glutathione interferes with induction of glycerolphosphate dehydrogenase in the brains of young rats.
In the rat brain hydrocortisone induces the enzyme, glycerolphosphate dehydrogenase (GPDH), during the first postnatal week. The present studies focused on a hypothetical role for glutathione-S-transferase (GST) in that phenomenon. Two forms of GST, Yb and Yp, had been detected in glial cells in mature rat brains, and it was suggested that they might function in hormone transport. Now GSTs have also been observed in the brains of 1-day-old rats. Two glutathione-depleting agents, buthionine sulfoximine and cyclohexene-1-one, were administered to rats, along with hydrocortisone, during the first postnatal week. Hydrocortisone or a depleting agent alone was administered to control animals. During the early days of the experiment there were lower GPDH specific activities in brains from the animals given hydrocortisone plus a depleting agent than in those from animals given hydrocortisone alone. Depleting agents alone did not affect the specific activities of GPDH. It is suggested that one function of the GST in rat brain is transport of hydrocortisone between or within glial cells.
Tansey
F A
FA
Department of Neurology, Albert Einstein College of Medicine, Bronx, NY 10461.
Cammer
W
W
D000375:Aging / Q000378:metabolism; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D001921:Brain / Q000201:enzymology*; D019328:Buthionine Sulfoximine; D003512:Cyclohexanones / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005978:Glutathione / Q000502:physiology*; D005982:Glutathione Transferase / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000096:biosynthesis*; D006854:Hydrocortisone / Q000494:pharmacology; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism; D008298:Maleates / Q000494:pharmacology; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D009457:Neuroglia / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
D000963:Antimetabolites; D003512:Cyclohexanones; D007527:Isoenzymes; D008298:Maleates; D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; C036468:cyclohexanone; D005993:Glycerolphosphate Dehydrogenase; D005982:Glutathione Transferase; C014476:diethyl maleate; D005978:Glutathione; D006854:Hydrocortisone