Translation of double-stranded RNA segments 1 and 2 of Chuzan virus in rabbit reticulocyte lysates.
Coding assignments of genome segments 1 and 2 of Chuzan virus strain K-47 were studied in vitro by using rabbit reticulocyte lysates. The double-stranded RNA segment was extracted from sodium dodecyl sulfate (SDS)-polyacrylamide gels by a modified crushing and eluting technique. Translation products labeled with [35S]methionine were resolved by SDS-polyacrylamide gel electrophoresis. The molecular weights of the products from RNA segments 1 and 2 were estimated to be 98 and 95 kilodaltons, respectively.
Yoshida
T
T
Department of Veterinary Microbiology, Faculty of Agriculture, Miyazaki University, Japan.
Harasawa
R
R
Goto
Y
Y
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000032:analysis* / Q000737:chemistry; D011817:Rabbits; D012087:Reoviridae / Q000235:genetics*; D012156:Reticulocytes / Q000382:microbiology*
D012367:RNA, Viral