Purification, properties, and sequence specificity of SslI, a new type II restriction endonuclease from Streptococcus salivarius subsp. thermophilus.
SslI, a type II restriction endonuclease, was purified from Streptococcus salivarius subsp. thermophilus strain BSN 45. SslI is an isoschizomer of BstNI. SslI activity was maximum at pH 8.8, 0 to 50 mM NaCl, 2 to 8 mM Mg2+, and 42 degrees C. Activity against phage DNA in vitro was demonstrated.
Benbadis
L
L
Centre International de Recherche Daniel Carasso, Le Plessis Robinson, France.
Garel
J R
JR
Hartley
D L
DL
D002118:Calcium / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D015252:Deoxyribonucleases, Type II Site-Specific / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D009994:Osmolar Concentration; D013291:Streptococcus / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature
D008345:Manganese; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D008274:Magnesium; D002118:Calcium