Phthalocyanine-induced photodynamic changes of cytoplasmic free calcium in Chinese hamster cells.
Exposure to light of Chinese hamster cells preloaded with chloroaluminum phthalocyanine causes an immediate increase of cytoplasmic free calcium, [Ca2+], from about 0.2 microM to 1 microM within 5 min after illumination. This increase was dose-dependent within the biological dose range, reaching a plateau at a dose that kills 99.5% of the cells. Fluoride addition prior to light exposure protected against cell killing and reduced the increase of [Ca2+]i. These findings raise the possibility that changes in [Ca2+]i after photodynamic treatment may be relevant to cell killing and/or other biological responses of the cells, e.g. release of eicosanoids.
Ben-Hur
E
E
Sylvius Laboratory, Dept. of Medical Biochemistry, Leiden, The Netherlands.
Dubbelman
T M
TM
Van Steveninck
J
J
D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006224:Cricetinae; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D007211:Indoles / Q000494:pharmacology*; D008027:Light; D009942:Organometallic Compounds / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*
D007211:Indoles; D009942:Organometallic Compounds; D011838:Radiation-Sensitizing Agents; C044797:chloroaluminum phthalocyanine; D000535:Aluminum; D002118:Calcium