Hyperpolarization and intracellular acidification in Trichoderma viride as a response to illumination.
Using indirect methods based on uptake of [3H]tetraphenylphosphonium cation and [14C]benzoic acid by cells of the fungus Trichoderma viride we found that the illumination-induced transient hyperpolarization of the plasma membrane is followed immediately by a rapid temporary decrease in intracellular pH. Hyperpolarization and intracellular acidification were completely suppressed by 150 mM-KCl and by the K(+)-ionophore valinomycin. The light-induced acidification of the cytoplasm was not observed in the presence of the cytochrome respiratory chain inhibitors antimycin A and mucidin. Based on these results, we hypothesize that the hyperpolarization of the cells is the consequence of an efflux of K+ through a light-activated K(+)-channel in the plasma membrane. The loss of positive charge in the cytoplasm caused by this efflux of cations is counterbalanced by H+ originating from the light-activated mitochondrial respiratory chain.
Gresík
M
M
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Kolarova
N
N
Farkas
V
V
D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008027:Light; D008564:Membrane Potentials / Q000528:radiation effects; D011188:Potassium / Q000378:metabolism; D014242:Trichoderma / Q000378:metabolism / Q000528:radiation effects*
D011188:Potassium