title: Cloning and nucleotide sequencing of the gene encoding the beta-lactamase from Citrobacter diversus.
abstract: The gene coding for the class A beta-lactamase of Citrobacter diversus has been cloned and sequenced. It contains the information for a 294-amino-acid precursor protein, including a 27-residue N-terminal signal peptide. The deduced sequence of the N-terminal portion of the mature protein is in excellent agreement with that determined by microsequencing of the protein and readily explains the pI differences observed between the naturally occurring forms I and II of the enzyme. The sequence of the mature protein exhibits a very high degree of similarity with that of the Klebsiella oxytoca class A beta-lactamase.
authors.affiliation: Department of Biomedical Sciences and Technology and Biometry, Località Collemaggio, L'Aquila, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Perilli
authors.forename: N
authors.initials: N
authors.lastname: Franceschini
authors.forename: B
authors.initials: B
authors.lastname: Segatore
authors.forename: G
authors.initials: G
authors.lastname: Amicosante
authors.forename: A
authors.initials: A
authors.lastname: Oratore
authors.forename: C
authors.initials: C
authors.lastname: Duez
authors.forename: B
authors.initials: B
authors.lastname: Joris
authors.forename: J M
authors.initials: JM
authors.lastname: Frère
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002954:Citrobacter / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D012689:Sequence Homology, Nucleic Acid; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D009838:Oligodeoxyribonucleotides; D001618:beta-Lactamases