Inhibitory effects of Maharishi-4 and Maharishi-5 on microsomal lipid peroxidation.
The effects of Maharishi-4 (M-4) and Maharishi-5 (M-5) on microsomal lipid peroxidation were examined in vitro. Rat liver microsomes were incubated with an NADPH-generating system or with sodium ascorbate and an ADP-iron complex to stimulate enzymatic or nonenzymatic lipid peroxidation respectively. Alcoholic or aqueous extracts of M-4 or M-5, when added to these incubation systems, inhibited hepatic microsomal lipid peroxidation in a concentration-dependent manner. The aqueous extract of M-4 was the most effective antiperoxidant in these systems. A 10% (w/v) aqueous extract of M-4 inhibited ascorbate or NADPH-induced lipid peroxidation by approximately 50% when added at volumes of 8 microliters and 3.5 microliters respectively to the incubation mixtures (total incubation volume, 2 ml). These findings suggest that M-4 and M-5, by virtue of their antiperoxidant properties, may be useful in the treatment of free radical-linked drug toxicities and disease states.
Dwivedi
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C
College of Pharmacy, South Dakota State University, Brookings 57007.
Sharma
H M
HM
Dobrowski
S
S
Engineer
F N
FN
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005527:Food, Fortified; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008515:Medicine, Ayurvedic; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000975:Antioxidants; D008315:Malondialdehyde