Histological and functional changes in the thyroid glands of mice implanted with hybridomas secreting monoclonal autoantibody against mouse thyroglobulin.
Mouse hybridoma cells secreting monoclonal antibody (mAb) against mouse thyroglobulin were established. The implantation of the hybridomas succeeded to induce high titers of circulating mAb against thyroglobulin in sera of mice. By using the implantation of the hybridomas in mice, the effect of autoantibody on the thyroid glands was studied histologically and functionally. In these mice the thyroid follicles were significantly swollen and warped, whereas there was no infiltration of inflammatory cells. The 125I-uptake in their thyroid glands was markedly decreased. There were no functional changes in control mice implanted with non-secreting P3U1 partner cells. Therefore, it was suggested that high titers of anti-thyroglobulin autoantibody could definitely cause the histological and functional damages in the thyroid glands.
Yokochi
T
T
Department of Microbiology, Aichi Medical University School of Medicine, Japan.
Inoue
Y
Y
Fukada
M
M
Kawai
M
M
Yoshikawa
K
K
Suzuki
Y
Y
Kato
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000633:toxicity*; D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology / Q000633:toxicity*; D006825:Hybridomas / Q000276:immunology / Q000637:transplantation*; D007274:Injections, Intraperitoneal; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D013954:Thyroglobulin / Q000276:immunology*; D013961:Thyroid Gland / Q000473:pathology; D013967:Thyroiditis, Autoimmune / Q000209:etiology* / Q000276:immunology / Q000378:metabolism / Q000473:pathology
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D007457:Iodine Radioisotopes; D013954:Thyroglobulin