title: [The effects of calcium concentration of reperfusion solution upon myocardial protection].
abstract: The effects of several calcium concentrations in reperfusion solution were studied. Experimental time course was as followed: 20 min working perfusion, 3 min cardioplegic infusion with St. Thomas cardioplegic solution (STS) followed by global ischemia for 30 min at 37.5 degrees C, 15 min early Langendorff reperfusion with reperfusion solution and 5 min late reperfusion with Krebs Henseleit bicarbonate buffer [( Calcium] = 2.5 mM), followed by 20 min working perfusion. Percent recoveries of aortic flow at the Ca concentration of 0, 0.1, 0.6, 1.2, 1.8, 2.5 mM were 0, 14 +/- 1, 43 +/- 4, 64 +/- 3, 55 +/- 2, 59 +/- 1 (%), respectively. Our data indicated that reperfusion solution with less than 1.2 mM calcium reduced the protective properties of STS.
authors.affiliation: Department of Cardiovascular Surgery, National Cardiovascular Center, Oska, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohashi
authors.forename: F
authors.initials: F
authors.lastname: Yamamoto
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: H
authors.initials: H
authors.lastname: Ichikawa
authors.forename: H
authors.initials: H
authors.lastname: Komai
authors.forename: T
authors.initials: T
authors.lastname: Shibata
authors.forename: N
authors.initials: N
authors.lastname: Nakajima
authors.forename: Y
authors.initials: Y
authors.lastname: Kawashima
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis / Q000494:pharmacology*; D002314:Cardioplegic Solutions / Q000032:analysis / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D006324:Heart Arrest, Induced; D066298:In Vitro Techniques; D008297:Male; D015425:Myocardial Reperfusion; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002314:Cardioplegic Solutions; D002118:Calcium