Evolution of the dec-1 eggshell locus in Drosophila. I. Restriction site mapping and limited sequence comparison in the melanogaster species subgroup.
We have analyzed approximately 18 kb of DNA in and upstream of the defective chorion-1 (dec-1) locus of the eight known species of the melanogaster species subgroup of Drosophila. The restriction maps of D. simulans, D. mauritiana, D. sechellia, D. erecta, and D. orena are shown to have basically the restriction map of D. melanogaster, whereas the maps of D. teissieri and D. yakuba were more difficult to align. However, the basic amount of DNA and sequence arrangement appear to have been conserved in these species. A small deletion of varying length (65-200 bp) is found in a repeated sequence of the central transcribed region of D. melanogaster, D. simulans, and D. erecta. Restriction site mapping indicated that the dec-1 gene is highly conserved in the melanogaster species subgroup. However, sequence comparison revealed that the amount of nucleotide and amino acid substitution in the repeated region is much larger than in the 5' translated region. The 5' flanking region showed noticeable restriction site polymorphisms between species. Based on calculations from the restriction maps a dendrogram was derived that supports earlier published phylogenetic relationships within the melanogaster species subgroup except that the erecta-orena pair is placed closer to the melanogaster complex than to D. teissieri and D. yakuba.
Andersson
S
S
Department of Genetics, University of Umeå, Sweden.
Lambertsson
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002823:Chorion / Q000378:metabolism*; D004247:DNA; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000145:classification / Q000235:genetics*; D004527:Egg Proteins / Q000235:genetics*; D014644:Genetic Variation; D007301:Insect Hormones / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D010802:Phylogeny; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D029721:Drosophila Proteins; D004527:Egg Proteins; D007301:Insect Hormones; C066329:dec-1 protein, Drosophila; D004247:DNA