title: DNA of some anaerobic rumen fungi: G + C content determination.
abstract: The nuclear DNAs from five species of anaerobic rumen fungi have been isolated and purified by means of two extraction methods (with and without 8 M urea). Their G + C contents have been characterized by the thermal denaturation procedure of Marmur and Doty. As has already been shown in Neocallimastix frontalis, the results obtained by the two techniques demonstrated a very low G + C content (less than 20%) and the constant presence of satellite DNA.
authors.affiliation: ICBMC, Section Levures, Université Lyon 1, Villeurbanne, France.
authors.forename: G
authors.initials: G
authors.lastname: Billon-Grand
authors.forename: J B
authors.initials: JB
authors.lastname: Fiol
authors.forename: A
authors.initials: A
authors.lastname: Breton
authors.forename: A
authors.initials: A
authors.lastname: Bruyère
authors.forename: Z
authors.initials: Z
authors.lastname: Oulhaj
mesh_terms: D000818:Animals; D001482:Base Composition; D002417:Cattle; D004271:DNA, Fungal / Q000032:analysis*; D005658:Fungi / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D012417:Rumen / Q000382:microbiology*; D012418:Ruminants / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal