title: Estimation of tyrosine by the attenuation of 4-iodophenol enhanced chemiluminescence.
abstract: Tyrosine markedly attenuates the chemiluminescence output intensity from the 4-iodophenol enhanced chemiluminescence assay system in a manner consistent with competition between the amino acid and luminol for the 4-iodophenoxy radical. This effect provides the basis for a sensitive assay of tyrosine. Interference by the other amino acids has been assessed; major interference by cysteine can be removed by incubation with iodoacetic acid.
authors.affiliation: Department of Chemistry, University of Newcastle upon Tyne, UK.
authors.forename: T E
authors.initials: TE
authors.lastname: Candy
authors.forename: D
authors.initials: D
authors.lastname: Mantle
authors.forename: P
authors.initials: P
authors.lastname: Jones
mesh_terms: D002729:Chloromercuribenzoates; D007461:Iodoacetates; D019807:Iodoacetic Acid; D007462:Iodobenzenes / Q000737:chemistry / Q000494:pharmacology*; D008163:Luminescent Measurements; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds; D014443:Tyrosine / Q000032:analysis*; D020245:p-Chloromercuribenzoic Acid
chemical_list: D002729:Chloromercuribenzoates; D007461:Iodoacetates; D007462:Iodobenzenes; D013438:Sulfhydryl Compounds; D014443:Tyrosine; C050181:4-iodophenol; D020245:p-Chloromercuribenzoic Acid; D019807:Iodoacetic Acid