Penetration of lysophosphatidylcholine into the dermis.
The ability of Lysophosphatidylcholine (LPC) to penetrate into the dermis and its degradation were investigated in vivo using hairless rats. Phosphatidylcholine (PC) was used as a control. Radioactively labelled LPC and PC were applied on the surface of skin, and the radioactivities of the epidermis and dermis were measured at 8, 24, and 48 hours. The recoveries of radioactive materials of LPC and PC within the area of epidermis and dermis at 8 hours were 0.21% and 0.25%; at 24 hours, 0.68% and 0.31%; and at 48 hours, 0.42% and 0.92%. No radioactivity was detected in serum. The radioactive substances which had penetrated were identified as LPC, PC, diglycerides, and free fatty acids. It was also found that topical application of LPC did not change the structure of skin as seen by microscopical examination. These findings indicate that a small amount of LPC can penetrate without the damaging skin structure and is enzymatically degraded into several lipids. Since LPC has bactericidal and antiviral activity, this substance could be an useful agent for dermatological use.
Uchida
Y
Y
Cosmetics Laboratory, Kanebo, LTD., Kanagawa, Japan.
Ogawa
T
T
Ohta
M
M
Kondo
M
M
Takada
S
S
Yamamura
M
M
D000818:Animals; D002855:Chromatography, Thin Layer; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005260:Female; D008244:Lysophosphatidylcholines / Q000493:pharmacokinetics*; D010713:Phosphatidylcholines / Q000493:pharmacokinetics; D011849:Radioactive Tracers; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000378:metabolism*
D005230:Fatty Acids, Nonesterified; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D011849:Radioactive Tracers