title: First expression of protamine message in trout testis.
abstract: In situ hybridizations were performed using a biotinylated riboprobe complementary to protamine messenger RNA in order to directly examine the various cell types in the trout testis for the presence of protamine message. Computer-aided optical density measurements were used to provide estimates of transcript abundance for cells identified by their DAPI-labeled nuclei. Optically detectable protamine hybridization occurred only in spermatid cells. These findings are in accord with results obtained in other species which report protamine mRNA only in the post-meiotic spermatid cell; but they are in conflict with a previous study employing solution hybridization which noted that protamine message first appears in the spermatocytes of rainbow trout.
authors.affiliation: Centre for Research in Neurodegenerative Diseases, University of Toronto, Ontario, Canada.
authors.forename: N A
authors.initials: NA
authors.lastname: Seniuk
authors.forename: W G
authors.initials: WG
authors.lastname: Tatton
authors.forename: P D
authors.initials: PD
authors.lastname: Cannon
authors.forename: A T
authors.initials: AT
authors.lastname: Garber
authors.forename: G H
authors.initials: GH
authors.lastname: Dixon
mesh_terms: D000818:Animals; D003720:Densitometry; D005455:Fluorescent Antibody Technique; D008297:Male; D011479:Protamines / Q000378:metabolism*; D015347:RNA Probes; D012333:RNA, Messenger / Q000378:metabolism; D012474:Salmon; D013087:Spermatids / Q000378:metabolism; D013090:Spermatocytes / Q000378:metabolism; D013093:Spermatogonia / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism*
chemical_list: D011479:Protamines; D015347:RNA Probes; D012333:RNA, Messenger