title: Interactions of Streptomyces serine-protease inhibitors with Streptomyces griseus metalloendopeptidase II.
abstract: Streptomyces griseus metalloendopeptidase II (SGMPII) was shown to form tight complexes with several Streptomyces protein inhibitors which had been believed to be specific to serine proteases, such as Streptomyces subtilisin inhibitor (SSI), plasminostreptin (PS), and alkaline protease inhibitor-2c' (API-2c'), as well as with Streptomyces metalloprotease inhibitor (SMPI). The dissociation constants of complexes between SGMPII and these inhibitors were successfully determined by using a novel fluorogenic bimane-peptide substrate. The values ranged from nM to pM. The results of studies by gel chromatographic and enzymatic analyses indicated that SGMPII is liberated from the complex with SSI by the addition of subtilisin BPN'. SGMPII and subtilisin BPN' proved, therefore, to interact with SSI in a competitive manner, despite the difference in the chemical nature of their active sites.
authors.affiliation: Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University.
authors.forename: K
authors.initials: K
authors.lastname: Kajiwara
authors.forename: A
authors.initials: A
authors.lastname: Fujita
authors.forename: H
authors.initials: H
authors.lastname: Tsuyuki
authors.forename: T
authors.initials: T
authors.lastname: Kumazaki
authors.forename: S
authors.initials: S
authors.lastname: Ishii
mesh_terms: D001483:Base Sequence; D001667:Binding, Competitive; D007527:Isoenzymes / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007700:Kinetics; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008969:Molecular Sequence Data; D009842:Oligopeptides; D011485:Protein Binding; D015842:Serine Proteinase Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D013302:Streptomyces / Q000378:metabolism*; D013305:Streptomyces griseus / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D007527:Isoenzymes; D009842:Oligopeptides; D015842:Serine Proteinase Inhibitors; D008666:Metalloendopeptidases