A spectrophotometric assay for lipase activity utilizing immobilized triacylglycerols.
New substrates for the determination of lipase activity have been developed. Triacylglycerols were immobilized by adsorption on an appropriate carrier or adsorbent yielding a lipase substrate in a powder form. The adsorbed triacylglycerols were easily hydrolyzed by lipases present in a reaction mixture. The released fatty acids were extracted with benzene and converted to the corresponding Cu (II) salts (copper soaps) which were measured spectrophotometrically.
Safarík
I
I
Laboratory of Analytical Chemistry, South Bohemian Biological Centre, Branisovská, Ceské Budĕjovice, Czechoslovakia.
D000327:Adsorption; D002175:Candida / Q000201:enzymology; D008049:Lipase / Q000378:metabolism*; D013053:Spectrophotometry; D014280:Triglycerides / Q000378:metabolism*
D014280:Triglycerides; D008049:Lipase