ABO blood grouping of semen from mixed body fluids with monoclonal antibody to tissue-specific epitopes on seminal ABO blood group substance.
Practical methods were developed for ABO blood grouping of semen from mixed body fluids. An monoclonal antibody (P6-5H) which recognizes a tissue-specific epitope on a seminal ABO blood group substance (alpha 2-seminoglycoprotein) was used as a solid phase antibody for selective capture of the seminal ABO blood group substance. ABO blood group epitopes of secretor and non-secretor semen were detected in dilutions of 8 x 10(3)-3.2 x 10(4) and 8 x 10(3)-fold, respectively, by sandwich ELISA. ABO blood group epitopes were also detected in dilutions up to 4 x 10(3)-fold, irrespective of secretor status, by the sandwich absorption-elution test.
Kimura
A
A
Department of Legal Medicine, Wakayama Medical College, Japan.
Matsumura
F
F
Sodesaki
K
K
Osawa
M
M
Ikeda
H
H
Yasuda
S
S
Tsuji
T
T
D000017:ABO Blood-Group System / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D001788:Blood Grouping and Crossmatching / Q000379:methods* / Q000592:standards; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005069:Evaluation Studies as Topic; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology*; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000276:immunology*; D012661:Semen / Q000276:immunology*; D029589:Seminal Plasma Proteins
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D016056:Immunodominant Epitopes; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein