title: Transglycosylation activity of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae.
abstract: Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae has transglycosylation activity. Treatment of (Man)6(GlcNAc)2Asn with the enzyme in the presence of free N-acetylglucosamine gave a mixture of (Man)6GlcNAc and (Man)6GlcNAc beta 1----4GlcNAc mixture. N-Acetylglucosamine at the reducing end of the latter sugar chain was found by HPLC of the carbohydrate composition and of an exoglycosidase digest of the pyridylamino derivative of the reducing-end residue, and by 400 MHz 1H-NMR spectroscopy.
authors.affiliation: Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takegawa
authors.forename: S
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: A
authors.initials: A
authors.lastname: Kondo
authors.forename: H
authors.initials: H
authors.lastname: Iwamoto
authors.forename: M
authors.initials: M
authors.lastname: Nakoshi
authors.forename: I
authors.initials: I
authors.lastname: Kato
authors.forename: S
authors.initials: S
authors.lastname: Iwahara
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism; D001173:Arthrobacter / Q000201:enzymology*; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006031:Glycosylation; D009682:Magnetic Resonance Spectroscopy; D008361:Mannosidases / Q000378:metabolism; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / Q000378:metabolism*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism*; D043323:alpha-Mannosidase
chemical_list: D009844:Oligosaccharides; D008361:Mannosidases; D043323:alpha-Mannosidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D000117:Acetylglucosamine