Blood flow dependent granulocyte activation in membranes with and without complement activation.
Synthetic membranes activate the complement system to some degree but also clear complement fragments from the circulation by adsorption and/or filtration and dialysis, PAN and PMMA even lower preexisting plasma levels of C3a. Activation of granulocytes measured as elastase release does occur with all membranes and might be due to complement activation. However, in synthetic membranes no correlation exists between levels of complement fragment C3a and elastase release. Instead, even when PMMA membrane lowered preexisting plasma levels of the C3a below baseline, activation of granulocytes to a similar degree as known from cuprophane membranes was observed. This activation did occur with blood flow of 200 ml/min during recirculation, but not without blood flow during incubation of membrane material. We conclude that granulocyte activation in synthetic membranes does not require an activated complement system, but may be a blood flow dependent phenomenon.
Böhler
J
J
Department of Medicine, University of Freiburg, FRG.
Donauer
J
J
Schollmeyer
P
P
Hörl
W H
WH
D000180:Acrylic Resins; D001783:Blood Flow Velocity; D002482:Cellulose / Q000031:analogs & derivatives; D003167:Complement Activation; D015926:Complement C3a / Q000378:metabolism; D006098:Granulocytes / Q000502:physiology*; D006801:Humans; D008567:Membranes, Artificial; D008768:Methylmethacrylates; D010196:Pancreatic Elastase / Q000097:blood; D011108:Polymers; D006435:Renal Dialysis / Q000295:instrumentation*; D013450:Sulfones
D000180:Acrylic Resins; D008567:Membranes, Artificial; D008768:Methylmethacrylates; D011108:Polymers; D013450:Sulfones; C010504:polyacrylonitrile; C017662:polysulfone P 1700; D015926:Complement C3a; D002482:Cellulose; C009865:cuprammonium cellulose; D010196:Pancreatic Elastase