title: High-performance liquid chromatographic determination of taurine in biological fluids by post-column fluorescence reaction with thiamine.
abstract: A high-performance liquid chromatographic method is described for the selective determination of taurine in biological fluids by post-column fluorescence reaction. Taurine was separated on an adsorption-distribution type Shodex Ionpac KC-811 column. Then it was converted with hypochlorite into the corresponding N-chloramine, which was allowed to react with thiamine to give fluorescent thiochrome. As little as 6 ng per injection of taurine could be determined. The average recoveries of spiked taurine in serum and urine were 99.5 +/- 2.7 and 101.8 +/- 2.9%, respectively. The method could be applied to the assay of taurine in human serum and urine with simple pretreatment.
authors.affiliation: Central Research Laboratory, SS Pharmaceutical Co. Ltd., Chiba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yokoyama
authors.forename: T
authors.initials: T
authors.lastname: Kinoshita
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D008297:Male; D013654:Taurine / Q000097:blood* / Q000652:urine*; D013831:Thiamine
chemical_list: D013654:Taurine; D013831:Thiamine