Structure and sequence analysis of the human activin beta A subunit gene.
The cloned genomic DNA containing the human activin beta A subunit gene were analyzed by restriction endonuclease mapping, Southern blotting and DNA sequencing. The activin gene is composed of two exons interrupted by the 9-kb intron. The TATA, CCAAT and CT-stretch sequences were found in the 5'-flanking region of the gene. An intronic sequence contained SV40 enhancer core element in the vicinity of the exon 1. In the 3'-flanking region, we identified eight consensus polyadenylation sequences, five ATTTA motifs, CA element consisting of (CA)14, AP-1 binding site and two SV40 enhancer core elements. A dot matrix analysis revealed the high degree of conservation between the human and rat sequences within the 3'-flanking region, suggesting a possible functional significance.
Tanimoto
K
K
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Handa
S
S
Ueno
N
N
Murakami
K
K
Fukamizu
A
A
D028341:Activins; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA; D005786:Gene Expression Regulation; D006801:Humans; D007265:Inhibins / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D015183:Restriction Mapping
D028341:Activins; D007265:Inhibins; D004247:DNA