title: Anticryptococcal activity of amphotericin B-stimulated macrophages.
abstract: Amphotericin B (AmB) and its methyl ester derivative (AME) are immunoadjuvants with macrophage stimulating properties. Cultures containing AmB and murine peritoneal macrophages showed synergistic anticryptococcal activity. The antifungal activity was associated with AmB-stimulated macrophages and with their culture supernatants. Photoinactivation of the residual AmB in the macrophage culture supernatant did not result in the loss of antifungal activity. AmB-stimulated macrophage culture supernatants inhibited the growth of C. neoformans in a dose responsive manner and the activity was destroyed by incubation at 100 degrees C but not at 60 degrees C.
authors.affiliation: Department of Medicine, Jewish Hospital, St. Louis.
authors.forename: J
authors.initials: J
authors.lastname: Aslanzadeh
authors.forename: J S
authors.initials: JS
authors.lastname: Mormol
authors.forename: J R
authors.initials: JR
authors.lastname: Little
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D003455:Cryptococcus neoformans / Q000187:drug effects* / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D005260:Female; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology; D051379:Mice
chemical_list: D000666:Amphotericin B