Molecular cloning and nucleotide sequence of cDNA encoding the human liver S-adenosylmethionine synthetase.
cDNA clone for human liver S-adenosylmethionine synthetase (liver-specific isoenzyme) was isolated from a cDNA library of human liver poly(A)+ RNA. The cDNA sequence encoded a polypeptide consisting of 395 amino acid residues with a calculated molecular mass of 43675 Da. Alignment of the predicted amino acid sequence of this protein with that of rat liver S-adenosylmethionine synthetase showed a high degree of similarity. The coding region of the human liver S-adenosylmethionine synthetase cDNA sequence was 89% identical at the nucleotide level and 95% identical at the amino acid level to the sequence for rat liver S-adenosylmethionine synthetase.
Horikawa
S
S
Department of Pathological Biochemistry, Tokyo Medical and Dental University, Japan.
Tsukada
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D008716:Methionine Adenosyltransferase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D051381:Rats; D015183:Restriction Mapping; D016415:Sequence Alignment
D004247:DNA; D008716:Methionine Adenosyltransferase