Specific antibodies against the stress-inducible 72-kDa protein, a member of the heat-shock protein hsp70, in healthy human subjects.
1. On Western blot analysis, serum IgG from a healthy human subject reacted with a stress-induced protein, having an apparent molecular mass of 70 kDa, from PC12 cells. 2. This serum IgG also reacted with hsp70 (70-kDa heat-shock protein) purified from the bovine brain. 3. In 5 out of 34 healthy human subjects, IgG antibodies against hsp70 were detected. 4. These antibodies were directed against the stress-inducible 72-kDa protein, but did not cross-react with the constitutive 73-kDa protein.
Wakui
H
H
Third Department of Internal Medicine, Akita University School of Medicine, Japan.
Itoh
H
H
Tashima
Y
Y
Kobayashi
R
R
Nakamoto
Y
Y
Miura
A B
AB
D000328:Adult; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D015153:Blotting, Western; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D006360:Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D008875:Middle Aged; D016716:PC12 Cells; D051381:Rats
D000906:Antibodies; D006360:Heat-Shock Proteins