Increased concentrations of endogenous 13-cis- and all-trans-retinoic acids in diffuse idiopathic skeletal hyperostosis, as demonstrated by HPLC.
Endogenous 13-cis- and all-trans-retinoic acids have been quantitated in human serum using a solvent extraction procedure followed by isocratic reversed phase high performance liquid chromatography and UV detection. In healthy adults, after an overnight fasting period, the concentrations of 13-cis- and all-trans-retinoic acids yielded 5.3 +/- 2.43 nmol/l and 11.8 +/- 3.3 nmol/l, respectively (mean +/- SD). The method has been successfully applied to the analysis of both isomers in serum from patients with idiopathic skeletal hyperostosis in whom, the 13-cis- as well as all-trans-retinoic acid levels were raised as compared to the control group.
Periquet
B
B
Laboratorie de Biochimie, CHU Purpan, Toulouse, France.
Lambert
W
W
Garcia
J
J
Lecomte
G
G
De Leenheer
A P
AP
Mazieres
B
B
Thouvenot
J P
JP
Arlet
J
J
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D004057:Hyperostosis, Diffuse Idiopathic Skeletal / Q000097:blood*; D015474:Isotretinoin / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012177:Retinol-Binding Proteins / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D014212:Tretinoin / Q000097:blood*; D014801:Vitamin A / Q000097:blood
D012177:Retinol-Binding Proteins; D014801:Vitamin A; D014212:Tretinoin; D015474:Isotretinoin