Malaria screening to prevent transmission by transfusion: an evaluation of techniques.
Transfusion-associated malaria is often severe or even fatal, because diagnosis is frequently delayed and it complicates an already serious underlying disorder. Detection of infected donors is difficult in endemic areas due to the lack of a suitable donor screening test. Blood smear staining techniques show poor results due to the low parasite concentration in many infected persons, and the antibody detection test is not helpful due to the universal presence of antibody in healthy donors in these areas. For comparative evaluation of various screening tests, 9131 blood smears from voluntary donors and a group of patients were screened by Giemsa staining. Ten (0.10%) subjects showed parasitaemia, whereas Acridine Orange fluorescence staining showed 13 (0.14%) parasitaemia in almost the same number of smears screened on the same samples. Significantly high levels of malarial antibody were detected in 12.6% and 19.86% of subjects by indirect fluorescent antibody and enzyme-linked immunoassay tests, respectively. Malarial antigen detection by monoclonal antibody showed positive results in 9.48% of subjects, demonstrating excellent results and showing direct evidence of infection. We recommend that this should be adopted as a screening technique by transfusion services in endemic areas in order to prevent post transfusion malaria.
Choudhury
N
N
Dept of Transfusion Medicine, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.
Jolly
J G
JG
Mahajan
R C
RC
Ganguly
N K
NK
Dubey
M L
ML
Agnihotri
S K
SK
D000165:Acridine Orange; D000913:Antibodies, Protozoan / Q000032:analysis; D001399:Azure Stains; D001782:Blood Donors; D006801:Humans; D008288:Malaria / Q000097:blood / Q000517:prevention & control*; D008403:Mass Screening
D000913:Antibodies, Protozoan; D001399:Azure Stains; D000165:Acridine Orange