Escherichia hermannii (ATCC 33651) polysaccharide-protein conjugates: comparison of two conjugation methods for the induction of humoral responses in mice.
Escherichia hermannii (ATCC 33651) LPS O-polysaccharide was covalently linked to a carrier (bovine serum albumin) to form conjugates either directly or with a spacer arm (adipic acid dihydrazide). The immunogenicity of both conjugates at three different doses was tested in mice. Antibodies to the conjugate were produced and were shown to react with free lipopolysaccharide. The directly-coupled conjugate was found to be more immunogenic than the indirect one (i.e. lower dose necessary for a similar response). The antibody response elicited by the directly coupled conjugate (1 microgram/animal) began at 21 days and was sustained for at least 4 months. The mouse model described here may be applicable to the testing of other conjugates composed of bacterial cell wall polysaccharides and LPS O-chains.
Jacques
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Institut National de la Recherche Agronomique, Station de Pathologie de la Reproduction, Nouzilly, France.
Dubray
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D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D004925:Escherichia / Q000276:immunology*; D005260:Female; D007114:Immunization; D051379:Mice; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
D000907:Antibodies, Bacterial; D011135:Polysaccharides, Bacterial; D012710:Serum Albumin, Bovine