Ascorbic acid derivatives in two different fractions of uremic toxins.
The middle-molecular-weight uremic toxins which accumulate in uremic plasma seem to be associated with various uremic disorders such as uremic neuropathy and defects in the sodium pump. By a multi-step chromatographic method, two fractions of these toxins were isolated and studied because one inhibits microtubule formation in vitro (fraction 2-5), and the other impairs the sodium pump in living erythrocytes (fraction 2-3). An additional chromatographic method allows the separation of these fractions and isolation of two components: fractions 2-3-V and 2-5-III. Analyses by UV and 1H NMR spectrometry identified these compounds as two different ascorbic acid derivatives. 2-3-V is not yet totally identified and 2-5-III corresponds to ascorbic acid 2-sulfate. These two metabolites exert no toxic effects but they have the same chromatographic behavior as uremic toxins.
Gallice
P M
PM
Biophysics Laboratory, UFR de Pharmacie, Marseille, France.
Monti
J P
JP
Braguer
D L
DL
Baz
M
M
Elsen
R E
RE
Berland
Y F
YF
Crevat
A D
AD
D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet; D014118:Toxins, Biological / Q000737:chemistry*; D014511:Uremia / Q000097:blood*
D014118:Toxins, Biological; C026865:uremia middle molecule toxins; D001205:Ascorbic Acid