title: Plastid DNA synthesis and nucleic acid-binding proteins in developing barley chloroplasts.
abstract: Activation of plastid DNA synthesis occurred early in barley leaf chloroplast development. High rates of DNA synthesis were observed in the leaf basal meristem of dark-grown plants where plastid transcription activity was low. DNA synthesis activity decreased in later stages of chloroplast development. Plastid nuclei acid-binding proteins were detected after lithium dodecylsulfate (LDS)-polyacrylamide gel separation and renaturation. One set of nucleic acid-binding proteins was associated with plastid nucleoids. A second set of nucleic acid-binding proteins co-sedimented with ribosomes and most probably corresponds to ribosomal proteins. Changes in the composition of the nucleic acid-binding proteins were characterized as a function of chloroplast development in dark-grown and illuminated barley plants.
authors.affiliation: Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843-2128.
authors.forename: B J
authors.initials: BJ
authors.lastname: Baumgartner
authors.forename: J E
authors.initials: JE
authors.lastname: Mullet
mesh_terms: D002736:Chloroplasts / Q000187:drug effects / Q000502:physiology*; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D003624:Darkness; D004591:Electrophoresis, Polyacrylamide Gel; D001467:Hordeum / Q000502:physiology*; D008027:Light; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D004247:DNA