Proline enhances primary photochemical activities in isolated thylakoid membranes of Brassica juncea by arresting photoinhibitory damage.
The presence of L-proline in the reaction mixture enhances the photosystem II (H2O----DCPIP) and whole chain (H2O----MV) catalysed electron transport activities of thylakoids isolated from the cotyledonary leaves of Brassica juncea seedlings raised in the absence and the presence of NaCl. The extent of stimulation in activities was higher in the thylakoids of NaCl raised plants than the controls. The extent of proline mediated stimulation was seen even in the presence of uncoupler NH4Cl suggesting that this stimulation is not due to uncoupling. However, photosystem I (DCPIPH2----MV) catalysed photoreaction remained almost insensitive to proline. The presence of proline in the incubation medium brought about a significant reduction in the time dependent loss in photochemical activity of thylakoids exposed to strong light suggesting that proline prevents photoinhibitory loss in chloroplast activity. Also, proline brought about a considerable reduction in the production of lipid peroxidation linked maiondialdehyde during strong illumination. We suggest that proline protects the components involved in water oxidation capacity by reducing the production of free radicals and/or scavenging the free radicals and thereby reducing thylakoid lipid peroxidation.
Alia
Centre for Biosciences, New Delhi, India.
Saradhi
P P
PP
Mohanty
P
P
D000643:Ammonium Chloride / Q000494:pharmacology; D001937:Brassica / Q000378:metabolism*; D004579:Electron Transport / Q000187:drug effects; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D007700:Kinetics; D008027:Light; D008315:Malondialdehyde / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D011392:Proline / Q000494:pharmacology*
D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D000643:Ammonium Chloride; D008315:Malondialdehyde; D011392:Proline