title: Mössbauer spectroscopic investigation of structure-function relations in ferritins.
abstract: Ferritin plays an important role in iron metabolism and our aim is to understand the mechanisms by which iron is sequestered within its protein shell as the mineral ferrihydrite. We present Mössbauer spectroscopic data on recombinant human and horse spleen ferritin from which we draw the following conclusions: (1) that apoferritin catalyses Fe(II) oxidation as a first step in ferrihydrite deposition, (2) that the catalysis of Fe(II) oxidation is associated with residues situated within H chains, at the postulated 'ferroxidase centre' and not in the 3-fold inter-subunit channels previously suggested as the initial Fe(II) binding and oxidation site; (3) that both isolated Fe(III) and Fe(III) mu-oxo-bridged dimers found previously by Mössbauer spectroscopy to be intermediates in iron-core formation in horse spleen ferritin, are located on H chains; and (4) that these dimers form at ferroxidase centres. The importance of the ferroxidase centre is suggested by the conservation of its ligands in many ferritins from vertebrates, invertebrates and plants. Nevertheless iron-core formation does occur in those ferritins that lack ferroxidase centres even though the initial Fe(II) oxidation is relatively slow. We compare the early stages of core formation in such variants and in horse spleen ferritin in which only 10-15% of its chains are of the H type. We discuss our findings in relation to the physiological role of isoferritins in iron storage processes.
authors.affiliation: Racah Institute of Physics, Hebrew University, Jerusalem, Israel.
authors.forename: E R
authors.initials: ER
authors.lastname: Bauminger
authors.forename: P M
authors.initials: PM
authors.lastname: Harrison
authors.forename: D
authors.initials: D
authors.lastname: Hechel
authors.forename: I
authors.initials: I
authors.lastname: Nowik
authors.forename: A
authors.initials: A
authors.lastname: Treffry
mesh_terms: D000818:Animals; D001059:Apoproteins / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D002384:Catalysis; D004252:DNA Mutational Analysis; D005290:Ferric Compounds / Q000737:chemistry; D005293:Ferritins / Q000737:chemistry*; D005296:Ferrous Compounds / Q000737:chemistry; D005971:Glutamates / Q000737:chemistry; D006736:Horses; D006801:Humans; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000737:chemistry; D015204:Spectroscopy, Mossbauer; D013329:Structure-Activity Relationship
chemical_list: D001059:Apoproteins; D005290:Ferric Compounds; D005296:Ferrous Compounds; D005971:Glutamates; D011994:Recombinant Proteins; D005293:Ferritins