title: Flow effects on cultured vascular endothelial and smooth muscle cell functions.
abstract: Cultured vascular endothelial cells were exposed to fluid shear stress by means of a rotary-disc shear-loading device, and the physiological effects of the conditioned medium (CM) and the homogenate (HM) of the cells on migration, adhesion and growth of endothelial cells (EC) or smooth muscle cells (SMC) were studied. Effects of shear stress on the production and secretion of collagen, one of the extracellular matrices of EC, were also studied. CM stimulated the adhesion and growth of SMC, but not of EC themselves. The ability to stimulate SMC adhesion and growth was similar in CM obtained from the static and shear-loaded cells. HM of the shear-loaded EC stimulated SMC migration. Further, HM of the shear-loaded EC contained increased amounts of collagen compared with the static EC. These results suggest that: 1) EC produce and secrete accelerators for the adhesion and growth of SMC, 2) EC react to the physical stimulus of fluid shear stress to produce stimulators of SMC migration, and 3) EC produce collagen, the production of which is enhanced by fluid shear stress.
authors.affiliation: Department of Cardiovascular Medicine, School of Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Ono
authors.forename: J
authors.initials: J
authors.lastname: Ando
authors.forename: A
authors.initials: A
authors.lastname: Kamiya
authors.forename: Y
authors.initials: Y
authors.lastname: Kuboki
authors.forename: H
authors.initials: H
authors.lastname: Yasuda
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000254:growth & development; D008858:Microscopy, Phase-Contrast; D024510:Muscle Development; D009130:Muscle, Smooth / Q000166:cytology* / Q000254:growth & development; D013314:Stress, Mechanical
chemical_list: D003470:Culture Media