Complementation of the inability of Lactobacillus strains to utilize D-xylose with D-xylose catabolism-encoding genes of Lactobacillus pentosus.
The inability of two Lactobacillus strains to ferment D-xylose was complemented by the introduction of Lactobacillus pentosus genes encoding D-xylose isomerase, D-xylulose kinase, and a D-xylose catabolism regulatory protein. This result opens the possibility of using D-xylose fermentation as a food-grade selection marker for Lactobacillus spp.
Posno
M
M
TNO Medical Biological Laboratory, Rijswijk, The Netherlands.
Heuvelmans
P T
PT
van Giezen
M J
MJ
Lokman
B C
BC
Leer
R J
RJ
Pouwels
P H
PH
D019747:Aldose-Ketose Isomerases; D002238:Carbohydrate Epimerases / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D007778:Lactobacillus / Q000235:genetics* / Q000254:growth & development; D014994:Xylose / Q000235:genetics / Q000378:metabolism*
D014994:Xylose; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase