Influence of innervation of myogenic factors and acetylcholine receptor alpha-subunit mRNAs.
The mRNA levels of the acetylcholine receptor alpha-subunit gene and of four members of the MyoD1 gene family, which code for candidate transcriptional activators of the former gene, were compared in the mouse during post-natal development and after denervation in the adult. During post-natal development, mRNA levels of myogenin, MyoD1, and Myf5 decrease in parallel with alpha-subunit mRNA whereas MRF4 mRNA level remains nearly constant. After denervation, increases in the levels of mRNAs coding for myogenin, MyoD1 and MRF4 accompany that of alpha-subunit mRNA, while Myf5 mRNA level varied little. A possible role of these myogenic genes in mediating nerve influence on the state of muscle differentiation and AChR alpha-subunit gene expression is discussed.
Duclert
A
A
U.A. CNRS D1284, Neurobiologie Moléculaire, Département des Biotechnologies, Institut Pasteur, Paris, France.
Piette
J
J
Changeux
J P
JP
D000818:Animals; D000831:Animals, Newborn; D003714:Denervation; D006706:Homeostasis; D051379:Mice; D024510:Muscle Development; D009124:Muscle Proteins / Q000235:genetics*; D017570:MyoD Protein; D009424:Nervous System Physiological Phenomena; D009687:Nuclear Proteins / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics
D009124:Muscle Proteins; D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; D009687:Nuclear Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D011950:Receptors, Cholinergic; D015534:Trans-Activators