title: Electric field stimulation of human osteosarcoma-derived cells: a dose-response study.
abstract: In vitro electrical stimulation of human osteosarcoma-derived cells resulted in increased cell adherence and current directed cell migration. We have developed an electrical exposure system in which two steel electrodes imbedded in media-based agar, poured in a standard culture dish, are used to apply electric field signals to cells in culture without ion contamination from the electrodes. The cells were exposed to a 100 Hz pulsed DC electric signal at peak field strengths of 1, 10, 100, and 625 mV/cm in the culture media. The data showed no change in cell adherence at 1 and 10 mV/cm, an increase in adherence at 100 mV/cm, and a decrease in both adherence and cell proliferation at 625 mV/cm. Electric field stimulation in vivo has been found useful in accelerating the healing of fractures and non-unions, and the repair of surgical and cancer-related skeletal defects.
authors.affiliation: Orthopaedic Research Laboratory, V.A. Medical Center, Omaha, NE 68105.
authors.forename: R J
authors.initials: RJ
authors.lastname: Naegele
authors.forename: J
authors.initials: J
authors.lastname: Lipari
authors.forename: D
authors.initials: D
authors.lastname: Chakkalakal
authors.forename: B
authors.initials: B
authors.lastname: Strates
authors.forename: M
authors.initials: M
authors.lastname: McGuire
mesh_terms: D055592:Biophysical Phenomena; D001703:Biophysics; D002448:Cell Adhesion; D002455:Cell Division; D002465:Cell Movement; D004558:Electric Stimulation; D004560:Electricity; D006801:Humans; D012516:Osteosarcoma / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000473:pathology*