title: The complete amino-acid sequence of the heavy chain of the human myeloma protein WIE, an immunoglobulin D.
abstract: The human myeloma protein WIE is a lambda-type immunoglobulin D; the amino-acid sequence of its Fc part and aminoethylated heavy chain was completely determined. The VH-part (subgroup III) begins N-terminally with 5-oxoproline, and it contains a long, unique CDR3 region. Since the constant part differs from known delta chains by one amino-acid substitution in the hinge region, IgD WIE probably represents an allotypic variant. As in other protein delta chains, O-glycosylations are confined to the hinge region. Furthermore, the ratios of N-glycosylations at the three positions are identical in IgD WAH [Takahashi, N. et al. (1984) J. Chromatogr. 317, 11-26.] and IgD WIE (100%, 50%, 100%). From the most conserved constant domain, C delta 3, a three-dimensional model was constructed to clarify the role of its delta-specific substitutions and glycosylation.
authors.affiliation: Max-Planck-Institut für Experimentelle Medizin, Abteilung Immunchemie, Göttingen.
authors.forename: R J
authors.initials: RJ
authors.lastname: Friedrich
authors.forename: R
authors.initials: R
authors.lastname: Bätge
authors.forename: I
authors.initials: I
authors.lastname: Schranner
authors.forename: U
authors.initials: U
authors.lastname: Kotucha
authors.forename: K
authors.initials: K
authors.lastname: Eckart
authors.forename: N
authors.initials: N
authors.lastname: Hilschmann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D006801:Humans; D007122:Immunoelectrophoresis; D007072:Immunoglobulin D / Q000737:chemistry / Q000235:genetics*; D007128:Immunoglobulin Fragments / Q000302:isolation & purification; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009194:Myeloma Proteins / Q000737:chemistry / Q000235:genetics*; D011487:Protein Conformation; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D007072:Immunoglobulin D; D007128:Immunoglobulin Fragments; D007143:Immunoglobulin Heavy Chains; D009194:Myeloma Proteins; C072624:myeloma protein WIE