Carbon-phosphorus bond cleavage activity in cell-free extracts of Enterobacter aerogenes ATCC 15038 and Pseudomonas sp. 4ASW.
Carbon-phosphorus bond cleavage activity was investigated in cell-free extracts of Enterobacter aerogenes ATCC 15038 (IFO 12010) and Pseudomonas sp. 4ASW, strains known to utilize a range of phosphonates as sole phosphorus source. In vitro phosphonatase activity was detected in extracts of both organisms; however extensive analysis failed to detect any organic product from phosphonates other than phosphonoacetal dehyde. Non-specific liberation of phosphate was observed in Pseudomonas sp. 4ASW, associated with a single fraction of FPLC-purified extract, and is believed to result from the activity of cellular phosphatases.
McMullan
G
G
School of Biology and Biochemistry, Queens University, Belfast.
Watkins
R
R
Harper
D B
DB
Quinn
J P
JP
D002851:Chromatography, High Pressure Liquid; D004754:Enterobacter / Q000201:enzymology*; D006867:Hydrolases / Q000378:metabolism; D008190:Lyases / Q000378:metabolism; D063065:Organophosphonates / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology*
D063065:Organophosphonates; D010710:Phosphates; D006867:Hydrolases; C023920:phosphonoacetaldehyde hydrolase; D008190:Lyases; C051328:carbon-phosphorus lyase