Comparative study on the plaque forming cell response by two different methods of plaque assay.
The Cunningham and Sezenberg Technique (using mice sensitized lymphoid cells to SRBCs) was tested together with Trump's gel technique (using non sensitized mice lymphoid cell mixed with SRBCs and incubated in Marbrook culture chamber for sensitization). The study showed that the number of PFCs by the Cunningham slid was greater than that observed by the gel technique of Trump at the same lymphocyte concentration. Also it was observed that the Trump's method using the Marbrook tissue culture chamber was a time consuming technique than that obtained by the Cunningham technique.
Arafa
R M
RM
Dept. Microbiology, Benha Faculty of Medicine, Egypt.
D000818:Animals; D003588:Cytopathogenic Effect, Viral / Q000502:physiology*; D005069:Evaluation Studies as Topic; D051379:Mice; D013997:Time Factors; D010948:Viral Plaque Assay / Q000379:methods / Q000592:standards*