title: Physical and genetic map of the Neisseria gonorrhoeae strain MS11-N198 chromosome.
abstract: A macro-restriction map of the Neisseria gonorrhoeae chromosome was constructed using the enzymes Nhel and Spel. Combinations of one- and two-dimensional electrophoresis of completely or partially digested chromosomal DNA were performed to align the restriction fragments. The chromosome is circular, with an estimated size of 2.33 Mb +/- 35 kb. A genetic map was derived from the physical map; positions of over 60 defined loci were determined by Southern hybridization.
authors.affiliation: Max-Planck-Institut für Biologie, Abteilung Infektionsbiologie, Tübingen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Bihimaier
authors.forename: U
authors.initials: U
authors.lastname: Römling
authors.forename: T F
authors.initials: TF
authors.lastname: Meyer
authors.forename: B
authors.initials: B
authors.lastname: Tümmler
authors.forename: C P
authors.initials: CP
authors.lastname: Gibbs
mesh_terms: D015139:Blotting, Southern; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D010957:Plasmids; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial