Comparison of rat and human major platelet glycoproteins.
1. Using electrophoretic techniques combined with various detection methods we ascribed rat platelet glycoproteins (GPs) related to human GPIb, GPIIb and GPIIIa. 2. Rat GPIIb and GPIIIa crossreacted with rabbit polyclonal antibodies against human GPIIb and GPIIIa. 3. Species differences in glycosylation of GPs were shown using various lectins. 4. Molecular mass of rat major GPs was determined by SDS-PAGE (unreduced, reduced, kDa): GPIb (200, 166/26), GPIIb (140, 120/32) and GPIIIa (96, 106). 5. Isoelectric points of rat GPIIb and GPIIIa are shifted to the alkaline region as compared to human related GPs.
Holada
K
K
Institute of Hematology and Blood Transfusion, Praha, Czechoslovakia.
Dyr
J E
JE
Suttnar
J
J
Simák
J
J
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008970:Molecular Weight; D010980:Platelet Membrane Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D010980:Platelet Membrane Glycoproteins