title: Repression of a mating type cassette in the fission yeast by four DNA elements.
abstract: The fission yeast, Schizosaccharomyces pombe, expresses one of two alternative mating types. They are specified by one of two determinants (M or P) present at the mat1 locus. In addition, silent copies of M and P are present on the same chromosome. In the present work we demonstrate that the difference between the active and the silent stage of the P determinant is controlled by four repressive elements that are located at the silent locus. There are two elements to the left and two to the right of the mating type cassette. Both elements to the left and either one of the two elements to the right are required for an effective blockage of transcription. When they are combined, the four elements define a highly efficient silencer functionally similar to the HMRE and HMLE and HMLI silencers in Saccharomyces cerevisiae. In addition, the DNA surrounding the silent P locus confers symmetric partitioning in mitosis to Schizosaccharomyces pombe ars plasmids.
authors.affiliation: Department of Molecular Biology, University of Uppsala, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Ekwall
authors.forename: O
authors.initials: O
authors.lastname: Nielsen
authors.forename: T
authors.initials: T
authors.lastname: Ruusala
mesh_terms: D004271:DNA, Fungal / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D008938:Mitosis; D010957:Plasmids; D012045:Regulatory Sequences, Nucleic Acid; D012568:Schizosaccharomyces / Q000235:genetics* / Q000502:physiology
chemical_list: D004271:DNA, Fungal