title: Three DNA sequencing methods using capillary gel electrophoresis and laser-induced fluorescence.
abstract: Capillary gel electrophoresis is demonstrated for the four-spectral-channel sequencing technique of Smith, the two-spectral-channel sequencing technique of Prober, and the one-spectral-channel sequencing technique of Richardson and Tabor. Sequencing rates up to 1000 bases/h are obtained at electric field strengths of 465 V/cm. At lower electric field strengths, capillary electrophoresis produces useful data for fragments greater than 550 nucleotides in length with 2 times better resolution than slab gel electrophoresis. An on-column detector produces detection limits of 200 zmol (1 zmol = 10(-21) mol = 600 molecules) for the four-spectral-channel technique. A postcolumn detector, based on the sheath flow cuvette, produces detection limits of 20 and 2 zmol for the two- and one-spectral-channel techniques, respectively.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Swerdlow
authors.forename: J Z
authors.initials: JZ
authors.lastname: Zhang
authors.forename: D Y
authors.initials: DY
authors.lastname: Chen
authors.forename: H R
authors.initials: HR
authors.lastname: Harke
authors.forename: R
authors.initials: R
authors.lastname: Grey
authors.forename: S L
authors.initials: SL
authors.lastname: Wu
authors.forename: N J
authors.initials: NJ
authors.lastname: Dovichi
authors.forename: C
authors.initials: C
authors.lastname: Fuller
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D004586:Electrophoresis; D005453:Fluorescence; D007834:Lasers
chemical_list: D004247:DNA