Analysis of human blood plasma triacylglycerols using capillary gas chromatography, silver ion thin-layer chromatographic fractionation and desorption chemical ionization mass spectrometry.
The identification and quantitation from pooled human plasma of over 50 molecular species of triacylglycerols, including 22 not previously reported, are reported. The triacylglycerols were first resolved by silver ion thin-layer chromatography into seven fractions, which were independently analysed by polarizable capillary gas chromatography and desorption chemical ionization mass spectrometry in the presence of an internal standard. The two methods gave similar values for the estimates of oligoenoic species, but the former method underestimated the polyenes (due mainly to significant losses caused by thermal degradation), and the latter method overestimated the saturates. The results show that an effective analysis of molecular species of plasma triacylglycerols cannot be obtained by either technique alone.
Mares
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IV Department of Medicine, Charles University, Prague, Czechoslovakia.
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Novák
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D002849:Chromatography, Gas / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D014280:Triglycerides / Q000097:blood*
D014280:Triglycerides