Comparison of the adhesion properties of Deleya marina and the exopolysaccharide-defective mutant strain DMR.
Deleya marina 219 (ATCC 25374) produces large quantities of an acidic exopolysaccharide and characteristically forms mucoid colonies and large aggregates of cells. The exopolysaccharide of wild-type D. marina cells appears to occur as both film and fibrils in electron micrographs. The organization of exopolymeric material was indicative of structural heterogeneity. A spontaneous rough-colony mutant defective in exopolysaccharide, D. marina DMR, has been isolated. The absence of exopolymer corresponds to a nonmucoid, nonaggregating, adhesion-altered phenotype. In microplate adhesion assays, wild-type cells grown at 19 or 25 degrees C attached to hydrophilic surfaces but not to a hydrophobic surface. In contrast, mutant cells exhibited a significantly reduced level of attachment to hydrophilic surfaces and increased adhesion to a hydrophobic surface.
Shea
C
C
Department of Biological Sciences, University of Alabama, Tuscaloosa 35487.
Nunley
J W
JW
Williamson
J C
JC
Smith-Somerville
H E
HE
D001419:Bacteria / Q000235:genetics / Q000648:ultrastructure; D001422:Bacterial Adhesion; D018407:Bacterial Physiological Phenomena; D015169:Colony Count, Microbial; D008855:Microscopy, Electron, Scanning; D009154:Mutation; D010641:Phenotype; D011135:Polysaccharides, Bacterial / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure
D011135:Polysaccharides, Bacterial