Regulation of epidermal growth factor receptors in rat gastric mucosa: effect of gastric ulceration.
The effect of experimental ulcers on the binding of human epidermal growth factor (hEGF), beta-urogastrone, to plasma membranes isolated from rat gastric mucosa was studied in order to develop hEGF as an anti-ulcer drug. The binding of [125I]hEGF to the gastric plasma membranes was significantly decreased 1 h after treatment with aspirin (300 mg/kg) and after 12 h of water-immersion stress. However, the number of EGF binding sites was increased 12 h after aspirin administration. There was little change in the binding of [125I]insulin to the gastric plasma membranes in response to water-immersion stress. These results indicate that changes in EGF binding to its receptors occur on plasma membranes of the rat gastric mucosa during ulcer.
Nomura
H
H
Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Japan.
Iwakawa
S
S
Saito
Y
Y
Hori
R
R
Okumura
K
K
D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000032:analysis*; D005753:Gastric Mucosa / Q000737:chemistry*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013276:Stomach Ulcer / Q000378:metabolism*
D004815:Epidermal Growth Factor; D004247:DNA; D066246:ErbB Receptors; D001241:Aspirin