Presence of a gene in the archaebacterium Methanococcus vannielii homologous to secY of eubacteria.
The nucleotide sequence of a gene located at the promoter-distal side of the 'spectinomycin-operon' homologue of the archaebacterium Methanococcus vannielii was determined. Its derived amino acid sequence displayed 20% (identical positions) or 52% (including conservative exchanges) similarity, respectively, to SECY from E coli. An alignment of the Methanococcus SECY with eubacterial SECY sequences showed the existence of 10 membrane-associated primary structure domains in equivalent positions. The 5' and 3' ends of the secY transcript were mapped and the gene was expressed in the T7 promoter/polymerase system in E col. The temperature-sensitive growth of the E coli mutant IQ292 which harbours a secYts mutation could be complemented by the secY gene from Methanococcus. This indicates that a protein integral to an archaebacterial ether-lipid membrane can be inserted into a eubacterial phospholipid membrane without apparent loss of function.
Auer
J
J
Lehrstuhl für Mikrobiologie, Universität München, Germany.
Spicker
G
G
Böck
A
A
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics; D001419:Bacteria / Q000235:genetics*; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics; D017017:Methanococcus / Q000235:genetics*; D016982:Micrococcus luteus / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009174:Mycoplasma / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D001426:Bacterial Proteins; D004269:DNA, Bacterial