Distribution of m-IBG in nude mice bearing LAN-5 neuroblastoma xenografts and in vitro cell culture.
BACKGROUND
Neuroblastoma is the commonest extra-cranial solid tumour of childhood and has a poor clinical outcome in patients with disseminated disease. Animal xenografts of this tumour offer a useful method of studying new diagnostic and therapeutic strategies for the tumour, prior to consideration of clinical trials.


METHODS
131I m-IBG was injected into nude mice bearing xenografts of the human neuroblastoma line LAN 5 in several sites. Animals were sacrificed at 24 (n = 6) and 48 hours (n = 5) and the biodistribution of the agent as well as uptake into the xenografted tumours was determined in a gamma well counter. In-vitro uptake of m-IBG into suspensions of LAN 5 cells was also determined in order to confirm the animal studies.


RESULTS
There was no preferential accumulation of m-IBG in the xenografted tumours in any of the sites considered. Similarly, the in-vitro uptake of m-IBG showed the typical Michaelis-Menten kinetics of simple diffusion with no evidence of active uptake.


CONCLUSIONS
The human neuroblastoma line LAN 5 failed to enrich m-IBG in either the xenograft or in-vitro situation. This may be related to the poor degree of differentiation of the tumour as evidenced by its unusual ease of growth in the sub-cutaneous site.
Van der Wall
H
H
Department of Nuclear Medicine, Prince of Wales Hospital, Sydney, Australia.
Durbidge
M
M
White
L
L
Taylor
D
D
Murray
I P
IP
D019797:3-Iodobenzylguanidine; D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D006801:Humans; D007457:Iodine Radioisotopes; D007462:Iodobenzenes / Q000493:pharmacokinetics*; D007700:Kinetics; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009447:Neuroblastoma / Q000378:metabolism*; D013997:Time Factors; D014018:Tissue Distribution; D014183:Transplantation, Heterologous
D007457:Iodine Radioisotopes; D007462:Iodobenzenes; D019797:3-Iodobenzylguanidine