[The identification of the membrane proteins of human and animal viruses].
The review deals with the methods of identification of virus-specific proteins on virion and infected cell surface. The isotopic labeling of membrane proteins, their extraction by proteolytic enzymes and selective solubilization by detergents are considered. The plasmatic membrane isolation by centrifugation and by means of microcarriers is described. The methods of membrane protein localization using monoclonal antibodies and radioimmunoprecipitation are presented.
Ustin
A V
AV
Kolontsov
A A
AA
Makarov
V V
VV
D000818:Animals; D003902:Detergents / Q000494:pharmacology; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000187:drug effects / Q000302:isolation & purification; D010447:Peptide Hydrolases / Q000494:pharmacology; D012995:Solubility; D014759:Viral Envelope Proteins / Q000032:analysis* / Q000187:drug effects / Q000302:isolation & purification; D014771:Virion / Q000737:chemistry / Q000187:drug effects; D014773:Virology / Q000379:methods
D003902:Detergents; D008565:Membrane Proteins; D014759:Viral Envelope Proteins; D010447:Peptide Hydrolases
Identifikatsiia membrannykh belkov virusov cheloveka i zhivotnykh.