[Latex test used for diagnosis of dysentery caused by Shigella sonnei].
The aim of this study was to evaluate the usefulness of latex reagent coated with immunoglobulins specific for antigens of phase I and II of S. sonnei for detection of these antigens in primary, mixed bacterial cultures. The study was performed on 919 fecal samples from individuals with clinical symptoms of dysentery, convalescents and from contact individuals. Material used for the test was bacterial suspension collected from McConkey or SS agars and a culture from selenite F broth heated at 100C. The results of the latex test were compared with the results of isolation of S. sonnei from the same cultures. S. sonnei was isolated from feces of 140 individuals (15.2%), while the latex test was positive in 215 cases (23.4%). The highest testing effectiveness , significantly higher than when isolation of pathogen was performed, was obtained only when 18-20 hr culture on Selenite F medium was used for latex test. The correlation between efficacy of testing for S. sonnei and phosphate content of Selenite F and a mode of its preparation was found. The latex test allows to eliminate from further bacteriological studies cultures free of S. sonnei thus it gives measurable economical profits and it shortens significantly time period of bacteriological examination.
Kałuzewski
S
S
Zakład Bakteriologii PZH w Warszawie.
Jagielski
M
M
D000942:Antigens, Bacterial / Q000032:analysis; D003470:Culture Media; D004405:Dysentery, Bacillary / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006801:Humans; D066298:In Vitro Techniques; D007841:Latex Fixation Tests / Q000379:methods; D011044:Poland; D012764:Shigella sonnei / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*
D000942:Antigens, Bacterial; D003470:Culture Media
Test lateksowy w zastosowaniu do diagnostyki czerwonki wywołanej przez S. sonnei.