Technetium-labelled autologous lymphocytes: clinical protocol for radiolabelling using a high concentration and low volume of 99Tcm-exametazime.
Little clinical work has been performed using technetium-labelled lymphocytes due to the difficulty of effecting an imaging dose of technetium on the small number of lymphocytes available. We have successfully labelled lymphocytes using a high concentration and low volume of 99Tcm-exametazime. In eight readings from three patients and two volunteers, the labelling efficiency was 27% (4 S.D.). The radiolabel was stable in plasma in vitro up to 4 h. Viability of the lymphocytes as determined by trypan blue exclusion was greater than 95%.
Sampson
C B
CB
Department of Nuclear Medicine, Addenbrooke's Hospital, Cambridge, UK.
Goffin
E
E
D001800:Blood Specimen Collection / Q000379:methods; D002074:Butanones; D002470:Cell Survival; D004355:Drug Stability; D006801:Humans; D066298:In Vitro Techniques; D007553:Isotope Labeling / Q000379:methods*; D007958:Leukocyte Count; D008214:Lymphocytes; D015609:Organotechnetium Compounds; D019690:Technetium Tc 99m Exametazime
D002074:Butanones; D015609:Organotechnetium Compounds; D019690:Technetium Tc 99m Exametazime