title: Early changes of alpha B-crystallin mRNA in rat skeletal muscle to mechanical tension and denervation.
abstract: alpha B-Crystallin specifically decreases in atrophied rat soleus muscle with hindlimb suspension (HS). alpha B-Crystallin cDNA was cloned from rat heart cDNA library using oligonucleotide probe, and its complete coding and partial non-coding regions were sequenced. Northern blot analysis revealed that alpha B-crystallin mRNA in slow muscle decreases at 36 hour after HS but recovered at 24 hour after HS stopped. Denervation decreased the expression of alpha B-crystallin mRNA in slow muscle but increased it in fast muscles, which hardly expressed in normal condition. Passive tension increased the expression of alpha B-crystallin mRNA in both muscle types. Based upon these Northern blot analysis of alpha B-crystallin, nerve innervation and external load on muscle are essential regulatory factors on the expression of the mRNA of alpha B-crystallin in rat skeletal muscle.
authors.affiliation: Department of Sports Sciences, College of Arts & Sciences, University of Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Atomi
authors.forename: S
authors.initials: S
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Nishida
mesh_terms: D000199:Actins / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001284:Atrophy; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003459:Crystallins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D006321:Heart / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D009121:Muscle Denervation; D009132:Muscles / Q000294:innervation / Q000473:pathology / Q000502:physiology*; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000199:Actins; D003459:Crystallins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D004247:DNA