Combined effects of Zn(2+)-chlorhexidine and Zn(2+)-cetylpyridinium chloride on caries incidence in partially desalivated rats.
The effects of Zn2+ combined with either chlorhexidine or cetylpyridinium chloride (CPC) on caries incidence in partially desalivated rats were investigated. Seven groups of 12 animals each received topical applications for 20 s with a saturated swab (0.2 ml) of the following aqueous solutions twice daily on weekdays (10 a.m. and 3 p.m.) and once daily during weekends (12 a.m.) for 5 wk: deionized water (placebo); 40 mM zinc acetate; 2.2 mM chlorhexidine diacetate; 4.4 mM CPC; 40 mM zinc acetate and 2.2 mM chlorhexidine diacetate; 40 mM zinc acetate and 4.4 mM CPC; and 20 mM NaF (positive control). Coronal caries was scored by the method of Keyes. All treatments except CPC alone resulted in significantly (P less than 0.05, ANOVA) less smooth-surface caries than did the placebo. NaF treatment resulted in significantly less smooth-surface caries than did Zn2+, chlorhexidine, CPC, and Zn(2+)-CPC. The inclusion of zinc ions did not significantly increase the caries-inhibitory efficacy of chlorhexidine (CH). The combination of Zn(2+)-CPC decreased smooth-surface scores significantly more than did CPC alone. Significant differences in sulcal-surface caries were not observed among the groups. Zn(2+)-CPC suppressed the Streptococcus sobrinus counts significantly more than did the separate agents. Animals treated with Zn(2+)-CH harbored the lowest populations of S. sobrinus.
Giertsen
E
E
Department of Dental Research, University of Rochester, New York.
Bowen
W H
WH
Pearson
S K
SK
D000085:Acetates / Q000008:administration & dosage / Q000627:therapeutic use*; D000287:Administration, Topical; D000818:Animals; D002594:Cetylpyridinium / Q000008:administration & dosage / Q000627:therapeutic use*; D002710:Chlorhexidine / Q000008:administration & dosage / Q000627:therapeutic use*; D003731:Dental Caries / Q000382:microbiology / Q000503:physiopathology / Q000517:prevention & control*; D004338:Drug Combinations; D005260:Female; D015994:Incidence; D008297:Male; D010919:Placebos; D051381:Rats; D011919:Rats, Inbred Strains; D012463:Saliva / Q000502:physiology; D012969:Sodium Fluoride / Q000008:administration & dosage / Q000627:therapeutic use; D013295:Streptococcus mutans / Q000187:drug effects / Q000302:isolation & purification; D014987:Xerostomia / Q000503:physiopathology*; D015032:Zinc / Q000008:administration & dosage / Q000627:therapeutic use*; D019345:Zinc Acetate
D000085:Acetates; D004338:Drug Combinations; D010919:Placebos; C072336:cetylpyridinium chloride, zinc acetate drug combination; C072338:chlorhexidine, zinc acetate drug combination; D012969:Sodium Fluoride; D002594:Cetylpyridinium; D019345:Zinc Acetate; D015032:Zinc; D002710:Chlorhexidine