title: 31P MRS of myocardial inorganic phosphate using radiofrequency gradient echoes.
abstract: Determination of the chemical shift and integral of the myocardial intracellular inorganic phosphate (Pi) resonance by 31P magnetic resonance spectroscopy (MRS) is often precluded due to a large overlapping signal from 2,3-diphosphoglycerate (2,3-DPG) from chamber and myocardial blood. This report demonstrates the use of radiofrequency (RF) magnetic field gradient echoes (RFGE) to eliminate signals from 2,3-DPG in flowing blood, while retaining signals from intracellular myocardial Pi, ATP, and phosphocreatine (PCr). The ECG-triggered 31P spectra were acquired from the myocardium of open chest pigs using a Philips Gyroscan 2-T magnetic resonance spectrometer. A 2.5-cm-diameter surface coil attached to the myocardium was used to provide the RF gradient as well as for excitation and detection of signals. Optimal performance of the RFGE pulse sequence was obtained when the RF gradient pulses were centered at peak diastole or peak systole. Under these conditions, 2,3-DPG signals were completely suppressed, and sensitivity was usually sufficient to allow detection of a well-resolved Pi signal. Myocardial pH determined from RFGE experiments was 7.16 +/- 0.10, and the ratio of the integrals of the Pi and ATP resonances (Pi/ATP) was 0.24. The mean signal-to-noise ratio (S/N) for PCr in control spectra acquired in 4 min was 19/1, while the mean S/N for PCr in RFGE-edited spectra acquired in 15 min was 11/1, demonstrating that the present implementation of the RFGE method results in significant loss in sensitivity. These experiments demonstrate that RFGE-editing allows accurate determination of the chemical shift and integral of the Pi resonance in blood-perfused myocardium in situ.
authors.affiliation: Magnetic Resonance Unit, Veterans Administration Medical Center, San Francisco.
authors.forename: J R
authors.initials: JR
authors.lastname: Gober
authors.forename: G G
authors.initials: GG
authors.lastname: Schwartz
authors.forename: S
authors.initials: S
authors.lastname: Schaefer
authors.forename: B M
authors.initials: BM
authors.lastname: Massie
authors.forename: G B
authors.initials: GB
authors.lastname: Matson
authors.forename: M W
authors.initials: MW
authors.lastname: Weiner
authors.forename: G S
authors.initials: GS
authors.lastname: Karczmar
mesh_terms: D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D004163:Diphosphoglyceric Acids / Q000097:blood; D004562:Electrocardiography; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism*; D010710:Phosphates / Q000032:analysis*; D010725:Phosphocreatine / Q000032:analysis; D011846:Radio Waves; D013552:Swine
chemical_list: D004163:Diphosphoglyceric Acids; D010710:Phosphates; D010725:Phosphocreatine; D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate