title: Synthesis and evaluation of radioiodinated cholesteryl ethers as lipoprotein probes.
abstract: Two novel cholesteryl ether derivatives were synthesized and radioiodinated: (1) [125I]cholesteryl m-iodobenzyl ether (125I-CIBE) and (2) [125I]cholesteryl 12-(m-iodophenyl)dodecyl ether (125I-CIDE). These radioiodinated ethers were incorporated into low-density lipoprotein (LDL) by incubating the compounds (solubilized in saline with Tween-20) with isolated LDL or with whole plasma. Such LDL preparations were taken up by cultured fibroblasts in a receptor-dependent manner similar to that of radioiodinated LDL. Upon injection into guinea pigs, 125I-CIBE-labeled guinea pig LDL cleared from the plasma similarly to radioiodinated guinea pig LDL. The primary sites of 125I-CIBE uptake were the adrenal and the liver, and the compound was stable to both hydrolysis and deiodination over 24 h. In summary, 125I-CIBE and 125I-CIDE, like previously described tritiated cholesteryl ethers, appear to be potentially useful tracers of cholesteryl ester uptake. Moreover, these radioiodinated probes have the advantage of being more easily quantitated in tissue samples as well as being detectable by noninvasive scintigraphic imaging.
authors.affiliation: Department of Pharmacology, University of Michigan, Ann Arbor.
authors.forename: L E
authors.initials: LE
authors.lastname: DeForge
authors.forename: M K
authors.initials: MK
authors.lastname: Ruyan
authors.forename: S W
authors.initials: SW
authors.lastname: Schwendner
authors.forename: R S
authors.initials: RS
authors.lastname: Newton
authors.forename: R E
authors.initials: RE
authors.lastname: Counsell
mesh_terms: D000818:Animals; D001593:Benzyl Compounds / Q000097:blood / Q000493:pharmacokinetics; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000097:blood / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D004355:Drug Stability; D004987:Ethers / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D006168:Guinea Pigs; D007457:Iodine Radioisotopes / Q000737:chemistry*; D008074:Lipoproteins / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D014018:Tissue Distribution
chemical_list: D001593:Benzyl Compounds; D004987:Ethers; D007457:Iodine Radioisotopes; D008074:Lipoproteins; D008077:Lipoproteins, LDL; C072484:cholesteryl-3-iodobenzyl ether; C072486:cholesteryl-12-(3-iodophenyl)dodecyl ether; D002784:Cholesterol