Relationships between phosphatidylcholine content, chitin synthesis, growth, and morphology of Aspergillus nidulans choC.
The phosphatidylcholine (PC) content of Aspergillus nidulans choC was varied by growing the auxotroph in medium containing various concentrations of choline chloride. Direct linear correlations were observed between PC content and in vivo chitin synthase activity, between in vivo chitin synthase activity and mean hyphal extension rate, and between mean hyphal extension rate and hyphal growth unit length; hyphal growth unit length is a measure of hyphal branching. Further, there was a correlation between PC content and colony radial growth rate. Thus, membrane composition is an important determinant of both hyphal (and colony) extension rate and mycelial morphology.
Binks
P R
PR
Department of Cell and Structural Biology, School of Biological Sciences, Manchester, U.K.
Robson
G D
GD
Goosey
M W
MW
Trinci
A P
AP
D000117:Acetylglucosamine / Q000378:metabolism; D001233:Aspergillus nidulans / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D002686:Chitin / Q000096:biosynthesis*; D002687:Chitin Synthase / Q000378:metabolism; D002794:Choline / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D010713:Phosphatidylcholines / Q000378:metabolism*
D010713:Phosphatidylcholines; D002686:Chitin; D002687:Chitin Synthase; D002794:Choline; D000117:Acetylglucosamine