Genetic quality control of laboratory animals used in aging studies.
Laboratory rodents provide a useful model for aging processes in humans. Various genetic "types" of stock are available, including outbred stocks and inbred strains and their derivatives. Inbred strains, which can be regarded as clones of genetically identical individuals, provide a powerful research tool for studies in many disciplines, including gerontology. However, some form of genetic quality control is essential to ensure that the strains are authentic. Single gene polymorphisms, particularly those detected by electrophoresis and immunological methods, provide a powerful tool for such quality control, though these methods are expensive and require considerable expertise. Methods based on several loci studied simultaneously include skin grafting and polyvalent alloantisera. These methods are often quick and technically relatively easy, but are less flexible than the single locus methods. Methods based on DNA restriction fragment length polymorphisms (RFLPs) fall into both categories depending on whether a single locus or a multilocus probe, such as the fingerprinting probes, is used. These DNA-based methods have many advantages, and are likely to be the methods of choice in the future.
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MRC Toxicology Unit, Carshalton, Surrey, UK.
D000375:Aging / Q000235:genetics* / Q000502:physiology; D000818:Animals; D000830:Animals, Laboratory / Q000235:genetics*; D011786:Quality Control