title: Transient down-regulation of c-myc protooncogene expression by the alkaloid nicotine in human promyelocytic HL-60 cells.
abstract: Human promyelocytic leukemia cells (HL-60) acquire monocytic biochemical characteristics when exposed to the alkaloid nicotine. We report herein that the nicotine-induced phenotype change is accompanied by the transient reduced expression of mRNA for the protooncogene c-myc. The suppressive effect of nicotine on c-myc mRNA levels is not reversed by the addition of ADP-ribosyl transferase inhibitor, 3-aminobenzamide.
authors.affiliation: Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595.
authors.forename: J A
authors.initials: JA
authors.lastname: Maturana
authors.forename: M
authors.initials: M
authors.lastname: Fraioli
authors.forename: J M
authors.initials: JM
authors.lastname: Wu
mesh_terms: D001549:Benzamides / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002461:Cell Line, Transformed / Q000187:drug effects; D015536:Down-Regulation; D004338:Drug Combinations; D004950:Esterases / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D016259:Genes, myc / Q000187:drug effects*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics*; D009538:Nicotine / Q000494:pharmacology*; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D009693:Nucleic Acid Hybridization
chemical_list: D001549:Benzamides; D004338:Drug Combinations; D009580:Nitroblue Tetrazolium; D009538:Nicotine; C025160:3-aminobenzamide; D004950:Esterases