Nerve cables formed in silicone chambers reconstitute a perineurial but not a vascular endoneurial permeability barrier.
The passage of molecules into the endoneurial environment of the axons of normal peripheral nerve is regulated by two permeability barriers, the perineurial-nerve barrier and the endoneurial blood-nerve barrier. These barriers exist because of the presence of tight junctions between adjacent perineurial cells and adjacent endothelial cells. In the present study we investigated whether permeability barriers form in nerve cables, which develop inside silicone chambers. The sciatic nerves of adult rats were cut, and the proximal and distal ends sutured into opposite ends of silicone chambers that were filled with dialyzed plasma. The presence of barriers was determined with the tracer horseradish peroxidase (HRP), which was injected intravenously and detected histochemically in tissues by light and electron microscopy. At four weeks, a regenerated nerve cable extended across the 10 mm length of each chamber. However, no permeability barriers were present since the reaction product for HRP was visible throughout the cable. At twenty-six weeks, all the axons in cables were gathered into minifascicles. Each minifascicle of axons was surrounded by perineurial cells. Blood vessels were excluded from the minifascicles by the perineurial cells and the vessels were permeable to HRP, thus indicating that their endothelial cells had not formed tight junctions. Despite the leakage of HRP from the excluded vessels, the tracer did not reach the axons because the perineurial cells encircling the minifascicles developed tight junctions. In some animals, the chambers were removed at four weeks to determine whether the chamber influenced barrier development. This manipulation had no effect since cables, with or without chambers, exhibited similar findings at twenty-six weeks. Our results indicate that nerve cables regenerate a perineurial but not an endoneurial permeability barrier. We conclude that axons in long-term cables are protected by only a perineurial permeability barrier.
Azzam
N A
NA
Laboratory of Neural Control, NINDS, NIH, Bethesda, Maryland 20892.
Zalewski
A A
AA
Williams
L R
LR
Azzam
R N
RN
D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D006735:Horseradish Peroxidase; D008854:Microscopy, Electron; D009024:Morphogenesis; D009412:Nerve Fibers / Q000378:metabolism / Q000648:ultrastructure*; D009416:Nerve Regeneration; D010539:Permeability; D019736:Prostheses and Implants; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000293:injuries / Q000502:physiology*; D012828:Silicones; D013997:Time Factors
D012828:Silicones; D006735:Horseradish Peroxidase