[The isolation and characteristics of hybridomas producing monoclonal antibodies to the structural proteins of the Vnukovo-32 strain of the rabies virus].
A series of hybridomas secreting monoclonal antibodies (MCA) to glycoprotein and nucleocapsid proteins of rabies virus strain Vnukovo-32 was selected as a result of fusion of splenocytes from immune BALB/c mice with cells of myeloma line Sp2/OAq14, screening and cloning by limiting dilution methods in semi-liquid agar. Four hybridomas secreted MCA to glycoprotein in high titres (5.0 x 10(5)-2.2 x 10(6)) and had marked virus-neutralizing and therapeutic properties. Eight hybridomas produced MCA to the nucleocapsid complex: five hybridomas secreted MCA of the G class in high titres (2.4 x 10(5)-1.6 x 10(6)) and three hybridomas secreted MCA of the M class in low titres.
Gribencha
S V
SV
Vasilenko
O V
OV
Furalev
V A
VA
Kliushnik
S Iu
SIu
Kuz'mitskaia
T M
TM
Sveshinkov
P G
PG
Barinskiĭ
I F
IF
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis*; D002213:Capsid / Q000276:immunology; D006825:Hybridomas / Q000276:immunology*; D006863:Hydrogen-Ion Concentration; D007114:Immunization / Q000379:methods; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D011820:Rabies virus / Q000276:immunology*; D014758:Viral Core Proteins / Q000276:immunology; D014760:Viral Fusion Proteins / Q000276:immunology; D015678:Viral Structural Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014758:Viral Core Proteins; D014760:Viral Fusion Proteins; D015678:Viral Structural Proteins
Poluchenie i kharakteristika gibridom, produtsiruiushchikh monoklonal'nye antitela k strukturnym belkam virusa beshenstva, shtamm Vnukovo-32.