Interlaboratory evaluation of three culture media for postimplantation rodent embryos.
The first aim of the study was to compare the ability of rat serum, human serum, and a mixture of human and rat serum (4:1) to support in vitro development of rodent postimplantation embryos. The comparison was made in three laboratories using rat embryos and in one laboratory using mouse embryos. Batches of sera, initial developmental stage, duration of culture, and endpoints were identical in the laboratories. The second aim of the study was to evaluate if other variables that could not be standardized would significantly influence the results of the laboratories. No reproducible difference was observed among the culture media or among the laboratories except that growth and differentiation were slower in the laboratory using mouse embryos. Further experiments are needed to exclude small differences in performance of the media.
Van Maele-Fabry
G
G
Laboratory of Developmental Genetics, U.C.L., Louvain-la-Neuve, Belgium.
Picard
J J
JJ
Attenon
P
P
Berthet
P
P
Delhaise
F
F
Govers
M J
MJ
Peters
P W
PW
Piersma
A H
AH
Schmid
B P
BP
Stadler
J
J
D000818:Animals; D000013:Congenital Abnormalities / Q000473:pathology; D003470:Culture Media; D046508:Culture Techniques; D004622:Embryo, Mammalian / Q000502:physiology*; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D051379:Mice; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains
D003470:Culture Media