The analysis of thiocarbamides by gas chromatography/negative-ion chemical-ionization mass spectrometry.
Thiocarbamides were converted to their di-N-pentafluorobenzyl (PFB) derivatives and analysed by gas chromatography/negative-ion chemical-ionization mass spectrometry with methane as reagent gas. The PFB derivatives of the 2-thiouracils gave mass spectra in which the ion current was carried largely by an ion arising from [M-PFB]-. The derivative was used in the determination of the uptake and metabolism of thiocarbamides by cultures of melanoma cells.
Watson
D G
DG
Department of Pharmacy, University of Strathclyde, Glasgow, UK.
Bates
C D
CD
Skellern
G G
GG
Mairs
R
R
Martin
S
S
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008545:Melanoma / Q000378:metabolism*; D008713:Methimazole / Q000493:pharmacokinetics; D008779:Methylthiouracil / Q000493:pharmacokinetics*; D011441:Propylthiouracil / Q000493:pharmacokinetics*; D013889:Thiouracil / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D014407:Tumor Cells, Cultured
D008713:Methimazole; D013889:Thiouracil; D011441:Propylthiouracil; C019269:benzylthiouracil; D008779:Methylthiouracil