title: Chromatographic separation of mixed peptides from amino acids in biological digests with volatile buffers.
abstract: Two chromatographic methods, capable of separating mixed peptides from contaminating amino acids in biological digests, are described. Both methods involve separation on copper-Chelex resin, but each uses a different set of elution buffers. When separation method 1 was applied to a commercially available proteolytic digest of casein, the free amino acid content was reduced from 26.0% to 0.5%. With an enzymic digest of 14C-labelled proteins derived from plant biomass, separation method 2 decreased the contaminating free amino acids from 20.3% to 1.9%. Since the separated peptides are eluted with volatile buffers, they are suitable as substrates for biological experiments.
authors.affiliation: Department of Biochemistry, University College of Wales, UK.
authors.forename: I P
authors.initials: IP
authors.lastname: Armstead
authors.forename: J R
authors.initials: JR
authors.lastname: Ling
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D002021:Buffers; D002364:Caseins / Q000032:analysis*; D002845:Chromatography / Q000379:methods*; D001467:Hordeum / Q000737:chemistry*; D010455:Peptides / Q000032:analysis*
chemical_list: D000596:Amino Acids; D002021:Buffers; D002364:Caseins; D010455:Peptides