Glutathione and related enzyme activity in the 11-day rat embryo, placenta and perinatal rat liver.
Glutathione reductase (EC 1.6.4.2; GSSG-R), glutathione peroxidase (EC.1.11.1.9; GSHpx) and glutathione transferase (EC 2.5.1.18; GST) enzymatic activities and glutathione status were investigated in 11-day embryos and the yolk sac, placenta and perinatal liver in rats. It is observed that: (a) levels of GSSG differ between the embryo (lower) and yolk sac (higher); (b) GSH concentrations increased significantly in fetal livers with respect to the days of gestation; in contrast, GSSG hepatic concentrations showed a significant rise with respect to time only during lactation; (c) the specific enzymatic activity of both GSHpx and GSSG-R were higher in the visceral yolk sac than in the embryo; (d) hepatic GSSG-R activity increased significantly during gestation. In addition, hepatic GSHpx and GST activities showed statistically significant increases over the period studied.
Serafini
M T
MT
Department d'Enginyeria Química i Bioquímica, Universitat de Barcelona, Tarragona, España.
Arola
L
L
Romeu
A
A
D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000032:analysis; D005980:Glutathione Reductase / Q000032:analysis*; D005982:Glutathione Transferase / Q000032:analysis*; D008099:Liver / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D015017:Yolk Sac / Q000378:metabolism
D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione