title: Determination of zopiclone enantiomers in plasma by liquid chromatography using a chiral cellulose carbamate column.
abstract: The enantiomers of zopiclone were determined in human plasma using a sequential achiral-chiral liquid chromatographic method. Zopiclone was separated from the biological matrix and quantified on an achiral silica column. The limit of detection was 5 ng/ml. The eluent fraction containing zopiclone was collected, evaporated, reconstituted with the mobile phase and injected onto a chiral cellulose carbamate column where the enantiomeric ratio was calculated. This validated method, applied to a pilot study, suggests that pharmacokinetics of zopiclone is stereoselective.
authors.affiliation: Hôpital Pitié Salpétrière, Service Pharmacie, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Fernandez
authors.forename: B
authors.initials: B
authors.lastname: Baune
authors.forename: F
authors.initials: F
authors.lastname: Gimenez
authors.forename: A
authors.initials: A
authors.lastname: Thuillier
authors.forename: R
authors.initials: R
authors.lastname: Farinotti
mesh_terms: D053961:Azabicyclo Compounds; D002219:Carbamates; D002482:Cellulose; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D006801:Humans; D006993:Hypnotics and Sedatives / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D010879:Piperazines / Q000097:blood* / Q000493:pharmacokinetics; D013237:Stereoisomerism
chemical_list: D053961:Azabicyclo Compounds; D002219:Carbamates; D006993:Hypnotics and Sedatives; D010879:Piperazines; C515050:zopiclone; D002482:Cellulose