Effects of prostaglandin E2 and F2 alpha on peri-implantation development of mouse embryos in vitro.
The effects of exogenous prostaglandin (PG) E2 and F2 alpha on the morphology and lactate dehydrogenase (LDH) activities of pre-implantation mouse embryos in vitro were studied. A 24-hour exposure from 0.01 to 10 micrograms/ml of PGE2 at the 4-cell or morula stages had no effect on the morphology of embryos during the 144 hours in culture. Exposure to 10 micrograms/ml PGE2 at the blastocyst stage accelerated and enhanced spreading of the trophoblast in vitro. Embryos treated at 0.01 to 10 micrograms/ml PGE2 at various stages all showed a more rapid decline in LDH activity from morula to blastocysts. Treatment with 50 or 100 micrograms/ml PGE2 led to abnormal morphology of embryos in vitro. In contrast, continuous treatment with 0.01 to 100 micrograms/ml PGF2 alpha from 4-cell to early post-implantation (day 8) had no effect on the morphology of embryos, although breakdown of LDH was again accelerated. These results suggest that the peak of PGE2 secretion on day 4 of pregnancy in mice may enhance trophoblastic outgrowth, and the lower levels of PGE2 and PGF2 alpha secreted earlier in pregnancy modulate the development of pre-implantation mouse embryos.
Chan
S Y
SY
Department of Zoology, University of Hong Kong.
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D015237:Dinoprost / Q000494:pharmacology*; D015232:Dinoprostone / Q000494:pharmacology*; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D009028:Morula / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D009924:Organ Culture Techniques; D010784:Photomicrography; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014327:Trophoblasts / Q000187:drug effects
D015237:Dinoprost; D007770:L-Lactate Dehydrogenase; D015232:Dinoprostone