Radioimmunodetection of human colon cancer in nude mice by a new monoclonal antibody A7 against human colorectal cancer.
The in vivo localization of a monoclonal antibody A7 against a human colorectal cancer was studied in nude mice bearing human solid carcinomas, to evaluate potential applications of this antibody for radioimmunodetection of cancer. The tissue distribution of 125I-labeled A7 MoAb at 3 days after i.v. injection into mice bearing five different kinds of human solid tumors revealed a high uptake ratio by colon cancer, mammary cancer, and glioblastoma. In contrast, the uptake ratio by murine colorectal cancer (Colon-38) was extremely low. In immunoscintigraphic studies, HCT-15, one of the human colon cancer, was clearly visualized with 111In-DTPA-A7 MoAb. Glioblastoma was also imaged with the same extent. These results suggest that A7 MoAb would be applicable to the in vivo radioimmunodetection of colon- and mammary-cancer, and of glioblastoma.
Kojima
S
S
Research Institute for Biosciences, Science University of Tokyo, Chiba, Japan.
Shimura
N
N
Kubodera
A
A
Kubota
K
K
Takahashi
T
T
Oyamada
H
H
D000230:Adenocarcinoma / Q000000981:diagnostic imaging; D000818:Animals; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging*; D015179:Colorectal Neoplasms / Q000276:immunology*; D005260:Female; D005729:Ganglioneuroma / Q000000981:diagnostic imaging; D006801:Humans; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016719:Radioimmunodetection; D014018:Tissue Distribution
D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes