19-Nordeoxycorticosterone synthesis by rat kidney inner medullary collecting duct cells.
19-Nordeoxycorticosterone (19-nor-Doc), a potent mineralocorticoid, was found to be synthesized by the isolated rat kidney perfused by an adrenal precursor (19-oxo-Doc). To determine if this bioconversion is a function of renal tubular cells, various adrenal precursors of 19-nor-Doc were added separately to rat kidney inner medullary collecting duct cells culture media at a concentration of 10 nM. While 4.6% +/- 1.0% of 19-oxo-Doc (n = 3) and 14.4% +/- 1.4% of 19-oic-Doc (n = 3) were converted to 19-nor-Doc after 24 hours of incubation, Doc, and 19-OH-Doc were not converted. This represents further evidence that Doc has to be metabolized to 19-oxo-Doc or 19-oic-Doc (19-carboxy-Doc) before it can be converted by the kidney inner medullary collecting duct cells to 19-nor-Doc.
Azar
S T
ST
Evans Medical Foundation, Department of Clinical Research and Medicine, University Hospital, Boston, Massachusetts.
Melby
J C
JC
Wilson
T E
TE
Nichols
R
R
Schwartz
J H
JH
D000704:Analysis of Variance; D000818:Animals; D002478:Cells, Cultured; D003900:Desoxycorticosterone / Q000031:analogs & derivatives* / Q000096:biosynthesis; D007679:Kidney Medulla / Q000166:cytology / Q000378:metabolism*; D007685:Kidney Tubules, Collecting / Q000166:cytology / Q000378:metabolism*; D051381:Rats
D003900:Desoxycorticosterone; C019026:19-nordeoxycorticosterone