Detection of Ki-ras proto-oncogene protein by a specific monoclonal antibody.
We have used a commercially available mouse monoclonal antibody and shown it to bind specifically to cellular Ki-ras p21 proteins and not to cellular N- and Ha-ras p21 proteins. In conjunction with electrophoresis and Western blotting, this antibody can be used, with further detailing, to assess levels of the cellular Ki-ras p21 against a background of total p21s.
Palejawala
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S
Department of Obstetrics and Gynecology, UMDNJ-New Jersey Medical School, Newark 07103.
Goldsmith
L T
LT
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D010053:Ovary / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D016283:Proto-Oncogene Proteins p21(ras) / Q000032:analysis* / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000378:metabolism
D000911:Antibodies, Monoclonal; D016283:Proto-Oncogene Proteins p21(ras)