[Genotyping the bovine kappa-casein locus using polymerase chain reaction].
Polymerase chain reaction (PCR) was used for detecting kappa-casein (kappa-casein) genotype in the synthetic breed (Jersey x Black and White x Holstein-Friesian) dairy cattle. The amplified 228 bp fragment includes a region, where relevant mutations lead to both the appearance of different kappa-casein alleles associated with amino acid substitutions and the appearance of new TaqI and HindIII restriction sites in ae-casein B gene. The specificity of the kappa-casein gene fragment amplification was supported by restriction analysis and Southern blot hybridization. Digestion of amplified fragment with endonucleases PstI, HindIII and/or TaqI allows detection of AA-, AB- and BB genotypes of kappa-casein. A total of 32 animals with known (18 samples) and unknown (14 samples) kappa-casein phenotypes were tested using PCR and blot hybridization. In all known cases the detected genotype confirmed the phenotype. Frequencies of the B allele and of the AB genotype in the breeding population are rather high (53.1 +/- 8.8 and 43.7%, respectively). The possibility of effective use of the PCR analysis for genotyping kappa-casein locus in bulls and their offspring has been shown. The advantages of the PCR method in large breeding programs and linkage analysis have been discussed.
Sulimova
G E
GE
Shaĭkhaev
G O
GO
Berberov
E M
EM
Markarian
A Iu
AIu
Kandalova
L G
LG
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002364:Caseins / Q000235:genetics*; D002417:Cattle; D005838:Genotype; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
D002364:Caseins
Genotipirovanie lokusa kappa-kazeina u krupnogo rogatogo skota s pomoshch'iu polimeraznoĭ tsepnoĭ reaktsii.