Biochemical and immunohistochemical analysis of orotic acid-induced fatty liver.
Orotic acid-induced fatty livers were examined by biochemical and immunohistochemical approaches. Lipid peroxide levels by the thiobarbituric acid method and glutathione-peroxidase (GSH-PO) activity in the liver homogenates from orotic administered rats were similar to those of controls. Immunohistochemical localization of GSH-PO in orotic acid-induced fatty liver was mainly observed in the portal zone of the hepatic lobules. This staining pattern of GSH-PO was similar to that of the controls. No remarkable changes in GSH-PO staining patterns were detected in orotic acid-induced fatty liver. Our data strongly suggested that no lipid peroxidation is actively involved in the genesis of fatty liver due to the administration of orotic acid, and GSH-PO a protective enzyme against lipid peroxidation, was not inhibited by orotic acid-induced fatty liver.
Murakoshi
M
M
Department of Pathology, School of Medicine, Tokai University.
Suemizu
H
H
Takekoshi
S
S
Misawa
M
M
Watanabe
K
K
D000818:Animals; D005234:Fatty Liver / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D005979:Glutathione Peroxidase / Q000378:metabolism; D007150:Immunohistochemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D009963:Orotic Acid / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains
D008054:Lipid Peroxides; D009963:Orotic Acid; D005979:Glutathione Peroxidase