Sequence analysis of a mitochondrial DNA fragment isolated from cultured cells of carrot cytoplasmic male-sterile strain.
2.0 kb Hind III fragment isolated from cytoplasmic male-sterile carrot mitochondria, designated PKT5, was hybridized to ORF13 which is the coding region of a unique polypeptide in maize CMS (Dewey et al., 1986). Sequence analysis indicated that PKT5 is consisted of 3 domains. Domain 1 was identical to the 5'-flanking region of atp6 in maize CMS-TURF2H3 sequence (Dewey et al., 1986). Domain 2 contained a novel ORF encoding 72 amino acids, which was extremely homologous to the amino-terminal 67 amino acids of the unique ORF13 in maize CMS. Domain 3 except an amino acid change (Ile87 = ATT for Asn87 = AAT), was identical to ORF25 polypeptide in maize CMS. Connective sequences of these 3 domains were also highly homologous to the maize CMS-TURF2H3 sequence. Out of 7 recombination points in maize CMS-TURF2H3 sequence, at least 4 points were conserved in PKT5 sequence.
Kanzaki
H
H
Institute of Genetic Ecology, Tohoku University, Sendai, Japan.
Takeda
M
M
Kameya
T
T
D001483:Base Sequence; D015139:Blotting, Southern; D002478:Cells, Cultured; D003593:Cytoplasm / Q000502:physiology; D004272:DNA, Mitochondrial / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010944:Plants; D012098:Reproduction / Q000235:genetics; D003313:Zea mays / Q000235:genetics
D004272:DNA, Mitochondrial