Characterization of a Cryptosporidium parvum sporozoite glycoprotein.
Polyclonal and monoclonal antibodies directed against Cryptosporidium oocysts or sporozoites were developed to identify and characterize sporozoite pellicle and apical complex antigens. A very large glycoprotein of Cryptosporidium sporozoites was identified by three monoclonal antibodies that also reacted with intracellular merozoites. The glycoprotein was also identified by polyclonal antibodies that were affinity-purified on nitrocellulose-bound recombinant proteins expressed by four lambda gtll genomic clones.
Petersen
C
C
Department of Medicine, San Francisco General Hospital, CA 94110.
Gut
J
J
Nelson
R G
RG
Leech
J H
JH
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D003458:Cryptosporidium / Q000737:chemistry* / Q000276:immunology; D005260:Female; D015698:Genomic Library; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins / Q000276:immunology / Q000302:isolation & purification*; D013170:Spores / Q000737:chemistry / Q000276:immunology
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D015800:Protozoan Proteins