The effect of interleukin-1 on venous endothelium--an ultrastructural study.
The effect of systemic interleukin-1 (IL-1) on venous endothelium in the presence and absence of stasis has been studied by scanning electron microscopy (SEM). Recombinant human IL-1 beta at a concentration of 1 micrograms/kg or saline was injected intravenously into rabbits and allowed to circulate for 0.5 or 4.0 h after which complete stasis was induced for 1 h in an isolated segment of each jugular vein. One vein segment was then excised and the contents examined macroscopically for thrombi, while the other segment was fixed for SEM examination. When examined by SEM the endothelium from rabbits injected with IL-1 beta was perturbed with increased surface microvilli, blebs and gaps at cell junctions when compared with saline controls. Fibrin deposition was also observed after IL-1 beta, as was the adherence of essentially non-activated platelets to intact endothelium. However, macroscopic thrombi were not formed in isolated vein segments. We conclude that although fibrin strands and platelets were deposited on the endothelium, IL-1 is not a sufficiently powerful procoagulant stimulus to lead to an occlusive thrombus in acute experiments.
Merton
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RE
National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire, UK.
Hockley
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D
Gray
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Poole
S
S
Thomas
D P
DP
D000818:Animals; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000648:ultrastructure; D006487:Hemostasis; D007375:Interleukin-1 / Q000494:pharmacology*; D008854:Microscopy, Electron; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D013927:Thrombosis / Q000473:pathology*
D007375:Interleukin-1; D011994:Recombinant Proteins