[Fluorescence label of alcohols and amines with pyrene-1-carbonyl fluoride].
Pyrene-1-carbonyl fluoride (PCF) was synthesized as a precolumn fluorescent labeling reagent for alcohols and amines for use in high performance liquid chromatography (HPLC). PCF reacted with primary and phenolic hydroxyl groups in dichloromethane at 100 degrees C for 30 min in the presence of 4-dimethylaminopyridine (DMAP) to give the corresponding fluorescent pyrene esters and also reacted with primary amines in acetonitrile at room temperature for 2 min in the presence of DMAP to give the amides. The PCF esters of corticosteroids were separated by normal-phase chromatography on a Cosmosil 5 SL column with hexane-ethyl acetate (6:5, v/v) and the PCF amides of primary amines were separated by reversed-phase chromatography on a TSK gel ODS-80 TM column with methanol-water (10:3, v/v). The detection limits (S/N = 3) of cortisone and 2-phenylethylamine were 600 fmol and 800 fmol for an injection volume of 10 microliters, respectively.
Fujino
H
H
Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Goya
S
S
D000438:Alcohols / Q000032:analysis; D000588:Amines / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D011721:Pyrenes / Q000138:chemical synthesis*
D000438:Alcohols; D000588:Amines; D005456:Fluorescent Dyes; D011721:Pyrenes; C073304:pyrene-1-carbonyl fluoride