Cadmium injury of cultured human vascular endothelial cells.
The effect of cadmium chloride (CdCl2) on cultured human vascular endothelial (HVE) cells and cultured human fibroblasts (HAIN-55 cells) was investigated. Umbilical vein-derived HVE cells were collected by enzymatic digestion with collagenase. At the concentration of 0-10 microM, Cd had hardly any effect on the cell viability of either cells. The viability of HVE cells decreased markedly at 100 microM, but not that of HAIN-55 cells. Morphologic examination by phase contrast microscopy revealed a more damaging effect of Cd on HVE cells than on HAIN-55 cells. These results suggest that Cd is more cytotoxic to HVE cells than HAIN-55 cells.
Kishimoto
T
T
Department of Environmental Medicine, Shimane Medical University.
Fukuzawa
Y
Y
Abe
M
M
Isobe
M
M
Hashimoto
M
M
Tada
M
M
D002104:Cadmium / Q000633:toxicity*; D019256:Cadmium Chloride; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002712:Chlorides / Q000633:toxicity*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans
D002712:Chlorides; D002104:Cadmium; D019256:Cadmium Chloride