A rapid PCR-based method to distinguish between fetal and maternal cells in chorionic biopsies using microsatellite polymorphisms.
In some chorionic villus biopsy (CVB) cases the fetal/maternal origin of the tissue obtained is uncertain. An approach which only requires small amounts of CVB tissue to establish its origin is described. Since it is only the samples typed as female that could be either fetal or maternal, a paternal X chromosome contribution is sought by using highly polymorphic X-linked microsatellites.
Noble
J S
JS
Yorkshire Regional DNA Laboratory, Leeds.
Taylor
G R
GR
Stewart
A D
AD
Mueller
R F
RF
Murday
V A
VA
D000483:Alleles; D015193:Chorionic Villi Sampling; D016172:DNA Fingerprinting; D015342:DNA Probes; D004276:DNA, Satellite / Q000235:genetics*; D005069:Evaluation Studies as Topic; D005220:Fathers; D005260:Female; D005333:Fetus; D005819:Genetic Markers; D006801:Humans; D009035:Mothers; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D011247:Pregnancy; D014960:X Chromosome
D015342:DNA Probes; D004276:DNA, Satellite; D005819:Genetic Markers