title: Purification of a nuclear protein (receptor binding factor-1) associated with the chromatin acceptor sites for the avian oviduct progesterone receptor.
abstract: The specific high affinity binding of the avian oviduct progesterone receptor (PR) to target cell nuclei and chromatin has been shown to involve DNA complexed with specific chromatin acceptor proteins. One of these chromatin acceptor proteins has been partially purified and found to be a small hydrophobic protein with a broad pI of 5.0-6.0 [Goldberger and Spelsberg (1988), Biochem. 27, 2103-2109]. Using western immunoblots with anti-RBF-1 polyclonal antibodies to monitor the purification, a 10 kD candidate acceptor protein, termed the Receptor Binding Factor-1 (RBF-1), has been purified to apparent homogeneity. RBF-1 has an amino acid composition consistent with a hydrophobic protein having an acidic pI and a unique N-terminal sequence. Two-dimensional polyacrylamide gel electrophoresis and high-performance capillary electrophoresis support the purity of a protein congruent to 10 kD in size, having an acidic pI, but with evidence of several differently charged isoforms. Phosphatase treatment provides evidence that charge heterogeneity may result from variable phosphorylation states. A role of this factor as a candidate "acceptor protein" in the chromatin acceptor sites for the avian oviduct PR is proposed.
authors.affiliation: SYVA Corp., Palo Alto, California 94303.
authors.forename: J
authors.initials: J
authors.lastname: Rejman
authors.forename: J
authors.initials: J
authors.lastname: Landers
authors.forename: A
authors.initials: A
authors.lastname: Goldberger
authors.forename: D J
authors.initials: DJ
authors.lastname: McCormick
authors.forename: B
authors.initials: B
authors.lastname: Gosse
authors.forename: T C
authors.initials: TC
authors.lastname: Spelsberg
mesh_terms: D000818:Animals; D001665:Binding Sites; D002645:Chickens; D002843:Chromatin / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D009687:Nuclear Proteins / Q000302:isolation & purification* / Q000378:metabolism; D010057:Oviducts / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D011980:Receptors, Progesterone