Determination of 6-chloro-3-(3-cyclopropyl 1,2,4-oxadiazol-5-yl)-5-methyl-imidazo less than 1,5-a greater than-quinoxalin-4(5h)-one in rat serum, urine and brain by solid-phase extraction and liquid chromatography.
A simple, rapid, and accurate liquid chromatographic method with ultraviolet detection and solid-phase extraction is described for the quantitation of 6-chloro-3-(3-cyclopropyl 1,2,4-oxadiazol-5-yl)-5-methyl-imidazo less than 1,5-a greater than-quinoxalin-4(5h)-one (I, U-80447) in rat serum, urine and brain. Linear calibration curves were obtained in the concentration ranges of 5 ng ml-1-20 micrograms ml-1 (serum), 20 ng ml-1-20 micrograms ml-1 (urine), and 50 ng g-1-200 micrograms g-1 (brain). Intra- and inter-assay precision and accuracy were all found to be less than 10% at the three concentrations evaluated. The absolute extraction recovery each from serum, urine and brain was greater than or equal to 90%. Application of this method to the quantitation of the title compound in rat serum and brain for a pharmacokinetic study is reported.
Zhong
W Z
WZ
Drug Metabolism Research, Upjohn Laboratories, Kalamazoo, MI 49001.
D000818:Animals; D001682:Biological Availability; D001923:Brain Chemistry; D001643:Bridged Bicyclo Compounds / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D008297:Male; D010069:Oxadiazoles / Q000032:analysis* / Q000097:blood / Q000652:urine; D011810:Quinoxalines / Q000032:analysis* / Q000097:blood / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains
D001643:Bridged Bicyclo Compounds; D010069:Oxadiazoles; D011810:Quinoxalines; C074865:U 80447