The effects of 1,2-dibromo-3-chloropropane (DBCP) on general toxicity and gonadotoxicity in rats.
This study was set to determine if there is a correlation between the general toxic effect and the gonadotoxic effect of DBCP on male rats. Groups of male rats were injected with a single dose of DBCP (50 mg kg-1) dissolved in dimethylsulfoxide (DMSO). Twenty four hours, one and four weeks post injection animals were sacrificed. Blood was collected for enzymes' and hormones' assays. Organs were weighed and testes were taken for histological examination and sperm counts. The results showed that DBCP at a dose of 50 mg kg-1 had a general toxic effect expressed by reduction in body and liver weights and reduced activities of serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT). These changes show a tendency to revert to normal values with time. On the other hand, gonadotoxic effects increase in severity with time. The weight of testes and epididymides were reduced, sperm counts decreased and histological damage advanced, and FSH and LH blood levels increased 4 weeks post injection. It seems that in rats the gonadotoxic effect of DBCP is dissociated from the general toxic effects.
Kaplanski
J
J
Dept. of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel.
Shemi
D
D
Waksman
J
J
Potashnik
G
G
Sod-Moriah
U A
UA
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000969:Antinematodal Agents / Q000633:toxicity; D001219:Aspartate Aminotransferases / Q000097:blood; D005640:Follicle Stimulating Hormone / Q000097:blood; D005837:Genitalia, Male / Q000187:drug effects / Q000473:pathology; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011407:Propane / Q000031:analogs & derivatives* / Q000633:toxicity; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000097:blood
D000969:Antinematodal Agents; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; C007318:1,2-dibromo-3-chloropropane; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D011407:Propane