title: In vitro fertilization of oocytes from polyovular follicles in mouse ovaries exposed neonatally to diethylstilbestrol.
abstract: In 35-day-old female ICR/JCL mice given 5 daily injections of 1 microgram diethylstilbestrol (DES) from the day of birth, a significantly higher incidence of polyovular follicles was found in the ovaries than in those of age-matched control mice. Gap junctions of granulosa cells of mature follicles in neonatally DES-exposed mice were larger than those of the controls. When stimulated by gonadotropins (PMSG and hCG) and caged with males, the number of tubal embryos in DES-exposed mice was less, but the rate of fertilization and development was not different compared to the controls. Division of oocytes collected from the ovaries of 40-day-old DES-exposed and control mice after stimulation of gonadotropins was examined 24 to 72 h after in vitro insemination to ascertain whether fertilization had occurred in oocytes from polyovular follicles. Seventy-seven % of oocytes from uniovular follicles of control mice developed up to 8-cell stage embryos following in vitro insemination; 66% of those from similar follicles of DES-exposed mice developed into the same stage. By contrast, only 47% of oocytes from polyovular follicles of DES-exposed mice showed the division up to 8-cell stage 72 h after insemination, indicating a significantly lower fertilization rate compared to the oocytes from uniovular follicles of control and DES-exposed mice. Without insemination, oocytes taken from the same pools in these experiments never divided during the period of manipulation and incubation.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Biology, Yokohama City University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Iguchi
authors.forename: K
authors.initials: K
authors.lastname: Kamiya
authors.forename: Y
authors.initials: Y
authors.lastname: Uesugi
authors.forename: K
authors.initials: K
authors.lastname: Sayama
authors.forename: N
authors.initials: N
authors.lastname: Takasugi
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004622:Embryo, Mammalian / Q000033:anatomy & histology / Q000187:drug effects*; D005260:Female; D005307:Fertilization in Vitro; D006107:Granulosa Cells / Q000187:drug effects / Q000648:ultrastructure; D007365:Intercellular Junctions / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000187:drug effects / Q000648:ultrastructure; D010053:Ovary / Q000033:anatomy & histology / Q000187:drug effects*; D010063:Ovum / Q000187:drug effects / Q000648:ultrastructure; D011247:Pregnancy; D011248:Pregnancy Complications
chemical_list: D004054:Diethylstilbestrol