Anti-tumoral and anti-inflammatory effects of biological stains.
The biological stains, methylene blue and its metabolite azure B, were evaluated as anti-tumor and anti-inflammatory agents. Azur B, administered in drinking water to tumor-bearing mice, inhibited the growth of transplanted tumors and the growth of primary tumors induced by methylcholanthrene. Inhibition of growth of primary tumors was observed only in female mice. Azure B also reduced the wet weight of carrageenin-induced granulomas in rats. Azure B, given intravenously to BCG-sensitized mice 15 minutes prior to challenge with lipopolysaccharide, decreased TNF production (to 10% of control values) and prevented death from endotoxic shock. Methylene blue decreased TNF production (to 50% of control values) but did not protect the animals from endotoxic shock. Our results suggest that some of the effects previously ascribed to methylene blue are probably mediated via its metabolite, i.e. azure B. Low toxicity and easy administration of the dyes explain their use in clinical settings.
Culo
F
F
Department of Physiology and Immunology, Faculty of Medicine, University of Zagreb, Yugoslavia.
Sabolović
D
D
Somogyi
L
L
Marusić
M
M
Berbiguier
N
N
Galey
L
L
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001399:Azure Stains / Q000627:therapeutic use*; D001500:BCG Vaccine / Q000276:immunology; D002351:Carrageenan; D005260:Female; D005354:Fibrosarcoma / Q000139:chemically induced / Q000188:drug therapy; D006099:Granuloma / Q000139:chemically induced / Q000188:drug therapy; D008070:Lipopolysaccharides; D008297:Male; D008748:Methylcholanthrene; D008751:Methylene Blue / Q000627:therapeutic use*; D051379:Mice; D008808:Mice, Inbred CBA; D009368:Neoplasm Transplantation; D012772:Shock, Septic / Q000188:drug therapy; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000970:Antineoplastic Agents; D001399:Azure Stains; D001500:BCG Vaccine; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D008748:Methylcholanthrene; C025819:Azure B; D002351:Carrageenan; D008751:Methylene Blue