Intrinsic lymphatic partition rate of mepitiostane, epitiostanol, and oleic acid absorbed from rat intestine.
Mepitiostane (MP), epitiostanol (EP), and oleic acid were administered to the jejunal loop of mesenteric vein- and thoracic duct-cannulated rats, and the intrinsic lymphatic partition rate (ILPR) of the absorbed compounds was directly determined. When 14C-EP was administered to the jejunal loop, recovery of unchanged EP in the mesenteric blood and the lymph was 7.9 and 0.03% of the administered dose, respectively. In contrast, following administration of 14C-MP, recovery of unchanged MP in the mesenteric blood and the lymph was 1.2 and 15.0%, respectively. Thus, following passage through the mucosal cell, 99.6% of the unchanged EP was partitioned into the blood and 0.4% into the lymph, while for unchanged MP, 7.6% was partitioned into the blood and 92.4% into the lymph. When 14C-oleic acid was administered to the jejunal loop, most of the penetrating oleic acid was incorporated into triglycerides in epithelial cells and transferred exclusively into the lymph. However, of the unchanged oleic acid, only 37.6% was partitioned into the lymph and 62.4% into the blood. The ILPR was 92.4% for MP, 0.4% for EP, and 37.6% for oleic acid. We conclude that the ILPR values indicate the true lymphotropic property of the compounds.
Ichihashi
T
T
Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
Kinoshita
H
H
Takagishi
Y
Y
Yamada
H
H
D000732:Androstanols / Q000097:blood / Q000493:pharmacokinetics*; D000818:Animals; D001652:Bile Ducts / Q000502:physiology; D005260:Female; D007408:Intestinal Absorption; D008196:Lymph / Q000378:metabolism; D008208:Lymphatic System / Q000378:metabolism*; D008642:Mesenteric Veins / Q000378:metabolism; D019301:Oleic Acid; D009829:Oleic Acids / Q000097:blood / Q000493:pharmacokinetics*; D051381:Rats; D011919:Rats, Inbred Strains
D000732:Androstanols; D009829:Oleic Acids; D019301:Oleic Acid; C006242:mepitiostane; C002976:epitiostanol