title: High-performance liquid chromatography of peptides at reduced temperatures: separation of isomers.
abstract: Cholecystokinin analogues containing N-methyl amino acids were studied by reversed-phase high-performance liquid chromatography at reduced temperatures. A reduction in temperature to -17 degrees C led to lower efficiency, but at the same time separations of cis and trans isomers (and some impurities) were achieved. The velocity constants for cis-trans equilibria were calculated.
authors.affiliation: Department of Chemistry, University of Arizona, Tucson 85721.
authors.forename: M
authors.initials: M
authors.lastname: Lebl
authors.forename: S A
authors.initials: SA
authors.lastname: Fang
authors.forename: V J
authors.initials: VJ
authors.lastname: Hruby
mesh_terms: D000595:Amino Acid Sequence; D002766:Cholecystokinin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D003080:Cold Temperature; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism
chemical_list: D010446:Peptide Fragments; D002766:Cholecystokinin