Isolation and characterization of egg yolk antibodies IgY from hens immunized with different influenza virus strains.
A comparison of two precipitation methods of IgY from normal hen eggs was made. For method I the precipitation agent is represented by PEG 6000, and for method II by organic solvents. The comparative study of some parameters (protein concentration, ovalbumin content, presence of non-specific inhibitors, PAA-gel electrophoresis) shows that method I is more efficient and more convenient than method II. Using this method, we isolated and characterized IgY preparations from hens immunized with circulating influenza virus strains: A/Singapore/6/86 (H1N1), A/Shanghai/11/87 (H3N2) B/Beijing/1/87 and B/Yamagata/16/88 These viral IgY antibody preparations are homogeneous, lacking anti-host cell antibodies and non-specific inhibitors. Their NI titres and HI titres are higher than those found in the sera of immunized birds.
Cuceanu
N
N
Cantacuzino Institute, Bucharest, Romania.
Constantinescu
C
C
Ioniţă
E
E
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000302:isolation & purification*; D002645:Chickens / Q000276:immunology*; D004530:Egg Yolk / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D005260:Female; D006386:Hemagglutination Tests; D007114:Immunization; D005779:Immunodiffusion; D007136:Immunoglobulins / Q000032:analysis / Q000302:isolation & purification*; D009980:Influenza A virus / Q000276:immunology*; D009981:Influenza B virus / Q000276:immunology*; D009439:Neuraminidase / Q000037:antagonists & inhibitors
D000906:Antibodies; C016512:IgY; D007136:Immunoglobulins; D009439:Neuraminidase