Immunogold labelling of human IFN alpha 2 and an IFN alpha 1/ alpha 2 hybrid produced by recombinant Escherichia coli.
In recombinant Escherichia coli strains the subcellular location of human interferon (IFN) alpha 2 and a hybrid IFN alpha 1/alpha 2 was investigated by immunogold labelling techniques. The gold label was scattered throughout the cytoplasm in cells containing the gene for mature IFN alpha 2 under the control of heterologous staphylokinase sak42D transcription and translation initiation signals. In contrast, in cells containing in addition the sak42D signal peptide coding region in front of the IFN gene, the gold label was found mainly near the cell membrane and in the periplasmic space. Inclusion bodies were identified in cells accumulating IFN in the cytoplasm.
Breitling
R
R
Institut für Mikrobiologie und experimentelle Therapie, Jena.
Wagner
B
B
Wagner
M
M
Gase
K
K
Behnke
D
D
D015153:Blotting, Western; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007150:Immunohistochemistry; D016898:Interferon-alpha / Q000032:analysis / Q000235:genetics*; D016253:Microscopy, Immunoelectron / Q000379:methods; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins / Q000032:analysis* / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics
D016898:Interferon-alpha; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins