Effects of plant ribosome-inactivating proteins on ribosomes from Musca domestica.
1. Ribosomes from M. domestica larvae were isolated and their susceptibility to the action of several ribosome-inactivating proteins (RIPs) from plants was tested. 2. Ribosome-inactivating proteins inhibited, to different extents, phenylalanine polymerization by ribosomes. 3. Analysis of RNA from RIP-treated ribosomes showed the appearance of an aniline-cleavable rRNA fragment resulting from the N-glycosidase activity of the RIPs. 4. The release of adenine from saporin 6-treated M. domestica ribosomes was demonstrated by h.p.l.c. analysis.
Ferrari
C
C
Dipartimento di Patologia sperimentale dell' Università di Bologna, Italy.
Barbieri
L
L
Stirpe
F
F
D000225:Adenine / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006793:Houseflies / Q000235:genetics; D000922:Immunotoxins; D007700:Kinetics; D009699:N-Glycosyl Hydrolases; D010455:Peptides / Q000378:metabolism; D010940:Plant Proteins / Q000494:pharmacology*; D012335:RNA, Ribosomal / Q000378:metabolism*; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000378:metabolism*; D000076985:Saporins
D000922:Immunotoxins; D010455:Peptides; D010940:Plant Proteins; D012335:RNA, Ribosomal; D054789:Ribosome Inactivating Proteins, Type 1; C012001:polyphenylalanine; D009699:N-Glycosyl Hydrolases; D000076985:Saporins; D000225:Adenine