Release of tumor cytolytic/cytostatic factor(s) by in vitro cisplatin treated macrophages: enhanced tumoricidal activity by increase in osmotic fragility of target cells.
In vitro cisplatin treatment of murine macrophages results in the release of tumor cytolytic/cytostatic factor(s) (TCF) into the culture medium. These cytolytic factors are cytotoxic/cytostatic only against Dalton's lymphoma and L929 tumor cells but not to normal splenocytes. The cisplatin-activated macrophages retain the capacity to lyse the tumor cells even after removal of the medium containing cisplatin. The mechanism of tumoricidal activity by the TCF was investigated. TCF containing medium was found to enhance the osmotic fragility of the tumor cells.
Singh
S M
SM
Department of Zoology, Banaras Hindu University, Varanasi, India.
Sodhi
A
A
Pai
K
K
Geetha
B
B
D000818:Animals; D002945:Cisplatin / Q000494:pharmacology*; D003602:Cytotoxicity, Immunologic; D003603:Cytotoxins / Q000378:metabolism*; D066298:In Vitro Techniques; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D009996:Osmotic Fragility; D014407:Tumor Cells, Cultured / Q000276:immunology
D003603:Cytotoxins; D002945:Cisplatin