The effect of plasma solutes on total-body-water measurements via NMR.
Nuclear magnetic resonance (NMR) spectroscopy has been reported as an alternative method for quantitating deuterium oxide concentrations in the evaluation of total-body-water in humans. However, the presence of dissolved plasma proteins results in an underestimation of deuterium NMR (2H-NMR) intensity ratios, thereby causing an overestimation (5-6%) of total-body-water (TBW) values determined from nonsublimed patient plasma samples. We demonstrate that plasma samples must be corrected for the volume percentage of water in plasma. Correction of initial 2H-NMR intensity ratios with a factor of 0.93 results in intensity ratios comparable to those determined from plasma samples subjected to vacuum sublimation to remove all plasma solutes.
Richards
E W
EW
Department of Research, Baptist Medical Center, Birmingham, Alabama 35211.
Abu Khaled
M
M
Watkins
C L
CL
Long
C L
CL
D000328:Adult; D001798:Blood Proteins / Q000032:analysis; D001823:Body Composition; D001834:Body Water; D003903:Deuterium; D005188:False Negative Reactions; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010949:Plasma / Q000737:chemistry*
D001798:Blood Proteins; D003903:Deuterium