title: A simple reflectance microphotometer for dot immunobinding assay (dot-ELISA) quantification.
abstract: The use of a simple reflectance microphotometer adapted to quantify enzymatic dot immunobinding assays is described. The instrument is versatile and precise by clinical laboratory standards (i.e., 1.1% coefficient of variation for ten consecutive readings of a grey cardboard). The instrument provides a low-cost alternative to commercially available equipment for quantitation of dot-ELISA assays.
authors.affiliation: Departamento de Parasitologia, Universidade de São Paulo, Brasil.
authors.forename: C S
authors.initials: CS
authors.lastname: Ferreira
authors.forename: M U
authors.initials: MU
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015151:Immunoblotting / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood; D010783:Photometry / Q000295:instrumentation*; D010963:Plasmodium falciparum / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G