[Structure-activity study of the HindIII-I fragment of the L-IVP strain of vaccinia virus genome. II. Cloning the I(sub 6) gene and identification of its protein product].
The I6 gene from the HindIII-I-fragment of the vaccinia virus strain L-IVP DNA was cloned into bacterial vector pUC19. The monospecific antiserum to the expression product of this gene in E. coli was obtained. Using this antiserum the I6 gene was shown to code the viral protein of 34 kDa molecular weight estimated from SDS-PAGE. This protein is not included into the mature virion, but can be detected in the cytoplasm of the vaccinia virus infected cells.
Chikaev
N A
NA
Muravlev
A I
AI
Netesova
N A
NA
Malygin
E G
EG
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D013329:Structure-Activity Relationship; D014616:Vaccinia virus / Q000235:genetics*; D014760:Viral Fusion Proteins / Q000378:metabolism*
D004279:DNA, Viral; D014760:Viral Fusion Proteins
Strukturno-funktsional'noe issledovanie HindIII-I-fragmenta genoma virusa ospovaktsiny shtamma L-IVP. II. Klonirovanie gena I(6) i identifikatsiia ego belkovogo produkta.