Acid phosphatase and arylsulphatase activities in testes after AET or MEA treatment of adult mice.
Temporal changes of acid phosphatase (E.C. 3.1.3.2) and arylsulphatase (E.C. 3.1.6.1) activities in testes of adult Swiss mice after AET (2-amino-ethylisothiouronium Br. HBr) or MEA (cysteamine HCl) treatment, were studied. The animals were injected intraperitoneally with the S-containing substances in a single dose of 400 mg/kg body weight. The enzyme activities in crude organ homogenates were assessed every four hours during a 24-hour period. Administration of the aminothiol agents to mouse organism caused greater changes in the acid phosphatase activity than in the arylsulphatase activity, and the two chemical compounds AET and MEA given, influenced the enzyme activities in testes in a different way. Treatment of mice with AET resulted in a decrease of the acid phosphatase activity related to 1 g of fresh tissue at 16.00 and the whole organ weight at 24.00 and 16.00 as well as in a decrease of the arylsulphatase activity expressed per the whole weight of testes at 08.00. After MEA injection, the acid phosphatase activity related to 1 mg of protein, 1 g of fresh tissue and the whole organ weight was decreased at 20.00(1), and the enzyme activity expresse per 1 mg of protein and 1 g of fresh tissue was increased at 24.00, but the arylsulphatase activity related to both 1 mg of protein at 08.00, 12.00 and to the whole weight of testes at 08.00, was reduced.
Mazur
L
L
Department of Animal Physiology, Jagiellonian University, Cracow, Poland.
Manowska
J
J
Bobik
R
R
D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism*; D003543:Cysteamine / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009929:Organ Size; D013737:Testis / Q000187:drug effects* / Q000201:enzymology; D000338:beta-Aminoethyl Isothiourea / Q000494:pharmacology*
D000338:beta-Aminoethyl Isothiourea; D003543:Cysteamine; D000135:Acid Phosphatase; D001192:Arylsulfatases