Enzyme immunoassay to determine serum myoglobin in patients with acute myocardial infarction.
A method of "sandwich" enzyme immunoassay was developed for determination of human serum myoglobin with the use of myoglobin isolated from human myocardium and gammaglobulin fraction of a specific sheep antiserum labelled with horseradish peroxidase. The linear part of the calibration curve within the range of 0.08-2.2 nmol/l is suitable for accurate quantitative reading of myoglobin concentration. Intra- and interassay variation coefficients are 7% and 11.2%, respectively. A comparison of 100 serum samples assessed by means of commercially available RIA kit and by the given method revealed a correlation coefficient of 0.86.
Kurcová
I
I
Department of Medical Chemistry, Charles University Medical School, Prague, Czechoslovakia.
Tlaskalová
H
H
Srámek
M
M
Bouzek
J
J
Mancal
P
P
Malbohan
I
I
D004797:Enzyme-Linked Immunosorbent Assay; D005500:Follow-Up Studies; D006801:Humans; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis; D009206:Myocardium / Q000378:metabolism; D009211:Myoglobin / Q000097:blood*; D012016:Reference Values
D009211:Myoglobin