title: Absence of mutagenic effects of continuous and pulsed ultrasound in cultured (AL) human-hamster hybrid cells.
abstract: Mutagenic effects of continuous and pulsed ultrasound were looked for using an in vitro assay system, the AL hybrid, that is up to 100 times more sensitive for mutagens such as x-rays and neutrons than the assays used previously to evaluate ultrasound. Cells in suspension in rotated plastic test tubes were insonated with continuous wave ultrasound at 1 MHz, ISPTP = 0.62-40 W/cm2 for 0-40 min. Cells attached in the central region of culture flasks received pulsed exposures at fc = 2.5 MHz, PRF = 1 kHz, 2 and 8 cycles per pulse, with p- = 1.2 MPa (ISPTA = 31-180 mW/cm2) for 0-30 min. Although these exposures were cytotoxic (the plating efficiency was decreased to approximately 65% by the highest doses), induction of mutation, if any occurred, was less than would be expected in this test system from 10-30 cGy of x-ray.
authors.affiliation: Dept. of Radiology, University of Colorado School of Medicine, Denver 80262.
authors.forename: E R
authors.initials: ER
authors.lastname: Ritenour
authors.forename: M
authors.initials: M
authors.lastname: Braaton
authors.forename: G H
authors.initials: GH
authors.lastname: Harrison
authors.forename: A
authors.initials: A
authors.lastname: Ueno
authors.forename: M
authors.initials: M
authors.lastname: Gadd
authors.forename: M
authors.initials: M
authors.lastname: Manco-Johnson
authors.forename: R
authors.initials: R
authors.lastname: Parker
authors.forename: S
authors.initials: S
authors.lastname: Shih
authors.forename: C A
authors.initials: CA
authors.lastname: Waldren
mesh_terms: D000818:Animals; D002452:Cell Count; D002470:Cell Survival; D002478:Cells, Cultured; D006168:Guinea Pigs; D006801:Humans; D006822:Hybrid Cells / Q000000981:diagnostic imaging* / Q000528:radiation effects; D016296:Mutagenesis; D009152:Mutagenicity Tests; D014463:Ultrasonography; D014965:X-Rays