Materials for enhancing cell adhesion by immobilization of cell-adhesive peptide.
Materials to enhance cell adhesion were synthesized by surface integration of peptide, Arg-Gly-Asp-Ser(RGDS), which is an active-site sequence of cell-adhesive proteins. Polystyrene film was glow-discharged and graft-copolymerized with acrylic acid. Then the peptide was immobilized to the poly(acrylic acid) grafts by using water-soluble carbodiimide. The cell-adhesive activity of the RGDS-immobilized film increased with increasing amount of immobilized peptide, and approached the activity of fibronectin(FN)-immobilized film. The RGDS-immobilized film was more stable against heat treatment and pH variation than the FN-immobilized film. In addition, the RGDS-immobilized film enhanced cell growth more strongly than the FN-immobilized film.
Ito
Y
Y
Department of Polymer Chemistry, Kyoto University, Japan.
Kajihara
M
M
Imanishi
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D005353:Fibronectins; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides; D011137:Polystyrenes; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D005353:Fibronectins; D010455:Peptides; D011137:Polystyrenes