title: [The effect of Mg-ADP on the structural state of actin in the F-actin-myosin subfragment-1 complex].
abstract: Using polarized microfluorometry techniques, a study was made on the orientation and mobility of fluorescent probes 1,5-IAEDANS and rhomadin-phalloidin, located in various parts of actin, muscle fibers free of myosin, tropomyosin and troponin (ghost fibres) being used. It was found that the binding of a myosin subfragment 1 (S1) to actin induced changes in polarized fluorescence of the fibers. The analysis of these data showed that the formation of actin-S1 and actin-S1-ADP complexes in a muscle fiber resulted in a decrease in the angle between the thin filaments and the emission dipole of phalloidin-rhodamine, as well as in an increase of the mobility of this dye. In the experiments with the 1,5-IAEDANS label the angle of emission dipole increased, while the mobility of the label decreased. These changes were smaller in the presence of Mg-ADP than in its absence. It is assumed that the changes in actin monomer structure occur when a myosin head interacts with actin. These changes are expressed as those in orientation and mobility of large and small domains of actin in thin filaments. The domain orientation in actomyosin complex changes, influenced by Mg-ADP. The data obtained allow to propose the involvement of interdomain motions of some parts of actin monomer in the mechanisms of muscle contraction.
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Borovikov
authors.forename: V P
authors.initials: VP
authors.lastname: Kirillina
mesh_terms: D000199:Actins / Q000187:drug effects* / Q000648:ultrastructure; D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000818:Animals; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D015879:Myosin Subfragments / Q000187:drug effects* / Q000648:ultrastructure; D009282:Naphthalenesulfonates; D010590:Phalloidine; D011817:Rabbits; D012235:Rhodamines
chemical_list: D000199:Actins; D005456:Fluorescent Dyes; D015879:Myosin Subfragments; D009282:Naphthalenesulfonates; D012235:Rhodamines; D010590:Phalloidine; D000244:Adenosine Diphosphate; C006421:1,5-I-AEDANS
vernacular_title: Vliianie Mg-ADF na strukturnoe sostoianie aktina v komplekse F-aktin--subfragment-1 miozina.