Purification of a new restriction endonuclease from Streptococcus mutans and identification of its recognition sequence.
SmuE I, a type II restriction endonuclease, has been isolated from Streptococcus mutans serotype E, which is an isoschizomes of Ava II recognizes the palidromic pentanucleotide sequence 5' GG/W/CC 3'. Similarly to Ava II, SmuE I cleaves the sequence, G--G/W/CC, generating 5' protruding fragment termini.
Molnár
A
A
Institute of Microbiology, Semmelweis University Medical School, Budapest, Hungary.
Geck
P
P
Orosz
A
A
Kulcsár
P
P
Nász
I
I
D001483:Base Sequence; D002850:Chromatography, Gel; D003470:Culture Media; D004279:DNA, Viral / Q000737:chemistry* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D009154:Mutation; D010957:Plasmids; D015183:Restriction Mapping; D013295:Streptococcus mutans / Q000201:enzymology*
D003470:Culture Media; D004279:DNA, Viral; D015252:Deoxyribonucleases, Type II Site-Specific; C038308:GGWCC-specific type II deoxyribonucleases