Seroepidemiology of anti-Entamoeba histolytica antibody by enzyme-linked immunosorbent assay in the greater Tokyo area.
An enzyme-linked immunosorbent assay (ELISA) for detecting antibody against Entamoeba histolytica was used in a survey to determine the prevalence of E. histolytica infection in the greater Tokyo area. Serum samples were collected from 1,869 randomly chosen, symptom-free volunteers (1,645 males and 224 females). Seventeen individuals (0.91%), 14 males and three females, had ELISA values that were interpreted as positive. The calculated histogram of the anti-E. histolytica IgG values, after logarithmic transformation of the original skewed values, revealed a normal (Gaussian) distribution. It was also found that the results of the ELISA were closely correlated with those obtained by an indirect immunofluorescent assay (IFA).
Tanaka
T
T
Department of Parasitology, Tokai University School of Medicine, Kanagawa, Japan.
Kaneda
Y
Y
D000328:Adult; D000368:Aged; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D004748:Entamoeba histolytica / Q000276:immunology*; D004749:Entamoebiasis / Q000453:epidemiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D016036:Seroepidemiologic Studies; D014041:Tokyo / Q000453:epidemiology
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G