Induction of heat shock proteins in lymphocytes increases with mitogen stimulation.
The effect of the growth state of a cell on the ability of hyperthermia to induce the synthesis of heat shock proteins (HSPs) was studied in resting and concanavalin A (ConA)-stimulated lymphocytes. Hyperthermia induced the synthesis of hsp 110, hsp 90, hsc 70, and hsp 70 in both resting and ConA-stimulated lymphocytes, and ConA-treatment induced the synthesis of the hsp 90 and hsc 70 at normal temperature. The induction of the synthesis of hsp 110 and hsp 70 by hyperthermia was 3- to 6-fold higher for lymphocytes cultured with ConA for 12 and 24 h than in non-stimulated lymphocytes. Thus, lymphocytes induced to undergo proliferation showed a greater response to hyperthermia than resting lymphocytes.
Ghassemi
M
M
Geriatric, Research, Education and Clinical Center, Audie L. Murphy Memorial Veterans Hospital, San Antonio, TX 78284.
Heydari
A R
AR
Richardson
A
A
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology*; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D013268:Stimulation, Chemical; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism
D006360:Heat-Shock Proteins; D003208:Concanavalin A