Enhanced chemiluminescence for the detection of membrane-bound nucleic acid sequences: advantages of the Amersham system.
A range of nonradioactive nucleic acid labeling and detection systems have been developed that enable the user to label probes directly with enzyme molecules or indirectly with hapten-derivatized nucleotides. Horseradish peroxidase is used for the direct labeling procedures due to the ease of chemical modification and the relative thermal and chemical stability of this enzyme. Horseradish peroxidase has also been conjugated to a high-specificity antifluorescein antibody for detection of hapten (fluorescein)-labeled hybrids. Enhanced chemiluminescence is a light-emitting process optimized for the detection of low levels of horseradish peroxidase on membrane supports. Results are obtained as hard copy images on x-ray film.
Stone
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T
Amersham International, Amersham Laboratories, Buckinghamshire, UK.
Durrant
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I
D001482:Base Composition; D001483:Base Sequence; D002462:Cell Membrane / Q000737:chemistry; D019793:Fluorescein; D005452:Fluoresceins; D006735:Horseradish Peroxidase; D006801:Humans; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000032:analysis*; D015345:Oligonucleotide Probes
D005452:Fluoresceins; D009696:Nucleic Acids; D015345:Oligonucleotide Probes; D006735:Horseradish Peroxidase; D019793:Fluorescein