[Effect of ultraviolet irradiation on properties and physicochemical properties of lactate dehydrogenase].
Catalytic and some physicochemical properties of lactate dehydrogenase have been studied as affected by UV-irradiation. The degree of lactate dehydrogenase inactivation has been determined both at UV-irradiation of a free enzyme and of the enzyme in complex with NADH. It is shown that at pH 7.4 NADH makes a photosensitizing effect on the enzyme. Based on the analysis of changes in the absorption spectrum and molecular weight of lactate dehydrogenase caused by UV-irradiation it is supposed that this irradiation induces the process of unrolling-rolling up of the protein globule.
Artiukhov
V G
VG
Kakvasina
M A
MA
D000818:Animals; D002384:Catalysis; D007770:L-Lactate Dehydrogenase / Q000737:chemistry / Q000528:radiation effects*; D008970:Molecular Weight; D009243:NAD / Q000737:chemistry; D011838:Radiation-Sensitizing Agents / Q000737:chemistry; D013552:Swine; D014466:Ultraviolet Rays
D011838:Radiation-Sensitizing Agents; D009243:NAD; D007770:L-Lactate Dehydrogenase
Vliianie ul'trafioletovogo oblucheniia na kataliticheskie i fiziko-khimicheskie svoĭstva laktatdegidrogenazy.