title: Highly specific and highly sensitive enzyme immunoassays for antibodies to human interleukin 3 (IL-3) and human erythropoietin (EPO) in serum.
abstract: Two versatile, rapid, easy-to-perform and highly sensitive enzyme immunoassays (ELISAs) were developed for the detection and quantification of antibodies to human IL-3 and human EPO in serum samples from both man and laboratory animals. These one-step assays are based on the "inverse sandwich" principle, where antibodies present in the sample linked both the solid phase antigen bound to a microtiter plate and the free antigen, which had been covalently coupled to horseradish peroxidase. The limits of detection are lower than those of the neutralization bioassays; antibodies to IL-3 and to EPO were detected at concentrations as low as 2 ng/ml and 5 ng/ml, respectively. No cross-reactivity with related proteins and no interfering matrix effects were observed when used for the estimation of antibodies against rhu IL-3 and rhu EPO in serum samples of various species. Thus both assays were used without modification for the screening of antibodies in serum samples from both man and animal during treatment with these hematopoietic hormones.
authors.affiliation: Research Laboratories of Behringwerke AG, Marburg/Lahn, Germany.
authors.forename: J U
authors.initials: JU
authors.lastname: Pauly
authors.forename: H P
authors.initials: HP
authors.lastname: Harthus
authors.forename: F R
authors.initials: FR
authors.lastname: Seiler
mesh_terms: D000818:Animals; D000830:Animals, Laboratory; D000906:Antibodies / Q000032:analysis*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004921:Erythropoietin / Q000097:blood / Q000276:immunology*; D006801:Humans; D007202:Indicators and Reagents; D007377:Interleukin-3 / Q000097:blood / Q000276:immunology*; D008832:Microchemistry; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000906:Antibodies; D007202:Indicators and Reagents; D007377:Interleukin-3; D011994:Recombinant Proteins; D004921:Erythropoietin