title: Interlaboratory evaluation of three culture media for postimplantation rodent embryos.
abstract: The first aim of the study was to compare the ability of rat serum, human serum, and a mixture of human and rat serum (4:1) to support in vitro development of rodent postimplantation embryos. The comparison was made in three laboratories using rat embryos and in one laboratory using mouse embryos. Batches of sera, initial developmental stage, duration of culture, and endpoints were identical in the laboratories. The second aim of the study was to evaluate if other variables that could not be standardized would significantly influence the results of the laboratories. No reproducible difference was observed among the culture media or among the laboratories except that growth and differentiation were slower in the laboratory using mouse embryos. Further experiments are needed to exclude small differences in performance of the media.
authors.affiliation: Laboratory of Developmental Genetics, U.C.L., Louvain-la-Neuve, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Van Maele-Fabry
authors.forename: J J
authors.initials: JJ
authors.lastname: Picard
authors.forename: P
authors.initials: P
authors.lastname: Attenon
authors.forename: P
authors.initials: P
authors.lastname: Berthet
authors.forename: F
authors.initials: F
authors.lastname: Delhaise
authors.forename: M J
authors.initials: MJ
authors.lastname: Govers
authors.forename: P W
authors.initials: PW
authors.lastname: Peters
authors.forename: A H
authors.initials: AH
authors.lastname: Piersma
authors.forename: B P
authors.initials: BP
authors.lastname: Schmid
authors.forename: J
authors.initials: J
authors.lastname: Stadler
mesh_terms: D000818:Animals; D000013:Congenital Abnormalities / Q000473:pathology; D003470:Culture Media; D046508:Culture Techniques; D004622:Embryo, Mammalian / Q000502:physiology*; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D051379:Mice; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D003470:Culture Media