Ultrastructural localization of gold-labeled anti-IgM-antibodies and type A retrovirus particles in endoplasmic cisternae and vesicles of IgM-producing human B cell hybridomas.
Simultaneous appearance of IgM and type A retro-virus particles in endoplasmic cisternae and vesicles of human hybridomas has been demonstrated for the first time by immunogold-labeling at ultrastructural level. A suspected link between type A particle and IgM gene-expression in hybridoma cells could not be substantiated in this study.
Ladhoff
A M
AM
Abteilung für Ultrastrukturelle Pathologie und Elektronenmikroskopie, Medizinische Fakultät der Humboldt-Universität zu Berlin, Deutschland.
Becker
M
M
Jahn
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure; D006801:Humans; D006825:Hybridomas / Q000276:immunology / Q000382:microbiology / Q000648:ultrastructure*; D007075:Immunoglobulin M / Q000032:analysis / Q000096:biosynthesis*; D007150:Immunohistochemistry; D051379:Mice; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D010950:Plasma Cells / Q000648:ultrastructure; D012190:Retroviridae / Q000648:ultrastructure*; D014771:Virion / Q000648:ultrastructure*
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M