[Autologous bone marrow transplantation after purging by low molecular weight natural tumor suppressor in long-term marrow culture in vitro: report on 2 cases].
In the light of high sensitivity of fresh bone marrow blast progenitor of human myeloid leukemia (L-CFU) towards the action of low molecular tumor suppressor isolated from human fetal liver, massive bone marrow was aspirated from the patients suffering from acute myeloid leukemia at the stage of first complete remission and cocultured in a long-term bone marrow culture system for 9 days. The purged marrow cells were then re-infused into the patients after preconditioning with large doses of cytotoxin and acute ionizing irradiation. Hematopoiesis was reconstituted due to the autologous transplantation in vivo. After the autologous bone marrow transplantation without chemical treatment, two cases are still survival with the time of more than 17 and 15 months respectively.
Yang
K
K
Department of Hematology, General Hospital of Beijing Unit, People's Liberation Army.
Ten
A P
AP
Wu
A Y
AY
D000328:Adult; D016465:Bone Marrow Purging / Q000379:methods*; D016026:Bone Marrow Transplantation; D005500:Follow-Up Studies; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology / Q000601:surgery*; D015479:Leukemia, Myelomonocytic, Acute / Q000473:pathology / Q000601:surgery*; D008297:Male; D014182:Transplantation, Autologous; D014407:Tumor Cells, Cultured