Accumulation of sulfoglycolipids in hyperosmosis-resistant clones derived from the renal epithelial cell line MDCK (Madin-Darby canine kidney cell).
1. Two clones (osmR-A and osmR-B) resistant to hyperosmotic media of 700 and 800 mosmol/l, respectively, were selected from Madin-Darby canine kidney (MDCK) cells. 2. When cultured in isosmotic medium (300 mosmol/l), the concentration of galactosyl sulfatide and lactosyl sulfatide in these hyperosmosis-resistant clones was 3.4-5.9 times higher than in the wild-type MDCK. The rate of incorporation of [35S]sulfate into sulfolipids of osmR-A and osmR-B was 1.9-6.7 times higher than MDCK. 3. The stimulation of incorporation into sulfolipids by hyperosmotic culture was completely inhibited by cycloheximide. The pulse-chase studies indicated decreased turnover rate of sulfolipids in osmR-A.
Niimura
Y
Y
Department of Biochemistry, Teikyo University School of Medicine, Tokyo, Japan.
Ishizuka
I
I
D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D003513:Cycloheximide / Q000494:pharmacology; D004285:Dogs; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D006017:Glycolipids / Q000378:metabolism*; D006982:Hypertonic Solutions; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D007700:Kinetics; D013433:Sulfoglycosphingolipids / Q000378:metabolism*
D006017:Glycolipids; D006982:Hypertonic Solutions; D013433:Sulfoglycosphingolipids; C011117:sulfoglycolipids; D003513:Cycloheximide