A novel enzyme immunoassay for quantitation of rat prothrombin in microsomal subfractions.
An enzyme linked immunoassay (ELISA) for quantitation of rat prothrombin, based on a biotin-streptavidin alkaline phosphatase system is described. The assay utilizes rabbit antiserum raised against purified rat prothrombin. The assay was twenty fold more sensitive than a rat prothrombin assay based on amidolytic activity following activation by Echis carinatus venom. Results obtained with the two assays show good correlation. The ELISA is a valuable tool for quantitation of minute amounts of prothrombin in subcellular fractions and large series of plasma samples.
Myrset
A H
AH
Department of Biochemistry, University of Oslo, Norway.
Helgeland
L
L
D000469:Alkaline Phosphatase; D000818:Animals; D001426:Bacterial Proteins; D001710:Biotin; D010450:Endopeptidases; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000276:immunology; D008862:Microsomes, Liver / Q000737:chemistry*; D011516:Prothrombin / Q000032:analysis*; D011817:Rabbits; D051381:Rats / Q000097:blood*; D011919:Rats, Inbred Strains / Q000097:blood; D012680:Sensitivity and Specificity; D019809:Streptavidin
D001426:Bacterial Proteins; D007074:Immunoglobulin G; D001710:Biotin; D011516:Prothrombin; D019809:Streptavidin; D000469:Alkaline Phosphatase; D010450:Endopeptidases; C020097:ecarin