[New findings on the biotyping of Staphylococcus aureus isolated during milk processing].
A detailed analysis of biotypes of Staphylococcus aureus, as related to their origin and enterotoxigenicity, was performed, using 432 strains isolated from bulk milk, milking machines, quarter milk samples collected from mastitic cows, and cowherds and milkers. All strains coagulated rabbit blood plasma and produced thermonuclease (Tab. I). Human strains differed from bovine ones mostly in the production of alpha-haemolysin (94%) and fibrinolysin (66%). Biotypes C1 (35%) and C2 (38%) dominated clearly among the strains isolated from quarter milk samples. The findings of 13% of biotype A and 8% of biotype D suggest that other sources of udder infections than mastitic cows were involved. Almost 19% of human strains and two strains isolated from quarter milk samples were identified as the recently defined type G. The production of enterotoxins (Tab. III) of was associated mostly with strains of human origin (69%) and with biotypes G (35%) and A (31%). Three enterotoxigenic strains belonged to the biotype B and one strain was not classifiable.
Růzicková
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Výzkumný ústav veterinárního lékarství, Brno.
D000818:Animals; D015373:Bacterial Typing Techniques; D002417:Cattle; D003612:Dairying; D004768:Enterotoxins / Q000096:biosynthesis; D006801:Humans; D008414:Mastitis, Bovine / Q000382:microbiology; D008892:Milk / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification* / Q000378:metabolism
D004768:Enterotoxins
Nové poznatky v biotypizaci Staphylococcus aureus z prvovýroby mléka.