[The trypanocidal action of fluorouracil on Crithidia oncopelti in the presence of lipid metabolic modifiers].
Effect of fluorouracil in combination with ascorbate and L-valin, modifiers of lipid metabolism was studied in cultured protozoa Crithidia oncopelti. The inhibitory effect of preparation in a concentration of 100 mcg/ml gradually decreased in the course of cultivation. Its readdition to a 96-hour culture did not increase its effect on protozoa cells. Combined addition of fluorouracil and modifiers (100 mcg/ml each) resulted in insignificant decrease of the cell accumulation in the culture as compared with the effect of fluorouracil alone. When fluorouracil and modifiers were readded to the 96-hour culture, the trypanostatic effect of preparation was 2.5 times enhanced. This enhancement was confirmed by destructive alterations in cell morphology and by the culture lysis by 192 h of protozoa cultivation.
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SUkhanova
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D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D003421:Crithidia / Q000187:drug effects* / Q000378:metabolism; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D004357:Drug Synergism; D005472:Fluorouracil / Q000494:pharmacology*; D008055:Lipids / Q000037:antagonists & inhibitors*; D013997:Time Factors; D014344:Trypanocidal Agents / Q000494:pharmacology*; D014633:Valine / Q000494:pharmacology
D008055:Lipids; D014344:Trypanocidal Agents; D014633:Valine; D001205:Ascorbic Acid; D005472:Fluorouracil
Tripanotsidnoe deĭstvie ftoruratsila na Crithidia oncopelti v prisutstvii modifikatorov lipidnogo metabolizma.