Whole-cell recordings from sympathetic preganglionic neurons in rat spinal cord slices.
Whole-cell patch-clamp recordings (WCR) were made from sympathetic preganglionic neurons (SPN) in neonate rat spinal cord slices. SPN were identified histologically by filling them with the fluorescent dye Lucifer Yellow contained within the patch pipette solution. Current clamp recordings were obtained from SPN with a potassium based pipette solution. The cells exhibited many of the characteristic properties of SPN seen previously with intracellular recordings in both the rat and the cat. However, we found an order of magnitude increase in both cell input resistance (950 M omega) and time constant (118 ms) over those seen with conventional recordings. We believe these values approximate better the situation in intact cells, and will have a vital bearing upon how SPN integrate inputs. We conclude that WCR in spinal cord slices provides a powerful tool for investigating the cellular properties of SPN.
Pickering
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Department of Physiology, Medical School, University of Birmingham, U.K.
Spanswick
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Logan
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D000200:Action Potentials; D000818:Animals; D000831:Animals, Newborn; D001339:Autonomic Fibers, Preganglionic / Q000502:physiology*; D003712:Dendrites / Q000648:ultrastructure; D004558:Electric Stimulation; D007546:Isoquinolines; D009474:Neurons / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology*
D007546:Isoquinolines; C017475:lucifer yellow