[Determination of gomisin A (TJN-101) and its metabolite in rat serum by gas chromatography-mass spectrometry].
Gomisin A (TJN-101) is one of the lignan components isolated from Schisandra Fruits. A high sensitive and precise method for the determination of TJN-101 and its major metabolite (Met. B) in the rat serum was developed by selected ion monitoring (SIM) with gas chromatography-mass spectrometry (GC/MS) using a fused silica capillary column (SPB-1, Supelco). A 100 microliter serum sample was used for the solid phase extraction. The calibration curves of TJN-101 and Met.B both showed a good linearity between 2.0 and 2000.0 ng/ml. The analytical precision (intra-assay, C.V. less than 4.7%), recoveries (98.4 +/- 10.1%), and detection limit (2 ng/ml) of TJN-101 indicated that this system was suited for the determination of TJN-101 in biological fluid. In case of Met.B, the same results as TJN-101, were obtained. After oral administration of TJN-101 at a dose of 10 mg/kg to male rats, the average values of the maximal serum concentration of TJN-101 and Met.B were 1446.1 +/- 131.8 and 317.4 +/- 18.5 ng/ml, respectively. The serum concentrations of these substances could be monitored sufficiently for 8 h after dosing.
Matsuzaki
Y
Y
Research Institute for Pharmacology, Tsumura & Co., Ibaraki, Japan.
Ishibashi
E
E
Koguchi
S
S
Wakui
Y
Y
Takeda
S
S
Aburada
M
M
Oyama
T
T
D000818:Animals; D034242:Cyclooctanes; D004149:Dioxoles; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D017705:Lignans; D008297:Male; D008991:Monitoring, Physiologic; D011083:Polycyclic Compounds / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D011919:Rats, Inbred Strains
D034242:Cyclooctanes; D004149:Dioxoles; D017705:Lignans; D011083:Polycyclic Compounds; C033585:schizandrol B