[Korazol-induced kindling in animals with different sensitivities to the epileptogen].
To select homogeneous groups of sensitive and low-sensitive animals (male Wistar rats) for subsequent kindling experiments the animals's reaction to the threshold dose of pentylenetetrazole (PTZ) (40 mg/kg, i.p.) was defined. Rats showing convulsive response of 1 to 3 scores (seizures were estimated according to a 6-score scale) were assumed to be sensitive animals. Rats when injected with this dose showing no seizures were defined as low-sensitive animals. One week after the test kindling was started by daily administration of a subconvulsive dose of PTZ (30 mg/kg, i.p.). Low-sensitive animals displayed a 3 day delay in the development of kindling seizures and a decrease in the severity of seizures as well as an extended latency period before the first manifestations of seizures after each injection of PTZ. Thus testing by means of the threshold dose of PTZ is a comparatively simple method of preliminary estimation of the animals's sensitivity to this convulsant in order to select groups of relatively sensitive and low-sensitive animals in PTZ kindling experiments. For a more precise selection of animals it is suggested to be useful to repeat the initial test after an interval of 5-7 days. The proposed method seems to be applied in principle to other convulsants as well.
Kryzhanovskiĭ
G N
GN
Karpova
M N
MN
Pankov
O Iu
OIu
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004361:Drug Tolerance; D007696:Kindling, Neurologic / Q000187:drug effects*; D008297:Male; D010433:Pentylenetetrazole / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D011930:Reaction Time / Q000187:drug effects; D012640:Seizures / Q000139:chemically induced*; D013997:Time Factors
D010433:Pentylenetetrazole
Korazolovyĭ kindling u zhivotnykh s razlichnoĭ chuvstvitel'nost'iu k épiteptogenu.