Sequence of a new tRNA(Leu)(U*AA) from brewer's yeast.
The nucleotide sequence of a new tRNA(Leu)(anticodon U*AA) from Saccharomyces cerevisiae which could recognize exclusively the UUA codon has been determined. Its primary structure is: pGGAGGGUUGm2GCac4CGAGDGmGDCDAAGGCm2(2)GGCAGACmUU*AAm1GA++ + psi CUGUUGGACGGUUGUCCGm5CGCGAGT psi CGm1A(orA)ACCUCGCAUCCUUCACCA. This tRNA has a large extraloop and contains 15 modified nucleotides. So far it is the third isoacceptor tRNA for leucine in yeast. It has 61% homology with tRNA(Leu)(anticodon m5CAA) and 63% homology with tRNA(Leu)(anticodon UAG), the two other known yeast tRNAs(Leu).
el Adlouni
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Institut de Biologie Moléculaire et Cellulaire, Université Louis Pasteur, Strasbourg, France.
Desgrès
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Dirheimer
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Keith
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D001483:Base Sequence; D003062:Codon; D015180:Electrophoresis, Gel, Two-Dimensional; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012356:RNA, Transfer, Leu / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D003062:Codon; D012356:RNA, Transfer, Leu