Measuring of the in-vitro assembly of microtubules by dynamic light scattering.
The kinetics of in-vitro assembly of microtubule protein (MTP) to microtubules (MTs) was followed under various conditions (temperature, GTP, ultrasonic treatment) by dynamic light scattering (DLS) and turbidimetric measurements. The results of both methods roughly coincide, but DLS additionally allows to differentiate between MTs and aggregates and to follow their growth. The complexity of these investigations, however, causes serious restrictions in the interpretation of the DLS data.
Walter
A
A
Institute of Microbiology and Experimental Therapy, Jena, Germany.
Gast
K
K
Vater
W
W
Zirwer
D
D
D000818:Animals; D001923:Brain Chemistry; D006160:Guanosine Triphosphate / Q000096:biosynthesis; D008027:Light; D008868:Microtubule Proteins / Q000096:biosynthesis; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D009391:Nephelometry and Turbidimetry; D012542:Scattering, Radiation; D013552:Swine; D013696:Temperature; D014465:Ultrasonics
D008868:Microtubule Proteins; D006160:Guanosine Triphosphate