Characterization of acyllipid: sterol glucoside acyltransferase from oat (Avena sativa L.) seedlings.
Membranous fractions from leaves of oat seedlings readily convert cholesterol beta-D-glucoside into its 6'-O-acyl derivative using endogenous acyllipids as acyl sources. Experiments with delipidated enzyme preparations showed that among acyllipids present in oat leaves digalactosyldiacylglycerols are evidently the best acyl donors in this reaction. Beside of sterol glucosides, the enzyme can acylate beta-D-glucosides of several other steroids, although at very different rates.
Misiak
M
M
Department of Biochemistry, Warsaw University, Poland.
Kalinowska
M
M
Wojciechowski
Z A
ZA
D000215:Acylation; D000217:Acyltransferases / Q000737:chemistry*; D002784:Cholesterol / Q000031:analogs & derivatives / Q000737:chemistry; D002523:Edible Grain / Q000201:enzymology*; D005960:Glucosides / Q000737:chemistry*; D012639:Seeds / Q000201:enzymology; D012855:Sitosterols / Q000031:analogs & derivatives; D013379:Substrate Specificity
D005960:Glucosides; D012855:Sitosterols; C027578:cholesteryl glucoside; D002784:Cholesterol; D000217:Acyltransferases