High-performance liquid chromatography of peptides at reduced temperatures: separation of isomers.
Cholecystokinin analogues containing N-methyl amino acids were studied by reversed-phase high-performance liquid chromatography at reduced temperatures. A reduction in temperature to -17 degrees C led to lower efficiency, but at the same time separations of cis and trans isomers (and some impurities) were achieved. The velocity constants for cis-trans equilibria were calculated.
Lebl
M
M
Department of Chemistry, University of Arizona, Tucson 85721.
Fang
S A
SA
Hruby
V J
VJ
D000595:Amino Acid Sequence; D002766:Cholecystokinin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D003080:Cold Temperature; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism
D010446:Peptide Fragments; D002766:Cholecystokinin