title: Oxidative and assimilative enzyme activities in continuous cultures of the obligate methylotroph Methylobacillus flagellatum.
abstract: In methanol-limited continuous cultures of the obligate methylotrophic bacterium Methylobacillus flagellatum grown at rates from 0.05 to 0.63 h-1, and also in an oxyturbidostat culture of M. flagellatum growing at the rate of 0.73 h-1, levels of methanol dehydrogenase, enzymes of formaldehyde oxidation (both linear and cyclic) and assimilation (RuMP cycle), a number of intermediary metabolism and TCA cycle enzymes and also 'dye-linked' formaldehyde dehydrogenase were determined. It was shown that the activities of dissimilatory enzymes, with the exception of 'dye-linked' formaldehyde dehydrogenase, decreased with increasing growth rate. Activities of assimilative enzymes and activities of the TCA cycle enzymes detected as well as the 'dye-linked' formaldehyde dehydrogenase activity, increased with increasing growth rate. A periplasmic location was shown for the latter enzyme and a role in formaldehyde detoxification was proposed.
authors.affiliation: Institute of Genetics and Selection of Industrial Microorganisms, Moscow, USSR.
authors.forename: L V
authors.initials: LV
authors.lastname: Chistoserdova
authors.forename: A Y
authors.initials: AY
authors.lastname: Chistoserdov
authors.forename: N L
authors.initials: NL
authors.lastname: Schklyar
authors.forename: M V
authors.initials: MV
authors.lastname: Baev
authors.forename: Y D
authors.initials: YD
authors.lastname: Tsygankov
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D002455:Cell Division; D003470:Culture Media; D003593:Cytoplasm / Q000737:chemistry / Q000201:enzymology; D007124:Immunoenzyme Techniques; D000432:Methanol / Q000378:metabolism*; D008772:Methylococcaceae / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism
chemical_list: D003470:Culture Media; C020145:formaldehyde dehydrogenase (glutathione); D000445:Aldehyde Oxidoreductases; D000432:Methanol