Location of ribosomal genes in CHO cells; in situ hybridization with a non-isotopic DNA probe on G-banded chromosomes.
A novel in situ hybridization technique using sulfonated probes is described. This non-radioactive approach, which employs chemically modified DNA and immunocytochemical procedures, is compatible with pre-G-banding and allows a rapid localization of the hybridized sequences on chromosomal spreads with a high spatial resolution. Using this technique we have localised the Chinese hamster ribosomal genes in the telomeric region of ten chromosomes, and among them in the subtelomeric q region of the Z5 chromosome. These results are discussed, the genetic markers confirming and locating the origin of Z group chromosomes by rearrangements of Chinese hamster chromosomes.
Azum-Gelade
M C
MC
Centre de Recherches de Biochimie et de Génétique Cellulaires du CNRS, Toulouse, France.
Noaillac-Depeyre
J
J
Gas
N
N
D000818:Animals; D016466:CHO Cells; D002871:Chromosome Banding; D002875:Chromosomes / Q000648:ultrastructure*; D006224:Cricetinae; D015342:DNA Probes; D004275:DNA, Ribosomal / Q000032:analysis*; D005796:Genes; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping
D015342:DNA Probes; D004275:DNA, Ribosomal