Transformation of a methylotrophic bacterium, Methylobacterium extorquens, with a broad-host-range plasmid by electroporation.
Electroporation was used to transform the methylotrophic bacterium Methylobacterium extorquens with broad-host-range plasmid pLA2917, which contains a gene specifying resistance to kanamycin. Plasmid DNA was introduced into M. extorquens in the presence of an electric pulse, and kanamycin-resistant transformants were obtained. These transformants harbored plasmid DNA that was identical to plasmid pLA2917. We examined several factors independently and found up to 8 x 10(3) transformants per microgram of DNA using 10 pulses with a duration of 300 microseconds at a field strength of 10 kV/cm.
Ueda
S
S
Department of Food Science and Technology, School of Agriculture, Nagoya University, Japan.
Matsumoto
S
S
Shimizu
S
S
Yamane
T
T
D002470:Cell Survival; D004269:DNA, Bacterial / Q000235:genetics; D004560:Electricity; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial; D006088:Gram-Negative Aerobic Bacteria / Q000235:genetics*; D010957:Plasmids; D014170:Transformation, Genetic
D004269:DNA, Bacterial