Correlation between DNA polymorphism and enzyme polymorphism argues in favour of the delineation of two species within Providencia alcalifaciens.
Ribosomal DNA (rDNA) polymorphism was compared to enzyme polymorphism and DNA/DNA hybridization data for the intraspecies differentiation of Providencia alcalifaciens. DNA from 27 strains previously classified into two zymotypes A1 and A2 and discriminated by two levels of DNA/DNA hybridization (delta Tm values of 0 to 1 degree C and 6 to 10 degrees C, respectively) were analysed by Southern blotting for rDNA polymorphism. The ribotypes fell into two ribogroups A1 and A2, which correlated with the corresponding zymotypes. This correlation argues for the proposed creation of two species within P. alcalifaciens.
Picard
B
B
Laboratoire de Microbiologie, Faculté de Médecine Xavier Bichat, Université Paris VII.
Picard-Pasquier
N
N
Krishnamoorthy
R
R
Goullet
P
P
D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D066298:In Vitro Techniques; D009693:Nucleic Acid Hybridization / Q000502:physiology; D011110:Polymorphism, Genetic / Q000235:genetics*; D011532:Providencia / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D015183:Restriction Mapping
D004269:DNA, Bacterial; D004275:DNA, Ribosomal