title: Endothelin-1 secretion by human fibroblasts in culture: effects of cell density and IFN-beta.
abstract: Evidence is presented of ET-1 release by cultured human fibroblasts. Such a conclusion is supported by the parallelism in displacement curves obtained using dilutions of extracts prepared from fibroblast conditioned media and the synthetic ET-1 standards, and the significant time dependent increase in ET-1 content in media from IFN-beta treated human fibroblasts. An increase in cell density significantly elevated the total amount of ET-1 in the conditioned media, although a linear relationship between these two variables was not observed.
authors.affiliation: Department of Physiology, New York Medical College, Valhalla 10595.
authors.forename: G A
authors.initials: GA
authors.lastname: Zeballos
authors.forename: S J
authors.initials: SJ
authors.lastname: An
authors.forename: J M
authors.initials: JM
authors.lastname: Wu
mesh_terms: D002452:Cell Count; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D016232:Endothelins / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D016899:Interferon-beta / Q000494:pharmacology; D013268:Stimulation, Chemical
chemical_list: D003470:Culture Media; D016232:Endothelins; D016899:Interferon-beta