Quantitation of trihexyphenidyl from plasma using a mass-selective detector and electron-impact ionization.
A method is described for the measurement of plasma concentrations of trihexyphenidyl, an anti-parkinsonian drug. The drug was extracted from human plasma samples. Then, gas chromatography-mass spectrometry with electron-impact ionization and selected-ion monitoring allowed the specific quantitation of trihexyphenidyl, with bupivacaine used as an internal standard. Linear calibration curves were obtained in the concentration range 5-100 ng/ml. Precision and accuracy were found acceptable for quantitation during pharmacokinetic trials of the drug. This method has been successfully applied to bioavailability studies after Parkinane and Artane administration to humans.
Desage
M
M
L.E.A.C.M., Institut des Sciences Pharmaceutiques et Biologiques de Lyon, France.
Rousseau-Tsangaris
M
M
Lecompte
D
D
Brazier
J L
JL
D002045:Bupivacaine; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D014282:Trihexyphenidyl / Q000097:blood* / Q000493:pharmacokinetics
D014282:Trihexyphenidyl; D002045:Bupivacaine