[Fractionation of eukaryotic DNA in a pulsed electrical field. II. Discrete DNA fragments and level of structural organization of chromatin].
The data presented confirm the possibility of enzymatic formation of discrete DNA-fragments appearing during fractionation of nuclear DNA by FIGE. Teniposide-dependent pattern of DNA-fragments as well as occurrence of protein-linked DNA breaks suggest that discrete cleavage of intact nuclear DNA is modulated by DNA topoisomerase II. The possible relationship between discrete DNA-fragments and the higher order chromatin folding are discussed.
Solov'ian
V T
VT
Andreev
I O
IO
Kunakh
V A
VA
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent; D002843:Chromatin; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D016521:Electrophoresis, Gel, Pulsed-Field; D005057:Eukaryotic Cells / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D051381:Rats; D013713:Teniposide / Q000494:pharmacology
D002413:Cations, Divalent; D002843:Chromatin; D004247:DNA; D013713:Teniposide; D004492:Edetic Acid; D008274:Magnesium; D002118:Calcium
Fraktsionirovanie DNK éukariot v pul'siruiushchem élektricheskom pole. II. Diskretnye fragmenty DNK i urovni strukturnoĭ organizatsii khromatina.