Purification of serum albumin with dye-ligand adsorption chromatography.
Dye molecules were coupled to agarose gel beads to form blue, red and yellow chromatography media for the separation of serum albumin. The adsorption properties of five dye-ligands were compared and the effects of temperature, pH, protein concentration and salt concentration were investigated. Human serum albumin (HSA) was purified from human placental plasma by a single step of dye-ligand chromatography under suitable operating conditions. The product was electrophoretically pure, and the elution recovery was as high as 95%. This new technique is superior to the conventional salting-out process.
Su
Z G
ZG
Dalian University of Technology.
Jiang
W
W
Feng
P S
PS
Gao
K Y
KY
D000327:Adsorption; D002847:Chromatography, Agarose / Q000379:methods*; D004396:Coloring Agents; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D015394:Molecular Structure; D012709:Serum Albumin / Q000302:isolation & purification*; D013696:Temperature
D004396:Coloring Agents; D008024:Ligands; D012709:Serum Albumin