[Isolation and characteristics of ligand specificity of VLA-1 integrin from human smooth muscles].
Using affinity chromatography with immobilized monoclonal antibodies to the beta 1-subunit of human integrin, a total integrin fraction (subfamily beta 1) was isolated from the detergent extract of human smooth muscle (uterus). Immunoprecipitation and immunoblotting with specific antibodies revealed integrins VLA-1 and VLA-5. The former was isolated in a homogeneous state by chromatography on immobilized type I collagen in the presence of 1 mM Mn2+. The pure receptor yield was 2-4 mg per 400 g of smooth muscle tissue. Analysis of substrate specificity of VLA-1 in the liposome test revealed that this integrin possesses a broad spectrum of ligand specificity and can interact via a Ca2+, Mg(2+)-dependent mechanism with interstitial collagens of I, II and III types and with basal membrane proteins (type IV collagen and laminin). VLA-1 does not interact with fibronectin, thrombospondin or albumin. Denaturation of type I collagen decreases the liposome binding 5-7-fold. The peptide Gly-Arg-Gly-Asp-Ser-Pro added to the incubation mixture does not inhibit the liposome interaction with incorporated VLA-1 integrin, type I collagen and laminin.
Belkin
V M
VM
Belkin
A M
AM
Dol'nikova
A E
AE
Tarasova
E A
EA
Kotelianskiĭ
V E
VE
D000595:Amino Acid Sequence; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D002413:Cations, Divalent; D002846:Chromatography, Affinity; D003094:Collagen / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D008024:Ligands; D008247:Lysosomes / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism; D011233:Precipitin Tests; D016029:Receptors, Very Late Antigen / Q000378:metabolism*; D013379:Substrate Specificity
D002413:Cations, Divalent; D016023:Integrins; D008024:Ligands; D009842:Oligopeptides; D016029:Receptors, Very Late Antigen; D003094:Collagen; D008274:Magnesium; D002118:Calcium
Vydelenie i kharakteristika ligandnoĭ spetsifichnosti VLA-1 integrina iz gladkikh myshts cheloveka.