Potentiality of an organometallic labelled streptavidin--biotin system in metalloimmunoassay.
Biotin labelled with a cymantrene moiety (cyclopentadienyl manganese tricarbonyl complex) is described for the first time. Because this metallo-biotin retains full recognition for the specific glycoprotein avidin (or streptavidin), the labelled streptavidin-biotin system is proposed for use in a solid-phase competition-type metalloimmunoassay in which bovine serum albumin (BSA) is used as a model of applications. Atomic absorption spectrometry is utilized for the detection of the cymantrene-labelled biotin.
Rémy
I
I
Unité d'Immunoanalyse, Université de Bourgogne, Dijon, France.
Brossier
P
P
Lavastre
I
I
Besançon
J
J
Moïse
C
C
D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry; D001710:Biotin / Q000032:analysis* / Q000737:chemistry; D007118:Immunoassay / Q000379:methods; D008345:Manganese / Q000737:chemistry; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry; D013054:Spectrophotometry, Atomic; D019809:Streptavidin
D001426:Bacterial Proteins; D009942:Organometallic Compounds; D008345:Manganese; D001710:Biotin; D019809:Streptavidin; C044679:manganese cyclopentadienyl tricarbonyl