Solid-phase peptide synthesis without side-chain hydroxyl protection of threonine.
A peptide containing four threonine residues was synthesised by the solid-phase method using fluorenyl-methoxycarbonylamino acid reactive esters or coupling by preactivation with 1-hydroxybenzotriazole and Castro's reagent. In two separate experiments the synthesis was carried out with or without protection of the side-chain hydroxyl group of threonine as the tert.-butyl ether. Comparison of the crude peptides after deprotection and detachment from the synthesis resin suggests that side-chain protection of threonine is unnecessary under the synthetic conditions employed.
Fischer
P M
PM
Deakin Research Ltd., University of Western Sydney, Richmond, NSW, Australia.
Retson
K V
KV
Tyler
M I
MI
Howden
M E
ME
D009842:Oligopeptides / Q000737:chemistry*; D013912:Threonine / Q000737:chemistry*
D009842:Oligopeptides; D013912:Threonine