Involvement of wild-type p53 protein in the cell cycle requires nuclear localization.
Transfection of wild-type p53 into a pre-B, p53 nonproducer cell line yielded the generation of stable clones. Although constitutively expressing the growth-suppressor wild-type p53 protein, these cells proliferate continuously in vitro. However, expression of wild-type p53 in these cells altered their cell cycle pattern and reduced their growth in vivo. When the same parental cells were transfected with a plasmid coding for a wild-type p53 lacking nuclear localization signals, a wild-type cytoplasmic p53 protein was expressed. Expression of this cytoplasmic p53 product did not exert any changes in the growth of the parental cells, suggesting that wild-type p53 affects the cell cycle only when localized in the nuclear cell compartment.
Shaulsky
G
G
Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Goldfinger
N
N
Peled
A
A
Rotter
V
V
D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002458:Cell Fractionation; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation / Q000502:physiology; D009374:Neoplasms, Experimental / Q000503:physiopathology*; D011233:Precipitin Tests; D014162:Transfection / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000502:physiology*
D016159:Tumor Suppressor Protein p53