title: Determination of the amino acid sequence of cystine-containing peptides by tandem mass spectrometry.
abstract: A method has been developed for the determination of the amino-acid sequence of a cyclic peptide containing cystine. It is based on the reduction of the peptide in a reductive matrix prior to ionization by fast-atom bombardment. The amino-acid sequence of the resulting linear peptide is then determined by tandem mass spectrometry from the spectrum produced by the collision-induced decomposition of the [M + H]+ ion of the peptide.
authors.affiliation: Department of Chemistry, University of Warwick, Coventry, UK.
authors.forename: D
authors.initials: D
authors.lastname: Despeyroux
authors.forename: J
authors.initials: J
authors.lastname: Bordas-Nagy
authors.forename: K R
authors.initials: KR
authors.lastname: Jennings
mesh_terms: D000595:Amino Acid Sequence; D001127:Arginine Vasopressin / Q000032:analysis*; D008236:Lypressin / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010121:Oxytocin / Q000031:analogs & derivatives* / Q000032:analysis*
chemical_list: D001127:Arginine Vasopressin; C100032:tocinoic acid; D010121:Oxytocin; D008236:Lypressin