Platelet-activating factor may mediate dexamethasone-induced gastric damage in the rat.
The possible role of platelet-activating factor (PAF) in dexamethasone-induced gastric mucosal damage was studied in rats. PAF was measured by a platelet aggregation assay. The identity of the PAF-like product recovered from gastric tissues was ascertained by thin-layer chromatography and high-pressure liquid chromatography. Low levels of PAF were detected in the normal rat stomach, while in dexamethasone-treated animals PAF levels were significantly higher. Pretreatment of the animals with BN 52021, a specific PAF receptor antagonist, significantly attenuated dexamethasone-induced mucosal injury. These findings suggest that PAF may be a mediator of mucosal damage induced by glucocorticoids.
Filep
J
J
Department of Pathophysiology, Semmelweis University Medical School, Budapest, Hungary.
Hermán
F
F
Braquet
P
P
D000818:Animals; D002855:Chromatography, Thin Layer; D003907:Dexamethasone / Q000633:toxicity*; D004224:Diterpenes; D005753:Gastric Mucosa / Q000187:drug effects* / Q000473:pathology; D046934:Ginkgolides; D007783:Lactones / Q000494:pharmacology*; D008297:Male; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000187:drug effects / Q000502:physiology*
D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D010972:Platelet Activating Factor; D003907:Dexamethasone; C045856:ginkgolide B