Multiparameter optimizations in micellar liquid chromatography using the iterative regression optimization strategy.
An extension of the iterative regression optimization strategy to multi-parameter optimizations is described and applied to the separation of ionic compounds (amino acids and peptides) by means of micellar liquid chromatography. The parameters examined are the concentration of surfactant, the concentration of 2-propanol and pH. Fairly regular (linear, weakly curved) retention behaviour of the compounds as a function of the parameters results in an efficient optimization using a relatively small number of initial experiments.
Strasters
J K
JK
North Carolina State University, Department of Chemistry, Raleigh 27695.
Kim
S T
ST
Khaledi
M G
MG
D000596:Amino Acids / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D010455:Peptides / Q000032:analysis
D000596:Amino Acids; D008823:Micelles; D010455:Peptides