Analysis of carbamazepine and its 10,11-epoxide in serum by direct sample injection using surfactant containing eluents and column switching.
Column switching is used in conjunction with surfactant containing mobile phases and traditional reverse phase LC columns to provide a highly reproducible and accurate analytical procedure for carbamazepine and its 10,11-epoxide in serum. This approach eliminates the tedious sample preparation steps commonly used in the analysis of drugs by HPLC, while providing a high degree of protein removal prior to the final analysis. Various pre-columns were investigated and a pellicular reverse phase column was found suitable for optimum concentration of drugs and removal of serum proteins. A variety of standard reverse phase columns could be used for the analytical separation. The separation of the drugs could be accomplished with a high degree of reproducibility. Tandem pre-column operation was demonstrated to give a sample throughput of 10 h-1.
Schmitz
S
S
Waters Chromatography Division of Millipore Corporation, Milford, MA 01757.
Warren
F V
FV
Bidlingmeyer
B A
BA
D002220:Carbamazepine / Q000031:analogs & derivatives* / Q000097:blood*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D016903:Drug Monitoring / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D013501:Surface-Active Agents / Q000032:analysis
D013501:Surface-Active Agents; D002220:Carbamazepine; C012227:carbamazepine epoxide