Nonuniform size distribution of nascent globin peptides, evidence for pause localization sites, and a contranslational protein-folding model.
Examination of nascent globin peptides accumulating in vitro during globin synthesis in rabbit reticulocyte lysates was carried out. A view was supported that nonrandom distribution of codons with different usage frequencies in mRNA may determine the messenger's translation kinetics. Regions of reduced translation of alpha- and beta-globin polypeptide chains were localized, and the cotranslational protein-folding model suggested previously was substantiated. An active conjunction of synthesis and folding of proteins was proposed as one of the main destinations of a translation nonuniformity.
Krasheninnikov
I A
IA
Department of Molecular Biology, Faculty of Biology, Moscow State University, USSR.
Komar
A A
AA
Adzhubei
I A
IA
D000818:Animals; D003062:Codon; D005914:Globins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D015394:Molecular Structure; D014176:Protein Biosynthesis; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
D003062:Codon; D012333:RNA, Messenger; D005914:Globins