Polyclonal antibodies as probes to distinguish between tight and loose couple 50S ribosomes of Escherichia coli.
Antibody has been raised in rabbit against L7/L12 protein of E. coli 50S ribosomes and purified, finally through affinity column. A sensitive assay method using ELISA technique has also been standardised. LC 50S ribosomes react more with the antibody than TC 50S ribosomes. This supports the earlier physical data [Burma D P, Srivastava A K, Srivastava S, Tewari D S, Dash D & Sengupta S K, (1984), Biochem Biophys Res Commun, 124, 970] indicating that L7/L12 stalk region is protruded in medium in LC ribosomes and folded towards the body in TC ribosomes.
Dey
D N
DN
Molecular Biology Unit, Banaras Hindu University, Varanasi.
Burma
D P
DP
D000907:Antibodies, Bacterial; D004926:Escherichia coli / Q000737:chemistry / Q000276:immunology; D029968:Escherichia coli Proteins; D015335:Molecular Probes; D012269:Ribosomal Proteins / Q000737:chemistry / Q000276:immunology; D012270:Ribosomes / Q000737:chemistry / Q000276:immunology*
D000907:Antibodies, Bacterial; D029968:Escherichia coli Proteins; D015335:Molecular Probes; D012269:Ribosomal Proteins; C512844:rplL protein, E coli; C016700:ribosomal protein L7-L12