title: Simultaneous uptake of galactose and glucose by Azotobacter vinelandii.
abstract: Azotobacter vinelandii growing on galactosides induced two distinct permeases for glucose and galactose. The apparent Vmax and Km of the galactose permease were 16 nmol galactose/min per 10(10) cells and 0.5 mM, respectively. The apparent Vmax and Km of the glucose permease were 7.8 nmol glucose/min per 10(10) cells and 0.04 mM, respectively. Excess glucose had no effect on the galactose uptake. However, excess galactose inhibited glucose transport. The galactosides-induced glucose permease also exhibited different uptake kinetics from that induced by glucose.
authors.affiliation: Biology Department, Memphis State University, TN 38152.
authors.forename: T Y
authors.initials: TY
authors.lastname: Wong
authors.forename: C A
authors.initials: CA
authors.lastname: Murdock
authors.forename: S P
authors.initials: SP
authors.lastname: Concannon
authors.forename: T D
authors.initials: TD
authors.lastname: Lockey
mesh_terms: D016948:Azotobacter vinelandii / Q000378:metabolism*; D004790:Enzyme Induction; D005690:Galactose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D008553:Melibiose / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism
chemical_list: D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; C043910:galactose permease; D008553:Melibiose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C031629:phosphoenolpyruvate-glucose phosphotransferase; D005947:Glucose; D005690:Galactose