title: Sequence and transcriptional analysis of the gene cluster trnQ-zfpA-psaI-ORF231-petA in pea chloroplasts.
abstract: A 5.1 kb segment of pea chloroplast DNA containing the upstream region of petA was sequenced. RNAs produced from this DNA were characterized. This region encodes putative genes for psbK, trnQ, zfpA, psaI, ORF231, and petA. These genes are all on the same reading strand except for psbK. The gene organization is somewhat different from that of tobacco, rice, and liverwort, which lack the psbK-trnQ genes in this region and contain ORF184/185. Northern blot and primer extension analysis show that the pea transcript covers the zfpA-psaI-ORF231-petA gene cluster and trnQ. These results indicated that the psbK-trnQ genes have been rearranged and a new transcription unit was formed.
authors.affiliation: Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nagano
authors.forename: R
authors.initials: R
authors.lastname: Matsuno
authors.forename: Y
authors.initials: Y
authors.lastname: Sasaki
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002736:Chloroplasts; D004247:DNA; D018742:DNA, Chloroplast; D007887:Fabaceae / Q000235:genetics*; D015321:Gene Rearrangement; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D009876:Operon; D010940:Plant Proteins / Q000235:genetics; D010946:Plants, Medicinal; D014158:Transcription, Genetic
chemical_list: D018742:DNA, Chloroplast; D010940:Plant Proteins; C074138:zfpA protein, Pisum sativum; D004247:DNA