Use of monoclonal antibodies against human T cells for clinical diagnosis by immunohistochemical procedures.
Monoclonal antibodies (Mabs) were produced against human T cell membrane antigens. Sixteen Mabs were studied and six were selected for immunohistochemical assays on paraffin-embedded tonsil sections. Two Mabs (2D7 and 1E2) specifically recognized T-lymphocyte areas in sections of pathological tissues originating from lymphoproliferative diseases, and reacted with proteins of approximately 80 kDa. Most of the Mabs produced thus far are only suitable for immunohistochemical assays on frozen section. Only a few Mabs recognize lymphoid markers on paraffin-embedded sections, a procedure which permits a more extensive and practical application of Mabs in clinical diagnosis. These antibodies should be valuable in diagnosing T cell-related diseases and their large scale production should reduce laboratory costs because all reagents currently available are imported.
Duarte
A J
AJ
Laboratório de Imunogenética e Transplante Experimental, Faculdade de Medicina, Universidade de São Paulo, Brasil.
Sato
M N
MN
Carneiro
C
C
Figueiredo
C A
CA
Blanco
S A
SA
Santos
R T
RT
Alves
V A
VA
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D015153:Blotting, Western; D006801:Humans; D007150:Immunohistochemistry; D007938:Leukemia / Q000175:diagnosis*; D008223:Lymphoma / Q000175:diagnosis*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface