The influence of D-Ala1-peptide T amide, an analogue of HIV glycoprotein 120 fragment, on the CD4--anti CD4 lymphocyte interaction.
D-Ala1-peptide T amide (DAPTA), synthetic analogue of the HIV glycoprotein 120 sequence, was used to study its binding to specific cellular receptor of HIV, CD4. The analogue contains eight aminoacid residues including 4 threonine residues; its molecular weight being 992. We have shown the temperature- and dose-dependent inhibitory effect of peptide T on the CD4 - anti CD4 binding. The strongest effect was noted at 37 degrees C with the peptide dose of 150 nM: 62% inhibition of binding. Other means of possible blocking of HIV attachment to the host cellular receptor are discussed.
Walczak
M
M
Department of Immunology, Children's Memorial Hospital, Warszawa.
Imielska
D
D
Maćkiewicz
Z
Z
Kupryszewski
G
G
Dzierzanowska-Madalińska
D
D
Madaliński
K
K
D000595:Amino Acid Sequence; D000937:Antigen-Antibody Reactions / Q000187:drug effects; D001665:Binding Sites; D001667:Binding, Competitive; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D015717:Peptide T / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D015728:Receptors, HIV / Q000187:drug effects* / Q000378:metabolism
D015699:HIV Envelope Protein gp120; D015728:Receptors, HIV; D015717:Peptide T; C055175:peptide T amide