title: Bilirubin conjugation and biliary bilirubin excretion after intravenous bilirubin injection in dogs.
abstract: This study was undertaken to investigate the bilirubin clearance, bilirubin conjugation and biliary bilirubin excretion after an intravenous single bilirubin injection (5 mg/kg or 10 mg/kg) in 10 mongrel dogs. Serum total bilirubin concentration increased after the bilirubin injection, and then it immediately decreased. Biliary excretion of loaded bilirubin induced a rapid increase of the bile total bilirubin concentration and bilirubin-monoconjugate. In the 10 mg/kg group, the increase was larger than in the 5 mg/kg group and remained high up to 4 hr after injection, whereas in the 5 mg/kg group these bile components tended to decrease and returned to the preinjection levels within 4 hr. The total bile acid concentration in the bile remained unchanged in both groups. The bile flow did not change in the 5 mg/kg group, but in the 10 mg/kg group it decreased after bilirubin loading. Significantly positive correlations were observed between total bilirubin and bilirubin monoconjugate concentrations in bile both before and 1 hr after injection, but the slopes of regression lines were markedly different from each other. These results suggest that a bilirubin load surpassing the conjugating capacity of the hepatocytes increases bile bilirubin monoconjugate and also enhances the conjugating activity in the liver.
authors.affiliation: First Department of Surgery, Tohoku University School of Medicine, Sendai.
authors.forename: R
authors.initials: R
authors.lastname: Usui
authors.forename: H
authors.initials: H
authors.lastname: Ise
authors.forename: O
authors.initials: O
authors.lastname: Kitayama
authors.forename: A
authors.initials: A
authors.lastname: Moriyasu
authors.forename: Y
authors.initials: Y
authors.lastname: Abe
authors.forename: S
authors.initials: S
authors.lastname: Satoh
authors.forename: H
authors.initials: H
authors.lastname: Inoue
authors.forename: H
authors.initials: H
authors.lastname: Hayasaka
authors.forename: Y
authors.initials: Y
authors.lastname: Hirama
authors.forename: H
authors.initials: H
authors.lastname: Honda
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism / Q000502:physiology; D001647:Bile Acids and Salts / Q000378:metabolism; D001663:Bilirubin / Q000008:administration & dosage / Q000378:metabolism* / Q000493:pharmacokinetics; D004285:Dogs; D007275:Injections, Intravenous
chemical_list: D001647:Bile Acids and Salts; D001663:Bilirubin