[Macrophage activation by synthetic peptides. III. Changes in the membrane potential, Ca2+ content and the regulatory decrease of macrophage volume under the action of tuftsin and its antagonist].
With the use of oxonol voltage-sensitive fluorescent dye it has been shown that the stimulation of macrophages (MP) with tuftsin results in a two-phase change in membrane potential: depolarization followed by hyperpolarization of plasma membrane. The pattern of changes in membrane potential depends on Na+ concentration in the medium and is disturbed with binding of cytoplasmic Ca2+. Fluorescent signal obtained from MP loaded with Ca(2+)-activated photoprotein obelin points to a significant increase in the concentration of cytoplasmic Ca2+ under the influence of tuftsin on cells: the source for Ca2+ being the medium. The rate of regulatory voltage decrease in MP increases under the influence of tuftsin: the effect of this peptide being similar to that of calcium ionophore. All these findings taken together enable us to suggest a phenomenological scheme of transmembrane ion signals arising during stimulation of MP with tuftsin: the receptor-mediated calcium channel provides a rise in cytoplasmic Ca2+ which opens non-selective cation channels for Na+ ions to activate eventually Ca(2+)-dependent K(+)-transport.
Gamaleĭ
I A
IA
Kaulin
A B
AB
Kirpichnikova
K M
KM
D000818:Animals; D001463:Barbiturates; D002118:Calcium / Q000378:metabolism*; D002448:Cell Adhesion; D005456:Fluorescent Dyes; D036341:Intercellular Signaling Peptides and Proteins; D007555:Isoxazoles; D008027:Light; D008164:Luminescent Proteins / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects* / Q000502:physiology; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000494:pharmacology*; D012542:Scattering, Radiation; D014405:Tuftsin / Q000037:antagonists & inhibitors* / Q000494:pharmacology*
D001463:Barbiturates; D005456:Fluorescent Dyes; D036341:Intercellular Signaling Peptides and Proteins; D007555:Isoxazoles; D008164:Luminescent Proteins; D010455:Peptides; C010497:obelin; C018622:oxonol dyes (isoxazole); C052795:bis(1,3-dibutylbarbiturate)trimethine oxonol; C076284:peptide KPR; D014405:Tuftsin; D002118:Calcium
Aktivatsiia makrofagov sinteticheskimi peptidami. III. Izmenenie membrannogo potentsiala, soderzhaniia Ca2+ i reguliatornoe umen'shenie ob''ema makrofagov pri deĭstvii taftsina i ego antagonista.