[Metabolic differentiation of peripheral blood lymphocytes after kidney transplantation in relation to post-transplantation immuno- suppression in the light of the analysis of cellular activity of DNA transcription enzymes].
The activity of three classes DNA-dependent RNA polymerases in T and B lymphocyte cells nuclei isolated from peripheral blood of patients with transplanted kidney were investigated. Twenty three patients with transplanted organ in age 35 +/- 9.7 treated simultaneously with cyclosporin A and small doses of prednisone and thirteen persons after renal transplantation in age 34.8 +/- 6.3 treated conservatively (azathioprine plus prednisone) were studied. The enzymes activity was assayed by the measurement of [3H] UTP incorporated into acid insoluble product in the presence of alpha-amanitin when specified. Both, "bound" and "free" enzymes activity was analysed. "Bound" polymerase is defined by the ability to transcribe endogenous template in the absence of exogenous DNA. "Free" enzyme was determined by the additional transcription on exogenous, calf thymus DNA as template. The quantity of polymerase I subunits by Western blotting was also analysed. It was shown that the polymerizing enzymes activity strongly depend upon haemodialysis period of time prior to organ transplantation as well as upon the treatment after transplantation. Characteristically in case of T lymphocyte isolated from patients treated with cyclosporin A, the transplant rejection process was accompanied by large increasing in polymerase activity especially in polymerase I. The correlation in polymerase activity and transplant rejection time was clearly observed. In case of lymphocyte cells isolated from patients with renal transplant treated conservatively, the polymerase activity in both T and B cell was slightly reduced. The useful of polymerase assay for monitoring of patients with renal transplant is considered.
Adamiec
R
R
Katedra i Klinika Nefrologii Akademii Medycznej we Wrocławiu.
Szewczyk
Z
Z
Szopa
J
J
D000328:Adult; D001379:Azathioprine / Q000008:administration & dosage*; D001402:B-Lymphocytes / Q000187:drug effects / Q000201:enzymology*; D016572:Cyclosporine / Q000008:administration & dosage*; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors / Q000097:blood*; D005260:Female; D006084:Graft Rejection / Q000187:drug effects / Q000276:immunology; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011182:Postoperative Care; D011241:Prednisone / Q000008:administration & dosage*; D011379:Prognosis; D013601:T-Lymphocytes / Q000187:drug effects / Q000201:enzymology*
D016572:Cyclosporine; D012321:DNA-Directed RNA Polymerases; D001379:Azathioprine; D011241:Prednisone
Zróznicowanie metaboliczne limfocytów krwi obwodowej chorych po przeszczepieniu nerek w zalezności od zastosowanej immunosupresji potransplantacyjnej w świetle analizy komórkowej aktywności enzymów transkrypcyjnych DNA.