Isolation and characterization of glycocalyx in Streptomyces aureofaciens.
The surface layer, considered to be glycocalyx according to electron-microscopic observations, was separated from a low-production strain of Streptomyces aureofaciens by solubilization with urea and subsequent sonication. The isolation procedure was developed using various agents; neutral phosphatase served as a marker indicating the amount of the material released. The peripheral structure consisted predominantly of glycoprotein and differed from S-layers.
Shabalin
J
J
Institute of Biochemistry and Physiology of Microorganisms, Pushchino, Moscow Region, USSR.
Benes
I
I
Hostálek
Z
Z
Kulayev
I
I
D000596:Amino Acids / Q000032:analysis; D002458:Cell Fractionation; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D013304:Streptomyces aureofaciens / Q000737:chemistry*
D000596:Amino Acids; D006023:Glycoproteins; D011134:Polysaccharides