title: [Electron microscopic study of changes in chromosomal structural organization under the effect of formamide].
abstract: Isolated human metaphase chromosomes were treated with formamide at different (0-70%) concentrations and examined electronmicroscopically by protein monolayer technique. At increasing formamide concentration chromosomes gradually decondense, the scaffold becomes more clearly visible, the loops of chromatin fibres coming off the central part of chromosomes lose their nucleosomal appearance. Electrophoretic analysis of chromosomal proteins data show that formamide-treated chromosomes have approximately the same histone content as those before treatment, although chromosomes treated with 70% formamide look very similar to histone-depleted ones described elsewhere.
authors.forename: E G
authors.initials: EG
authors.lastname: Novikova
authors.forename: L P
authors.initials: LP
authors.lastname: Martynikina
authors.forename: T V
authors.initials: TV
authors.lastname: Nasedkina
authors.forename: S I
authors.initials: SI
authors.lastname: Slezinger
authors.forename: G V
authors.initials: GV
authors.lastname: Serdiukov
authors.forename: V I
authors.initials: VI
authors.lastname: Popenko
mesh_terms: D002877:Chromosomes, Human / Q000187:drug effects / Q000648:ultrastructure*; D004591:Electrophoresis, Polyacrylamide Gel; D005559:Formamides / Q000494:pharmacology*; D006657:Histones / Q000032:analysis; D006801:Humans; D008854:Microscopy, Electron
chemical_list: D005559:Formamides; D006657:Histones; C031066:formamide
vernacular_title: Elektronno-mikroskopicheskoe issledovanie izmeneniĭ strukturnoĭ organizatsii khromosom pod deĭstviem formamida.