DNAase activities in embryonic chicken lens: in epithelial cells or in differentiating fibers where chromatin is progressively cleaved.
Lens is an organ composed of a layer of epithelial cells and a mass of fibers. During terminal differentiation, epithelial cells from the equatorial region elongate into fibers, nuclei change shape, the chromatin appears much condensed in the last step of differentiation and the DNA breaks down into nucleosomes. The pattern of DNAase activities has been recorded at different chick embryonic stages (11 and 18 days) using polyacrylamide gel electrophoresis with DNA substrate in the gel matrix. Two DNAases (30 and 40 kDa) have been observed in lens epithelia and fibers at both stages. However, the activities of both of the enzymes are augmented in fiber cells. The 30 kDa DNAase requires and Ca2+ and Mg2+ (5-15 mM) to hydrolyze the DNA substrate while the 40 kDa-activity is inhibited by added divalent cations (5-15 mM). The 30 kDa protein is inhibited by Na+ and is probably an endonuclease. Both nuclease activities probably are involved in the cleavage of fiber chromatin into nucleosomes during lens terminal differentiation, but variables such as chromatin configuration, unmasked DNA sequences, presence of cations, and pH gradients probably determine the extent of involvement of each DNAase.
Counis
M F
MF
U118 INSERM, Association Claude Bernard, Paris, France.
Chaudun
E
E
Courtois
Y
Y
Allinquant
B
B
D000818:Animals; D002454:Cell Differentiation; D002642:Chick Embryo; D002843:Chromatin / Q000502:physiology* / Q000648:ultrastructure; D003851:Deoxyribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors / Q000032:analysis / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000201:enzymology; D007908:Lens, Crystalline / Q000166:cytology / Q000196:embryology / Q000201:enzymology*; D008970:Molecular Weight; D014357:Trypsin
D002843:Chromatin; D004792:Enzyme Precursors; D003851:Deoxyribonucleases; D014357:Trypsin