[Role of creatine phosphokinase in the energy supply of the pumping function of the heart].
The cardiac output of isolated working rat heart and left ventricular pressure were estimated in either almost complete inhibition of creatine kinase by iodoacetamide or predominant fall in adenine nucleotides (AdN) content induced by 2-deoxyglucose treatment. In the former case, a profound cardiac pump failure was observed despite almost normal levels of myocardial AdN and phosphocreatine. Those hearts could not maintain the aortic output at standard load due to lower LV systolic pressure, that was accompanied by increased minimal and maximal diastolic pressures by 5-7 mm Hg as well as by LV diastolic stiffness. As LV systolic pressure in those hearts was unchanged in retrogradely perfused and unloaded hearts it might be suggested that the cardiac pump failure was caused by the decreased LV distensibility. On the contrary, deoxyglucose treatment that resulted in 70% fall in the AdN content was accompanied by only moderate reduction of the cardiac output and insignificant changes in LV diastolic pressure and stiffness. The results suggested that creatine kinase plays a crucial role in the maintenance of normal myofibrillar compliance, which is necessary for cardiac filling and pump function.
Kapel'ko
V I
VI
Novikova
N A
NA
Kupriianov
V V
VV
Sake
V A
VA
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002303:Cardiac Output, Low / Q000139:chemically induced; D003402:Creatine Kinase / Q000037:antagonists & inhibitors / Q000502:physiology*; D003847:Deoxyglucose / Q000494:pharmacology*; D004734:Energy Metabolism / Q000502:physiology*; D066298:In Vitro Techniques; D007460:Iodoacetamide / Q000494:pharmacology*; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000201:enzymology*; D051381:Rats
D000227:Adenine Nucleotides; D003847:Deoxyglucose; D003402:Creatine Kinase; D007460:Iodoacetamide
Rol' kreatinfosfokinazy v énergoobespechenii nasosnoĭ funktsii serdtsa.