title: Euro-Collins solution versus UW-solution for long-term liver preservation in the isolated rat-liver perfusion model.
abstract: To compare UW-solution (UW) and Euro-Collins (EC) for long-term liver preservation we investigated the morphology and metabolic capacity of rat liver after 18 and 42-hours cold-storage in either UW or EC. After harvesting the rat liver was transferred to a perfusion chamber where it was perfused for 10 min with UW or EC at 4 degrees C. Thereafter livers were stored at 4 degrees C in UW or EC for 18 hours (both groups n = 6) or for 42 hours (both groups n = 8). After 18-hr or 42-hr cold-storage a 2-hr warm perfusion (37 degrees C) was started with Krebs-Ringer solution with carbogen to which 125Iodine-triiodothyronine (T3) was added. Control livers (n = 8) were immediately perfused with Krebs-Ringer without cold-storage. The following parameters were assessed: ASAT-levels in the perfusate, T3-metabolites in the bile and the perfusate, the perfusion pressure, the volume of bile secreted and light-microscopical morphology at the end of the warm perfusion period. After cold storage in UW-solution the ASAT-levels in the perfusate were lower than after storage in EC as well as the perfusion pressures. These livers demonstrated a better T3-metabolism and secreted more bile than EC-stored livers. Histological examination showed more tissue damage in the EC-stored livers than in the UW stored livers. We conclude that cold-storage of rat liver in UW-solution resulted in a better morphology and metabolic capacity as compared with EC-solution.
authors.affiliation: Department of Surgery, University Hospital Dijkzigt, Erasmus University Rotterdam, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Den Toom
authors.forename: M
authors.initials: M
authors.lastname: De Jong
authors.forename: E P
authors.initials: EP
authors.lastname: Krenning
authors.forename: H J
authors.initials: HJ
authors.lastname: Van der Hoek
authors.forename: F J
authors.initials: FJ
authors.lastname: Ten Kate
authors.forename: G
authors.initials: G
authors.lastname: Hennemann
authors.forename: O T
authors.initials: OT
authors.lastname: Terpstra
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001646:Bile / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D005978:Glutathione; D006982:Hypertonic Solutions / Q000592:standards*; D007328:Insulin; D008099:Liver / Q000033:anatomy & histology* / Q000378:metabolism; D008297:Male; D019188:Organ Preservation Solutions; D011887:Raffinose; D051381:Rats; D011919:Rats, Inbred Strains; D015424:Reperfusion; D012996:Solutions / Q000592:standards*; D014021:Tissue Preservation / Q000379:methods / Q000592:standards*; D014284:Triiodothyronine / Q000378:metabolism / Q000493:pharmacokinetics
chemical_list: C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D014284:Triiodothyronine; D000493:Allopurinol; D001219:Aspartate Aminotransferases; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose