Molecular cloning and transcriptional analysis of the cpeBA operon of the cyanobacterium Pseudanabaena species PCC7409.
The cpeBA operon of the Group III chromatically adapting cyanobacterium Pseudanabaena species PCC 7409 was cloned, sequenced and characterized. The cpeBA genes are transcribed in green-light-grown cells as an abundant 1400-nucleotide mRNA which initiates 69 nucleotides upstream from the cpeB translation start. Extensive sequence identity, extending 70 nucleotides 5' to the transcription start, occurs among cpeBA promoters of Group II and III chromatic adapters. Cell extracts of green-light-grown Calothrix species PCC 7601 contain an activity which specifically binds a restriction fragment containing the Pseudanabanea species PCC 7409 cpeBA promoter. Green-light-dependent cpeBA transcription in Group II and III chromatically adapting cyanobacteria is suggested to be similarly controlled by a transcriptional activator.
Dubbs
J M
JM
Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Bryant
D A
DA
D000595:Amino Acid Sequence; D001483:Base Sequence; D000458:Cyanobacteria / Q000737:chemistry / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D008027:Light; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D010799:Phycoerythrin / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D016364:Reading Frames / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D010799:Phycoerythrin