Schistosoma mansoni: immuno-localization of the calcium binding protein Sm20.
The 20 kDa calcium binding protein of Schistosoma mansoni, Sm20, is expressed in all stages of the life cycle from the cercaria to the adult worm and in the egg, with equal levels of Sm20 present in adult male and female worms. Localization of Sm20 by immuno-electron microscopy using Sm20-specific antisera and the protein A gold technique demonstrated that the majority of Sm20 is expressed in muscle but that it may also be expressed at low levels in the tegument. These results suggest that Sm20 plays a role in the calcium mediated regulation of muscle contraction. However, it is not clear whether Sm20 acts as a reservoir for calcium in muscle or is directly involved in the regulatory mechanisms of contraction.
Havercroft
J C
JC
Department of Pathology, University of Cambridge, UK.
Smith
A L
AL
Williams
R H
RH
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000032:analysis; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015801:Helminth Proteins / Q000032:analysis*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D016253:Microscopy, Immunoelectron; D011817:Rabbits; D012550:Schistosoma mansoni / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D002135:Calcium-Binding Proteins; D015801:Helminth Proteins; C074267:Sm20 protein, Schistosoma mansoni