Cell-free translation of American hop latent virus RNA.
The RNA of American hop latent virus (AHLV) has a molecular size of 7.7 kb measured in agarose gels. Translation products of AHLV RNA in rabbit reticulocyte and wheat-germ cell-free systems ranged in size up to 200 kD. Time-course experiments indicated that a 36 kD peptide, immunoprecipitated by antivirion sera, was synthesized early followed by the sequential appearance of peptides of increasing Mr. An excess of the amino-acid analogue L-canavanine for arginine specifically reduced the quantity of the 36 kD peptide and induced synthesis of a 38-kD peptide in both rabbit reticulocyte and wheat germ. Translation products were not altered by the addition of a reducing agent, and no product appeared to be produced due to limitation of tRNA species or by readthrough. Synthesis of AHLV RNA-directed peptides was blocked by the cap analogue m7G5'ppp5'G, suggesting the presence of a cap structure at the 5' terminus.
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Botany Department, University of Leicester, United Kingdom.
Mills
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D000818:Animals; D002474:Cell-Free System; D004229:Dithiothreitol / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D006151:Guanosine / Q000031:analogs & derivatives / Q000494:pharmacology; D007700:Kinetics; D010942:Plant Viruses / Q000235:genetics* / Q000302:isolation & purification; D011233:Precipitin Tests; D014176:Protein Biosynthesis / Q000187:drug effects / Q000502:physiology*; D012367:RNA, Viral / Q000235:genetics*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology; D014908:Triticum / Q000378:metabolism
D012367:RNA, Viral; D006151:Guanosine; C016578:7-methylguanosine; D013096:Spermine; D004229:Dithiothreitol; D013095:Spermidine