[Short and long latent effects of 15-hydroxyeicosatetraenoic acid on the extinction of the acetylcholine-induced inward current in neurons of the Helix lucorum].
The impact of a non-cyclized arachidonic acid derivative 15S-hydroxy-5Z, 8Z, 11Z, 13E-eicosatetraenoic acid (15-HETE) upon the dynamics of the inward current extinction, caused by repeated ion-tophoretic acetylcholine applications on the soma, was studied through a double electrode voltage clamp technique on the Helix lucorum identified neurons RPa3 and LPa3. The extracellular effect of 15-HETE (4-16 microM) was found to have a two phase influence on the inward current extinction, depending on the time of exposure to the compound. The short-latent effect (up to 60-80 min) displays itself as an extinction decrease, whereas the long-latent effect (after 60-80 min) - as an enhanced extinction. The effects caused by 15-HETE are irreversible. The short-latent one was probably due to the earlier described inhibition of 5- and 12-lipoxygenase enzymes by 15-HETE while the long-latent one - due to its intrinsic function.
Pivovarov
A S
AS
Drozdova
E I
EI
Belosludtsev
Iu Iu
IuIu
Demin
P M
PM
Miagkova
G I
GI
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D004594:Electrophysiology; D006372:Helix, Snails; D006893:Hydroxyeicosatetraenoic Acids / Q000494:pharmacology*; D066298:In Vitro Techniques; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D011950:Receptors, Cholinergic / Q000187:drug effects / Q000502:physiology; D013997:Time Factors
D006893:Hydroxyeicosatetraenoic Acids; D011950:Receptors, Cholinergic; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D000109:Acetylcholine
Korotko- i dlinnolatentnye éffekty 15-gidroksiéĭkozatetraenovoī kisloty na ugashenie vyzvannogo atsetilkholinom vkhodiashchego toka neĭronov vinogradnoĭ ulitki.