Enhancement of the physicochemical qualities of gastric mucus by sofalcone.
The effect of prolonged administration of an antiulcer drug, sofalcone, on the physicochemical properties of gastric mucus was investigated. The experiments were conducted with groups of rats receiving twice daily for three consecutive days a dose of 100 mg/kg sofalcone, while the control group received daily doses of vehicle. The rats were sacrificed 16 h after the last dose and gastric mucosa subjected to physicochemical measurements. The results revealed that sofalcone evoked a 23% increase in mucus gel dimension, while sulfo- and sialomucins content of the gel increased by 54 and 25%, respectively. These changes were accompanied by a 16% increase in mucus H+ retardation capacity, 2-fold increase in viscosity, and a 39% increase in the gel hydrophobicity. The mucus elaborated in the presence of sofalcone contained 67% more covalently bound fatty acids, exhibited 10% lower content of protein, 30% higher content of carbohydrate, and 18% higher content of lipids. The mucus of the sofalcone group also showed an increase in the proportion of the high molecular weight mucus glycoprotein form, which in the control group accounted for about 30% of gel mucin, while its content in mucus gel of animals receiving sofalcone reached the value of 50%. The results indicate that sofalcone enhances the protective qualities of mucus component of gastric mucosal barrier.
Piotrowski
J
J
Research Center, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
Yamaki
K
K
Tamura
S
S
Slomiany
A
A
Slomiany
B L
BL
D000423:Alcian Blue / Q000493:pharmacokinetics; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002599:Chalcone / Q000031:analogs & derivatives* / Q000494:pharmacology; D047188:Chalcones; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography; D005456:Fluorescent Dyes; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D006023:Glycoproteins / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D008297:Male; D009077:Mucins / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D009093:Mucus / Q000737:chemistry / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D012795:Sialoglycoproteins / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D000897:Anti-Ulcer Agents; D047188:Chalcones; D005456:Fluorescent Dyes; D006023:Glycoproteins; D009077:Mucins; D012795:Sialoglycoproteins; C012564:sulfomucin; C035032:sofalcone; D002599:Chalcone; D000423:Alcian Blue