Application of alcohol- or acetone-fixed Schistosoma eggs as an alternative to lyophilized eggs for the circumoval precipitin test.
Antigenicity of Schistosoma mansoni and S. japonicum eggs preserved in ethanol or acetone were assessed in a circumoval precipitin (COP) assay. The egg antigens were found to retain sufficiently their COP reactivity for the diagnosis of both schistosomiasis mansoni and japonica, although their reactivity became lower than that of lyophilized eggs. These alternative preparations for COP tests have advantages, such as keeping eggs directly in fixatives soon after the egg-purification process. Furthermore, evaporation-process may cause eggshell cleavages which facilitate the reaction. The possible usefulness of those eggs in COP assays in local endemic areas is discussed.
Kamiya
H
H
Department of Parasitology, Hirosaki University School of Medicine, Aomori, Japan.
Liu
P H
PH
Ishigooka
S
S
Blas
B L
BL
D000096:Acetone / Q000592:standards*; D000947:Antigens, Helminth / Q000276:immunology*; D000431:Ethanol / Q000592:standards*; D005069:Evaluation Studies as Topic; D005612:Freeze Drying / Q000592:standards; D006801:Humans; D011233:Precipitin Tests / Q000379:methods* / Q000592:standards; D011309:Preservation, Biological / Q000379:methods* / Q000592:standards; D012554:Schistosomiasis japonica / Q000276:immunology* / Q000469:parasitology; D012555:Schistosomiasis mansoni / Q000276:immunology* / Q000469:parasitology
D000947:Antigens, Helminth; D000096:Acetone; D000431:Ethanol