title: stf1: a new suppressor of the mitotic control gene, cdc25, in Schizosaccharomyces pombe.
abstract: A novel element in the mitotic control, stf1, has been identified genetically by its ability to rescue cdc25-22 as well as a gene disruption of cdc25. This is the first phenotypically non-wee mutation shown to do so. stf1-1 functions additively with cdc2-1w, cdc2-3w, or wee1-6 to rescue cdc25. The available data are consistent with the wild-type gene product operating either on the same pathway as cdc25 or to stimulate cdc2 by a pathway independent of cdc25 or wee1. The stf1 gene has been cloned and sequenced and encodes a putative protein of 50-65 kD, depending on whether a potential intron is present. It is a novel protein with no homology detected in the current data bases. When challenged with hydroxyurea, stf1-1 acts additively with cdc2-3w in rescuing cdc25 mutants and in allowing mitosis to occur without DNA synthesis. It does not appear to play a role in the nutritional sensing pathway nor in the pathway mediating radiation-induced G2 delay.
authors.affiliation: Department of Biology, Queen's University, Kingston, Ontario, Canada.
authors.forename: J D
authors.initials: JD
authors.lastname: Hudson
authors.forename: H
authors.initials: H
authors.lastname: Feilotter
authors.forename: C
authors.initials: C
authors.lastname: Lingner
authors.forename: R
authors.initials: R
authors.lastname: Rowley
authors.forename: P G
authors.initials: PG
authors.lastname: Young
mesh_terms: D000483:Alleles; D018797:Cell Cycle Proteins; D004271:DNA, Fungal / Q000096:biosynthesis / Q000187:drug effects; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006918:Hydroxyurea / Q000494:pharmacology; D008938:Mitosis / Q000235:genetics* / Q000528:radiation effects; D009154:Mutation; D009687:Nuclear Proteins; D011494:Protein Kinases / Q000235:genetics; D011505:Protein-Tyrosine Kinases; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D013489:Suppression, Genetic; D020666:ras-GRF1
chemical_list: D018797:Cell Cycle Proteins; D004271:DNA, Fungal; D005656:Fungal Proteins; D009687:Nuclear Proteins; D029702:Schizosaccharomyces pombe Proteins; D020666:ras-GRF1; D011494:Protein Kinases; C068689:wee1 protein, S pombe; D011505:Protein-Tyrosine Kinases; D006918:Hydroxyurea