title: In vitro combination of high dose busulfan with radiotherapy on medulloblastoma cells: additive effect without potentiation.
abstract: The aim of this in-vitro study was to evaluate the combination of busulfan with radiotherapy on TE-671 human medulloblastoma cells since unexpected clinical toxicity of busulfan was reported during the treatment of brain tumors, suggesting a possible radiopotentiation. The cytotoxicity of busulfan was determined by using colony forming assays, and doses inducing growth inhibitions of 10, 20 and 50% were selected to be tested in association: 6, 12 and 32 mumol/l for busulfan and 0.5, 1 and 3 Gy for irradiation. All possible combinations were considered within this frame and the results showed that the combination of busulfan with radiotherapy exerted an additive effect without potentiation.
authors.affiliation: Centre Alexis Vautrin, Vandoeuvre-Les-Nancy, France.
authors.forename: J L
authors.initials: JL
authors.lastname: Merlin
authors.forename: P
authors.initials: P
authors.lastname: Chastagner
authors.forename: C
authors.initials: C
authors.lastname: Marchal
authors.forename: B
authors.initials: B
authors.lastname: Weber
authors.forename: P
authors.initials: P
authors.lastname: Bey
mesh_terms: D002066:Busulfan / Q000008:administration & dosage*; D002460:Cell Line; D002528:Cerebellar Neoplasms / Q000473:pathology* / Q000628:therapy; D003114:Colony-Forming Units Assay; D003131:Combined Modality Therapy; D006801:Humans; D008527:Medulloblastoma / Q000473:pathology* / Q000628:therapy; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000528:radiation effects
chemical_list: D002066:Busulfan