Anti-HIV-1: extended validation of a modified gelatin particle agglutination assay, and its use with an image analyser.
Modification of a commercial gelatin particle agglutination assay for anti-HIV-1 (MAT) has increased sensitivity, reduced reaction time and lowered the cost by 90%. We tested over 10,000 blood donations in parallel with the ELISA currently in use, and found the modified test to be highly sensitive and to enhance cost-effectiveness as it substantially reduces false-positive rates. The patterns of agglutination are clearly reproducible and readable by the naked eye and/or Image Analyzer, which provides objectivity and hard-copy documentation of results.
Rodriguez
M A
MA
Haematology Dept, Naval Hospital, Vina del Mar, Chile.
Runganga
J
J
Contreras
M
M
Ahya
R
R
Howell
D R
DR
Barbara
J A
JA
D000372:Agglutination Tests / Q000379:methods; D006679:HIV Seropositivity / Q000175:diagnosis*; D015497:HIV-1; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation; D011446:Prospective Studies; D012680:Sensitivity and Specificity