[Effect of antibodies on the catalytic properties of bacillary alpha-amylase].
The interaction between bacillar alpha-amylase and polyclonal antibodies was studied. It was found that the dependence of the enzyme activity on antibody concentration is biphasic, being increased at low concentrations of the antibodies with a further decline down to complete inhibition at their high concentrations. Starch hydrolysis by amylase-antibody complexes was characterized by the growth of the formation glucose and triglycoside. These results are consistent with theoretical calculations of the antigenic determinant localization relative to components of the enzyme active center. Starch hydrolysis by amylase complexes with immobilized antibodies was also studied. A method of amylase detection with the help of the given system was developed. It was demonstrated that reactions between amylase-hormone (estradiol, testosterone) conjugates and hormone-specific antibodies also change the levels of the amylolytic activity.
Zherdev
A V
AV
Dzantiev
B V
BV
D000906:Antibodies; D001412:Bacillus subtilis / Q000201:enzymology*; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D013739:Testosterone / Q000378:metabolism; D000516:alpha-Amylases / Q000378:metabolism*
D000906:Antibodies; D013739:Testosterone; D004958:Estradiol; D000516:alpha-Amylases
Vliianie antitel na kataliticheskie svoĭstva batsilliarnoĭ alpha-amilazy.