Serological characterization and partial purification of an Lyt-1 homolog in tunicate hemocytes.
A panel of alloantisera and monoclonal antibodies specific to murine Lyt-1 allotypic and framework determinants was used in indirect immunofluorescence and FACS analysis to investigate the occurrence of an Lyt-1 homolog in tunicate (protochordate) hemocytes. Binding assays and quantitative absorption experiments established the expression of Lyt-1 cross-reacting determinants on a distinct population of tunicate hemocytes. These determinants were expressed exclusively by cells with the morphological characteristics of hemoblasts and lymphocytes. In a rapid two-step purification procedure, Lyt-1 glycoproteins from tunicate hemocytes and C57B1/6 mouse thymocytes were solubilized and partially purified by affinity chromatography using a mAb anti-Lyt-1 frame-work determinant. In both cell types, antigenic activities were associated with a major 67-kDa component. Our findings suggest an early phylogenetic emergence of an Lyt-1 homolog at this level of evolution.
Negm
H I
HI
Department of Anatomy and Cell Biology, School of Medicine, University of California, Los Angeles 90024-1763.
Mansour
M H
MH
Cooper
E L
EL
D000818:Animals; D000911:Antibodies, Monoclonal; D000950:Antigens, Ly / Q000032:analysis*; D003429:Cross Reactions; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006434:Hemocytes / Q000737:chemistry* / Q000166:cytology; D007962:Leukocytes / Q000737:chemistry / Q000166:cytology; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D014561:Urochordata / Q000737:chemistry* / Q000166:cytology
D000911:Antibodies, Monoclonal; D000950:Antigens, Ly