[Synthesis of sorbent from cross-linked starch for affinity purification of glucose(mannose)-specific lectins].
Cross-linked starch gel for the affinity chromatography of D-glucose (D-mannose)-specific lectins is suggested. In order to optimize hydrodynamic properties of gel 30% starch has been hydrolysed by HCI at 70 degrees C during 60 min and then cross-linked by epichlorohydrin under alkaline conditions. Every 100 g of starch require 18 ml of epichlorohydrin and 36 ml of 8 N KOH. The gel obtained has been successfully used for the purification of lectins from Pisum sativum L., Lens culinaris L., Vicia sativa L., and Vicia faba L. seeds. These lectins, purified on starch gel do not differ from sephadex-purified samples.
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D002846:Chromatography, Affinity; D005947:Glucose / Q000737:chemistry*; D007164:Immunosorbents / Q000138:chemical synthesis*; D037102:Lectins / Q000302:isolation & purification*; D008358:Mannose / Q000737:chemistry*; D013213:Starch / Q000737:chemistry*
D007164:Immunosorbents; D037102:Lectins; D013213:Starch; D005947:Glucose; D008358:Mannose
Syntez sorbentu na osnovi poperechno-zshitoho krokhmaliu dlia afinnoho ochyshchennia gliukozo(mannozo) spetsyfichnykh lektyniv.