title: Determination of nucleoside triphosphates by use of combined reactions of hexokinase and glucose-6-phosphate dehydrogenase.
abstract: ATP is known to be easily determined fluorometrically after it is utilized to produce the corresponding amount of NADPH by combined reactions of hexokinase and glucose-6-phosphate dehydrogenase. We studied further whether nucleoside triphosphates other than ATP can be also determined in a similar manner if they were incubated for a longer period with an increased amount of hexokinase. It was shown that CTP, GTP, ITP, and UTP can be utilized to produce the corresponding amount of NADPH after an incubation of at least 60 min and that 0 to 50 nmols of these nucleotides were able to be determined fluorometrically.
authors.affiliation: School of Materials Science, Toyohashi University of Technology, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Simofuruya
authors.forename: J
authors.initials: J
authors.lastname: Suzuki
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis / Q000378:metabolism; D003570:Cytidine Triphosphate / Q000032:analysis / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000737:chemistry; D006160:Guanosine Triphosphate / Q000032:analysis / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D007293:Inosine Triphosphate / Q000032:analysis / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009711:Nucleotides / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014544:Uridine Triphosphate / Q000032:analysis / Q000378:metabolism
chemical_list: D009711:Nucleotides; D007293:Inosine Triphosphate; D009249:NADP; D003570:Cytidine Triphosphate; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D014544:Uridine Triphosphate