title: High-performance liquid chromatographic determination of morphine, morphine-3-glucuronide, morphine-6-glucuronide and codeine in biological samples using multi-wavelength forward optical detection.
abstract: An isocratic high-performance liquid chromatographic method has been developed for the determination of morphine, morphine-3-glucuronide, morphine-6-glucuronide and codeine in plasma, urine and cerebrospinal fluid. The use of an efficient solid-phase extraction procedure together with a forward optical scanning detector allows a detection limit of 500 pg/ml. The method was evaluated by examination of biological samples taken from newborn infants following the intravenous administration of morphine sulfate.
authors.affiliation: Department of Pharmacodynamics, University of Illinois, Chicago 60612.
authors.forename: G
authors.initials: G
authors.lastname: Chari
authors.forename: A
authors.initials: A
authors.lastname: Gulati
authors.forename: R
authors.initials: R
authors.lastname: Bhat
authors.forename: I R
authors.initials: IR
authors.lastname: Tebbett
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003061:Codeine / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000652:urine*; D006801:Humans; D007231:Infant, Newborn; D007275:Injections, Intravenous; D009020:Morphine / Q000008:administration & dosage / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000652:urine*; D009022:Morphine Derivatives / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000652:urine*; D015203:Reproducibility of Results
chemical_list: D009022:Morphine Derivatives; C035349:morphine-6-glucuronide; D009020:Morphine; C018108:morphine-3-glucuronide; D003061:Codeine