[Detection of autoantibodies against phenylalanyl-, tyrosyl-, and tryptophanyl-tRNA-synthetase and anti-idiotypic antibodies to it in serum from patients with autoimmune diseases].
Sera of patients bearing autoimmune diseases (rheumatoid arthritis and systemic lupus erythematosus) and sera of clinically healthy donors were examined by ELISA for the presence of autoantibodies against tryptophanyl-, tyrosyl- and phenylalanyl-tRNA synthetases. Pure bovine synthetases served as antigens. It was shown that in patients with both autoimmune diseases all three enzyme autoantibodies were revealed at serum dilution 1/1600-1/3200. Moreover, by means of monoclonal antibodies against the same enzymes used for immunoaffinity sorption, antiidiotypic antibodies of IgG type against autoantibodies were detected. A conclusion has been made that autoimmune diseases are characterized by autoimmune response for many aminoacyl-tRNA synthetases irrespectively of their quaternary structure, intracellular location etc both at the level of primary and secondary antibodies.
Vartanian
O A
OA
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology*; D001327:Autoimmune Diseases / Q000097:blood / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology; D010652:Phenylalanine-tRNA Ligase / Q000276:immunology*; D014369:Tryptophan-tRNA Ligase / Q000276:immunology*; D014448:Tyrosine-tRNA Ligase / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D001323:Autoantibodies; D014448:Tyrosine-tRNA Ligase; D014369:Tryptophan-tRNA Ligase; D010652:Phenylalanine-tRNA Ligase
Vyiavlenie v syvorotkakh bol'nykh autoimmunnymi zabolevaniiami autoantitel protiv fenilalanil-, tirozil- i triptofanil-tRNK-sintetaz i antiidiotipicheskikh antitel k nim.