Monoclonal-antibody-based enzyme-linked immunosorbent assay for HMG 2b nonhistone protein in chick liver.
Monoclonal antibodies were prepared against the high mobility group (HMG) protein 2b from chick liver chromatin and a monoclonal-antibody-based enzyme-linked immunosorbent assay (ELISA) was developed for chick HMG 2b. The sensitivity of the assay is about ten times that of the previously described radioimmunoassay and solid-phase enzyme immunoassay for HMG proteins. With the use of ELISA technique, the amount of HMG 2b (micrograms protein/mg DNA) in the livers of 1-day old and 70-day old chicks was found to be 2.56 +/- 0.4, and 1.20 +/- 0.2, respectively. The age-dependent change in the level of HMG proteins probably reflects changes in the functional state of chromatin during ageing.
Kweon
S H
SH
Department of Nutritional Chemistry, School of Medicine, University of Tokushima, Japan.
Oka
T
T
Ito
M
M
Morita
M
M
Natori
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002645:Chickens / Q000254:growth & development / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006609:High Mobility Group Proteins / Q000032:analysis*; D008099:Liver / Q000737:chemistry* / Q000254:growth & development; D008297:Male; D051379:Mice; D011817:Rabbits; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D006609:High Mobility Group Proteins