[Intracellular pH and the proliferation of BHK-21 cells].
Data are presented on the dynamics of intracellular pH (pHi) in the course of growth of BHK-21 cells in suspension and on solid substrate. Cell proliferation in suspension in the presence of bicarbonate occurs at a mean value of pHi 6.76 +/- 0.02, which is only by 0.06 higher than that for resting cells. Adhesion of cells to the substrate cause a short (12 to 24 h) increase in pHi to 7.0-7.2, then proliferation of spread cells continued at pHi 6.8 +/- 0.03. Thus, for proliferation of substrate-independent BHK-21 cells to occur, there is no need for an additional alkalization of the cytoplasm at the expense of cell adhesion to a solid substrate, so the cells grow at low pHi values and at weak alkalization provided by adding serum. Data are presented that the Cl- and HCO(3-)-transport into the cell as well as Na+/H+ exchange are involved in pHi regulation. The decrease in pHi and inhibition of cell proliferation were observed in the presence of amiloride in bicarbonate-containing medium.
Akatov
V S
VS
Grobova
M E
ME
Koshevoĭ
Iu V
IuV
D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D001692:Biological Transport / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured / Q000166:cytology / Q000502:physiology; D002712:Chlorides / Q000378:metabolism; D006224:Cricetinae; D003470:Culture Media; D005456:Fluorescent Dyes; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000166:cytology* / Q000502:physiology; D012964:Sodium / Q000378:metabolism
D001639:Bicarbonates; D002712:Chlorides; D003470:Culture Media; D005456:Fluorescent Dyes; D006859:Hydrogen; D012964:Sodium
Vnutrikletochnyĭ pH i proliferatsiia kletok BHK-21.