title: Reinnervation of murine muscle following fetal sciatic nerve transection.
abstract: A technique is reported that permits transection of the sciatic nerve of mouse fetuses without interfering with fetal viability. Sciaticotomy was performed on Swiss Webster mice at day 17 of gestation; the contralateral side served as control. Six weeks later the extensor digitorum longus (EDL) muscles on both sides were injected with horseradish peroxidase (HRP). Examination of the lumbar spinal cord revealed that while a substantial number of motor neurons in the region of the spinal cord giving rise to the sciatic nerve died, the EDL muscle did become reinnervated. The size of the EDL motor neuron pool on the denervated-reinnervated side was approximately 43% of that seen on the control side. While the control EDL motor neuron pool was located in lumbar segments L3-L5, the location of the pool to the denervated-reinnervated EDL was shifted cranially to L2-L4. Denervated-reinnervated EDL muscles were analyzed immunohistochemically to study the effect of fetal denervation on the neuronal cell adhesion molecule (N-CAM) expression. At 2 weeks postnatal, N-CAM immunoreactivity in control muscle was segregated to the motor end-plate region, while fetally denervated muscle continued to express N-CAM along the length of the sarcolemma. Thus fetally denervated muscle does not develop the same pattern of N-CAM expression as normal, innervated muscle. By 6 weeks of age, the denervated-reinnervated muscle showed the same level and distribution of N-CAM immunoreactivity as did age-matched control muscle, indicating that most, if not all, of its myofibers had been reinnervated.
authors.affiliation: Department of Neurobiology, Anatomy, and Cell Science, University of Pittsburgh School of Medicine, Pennsylvania 15261.
authors.forename: R L
authors.initials: RL
authors.lastname: Mallonga
authors.forename: M
authors.initials: M
authors.lastname: Ontell
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D015816:Cell Adhesion Molecules, Neuronal / Q000096:biosynthesis; D016923:Cell Death / Q000502:physiology; D005260:Female; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D051379:Mice; D009045:Motor Endplate / Q000648:ultrastructure; D009046:Motor Neurons / Q000502:physiology; D009121:Muscle Denervation; D009132:Muscles / Q000294:innervation*; D009416:Nerve Regeneration / Q000502:physiology; D011247:Pregnancy; D012584:Sciatic Nerve / Q000502:physiology*; D013116:Spinal Cord / Q000033:anatomy & histology
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D006735:Horseradish Peroxidase