Genetic and biochemical characterization of a new chymotrypsin isozyme of the house mouse, CTRA-1.
Genetic variation of a codominantly inherited pancreas protease, designated CTRA-1, was discovered in the house mouse by isoelectric focusing in polyacrylamide gels. Phenotype CTRA-1A was found in MOLH/Fre and in the majority of common laboratory mouse strains. Phenotype CTRA-1B was found in PWD/Ph. It was characterized by the absence of a corresponding protease band. A third phenotype, CTRA-1C, was observed in IS/Cam and a fourth phenotype, CTRA-1D, was detected in SEG/1. CTRA-1 was found only in the pancreas and may represent the A form of chymotrypsin. The enzyme was shown to be controlled by the presumed structural locus Ctra-1 located on chromosome 8. From two backcross series, including a total of 274 animals, the gene order (Es-1, Es-9)-3.9 +/- 1.7%-Got-2-3.9 +/- 1.7%-(Es-2, Es-7, Es-23)-0.7 +/- 0.5%- Ctra-1-6.3 +/- 2.2%-Prt-2 was established.
von Deimling
O H
OH
Abteilung für Chemische Pathologie, Universität Freiburg, Bundesrepublik Deutschland.
Gröning
J
J
Gaa
A
A
D000818:Animals; D002874:Chromosome Mapping; D002918:Chymotrypsin / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D003433:Crosses, Genetic; D005260:Female; D005796:Genes; D005819:Genetic Markers; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008297:Male; D051379:Mice / Q000235:genetics* / Q000378:metabolism; D008815:Mice, Inbred Strains / Q000235:genetics / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology; D010641:Phenotype; D013045:Species Specificity; D013379:Substrate Specificity
D005819:Genetic Markers; D007527:Isoenzymes; D002918:Chymotrypsin