title: Daflon as a cellular antioxidant and a membrane-stabilizing agent in human fibroblasts irradiated by ultraviolet A radiation.
abstract: Daflon is a strong inhibitor of Cu(2+)-induced arachidonic acid peroxidation, as revealed by the inhibition of thiobarbituric acid-reactive substance formation in mixed liposomes of phosphatidylcholine and arachidonic acid. Diosmin, the major Daflon constituent, is a good complexant of Cu2+ ions but not of Fe2+ ions. The Cu2+ complex formation may thus explain part of the antioxidant effect. However, Daflon is also a good quencher of the singlet oxygen-induced arachidonic acid peroxidation that does not involve metal ions. When cultured human fibroblasts are incubated with the diosmin-hesperidin mixture constituting Daflon, the latter is as efficient as vitamin E in inhibiting the ultraviolet A-induced lipid peroxidation and plasma membrane damage measured by lactate dehydrogenase release.
authors.affiliation: Muséum National d'Histoire Naturelle, Laboratoire de Physico-Chimie de l'Adaptation Biologique, Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Santus
authors.forename: L
authors.initials: L
authors.lastname: Perdrix
authors.forename: J
authors.initials: J
authors.lastname: Haigle
authors.forename: P
authors.initials: P
authors.lastname: Morliere
authors.forename: J C
authors.initials: JC
authors.lastname: Maziere
authors.forename: C
authors.initials: C
authors.lastname: Maziere
authors.forename: C
authors.initials: C
authors.lastname: Labrid
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D001095:Arachidonic Acids / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured; D003300:Copper / Q000378:metabolism; D004145:Diosmin / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D006569:Hesperidin / Q000494:pharmacology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation; D014466:Ultraviolet Rays / Q000009:adverse effects; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D001095:Arachidonic Acids; D014810:Vitamin E; D003300:Copper; D004145:Diosmin; D006569:Hesperidin; D007770:L-Lactate Dehydrogenase