Immunological studies with the venom of the scorpion Tityus serrulatus.
1. We describe a "sandwich" enzyme-linked immunosorbent assay (ELISA) sensitive to quantities of scorpion (Tityus serrulatus) venom (TsV) in the range of 1-3 ng/ml sample. 2. Cross-reactivity with the venom from the rattlesnake Crotalus durissus terrificus and with venoms from several snakes of the Bothrops genus was detected only at concentrations higher than 1 microgram/ml sample. 3. A conventional ELISA is also described for the detection of antibodies against TsV. 4. Analysis by Western Blot (WB) demonstrated a 25-kDa protein band common to TsV and to the venoms of Bothrops moojeni, B. jararacussu and B. jararaca. 5. Venom from C. d. terrificus exhibited WB cross-reactive bands of 16 and 25 kDa with TsV.
Barral-Netto
M
M
Serviço de Imunologia, Hospital Universitário Prof. Edgard Santos, Universidade Federal da Bahia, BA, Brasil.
Vinhas
V
V
Schriefer
A
A
Barral
A
A
Santos
S B
SB
Almeida
A R
AR
Novaes
G
G
D000818:Animals; D015153:Blotting, Western; D002846:Chromatography, Affinity; D003429:Cross Reactions; D003435:Crotalid Venoms / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005240:Feasibility Studies; D008297:Male; D011817:Rabbits; D012604:Scorpion Venoms / Q000032:analysis* / Q000276:immunology
D003435:Crotalid Venoms; D012604:Scorpion Venoms