title: [Clinical application of Tc-99m HMPAO labeled leukocyte imaging in inflammatory disease].
abstract: A radionuclide imaging with Tc-99m HMPAO labeled leukocyte was performed in order to determine its clinical usefulness in inflammatory disease. The mixed leukocyte isolated from 40 ml of whole blood containing 5 ml of acid citrate dextrose A and 7 ml of 6% hydroxyethyl starch was incubated with 370 MBq (10 mCi) of Tc-99m HMPAO at 37 degrees C for 30 minutes. Because the labeling efficiency of Tc-99m HMPAO labeled leukocyte was 60.2 +/- 6.3%, the procedure of washing Tc-99m, leukocyte with 5 ml of physiological saline was necessary before intravenous injection, in order to remove the unlabeled Tc-99m HMPAO. The recoveries of Tc-99m leukocyte in the blood after intravenous injection were 41.1 +/- 6.7% at 5 minutes, 33.4 +/- 2.1% at 30 minutes, and 27.2 +/- 3.4% at 2 hours after injection. Moreover, the labeled leukocyte was not stained with trypan blue. Therefore, the biological activity of the Tc-99m leukocyte was maintained as that of In-111 oxine labeled leukocyte. In the 39 patients with clinical suspicion of inflammatory disease including 15 patients with acute and chronic infectious disease where both Tc-99m leukocyte and Ga-67 citrate imagings were performed, the sensitivity, specificity and accuracy for infectious disease were 47%, 100% and 79% with Tc-99m leukocyte, and 67%, 79% and 74% with Ga-67 citrate. These results suggest that Tc-99m HMPAO labeled leukocyte imaging is promising for evaluating inflammatory disease because of the much higher specificity, the ready availability of Tc-99m HMPAO, the good image quality, and the lower radiation dose to the patient.
authors.affiliation: Department of Nuclear Medicine, Kanazawa University Hospital.
authors.forename: T
authors.initials: T
authors.lastname: Aburano
authors.forename: J
authors.initials: J
authors.lastname: Taki
authors.forename: S
authors.initials: S
authors.lastname: Kinuya
authors.forename: N
authors.initials: N
authors.lastname: Shuke
authors.forename: K
authors.initials: K
authors.lastname: Yokoyama
authors.forename: T
authors.initials: T
authors.lastname: Takayama
authors.forename: N
authors.initials: N
authors.lastname: Tonami
authors.forename: K
authors.initials: K
authors.lastname: Hisada
authors.forename: H
authors.initials: H
authors.lastname: Terada
mesh_terms: D005710:Gallium Radioisotopes; D006801:Humans; D007249:Inflammation / Q000000981:diagnostic imaging*; D007962:Leukocytes; D015609:Organotechnetium Compounds; D010091:Oximes; D011237:Predictive Value of Tests; D011877:Radionuclide Imaging; D012680:Sensitivity and Specificity; D019690:Technetium Tc 99m Exametazime
chemical_list: D005710:Gallium Radioisotopes; D015609:Organotechnetium Compounds; D010091:Oximes; D019690:Technetium Tc 99m Exametazime