[Use of the principle of reverse problems for detecting processes, occurring in cells suspended in culture. II. Distribution of glutathione, SH groups, and optical density in the E. coli cell cycle].
A method was proposed for calculating the content of intracellular components during the cell cycle of an individual cell. The principle of reverse problems was used in the mathematical model proposed. The model allowed us to calculate changes of intracellular parameters of an individual cell from corresponding parameters measured in the whole culture. Optical density, total SH-group and glutathione content in synchronous culture of E. coli were the parameters studied. The proposed method may be applied for both synchronous and asynchronous cell cultures.
Kazarian
L S
LS
Fishov
I L
IL
Rybina
V V
VV
Evtodienko
Iu V
IuV
D002453:Cell Cycle; D004926:Escherichia coli / Q000166:cytology* / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D008962:Models, Theoretical; D055095:Optics and Photonics; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D013438:Sulfhydryl Compounds; D005978:Glutathione
Primenenie printsipa obratnykh zadach dliia vyiavleniia protsessov, proiskhodiashchikh v kletkakh pri ikh suspenzionnom kul'tirovanii. II. Opredelenie glutationa, SH-grupp i opticheskoĭ plotnosti v kletochnom tsikle E. coli.