Progastrin and its products in the cerebellum.
So far the only CNS neurons found to express gastrin have been hypothalamohypophyseal. Using a library of radioimmunoassays specific for different sequences of porcine progastrin in combination with chromatography and enzyme cleavages, 21 or 24 porcine cerebelli was now found to contain progastrin or its products. Sixteen cerebelli processed progastrin via glycine-extended intermediates to bioactive, carboxyamidated gastrin-17 and -34, half of the gastrins being tyrosine O-sulfated. The mean concentration of carboxyamidated gastrins was 0.8 pmol/g tissue (range less than 0.1-2.1 pmol/g), of glycine-extended intermediates 0.1 pmol/g (range less than 0.1-0.3 pmol/g) and of progastrin 0.4 pmol/g (range less than 0.1-1.0 pmol/g). The results show that the gastrin gene is expressed in more than a single region of the brain.
Rehfeld
J F
JF
University Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark.
D000595:Amino Acid Sequence; D000818:Animals; D002531:Cerebellum / Q000737:chemistry*; D002850:Chromatography, Gel; D005755:Gastrins / Q000737:chemistry / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D011487:Protein Conformation; D011498:Protein Precursors / Q000737:chemistry / Q000302:isolation & purification*; D011863:Radioimmunoassay; D013552:Swine
D005755:Gastrins; D010446:Peptide Fragments; D011498:Protein Precursors; C013706:big gastrin