Exploitation of a 650 bp probe for quantification of Pneumocystis carinii.
Signals obtained from serial dilutions of rat-derived Pneumocystis carinii DNA were used to assess the sensitivity of a 650-bp DNA probe which recognises both cystic and non-cystic forms. Enhanced chemiluminescence was selected as a non-radioactive detection method and signals could be semi-quantitated by scanning densitometry. This technique was used to examine the inhibitory effect of pentamidine in vitro indicating that DNA probes might be useful tools in the search for a novel therapeutic agent.
Hancock
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Chemotherapy Department, Glaxo Group Research Ltd., Greenford, Middx, UK.
Christodoulou
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C
Jackson
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HC
D000818:Animals; D015342:DNA Probes; D004271:DNA, Fungal / Q000302:isolation & purification*; D003720:Densitometry; D008168:Lung / Q000382:microbiology; D011010:Pneumocystis / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains
D015342:DNA Probes; D004271:DNA, Fungal