Characterization of an Agrobacterium tumefaciens lectin.
An Agrobacterium tumefaciens suspension induces a strong agglutination of aldehyde-fixed pig erythrocytes at pH 5.0. The agglutination is inhibited by some polysaccharides, such as fucoidin, and also when the pH is raised to 7.0. Lectins (sugar-binding proteins) associated with the bacterial cell wall of A. tumefaciens strain 84.5 were directly evidenced by spectrofluorimetry using fluoresceinylated neoglycoproteins. The specific binding of the fluorescein-labelled neoglycoprotein bearing alpha-L-fucoside residues was also optimal at pH 5.0. A lectin was purified by affinity chromatography on agarose substituted with alpha-L-fucopyranoside. Furthermore, the haemagglutination activity of this lectin was inhibited by polysaccharides isolated from poplar leaves.
Depierreux
C
C
Département de Biochimie des Glycoconjugués et Lectines Endogènes, Centre de Biophysique Moléculaire, C.N.R.S., Orléans, France.
Kang
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HC
Guérin
B
B
Monsigny
M
M
Delmotte
F
F
D016960:Agrobacterium tumefaciens / Q000276:immunology*; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000378:metabolism; D006384:Hemagglutination / Q000187:drug effects; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007525:Isoelectric Focusing; D037102:Lectins / Q000302:isolation & purification*; D008970:Molecular Weight; D010936:Plant Extracts; D037121:Plant Lectins; D011134:Polysaccharides / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013552:Swine; D014197:Trees
D002241:Carbohydrates; D006023:Glycoproteins; D037102:Lectins; D010936:Plant Extracts; D037121:Plant Lectins; D011134:Polysaccharides