title: High-performance liquid chromatography of thiazolidinic compounds obtained by condensation of pyridoxal 5'-phosphate or pyridoxal with aminothiols (L- or D-cysteine, cysteamine, L-cysteine ethyl ester).
abstract: We investigated six thiazolidine 4-carboxylic acids of biological interest, obtained by condensation of pyridoxal 5'-phosphate or pyridoxal with L- or D-cysteine, cysteamine or L-cysteine ethyl ester. A reversed-phase high-performance liquid chromatographic method, using a C18 column for their separation, was developed by sequential optimization of the pH and the gradient of the mobile phase. Resolution of the compounds was obtained with an analysis time of less than 20 min.
authors.affiliation: Institute of Biochemistry and Enzymology, University of Siena, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Terzuoli
authors.forename: R
authors.initials: R
authors.lastname: Leoncini
authors.forename: D
authors.initials: D
authors.lastname: Vannoni
authors.forename: E
authors.initials: E
authors.lastname: Marinello
authors.forename: R
authors.initials: R
authors.lastname: Pagani
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003543:Cysteamine / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry*; D008099:Liver; D011730:Pyridoxal / Q000737:chemistry*; D011732:Pyridoxal Phosphate / Q000737:chemistry*; D013844:Thiazoles / Q000032:analysis* / Q000138:chemical synthesis; D014020:Tissue Extracts
chemical_list: D013844:Thiazoles; D014020:Tissue Extracts; D011730:Pyridoxal; D003543:Cysteamine; D011732:Pyridoxal Phosphate; D003545:Cysteine