title: Development of Neisseria gonorrhoeae in vitro susceptibility test methods for cefixime including quality control guidelines.
abstract: Cefixime was tested in a multilaboratory study to establish susceptibility testing interpretive criteria and quality control (QC) guidelines for Neisseria gonorrhoeae. Interpretive criteria were established by using triplicate testing of 102 representative gonococcal strains. Only a susceptible category was proposed for cefixime (greater than or equal to 31 mm and less than or equal to 0.25 micrograms/ml) because of very rare, resistant isolates. QC ranges [N. gonorrhoeae American Type Culture Collection (ATCC) 49226] were established using multiple GC agar lots, three disk lots, and a number of test replicates consistent with the National Committee for Clinical Laboratory Standards (NCCLS) M23-T guidelines. Cefixime was stable in GC agar medium for at least 21 days when stored at 2 degrees-5 degrees C. Oral third-generation cephalosporins, such as cefixime, appear to represent potentially useful clinical alternatives to parenteral drugs of the same class for single-dose therapy of gonorrhea.
authors.affiliation: University of Iowa Hospitals and Clinics, College of Medicine, Iowa City.
authors.forename: R N
authors.initials: RN
authors.lastname: Jones
authors.forename: E H
authors.initials: EH
authors.lastname: Gerlach
authors.forename: F P
authors.initials: FP
authors.lastname: Koontz
authors.forename: P R
authors.initials: PR
authors.lastname: Murray
authors.forename: M A
authors.initials: MA
authors.lastname: Pfaller
authors.forename: J A
authors.initials: JA
authors.lastname: Washington
authors.forename: M E
authors.initials: ME
authors.lastname: Erwin
authors.forename: C C
authors.initials: CC
authors.lastname: Knapp
mesh_terms: D020682:Cefixime; D002439:Cefotaxime / Q000031:analogs & derivatives* / Q000494:pharmacology; D002443:Ceftriaxone / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004355:Drug Stability; D008826:Microbial Sensitivity Tests / Q000592:standards*; D009344:Neisseria gonorrhoeae / Q000187:drug effects*; D011786:Quality Control; D012044:Regression Analysis
chemical_list: D002443:Ceftriaxone; D020682:Cefixime; D002439:Cefotaxime