title: Synergistic effect of two cell recognition systems: glycosphingolipid-glycosphingolipid interaction and integrin receptor interaction with pericellular matrix protein.
abstract: GM3-expressing cells adhere, spread and migrate on plastic plates coated with Gg3, LacCer and Gb4, but not with other glycosphingolipids (GSLs). Thus, cell adhesion, spreading and migration through GSL-GSL interaction occur in an analogous fashion to the interaction of cells with adhesive matrix proteins [AP, e.g. fibronectin (FN), laminin (LN)] through their integrin receptors. In this study, the adhesion of two GM3-expressing cell lines (B16 melanoma and HEL299 fibroblast) on plastic plates co-coated with GSL plus AP is compared with adhesion on plates coated with GSL (Gg3 or LacCer) alone, or coated with AP alone. Results show that: (i) cell adhesion on GSL-coated plates takes place earlier in the incubation period than that on AP-coated plates; (ii) cell adhesion, as well as spreading, was greatly enhanced (in terms of strength and rapidity) on plates co-coated with GSL plus AP; (iii) repulsion (negative adhesion) of cells was observed on plates co-coated with AP plus N-acetyl-GM3 (NAcGM3) and was presumably based on repulsive NAcGM3-NAcGM3 interaction; (iv) GM3-dependent cell adhesion on GSL-coated plates, as well as synergistic promotion of cell adhesion (based on the GSL-GSL and AP-integrin systems), was suppressed by incubation of cells with anti-GM3 monoclonal antibody DH2 or sialidase. Synergistic adhesion of cells on GSL/AP co-coated plates was less inhibited by incubation with peptide sequences RGDS or YIGSR than was adhesion on plates coated with AP alone.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Biomembrane Institute, Seattle, WA 98119.
authors.forename: N
authors.initials: N
authors.lastname: Kojima
authors.forename: S
authors.initials: S
authors.lastname: Hakomori
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D002448:Cell Adhesion / Q000187:drug effects*; D002462:Cell Membrane / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects; D005353:Fibronectins / Q000494:pharmacology*; D006028:Glycosphingolipids / Q000378:metabolism* / Q000494:pharmacology; D016023:Integrins / Q000378:metabolism*; D007700:Kinetics; D007797:Laminin / Q000494:pharmacology*; D008546:Melanoma, Experimental; D051379:Mice; D008954:Models, Biological; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000494:pharmacology; D009842:Oligopeptides / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D005353:Fibronectins; D006028:Glycosphingolipids; D016023:Integrins; D007797:Laminin; D009842:Oligopeptides; D009439:Neuraminidase