Cholesterol exchange between human serum isolated lipoproteins. Effect of protein concentration.
The in vitro transfer of radiolabelled unesterified cholesterol from human low- and very low- to high-density lipoproteins in the presence of either lipoprotein deficient serum or bovine serum albumin has been studied. The rate of transfer was faster from LDL (t1/2 = 44 min) than from VLDL (55 min). The presence of 2% protein had no effect on the transfer. However, 10% albumin or lipoprotein deficient serum reduced t1/2 by 35%, which indicates no specific effect of a plasma protein on the rate of transfer of unesterified cholesterol.
Velazquez
E
E
Departamento de Bioquímica, Facultad de Medicina, Universidad Complutense, Madrid, Spain.
Montes
A
A
Ruiz-Albusac
J M
JM
D001692:Biological Transport / Q000187:drug effects; D002784:Cholesterol / Q000097:blood*; D006801:Humans; D007700:Kinetics; D008074:Lipoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D008075:Lipoproteins, HDL / Q000302:isolation & purification / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000302:isolation & purification / Q000494:pharmacology; D008079:Lipoproteins, VLDL / Q000302:isolation & purification / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000494:pharmacology*
D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D012710:Serum Albumin, Bovine; D002784:Cholesterol