[The properties of monoclonal antibodies to HIV gag gene proteins].
Immunization of BALB/c mice with natural purified HIV antigen, fusion of spleen cells with myeloma cells and subsequent selection of hybrid clones using recombinant gag antigen of HIV gave hybridomas producing monoclonal antibodies (MCA) to HIV. The immune blotting method demonstrated that 3 clones interacted with protein p24 and 4 clones with protein p17 of HIV. Competitive EIA led to a conclusion that the resulting MCA detected at least 3 antigenic determinants in proteins, products of gag gene of HIV. The potentials of using these MCA for the detection of viral antigen in HIV-infected continuous cell lines were demonstrated.
Kushch
A A
AA
Tugizov
Sh M
ShM
Simonov
V I
VI
Timofeev
I V
IV
Perminova
N P
NP
Shumaĭ
E P
EP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity / Q000276:immunology; D015683:Gene Products, gag / Q000276:immunology*; D015488:HIV Antigens / Q000032:analysis; D015497:HIV-1 / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007124:Immunoenzyme Techniques; D051379:Mice; D011994:Recombinant Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D015683:Gene Products, gag; D015488:HIV Antigens; D011994:Recombinant Proteins
Svoĭstva monoklonal'nykh antitel k belkam gena gag VICh.