Biodegradation and antitumour effect of adriamycin-containing poly(L-lactic acid) microspheres.
Adriamycin-containing poly (L-lactic acid) microspheres were prepared to develop a slow-releasing and long-acting adriamycin delivery system. An almost constant release of adriamycin from the adriamycin-containing poly(L-lactic acid) was achieved in Tris buffer and adriamycin disappeared within 20 d. Adriamycin was not detected in serum for up to 14 d, when the suspension of the adriamycin-containing poly(L-lactic acid) microspheres was injected into lung parenchyma, the femoral muscles of rabbits or the peritoneal cavity of mice. However, adriamycin remained in the rabbit muscles for up to 10 d under formation of scar tissue. When free adriamycin was added to P815 tumour cells in culture, the cell survival rate decreased with the exposure time. The treatment with the adriamycin-containing poly(L-lactic acid) microspheres showed a higher survival rate for mice bearing P815 tumour cells than with free adriamycin. In addition, the systemic side effects were insignificant when the adriamycin-containing poly(L-lactic acid) microspheres were given to mice instead of free adriamycin.
Ike
O
O
Research Centre for Biomedical Engineering, Kyoto University, Japan.
Shimizu
Y
Y
Ikada
Y
Y
Watanabe
S
S
Natsume
T
T
Wada
R
R
Hyon
S H
SH
Hitomi
S
S
D000818:Animals; D001673:Biodegradation, Environmental; D003692:Delayed-Action Preparations; D004058:Diffusion; D004317:Doxorubicin / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism* / Q000627:therapeutic use*; D066298:In Vitro Techniques; D007773:Lactates / Q000737:chemistry*; D019344:Lactic Acid; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008863:Microspheres; D009132:Muscles / Q000378:metabolism / Q000473:pathology; D010529:Peritoneal Cavity; D010534:Peritoneal Neoplasms / Q000188:drug therapy; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011817:Rabbits; D013499:Surface Properties; D014407:Tumor Cells, Cultured / Q000187:drug effects
D003692:Delayed-Action Preparations; D007773:Lactates; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D004317:Doxorubicin