title: Relation between hyaluronan and sulphated glycosaminoglycan synthesis and degradation in cultured embryonic fibroblasts. Effect of concanavalin A and ammonium chloride administration.
abstract: In order to evaluate the relationship between glycosaminoglycan (GAG) synthesis and degradation, the effect of NH4Cl, which inhibits lysosomal degradation, on GAG production was analysed in vitro in concanavalin A (Con A) stimulated fibroblasts from 7 and 14-day-old chick embryos. 35SO4 incorporation into total proteoglycan (PG), 3H incorporation into individual GAG classes, beta-N-acetyl-D-glucosaminidase and beta-D-glucuronidase activity were determined. The results indicate a correlation between Con A and NH4Cl effects: NH4Cl induced a reduction principally in the GAG classes most stimulated by Con A. Thus HA and DS are much more stimulated by Con A and inhibited by NH4Cl than are CS and HS.
authors.affiliation: Institute of Histology and General Embryology, University of Ferrara, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Carinci
authors.forename: P
authors.initials: P
authors.lastname: Locci
authors.forename: R
authors.initials: R
authors.lastname: Evangelisti
authors.forename: L
authors.initials: L
authors.lastname: Marinucci
authors.forename: L
authors.initials: L
authors.lastname: Rossi
authors.forename: E
authors.initials: E
authors.lastname: Becchetti
mesh_terms: D000118:Acetylglucosaminidase / Q000032:analysis; D000643:Ammonium Chloride / Q000494:pharmacology*; D000818:Animals; D002642:Chick Embryo; D002852:Chromatography, Ion Exchange; D003208:Concanavalin A / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D005944:Glucosamine / Q000378:metabolism; D005966:Glucuronidase / Q000032:analysis; D006025:Glycosaminoglycans / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000378:metabolism*; D012867:Skin / Q000166:cytology; D013431:Sulfates / Q000378:metabolism
chemical_list: D006025:Glycosaminoglycans; D013431:Sulfates; D000643:Ammonium Chloride; D003208:Concanavalin A; C013786:A73025; D006820:Hyaluronic Acid; D005966:Glucuronidase; D000118:Acetylglucosaminidase; D005944:Glucosamine