Determination of hippuric acid and o-, m- and p-methylhippuric acids in urine by capillary gas chromatography.
A capillary gas chromatographic (GC) method for the simultaneous determination of urinary hippuric acid (HA) and o-, m- and p-methylhippuric acids (MHAs), metabolites of toluene and o-, m- and p-xylenes, respectively, is described. These metabolites are converted into their isopropyl derivatives by extractive alkylation with tetrahexylammonium ion as extracting agent and isopropyl bromide as alkylating reagent in benzene. The derivatives are analysed using a chromatograph equipped with hydrogen flame ionization detector, split injection system and DB-17 capillary column. Benzoylleucine is used as an internal standard. The derivatives are well separated within 5 min and no interfering peaks are observed. The calibration curves of HA and MHAs in the range 1-50 micrograms are linear and sufficiently reproducible for quantitative analysis. Urine can be analysed accurately and precisely by this method without prior clean-up of the sample.
Kataoka
H
H
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Manabe
K
K
Nakase
S
S
Makita
M
M
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D006626:Hippurates / Q000652:urine*; D006801:Humans; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D006626:Hippurates