Derivative spectrophotometric assay of acetaminophen and spectrofluorimetric determination of its main impurity.
Second derivative UV spectrophotometry proved to be a useful tool for the determination of acetaminophen, even in the presence of high amounts of the impurity, 4-aminophenol and the background effect caused by dissolved excipients of some dosage forms. The fluorimetric method used for the estimation of 4-aminophenol impurity is less sensitive than the colorimetric methods used in the pharmacopeias. This can be attributed to an inner-filter effect.
Milch
G
G
CHINOIN Pharmaceutical and Chemical Works Ltd, Quality Control Department, Budapest, Hungary.
Szabó
E
E
D000082:Acetaminophen / Q000032:analysis*; D000627:Aminophenols / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D000627:Aminophenols; D000082:Acetaminophen; C026729:4-aminophenol