Transfructosylation of rebaudioside A (a sweet glycoside of Stevia leaves) with Microbacterium beta-fructofuranosidase.
It was found that a beta-fructofuranosidase produced by Microbacterium sp. H-1 has potent trans-beta-fructofuranosylation activity from sucrose (donor). By means of this enzyme system, rebaudioside A (RA), the second major sweet steviol glycoside of the leaves of Stevia rebaudiana, was subjected to transfructosylation, affording a mono-beta-fructofuranosylated product (RA-F) in a high yield. The structure of RA-F was elucidated as beta-D-fructofuranosyl-(2----6)-beta-D-glucopyranosyl ester of steviol-13-O-[beta-D-glucopyranosyl-(1----2)]- [beta-D-glucopyranosyl-(1----3)]-beta-D-glucopyranoside. Some improvement in the quality of sweetness was observed for RA-F.
Ishikawa
H
H
Hokkaido Sugar Co., Ltd., Tokyo, Japan.
Kitahata
S
S
Ohtani
K
K
Tanaka
O
O
D000192:Actinomycetales / Q000201:enzymology*; D045786:Diterpenes, Kaurane; D005632:Fructose / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D010944:Plants / Q000737:chemistry*; D013549:Sweetening Agents / Q000737:chemistry*; D013729:Terpenes / Q000737:chemistry*; D043324:beta-Fructofuranosidase
D045786:Diterpenes, Kaurane; D013549:Sweetening Agents; D013729:Terpenes; D005632:Fructose; C025474:rebaudioside A; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase