title: Enantiospecific analysis of ketoprofen in plasma by high-performance liquid chromatography.
abstract: A high-performance liquid chromatographic (HPLC) assay for the determination of the R- and S-enantiomers of ketoprofen is described. Facile ketoprofen extraction from plasma and derivatization to the diastereomeric S-1-phenylethylamides was followed by normal-phase HPLC. The ketoprofen diastereomeric amides eluted within 8 min. The limit of quantification of the assay was 0.15 mg/l of each enantiomer (signal-to-noise ratio = 5).
authors.affiliation: Pharmacy Department, Repatriation General Hospital, Daw Park, SA, Australia.
authors.forename: P J
authors.initials: PJ
authors.lastname: Hayball
authors.forename: R L
authors.initials: RL
authors.lastname: Nation
authors.forename: F
authors.initials: F
authors.lastname: Bochner
authors.forename: R K
authors.initials: RK
authors.lastname: Le Leu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007660:Ketoprofen / Q000097:blood*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: D007660:Ketoprofen