Involvement of the cytoskeleton components in the secretion of luteinization stimulator by porcine granulosa cells.
The involvement of cytoskeletal proteins in the gonadotropic regulation of granulosa cell progesterone (P4) and luteinization stimulator (LS) secretion was studied. The effect of cytochalasin B (CB) and colchicine (Col) on LS secretion was assessed in 4-day culture of porcine granulosa cells isolated from medium and large size follicles (MGC+LGC). The amount of LS released into the culture medium (CM) was determined by adding this LS-enriched CM to the cultures of granulosa cells isolated from small follicles (SGC) and by measuring P4 produced by them. Col (0.1 and 100 mumol l-1 and CB 2.0 mumol l-1) inhibited P4 synthesis by SGC. The secretion of LS was significantly reduced by Col at both concentrations used, but not by CB. These findings indicate specific involvement of microtubules in the secretion of luteinization stimulator by granulosa cells.
Danisová
A
A
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava.
Kolena
J
J
D008841:Actin Cytoskeleton / Q000187:drug effects / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D007986:Luteinizing Hormone / Q000032:analysis / Q000378:metabolism*; D011374:Progesterone / Q000032:analysis / Q000378:metabolism; D011863:Radioimmunoassay; D013552:Swine
D003470:Culture Media; D003571:Cytochalasin B; D011374:Progesterone; D007986:Luteinizing Hormone; D003078:Colchicine