Molecular structure of Tcp-10 genes from the t complex responder locus.
Male transmission ratio distortion (TRD) is a property of mouse t haplotypes which requires the t complex responder locus (Tcr). Tcr has been localized to a 70-160 kb region in t haplotypes. A candidate gene for the responder, called Tcp-10bt, has been cloned and is one member of a highly related gene family called Tcp-10 (formerly T66). Molecular evidence suggests that unique alternative splicing of the Tcp-10bt gene may be responsible for the mutant responder activity. Here we present the intron/exon structure of a representative Tcp-10 gene, and the characterization of alternative polyadenylation sites. The Tcp-10 genes contain 12 exons which span approximately 21 kb of DNA. At least six different polyadenylation sites are used, and none have a perfect consensus signal. This appears to be a common feature associated with testes-expressed transcripts. Since the gene we have analyzed is absent from many t haplotypes without apparent consequence, and no corresponding cDNAs have been isolated, it was speculated to be a pseudogene. However, no major sequence differences were found within the coding sequence to conclude that Tcp-10pst is a pseudogene.
Bullard
D C
DC
Department of Genetics, Case Western Reserve University, Cleveland, Ohio 44106.
Schimenti
J C
JC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D005091:Exons; D047908:Intracellular Signaling Peptides and Proteins; D007438:Introns; D008297:Male; D051379:Mice; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D009687:Nuclear Proteins / Q000235:genetics*; D011061:Poly A / Q000378:metabolism; D011506:Proteins; D011544:Pseudogenes; D015183:Restriction Mapping; D016415:Sequence Alignment; D014158:Transcription, Genetic; D044767:Ubiquitin-Protein Ligases; D056427:t-Complex Genome Region
D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D011506:Proteins; C533392:TCP10L3 protein, human; C533395:Tcp10 protein, mouse; D011061:Poly A; D004247:DNA; C116196:PPP1R11 protein, human; D044767:Ubiquitin-Protein Ligases