[Transduction of a marker gene (Neor) into precursor cells of the hematopoietic microenvironment].
The attempt of retroviral transfer of the bacterial Neor gene into stromal precursor cells able to transfer haemopoietic microenvironment and to long-term support of haemopoiesis in vitro and in vivo was made. The existence of marker gene in stromal cells was established by the method of polymerase chain reaction. The transduced stromal precursor cells create normal haemopoietic microenvironment. The data obtained would be important for the further investigation of proliferation and differentiation of stromal precursor cells.
Drize
N I
NI
Surin
V L
VL
Gan
O I
OI
Deriugina
E I
EI
Chertkov
I L
IL
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis / Q000235:genetics; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000648:ultrastructure*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012190:Retroviridae / Q000235:genetics; D014161:Transduction, Genetic / Q000235:genetics*
D005819:Genetic Markers; D004247:DNA
Transduktsiia markernogo gena (Neor) v kletki-predshestvenniki krovetvornogo mikrookruzheniia.