DNA footprint enhancement using tandem binding sites.
A concatenated DNA fragment containing a five-repeat binding site was used for DNase I footprinting. Under the same conditions, the tandem repeat assay greatly enhanced the DNA footprint as compared with a native DNA sequence with only one binding site. This technique provides an approach for improving poor DNA footprints.
Zhang
W W
WW
Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030.
Farrés
J
J
Busch
H
H
D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012359:tRNA Methyltransferases
D009687:Nuclear Proteins; D004247:DNA; C065028:NOP2 protein, human; D012359:tRNA Methyltransferases; D003850:Deoxyribonuclease I