title: Structure of equine corticotropin releasing factor.
abstract: A 41 amino acid peptide, probably identical in structure to human corticotropin releasing factor, was isolated from 70 equine hypothalami by methanol extraction, immunoaffinity chromatography and single step of reverse phase HPLC. The amino acid sequence was determined by gas phase sequence analysis. Probable carboxyl terminal amidation was demonstrated by similar retention times for equine and human corticotropin releasing factor on reverse phase HPLC at pH 8. The likely structure of equine corticotropin releasing factor is: Ser-Glu-Glu-Pro-Pro- Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Ala-Arg- Ala-Glu - Gln-Leu-Ala-Gln-Gln-Ala-His-Ser-Asn-Arg-Lys-Leu-Met-Glu-Ile-Ile-NH2. The purified peptide is equipotent with human corticotropin releasing factor in an in vitro bioassay and in a human plasma binding protein assay.
authors.affiliation: Department of Endocrinology, Princess Margaret Hospital, Christchurch, New Zealand.
authors.forename: J H
authors.initials: JH
authors.lastname: Livesey
authors.forename: A
authors.initials: A
authors.lastname: Carne
authors.forename: C H
authors.initials: CH
authors.lastname: Irvine
authors.forename: J
authors.initials: J
authors.lastname: Ellis
authors.forename: M J
authors.initials: MJ
authors.lastname: Evans
authors.forename: R
authors.initials: R
authors.lastname: Smith
authors.forename: R A
authors.initials: RA
authors.lastname: Donald
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000378:metabolism; D003346:Corticotropin-Releasing Hormone / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006736:Horses; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D012756:Sheep; D013045:Species Specificity
chemical_list: D002352:Carrier Proteins; C067133:corticotropin releasing factor-binding protein; D003346:Corticotropin-Releasing Hormone