[Effects of indomethacin in guinea pig cochlea: researches on audiology, morphology and cochlear blood flow].
Prostaglandins are known to be widely distributed in mammal tissues with diverse and potent biological activities. Indomethacin is a well-known cyclooxygenase inhibitor of prostaglandin synthesis. However, no paper appeared in the literature with regard to the effects of indomethacin on inner ear hearing function and cochlear lateral wall blood flow (LWBF). The present project was undertaken to investigate the effect of super-tolerated doses of indomethacin on audiology, morphology and LWBF of cochlea when that endogenous prostaglandin synthesis was greatly suppressed. In drug-treated group, super-tolerated doses of indomethacin (100 mg/kg) were given iv. to 29 guinea pigs. The compound action potentials (CAP) were monitored before and after drug administration till 180 minutes. The morphological changes of the organ of Corti were examined with scanning electron microscope, and that of stria vascularis with light and transmission electron microscopes. The LWBF were measured by biomicrosphere technique. The data were compared to those of the control group. Results showed no statistically significant changes of amplitude and threshold of CAP after indomethacin injection. No obvious morphological damage was found in the organ of Corti and stria vascularis except that a few of myelin figure were observed in marginal cell. LWBF was not decreased at 30 and 120 minutes after indomethacin injection. The study suggests that therapeutic doses of indomethacin do not damage cochlea, and inner ear hearing function is not severely interfered with when endogenous PGs synthesis is greatly suppressed, at least over the short time.
Kong
W
W
Xiehe Hospital of Tongji Medical University, Wuhan.
D000818:Animals; D001300:Audiometry, Evoked Response; D003051:Cochlea / Q000098:blood supply / Q000187:drug effects* / Q000502:physiology; D005072:Evoked Potentials, Auditory; D006168:Guinea Pigs; D007213:Indomethacin / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D009925:Organ of Corti / Q000648:ultrastructure; D012039:Regional Blood Flow / Q000187:drug effects
D007213:Indomethacin