Demonstration of immuno-bound alkaline phosphatase by a direct lead method. Optimization of reaction parameters by microdensitometry using computer-aided image processing.
Antigens labelled by the immunohistochemical alkaline phosphatase anti-alkaline phosphatase (APAAP) method can be visualized by a lead capture technique. Optimal conditions of the reaction, evaluated by microdensitometry using computer-aided image processing, are described.
Bock
R
R
Fachrichtung 3.1 Anatomie im Fachbereich Theoretische Medizin, Universität des Saarlandes, Homburg/Saar, Germany.
Chakraverty-Werner
K
K
Herth
G
G
Liu
N
N
Ostermann
E
E
Töx
U
U
D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D000906:Antibodies; D003720:Densitometry / Q000379:methods; D007031:Hypothalamus / Q000166:cytology* / Q000201:enzymology; D007150:Immunohistochemistry / Q000379:methods; D009412:Nerve Fibers / Q000201:enzymology / Q000648:ultrastructure*; D009481:Neurophysins / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014667:Vasopressins / Q000032:analysis
D000906:Antibodies; D009481:Neurophysins; D014667:Vasopressins; D000469:Alkaline Phosphatase