Inhibitory effects of recombinant human cystatin C on human coronaviruses.
Cystatin C, a potent inhibitor of cysteine proteases such as papain and cathepsin B, was examined for its effect on human coronaviruses OC43 and 229e. Both viruses were greater than 99% inhibited by 0.1 mM inhibitor. Endpoint titrations showed that inhibiting activity paralleled that of leupeptin, a serine and cysteine protease inhibitor, and indicated that 1 to 2 microM inhibitor, slightly above physiologic levels, was effective.
Collins
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Department of Microbiology, State University of New York, Buffalo 14214.
Grubb
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D000998:Antiviral Agents / Q000494:pharmacology*; D002401:Cathepsin B / Q000494:pharmacology; D002478:Cells, Cultured; D003332:Coronaviridae / Q000187:drug effects*; D003513:Cycloheximide / Q000494:pharmacology; D055316:Cystatin C; D015891:Cystatins / Q000494:pharmacology*; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D007976:Leupeptins / Q000494:pharmacology; D010206:Papain / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; C525030:CST3 protein, human; D055316:Cystatin C; D015891:Cystatins; D007976:Leupeptins; D011994:Recombinant Proteins; D003513:Cycloheximide; D002401:Cathepsin B; D010206:Papain; C032854:leupeptin