[Effect of flanking regions on the expression of the human interleukin-2 chromosomal gene in a murine myeloma cell line].
A human interleukin-2 (IL-2) gene was isolated from genomic DNA library. The isolated gene with 5'- and 3'-flanking sequences of various lengths was inserted into plasmids derived from the retroviral vector pPSneo. The recombinant plasmids were transfected into myeloma X63Ag8-653 cells. The transfected cells, harbouring the IL-2 gene with the shortened (to position -165) or totally deleted 5'-flanking sequence, constitutively expressed biologically active IL-2. Deletion of 3'-flanking region on did not affect the IL-2 expression.
Anikeeva
N N
NN
Vinogradova
T V
TV
Dement'eva
E S
ES
Ibragimov
A R
AR
D000818:Animals; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D002877:Chromosomes, Human; D015870:Gene Expression; D015698:Genomic Library; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000235:genetics; D010957:Plasmids; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014162:Transfection
D007376:Interleukin-2
Vliianie flankiruiushchikh oblasteĭ na ékspressiiu khromosomnogo genainterleĭkina-2 cheloveka v myshinoĭ mielomnoĭ kletochnoĭ linii.