Tg (9 HSA-MYC), a homozygous lethal insertion in the mouse.
Transgenic mice generated with different DNA sequences were surveyed for possible homozygous mutant phenotypes. We found an embryonic lethal mutation in the transgenic mouse strain (MT-MYC12.4) containing the human c-myc gene. Embryos homozygous for the transgene die shortly after implantation. The strain MT-MYC12.4 carries approximately 50 tandem copies of the recombinant plasmid sequence. The 3' flanking sequence has been cloned and analyzed. It contains a unique sequence that has been conserved during evolution and maps to Chromosome (Chr) 9. This mutant has been designated Tg 9 (HSA-MYC).
Tutois
S
S
Unité de Génétique des Mammifères, Institut Pasteur, Paris, France.
Salaun
J
J
Mattei
M G
MG
Guénet
J L
JL
D000818:Animals; D001483:Base Sequence; D002503:Centromere; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004622:Embryo, Mammalian / Q000033:anatomy & histology; D005314:Embryonic and Fetal Development / Q000235:genetics*; D005804:Genes, Lethal / Q000235:genetics*; D006720:Homozygote; D051379:Mice / Q000196:embryology / Q000235:genetics*; D008822:Mice, Transgenic / Q000196:embryology / Q000235:genetics; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional