Mg2+ influx into Mg(2+)-depleted reticulocytes.
Mg(2+)-depleted rat reticulocytes reincubated in Mg(2+)-containing media expressed net Mg2+ influx, which was the same in NaCl, choline Cl and sucrose medium. Km of Mg2+ influx amounted to 1.2 mM and Vmax to 0.9 mmol/l cells x h. Mg2+ influx into reticulocytes was inhibited by amiloride, quinidine and imipramine. Mg2+ uptake together with Cl- for charge compensation could be excluded. However, the cation which must be exchanged for Mg2+ in Mg2+ uptake could not be identified because of the low rate of Mg2+ influx.
Günther
T
T
Institute of Molecular Biology and Biochemistry, Free University of Berlin, FRG.
Vormann
J
J
D000818:Animals; D002478:Cells, Cultured; D008274:Magnesium / Q000008:administration & dosage / Q000097:blood*; D008275:Magnesium Deficiency / Q000097:blood / Q000139:chemically induced; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012156:Reticulocytes / Q000737:chemistry / Q000378:metabolism*
D008274:Magnesium