title: [Cloning a species-specific DNA probe from Fusarium oxysporum fungus].
abstract: A method to obtain genus-specific DNA probes is suggested. It consists of specific amplification of the intergenic spacer between the 18S and 5.8S ribosomal RNA genes, using primers deduced from conservative ribosomal DNA sequences. The utility of the method is demonstrated on isolation of the 209 b.p. spacer fragment from the genomic DNA of a plant pathogenic fungus Fusarium oxysporum.
authors.forename: B K
authors.initials: BK
authors.lastname: Irisbaev
authors.forename: A S
authors.initials: AS
authors.lastname: Kraev
authors.forename: G E
authors.initials: GE
authors.lastname: Pozmogova
authors.forename: A A
authors.initials: AA
authors.lastname: Abdukarimov
authors.forename: K G
authors.initials: KG
authors.lastname: Skriabin
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D004587:Electrophoresis, Agar Gel; D005670:Fusarium / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010957:Plasmids; D013045:Species Specificity
chemical_list: D015342:DNA Probes
vernacular_title: Klonirovanie rodospetsificheskogo DNK-zonda iz griba Fusarium oxysporum.