Partial purification of alpha-hemolysin in Escherichia coli.
The present paper describes isolation and purification of alpha-hemolysin of Escherichia coli. The optimum production medium was found to be the Todd-Hewitt broth. Out of thirteen fractions obtained after separation on Sephadex G-200, two fractions possessed the highest relative specific activity.
Nistiar
F
F
Department of Microbiology, Faculty of Medicine, P.J. Safárik University, Kosice, Czechoslovakia.
Janigová
V
V
Durovicová
J
J
Puzová
H
H
D000818:Animals; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel; D003470:Culture Media; D004912:Erythrocytes / Q000382:microbiology; D004926:Escherichia coli / Q000737:chemistry* / Q000254:growth & development; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans
D001426:Bacterial Proteins; D003470:Culture Media; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli