High-performance liquid chromatographic determination of iothalamic acid in human plasma and urine.
A simple, rapid and sensitive method for the determination of iothalamic acid (IA) in both plasma and urine is reported. After extraction with ethyl acetate, IA was determined by strong anion-exchange high-performance liquid chromatography with ultraviolet detection at 254 nm. The lower limit of detection was 0.5 micrograms/ml. The average recovery was 73 and 57% from plasma and urine, respectively. Linearity was found over the investigated concentration range (up to 500 micrograms/ml for plasma and up to 10.0 mg/ml for urine). The reproducibility of the technique was good (coefficient of variation less than 6%) as was the precision and accuracy (coefficient of variation less than 2.5%). No interference from endogenous substances or any of the common drugs tested was found.
Gaspari
F
F
Mario Negri Institute for Pharmacological Research, Laboratory of Clinical Pharmacokinetics, Bergamo, Italy.
Mainardi
L
L
Ruggenenti
P
P
Remuzzi
G
G
D000085:Acetates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007483:Iothalamic Acid / Q000378:metabolism*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000085:Acetates; D007483:Iothalamic Acid; C007650:ethyl acetate