Use of factor VII-deficient beagles bred by artificial insemination to evaluate mechanisms of thrombosis associated with prothrombin complex concentrates.
Artificial insemination with frozen semen was used to breed beagles homozygous for a deficiency in clotting factor VII, with plasma concentrations of 2-3% of normal adult beagle pooled plasma. The factor VII-deficient beagles were used to determine if factor VII activity has a role in the thrombogenic activity of prothrombin complex concentrates (PCCs) containing factors II, IX and X by evaluating changes in selected indicators of thrombogenicity. No differences were seen between the responsiveness of the factor VII-deficient and normal beagles, indicating that the thrombogenic activities of PCCs are mediated via effects on the intrinsic clotting system.
Ferguson
J M
JM
Wellcome Surgical Institute, University of Glasgow.
MacGregor
I R
IR
McLaughlin
L F
LF
Prowse
C V
CV
D000818:Animals; D001779:Blood Coagulation Factors / Q000009:adverse effects*; D004195:Disease Models, Animal; D004285:Dogs / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D005168:Factor VII Deficiency / Q000097:blood / Q000235:genetics*; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D005344:Fibrinopeptide A / Q000032:analysis; D010314:Partial Thromboplastin Time; D013927:Thrombosis / Q000097:blood / Q000209:etiology*
D001779:Blood Coagulation Factors; D005338:Fibrin Fibrinogen Degradation Products; D005344:Fibrinopeptide A; C025667:prothrombin complex concentrates