[The effect of centrosome UV microbeam irradiation on cell behavior. II. The radiation sequelae in the anaphase: the completion of division and the fate of the interphase cell].
Ultraviolet (280 nm) microbeam irradiation of the centrosome (spindle pole) in the early anaphase slows down and then stops chromosome movement towards the irradiated pole. This happens as a result of rapid (in 1-2 min) disorganization of the half-spindle. Chromosome movement towards the opposite pole continues normally. Irradiation of the centrosome also affects cystotomy--the residual body is formed later than in the normal cell. In some cases additional constrictions are formed or the cytoplasm starts blebbing. Immediately after division the microtubule network in two daughter cells (one of them with irradiated centrosome) is similar. Two hours later in the irradiated cell the amount of microtubules is often less than in the sister cell. Incubation with nocodazole (0.5-1.5 h, 0.15 microgram/ml) shows that in the irradiated cells microtubules radiating from the centrosome are practically absent. Irradiation of other regions of the cytoplasm does not cause any of the effects described above.
Uzbekov
R E
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Vorob'ev
I A
IA
D000705:Anaphase / Q000187:drug effects / Q000528:radiation effects*; D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D002875:Chromosomes / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D003593:Cytoplasm / Q000187:drug effects / Q000528:radiation effects* / Q000648:ultrastructure; D007399:Interphase / Q000187:drug effects / Q000528:radiation effects*; D008870:Microtubules / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D015739:Nocodazole / Q000494:pharmacology; D013997:Time Factors; D014466:Ultraviolet Rays
D015739:Nocodazole
Vliianie UF-mikrooblucheniia tsentrosomy na povedenie kletok. II. Posledstviia oblucheniia v anafaze: zavershenie deleniia i sud'ba interfaznoĭ kletki.