PAF antagonists do not modify IgE antibody production in mice.
The effect of selective PAF antagonists on the in vivo production of IgE antibodies was investigated. The anti-ovalbumin IgE antibody content was estimated by passive cutaneous anaphylactic reaction (PCA) in the plasma of Balb/c mice 10 days after immunization with ovalbumin and alum. The PAF antagonists, BN 52021 (5 mg/kg, ip), BN 50730 (20 mg/kg, po), WEB 2086 (2 mg/kg, ip) and WEB 2170 (5 mg/kg, ip) were administered 1 h before immunization and twice a day for 8 days thereafter. The effect of the antagonists on the PAF-induced vasopermeability was also assayed. In the immunized mice the level of antiovalbumin IgE antibody, estimated by PCA titer, was 1/640. The treatment with the PAF antagonists did not change this level. At the concentrations employed, the antagonists BN 50730, WEB 2086 and WEB 2170 significantly reduced the PAF-induced vascular permeability. These results suggest that PAF does not seem to have a relevant effect on the production of IgE antibodies in vivo in the system used in the present study.
Jancar
S
S
Departamento de Imunologia, Universidade de São Paulo, Brasil.
Riffo-Vasquez
Y
Y
Ferreira
A P
AP
Macedo
M S
MS
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D005260:Female; D007073:Immunoglobulin E / Q000032:analysis / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D010323:Passive Cutaneous Anaphylaxis; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D014654:Vascular Patency / Q000187:drug effects
D000888:Antibodies, Anti-Idiotypic; D010972:Platelet Activating Factor; D007073:Immunoglobulin E; D010047:Ovalbumin