Medium-scale production and purification of monoclonal antibodies in protein-free medium.
Hybridoma cell lines can be adapted to grow in a totally protein-free tissue culture medium and cultured in spinner flasks to generate moderate-to-high quantities of monoclonal antibodies. Such antibodies are easily purified by ammonium sulfate precipitation. This system was shown to be useful for growth of 23 different hybridoma cell lines from different sources to yield an average of 40 mg of highly purified antibody per liter of tissue culture medium.
Tarleton
R L
RL
Department of Zoology, University of Georgia, Athens 30602.
Beyer
A M
AM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002460:Cell Line; D006224:Cricetinae; D016895:Culture Media, Serum-Free; D046508:Culture Techniques / Q000379:methods*; D006825:Hybridomas / Q000276:immunology*; D051379:Mice; D051381:Rats
D000911:Antibodies, Monoclonal; D016895:Culture Media, Serum-Free