Inverse correlation between fibronectin mRNA levels and the metastatic potential of two murine mammary adenocarcinomas.
The ability of cells to metastasize is thought to be related with the loss of the capacity to synthesize and secrete fibronectin (FN). We found that primary cultures from 2 murine adenocarcinoma tumors, M3 of moderate metastasizing ability and the highly metastasizing MM3, had a dramatic difference in the immunohistochemical expression of FN. It was shown, as assayed by the S1 mapping technique that, while total FN mRNA was fairly abundant in M3 cells, it was almost undetectable in MM3 cells. This difference is not due to a significant deletion of the FN gene in MM3 cells.
Muro
A
A
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Buenos Aires, Argentina.
Puricelli
L
L
Kornblihtt
A R
AR
Bal de Kier Joffé
E
E
D000230:Adenocarcinoma / Q000737:chemistry*; D000818:Animals; D015139:Blotting, Southern; D005110:Extracellular Space / Q000737:chemistry; D005353:Fibronectins / Q000032:analysis / Q000235:genetics*; D015674:Mammary Neoplasms, Animal / Q000737:chemistry*; D051379:Mice; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000032:analysis*
D005353:Fibronectins; D012333:RNA, Messenger