Pentamidine sensitivity and resistance in Saccharomyces cerevisiae as a model for pentamidine effects on Pneumocystis carinii.
Pentamidine isethionate inhibits growth of Saccharomyces cerevisiae. Inhibition requires lower drug concentrations under respiratory than fermentative growth conditions. Pentamidine-resistant strains bear heritable resistance mutations. Tested mutations are dominant to wild type sensitivity. These mutations may identify cellular targets of pentamidine and potential mechanisms of fungal pentamidine resistance.
Hatfield
C
C
T.H. Morgan School of Biological Sciences, University of Kentucky, Lexington 40506-0225.
Kasarskis
A
A
Staben
C
C
D002413:Cations, Divalent / Q000378:metabolism; D004352:Drug Resistance, Microbial / Q000235:genetics; D008954:Models, Biological; D009154:Mutation; D010419:Pentamidine / Q000378:metabolism / Q000494:pharmacology*; D011010:Pneumocystis / Q000187:drug effects* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics
D002413:Cations, Divalent; D010419:Pentamidine