Flow injection immunoassay using a protein A immunoreactor.
Competitive immunoassays have been developed for the immunosuppressant cyclosporin A and the anti-asthmatic drug theophylline utilizing identical controlled pore glass-protein A microcolumns and flow injection techniques. For cyclosporin A the assay was based on a monoclonal antibody with fluorescence detection whilst for theophylline sheep antiserum and electrochemical detection was used.
Miller
J N
JN
Department of Chemistry, Loughborough University, Leicestershire, UK.
Palmer
D A
DA
French
M T
MT
D016572:Cyclosporine / Q000032:analysis; D004563:Electrochemistry; D017022:Flow Injection Analysis; D007118:Immunoassay / Q000379:methods*; D013050:Spectrometry, Fluorescence; D013205:Staphylococcal Protein A / Q000276:immunology*; D013806:Theophylline / Q000032:analysis
D013205:Staphylococcal Protein A; D016572:Cyclosporine; D013806:Theophylline