Prostaglandin E2 (PGE2) effect upon ICP-23 human pulmonary fibroblasts growth in culture and on measles virus multiplication in this cell type.
The influence of PGE2 in different concentrations (10(-4)M, 10(-6)M, 10(-8)M) and of 1 mM AMPc upon ICP-23 human pulmonary fibroblasts and also the influence of PGE2 upon measles virus multiplication in the same cell type were studied. PGE2 inhibited fibroblasts growth in all administered concentrations, depending on them. AMPc adding to human fibroblasts in culture progressively stimulated cells growth in the first 24 hours, produced a steady growth during 24-48-hour interval and slightly inhibited cellular divisions between 48 and 72 hours. PGE2, added in the same concentrations to measles virus--infected ICP-23 cells, concomitantly with virus administration and after 1 hour of viral adsorption influenced viral multiplication, depending on substance concentration and culturing period. Obtained data suggested that PGE2 in physiological concentrations (10(-6)M, 10(-8)M) initially has a weak inhibitory effect (titration after 6 days), but then stimulates viral production (9 days). The initial inhibition is more marked when the substance is added concomitantly with virus administration.
Babeş
L
L
Cantacuzino Institute, Bucharest, Romania.
Orăşanu
M
M
Petraşincu
D
D
Teletin
N
N
D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects; D015232:Dinoprostone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D008459:Measles virus / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects*
D015232:Dinoprostone