title: Immunogenicity of hepatitis B surface antigen derived from the baculovirus expression vector system: a mouse potency study.
abstract: A standard mouse potency test was performed to evaluate the immunogenicity of recombinant hepatitis B surface antigen (HBsAg) produced in the baculovirus/insect cell expression system. Groups of NIH Swiss mice were immunized with serial four-fold amounts of either baculovirus-derived HBsAg adsorbed to aluminum sulfate or a commercially available yeast-derived recombinant HBsAg vaccine preparation. Results from these experiments showed that the effective dose of baculovirus- and yeast-derived HBsAg vaccine preparations necessary to seroconvert 50% of the animals were similar. The duration of the antibody response to HBsAg was studied in mice immunized with the highest doses of the two recombinant vaccine preparations 3 and 6 months after injection. No decrease in the anti-HBs response was observed 6 months after injection. No decrease in the anti-HBs response was observed 6 months after immunization with either of the two vaccine preparations. These results indicate that the baculovirus-derived recombinant HBsAg could serve as an alternative vaccine candidate for hepatitis B virus.
authors.affiliation: Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78228-0147.
authors.forename: R
authors.initials: R
authors.lastname: Attanasio
authors.forename: R E
authors.initials: RE
authors.lastname: Lanford
authors.forename: D
authors.initials: D
authors.lastname: Dilley
authors.forename: G W
authors.initials: GW
authors.lastname: Stunz
authors.forename: L
authors.initials: L
authors.lastname: Notvall
authors.forename: A B
authors.initials: AB
authors.lastname: Henderson
authors.forename: R C
authors.initials: RC
authors.lastname: Kennedy
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D005260:Female; D005822:Genetic Vectors; D006510:Hepatitis B Antibodies / Q000096:biosynthesis; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D051379:Mice; D014614:Vaccines, Synthetic / Q000032:analysis / Q000276:immunology; D014761:Viral Hepatitis Vaccines / Q000032:analysis / Q000276:immunology
chemical_list: D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D014614:Vaccines, Synthetic; D014761:Viral Hepatitis Vaccines