In vitro combination of high dose busulfan with radiotherapy on medulloblastoma cells: additive effect without potentiation.
The aim of this in-vitro study was to evaluate the combination of busulfan with radiotherapy on TE-671 human medulloblastoma cells since unexpected clinical toxicity of busulfan was reported during the treatment of brain tumors, suggesting a possible radiopotentiation. The cytotoxicity of busulfan was determined by using colony forming assays, and doses inducing growth inhibitions of 10, 20 and 50% were selected to be tested in association: 6, 12 and 32 mumol/l for busulfan and 0.5, 1 and 3 Gy for irradiation. All possible combinations were considered within this frame and the results showed that the combination of busulfan with radiotherapy exerted an additive effect without potentiation.
Merlin
J L
JL
Centre Alexis Vautrin, Vandoeuvre-Les-Nancy, France.
Chastagner
P
P
Marchal
C
C
Weber
B
B
Bey
P
P
D002066:Busulfan / Q000008:administration & dosage*; D002460:Cell Line; D002528:Cerebellar Neoplasms / Q000473:pathology* / Q000628:therapy; D003114:Colony-Forming Units Assay; D003131:Combined Modality Therapy; D006801:Humans; D008527:Medulloblastoma / Q000473:pathology* / Q000628:therapy; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000528:radiation effects
D002066:Busulfan