title: Zeta potential of human X- and Y-bearing sperm.
abstract: The zeta potential of human X- and Y-bearing sperm was measured by two different methods: (i) using an electrophoretic light scattering spectrophotometer, and (ii) using a laser-rotating prism. The X- and Y-bearing sperm were separated by free-flow electrophoresis, and their purities were determined by staining for the F-body using quinacrine mustard. The zeta potential of the sperm in the fraction containing more than 80% Y-bearing sperm was approximately -16 mV, whereas that of sperm in the fraction containing more than 95% X-bearing sperm was approximately -20 mV. In other words, the net negative-charge on the cell surface of human X-bearing sperm is higher than that of Y-bearing sperm.
authors.affiliation: Department of Biology, College of Arts and Sciences, University of Tokyo, Japan.
authors.forename: S A
authors.initials: SA
authors.lastname: Ishijima
authors.forename: M
authors.initials: M
authors.lastname: Okuno
authors.forename: H
authors.initials: H
authors.lastname: Mohri
mesh_terms: D002469:Cell Separation; D004594:Electrophysiology; D006801:Humans; D008027:Light; D008297:Male; D012542:Scattering, Radiation; D013053:Spectrophotometry; D013094:Spermatozoa / Q000502:physiology* / Q000648:ultrastructure; D014960:X Chromosome; D014998:Y Chromosome