[Integration of hybrid plasmids-derivatives of staphylococcal plasmid pE3692 and pBR322 vector with Bacillus subtilis chromosome].
Bacillus subtilis is a nonpathogenic microorganism which certainly for also that feature is widely used in several biotechnological processes. Certainly, because of that an elaboration of gene cloning methods in Bacillus subtilis cells is of great significance both from scientific and practical point of view. It was found hybrid plasmids constructed by ligation of pE3692 and pE3692-9 staphylococcal plasmids with pBR322 vector can be integrated with B. subtilis chromosome. Selection of cells in the presence of erythromycin at 50 degrees C allows to isolate clones containing exclusively integrated plasmids. Growth rate of cells containing these plasmids in the selective medium is the same or slightly higher at 50 degrees C than at 37 degrees C. It seems that hybrid plasmid tested pBE4 and pBE91 after appropriate reconstruction in vitro could serve as vectors for B. subtilis gene cloning.
Wieckiewicz
J
J
Zakład Mikrobiologii i Immunologii Instytutu Biologii Molekularnej UJ Krakowie.
Wójcik
K
K
D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics* / Q000648:ultrastructure; D002875:Chromosomes / Q000187:drug effects / Q000648:ultrastructure*; D003001:Cloning, Molecular / Q000187:drug effects / Q000379:methods; D003470:Culture Media; D004917:Erythromycin / Q000494:pharmacology; D006824:Hybridization, Genetic / Q000187:drug effects / Q000235:genetics*; D066298:In Vitro Techniques; D010957:Plasmids / Q000187:drug effects / Q000235:genetics*; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics*; D013696:Temperature; D014170:Transformation, Genetic / Q000187:drug effects / Q000235:genetics*
D003470:Culture Media; D004917:Erythromycin
Integracja do chromosomu Bacillus subtilis hybrydowych plazmidów pochodnych gronkowcowego plazmidu pE3692 i wektora pBR322.