title: Protein kinase C-beta-like immunoreactivity and phosphorylation dependent immunoreactivity of neurofilaments in developing, adult and aged rat peripheral neurons.
abstract: The localizations of protein kinase C-beta-like immunoreactivity (PKC-beta-LI) and phosphorylated neurofilament-like immunoreactivity (PNF-LI) were studied in developing (E12-P28), adult (3-month-old) and aged (15- and 30-month-old) rat dorsal root (DRG) and superior cervical (SCG) ganglia. A close correlation was found between the localization of PKC-beta-LI and PNF-LI in all age groups. PKC-beta-LI was already present in the rat DRG at the time of the appearance of PNF-LI (E12) and after E15 both PKC-beta-and PNF-LI were found in the same subpopulation of neuronal perikarya. Aging had no effect on the distribution or intensity of either PKC-beta or PNF-LI. In the SCG neurons no association was observed between immunoreactivity and accumulation of lipopigments, but in the DRG neurons, lipopigment accumulation was seen in PKC-beta and PNF-immunoreactive large neurons. The results suggest that PKC-beta may regulate the time of appearance and phosphorylation state of some forms of phosphorylated neurofilaments during development. The results also show that no major change occurs in the amount or distribution of PNFs during normal aging.
authors.affiliation: Department of Public Health, University of Tampere, Finland.
authors.forename: R
authors.initials: R
authors.lastname: Roivainen
authors.forename: J
authors.initials: J
authors.lastname: Koistinaho
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D005727:Ganglia, Spinal / Q000254:growth & development / Q000378:metabolism; D005728:Ganglia, Sympathetic / Q000254:growth & development / Q000378:metabolism; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000378:metabolism*; D008297:Male; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D011493:Protein Kinase C