Binding competition of okadaic acid derivatives to anti-okadaic acid antibody.
The serologic activities of structurally related okadaic acid derivatives have been determined. Binding of [3H]okadaic acid to rabbit anti-okadaic acid is inhibited with equal effectiveness by okadaic acid, dinophysistoxin-1, acanthifolicin, okadaic acid tetramethyl ether, and okadaic acid spiroketal II. Okadaic acid spiroketal I, which lacks the F- and G-rings of okadaic acid, inhibits serologic binding about 60 times less effectively. The F- and G-rings of okadaic acid may comprise part of the epitopes recognized by some of the polyclonal antibodies.
Yatsunami
J
J
Cancer Prevention Division, National Cancer Center Research Institute, Tokyo, Japan.
Fujiki
H
H
Suganuma
M
M
Nishiwaki
S
S
Ojika
M
M
Yamada
K
K
Levine
L
L
D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D004988:Ethers, Cyclic / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D019319:Okadaic Acid / Q000031:analogs & derivatives; D011714:Pyrans / Q000276:immunology / Q000302:isolation & purification; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D013141:Spiro Compounds / Q000494:pharmacology
D004988:Ethers, Cyclic; D011714:Pyrans; D013141:Spiro Compounds; D019319:Okadaic Acid; C051904:dinophysistoxin 1; C065560:acanthifolicin