[Investigation on the metabolism of dehydrochloromethyltestosterone in man].
A GC-MS method for the investigation on the metabolism of dehydrochloromethyltestosterone (DHCMT) in man is described. Steroids in human urine were adsorbed on a macroporous XAD-2 resin, eluted with methanol, hydrolyzed with glucuronidase, extracted and concentrated for derivatization and then analyzed with GC-MS. DHCMT and its 7 metabolites were detected in urine samples 8 to 30 h after oral administration. Their structures and the variation of their concentration in urine were determined and the possible metabolic pathways of DHCMT were proposed on the basis of GC-MS feature of its metabolites. The specific metabolites and characteristic ions for screening DHCMT positive urine were chosen. Furthermore, the influence of the sample pretreatment methods on GC-MS results has been studied.
Liu
C S
CS
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Zhang
J
J
Zhou
T H
TH
D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D013739:Testosterone / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine
C000430:D-4-chloro-17 beta-hydroxy-3-oxo-17 alpha-methylandrosta-1,4-diene; D013739:Testosterone