[The ghost muscle fiber with thin filaments reconstructed from nonmuscle actin--a model for studying the cytoskeleton using polarization microfluorimetry].
In muscle fibers which are free of myosin, tropomyosin and troponin thin filaments were reconstructed from muscle and non-muscle G-actin modified with 1,5-IAEDANS. Using polarized microfluorimetry it was shown that actin in such filaments maintained the ability to respond to conformational changes during actin interaction with subfragment of myosin (S1). The models of muscle fibers with reconstructed from non-muscle actin thin filaments are supposed to use for investigation of mechanisms of cell cytoskeleton functions with the help of polarized microfluorimetry.
Khoroshev
M I
MI
Borovikov
Iu S
IuS
D008841:Actin Cytoskeleton / Q000648:ultrastructure*; D000199:Actins / Q000302:isolation & purification / Q000648:ultrastructure*; D000818:Animals; D003584:Cytological Techniques; D003599:Cytoskeleton / Q000648:ultrastructure*; D005454:Fluorescence Polarization / Q000379:methods; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D009132:Muscles / Q000648:ultrastructure*; D015879:Myosin Subfragments / Q000302:isolation & purification / Q000648:ultrastructure; D009282:Naphthalenesulfonates; D011487:Protein Conformation; D011817:Rabbits; D013552:Swine
D000199:Actins; D005456:Fluorescent Dyes; D015879:Myosin Subfragments; D009282:Naphthalenesulfonates; C006421:1,5-I-AEDANS
Tenevoe myshechnoe volokno s rekonstruirovannymi iz nemyshechnogo aktina tonkimi nitiami--model' dlia izucheniia tsitoskeleta s pomoshch'iu poliarizatsionnoĭ mikrofluorimetrii.