title: Analysis of pyruvic acid acetal containing polysaccharides by methanolysis and reductive cleavage methods.
abstract: The mass spectra of permethylated methyl 4,6-O-(1-carbomethoxyethylidene)-D-hexopyranoside and 1,5-anhydro-D-hexitol of glucose, galactose, and mannose and permethylated methyl 5,6-O-(1-carbomethoxyethylidene)-D-galactofuranoside and 1,4-anhydro-D-galactitol have been determined. The stability of each compound toward methanolysis and reductive cleavage is discussed. These techniques permit the identification of the acetalic linkages of pyruvic acid present in polysaccharides.
authors.affiliation: Laboratoire de Chimie Biologique, Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France.
authors.forename: T
authors.initials: T
authors.lastname: Fontaine
authors.forename: F
authors.initials: F
authors.lastname: Talmont
authors.forename: G G
authors.initials: GG
authors.lastname: Dutton
authors.forename: B
authors.initials: B
authors.lastname: Fournet
mesh_terms: D000107:Acetylation; D001407:Bacillus / Q000737:chemistry*; D002240:Carbohydrate Sequence; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D007711:Klebsiella pneumoniae / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry; D013058:Mass Spectrometry; D000432:Methanol; D008745:Methylation; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D011773:Pyruvates / Q000032:analysis*; D013402:Sugar Alcohols / Q000737:chemistry
chemical_list: D011135:Polysaccharides, Bacterial; D011773:Pyruvates; D013402:Sugar Alcohols; C064986:pyruvic acid acetal; D005947:Glucose; D008358:Mannose; D005690:Galactose; D000432:Methanol