title: Microbial degradation of 2 alpha, 3 alpha-dihydroxy-5 alpha-cholestan-6-one by Mycobacterium vaccae.
abstract: 2 alpha,3 alpha-Dihydroxy-5 alpha-cholestan-6-one (3), which had the substitution pattern of brassinosteroids in the A/B-ring moiety, was transformed by Mycobacterium vaccae to give 2 alpha,3 alpha,6 alpha-trihydroxy-5 alpha-androstan-17-one (4) and 2 alpha-hydroxyandrost-4-ene-3,17-dione (5). The structures of these compounds were determined by spectroscopic methods, especially 1H nuclear magnetic resonance studies.
authors.affiliation: Institute of Plant Biochemistry, Halle, Weinbergweg, Germany.
authors.forename: H M
authors.initials: HM
authors.lastname: Vorbrodt
authors.forename: G
authors.initials: G
authors.lastname: Adam
authors.forename: A
authors.initials: A
authors.lastname: Porzel
authors.forename: C
authors.initials: C
authors.lastname: Hörhold
authors.forename: S
authors.initials: S
authors.lastname: Dänhardt
authors.forename: K H
authors.initials: KH
authors.lastname: Böhme
mesh_terms: D001711:Biotransformation; D002778:Cholestanones / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009161:Mycobacterium / Q000378:metabolism*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002778:Cholestanones; C075232:2,3-dihydroxycholestan-6-one