Evidence for nucleating microtubules in microtubular associations and for an opening polarity under colchicine action.
Bundles of microtubular structures appear in the cytoplasm of germinal cells of the African frog Dicroglossus occipitalis. They are made of several associated microtubules. Every bundle contains one normal singlet and numerous arch-shaped microtubular structures growing in all directions from the singlet wall. The walls of these microtubules are shown to contain 10 to 13 protofilaments. Attempts made with colchicine point out their susceptibility to this antimitotic drug. The formation and opening of these microtubular structures give evidence of complex organization.
Siau
Y
Y
Department of Biology, Faculty of Sciences, C. A. Diop University of Dakar, Sénégal.
Wane
A M
AM
Mattei
X
X
Marchand
B
B
D000818:Animals; D001001:Anura; D003078:Colchicine / Q000494:pharmacology; D008297:Male; D008870:Microtubules / Q000737:chemistry / Q000187:drug effects / Q000648:ultrastructure*; D013087:Spermatids / Q000648:ultrastructure*; D014404:Tubulin / Q000737:chemistry
D014404:Tubulin; D003078:Colchicine