title: Immunoreactive delta sleep-inducing peptide in the rat hypothalamus, pituitary and adrenal gland: effects of adrenalectomy.
abstract: Immunoreactive (IR) delta sleep-inducing peptide (DSIP) was examined by immunocytochemistry in the rat pituitary and adrenal gland and found to be colocalized with IR thyroid-stimulating hormone in the pituitary and with noradrenaline in the adrenal medulla. IR-DSIP was also detectable in nerve fibers in the posterior pituitary. By radioimmunoassay, IR-DSIP was quantified in plasma and tissue extracts after uni- or bilateral adrenalectomy. Significantly elevated plasma levels of IR-DSIP were measured 5 days after bilateral adrenalectomy (p less than 0.001). IR-DSIP was increased (p less than 0.05) in pituitary extracts from bilaterally adrenalectomized rats after 5 days, but not after 14 or 28 days. Sham- and unoperated animals did not significantly differ in plasma or tissue concentration of IR-DSIP. High-performance liquid chromatography of C18 SEP-PAK purified hypothalamus extracts revealed a single peak of IR-DSIP material of lower hydrophobicity than synthetic DSIP. The elevated concentration of IR-DSIP in the rat pituitary and plasma after bilateral adrenalectomy is consistent with the previously suggested role of DSIP to influence the activity of the hypothalamic-pituitary-adrenal axis.
authors.affiliation: Department of Medical Cell Research, University of Lund, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Bjartell
authors.forename: F
authors.initials: F
authors.lastname: Sundler
authors.forename: R
authors.initials: R
authors.lastname: Ekman
mesh_terms: D000311:Adrenal Glands / Q000737:chemistry* / Q000378:metabolism; D000315:Adrenalectomy; D000595:Amino Acid Sequence; D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003346:Corticotropin-Releasing Hormone / Q000032:analysis; D003701:Delta Sleep-Inducing Peptide / Q000032:analysis* / Q000378:metabolism; D007031:Hypothalamus / Q000737:chemistry* / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013972:Thyrotropin / Q000032:analysis; D014018:Tissue Distribution
chemical_list: D001127:Arginine Vasopressin; D003701:Delta Sleep-Inducing Peptide; D013972:Thyrotropin; D003346:Corticotropin-Releasing Hormone