title: [Method of diagnosing aneuploidies using in situ hybridization: analysis of interphase nuclei].
abstract: The possibility of determining chromosomal sex using in situ hybridization of X-specific alphoid DNA probe with interphase human nuclei is studied. Total number of nuclei under study being 14806, more than 85 percent of female ones and more than 95 per cent of male ones are shown to contain two and one grain clusters, respectively. The minor nuclear classes, i.e. containing other numbers of grain clusters, may reflect the effects of polyploidization and cluster aggregation resulting from spatial chromosome distribution. Therefore the positive analysis of sex chromosome constitution on the base of the applied method is possible.
authors.forename: S G
authors.initials: SG
authors.lastname: Vorsanova
authors.forename: Iu B
authors.initials: IuB
authors.lastname: Iurov
authors.forename: G V
authors.initials: GV
authors.lastname: Deriagin
authors.forename: I V
authors.initials: IV
authors.lastname: Solov'ev
authors.forename: G A
authors.initials: GA
authors.lastname: Bytenskaia
mesh_terms: D000782:Aneuploidy; D002467:Cell Nucleus; D015342:DNA Probes; D005260:Female; D006801:Humans; D007399:Interphase; D008297:Male; D009693:Nucleic Acid Hybridization; D012730:Sex Chromosomes
chemical_list: D015342:DNA Probes
vernacular_title: K metodike diagnostiki aneuploidiĭ pri pomoshchi gibridizatsii in situ: analiz interfaznykh iader.