High-performance liquid chromatographic method for the determination of a potential memory-enhancing compound (CL 275,838) and its desbenzyl metabolite in rat plasma or serum.
A rapid, selective, precise reversed-phase liquid chromatographic method has been developed for the determination of a potential memory-enhancing agent (CL 275,838) and its main metabolite (CL 286,527) in plasma and serum. The procedure includes isolation of compounds from proteins precipitated with acetonitrile, subsequent resolution by reversed-phase (Whatman Partisphere C8) high-performance liquid chromatography and ultraviolet detection. The assay was linear over the range 0.12-1.25 micrograms/ml of plasma or serum. The detection limit was 0.12 micrograms/ml, using 0.2 ml of plasma or serum. Intra- and inter-day validation studies indicated an acceptable precision and reproducibility of the method within the concentration range investigated, the overall coefficient of variation being less than 10%. The method is currently applied in support of pharmacological and toxicity studies of the compound in rodents.
Guiso
G
G
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Confalonieri
S
S
Caccia
S
S
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008297:Male; D008568:Memory / Q000187:drug effects*; D010879:Piperazines / Q000097:blood* / Q000378:metabolism; D011720:Pyrazoles / Q000097:blood* / Q000378:metabolism; D011743:Pyrimidines / Q000097:blood* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D010879:Piperazines; D011720:Pyrazoles; D011743:Pyrimidines; C074871:CL 275838; C074870:CL 286527