Quick and high-efficiency electroelution of nucleic acid fragments.
We describe a quick, simple, and inexpensive technique for the electroelution of nucleic acid fragments that provides a high yield of DNA by using common laboratory components. The quantity of buffer used for the recovery of nucleic acid fragments is relatively small, the quality of the DNA recovered is relatively high, and more than one electroelution can be carried out at the same time.
Pai
S R
SR
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Alabama 36849.
Bird
R C
RC
D011232:Chemical Precipitation; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005821:Genetic Techniques; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics
D016760:Proto-Oncogene Proteins c-fos; D004247:DNA