Immunohistochemical investigation of autoantibodies to nerve fibers in cardiomyopathy.
The immunofluorescence technique was used to trace auto-antibodies to neural structures in the blood serum of 180 patients with various cardiomyopathies and 20 healthy probands (controls). Incubation of cryostat slices of heart, kidney, spinal cord and medulla oblongata of Wistar-rats or of cell cultures of embryonal spinal cord with the blood serum of patients with cardiomyopathies resulted in immunofluorescence of nerve fibres and neuronal perikaryon. The controls were negative. The question remained unanswered for the nature of the involved antigen. Neurohistochemical findings suggested auto-antibodies in sera of patients to be orientated to cholinergic endings. The authors suggest the immunomorphological approach as practicable for revealing auto-antibodies in neurohistological studies.
Nanaev
A K
AK
L. A. Myasnikov Institute of Clinical Cardiology, USSR Academy of Medical Sciences, Moscow.
Shvalev
V N
VN
Kozlova
M V
MV
Rukosuev
V S
VS
D000818:Animals; D001323:Autoantibodies / Q000097:blood*; D009202:Cardiomyopathies / Q000276:immunology*; D005629:Frozen Sections; D006321:Heart / Q000294:innervation; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000294:innervation; D008526:Medulla Oblongata / Q000276:immunology; D009412:Nerve Fibers / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000276:immunology
D001323:Autoantibodies