[Effect of synthetic protease inhibitor on the oncogenesis of pancreatic cancer in hamsters: study on pancreatic endocrine cells and free radicals].
In order to study the effect of synthetic trypsin inhibitor on the oncogenesis of pancreatic cancer, the histology, the kinetics of the B, A and D cells in the islets of Langerhans and activities of free radical scavengers, superoxide dismutase (SOD), glutathion peroxide (GSH-Px) and malon dialdehyde (MDA) in the tumor bearing tissues were measured in hamsters with pancreatic cancer induced by di-iso-propanol nitrosamine (DIPN) with or without camostat (FOY-305). In DIPN group (DIPN alone), the tubular adenocarcinoma was found in 80%, however, in FOY group (DIPN+FOY-305), papillary adenocarcinoma was found in 91%. In both DIPN and FOY groups, the number of B cells was decreased at 8 weeks and the number of A and D cells was decreased at 16 weeks. Activities of SOD in the tumor and borderzone in DIPN group were significantly lower than those in non-tumor region and normal tissue. However, activities of SOD in the tumor and borderzone in FOY group were higher than those in DIPN group. GSH-Px and MDH levels were significantly higher in FOY group suggesting the involvement in the reaction of free radicals. These results suggest that trypsin inhibitors have a prophylactic effect on the development of pancreatic cancer.
Asano
N
N
First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Manabe
T
T
Yoshimura
T
T
Imanishi
K
K
Tobe
T
T
D000818:Animals; D006224:Cricetinae; D004952:Esters; D016166:Free Radical Scavengers; D005609:Free Radicals; D016670:Gabexate / Q000031:analogs & derivatives*; D005978:Glutathione / Q000378:metabolism; D006146:Guanidines / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000187:drug effects* / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008647:Mesocricetus; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D010545:Peroxides / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000494:pharmacology*
D004952:Esters; D016166:Free Radical Scavengers; D005609:Free Radicals; D006146:Guanidines; D010545:Peroxides; D014361:Trypsin Inhibitors; C034532:camostat; D016670:Gabexate; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione