title: Analysis of carbamazepine and its 10,11-epoxide in serum by direct sample injection using surfactant containing eluents and column switching.
abstract: Column switching is used in conjunction with surfactant containing mobile phases and traditional reverse phase LC columns to provide a highly reproducible and accurate analytical procedure for carbamazepine and its 10,11-epoxide in serum. This approach eliminates the tedious sample preparation steps commonly used in the analysis of drugs by HPLC, while providing a high degree of protein removal prior to the final analysis. Various pre-columns were investigated and a pellicular reverse phase column was found suitable for optimum concentration of drugs and removal of serum proteins. A variety of standard reverse phase columns could be used for the analytical separation. The separation of the drugs could be accomplished with a high degree of reproducibility. Tandem pre-column operation was demonstrated to give a sample throughput of 10 h-1.
authors.affiliation: Waters Chromatography Division of Millipore Corporation, Milford, MA 01757.
authors.forename: S
authors.initials: S
authors.lastname: Schmitz
authors.forename: F V
authors.initials: FV
authors.lastname: Warren
authors.forename: B A
authors.initials: BA
authors.lastname: Bidlingmeyer
mesh_terms: D002220:Carbamazepine / Q000031:analogs & derivatives* / Q000097:blood*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D016903:Drug Monitoring / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D013501:Surface-Active Agents / Q000032:analysis
chemical_list: D013501:Surface-Active Agents; D002220:Carbamazepine; C012227:carbamazepine epoxide