Microcrystals of the annexin, p68: paracrystal to crystal transition and molecular packing as determined by electron microscopy and image reconstruction.
The calcium-sensitive, membrane-binding annexin, p68, has been crystallized from solutions of polyethylene glycol and ammonium sulfate. Our electron microscopy and X-ray diffraction data indicate that p68 crystals are tetragonal, in space group P4(1), and have unit cell dimensions of a = b = 68.4 A and c = 209.6 A. The mechanism of crystallization from polyethylene glycol involves a transition from a paracrystalline form to ordered crystals by lateral reordering of chains of molecules extended along the c axis. These chains are directional and might reflect a mechanism whereby the two different ends of (chains of) the p68 molecules interact with different membranes.
Gabriel
B L
BL
Department of Pharmacology, University of Virginia, Charlottesville 22901.
Taylor
K
K
Creutz
C E
CE
Kretsinger
R H
RH
D000818:Animals; D017317:Annexin A6; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000648:ultrastructure; D002417:Cattle; D003460:Crystallization; D007091:Image Processing, Computer-Assisted; D008854:Microscopy, Electron; D014961:X-Ray Diffraction
D017317:Annexin A6; D002135:Calcium-Binding Proteins