Application of ion-pair high-performance liquid chromatography to detection of the atypical coproporphyrin isomers II and IV in human faeces.
A highly selective and sensitive method has been developed for the detection of small amounts of the atypical isomers II and IV of coproporphyrin in human faeces. This method combines liquid-liquid extraction and solid-phase sampling techniques using talc and C18-modified silica gel as the sorbents. Simultaneous separation of the four coproporphyrin isomers I-IV was achieved by isocratic ion-pair high-performance liquid chromatography. Stool samples of healthy subjects (n = 12) contained 1.1 +/- 0.4% (mean +/- S.D.) isomer II and 2.2 +/- 0.9% isomer IV of total coproporphyrins. A somewhat higher content of isomer II (2.7%) and isomer IV (5.4%) was found in faeces of a patient suffering from porphyria variegata.
Jacob
K
K
Institut für Klinische Chemie, Universität München, Germany.
Egeler
E
E
Hennel
B
B
Neumeier
D
D
Luppa
P
P
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003306:Coproporphyrins / Q000378:metabolism*; D005243:Feces / Q000737:chemistry*; D011164:Porphyrias / Q000378:metabolism; D012016:Reference Values; D013050:Spectrometry, Fluorescence
D003306:Coproporphyrins; C062430:coproporphyrin IV; C062429:coproporphyrin II