Preparation and surface characterization of carboxymethylchitin-incorporated submicron bilayer-lipid membrane artificial cells (liposomes) encapsulating hemoglobin.
In view of the desirability to increase the survival time of the lipsome-based artificial red blood cells in vivo, bovine hemoglobin-loaded liposomes (LEHb) are incorporated with a polyanionic polymer, a highly substituted type of carboxymethylchitin (CMC). The liposomes are prepared by a modified Reverse Phase Evaporation technique and then purified using a Sepharose 4B column. A comparative study between experimental techniques for the determination of adsorption efficiency suggests that FT-IR spectroscopy gives a more accurate quantitative adsorption index while the chitinase-based enzymatic assay should be used as a qualitative detection tool. The overall composition of the bilayer(s) can be approximated by that of the natural (RBC) membrane in terms of total lipids and carbohydrates at 87.8% (phospholipids and cholesterol) and 12.2% CMC respectively.
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Artificial Cells and Organs Research Centre, McGill University, Montreal, Canada.
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D001672:Biocompatible Materials / Q000737:chemistry*; D001802:Blood Substitutes / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002686:Chitin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D002688:Chitinases; D004337:Drug Carriers; D004339:Drug Compounding; D004910:Erythrocyte Membrane; D006454:Hemoglobins / Q000008:administration & dosage*; D008051:Lipid Bilayers; D008081:Liposomes / Q000737:chemistry*; D008969:Molecular Sequence Data; D013055:Spectrophotometry, Infrared
D001672:Biocompatible Materials; D001802:Blood Substitutes; D004337:Drug Carriers; D006454:Hemoglobins; D008051:Lipid Bilayers; D008081:Liposomes; D002686:Chitin; C044872:O-(carboxymethyl)chitin; D002688:Chitinases