title: A reliable two-hour Staphylococcus plate assay of rifampicin, erythromycin, lincomycin, chloramphenicol and amikacin in human sera.
abstract: A simple and precise agar diffusion microassay was developed for rifampicin (RIF) in serum. The method involved the addition of sera containing RIF (0.125-4.0 micrograms/ml) to wells cut in an agar medium (pH 6.6) surface seeded with an adjusted inoculum of Staphylococcus aureus ATCC 6538 P and incubated for 2h at 40 degrees C. Repetitive assays of sera containing known concentrations of RIF revealed an average mean recovery of 93.6% with a coefficient of variation of 5.7%. This rapid method allowed accurate determination of RIF in the presence of erythromycin, chloramphenicol, lincomycin and amikacin. These four antibiotics were also separately measured in sera by the rapid assay at different pH values.
authors.affiliation: Department of Pharmaceutical Microbiology, Alexandria University, Egypt.
authors.forename: N H
authors.initials: NH
authors.lastname: Fanaki
authors.forename: M A
authors.initials: MA
authors.lastname: el-Nakeeb
mesh_terms: D000583:Amikacin / Q000097:blood / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000097:blood*; D001681:Biological Assay / Q000379:methods*; D002701:Chloramphenicol / Q000097:blood / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004917:Erythromycin / Q000097:blood / Q000494:pharmacology; D006801:Humans; D008034:Lincomycin / Q000097:blood / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D012293:Rifampin / Q000097:blood* / Q000494:pharmacology; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D004917:Erythromycin; D002701:Chloramphenicol; D000583:Amikacin; D008034:Lincomycin; D012293:Rifampin