Human myoblast transplantation: a simple assay for tumorigenicity.
A simple assay for tumorigenicity of myoblasts to be transplanted to Duchenne patients has been developed. The assay is based on culture in a soft agar medium for 2-3 weeks. The tumor cell line forms large cell clusters while the normal myoblasts do not proliferate and remain isolated.
Tremblay
J P
JP
Laboratoire de Neurobiologie, Hôpital de l'Enfant-Jésus, Québec, Canada.
Roy
B
B
Goulet
M
M
D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002999:Clone Cells; D046508:Culture Techniques / Q000379:methods; D006801:Humans; D009132:Muscles / Q000166:cytology* / Q000473:pathology / Q000637:transplantation; D012208:Rhabdomyosarcoma / Q000473:pathology*; D013997:Time Factors; D014407:Tumor Cells, Cultured