Correlation between low-temperature immunosuppression and the absence of unsaturated fatty acid synthesis in murine T cells.
1. Studies were performed to determine if the inability of murine T cells to provide primary helper function at low temperature (27 degrees C) could be correlated with their inability to synthesize unsaturated fatty acids (UFAs). 2. In the absence of exogenous oleic acid (18:1), splenocytes responded to a T-dependent (TD) Ag (trinitrophenyl-keyhole limpet hemocyanin, TNP-KLH) at 37 degrees C but not at 27 degrees C. The addition of 150 microM 18:1 almost completely restored plaque-forming cell (PFC) responses to TNP-KLH at 27 degrees C but markedly suppressed PFC responses to the TD Ag at 37 degrees C. 3. During incubation at 27 degrees C, B cells converted 3- to 5-fold more stearic acid (18:0) to 18:1 and showed a greater accumulation of monounsaturated phospholipid molecular species than did T cells. 4. Following incubation in the presence of a rescuing dose of 18:1 (150 microM), both B and T cells accumulated large amounts of dioleoyl PC. 5. It is proposed that the absence of 18:1 synthesis in T cells is responsible for the unique low temperature susceptibility of this lymphocyte population.
Buttke
T M
TM
Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, NC 27858-4354.
Yang
M C
MC
Van Cleave
S
S
Miller
N W
NW
Clem
L W
LW
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D003080:Cold Temperature / Q000009:adverse effects; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D006462:Hemolytic Plaque Technique; D007108:Immune Tolerance; D051379:Mice; D008807:Mice, Inbred BALB C; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D010743:Phospholipids / Q000378:metabolism; D013229:Stearic Acids / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*
D005231:Fatty Acids, Unsaturated; D009829:Oleic Acids; D010743:Phospholipids; D013229:Stearic Acids; D019301:Oleic Acid; C031183:stearic acid