title: Isolation of ribosomal subunits containing intact rRNA from human placenta: estimation of functional activity of 80S ribosomes.
abstract: We have elaborated a method for the isolation of ribosomal subunits from fresh unfrozen human placenta containing intact rRNA and a complete set of ribosomal proteins. Activity of 80S ribosomes obtained by reassociation of 40S and 60S subunits in nonenzymatic poly(U)-dependent binding of Phe-tRNA(Phe) was equal to 80% (above 1.5 mol [14C]Phe-tRNA(Phe) is coupled to 1 mol of ribosomes). The activity of 80S ribosomes in poly(U)-directed synthesis of polyphenylalanine was tested in a polysome-free protein-synthesizing system from rabbit reticulocytes. About 100 mol of phenylalanine residue was polymerized by a mole of ribosomes at a rate of 0.83 residues per minute in this system (2 h, 37 degrees C).
authors.affiliation: Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk.
authors.forename: N B
authors.initials: NB
authors.lastname: Matasova
authors.forename: S V
authors.initials: SV
authors.lastname: Myltseva
authors.forename: M A
authors.initials: MA
authors.lastname: Zenkova
authors.forename: D M
authors.initials: DM
authors.lastname: Graifer
authors.forename: S N
authors.initials: SN
authors.lastname: Vladimirov
authors.forename: G G
authors.initials: GG
authors.lastname: Karpova
mesh_terms: D000818:Animals; D001665:Binding Sites; D002458:Cell Fractionation; D002474:Cell-Free System; D006801:Humans; D010452:Peptide Biosynthesis; D010455:Peptides; D010920:Placenta / Q000737:chemistry*; D011072:Poly U; D012335:RNA, Ribosomal / Q000302:isolation & purification*; D012360:RNA, Transfer, Phe / Q000737:chemistry; D011817:Rabbits; D012156:Reticulocytes; D012269:Ribosomal Proteins / Q000302:isolation & purification; D012270:Ribosomes / Q000737:chemistry* / Q000502:physiology
chemical_list: D010455:Peptides; D012335:RNA, Ribosomal; D012360:RNA, Transfer, Phe; D012269:Ribosomal Proteins; D011072:Poly U; C012001:polyphenylalanine