Establishment of human myeloma cell line KM-2R and its preliminary application to human-human hybridoma research.
The authors report the establishment of a human myeloma cell line--KM-2R--and its preliminary use in human-human hybridoma research. KM-2R cells are resistant to both 6-TG and ouabain. Their doubling time is about 30 h. They have a modal chromosome number of 81-85. Using ELISA and immunodiffusion techniques, gamma-heavy and kappa-light chains were detected in the concentrated cell culture supernatant. KM-2R cells are sensitive to HAT medium. When fusing with normal human spleen cells, tonsil cells, and B lymphoblasts transformed by EB virus, fusion frequencies of 20-60% resulted.
Guo
Y
Y
Institute of Medical Biology, CAMS, Kunming.
Zhang
H
H
Fan
Y
Y
Lin
K
K
Gong
C
C
Fu
J
J
Guo
R
R
D002455:Cell Division; D002459:Cell Fusion; D006801:Humans; D006825:Hybridomas; D007952:Leukemia, Plasma Cell / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000473:pathology*; D014407:Tumor Cells, Cultured