Determination of nitrate by conversion to nitrite using Paracoccus denitrificans.
A new method of determination of nitrate was developed, utilizing the nitrate reductase activity of Paracoccus denitrificans in which a further reduction of nitrate is blocked either by a mutation affecting formation of cytochromes c or by inhibition of the electron flow to nitrite reductase by mucidin. After deproteinization of the sample with zinc acetate the nitrite produced is determined colorimetrically.
Matchová
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Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czechoslovakia.
Cerná
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Kucera
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D004795:Enzyme Stability; D016296:Mutagenesis; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000378:metabolism*; D009566:Nitrates / Q000032:analysis* / Q000378:metabolism; D009572:Nitrite Reductases / Q000235:genetics / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D010231:Paracoccus denitrificans / Q000235:genetics / Q000378:metabolism*
D009566:Nitrates; D009573:Nitrites; D009565:Nitrate Reductases; D009572:Nitrite Reductases; D050901:Nitrate Reductase