Purification and characterization of a hemolysin of Vibrio mimicus that relates to the thermostable direct hemolysin of Vibrio parahaemolyticus.
A hemolysin (designated Vm-rTDH) from Vibrio mimicus (AQ0915-E13) was purified by ammonium sulfate fractionation and successive column chromatography with DEAE-Sephadex A-25, hydroxyapatite, Mono Q, Superose 12 and Phenyl-Superose. The Mr of the subunit was estimated to be about 22,000 by sodium dodecyl sulfate-slab gel electrophoresis. The isoelectric point of Vm-rTDH was approximately pH 4.9. The hemolytic activity of Vm-rTDH was stable upon heating at 100 degrees C for 10 min, similar to that of the thermostable direct hemolysin (Vp-TDH) of V. parahaemolyticus. Vm-rTDH also showed lytic activities similar to those of Vp-TDH. Immunological cross-reactivity between Vp-TDH and Vm-rTDH was demonstrated by the Ouchterlony double-diffusion test. Thus we conclude that V. mimicus produces a newly discovered type of hemolysin (Vm-rTDH) which is similar to Vp-TDH.
Yoshida
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H
Research Institute for Microbial Diseases, Osaka University, Japan.
Honda
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Miwatani
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D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006460:Hemolysin Proteins / Q000276:immunology / Q000302:isolation & purification*; D014733:Vibrio / Q000737:chemistry*; D014736:Vibrio parahaemolyticus / Q000737:chemistry
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; C073845:hemolysin, Vibrio; C068489:thermostable direct hemolysin