High-performance liquid chromatographic determination of ethacrynic acid in human plasma.
A high-performance liquid chromatographic method for the determination of ethacrynic acid (EA) in human plasma is described. Plasma was prepared for analysis by addition of 4-(2,4-dichlorophenoxy)-butyric acid as an internal standard followed by acidification with hydrochloric acid and extraction with ethyl acetate. Separation was by isocratic reversed-phase chromatography, the column effluent was monitored at 280 nm and quantitation was performed using peak-area ratios. The linear range for EA determination was from 0.5 to 25 micrograms/ml with a lower limit of detection of 0.1 microgram/ml. The reported method is convenient, sensitive and reproducible, illustrating its usefulness for pharmacokinetic studies.
LaCreta
F P
FP
Department of Pharmacology, Fox Chase Cancer Center, Philadelphia, PA 19111.
Brennan
J M
JM
Tinsley
P W
PW
O'Dwyer
P J
PJ
D000085:Acetates; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004976:Ethacrynic Acid / Q000097:blood*; D006801:Humans; D006851:Hydrochloric Acid
D000085:Acetates; C007650:ethyl acetate; D004976:Ethacrynic Acid; D006851:Hydrochloric Acid