A technique for cardiac denervation in Megalobulimus sanctipauli (Mollusca, Gastropoda, Pulmonata).
Cardiac nerve of Megalobulimus sanctipauli can be accessed by making an incision of about 1 cm with the aid of a scalpel introduced into the connective tissue (whitish scar) joining a fold of the anterior lobe of the digestive gland. The existence of an externally identifiable anatomic characteristic (whitish scar) facilities access to the nerve thus preventing large hemolymph loss. The percentage of hits of the technique was evaluated in two different groups (N = 14 and 17) of animals, with positive results obtained for 86% and 88%, respectively. The survival rate determined 13 days after surgery for one experimental group (N = 14) was 71%.
Romero
S M
SM
Departamento de Biologia, Faculdade de Filosofia, Universidade de São Paulo, Ribeirão Preto, Brasil.
Hoffmann
A
A
D000818:Animals; D006321:Heart / Q000294:innervation*; D008974:Mollusca; D009121:Muscle Denervation / Q000379:methods*