Application of industrial protease "Alcalase" in peptide synthesis.
An industrial alkaline protease "Alcalase" has been found to be very stable in organic solvents and usable as a catalyst for i) resolution of N-protected amino acids, in both aqueous solution and organic solvent with high yield and optical purity, ii) saponification of Fmoc-peptide esters in high concentration organic solvent iii) kinetically controlled peptide bond formation in ethanol solution, iv) diastereoselective hydrolysis of peptide esters, to prepare optically pure peptides.
Chen
S T
ST
Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
Chen
S Y
SY
Hsiao
S C
SC
Wang
K T
KT
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D004795:Enzyme Stability; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D012997:Solvents; D013237:Stereoisomerism; D013381:Subtilisins / Q000737:chemistry*
D000596:Amino Acids; D009842:Oligopeptides; D010455:Peptides; D012997:Solvents; D013381:Subtilisins