title: Effects of irradiation on marrow stromal cells with respect to committed granulocyte-macrophage progenitor cells.
abstract: The effects of irradiation on the growth regulatory function of marrow stromal cells (MSC), and on the committed granulocyte-macrophage progenitor cells (GM-CFC), were investigated using a liquid culture system. CSF activity in the supernatant of irradiated MSC (0-900 rads) increased markedly with the increase of MSC irradiation dose. CSF-inhibitory activity in the supernatant of irradiated MSC (0-900 rads) also increased with the increase of MSC irradiation dose. Furthermore, the activity of exogenous CSF added to the supernatant of 900 rad-irradiated MSC was lost more slowly than that of non-irradiated MSC. These data suggest that irradiation affected CSF production, inhibitor production and consumption of CSF by MSC.
authors.affiliation: First Department of Internal Medicine, Faculty of Medicine, Nihon University.
authors.forename: I
authors.initials: I
authors.lastname: Tsuboi
authors.forename: K
authors.initials: K
authors.lastname: Morimoto
authors.forename: A
authors.initials: A
authors.lastname: Sakuma
authors.forename: T
authors.initials: T
authors.lastname: Horie
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects*; D001854:Bone Marrow Cells; D006098:Granulocytes / Q000528:radiation effects*; D006412:Hematopoietic Stem Cells / Q000528:radiation effects*; D066298:In Vitro Techniques; D008264:Macrophages / Q000528:radiation effects*; D008297:Male; D051379:Mice