title: The effect of plasma solutes on total-body-water measurements via NMR.
abstract: Nuclear magnetic resonance (NMR) spectroscopy has been reported as an alternative method for quantitating deuterium oxide concentrations in the evaluation of total-body-water in humans. However, the presence of dissolved plasma proteins results in an underestimation of deuterium NMR (2H-NMR) intensity ratios, thereby causing an overestimation (5-6%) of total-body-water (TBW) values determined from nonsublimed patient plasma samples. We demonstrate that plasma samples must be corrected for the volume percentage of water in plasma. Correction of initial 2H-NMR intensity ratios with a factor of 0.93 results in intensity ratios comparable to those determined from plasma samples subjected to vacuum sublimation to remove all plasma solutes.
authors.affiliation: Department of Research, Baptist Medical Center, Birmingham, Alabama 35211.
authors.forename: E W
authors.initials: EW
authors.lastname: Richards
authors.forename: M
authors.initials: M
authors.lastname: Abu Khaled
authors.forename: C L
authors.initials: CL
authors.lastname: Watkins
authors.forename: C L
authors.initials: CL
authors.lastname: Long
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000032:analysis; D001823:Body Composition; D001834:Body Water; D003903:Deuterium; D005188:False Negative Reactions; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010949:Plasma / Q000737:chemistry*
chemical_list: D001798:Blood Proteins; D003903:Deuterium