[The excitatory effect of adenosine on the discharge activity of the afferent cardiac sympathetic fibers].
Recent clinical observations suggest that adenosine may produce cardiac pain; even though the mechanisms involved still need defining, one of the most convincing hypotheses seems to be a direct adenosine stimulation of the sympathetic nerve endings present in the myocardium. In 10 decerebrate and artificially ventilated cats, single sympathetic afferent fibres innervating the left ventricle were isolated from the third white thoracic ramus communicans of the left sympathetic chain. After locations of the fibre receptor field on the cardiac surface, we evaluated the effects of the local epicardial application of adenosine (0.1, 1 and 10 mg/ml) on the nervous discharge activity. These results were compared with those obtained by application of bradykinin, a potent natural algogenic substance which activates sympathetic afferents, and by a mechanical stimulus such as a slight increase of systolic arterial pressure (46 +/- 6% from 113 +/- 18 mmHg) induced by partial occlusion of the thoracic aorta. In particular, adenosine (1 mg/ml) elicited a significant increase in impulse activity (from 0.11 +/- 0.02 to 0.36 +/- 0.06 imp/0.1 s) with a latency of 16 +/- 2 s. Bradykinin application (20 micrograms/ml), in the same way, produced a significant increase in impulse activity (from 0.11 +/- 0.01 to 0.86 +/- 0.16 imp/0.1 s) with a latency of 8 +/- 1 s. Neither situation showed significant hemodynamic changes. An increased neural discharge (from 0.11 +/- 0.02 to 0.26 +/- 0.04 imp/0.1 s) was also observed during aortic occlusion. After purinergic receptor blockade by aminophylline (5 mg/kg, iv), the response to adenosine was no longer observed, while responses to bradykinin and aortic occlusion were unchanged.(ABSTRACT TRUNCATED AT 250 WORDS)
Montano
N
N
Istituto Ricerche Cardiovascolari, Università degli Studi, Milano.
Lombardi
F
F
Ruscone
T G
TG
Contini
M
M
Guazzi
M
M
Malliani
A
A
D000241:Adenosine / Q000494:pharmacology*; D000344:Afferent Pathways / Q000187:drug effects / Q000502:physiology; D000628:Aminophylline / Q000494:pharmacology; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D002415:Cats; D003655:Decerebrate State / Q000503:physiopathology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006321:Heart / Q000294:innervation* / Q000502:physiology; D009412:Nerve Fibers / Q000187:drug effects* / Q000502:physiology; D013268:Stimulation, Chemical; D013564:Sympathetic Nervous System / Q000187:drug effects* / Q000502:physiology; D013599:Systole / Q000187:drug effects / Q000502:physiology
D000628:Aminophylline; D000241:Adenosine; D001920:Bradykinin
Effetto eccitatorio dell'adenosina sull'attività di scarica delle fibre afferenti simpatiche cardiache.