Rapid determination of 5-hydroxytryptamine in whole blood by liquid chromatography with fluorimetric detection.
A rapid and simple reversed-phase (using muBondapak C18 as the stationary phase) liquid chromatographic method with fluorimetric detection is described for the quantitation of 5-hydroxytryptamine in whole blood. The rapidity and simplicity of the method are explained by the absence of a pretreatment. 5-Fluoro-dl-tryptophan was used as internal standard. The mobile phase was 0.01 M phosphate buffer (pH 4.5) with 0.0025 M 1-heptanesulfonic acid and 20% methanol. The detection wavelength were 302 nm for excitation and 340 nm for emission. Analysis time was 10 min with retention times for 5-hydroxytryptamine of 9 min and for 5-fluoro-dl-tryptophan of 7 min. This method is proposed for biological exploration of psychiatric disorders involving 5-hydroxytryptamine and would be useful for tryptophan.
Traiffort
E
E
Unité de Pharmacologie Clinique, Centre Hospitalier Sainte-Anne, Paris, France.
Hubert
P
P
Tayeb
N
N
Aymard
N
N
D002853:Chromatography, Liquid / Q000379:methods*; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D012701:Serotonin / Q000097:blood*
D012701:Serotonin