Reversed-phase liquid chromatographic method for the simultaneous determination of the antimalarial drugs sulfadoxine, pyrimethamine, mefloquine and its major carboxylic metabolite in plasma.
A high-performance liquid chromatographic (HPLC) method for the simultaneous determination of sulfadoxine, pyrimethamine, mefloquine and the carboxylic metabolite of mefloquine in plasma is described. After the proteins have been precipitated with a combination of zinc sulphate and acetonitrile containing two internal standards, pyrimethamine and mefloquine are extracted as bases and sulfadoxine and the carboxylic metabolite of mefloquine as ion-pairs with tetrabutylammonium. The drugs are separated by HPLC on a 3 micron octadecylsilica column with ultraviolet detection at 229 nm. The method is simple and reliable and enables the simultaneous determination of the drugs in 600-microliter plasma samples with a sensitivity suitable for standard drug monitoring purposes.
Bergqvist
Y
Y
Department of Clinical Chemistry, Falun Central Hospital, Sweden.
Eckerbom
S
S
Larsson
H
H
Malekzadeh
M
M
D000962:Antimalarials / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015767:Mefloquine / Q000097:blood; D011739:Pyrimethamine / Q000097:blood; D013413:Sulfadoxine / Q000097:blood*
D000962:Antimalarials; D013413:Sulfadoxine; D015767:Mefloquine; D011739:Pyrimethamine