[The growth of preneoplastic hepatocytes in the spleen of recipient mice].
Hepatocytes and the fraction of non parenchymal cells enriched with oval cells were extracted from the preneoplastic mouse liver at the stage of hyperplastic node formation and implanted into the spleen. In 14-16 months after the transplantation, multiple islets of hepatocytes which replaced up to 25% of the spleen cut area, were found in 57% (4 of 7) and 22% (8 of 36) of recipients respectively. The hepatocytes formed 2-3-cell bulks or solid masses organized into multicellular trabecules, and expressed biliary capillary antigen, albumin and transferrin. In the inoculation of nonparenchymal cell fraction, the growth of hepatic tissue in the spleen depended on the magnitude of hepatocyte admixture to be undetectable in absence of hepatocytes in the donor suspension. The growth of hepatic tissue in spleen was observed following the injection of a small number (3 x 10(-4)-6 x 10(-5)) of live hepatocytes. This fact evidences an extremely high clonogenic potency of clonogenic potency of preneoplastic hepatocytes.
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D000818:Animals; D001388:Aziridines; D002273:Carcinogens; D002469:Cell Separation / Q000379:methods; D006498:Hepatectomy; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology*; D016031:Liver Transplantation / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000473:pathology* / Q000637:transplantation; D011230:Precancerous Conditions / Q000139:chemically induced / Q000473:pathology*; D013154:Spleen / Q000473:pathology*; D013997:Time Factors; D014185:Transplantation, Isogeneic
D001388:Aziridines; D002273:Carcinogens; C007526:dipin
Rost preneoplasticheskikh gepatotsitov v selezenke mysheĭ-retsipientov.