Studies on the enzymatic coupling of peptide segments on the solid support.
Four different supports: polyacrylamide (I), polyethylene glycol-polystyrene MP-PEG (II), Kel-F-g-styrene (III) and controlled pore glass CPG (IV-VI) were tested in order to determine the most suitable carrier for both chemical and enzymatic methods. As a model of enzymatic reaction for comparative study of supports, the hydrolysis of peptide methyl esters bound to insoluble polymer was examined. Peptide segment couplings by means of papain were attempted on the CPG-support which was found to be the most suitable for performing enzymatic reactions. The racemization-free assemblies of partially protected peptides on this support were achieved in moderate yields (31-54%).
Slomczynska
U
U
Departament de Quimica Organica, Facultat de Quimica, Universitat de Barcelona, Spain.
Albericio
F
F
Cardenas
F
F
Giralt
E
E
D000180:Acrylic Resins; D000595:Amino Acid Sequence; D004798:Enzymes / Q000737:chemistry*; D005069:Evaluation Studies as Topic; D005898:Glass; D008969:Molecular Sequence Data; D010206:Papain / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D011092:Polyethylene Glycols; D011137:Polystyrenes; D020058:Styrene; D013343:Styrenes; D000099166:Polyacrylamides
D000180:Acrylic Resins; D004798:Enzymes; D010206:Papain; D010455:Peptides; D011092:Polyethylene Glycols; D011137:Polystyrenes; D020058:Styrene; D013343:Styrenes; D000099166:Polyacrylamides