Evaluation of a rapid dot-ELISA as a field test for the diagnosis of cystic hydatid disease.
A rapid dot enzyme-linked immunosorbent assay (dot-ELISA) was developed as a field test for the diagnosis of cystic hydatid disease caused by Echinococcus granulosus. The 30 min test was based on the detection of antibodies to antigen B of hydatid fluid and was carried out using 50 microliters of whole blood in a field assessment in the Turkana region of north-west Kenya. Initial laboratory studies showed antigen B to be preferable to crude cyst fluid, with 94% sensitivity and 90.3% specificity for Echinococcus infections. The field test was rapid, inexpensive and simple to perform and is considered to be a useful back-up to ultrasound scanning.
Rogan
M T
MT
Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, UK.
Craig
P S
PS
Zeyhle
E
E
Romig
T
T
Lubano
G M
GM
Deshan
L
L
D000909:Antibodies, Helminth / Q000032:analysis*; D004443:Echinococcosis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007630:Kenya; D013997:Time Factors
D000909:Antibodies, Helminth