Production and preparation of hyperimmune bovine colostrum for passive immunotherapy of cryptosporidiosis.
Pregnant cows were immunized to produce hyperimmune bovine colostrum (HBC) by intramuscular injection or intramammary infusion (TI) followed by 3 successive TI boosters with Cryptosporidium parvum (Cp) oocyst antigen mixed with Freund's (F) or Ribi (R) adjuvant. Control cows received no Cp. Colostrum from all cows was skimmed of butterfat and tested for specific anti-Cp immunoglobulin isotypes by ELISA. The HBC from Cp-F and Cp-R immunized cows had IgG1 titers exceeding 1:400,000 and 1:800,000, respectively. Some HBC from Cp-F immunized cows was freeze-dried to facilitate storage and some were irradiated at 42.5 kGy to kill potentially contaminating pathogens. Freeze-drying, but not irradiation, reduced IgG1 titers by only one dilution. Neither treatment affected Western blot banding patterns.
Fayer
R
R
USDA, ARS, LPSI, Beltsville, MD 20705.
Tilley
M
M
Upton
S J
SJ
Guidry
A J
AJ
Thayer
D W
DW
Hildreth
M
M
Thomson
J
J
D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D003126:Colostrum / Q000276:immunology* / Q000382:microbiology / Q000528:radiation effects; D003457:Cryptosporidiosis / Q000628:therapy*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005612:Freeze Drying; D007132:Immunoglobulin Isotypes / Q000276:immunology; D016219:Immunotherapy, Adoptive; D014611:Vaccination
D007132:Immunoglobulin Isotypes