252Cf-plasma desorption and cesium-ion liquid secondary-ion mass spectrometric analysis of recombinant proteins.
The 35 keV Cs+ liquid secondary-ion mass spectrometry (LSIMS) and 252Cf-plasma desorption (PD) mass spectra of recombinant proteins in the 10-25 kDa mass range are compared. Both techniques showed comparable mass accuracy and sensitivity, and in the case of LSIMS, remarkably short analysis time. Analysis by the PD/nitrocellulose method demonstrated slightly higher sensitivity and relatively lower dependence on the salt and buffer content of the protein sample.
Tsarbopoulos
A
A
American Cyanamid Company, Lederle Laboratories, Pearl River, NY 10965.
Pramanik
B N
BN
Reichert
P
P
Siegel
M M
MM
Nagabhushan
T L
TL
Trotta
P P
PP
D000818:Animals; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000737:chemistry; D006801:Humans; D016898:Interferon-alpha / Q000737:chemistry; D015847:Interleukin-4 / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000737:chemistry*; D013552:Swine
D016898:Interferon-alpha; D011994:Recombinant Proteins; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor