Isolation of IgGT from hyperimmune horse anti-snake venom serum: its protective ability.
Hyperimmune horse anti-bothropic serum, used in serum therapy, was analyzed for its IgGT content and protective ability. IgGT was isolated through a combination of salt-mediated hydrophobic chromatography and protein A affinity chromatography. The chromatographic fractions obtained were analyzed with regard to their isotype content and protective ability. The results suggest that the protective ability of hyperimmune anti-venom serum is located mainly in the IgGT subclass.
Fernandes
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Immunology Research and Training Center Otto G. Bier, Instituto Butantan, São Paulo, Brazil.
Takehara
H A
HA
Mota
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D000818:Animals; D000997:Antivenins / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D003435:Crotalid Venoms / Q000276:immunology* / Q000633:toxicity; D006736:Horses / Q000276:immunology*; D007114:Immunization; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007132:Immunoglobulin Isotypes / Q000302:isolation & purification; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000276:immunology
D000997:Antivenins; D003435:Crotalid Venoms; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D013205:Staphylococcal Protein A