Enzymatic synthesis of novel oligosaccharides by use of transglycosylation activity of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae.
The transglycosylation activity of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae was used for the enzymatic synthesis of novel oligosaccharides. When (Man)6(GlcNAc)2Asn was used as a substrate for the transglycosylation, (Man)6GlcNAc-Glc, (Man)6GlcNAc-Man, (Man)6GlcNAc-chitobiose, and (Man)6GlcNAc-gentiobiose were synthesized. Their structures were identified by HPLC, ion spray mass spectrometry, and digestion with glycosidases. Endo-beta-N-acetylglucosaminidases hydrolyzed the pyridylamino derivatives of these oligosaccharides.
Takegawa
K
K
Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan.
Yamaguchi
S
S
Kondo
A
A
Kato
I
I
Iwahara
S
S
D001173:Arthrobacter / Q000201:enzymology*; D001426:Bacterial Proteins / Q000378:metabolism*; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000378:metabolism; D006031:Glycosylation; D006868:Hydrolysis; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / Q000378:metabolism*; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000378:metabolism; D009844:Oligosaccharides / Q000096:biosynthesis*
D001426:Bacterial Proteins; D004187:Disaccharides; D009005:Monosaccharides; D009844:Oligosaccharides; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase