Importance of Ca2+, K+ and glucose in the medium for sperm penetration through the human zona pellucida.
When salt-stored human oocytes were inseminated in the regular mBWW medium and examined 6 hr later, the zonae of almost all of the oocytes were penetrated by spermatozoa. In contrast, none of zonae were penetrated when the oocytes were inseminated in either Ca(2+)-free, K(+)-free or glucose-free medium. The failure of zona penetration by spermatozoa in Ca(2+)-free and K(+)-free media is likely to be due to the inhibition of the acrosome reaction. Spermatozoa were unable to exhibit hyperactivated motility in glucose-free medium, suggesting that the failure of zona penetration in this medium is due, at least in part, to the inhibition of hyperactivated motility of spermatozoa.
Hoshi
K
K
Department of Obstetrics and Gynecology, Fukushima Medical College.
Tsukikawa
S
S
Sato
A
A
D002118:Calcium / Q000494:pharmacology / Q000502:physiology*; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology; D005260:Female; D005947:Glucose / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008274:Magnesium / Q000502:physiology; D008297:Male; D009865:Oocytes / Q000187:drug effects; D011188:Potassium / Q000494:pharmacology / Q000502:physiology*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*; D015044:Zona Pellucida / Q000502:physiology*
D008274:Magnesium; D005947:Glucose; D011188:Potassium; D002118:Calcium