title: Efficient mutagenesis, expression and purification of procathepsin D.
authors.affiliation: Department of Medicine, University of Texas Health Science Center, San Antonio 78284-7877.
authors.forename: D
authors.initials: D
authors.lastname: Sachdev
authors.forename: J
authors.initials: J
authors.lastname: Schorey
authors.forename: J
authors.initials: J
authors.lastname: Chirgwin
mesh_terms: D002402:Cathepsin D / Q000235:genetics* / Q000302:isolation & purification; D002461:Cell Line, Transformed; D004705:Endocytosis; D004792:Enzyme Precursors / Q000235:genetics* / Q000302:isolation & purification; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D016296:Mutagenesis; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004792:Enzyme Precursors; D016271:Proto-Oncogene Proteins c-myc; D011993:Recombinant Fusion Proteins; C058345:procathepsin D; D002402:Cathepsin D