[Chronic effect of ammonium fluoride on selected parameters of microsomal fracture of the rat liver with special reference to the cytochrome P-450 system].
Male Wistar rats were exposed to NH4F in concentration corresponding to mean annual limit of fluoride compounds in the atmospheric air. After 3, 6 and 9 months a microsomal fraction was isolated from the liver, and the composition as well as the metabolic activity of this fraction was determined. The content of microsomal protein increased after 3-month-long period of experiment, and subsequently it dropped after the period of 9 months. The content of phospholipids decreased after 3 months. The content of microsomal cholesterol was particularly high after a 6-month-long experiment. There were also changes in the contents of individual phospholipid fractions, and fatty acids of phospholipids. The content of cytochrome P-450, cytochrome b5 and activity of NADH-cytochrome b5 reductase did not change. Activity of NADPH-dependent reductase of cytochrome c--decreased after the period of 9 months. Moreover, as consequence of changes in the activity of cytochrome P-450 system and the endoplasmic reticulum composition, alterations were observed in the metabolism of the tested substrates i.e. aniline and aminopyrine. The aniline turnover was inhibited after 6 and that of aminopyrine after 9 months experiment. The observed changes may prove that the detoxication capacity of the liver was impaired due to being exposed to ammonium fluoride.
Juzyszyn
Z
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Zakładu Toksykologii Instytutu Farmakologii i Toksykologii, Pomorskiej Akademii Medycznej.
D000393:Air Pollutants / Q000633:toxicity*; D064751:Ammonium Compounds; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005459:Fluorides / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000494:pharmacology / Q000633:toxicity; D008658:Inactivation, Metabolic / Q000502:physiology; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D008954:Models, Biological; D009245:NADH Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D010743:Phospholipids / Q000037:antagonists & inhibitors / Q000378:metabolism; D000644:Quaternary Ammonium Compounds; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000393:Air Pollutants; D064751:Ammonium Compounds; D010743:Phospholipids; D000644:Quaternary Ammonium Compounds; C024822:ammonium fluoride; D003577:Cytochrome P-450 Enzyme System; D009245:NADH Dehydrogenase; D005459:Fluorides
Przewlekły wpływ fluorku amonowego na wybrane parametry frakcji mikrosomalnej watroby szczura ze szczególnym uwzglednieniem układu cytochromu P-450.