title: The value of enzyme-linked immunosorbent assay (ELISA) for diagnosis of human trichinosis.
abstract: Sera were collected from proven cases of trichinosis and other helminthic infections and examined for IgG and IgM antibodies by an enzyme-linked immunosorbent assay (ELISA). IgG-ELISA was positive in 68.75, 100, and 100% of cases on 23rd, 50th, 57th day of infection while IgM-ELISA was positive in 12.50, 85.71, 93.33% respectively. In the group with parasitic infections other than trichinosis 20.93% were positive by IgG-ELISA and strong cross-reactivity was prominent with intestinal capillariasis. With IgM-ELISA, positivity in the group with other parasitic infections was 9.30%. It was suggested that sera should be examined not before 1 month after infection to achieve maximum sensitivity for serodiagnosis of human trichinosis.
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Thailand.
authors.forename: N
authors.initials: N
authors.lastname: Morakote
authors.forename: C
authors.initials: C
authors.lastname: Khamboonruang
authors.forename: V
authors.initials: V
authors.lastname: Siriprasert
authors.forename: S
authors.initials: S
authors.lastname: Suphawitayanukul
authors.forename: S
authors.initials: S
authors.lastname: Marcanantachoti
authors.forename: W
authors.initials: W
authors.lastname: Thamasonthi
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000175:diagnosis*
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D007075:Immunoglobulin M