Fibrinolytic enzyme from Agkistrodon halys brevicaudus (Korean mamushi) snake venom.
By means of DEAE-Sepharose CL-6B ion exchange chromatography and TSK-GEL G2000 SW high-performance gel filtration, a purified protein with fibrinolytic activity was obtained from the venom of Agkistrodon halys brevicaudus (Korean mamushi). The protein was homogeneous as judged by isoelectric focusing electrophoresis and high-performance gel filtration. Its mol. wt is 39,200 and its isoelectric point 4.12. The specific fibrinolytic activity of the protein was 3.2 times higher than that of the crude venom. The fibrinolytic activity of the purified principle was 33 units/mg protein (units of standard urokinase activity).
Qian
X H
XH
Institute of Radiation Medicine, Academy of Medicine Science, Beijing, People's Republic of China.
Ma
L
L
D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003435:Crotalid Venoms / Q000737:chemistry / Q000201:enzymology* / Q000494:pharmacology; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D007525:Isoelectric Focusing; D008970:Molecular Weight; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis
C031442:Agkistrodon venoms; D000596:Amino Acids; D003435:Crotalid Venoms; D005343:Fibrinolytic Agents; D014568:Urokinase-Type Plasminogen Activator