Fibroblast and endothelial outgrowth from human Tenon's explants: inhibition of fibroblast growth by 5HT receptor antagonism.
Tenon's layer capsule is a major source of fibroplasia associated with post-traumatic wound healing in the external eye and failure of filtration surgery for glaucoma (trabeculectomy). We have investigated the cell types involved in outgrowth from human Tenon's layer explants in culture. Outgrowth from explants maintained in fetal bovine serum (5%) and fibroblast conditioned medium (25%) consisted of endothelial cells exhibiting factor VIII antibody staining and were located to sectioned capillary ends. In media supplemented with human serum (10%), fibroblast outgrowth was observed from the entire perimeter of explants. The mitogenic stimulus of human serum on fibroblasts, monitored by 3H-thymidine uptake, was dose-dependent and accompanied by a 200% increase in inositol phosphate production. 5HT induced a significant increase in fibroblast proliferation and 5HT receptor antagonism with methysergide (20 microM) reduced serum-induced mitogenesis by 30%. These findings support the role of fibroblasts in failure of filtration surgery for glaucoma and suggest a role for 5HT in serum-derived Tenon's fibroplasia.
Mullaney
P
P
Department of Ophthalmology, Royal Victoria Eye and Ear Hospital, Dublin, Ireland.
Curren
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Collum
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Kilfeather
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D001769:Blood; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D004727:Endothelium / Q000166:cytology* / Q000187:drug effects; D005123:Eye / Q000166:cytology* / Q000187:drug effects; D005205:Fascia / Q000166:cytology / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D008784:Methysergide / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology*; D012702:Serotonin Antagonists; D013936:Thymidine / Q000378:metabolism; D014130:Trabeculectomy
D003470:Culture Media; D007295:Inositol Phosphates; D012702:Serotonin Antagonists; D012701:Serotonin; D013936:Thymidine; D008784:Methysergide