title: Tear volume in early and late phases of ocular anaphylaxis in guinea pigs.
abstract: Three models of ocular anaphylaxis containing 6 guinea pigs each were used to examine their effect on tear volume. In the first model, IgG1-rich anti-dinitrophenol (DNP) bovine gamma-globulin serum was injected subconjunctivally, and the animals were challenged 4 h later with topically applied di-DNP-lysine. In the second model, IgE-rich anti-egg albumin serum was injected subconjunctivally, and the animals were challenged 8 days later with topical instillation of egg albumin. In the third model, IgE-rich anti-egg albumin serum was injected intravenously, and animals were challenged 8 days later with topically applied egg albumin. Tears were collected before the procedure, 0.5 h after anaphylaxis was initiated, and then hourly for 8, 10, 12, 24, and 48 h after challenge. All animal eyes undergoing ocular anaphylaxis had significantly increased tearing. Tear volumes remained elevated in the first model beyond the observation period of 48 h.
authors.affiliation: Eye Research Institute, Boston, Mass.
authors.forename: T
authors.initials: T
authors.lastname: Saiga
authors.forename: M R
authors.initials: MR
authors.lastname: Allansmith
mesh_terms: D000707:Anaphylaxis / Q000503:physiopathology*; D000818:Animals; D004136:Dinitrobenzenes; D004195:Disease Models, Animal; D005128:Eye Diseases / Q000503:physiopathology*; D005260:Female; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000503:physiopathology; D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; D013666:Tears / Q000378:metabolism*
chemical_list: C045618:2,4-dinitrophenyl-IgG; D004136:Dinitrobenzenes; D007074:Immunoglobulin G; D007166:Immunosuppressive Agents