title: Immunopurification of an S-antigen-like protein from human platelets.
abstract: S-antigen (also named arrestin or 48K protein) is a protein abundant in photoreceptor cells of vertebrates and invertebrates. The presently known function of this protein in retina is to arrest the enzymatic cascade of phototransduction in retinal rods, through its binding to photoactivated and phosphorylated rhodopsin. Proteins closely related to S-antigen were recently demonstrated in several non photosensitive cells. In this work, we demonstrated the presence of a protein similar to retinal S-antigen with regards to its immunoreactivity with a panel of monoclonal antibodies and its molecular weight in soluble extracts of human platelets. This protein was purified by immunoaffinity chromatography using a rabbit antibody to retinal S-antigen. This S-antigen-like protein could have a regulatory function in G-protein-mediated transduction of chemical signals in platelets, similar to arrestin function in phototransduction.
authors.affiliation: INSERM U 86, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Mirshahi
authors.forename: A
authors.initials: A
authors.lastname: Razaghi
authors.forename: S S
authors.initials: SS
authors.lastname: Mirshahi
authors.forename: V
authors.initials: V
authors.lastname: Van Tuyen
authors.forename: J P
authors.initials: JP
authors.lastname: Faure
mesh_terms: D000911:Antibodies, Monoclonal; D000941:Antigens / Q000737:chemistry / Q000302:isolation & purification*; D019393:Arrestin; D001792:Blood Platelets / Q000737:chemistry*; D015153:Blotting, Western; D005136:Eye Proteins / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D007163:Immunosorbent Techniques; D008970:Molecular Weight; D015398:Signal Transduction
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D019393:Arrestin; D005136:Eye Proteins