[Toxicity of 4-chloro-2-nitroaniline and 2-chloro-4-nitroaniline to isolated rat hepatocytes].
The toxicity of 4-chloro-2-nitroaniline (4C2NA) and 2-chloro-4-nitroaniline (2C4NA) was investigated on isolated rat hepatocytes following 1-3 hours of exposure to 0.2 and/or 2 mM of these xenobiotics. The higher of the two concentrations appeared to induce a statistically significant loss of cellular viability (p less than 0.01 compared to control), judged by Trypan Blue staining, after 3 hours of incubation with these substances means = 58, SD = 7%; and means = SD = 7%; for 4C2NA and 2C4NA, respectively). Furthermore, both chloronitroanilines produced an hepatocellular and microsomal damage demonstrated by conspicuous changes in LDH and G-6-Pase activities (p less than 0.01). The exposure to 2 mM of both 4C2NA and/or 2C4NA produced a marked depletion of the intracellular pool of GSH after 3 hours (13 mM/10(6) and 10 mM/10(6) cells, respectively; p less than 0.01). Thus it seems possible that 2C4NA may induce a more severe cellular damage than that induced by 4C2NA.
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Istituto di Patologia Generale, Università di Genova.
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Domenicotti
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D000814:Aniline Compounds / Q000633:toxicity*; D000818:Animals; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014343:Trypan Blue
D000814:Aniline Compounds; C071396:2-chloro-4-nitroaniline; C040199:4-chloro-2-nitroaniline; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D014343:Trypan Blue; D004121:Dimethyl Sulfoxide
Tossicità della 4-cloro-2-nitroanilina e della 2-cloro-4-nitroanilina su epatociti isolati di ratto.