Evidence for secretion of 7B2 by A- and B-cells of hamster pancreatic islets.
7B2 is a neuroendocrine protein, and in the pancreatic islets the presence of 7B2 in A- and B-cells was immunohistochemically demonstrated. In order to examine 7B2 secretion by A- and B-cells of pancreatic islets, we prepared isolated hamster pancreatic islet cells as well as an A-cell-rich culture, and studied 7B2 secretion under certain stimulations. 7B2 was secreted by isolated hamster pancreatic islet cells. This secretion was stimulated by theophylline and arginine, but glucose had a weak effect on the 7B2 secretion. Such a response of 7B2 to the stimulations was different from that of insulin or glucagon. 7B2 secretion was also noted in the A-cell-rich culture. These results suggest that 7B2 is secreted by both A- and B-cells of the hamster pancreatic islets and its secretion is regulated under certain conditions.
Iguchi
H
H
Department of Biochemistry, National Kyushu Cancer Center, Fukuoka, Japan.
Okeda
T
T
Takaki
R
R
D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002478:Cells, Cultured; D002850:Chromatography, Gel; D006224:Cricetinae; D005934:Glucagon / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007150:Immunohistochemistry; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism*; D008647:Mesocricetus; D009419:Nerve Tissue Proteins; D051859:Neuroendocrine Secretory Protein 7B2; D010907:Pituitary Hormones / Q000378:metabolism*; D013806:Theophylline / Q000494:pharmacology
D007328:Insulin; D009419:Nerve Tissue Proteins; D051859:Neuroendocrine Secretory Protein 7B2; D010907:Pituitary Hormones; D005934:Glucagon; D001120:Arginine; D013806:Theophylline; D005947:Glucose