title: Augmentation by interleukins of the antibody response to a conjugate vaccine against Haemophilus influenzae b.
abstract: Interleukins have been recognized as potential adjuvants for use during vaccination. The immunogenicity of some poorly immunogenic bacterial capsular polysaccharides have been improved by conjugation to a protein carrier. Augmentation of the immune response to these glycoconjugates, however, may be realized in the presence of interleukins. The antibody response to one such vaccine which comprises a oligosaccharide derived from the capsule of Haemophilus influenzae type b coupled to CRM197 (HbOC) can be augmented in this manner. A suboptimal dose (0.1 microgram) of HbOC and varying concentrations of IL-1 alpha or IL-1 beta (10(2) - 5 x 10(5) U) were injected intramuscularly at 0 and 2 weeks into Swiss Webster mice. Vaccines were also formulated with and without aluminum phosphate. Antibody to the oligosaccharide was determined by Farr assay. In 3/3 experiments, IL-1 alpha enhanced primary and secondary antibody responses whereas with IL-1 beta, only a slight increase in the primary antibody response was seen but enhanced secondary responses were observed. Thus, IL-1 alpha and to some extent IL-1 beta enhanced the primary and secondary antibody responses to a glycoconjugate vaccine.
authors.affiliation: Praxis Biologics, Inc., Rochester, New York 14623.
authors.forename: G S
authors.initials: GS
authors.lastname: Bixler
authors.forename: S
authors.initials: S
authors.lastname: Pillai
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D006001:Glycoconjugates / Q000276:immunology; D006193:Haemophilus influenzae / Q000276:immunology*; D007375:Interleukin-1 / Q000008:administration & dosage*; D051379:Mice
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D006001:Glycoconjugates; D007375:Interleukin-1