Comparison of methods for HIV isolation from monocytes and lymphocytes of seropositive asymptomatic hemophiliacs and homosexuals.
1. Since monocyte-macrophages have been recognized as HIV targets in addition to CD4+ T-lymphocytes, we have evaluated HIV infection of purified peripheral blood mononuclear cell fractions obtained from 10 seropositive asymptomatic hemophiliacs and compared it with that of 10 asymptomatic homosexual patients. 2. HIV was isolated more frequently from the lymphocytes than the monocytes of both groups of patients. 3. HIV preferentially replicated in phytohemagglutinin-stimulated lymphocytes compared with growth factor-treated monocytes. Monocytes did not preferentially harbour HIV in either group.
Lemos
R S
RS
Department of Medicine, Tulane University School of Medicine, New Orleans 70112.
Rangan
S R
SR
Andes
W A
WA
Hyslop
N E
NE
D001782:Blood Donors; D006679:HIV Seropositivity / Q000382:microbiology*; D015497:HIV-1 / Q000302:isolation & purification* / Q000502:physiology; D006467:Hemophilia A / Q000382:microbiology*; D006716:Homosexuality; D006801:Humans; D009000:Monocytes / Q000382:microbiology*; D013601:T-Lymphocytes / Q000382:microbiology*; D014779:Virus Replication