title: Two-dimensional mapping by high-performance liquid chromatography of pyridylamino oligosaccharides from various glycosphingolipids.
abstract: A method to map sugars two-dimensionally for the analysis of the structures of oligosaccharides from glycosphingolipids is described. Nine neutral and ten acidic oligosaccharides were obtained from glycosphingolipids by endoglycoceramidase digestion and labelled with 2-aminopyridine. The pyridylamino oligosaccharides were clearly separated by high-performance liquid chromatography on commercially available C18-silica and amide-silica column. All compounds tested were mapped without any overlapping. The separation of the pyridylamino oligosaccharides on the C18-silica column depended on the numbers and positions of sialic acid and N-acetylhexosamine residues; on the amide-silica column, the separation depended on the total number of sugar residues.
authors.affiliation: Biotechnology Research Laboratories, Takuro Shuzo Co., Ltd., Shiga, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ohara
authors.forename: M
authors.initials: M
authors.lastname: Sano
authors.forename: A
authors.initials: A
authors.lastname: Kondo
authors.forename: I
authors.initials: I
authors.lastname: Kato
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006028:Glycosphingolipids / Q000032:analysis*; D006595:Hexosamines / Q000032:analysis; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D012794:Sialic Acids / Q000032:analysis
chemical_list: D006028:Glycosphingolipids; D006595:Hexosamines; D009844:Oligosaccharides; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid