Antitumoral effects of Pseudomonas aeruginosa lectins on Lewis lung carcinoma cells cultured in vitro without and with murine splenocytes.
Examination of the in vitro effects of PA-I and PA-II lectins of Pseudomonas aeruginosa on Lewis lung carcinoma cells revealed that these lectins differ in their effects. PA-I, the galactophilic lectin, exhibited both cytotoxic and cytostatic activities on these cells (tested by [3H]thymidine incorporation and by crystal violet vital staining). The two activities were dose and time dependent and inhibitable by the addition of methyl-alpha-D-galactoside to the culture medium. PA-II, the L-fucose and D-mannose binding lectin of the same Pseudomonas strain did not exhibit such a direct toxic effect on the tumor cells but affected them in the presence of splenocytes. Its addition to the tumor cells cocultured with murine (C57B1) splenocytes led to a profound cytolysis of the tumor cells, an effect which was inhibited by L-fucose.
Avichezer
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D
Department of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
Gilboa-Garber
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N
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002277:Carcinoma / Q000188:drug therapy*; D002470:Cell Survival / Q000187:drug effects; D005643:Fucose / Q000378:metabolism; D005840:Gentian Violet; D037102:Lectins; D008175:Lung Neoplasms / Q000188:drug therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D013154:Spleen / Q000166:cytology* / Q000187:drug effects; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D037102:Lectins; D005643:Fucose; D005840:Gentian Violet; D013936:Thymidine