[The effect of a conditioned medium from neonatal rat hepatocytes on a mixed culture of Kupffer cells and fibroblast-like liver cells].
The study was carried out on primary coculture Kupffer cells and liver fibroblasts of newborn and mature rats. At first Kupffer cells and liver fibroblasts were taken on the equal quantity. There were observed significant decrease of Kupffer cells quantity and fibroblasts death after 4-5 days in coculture. Mitotic and functional activity liver fibroblasts, adhesion of Kupffer cells were increased by the using of conditioned medium of newborn rat hepatocytes. The rise of mitotic activity of liver fibroblasts and quantity of nucleolus in their nuclei forestalls of the increase Kupffer cells size, appearing of Kupffer cells with some nuclei.
Stvolinskaia
N S
NS
Tabuntsova
S I
SI
Korovkin
B F
BF
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D007728:Kupffer Cells / Q000166:cytology / Q000187:drug effects*; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008938:Mitosis / Q000187:drug effects; D051381:Rats; D013997:Time Factors
D003470:Culture Media
Vliianie konditsionirovannoĭ sredy gepatotsitov novorozhdennykh krys na smeshannuiu kul'turu kupferovskikh i fibroblastopodobnykh kletok pecheni.