title: Isolation and structure of cytostatic steroidal saponins from the African medicinal plant Balanites aegyptica.
abstract: Bioactivity-guided separation of a CH2Cl2/MeOH extract of Balanites aegyptica afforded four new cytostatic saponins, named balanitins 4 [1], 5 [2], 6 [3], and 7 [4]. On the basis of enzymatic hydrolyses and glycosidation nmr chemical shifts employing the peracetates, structures 1-4 were established as yamogenin 3 beta-O-beta-D-glucopyranosyl-(1----3)-beta-D-glucopyranosyl-(1----4)-[al pha- L-rhamnopyranosyl-(1----2)]-beta-D-glucopyranoside [1], yamogenin 3 beta-O-alpha-L-rhamnopyranosyl-(1----3)-beta-D-glucopyranosyl-(1----4)- [alpha-L-rhamnopyranosyl-(1----2)]-beta-D-glucopyranoside [2], yamogenin 3 beta-O-beta-D-glucopyranosyl-(1----4)-[alpha-L- rhamnopyranosyl-(1----2)]-beta-D-glucopyranoside [3], and diosgenin 3 beta-O-beta-D-xylopyranosyl-(1----3)-beta-D-glucopyranosyl-(1----4)-[alp ha- L-rhamnopyranosyl-(1----2)]-beta-D-glucopyranoside [4].
authors.affiliation: Cancer Research Institute, Arizona State University, Tempe 85287.
authors.forename: G R
authors.initials: GR
authors.lastname: Pettit
authors.forename: D L
authors.initials: DL
authors.lastname: Doubek
authors.forename: D L
authors.initials: DL
authors.lastname: Herald
authors.forename: A
authors.initials: A
authors.lastname: Numata
authors.forename: C
authors.initials: C
authors.lastname: Takahasi
authors.forename: R
authors.initials: R
authors.lastname: Fujiki
authors.forename: T
authors.initials: T
authors.lastname: Miyamoto
mesh_terms: D000349:Africa; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002240:Carbohydrate Sequence; D007941:Leukemia P388 / Q000188:drug therapy; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D012503:Saponins