title: Radioimmunodetection of human colon cancer in nude mice by a new monoclonal antibody A7 against human colorectal cancer.
abstract: The in vivo localization of a monoclonal antibody A7 against a human colorectal cancer was studied in nude mice bearing human solid carcinomas, to evaluate potential applications of this antibody for radioimmunodetection of cancer. The tissue distribution of 125I-labeled A7 MoAb at 3 days after i.v. injection into mice bearing five different kinds of human solid tumors revealed a high uptake ratio by colon cancer, mammary cancer, and glioblastoma. In contrast, the uptake ratio by murine colorectal cancer (Colon-38) was extremely low. In immunoscintigraphic studies, HCT-15, one of the human colon cancer, was clearly visualized with 111In-DTPA-A7 MoAb. Glioblastoma was also imaged with the same extent. These results suggest that A7 MoAb would be applicable to the in vivo radioimmunodetection of colon- and mammary-cancer, and of glioblastoma.
authors.affiliation: Research Institute for Biosciences, Science University of Tokyo, Chiba, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kojima
authors.forename: N
authors.initials: N
authors.lastname: Shimura
authors.forename: A
authors.initials: A
authors.lastname: Kubodera
authors.forename: K
authors.initials: K
authors.lastname: Kubota
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Oyamada
mesh_terms: D000230:Adenocarcinoma / Q000000981:diagnostic imaging; D000818:Animals; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging*; D015179:Colorectal Neoplasms / Q000276:immunology*; D005260:Female; D005729:Ganglioneuroma / Q000000981:diagnostic imaging; D006801:Humans; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016719:Radioimmunodetection; D014018:Tissue Distribution
chemical_list: D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes