title: [Polysaccharides from Nerium oleander: structure and biological activity].
abstract: A water extraction of crushed leaves of Nerium oleander yielded 2.3% of a crude polysaccharide. The main fraction (67%) represents a pectic polysaccharide mainly composed of galacturonic acid besides rhamnose, arabinose and galactose. The polysaccharide structure was characterized by NMR, mild acid- and pectinase treatment combined with GC-MS analyses. In vivo tests for a possible antitumor activity did not result in a significant action. Investigation of immunomodulating activity brought some indications for mitogenic activity and a weak macrophage-mediated cytotoxicity. Increase of phagocytosis could not be doubtlessly assigned to the polysaccharide due to the high amount of endotoxin in the homogenous fraction OLE 2.
authors.affiliation: Lehrstuhl für Pharmazeutische Biologie, Universität Regensburg.
authors.forename: B M
authors.initials: BM
authors.lastname: Müller
authors.forename: F
authors.initials: F
authors.lastname: Rosskopf
authors.forename: D H
authors.initials: DH
authors.lastname: Paper
authors.forename: J
authors.initials: J
authors.lastname: Kraus
authors.forename: G
authors.initials: G
authors.lastname: Franz
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006868:Hydrolysis; D007155:Immunologic Factors / Q000494:pharmacology; D066298:In Vitro Techniques; D008033:Limulus Test; D008214:Lymphocytes / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008745:Methylation; D051379:Mice; D008809:Mice, Inbred C3H; D008934:Mitogens / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D007155:Immunologic Factors; D008934:Mitogens; D010936:Plant Extracts; D011134:Polysaccharides; D011480:Protease Inhibitors
vernacular_title: Polysaccharide aus Nerium oleander: Struktur und biologische Aktivität.