Regio- and stereoselective enzymatic esterification of glycerol and its derivatives.
A methodology for regio- and stereoselective preparation of acyl glycerol derivatives is presented. It offers easy access to specific 1,2-, 1,3-diglycerides and triglycerides as well as alkyl glycerol esters, phospholipids and glycolipids. These compounds are prepared by esterification of the corresponding glycerol derivatives such as 2-monoglycerides, alkyl glycerols, glyceryl glycosides, glyceryl phosphate esters, or unsubstituted glycerol. The regio- and stereoselectivity in the esterification is achieved by using fatty acid anhydrides and an enzymatic catalyst, 1,3-specific lipase. NMR methods for determining the regio- and stereoselectivity of esterification are discussed.
Mazur
A W
AW
Procter and Gamble Co., Miami Valley Laboratories, Cincinnati, OH 45239-8707.
Hiler
G D
GD
Lee
S S
SS
Armstrong
M P
MP
Wendel
J D
JD
D004951:Esterification; D005990:Glycerol / Q000031:analogs & derivatives / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D013237:Stereoisomerism; D013379:Substrate Specificity
D008049:Lipase; D005990:Glycerol