Aldehyde dehydrogenase (EC 1.2.1.3): comparison of subcellular localization of the third isozyme that dehydrogenates gamma-aminobutyraldehyde in rat, guinea pig and human liver.
1. Subcellular fractionation of rat, guinea pig and human livers showed that aldehyde dehydrogenase metabolizing gamma-aminobutyraldehyde was exclusively localized in the cytoplasmic fraction in all three mammalian species. 2. Total gamma-aminobutyraldehyde activity of aldehyde dehydrogenase was found to be ca 0.41, 0.3 and 0.24 mumol NADH min-1 g-1 tissue, respectively in rat, guinea pig and human liver, with more than 95% of activity in the cytoplasm. 3. Partially purified cytoplasmic isozyme from rat liver showed similar chromatographic behavior and kinetic properties to the E3 isozyme isolated from human liver. 4. The rat isozyme was insensitive to disulfiram (40 microM) and to magnesium (160 microM) and had Km values of 5 microM (pH 7.4) for gamma-aminobutyraldehyde, 7.5 microM (pH 9.0) for propionaldehyde and 4 microM (pH 7.4) for NAD.
Ambroziak
W
W
Center of Alcohol Studies, Rutgers University, Piscataway, NJ 08855-0969.
Kurys
G
G
Pietruszko
R
R
D000444:Aldehyde Dehydrogenase / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D002845:Chromatography; D003593:Cytoplasm / Q000201:enzymology; D004221:Disulfiram / Q000494:pharmacology; D005260:Female; D005969:Glutamate Dehydrogenase / Q000032:analysis / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000032:analysis / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D051381:Rats; D011919:Rats, Inbred Strains
D000447:Aldehydes; D007527:Isoenzymes; C006509:4-aminobutyraldehyde; D007770:L-Lactate Dehydrogenase; D000444:Aldehyde Dehydrogenase; D005969:Glutamate Dehydrogenase; D008274:Magnesium; D004221:Disulfiram