title: Antisense DNA downregulates protein kinase C isozymes (beta and alpha) and insulin-stimulated 2-deoxyglucose uptake in rat adipocytes.
abstract: Rat adipocytes were treated with antisense dimethoxytrityl pentadecadeoxynucleotides, complementary to mRNA initiation codon regions for alpha and beta isozymes of protein kinase C (PKC). This antisense treatment provoked 50-70% decreases in PKC and insulin-stimulated 2-deoxyglucose uptake, but did not inhibit insulin-stimulated diacylglycerol synthesis. Sense or nonsense oligodeoxynucleotides were without effect on PKC and 2-deoxyglucose uptake. These results suggest that: (i) PKC-alpha and PKC-beta isozymes can be specifically downregulated in rat adipocytes by antisense oligodeoxynucleotides, and (ii) insulin-stimulated glucose transport requires PKC.
authors.affiliation: James A. Haley Veterans' Hospital, Department of Internal Medicine, Tampa, FL.
authors.forename: R V
authors.initials: RV
authors.lastname: Farese
authors.forename: M L
authors.initials: ML
authors.lastname: Standaert
authors.forename: T
authors.initials: T
authors.lastname: Ishizuka
authors.forename: B
authors.initials: B
authors.lastname: Yu
authors.forename: H
authors.initials: H
authors.lastname: Hernandez
authors.forename: C
authors.initials: C
authors.lastname: Waldron
authors.forename: J
authors.initials: J
authors.lastname: Watson
authors.forename: J P
authors.initials: JP
authors.lastname: Farese
authors.forename: D R
authors.initials: DR
authors.lastname: Cooper
authors.forename: E
authors.initials: E
authors.lastname: Wickstrom
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D016373:DNA, Antisense / Q000235:genetics / Q000494:pharmacology*; D003847:Deoxyglucose / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D007329:Insulin Antagonists / Q000494:pharmacology; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D016373:DNA, Antisense; D007329:Insulin Antagonists; D007527:Isoenzymes; D003847:Deoxyglucose; D011493:Protein Kinase C; D005947:Glucose