Affinity chromatography of Escherichia coli (m5U54)-methyltransferase on tRNA-agarose.
tRNA-agarose was prepared by condensing periodate-oxidized tRNA to an agarose matrix containing hydrazide functional groups. The tRNA-agarose was used to take partially purified tRNA (m5U54)-methyltransferase to homogeneity. The method is simple and reproducible and gives high yields.
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Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
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D002482:Cellulose / Q000031:analogs & derivatives; D011232:Chemical Precipitation; D002846:Chromatography, Affinity / Q000379:methods*; D004926:Escherichia coli / Q000201:enzymology*; D011094:Polyethyleneimine; D012343:RNA, Transfer; D012685:Sepharose; D012359:tRNA Methyltransferases / Q000302:isolation & purification*
D011094:Polyethyleneimine; D002482:Cellulose; D012685:Sepharose; D012343:RNA, Transfer; C008881:phosphocellulose; D012359:tRNA Methyltransferases; C022769:tRNA(uracil-5)-methyltransferase