[Mutations in the 61st codon of the c-Ki-ras oncogene during transplacental lung tumor induction in mice and their difference in spontaneous and induced tumors].
DNA isolated from lung and liver tumor which were induced in CD-1 mice by transplacental treatment with 7,12-dimethylbenz(a)anthracene (DMBA) or developed spontaneously was analyzed for the presence of Ha- and Ki-ras oncogene codon 61 mutations. The A to T transversions at the second position of Ha-ras codon 61 were revealed only in liver tumors of DMBA-exposed animals, whereas only Ki-ras mutations occurred in both spontaneous and induced tumors of the lung. A to T mutations at the second position of Ki-ras codon 61 or non-identified yet mutations at the third position of the same codon were shown to be related to DMBA treatment. Thus both tissue and carcinogen specificity of ras oncogene activation was clearly demonstrated.
Loktionov
A S
AS
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000818:Animals; D001483:Base Sequence; D003062:Codon; D005260:Female; D011905:Genes, ras; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics / Q000473:pathology; D008175:Lung Neoplasms / Q000139:chemically induced* / Q000235:genetics / Q000473:pathology; D008431:Maternal-Fetal Exchange; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D011247:Pregnancy
D003062:Codon; D015127:9,10-Dimethyl-1,2-benzanthracene
Mutatsii v 61-m kodone onkogena c-Ki-ras pri transplatsentarnoĭ induktsii opukholeĭ legkikh u mysheĭ i ikh razlichiia v spontannykh i indutsirovannykh opukholiakh.