The palmitic acid binding properties of cytosolic proteins located in the villus and crypt zones of bovine intestinal mucosa.
The palmitic acid binding capacity of cytosolic proteins in three preparations obtained by differential scraping of bovine intestinal mucosa were compared. The data indicated that the palmitic acid binding activities depended on the position that the cells occupied along the crypt-villus axis, as shown from the level of alkaline phosphatase activity. Proteins with palmitate binding properties in the high- and low-molecular-weight cytosolic proteins in the villus zone bound 1.24 +/- 0.41 and 1.54 +/- 0.16 pmol palminate/micrograms protein respectively. The binding decreased to 0.50 +/- 0.25 and 1.10 +/- 0.23 pmol palmitate/micrograms for the proteins in the crypt zone. Ammonium sulphate fractionation and gel filtration chromatography indicated that the low-molecular-weight cytosolic proteins obtained from light mucosal scrapings contained the highest palmitate binding activity. These results suggest that the cytosolic proteins located in the villus zone may play a role in the absorption of fatty acids.
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Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Argentina.
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D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism*; D002417:Cattle / Q000378:metabolism*; D002850:Chromatography, Gel; D003600:Cytosol / Q000201:enzymology / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins; D005260:Female; D007413:Intestinal Mucosa / Q000201:enzymology / Q000378:metabolism*; D008871:Microvilli / Q000201:enzymology / Q000378:metabolism; D008970:Molecular Weight; D009363:Neoplasm Proteins; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*
D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D010169:Palmitic Acids; D019308:Palmitic Acid; D000469:Alkaline Phosphatase