title: Determination of the quaternary compound ciclotropium in human biological material after hydrolysis and derivatization with the fluorophor flunoxaprofen chloride.
abstract: The quantitative determination of the quaternary spasmolytic compound ciclotropium and its metabolite N-isopropyltropinium is described for human plasma and urine. The analytical procedure consists of ion-pair extraction from biological material, alkaline hydrolysis, subsequent derivatization with the fluorophor flunoxaprofen chloride and separation by high-performance liquid chromatography on a reversed-phase column with fluorimetric monitoring. The detection limits of 0.5 ng/ml in plasma and 10 ng/ml in urine at signal-to-noise ratios higher than 3 permit the determination of pharmacokinetic parameters after therapeutic doses.
authors.affiliation: Pharmakologisches Institut für Naturwissenschaftler, Johann Wolfgang Goethe-Universität, Frankfurt am Main, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Liebmann
authors.forename: D
authors.initials: D
authors.lastname: Henke
authors.forename: H
authors.initials: H
authors.lastname: Spahn-Langguth
authors.forename: E
authors.initials: E
authors.lastname: Mutschler
mesh_terms: D001583:Benzoxazoles / Q000737:chemistry*; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006868:Hydrolysis; D008297:Male; D010276:Parasympatholytics / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013696:Temperature; D014326:Tropanes / Q000652:urine
chemical_list: D001583:Benzoxazoles; D019086:Bridged Bicyclo Compounds, Heterocyclic; D010276:Parasympatholytics; D014326:Tropanes; C074867:N-isopropyltropinium; C040095:cyclotropium; C045356:flunoxaprofen