Influence of blood proteins in the in vitro adhesion of Staphylococcus epidermidis to teflon, polycarbonate, polyethylene and bovine pericardium.
The influence of human plasma proteins (fibrinogen, albumin and fibronectin) on the adherence of Staphylococcus epidermis to teflon, polyethylene, polycarbonate and bovine pericardium was studied in an in vitro quantitative assay by scintillation counting. Bacterial adhesion was generally reduced by the presence of protein during the adherence assay except in the case of bovine pericardium, in which adherence remained almost unaffected. The effect of these plasma proteins on bacterial surface properties resulted in strong increases of surface charge as measured by ion-exchange chromatography and with no effect on hydrophobicity, estimated as contact angles. Adherence was not found to be correlated with these two properties, suggesting that bacteria-surface interactions must not be simplified to the influence of interfacial forces.
Carballo
J
J
Departamento de Microbiología y Parasitología, Facultad de Farmacia, Santiago de Compostela, Spain.
Ferreirós
C M
CM
Criado
M T
MT
D000327:Adsorption; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D001672:Biocompatible Materials; D002417:Cattle; D005340:Fibrinogen / Q000494:pharmacology*; D005353:Fibronectins / Q000494:pharmacology*; D006801:Humans; D010496:Pericardium / Q000502:physiology; D011076:Polycarboxylate Cement; D011095:Polyethylenes; D011138:Polytetrafluoroethylene; D018805:Sepsis / Q000382:microbiology; D012709:Serum Albumin / Q000494:pharmacology*; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D013499:Surface Properties
D001672:Biocompatible Materials; D005353:Fibronectins; D011076:Polycarboxylate Cement; D011095:Polyethylenes; D012709:Serum Albumin; C005506:polycarbonate; D005340:Fibrinogen; D011138:Polytetrafluoroethylene