Pertussis toxin-insensitive regulation of phosphatidylinositol hydrolysis by vanadate in brain microvessels.
Vanadate, over a concentration range from 0.1 to 0.5 mM, stimulated the incorporation of (32P)-orthophosphate into PI and PA in brain microvessels. At concentrations higher than 0.5 mM, the stimulatory effect of vanadate decreased. Concommitantly, an enhanced DAG production was observed, indicating that vanadate stimulated PI turnover. All these effects were evident at all the times tested. Experiments performed in the presence of pertussis toxin (IAP) indicated that a IAP-sensitive G-protein does not mediate the vanadate stimulated PI effect in brain microvessels.
Catalán
R E
RE
Departamento de Biología Molecular, Centro de Biología Molecular (CSIC-UAM), Madrid, Spain.
Martínez
A M
AM
Aragonés
M D
MD
Miguel
B G
BG
Díaz
G
G
Hernández
F
F
D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects; D001921:Brain / Q000098:blood supply*; D004075:Diglycerides / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006868:Hydrolysis; D008297:Male; D008833:Microcirculation / Q000378:metabolism; D037342:Pertussis Toxin; D010710:Phosphates / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014638:Vanadates / Q000494:pharmacology*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D004075:Diglycerides; D010710:Phosphates; D010712:Phosphatidic Acids; D010716:Phosphatidylinositols; D010566:Virulence Factors, Bordetella; D014638:Vanadates; D037342:Pertussis Toxin