An improved fixation method for transmission electron microscopy for the histological study of the lens.
An improved fixation technique for transmission electron microscopic observation that enables good fixation of all areas of the rat lens was devised. Immersion fixation with 0.1 M phosphate-buffered 1.25% glutaraldehyde at 4 degrees C for 12 hours followed by postosmication produced good results for all areas of the lens--anterior, equatorial, and posterior zones. The technique was particularly suitable for maintenance of the shape of the lens since practically no irregularity, vacuolar degeneration, or expansion of the intercellular spaces was noted. This technique, which requires only a relatively short time, was also useful for the detection of early ultrastructural changes associated with cataract in spontaneously diabetic WBN/Kob rats. We anticipate that our procedure will be widely applied.
Sakai
H
H
Department of Ophthalmology, Kyorin University School of Medicine, Tokyo.
Takata
K
K
Fukuda
M
M
Fujiwara
T
T
Hirano
H
H
D000818:Animals; D002021:Buffers; D003080:Cold Temperature; D005976:Glutaral; D016591:Histocytological Preparation Techniques; D007908:Lens, Crystalline / Q000648:ultrastructure*; D008297:Male; D009993:Osmium Tetroxide; D010710:Phosphates; D051381:Rats; D011919:Rats, Inbred Strains; D016707:Tissue Fixation / Q000379:methods*
D002021:Buffers; D010710:Phosphates; D009993:Osmium Tetroxide; D005976:Glutaral