[The efficiency of the action of alpha-tocopherol and its homologs on luminol-dependent chemiluminescence induced by (Fe2+ + NADP.H) and (Fe2+ + ascorbate) systems in rat liver microsomes].
The effects of alpha-tocopherol (C16) and its homologues with different chain length (6-hydroxychromanes-C1, C6, C11) on lipid peroxidation induced luminol-dependent chemiluminescence in rat liver microsomal suspensions were studied. It was shown that C1, C6 and C11 inhibited the (Fe(2+) + ascorbate)-and (Fe(2+) + NADP.H)-induced chemiluminescence. The inhibitory effect was decreased in the order: C1 C6 C11, C16 was not influenced chemiluminescence. The possible reason underlying these differences was discussed: different efficiency of interaction of C16 and its homologues with hydroxyl and superoxide radicals, which initiate the luminol-dependent chemiluminescence. It was concluded that C16 (in concentration below 0.5 mM) was not interacted with hydroxyl and superoxide free radicals, generated in microsomal suspensions under (Fe(2+) + ascorbate)- and (Fe(2+) + NADP.H)-dependent lipid peroxidation.
Bakalova
R
R
Sokolova
Ts
Ts
Ribarov
S
S
Kagan
V
V
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002839:Chromans / Q000494:pharmacology*; D003864:Depression, Chemical; D005296:Ferrous Compounds / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism; D009249:NADP / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology*
D000975:Antioxidants; D002839:Chromans; D005296:Ferrous Compounds; D014810:Vitamin E; C020748:ferrous sulfate; D009249:NADP; D008165:Luminol; D001205:Ascorbic Acid
Effektivnost' deĭstviia al'fa-tokoferola i ego gomologov na liuminolzavisimuiu khemiliuminestsentsiiu, indutsiruemuiu sistemami (Fe+ + NADF.H) + (Fe2+ + askorbat) v mikrosomakh pecheni krys.