Characterization of immunoreactive pancreastatin in porcine tissues.
Pancreastatin is a 49 amino acid peptide with a C-terminal glycine amide originally isolated from porcine pancreas. There are strong indications that pancreastatin is derived from chromogranin A, since the amino acid sequence 240-288 in porcine chromogranin A contains pancreastatin flanked by typical signals for proteolytic processing. In the present study the distribution and molecular nature of immunoreactive pancreastatin were examined in selected porcine tissues. For this purpose a radioimmunoassay specific for the C-terminal sequence of porcine pancreastatin, that did not cross-react with porcine chromogranin A was used in combination with gel permeation chromatography and reverse-phase high-pressure liquid chromatography (HPLC). We demonstrated the presence of pancreastatin, a C-terminal pancreastatin fragment and N-terminally extended molecular forms in the examined tissues. Pancreastatin predominated in the pancreas and stomach antrum, while N-terminally extended molecular forms were mainly present in the stomach body, jejunum and adrenal gland. The specific distribution pattern of the molecular forms probably reflects a tissue-specific processing of chromogranin A.
Børglum Jensen
T D
TD
Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen, Denmark.
Holst
J J
JJ
Fahrenkrug
J
J
D000311:Adrenal Glands / Q000737:chemistry; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D053379:Chromogranin A; D002864:Chromogranins / Q000378:metabolism; D007583:Jejunum / Q000737:chemistry; D010179:Pancreas / Q000737:chemistry; D010187:Pancreatic Hormones / Q000032:analysis* / Q000737:chemistry; D010446:Peptide Fragments / Q000032:analysis; D011706:Pyloric Antrum / Q000737:chemistry; D011863:Radioimmunoassay / Q000592:standards / Q000706:statistics & numerical data; D013270:Stomach / Q000737:chemistry; D013552:Swine; D014018:Tissue Distribution
D053379:Chromogranin A; D002864:Chromogranins; D010187:Pancreatic Hormones; D010446:Peptide Fragments; C050836:pancreastatin