[Research on adducts of benzo(a)pyrene and DNA in different cells using synchronous fluorescence spectrophotometry].
The levels of benzo(a)pyrene diolepoxide(BPDE)-DNA adducts were measured in rat and human cells by synchronous fluorescence spectrophotometry. BPDE-DNA adducts detected in human pulmonary alveolar macrophages were related to current smoking habits, in contrast to the adducts found in peripheral blood leukocytes. BP administration to rats produced BPDE-DNA adducts in both liver and lungs. Although small yet repeatable signals were also detected in lung DNA from rats treated for 3 days with tobacco smoke. None of the samples obtained from untreated animals was positive. The detection of BPDE-DNA adducts may be used in biomonitoring and experimental studies for determining of exposure to BP even when applied as a constituent of complex mixtures.
Izzotti
A
A
Istituto di Igiene e Medicina Preventiva, Facoltà di Medicina e Chirurgia, Università di Genova.
Balansky
R
R
Scatolini
L
L
Coscia
N
N
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000032:analysis*; D000818:Animals; D004247:DNA / Q000032:analysis*; D018736:DNA Adducts; D004249:DNA Damage; D006801:Humans; D007962:Leukocytes / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D016676:Macrophages, Alveolar / Q000737:chemistry; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012907:Smoking; D013050:Spectrometry, Fluorescence
D018736:DNA Adducts; C043931:benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D004247:DNA
Ricerca di addotti fra benzo(a)pirene diolepossido e DNA in differenti cellule mediante spettrofotometria a fluorescenza sincrona.