title: Inter-laboratory ring trial of in vitro DNA repair tests using rat hepatocytes: further testing and conclusive remarks.
abstract: The results of the extension of a collaborative study for the detection of chemical-induced DNA damage in rat hepatocytes in vitro are presented in this report. Three coded compounds, i.e. 1,4-butanediol dimethanesulphonate, hydrazine sulphate and sodium dichromate, were tested for DNA repair synthesis by seven different laboratories, either using autoradiographic procedures or the liquid scintillation counting technique. Inter-laboratory standardization was intentionally not requested in order to investigate the validity of each study design under routine conditions. 1,4-Butanediol dimethanesulphonate was clearly positive in most laboratories; sodium dichromate was generally positive, while the results on hydrazine sulphate were contradictory.
authors.affiliation: Dr Karl Thomae GmbH, Ciba-Geigy Ltd, Basle, Switzerland.
authors.forename: E C
authors.initials: EC
authors.lastname: Puri
authors.forename: M
authors.initials: M
authors.lastname: Baumeister
authors.forename: R
authors.initials: R
authors.lastname: Fautz
authors.forename: C M
authors.initials: CM
authors.lastname: Hechenberger
authors.forename: T
authors.initials: T
authors.lastname: Hertner
authors.forename: C
authors.initials: C
authors.lastname: Meli
authors.forename: H G
authors.initials: HG
authors.lastname: Miltenburger
authors.forename: B
authors.initials: B
authors.lastname: Schmid
authors.forename: R
authors.initials: R
authors.lastname: Schwegler
authors.forename: A M
authors.initials: AM
authors.lastname: Seeberg
mesh_terms: D000818:Animals; D001345:Autoradiography; D002066:Busulfan / Q000633:toxicity; D002478:Cells, Cultured; D002840:Chromates / Q000633:toxicity; D004249:DNA Damage; D004260:DNA Repair; D006834:Hydrazines / Q000633:toxicity; D007753:Laboratories / Q000592:standards; D008099:Liver / Q000166:cytology; D009152:Mutagenicity Tests / Q000379:methods / Q000592:standards*; D051381:Rats; D012588:Scintillation Counting
chemical_list: D002840:Chromates; D006834:Hydrazines; C029424:hydrazine; C016104:sodium bichromate; D002066:Busulfan