[Preservation of hearts by perfusion under pressure].
OBJECTIVE
Development of method used to fix hearts in a reduced time, with preservation of their structures and characteristics.


METHODS
Fifty normal human hearts were fixed in a system with four reservoirs, a roller pump, PVC tubes and a plastic recipient. This system was used to maintain the continuous injection by pressure of formaldehyde solution, to the interior of the heart.


RESULTS
The fixation was satisfactory, pré-fixation time (13.93 +/- 5.13 min) and fixation time (60.53 +/- 12.50 min) were reduced related to previously utilized methods.


CONCLUSIONS
The method was good, with decrease of fixation time and obtention of fixed hearts, with preservation of their structures and characteristics.
Jatene
F B
FB
Instituto do Coração do Hospital das Clínicas-FMUSP, São Paulo.
Magalhães
M H
MH
De Oliveira
P R
PR
Jatene
A D
AD
D000447:Aldehydes; D015925:Cryopreservation / Q000379:methods*; D006321:Heart; D006801:Humans; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion / Q000379:methods*; D011312:Pressure; D013997:Time Factors
D000447:Aldehydes
Técnica de preservação de corações através de perfusão sob pressão.