A miniaturized fibrinolytic assay for plasminogen activators.
This report describes a micro-clot lysis assay (MCLA) for evaluating fibrinolytic activity of plasminogen activators (PA). Fibrin clots were formed in wells of microtiter plates. Lysis of the clots by PA, indicated by change in turbidity (optical density, OD), was monitored with a microplate reader at five minutes intervals. Log-log plots of PA dilution versus endpoint, the time at which the OD value was halfway between the maximum and minimum value for each well, were linear over a broad range of PA concentrations (2-200 International units/ml). The MCLA is a modification and miniaturization of well established fibrinolytic methods. The significant practical advantages of the MCLA are that it is a simple, relatively sensitive, non-radioactive, quantitative, kinetic, fibrinolytic micro-technique which can be automated.
Lewis
M L
ML
KRUG Life Sciences, Houston, Texas 77058.
Nachtwey
D S
DS
Damron
K L
KL
D002863:Chromogenic Compounds; D003470:Culture Media; D005340:Fibrinogen / Q000378:metabolism; D005342:Fibrinolysis / Q000502:physiology*; D006801:Humans; D008904:Miniaturization; D010960:Plasminogen Activators / Q000032:analysis*
D002863:Chromogenic Compounds; D003470:Culture Media; D005340:Fibrinogen; D010960:Plasminogen Activators