The effect of osmotics on plant cells: a study using 1H NMR relaxation and self-diffusion of water.
The osmotic changes in root cells of Zea mays L. under the effect of mannitol (concentration range 1-15%; range of osmotic pressures: -0.13 to -2.01 MPa) were studied by measuring time and concentration dependence of water self-diffusion constant (Deff) and proton spin-lattice relaxation time (T1) using proton NMR relaxation spectroscopy. In addition, in vivo uptake of Mn2+ by roots after their incubation in mannitol solutions were studied. At low (less than or equal to 5%) and medium (5-10%) concentrations of the osmoticum dehydration takes place proportional to the concentration, whereas membrane destruction occurs at higher concentrations (10-15%). It seems that there is a distribution of cells within root tissue regarding their sensitivity to osmotic stress.
Anisimov
A V
AV
Institute of Biology AN USSR, Kazan.
Evarestov
A S
AS
Dautova
N R
NR
Ratković
S
S
D004058:Diffusion; D009682:Magnetic Resonance Spectroscopy; D008345:Manganese / Q000378:metabolism; D008353:Mannitol / Q000494:pharmacology*; D009997:Osmotic Pressure; D014867:Water / Q000378:metabolism*; D003313:Zea mays / Q000187:drug effects / Q000378:metabolism*
D014867:Water; D008353:Mannitol; D008345:Manganese