title: The amino acid sequence of a Bowman-Birk type proteinase inhibitor from faba beans (Vicia faba L.).
abstract: The amino acid sequence of a Bowman-Birk type proteinase inhibitor (FBI) from seeds of faba bean (Vicia faba L.) was determined by analysis of peptide fragments generated by reduction and S-carboxymethylation of enzymatically modified inhibitors, which were obtained from native FBI by limited proteolysis with TPCK-trypsin or TLCK-chymotrypsin at pH 3.5. The established sequence showed that FBI is highly homologous with Vicia angustifolia inhibitor (VAI0 but lacks the portion corresponding to the C-terminal 9 amino acids of VAI. The trypsin reactive-site peptide bond in FBI was also indicated to be Lys(16)-Ser(17) and the chymotrypsin reactive-site peptide bond to be Tyr(42)-Ser(43) by limited proteolysis with TPCK-trypsin or TLCK-chymotrypsin and by sequence comparison with other Bowman-Birk type inhibitors.
authors.affiliation: Department of Food Sciences, Faculty of Home Economics, Mukogawa Women's University, Hyogo.
authors.forename: T
authors.initials: T
authors.lastname: Asao
authors.forename: F
authors.initials: F
authors.lastname: Imai
authors.forename: I
authors.initials: I
authors.lastname: Tsuji
authors.forename: M
authors.initials: M
authors.lastname: Tashiro
authors.forename: K
authors.initials: K
authors.lastname: Iwami
authors.forename: F
authors.initials: F
authors.lastname: Ibuki
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D007887:Fabaceae / Q000737:chemistry*; D008969:Molecular Sequence Data; D010946:Plants, Medicinal; D011480:Protease Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
chemical_list: D011480:Protease Inhibitors