Isolation, identification and synthesis of locustamyoinhibiting peptide (LOM-MIP), a novel biologically active neuropeptide from Locusta migratoria.
A novel peptide termed locustamyoinhibiting peptide (LOM-MIP) was isolated from brain-corpora cardiaca-corpora allata-suboesophageal ganglion extracts of the locust, Locusta migratoria. The primary structure of this nonapeptide has been determined Ala-Trp-Gln-Asp-Leu-Asn-Ala-Gly-Trp-NH2. LOM-MIP suppresses the spontaneous contractions of the hindgut and oviduct of Locusta migratoria and of the hindgut of Leucophaea maderae. This novel peptide is, however, structurally different from leucomyosuppressin, a hindgut suppressing peptide isolated from Leucophaea maderae heads. LOM-MIP has a Gly-TrpNH2 carboxy-terminal in common with APGWamide, a penis retractor muscle inhibiting peptide isolated from the snail, Lymnea stagnalis. In addition, it shows carboxy-terminal sequence similarities with locust AKH II which ends in AGWamide. No sequence similarities were found with other vertebrate or invertebrate peptides. Synthetic LOM-MIP showed biological as well as chemical characteristics indistinguishable from those of native LOM-MIP.
Schoofs
L
L
Zoological Institute, Katholieke Universiteit Leuven, Belgium.
Holman
G M
GM
Hayes
T K
TK
Nachman
R J
RJ
De Loof
A
A
D000595:Amino Acid Sequence; D000818:Animals; D003335:Corpora Allata / Q000737:chemistry; D004064:Digestive System / Q000187:drug effects; D004068:Digestive System Physiological Phenomena; D006110:Grasshoppers / Q000502:physiology*; D007301:Insect Hormones / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D019476:Insect Proteins; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009417:Nerve Tissue / Q000737:chemistry*; D009479:Neuropeptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D009490:Neurosecretory Systems / Q000737:chemistry; D010057:Oviducts / Q000187:drug effects / Q000502:physiology; D012689:Sequence Homology, Nucleic Acid
D007301:Insect Hormones; D019476:Insect Proteins; D009479:Neuropeptides; C073324:locustamyoinhibiting peptide, Locusta migratoria