[The effect of diamines on the process of lysosome fusion with phagosomes in mouse peritoneal macrophages].
Diamines (DA), characterized by a general formula H2N-(CH2) n-NH2 in which n varies from 2 to 10, inhibit the phagosome-lysosome fusion in murine peritoneal macrophages. The DA concentration was 0.2, 0.5 and 1.0 mM. The inhibitory effect increased with increasing the number of CH2-groups in the DA molecule. It was suggested that DA could influence the lysosomal membrane state. An additional proof of such changes was obtained with 1,6-diphenyl-1,3,5-hexatrien (DPH) as a fluorescent probe. Lysosomes isolated from murine peritoneal macrophages by differential centrifugation were used. It was found that DPH fluorescence intensity in lysosomal membrane increased under the influence of DA.
Mozhenok
T P
TP
Bulychev
A G
AG
Kuznetsova
I M
IM
Braun
A D
AD
D000818:Animals; D003864:Depression, Chemical; D003959:Diamines / Q000494:pharmacology*; D004161:Diphenylhexatriene; D004305:Dose-Response Relationship, Drug; D005453:Fluorescence; D008247:Lysosomes / Q000187:drug effects* / Q000648:ultrastructure; D008264:Macrophages / Q000187:drug effects* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D010529:Peritoneal Cavity / Q000166:cytology; D010588:Phagosomes / Q000187:drug effects* / Q000648:ultrastructure; D013329:Structure-Activity Relationship
D003959:Diamines; D004161:Diphenylhexatriene
Vliianie diaminov na protsess sliianiia lizosom s fagosomami v peritoneal'nykh makrofagakh mysheĭ.