Mitogenic activity of Tulipa gesneriana lectins on mouse and human lymphocytes.
Tulipa gesneriana lectin-erythrocyte (TGL-E) which agglutinates mouse erythrocytes showed a potent mitogenic activity on mouse spleen cells and human peripheral blood lymphocytes, however, TGL-E had only slight mitogenic activity on mouse thymus cells. Its subunit alpha with a molecular weight (MW) of about 26,000 showed a potent mitogenic activity as did that of native lectin, but subunit beta with a MW of about 14,000 showed no activity, indicating that the mitogenic activity of TGL-E originates from subunit alpha. TGL-E stimulated T cell enriched spleen cells which passed through a nylon column, but not spleen cells from a nude mouse or spleen cells treated with anti-Thy 1.2 antibody and complement. Thus, TGL-E stimulates only mouse T cells but not B cells. The other lectin in tulip bulbs, Tulipa gesneriana lectin-yeast showed no mitogenic activity on mouse spleen, thymus cells or human paripheral blood lymphocytes.
Oda
Y
Y
Faculty of Pharmaceutical Sciences, Kinki University, Higashi-Osaka, Japan.
Tatsumi
Y
Y
Aonuma
S
S
D000818:Animals; D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D037121:Plant Lectins; D010944:Plants / Q000737:chemistry*
D037102:Lectins; D037121:Plant Lectins