title: Induction of cytochrome P450 isozymes in human amnion FL cells and its application to the biological detection of mutagens.
abstract: Using AHH, EROD, ECOD and APND as marker enzymes and 3-MC, beta-NF, NE and PB as inducers, inducible cytP450 IA and IIB gene expression was demonstrated in the human amnion FL cell line; these cells possess a broad spectrum of drug-metabolizing enzymes. Maximum induction was observed following co-treatment with 3-MC and NE. Both constitutive and induced AHH were proved to have the characteristics of cytP448-dependent mixed-function oxygenases. Induced cytP450 isozyme activity remained at a high level for 24-36 h after removal of the inducer. The induced FL cells were demonstrated to activate common promutagens/procarcinogens in UDS and ADPRT-mediated decrease of NAD content assay systems. This new design can be used as a simplified mutagen screening system: no supplemental liver microsomal activation system is needed.
authors.affiliation: Zhejiang Branch, CAMS, Hangzhou.
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Sun
authors.forename: Y
authors.initials: Y
authors.lastname: Dai
authors.forename: M
authors.initials: M
authors.lastname: Fang
authors.forename: X
authors.initials: X
authors.lastname: Chen
mesh_terms: D000650:Amnion / Q000166:cytology / Q000201:enzymology*; D015197:Carcinogenicity Tests; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004790:Enzyme Induction; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis*; D009152:Mutagenicity Tests
chemical_list: D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System