L-alanine transport by systems A and ASC in plasma membrane vesicles isolated from Ehrlich cells.
Plasma membrane vesicles were prepared from Ehrlich cells using two-phase system compartmentation. These vesicles accumulated L-alanine mainly by means of Na(+)-dependent transport systems A and ASC. The kinetic of both transport systems could be elucidated by specific inhibition of system A with methyl-aminoisobutyric acid.
Luque
P
P
Laboratorio de Bioquímica, Facultad de Ciencias, Universidad de Málaga, Spain.
Márquez
J
J
Núñez de Castro
I
I
Medina
M A
MA
D000409:Alanine / Q000378:metabolism*; D026905:Amino Acid Transport Systems; D026921:Amino Acid Transport Systems, Neutral; D000621:Aminoisobutyric Acids / Q000494:pharmacology; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism; D027981:Symporters; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D026905:Amino Acid Transport Systems; D026921:Amino Acid Transport Systems, Neutral; D000621:Aminoisobutyric Acids; D002352:Carrier Proteins; D009363:Neoplasm Proteins; D027981:Symporters; C052168:sodium-alanine cotransporter; C017911:2-(methylamino)isobutyric acid; D000409:Alanine