The clastogenic effect of pyrimethamine (Daraprim) on human chromosomes in lymphocyte cultures.
In this study the clastogenic effect of pyrimethamine (Daraprim), a folic acid antagonist used for the treatment of toxoplasmosis and malaria on human chromosomes, was investigated. Pyrimethamine was added to in vitro lymphocyte cultures at six different concentrations: 0.05 (normal therapeutic dose), 0.1, 0.2, 0.4, 0.8, and 1.6 mg/ml. No proliferation was observed in any of the cultures containing 1.6 mg/ml pyrimethamine. The results of the cytogenetic evaluations show that the frequency of breaks and gaps increase significantly in dose-dependent manner. Thus, pyrimethamine has a clastogenic effect on human chromosomes.
Egeli
U
U
Istanbul University, Istanbul Faculty of Medicine, Medical Genetics Branch of Internal Medicine Department, Turkey.
Erdogan
G
G
D000293:Adolescent; D000328:Adult; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D009153:Mutagens / Q000633:toxicity*; D011739:Pyrimethamine / Q000633:toxicity*
D009153:Mutagens; D011739:Pyrimethamine