[Studies on water soluble polysaccharides isolated from Tribulus terrestris L--purification and preliminary structural determination of heteropolysaccharide H].
Crude polysaccharides extracted from the stem and leaf of Tribulus terristris L after the removal of crude saponins are a mixture of heteropolysaccharides composed of Ara, Rha, Xyl, GalA, Gal, Glc and Man in molar ratios of 6.0:2.1:1:3.6:3.4:7.7:2.9. A homogeneous polysaccharide H obtained by gradation and purification contains Ara, Rha, Xyl, GalA. Gal and Glc. in molar ratios of 1.6:2.4:0.1:3.5:1.3:1. Its molecular weight was found to be 1 x 10(5). By means of pectinase and beta-D-galactosidase enzymolysis, periodate oxidation, Smith degradation, partial hydrolysis with acid, methylation, GC and GC-MS, the H contains alpha-D-GalA (1-4) and L-Rha (1-2) probably linked alternately as main chain with some L-Rha (1-2) side chains.
Huang
X L
XL
Department of Biology, Northeast Normal University, Changchun.
Zhang
Y S
YS
Liang
Z Y
ZY
D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification
D004365:Drugs, Chinese Herbal; D011134:Polysaccharides