ELISA using monoclonal antibody to human serum arylesterase.
ELISA for determining arylesterase content in human serum has been developed by the one-step sandwich method using 2 monoclonal antibodies. While the content of arylesterase in healthy adults was 81 +/- 25 mg/l, a decrease was observed in patients with liver cirrhosis, where the mean +/- SD was 37 +/- 7 mg/l. ELISA of human serum arylesterase correlated with the activity determined by a specific substrate assay we devised recently.
Kawai
H
H
Pharmaceuticals Research Center, Kanebo, Ltd., Osaka, Japan.
Yomoda
S
S
Inoue
Y
Y
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D012016:Reference Values
D000911:Antibodies, Monoclonal; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase