Microimplants of estradiol in the sexually dimorphic area of the hypothalamus activate ultrasonic vocal behavior in male Mongolian gerbils.
The hormonal control of ultrasonic vocal behavior in the male Mongolian gerbil was examined by comparing the behavioral effects of androgen with those of estrogen administered to the preoptic-anterior hypothalamic area (POA-AH) in castrates. By measuring radioactivity released from solid "floating" POA-AH microimplants (mean diameter, 141 microns) of testosterone (3H-T, mean weight, 880 ng) in Experiment 1, we found that the steroid had a concentration gradient which fell rapidly from the edge of the microimplant, suggesting restricted diffusion. Using floating microimplants in Experiment 2, we studied the effects of testosterone propionate (TP, 650 ng), estradiol-17 beta benzoate (EB, 439 ng), or cholesterol (C, 478 ng) on rates of a frequency modulated ultrasonic vocalization emitted during sexual interactions. The effects on the upsweep call were compared with those on sexual mounting. The upsweep rate remained significantly below precastration levels in C implanted males. EB reinstated upsweep calling within 5 days, 3 days earlier than TP microimplants. Mounting in EB implanted males was maintained at precastration levels, whereas TP implantation restored mounting to precastration levels only after 5 days. EB was effective in inducing ultrasonic vocalizations when placed in, or near, the sexually dimorphic area (SDA) in the medial preoptic area (POM). Our results indicate that brain mechanisms underlying both ultrasonic vocalizations and mounting are directly sensitive to estradiol (E2) in the male gerbil. We conclude that E2 affects mechanisms in the SDA associated with ultrasonic calling and suggest that T is likely to act via aromatization products in the brain.
Holman
S D
SD
MRC Neuroendocrine Development and Behaviour Group, Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, England.
Hutchison
R E
RE
Hutchison
J B
JB
D000818:Animals; D001931:Brain Mapping; D004958:Estradiol / Q000502:physiology*; D005849:Gerbillinae / Q000502:physiology*; D007031:Hypothalamus / Q000502:physiology*; D007032:Hypothalamus, Anterior / Q000502:physiology; D008297:Male; D011301:Preoptic Area / Q000502:physiology; D011944:Receptors, Androgen / Q000502:physiology; D011959:Receptors, Estradiol / Q000502:physiology; D012726:Sexual Behavior, Animal / Q000502:physiology*; D012741:Sexual Maturation; D013739:Testosterone / Q000502:physiology; D014465:Ultrasonics; D014828:Vocalization, Animal / Q000502:physiology*
D011944:Receptors, Androgen; D011959:Receptors, Estradiol; D013739:Testosterone; D004958:Estradiol