Direct up-regulation of estrogen receptor by triiodothyronine in rat pituitary tumor MtT/F84.
To investigate a possible effect of triiodothyronine (T3) on the regulation of estrogen receptor, estrogen dependent rat pituitary tumor, MtT/F84, was studied in rats which received surgical thyroidectomy (Tx) or were given propylthiouracil (PTU) and were supplemented with T3. In T3 loaded rats, the grafted tumor showed high estrogen receptor levels (160-200% of control), whereas low estrogen receptor levels (20-35% of control) were observed in the tumors grown in Tx and PTU treated rats. A single injection of T3 to Tx rats with MtT/F84 increased the estrogen receptor level in a time dependent manner and reached the maximal level at 6 h. In primary culture of MtT/F84 cells, T3 increased the specific 3H-estrogen binding to tumor cells in a dose dependent manner (165% of control by 10(-7)M T3) and also in a time dependent (maximum at 12 h) manner. These data suggest that T3 directly up-regulates the estrogen receptor level in MtT/F84 cells.
Fujimoto
N
N
Department of Cancer Research, Hiroshima University, Japan.
Watanabe
H
H
Ito
A
A
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000378:metabolism; D004967:Estrogens; D013006:Growth Hormone / Q000097:blood; D066298:In Vitro Techniques; D009376:Neoplasms, Hormone-Dependent / Q000188:drug therapy / Q000378:metabolism* / Q000601:surgery; D010911:Pituitary Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000601:surgery; D011388:Prolactin / Q000097:blood; D011441:Propylthiouracil / Q000627:therapeutic use; D051381:Rats; D011916:Rats, Inbred F344; D011960:Receptors, Estrogen / Q000187:drug effects* / Q000378:metabolism; D013965:Thyroidectomy; D013997:Time Factors; D014284:Triiodothyronine / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000187:drug effects*
D004967:Estrogens; D011960:Receptors, Estrogen; D014284:Triiodothyronine; D004958:Estradiol; D011441:Propylthiouracil; D011388:Prolactin; D013006:Growth Hormone