Analyses of rabbit platelet proteo-chondroitin sulfates localized on the surface and in the granules.
Platelet proteo-chondroitin sulfates localize on the surface and in the granules. Western blotting method is able to selectively detect core-proteins prepared from proteo-chondroitin sulfates by chondroitinase ABC digestion. A very highly sensitive high-performance liquid chromatographic method was established for the determination of chondroitin sulfates as their unsaturated disaccharides. These methods were applied to rabbit platelet proteo-chondroitin sulfates. It was found that both rabbit platelet surface proteo-chondroitin sulfate and granule proteo-chondroitin sulfate consist of a predominantly homogeneous chondroitin 4-sulfate and a single core-protein having a molecular weight of approximately 26 kDa.
Imanari
T
T
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Oguma
T
T
Akiyama
H
H
Toyoda
H
H
Koshiishi
I
I
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003594:Cytoplasmic Granules / Q000378:metabolism*; D004588:Electrophoresis, Cellulose Acetate; D011817:Rabbits
D011508:Chondroitin Sulfate Proteoglycans