A comprehensive two-dimensional gel protein database of noncultured unfractionated normal human epidermal keratinocytes: towards an integrated approach to the study of cell proliferation, differentiation and skin diseases.
A two-dimensional (2-D) gel database of cellular proteins from noncultured, unfractionated normal human epidermal keratinocytes has been established. A total of 2651 [35S]methionine-labeled cellular proteins (1868 isoelectric focusing, 783 nonequilibrium pH gradient electrophoresis) were resolved and recorded using computer-aided 2-D gel electrophoresis. The protein numbers in this database differ from those reported in an earlier version due to changes in the scanning hardware (Celis et al., Electrophoresis 1990, 11, 242-254). Annotation categories reported include: "protein name" (listing 207 known proteins in alphabetical order), "basal cell markers", "differentiation markers", "proteins highly up-regulated in psoriatic skin", "microsequenced proteins" and "human autoantigens". For reference, we have also included 2-D gel (isoelectric focusing) patterns of cultured normal and psoriatic keratinocytes, melanocytes, fibroblasts, dermal microvascular endothelial cells, peripheral blood mononuclear cells and sweat duct cells. The keratinocyte 2-D gel protein database will be updated yearly in the November issue of Electrophoresis.
Celis
J E
JE
Institute of Medical Biochemistry, Aarhus University, Denmark.
Madsen
P
P
Rasmussen
H H
HH
Leffers
H
H
Honoré
B
B
Gesser
B
B
Dejgaard
K
K
Olsen
E
E
Magnusson
N
N
Kiil
J
J
D001706:Biopsy; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional; D004730:Endothelium, Vascular / Q000473:pathology; D006801:Humans; D015151:Immunoblotting; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D011565:Psoriasis / Q000473:pathology*; D012016:Reference Values; D013545:Sweat Glands / Q000473:pathology; D015854:Up-Regulation / Q000502:physiology
D011506:Proteins