Endothelin-1 secretion by human fibroblasts in culture: effects of cell density and IFN-beta.
Evidence is presented of ET-1 release by cultured human fibroblasts. Such a conclusion is supported by the parallelism in displacement curves obtained using dilutions of extracts prepared from fibroblast conditioned media and the synthetic ET-1 standards, and the significant time dependent increase in ET-1 content in media from IFN-beta treated human fibroblasts. An increase in cell density significantly elevated the total amount of ET-1 in the conditioned media, although a linear relationship between these two variables was not observed.
Zeballos
G A
GA
Department of Physiology, New York Medical College, Valhalla 10595.
An
S J
SJ
Wu
J M
JM
D002452:Cell Count; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D016232:Endothelins / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D016899:Interferon-beta / Q000494:pharmacology; D013268:Stimulation, Chemical
D003470:Culture Media; D016232:Endothelins; D016899:Interferon-beta