The effects of coexisting lipids on the action of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C toward liposomal substrate.
The hydrolytic activity of phosphatidylinositol (PI)-specific phospholipase C (PI-PLC) from Bacillus thuringiensis was studied in detail toward mixed liposomes consisting of PI and one of other phospholipids and cholesterol. Among PI-liposomes, small unilamellar vesicles (SUV) were the most sensitive to PI-PLC; the enzymatic hydrolysis of PI in SUV was not less than 10-fold that in large unilamellar vesicles (LUV) or in multilamellar vesicles (MLV). Thus, in a survey of the effects of coexisting lipids on PI-PLC activity, PI-SUV was used. Phosphatidylcholine (PC) was stimulative for the enzyme activity toward PI-SUV at any molar ratio of PC to PI. Also, the effects of the addition of sphingomyelin (SM), phosphatidylethanolamine (PE) and cholesterol on the enzymatic hydrolysis of PI were studied in detail on the basis of concentration of total lipids or PI.
Kume
T
T
Faculty of Pharmaceutical Sciences, Nagaya City University, Aichi, Japan.
Taguchi
R
R
Ikezawa
H
H
D001413:Bacillus thuringiensis / Q000201:enzymology*; D002784:Cholesterol / Q000737:chemistry; D008055:Lipids / Q000494:pharmacology*; D008081:Liposomes; D010716:Phosphatidylinositols / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism*
D008055:Lipids; D008081:Liposomes; D010716:Phosphatidylinositols; D002784:Cholesterol; D010738:Type C Phospholipases