Activation of hyaluronidase by 8-methoxypsoralen-polyamine photoproducts.
Ultraviolet radiation of 8-methoxypsoralen (8-MOP) in the presence of various polyamines resulted in stable photoproducts that were very soluble in water and showed hyaluronidase-activating properties. Among them, the photoproducts obtained from the reaction systems of 8-MOP-spermine and 8-MOP-spermidine markedly activated hyaluronidase. The enzyme activity was not affected by 8-MOP alone and the photoproduct of 8-MOP (8-MOP-P). From these facts, it was suggested that the photoproducts with hyaluronidase-activating properties might play an important role in the onset of 8-MOP-induced photosensitivity.
Shigehara
N
N
Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan.
Kakegawa
H
H
Miyataka
H
H
Matsumoto
H
H
Isobe
A
A
Satoh
T
T
D000818:Animals; D002417:Cattle; D002855:Chromatography, Thin Layer; D004789:Enzyme Activation / Q000187:drug effects; D006821:Hyaluronoglucosaminidase / Q000737:chemistry*; D008730:Methoxsalen / Q000494:pharmacology / Q000528:radiation effects*; D011073:Polyamines / Q000494:pharmacology / Q000528:radiation effects*; D014466:Ultraviolet Rays
D011073:Polyamines; D006821:Hyaluronoglucosaminidase; D008730:Methoxsalen