title: Molecular heterogeneity of platelet-activating factor (PAF) in rat glandular stomach determined by gas chromatography/mass spectrometry. PAF molecular species changes upon water-immersion stress.
abstract: The molecular heterogeneity of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine (alkylacetyl-GPC) and 1-acyl-2-acetyl-sn-glycero-3-phosphocholine (acylacetyl-GPC) in normal rat glandular stomach was studied by gas chromatography/mass spectrometry (GC/MS) and tandem mass spectrometry. The percentage compositions of the molecular species of 1-alkyl-2-acetyl-GPC and 1-acyl-2-acetyl-GPC in the antrum were, respectively, 1-alkyl [16:0 (34%) and 18:0 (66%)]-2-acetyl-GPC and 1-acyl [16:0 (60%), 18:0 (14%) and 18:1 (26%)]-2-acetyl-GPC. The alkyl chain composition of 1-alkyl-2-acetyl-GPC was quite different from that of 1-alkyl-2-acyl-GPC in both the antrum and corpus, demonstrating a high degree of selectivity of alkyl chain utilization in PAF biosynthesis. The amount of 1-acyl-2-acetyl-GPC was much greater than that of 1-alkyl-2-acetyl-GPC. The molecular heterogeneity of 1-alkyl-2-acetyl-GPC and 1-acyl-2-acetyl-GPC in the corpus was similar to that in the antrum. Water-immersion stress affected not only the amount of 1-alkyl-2-acetyl-GPC and 1-acyl-2-acetyl-GPC, but also their molecular heterogeneity in the antrum and corpus. Whereas the amounts of 1-hexadecyl-2-acetyl-GPC and 1-acyl [16:0, 18:0 and 18:1]-2-acetyl-GPC decreased markedly (to less than one-fifth) in the antrum after such stress for 1 hr, the amount of 1-octadecyl-2-acetyl-GPC increased markedly (up to 4-fold) in the corpus and severe lesions were observed after stress for 7 hr.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Medical Chemistry, Kansai Medical School, Osaka, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Sugatani
authors.forename: K
authors.initials: K
authors.lastname: Fujimura
authors.forename: M
authors.initials: M
authors.lastname: Miwa
authors.forename: K
authors.initials: K
authors.lastname: Satouchi
authors.forename: K
authors.initials: K
authors.lastname: Saito
mesh_terms: D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007101:Immersion; D008297:Male; D010713:Phosphatidylcholines / Q000302:isolation & purification / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000302:isolation & purification / Q000378:metabolism; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013270:Stomach / Q000503:physiopathology*; D013315:Stress, Psychological / Q000503:physiopathology*
chemical_list: D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010972:Platelet Activating Factor