title: A scaffold-associated DNA region is located downstream of the pea plastocyanin gene.
abstract: Chromosomal scaffold-associated DNA has been isolated from pea leaf nuclei treated with lithium diiodosalicylate to remove histones and then digested with restriction enzymes to remove the DNA in chromosomal loops. A scaffold-associated region (SAR) of DNA has been identified 8 to 9 kb downstream of the single-copy pea plastocyanin gene in proximity to a repetitive sequence present in 300 copies in the pea haploid genome. Isolated restriction fragments from within the SAR can bind to scaffold preparations in a binding assay in vitro. The nucleotide sequence of the SAR indicates a 540-bp 77% A+T-rich region containing many sequence elements in common with SARs from other organisms. Sequences with homology to topoisomerase II binding sites, A-box and T-box sequences, and replication origins are present within this AT-rich region.
authors.affiliation: Botany School, University of Cambridge, United Kingdom.
authors.forename: R E
authors.initials: RE
authors.lastname: Slatter
authors.forename: P
authors.initials: P
authors.lastname: Dupree
authors.forename: J C
authors.initials: JC
authors.lastname: Gray
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D007887:Fabaceae / Q000235:genetics; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics*; D010946:Plants, Medicinal; D010970:Plastocyanin / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
chemical_list: D004247:DNA; D010970:Plastocyanin