[DNA replication in mammalian cells after the action of physical, chemical or biological factors. VI. The recovery of DNA synthesis in a culture of irradiated cells].
The kinetics of DNA synthesis restoration in cultured HeLa cells and in L-929 mouse fibroblasts irradiated by gamma-rays of 60Co with a dose of 10 Gy was studied. Early after irradiation the rate of DNA synthesis in HeLa cells measured with 3H-thymidine incorporation was seen to decrease. Two hours later the incorporation starts to increase to reach the control level 4 hours after irradiation and then becomes even higher than this level. The distribution of cells among phases of the cell cycle measured with flow cytometry undergoes changes. 4-6 hours after irradiation part of S-phase cells increased contributing presumably to the elevating of 3H-thymidine incorporation observed at this time. The restoration of the incorporation was suppressed by inhibitors of protein and RNA synthesis--cycloheximide and actinomycin D. It is suggested that the processes of restoration of DNA synthesis in irradiated cells can be of inducible nature. In irradiated HeLa and L-929 cells the restoration of DNA synthesis is resistant to novobiocin, an inhibitor of DNA replication.
Smirnov
S G
SG
Synzynys
B I
BI
Zamulaeva
I A
IA
Matylevich
N P
NP
Afanas'ev
V N
VN
Pechatnikhov
V A
VA
Saenko
A S
AS
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects / Q000528:radiation effects*; D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects*; D003609:Dactinomycin / Q000494:pharmacology; D005434:Flow Cytometry; D005720:Gamma Rays; D006367:HeLa Cells; D006801:Humans; D007739:L Cells; D051379:Mice; D009675:Novobiocin / Q000494:pharmacology; D013997:Time Factors
D009675:Novobiocin; D003609:Dactinomycin; D004247:DNA; D003513:Cycloheximide
Replikatsiia DNK v kletkakh mlekopitaiushchikh posle deĭstviia faktorov fizicheskoĭ, khimicheskoĭ ili biologicheskoĭ prirody. VI. Vosstanovlenie sinteza DNK v kul'ture obluchennykh kletok.