title: Purification, characterization and comparison with mammalian SCP2 of a chicken SCP2-like protein.
abstract: 1. Three proteins have been isolated from chicken (Gallus domesticus) liver that bind antibodies directed against authentic rat sterol carrier protein2 (SCP2) and have similar molecular mass to the three major immunoreactive rat liver proteins (12 kDa, 30-36 kDa, 55-60 kDa). 2. Bile from both chicken and rat contains the high molecular mass immunoreactive species. 3. The chicken 12 kDa SCP2-like protein purifies similarly to rat SCP2 but the homogeneous chicken SCP2-like protein is dissimilar in amino acid composition and N-terminal amino acid sequence. 4. The activity of chicken SCP2-like protein differs from rat SCP2 in that it was consistent with fusion (transfer of both polar surface and non-polar core lipids) rather than transfer of polar lipids only.
authors.affiliation: US Department of Agriculture, Eastern Regional Research Center, Philadelphia, PA 19118.
authors.forename: M P
authors.initials: MP
authors.lastname: Reinhart
authors.forename: S J
authors.initials: SJ
authors.lastname: Avart
authors.forename: T
authors.initials: T
authors.lastname: Foglia
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001646:Bile / Q000737:chemistry; D015153:Blotting, Western; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002417:Cattle; D002645:Chickens; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000737:chemistry; D008969:Molecular Sequence Data; D010940:Plant Proteins; D051381:Rats; D013261:Sterols / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D010940:Plant Proteins; D013261:Sterols; C072087:sterol carrier proteins