Regulation of neuronal differentiation in neuron-enriched primary cultures from embryonic rat cerebra by platelet activating factor and the structurally related glycerol ether lipid, dodecylglycerol.
Primary cultures enriched in neurons dissociated from embryonic rat cerebra were used to demonstrate that platelet activating factor and the structurally related ether glycerolipid, dodecylglycerol, are readily taken up in small amounts by neurons and that they stimulate the differentiation of neurons. The stimulation of neuronal differentiation was observed as a precocious development of axon-like extensions which correlated with a concentration-dependent increase in neuronal-specific enzyme activities. This stimulation of morphological and neurochemical factors by either platelet activating factor or dodecylglycerol was almost completely abolished by triazolam, a known inhibitor of platelet activating factor function. Neither platelet activating factor nor dodecylglycerol at the concentrations used to achieve stimulation of neuronal differentiation compromised the plasma membrane, as indicated by the lack of leakage of cytoplasmic lactic acid dehydrogenase.
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Department of Biochemistry, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Gustow
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Pieringer
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RA
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000166:cytology* / Q000196:embryology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003593:Cytoplasm / Q000201:enzymology; D005260:Female; D005989:Glycerides / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007848:Laurates / Q000494:pharmacology*; D050178:Monoglycerides; D009474:Neurons / Q000166:cytology*; D010972:Platelet Activating Factor / Q000494:pharmacology*; D011247:Pregnancy; D051381:Rats; D014229:Triazolam / Q000494:pharmacology
D005989:Glycerides; D007848:Laurates; D050178:Monoglycerides; D010972:Platelet Activating Factor; D014229:Triazolam; C020777:monolaurin; D007770:L-Lactate Dehydrogenase; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase