Thiol reduction-mediated 99mTc-IgG for scintigraphy of inflammatory lesions in the baboon model.
Radiolabeling procedures may modify the structure of the Fc portion of the immunoglobulin molecule in such a way that its in vivo immunological behavior may be altered and its efficacy as radiopharmaceutical for inflammatory lesions impaired. This study tested the efficacy of thiol reduction-mediated 99mTc human IgG for scintigraphy of focal inflammatory lesions, either bacterially or chemically induced and located either in the abdominal/thoracical region or in the thigh of baboons. Positive images were obtained in the thigh lesions between 4 and 7 hr after i.v. administration of the labeled IgG. The abdominal/thoracic lesions were never very clear, mostly because of very hot kidneys. Late visualization (20 hr) of all lesions was poor and a high background was present. Bacterially induced lesions were better visible, although no neutrophil nor monocyte activity could be established in the mechanism of the IgG localization.
Dormehl
I C
IC
AEC Institute for Life Sciences, University of Pretoria, South Africa.
Louw
W K
WK
Hugo
N
N
Redelinghuys
I F
IF
Fourie
P J
PJ
D000818:Animals; D005557:Formaldehyde; D007074:Immunoglobulin G; D007249:Inflammation / Q000139:chemically induced / Q000000981:diagnostic imaging* / Q000382:microbiology; D007553:Isotope Labeling; D008297:Male; D015609:Organotechnetium Compounds; D010215:Papio; D011877:Radionuclide Imaging; D013203:Staphylococcal Infections / Q000150:complications
D007074:Immunoglobulin G; D015609:Organotechnetium Compounds; C075091:technetium Tc 99m thiol reduction-mediated IgG; D005557:Formaldehyde