Effect of tumour-promoting phorbol ester on calcium homeostasis in human platelets.
The more interesting features of the effects or PMA on [Ca2+]i and ATP release were the following: 1. preincubation with PMA inhibited thrombin-evoked calcium transients; 2. PMA stimulated slightly the release of calcium and ATP whereas inhibited calcium and ATP pools sensitive to thrombin; 3. A23187 reversed the inhibitory effect of PMA; 4. subsaturating thrombin concentrations gave results similar to PMA on thrombin-induced calcium and ATP release but not on [Ca2+]i.
Lucas
M
M
Departmento de Bioquímica, Hospital Universitario Virgen Macarena, Facultad de Medicina, Sevilla, Spain.
Solano
F
F
D000255:Adenosine Triphosphate / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology; D006801:Humans; D015539:Platelet Activation / Q000187:drug effects / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013917:Thrombin / Q000378:metabolism
D000001:Calcimycin; D000255:Adenosine Triphosphate; D013917:Thrombin; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium