[Mapping the genes of the vaccinia virus, coding membrane proteins p34 and p40, by mRNA hybridization selection].
The HindIII--J HindIII-F fragments of the vaccinia virus DNA strain Lister have been analysed by the technique of mRNA hybridization selection with the subsequent translation in cell-free protein synthesizing system from the rabbit reticulocytes. The viral mRNA hybridizable with the HindIII--J fragment was shown to direct the synthesis of 30 kDa polypeptide in the cell-free system. This polypeptide was demonstrated to react specifically with antiserum to plasma membrane protein p34. The viral mRNA hybridizable with the HindIII-F fragment was shown to direct the synthesis of 37 kDa polypeptide in the cell-free system. This polypeptide reacts specifically with antiserum to major membrane protein p40.
Cheshenko
N V
NV
Netesova
N A
NA
Muravlev
A I
AI
Malygin
E G
EG
D002474:Cell-Free System; D003001:Cloning, Molecular; D005814:Genes, Viral; D008565:Membrane Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D015183:Restriction Mapping; D014616:Vaccinia virus / Q000235:genetics*
D008565:Membrane Proteins; D012333:RNA, Messenger; D012367:RNA, Viral
Kartirovanie genov virusa ospovaktsiny, kodiruiushchikh membrannye belki p34 i p40, metodom gibridizatsionnoĭ selektsii mRNK.