title: Increased insulin responsiveness in isolated rat hepatocytes incubated with free fatty acid-poor albumin.
abstract: Freshly isolated rat hepatocytes incubated in a medium containing bovine serum albumin partially purified by charcoal treatment at pH 3 are three times more responsive to insulin with regard to alpha-aminoisobutyric acid uptake than those incubated in medium containing regular bovine serum albumin. This finding should facilitate future work in isolated hepatocytes, a cell system that for the most part has been considered relatively unresponsive to insulin.
authors.affiliation: Department of Medicine, East Carolina University School of Medicine, Greenville, North Carolina.
authors.forename: J F
authors.initials: JF
authors.lastname: Caro
authors.forename: J
authors.initials: J
authors.lastname: Hodges
authors.forename: M K
authors.initials: MK
authors.lastname: Sinha
mesh_terms: D000621:Aminoisobutyric Acids / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D011972:Receptor, Insulin / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000494:pharmacology*
chemical_list: D000621:Aminoisobutyric Acids; D016895:Culture Media, Serum-Free; D005230:Fatty Acids, Nonesterified; D007328:Insulin; C100049:2-aminoisobutyric acid; D012710:Serum Albumin, Bovine; D011972:Receptor, Insulin