Studies on breeding of antibiotic-producing strain 5102 by protoplast fusion. IV: Verification of fusant FR-008, and isolation and characterization of the new antimicrobial substance.
On the basis of the high performance liquid chromatography (HPLC) of Antibiotic 5102-1, the principal components of this antibiotic were isolated from the cultures of starting strain 10-22 and the fusant FR-008. The result further verified that FR-008 was a recombinant of intraspecific fusion of Streptomyces hygroscopicus subsp. yingchengensis. A new antimicrobial substance was isolated and purified from the fermented product of fusant FR-008, which could not be produced by the starting strain. The color reactions of the new substance with concentrated sulphuric acid or concentrated hydrochloric acid and the UV-VIS absorptions indicated that it was a haptene macrolide antibiotic. The liquid chromatography for aromatic moiety showed that this antibiotic contained p-amino-acetophenone moiety. The amino acid analysis of the antibiotic revealed that it contained an aminosugar moiety, which was different from that in any other haptene macrolide antibiotic. The detailed chemical structure of the aminosugar moiety has yet to be identified.
Yuan
D J
DJ
Agricultural Antibiotic Laboratory, Huazhong Agricultural University, Wuhan.
Zhou
Q
Q
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D018942:Macrolides; D011523:Protoplasts / Q000378:metabolism*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013302:Streptomyces / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D018942:Macrolides