Acetylated low-density lipoproteins promote the release and metabolism of arachidonic acid by murine macrophages.
We studied the effect of acetyl-LDL on the synthesis of eicosanoids by primary cultures of mouse-resident peritoneal macrophages. A dosis of 150 micrograms of acetyl-LDL proteins promotes a maximal release of arachidonic acid metabolites into extracellular medium. This process is very rapid and reaches the maximal value about ten minutes after stimulation. The pattern of arachidonic acid metabolites released is different from that obtained when the cells are stimulated with a phagocytosable particle like zymosan or calcium ionophore A23187. The data show that the metabolism of arachidonic acid by cycloxigenase is diminished. In an unusual way the amounts of prostaglandin PGE2, 6-keto-prostaglandin PGF1 alpha and thromboxane TXB2 synthesized are similar.
Diez Monedero
E
E
Unidad de Lípidos y Arterosclerosis, Hopital Clínico de S. Carlos Universidad Complutense, Madrid, Spain.
Fernández
B
B
Martin
C
C
Zamorano
P
P
Gutierrez Fuentes
J A
JA
Serrano Ríos
M
M
Municio
A M
AM
Schüller
A
A
D000107:Acetylation; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice
D008077:Lipoproteins, LDL; D016718:Arachidonic Acid