[Action of estradiol-17 beta on the expression of c-Fos gene in endometrial cells in cultured].
The c-fos expression was investigated in primary culture of guinea-pig endometrial cells. Cells were made quiescent by serum depletion. Stimulation of these cells by estradiol (E2, 10(-8)M) alone or in combination with epidermal growth factor (EGF, 100 ng/ml) or insulin (10 micrograms/ml) failed to induce c-fos gene. The c-fos expression was early and transiently increased by fetal calf serum (15%) or estradiol plus EGF plus insulin. Protein synthesis inhibitors (cycloheximide or anisomycin) in association with E2 induced a superinduction of c-fos gene. In the same conditions puromycin had no effect. It appears that E2 acts in a multiple step process including an initial c-fos gene derepression by either EGF plus insulin or some protein synthesis inhibitors.
Adessi
G
G
INSERM U 198, Besançon.
Jouvenot
M
M
Vuillermoz
C
C
Pellerin
I
I
Royez
M
M
Maréchal
G
G
Breton
C
C
D000818:Animals; D002478:Cells, Cultured; D004338:Drug Combinations; D004717:Endometrium / Q000166:cytology*; D004815:Epidermal Growth Factor / Q000008:administration & dosage / Q000494:pharmacology; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D016762:Genes, fos / Q000235:genetics*; D006168:Guinea Pigs; D007328:Insulin / Q000008:administration & dosage / Q000494:pharmacology
D004338:Drug Combinations; D007328:Insulin; D004958:Estradiol; D004815:Epidermal Growth Factor
Action de l'estradiol-17 beta sur l'expression de c-fos dans les cellules d'endomètre en culture.