[A stereoscopic analysis of the centrosome structure in the cells of continuous and primary cell cultures].
A 3D reconstruction of the centrosome region was made based on series of semithick sections in tissue culture cells. It was shown that: 1) the total number of microtubules attached to the centrosome is about 30-50 of which only 20% or less run farther than 2 microns away from the centrosome; 2) a certain number of short microtubules (less than 1 micron length) is present in the vicinity of the centrosome, the majority of them are attached to the centrosome; 3) many microtubules around the centrosome have no direct contact with either centrioles, or other microtubule-convergent structures; 4) the majority of free microtubules are comparatively long (more than 1 micron length); 5) almost all the microtubules running closer than 2 microns to the centrosome are oriented towards it with their proximal ends. The radial distribution of free microtubules around the centrosome support the supposition that they may appear as a result of their detachment from the microtubule-nucleating centres.
Alieva
I B
IB
Vorob'eva
I A
IA
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000648:ultrastructure; D003584:Cytological Techniques; D003593:Cytoplasm / Q000648:ultrastructure*; D005347:Fibroblasts / Q000648:ultrastructure; D007668:Kidney / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D008870:Microtubules / Q000648:ultrastructure; D013552:Swine
Stereoskopicheskiĭ analiz stroeniia tsentrosomy v kletkakh perevivnoĭ i pervichnoĭ kletochnykh kul'tur.