title: Application of scale-down experiments in the study of citric acid biosynthesis.
abstract: Problems associated with the development of submersed citric acid biosynthesis on sugar beet molasses for process improvement were solved by scale-down experiments: the determination of optimal temperature profile, optimal pH value, influence of OTR and inoculum quality. The optimal pH value at the start of the process was 6.4 with 3% of inoculum volume and temperature profile between 28-30 degrees C. In the case of OTR which is scale-dependent, we prefer to use DOC pilot plant data for the transfer of technology.
authors.affiliation: Department of Biotechnology, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
authors.forename: P
authors.initials: P
authors.lastname: Ettler
authors.forename: L
authors.initials: L
authors.lastname: Martínková
authors.forename: E
authors.initials: E
authors.lastname: Ujcová
authors.forename: L
authors.initials: L
authors.lastname: Seichert
mesh_terms: D001234:Aspergillus niger / Q000378:metabolism; D001709:Biotechnology; D002951:Citrates / Q000096:biosynthesis*; D019343:Citric Acid; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010100:Oxygen; D013696:Temperature
chemical_list: D002951:Citrates; D019343:Citric Acid; D010100:Oxygen