Novel cell surface antigens expressed on mouse alveolar macrophages.
Two new cell surface antigens expressed on mouse alveolar macrophages were defined by rat monoclonal antibodies. One marker, AVM-1, was detected on mouse alveolar macrophages, but it was undetectable on resident peritoneal cells, thioglycollate medium-induced peritoneal cells, and splenic macrophages. Splenic lymphocytes, thymocytes and bone marrow cells were also AVM-1 negative. Anti-AVM-1 monoclonal antibody immunoprecipitated a single polypeptide with a molecular weight of 200,000. Of particular interest was the finding that the anti-AVM-1 antibody could inhibit the formation of EA and EAC rosette on macrophage line cells. A second antigen (AVM-2) was also present on alveolar macrophages, and its molecular weight was 38,000.
Yoshikawa
K
K
Department of Microbiology, Aichi Medical University School of Medicine, Japan.
Suzuki
Y
Y
Kawai
M
M
Fukada
M
M
Yokochi
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D002462:Cell Membrane / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D011233:Precipitin Tests; D011650:Pulmonary Alveoli / Q000166:cytology* / Q000276:immunology; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D008565:Membrane Proteins