A specific, sensitive and high-capacity immunoassay for PAF.
A specific radioimmunoassay for platelet-activating factor (PAF) sensitive in the range 10-1000 pg (0.02-2 pmoles) has been developed. Detailed quantitative hapten inhibition studies showed specificity for the acetyl group at C-2 of PAF, a requirement for the ether linkage at C-1 and some tolerance for substituents on the choline nitrogen. No significant cross-reactivity was found with phosphatidylcholine and lysophosphatidylcholine or with lysoPAF.
Baldo
B A
BA
Kolling Institute of Medical Research, Royal North Shore Hospital of Sydney, St. Leonards, N.S.W., Australia.
Smal
M A
MA
McCaskill
A C
AC
D000818:Animals; D001681:Biological Assay / Q000379:methods; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology; D003429:Cross Reactions; D006241:Haptens; D006801:Humans; D008832:Microchemistry; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000032:analysis* / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects; D011863:Radioimmunoassay / Q000379:methods; D011869:Radioligand Assay / Q000379:methods; D012756:Sheep / Q000276:immunology; D013329:Structure-Activity Relationship
D006241:Haptens; D010972:Platelet Activating Factor