title: Activation of hyaluronidase by 8-methoxypsoralen-polyamine photoproducts.
abstract: Ultraviolet radiation of 8-methoxypsoralen (8-MOP) in the presence of various polyamines resulted in stable photoproducts that were very soluble in water and showed hyaluronidase-activating properties. Among them, the photoproducts obtained from the reaction systems of 8-MOP-spermine and 8-MOP-spermidine markedly activated hyaluronidase. The enzyme activity was not affected by 8-MOP alone and the photoproduct of 8-MOP (8-MOP-P). From these facts, it was suggested that the photoproducts with hyaluronidase-activating properties might play an important role in the onset of 8-MOP-induced photosensitivity.
authors.affiliation: Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Shigehara
authors.forename: H
authors.initials: H
authors.lastname: Kakegawa
authors.forename: H
authors.initials: H
authors.lastname: Miyataka
authors.forename: H
authors.initials: H
authors.lastname: Matsumoto
authors.forename: A
authors.initials: A
authors.lastname: Isobe
authors.forename: T
authors.initials: T
authors.lastname: Satoh
mesh_terms: D000818:Animals; D002417:Cattle; D002855:Chromatography, Thin Layer; D004789:Enzyme Activation / Q000187:drug effects; D006821:Hyaluronoglucosaminidase / Q000737:chemistry*; D008730:Methoxsalen / Q000494:pharmacology / Q000528:radiation effects*; D011073:Polyamines / Q000494:pharmacology / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D011073:Polyamines; D006821:Hyaluronoglucosaminidase; D008730:Methoxsalen