title: Cellular damage and early metabolic function of transplanted livers stored in Eurocollins or University of Wisconsin solution.
abstract: In a clinical setting, the effect of Eurocollins (EC) and University of Wisconsin solution (UW) on liver grafts were studied in the early reperfusion phase of liver transplantation. Blood samples were drawn before and after declamping of the portal vein in a group of 11 transplants with EC-perfused livers, and a group of 12 transplants with UW-perfused livers. Parenchymal damage was assessed by the LDH, AST, and ALT, and purine degradation by measuring the uric acid levels. Metabolic function was determined by the serum bile acids and the plasma amino acids, i.e. (valine + leucine + isoleucine)/(phenylalanine + tyrosine) ratio. Donor and pretransplant recipient parameters were almost identical. The cold ischemia time of both groups differed significantly. The results show the following: a significant difference between both the LDH and the uric acid levels in the two groups was revealed, with a smaller increase of the LDH levels and no increase of the uric acid levels in the UW group. Metabolic activity, as measured from the bile acids and the amino acid profile in the peripheral blood, was identical in both groups. We conclude that both EC-stored and UW-stored liver grafts show immediate metabolic function after reperfusion. The amount of metabolic function was equal in both groups, notwithstanding longer cold ischemia time in the UW group. In addition, more parenchymal damage occurred in the EC group.
authors.affiliation: Department of Surgery, University Hospital Groningen, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Pruim
authors.forename: E M
authors.initials: EM
authors.lastname: ten Vergert
authors.forename: I J
authors.initials: IJ
authors.lastname: Klompmaker
authors.forename: R
authors.initials: R
authors.lastname: Verwer
authors.forename: M J
authors.initials: MJ
authors.lastname: Slooff
mesh_terms: D000241:Adenosine; D000328:Adult; D000493:Allopurinol; D000596:Amino Acids / Q000378:metabolism; D001647:Bile Acids and Salts / Q000378:metabolism; D005260:Female; D005978:Glutathione; D006801:Humans; D006982:Hypertonic Solutions / Q000494:pharmacology*; D007328:Insulin; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D011887:Raffinose; D012996:Solutions / Q000494:pharmacology*; D014527:Uric Acid / Q000378:metabolism
chemical_list: D000596:Amino Acids; D001647:Bile Acids and Salts; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D014527:Uric Acid; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose