Does Convit vaccine (BCG + Mycobacterium leprae) afford protection against biochemical changes in renal brush border membrane in experimental leprosy?
Renal functional status in Mycobacterium leprae infected mice can be best studied by examining the enzymatic status of brush border membrane vesicles from proximal convoluted tubule. The role of vaccination in modulation of the renal status brought by the disease has been studied using this technique. The characteristic marker enzymes of renal brush border membrane--namely alkaline phosphatase, leucine aminopeptidase and gamma-glutamyl transpeptidase decreased significantly (p less than 0.01) in due course in M. leprae infection over a period of 9 months. The combined vaccine (BCG + M. leprae) may have a protective effect on renal abnormalities only in the initial stages of infection as indicated by a significant rise in enzymatic levels. However, no significant (p greater than 0.05) protective effect of vaccine was found in a more advanced disease state after 9 months in infected mice.
Kohli
M
M
Department of Dermatology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Ganguly
N K
NK
Kaur
S
S
Chugh
K S
KS
Sharma
V K
VK
Bhushnurmath
S R
SR
D000818:Animals; D001500:BCG Vaccine / Q000008:administration & dosage*; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D007672:Kidney Cortex / Q000201:enzymology*; D007918:Leprosy / Q000201:enzymology* / Q000517:prevention & control; D051379:Mice; D008871:Microvilli / Q000201:enzymology; D009166:Mycobacterium leprae / Q000276:immunology
D001500:BCG Vaccine; D001428:Bacterial Vaccines; C060596:anti-leprosy vaccine