title: Differential scanning calorimetry characterization of rabbit brain membrane fractions.
abstract: Various membrane fractions have been prepared from rabbit brain synaptosomes by centrifugation on discontinuous sucrose gradients after osmotic shock. These fractions were characterized by electron microscopy (E.M.), SDS-PAGE and GABA binding. The fractions were then studied by differential scanning calorimetry (DSC). The calorimetric results indicate that all the fractions studied show thermal transitions at around 60 degrees C which correspond to the "melting" of membrane structures. An additional transition at higher temperature (82 degrees C) seems to be associated with an enrichment in central myelin fragments.
authors.affiliation: Centro di Neurofisiologia Cerebrale, C.N.R., Genova, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Cupello
authors.forename: G L
authors.initials: GL
authors.lastname: Mancardi
authors.forename: G
authors.initials: G
authors.lastname: Candiano
authors.forename: G
authors.initials: G
authors.lastname: Rialdi
mesh_terms: D000818:Animals; D001921:Brain / Q000648:ultrastructure; D001923:Brain Chemistry / Q000502:physiology*; D002152:Calorimetry, Differential Scanning; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D008297:Male; D008566:Membranes / Q000737:chemistry / Q000648:ultrastructure; D009186:Myelin Sheath / Q000737:chemistry; D009419:Nerve Tissue Proteins / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D011817:Rabbits; D013347:Subcellular Fractions / Q000737:chemistry / Q000648:ultrastructure; D013395:Sucrose; D013574:Synaptosomes / Q000737:chemistry; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D009419:Nerve Tissue Proteins; D010455:Peptides; D005680:gamma-Aminobutyric Acid; D013395:Sucrose