Rapid electrodialytic clean-up of biological samples for high-performance liquid chromatography.
A sample clean-up system employing electrodialysis with size-selective and charge-selective membranes is described. When applied to the treatment of 0.5-ml milk samples containing sulfamethazine, the system produced an undiluted, clear solution in 3 min and eliminated the components in untreated milk that caused column fouling and double peaks. In contrast to conventional liquid- and solid-phase extraction procedures, electrodialytic clean-up is readily automated and uses no organic solvents.
Brewster
J D
JD
US Department of Agriculture, ARS, Eastern Regional Research Center, Philadelphia, PA 19118.
Piotrowski
E G
EG
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003956:Dialysis / Q000379:methods*; D004563:Electrochemistry; D008892:Milk / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D013418:Sulfamethazine / Q000032:analysis
D013418:Sulfamethazine