Simple isocratic high-performance liquid chromatographic method for measurement of iodixanol in human plasma.
A simple isocratic high-performance liquid chromatographic method was developed for the determination of iodixanol in human plasma. Samples containing an internal standard were prepared for analysis using a simple clean-up procedure based on Sep-Pak C18 solid-phase extraction and chromatographed using a size-exclusion column with purified water as a mobile phase. The iodixanol peak was completely separated from the peaks of an internal standard and endogenous substances on this column. Three geometric isomers (exo-exo, endo-exo and endo-endo forms) of iodixanol could be eluted as a single peak. The method was found to be applicable to pharmacokinetic studies of iodixanol in human plasma.
Nomura
H
H
Drug Metabolism and Analytical Chemistry Research Center, Daiichi Pharmaceutical Co., Ltd., Tokyo, Japan.
Teshima
E
E
Hakusui
H
H
D002851:Chromatography, High Pressure Liquid; D003287:Contrast Media / Q000378:metabolism*; D006801:Humans; D008297:Male; D012016:Reference Values; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014283:Triiodobenzoic Acids / Q000097:blood*
D003287:Contrast Media; D014283:Triiodobenzoic Acids; C044834:iodixanol