Synthesis of a [1,2-3H]-labeled pregnanolone.
A method is described for the synthesis and purification of 3 alpha-hydroxy-5 alpha-[1,2-3H]pregnan-20-one. [1,2-3H]progesterone (55 Ci/mmol) was incubated with a homogenate of rat brain tissue. The product was purified by Sephadex chromatography and thin-layer chromatography. The identity and purity of the product were established by successive recrystallizations and high-performance liquid chromatography. A 34% portion of the starting material was converted to 3 alpha-hydroxy-5 alpha-[1,2-3H]pregnan-20-one. The final radiopurity of 3 alpha-hydroxy-5 alpha-pregnan-20-one obtained from four independent preparations was 94% to 99%.
Mok
W M
WM
Department of Anesthesia, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts 02115.
Krieger
N R
NR
D000777:Anesthetics / Q000138:chemical synthesis* / Q000302:isolation & purification; D000818:Animals; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D008297:Male; D015394:Molecular Structure; D011280:Pregnanolone / Q000138:chemical synthesis* / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D012015:Reference Standards; D014316:Tritium
D000777:Anesthetics; D014316:Tritium; D011280:Pregnanolone