[New perspectives of in vitro kallikrein treatment of human spermatozoa. I. Study of the hamster ovum penetration test in relation to different andrologic patient groups].
Whereas the enhancement of motility by Kallikrein is described several times, the effect on fertilizing capacity of human spermatozoa has not been investigated sufficiently yet. This fact induced the authors to start a further investigation on 64 patients by the means of zona-free hamster oocytes. The patients were differentiated by the parameters density and motility. The sample was divided up into two aliquots. Differing from the control the other aliquot was prepared with Kallikrein (5 KU/ml) before swim-up and preincubation for one hour at 22 degrees C. Comparing the results we found looking at the whole collective (n = 64) a significant enhancement of the fertilizing capacity. 34 of 64 patients showed an improvement, only 4 patients deteriorated. While the groups normo-, oligo-, and asthenozoospermia reached a higher fertilizing capacity under Kallikrein-treatment (probability of error 2.5%), a Kallikrein application in case of oligo-asthenozoospermia had no significant influence on fertilizing capacity. A view of the whole results leads to the assumption of a direct effect of Kallikrein and to the possibility of use for GIFT and IVF procedure.
Sterzik
K
K
Frauenklinik Universität Ulm.
Strehler
E
E
Schmid
U
U
Rosenbusch
B
B
Schill
W B
WB
Lauritzen
C
C
D000328:Adult; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007610:Kallikreins / Q000008:administration & dosage*; D008297:Male; D013081:Sperm Motility / Q000187:drug effects*; D013084:Sperm-Ovum Interactions / Q000187:drug effects*
D007610:Kallikreins
Neue Perspektiven der In-vitro-Kallikrein-Behandlung menschlicher Spermatozoen. I. Untersuchung im Hamsterovum-Penetrationstest in Abhängigkeit von unterschied-lichen andrologischen Patientengruppen.