Vasopressin-induced changes in receptor-mediated endocytosis of asialoglycoprotein in rat hepatocytes.
The ability of second messengers to modulate receptor-mediated endocytosis was studied on isolated rat hepatocytes. A 20-min preincubation with vasopressin was used as a modulation. We observed a 20% inactivation of both surface and intracellular receptors, with no change in the affinity of those remaining active. The internalization and dissociation of a synchronous wave of ligand was not affected, but its degradation was partially inhibited. Our observations suggest that second messengers such as intracellular calcium and diacylglycerol play a complex role in the intracellular trafficking associated with endocytosis.
Gil-Falgon
S
S
Laboratoire de Biochimie, UFR des Sciences Pharmaceutiques et Biologiques, université Paris-Sud, Châtenay-Malabry, France.
Lamaze
C
C
Feger
J
J
D000818:Animals; D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins / Q000378:metabolism*; D004705:Endocytosis / Q000187:drug effects; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011971:Receptors, Immunologic / Q000378:metabolism*; D015290:Second Messenger Systems; D014667:Vasopressins / Q000494:pharmacology*
D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D011971:Receptors, Immunologic; D014667:Vasopressins