[Intraocular availability of liposome encapsulated monoclonal antibodies in the rabbit model. Results of a pilot study].
The results of local application of monoclonal antibodies (mAb) in rabbit eyes are presented. To improve intraocular access of the high-molecular-weight protein it was entrapped in large (0.2 microns) unilamellar, negatively charged liposomes. Concentrations of the free or encapsulated drug were measured by ELISA in different eye compartments following repetitive drop administration or single subconjunctival injection. Although mAb became measurable in specimens of conjunctiva and cornea, it was not detectable (less than 0.5 ng/ml) in aqueous humor, lens or the vitreous body. In contrast, concentrations of the liposome-encapsulated drug were measurable as little as 30 min after topical application in the aqueous humor.
Pleyer
U
U
Universitäts-Augenklinik, Tübingen, Bundesrepublik Deutschland.
Rückert
D
D
Bachmann
W
W
Schmidt
K H
KH
Thiel
H J
HJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000378:metabolism*; D001682:Biological Availability; D004337:Drug Carriers; D005123:Eye / Q000187:drug effects* / Q000378:metabolism*; D005614:Freeze Fracturing; D008081:Liposomes; D008854:Microscopy, Electron; D011817:Rabbits
D000911:Antibodies, Monoclonal; D004337:Drug Carriers; D008081:Liposomes
Intraokulare Verfügbarkeit liposomenverkapselter monoklonaler Antikörper im Kaninchenmodell. Ergebnisse einer Pilotstudie.