[Isolation and characteristics of vaccinia virus proteins associated with cell membranes of infected cells].
The proteins of vaccinia virus associated with plasma membrane infected cells BHK-21, p60, p45, p42, p40,p35,p34,p28,p23 were isolated from plasma membranes using affinity chromatography, gel-electrophoresis and passive elution. An immunochemical characterization was carried out using specific antiserum to these proteins. Investigation of temporal regulation of proteins synthesis in infected cells showed that proteins p60, p45, p42, p40, p28 were late, and p35, p34, p23--early-late proteins. Immunochemical analysis of vaccinia virus mRNA cell-free translational products was carried out using specific antiserum. The polypeptide-precursors of viral proteins were identified.
Cheshenko
N V
NV
Netesova
N A
NA
Malygin
E G
EG
D000818:Animals; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism / Q000382:microbiology; D002478:Cells, Cultured; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014616:Vaccinia virus / Q000378:metabolism*; D014764:Viral Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism
D007106:Immune Sera; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins
Vydelenie i kharakteristika belkov virusa ospovaktsiny, assotsirovannykh s plazmaticheskimi membranami infitsirovannykh kletok.