Optical separation of racemic 5-(p-hydroxyphenyl)-5-phenylhydantoin by reversed phase high-performance liquid chromatography using eluents containing beta-cyclodextrin.
Both S-(-)- and R-(+)-enantiomers of 5-(p-hydroxyphenyl)-5-phenylhydantoin (p-HPPH), a main oxidative metabolite of the achiral antiepileptic drug phenytoin, could be determined simply, sensitively and accurately using reversed phase high-performance liquid chromatography by using a methanol-monopotassium phosphate eluent containing beta-cyclodextrin. Using this assay procedure, it was determined that an S-(-)-enantiomer was formed predominantly by the oxidation of phenytoin in isolated rat hepatocytes.
Eto
S
S
Department of Hospital Pharmacy, School of Medicine, University of Occupational and Environmental Health, Japan.
Noda
H
H
Minemoto
M
M
Noda
A
A
Mizukami
Y
Y
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000737:chemistry*; D008099:Liver / Q000737:chemistry / Q000166:cytology; D010672:Phenytoin / Q000031:analogs & derivatives* / Q000302:isolation & purification; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D003505:Cyclodextrins; C027364:5-(3-hydroxyphenyl)-5-phenylhydantoin; D010672:Phenytoin