Detection and purification of antiidiotypic antibody against anti-DNA in intravenous immune globulin.
Pooled normal human IgG for therapeutic use, following depletion of anti-DNA, anti-Fc, and anti-F(ab')2 of normal IgG, expressed antiidiotypic activity against anti-DNA derived from lupus sera. The antiidiotype enriched by elution from anti-DNA affinity columns bound directly to anti-DNA IgG and inhibited the binding of lupus sera to DNA but did not bind to normal IgG or inhibit the binding of anti-tetanus toxoid to tetanus toxoid. Antiidiotypes in pooled normal sera may have a role in the clinical improvement seen in patients with autoimmune diseases receiving intravenous immune globulin.
Evans
M J
MJ
Evans Immunology Research Laboratory, St. Luke's Hospital, Kansas City 64111.
Suenaga
R
R
Abdou
N I
NI
D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification*; D000974:Antibodies, Antinuclear / Q000302:isolation & purification*; D000918:Antibody Specificity / Q000276:immunology; D001667:Binding, Competitive / Q000276:immunology; D002846:Chromatography, Affinity; D004247:DNA / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D016756:Immunoglobulins, Intravenous / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000974:Antibodies, Antinuclear; D007074:Immunoglobulin G; D016756:Immunoglobulins, Intravenous; D004247:DNA