Enzyme negative blastic transformation of chronic myeloproliferative disorders: immunophenotyping of the blastic cell population.
Enzyme negative blast cells from 27 patients with chronic myeloproliferative disorders (CMPDs) in blastic transformation were analysed with a panel of monoclonal antibodies (MoAbs). According to morphologic features of the bone marrow and laboratory data, the 27 cases were divided into 8 cases of myelofibrosis (MF), 3 cases of chronic megakaryocytic granulocytic myelosis (CMGM) and 16 cases of chronic myeloid leukaemia (CML). Of the 27 cases, 23 showed a positive reaction with myeloid MoAbs, but in 12 cases expressing myeloid markers, megakaryocytic, monocytic or lymphoid cell features were also detected. In 7 cases of MF, 1 case of CMGM and 1 case of CML a bilineage, myelo-megakaryocytoid immunophenotype of peripheral blast cells was seen. Of the 4 patients with CML expressing lymphoid markers, 2 showed early B-cell, 1 T-cell surface antigens, and 1 both myeloid and early B-cell features. In this group of cytochemically immature blastic transformation of CMPD, only 1 case was termed "undifferentiated" blastic transformation.
Matolcsy
A
A
Department of Pathology, University Medical School of Pécs, Hungary.
Izer
I
I
Magyarlaki
T
T
Baranyay
F
F
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001752:Blast Crisis / Q000201:enzymology / Q000276:immunology*; D002908:Chronic Disease; D006801:Humans; D016130:Immunophenotyping; D008875:Middle Aged; D009196:Myeloproliferative Disorders / Q000473:pathology*
D000911:Antibodies, Monoclonal