title: Quantitative analysis of erythromycin by reversed-phase liquid chromatography using column-switching.
abstract: A column-switching technique is described for LC of erythromycin. The method allows, in about 1 h, the separation of erythromycin A from all its known potential impurities, except erythromycin D, which is a minor impurity. The switching technique combines two columns (7.5 cm x 4.6 mm and 25.0 cm x 4.6 mm) both packed with RSil C 18 LL 10 microns. The mobile phase is acetonitrile-tetrabutylammonium sulphate (0.2 M, pH 6.0)-ammonium phosphate buffer (0.2 M, pH 6.0)-water (24:5:5:66, v/v/v/v). Temperature was 35 degrees C, flow rate was 1.5 ml min-1, detection was by UV at 210 nm. Results for a number of commercial samples of various origin are reported.
authors.affiliation: Katholieke Universiteit Leuven, Instituut voor Farmaceutische Wetenschappen, Belgium.
authors.forename: T
authors.initials: T
authors.lastname: Cachet
authors.forename: K
authors.initials: K
authors.lastname: de Turck
authors.forename: E
authors.initials: E
authors.lastname: Roets
authors.forename: J
authors.initials: J
authors.lastname: Hoogmartens
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D002021:Buffers; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004917:Erythromycin / Q000031:analogs & derivatives / Q000032:analysis*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013696:Temperature
chemical_list: D000097:Acetonitriles; D002021:Buffers; D000644:Quaternary Ammonium Compounds; D004917:Erythromycin; C009405:tetrabutylammonium; C032159:acetonitrile