[Use of the DNA-specific fluorochrome olivomycin for work with cell cultures].
A high-sensitive, easy and rapid proximate method has been developed to reveal mycoplasmas in the monolayer cell cultures using home fluorescent antibiotic olivomycin. This method has been used to screen many cell lines with its high effectiveness being shown. As based on this method the following control methods are worked out: indication of mycoplasmas in animal blood sera, used for the cultivation of cell lines detection of contaminant microorganisms of the cell cultures (fungi and bacteria) and human ureaplasmas.
Mikhaĭlova
G R
GR
Berdnikova
Z E
ZE
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification; D002478:Cells, Cultured; D004247:DNA / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D005658:Fungi / Q000302:isolation & purification; D006801:Humans; D009174:Mycoplasma / Q000302:isolation & purification*; D009848:Olivomycins / Q000494:pharmacology*; D013045:Species Specificity; D014509:Ureaplasma / Q000302:isolation & purification
D009848:Olivomycins; D004247:DNA
Ispol'zovanie DNK-spetsifichnogo fliuorokhroma olivomitsina dlia raboty s kletochnymi kul'turami.