title: A competitive radioimmunoassay for peptidoglycan monomer.
abstract: The preparation and characterization of the essential components to be used in the radioimmunoassay of peptidoglycan monomer (PGM) is described. In order to raise the anti-peptidoglycan monomer antibodies 14C-labelled peptidoglycan monomer-bovine serum albumin conjugate was prepared by the coupling of 14C-peptidoglycan monomer to bovine serum albumin in the presence of glutaraldehyde in 0.1 M NaHCO3 at pH 8.3. The prepared conjugate elicited anti-PGM response in rabbits. A synthetic analog of peptidoglycan monomer, Boc-L-tyrosyl-peptidoglycan monomer was prepared by condensation of unprotected peptidoglycan monomer and N-hydroxysuccinimidester of Boc-L-tyrosine in the presence of triethylamine and the obtained disaccharide-hexapeptide was labelled with Na125I. This compound exhibited the ability of binding to anti-peptidoglycan monomer antibodies. The prepared compounds, namely anti-PGM antibodies and 125I-labelled Boc-L-tyrosyl-peptidoglycan monomer, were used as essential components in competitive radioimmunoassay for peptidoglycan monomer determination in mammalian and human sera and plasma, respectively.
authors.affiliation: Department of Radioimmunology, Institute of Immunology, Zagreb, Croatia, Yugoslavia.
authors.forename: B
authors.initials: B
authors.lastname: Vranesić
authors.forename: D
authors.initials: D
authors.lastname: Ljevaković
authors.forename: J
authors.initials: J
authors.lastname: Tomasić
authors.forename: B
authors.initials: B
authors.lastname: Ladesić
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies; D001951:Brevibacterium; D002240:Carbohydrate Sequence; D002250:Carbon Radioisotopes; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D004590:Electrophoresis, Paper / Q000379:methods; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000032:analysis* / Q000302:isolation & purification; D011863:Radioimmunoassay / Q000379:methods
chemical_list: D000906:Antibodies; D002250:Carbon Radioisotopes; D010457:Peptidoglycan