Expression of cauliflower mosaic virus gene I in Saccharomyces cerevisiae.
Cauliflower mosaic virus (CaMV) gene I encodes a 40-kDa protein, P1, which is thought to be involved in the cell-to-cell movement of the virus. In order to investigate its functioning, P1 was expressed in Saccharomyces cerevisiae transformed by an expression vector containing CaMV gene I. When produced in yeast, PI was 40 kDa in size and not N-glycosylated.
Kirchherr
D
D
Instit de Biologie moléculaire des Plantes du CNRS, Strasbourg, France.
Wurch
T
T
Mesnard
J M
JM
Lebeurier
G
G
D015153:Blotting, Western; D003001:Cloning, Molecular; D015870:Gene Expression / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D006031:Glycosylation; D009029:Mosaic Viruses / Q000235:genetics*; D010945:Plants, Edible / Q000382:microbiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
C073233:P1 protein, Cauliflower mosaic virus; D014764:Viral Proteins