Clomipramine actions on firing rate in septal nuclei of the rat are not related to anaesthesia (urethane).
An increased firing rate in lateral septal nuclei (LSN) appears in urethane-anesthetized rats after several acute drug and non-drug human antidepressant treatments. A still more pronounced increase in firing rate is produced in LSN after clomipramine (CMI) long-term treatment. In spite of urethane is a widely used anesthetic for single unit extracellular recordings, it modifies evoked potentials wave-form. Therefore, present study discards urethane interaction with CMI in LSN single unit extracellular recordings. CMI was acutely injected (1.25 mg/kg: IP) either to urethane-anesthetized, or non-anesthetized encephale-isolé rats. The CMI treated groups showed higher rates of firing in LSN regardless of the use of general anesthesia during recordings. Another group of urethane-anesthetized rats received intracerebroventricular (ICV) microinjections of CMI (100 micrograms/10 microliters/1 min). An amount of 42.8% of LSN-recorded neurons responded with a long-lasting increased firing rate. Results discard urethane and CMI interactions. Additionally, systemic actions of CMI on firing rate of LSN are reproduced by ICV/route microinjections.
Contreras
C M
CM
Departamento de Fisiología, Instituto de Investigaciones Biomédicas, U.N.A.M., México, D.F.
Marván
M L
ML
Lara-Morales
H
H
Barradas
A
A
Chacón
L
L
Guzmán-Sáenz
M A
MA
Márquez Flores
C
C
D000200:Action Potentials / Q000187:drug effects*; D000818:Animals; D002997:Clomipramine / Q000494:pharmacology*; D003655:Decerebrate State; D008012:Lidocaine / Q000494:pharmacology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012686:Septal Nuclei / Q000187:drug effects* / Q000502:physiology; D012701:Serotonin / Q000378:metabolism; D014520:Urethane / Q000494:pharmacology*
D012701:Serotonin; D014520:Urethane; D008012:Lidocaine; D002997:Clomipramine