Arsenite as the probable active species in the human carcinogenicity of arsenic: mouse micronucleus assays on Na and K arsenite, orpiment, and Fowler's solution.
Sodium arsenite, potassium arsenite, and Fowler's solution (arsenic trioxide dissolved in potassium bicarbonate) are equally active in the mouse bone marrow micronucleus assay (approximately 10 mg/kg by IP injection). The natural ore orpiment (principally As2S3) was inactive despite blood levels of arsenic of 300 to 900 ng/mL in treated mice at 24 hr. Sodium arsenite was active in three strains of mice. It is suggested that the human lung cancer observed among arsenic ore smelters and the skin cancer among people exposed therapeutically to Fowler's solution, have, as their common origin, the genotoxic arsenite ion AsO2-. The difficulty experienced when attempting to demonstrate rodent carcinogenicity for derivatives of arsenic suggests that the bone marrow micronucleus assay may act as a useful assay for potentially carcinogenic arsenic derivatives.
Tinwell
H
H
ICI Central Toxicology Laboratory, Alderly Park, Cheshire, U.K.
Stephens
S C
SC
Ashby
J
J
D000818:Animals; D001151:Arsenic / Q000009:adverse effects / Q000097:blood*; D001152:Arsenicals; D018053:Arsenites; D001856:Bone Marrow Examination; D008175:Lung Neoplasms / Q000139:chemically induced; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D015162:Micronucleus Tests; D011188:Potassium / Q000009:adverse effects / Q000097:blood; D017680:Potassium Compounds; D012878:Skin Neoplasms / Q000139:chemically induced; D017670:Sodium Compounds; D013440:Sulfides / Q000009:adverse effects / Q000097:blood
D001152:Arsenicals; D018053:Arsenites; D017680:Potassium Compounds; D017670:Sodium Compounds; D013440:Sulfides; C009003:potassium arsenite solution; C045816:arsenic trisulfide; C017947:sodium arsenite; D001151:Arsenic; D011188:Potassium