Platelet activating factor-induced pulmonary accumulation of 111Indium-oxine labelled neutrophils in anesthetized guinea pigs.
The thoracic accumulation of neutrophils labelled with 111Indium-oxine in response to infusion of platelet activating factor (PAF, 18 ng/kg/min x 5 min, i.v.) was studied using an automated isotope monitoring system (AIMSplus) in anesthetized guinea-pigs. Loss of cell associated radioactivity in vitro was less than 1% over 4 hr. Labelled neutrophils maintained their functional capacity (oxidative response to the cell stimulants N-formyl-L-methionine-L-leucine-L-phenylalanine and phorbol myristate acetate) and greater than 95% viability (ethidium bromide/acridine orange stain) in vitro. Total thoracic radioactivity increased significantly from baseline in response to PAF with a slight tachyphylaxia in the neutrophil-accumulation after a repeat PAF infusion. The highest ratios of radiolabel (tissue/blood) were found in the spleen much greater than liver greater than lung.
Hultkvist-Bengtsson
U
U
Preclinical Research, Sandoz AG, Basel, Switzerland.
Anderson
G P
GP
Kings
M
M
Morley
J
J
D000704:Analysis of Variance; D000768:Anesthesia, General; D000818:Animals; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007205:Indium Radioisotopes; D007700:Kinetics; D008168:Lung / Q000187:drug effects / Q000502:physiology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D009942:Organometallic Compounds; D015125:Oxyquinoline / Q000031:analogs & derivatives; D010972:Platelet Activating Factor / Q000494:pharmacology*; D011865:Radioisotope Dilution Technique; D013755:Tetradecanoylphorbol Acetate
D007205:Indium Radioisotopes; D009942:Organometallic Compounds; D010972:Platelet Activating Factor; C032950:indium oxine; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015125:Oxyquinoline; D013755:Tetradecanoylphorbol Acetate