title: Purification and analysis of growth-regulating xyloglucan-derived oligosaccharides by high-pressure liquid chromatography.
abstract: The retention times of 10 oligosaccharides, generated from the xyloglucans of Rosa and Tropaeolum by the action of Trichoderma cellulase, and of 17 related carbohydrates, in h.p.l.c. on an amino-substituted silica (Amino-Spheri-5) depended largely on the number of hydroxyl groups per molecule, whereas h.p.l.c. on a pellicular anion-exchange resin (CarboPac PA1) was strongly influenced by the nature of the sugar residues present, especially L-fucose, and by their linkages. The major nonasaccharide (XG9, D-Glc4-D-Xyl3-D-Gal-L-Fuc) obtained from Rosa xyloglucan, after purification on Amino-Spheri-5, retained biological activity as an inhibitor of auxin-induced growth in a Pisum stem-segment bioassay. H.p.l.c. on Amino-Spheri-5 was used to monitor the action of "Driselase" in stripping the non-reducing terminal alpha-D-Xylp-(1----6)-beta-D-Glcp units from XG9 to yield a pentasaccharide (XG5, D-Glc2-D-Xyl-D-Gal-L-Fuc).
authors.affiliation: Department of Botany, University of Edinburgh, Great Britain.
authors.forename: G J
authors.initials: GJ
authors.lastname: McDougall
authors.forename: S C
authors.initials: SC
authors.lastname: Fry
mesh_terms: D001681:Biological Assay; D002240:Carbohydrate Sequence; D002480:Cellulase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D007887:Fabaceae / Q000378:metabolism; D005936:Glucans; D007210:Indoleacetic Acids; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification* / Q000378:metabolism; D063245:Plant Development; D010944:Plants / Q000737:chemistry* / Q000378:metabolism; D010946:Plants, Medicinal; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology; D014990:Xylans
chemical_list: D005936:Glucans; D007210:Indoleacetic Acids; D009844:Oligosaccharides; D011134:Polysaccharides; D014990:Xylans; C029353:xyloglucan; D002480:Cellulase