Crystallization and preliminary diffraction analysis of caudoxin and notexin; two monomeric phospholipase A2 neurotoxins.
Two monomeric neurotoxic phospholipases A2 have been crystallized and their diffraction properties characterized. Crystals of caudoxin (from the venom of Bitis caudalis) and notexin (from the venom of Notechis scutatus scutatus) were grown at neutral pH, in the absence of calcium ion, and diffract to a resolution of 2.3 and 1.6 A, respectively.
Scott
D L
DL
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
Achari
A
A
Christensen
P A
PA
Viljoen
C C
CC
Sigler
P B
PB
D002118:Calcium / Q000737:chemistry; D003460:Crystallization; D004546:Elapid Venoms / Q000737:chemistry*; D054501:Group II Phospholipases A2; D010741:Phospholipases A / Q000737:chemistry*; D054467:Phospholipases A2; D030162:Reptilian Proteins; D014757:Viper Venoms / Q000737:chemistry*; D014961:X-Ray Diffraction
D004546:Elapid Venoms; D030162:Reptilian Proteins; D014757:Viper Venoms; C005082:notexin; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054467:Phospholipases A2; C034914:caudoxin; D002118:Calcium