title: Collisional spectroscopy approaches in the characterization of 7-alpha- and 7-beta-hydroxycholesterol.
abstract: High- and low-energy collision experiments, by means of double-focusing and ion-trap mass spectrometers, respectively, performed on the M+ and [M-H2O]+. ions of 7-alpha- and 7-beta-hydroxycholesterol lead to a clear structural characterization of the two stereoisomers. In particular, the beta isomer shows peculiar fragmentation pathways due to interaction between the hydroxyl group in position 7-beta and the hydrogen atom on C(15).
authors.affiliation: CNR, Padova, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Favretto
authors.forename: F
authors.initials: F
authors.lastname: Guidugli
authors.forename: R
authors.initials: R
authors.lastname: Seraglia
authors.forename: P
authors.initials: P
authors.lastname: Traldi
authors.forename: F
authors.initials: F
authors.lastname: Ursini
authors.forename: A
authors.initials: A
authors.lastname: Sevanian
mesh_terms: D006888:Hydroxycholesterols; D013058:Mass Spectrometry / Q000379:methods*; D013237:Stereoisomerism
chemical_list: D006888:Hydroxycholesterols