title: The structure of murine interleukin-1 beta at 2.8 A resolution.
abstract: The three-dimensional structure of recombinant murine interleukin-1 beta has been solved by X-ray crystallographic techniques to 2.8 A resolution and refined to a crystallographic R factor of 0.192. Although murine interleukin-1 beta crystallizes in the same space group as human interleukin-1 beta with almost identical unit cell dimensions, the packing of the molecules is quite different. The murine interleukin-1 beta structure was solved by molecular replacement using the refined structure of human interleukin-1 beta as trial structure, and found to be related to the human structure by a nearly perfect twofold rotation about the crystallographic y-axis and a 14 degrees rotation about the z-axis, with no translation. The folding of murine interleukin-1 beta is similar to that found for the human variant, consisting of 12 beta strands wrapped around a core of hydrophobic side chains in a tetrahedron-like fashion. Significant differences with respect to the human structure are seen at the N terminus and in 4 of the 11 loops connecting the 12 beta strands.
authors.affiliation: Department of Biotechnology, Ciba-Geigy Ltd., Basel, Switzerland.
authors.forename: J
authors.initials: J
authors.lastname: van Oostrum
authors.forename: J P
authors.initials: JP
authors.lastname: Priestle
authors.forename: M G
authors.initials: MG
authors.lastname: Grütter
authors.forename: A
authors.initials: A
authors.lastname: Schmitz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003198:Computer Simulation; D003460:Crystallization; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007375:Interleukin-1 / Q000737:chemistry*; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016415:Sequence Alignment; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D007375:Interleukin-1