A scaffold-associated DNA region is located downstream of the pea plastocyanin gene.
Chromosomal scaffold-associated DNA has been isolated from pea leaf nuclei treated with lithium diiodosalicylate to remove histones and then digested with restriction enzymes to remove the DNA in chromosomal loops. A scaffold-associated region (SAR) of DNA has been identified 8 to 9 kb downstream of the single-copy pea plastocyanin gene in proximity to a repetitive sequence present in 300 copies in the pea haploid genome. Isolated restriction fragments from within the SAR can bind to scaffold preparations in a binding assay in vitro. The nucleotide sequence of the SAR indicates a 540-bp 77% A+T-rich region containing many sequence elements in common with SARs from other organisms. Sequences with homology to topoisomerase II binding sites, A-box and T-box sequences, and replication origins are present within this AT-rich region.
Slatter
R E
RE
Botany School, University of Cambridge, United Kingdom.
Dupree
P
P
Gray
J C
JC
D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D007887:Fabaceae / Q000235:genetics; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics*; D010946:Plants, Medicinal; D010970:Plastocyanin / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D004247:DNA; D010970:Plastocyanin