Translocation of heat shock proteins into isolated nuclei of Aedes albopictus cells.
1. Labelled heat shock proteins of 82 kDa, 70 kDa and a group of low molecular weight are present in the post-mitochondrial supernatant obtained from Aedes albopictus cells. 2. These proteins in the post-mitochondrial extract are transported specifically to the nuclear compartment when incubated with isolated unlabelled nuclei. The relative amounts of each protein transported were 4.7, 24.1 and 3.1% for the 82-kDa and 70-kDa and for the low molecular weight proteins, respectively. 3. Incubation of radiolabelled post-mitochondrial extract with unlabelled nuclei at 30 degrees C or 37 degrees C did not modify the percentage of proteins translocated.
Carvalho
M G
MG
Laboratório do Controle de Expressão Gênica, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.
D000330:Aedes / Q000166:cytology; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006358:Hot Temperature; D066298:In Vitro Techniques
D003470:Culture Media; D006360:Heat-Shock Proteins