Isolation and characterization of cDNA clones for castration-induced mRNAs in the rat ventral prostate.
To identify gene products involved in castration-induced involution of the rat ventral prostate, we constructed a subtraction cDNA library of the ventral prostate from rats castrated for 48 h. The library was screened with subtracted cDNA probes enriched for sequences with a low copy number expressed in intact or castrated rats. As a result of differential screening, 48 cDNA clones representing 10 different induced mRNAs were isolated. The time course of these mRNA inductions after castration was examined. Within the first 24 h after castration, the level of mRNAs for these cDNA clones was significantly increased and it reached its peak by 48-72 h after castration. Although mRNAs for these cDNA clones were expressed in various tissues from intact rats, an increase in mRNA as a response to castration was observed only in the ventral prostate. Partial sequence analyses of the 10 cDNA clones indicate that three cDNA clones represent rat glutathione S-transferase Yb-1, Yb-2 and Yb-3 subunit mRNA sequences, but for others respective homologues could not be found in a search of the GenBank database (release 67).
Hoshikawa
Y
Y
Division of Physiology, Tottori University School of Medicine, Yonago, Japan.
Satoh
Y
Y
Ichii
S
S
D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D015342:DNA Probes; D005982:Glutathione Transferase / Q000235:genetics; D008297:Male; D009919:Orchiectomy; D011467:Prostate / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D015342:DNA Probes; D012333:RNA, Messenger; D004247:DNA; D005982:Glutathione Transferase