[The effect of staphylococcal alpha-toxin on DNA replication in human cells].
The influence of Staphylococcus alpha-toxin has been investigated on the duration of S-phase of lymphocyte mitotic cycle and on DNA replication in human fibroblasts in vitro. The duration of the S-phase of lymphocytes was measured by counting labeled metaphases and by making replication curves. Alpha-toxin in a dose of 3 micrograms/ml enhances the onset of S-phase, which is inhibited at a dose of 33 micrograms/ml of alpha-toxin. The action of alpha-toxin resulted in a decreased rate of replication fork and in a progressive activation of replicon groups. This effect was most prominent at 33 micrograms/ml of alpha-toxin. The data obtained allow to suggest that immunodeficiency of the second order, so characteristic of the staphylococcal sepsis, may be due, in many respects, to suppression of DNA replication.
Khachapuridze
G G
GG
Nergadze
S G
SG
Lapiashvili
N N
NN
Panieva
I N
IN
Barenfel'd
L S
LS
Pleskach
N M
NM
Imedadze
M Sh
MSh
D001345:Autoradiography; D001427:Bacterial Toxins / Q000494:pharmacology*; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006460:Hemolysin Proteins; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D016196:S Phase / Q000187:drug effects; D013211:Staphylococcus aureus
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; C021091:staphylococcal alpha-toxin; D004247:DNA
Vliianie stafilokokkovogo al'fa-toksina na replikatsiiu DNK v kletkakh cheloveka.