title: New rapid method for the study of Pneumocystis carinii interaction with alveolar macrophages.
abstract: Understanding the pathophysiology of Pneumocystis carinii infection has been limited by the availability of methods for precisely measuring the interaction of P. carinii with host cells. Here we describe a new method which allows for the rapid assessment of P. carinii binding to, and internalization by, adherent alveolar macrophages. The method is based on the detection of fluorescein-labelled P. carinii by an automated fluorescence measurement system.
authors.affiliation: MacArthur Center for Molecular Parasitology, Yale University, New Haven, CT.
authors.forename: H
authors.initials: H
authors.lastname: Koziel
authors.forename: D J
authors.initials: DJ
authors.lastname: Williams
authors.forename: M Y
authors.initials: MY
authors.lastname: Armstrong
authors.forename: F F
authors.initials: FF
authors.lastname: Richards
authors.forename: J A
authors.initials: JA
authors.lastname: Fishman
authors.forename: R A
authors.initials: RA
authors.lastname: Ezekowitz
authors.forename: A
authors.initials: A
authors.lastname: Warner
authors.forename: J
authors.initials: J
authors.lastname: Fuglestad
authors.forename: R M
authors.initials: RM
authors.lastname: Rose
mesh_terms: D002478:Cells, Cultured; D016650:Fluorescein-5-isothiocyanate; D005470:Fluorometry; D006801:Humans; D016676:Macrophages, Alveolar / Q000382:microbiology*; D011010:Pneumocystis / Q000276:immunology*
chemical_list: D016650:Fluorescein-5-isothiocyanate