title: Determination of glyphosate and its metabolite, (aminomethyl)phosphonic acid, in serum using capillary electrophoresis.
abstract: Capillary electrophoresis has been used to separate and quantitate glyphosate and its major metabolite, (aminomethyl)phosphonic acid (AMPA), in serum. The two compounds, after derivatization with p-toluenesulphonyl chloride, were clearly separated with 0.1 M boric acid-sodium hydroxide buffer (pH 9.6) containing 10% methanol. The separation was completed within 15 min at an applied potential of 30 kV. Calibration curves for the assay were linear over both the lower (0.5-10 micrograms/ml) and the higher (10-100 micrograms/ml) concentration ranges. The within-run and day-to-day coefficients of variation of peak area were 1.4-4.4 and 4.4-8.5%, respectively, for glyphosate and 1.8-2.9 and 1.8-2.9%, respectively, for AMPA. The within-run and day-to-day precisions of the migration time for both compounds were less than 1.8% and less than 2.5%, respectively. The detection limit of both derivatives was 0.1 microgram/ml in spiked sera, and the recoveries of glyphosate and AMPA were 87.9-88.8 and 78.4-86.9%, respectively. In this study, the reproducibility and the effect of pH changes on the electropherograms were especially examined.
authors.affiliation: Department of Legal Medicine, Kawasaki Medical School, Kurashiki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tomita
authors.forename: T
authors.initials: T
authors.lastname: Okuyama
authors.forename: Y
authors.initials: Y
authors.lastname: Nigo
authors.forename: B
authors.initials: B
authors.lastname: Uno
authors.forename: S
authors.initials: S
authors.lastname: Kawai
mesh_terms: D004586:Electrophoresis / Q000379:methods*; D005998:Glycine / Q000031:analogs & derivatives* / Q000097:blood; D006540:Herbicides / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009943:Organophosphorus Compounds / Q000097:blood*; D015203:Reproducibility of Results; D000097797:Glyphosate
chemical_list: D005998:Glycine; D006540:Herbicides; D009943:Organophosphorus Compounds; D000097797:Glyphosate; C036423:1-aminomethylphosphonic acid