The effect of moderate operative trauma on the blood lymphocyte reactivity--immunomodulatory effect of interleukin-2.
The influence of moderate operative trauma on lymphocyte responsiveness to phytohemagglutin (PHA) and in autologous mixed lymphocyte response (AMLR) was studied. Furthermore, production of interleukin 2 (IL2) and immunomodulatory effect of exogenous IL2 on lymphocyte reactivity after surgery were examined. The responsiveness to PHA declined on day 1 (p less than 0.05) and the level of AMLR was found decreased on days 1 (p less than 0.05), 3 (p less than 0.02) and 5 (p less than 0.03) after surgery. Production of IL2 significantly decreased on day 1, 3 and 5 (p less than 0.05) after operation. Supplementation of PHA and AMLR cultures with exogenous IL2 restored the attenuated postoperative responsiveness to the levels observed preoperatively. This observation may justify IL2 therapy in surgical patients with high risk of uncontrollable postoperative infections.
Grzelak
I
I
Laboratory of Surgical Research and Transplantation, Polish Academy of Sciences, Warsaw.
Olszewski
W L
WL
Rowiński
W
W
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000328:Adult; D002763:Cholecystectomy / Q000009:adverse effects; D006801:Humans; D007108:Immune Tolerance; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis / Q000494:pharmacology*; D008214:Lymphocytes / Q000276:immunology*; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000276:immunology; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D013514:Surgical Procedures, Operative / Q000009:adverse effects*; D013530:Surgical Wound Infection / Q000517:prevention & control
D000276:Adjuvants, Immunologic; D007376:Interleukin-2; D010835:Phytohemagglutinins; D015375:Receptors, Interleukin-2