title: Synthesis and expression in E. coli of the gene for human tumor necrosis factor (h alpha TNF).
abstract: A gene coding for human tumor necrosis factor (h alpha TNF) has been assembled by ligating short oligodeoxyribonucleotides and cloning into plasmid vectors. These oligonucleotides were prepared by the modified phosphoramidite methodology using isopropoxyacetyl (IPA) as a protecting group for exoamino- functions of nucleosides. Gene was expressed in E. coli and the protein product was purified to homogeneity by ion-exchange chromatography.
authors.affiliation: Department of Bioorganic Chemistry, Polish Academy of Sciences, Lódź.
authors.forename: J
authors.initials: J
authors.lastname: Kłysik
authors.forename: A
authors.initials: A
authors.lastname: Konarzewska-Zglińska
authors.forename: G
authors.initials: G
authors.lastname: Gałazka
authors.forename: B
authors.initials: B
authors.lastname: Uznański
authors.forename: A
authors.initials: A
authors.lastname: Okruszek
authors.forename: P
authors.initials: P
authors.lastname: Guga
authors.forename: A
authors.initials: A
authors.lastname: Wilk
authors.forename: M
authors.initials: M
authors.lastname: Koziołkiewicz
authors.forename: H
authors.initials: H
authors.lastname: Tchórzewski
authors.forename: M
authors.initials: M
authors.lastname: Zembala
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D004247:DNA