Conversion of 3H-nitrendipine binding to the low affinity binding state following the treatment of hippocampal slices with morphine.
The effect of morphine on the binding of 3H-nitrendipine was studied in rat hippocampal preparations. Treatment of slices with morphine followed by the preparation of membrane fractions revealed the presence of low affinity binding sites. The effect of morphine was antagonized by naloxone. The effect was not observed when the membrane fraction was incubated with morphine. These results suggest that morphine changes the affinity of calcium for its channels and reduces its influx.
Ohnishi
T
T
Second Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Osaka University, Suita, Japan.
Saito
K
K
Maeda
S
S
Matsumoto
K
K
Sakuda
M
M
Inoki
R
R
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D015636:Magnesium Chloride / Q000494:pharmacology; D008297:Male; D009020:Morphine / Q000494:pharmacology*; D009294:Narcotics / Q000494:pharmacology; D009568:Nitrendipine / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride / Q000494:pharmacology; D014316:Tritium
D009294:Narcotics; D015636:Magnesium Chloride; D014316:Tritium; D012965:Sodium Chloride; D009020:Morphine; D000255:Adenosine Triphosphate; D009568:Nitrendipine; D002118:Calcium