Separation and characterization of three positional isomers of dimaltosyl-cyclomaltoheptaose (dimaltosyl-beta-cyclodextrin).
A mixture of maltosylcyclomaltoheptaoses (maltosyl-beta-cyclodextrins, G2-beta CDs) was prepared from maltose and beta-cyclodextrin (beta CD) through the reverse action of Klebsiella pneumoniae pullulanase. Three positional isomers of dimaltosyl-beta CD in the mixture were separated by high-performance liquid chromatography on a reversed phase column and a graphitized carbon column. Their molecular weights were measured by fast-atom bombardment mass spectrometry, and the structures were established by methylation analysis, hydrolysis with glucoamylase to the known compounds, three positional isomers of diglucosyl-beta CD, and 13C-nuclear magnetic resonance spectroscopy.
Koizumi
K
K
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
Okada
Y
Y
Fujimoto
E
E
Takagi
Y
Y
Ishigami
H
H
Hara
K
K
Hashimoto
H
H
D003505:Cyclodextrins / Q000737:chemistry / Q000302:isolation & purification*; D006026:Glycoside Hydrolases / Q000378:metabolism; D007536:Isomerism; D007711:Klebsiella pneumoniae / Q000378:metabolism; D008320:Maltose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D008970:Molecular Weight; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C068070:6,6-di-O-maltosyl-beta-cyclodextrin; D008320:Maltose; D006026:Glycoside Hydrolases; C100181:pullulanase