title: Monoclonal antibodies against human antithrombin III.
abstract: Three monoclonal antibodies identified as D8, B11 and C5 of different specificities have been produced against human antithrombin III (AT). The apparent dissociation constants (Kd app) of the AT-antibody interaction were determined by ELISA method: Kd app (D8) = 2.4 nmole, Kd app (B11) = 13 nmole, Kd app (C5) = 24 nmole. All three antibodies reacted with isolated AT on immunoblots obtained with "native" PAGE. The D8 antibody also reacted with plasma and serum AT while B11 antibody reacted with serum thrombin-antithrombin (TAT) complexes as well.
authors.affiliation: Institute of Hematology and Transfusion, Praha, Czechoslovakia.
authors.forename: Z
authors.initials: Z
authors.lastname: Hrkal
authors.forename: H
authors.initials: H
authors.lastname: Cajthamlová
authors.forename: J T
authors.initials: JT
authors.lastname: Novák
authors.forename: E
authors.initials: E
authors.lastname: Paluska
authors.forename: P
authors.initials: P
authors.lastname: Stöckbauer
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D000990:Antithrombin III / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D010447:Peptide Hydrolases / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; C046193:antithrombin III-protease complex; D000990:Antithrombin III; D010447:Peptide Hydrolases