title: Determination of 2'-deoxy-5-iodouridine and its metabolite 5-iodouracil by high-performance liquid chromatography with ultraviolet absorbance detection in human serum.
abstract: A new assay is described for 2'-deoxy-5-iodouridine, a drug employed as an antiviral agent by topical application. The parent drug, its systemic metabolite 5-iodouracil and an internal standard (5-iodouridine) were extracted from salted serum by an ethyl acetate partition at pH 6.7, back-extracted in alkalinized water and injected into a reversed-phase column. Potassium phosphate buffer-acetonitrile (95:5, v/v) eluted the analytes at a flow-rate of 1.5 ml/min. Detection was at 290 nm. The method proved to be linear in the 100-2000 ng/ml range.
authors.affiliation: Analytical Research Division of Farma Resa srl, Cantù, Como, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Belotto
authors.forename: V
authors.initials: V
authors.lastname: Reiner
authors.forename: F
authors.initials: F
authors.lastname: Verga
authors.forename: G
authors.initials: G
authors.lastname: Canobbio
authors.forename: F
authors.initials: F
authors.lastname: Lietti
authors.forename: P
authors.initials: P
authors.lastname: Magnani
authors.forename: C
authors.initials: C
authors.lastname: Lucarelli
mesh_terms: D000998:Antiviral Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007065:Idoxuridine / Q000097:blood*; D012016:Reference Values; D013056:Spectrophotometry, Ultraviolet; D014498:Uracil / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D000998:Antiviral Agents; D014498:Uracil; C048747:5-iodouracil; D007065:Idoxuridine