title: Excretion of urinary enzymes in female Sprague-Dawley rats in relation to cellular compartment, creatinine excretion and diuresis.
abstract: One hundred and one young-adult female Sprague-Dawley rats were acclimatized to metabolic cages for 2 days. After that time 24-hour urine was collected at a constant cooling temperature of 0-4 degrees C. After gel filtration the enzyme activities were determined, and the resulting values were used to calculate 24-hour excretions. The following reference ranges (2.5 and 97.5 percentiles) were determined (in mU/24 h): lactate dehydrogenase 43-181; phosphohexoseisomerase 45-1445; glutathione-S-transferase 1-299; alkaline phosphatase 27-1239; leucine arylamidase 72-377; gamma-glutamyltransferase 1334-9188; arylsulphatase A 59-309; beta-galactosidase 76-305; beta-glucuronidase 20-2756; beta-N-acetyl-D-glucosaminidase 66-491; glutamate dehydrogenase 7-711. There was a significant (though not very high) correlation with diuresis for the lysosomal enzymes beta-N-acetyl-D-glucosaminidase, arylsulphatase A and beta-galactosidase, and for glutamate dehydrogenase, lactate dehydrogenase, phosphohexoseisomerase and alkaline phosphatase. The relation to creatinine excretion was markedly close for the lysosomal enzymes beta-N-acetyl-D-glucosaminidase, arylsulphatase A and beta-galactosidase (r = 0.71-0.83), as well as for alkaline phosphatase, leucine arylamidase and gamma-glutamyltransferase. There was a relatively high correlation between the excretion of beta-N-acetyl-D-glucosaminidase, arylsulphatase A and beta-galactosidase among themselves (r = 0.63-0.81) as well as between leucine arylamidase and gamma-glutamyltransferase (r = 0.75).
authors.affiliation: Institute of Industrial Toxicology, Bayer AG, Wuppertal, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Bomhard
authors.forename: D
authors.initials: D
authors.lastname: Maruhn
authors.forename: H
authors.initials: H
authors.lastname: Mager
mesh_terms: D000818:Animals; D002451:Cell Compartmentation / Q000502:physiology*; D002850:Chromatography, Gel; D003401:Creatine / Q000652:urine*; D004231:Diuresis / Q000502:physiology*; D005260:Female; D006867:Hydrolases / Q000652:urine; D007668:Kidney / Q000201:enzymology*; D010088:Oxidoreductases / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D014166:Transferases / Q000652:urine
chemical_list: D010088:Oxidoreductases; D014166:Transferases; D006867:Hydrolases; D003401:Creatine