title: [Use of polymerase chain reaction of the HLA-DQ alpha system in forensic trace analysis].
abstract: The polymerase chain reaction (PCR) can be used for genetic typing of minute amounts of biological stains. This is achieved by in vitro amplification of a well-defined and genetically polymorphic human genomic DNA sequence. Using the HLA-DQ alpha system, a population study was carried out among 212 unrelated individuals of German origin. The usefulness of this system is discussed by presenting examples of its application in forensic casework, i.e. the analysis of mixed (male/female) body fluids as well as segregation studies on embryonic and paraffin-embedded tissue samples.
authors.affiliation: Institut für Rechtsmedizin, Johannes-Gutenberg-Universität zu Mainz.
authors.forename: P M
authors.initials: PM
authors.lastname: Schneider
authors.forename: M
authors.initials: M
authors.lastname: Prager-Eberle
authors.forename: C
authors.initials: C
authors.lastname: Rittner
mesh_terms: D000483:Alleles; D015342:DNA Probes; D005787:Gene Frequency / Q000235:genetics; D005828:Genetics, Population; D005858:Germany; D006683:HLA-DQ Antigens / Q000235:genetics*; D059848:HLA-DQ alpha-Chains; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D015342:DNA Probes; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen
vernacular_title: Zur Anwendung der Polymerase Kettenreaktion (PCR) des HLA-DQ alpha-Systems in der forensischen Spurenkunde.