Augmentation of antiproliferative activity of CPT-11, a new derivative of camptothecin, by tumor necrosis factor against proliferation of gynecologic tumor cell lines.
The co-operative effects of recombinant human tumor necrosis factor (rH-TNF) and CPT-11, a new derivative of camptothecin, against the proliferation of human gynecologic tumor cell lines were examined in vitro. The ishikawa cells were responsive to rH-TNF, the HHUA cells exhibited a minimal degree of responsiveness to rH-TNF, and the HeLa S3 and Caov-3 cells were unresponsive to rH-TNF. The HHUA, Ishikawa and Caov-3 cells were responsive to CPT-11, and the HeLa S3 cells were relatively sensitive to CPT-11 cytotoxicity. In all four cell lines, rH-TNF at clinically achievable concentrations exhibited synergy with CPT-11. The combination therapy of rH-TNF and CPT-11 will be a new approach against gynecologic cancers.
Mori
H
H
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Japan.
Sawairi
M
M
Itoh
N
N
Hanabayashi
T
T
Kondoh
H
H
Tamaya
T
T
D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D002166:Camptothecin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D002455:Cell Division / Q000187:drug effects; D004357:Drug Synergism; D005260:Female; D005833:Genital Neoplasms, Female / Q000188:drug therapy / Q000473:pathology*; D006801:Humans; D000077146:Irinotecan; D011994:Recombinant Proteins / Q000008:administration & dosage; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage*
D000972:Antineoplastic Agents, Phytogenic; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D000077146:Irinotecan; D002166:Camptothecin