Determination of dehydroalanine residues in proteins and peptides: an improved method.
Dehydroalanine residues in peptides and proteins react with 4-pyridoethanethiol in alkaline solution to form 4-pyridoethyl cysteine residues, which after acid hydrolysis, give the corresponding amino acid. An experimental protocol has been developed, and with simple dehydroalanine derivatives, conversions of 96-99% are achieved. Tests with peptides and proteins show that serine and cysteine/cystine residues do not interfere with this analytical procedure. A sample of wool keratin contained 57 mumol dehydroalanine/g.
Bartone
N A
NA
CSIRO Division of Biomolecular Engineering, Parkville, Victoria, Australia.
Bentley
J D
JD
Maclaren
J A
JA
D000409:Alanine / Q000031:analogs & derivatives* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry; D013438:Sulfhydryl Compounds
D010455:Peptides; D011506:Proteins; D011725:Pyridines; D013438:Sulfhydryl Compounds; C074740:4-pyridoethanethiol; C015102:dehydroalanine; D000409:Alanine