Nucleotide sequence of a variant protein A of Staphylococcus aureus suggests molecular heterogeneity among strains.
Protein A is a cell wall linked protein of Staphylococcus aureus that binds mammalian IgG. Although protein A displays high size heterogeneity among strains, cloning and sequencing of its gene from two strains had not shown a large difference in size. Here we report a third protein A gene sequence that shows a size variation relative to these two, due to deletions on both one IgG binding domain and a cell wall binding domain (region X). By analysis of the three sequences we were able to delineate a hypothetic model for region X domain evolution and discussed the origin of genetic variability within and without strains.
Brígido
M de M
Mde M
Ludwig Institute for Cancer Research, São Paulo Branch, Brasil.
Barardi
C R
CR
Bonjardin
C A
CA
Santos
C L
CL
Junqueira
M L
ML
Brentani
R R
RR
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D005075:Biological Evolution; D003001:Cloning, Molecular; D014644:Genetic Variation; D007074:Immunoglobulin G / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013205:Staphylococcal Protein A / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics*
D007074:Immunoglobulin G; D013205:Staphylococcal Protein A