[The purification and concentration of the rabies virus by a diafiltration concentration method].
Purified concentrates of rabies virus were prepared by both microfiltration chromatography and diafiltration methods. The diafiltration concentrate had a high level of protective activity and was not inferior to the microfiltration chromatographic one. The infectious activity was better retained after diafiltration, the chromatographic purity of both preparations was similar. It is concluded that diafiltration presents a good alternative to the currently used methods for purification and concentration of viruses.
Kliushnik
S Iu
SIu
Mchedlishvili
B V
BV
Gribencha
S V
SV
Romanova
L N
LN
Barinskiĭ
I F
IF
D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D051379:Mice; D008808:Mice, Inbred CBA; D011820:Rabies virus / Q000737:chemistry / Q000302:isolation & purification* / Q000472:pathogenicity; D014462:Ultrafiltration / Q000295:instrumentation / Q000379:methods; D014764:Viral Proteins / Q000032:analysis / Q000302:isolation & purification; D014773:Virology / Q000295:instrumentation / Q000379:methods
D014764:Viral Proteins
Ochistka i kontsentrirovanie virusa beshenstva metodom diafil'tratsionnogo kontsentrirovaniia.