Characterization of 2S seed storage protein of Brassica campestris and its antigenic homology with seed proteins of other Cruciferae.
The low molecular weight seed storage protein of Brassica campestris has been isolated and its amino acid composition determined. Antibody raised against this low molecular weight protein has been used to compare the antigenic similarity between the low molecular weight storage proteins of different Cruciferae seeds by immunoprecipitation and Western blotting. These studies revealed the existence of antigenically homologous proteins of identical molecular weights in seeds of other Cruciferae but absent in some other dicots like mung bean and tobacco seeds.
Dasgupta
S
S
Department of Biochemistry, Bose Institute, Calcutta, India.
Mandal
R K
RK
D000596:Amino Acids / Q000032:analysis; D000941:Antigens / Q000032:analysis*; D001937:Brassica / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D008970:Molecular Weight; D009149:Mustard Plant / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry* / Q000276:immunology; D010946:Plants, Medicinal; D012639:Seeds / Q000737:chemistry*
D000596:Amino Acids; D000941:Antigens; D010940:Plant Proteins