Chiral analysis of methadone in plasma by high-performance liquid chromatography.
A method for the chiral high-performance liquid chromatographic analysis of methadone in plasma has been developed. The method employed organic solvent extraction, enantiomeric separation on a Chiral AGP column, and ultraviolet absorption detection at 212 nm. The intra-day variation in the quantification of methadone enantiomers was less than 9% at the 100 ng/ml level, and the values obtained correlated well with those from a gas chromatographic-mass spectrometric method. Results from patients indicate inter- and intra-individual differences in the ratio between l- and d-methadone in plasma during therapy with racemic methadone. In one patient, a higher level of d-methadone in plasma was caused by both faster elimination and lower bioavailability of l-methadone.
Beck
O
O
Department of Clinical Pharmacology, Karolinska Hospital, Stockholm, Sweden.
Boreus
L O
LO
Lafolie
P
P
Jacobsson
G
G
D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008691:Methadone / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D008691:Methadone