Nucleotide sequences of cDNA clones, pSv-1 and pSv-2, hybridizing to androgen-stimulated mRNAs in rat seminal vesicles.
In order to further characterize the cDNA clones, pSv-1 and pSv-2, which had been newly isolated from a cDNA library of intact rat seminal vesicles as clones hybridizing to androgen-stimulated mRNAs of approximately 1,500 and 3,500 nucleotides in length, respectively, the whole nucleotide sequences were determined. The pSv-1 and pSv-2 were 1135 and 1819 nucleotides in length, respectively, and seemed not to contain entire sequences corresponding to the mRNAs. When the 0.7 kb HindIII fragments from pSv-1 and the 1 kb fragments from pSv-2 were used to probe rat genomic DNA that had been digested with four restriction enzymes, the Southern blots suggested the existence of multiple genes related to pSv-1 and a single gene related to pSv-2. These results suggest that pSv-1 and pSv-2 provide useful probes not only for further characterization of products encoded by the mRNAs, but also for the study on the physiological roles of androgen-dependent gene expression in rat seminal vesicles.
Izawa
M
M
Division of Physiology, Faculty of Medicine, Tottori University, Yonago, Japan.
D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000235:genetics*; D015342:DNA Probes; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D012669:Seminal Vesicles / Q000737:chemistry*
D000728:Androgens; D015342:DNA Probes; D012333:RNA, Messenger; D004247:DNA