The action of the couple ascorbic acid--Cu(II) on NAD(+)-coenzymatic function.
The coenzymatic capacity of NAD+ decreases by exposing it to the action of ascorbic acid--Cu(II) system, a generator of free radicals. The participation of the radicals in degrading NAD+ depends on the nature of the buffer, incubation medium pH, temperature, incubation time. Thiourea, IK, GSH, cysteine, mannitol, albumine reduce to different extents the noxious action of the radicals. The decrease of the coenzymatic function of NAD+ is caused by the break of esther and anhydride links, evidenced by releasing th cian-sensitive compounds--nicotinamide riboside and nicotinamide ribonucleotide. Also the presence of ortophosphate was demonstrated.
Filip
M
M
Department of Biochemistry, University of Medicine and Pharmacy Iaşi.
Păduraru
I
I
D001205:Ascorbic Acid / Q000494:pharmacology*; D002021:Buffers; D003300:Copper / Q000494:pharmacology*; D004795:Enzyme Stability / Q000187:drug effects; D005609:Free Radicals; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009243:NAD / Q000187:drug effects*; D013696:Temperature; D013997:Time Factors
D002021:Buffers; D005609:Free Radicals; D009243:NAD; D003300:Copper; D001205:Ascorbic Acid