Two-dimensional polyacrylamide gel electrophoresis of proteins synthesized during early germination of Bacillus subtilis 168 in the presence of actinomycin D.
Two-dimensional gel electrophoresis was used to demonstrate the synthesis of approximately 65 [35S]-methionine-labelled soluble proteins between 0 and 10 min after the start of germination, of approximately 210 proteins at 10-20 min, and of approximately 260 proteins during vegetative growth of Bacillus subtilis. When actinomycin D and [35S]-methionine were administered at the onset of germination and the proteins synthesized during the subsequent 15 min were analyzed, two proteins were detected, and were designated protein I and protein II. Immunoblot analysis with an antiserum raised against RNA polymerase from Escherichia coli demonstrated that protein II corresponded to the sigma A factor of Bacillus subtilis. Thus, the sigma A factor is synthesized during early germination of Bacillus subtilis in the presence of actinomycin D.
Hirano
Y
Y
Department of Molecular Biology, Kanazawa University Cancer Research Institute, Japan.
Matsuda
M
M
Kameyama
T
T
D001412:Bacillus subtilis / Q000187:drug effects / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002455:Cell Division / Q000502:physiology; D012321:DNA-Directed RNA Polymerases / Q000276:immunology; D003609:Dactinomycin / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000378:metabolism; D005779:Immunodiffusion; D012808:Sigma Factor / Q000096:biosynthesis; D013171:Spores, Bacterial / Q000187:drug effects / Q000378:metabolism*
D001426:Bacterial Proteins; D012808:Sigma Factor; D003609:Dactinomycin; D012321:DNA-Directed RNA Polymerases