Human pheochromocytoma cells studied in culture contain large amounts of DSIP-like material.
Delta sleep-inducing peptide (DSIP)-like immunoreactive (LI) material has been detected in nine different human pheochromocytoma tumors by immunocytochemistry. In primary tumors subjected to indirect immunofluorescence a variable number of tumor cells (25-75%) showed positive cytoplasmic labeling after incubation with DSIP antiserum. Tumor cells grown in culture were strongly labeled by the DSIP antiserum with DSIP-LI concentrated to cell bodies. Electron microscopic immunocytochemistry (immunogold labeling) of pheochromocytoma cells demonstrated DSIP-LI over the dense core of secretory granules. The presence of DSIP-LI in several HPLC fractions from conditioned culture media indicates secretion of DSIP-LI from cultured pheochromocytoma cells. The observations suggest that DSIP-LI is synthesized and stored in secretory granules before release. The different HPLC profiles from each of the tumors may reflect differences in processing or turnover of DSIP-LI in pheochromocytoma cells.
Nilsson
O
O
Department of Histology, University of Göteborg, Sweden.
Wängberg
B
B
Wigander
A
A
Lundmark
K
K
Dahlström
A
A
Ahlman
H
H
Bjartell
A
A
Ekman
R
R
D000310:Adrenal Gland Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D002851:Chromatography, High Pressure Liquid; D046508:Culture Techniques / Q000379:methods; D003701:Delta Sleep-Inducing Peptide / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D016253:Microscopy, Immunoelectron; D010673:Pheochromocytoma / Q000473:pathology* / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
D003701:Delta Sleep-Inducing Peptide