Chemical, molecular biology, and genetic techniques for correlating DNA base damage induced by ionizing radiation with biological end points.
The types of DNA base damage induced by ionizing radiation, and also relevant model system investigations on replication and mutagenesis, are reviewed in this paper. Recent advances in DNA synthesis technology and site-directed mutagenesis suggest that these methods can be profitably utilized to correlate specific types of DNA base damage with selected biological end points. A deeper insight can be obtained into the molecular origins of mutations, and the effects of base sequence surrounding the lesions on the nature and types of mutations.
Geacintov
N E
NE
Chemistry Department, New York University, New York.
Swenberg
C E
CE
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D005821:Genetic Techniques; D006801:Humans; D008967:Molecular Biology / Q000379:methods; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009838:Oligodeoxyribonucleotides / Q000528:radiation effects; D011839:Radiation, Ionizing
D009838:Oligodeoxyribonucleotides; D004247:DNA