[A study on autoimmune response against spermatozoa following intravas deferens injection of high molecular hydrogel HFMC in rabbits].
To investigate whether intravas injection of HFMC can result in antisperm antibodies, we divided 30 fertile male rabbits into the DMSO, 10% HFMC and 20% HFMC groups. Two to 8 months after intravas injection of the agents, we collected the sera per month and used ELISA to detect the serum antisperm antibodies, The results showed that there are lower antisperm antibody levels in the sera of DMSO, 10% HEMC and 20% HFMC groups in the first 2 months (P less than 0.05), but no significant difference between the antibody levels of DMSO, 10% and 20% HFMC groups and that of the control group from the 3rd month to the 8th month (P greater than 0.05). No immune-related pathological changes were found in the testis and epididymis. Intravas injection of HFMC in the rabbits appears to be a safe method of contraception that causes no deleterious effects.
He
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Y
Sichuan Family Planning Research Institute.
Luo
Z
Z
Xu
C
C
D000818:Animals; D001323:Autoantibodies / Q000097:blood*; D001814:Blood-Testis Barrier / Q000187:drug effects; D003272:Contraceptive Agents, Male / Q000633:toxicity*; D007267:Injections; D008297:Male; D008689:Methacrylates / Q000633:toxicity*; D011109:Polymethacrylic Acids / Q000633:toxicity*; D011817:Rabbits; D013094:Spermatozoa / Q000276:immunology*; D014649:Vas Deferens
D001323:Autoantibodies; D003272:Contraceptive Agents, Male; D008689:Methacrylates; D011109:Polymethacrylic Acids; C063229:hydroxyethyl methacrylate-methacrylic acid-ethyl methacrylate copolymer