[Genetic system of selecting point mutations of bacteriophage T7 RNA polymerase].
Random mutagenesis of the bacteriophage T7 gene 1 was used to delineate the functionally important amino acids residues of its product--T7 RNA polymerase. A two-plasmid system has been constructed for the phenotypic selection of the mutants. Nine mutants were isolated; the RNA polymerase with Tyr-639----Asp substitution has been shown to be completely inactive. Methods were developed for the rapid purification of plasmid-encoded mutant proteins, facilitating their future biochemical analysis.
Rechinskiĭ
V O
VO
Dragan
S M
SM
Kostiuk
D A
DA
Liakhov
D L
DL
Kochetkov
S N
SN
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid; D013604:T-Phages / Q000201:enzymology*
D012321:DNA-Directed RNA Polymerases
Geneticheskaia sistema otbora tochechnykh mutantov RNK-polimerazy bakteriofaga T7.