Enantiospecific analysis of ketoprofen in plasma by high-performance liquid chromatography.
A high-performance liquid chromatographic (HPLC) assay for the determination of the R- and S-enantiomers of ketoprofen is described. Facile ketoprofen extraction from plasma and derivatization to the diastereomeric S-1-phenylethylamides was followed by normal-phase HPLC. The ketoprofen diastereomeric amides eluted within 8 min. The limit of quantification of the assay was 0.15 mg/l of each enantiomer (signal-to-noise ratio = 5).
Hayball
P J
PJ
Pharmacy Department, Repatriation General Hospital, Daw Park, SA, Australia.
Nation
R L
RL
Bochner
F
F
Le Leu
R K
RK
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007660:Ketoprofen / Q000097:blood*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D007660:Ketoprofen