Purification of the multicatalytic proteinase from the nucleus and cytoplasm of chicken red blood cells.
We have purified the multicatalytic proteinase from the nucleus and cytoplasm of chicken red blood cells. Both enzymes have an Mr of 700 kDa and can be separated into 8-10 bands (22-32 kDa) on 1D-SDS-PAGE.
Strack
P R
PR
Department of Animal Sciences, Rutgers University, New Brunswick, NJ 08903.
Wajnberg
E F
EF
Waxman
L
L
Fagan
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D002645:Chickens; D003546:Cysteine Endopeptidases / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D003593:Cytoplasm / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D009842:Oligopeptides / Q000737:chemistry; D046988:Proteasome Endopeptidase Complex; D013379:Substrate Specificity
D009097:Multienzyme Complexes; D009842:Oligopeptides; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex