title: KCl cotransport in HbAA and HbSS red cells: activation by intracellular acidity and disappearance during maturation.
abstract: Low intracellular pH was shown to be a potent activator of the KCl cotransport system in HbSS red cells, and in reticulocyte-rich fractions of HbAA red cells. Rheological experiments indicated that cell dehydration via the KCl cotransporter in response to low pH decreased the filterability of HbSS red cells. In vitro maturation experiments showed that the KCl cotransport system was rendered cryptic rapidly, in contrast to choline transport, and serine transport via system ASC, which disappeared much more slowly.
authors.affiliation: Department of Physiology, University of Oxford.
authors.forename: J C
authors.initials: JC
authors.lastname: Ellory
authors.forename: A C
authors.initials: AC
authors.lastname: Hall
authors.forename: S A
authors.initials: SA
authors.lastname: Ody
authors.forename: C E
authors.initials: CE
authors.lastname: Poli de Figueiredos
authors.forename: S
authors.initials: S
authors.lastname: Chalder
authors.forename: J
authors.initials: J
authors.lastname: Stuart
mesh_terms: D000755:Anemia, Sickle Cell / Q000097:blood*; D001692:Biological Transport / Q000187:drug effects; D002034:Bumetanide / Q000494:pharmacology; D002352:Carrier Proteins / Q000097:blood*; D002794:Choline / Q000097:blood; D003681:Dehydration; D004905:Erythrocyte Aging; D004907:Erythrocyte Deformability; D004911:Erythrocyte Volume; D006432:Hemochromatosis / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid; D009994:Osmolar Concentration; D010042:Ouabain / Q000494:pharmacology; D011189:Potassium Chloride / Q000097:blood*; D012156:Reticulocytes / Q000378:metabolism; D012694:Serine / Q000097:blood; D027981:Symporters; D000096922:K Cl- Cotransporters
chemical_list: D002034:Bumetanide; D002352:Carrier Proteins; D002794:Choline; D010042:Ouabain; D011189:Potassium Chloride; D012694:Serine; D027981:Symporters; D000096922:K Cl- Cotransporters