Rapid method for the determination of ketanserin in rat serum by high-performance liquid chromatography with fluorimetric detection.
A simplified high-performance liquid chromatographic (HPLC) assay for the determination of ketanserin in rat serum is described. The chromatographic method allowed complete resolution of ketanserin from two of its metabolites. A protein precipitation extraction procedure was employed which allowed rapid sample preparation for injection into the HPLC system. Both intra- and inter-assay coefficients of variation at serum ketanserin concentration of 200 and 800 ng/ml were less than 6% and the accuracy was excellent. The assay has been applied for determining the elimination kinetics of ketanserin in the rat.
Wong
Y W
YW
Department of Pharmacology, University of Texas Health Science Center, San Antonio 78284.
Skinner
M H
MH
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005470:Fluorometry / Q000379:methods*; D007650:Ketanserin / Q000097:blood*; D051381:Rats
D007650:Ketanserin