Metabolic analysis of galactose toxicity in Escherichia coli with 2-deoxygalactose as the probe.
Biochemical basis of galactose toxicity has been studied in gal T mutants (CGSC 4974) using 2-deoxygalactose, a non-metabolizable analogue of galactose, as the probe. It is found that biochemical features of toxicity in wild type cells either with 2-deoxygalactose or with 2-deoxyglucose are very similar to the picture obtained with gal T mutants and the observed bacteriostasis is probably due to futile phosphorylation and not due to any specific inhibitory effect of phosphorylated galactose.
Raut
N
N
Massachusetts Institute of Technology, Cambridge.
Bhaduri
A
A
D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D005643:Fucose / Q000633:toxicity; D005690:Galactose / Q000378:metabolism / Q000633:toxicity*; D015335:Molecular Probes; D009154:Mutation; D010766:Phosphorylation
D015335:Molecular Probes; D005643:Fucose; D005690:Galactose