Determination of methanol in whole blood by capillary gas chromatography with direct on-column injection.
A gas chromatographic procedure was developed for the determination of methanol in small-volume whole blood samples. Samples (100-200 microliters) were prepared by protein precipitation, with direct injection of the supernatant on a wide-bore capillary column. The recovery of methanol and acetonitrile (the internal standard) was approximately 90% and did not vary with sample volume. The assay was linear from 2 micrograms/ml (the limit of detection) through 1000 micrograms/ml and was highly reproducible (intra-day coefficient of variation less than 2.5%). Assay performance was assessed following exposure of rats to methanol. The results indicate that the present procedure is suitable for studies of methanol disposition in small rodent species.
Pollack
G M
GM
Division of Pharmaceutics, School of Pharmacy, University of North Carolina, Chapel Hill 27599-7360.
Kawagoe
J L
JL
D000818:Animals; D002849:Chromatography, Gas; D000432:Methanol / Q000097:blood*; D051381:Rats; D015203:Reproducibility of Results
D000432:Methanol