Mannose 6-phosphate potentiates insulin-like growth factor II effects in cultured human neuroblastoma cells.
Insulin-like growth factor II (IGF-II) and mannose 6-phosphate (man-6-P) bind to distinct sites on the same receptor. In the present study, we examined the effects of man-6-P on the growth promoting effects of IGF-II on SH-SY5Y cultured human neuroblastoma cells. Man-6-P alone increased cell number and neurite outgrowth by approximately 50%; as previously observed, IGF-II increased cell number and neurite outgrowth by approximately 110 and 30%, respectively. However, when cells were grown in the presence of both ligands, cell number increased by 330% and neurite outgrowth by 130%. These results suggest that man-6-P can potentiate the known growth promoting effects of IGF-II on human neuroblastoma cells. Furthermore, they indicate that the IGF-II/man-6-P receptor may serve as a means of integrating distinct growth promoting signals in neuronal cells.
Feldman
E L
EL
University of Michigan, Neuroscience Laboratory, Ann Arbor 48104-1687.
Randolph
A E
AE
D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects; D004357:Drug Synergism; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology*; D007700:Kinetics; D008360:Mannosephosphates / Q000494:pharmacology*; D009447:Neuroblastoma; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology
D008360:Mannosephosphates; D011994:Recombinant Proteins; C027693:mannose-6-phosphate; D007335:Insulin-Like Growth Factor II