title: Spectrophotometric determination of glutathione and of its oxidation product in pharmaceutical dosage forms.
abstract: A simple and sensitive spectrophotometric method suitable for the stability control of pharmaceutical dosage forms containing glutathione (gamma-glutamyl-cysteinyl-glycine), GSH, is described. Besides GSH, the method quantitatively determines its oxidation product, GSSG. The colour reactions of GSH and GSSG with ammonium tetrachloropalladate have been investigated and the optimum reaction conditions, spectral characteristics and composition of the yellow water-soluble complexes have been established. The assay results of pharmaceutical formulations showed good accuracy and precision over the concentration range of 5 x 10(-5)-6 x 10(-4) M GSH.
authors.affiliation: Department of Pharmaceutical Sciences, University of Bologna, Italy.
authors.forename: M A
authors.initials: MA
authors.lastname: Raggi
authors.forename: L
authors.initials: L
authors.lastname: Nobile
authors.forename: A G
authors.initials: AG
authors.lastname: Giovannini
mesh_terms: D000595:Amino Acid Sequence; D002138:Calibration; D004304:Dosage Forms; D005978:Glutathione / Q000031:analogs & derivatives* / Q000032:analysis*; D019803:Glutathione Disulfide; D008969:Molecular Sequence Data; D012680:Sensitivity and Specificity; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004304:Dosage Forms; D005978:Glutathione; D019803:Glutathione Disulfide