[Evaluation of the activity of cytokine genes--a new diagnostic test in immunology].
The increased level of IL-1 mRNA was discovered in neutrophils of patients suffering from pollinosis for a long time and responding poorly to specific immunotherapy. Moreover, neutrophils from patients with exacerbation of pollinosis responded to stimuli less than cells from healthy donors. In remission, the activated neutrophils from pollinosis patients and healthy donors had the same levels of IL-1b mRNA. Tumor necrosis factor alpha expression was unchanged in neutrophils of pollinosis patients. Changes in the status of cells from patients were detected with a simple procedure of dot hybridization. Peripheral blood cells were used as available material. This allows one to hope that the approach can be used for analyzing the patients' immune status in choosing the treatment course as well as for controlling the treatment effect.
Kupriianova
O A
OA
Mordvinov
V A
VA
Ivanova
I P
IP
Krugleeva
O L
OL
Tananko
E M
EM
Braga
E A
EA
Nosikov
V V
VV
Kozlov
V A
VA
Lozovoĭ
V P
VP
D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis / Q000097:blood / Q000235:genetics*; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D009693:Nucleic Acid Hybridization / Q000502:physiology*; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D006255:Rhinitis, Allergic, Seasonal / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology
D007375:Interleukin-1; D015347:RNA Probes; D012333:RNA, Messenger
Otsenka aktivnosti genov tsitokinov--novyĭ diagnosticheskiĭ test v immunologii.