Double-label analyses demonstrating the non-coexistence of enkephalin and glycine in amacrine cells of the larval tiger salamander retina.
Enkephalin immunocytochemistry was combined with either glycine immunocytochemistry or autoradiography of high-affinity glycine uptake to examine for colocalization of enkephalin and glycine in amacrine cells of the larval tiger salamander retina. A total of 995 enkephalin-immunoreactive amacrine cells were visualized in double-label preparations. None of the enkephalin-labelled cells was observed to co-label for markers of glycinergic activity.
Watt
C B
CB
Alice R. McPherson Laboratory of Retina Research, Center for Biotechnology, Baylor College of Medicine, The Woodlands, TX 77381.
Florack
V J
VJ
D000818:Animals; D001345:Autoradiography; D004745:Enkephalins / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D005998:Glycine / Q000032:analysis* / Q000378:metabolism; D007150:Immunohistochemistry; D007814:Larva; D012160:Retina / Q000166:cytology*; D014316:Tritium; D014562:Caudata
D004745:Enkephalins; D014316:Tritium; D005998:Glycine