title: Association of Lps gene with natural resistance of mouse macrophages against Legionella pneumophila.
abstract: Peritoneal macrophages obtained from lipopolysaccharide (LPS)-low responder C3H/HeJ mice (J) permitted the intracellular growth of the bacterium in macrophages of (J x N) F1 progeny was between the parent strains, showing that the traits were co-dominantly expressed. Correlation between intracellular bacterial growth in macrophages and LPS response of spleen cells was examined. Negative correlation was found between the two factors in F2, (J x F1) backcross and (N x F1) backcross progeny. This result implies that Lps gene controls the innate resistance of murine macrophages against the bacteria. Although macrophages of A/J strain also permit intracellular growth of L. pneumophila, gene complementation analysis of A/J and C3H/HeJ mice made clear that the gene control in C3H/HeJ differs from that of A/J strain. Macrophages of C57BL/10ScN, which is LPS-low responder line obtained from C57BL/10, were also defective in controlling the bacterial growth when compared to C57BL/10 mice. We suggest that the Lps gene also controls the natural resistance of murine macrophages against L. pneumophila.
authors.affiliation: Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
authors.forename: Y
authors.initials: Y
authors.lastname: Goto
authors.forename: H
authors.initials: H
authors.lastname: Miyamoto
authors.forename: H
authors.initials: H
authors.lastname: Fujio
authors.forename: Y
authors.initials: Y
authors.lastname: Mizuguchi
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D005260:Female; D005796:Genes; D005816:Genetic Complementation Test; D007111:Immunity, Cellular; D007113:Immunity, Innate; D016952:Legionella pneumophila; D007877:Legionnaires' Disease / Q000235:genetics* / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology
chemical_list: D008070:Lipopolysaccharides