High-performance liquid chromatographic analysis of 3'-azido-3'-deoxythymidine monophosphate diglyceride, an anti-HIV glycerophospholipid.
A reversed-phase chromatographic method is described for the analysis of an experimental anti-AIDS drug 3'-azido-3'-deoxythymidine monophosphate diglyceride (AZT-MP-DG) [J.M. Steim et al., Biochem. Biophys. Res. Commun. 171, 458-464 (1990)] [1], a phosphatidic acid derivative of AZT. Analytical conditions were based upon conventional separations of glycerophospholipid species. Where AZT-MP-DG was monitored by UV absorption, there were two wavelength maxima. The response was linear in the concentration range used in this study. The peak was characterized by absorbance ratios with a rapid scanning UV detector.
Amari
J V
JV
Department of Chemistry, University of Rhode Island, Kingston 02881.
Brown
P R
PR
Pivarnik
P E
PE
Sehgal
R K
RK
Turcotte
J G
JG
D000163:Acquired Immunodeficiency Syndrome / Q000188:drug therapy*; D000998:Antiviral Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D054306:Dideoxynucleotides; D006801:Humans; D015394:Molecular Structure; D010712:Phosphatidic Acids / Q000032:analysis*; D015203:Reproducibility of Results; D015215:Zidovudine / Q000031:analogs & derivatives* / Q000032:analysis
D000998:Antiviral Agents; D054306:Dideoxynucleotides; D010712:Phosphatidic Acids; C075088:3'-azido-3'-deoxythymidine monophosphate diglyceride; D015215:Zidovudine