A study on the origins of urinary serotonin and tryptamine.
A sensitive gas chromatography/negative ion chemical ionization mass spectrometric (GC/NICIMS) method was devised and by using deuterated L-tryptophan-3,3-d2(Trp-d2) as a tracer, the detailed in vivo metabolism of serotonin (5-HT) and tryptamine (TA) was investigated. A human was administered orally with 10 mg/kg Trp-d2 and rats were injected intra-peritoneally with 50 mg/kg Trp-d2. The ratios of the level of 5-HT-d2 derived from Trp-d2 to that of endogenous 5-HT-d0 and that of TA-d2 to that of TA-d0 were measured in urine and some organs of rats at times up to 4 hr after administration. It is concluded that urinary excretion of 5-HT might reflect its turnover in the central nervous system and that of TA might reflect its peripheral turnover.
Matsuda
F
F
National Center for Nervous, Mental and Muscular Disorders, Tokai University School of Medicine, Kanagawa, Japan.
Hayashi
T
T
Naruse
H
H
D000284:Administration, Oral; D000328:Adult; D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007263:Infusions, Parenteral; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000097:blood / Q000652:urine*; D014018:Tissue Distribution; D014363:Tryptamines / Q000097:blood / Q000652:urine*; D014364:Tryptophan / Q000097:blood / Q000378:metabolism
D014363:Tryptamines; D012701:Serotonin; C030820:tryptamine; D014364:Tryptophan