Liquid chromatographic analysis of propranolol enantiomers in human blood using precolumn derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate.
A method for the determination of the R-(+) and S-(-) enantiomers of propranolol in blood was developed. After extraction with heptane-isopentanol and derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate, excess reagent was removed using solid-phase extraction. The enantiomers were separated on an achiral, reversed-phase, radially compressed column, and detected by fluorescence with excitation and emission wavelengths of 260 and 340 nm, respectively. The limit of quantification was 0.5 ng/ml. This method was used for pharmacokinetic analysis of propranolol enantiomers after administration of immediate-release (80 mg) or sustained-release (160 mg) racemic propranolol.
Roux
A
A
Laboratoire de Toxicologie et de Pharmacocinétique, Hôpital Ambroise Paré, Boulogne Billancourt, France.
Blanchot
G
G
Baglin
A
A
Flouvat
B
B
D002851:Chromatography, High Pressure Liquid; D005449:Fluorenes / Q000737:chemistry*; D006801:Humans; D011433:Propranolol / Q000097:blood* / Q000737:chemistry; D012016:Reference Values; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
D005449:Fluorenes; C052812:1-(9-fluorenyl)ethyl chloroformate; D011433:Propranolol