Lipids in yeast cell wall glucans.
Lipids isolated from glucans of Saccharomyces cerevisiae were analysed by thin-layer chromatography, infrared spectroscopy and gas-liquid chromatography. Localization of glucan lipid in the polysaccharide net was observed by light and electron microscopy. Despite using Manner's method for glucan preparation, the residue of acidic lipid occupied a central position in the treated cells. Analysis showed the presence of free fatty acids in which a relatively high content of vaccenic acid was detected.
Sajbidor
J
J
Department of Biochemical Technology, Slovak Technical University, Faculty of Chemistry, Bratislava, Czechoslovakia.
Kopecká
M
M
Sandula
J
J
Kacuráková
M
M
D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis; D005936:Glucans / Q000737:chemistry*; D008055:Lipids / Q000032:analysis* / Q000302:isolation & purification; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D011134:Polysaccharides / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000648:ultrastructure; D013055:Spectrophotometry, Infrared
D005227:Fatty Acids; D005936:Glucans; D008055:Lipids; D011134:Polysaccharides