title: Solid-phase extraction of midazolam and two of its metabolites from plasma for high-performance liquid chromatographic analysis.
abstract: A rapid, sensitive and selective assay of midazolam and two of its metabolites in plasma, based on high-performance liquid chromatography, has been developed. The compounds are subjected to solid-phase extraction, using C18 cartridges (Bond-Elut). Recoveries are in excess of 90% for midazolam and its metabolites. The limit of quantitation of the assay is 50 ng/ml of plasma for each compound.
authors.affiliation: Laboratoire de Pharmacologie et Pharmacie Clinique et Biotechniques, U.F.R. Pharmacie, Clermont-Ferrand, France.
authors.forename: V
authors.initials: V
authors.lastname: Sautou
authors.forename: J
authors.initials: J
authors.lastname: Chopineau
authors.forename: M P
authors.initials: MP
authors.lastname: Terrisse
authors.forename: P
authors.initials: P
authors.lastname: Bastide
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008874:Midazolam / Q000031:analogs & derivatives* / Q000097:blood*
chemical_list: C040081:1-hydroxymethylmidazolam; D008874:Midazolam