Competition of homologous substrates, putrescine and cadaverine, in the reaction catalyzed by pea diamine oxidase.
The determination of diamine oxidase activity with the ninhydrin reagent was used for monitoring of simultaneous oxidation of two homologous substrates, putrescine and cadaverine, which give different colour products (519 and 417 nm). We measured the reaction rates of oxidation of both substrates in different proportion and compared them with the total reaction rate determined by the guaiacol method. The substrates show competition with inhibition constants of putrescine against cadaverine of 0.14 mmol.l-1 and cadaverine against putrescine of 6.4 mumol.l-1.
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Department of Analytical and Organic Chemistry, Faculty of Science, Palacký University, Olomouc, Czechoslovakia.
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D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism*; D001667:Binding, Competitive; D002103:Cadaverine / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007887:Fabaceae; D007700:Kinetics; D010084:Oxidation-Reduction; D010946:Plants, Medicinal; D011700:Putrescine / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013057:Spectrum Analysis; D013379:Substrate Specificity
D006631:Amine Oxidase (Copper-Containing); D002103:Cadaverine; D011700:Putrescine