[The effect of different methods of isolating thrombocytes on the surface structure and biochemical parameters of the serotonin system of these cells].
The method of platelets' isolation influences their morphofunctional state. The study of the surface structure of platelets with the method of scanning electron microscopy shows, that the nonactivated form of platelets is characterized for the cells, isolated by gel filtration, but platelets which are isolated by centrifugation are activated. Platelets' activation under centrifugation is shown to connect with the changes of biochemical parameters of platelet serotonin system: the increase of the velocity of the 3H-serotonin reuptake and of the 3H-imipramine specific binding.
Kiktenko
A I
AI
Zlobina
G P
GP
Shchurin
M R
MR
Kleshchinov
V N
VN
Brusov
O S
OS
D000328:Adult; D001792:Blood Platelets / Q000378:metabolism / Q000648:ultrastructure*; D002469:Cell Separation / Q000379:methods; D002498:Centrifugation / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D005260:Female; D006801:Humans; D007099:Imipramine / Q000493:pharmacokinetics; D008855:Microscopy, Electron, Scanning; D015539:Platelet Activation; D012016:Reference Values; D012701:Serotonin / Q000097:blood*; D013499:Surface Properties
D012701:Serotonin; D007099:Imipramine
Vliianie raznykh sposobov vydeleniia trombotsitov na poverkhnostnuiu strukturu i biokhimicheskie parametry serotoninovoĭ sistemy étikh kletok.