Isolation of amniotic fluid proteins of non-maternal serum origin by negative immuno-affinity chromatography.
Although several proteins of non-maternal serum origin are known to exist in amniotic fluid, the predominance of serum proteins has hampered the isolation of all but a very few. This report describes the removal of large amounts contaminating serum proteins from amniotic fluid with anti-human serum antibody coupled to a solid matrix, leaving behind small amounts of proteins of interest, about 3% of the total amniotic fluid proteins. An antibody raised against the non-adsorbed proteins and used in immunoelectrophoretic methods permitted detection of more, probably unknown, proteins in amniotic fluid of potential biological and diagnostic interest.
Sørensen
S
S
Department of Clinical Chemistry, Hvidovre Hospital, University of Copenhagen, Denmark.
D000649:Amniocentesis; D000653:Amniotic Fluid / Q000737:chemistry*; D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional / Q000379:methods; D007136:Immunoglobulins; D007202:Indicators and Reagents; D008970:Molecular Weight; D011247:Pregnancy; D011506:Proteins / Q000302:isolation & purification*; D011817:Rabbits / Q000276:immunology
D007136:Immunoglobulins; D007202:Indicators and Reagents; D011506:Proteins