title: Tg (9 HSA-MYC), a homozygous lethal insertion in the mouse.
abstract: Transgenic mice generated with different DNA sequences were surveyed for possible homozygous mutant phenotypes. We found an embryonic lethal mutation in the transgenic mouse strain (MT-MYC12.4) containing the human c-myc gene. Embryos homozygous for the transgene die shortly after implantation. The strain MT-MYC12.4 carries approximately 50 tandem copies of the recombinant plasmid sequence. The 3' flanking sequence has been cloned and analyzed. It contains a unique sequence that has been conserved during evolution and maps to Chromosome (Chr) 9. This mutant has been designated Tg 9 (HSA-MYC).
authors.affiliation: Unité de Génétique des Mammifères, Institut Pasteur, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Tutois
authors.forename: J
authors.initials: J
authors.lastname: Salaun
authors.forename: M G
authors.initials: MG
authors.lastname: Mattei
authors.forename: J L
authors.initials: JL
authors.lastname: Guénet
mesh_terms: D000818:Animals; D001483:Base Sequence; D002503:Centromere; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004622:Embryo, Mammalian / Q000033:anatomy & histology; D005314:Embryonic and Fetal Development / Q000235:genetics*; D005804:Genes, Lethal / Q000235:genetics*; D006720:Homozygote; D051379:Mice / Q000196:embryology / Q000235:genetics*; D008822:Mice, Transgenic / Q000196:embryology / Q000235:genetics; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional