The effects of Na+ replacement on intracellular pH and [Ca2+] in rabbit salivary gland acinar cells.
1. The role of Na(+)-dependent mechanisms in regulating the intracellular pH (pHi) and free calcium concentration ([Ca2+]i) in acinar cells of the rabbit mandibular salivary gland was examined. The fluorescent dyes BCECF and Fura-2 were used to measure pHi and [Ca2+]i respectively in suspensions of isolated acini. 2. Replacement of all the extracellular Na+ with N-methyl-D-glucamine (NMDG) decreased resting pHi from a control value of 7.1-7.2 to 6.8-6.9. Re-addition of Na+ or Li+ caused a recovery of pHi towards control values. This recovery was blocked by 10-50 microM-ethylisopropylamiloride (EIPA), suggesting that it was mediated by Na(+)-H+ exchange. The rate of recovery of pHi when Na+ was re-introduced increased with Na+ concentration with an apparent Km for Na+ of around 30 mM. 3. Replacement of all of the extracellular Na+ with Li+ caused only a small decrease in resting pHi. 4. Stimulation of acini with 1 microM-acetylcholine (ACh) evoked an intracellular acidosis both under control conditions and when acini were bathed in Na(+)-free media. Following the acidosis pHi recovered in acini bathed in either control medium or Na(+)-free (Li+) medium, but not in acini bathed in Na(+)-free (NMDG) medium or in control medium containing EIPA. 5. Stimulation of acini bathed in Na(+)-free, HCO(3-)-free medium with ACh did not cause any change in pHi. 6. Re-addition of Na+ to acini bathed in Na(+)-free, HCO(3-)-free medium evoked the same rate of alkalinization whether or not the acini had been stimulated with ACh, suggesting that receptor stimulation per se did not lead to an activation of acid extrusion. 7. Resting [Ca2+]i was elevated in acini bathed in Na(+)-free (NMDG) medium, but not in acini bathed in Na(+)-free (Li+) medium. 8. ACh evoked a maintained rise in [Ca2+]i in acini bathed in control medium and in Na(+)-free media with either NMDG or Li+ as the Na+ substitute. 9. Experiments in which external Ca2+ was reduced to low levels (by the addition of EGTA) just prior to addition of ACh showed that ACh released intracellular Ca2+ stores under both control and Na(+)-free conditions. 10. In acini bathed in Na(+)-free (NMDG) solution and stimulated with ACh, re-addition of either Na+ or Li+ reduced [Ca2+]i. The reduction of [Ca2+]i on Na+ re-addition was blocked by EIPA. [Ca2+]i could also be reduced under these conditions by alkalinizing the cytosol using the weak base trimethylamine.(ABSTRACT TRUNCATED AT 400 WORDS)
Elliott
A C
AC
Department of Physiological Sciences, University of Manchester.
Lau
K R
KR
Brown
P D
PD
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D011817:Rabbits; D012469:Salivary Glands / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*
D012964:Sodium; D000109:Acetylcholine; D002118:Calcium