L-NG-nitro-arginine inhibits nicotine-induced relaxation of isolated rat duodenum.
We studied the effects of L-NG-nitro-arginine (L-NOARG), which inhibits nitric oxide (NO) biosynthesis from L-arginine, on the non-adrenergic, non-cholinergic (NANC)-mediated relaxation induced by nicotine in isolated rat duodenum. L-NOARG reduced nicotine-induced relaxation, and L-arginine prevented the inhibitory effect of L-NOARG. However, L-NOARG did not inhibit the tetrodotoxin-insensitive relaxation induced by adenosine 5'-triphosphate, alpha, beta-methyleneadenosine 5'-triphosphate, thyrotropin-releasing hormone or the calcitonin gene-related peptide. Endogenous NO thus could possibly be involved in the NANC-mediated relaxation of rat duodenum induced by nicotine.
Irie
K
K
Department of Pharmacology, Tokyo Women's Medical College, Japan.
Muraki
T
T
Furukawa
K
K
Nomoto
T
T
D000227:Adenine Nucleotides / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000494:pharmacology; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology; D004386:Duodenum / Q000187:drug effects*; D006584:Hexamethonium Compounds / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D009538:Nicotine / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D019335:Nitroarginine; D051381:Rats; D011919:Rats, Inbred Strains; D013779:Tetrodotoxin / Q000494:pharmacology; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology
D000227:Adenine Nucleotides; D006584:Hexamethonium Compounds; D019335:Nitroarginine; D013779:Tetrodotoxin; D013973:Thyrotropin-Releasing Hormone; D009538:Nicotine; D000255:Adenosine Triphosphate; D001120:Arginine; D015740:Calcitonin Gene-Related Peptide; C002630:alpha,beta-methyleneadenosine 5'-triphosphate