title: Characterization of a Cryptosporidium parvum sporozoite glycoprotein.
abstract: Polyclonal and monoclonal antibodies directed against Cryptosporidium oocysts or sporozoites were developed to identify and characterize sporozoite pellicle and apical complex antigens. A very large glycoprotein of Cryptosporidium sporozoites was identified by three monoclonal antibodies that also reacted with intracellular merozoites. The glycoprotein was also identified by polyclonal antibodies that were affinity-purified on nitrocellulose-bound recombinant proteins expressed by four lambda gtll genomic clones.
authors.affiliation: Department of Medicine, San Francisco General Hospital, CA 94110.
authors.forename: C
authors.initials: C
authors.lastname: Petersen
authors.forename: J
authors.initials: J
authors.lastname: Gut
authors.forename: R G
authors.initials: RG
authors.lastname: Nelson
authors.forename: J H
authors.initials: JH
authors.lastname: Leech
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D003458:Cryptosporidium / Q000737:chemistry* / Q000276:immunology; D005260:Female; D015698:Genomic Library; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins / Q000276:immunology / Q000302:isolation & purification*; D013170:Spores / Q000737:chemistry / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D015800:Protozoan Proteins