Determination of cytosolic ADP and AMP concentrations and the free energy of ATP hydrolysis in human muscle and brain tissues with 31P NMR spectroscopy.
Based on experimentally determined concentrations of PCr, Pi, ATP, and H+, the ADP and AMP concentrations and the free energy for the ATP hydrolysis in normal human brain and muscle were calculated assuming that the creatine kinase- and the myokinase-catalyzed reactions were in equilibrium, accounting for changes in pH and free Mg2+ ion concentration.
Roth
K
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Department of Medicine, University of California, San Francisco 94143.
Weiner
M W
MW
D000244:Adenosine Diphosphate / Q000032:analysis*; D000249:Adenosine Monophosphate / Q000032:analysis*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004734:Energy Metabolism; D006801:Humans; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D009132:Muscles / Q000378:metabolism*
D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate