Proteinases of the mammary gland: developmental regulation in vivo and vectorial secretion in culture.
The extracellular matrix (ECM) is an important regulator of mammary epithelial cell function both in vivo and in culture. Substantial remodeling of ECM accompanies the structural changes in the mammary gland during gestation, lactation and involution. However, little is known about the nature of the enzymes and the processes involved. We have characterized and studied the regulation of cell-associated and secreted mammary gland proteinases active at neutral pH that may be involved in degradation of the ECM during the different stages of mammary development. Mammary tissue extracts from virgin and pregnant CD-1 mice resolved by zymography contained three major proteinases of 60K (K = 10(3) Mr), 68K and 70K that degraded denatured collagen. These three gelatinases were completely inhibited by the tissue inhibitor of metalloproteinases. Proteolytic activity was lowest during lactation especially for the 60K gelatinase which was shown to be the activated form of the 68K gelatinase. The activated 60K form decreased prior to parturition but increased markedly after the first two days of involution. An additional gelatin-degrading proteinase of 130K was expressed during the first three days of involution and differed from the other gelatinases by its lack of inhibition by the tissue inhibitor of metalloproteinases. The activity of the casein-degrading proteinases was lowest during lactation. Three caseinolytic activities were detected in mammary tissue extracts. A novel 26K cell-associated caseinase--a serine arginine-esterase--was modulated at different stages of mammary development. The other caseinases, at 92K and a larger than 100K, were not developmentally regulated. To find out which cell type produced the proteinases in the mammary gland, we isolated and cultured mouse mammary epithelial cells. Cells cultured on different substrata produced the full spectrum of gelatinases and caseinases seen in the whole gland thus implicating the epithelial cells as a major source of these enzymes. Analysis of proteinases secreted by cells grown on a reconstituted basement membrane showed that gelatinases were secreted preferentially in the direction of the basement membrane. The temporal pattern of expression of these proteinases and the basal secretion of gelatinases by epithelial cells suggest their involvement in the remodelling of the extracellular matrix during the different stages of mammary development and thus modulation of mammary cell function.
Talhouk
R S
RS
Division of Cell and Molecular Biology, Lawrence Berkeley Laboratory, Berkely, CA 94720.
Chin
J R
JR
Unemori
E N
EN
Werb
Z
Z
Bissell
M J
MJ
D000818:Animals; D002364:Caseins / Q000378:metabolism; D002478:Cells, Cultured; D004586:Electrophoresis / Q000379:methods; D010450:Endopeptidases / Q000502:physiology*; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D005109:Extracellular Matrix / Q000201:enzymology*; D005260:Female; D005780:Gelatin / Q000378:metabolism; D007774:Lactation / Q000502:physiology; D008321:Mammary Glands, Animal / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy
D002364:Caseins; D005780:Gelatin; D010450:Endopeptidases