title: Retinol deficient diet decreases binding activity of testicular fatty acid-binding protein.
abstract: Fatty acid-binding protein (FABP) activity was determined in livers and testes of retinol sufficient, retinol deficient, and also in a group of retinol deficient rats, which were refed 100 micrograms of retinyl acetate for four hours. [14C]palmitoyl CoA binding assay was used to estimate the binding activity of FABP. In retinol sufficient and retinol deficient groups, liver FABP bound 431 and 446 pmol [14C]palmitoyl CoA/mg cytosolic protein respectively. In contrast, testicular FABP bound 254 and 139 pmol [14C]palmitoyl CoA/mg cytosolic protein in retinol sufficient and deficient groups. Retinol deficiency caused a tissue specific change in testicular FABP and not in liver FABP. Liver and testicular FABP's remained unchanged after four hour refeeding of retinol to retinol deficient rats.
authors.affiliation: Department of Biochemistry, Vanderbilt University, Nashville, TN 37232.
authors.lastname: Riaz-ul-Haq
authors.forename: Z
authors.initials: Z
authors.lastname: Haq
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D004032:Diet; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000378:metabolism*; D014802:Vitamin A Deficiency / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins