[Isolation of the alkaline phosphatase from the intestinal contents of common seal by immobilized monoclonal antibodies].
It is shown that monoclonal antibodies against the alkaline phosphatase of the Greenland seal interact with the alkaline phosphatase of the bowels contents of adult common seal (Phoca vitulina larga). The purified antibodies were covalently bound with BrCn-activated sepharose 4B and used as an immunosorbent for purification of the alkaline phosphatase of the bowels contents. The specific activity of the purified is equal to 7300 units per 1 mg of protein.
Sakharov
I Iu
IIu
D000469:Alkaline Phosphatase / Q000302:isolation & purification*; D000818:Animals; D000911:Antibodies, Monoclonal; D007163:Immunosorbent Techniques; D007422:Intestines / Q000201:enzymology*; D046048:Seals, Earless / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000469:Alkaline Phosphatase
Vydelenie shchelochnoĭ fosfatazy iz soderzhimogo kishechnika tiulenia obyknovennogo na immobilizovannykh monoklonal'nykh antitelakh.