title: [Effect of the composition of a nutrient medium on growth and synthesis of ergot alkaloids by Claviceps purpurea in the saprotrophic culture].
abstract: The impact of changes in the ratio of the medium-668 components on growth and synthesis of ergot alkaloids in saprotrophic cultures was studied with mathematical design of the experiment. Medium-668 used for cultivation of strain VNIIA-312A-producing peptide +ergot alkaloids was shown to be balanced with respect to the ratio of all the medium components. An important role of phosphate in control of culture growth and alkaloids synthesis was elucidated. It was demonstrated that by changing the ratio of the medium components it was possible to control accumulation and excretion of the alkaloids which permitted development of the conditions required for product isolation.
authors.forename: M A
authors.initials: MA
authors.lastname: Sarkisova
authors.forename: V M
authors.initials: VM
authors.lastname: Fishman
mesh_terms: D002951:Citrates / Q000008:administration & dosage / Q000494:pharmacology*; D019343:Citric Acid; D002967:Claviceps / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media; D004876:Ergot Alkaloids / Q000378:metabolism*; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology*; D006133:Growth Substances / Q000008:administration & dosage / Q000494:pharmacology*; D066298:In Vitro Techniques; D010710:Phosphates / Q000008:administration & dosage / Q000494:pharmacology*; D013395:Sucrose / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D002951:Citrates; D003470:Culture Media; D004876:Ergot Alkaloids; D006133:Growth Substances; D010710:Phosphates; D019343:Citric Acid; D013395:Sucrose; D005947:Glucose
vernacular_title: Vliianie sostava pitatel'noĭ sredy na rost i sintez alkaloidov sporyn'i v saprotrofnoĭ kul'ture.