Generation of antibodies against human hsp27 and murine hsp25 by immunization with a chimeric small heat shock protein.
A hybrid protein containing the N-terminal part of the murine stress protein hsp25 (amino acids 1 to 110) and the C-terminal part of the human stress protein hsp27 (amino acids 111 to 208) was expressed in E. coli using a T7 polymerase/promoter system. The recombinant hybrid protein was purified and used for immunization of rabbits. In contrast to immunization experiments using hsp25 and hsp27 alone, immunization with the hybrid protein hsp25/27 leads to antibodies which can be used for detection of both hsp25 and hsp27.
Engel
K
K
Institute of Molecular Biology, Department of Cell Physiology, Berlin-Buch, FRG.
Knauf
U
U
Gaestel
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics / Q000276:immunology; D002678:Chimera; D003429:Cross Reactions; D004247:DNA / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007114:Immunization; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D014407:Tumor Cells, Cultured
D006360:Heat-Shock Proteins; D011994:Recombinant Proteins; D004247:DNA