Involvement of the endogenous lectin CSL in adhesion of Chinese hamster ovary cells.
Immunochemical localization of an endogenous mannose-binding protein, the cerebellar soluble lectin (CSL; Zanetta et al., J. Neurochem. 49, 1250-1257 (1987)), in Chinese hamster ovary cells indicated its high concentration in areas of contact between cells. This suggested its role in cell adhesion. The pattern of staining differed significantly in the cells cultured in suspension from that grown as monolayer. In cells maintained for a short time as suspension, the extracellular CSL immunoreactivity was found mainly in close apposition to the plasma membrane including contact areas. In cells cultured as monolayer, extracellularly, the lectin was found both at the cell surface and in a 75-nm thick layer between two cells, apparently adhering to the cell surface through bridges. Endogenous glycoprotein ligands of CSL were present in the cultures of CHO cells, both as membrane-bound glycoproteins and as glycoprotein ligands soluble in the presence of mannose in the absence of detergent. The lectin CSL induced adhesion between these cells as evident by low concentration of anti-CSL Fab fragments inhibiting such adhesion. These data suggested that adhesion between CHO cells occurs, in part, through a glycobiological recognition system involving CSL. This mechanism should be taken into account for the interpretation of experiments of transfection in CHO cells of the genes of glycoproteins involved in cell adhesion.
Lehmann
S
S
Laboratoire de Neurobiologie Moléculaire des Interactions Cellulaires CNRS-UPR A0416, Centre de Neurochimie du CNRS, Strasbourg, France.
Kuchler
S
S
Badache
A
A
Zaepfel
M
M
Meyer
A
A
Zanetta
J P
JP
D000818:Animals; D016466:CHO Cells / Q000502:physiology* / Q000648:ultrastructure; D002448:Cell Adhesion / Q000502:physiology*; D002449:Cell Aggregation / Q000502:physiology*; D006224:Cricetinae; D006023:Glycoproteins / Q000502:physiology*; D007124:Immunoenzyme Techniques; D007140:Immunoglobulin Fab Fragments; D007150:Immunohistochemistry; D037102:Lectins / Q000502:physiology* / Q000648:ultrastructure; D008024:Ligands; D016253:Microscopy, Immunoelectron
D006023:Glycoproteins; D007140:Immunoglobulin Fab Fragments; D037102:Lectins; D008024:Ligands; C059525:cerebellar soluble lectin