Visualization of proteases within a complex sample following their selective retention on immobilized bacitracin, a peptide antibiotic.
A method for the visualization of individual proteases within a complex biological sample is described. In a single chromatographic step, proteases can be separated from other biomolecules by selective binding to immobilized bacitracin, a peptide antibiotic. Following desorption, proteases may be separated by SDS-polyacrylamide gel electrophoresis. The application of this method is presented in the visualization of proteases secreted by the fungus Aspergillus niger.
van Noort
J M
JM
TNO-Medical Biological Laboratory, Rijswijk, The Netherlands.
van den Berg
P
P
Mattern
I E
IE
D001234:Aspergillus niger / Q000201:enzymology / Q000378:metabolism; D001414:Bacitracin / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000737:chemistry* / Q000172:deficiency / Q000302:isolation & purification; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D012685:Sepharose
D010455:Peptides; D001414:Bacitracin; D012685:Sepharose; D010450:Endopeptidases