Preparation of oligonucleotide-peptide conjugates.
A procedure for preparing oligonucleotide-peptide conjugates is presented. It is based on appending a maleimide group to the oligonucleotide for selective coupling to the thiol side chain of a cysteine residue in the peptide. A convenient chromatographic purification procedure, based on Fmoc-on/Fmoc-off, is described.
Tung
C H
CH
Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, New Jersey 08854.
Rudolph
M J
MJ
Stein
S
S
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000737:chemistry; D005449:Fluorenes / Q000737:chemistry; D008301:Maleimides / Q000737:chemistry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry
D000596:Amino Acids; D005449:Fluorenes; D008301:Maleimides; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D009841:Oligonucleotides; D010455:Peptides; D013438:Sulfhydryl Compounds; D003545:Cysteine