title: Partial purification of alpha-hemolysin in Escherichia coli.
abstract: The present paper describes isolation and purification of alpha-hemolysin of Escherichia coli. The optimum production medium was found to be the Todd-Hewitt broth. Out of thirteen fractions obtained after separation on Sephadex G-200, two fractions possessed the highest relative specific activity.
authors.affiliation: Department of Microbiology, Faculty of Medicine, P.J. Safárik University, Kosice, Czechoslovakia.
authors.forename: F
authors.initials: F
authors.lastname: Nistiar
authors.forename: V
authors.initials: V
authors.lastname: Janigová
authors.forename: J
authors.initials: J
authors.lastname: Durovicová
authors.forename: H
authors.initials: H
authors.lastname: Puzová
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel; D003470:Culture Media; D004912:Erythrocytes / Q000382:microbiology; D004926:Escherichia coli / Q000737:chemistry* / Q000254:growth & development; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli