Monoclonal antibody against platelet thrombospondin decreases erythrocyte aggregation rate.
The effect of thrombospondin, a major glycoprotein in the platelet alpha-granule, on the erythrocyte aggregation rate was investigated. Venous blood was sampled from 8 healthy male volunteers and anticogulated with 1.1 mg/ml EDTA(K2). The erythrocyte aggregation rate of each blood sample was measured with a whole-blood erythrocyte aggregometer before and after incubation with murine monoclonal antibody against human platelet thrombospondin. After 15 min incubation, the erythrocyte aggregation rate exhibited a significant decrease to 0.055 +/- 0.022/s, representing 71.9 +/- 8.7% of the control value (0.075 +/- 0.028/s) (p less than 0.0005). The results obtained suggest that thrombospondin may participate in the control of erythrocyte aggregability in the circulating blood.
Ohta
K
K
Department of Neurology, Keio University School of Medicine, Tokyo, Japan.
Fukuuchi
Y
Y
Tomita
M
M
Tanahashi
N
N
Matsuoka
S
S
Takeda
H
H
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology*; D004903:Erythrocyte Aggregation / Q000276:immunology*; D006801:Humans; D008297:Male; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*; D012212:Rheology; D019699:Thrombospondins
D000911:Antibodies, Monoclonal; D010980:Platelet Membrane Glycoproteins; D019699:Thrombospondins