[The development of and experimentation with a kit for the identification and rapid biotyping of H. influenzae and H. parainfluenzae].
The paper reports on the drawing up and experimentation of a kit of media for identification in liquid media and biotyping of H. influenza and H. parainfluenza. The kit is made up of 14 components, out of which the following were prepared: basic broth, X V broth, V broth, X broth, red-phenol broth, and XV factors + ribose, xylose and saccharose, urea substrate. Moeller medium with XV, with and without ornithine, covered with paraffin oil after impregnation. In the XV broth and the broth with red phenol the XV factors and saccharose, the bands for indole and H2S were put after impregnation with bacterial culture. On these media, 175 strains of Haemophilus were identified and biotyped. 109 of them were H. influenzae and 66 H. parainfluenzae, when satellitism was used. Identification in liquid media showed that 5.5% of the H. influenza strains were H. parainfluenza and 10.6% of the H. parainfluenza strains were H. influenza. Finally, 110 strains were H. influenzae and 65 H. parainfluenzae. The components of the kit permitting identification and biotyping of H. influenzae and H. parainfluenzae are: basic broth with red phenol, X broth, XV factors supplement, V supplement, mono- and bipotassium phosphate solution, 20% urea solution, 20% saccharose, 20% ornithine, Moeller medium, bands for indole and paraffine oil.
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Institutul Cantacuzino.
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Caplan
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D015373:Bacterial Typing Techniques / Q000295:instrumentation; D003470:Culture Media; D005069:Evaluation Studies as Topic; D006190:Haemophilus / Q000145:classification / Q000302:isolation & purification*; D006192:Haemophilus Infections / Q000175:diagnosis; D006193:Haemophilus influenzae / Q000145:classification / Q000302:isolation & purification*; D006801:Humans; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic
D003470:Culture Media; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic
Elaborarea şi experimentarea unei truse pentru identificarea şi biotiparea rapită a H. influenzae şi H. parainfluenzae.