Carbohydrate antigen of serotype g "Streptococcus milleri": immunochemical characterization.
Cell wall carbohydrate antigen of the serotype g "Streptococcus milleri" was extracted with cold trichloroacetic acid from purified cell walls of the type strain K1K. The extracts were then purified by a DEAE-Sephadex A-25 column, followed by a Sephadex G-100 column. The immunoelectrophoresis revealed that the serotype g carbohydrate antigen preparation displayed a single precipitin band against the crude anti-K1K serum. The purified type g antigen consisted of rhamnose, galactose, glucose and galactosamine in a ratio of 1.3:3.8:1.0:2.5. The quantitative precipitin inhibition test with various haptens indicated that galactosamine is a major immunodeterminant of the type g-specific antigen.
Inoue
M
M
Department of Preventive Dentistry, Kagoshima University Dental School, Japan.
Yakushiji
T
T
Konagawa
R
R
D002473:Cell Wall; D002852:Chromatography, Ion Exchange; D003773:Dental Plaque / Q000382:microbiology; D005688:Galactosamine / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006801:Humans; D007122:Immunoelectrophoresis; D007150:Immunohistochemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D012210:Rhamnose / Q000032:analysis; D012703:Serotyping; D013291:Streptococcus / Q000276:immunology*
D011135:Polysaccharides, Bacterial; D005688:Galactosamine; D005947:Glucose; D012210:Rhamnose; D005690:Galactose