Effects of perfluorodecalin and perfluorooctylbromide emulsions on peak contractile force and ATP levels in the guinea pig left atrium.
The isolated, electrically-driven, guinea pig left atrium was used to study the ability of two perfluorocarbon emulsions to prevent anaerobic hypofunction in the myocardium. A 20% perfluorodecalin (PFD) emulsion maintained peak tension at 80% of aerated levels for more than 20 minutes. Emulsions of perfluorooctylbromide (PFOB) ranging from 25 to 100% produced a similar result, except that the 25% emulsion could not maintain contractions for the entire time period. Peak tension of atria bathed in K-H solution decreased to less than 50% over the same time period. Both 20% PFD and 100% PFOB maintained myocardial ATP levels at pre-hypoxic levels for at least twenty minutes after aeration was terminated. Unaerated atria, bathed in Krebs-Henseleit solution only, exhibited a significant decline in tissue ATP levels at this time. It appears that perfluorocarbon emulsions may delay oxygen desaturation and thereby protect cardiac tissue from ATP depletion and impaired cardiac function associated with hypoxia. This tissue preparation was found to be very useful for determining the efficacy of potential oxygen carriers.
Eyer
C L
CL
School of Pharmacy, University of Montana, Missoula 59812.
Olszanski
A J
AJ
Smith
J R
JR
Anderson
A C
AC
Irem
S O
SO
D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004655:Emulsions; D005466:Fluorocarbons / Q000494:pharmacology*; D006168:Guinea Pigs; D006325:Heart Atria / Q000187:drug effects*; D006842:Hydrocarbons, Brominated; D066298:In Vitro Techniques; D009200:Myocardial Contraction / Q000187:drug effects*; D010100:Oxygen / Q000032:analysis / Q000494:pharmacology
D001802:Blood Substitutes; D004655:Emulsions; D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; C008805:perfluorodecalin; D000255:Adenosine Triphosphate; C003072:perflubron; D010100:Oxygen