Gene regulation in megakaryocyte.
Analysis of the mechanisms which control the differentiation of the megakaryocytic lineage is a major commitment to understand the production of circulating blood platelets. One way to approach this question is to examine the promoter domain of a marker gene which is expressed exclusively in the megakaryocytic lineage and at an early stage of the differentiation process. For this purpose the gene coding for the platelet specific glycoprotein IIb was isolated and its promoter was analysed. This promoter contains positive and negative DNA responsive elements that are responsible for the cell specific expression of the gene. With this promoter region it is now possible to direct the expression of heterologous genes in vivo using the transgenic approach.
Marguerie
G
G
Institut National de la Santé et de la Recherche Médicale, U217, Laboratoire d'Hématologie, Grenoble, France.
Denarier
E
E
Prandini
M H
MH
Martin
F
F
Schweitzer
A
A
Thevenon
D
D
Roullot
V
V
Berthier
R
R
Uzan
G
G
D015415:Biomarkers / Q000097:blood; D005786:Gene Expression Regulation / Q000502:physiology*; D006133:Growth Substances / Q000502:physiology; D006801:Humans; D008533:Megakaryocytes / Q000502:physiology*; D010980:Platelet Membrane Glycoproteins / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics
D015415:Biomarkers; D006133:Growth Substances; D010980:Platelet Membrane Glycoproteins