Immunocytochemical localization of calbindin-D28k, calbindin-D9k and parvalbumin in rat kidney.
Possible sites involved in active Ca2+ transport were traced by means of immunocytochemical detection of calcium-binding proteins (CaBP) in the mammalian kidney. Antisera were raised in rabbits against calbindin-D28k from chick kidney and calbindin-D9k from bovine intestine and parvalbumin from rabbit muscle. In the rat kidney, parvalbumin and calbindin-D9k were co-localized in the loops of Henle and distal convoluted tubule. In the collecting duct their presence was restricted to the intercalated cells. In all responsive cells parvalbumin and calbindin-D9k were present exclusively along the basolateral membrane. Calbindin-D28k was only present in the outer part of the cortex, where it was localized in the distal convoluted tubule and in the connecting tubule. In these cells calbindin-D28k was evenly distributed through the cytosol. Calbindin-D28k, unlike parvalbumin and calbindin-D9k, could not be demonstrated in the loops of Henle or collecting duct.
Bindels
R J
RJ
Department of Physiology, University of Nijmegen, The Netherlands.
Hartog
A
A
Timmermans
J A
JA
van Os
C H
CH
D000818:Animals; D064092:Calbindin 1; D064026:Calbindins; D002118:Calcium / Q000378:metabolism; D007150:Immunohistochemistry; D007668:Kidney / Q000737:chemistry*; D008297:Male; D010320:Parvalbumins / Q000032:analysis* / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D064030:S100 Calcium Binding Protein G / Q000032:analysis* / Q000276:immunology
C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G; C576508:S100g protein, rat; D002118:Calcium