Growth kinetics of Bu3 cells secreting HBsAg in microcarrier culture.
The genetically engineered mammalian cell (Bu3) secreting Hepatitis B surface antigen (HBsAg) was cultured on microcarriers semi-continuously. The growth model of Bu3 cells propagating on microcarriers, glucose consumption and lactate accumulation models were established. It is illustrated that the technology of anchorage-dependent Bu3 cell culture on microcarriers will give high yield and high HBsAg expression.
Chen
Z
Z
Research Institute of Biochemical Engineering, East China University of Chemical Technology, Shanghai.
Shi
Y
Y
Yuan
S
S
Ding
J
J
D000818:Animals; D002455:Cell Division; D002460:Cell Line / Q000378:metabolism; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods*; D004274:DNA, Recombinant; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics
D004274:DNA, Recombinant; D006514:Hepatitis B Surface Antigens