Stimulation of human neutrophil phagocytosis of Candida albicans by a beta-glucan synthase inhibitor, cilofungin.
The effect of a lipopeptide antifungal agent, cilofungin, on serum opsonization and phagocytosis of Candida albicans yeast phase cells in human neutrophil monolayer assays was investigated. Simultaneous addition of fungicidal concentrations of cilofungin did not enhance or inhibit phagocytosis of C. albicans. Pretreatment of Candida blastospores with cilofungin in the absence of serum complement for 1 h did not affect phagocytosis. However, pretreatment of blastospores with cilofungin and complement promoted a significant increase in ingestion. Pretreatment of neutrophils with cilofungin in serum-free media did not affect neutrophil viability. In contrast, pre-exposure of neutrophils to cilofungin in the presence of complement inhibited ingestion of blastospores.
Richardson
M D
MD
Department of Dermatology, University of Glasgow, U.K.
Smyth
L A
LA
Shankland
G S
GS
D000704:Analysis of Variance; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000276:immunology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003165:Complement System Proteins / Q000494:pharmacology; D004357:Drug Synergism; D054714:Echinocandins; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D009895:Opsonin Proteins / Q000187:drug effects; D010455:Peptides / Q000494:pharmacology; D010456:Peptides, Cyclic; D010587:Phagocytosis / Q000187:drug effects*
D000935:Antifungal Agents; D054714:Echinocandins; D009895:Opsonin Proteins; D010455:Peptides; D010456:Peptides, Cyclic; C042101:cilofungin; D003165:Complement System Proteins