Dynamics of 125I-labelled horse serum albumin presentation in vivo in guinea pigs.
Low antibody production in guinea-pig sera was determined by passive hemagglutination after 125I-labelled horse serum albumin (HoSA) injection. The appearance of radiolabelled HoSA on T cells, B cells and monocytes/macrophages (Mo) of guinea pigs was detected and followed as a function of time. The radioactivity peaks appeared first on B cells and Mo, later on the T cells. The circulating T lymphocytes contained labelled antigen 7 days after the injection while T and B lymphocytes in the spleen preserved their radioactivity 15 and 20 days later. Helper, suppressor and effector T cells were able to fix 125I-HoSA, this was shown by autoradiography using monoclonal antibodies 4 days following the antigen injection.
Pongracz
J
J
Department of Pathophysiology, University Medical School of Debrecen, Hungary.
Csaba
B
B
D000818:Animals; D000917:Antibody Formation / Q000528:radiation effects*; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology / Q000528:radiation effects; D006168:Guinea Pigs; D006736:Horses; D007457:Iodine Radioisotopes / Q000008:administration & dosage*; D009000:Monocytes / Q000276:immunology / Q000528:radiation effects; D010047:Ovalbumin / Q000276:immunology; D012709:Serum Albumin / Q000008:administration & dosage / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology / Q000528:radiation effects
D007457:Iodine Radioisotopes; D012709:Serum Albumin; D010047:Ovalbumin