Antidiuretic response: what markers for water channel components?
Antidiuretic hormone increases the water permeability of its target epithelial tissues by triggering the insertion into the apical cell membrane of aggregated intramembrane particles that contain channels specific for water. Little is known about the chemical composition of these membrane particles and of the water channel components. Present work describes a procedure for obtaining selected antibodies that specifically recognize ADH-induced components of the apical membrane in the amphibian urinary bladder epithelial cells.
Calamita
G
G
Istituto di Fisiologia Generale, Università di Bari, Italia.
Valenti
G
G
Frigeri
A
A
Svelto
M
M
Bourguet
J
J
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015415:Biomarkers; D001834:Body Water / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D005614:Freeze Fracturing; D007163:Immunosorbent Techniques; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D016253:Microscopy, Immunoelectron / Q000379:methods*; D011898:Ranidae / Q000378:metabolism; D011975:Receptors, Mitogen / Q000032:analysis; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D014667:Vasopressins / Q000494:pharmacology / Q000502:physiology*; D014909:Wheat Germ Agglutinins / Q000378:metabolism
D000906:Antibodies; D015415:Biomarkers; D008565:Membrane Proteins; D011975:Receptors, Mitogen; D014909:Wheat Germ Agglutinins; C027503:wheat germ agglutinin receptor; D014667:Vasopressins