The production of a bispecific anti-CEA, anti-hapten (4-amino-phthalate) hybrid-hybridoma.
A standard hybridoma fusion technique was used to produce a monoclonal antibody capable of binding both carcinoembryonic antigen (CEA) and the hapten 4-amino-phthalate. A hypoxanthine-aminopterin-thymidine (HAT) sensitive anti-CEA hybridoma and KLH-phthalate immunized spleen cells were hybridized to yield clones producing bispecific monoclonal antibodies. The desired bispecific antibody was identified using both enzyme-linked immunosorbent assay (ELISA) and radio-immunoassay. The resultant hybrid-hybridoma or "tridoma" was subcloned and expanded to yield a stable population. Bifunctional antibody was then isolated from the various possible recombinants by ion exchange chromatography. This general method may be used to produce bispecific monoclonals against a wide variety of tumor associative antigens and reagents for immunodetection or treatment.
Lloyd
F
F
Roswell Park Memorial Institute, Buffalo, New York.
Goldrosen
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D006241:Haptens / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology / Q000502:physiology*; D066298:In Vitro Techniques; D051379:Mice; D010795:Phthalic Acids / Q000276:immunology*; D013154:Spleen / Q000166:cytology* / Q000502:physiology
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D006241:Haptens; D010795:Phthalic Acids; C073607:4-aminophthalate