[The effect of recombinant human interleukin-1 beta on the repopulation of bone-marrow colony-forming cells in mice subjected to the action of radiation and burns].
The radioprotective and restorative (therapeutic) effects of human recombinant interleukin-1 beta (IL-1 beta) on the population of bone marrow CFU-S of mice, subjected to either sublethal doses of ionising irradiation itself or the same irradiation in combination with thermal burn, are investigated. Both the effects of the agent are registered under both in vitro and in vivo irradiation in semi-, syn- and allogeneic animals. If the irradiation was combined with thermal burn, the "therapeutic" effect of the agent was demonstrated at irradiation dose equal to 3.06 Gy rather than to 6.12 Gy. If the bone marrow cells were irradiated in vitro in dose 3.06 Gy with the following heat shock at 42 degrees C for 10-20 min, the "therapeutic" effect of IL-1 beta was seen only if it was added to cells before rather than after irradiation. The radioprotective effect of IL-1 beta is maintained under in vitro, as well as in vivo conditions in the allogeneic system of transplantation of the CBA donor bone marrow to the C57BL mice.
Alekseeva
L V
LV
Izotova
N I
NI
Ketlinskiĭ
S A
SA
Pereverzev
A E
AE
Simbirtsev
A S
AS
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D016026:Bone Marrow Transplantation; D002056:Burns / Q000188:drug therapy* / Q000473:pathology; D003114:Colony-Forming Units Assay; D004353:Drug Evaluation, Preclinical; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D006801:Humans; D007375:Interleukin-1 / Q000627:therapeutic use*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011833:Radiation Injuries, Experimental / Q000188:drug therapy* / Q000473:pathology; D011837:Radiation-Protective Agents / Q000627:therapeutic use*; D011994:Recombinant Proteins / Q000627:therapeutic use; D013154:Spleen / Q000187:drug effects / Q000473:pathology
D007375:Interleukin-1; D011837:Radiation-Protective Agents; D011994:Recombinant Proteins
Vliianie rekombinantnogo interlekina-1 beta cheloveka na repopuliatsiiu kostnomozgovykh kolonieobrazuiushchikh kletok u mysheĭ, podvergnutykh deĭstviiu radiatsii i ozhoga.