Stable lyophilized reagents for the serum ferritin assay.
Two monoclonal antibodies to human ferritin, including one that was coupled to horseradish peroxidase, were lyophilized. These reagents show little loss of activity on reconstitution and demonstrate acceptable stability in the accelerated degradation test. When applied in a simple ELISA for the assay of serum ferritin along with the WHO standard for serum ferritin (80/602) they provide a robust assay with standardized reagents which is potentially suitable for use as a reference assay.
Worwood
M
M
Department of Haematology, University of Wales College of Medicine, Heath Park, Cardiff.
Thorpe
S J
SJ
Heath
A
A
Flowers
C H
CH
Cook
J D
JD
D000911:Antibodies, Monoclonal / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005293:Ferritins / Q000097:blood* / Q000276:immunology; D005612:Freeze Drying; D006735:Horseradish Peroxidase; D006801:Humans; D007391:International Cooperation; D012015:Reference Standards
D000911:Antibodies, Monoclonal; D005293:Ferritins; D006735:Horseradish Peroxidase