[Biochemical and molecular biological aspects of stomach cancer development in human and animal].
Expression of protooncogenes c-myc, N-myc, c-fos, Ha-ras 1, Ki-ras 2, yes, abl, src, N-ras, met and mos was studied in human gastric tumors and in rat gastric mucosal membrane during gastric carcinogenesis induced in rats by means of N-methyl-N'-nitro-N-nitroso guanidine (MNNG). Elevated expression of protooncogenes c-myc, c-fos, Ha-ras 1, Ki-ras 2, N-myc and Raf 1 was observed in carcinomas of human stomach. Amplification of Ha-ras 1 protooncogene was found in the human gastric tumor and metastasis. Point mutation was not detected in 12 the codon of Ha-ras I protooncogene. Expression of these protooncogenes was not altered during gastric carcinogenesis induced by MNNG in rats. However, within early steps of cancerogenesis (9 days, 3 months) amplification of ribosomal genes occurred in rat gastric mucosal membrane and in adenocarcinoma developed, while the tumor growth was accompanied by activation of mitochondrial genes.
Khanson
K P
KP
Kalinovskiĭ
V P
VP
Novikov
L B
LB
Fedorov
S N
SN
D000818:Animals; D015152:Blotting, Northern; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism; D005784:Gene Amplification; D015870:Gene Expression; D016259:Genes, myc; D011905:Genes, ras; D006801:Humans; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D009362:Neoplasm Metastasis; D009693:Nucleic Acid Hybridization; D011519:Proto-Oncogenes; D051381:Rats; D013274:Stomach Neoplasms / Q000139:chemically induced* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D013276:Stomach Ulcer / Q000235:genetics / Q000378:metabolism / Q000473:pathology
D004272:DNA, Mitochondrial; D008769:Methylnitronitrosoguanidine
Biokhimicheskie i molekuliarno-biologicheskie aspekty gastrokantserogeneza u cheloveka i zhivotnykh.