Expression of the fibroblast growth factor-5 gene in the mouse embryo.
Fibroblast growth factors (FGFs) are structurally related mitogens that can regulate the differentiation of a wide variety of cells. As a step towards elucidating the developmental roles played by one of these factors, we have used in situ hybridization methods to examine expression of the murine F gf-5 gene during embryogenesis. F gf-5 RNA was detected at seven distinct sites in the developing mouse embryo: (1) postimplantation epiblast (embryonic day 5 1/4-7 1/2), (2) lateral splanchnic mesoderm (E9 1/2-10 1/2), (3) lateral somatic mesoderm (E10 1/2-12 1/2), (4) myotomes (E10 1/2-12 1/2), (5) mastication muscle (E11 1/2-14 1/2), (6) limb mesenchyme (E12 1/2-14 1/2), and (7) acoustic ganglion (E12 1/2-14 1/2). At several of these sites, expression is spatially restricted within the tissues. We offer several hypotheses regarding the roles of FGF-5 in murine development.
Haub
O
O
Columbia University College of Physicians and Surgeons, Department of Biochemistry and Molecular Biophysics, New York, NY 10032.
Goldfarb
M
M
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D003198:Computer Simulation; D005346:Fibroblast Growth Factors / Q000235:genetics*; D015870:Gene Expression / Q000502:physiology*; D005796:Genes / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D009024:Morphogenesis / Q000235:genetics; D009693:Nucleic Acid Hybridization; D015347:RNA Probes
D015347:RNA Probes; D005346:Fibroblast Growth Factors