title: Endotoxin stimulates endothelin release from cultured epithelial cells of guinea-pig trachea.
abstract: We examined the release of endothelin from cultured epithelial cells of guinea-pig trachea in response to treatment with endotoxin, using a sandwich-enzyme immunoassay. Cultured epithelial cells released endothelin in a time-dependent fashion (3, 6, 12, 24 h), and endotoxin (4-40 micrograms/ml) significantly increased endothelin release. Endotoxin (4-10 micrograms/ml) showed no cytotoxicity against epithelial cells. These results suggest that guinea-pig airway epithelial cells are capable of producing endothelin and this peptide may be related to the pathophysiological effects of endotoxin.
authors.affiliation: Division of Pulmonary Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ninomiya
authors.forename: Y
authors.initials: Y
authors.lastname: Uchida
authors.forename: Y
authors.initials: Y
authors.lastname: Ishii
authors.forename: A
authors.initials: A
authors.lastname: Nomura
authors.forename: M
authors.initials: M
authors.lastname: Kameyama
authors.forename: M
authors.initials: M
authors.lastname: Saotome
authors.forename: T
authors.initials: T
authors.lastname: Endo
authors.forename: S
authors.initials: S
authors.lastname: Hasegawa
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016232:Endothelins / Q000096:biosynthesis / Q000378:metabolism*; D004731:Endotoxins / Q000494:pharmacology*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D006168:Guinea Pigs; D013268:Stimulation, Chemical; D014132:Trachea / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D016232:Endothelins; D004731:Endotoxins