The large-scale isolation of bryostatin 1 from Bugula neritina following current good manufacturing practices.
A novel process was designed for the large-scale isolation of bryostatin 1 from the bryozoan Bugula neritina L. in order to obtain multigram quantities of highly pure material for formulation studies, preclinical toxicology, and clinical trials in cancer patients. Multigram quantities of bryostatin 1 were obtained from a collection of approximately 10,000 gallons of wet animal. A phorbol dibutyrate (PDBu) receptor binding assay and hplc with photodiode array detection were used for the design, validation, and control of the isolation process.
Schaufelberger
D E
DE
Chemical Synthesis and Analysis Laboratory, PRI/DynCorp, National Cancer Institute--Frederick Cancer Research and Development Center, Maryland 21702.
Koleck
M P
MP
Beutler
J A
JA
Vatakis
A M
AM
Alvarado
A B
AB
Andrews
P
P
Marzo
L V
LV
Muschik
G M
GM
Roach
J
J
Ross
J T
JT
D000818:Animals; D054713:Bryostatins; D002013:Bryozoa / Q000737:chemistry*; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification*; D018942:Macrolides; D015394:Molecular Structure
D054713:Bryostatins; D007783:Lactones; D018942:Macrolides; C046785:bryostatin 1