Determination of picomole amounts of lipoxins C4, D4 and E4 by high-performance liquid chromatography with electrochemical detection.
A method for the sensitive determination of the sulphopeptide lipoxins (LXs) C4, D4 and E4 by high-performance liquid chromatography with subsequent electrochemical detection is described. The best results were obtained when the analysis was carried out with the solvent system methanol-water-trifluoroacetic acid (66:34:0.008, v/v/v). The acquired half-wave potentials were different for all investigated compounds: +1.18 V for LXC4 +1.3 V for LXD4 and +1.25 V for LXE4. The detection limits of LXC4, LXD4 and LXE4, based on a signal-to-noise ratio of 3:1, were found to be 200-700 fmol. Although sulphopeptide lipoxins possess a high molar absorptivity, electrochemical detection still is three times more sensitive than ultraviolet detection.
Gaede
F P
FP
Pharmaceutical Institute, University of Tübingen, Germany.
Kirchner
M
M
Steinhilber
D
D
Roth
H J
HJ
D001095:Arachidonic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003545:Cysteine / Q000031:analogs & derivatives* / Q000032:analysis; D004151:Dipeptides / Q000032:analysis*; D015777:Eicosanoids; D004563:Electrochemistry; D044045:Lipoxins; D009842:Oligopeptides / Q000032:analysis*
D001095:Arachidonic Acids; D004151:Dipeptides; D015777:Eicosanoids; D044045:Lipoxins; D009842:Oligopeptides; C073550:lipoxin C4; C073551:lipoxin E4; C073549:lipoxin D4; D003545:Cysteine