title: Developmental potential of parthenogenetic cells: role of genotype-specific modifiers.
abstract: The developmental potential of parthenogenetic cells derived from different mouse strains was investigated by examining their distribution in various tissues of adult aggregation chimeras. Using GPI-1 allozymes as marker, no striking differences were observed between chimeras whose parthenogenetic cells were derived from activated oocytes isolated from females of different genetic backgrounds, (C57BL/6 x CBA/J) F1, CFLP, 129, and SWR. In all the combinations tested, parthenogenetic cells were consistently absent from skeletal muscle, but there were varying contributions to most other tissues. These results suggest that the maternal duplication of chromosomes containing imprinted gene(s) responsible for the systematic elimination of parthenogenetic cells from skeletal muscle, are not subject to a pronounced influence of genotype-specific modifiers. However, the contribution of parthenogenetic cells to the brain does appear to be influenced by strain background, since a marked improvement in the survival of CFLP, 129 and perhaps SWR parthenogenetic cells in chimeric brains was observed compared with F2 cells.
authors.affiliation: Institut für Biologie III, Freiburg, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Fundele
authors.forename: S K
authors.initials: SK
authors.lastname: Howlett
authors.forename: R
authors.initials: R
authors.lastname: Kothary
authors.forename: M L
authors.initials: ML
authors.lastname: Norris
authors.forename: W E
authors.initials: WE
authors.lastname: Mills
authors.forename: M A
authors.initials: MA
authors.lastname: Surani
mesh_terms: D000818:Animals; D002678:Chimera / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression / Q000235:genetics*; D005838:Genotype; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D010312:Parthenogenesis / Q000502:physiology*