title: The dot-ELISA test for the detection of anti-PGL-1 IgM in leprosy patients and their contacts.
abstract: 1. Since dot-ELISA has recently been reported to be a sensitive, simple and fast method, we have compared it with the conventional microplate ELISA method. Sera of 124 leprosy patients, 136 household and professional contacts, and 92 controls were tested for antibodies against a Mycobacterium leprae antigen using dot-ELISA on nitrocellulose membrane filters and microplate ELISA. 2. The sensitivity of the two techniques was similar for multibacillary patients, but dot-ELISA was less sensitive for paucibacillary patients although it was more specific (100%) than ELISA (93.4%). 3. Of 21 household contacts that gave a response by ELISA, 3 were also positive by dot-ELISA; one of these 3 developed indeterminate leprosy 12 months later and the other was diagnosed as borderline lepromatous after 28 months. 4. These data indicate that dot-ELISA has a high specificity and can be a useful tool in field evaluation.
authors.affiliation: Setor de Hanseníase, Fundação Oswaldo Cruz, Rio de Janeiro, Brasil.
authors.forename: M H
authors.initials: MH
authors.lastname: Saad
authors.forename: M A
authors.initials: MA
authors.lastname: Medeiros
authors.forename: M E
authors.initials: ME
authors.lastname: Gallo
authors.forename: P P
authors.initials: PP
authors.lastname: Gontijo-Filho
authors.forename: L S
authors.initials: LS
authors.lastname: Fonseca
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology*; D016358:Contact Tracing; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006017:Glycolipids / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007075:Immunoglobulin M / Q000032:analysis*; D007918:Leprosy / Q000276:immunology* / Q000635:transmission; D011237:Predictive Value of Tests; D012867:Skin / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D006017:Glycolipids; D007075:Immunoglobulin M; C045169:phenolic glycolipid I, Mycobacterium leprae