[The hygienic toxicological evaluation of the viral preparation Mamestrin].
The preparation "Mamestrine" developed in Bulgaria on the basis of NPV virus is meant for control of the Heliothis zea. Experiments are carried out on the toxic effect of the preparation, according to the requirements of FAO/WHO for assessment of the virus preparations and the acting in this country Bulgarian State Standards. On experimental animals (white rats of both sexes, white guinea pigs and white rabbits) are studied acute oral, dermal and inhalatory toxicity, subacute oral toxicity, dermal-irritation, eye-stimulation and skin-sensitizing activity, cytotoxicity and activity of the preparation in soils and waters. Toxicometric, integral, clinical and laboratory, biochemical and histological methods are used. Unrepeated oral application of dose 2.10(9) pol.kg-1, unrepeated dermal application of dose 1.10(9) pol.kg-1, in white rats and 0,4.10(7) pol.kg-1 in guinea pigs and 4 hrs inhalation unrepeated exposure of white rats to concentration 1.10(7) pol.m-3 provoke no signs of intoxication and lethal issue and are accepted as inoperative. During a 3-month oral application of the preparation in doses 1.10(7) and 1.10(9) pol.kg-1 are established no data for toxic effect on the organism of experimental white rats of both sexes. The preparation "Mamestrine" has no skin-irritative, eye-irritative, eye-stimulative and skin-sensitizing activity. There is no cytotoxic effect on animal (kidney of monkey) and human (carcinoma of the gullet) cellular cultures.(ABSTRACT TRUNCATED AT 250 WORDS)
Khalkova
Zh
Zh
Zaĭkov
Kh
Kh
Antov
G
G
Mikhaĭlova
A
A
Dinoeva
S
S
Stoianova
Ts
Ts
D000818:Animals; D016367:Baculoviridae; D002478:Cells, Cultured / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D006168:Guinea Pigs; D000882:Haplorhini; D006801:Humans; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D011817:Rabbits; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects
D007306:Insecticides
Khigienno-toksikologichna otsenka na virusniia preparat "Mamestrin".