Transformation and regeneration of mung bean (Vigna radiata).
The procedure relied on a protocol in which shoot organogenesis was induced on cotyledons of mung bean genotypes selected for susceptibility to agrobacterium seems to work reproducibly if not efficiently. Approximately 4-5% of the shoots produced on the kanamycin selected cotyledons are transgenic based on assays on kanamycin resistance and GUS activity. This demonstrated that transformation and regeneration in mung bean are possible. However, raising the transformed plants in field condition is yet to be perfected.
Pal
M
M
Department of Biochemistry, Bose Institute, Calcutta.
Ghosh
U
U
Chandra
M
M
Pal
A
A
Biswas
B B
BB
D007887:Fabaceae / Q000235:genetics* / Q000502:physiology; D005966:Glucuronidase / Q000235:genetics; D007613:Kanamycin Resistance / Q000235:genetics; D030821:Plants, Genetically Modified; D010946:Plants, Medicinal; D012038:Regeneration; D014170:Transformation, Genetic
D005966:Glucuronidase