Apolipoproteins in rat cerebrospinal fluid: a comparison with plasma lipoprotein metabolism and effect of aging.
Cerebrospinal fluid (CSF) apo E concentrations, determined by a sensitive sandwich ELISA, were 411.3 +/- 76.0 and 454.3 +/- 51.8 micrograms/dl (mean +/- S.D.) for young rats (8-12 weeks old, n = 7) and old rats (36-40 weeks old, n = 10), respectively. Age-related increase, which was conspicuous in serum apo E (21.2 +/- 2.4 vs 60.9 +/- 14.1 mg/dl for young and old rats, respectively), was not observed in CSF apo E. CSF apo A-I concentrations, determined by ELISA, were extremely low in the both groups (less than 10 micrograms/dl). Neither CSF apo A-I nor CSF apo E correlated to any of the plasma lipoprotein components, indicating the presence of largely independent lipoprotein metabolism in the rat central nervous system. Apo E is present in CSF in the form of apo E-rich HDL1 with particle sizes similar to those of plasma E-rich HDL1.
Chiba
H
H
Department of Laboratory Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Mitamura
T
T
Fujisawa
S
S
Ogata
A
A
Aimoto
Y
Y
Tashiro
K
K
Kobayashi
K
K
D000375:Aging / Q000097:blood / Q000134:cerebrospinal fluid*; D000818:Animals; D001053:Apolipoproteins / Q000134:cerebrospinal fluid*; D001835:Body Weight; D002784:Cholesterol / Q000097:blood / Q000134:cerebrospinal fluid; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D010743:Phospholipids / Q000097:blood / Q000134:cerebrospinal fluid; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000097:blood / Q000134:cerebrospinal fluid
D001053:Apolipoproteins; D008074:Lipoproteins; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol