title: Expression of multivalent pre-S1 antigen of hepatitis B virus in Escherichia coli (synthetic oligodeoxynucleotides, surface antigens, recombinant DNA, fusion protein, beta-galactosidase).
abstract: The nucleotide sequence encoding 30 amino acids (aa) of the pre-S1 envelope region of the human hepatitis B virus has been constructed from twenty chemically synthesized oligodeoxynucleotides by simultaneous ligation. The DNA fragment containing four repeated sequences encoding the pre-S1 region (aa 20-49) has been inserted into the lacZ gene of the plasmid pWR450.1, yielding the recombinant pWX4 plasmid. The Escherichia coli DH5 strain transformed with pWX4 produces a beta-galactosidase-[-pre-S1(20-49) x 4] fusion protein. The hybrid protein containing 127 aa of repeated pre-S1 region has been isolated from Escherichia coli as inclusion bodies and purified by anion exchange chromatography. The antigenic properties of this fusion protein were confirmed by immunoblotting with pre-S1-specific monoclonal antibodies.
authors.affiliation: Department of Biochemistry I, Medical School, Warsaw, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Sidorkiewicz
authors.forename: J
authors.initials: J
authors.lastname: Jaworski
authors.forename: G
authors.initials: G
authors.lastname: Płucienniczak
authors.forename: A
authors.initials: A
authors.lastname: Płucienniczak
authors.forename: P
authors.initials: P
authors.lastname: Stpień
authors.forename: B
authors.initials: B
authors.lastname: Kalinowska
authors.forename: S
authors.initials: S
authors.lastname: Karwowska
authors.forename: A
authors.initials: A
authors.lastname: Nowosławski
authors.forename: B
authors.initials: B
authors.lastname: Uznański
authors.forename: A
authors.initials: A
authors.lastname: Wilk
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004274:DNA, Recombinant / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D011498:Protein Precursors / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D009838:Oligodeoxyribonucleotides; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; C050806:presurface protein 1, hepatitis B surface antigen; D001616:beta-Galactosidase