[Structure of virions of the M13 phage containing chimeric B-protein molecules].
We have studied the virion structure of M13 strains (M13B1, M13BOM1, M13BOM2, M13BOL1) with chimeric variants of B-protein. Data concerning the spatial structure of chimeric B-protein molecules and their interaction with intraphage DNA were obtained. The phage contour lengths were measured under electron microscope and the DNA/protein ratios were obtained by spectrophotometry. These data testified that the insertion of foreign peptide affected neither DNA packaging nor the compactness of molecular arrangement of proteins in the virion. By linear dichroism and fluorescence spectra of phages it was determined, that the insert can influence the polarity of amino acid environment and the orientation of amino acids in the B-protein central part. It was shown by quenching of phage fluorescence by KI that the inward or outward amino acids location in the capsid is invariable. The carboxyl residues have been titrated in the phage strains by Auramine O. It was shown that there is no correlation between the number of the titrated carboxyl groups and the number of the carboxyl groups as a whole.
Kishchenko
G P
GP
Minenkova
O O
OO
Il'ichev
A I
AI
Gruzdev
A D
AD
Petrenko
V A
VA
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000378:metabolism / Q000648:ultrastructure; D002678:Chimera; D004279:DNA, Viral / Q000378:metabolism; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D011487:Protein Conformation; D013057:Spectrum Analysis; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000378:metabolism / Q000648:ultrastructure
D004279:DNA, Viral; D014760:Viral Fusion Proteins
Izuchenie struktury virionov faga M13, soderzhashchikh molekuly khimernykh B-belkov.