A simple reflectance microphotometer for dot immunobinding assay (dot-ELISA) quantification.
The use of a simple reflectance microphotometer adapted to quantify enzymatic dot immunobinding assays is described. The instrument is versatile and precise by clinical laboratory standards (i.e., 1.1% coefficient of variation for ten consecutive readings of a grey cardboard). The instrument provides a low-cost alternative to commercially available equipment for quantitation of dot-ELISA assays.
Ferreira
C S
CS
Departamento de Parasitologia, Universidade de São Paulo, Brasil.
Ferreira
M U
MU
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015151:Immunoblotting / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood; D010783:Photometry / Q000295:instrumentation*; D010963:Plasmodium falciparum / Q000276:immunology
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G