title: [Analysis of the polypeptide profile in insulin preparations using HPLC].
abstract: The purity of insulin preparations which is important during clinical administration of this hormone can be assessed in several ways. The authors suggested and tested a method for analysis of the polypeptide composition of insulin preparations which makes quantitative and qualitative evaluation of the insulin and non-insulin character possible, as well as their separation according to the shape and size of biological macromolecules by gel filtration on HPLC equipment. The described procedure makes possible a more detailed analysis of insulin preparations when the method is used in combination with electromigration and immunological estimations.
authors.affiliation: I. interní klinika 1. lékarské fakulty Univerzity Karlovy, Praha.
authors.forename: M
authors.initials: M
authors.lastname: Streda
authors.forename: K
authors.initials: K
authors.lastname: Stajner
authors.forename: M
authors.initials: M
authors.lastname: Stredová
mesh_terms: D002851:Chromatography, High Pressure Liquid; D007328:Insulin / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*
chemical_list: D007328:Insulin; D011506:Proteins
vernacular_title: Analýza polypeptidového profilu inzulínových preparátů metodou HPLC.