Suppression of steroidogenesis in mice granulosa cells by a synthetic nonapeptide fragment of human seminal plasma inhibin.
A synthetic nonapeptide, which is C-terminal sequence of 94-amino acid of prostatic inhibin peptide was tested for progesterone and estrogen secretion by mouse granulosa cell cultures. Nonapeptide suppressed the progesterone and estrogen synthesis, the magnitude of suppression was highest at 5 ng dose level for progesterone and 50 ng dose level for estradiol. The study suggests that, nonapeptide exerts its effect by impairing the binding of FSH to granulosa cell receptors.
Hurkadli
K S
KS
Institute for Research in Reproduction (ICMR), Bombay, India.
Shahid
J K
JK
Nandedkar
T D
TD
Mahale
S D
SD
Iyer
K S
KS
Sheth
A R
AR
D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D012739:Gonadal Steroid Hormones / Q000096:biosynthesis*; D006064:Gonadotropins, Equine; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D007265:Inhibins / Q000494:pharmacology*; D051379:Mice; D010446:Peptide Fragments; D011374:Progesterone / Q000096:biosynthesis
D012739:Gonadal Steroid Hormones; D006064:Gonadotropins, Equine; D010446:Peptide Fragments; D011374:Progesterone; D007265:Inhibins; D005640:Follicle Stimulating Hormone