Differential IL-6 mRNA expression by stimulated peripheral macrophages of Staggerer and Lurcher cerebellar mutant mice.
We have recently reported about a hyperexpression of interleukin-1 mRNA by stimulated peripheral macrophages of several cerebellar mutant mice exhibiting complex patterns of neuronal degeneration in the cerebellum. Interestingly, studying the staggerer mutant mice, our data showed a hyperexpression of IL-1 beta mRNA and a hyperproduction of the cytokine. The hyperexpression of IL-1 beta mRNA was observed whatever the conditions of stimulation and the stimulating agent used, a hyperexpression of IL-1 alpha and TNF alpha mRNAs was also detected. This set of data suggests a hyperexcitability state of (sg/sg) macrophages. In the present study, we examined the IL-6 mRNA expression by stimulated peripheral macrophages of two cerebellar mutant mice, the staggerer and the lurcher mutants. Our results show that IL-6 mRNA is hyperexpressed in stimulated macrophages of staggerer mutant mice. On the contrary, no hyperexpression is observed in stimulated macrophages of lurcher mutant mice. These results suggest that the neuronal degeneration affecting the cerebellum in the two mutant mice do not lead to the same immunological defect at the peripheral level.
Kopmels
B
B
Laboratoire d'Immunologie, URA 1156, Centre National de la Recherche Scientifique, Institut Gustave Roussy, Villejuif, France.
Mariani
J
J
Taupin
V
V
Delhaye-Bouchaud
N
N
Wollman
E E
EE
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives / Q000494:pharmacology; D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D015152:Blotting, Northern; D004247:DNA / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008817:Mice, Mutant Strains; D010529:Peritoneal Cavity / Q000166:cytology; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism*
D000276:Adjuvants, Immunologic; D015850:Interleukin-6; D012333:RNA, Messenger; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C033575:murabutide; D004247:DNA