Immunobiology of Clostridium perfringens type A: passive protection of NMRI mice. Western blot analysis of immunoreactive polypeptides.
Polyvalent, equine antitoxin (250 IU/mouse) passively protected NMRI mice against 7 of 9 challenge strains of Clostridium perfringens type A. Two human immunoglobulin G (IgG) preparations (Polyglobin N and Sandoglobulin) and two rabbit immune sera prepared against formalinized cells of C. perfringens were ineffective. Cell homogenates of 4 C. perfringens strains revealed more than 15 polypeptides in SDS-PAGE electropherograms; the molecular weights ranged from 138 to 14.4 kD. Normal human serum from 2 donors revealed up to 3 immunoblot-reactive antibodies against 126-, 69.2-, 49- or 14.4-kD antigens, the two rabbit immune sera showed antibodies against 4-7 distinct antigens that ranged from 138 to 28.8 kD, whereas the two human IgG preparations revealed antibodies against up to 12 antigens (range 138.7-31.6 kD) and antibodies against lower-molecular-weight (less than 24.5-kD) components. Polyvalent, equine antitoxin reacted with at least 14 polypeptides (range 136.5-31 kD) and numerous smaller (less than 24-kD) components.
Traub
W H
WH
Institut für Medizinische Mikrobiologie und Hygiene, Universität des Saarlandes, Homburg a.d. Saar, BRD.
Bauer
D
D
Wolf
U
U
D000818:Animals; D000992:Antitoxins / Q000276:immunology*; D001427:Bacterial Toxins / Q000276:immunology; D015373:Bacterial Typing Techniques; D003016:Clostridium perfringens / Q000145:classification / Q000276:immunology*; D007116:Immunization, Passive; D016756:Immunoglobulins, Intravenous; D051379:Mice; D011817:Rabbits
D000992:Antitoxins; D001427:Bacterial Toxins; D016756:Immunoglobulins, Intravenous