Activity of phenacetin O-deethylase in rat liver: effect of inducers and inhibitors in vivo.
A method for the determination of microsomal phenacetin O-deethylase activity in the rat liver was described. Optimum conditions in which phenacetin is enzymatically converted to N-acetyl-p-aminophenol were determined. The product of enzymatic reaction was acid-hydrolyzed while heating. The amount of p-aminophenol formed was determined by means of indophenol reaction. The described method was applied to assess activity of phenacetin O-deethylase in normal rats and rats treated with inducers or inhibitors of microsomal monooxygenases, phenobarbital, 3,4-benzpyrene, SKF 525-A and cobaltous chloride, respectively.
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Department of Industrial Toxicology, Medical Academy, Cracow, Poland.
D000082:Acetaminophen / Q000378:metabolism; D000627:Aminophenols / Q000378:metabolism; D000818:Animals; D019388:Cytochrome P-450 CYP1A2; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D004790:Enzyme Induction; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006868:Hydrolysis; D008099:Liver / Q000201:enzymology*; D008297:Male; D010088:Oxidoreductases / Q000032:analysis*; D010615:Phenacetin / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000627:Aminophenols; D004791:Enzyme Inhibitors; D000082:Acetaminophen; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D019388:Cytochrome P-450 CYP1A2; D010615:Phenacetin