Bombesin excites a subpopulation of 5-hydroxytryptamine-sensitive neurones in the rat dorsal raphe nucleus in vitro.
1. The effects on dorsal raphe neurones of the peptides bombesin, gastrin-releasing peptide and neuromedin B were studied using intracellular recording techniques from slices of rat brain maintained in vitro. The peptides were added to the solutions perfusing the slices. 2. The peptides bombesin, gastrin-releasing peptide and neuromedin B depolarized neurones in the dorsal raphe nucleus. The same neurones were depolarized by phenylephrine and hyperpolarized by 5-hydroxytryptamine (5-HT) but were insensitive to sulphated cholecystokinin octapeptide (CCK). 3. The responses to the peptides were not blocked by CCKA, CCKB and alpha 1-adrenoreceptor antagonists. 4. The response to the peptides persisted in the presence of tetrodotoxin (TTX) and low-calcium, high-magnesium-containing artificial cerebrospinal fluid (ACSF). 5. Under voltage clamp conditions the peptides caused a decrease in membrane conductance accompanied by an inward current. The reversal potential for the event was the same as that for 5-HT. 6. The results of the present study demonstrate that bombesin and the structurally related peptides gastrin-releasing peptide (GRP) and neuromedin B depolarized a subpopulation of raphe 5-HT neurones by acting on a postsynaptically located receptor linked to potassium channels.
Pinnock
R D
RD
Parke-Davis Research Unit, Addenbrooke's Hospital Site, Cambridge.
Woodruff
G N
GN
D000818:Animals; D001839:Bombesin / Q000494:pharmacology*; D046508:Culture Techniques; D019886:Gastrin-Releasing Peptide; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D015287:Neurokinin B / Q000031:analogs & derivatives / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects*; D010455:Peptides / Q000494:pharmacology; D011903:Raphe Nuclei / Q000187:drug effects*; D051381:Rats; D012701:Serotonin / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology
D010455:Peptides; D012701:Serotonin; D013779:Tetrodotoxin; D019886:Gastrin-Releasing Peptide; D015287:Neurokinin B; C038393:neuromedin B; D008274:Magnesium; D001839:Bombesin