title: Simplified, rapid and inexpensive extraction procedure for a high-performance liquid chromatographic method for determination of disopyramide and its main metabolite mono-N-dealkylated disopyramide in serum.
abstract: A simplified, rapid and inexpensive extraction procedure for the determination of the antiarrhythmic drug disopyramide and its main metabolite mono-N-desalkylated disopyramide in serum by high-performance liquid chromatography has been developed. The analysis uses ultraviolet detection at 254 nm, and a 5 micron reversed-phase column with a mobile phase of water-triethylamine-acetonitrile-PIC-B8 reagent. Serum extraction is performed with dichloromethane and 1 M sodium hydroxide. p-Chlorodisopyramide is used as internal standard. Recovery rates were 94.5% (S.D. 5.7%) for disopyramide, 96.8% (S.D. 2.2%) for mono-N-desalkylated disopyramide and 97.9% (S.D. 2.8%) for the internal standard.
authors.affiliation: Abteilung III, Eberhard-Karls-Universität Tübingen, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Mayer
authors.forename: B K
authors.initials: BK
authors.lastname: Krämer
authors.forename: K M
authors.initials: KM
authors.lastname: Ress
authors.forename: V
authors.initials: V
authors.lastname: Kühlkamp
authors.forename: H M
authors.initials: HM
authors.lastname: Liebich
authors.forename: T
authors.initials: T
authors.lastname: Risler
authors.forename: L
authors.initials: L
authors.lastname: Seipel
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004206:Disopyramide / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: C030438:4-(isopropylamino)-2-(2-pyridyl)-2-phenylbutyramide; D004206:Disopyramide