Effect of histamine on collagen and collagen m-RNA production in human skin fibroblasts.
Direct effects of histamine on collagenous and non-collagenous protein synthesis by human skin fibroblasts were studied. Fibroblasts derived from human skin were incubated with various concentrations of histamine. Collagen and non-collagenous protein synthesis were measured by incorporation of 3H-proline. Both collagen synthesis measured as protein-bound hydroxyproline and non-collagenous protein synthesis measured as protein-bound proline increased in the presence of histamine at concentrations of 10(1)-10(2) micrograms/ml. Total RNA was extracted and m-RNA levels of various proteins were estimated by dot blot analysis, and densitometrically quantified. The levels of alpha 1(I) collagen and beta-actin m-RNA were clearly increased at the same concentrations. m-RNA levels of alpha 1(III) collagen were also increased but the rate was lower than that of alpha 1(I) collagen. No alteration of beta-tubulin m-RNA level was observed at the same concentrations. These results demonstrate that stimulation of collagen synthesis by histamine is pretranslationally controlled.
Hatamochi
A
A
Department of Dermatology, Kawasaki Medical School, Okayama, Japan.
Ueki
H
H
Mauch
C
C
Krieg
T
T
D000199:Actins / Q000096:biosynthesis; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D014404:Tubulin / Q000096:biosynthesis
D000199:Actins; D012333:RNA, Messenger; D014404:Tubulin; D006632:Histamine; D003094:Collagen