Diagnosis of Pneumocystis carinii pneumonia by 5S ribosomal DNA amplification.
The polymerase chain reaction technique was used to detect Pneumocystis carinii by amplifying the P. carinii 5S ribosomal DNA. The efficacy and specificity of this diagnostic method is reported. Analysis of patients' sputa indicate that the method can be used on these samples for the diagnosis of P. carinii pneumonia.
Kitada
K
K
Institute of Medical Science, University of Tokyo, Japan.
Oka
S
S
Kimura
S
S
Shimada
K
K
Nakamura
Y
Y
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D000818:Animals; D001483:Base Sequence; D004271:DNA, Fungal / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000032:analysis*; D008168:Lung / Q000382:microbiology; D051379:Mice; D008969:Molecular Sequence Data; D009894:Opportunistic Infections / Q000175:diagnosis; D016377:Organ Transplantation; D011020:Pneumonia, Pneumocystis / Q000150:complications / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D012331:RNA, Fungal / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D051381:Rats
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012331:RNA, Fungal; D012341:RNA, Ribosomal, 5S