[The possible cytophotometry of nucleolar proteins. The prospects for research on the status of the nucleolus apparatus].
Possibility of cytophotometry application was established for the acid nucleolar proteins reacting with silver nitrate. The increase in transcription in the Purkinje neurons results in the expansion of the Ag-proteins areas, whereas their amount may be not increased. The amount and area of the Ag-proteins double in hepatocytes through polyploidization, but the number of nucleoli does not correspond to the gene dosage. This lack of correspondence was also revealed in micronucleoli. The number of nucleoli is not similar in the nuclei of some binuclear hepatocytes, and this disproportionality increases through polyploidization.
Brodskiĭ
V Ia
VIa
Marshak
T L
TL
Avrushchenko
M Sh
MSh
Dungenova
R E
RE
D000818:Animals; D002466:Cell Nucleolus / Q000737:chemistry*; D003592:Cytophotometry / Q000295:instrumentation / Q000379:methods*; D008099:Liver / Q000737:chemistry / Q000648:ultrastructure; D008297:Male; D009687:Nuclear Proteins / Q000032:analysis*; D011689:Purkinje Cells / Q000737:chemistry / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D015203:Reproducibility of Results; D016622:Silver Staining
D009687:Nuclear Proteins
Vozmozhnost' tsitofotometrii iadryshkovykh belkov. Perspektivy issledovaniĭ sostoianiia iadryshkovogo apparata.