Direct evidence for degradation of esperamicin A1 with thiol confirmed by fast-atom bombardment mass spectrometry.
A degradation of esperamicin A1, which is a potent antitumor antibiotic having DNA-cleaving activity, was studied by fast-atom bombardment (FAB) mass spectrometry. It was proved that the degradation easily occurs via the thiol group(s) in the matrix solution and not by a FAB-induced reaction. It was concluded that from the FAB mass spectra of all non-volatile compounds, the molecular weight should be determined by using at least two chemically different matrices.
Takayama
M
M
Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Japan.
Nomura
T
T
Konishi
M
M
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D053281:Enediynes; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013438:Sulfhydryl Compounds
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D053281:Enediynes; D013438:Sulfhydryl Compounds; C047543:esperamicin A1