The effect of cis-diamminedichloroplatinum (II) on the formation of DNA-Pt-DNA cross-links.
The effect of cis-diamminedichloroplatinum (II) (cis-DDP) on the formation of interstrand cross-links in DNA and in DNA and chromatin complex from leukocytes was studied. Following the use of cis-DDP the number of DNA-DNA interstrand cross-links was elevated with the increase of cis-DDP concentration and elongation of reaction time. It was also found that nucleic proteins reduce the quantity of the cis-DDP induced DNA-DNA interstrand cross-links in the DNA in nucleoprotein complex when compared with the links in the isolated DNA.
Walter
Z
Z
Department of Molecular Genetics, University of Lódź, Poland.
Spychała
M
M
D000818:Animals; D002945:Cisplatin / Q000494:pharmacology*; D003432:Cross-Linking Reagents; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D007962:Leukocytes / Q000737:chemistry / Q000187:drug effects; D010984:Platinum / Q000378:metabolism*; D013552:Swine
D003432:Cross-Linking Reagents; D010984:Platinum; D004247:DNA; D002945:Cisplatin