Direct sequencing of double-stranded polymerase chain reaction-amplified 16S rDNA.
A number of different procedures have been developed for direct sequence analysis of PCR products. These methods rely on the cumbersome isolation of specific PCR products from agarose gels or the production of single-stranded template DNAs. In the approach presented here, we describe primers for the amplification of 16-S rDNA and a simple preparation of PCR product for sequencing.
Both
B
B
Institute for General Microbiology, University of Kiel, Federal Republic of Germany.
Krupp
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G
Stackebrandt
E
E
D001483:Base Sequence; D004275:DNA, Ribosomal / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D004277:DNA, Single-Stranded / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase
D004275:DNA, Ribosomal; D004277:DNA, Single-Stranded; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase