Endothelin-1-induced phosphorylation of the 20-kDa myosin light chain and caldesmon in porcine coronary artery smooth muscle.
Endothelin-1 (ET), a potent vasoconstrictor, induces a sustained increase in the phosphorylation level of the 20-kDa myosin light chain (MLC) in porcine coronary artery strips. ET also induces late phosphorylation of caldesmon, which is mimicked by 12-deoxyphorbol 13-isobutyrate, but not by 60 mM KCl. Nitroglycerin, a vasorelaxant, completely reverses the ET-induced phosphorylation of MLC, but not that of caldesmon. These results suggest an important regulatory role of MLC phosphorylation in ET-induced contraction.
Abe
Y
Y
Department of Internal Medicine, University of Tsukuba School of Medicine, Ibaraki, Japan.
Kasuya
Y
Y
Kudo
M
M
Yamashita
K
K
Goto
K
K
Masaki
T
T
Takuwa
Y
Y
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000378:metabolism*; D003331:Coronary Vessels / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D016232:Endothelins / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D009218:Myosins / Q000378:metabolism*; D005996:Nitroglycerin / Q000494:pharmacology; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D013552:Swine; D013997:Time Factors
D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D016232:Endothelins; D015240:Phorbol 12,13-Dibutyrate; D009218:Myosins; D005996:Nitroglycerin; D002118:Calcium