Effects of small-intestinal factors on hepatocyte development and modification of chemical agent-induced hepatopathy in rat.
We have clearly shown by primary culture of hepatocytes that a promoting factor for proliferation of parenchymal hepatocytes appeared in the portal blood of rats which received 70% partial hepatectomy, and that such a liver-regenerating factor exists not only in murine small-intestinal mucosa, but also in bovine intestinal mucosa at all times. We also observed that in hepatopathy induced in rats by chemical agents such as CCl4 and D-galactosamine, intraperitoneal injection of the bovine small-intestine mucosal extract containing this factor suppressed the increases of GOT, GPT and other enzyme activities in the rats' serum and modified the hepatopathy. These results indicate that the in-vitro effects of these factors on hepatocyte cultures reflect those found in vivo on hepatopathy.
Takahashi
N
N
Division of Agricultural Chemistry, School of Agriculture, Meiji University, Kanagawa, Japan.
Ohnaka
S
S
D000818:Animals; D001685:Biological Factors / Q000502:physiology*; D002251:Carbon Tetrachloride; D002452:Cell Count; D002478:Cells, Cultured; D056486:Chemical and Drug Induced Liver Injury / Q000503:physiopathology*; D004798:Enzymes / Q000097:blood; D005688:Galactosamine; D007413:Intestinal Mucosa / Q000737:chemistry; D007421:Intestine, Small / Q000737:chemistry*; D008099:Liver / Q000166:cytology*; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D001685:Biological Factors; D004798:Enzymes; D005688:Galactosamine; D002251:Carbon Tetrachloride