Direct derivative spectrophotometric determination of nitrazepam and clonazepam in biological fluids.
The use of fifth order derivative spectra allows the direct determination of nitrazepam in urine at 388 nm with a limit of detection of 1 microgram ml-1. The determination of nitrazepam in blood plasma can be carried out directly by measurement at 402 nm in the fourth order derivative spectra with a limit of detection of 1.5 micrograms ml-1. Clonazepam can be determined directly in urine samples at 384 nm by using sixth order derivative spectra with a limit of detection of 1 microgram ml-1 and in blood plasma by using fourth order derivative spectra at 384 nm with a limit of detection of 0.5 ppm.
Randez-Gil
F
F
Departamento de Química Analítica, University of Valencia, Burjassot, Spain.
Daros
J A
JA
Salvador
A
A
de la Guardia
M
M
D002998:Clonazepam / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D009567:Nitrazepam / Q000032:analysis* / Q000097:blood / Q000652:urine; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D002998:Clonazepam; D009567:Nitrazepam