High-performance liquid chromatographic determination of morphine, morphine-3-glucuronide, morphine-6-glucuronide and codeine in biological samples using multi-wavelength forward optical detection.
An isocratic high-performance liquid chromatographic method has been developed for the determination of morphine, morphine-3-glucuronide, morphine-6-glucuronide and codeine in plasma, urine and cerebrospinal fluid. The use of an efficient solid-phase extraction procedure together with a forward optical scanning detector allows a detection limit of 500 pg/ml. The method was evaluated by examination of biological samples taken from newborn infants following the intravenous administration of morphine sulfate.
Chari
G
G
Department of Pharmacodynamics, University of Illinois, Chicago 60612.
Gulati
A
A
Bhat
R
R
Tebbett
I R
IR
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003061:Codeine / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000652:urine*; D006801:Humans; D007231:Infant, Newborn; D007275:Injections, Intravenous; D009020:Morphine / Q000008:administration & dosage / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000652:urine*; D009022:Morphine Derivatives / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000652:urine*; D015203:Reproducibility of Results
D009022:Morphine Derivatives; C035349:morphine-6-glucuronide; D009020:Morphine; C018108:morphine-3-glucuronide; D003061:Codeine