Application of uniform design in L-isoleucine fermentation.
Uniform design was employed in our experiments for testing the effects of nine different compositions of the culture media for the production of L-isoleucine in Corynebacterium crenatum A11 fermentation. The most favorable medium was found to be composed of glucose 12%, (NH4)2SO4 2%, KH2PO4 0.1%, K2HPO4 0.45%, MgSO4.7H2O 0.055%, biotin 1 microgram, thiamine 20 micrograms, FeSO4.7H2O 2 ppm, MnSO4.4H2O 2 ppm, CaCO3 4% with pH 7.0-7.2. By using such medium, the amount of L-isoleucine produced by A11 was increased up to 15.1 mg/ml. Uniform design, as compared with orthogonal design, has the advantages of higher efficiency and easier analysis of the factors that affect the production.
Liu
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D
Laboratory of Microbiology, Shenyang College of Pharmacy, Liaoning, China.
Wang
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P
Li
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F
Li
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J
D000645:Ammonium Sulfate / Q000494:pharmacology; D001710:Biotin / Q000494:pharmacology; D002119:Calcium Carbonate / Q000494:pharmacology; D003352:Corynebacterium / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D005285:Fermentation; D005947:Glucose / Q000502:physiology; D007532:Isoleucine / Q000378:metabolism*; D008278:Magnesium Sulfate / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D017680:Potassium Compounds; D013831:Thiamine / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
D003470:Culture Media; D010710:Phosphates; D017680:Potassium Compounds; D007532:Isoleucine; D001710:Biotin; D008278:Magnesium Sulfate; D014508:Urea; C013216:potassium phosphate; D002119:Calcium Carbonate; D005947:Glucose; D011188:Potassium; D000645:Ammonium Sulfate; D013831:Thiamine