title: Simple high-performance liquid chromatographic method for the determination of a new phytochemical drug, fellavine, and its metabolites in human and rat plasma and urine.
abstract: The use of a small precolumn instead of an injection loop for the determination of a new phytochemical drug, fellavine, and its metabolites is described. The method combines the direct injection of plasma and urine into the reversed-phase precolumn with separation on a Spheri-5 RP-18 analytical column. Different sorbents in the precolumn were compared. A recovery of fellavine and its metabolites from biological fluids except rat plasma of almost 100% was achieved on Chrompack RP (30-40 microns) and LiChrosorb RP-18 (7 microns). For rat plasma only the last sorbent gave 80% fellavine recovery. The influence of the protein binding on the fellavine recovery was examined. The limit of detection was equal to 0.05 micrograms/ml fellavine for plasma and 0.02 micrograms/ml for urine. To enhance the limit of detection longer precolumns were perferred.
authors.affiliation: Department of Organic Chemistry, Faculty of Pharmaceutical Science, Moscow Medical Academy, USSR.
authors.forename: N A
authors.initials: NA
authors.lastname: Tjukavkina
authors.forename: S V
authors.initials: SV
authors.lastname: Geodakyan
authors.forename: A I
authors.initials: AI
authors.lastname: Khvostova
authors.forename: J A
authors.initials: JA
authors.lastname: Kolesnik
authors.forename: V I
authors.initials: VI
authors.lastname: Glyzin
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000097:blood* / Q000652:urine*; D006801:Humans; D051381:Rats
chemical_list: D005419:Flavonoids; C016043:phellamurin