Immunocytochemical demonstration of DSIP-like immunoreactivity in the hypothalamus of the rat.
The distribution of delta sleep-inducing peptide immunoreactivity (DSIP-IR) was studied in the rat diencephalon. Varicose nerve fibers exhibiting DSIP-IR were found throughout the mediobasal hypothalamus, most frequently in the hypothalamic arcuate nucleus and in the adjoining median eminence and pituitary stalk. This innervation provides a basis for the involvement of DSIP in neuroendocrine regulation at the hypothalamic level. In the hypothalamus, DSIP-IR innervation was also observed close to the third ventricle and within the mamillary complex. Despite pretreatment with colchicine, no evidence of immunoreactive cell bodies containing DSIP-IR could be found.
Skagerberg
G
G
Department of Medical Cell Research, University of Lund, Sweden.
Bjartell
A
A
Vallet
P G
PG
Charnay
Y
Y
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000166:cytology; D003701:Delta Sleep-Inducing Peptide / Q000032:analysis* / Q000276:immunology; D007031:Hypothalamus / Q000033:anatomy & histology / Q000166:cytology*; D007150:Immunohistochemistry; D008297:Male; D008473:Median Eminence / Q000166:cytology; D009412:Nerve Fibers / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
D003701:Delta Sleep-Inducing Peptide