Effect of refraction on optical microscopic measurement of internal blood-vessel diameter and its correction.
In optical microscopic measurement of internal blood-vessel diameters, the effect of refraction must be taken into account to ensure accuracy of the result. This effect is discussed and an analytical correction formula derived. Phantom blood vessels with known internal and external diameters were used to test the validity of the correction formula. The errors obtained prior to correction were reduced significantly after correction.
Chen
E Q
EQ
Department of Biometry, Medical University of South Carolina, Charleston 29425.
Lam
C F
CF
Periasamy
A
A
D001808:Blood Vessels / Q000033:anatomy & histology*; D006801:Humans; D008433:Mathematics; D008853:Microscopy / Q000379:methods*; D012031:Refractometry