[Exogenous NADPH restores the detoxication function of isolated hepatocytes lost in the process of freezing-thawing].
Freezing and thawing have been studied for their effect on the rat hepatocyte detoxication system. Freezing-thawing of hepatocytes in the medium without cryoprotectors is to impair functioning of the biotransformation system of biphenyl, a hydrophobic xenobiotic. It is found that inhibition of the first stage of xenobiotics biotransformation is a result of the loss of pyridine nucleotides and substrates necessary for NADP+ reduction.
Sukach
A N
AN
Petrenko
A Iu
AIu
D000818:Animals; D001713:Biphenyl Compounds / Q000493:pharmacokinetics*; D005615:Freezing; D066298:In Vitro Techniques; D008658:Inactivation, Metabolic / Q000502:physiology; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D009249:NADP / Q000502:physiology*; D009711:Nucleotides / Q000378:metabolism; D051381:Rats; D015262:Xenobiotics / Q000493:pharmacokinetics
D001713:Biphenyl Compounds; D009249:NADP; D009711:Nucleotides; D015262:Xenobiotics; C010574:biphenyl
Ekzogennyĭ NADPH vosstanavlivaet detoksikatsionnuiu funktsiiu izolirovannykh gepatotsitov, utrachennuiu v protsesse zamorazhivaniia-otogreva.