New rapid method for the study of Pneumocystis carinii interaction with alveolar macrophages.
Understanding the pathophysiology of Pneumocystis carinii infection has been limited by the availability of methods for precisely measuring the interaction of P. carinii with host cells. Here we describe a new method which allows for the rapid assessment of P. carinii binding to, and internalization by, adherent alveolar macrophages. The method is based on the detection of fluorescein-labelled P. carinii by an automated fluorescence measurement system.
Koziel
H
H
MacArthur Center for Molecular Parasitology, Yale University, New Haven, CT.
Williams
D J
DJ
Armstrong
M Y
MY
Richards
F F
FF
Fishman
J A
JA
Ezekowitz
R A
RA
Warner
A
A
Fuglestad
J
J
Rose
R M
RM
D002478:Cells, Cultured; D016650:Fluorescein-5-isothiocyanate; D005470:Fluorometry; D006801:Humans; D016676:Macrophages, Alveolar / Q000382:microbiology*; D011010:Pneumocystis / Q000276:immunology*
D016650:Fluorescein-5-isothiocyanate