A matrix-assisted laser desorption time-of-flight mass spectrometer based on a 600 ps, 1.2 mJ nitrogen laser.
The design and performance of a short-pulse UV laser desorption time-of-flight mass spectrometer is described. The instrument combines the advantages of a 600 ps, 337 nm pulsed nitrogen laser with a compact, high-voltage extraction linear time-of-flight analyzer. A number of peptides and proteins have been analyzed to demonstrate sensitivity, high mass range, resolution and mixture analysis.
Chevrier
M R
MR
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Cotter
R J
RJ
D000595:Amino Acid Sequence; D004867:Equipment Design; D006801:Humans; D066298:In Vitro Techniques; D007834:Lasers; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods; D009074:Melanocyte-Stimulating Hormones / Q000737:chemistry; D008836:Micrococcal Nuclease / Q000737:chemistry; D008969:Molecular Sequence Data; D009113:Muramidase / Q000737:chemistry
D009074:Melanocyte-Stimulating Hormones; D008836:Micrococcal Nuclease; D009113:Muramidase