A new family of sugar-inducible expression vectors for Escherichia coli.
A set of 11 expression vectors was constructed, each of them harbouring a cloning cassette under the control of the araB promoter. Some of these vectors enable expression of foreign proteins in the cytoplasm, while others include a synthetic sequence coding for a very efficient secretion signal sequence. Other features are an f1 origin of replication (in plus or minus orientation) and a promoter(up) mutation that enhances the already very high level of expression from these vectors. With such a versatile vector family, cloning, sequencing and site-directed mutagenesis can be performed on the same vector, and the level of expression can be defined according to the specific constraints of a given protein.
Cagnon
C
C
INSA, URA 544 du CNRS, Centre de transfert en Biotechnologie-Microbiologie, Toulouse, France.
Valverde
V
V
Masson
J M
JM
D000595:Amino Acid Sequence; D001483:Base Sequence; D004790:Enzyme Induction / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D001616:beta-Galactosidase / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics
D011994:Recombinant Proteins; D001616:beta-Galactosidase; D001618:beta-Lactamases