Vital reactions in rat skin wounds by quantitation of fibrin.
The fibrin contents in contused skin wounds that were experimentally inflicted on rats were measured by a new method which was based on the determination of solubilized fibrin degradation products (FDP) using a latex agglutination test after plasmin digestion of tissue homogenates. The levels of FDP in ante-mortem wounds began to increase within 10 min after wounding and continued to increase for up to 6 h. The FDP levels in post-mortem wounds remained under the detection limit of the method used and were identical to those of intact tissues. The fibrin contents in the ante-mortem wounds prepared 2 days after death did not differ significantly from those prepared immediately. The results suggest that this method may be applicable to the identification of the vital reaction of the wound.
Fujitani
N
N
Department of Forensic Medicine, Saga Medical School, Japan.
Matoba
R
R
Inoue
H
H
Takabe
F
F
D000818:Animals; D005337:Fibrin / Q000032:analysis*; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D005455:Fluorescent Antibody Technique; D005554:Forensic Medicine; D007841:Latex Fixation Tests; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000293:injuries* / Q000378:metabolism
D005338:Fibrin Fibrinogen Degradation Products; D005337:Fibrin