title: Comparison of the effects of semi-occlusive polyurethane dressings and hydrocolloid dressings on dermal repair: 1. Cellular changes.
abstract: The effects on dermal repair of two wound dressings, one the semi-occlusive polyurethane sheet Opsite, the other the hydrocolloid Granuflex, were compared in full-thickness excised lesions on porcine skin during the period from 5 d to 6 months after injury. Quantitative studies were made of changes in the populations of polymorphonuclear leucocytes, macrophages, fibroblasts, and endothelial cells. The progress of repair in the wounds covered with the semi-occlusive dressing showed a decrease in the number of inflammatory cells (polymorphonuclear leukocytes and macrophages) from 5 to 60 d, whereas the number of proliferative phase cells (fibroblasts and endothelial cells) increased from 5 to 7 d. The total cellularity per unit area showed an increase between 5 and 7 d, that is, during the proliferative phase of repair, and then progressively decreased as the proliferative phase was succeeded by the remodeling phase. In contrast, the repair process in the hydrocolloid-dressed wounds was more complex. The number of inflammatory cells remained relatively high throughout and there were consistently fewer endothelial cells present throughout. Fibroblast number showed an initial fall from 5 to 14 d but then started to increase in number from 21 to 60 d. This chronic inflammatory reaction appeared to be in response to particulate matter that had been incorporated into the wound bed and hypodermis, and was still apparent 6 months after injury, when hydrocolloid particles were detectable microscopically in the hypodermis.
authors.affiliation: Division of Anatomy and Cell Biology, United Medical School of Guy's Hospital, London, U.K.
authors.forename: S R
authors.initials: SR
authors.lastname: Young
authors.forename: M
authors.initials: M
authors.lastname: Dyson
authors.forename: R
authors.initials: R
authors.lastname: Hickman
authors.forename: S
authors.initials: S
authors.lastname: Lang
authors.forename: C
authors.initials: C
authors.lastname: Osborn
mesh_terms: D000818:Animals; D003102:Colloids / Q000494:pharmacology*; D009779:Occlusive Dressings; D010784:Photomicrography / Q000379:methods; D011140:Polyurethanes / Q000494:pharmacology*; D013552:Swine; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D003102:Colloids; D011140:Polyurethanes