title: Partial amino acid sequence of the light chain of human anti-Rh(D) monoclonal antibody H2D5D2F5.
abstract: Antibody H2D5D2F5 is a human monoclonal anti-Rh(D) IgG1 (lambda) produced by Epstein-Barr virus-immortalized B lymphocytes from a healthy donor. The complete amino acid sequence of the light (L) chain, with the exception of positions 94-97, was determined by Edman degradation of the intact chain, containing 30 residues, and derived tryptic and thermolytic peptides. Sequences of the peptides were aligned by comparison with the sequences of previously reported L chains. H2D5D2F5 L chain belongs to the first variable subgroup of human chains. Its sequence does not reveal striking differences when compared to those of other human lambda chains issued from myeloma or hybridoma.
authors.affiliation: Unité de Chimie des Proteines, Institut Pasteur, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Dlouha
authors.forename: A
authors.initials: A
authors.lastname: Lecroisey
authors.forename: A
authors.initials: A
authors.lastname: Henschen
authors.forename: Y
authors.initials: Y
authors.lastname: Ruttyn
authors.forename: P
authors.initials: P
authors.lastname: Rouger
authors.forename: B
authors.initials: B
authors.lastname: Keil
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000737:chemistry*; D008969:Molecular Sequence Data; D012204:Rh-Hr Blood-Group System / Q000276:immunology*; D016415:Sequence Alignment; D014357:Trypsin
chemical_list: D000596:Amino Acids; D000911:Antibodies, Monoclonal; D007147:Immunoglobulin Light Chains; D012204:Rh-Hr Blood-Group System; D014357:Trypsin