Simple method for the determination of morphine and its active glucuronide metabolite in human plasma by high-performance liquid chromatography with electrochemical detection.
A simple method for the simultaneous determination of morphine and its pharmacologically active metabolite morphine-6-glucuronide in 0.5 ml human plasma is described. It is based on the method of Svensson [J. Chromatogr., 230 (1982) 427 and 375 (1986) 174], but uses only one solid-phase extraction cartridge prior to chromatography and only a 20-microliter injection volume. Mean recoveries of 90 and 85% for morphine and morphine-6-glucuronide, respectively, were obtained, the limit of detection being 2 nmol/l (at a signal-to-noise ratio of 3.0).
Mason
J L
JL
University Department of Anaesthesia, Queens Medical Centre, Nottingham, UK.
Ashmore
S P
SP
Aitkenhead
A R
AR
D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D009020:Morphine / Q000097:blood*; D009022:Morphine Derivatives / Q000097:blood*; D015203:Reproducibility of Results
D009022:Morphine Derivatives; C035349:morphine-6-glucuronide; D009020:Morphine