Peripheral blood mononuclear cells in the squirrel monkey Saimiri sciureus. II. Exploration of the IL2/IL2R pathway of T-cell activation.
We report here on functional aspects of squirrel monkey T cells isolated either from peripheral blood mononuclear cells or in the presence of accessory cells or accessory-cell-secreted cytokines. The T-cell response triggered by polyclonal activators was measured through proliferation, T-cell growth factor production and transient expression of receptors to growth factors. We also sought to delineate mechanisms by which antimitogenic molecules might prevent lymphocytes from being able to express "activation antigens" and/or from proliferating. Under these conditions, we mainly explored the inducible IL2/IL2R pathway and phenomena occurring in lymphocyte "non-activation" in terms of early and late cellular events; indeed, defects in the IL2/IL2R pathway represent one of the dysregulation events presumed to occur during the acute phase of human malaria, disease for which. Saimiri monkeys have been selected as experimental hosts.
Garraud
O
O
Laboratoire d'Immunologie parasitaire, Institut Pasteur de la Guyane Française, Cayenne.
Perraut
R
R
Gysin
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D007376:Interleukin-2 / Q000096:biosynthesis / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D008288:Malaria / Q000276:immunology; D008297:Male; D008934:Mitogens / Q000276:immunology; D010835:Phytohemagglutinins; D011972:Receptor, Insulin / Q000096:biosynthesis; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis / Q000276:immunology*; D011990:Receptors, Transferrin / Q000096:biosynthesis; D012453:Saimiri / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D008934:Mitogens; D010835:Phytohemagglutinins; D015375:Receptors, Interleukin-2; D011990:Receptors, Transferrin; D011972:Receptor, Insulin