Role of exogenous ascorbic acid in tissue status of ascorbic acid-2-sulphate in guinea pigs.
Guinea pigs were given ascorbic acid orally in two doses; a low and a high dose. The tissue levels of ascorbic acid-2-sulphate was estimated in these animals after 15 days of feeding and a subsequent deprivation period of 15 days. The specific activity of the enzymes ascorbic acid sulphotransferase and ascorbic acid-2-sulphate sulphohydrolase was studied. During higher ascorbic acid intake, the activity of ascorbic acid sulphotransferase was increased, whereas ascorbic acid-2-sulphate sulphohydrolase showed a decreased activity. But when ascorbic acid intake was lowered or ceased, the activity of the above enzymes showed a reverse pattern. Possible reasons for the lack of antiscorbutic activity of ascorbic acid-2-sulphate in guinea pigs is discussed.
Pillai
G R
GR
Department of Biochemistry, University of Kerala, Trivandrum, India.
Indira
M
M
Vijayammal
P L
PL
D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D006168:Guinea Pigs; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D009928:Organ Specificity
C001956:ascorbic acid 2-sulfate; D001205:Ascorbic Acid