Zeta potential of human X- and Y-bearing sperm.
The zeta potential of human X- and Y-bearing sperm was measured by two different methods: (i) using an electrophoretic light scattering spectrophotometer, and (ii) using a laser-rotating prism. The X- and Y-bearing sperm were separated by free-flow electrophoresis, and their purities were determined by staining for the F-body using quinacrine mustard. The zeta potential of the sperm in the fraction containing more than 80% Y-bearing sperm was approximately -16 mV, whereas that of sperm in the fraction containing more than 95% X-bearing sperm was approximately -20 mV. In other words, the net negative-charge on the cell surface of human X-bearing sperm is higher than that of Y-bearing sperm.
Ishijima
S A
SA
Department of Biology, College of Arts and Sciences, University of Tokyo, Japan.
Okuno
M
M
Mohri
H
H
D002469:Cell Separation; D004594:Electrophysiology; D006801:Humans; D008027:Light; D008297:Male; D012542:Scattering, Radiation; D013053:Spectrophotometry; D013094:Spermatozoa / Q000502:physiology* / Q000648:ultrastructure; D014960:X Chromosome; D014998:Y Chromosome