title: Heat aggregation studies of phycobilisomes, ferritin, insulin, and immunoglobulin by dynamic light scattering.
abstract: Dynamic laser light scattering studies on the heat aggregation behavior of phycobilisomes (PBS), ferritin, insulin, and immunoglobulin (IgG) in dilute aqueous solutions has been reported. Except for PBS, results are reported for heat aggregation trends in these proteins for three different pH environments (4.0, 7.5, 9.1). For PBS, studies were performed only in the neutral buffer medium (pH 7.5). The experiments were performed in the very dilute concentration regime (between 0.23 and 1.8 gL-1). For all these samples heat aggregation and dissociation trends were found to be linear with temperature. Upon temperature reversal (self-cooling), hysteresis-like behavior observed in insulin was found to be predominantly large at pH 7.5. PBS, ferritin, and IgG showed no such behavior at any of three pH values, and retraced their path of aggregation while dissociating on temperature reversal. Heat aggregation and dissociation processes in ferritin were found to be independent of pH. The IgG samples showed smooth aggregation tendency only up to 35 degrees C in the buffer media pH 4.0 and 9.1, whereas for pH 7.0 the same could be observed until 60 degrees C. Low polydispersity in the correlation spectra was observed in case of all these samples.
authors.affiliation: Department of Physics, Indian Institute of Technology, New Delhi.
authors.forename: B P
authors.initials: BP
authors.lastname: Singh
authors.forename: H B
authors.initials: HB
authors.lastname: Bohidar
authors.forename: S
authors.initials: S
authors.lastname: Chopra
mesh_terms: D005293:Ferritins / Q000737:chemistry*; D006358:Hot Temperature; D007074:Immunoglobulin G / Q000737:chemistry*; D007328:Insulin / Q000737:chemistry*; D008027:Light; D045342:Light-Harvesting Protein Complexes; D045524:Phycobilisomes; D010860:Pigments, Biological / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry*; D012542:Scattering, Radiation; D013816:Thermodynamics
chemical_list: D007074:Immunoglobulin G; D007328:Insulin; D045342:Light-Harvesting Protein Complexes; D045524:Phycobilisomes; D010860:Pigments, Biological; D010940:Plant Proteins; D005293:Ferritins