title: In vivo utilization of N-(phosphonomethyl)-anilines and related substances by Pseudomonas spec. GS.
abstract: Utilization of various phosphonates as source for phosphorus by the glyphosate degrading strain Pseudomonas spec. GS was investigated. Metabolites of phosphonate degradation were characterized indicating the cleavage of the C-P-bound as primary step of breakdown. The phosphonate N-(phosphonomethyl)-4'-nitroazobenzene-4-amine (azophon) was characterized as a suitable substrate for detection of C-P-bond splitting activity in vivo. Pseudomonas cells permeabilized by toluene treatment were also capable of phosphonate degradation whereas no in vitro activity of a putative C-P-bond cleaving enzyme was detectable after cell disruption.
authors.affiliation: Institut für Biochemie der Pflanzen Halle, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Albrecht
authors.forename: R
authors.initials: R
authors.lastname: Weidhase
authors.forename: M
authors.initials: M
authors.lastname: Stock
authors.forename: R A
authors.initials: RA
authors.lastname: Weidhase
mesh_terms: D000814:Aniline Compounds / Q000378:metabolism*; D002455:Cell Division; D005998:Glycine / Q000031:analogs & derivatives* / Q000378:metabolism; D006540:Herbicides / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*; D000097797:Glyphosate
chemical_list: D000814:Aniline Compounds; D005998:Glycine; D006540:Herbicides; D009943:Organophosphorus Compounds; D000097797:Glyphosate; C032627:methylphosphonic acid; C023650:aniline