title: Use of an ophthalmologic ultrasoundscanner in human onchocercal skin nodules for non-invasive sequential assessment during a macrofilaricidal trial with amocarzine in Guatemala. The first experiences.
abstract: Ultrasonography of onchocercal skin nodules was performed with an ophthalmologic real time linear scanner with a B probe of 10 MHz. A clinical trial in Guatemala with amocarzine (CGP 6140)--a new oral macrofilaricidal compound--investigated three repeat dose regimens and one placebo control group, each group consisting of six patients. Onchocercal nodules were scanned before treatment and on day 10, 30 and 60 after start of amocarzine. A total of 28 treated and 8 additional untreated nodules were analysed and compared with the histologic findings following nodulectomy at day 60. Of the 28 treated nodules, 21 were of onchocercal origin and seven were lymph nodes. The correlation between ultrasonography and histology was good in 25 patients, but did not match in three. In 20 out of 21 treated nodules a progressive ultrasonographic change over two months was seen. Of the eight additional untreated nodules, five were of onchocercal origin, one was a lymph node, one an epidermoid cyst and in one only fibrous tissue was detected. The ultrasonography correlated well to histology in seven nodules but not in one. In five onchocercal nodules no change was observed over two months. For initial control purposes six nodules were excised around day 10, four were of onchocercal origin and two were lymph nodes. The correlation was good in four. The present results indicate that an ophthalmologic real time linear scanner can be used in the bidimensional mode as a non-invasive method to assess sequentially the events in superficial onchocercal nodules following chemotherapy with amocarzine. This is the first objective non-invasive method permitting sequential assessment of the content of onchocercal nodules and it is far superior than subjective sequential manual palpation.
authors.affiliation: CIBA-GEIGY Limited, Basle, Switzerland.
authors.forename: A A
authors.initials: AA
authors.lastname: Poltera
authors.forename: O
authors.initials: O
authors.lastname: Reyna
authors.forename: G
authors.initials: G
authors.lastname: Zea-Flores
authors.forename: F
authors.initials: F
authors.lastname: Beltranena
authors.forename: A
authors.initials: A
authors.lastname: Nowell de Arevalo
authors.forename: F
authors.initials: F
authors.lastname: Zak
mesh_terms: D000818:Animals; D005369:Filaricides / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D005500:Follow-Up Studies; D006166:Guatemala; D006801:Humans; D008198:Lymph Nodes / Q000000981:diagnostic imaging; D008297:Male; D009854:Onchocerca / Q000187:drug effects* / Q000302:isolation & purification; D009855:Onchocerciasis / Q000000981:diagnostic imaging* / Q000188:drug therapy / Q000469:parasitology; D010879:Piperazines / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D012867:Skin / Q000000981:diagnostic imaging* / Q000469:parasitology; D014463:Ultrasonography
chemical_list: D005369:Filaricides; D010879:Piperazines; C051744:amocarzine