title: Application of an enzyme immunoassay for detecting antibodies in sera of Macaca fascicularis naturally exposed to Mycobacterium tuberculosis.
abstract: An enzyme immunoassay (EIA) was developed for detecting mycobacterial antibodies in the sera of 22 Macaca fascicularis following a natural outbreak of tuberculosis. EIAs were conducted using four antigens (lysozyme, triton, or deoxycholate extracts of Mycobacterium tuberculosis or a purified protein derivative) and two conjugates (protein A or antihuman). Mycobacterial antibodies were detected in two of two culture-positive monkeys, in nine of ten tuberculin test-suspect monkeys (culture-negative), and in five of ten tuberculin test-negative monkeys (culture-negative). Results indicate EIA may be of practical value in detecting monkeys exposed to M. tuberculosis.
authors.affiliation: United States Army Medical Research Institute of Chemical Defense, Veterinary Medicine and Laboratory Resources Division, Aberdeen Proving Ground, MD 21010-5425.
authors.forename: K D
authors.initials: KD
authors.lastname: Corcoran
authors.forename: C O
authors.initials: CO
authors.lastname: Thoen
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D007124:Immunoenzyme Techniques; D008252:Macaca fascicularis; D008992:Monkey Diseases / Q000175:diagnosis*; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D014374:Tuberculin Test / Q000662:veterinary; D014376:Tuberculosis / Q000175:diagnosis / Q000662:veterinary*
chemical_list: D000907:Antibodies, Bacterial