[Proliferation of smooth muscle cells in hypertension].
The aortic intima and media isolated from hypertensives showed a significantly larger number (up to 20%) of smooth muscle cells (SMC) with tetraploid DNA content. The similar process was shown to be also a part of normal human vessel maturation. Normotensive human and rat aortic SMCs were found to accumulate 3H-thymidine, have a lower proliferative ability and they were apt to polyploidize in the primary culture. Such a population could not be detected in the aorta of spontaneously hypertensive rats. It was ascertained that 10 microM of noradrenaline significantly increased (approximately by 2 times) the occurrence of true polyploid cells in the rat aortic SMC subculture. The effect of noradrenaline was blocked by the concomitant effects of alpha- and beta-adrenoreceptor antagonists. SMC polyploidization was also stimulated by the simultaneously use of the direct activators of second messenger systems forskolin and phorbol-12-myristate-13-acetate. Thus, the SMC subpopulation that is apt to polyploidize exists in normal vessels and noradrenaline may be one of the mediators of a response of the vascular wall SMC, which seems to occur due to the synergism of two second messenger systems.
Printseva
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Tiurmin
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D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000473:pathology*; D002452:Cell Count / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D003470:Culture Media; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D006973:Hypertension / Q000473:pathology*; D066298:In Vitro Techniques; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000473:pathology*; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D011918:Rats, Inbred SHR; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical
D003470:Culture Media; D009638:Norepinephrine
Proliferatsiia gladkomyshechnykh kletok pri gipertonii.