title: [Clinical significance of human basophil releasability in bronchial asthma].
abstract: Human basophil releasability (HBR) was determined in 50 patients with asthma by modified human basophil degranulation test (HBDT). The result of HBDT was expressed as degranulation index (DI). 1. Anti-IgE-induced HBR: the mean value of DI in extrinsic asthma, intrinsic asthma and control subjects was 50.1 +/- 2.26(%), 32.2 +/- 2.3(%) and 18 +/- 2.74(%) (x +/- sem) respectively. If a DI of 30% or greater was considered as positive, the positive rate in the above three groups would be 96.9%, 60.0% and 20.0% respectively. There was significant difference between any two of the three groups (P less than 0.01). 2. Allergen-induced HBR: HBDT was performed in the asthmatics with sixteen allergens and in the controls with mite allergen. The positive rates were 68% in the patients and 0% in the controls. The coincident rate of the results between HBDT and skin test reached 82.9%. The results showed that anti-IgE-induced HBR could be regarded as an objective parameter for differentiating extrinsic and intrinsic asthma and allergen-induced HBR could be considered as an useful means for in vitro allergen diagnosis of asthma.
authors.affiliation: Department of Medicine, Second Affiliated Hospital, Zhejiang Medical University, Hangzhou.
authors.forename: H H
authors.initials: HH
authors.lastname: Shen
authors.forename: F G
authors.initials: FG
authors.lastname: Liu
authors.forename: Y M
authors.initials: YM
authors.lastname: Hu
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000008:administration & dosage; D001249:Asthma / Q000145:classification / Q000175:diagnosis*; D015549:Basophil Degranulation Test; D002648:Child; D002675:Child, Preschool; D006801:Humans; D008875:Middle Aged
chemical_list: D000485:Allergens