Locomotor bias produced by intra-accumbens and intracaudate injection of polyclonal dopamine anti-idiotypic antibodies.
The preceding article described anti-idiotypic antibodies to conjugated dopamine (AIDA); results were consistent with the hypothesis that these antibodies contained the internal image of conjugated dopamine (DA-G-BSA) and binded to dopamine (DA) receptors. We further tested these anti-idiotypic antibodies to conjugated dopamine by examining the functional changes produced by unilateral injection of AIDA (or DA-G-BSA) into the nucleus accumbens or into the medio-dorsal caudate in mice. Our results showed that unilateral injection of AIDA (or DA-G-BSA) into the nucleus accumbens or into the medio-dorsal caudate produced an ipsilateral locomotor asymmetry in amphetamine-treated animals which was similar to the one produced by unilateral intra-caudate injection of haloperidol (a non-specific DA antagonist). The asymmetry was site specific: injection of AIDA around the nucleus accumbens or into the anterior caudate was not effective. The asymmetry was also shown not to depend on the peripheral injection of amphetamine. Taken together, the present results and those in the preceding article suggest that the AIDA contains the internal image of DA-G-BSA and that these two substances bind to DA receptors to produce behavioral changes.
Mrabet
O
O
Laboratoire de Neurosciences Comportementales et Cognitives, URA CNRS n. 339, Université de Bourdeaux I, Talence, France.
Messier
C
C
Mons
N
N
Destrade
C
C
Geffard
M
M
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D002421:Caudate Nucleus / Q000187:drug effects / Q000502:physiology*; D003913:Dextroamphetamine / Q000494:pharmacology; D004298:Dopamine / Q000276:immunology / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D006220:Haloperidol / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009043:Motor Activity / Q000187:drug effects; D011954:Receptors, Dopamine / Q000502:physiology
D000888:Antibodies, Anti-Idiotypic; D011954:Receptors, Dopamine; D006220:Haloperidol; D003913:Dextroamphetamine; D004298:Dopamine