Determination of nitecapone and (13C6)nitecapone in human plasma by gas chromatography/mass spectrometry.
A gas chromatographic/mass spectrometric method is developed and validated for simultaneous determination of nitecapone and its 13C6-labelled analogue in human plasma using (2H6,13C6)nitecapone as internal standard. The method involves extraction of the analytes from plasma to ethyl acetate-hexane mixture (20:80) and conversion to bis(trimethylsilyl) ethers prior to determination by gas chromatography/mass spectrometry using selected ion monitoring. The quantification range is 0.5-2000 ng ml-1. Precision ranges from 11.3% (coefficient of variation) at low levels to 2.4% at high levels. Recovery is about 50% in the whole range. The method is applied to a pharmacokinetic study where nitecapone diluted with 14C-labelled nitecapone is given intravenously concomitantly with an oral dose of (13C6)nitecapone.
Ottoila
P
P
Orion Pharmaceutica, Espoo, Finland.
Pakkala
E
E
Karlsson
C
C
Taskinen
J
J
D002247:Carbon Isotopes; D065098:Catechol O-Methyltransferase Inhibitors; D002396:Catechols / Q000097:blood* / Q000493:pharmacokinetics; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008297:Male; D010422:Pentanones / Q000097:blood* / Q000493:pharmacokinetics
D002247:Carbon Isotopes; D065098:Catechol O-Methyltransferase Inhibitors; D002396:Catechols; D010422:Pentanones; C057364:nitecapone