Molecular diversity of erythrocyte calpastatin.
According to the difference of molecular masses estimated by SDS-polyacrylamide gel electrophoresis, calpastatins are classified into two types, i.e. muscle type (110 kDa) and erythrocyte type (70 kDa). Muscle type calpastatin contains four internally repetitive sequences (Domains 1-4) and one nonhomologous sequence on the amino-terminal side (Domain L), whereas erythrocyte type lacks Domains L and 1. By immuno-blot analysis, chicken erythrocytes, nucleated cells, were found to contain muscle type calpastatin. In avian erythrocytes, diminution of the calpastatin molecule as in mammalian erythrocytes was not observed.
Takano
E
E
Department of Clinical Science and Laboratory Medicine, Faculty of Medicine, Kyoto University, Japan.
Ueda
M
M
Tsunekawa
S
S
Murakami
T
T
Maki
M
M
Hatanaka
M
M
Murachi
T
T
D000818:Animals; D002135:Calcium-Binding Proteins / Q000097:blood* / Q000145:classification / Q000302:isolation & purification; D002154:Calpain / Q000037:antagonists & inhibitors; D002645:Chickens; D004912:Erythrocytes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008970:Molecular Weight; D014018:Tissue Distribution
D002135:Calcium-Binding Proteins; C033668:calpastatin; D002154:Calpain