[Immunochemical determination of human thiol protein disulfide oxidoreductase in cell and tissue homogenates by competitive EIA].
Different monospecific antisera against thiol-protein disulfide oxidoreductase (TPO, EC 1.8.4.2, protein-disulfide isomerase, EC 5.3.4.1) were raised in rabbits by immunization with purified human TPO and characterized by means of Laurell and immunoblot techniques. A competitive anti-TPO-EIA with insolubilized TPO has been used to determine this enzyme in cells and tissue homogenates. The assay shows a sensitivity of 1.2 ng/ml and a specificity of about 99%. The TPO content in relation to the total protein was found to be: in pancreas 0.65%, liver 0.45%, spleen 0.12%, placenta 0.16%, tonsils 0.06% and lymph nodes 0.03%.
Kröning
H
H
Forschungsabteilung Experimentelle Immunologie, Medizinischen Akademie Magdeburg.
Mansfeld
H W
HW
Held
C
C
Thiel
U
U
Ansorge
S
S
D015153:Blotting, Western; D004220:Disulfides / Q000378:metabolism; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007124:Immunoenzyme Techniques; D007535:Isomerases / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D010088:Oxidoreductases / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology; D019704:Protein Disulfide-Isomerases
D004220:Disulfides; D010088:Oxidoreductases; D007535:Isomerases; D019704:Protein Disulfide-Isomerases
Immunochemische Bestimmung der humanen Thiol-Proteindisulfid-Oxidoreduktase in Zell- und Gewebshomogenaten mit Hilfe eines kompetitiven EIA.