[Sialyltransferase activity in the spermatic membrane and its relation to human fertility and sterility].
Sialyltransferase activity was determined on normospermic men sperm cells (greater than 80 x 10(6) sperm/ml and 75% motility) and oligospermic infertile sperm cells (less than 20 x 10(6) sperm/ml and less than 20% motility) and asthenospermic (greater than 40 x 10(6) sperm/ml and less than 10% motility). Sialytransferase activity is quantified by means of the transference of radioactivity of CMP-3H-sialic acid toward the exogenous acceptor (asialofetuin). The enzyme substrate complexes formed in presence of phosphotungstic acid result precipitated insoluble, which was retained on glass fiber filter. The sialyltransferase activity decrease in oligospermic sperm cells 62 +/- 3% and in the asthenospermic decreased 57 +/- 4% with respect normospermic sperm cells. The decrement on sialyltransferase activity in the infertile sperm, permits to assume that this enzyme probably participates as a direct cause of its pathology with detrimental structural and functional integrity of the plasma membrane.
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Unidad de Investigación Clínica, Hospital de Gineco-Obstetricia Luis Castelazo Ayala, México, D.F.
Salazar
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D002462:Cell Membrane / Q000201:enzymology; D004796:Clinical Enzyme Tests / Q000379:methods*; D005298:Fertility / Q000502:physiology*; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis*; D008297:Male; D009845:Oligospermia / Q000175:diagnosis; D012016:Reference Values; D012799:Sialyltransferases / Q000032:analysis*; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000201:enzymology*
D012799:Sialyltransferases
Actividad de sialiltransferasa en la membrana espermática y su relación con fertilidad y esterilidad humanas.