[A method of determining the levels of deoxynivalenol (vomitoxin) and its metabolites].
A method has been developed for assay of deoxynivalenol (DON) and its metabolite desepoxideoxynivalenol (DOM-1) in animal excrements with the use of reversed-phase high-performance liquid chromatography with UV-detection (218 nm). The method includes purification in the mini-column with activated carbon and aluminium oxide. The detection limit was 50 ng/g, relative standard deviation--0.05-0.1, the degree of toxin isolation--76-89%. DON isolated from Fusarium macroceras, strain 579a, cultivated in rice under laboratory conditions, and DOM-1 obtained as a result of DON incubation with the contents of the beef first stomach, were used in the study. The structure of toxins isolated has been proved by the mass-spectrometry method. The method developed by the authors was used in the study of DON metabolism in vivo in monkeys.
Tutel'ian
V A
VA
Sobolev
V S
VS
Katrush
I R
IR
Medvedev
F A
FA
Zakharova
L P
LP
D000818:Animals; D002521:Cercopithecus; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005243:Feces / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D051381:Rats; D014255:Trichothecenes / Q000302:isolation & purification* / Q000652:urine
D014255:Trichothecenes; C007262:deoxynivalenol
Metod opredeleniia dezoksinivalenola (vomitoksina) i ego metabolitov.