Electrophoretic analysis of neuronal genomic DNA from hypertrophic spinal ganglia during lizard tail regeneration.
Cytoplasmic and nuclear hypertrophy in neurons from the last 3 pairs of sensory ganglia left in situ cranially to the plane of amputation occurs during lizard tail regeneration. Cytophotometry after Feulgen staining demonstrated the presence of some neurons, from hypertrophic ganglia, whose quantity of DNA exceeded the diploid level (hyperdiploid neurons). In the present work agarose gel electrophoresis of total genomic DNA extracted from hypertrophic ganglia showed one or two bands migrating below the high molecular weight DNA, pointing to a selective amplification of discrete DNA segments.
Borrione
P
P
Dipartimento di Anatomia e Fisiologia Umana, Universitá di Torino, Italy.
Cervella
P
P
Geuna
S
S
Giacobini-Robecchi
M G
MG
Poncino
A
A
Silengo
L
L
D000671:Amputation, Surgical; D000818:Animals; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005727:Ganglia, Spinal / Q000473:pathology / Q000502:physiology*; D016678:Genome; D006984:Hypertrophy; D008116:Lizards / Q000502:physiology*; D009474:Neurons / Q000473:pathology / Q000502:physiology*; D012016:Reference Values; D012038:Regeneration; D015183:Restriction Mapping; D013623:Tail / Q000502:physiology*
D004247:DNA