title: [Isolation of recombinant interleukin-3 produced by E. coli].
abstract: A synthetic gene coding for human interleukin-3 (hIL3) was cloned in the plasmid pTE2IL3, the gene expression being controlled by the phage fd PVIII promotor and the phage T7 gene 10 translational enhancer. Under constitutive biosynthesis conditions in E. coli, the accumulation of recombinant hIL3 (in the inclusion bodies) was up to 30-40% of the total cell protein. An effective procedure of the hIL3 isolation is suggested. The hIL3 was solubilized in 5 M guanidinium chloride, renaturated and purified to homogeneity by a single chromatographic step. The protein's yield was 34 mg/g wet cells. The isolated hIL3 showed a specific biological activity.
authors.forename: S V
authors.initials: SV
authors.lastname: Lutsenko
authors.forename: A I
authors.initials: AI
authors.lastname: Gurevich
authors.forename: V Iu
authors.initials: VIu
authors.lastname: Kanevskiĭ
authors.forename: V A
authors.initials: VA
authors.lastname: Smirnov
authors.forename: I V
authors.initials: IV
authors.lastname: Nazimov
authors.forename: T L
authors.initials: TL
authors.lastname: Azhikina
authors.forename: I P
authors.initials: IP
authors.lastname: Chernov
authors.forename: V M
authors.initials: VM
authors.lastname: Rostapshov
authors.forename: N V
authors.initials: NV
authors.lastname: Sonina
authors.forename: A V
authors.initials: AV
authors.lastname: Azhaev
mesh_terms: D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004742:Enhancer Elements, Genetic; D004926:Escherichia coli / Q000378:metabolism*; D015870:Gene Expression; D005814:Genes, Viral; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D007377:Interleukin-3 / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D010957:Plasmids; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects; D013604:T-Phages / Q000235:genetics
chemical_list: D007377:Interleukin-3; D011994:Recombinant Proteins; C074582:9-(1'-hydroxy-2'-(hydroxymethyl)ethoxy)methylguanine; D006147:Guanine
vernacular_title: Vydelenie rekombinantnogo interleĭkina-3, produtsiruemogo E. coli.