A rapid procedure for detecting recombinant plasmids using butanol extraction.
A convenient procedure using butanol to extract DNA from bacteria is described. This procedure is suitable for rapidly screening a large number of bacterial cultures for recombinant plasmid DNA without the need for further purification and expensive chemicals.
Mak
Y M
YM
Botany Department, National University of Singapore, Republic of Singapore.
Sornarajah
R
R
Ho
K K
KK
D000440:Butanols; D004269:DNA, Bacterial / Q000032:analysis*; D004274:DNA, Recombinant / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D010957:Plasmids
D000440:Butanols; D004269:DNA, Bacterial; D004274:DNA, Recombinant