Ultrastructural localization of Ca2+ in the Golgi apparatus of two different cell types.
The precipitation reaction with potassium oxalate was used to investigate the subcellular localization of Ca2+ in atrial myocardocytes and in Schwann cells of non-myelinated nerve fibers. Chelation with EGTA was used to prove the presence of Ca2+ in the electronoopaque deposits. In both cell types the oxalate reaction was intense in the classically described Ca2+ deposits: the perinuclear cisterna, the endo (sarco) plasmic reticulum, the mitochondrial matrix, but also in a novel localization: the cisternae of the Golgi stack in all cis to trans positions, suggesting that Golgi Apparatus is a major intracellular Ca2+ pool.
Vidulescu
C
C
Department of Cell Biology and Histology, Faculty of Medicine, Bucharest.
Onicescu
D
D
Popescu
M
M
Martin
S
S
D000818:Animals; D002118:Calcium / Q000032:analysis*; D011232:Chemical Precipitation; D006056:Golgi Apparatus / Q000737:chemistry* / Q000648:ultrastructure; D006168:Guinea Pigs; D008297:Male; D008854:Microscopy, Electron; D009206:Myocardium / Q000648:ultrastructure*; D010070:Oxalates; D051381:Rats; D012583:Schwann Cells / Q000648:ultrastructure*
D010070:Oxalates; D002118:Calcium