Effects of anisodamine and dauricine on proliferation, DNA synthesis, and calcium influx in bovine anterior cerebral arterial smooth muscle cells in culture.
The in vitro culture of bovine anterior cerebral arterial smooth muscle cells (BACASMC) was first established in our laboratory. Anisodamine and dauricine inhibited the proliferation, DNA synthesis and calcium influx in the cells in dose-dependent manners. At 0.01 mmol.L-1, both drugs inhibited the proliferation by 17.6% and 8.3%, the DNA synthesis by 11.9% and 56.8%, the calcium influx by 26.6% and 31.4%, respectively. The results indicated that anisodamine and dauricine might have prospect in the prevention and treatment of cerebrovascular diseases.
Zeng
G Q
GQ
Department of Pharmacology, College of Pharmacy, Second Military Medical University, Shanghai, China.
Rui
Y C
YC
D000470:Alkaloids; D000818:Animals; D044182:Benzylisoquinolines; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002536:Cerebral Arteries / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis*; D007546:Isoquinolines / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012991:Solanaceous Alkaloids / Q000494:pharmacology*; D044005:Tetrahydroisoquinolines
D000470:Alkaloids; D044182:Benzylisoquinolines; D002121:Calcium Channel Blockers; D007546:Isoquinolines; D012991:Solanaceous Alkaloids; D044005:Tetrahydroisoquinolines; C003922:anisodamine; C035934:dauricine; D004247:DNA; D002118:Calcium