[Effect of colchicine on vasopressin and melanin-concentrating hormone neurons of rat hypothalamus: hybridocytochemistry and immunocytochemistry studies].
48 hrs. after an intra-cerebroventricular injection of colchicine (100 micrograms), antisera to three putative peptides included in the rat melanin-concentrating hormone (MCH) precursor, strongly stained the secretory granules accumulated in perikarya. In control rats, these antisera stained endoplasmic reticulum, Golgi apparatus, or neurosecretory granules respectively. Colchicine also induced a dramatic decrease in hybridization signal obtained with a probe complementary to the prepro-MCH-mRNA. Similarly, colchicine induced a strong increase in vasopressin immunoreactivity in neurons of the paraventricular and supraoptic nuclei, and a strong decrease of the vasopressin precursor mRNA. These results demonstrated that, in two peptidergic neuron populations of the rat hypothalamus, colchicine lowers mRNAs and impairs neuropeptide protein synthesis, consecutively to the accumulation of neurosecretory granules in perikarya.
Risold
P Y
PY
Laboratoire d'Histologie Embryologie cytogénétique, C.N.R.S.-U.R.A. n. 0561, Faculté de Médecine et de Pharmacie, Besançon.
Fellmann
D
D
Bugnon
C
C
D000818:Animals; D003078:Colchicine / Q000494:pharmacology*; D003594:Cytoplasmic Granules / Q000378:metabolism; D007028:Hypothalamic Hormones; D007031:Hypothalamus / Q000166:cytology*; D007150:Immunohistochemistry; D008297:Male; D008543:Melanins / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization / Q000187:drug effects; D010907:Pituitary Hormones / Q000378:metabolism*; D015347:RNA Probes / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D014667:Vasopressins / Q000378:metabolism*
D007028:Hypothalamic Hormones; D008543:Melanins; D010907:Pituitary Hormones; D015347:RNA Probes; D014667:Vasopressins; C019463:melanin-concentrating hormone; D003078:Colchicine
Effet de la colchicine sur les neurones à vasopressine et à hormone de mélano-concentration de l'hypothalamus du rat: études hybridocytochimique et immunocytochimique.