Effect of an in vivo immunostimulant treatment on PMN functions: interaction with antibiotics in vitro.
The in vivo effect of immunostimulant treatment, bacterial ribosomes together with membranal proteoglycans (three tablets and three sprays daily during 4 days) in combination or not with in vitro amoxicillin or josamycin (range 5-50 micrograms/ml), has been studied on three related functions of polymorphonuclear cells (PMN) isolated from human blood: adhesion, spontaneous and oriented migration. The immunostimulant treatment always induces an increase of the three parameters. A positive effect of high concentrations of amoxicillin could be also observed on adhesion and gives rise to a great additional impact by associating it with immunostimulant treatment (control: 8.26 PMN/field; assay: 51.55 PMN/field). Spontaneous and oriented migration are not very altered by in vitro antibiotics. A very significant decrease of chemotaxis has been only observed with 50 microgram/ml of josamycin. However, the immunostimulant treatment compensates for this negative effect. The real increase in PMN activation produced by the immunostimulant treatment might extend its indications beyond prevention during antibiotherapy, for example in the acute phase of respiratory infections.
Roques
C
C
Faculté des Sciences Pharmaceutiques, Laboratoire de Bactériologie, Virologie et Microbiologie Industrielle, Toulouse, France.
Frayret
M N
MN
Luc
J
J
Michel
G
G
Perruchet
A M
AM
Cauquil
J
J
Levy
D
D
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000328:Adult; D000658:Amoxicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000942:Antigens, Bacterial / Q000008:administration & dosage; D002448:Cell Adhesion / Q000187:drug effects / Q000276:immunology; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000276:immunology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D015570:Josamycin / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology* / Q000502:physiology
D000276:Adjuvants, Immunologic; D000900:Anti-Bacterial Agents; D000942:Antigens, Bacterial; C034206:Ribomunyl; D000658:Amoxicillin; D015570:Josamycin