The synthesis of oligonucleotides that contain 2,4-dinitrophenyl reporter groups.
The synthesis of non-nucleoside-based phosphoramidites that bear the 2,4-dinitrophenyl group is reported. These labelled phosphoramidites, which have been used in solid-phase oligonucleotide synthesis to attach single and multiple dinitrophenyl groups to the 5'-end of oligonucleotides, are entirely compatible with the normal oligonucleotide synthesis cycle. Multiple labelling can be performed readily in high yield and the resulting oligonucleotides can be purified readily by reversed-phase h.p.l.c. The labelled oligonucleotides have been detected using monoclonal and polyclonal anti-dinitrophenyl antibodies.
Will
D W
DW
Department of Chemistry, University of Edinburgh, Great Britain.
Pritchard
C E
CE
Brown
T
T
D019297:2,4-Dinitrophenol; D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry; D004140:Dinitrophenols / Q000737:chemistry; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry
D004140:Dinitrophenols; D009838:Oligodeoxyribonucleotides; D011089:Polydeoxyribonucleotides; D004247:DNA; D019297:2,4-Dinitrophenol