title: Rapid postmortem decrease in the ectocellular acetylcholinesterase activity in rat striatum as assessed by in vivo microspectrophotometry.
abstract: The acetylcholinesterase (AChE) activity in striatum rat was determined before and shortly after death using the in vivo microspectrophotometric method. This technique allowed us to monitor the Ellman colorimetric reaction directly inside the brain using an optical probe implanted in a live animal and to determine locally the AChE activity. Whatever the cause of the animals death, we observed a drastic postmortem decrease of the AChE activity of about 35-50%, 10 min after death. We have verified that the postmortem decrease of brain temperature or pH and postmortem optical properties changes could only explain a fraction of the AChE activity fall (16%). This phenomenon seems to be related to events strictly localized at the cellular level, since local injection of cyanide at the measuring site promotes a decrease of the enzymatic activity (40%) close to the levels observed after death. The origin of this rapid postmortem fall of the AChE activity is discussed. The technical properties of the microspectrophotometric method exclude a decrease of the ectocellular pool of enzyme after death. Our results allow us to envisage the existence of an in vivo endogenous regulation of the AChE activity which disappears shortly after death.
authors.affiliation: Centre de Recherches du Service de Santé des Armées, Grenoble, France.
authors.forename: G
authors.initials: G
authors.lastname: Testylier
authors.forename: P
authors.initials: P
authors.lastname: Gourmelon
authors.forename: D
authors.initials: D
authors.lastname: Clarençon
authors.forename: E
authors.initials: E
authors.lastname: Multon
authors.forename: M
authors.initials: M
authors.lastname: Fatome
authors.forename: J
authors.initials: J
authors.lastname: Viret
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000201:enzymology*; D003643:Death; D004622:Embryo, Mammalian; D005110:Extracellular Space / Q000201:enzymology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009474:Neurons / Q000201:enzymology*; D011180:Postmortem Changes; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry / Q000379:methods
chemical_list: D000110:Acetylcholinesterase