Enzyme variants of Eimeria parasitizing the domestic fowl and possibilities of species diagnostics.
Electrophoretic variation of the enzymes lactate dehydrogenase (LDH) and glucosephosphate isomerase (GPI) of Eimeria parasitizing the domestic fowl in Czechoslovakia is summarized and the differentiation of species of poultry coccidia is discussed. A new method for evaluation of zymograms of coccidial enzymes is presented. This method enables the results of different experiments to be compared by calculating standardized rates of mobility of each enzyme band relative to the positions of reference variants coded LDH-8 or GPI-9.
Kucera
J
J
Research Institute for Feed Supplements and Veterinary Drugs, Prague, Czechoslovakia.
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary*; D003604:Czechoslovakia; D004539:Eimeria / Q000145:classification* / Q000201:enzymology; D004592:Electrophoresis, Starch Gel; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008297:Male; D011200:Poultry; D011201:Poultry Diseases / Q000469:parasitology*; D012189:Retrospective Studies
D007770:L-Lactate Dehydrogenase; D005956:Glucose-6-Phosphate Isomerase