title: [Preservation of hearts by perfusion under pressure].
abstract: OBJECTIVE
  Development of method used to fix hearts in a reduced time, with preservation of their structures and characteristics.
  
  
  METHODS
  Fifty normal human hearts were fixed in a system with four reservoirs, a roller pump, PVC tubes and a plastic recipient. This system was used to maintain the continuous injection by pressure of formaldehyde solution, to the interior of the heart.
  
  
  RESULTS
  The fixation was satisfactory, pré-fixation time (13.93 +/- 5.13 min) and fixation time (60.53 +/- 12.50 min) were reduced related to previously utilized methods.
  
  
  CONCLUSIONS
  The method was good, with decrease of fixation time and obtention of fixed hearts, with preservation of their structures and characteristics.
authors.affiliation: Instituto do Coração do Hospital das Clínicas-FMUSP, São Paulo.
authors.forename: F B
authors.initials: FB
authors.lastname: Jatene
authors.forename: M H
authors.initials: MH
authors.lastname: Magalhães
authors.forename: P R
authors.initials: PR
authors.lastname: De Oliveira
authors.forename: A D
authors.initials: AD
authors.lastname: Jatene
mesh_terms: D000447:Aldehydes; D015925:Cryopreservation / Q000379:methods*; D006321:Heart; D006801:Humans; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion / Q000379:methods*; D011312:Pressure; D013997:Time Factors
chemical_list: D000447:Aldehydes
vernacular_title: Técnica de preservação de corações através de perfusão sob pressão.