title: The use of negative ion thermospray liquid chromatography/tandem mass spectrometry for the determination of bile acids and their glycine conjugates.
abstract: A study was carried out on the negative ion thermospray liquid chromatography/mass spectrometry and liquid chromatography/tandem mass spectrometry of a group of bile acids and their glycine conjugates. The liquid chromatographic/tandem mass spectrometric experiments were performed using low-energy collisions on a triple-quadrupole mass spectrometer. It was found that relatively high collision energies and collision gas pressures were required to produce fragmentation and that some unusual fragments were produced.
authors.affiliation: Smith Kline & French Research Ltd, Welwyn, Herts, UK.
authors.forename: C
authors.initials: C
authors.lastname: Eckers
authors.forename: P B
authors.initials: PB
authors.lastname: East
authors.forename: N J
authors.initials: NJ
authors.lastname: Haskins
mesh_terms: D002635:Chenodeoxycholic Acid / Q000032:analysis*; D019826:Cholic Acid; D002793:Cholic Acids / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D003840:Deoxycholic Acid / Q000032:analysis*; D005999:Glycochenodeoxycholic Acid / Q000032:analysis*; D006000:Glycocholic Acid / Q000032:analysis*; D006002:Glycodeoxycholic Acid / Q000032:analysis*; D008095:Lithocholic Acid / Q000031:analogs & derivatives* / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods
chemical_list: D002793:Cholic Acids; D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; D006002:Glycodeoxycholic Acid; D008095:Lithocholic Acid; D005999:Glycochenodeoxycholic Acid; D019826:Cholic Acid; D006000:Glycocholic Acid; C027746:glycolithocholic acid