Development of the Abbott MATRIX Aero assay for the measurement of specific IgE.
An enzyme immunoassay has been developed for the quantitation of specific immunoglobulin E (IgE) in human serum to a panel of allergens. The assay system, called the Abbott MATRIX Aero, includes an instrument, reagents and test cell disposables. Each test cell contains fourteen airborne allergens individually localized on a nitrocellulose solid phase. Individual calibration curves for each allergen are established by the manufacturer and included in barcode form with each test kit. Stable factory calibration eliminates the need to establish a calibration curve with each assay run. The instrument automatically incubates, washes, and reads the test cell and prints each result, which ensures assay reproducibility and provides ease-of-use. Analysis of test results shows good agreement with another in vitro assay for specific IgE. The Abbott MATRIX Aero is a sensitive, reproducible and easy-to-use system for the measurement of specific IgE to a panel of fourteen allergens simultaneously using a single, small volume of serum.
Lindberg
R E
RE
Abbott Laboratories, Abbott Park, IL 60064-3500.
Anawis
M A
MA
Bailey
M
M
Mangat
D
D
Frank
P M
PM
Hrusovsky
I G
IG
Hooyman
L
L
Putterman
C
C
Defreese
J D
JD
D000485:Allergens / Q000276:immunology; D000918:Antibody Specificity; D002138:Calibration; D005069:Evaluation Studies as Topic; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis*; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology; D011852:Radioallergosorbent Test; D011933:Reagent Kits, Diagnostic / Q000592:standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000485:Allergens; D011933:Reagent Kits, Diagnostic; D007073:Immunoglobulin E