Botulinum neurotoxin type A: structure and interaction with the micellar concentration of SDS determined by FT-IR spectroscopy.
Secondary structures of botulinum neurotoxin type A have been determined using Fourier transform infrared spectroscopy in the amide I and amide III frequency regions. Using Fourier self-deconvolution, second derivatization, and curve-fit analysis, the amide I frequency contour was resolved into Gaussian bands at 1678, 1654, 1644, and 1634 cm-1. In the amide III frequency region, several small bands were resolved between 1320 and 1225 cm-1. Assignments of the bands in both amide I and amide III frequency regions to various types of secondary structures and the estimation of spectral band strengths by integrating areas under each band suggested that the neurotoxin contains 29% alpha-helix, 45-49% beta-sheets and 22-26% random coils. These values agreed very well with those determined earlier from CD spectra. The neurotoxin was treated with a micellar concentration of sodium dodecyl sulfate to simulate interaction between the protein and the amphipathic molecules. Sodium dodecyl sulfate micelles induced significant alterations both in the spectral band positions, and their strengths suggest refolding of the neurotoxin polypeptides. However, these changes were not entirely reversible, which could implicate the role of the altered structures in the function of the neurotoxin.
Singh
B R
BR
Food Research Institute, University of Wisconsin, Madison 53706.
Fuller
M P
MP
DasGupta
B R
BR
D001905:Botulinum Toxins / Q000378:metabolism*; D002942:Circular Dichroism; D005583:Fourier Analysis; D008051:Lipid Bilayers; D008823:Micelles; D011487:Protein Conformation; D012967:Sodium Dodecyl Sulfate / Q000378:metabolism*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D008051:Lipid Bilayers; D008823:Micelles; D012967:Sodium Dodecyl Sulfate; D001905:Botulinum Toxins