Inhibition of high-affinity [3H]L-proline binding to rat brain membranes by 2-amino-7-phosphonoheptanoic acid.
L-Glutamate and 2-amino-7-phosphonoheptanoic acid [corrected] (AP-7) (10(-9) to 10(-6) M), a competitive NMDA (N-methyl-D-aspartate) antagonist, inhibit approximately 60% of [3H]L-proline binding to rat membranes from midbrain. In hippocampal membranes, AP-7 inhibits proline binding by 80%, while in cerebellar membranes AP-7 had little effect. These results are indicative of the possible neuromodulatory role of proline in the central nervous system, possibly through the NMDA receptor(s).
Cordero
M L
ML
Department of Pharmacology, University of Puerto Rico School of Medicine, San Juan 00936-5067.
Negrón
A E
AE
Ortiz
J G
JG
Blanco
C
C
Santiago
G
G
D015763:2-Amino-5-phosphonovalerate / Q000031:analogs & derivatives*; D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D008566:Membranes / Q000378:metabolism; D011392:Proline / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D000596:Amino Acids; D014316:Tritium; D015763:2-Amino-5-phosphonovalerate; D011392:Proline; C031231:2-amino-7-phosphonoheptanoic acid