[Changes in erythrocyte membrane proteins in patients with cirrhosis].
The serum and erythrocyte membrane proteins of patients with cirrhosis were studied. Recent work on abnormalities of the erythrocyte membrane resulted in the identification of several types of membrane skeleton lesions. The different techniques for separation of membrane proteins have been revised and compared. These were studied on slabs using the SDS-polyacrylamide gel and discontinuous buffer system with a linear concentration gradient of 5-15% (w/v). Serum proteins were separated by using continuous buffer system with a linear concentration gradient of 4-30%. A decreased in serum and red cell membrane proteins in those patients, were observed.
Méndez-Marco
M T
MT
Centro Mixto C.S.I.C.-U.C.M., Facultad de Farmacia, Ciudad Universitaria, Madrid.
Arce de Obieta
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C
Palacios-Alaiz
E
E
D000743:Anemia, Hemolytic / Q000097:blood / Q000209:etiology; D001797:Blood Protein Electrophoresis / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis*; D002021:Buffers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004910:Erythrocyte Membrane / Q000737:chemistry*; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood* / Q000150:complications; D008565:Membrane Proteins / Q000097:blood*; D008970:Molecular Weight
D001798:Blood Proteins; D002021:Buffers; D008565:Membrane Proteins
Variaciones de las proteínas de membrana de eritrocito en pacientes con cirrosis.