Oligonucleotide-directed DNA triple-helix formation: an approach to artificial repressors?
Pyrimidine oligonucleotides exhibit sequence-specific binding to purine sequences in double-helical DNA. Molecular recognition occurs through triple-helix formation, mediated by Hoogsteen hydrogen bonding of the pyrimidine strand parallel to the double-helical DNA purine strand. Oligonucleotide-directed DNA triple-helix formation has been shown to inhibit the recognition of double-helical DNA by sequence-specific binding proteins. This observation provides the potential for new approaches to both the analysis of nucleic acids, and the study of nucleic acid-protein interactions. We wish to understand molecular mechanisms whereby such complexes affect protein-DNA interactions involved in eukaryotic transcription.
Maher
L J
LJ
Division of Chemistry, California Institute of Technology, Pasadena 91125.
Wold
B
B
Dervan
P B
PB
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D006860:Hydrogen Bonding; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016376:Oligonucleotides, Antisense; D011743:Pyrimidines
D004268:DNA-Binding Proteins; D016376:Oligonucleotides, Antisense; D011743:Pyrimidines; D004247:DNA