title: Optical separation of racemic 5-(p-hydroxyphenyl)-5-phenylhydantoin by reversed phase high-performance liquid chromatography using eluents containing beta-cyclodextrin.
abstract: Both S-(-)- and R-(+)-enantiomers of 5-(p-hydroxyphenyl)-5-phenylhydantoin (p-HPPH), a main oxidative metabolite of the achiral antiepileptic drug phenytoin, could be determined simply, sensitively and accurately using reversed phase high-performance liquid chromatography by using a methanol-monopotassium phosphate eluent containing beta-cyclodextrin. Using this assay procedure, it was determined that an S-(-)-enantiomer was formed predominantly by the oxidation of phenytoin in isolated rat hepatocytes.
authors.affiliation: Department of Hospital Pharmacy, School of Medicine, University of Occupational and Environmental Health, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Eto
authors.forename: H
authors.initials: H
authors.lastname: Noda
authors.forename: M
authors.initials: M
authors.lastname: Minemoto
authors.forename: A
authors.initials: A
authors.lastname: Noda
authors.forename: Y
authors.initials: Y
authors.lastname: Mizukami
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000737:chemistry*; D008099:Liver / Q000737:chemistry / Q000166:cytology; D010672:Phenytoin / Q000031:analogs & derivatives* / Q000302:isolation & purification; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: D003505:Cyclodextrins; C027364:5-(3-hydroxyphenyl)-5-phenylhydantoin; D010672:Phenytoin