Cytotoxic action of triphenyltin on mouse thymocytes: a flow-cytometric study using fluorescent dyes for membrane potential and intracellular Ca2+.
Effects of triphenyltin on mouse thymocytes were examined using fluorescent dyes to monitor membrane potential and intracellular Ca2+. Triphenyltin at 3 x 10(-7) M to 1 x 10(-6) M hyperpolarized thymocytes and depolarized them at 3 x 10(-6) M or more, associated with increasing intracellular Ca2+. Hyperpolarization was suppressed by quinine, but not by tetraethylammonium and 4-aminopyridine, suggesting the involvement of Ca(2+)-activated K+ current. Triphentyltin failed to hyperpolarize thymocytes in Ca(2+)-free solution. Results indicate that triphenyltin promotes Ca(2+)-influx to thymocytes. Such an action of triphenyltin may be related to the immunotoxicity of organotins.
Oyama
Y
Y
Department of Health Science, Faculty of Integrated Arts and Sciences, University of Tokushima, Japan.
Chikahisa
L
L
Tomiyoshi
F
F
Hayashi
H
H
D015761:4-Aminopyridine / Q000494:pharmacology; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D005434:Flow Cytometry; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D007424:Intracellular Fluid / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D009947:Organotin Compounds / Q000633:toxicity*; D015221:Potassium Channels / Q000187:drug effects; D011803:Quinine / Q000494:pharmacology; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
D005456:Fluorescent Dyes; D009947:Organotin Compounds; D015221:Potassium Channels; D013757:Tetraethylammonium Compounds; D000001:Calcimycin; D019789:Tetraethylammonium; C030665:triphenyltin; D011803:Quinine; D015761:4-Aminopyridine; D002118:Calcium