title: [An effective method for site-directed mutagenesis in plasmids and cloning single-stranded DNA fragments].
abstract: A three primer variant of the earlier devised oligonucleotide-directed mutagenesis in plasmids is described, useful also for the fast cloning of single-stranded DNA products of the asymmetric polymerase chain reaction (PCR). Using this method for plasmid pHD-001-14-11, a 59 b. p. deletion and a 7 b. p. insertion were simultaneously introduced at 81% frequency, and the PCR-copied phage fd transcription terminator (26 b. p.) was inserted with the yield of 67%.
authors.forename: V L
authors.initials: VL
authors.lastname: Drutsa
authors.forename: V R
authors.initials: VR
authors.lastname: Kaberdin
authors.forename: O N
authors.initials: ON
authors.lastname: Koroleva
authors.forename: I A
authors.initials: IA
authors.lastname: Shilov
mesh_terms: D001345:Autoradiography; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004277:DNA, Single-Stranded / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D016133:Polymerase Chain Reaction; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D004277:DNA, Single-Stranded
vernacular_title: Effektivnyĭ metod napravlennogo vvedeniia mutatsiĭ v plazmidy i klonirovaniia odnotiazhevykh fragmentov DNA.