[Enrichment for mutants of Bacillus megaterium deficient in the synthesis of poly-beta-hydroxybutyrate (PHB)].
Centrifugation through sucrose gradients was adapted to separate spore-forming cells of B. megaterium deficient in poly-beta-hydroxybutyrate synthesis from wild type cells. The conditions described allowed the detection of mutant clones screening a low percentage of the mutagenized population.
Floccari
M E
ME
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Tiscornia
G
G
Palmada
F
F
Méndez
B S
BS
D001410:Bacillus megaterium / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D006885:Hydroxybutyrates / Q000378:metabolism*; D011091:Polyesters / Q000378:metabolism*; D013171:Spores, Bacterial; D000094122:Polyhydroxybutyrates
D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
Enriquecimiento en mutantes de Bacillus megaterium deficientes en la síntesis de poli-beta-hidroxibutirato (PHB).