[The characteristics of the course of mitosis in polykaryons formed during cell fusion].
A new method of cell fusion is proposed utilizing treatment with 15% solution of DMSO in serum before and after PEG treatment. With such treatments in SPEV cell culture a higher rate of cell fusion was obtained than that with other known methods of cell fusion. In the first wave of mitoses (0.5-4 h) mainly asynchronous division of nuclei, premature chromosome condensation and formation of telophase-like nuclei were observed in polykaryons. In the period of the second wave (14-20 h), mitoses were mainly synchronous and completed with cytokinesis. Micronuclei were formed frequently as a result of such mitoses. After the first wave of mitoses the number of polykaryons with pycnotic chromosomes sharply increased, and after the second wave of mitoses the number of polykaryons with pycnotic nuclei increased. The results obtained allow to conclude that heterophasic condition of the fused cells is one of the causes of pathological mitosis of polykaryons and of their death.
Manandhar
G
G
Khodiakov
A L
AL
Onishchenko
G E
GE
D000818:Animals; D002459:Cell Fusion; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured / Q000648:ultrastructure; D003584:Cytological Techniques; D015726:Giant Cells / Q000648:ultrastructure*; D008938:Mitosis; D013997:Time Factors
Osobennosti protekaniia mitoza v polikarionakh, obrazuiushchikhsia pri sliianii kletok.