Isolation and characterization of a lethal phospholipase A2 (NN-IVb1-PLA2) from the Indian cobra (Naja naja naja) venom.
Indian cobra (Naja naja naja) venom is reported to contain multiple forms of phospholipase A2. Only a couple of them have been isolated and characterized. A lethal phospholipase A2 (NN-IVb1-PLA2) from Naja naja naja venom has been purified in three steps involving CM-Sephadex C-25, Sephadex G-50 and rechromatography on CM-Sephadex C-25 columns. It is a basic protein with pl value between 7-7.5 and has molecular weight between 11,000-11,500. The LD50 of NN-IVb1-PLA2 is 1.2 mg/K g body weight. It induces neurotoxic symptoms in the experimental mice and is devoid of myotoxic, anticoagulant, edema inducing and direct hemolytic activities.
Bhat
M K
MK
Department of Studies in Biochemistry, University of Mysore, Manasagangotri, India.
Gowda
T V
TV
D000818:Animals; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D004546:Elapid Venoms / Q000737:chemistry / Q000201:enzymology* / Q000302:isolation & purification; D007928:Lethal Dose 50; D051379:Mice; D008970:Molecular Weight; D010741:Phospholipases A / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D054467:Phospholipases A2
D004546:Elapid Venoms; D010741:Phospholipases A; D054467:Phospholipases A2