Linkage of the chicken MHC to the nucleolus organizer region visualized using non-isotopic in situ hybridization.
Linkage of the chicken major histocompatibility complex (B-complex) to the nucleolus organizer regions (NOR) has been confirmed in situ. Nonradioactive hybridization to metaphase chromosomes using a pool of biotinylated cDNA probes for the B-F, B-L beta, and B-G genes of the B-complex gave positive signals on one or a pair of microchromosomes. Subsequent staining with silver nitrate proved these microchromosomes to be the NOR-bearing pair. The value of employing nonisotopic in situ hybridization techniques for mapping of genes and gene complexes, especially when dealing with microchromosomes, is stressed.
Dominguez-Steglich
M
M
Department of Human Genetics, University of Würzburg, Germany.
Auffray
C
C
Schmid
M
M
D000818:Animals; D001710:Biotin; D002645:Chickens / Q000235:genetics*; D002874:Chromosome Mapping; D015342:DNA Probes; D008040:Genetic Linkage; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008677:Metaphase; D009697:Nucleolus Organizer Region; D012835:Silver Nitrate
D015342:DNA Probes; D001710:Biotin; D012835:Silver Nitrate