title: Reversed-phase liquid chromatographic method for the simultaneous determination of the antimalarial drugs sulfadoxine, pyrimethamine, mefloquine and its major carboxylic metabolite in plasma.
abstract: A high-performance liquid chromatographic (HPLC) method for the simultaneous determination of sulfadoxine, pyrimethamine, mefloquine and the carboxylic metabolite of mefloquine in plasma is described. After the proteins have been precipitated with a combination of zinc sulphate and acetonitrile containing two internal standards, pyrimethamine and mefloquine are extracted as bases and sulfadoxine and the carboxylic metabolite of mefloquine as ion-pairs with tetrabutylammonium. The drugs are separated by HPLC on a 3 micron octadecylsilica column with ultraviolet detection at 229 nm. The method is simple and reliable and enables the simultaneous determination of the drugs in 600-microliter plasma samples with a sensitivity suitable for standard drug monitoring purposes.
authors.affiliation: Department of Clinical Chemistry, Falun Central Hospital, Sweden.
authors.forename: Y
authors.initials: Y
authors.lastname: Bergqvist
authors.forename: S
authors.initials: S
authors.lastname: Eckerbom
authors.forename: H
authors.initials: H
authors.lastname: Larsson
authors.forename: M
authors.initials: M
authors.lastname: Malekzadeh
mesh_terms: D000962:Antimalarials / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015767:Mefloquine / Q000097:blood; D011739:Pyrimethamine / Q000097:blood; D013413:Sulfadoxine / Q000097:blood*
chemical_list: D000962:Antimalarials; D013413:Sulfadoxine; D015767:Mefloquine; D011739:Pyrimethamine