title: A two-step purification procedure for sheep liver 6-phosphogluconate dehydrogenase.
abstract: A two-step procedure for the purification of 6-phosphogluconate dehydrogenase (EC 1.1.1.44; 6-PGDH) from sheep liver is described. The enzyme is directly bound to cellulose phosphate by batch extraction and eluted with a linear salt gradient. Purification is completed by affinity chromatography using NADP(+)-agarose. The result is 6-PGDH of high purity, greatly increased yield, and the highest specific activity yet achieved, with a significant reduction in the purification time.
authors.affiliation: Laboratory of Molecular Biophysics, University of Oxford, United Kingdom.
authors.forename: D O
authors.initials: DO
authors.lastname: Somers
authors.forename: J
authors.initials: J
authors.lastname: Hajdu
authors.forename: M J
authors.initials: MJ
authors.lastname: Adams
mesh_terms: D000818:Animals; D002482:Cellulose / Q000031:analogs & derivatives; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003956:Dialysis; D008099:Liver / Q000201:enzymology*; D010734:Phosphogluconate Dehydrogenase / Q000302:isolation & purification*; D012756:Sheep
chemical_list: D002482:Cellulose; C008881:phosphocellulose; D010734:Phosphogluconate Dehydrogenase