title: Absorption and metabolic characteristics of p-aminobenzoic acid and its isomer, m-aminobenzoic acid, from the rat small intestine.
abstract: Absorption and metabolic characteristics of p-aminobenzoic acid (PABA) and m-aminobenzoic acid (MABA) from the rat small intestine were examined by means of in situ recirculation and in vitro everted sac experiments. p-Aminobenzoic acid was extremely rapidly absorbed from the rat small intestine, whereas the absorption of MABA, the m-isomer of PABA, was comparably slower. This finding was partly explained by the result that PABA is more lipophilic than MABA. The metabolite percentage of PABA was considerably greater than that of MABA in mucosal fluid, tissue, and serosal fluid. On the other hand, a concentration-dependent and a directional difference in the transfer rate of these drugs were observed in everted and noneverted sacs of rat small intestine. Furthermore, mucosal uptake of PABA or MABA was inhibited by 1 mM 2,4-dinitrophenol, 10 mM sodium azide, and pretreatment with HgCl2 (10 mM). These results indicate that MABA, as well as PABA, is transported through the intestine by a carrier-mediated transport system, and that the molecular structure of these drugs is important for their absorption and metabolic characteristics.
authors.affiliation: Department of Basic Pharmaceutics, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Sakane
authors.forename: M
authors.initials: M
authors.lastname: Shibukawa
authors.forename: M
authors.initials: M
authors.lastname: Hashida
authors.forename: H
authors.initials: H
authors.lastname: Sezaki
mesh_terms: D010129:4-Aminobenzoic Acid / Q000378:metabolism / Q000493:pharmacokinetics*; D062365:Aminobenzoates / Q000378:metabolism / Q000493:pharmacokinetics*; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000502:physiology*; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013237:Stereoisomerism; D062368:meta-Aminobenzoates
chemical_list: D062365:Aminobenzoates; D062368:meta-Aminobenzoates; C044855:3-aminobenzoic acid; D010129:4-Aminobenzoic Acid