The Yucatan miniature swine as an in vivo model for screening skin depigmentation.
The usefulness of the Yucatan miniature pig as a screen for skin dipigmenting activity by topical application was evaluated with standard compounds. This is a naturally occurring breed of swine with light brown to dark brown skin that is relatively hairless. The skin morphology, including the pattern of pigment distribution, in this breed of swine closely resembles the human skin. Test compounds examined in this study included the three standard compounds with known clinical depigmenting activity, hydroquinone (HQ), 4-hydroxyanisole (4HA) and tert-butyl catechol (TBC), each at a 5% concentration. Test materials in 25 microliters of propylene glycol/ethanol (50:50) were applied topically twice daily, 7 days a week for 90 days to test sites on each side of the dorsal mid-line. Test sites were graded weekly for variation in pigmentation and local irritation. After 90 days of test material application, skin biopsies of the test sites were taken for histological evaluation. Topical application of HQ, 4HA and TBC promoted marked skin depigmentation which was substantiated by reductions of pigment and melanocytes observed on microscopic examination. While both HQ and TBC produced marked local irritation, 4HA was only mildly irritating. These results suggest that the Yucatan pig, could be a potentially useful model for screening compounds with skin depigmenting activity.
Nair
X
X
Bristol-Myers Squibb Co., Pharmaceutical Research Institute, Buffalo, NY 14213.
Tramposch
K M
KM
D000818:Animals; D000840:Anisoles / Q000494:pharmacology; D002396:Catechols / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D005260:Female; D006873:Hydroquinones / Q000494:pharmacology; D008954:Models, Biological; D012867:Skin / Q000187:drug effects / Q000473:pathology; D012880:Skin Pigmentation / Q000187:drug effects*; D013552:Swine; D013556:Swine, Miniature
D000840:Anisoles; D002396:Catechols; D006873:Hydroquinones; C009760:mequinol; C015047:p-tert-butyl catechol; C031927:hydroquinone