Overexpression of L7/L12 protein with mutations in its flexible region.
Using restriction enzymes and polymerase chain reaction, three mutated forms of L7/L12 proteins with deleted 38-46, 44-52 and 38-52 residues were obtained. These mutant proteins were isolated in a preparative scale and were shown to bind to ribosomes and to affect ribosomal function.
Gudkov
A T
AT
Institute of Protein Research, Academy of Sciences of the USSR, Moscow Region.
Bubunenko
M G
MG
Gryaznova
O I
OI
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D002872:Chromosome Deletion; D004247:DNA / Q000235:genetics; D004926:Escherichia coli; D015870:Gene Expression; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000235:genetics; D012270:Ribosomes / Q000502:physiology
D001426:Bacterial Proteins; D012269:Ribosomal Proteins; C016700:ribosomal protein L7-L12; D004247:DNA