Collisional-activation tandem mass spectrometry of sodium adduct ions of methylated oligosaccharides: sequence analysis and discrimination between alpha-NeuAc-(2----3) and alpha-NeuAc-(2----6) linkages.
Collision-activated dissociation (c.a.d.) of sodium adducts of molecular ion species have been carried out on methylated beta-D-Galp-(1----4)-beta-D-GlcpNAc- (1----3)-beta-D-Galp-(1----4)-D-Glcp (1), beta-D-Galp-(1----3)-beta-D-GlcpNAc-(1----3)-beta-D-Galp-(1----4)-D-Glcp (2), alpha-D-NeuAc-(2----3)-beta-D-Galp-(1----3)-beta-D-GlcpNAc-(1----3)-beta -D-Galp - (1----4)-D-Glcp (3), alpha-D-NeuAc-(2----6)-beta-D-Galp-(1----4)-beta-D-GlcpNAc-(1----3)-beta -D-Galp - (1----4)-D-Glcp (4), and alpha-D-NeuAc-(2----6)-beta-D-Galp-(1----4)-beta-D-GlcpNAc-(1----2)-alph a-D- Manp-(1----3)-beta-D-Manp-(1----4)-D-GlcpNAc (5). The numerous daughter ions reflect the sequences, clearly differentiate (1----3) and (1----4) linkages, and discriminate between alpha-NeuAc-(2----3) and alpha-NeuAc-(2----6) linkages.
Lemoine
J
J
Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille Flandres-Artois (Unité Mixte de Recherche du C.N.R.S. No. 111), Villeneuve d'Ascq, France.
Strecker
G
G
Leroy
Y
Y
Fournet
B
B
Ricart
G
G
D002240:Carbohydrate Sequence; D007536:Isomerism; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D012794:Sialic Acids / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D009844:Oligosaccharides; D012794:Sialic Acids