[The resistance behavior of yeast isolates from defined patient groups to 5-fluorocytosine].
Susceptibility and development of resistance to 5-FC in Candida strains isolated from 4 defined groups of probands was investigated. 5-FC-susceptibility was determined in a microdilution assay in yeast nitrogen base after 24 h incubation at 37 degrees C. The range investigated ranked between 5-FC-concentrations from 0.015-16 microgram ml-1. Isolates with an MIC of greater than or equal to 16 micrograms ml-1 were regarded as 5-FC-resistant. In total 336 Candida isolates were investigated; 21 of them (= 6.3%) were found to be 5-FC-resistant. The Candida isolates were rather different with respect of their origin: 57 vaginal isolates from non-risk patients from Southern Germany comprised 5.3% 5-FC-resistant strains. 160 isolates from the urine of longtime-intensive care patients of total Germany were 5-FC-resistant with 6.3%. Of 74 isolates of different localization from intensive care patients of the University Clinics in Freiburg 10.8% were 5-FC-resistant. Among 45 isolates from the oral cavity from HIV-positive patients of the Frankfurt region no 5-FC-resistant strain was found. The epidemiology of 5-FC-resistance is mainly based on the percentage of non-albicans isolates of the proband groups (C. tropicalis, C. krusei and others), and is less based on the frequency of C. albicans serotype B isolates. In sequential observations with individual intensive care patients no increase of 5-FC-resistance in their Candida isolates could be observed with longer periods of hospitalization.
Weber
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S
Sektion Mykologie, Universität Freiburg.
Polak-Wyss
A
A
D002175:Candida / Q000187:drug effects*; D002177:Candidiasis / Q000382:microbiology* / Q000652:urine; D002180:Candidiasis, Oral / Q000382:microbiology*; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology*; D004352:Drug Resistance, Microbial; D005260:Female; D005437:Flucytosine / Q000494:pharmacology*; D015658:HIV Infections / Q000150:complications; D006801:Humans; D008297:Male
D005437:Flucytosine
Das Resistenzverhalten von Sprosspilzisolaten aus definierten Probandengruppen gegenüber 5-Fluorcytosin.