Rapid desalting of ammonium sulfate solutions by hydrophobic interaction chromatography.
Open-air hydrophobic interaction chromatography with alkyl carbon functional groups coupled to agarose beads has been used to desalt large volumes of ammonium sulfate-fractionated bacterial cell lysates. The protein of interest can be simultaneously desalted and concentrated in less than 4 h, and the yield is significantly better than that obtained by the standard technique of precipitation, centrifugation, and dialysis.
O'Hern
P A
PA
Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208.
Goldberg
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E
D000645:Ammonium Sulfate; D005591:Chemical Fractionation; D002846:Chromatography, Affinity / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification
D007527:Isoenzymes; D011506:Proteins; D011994:Recombinant Proteins; D007770:L-Lactate Dehydrogenase; D000645:Ammonium Sulfate