Autoradiographic study of 3H-uridine incorporation into nucleoli and DNA/3H-5S DNA in situ hybridization in root nodules and uninfected Lupinus luteus L. tissues.
The autoradiographic method was used to compare the 3H-uridine incorporation and the number of hybridization sites with 3H-5S DNA in yellow lupin root apical meristem, root hair of uninfected roots as well as in root nodule cortex and bacteriod-containing tissue. It has been shown that the number of hybridization sites is proportional to the ploidy level, but not to rRNA synthesis, which is most intense in root apical meristem and young bacteriod-containing cells.
Olszewska
M J
MJ
Department of Plant Cytology and Cytochemistry, University of Lódź, Poland.
Kononowicz
A K
AK
Madrzak
C J
CJ
Legocki
A B
AB
D001345:Autoradiography; D002466:Cell Nucleolus / Q000378:metabolism*; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D015342:DNA Probes / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D015138:RNA, Nuclear / Q000096:biosynthesis; D012341:RNA, Ribosomal, 5S / Q000096:biosynthesis*; D014316:Tritium; D014529:Uridine / Q000378:metabolism*
D015342:DNA Probes; D012333:RNA, Messenger; D015138:RNA, Nuclear; D012341:RNA, Ribosomal, 5S; D014316:Tritium; D004247:DNA; D014529:Uridine