Influence of subepithelial deposits on permeability of the glomerular capillary wall in serum sickness nephritis in the rat.
Serum sickness nephritis was induced in male Fisher rats by immunization with egg albumin (EA). Correlations of subepithelial deposits (SD) with size and charge barriers of the glomerular filter were investigated using native (NF) and cationized (CF) ferritin as tracer probes. In proteinuric animals large numbers of NF molecules perfused from the abdominal aorta were observed to cross the glomerular basement membrane (GBM) and enter SD. The concentration of NF molecules was higher in GBM segments with SD than in GBM segments without SD, and the concentration of these molecules was higher within SD than in the intervening GBM. In contrast, CF clusters were fewer in number in the lamina rara externa (LRE) of GBM segments with SD than in the GBM segments without SD. CF particles could not be observed within SD, even in the areas of podocyte detachment. It is suggested that permeability in GBM segments with SD increases and that the development of proteinuria in this model can be attributed to alterations in both charge- and size-selective barriers to glomerular filtration.
Duan
H J
HJ
Department of Pathology, Shinshu University School of Medicine, Matsumoto, Japan.
Nakazawa
K
K
Ishigame
H
H
Yamaguchi
N
N
Itoh
N
N
Shigematsu
H
H
D000818:Animals; D002199:Capillary Permeability; D004848:Epithelium / Q000737:chemistry / Q000503:physiopathology / Q000648:ultrastructure; D005293:Ferritins / Q000737:chemistry; D005920:Glomerular Mesangium / Q000098:blood supply* / Q000737:chemistry / Q000648:ultrastructure; D005921:Glomerulonephritis / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D011507:Proteinuria / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D011916:Rats, Inbred F344; D012713:Serum Sickness / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology
D005293:Ferritins