Method for the biological monitoring of hexahydrophthalic anhydride by the determination of hexahydrophthalic acid in urine using gas chromatography and selected-ion monitoring.
A method for the determination of hexahydrophthalic acid, a metabolite of hexahydrophthalic anhydride, in human urine has been developed. The urine was worked-up by liquid-solid extraction, esterified with boron trifluoride-methanol, and analysed by capillary gas chromatography and selected-ion monitoring. Hexadeuterium-labelled hexahydrophthalic acid was used as the internal standard. The precision was 4% at 0.7 microgram/ml and 5% at 0.07 microgram/ml. The recovery of the acid for the overall method was 101% at 0.07 micrograms/ml of urine (with a coefficient of variation of 4%) and 95% at 0.7 microgram/ml (coefficient of variation 2%). The limit of detection was 20 ng/ml urine.
Jönsson
B
B
Department of Occupational and Environmental Medicine, University Hospital, Lund, Sweden.
Skarping
G
G
D002849:Chromatography, Gas; D003509:Cyclohexanecarboxylic Acids / Q000652:urine*; D004853:Epoxy Resins / Q000378:metabolism* / Q000633:toxicity; D006801:Humans; D006868:Hydrolysis; D013058:Mass Spectrometry; D016273:Occupational Exposure; D010796:Phthalic Anhydrides / Q000378:metabolism* / Q000633:toxicity; D012015:Reference Standards
D003509:Cyclohexanecarboxylic Acids; D004853:Epoxy Resins; D010796:Phthalic Anhydrides; C074866:hexahydrophthalic acid; C005752:araldite