A 220 kDa coelomocyte aggregating factor involved in Holothuria polii cellular clotting.
Agglutinating molecules are released by Holothuria polii coelomocytes. In our in vitro system we observe that release depends on the number of coelomocytes but it seems not to be time- and temperature-dependent. The factor responsible for agglutination was isolated from an Edds isotonic solution coelomocyte suspension medium and purified by ammonium sulfate precipitation, gel filtration and ion exchange chromatography; it had a molecular mass of about 220 kDa on an sodium dodecyl sulfate polyacrylamide gel. The purified factor agglutinates sea cucumber coelomocytes suggesting that it could be involved in the first phase of clotting events.
Canicattí
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Department of Biology, University of Lecce, Italy.
Rizzo
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D000371:Agglutination / Q000502:physiology*; D000373:Agglutinins / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002469:Cell Separation; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D008970:Molecular Weight; D012616:Sea Cucumbers / Q000737:chemistry* / Q000166:cytology
D000373:Agglutinins; D002118:Calcium