Automated kinetic assay of beta-galactosidase activity.
An automated kinetic assay for beta-galactosidase activity in Escherichia coli was developed to permit the measurement of many independent samples simultaneously. Bacteria are grown, lysed from without (by adsorption of a high multiplicity of bacteriophage T4) and assayed in microtiter plates with 96 wells. Absorbance data are collected and analyzed by computer. The growth and lysis procedure, apparatus and software used in this assay can be used for other spectrophotometric enzyme assays.
Arvidson
D N
DN
California Institute of Biological Research, La Jolla 92037.
Youderian
P
P
Schneider
T D
TD
Stormo
G D
GD
D001331:Automation; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D013056:Spectrophotometry, Ultraviolet; D001616:beta-Galactosidase / Q000032:analysis*
D001616:beta-Galactosidase