Correlation between aminoglycoside resistance profiles and DNA hybridization of clinical isolates.
DNA hybridization data and aminoglycoside resistance profiles (AGRPs) were determined for 4,088 clinical isolates from three studies (United States, Belgium, and Argentina). The correlation between susceptibility profiles and hybridization results was determined with nine DNA probes. For each of the seven aminoglycoside resistance profiles which we were able to test, the data suggested at least two distinct genes could encode enzymes which lead to identical resistance profiles. Furthermore, the DNA hybridization data showed that individual strains carried up to six unique aminoglycoside resistance genes. DNA hybridization revealed interesting differences in the frequencies of these genes by organism and by country.
Shaw
K J
KJ
Schering-Plough Research, Bloomfield, New Jersey 07003.
Hare
R S
RS
Sabatelli
F J
FJ
Rizzo
M
M
Cramer
C A
CA
Naples
L
L
Kocsi
S
S
Munayyer
H
H
Mann
P
P
Miller
G H
GH
D000150:Acinetobacter / Q000187:drug effects / Q000235:genetics; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000235:genetics*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D004352:Drug Resistance, Microbial / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000235:genetics; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D015151:Immunoblotting; D008826:Microbial Sensitivity Tests; D009693:Nucleic Acid Hybridization; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics; D012705:Serratia / Q000187:drug effects / Q000235:genetics
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D015342:DNA Probes; D004269:DNA, Bacterial