A two-step purification procedure for sheep liver 6-phosphogluconate dehydrogenase.
A two-step procedure for the purification of 6-phosphogluconate dehydrogenase (EC 1.1.1.44; 6-PGDH) from sheep liver is described. The enzyme is directly bound to cellulose phosphate by batch extraction and eluted with a linear salt gradient. Purification is completed by affinity chromatography using NADP(+)-agarose. The result is 6-PGDH of high purity, greatly increased yield, and the highest specific activity yet achieved, with a significant reduction in the purification time.
Somers
D O
DO
Laboratory of Molecular Biophysics, University of Oxford, United Kingdom.
Hajdu
J
J
Adams
M J
MJ
D000818:Animals; D002482:Cellulose / Q000031:analogs & derivatives; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003956:Dialysis; D008099:Liver / Q000201:enzymology*; D010734:Phosphogluconate Dehydrogenase / Q000302:isolation & purification*; D012756:Sheep
D002482:Cellulose; C008881:phosphocellulose; D010734:Phosphogluconate Dehydrogenase