[Transitions of the left poly-L-proline II helix-right alpha helix type in the regions of myosin subfragment S2 of cross-striated muscle as a possible conformational basis for a mechanical-chemical act].
Local conformational states of fibrous fragments of myosin molecules from striated muscle have been studied. Analysis of the amino acid sequences of the rat embryonic skeletal muscle myosin heavy chain and the nematode myosin heavy chain have been performed with the aim to estimate the influence of electrostatic interactions on secondary structure stability of these fragments. The heterogeneity of stability of alpha-helical conformation along the fibrous fragment have been found on the basis of estimation of interaction between side group charges and between side group charges and main chain charges. Periodically located short sections have been found in the N-terminal half of the myosin rod where clusters of Asp and Glu destabilize alpha-helical structure being ionized. Changes of the distribution of charges near the latter sections bring about conformational transitions from left-handed polyproline II helix to right alpha-helix or vice versa. The new scheme of orientation of fibrous part of the cross-bridge in relation to the thick filament for various stages of muscle contraction process suggests asynchronous character of transitions in the different sites. It may be proposed that existence of alteration left- and right-helical fragments in N-terminal half of fibrous part of heavy myosin chain determines zigzag form of this part of myosin molecule in resting muscle. A model of the cross-bridge movement in the course of ATP hydrolysis has been suggested.
Pirtskhalava
M K
MK
Esipova
N G
NG
Tumanian
V G
VG
Zaalishvili
M M
MM
D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism*; D015879:Myosin Subfragments / Q000378:metabolism*; D009348:Nematoda; D010455:Peptides / Q000378:metabolism*; D011487:Protein Conformation; D051381:Rats
D015879:Myosin Subfragments; D010455:Peptides; C011083:polyproline; D000255:Adenosine Triphosphate
Perekhody tipa levaia spiral' poli-L-prolin II-pravaia alpha-spiral' v uchastkakh subfragmenta S2 miozina poperechnopolosatoĭ myshtsy kak vozmozhnaia konformatsionnaia osnova mekhano-khimicheskogo akta.