Preparation and characterization of monoclonal antibodies against recombinant human tumor necrosis factor alpha.
Tumor necrosis factor (TNF alpha) plays an important role in cytotoxicity and inhibition of tumor cells. Further studies on the structure, function and clinical application of TNF alpha will be useful. Nine clones of hybridoma secreting monoclonal antibodies against recombinant human tumor necrosis factor alpha (rHTNF alpha) were obtained by using cell fusion technology. None of the monoclonal antibodies cross-reacted with rIL-1, rIL-2, rIFN gamma, rIFN alpha and E. coli lysates. Western blot demonstrated that they specifically recognized the rHTNF antigen of M. W. 17000 daltons. Some of the antibodies can also recognize native rHTNF. These antibodies neutralized the cytotoxicity of rHTNF alpha to different extents. They will be utilized as immunoaffinity column to purify rHTNF alpha from recombinant E. coli lysates.
Li
Y
Y
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing.
Zhao
W W
WW
Shen
B F
BF
Sun
Y X
YX
Wang
W X
WX
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011994:Recombinant Proteins / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*
D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha