Regulation of the expression of mitogen-regulated protein (MRP; proliferin) and cathepsin L in cultured cells and in the murine placenta.
The genes encoding mitogen-regulated protein (MRP; also called proliferin; PLF) and procathepsin L (CL; also called major excreted protein; MEP) are expressed to high levels in the mouse placenta. Although they are both regulated by epidermal growth factor (EGF) and fibroblast growth factor (FGF) in 3T3 cells, expression of these genes is differently regulated with growth state. The expression patterns of MRP and CL as a function of murine development are also different. Basal and growth factor-stimulated levels of MRP expression are much higher in growing than in quiescent 3T3 cells, whereas CL levels are similar. These changes in gene expression in cultured quiescent cells parallel the changes in MRP and CL expression observed in the late-gestational quiescent placenta. These results suggest growth factors may regulate the expression of these genes, but other influences also regulate the expression of MRP and CL in vivo.
Nilsen-Hamilton
M
M
Department of Biochemistry and Biophysics, Iowa State University, Ames 50011.
Jang
Y J
YJ
Delgado
M
M
Shim
J K
JK
Bruns
K
K
Chiang
C P
CP
Fang
Y
Y
Parfett
C L
CL
Denhardt
D T
DT
Hamilton
R T
RT
D016475:3T3 Cells; D000818:Animals; D056668:Cathepsin L; D002403:Cathepsins / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D005786:Gene Expression Regulation; D005865:Gestational Age; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006133:Growth Substances / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D010920:Placenta / Q000378:metabolism*; D010929:Placentation; D011388:Prolactin; D012333:RNA, Messenger / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D016211:Transforming Growth Factor alpha / Q000494:pharmacology
D006023:Glycoproteins; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; C000634106:Prl2c2 protein, mouse; D012333:RNA, Messenger; D016211:Transforming Growth Factor alpha; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D011388:Prolactin; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; C534281:CTSL protein, human; D056668:Cathepsin L; C534278:Ctsl protein, mouse; D013755:Tetradecanoylphorbol Acetate