Location and quantitation of the sites of O-acetylation on the capsular polysaccharide from Streptococcus pneumoniae type 9V by 1H-n.m.r. spectroscopy: comparison with type 9A.
The 1H-n.m.r. spectra of the Streptococcus pneumoniae type 9V (S68 in American nomenclature) capsular polysaccharide (PS) and its O-deacetylated derivative [which is structurally identical to the S. pneumoniae type 9A (S33) PS] were assigned using COSY, relayed-COSY, and 2D-NOESY experiments. The positions of the OAc groups in the alpha-GlcA, beta-ManNAc, and alpha-Glc residues of the native 9V PS were established using 2D-n.m.r. and chemical shift arguments, and the relative proportions of different O-acetylated species were estimated by integration of well-resolved 1H-n.m.r. signals. The locations of the OAc substituents differ from those previously reported. [formula: see text].
Rutherford
T J
TJ
Chemistry Division, National Institute for Biological Standards & Control, Hertfordshire, Great Britain.
Jones
C
C
Davies
D B
DB
Elliott
A C
AC
D000107:Acetylation; D016667:Bacterial Capsules / Q000737:chemistry*; D002240:Carbohydrate Sequence; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006595:Hexosamines / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D013296:Streptococcus pneumoniae / Q000737:chemistry*
D006595:Hexosamines; D011135:Polysaccharides, Bacterial; C008322:mannosamine; D005947:Glucose; D005690:Galactose