Quantification of experimental pulmonary metastases by image analyzer.
Image analysis techniques for quantifying experimental pulmonary metastases were developed using Lewis lung carcinoma (3LL) and B16 melanoma. Binary images of the metastatic colonies were differentiated by staining the lung parenchyma. Thus, it became possible for the image analyzer to determine automatically the size and number of the metastatic foci. Image analysis was used successfully to evaluate the inhibitory effect of adriamycin on spontaneous metastases of 3LL. Although the inhibitory effect was not detected by the usual counting method, a significant reduction of the area of metastatic foci was detected by image analysis.
Iwahana
M
M
Exploratory Research Laboratories I, Daiichi Pharmaceutical Co., Ltd., Tokyo Japan.
Tohgo
A
A
Tanaka
N G
NG
Osada
Y
Y
D000818:Animals; D002277:Carcinoma / Q000188:drug therapy / Q000473:pathology / Q000556:secondary; D004317:Doxorubicin / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D008175:Lung Neoplasms / Q000188:drug therapy / Q000473:pathology* / Q000556:secondary; D008297:Male; D008545:Melanoma / Q000473:pathology / Q000556:secondary; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis / Q000473:pathology*; D009374:Neoplasms, Experimental / Q000188:drug therapy / Q000473:pathology*; D014407:Tumor Cells, Cultured
D004317:Doxorubicin