Neutralization of myotoxic activity of Bothrops venoms by antisera to purified myotoxins and to crude venoms.
A phospholipase myotoxin (MOO-1) and a non-phospholipase myotoxin (JSU-5) were studied for their antigenic cross-reactivity and neutralization by different antisera. Antisera against JSU-5 and MOO-1 reacted equally with both myotoxins in ELISA assays. The specificity of these antisera was also similar, recognizing the same 14,000-18,000 mol. wt components in the venoms of Bothrops jararacussu, Bothrops moojeni, Bothrops neuwiedi and Bothrops pradoi. Using creatine kinase assays, JSU-5 myotoxicity was completely neutralized by B. jararacussu antivenom or anti-JSU-5 antibodies and partially neutralized by B. moojeni antivenom or anti-MOO-1 antibodies. MOO-1 myotoxicity was completely neutralized by antisera against JSU-5 and MOO-1 and B. jararacussu antivenom, and only partially neutralized by B. moojeni antivenom. B. jararacussu venom induced high titres of antibodies against purified myotoxins. This antiserum completely inhibited the myotoxicity of the homologous venom and significantly reduced the myotoxicity of the remaining myotoxin-containing venoms. It is suggested that B. jararacussu venom is a good immunogen to induce antibodies against myotoxins present in the venoms of the different species of Bothrops.
Moura-da-Silva
A M
AM
Instituto Butantan, São Paulo, Brazil.
Cardoso
D F
DF
Tanizaki
M M
MM
Mota
I
I
D000818:Animals; D000918:Antibody Specificity; D000997:Antivenins / Q000627:therapeutic use*; D015153:Blotting, Western; D003402:Creatine Kinase / Q000097:blood; D003429:Cross Reactions; D003435:Crotalid Venoms / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008815:Mice, Inbred Strains; D009135:Muscular Diseases / Q000139:chemically induced / Q000628:therapy*; D009500:Neutralization Tests; D012911:Snakes / Q000276:immunology; D013045:Species Specificity
D000997:Antivenins; D003435:Crotalid Venoms; D003402:Creatine Kinase