title: Impact of surface modifications of Acanthocheilonema viteae microfilariae on cell adhesion.
abstract: Exposure of A. viteae microfilariae to various lectins reduced their capacity to react with the peritoneal exudate cells of the host, Mastomys natalensis. Sugars corresponding to these lectins with the exception of N-acetyl glucosamine, did not affect the adhesion per se. They however, protected the parasite against the adverse effect of lectins. Neuraminidase and chitinase also suppressed adhesion capacity of the microfilariae. Except sodium dodecylsulphate which enhanced cell attachment, other surfactants inhibited this reaction considerably. The results indicate that antibody dependent adhesion of the microfilariae with the macrophages involves surface moieties of the parasite, where N-acetylglucosamine acts as the principal sugar residue. Participation of -SH groups also is inferred from the observations that p-chloromercuribenzoate and dithiobis-(2-nitrobenzoic acid) inhibited cell attachment and dithiothreitol provided protection against these agents.
authors.affiliation: Division of Parasitology, Central Drug Research Institute, Lucknow, India.
authors.forename: D P
authors.initials: DP
authors.lastname: Singh
authors.forename: R K
authors.initials: RK
authors.lastname: Chatterjee
authors.forename: V M
authors.initials: VM
authors.lastname: Srivastava
mesh_terms: D000116:Acetylgalactosamine / Q000494:pharmacology; D000117:Acetylglucosamine / Q000494:pharmacology; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D004153:Dipetalonema / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D006601:Hexoses / Q000494:pharmacology; D006790:Host-Parasite Interactions / Q000187:drug effects; D006867:Hydrolases / Q000494:pharmacology; D037102:Lectins; D008842:Microfilariae / Q000187:drug effects*; D009115:Muridae; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology
chemical_list: D006601:Hexoses; D037102:Lectins; D013439:Sulfhydryl Reagents; D013501:Surface-Active Agents; D006867:Hydrolases; D000116:Acetylgalactosamine; D000117:Acetylglucosamine