Effect of alcohol on blood levels of branched-chain alpha-keto acids in male Wistar rats.
The effect of oral alcohol administration on branched-chain alpha-keto acids was studied in Wistar rats. Rats were divided into alcohol loading groups 1 (ethanol single daily dose: 2.5 g/kg body wt for 14 days) and 2 (ethanol dose: 5 g/kg body wt), and a pair-fed control group (isocaloric glucose and food equal to the amount taken by group 1 the previous day). Branched-chain alpha-keto acids, other alpha-keto acids and amino acids were analysed using high-performance liquid chromatography. Results showed a significant body weight increase in the alcohol loading groups. Concentrations of branched-chain amino acids and keto acids were significantly increased in the alcohol-treated groups. The blood levels of methionine, threonine, alanine and glutamic acid were also increased by alcohol administration. Blood levels of taurine and ornithine, however, showed a significant decrease in the alcohol-treated groups. These results show that alcohol increases the blood levels of branched-chain keto acids and branched-chain amino acids.
Dominicus
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DA
Department of Preventive Medicine, School of Medicine, University of the Ryukyus, Okinawa, Japan.
Todoriki
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H
Ariizumi
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M
Akamatsu
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T
D000428:Alcohol Drinking / Q000097:blood; D000437:Alcoholism / Q000097:blood*; D000597:Amino Acids, Branched-Chain / Q000097:blood*; D000818:Animals; D007651:Keto Acids / Q000097:blood*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000597:Amino Acids, Branched-Chain; D007651:Keto Acids