Solid-phase extraction of midazolam and two of its metabolites from plasma for high-performance liquid chromatographic analysis.
A rapid, sensitive and selective assay of midazolam and two of its metabolites in plasma, based on high-performance liquid chromatography, has been developed. The compounds are subjected to solid-phase extraction, using C18 cartridges (Bond-Elut). Recoveries are in excess of 90% for midazolam and its metabolites. The limit of quantitation of the assay is 50 ng/ml of plasma for each compound.
Sautou
V
V
Laboratoire de Pharmacologie et Pharmacie Clinique et Biotechniques, U.F.R. Pharmacie, Clermont-Ferrand, France.
Chopineau
J
J
Terrisse
M P
MP
Bastide
P
P
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008874:Midazolam / Q000031:analogs & derivatives* / Q000097:blood*
C040081:1-hydroxymethylmidazolam; D008874:Midazolam