Long term maintenance of Toxoplasma gondii (Rh strain) in Vero cell line and use of harvested antigens for immunodiagnosis.
Thirty in vitro serial passages of Toxoplasman gondii cultures in Vero cell line performed once in every five days had a mean increase in parasite count of 74.4 +/- 14.8 times from that of initial counts. Long term cultures in Vero cell line did not alter the virulence of the parasite. The good correlation (r = 0.99) between the IFA titer and ELISA OD values using the parasite antigens from in vitro sources indicates that long term maintenance of T. gondii in culture does not affect significantly the ability to recognize antibodies to surface and soluble antigens. The results also show that soluble antigens containing host cells can be directly used for immunodiagnostic purposes without purification. The in vitro maintenance of T. gondii is safer and cheaper when compared to the in vivo method.
Suresh
K
K
Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia.
Mak
J W
JW
Yong
H S
HS
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D051379:Mice; D012692:Serial Passage; D014122:Toxoplasma / Q000254:growth & development* / Q000276:immunology / Q000472:pathogenicity; D014124:Toxoplasmosis, Animal / Q000175:diagnosis*; D014709:Vero Cells; D014774:Virulence
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan