[Determination of ergosterol in Cordyceps sinensis and Cordyceps black-bone chicken capsules by HPLC].
A high performance liquid chromatographic method (HPLC) was developed for the determination of ergosterol in Cordyceps sinensis and Cordyceps black-bone chicken capsules. The sample was mixed with 0.5 ml of saturated KOH solution and 5 ml of ethanol, and refluxed for 1 h. The saponified mixture was diluted with 5 ml of 10% NACl solution and extracted 3 times with 5 ml portions of cyclohexane. The cyclohexane extracts were purified by partition column and concentrated by adsorption column and then analysed by HPLC. The other components of the Cordyceps black-bone chicken capsules have no interference to the determination of ergosterol. The proposed method is rapid, precise, accurate and sensitive. The whole process can be done in 2 h.
Li
Y H
YH
School of Pharmacy, Second Military Medical University, Shanghai.
Li
X L
XL
D001203:Ascomycota / Q000737:chemistry*; D002214:Capsules; D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004875:Ergosterol / Q000032:analysis*; D006999:Hypocreales / Q000737:chemistry*; D007915:Lepidoptera / Q000737:chemistry*
D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D004875:Ergosterol