[The effects and properties of sodium nucleinate as a pyrogen working-standard. 9. Pyrogen detection with epinephrine-skin-, dactinomycin- and LAL-tests. The suitability of sodium nucleinate as a pyrogen standard].
Sodium nucleinate (NN) as well as bacterial lipopolysaccharide (LPS) can be detected by epinephrine-skin, dactinomycin and LAL tests. In the quantitative determination of two pyrogen standards for rabbit tests, consisting of NN, a smaller value was found by LAL test for the standard of greatest pyrogenic effect than for that less pyrogenically effective in rabbits. A standard consisting of NN can be used for the pyrogen test in rabbits. But in the future, if necessary a standard consisting of endotoxin will be used, due to its better comparability of results obtained by LAL and rabbit tests.
Dressel
H
H
Institut für Arzneimittelwesen, Berlin.
D000818:Animals; D002417:Cattle; D003609:Dactinomycin; D004837:Epinephrine; D008070:Lipopolysaccharides; D051379:Mice; D009696:Nucleic Acids / Q000737:chemistry / Q000494:pharmacology*; D011749:Pyrogens / Q000737:chemistry / Q000494:pharmacology*; D012015:Reference Standards; D012867:Skin / Q000187:drug effects
D008070:Lipopolysaccharides; D009696:Nucleic Acids; D011749:Pyrogens; D003609:Dactinomycin; C013683:sodium nucleinate; D004837:Epinephrine
Uber Wirkungen und Eigenschaften des als Pyrogen-Arbeitsstandard verwendeten Natriumnucleinats. 9. Mitteilung: Pyrogennachweis mit Epinephrin-Haut-, Dactinomycin- und LAL-Test/Zur Eignung des Natriumnucleinats als Pyrogen-Standard.