[Analysis of the replication kinetics in primary culture of isolates of HIV-1 from infected patients in various stages of the disease].
The authors have studied the replicative kinetics and the induction of cytopathic effects of HIV-1 in primary co-cultures from infected subjects at various stages of the disease. Cultures from subjects with ARC or AIDS yielded HIV-1 replication more precociously and at higher levels compared to those from asymptomatic subjects; cytopathic effect "in vitro" were observed more frequently and earlier in cell cultures from ARC/AIDS subjects. Presented data indicate that the clinical and immunological deterioration during HIV-1 infection is related to viral replicative activity and suggest that the study of HIV-1 replicative kinetics in primary co-cultures may be helpful in predicting who will progress to AIDS.
Fiore
J R
JR
Clinica di Malattie Infettive, Università di Bari.
Angarano
G
G
Fico
C
C
Di Stefano
M
M
Monno
L
L
Pastore
G
G
D000386:AIDS-Related Complex / Q000382:microbiology; D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D015658:HIV Infections / Q000382:microbiology*; D015497:HIV-1 / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D013601:T-Lymphocytes / Q000382:microbiology; D014776:Virus Cultivation; D014779:Virus Replication
Analisi della cinetica replicativa in coltura primaria di isolati di HIV-1 da soggetti infetti in vario stadio di malattia.