Hormone responsive elements within the upstream sequences of the rabbit whey acidic protein (WAP) gene direct chloramphenicol acetyl transferase (CAT) reporter gene expression in transfected rabbit mammary cells.
Whey acidic protein gene transcription is induced in the mammary gland under the influence of lactogenic hormones: prolactin, insulin and cortisol. The rabbit WAP gene has already been isolated and sequenced in a previous work. In the present study, we have evaluated the role of the 5' flanking region of the rabbit WAP gene in the transcriptional regulation of the WAP gene by using a reporter CAT gene. Chimeric genes containing the upstream region of the WAP gene have been linked to the bacterial CAT gene and transfected into rabbit primary mammary cells. The results reported here show that two regions carrying important regulatory elements of the rabbit WAP gene are located between -6300 and -3000 bp, and between -3000 and -1800 bp upstream from the WAP transcription start point, respectively. The contribute to the high level of expression of the rabbit WAP gene in the mammary cell.
Devinoy
E
E
Unité de Différenciation Cellulaire, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
Maliénou-N'Gassa
R
R
Thépot
D
D
Puissant
C
C
Houdebine
L M
LM
D000818:Animals; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D006854:Hydrocortisone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007774:Lactation / Q000235:genetics; D008321:Mammary Glands, Animal / Q000166:cytology / Q000502:physiology*; D008894:Milk Proteins / Q000235:genetics*; D011388:Prolactin / Q000494:pharmacology; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics
D007328:Insulin; D008894:Milk Proteins; D011994:Recombinant Proteins; C024471:whey acidic proteins; D011388:Prolactin; D015500:Chloramphenicol O-Acetyltransferase; D001616:beta-Galactosidase; D006854:Hydrocortisone