title: Expression and characterization of recombinant second domain variants of human mucus proteinase inhibitor (MPI).
abstract: The second, carboxyterminal domain of human mucus proteinase inhibitor (MPI) represents a strong antagonist of trypsin, chymotrypsin and leucocyte elastase. To modulate the inhibitory specificity and chemical stability of this domain, mutants have been prepared by site-directed mutagenesis of a cDNA fragment encoding for the carboxyterminal half of the inhibitor, followed by expression in E. coli. Inhibition assays with the purified recombinant domains revealed the possibility to create variants for potential pharmaceutical use.
authors.affiliation: Institut für Biochemie, THD, Darmstadt, FRG.
authors.forename: B
authors.initials: B
authors.lastname: Meckelein
authors.forename: M
authors.initials: M
authors.lastname: Kemme
authors.forename: T
authors.initials: T
authors.lastname: Nikiforov
authors.forename: H
authors.initials: H
authors.lastname: Appelhans
authors.forename: H G
authors.initials: HG
authors.lastname: Gassen
mesh_terms: D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics; D015842:Serine Proteinase Inhibitors / Q000235:genetics* / Q000378:metabolism
chemical_list: D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D011994:Recombinant Proteins; D015842:Serine Proteinase Inhibitors