title: High-performance liquid chromatographic separation of the enantiomers of hydroxychloroquine and its major metabolites in biological fluids using an alpha 1-acid glycoprotein stationary phase.
abstract: An enantioselective two-stage off-line assay has been developed for the analysis of hydroxychloroquine and its three major metabolites in biological fluids. The first non-stereoselective stage of the assay (PRP-1 column) separates and quantitates parent drug and metabolites. Fractions containing hydroxychloroquine and each of the metabolites are collected manually, evaporated, reconstituted in mobile phase and re-injected onto an alpha 1-acid glycoprotein column to separate and determine proportions of individual enantiomers. Preliminary results from patients samples indicate that the disposition of hydroxychloroquine and its major metabolites is enantioselective. p6
authors.affiliation: Department of Pharmacy, University of Sydney, NSW, Australia.
authors.forename: A J
authors.initials: AJ
authors.lastname: McLachlan
authors.forename: S E
authors.initials: SE
authors.lastname: Tett
authors.forename: D J
authors.initials: DJ
authors.lastname: Cutler
mesh_terms: D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006886:Hydroxychloroquine / Q000378:metabolism*; D009961:Orosomucoid; D013237:Stereoisomerism
chemical_list: D009961:Orosomucoid; D006886:Hydroxychloroquine