Isolation of endothelial cells from human first trimester decidua using immunomagnetic beads.
A technique was developed for the isolation of purified endothelial cells from first trimester decidual tissue, using QB End/40-coated magnetic polystyrene beads (Dynabeads). QB End/40 is an endothelial-specific monoclonal antibody which reacts with the coagulation cascade co-factor, thrombomodulin. Small endothelial cell aggregates were released from first trimester decidua by the sequential use of collagenase and trypsin. This dissociation method yielded 15-20% endothelial cells which were further purified to greater than 90% homogeneity by rosetting with QB End/40-Dynabeads. Cultures of purified decidual endothelial cells provide a useful tool for investigating cell-cell interaction in the first trimester placental bed.
Drake
B L
BL
Department of Cellular and Genetic Pathology, University of Cambridge, UK.
Loke
Y W
YW
D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003656:Decidua / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology*; D005260:Female; D006801:Humans; D007158:Immunologic Techniques; D008280:Magnetics; D008863:Microspheres; D011137:Polystyrenes; D011247:Pregnancy
D011137:Polystyrenes