Comparison of high purity factor IX concentrates and a prothrombin complex concentrate in a canine model of thrombogenicity.
A non-stasis canine model of thrombogenicity has been used to evaluate batches of high purity factor IX concentrates from 4 manufacturers and a conventional prothrombin complex concentrate (PCC). Platelets, activated partial thromboplastin time (APTT), fibrinogen, fibrin(ogen) degradation products and fibrinopeptide A (FPA) were monitored before and after infusion of concentrate. Changes in FPA were found to be the most sensitive and reproducible indicator of thrombogenicity after infusion of batches of the PCC at doses of between 60 and 180 IU/kg, with a dose related delayed increase in FPA occurring. Total FPA generated after 100-120 IU/kg of 3 batches of PCC over the 3 h time course was 9-12 times that generated after albumin infusion. In contrast the amounts of FPA generated after 200 IU/kg of the 4 high purity factor IX products were in all cases similar to albumin infusion. It was noted that some batches of high purity concentrates had short NAPTTs indicating that current in vitro tests for potential thrombogenicity may be misleading in predicting the effects of these concentrates in vivo.
MacGregor
I R
IR
National Science Laboratory, Scottish National Blood Transfusion Service, Edinburgh, United Kingdom.
Ferguson
J M
JM
McLaughlin
L F
LF
Burnouf
T
T
Prowse
C V
CV
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D001779:Blood Coagulation Factors / Q000008:administration & dosage / Q000494:pharmacology*; D001780:Blood Coagulation Tests; D001792:Blood Platelets / Q000502:physiology; D004285:Dogs; D005164:Factor IX / Q000008:administration & dosage / Q000494:pharmacology*; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis*; D005340:Fibrinogen / Q000032:analysis; D005344:Fibrinopeptide A / Q000032:analysis; D007262:Infusions, Intravenous; D007700:Kinetics; D013997:Time Factors
D001779:Blood Coagulation Factors; D005338:Fibrin Fibrinogen Degradation Products; D005344:Fibrinopeptide A; C025667:prothrombin complex concentrates; D005164:Factor IX; D005340:Fibrinogen