Preclinical evaluation of 99m technetium-labeled DD-3B6/22 Fab' for thrombus detection.
Injection of 99mTc-labeled Fab' fragments of the anti-fibrin antibody DD-3B6/22, in the baboon, resulted in clear visualisation of both fresh and aged autologous thrombi by gamma scintigraphy. Whole body scintigraphy, pharmacokinetics and urine analysis showed rapid renal excretion of the conjugate with little accumulation of label in other organs. 99mTc-DD-3B6/22 Fab' appears a suitable candidate for further investigation as a radioimaging agent for thrombi in humans.
Walker
K Z
KZ
Centenary Institute of Cancer Medicine and Cell Biology, Sydney University, New South Wales, Australia.
Boniface
G R
GR
Phippard
A F
AF
Harewood
W
W
Bautovich
G J
GJ
Bundesen
P G
PG
D000818:Animals; D004353:Drug Evaluation, Preclinical; D005338:Fibrin Fibrinogen Degradation Products / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments; D008297:Male; D010215:Papio; D010690:Phlebography; D016719:Radioimmunodetection; D013667:Technetium / Q000493:pharmacokinetics; D013927:Thrombosis / Q000175:diagnosis* / Q000378:metabolism
D005338:Fibrin Fibrinogen Degradation Products; D007140:Immunoglobulin Fab Fragments; C036309:fibrin fragment D; D013667:Technetium