Measurement of translational accuracy in vivo: missense reporting using inactive enzyme mutants.
The measurement and potential technological significance of in vivo missense errors are briefly reviewed. A recently developed approach is described in which reporter enzyme activity is generated by mistranslation of a gene coding for an inactive mutant form of the enzyme. Initial results obtained using the alpha subunit of E coli tryptophan synthetase and bacterial luciferase are discussed, as well as the prospects for further development of this method.
Cornut
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B
Department of Chemical Engineering, University of Houston, TX 77204-4792.
Willson
R C
RC
D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D008156:Luciferases / Q000235:genetics*; D009154:Mutation; D014176:Protein Biosynthesis; D013307:Streptomycin / Q000494:pharmacology; D014367:Tryptophan Synthase / Q000235:genetics*
D008156:Luciferases; D014367:Tryptophan Synthase; D013307:Streptomycin