Induction of immune resistance against L1210 lymphatic leukemia in mice after lethal irradiation and reconstruction with fetal liver cells.
The immunohematopoietic potential of syngeneic fetal liver cells (SFLC) was examined and compared with syngeneic bone marrow cells (SBMC). SFLC generated about 3 times less 12th-day spleen colonies (CFU-S) than adult SBMC did. To test the SFLC ability for reconstitution of the immune system, mice were lethally total body irradiated (TBI) and transplanted i.v. with 3 x 10(7) SFLC or 1 x 10(7) SBMC. Thus, injected hematopoietic cells contained the same number of CFU-S. On days 28, 35, 42, and 49 after transplantation the mice were injected i.p. with 10(6) immunogenic L1210-Maf cells (L1210 leukemia cells treated in vitro with mafosfamide for inhibition of their growth in vivo) to test the ability for generation of immune response against L1210 leukemia. On day 56 the animals were challenged with 10(3) L1210 leukemia cells. Strong resistance against the leukemia was induced in TBI + SFLC and TBI + SBMC mice, suggesting that the SFLC similarly as SBMC are able to reconstitute immune system of the TBI host.
Skórski
T
T
Medical Centre of Postgraduate Education, Department of Cytophysiology, Warszawa, Poland.
Ratajczak
M Z
MZ
Kawalec
M
M
Kawiak
J
J
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D001853:Bone Marrow / Q000473:pathology; D002469:Cell Separation; D003131:Combined Modality Therapy; D003520:Cyclophosphamide / Q000031:analogs & derivatives / Q000627:therapeutic use; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000502:physiology; D007108:Immune Tolerance / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects*; D007113:Immunity, Innate / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects*; D007939:Leukemia L1210 / Q000188:drug therapy / Q000276:immunology* / Q000532:radiotherapy*; D007942:Leukemia, Experimental / Q000188:drug therapy / Q000276:immunology* / Q000532:radiotherapy*; D008099:Liver / Q000166:cytology* / Q000196:embryology* / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D014916:Whole-Body Irradiation
D000970:Antineoplastic Agents; C048341:mafosfamide; D003520:Cyclophosphamide