Adherence to experimental pellicle of rough-type lipopolysaccharides from subgingival plaque bacteria.
The adherence of lipopolysaccharides (LPSs) from periodontal disease-associated bacteria to saliva-coated hydroxyapatite (S-HA) and serum-coated HA beads was examined by chromogenic Limulus activity (toxicolor test). Phenol-water extracted LPS preparations from Bacteroides intermedius, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans, Eikenella corrodens, and rough-type LPS from Escherichia coli adhered to S-HA and serum-coated beads and agglutinated human erythrocytes. The adhered LPSs to S-HA and serum-coated HA beads were not removed by vigorous washing with distilled water. LPSs from Bacteroides (Porphyromonas) gingivalis strains and wild-type E. coli did not adhere to S-HA, serum-coated HA beads or show hemagglutinating activity. SDS-PAGE patterns stained with silver stain showed that LPSs adhered to S-HA, and serum-coated HA beads and erythrocytes possessed a distinct fast-migrating band similar to rough-type LPS. B. gingivalis LPSs possessed slow-migrating and repeating ladder bands similar to wild-type LPS.
Okuda
K
K
Department of Microbiology, Tokyo Dental College, Chiba City.
Kato
T
T
Ishihara
K
K
Naito
Y
Y
D001422:Bacterial Adhesion; D001769:Blood; D044622:Dental Pellicle; D003773:Dental Plaque / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D006090:Gram-Negative Bacteria / Q000502:physiology*; D006384:Hemagglutination; D006882:Hydroxyapatites; D008070:Lipopolysaccharides / Q000737:chemistry / Q000502:physiology*; D012463:Saliva / Q000382:microbiology*; D013499:Surface Properties
D006882:Hydroxyapatites; D008070:Lipopolysaccharides