Mammalian FMRF-NH2-like peptide in rat pituitary: decrease by osmotic stimulus.
Previous studies with the Brattleboro rat suggested a possible interaction at the pituitary level between AVP and the neuropeptide, F-8-F-NH2. In order to test this hypothesis, we studied the effect of various osmotic stimuli on neurohypophyseal F-8-F-NH2. In rats drinking 2% NaCl solution for two days, neural lobe AVP and F-8-F-NH2 levels were equally reduced by 87%. After maximal depletion, pituitary levels of F-8-F-NH2 and AVP rebounded in parallel when normal drinking water was reintroduced. Pituitary stalk transection depleted neurohypophyseal F-8-F-NH2. The results of this study suggest that neurohypophyseal F-8-F-NH2 originates from the hypothalamus and, furthermore, is coreleased along with AVP in response to hyperosmotic stimuli.
Majane
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Laboratory of Biochemical Genetics, NIMH Neuroscience Center, St. Elizabeths, Washington, DC 20032.
Yang
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D000595:Amino Acid Sequence; D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D007700:Kinetics; D008297:Male; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry / Q000378:metabolism*; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D009997:Osmotic Pressure; D010904:Pituitary Gland, Posterior / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012462:Saline Solution, Hypertonic
D009479:Neuropeptides; D009842:Oligopeptides; D012462:Saline Solution, Hypertonic; D001127:Arginine Vasopressin; C053651:phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide