Inter-laboratory ring trial of in vitro DNA repair tests using rat hepatocytes: further testing and conclusive remarks.
The results of the extension of a collaborative study for the detection of chemical-induced DNA damage in rat hepatocytes in vitro are presented in this report. Three coded compounds, i.e. 1,4-butanediol dimethanesulphonate, hydrazine sulphate and sodium dichromate, were tested for DNA repair synthesis by seven different laboratories, either using autoradiographic procedures or the liquid scintillation counting technique. Inter-laboratory standardization was intentionally not requested in order to investigate the validity of each study design under routine conditions. 1,4-Butanediol dimethanesulphonate was clearly positive in most laboratories; sodium dichromate was generally positive, while the results on hydrazine sulphate were contradictory.
Puri
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EC
Dr Karl Thomae GmbH, Ciba-Geigy Ltd, Basle, Switzerland.
Baumeister
M
M
Fautz
R
R
Hechenberger
C M
CM
Hertner
T
T
Meli
C
C
Miltenburger
H G
HG
Schmid
B
B
Schwegler
R
R
Seeberg
A M
AM
D000818:Animals; D001345:Autoradiography; D002066:Busulfan / Q000633:toxicity; D002478:Cells, Cultured; D002840:Chromates / Q000633:toxicity; D004249:DNA Damage; D004260:DNA Repair; D006834:Hydrazines / Q000633:toxicity; D007753:Laboratories / Q000592:standards; D008099:Liver / Q000166:cytology; D009152:Mutagenicity Tests / Q000379:methods / Q000592:standards*; D051381:Rats; D012588:Scintillation Counting
D002840:Chromates; D006834:Hydrazines; C029424:hydrazine; C016104:sodium bichromate; D002066:Busulfan