Preparation of enteric-coated microspheres of Mycoplasma hyopneumoniae vaccine with cellulose acetate phthalate: (II). Effect of temperature and pH on the stability and release behaviour of microspheres.
The in vitro stability (temperature and pH) and dissolution study (pH 7.4 phosphate buffer solution and pH changed medium) of the enteric-coated microspheres containing Mycoplasma hyopneumoniae vaccine (MHV) were examined. The MHV microspheres were thermally more stable than the unencapsulated MHV. More than 90% of antigenicity was retained in the MHV microspheres for 3 weeks when stored at 4 degrees C. The MHV microspheres in pH 1.2 and pH 3.0 medium were more stable than the unencapsulated MHV. The MHV enteric-coated microspheres exhibited an excellent enteric function to prevent pH-related inactivation. The influence of particle size, CAP concentration and span 80 concentration on the MHV released from microspheres was also determined. The smaller the particle size, the higher the dissolution rate due to the larger surface area of the smaller particle. The higher the concentration of span 80 used, the more the greater the amount of MHV released. This was attributed to the more porous structure of microspheres prepared by the higher concentration of span 80. By increasing the CAP concentration, however, the release rate of MHV was decreased due to the larger amount of CAP and the more compact structure of microspheres.
Lin
S Y
SY
Department of Medical Research, Veterans General Hospital-Taipei, Republic of China.
Tzan
Y L
YL
Weng
C N
CN
Lee
C J
CJ
D000818:Animals; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D009174:Mycoplasma / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology; D014612:Vaccines / Q000008:administration & dosage*
D003692:Delayed-Action Preparations; D014612:Vaccines; D002482:Cellulose; C011708:cellulose acetate phthalate