Characterization of anti-Leb antibody purified from serum of immunized rabbits.
An anti-Le(b) antibody was produced in sera of rabbits by immunization with human saliva from blood group O Le(a-b+) secretor and purified by sequential use of silica beads immobilized with H type 1, Le(a) and Le(b). The purified antibody agglutinated only Le(a-b+) red cells irrespective of their ABO blood type. Hemagglutination reaction with the antibody of blood group O Le(a-b+) red cells was inhibited not only by saliva samples from blood group Le(a-b+) secretors and Synsorb beads immobilized with Le(b) hapten, but also weakly by Synsorb immobilized with Y and H type 2 haptens.
Hosomi
O
O
Department of Legal Medicine, Gunma University School of Medicine.
Yazawa
S
S
Takeya
A
A
D000818:Animals; D001788:Blood Grouping and Crossmatching; D002240:Carbohydrate Sequence; D006241:Haptens; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006801:Humans; D007114:Immunization; D007518:Isoantibodies / Q000097:blood*; D007983:Lewis Blood Group Antigens / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000276:immunology; D011817:Rabbits
D006241:Haptens; D007518:Isoantibodies; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides