title: Characterization of an Agrobacterium tumefaciens lectin.
abstract: An Agrobacterium tumefaciens suspension induces a strong agglutination of aldehyde-fixed pig erythrocytes at pH 5.0. The agglutination is inhibited by some polysaccharides, such as fucoidin, and also when the pH is raised to 7.0. Lectins (sugar-binding proteins) associated with the bacterial cell wall of A. tumefaciens strain 84.5 were directly evidenced by spectrofluorimetry using fluoresceinylated neoglycoproteins. The specific binding of the fluorescein-labelled neoglycoprotein bearing alpha-L-fucoside residues was also optimal at pH 5.0. A lectin was purified by affinity chromatography on agarose substituted with alpha-L-fucopyranoside. Furthermore, the haemagglutination activity of this lectin was inhibited by polysaccharides isolated from poplar leaves.
authors.affiliation: Département de Biochimie des Glycoconjugués et Lectines Endogènes, Centre de Biophysique Moléculaire, C.N.R.S., Orléans, France.
authors.forename: C
authors.initials: C
authors.lastname: Depierreux
authors.forename: H C
authors.initials: HC
authors.lastname: Kang
authors.forename: B
authors.initials: B
authors.lastname: Guérin
authors.forename: M
authors.initials: M
authors.lastname: Monsigny
authors.forename: F
authors.initials: F
authors.lastname: Delmotte
mesh_terms: D016960:Agrobacterium tumefaciens / Q000276:immunology*; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000378:metabolism; D006384:Hemagglutination / Q000187:drug effects; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007525:Isoelectric Focusing; D037102:Lectins / Q000302:isolation & purification*; D008970:Molecular Weight; D010936:Plant Extracts; D037121:Plant Lectins; D011134:Polysaccharides / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013552:Swine; D014197:Trees
chemical_list: D002241:Carbohydrates; D006023:Glycoproteins; D037102:Lectins; D010936:Plant Extracts; D037121:Plant Lectins; D011134:Polysaccharides