Selective and quantitative isolation and determination of apomorphine in human plasma.
A simple extraction system for the selective and quantitative isolation of apomorphine from human plasma is described. Apomorphine and N-n-propylnorapomorphine were isolated by complex formation between a borate group and the diol group of the apomorphines in an alkaline medium, this in combination with ion-pair formation. The reproducibility and linearity of this extraction method combined with high-performance liquid chromatography with electrochemical detection is excellent. The absolute mean recovery of apomorphine was 100%, the recovery of N-n-propylnorapomorphine was 98%. The detection limit of apomorphine in human plasma in the described system is approximately 0.5 ng/ml.
Essink
A W
AW
Department of Clinical Pharmacy, Medisch Spectrum Twente, Enschede, Netherlands.
Lohuis
C P
CP
Klein Elhorst
J T
JT
Rutten
W J
WJ
D001058:Apomorphine / Q000031:analogs & derivatives / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D015203:Reproducibility of Results
C009074:N-n-propylnorapomorphine; D001058:Apomorphine