title: Amino acid sequence of the 8-kDa protein in photosystem I reaction center complex from a thermophilic cyanobacterium, Synechococcus elongatus.
abstract: The 8-kDa protein in Photosystem I (PS I) reaction center complex was isolated from a thermophilic cyanobacterium, Synechococcus elongatus, by SDS-polyacrylamide gel electrophoresis using TRIS-Tricine buffer system. The complete amino acid sequence of the protein was determined. The 8-kDa protein consisted of 73 amino acid residues giving a calculated molecular weight of 7,472. No significant sequence homology were observed with the known other small subunits in PS I reaction center complex, except for the 6.5-kDa protein in PS I from another thermophilic cyanobacterium, S. vulcanus. The 8-kDa protein was characteristically rich in hydrophobic amino acid residues, especially the content of leucine. These suggest that the 8-kDa subunit is an intrinsic structure component in PS I core complex for stabilization of the reaction center.
authors.affiliation: Research Institute for Biosciences, Science University of Tokyo, Japan.
authors.forename: C S
authors.initials: CS
authors.lastname: Jone
authors.forename: N
authors.initials: N
authors.lastname: Kotani
authors.forename: K
authors.initials: K
authors.lastname: Aso
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.forename: I
authors.initials: I
authors.lastname: Enami
authors.forename: K
authors.initials: K
authors.lastname: Kondo
authors.forename: A
authors.initials: A
authors.lastname: Tsugita
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000458:Cyanobacteria / Q000737:chemistry*; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D045331:Photosystem I Protein Complex; D016415:Sequence Alignment
chemical_list: D000596:Amino Acids; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex