title: Characterisation of polysaccharides by in-source pyrolysis positive- and negative-ion direct chemical ionisation-mass spectrometry.
abstract: Series of oligosaccharide ions have been generated from a range of polysaccharides by the application of in-source pyrolysis mass spectrometry, using both ammonia positive-ion chemical ionisation and negative-ion chlorine-nucleophilic-addition ionisation. Glucans with alpha-(1----6), beta-(1----6), alpha-(1----4), beta-(1----4), beta-(1----3), and beta-(1----2) linkages were studied, together with pentosans, xyloglucans, and an arabinogalactan. The series of ions correspond to intact, desorbed oligosaccharides with a terminal anhydro-sugar unit, and to similar oligosaccharides with attached sugar ring-cleavage fragments. The ions generated are dependent on the position of the linkage and ring size, and retain significant information on the structure of the original polysaccharide.
authors.affiliation: Unit for Mass Spectrometry of Macromolecular Systems, FOM Institute for Atomic and Molecular Physics, Amsterdam, The Netherlands.
authors.forename: J A
authors.initials: JA
authors.lastname: Lomax
authors.forename: J J
authors.initials: JJ
authors.lastname: Boon
authors.forename: R A
authors.initials: RA
authors.lastname: Hoffmann
mesh_terms: D002240:Carbohydrate Sequence; D005685:Galactans / Q000737:chemistry; D005936:Glucans / Q000737:chemistry; D006358:Hot Temperature; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry*; D014990:Xylans / Q000737:chemistry
chemical_list: D005685:Galactans; D005936:Glucans; D011134:Polysaccharides; D014990:Xylans; C029353:xyloglucan; C005653:arabinogalactan