title: New approaches to peptide synthesis with the help of trypsin.
abstract: Picolyl esters of amino acids are suitable inverse substrates for trypsin. These esters can be used for peptide synthesis catalyzed by trypsin. Study of hydrolysis of non-specific substrates for trypsin permits to conclude, that hydrogen band in P'2 subsite is very important for their reactivity. Carboxamido methyl esters (CAM-esters) are the most suitable substrates for trypsin catalyzed peptide synthesis. Using CAM-esters and trypsin several di-,tri- and tetra-peptides were synthesized with good yields.
authors.affiliation: Institute of Protein Research, Academy of Sciences, Puschino, Moscow Region, USSR.
authors.lastname: Mitin YuV
authors.forename: N P
authors.initials: NP
authors.lastname: Zapevalova
authors.lastname: Gorbunova EYu
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D004151:Dipeptides / Q000138:chemical synthesis / Q000737:chemistry; D004952:Esters / Q000737:chemistry; D006868:Hydrolysis; D007700:Kinetics; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D010848:Picolinic Acids / Q000737:chemistry; D013379:Substrate Specificity; D014357:Trypsin / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D004151:Dipeptides; D004952:Esters; D010455:Peptides; D010848:Picolinic Acids; D014357:Trypsin