DNA excision repair in mammalian cell extracts.
The many genetic complementation groups of DNA excision-repair defective mammalian cells indicate the considerable complexity of the excision repair process. The cloning of several repair genes is taking the field a step closer to mechanistic studies of the actions and interactions of repair proteins. Early biochemical studies of mammalian DNA repair in vitro are now at hand. Repair synthesis in damaged DNA can be monitored by following the incorporation of radiolabelled nucleotides. Synthesis is carried out by mammalian cell extracts and is defective in extracts from cell lines derived from individuals with the excision-repair disorder xeroderma pigmentosum. Biochemical complementation of the defective extracts can be used to purify repair proteins. Repair of damage caused by agents including ultraviolet irradiation, psoralens, and platinating compounds has been observed. Neutralising antibodies against the human single-stranded DNA binding protein (HSSB) have demonstrated a requirement for this protein in DNA excision repair as well as in DNA replication.
Wood
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Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK.
Coverley
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D000818:Animals; D002460:Cell Line; D002474:Cell-Free System; D004247:DNA / Q000378:metabolism / Q000528:radiation effects; D004249:DNA Damage; D011088:DNA Ligases / Q000502:physiology; D004260:DNA Repair; D004270:DNA, Circular / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000502:physiology; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005816:Genetic Complementation Test; D006801:Humans; D008322:Mammals / Q000378:metabolism*; D008957:Models, Genetic; D008958:Models, Molecular; D010957:Plasmids; D011740:Pyrimidine Dimers / Q000378:metabolism; D011743:Pyrimidines / Q000528:radiation effects; D051716:Replication Protein A; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000145:classification / Q000235:genetics / Q000378:metabolism
D004270:DNA, Circular; D004268:DNA-Binding Proteins; D011740:Pyrimidine Dimers; D011743:Pyrimidines; C497167:RPA1 protein, human; D051716:Replication Protein A; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D011088:DNA Ligases; C030986:pyrimidine