[Catabolism of lipoprotein-X (Lp-X) induced by infusion of 10% intralipid].
In order to clarify the metabolism of Lipoprotein X (Lp-X) induced by intravenous Intralipid 10%, in vitro experiments using purified Lp-X from the sera of the patients receiving Intralipid 10% were carried out. 1) Lp-X or high density lipoprotein (HDL) was incubated with J-774 macrophages laden with [3H] cholesterol. Marked extraction of cholesterol from macrophages by Lp-X as well as HDL was observed. 2) [3H] cholesterol labelled Lp-X or oxidized LDL (o-LDL) was incubated with J-774 macrophages. Incorporation of Lp-X into macrophages was negligible comparing to o-LDL. 3) [3H] cholesterol labelled Lp-X, low density lipoprotein (LDL), or HDL was incubated with Hep G2 cells was less than LDL, but similar to that of HDL. These results indicated that Lp-X extracted cholesterol from peripheral tissues during its formation, and it was not catabolized by the scavenger pathway, but catabolized by the LDL pathway of hepatocytes.
Abe
M
M
First Department of Surgery, School of Medicine, Chiba University, Japan.
Tashiro
T
T
Mashima
Y
Y
Yamamori
H
H
Nishizawa
M
M
Sanada
M
M
Okui
K
K
D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008073:Lipoprotein-X / Q000096:biosynthesis / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism
D005217:Fat Emulsions, Intravenous; D008073:Lipoprotein-X; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D002784:Cholesterol