[The tumorigenicity and metastasis of low-malignancy STHE-strain cells undergoing in vitro selection with peritoneal exudate cells].
We have previously shown that in vitro cocultivation of the low-malignant spontaneously transformed in vitro cells of STHE strain with LPS-activated peritoneal exudate cells (PECa) of normal Syrian hamsters resulted (in contrast to resident PEC (PECr)) in selection of the STHE cell variants resistant to macrophage- and hydrogen peroxide (H2O2)-mediated cytotoxic activity (CTA). In the present study the malignant characteristics, i.e. tumorigenic and metastasizing activities (TGA, MA), of the STHE cell variants in vitro selected with PECa and PECr as well as the parental ones were compared. By the use of quantitative transplantation test the relatively increased level of TGA of the STHE cell variants selected with PECa, especially on the late stages of in vitro selection, has been demonstrated. The increase of the tumour size of transplanted cells was demonstrated after in vitro selection of parental STHE cells with either PECa of PECr. The mean number of spontaneous lung metastases at 60th day after s.c. transplantation of the cell variants selected with PECa and PECr was increased as compared to parental STHE cells, however, as a rule, did not exceed 20-25. The study of experimental MA revealed that only STHE cell variants selected with PECa were able to form significantly larger numbers of experimental lung metastases, what was more expressed after the 4th, 5th and 10th cycles of the selection procedure. Thus, activated REC (mainly macrophages and lymphocytes) are capable to select in vitro tumour cell variants characterized with not only increased resistance to CTA of the macrophages and H2O2, but also more tumorigenic and metastasizing, i.e. more malignant.
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D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D006224:Cricetinae; D003584:Cytological Techniques; D008175:Lung Neoplasms / Q000556:secondary; D008297:Male; D008647:Mesocricetus; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology; D013997:Time Factors
Tumorogennost' i metastazirovanie nizkozlokachestvennykh kletok shtamma KhETR pri ikh otbore in vitro kletkami peritoneal'nogo ékssudata.