title: Calpain 1-protein kinase C complex: effect of calpain inhibitors after dissociation.
abstract: A calpain 1-protein kinase C (PKC) complex was isolated from rabbit skeletal muscle by hydrophobic interaction chromatography on phenyl-sepharose and by strong anion exchange chromatography on Q-Sepharose. Calpain 1 and kinase activities were then dissociated on a phenyl-Sepharose matrix using gradients of decreasing ionic strength. The purified PKC obtained corresponded to conventional PKC and was recognized by a monoclonal antibody specific for alpha and beta isotypes. Leupeptin, calpain inhibitor II, and the more selective calpain inhibitors calpeptin and MDL 28170 did not block the activation of the purified PKC by Ca2+ and phosphatidylserine.
authors.affiliation: ISTAB, Université Bordeaux I, Talence, France.
authors.forename: M
authors.initials: M
authors.lastname: Savart
authors.forename: V
authors.initials: V
authors.lastname: Pallet
authors.forename: P
authors.initials: P
authors.lastname: Letard
authors.forename: C
authors.initials: C
authors.lastname: Bossuet
authors.forename: A
authors.initials: A
authors.lastname: Ducastaing
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002154:Calpain / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D004151:Dipeptides / Q000494:pharmacology; D004789:Enzyme Activation; D004795:Enzyme Stability; D007976:Leupeptins; D008297:Male; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology; D011493:Protein Kinase C / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits
chemical_list: D004151:Dipeptides; D007976:Leupeptins; C071482:calpeptin; D011493:Protein Kinase C; D002154:Calpain