Preparative separation and analysis of the enantiomers of [3H]Abbott-69992, an HIV anti-infective nucleoside, by ligand-exchange high-performance liquid chromatography.
Several chiral stationary phases (CSPs) were examined to separate the enantiomers of A-69992, a chiral HIV anti-infective nucleoside. The only CSP found to be effective was Nucleosil Chiral-1, a ligand-exchange CSP, which was used to prepare microgram amounts of the enantiomers of high optical purity. This appears to be the first separation of the enantiomers of a nucleoside by chiral high-performance liquid chromatography.
Thomas
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Drug Metabolism Department, Abbott Labortories, Abbott Park, IL 60064-3500.
Surber
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D000998:Antiviral Agents / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003503:Cyclobutanes / Q000032:analysis*; D006147:Guanine / Q000031:analogs & derivatives* / Q000032:analysis; D013237:Stereoisomerism; D014316:Tritium
D000998:Antiviral Agents; D003503:Cyclobutanes; D014316:Tritium; C074241:Abbott 69992; D006147:Guanine