SOFI: a bidimensional detector for fast direct on-line quantification of beta particles on blots.
We present a high-speed, high-resolution beta imager developed to replace autoradiographic films currently used in molecular biology experiments. It allows the user to locate and make quantitative analyses of 32P-labeled molecules on a 25.6 x 25.6-cm flat surface. Combining new techniques--scintillating optical fibers and multianode photomultipliers--this fast imager offers several advantages when compared with recent gas detectors and flexibility for further improvements. Several biological applications will be discussed.
Mastrippolito
R
R
Institut de Physique Nucléaire, Université Paris, France.
Bendali
M
M
Charon
Y
Y
Leblanc
M
M
Martin
B
B
Tricoire
H
H
Valentin
L
L
D000818:Animals; D001345:Autoradiography; D001610:Beta Particles; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004330:Drosophila / Q000235:genetics; D005336:Fiber Optic Technology; D015723:Gene Library; D007091:Image Processing, Computer-Assisted; D009693:Nucleic Acid Hybridization; D055100:Optical Fibers; D012441:Saccharomyces cerevisiae / Q000235:genetics