[The translocation and specific binding of the pesticide fluometuron (cotoran) with the proteins of rat liver nuclei].
Intracellular distribution of labeled cotoran was studied. 3H-cotoran was shown to penetrate through the nuclear membrane to accumulate uneventfully in the intranuclear components. An insignificant amount of 3H-cotoran was associated with the nucleoplasm and the outer nuclear membrane. At the same time, essential radioactivity was observed in the proteins of the nuclear matrix (up to 30%) and in non-histone proteins of chromatin (up to 60%). Acception of 3H-cotoran on metaphase chromosomes of cultured cells as well as specificity of cotoran binding with non-histone proteins of chromatin in vivo and in vitro was studied by radioautography. It was revealed that cotoran was translocated into the interphase nuclei to be accepted by metaphase chromosomes of the HeLa line cells and fibroblasts in human embryo, and specifically, in receptor-like manner, bound to chromatin proteins.
Khamidov
D Kh
DKh
Mirakhmedov
A K
AK
Aripdzhanov
A A
AA
Sagatova
G A
GA
Azimova
Sh S
ShS
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006367:HeLa Cells / Q000378:metabolism; D006540:Herbicides / Q000493:pharmacokinetics*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008782:Methylurea Compounds / Q000493:pharmacokinetics*; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D002843:Chromatin; D006540:Herbicides; D008782:Methylurea Compounds; D014316:Tritium; C005015:fluometuron
Translokatsiia i spetsificheskoe sviazyvanie pestitsida fluometurona (kotorana) s belkami iader pecheni krys.