Immunological and molecular genetic analysis of the cellulase component from Penicillium funiculosum.
Following immunization of rabbits, the antiserum was initially analyzed for antiendoglucanase activity using dot-blot ELISA methods. When compared with the preimmune serum, the antiserum showed strong response even at 25 ng concentration. The specificity of the polyclonal antibodies, raised against the partially purified endoglucanase component of Penicillium funiculosum, was determined by Oüchterlony double diffusion. Rocket electrophoresis confirmed the presence of only two types of antigens in the serum. The two rockets obtained were attributable to endo I and the merging of immunologically identical (but mobility wise different) endo II and endo III. This antibody preparation was used as a probe. The deduced M1 of the cloned E. coli endo I was found to be 58 Kd by Western blotting.
Sahasrabudhe
N A
NA
Department of Biochemical Sciences, National Chemical Laboratory, Pune, India.
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D015153:Blotting, Western; D002480:Cellulase / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D011817:Rabbits
D002480:Cellulase