Alpha-1-antitrypsin and alpha-1-antitrypsin-neutrophil elastase complex in bronchoalveolar lavage fluid of patients with pulmonary diseases (pilot study).
A simple method based on rocket and crossed immunoelectrophoresis was adapted for detection of alpha 1 AT-HNE complexes in BALF: Agarose gel with anti-alpha 1 AT combined with intermediate gel containing anti-HNE was used for detection and evaluation of the ratio of complexes with HNE to the free form of alpha 1 AT. BALF samples from 31 patients divided into 5 groups suffering from various respiratory tract diseases were tested. It was found that 3 out of 8 patients with inflammatory pulmonary diseases showed the presence of both forms of alpha 1 AT, 7 out of 13 patients suffering from cancer showed both forms, whereas the complexed form was not detected in any of 8 patients with asthma bronchiale. It is suggested that the presence of alpha 1 AT-HNE complex may reflect, to some extent, the proteolytic status of pulmonary tissue.
Pajdak
W
W
Department of Immunology, Nicolas Copernicus Academy of Medicine, Kraków, Poland.
Sieradzak-Fleituch
M
M
Dubin
A
A
Owsiński
J
J
D000328:Adult; D001249:Asthma / Q000503:physiopathology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D019272:Leukocyte Elastase; D008171:Lung Diseases / Q000503:physiopathology*; D008175:Lung Neoplasms / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000503:physiopathology*; D010196:Pancreatic Elastase / Q000032:analysis*; D010865:Pilot Projects; D011471:Prostatic Neoplasms / Q000503:physiopathology*; D011485:Protein Binding; D000515:alpha 1-Antitrypsin / Q000032:analysis*
D000515:alpha 1-Antitrypsin; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase