High-performance liquid chromatographic separation of the enantiomers of hydroxychloroquine and its major metabolites in biological fluids using an alpha 1-acid glycoprotein stationary phase.
An enantioselective two-stage off-line assay has been developed for the analysis of hydroxychloroquine and its three major metabolites in biological fluids. The first non-stereoselective stage of the assay (PRP-1 column) separates and quantitates parent drug and metabolites. Fractions containing hydroxychloroquine and each of the metabolites are collected manually, evaporated, reconstituted in mobile phase and re-injected onto an alpha 1-acid glycoprotein column to separate and determine proportions of individual enantiomers. Preliminary results from patients samples indicate that the disposition of hydroxychloroquine and its major metabolites is enantioselective. p6
McLachlan
A J
AJ
Department of Pharmacy, University of Sydney, NSW, Australia.
Tett
S E
SE
Cutler
D J
DJ
D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006886:Hydroxychloroquine / Q000378:metabolism*; D009961:Orosomucoid; D013237:Stereoisomerism
D009961:Orosomucoid; D006886:Hydroxychloroquine