Measurement of epidoxorubicin and its metabolites by high-performance liquid chromatography using an advanced automated sample processor.
A sensitive and rapid method for measuring epidoxorubicin and its six metabolites by high-performance liquid chromatography using an advanced automated sample processor is described. Plasma samples (1 ml) were extracted using C2 cassettes, and reversed-phase chromatography was performed with an Apex II ODS column. The isocratic mobile phase of acetonitrile-0.019 M NaH2PO4 (pH 4.0) had a flow-rate of 1 ml/min and the fluorescence detector an excitation wavelength of 480 nm with an emission at 580 nm. Linear calibration curves were obtained which were reproducible both within-day and day-to-day (coefficients of variation less than 10%). The extraction efficacy of epidoxorubicin was 88% and ranged from 51 to 88% for the metabolites. This method has been successfully applied to measure the plasma levels of these compounds in patients receiving epidoxorubicin over a wide dose range (12-120 mg/m2) and in patients with disturbed liver biochemistry.
Dobbs
N A
NA
Imperial Cancer Research Fund, United Medical School, Guy's Hospital, London, UK.
Twelves
C J
CJ
D002851:Chromatography, High Pressure Liquid; D004317:Doxorubicin / Q000031:analogs & derivatives / Q000097:blood; D015251:Epirubicin / Q000031:analogs & derivatives / Q000097:blood*; D005965:Glucuronates / Q000097:blood; D006801:Humans; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D005965:Glucuronates; C074868:epidoxorubicin glucuronide; C074869:7-deoxy-13-dihydroadriamycinone; D015251:Epirubicin; D004317:Doxorubicin; C046579:4'-deoxydoxorubicinol