[Measurement of mitochondrial transmembrane electric potential using the fluorescent probe DSM].
The measurement of transmembrane electric potential of rat liver mitochondria was performed with fluorescent probe DSM. In the incubation medium with succinate the electric potential was -190 +/- 5 mV. Complete uncoupling caused by CCCP in the concentration 0.5 microM decreased the potential to -142 +/- 3 mV. In the partial uncoupling with a half of the mentioned CCCP concentration the potential was -158 +/- 6 mV. DSM influence on oxidation and phosphorylation was not detected.
Kolosova
N G
NG
Kolpakov
A R
AR
D000818:Animals; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000502:physiology; D007700:Kinetics; D008564:Membrane Potentials; D008930:Mitochondria, Liver / Q000502:physiology*; D011726:Pyridinium Compounds; D051381:Rats
D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; C011786:2-(4-(dimethylamino)styryl)-1-methylpyridinium
Izmerenie transmembrannogo élektricheskogo potentsiala mitokhondriĭ s pomoshch'iu fluorestsentnogo zonda DSM.