title: Exploitation of a 650 bp probe for quantification of Pneumocystis carinii.
abstract: Signals obtained from serial dilutions of rat-derived Pneumocystis carinii DNA were used to assess the sensitivity of a 650-bp DNA probe which recognises both cystic and non-cystic forms. Enhanced chemiluminescence was selected as a non-radioactive detection method and signals could be semi-quantitated by scanning densitometry. This technique was used to examine the inhibitory effect of pentamidine in vitro indicating that DNA probes might be useful tools in the search for a novel therapeutic agent.
authors.affiliation: Chemotherapy Department, Glaxo Group Research Ltd., Greenford, Middx, UK.
authors.forename: V
authors.initials: V
authors.lastname: Hancock
authors.forename: C
authors.initials: C
authors.lastname: Christodoulou
authors.forename: H C
authors.initials: HC
authors.lastname: Jackson
mesh_terms: D000818:Animals; D015342:DNA Probes; D004271:DNA, Fungal / Q000302:isolation & purification*; D003720:Densitometry; D008168:Lung / Q000382:microbiology; D011010:Pneumocystis / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D015342:DNA Probes; D004271:DNA, Fungal