Microbial degradation of 2 alpha, 3 alpha-dihydroxy-5 alpha-cholestan-6-one by Mycobacterium vaccae.
2 alpha,3 alpha-Dihydroxy-5 alpha-cholestan-6-one (3), which had the substitution pattern of brassinosteroids in the A/B-ring moiety, was transformed by Mycobacterium vaccae to give 2 alpha,3 alpha,6 alpha-trihydroxy-5 alpha-androstan-17-one (4) and 2 alpha-hydroxyandrost-4-ene-3,17-dione (5). The structures of these compounds were determined by spectroscopic methods, especially 1H nuclear magnetic resonance studies.
Vorbrodt
H M
HM
Institute of Plant Biochemistry, Halle, Weinbergweg, Germany.
Adam
G
G
Porzel
A
A
Hörhold
C
C
Dänhardt
S
S
Böhme
K H
KH
D001711:Biotransformation; D002778:Cholestanones / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009161:Mycobacterium / Q000378:metabolism*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D002778:Cholestanones; C075232:2,3-dihydroxycholestan-6-one