title: Rapid identification of Escherichia coli from urine by using Fluorocult media.
abstract: For rapid identification of Escherichia coli, we evaluated Fluorocult MacConkey Agar, Fluorocult Laurylsulfate Broth and Bactident E. coli, which are incorporating fluorogenic substrate, MUG (4-methylumbeliferyl-beta-D-Glucuronide) that specifically reacts with E. coli. To assess the specificity and sensitivity of Fluorocult MacConkey Agar and Laurylsulfate Broth, beta-D-glucuronidase; beta-GUR activities of 264 strains from urine including 72 of E. coli were investigated. For both media, sensitivity was 92% and specificity was 100%. When there was 10(8) c.f.u./ml of E. coli in urine specimen, incubation times required for positive fluorescence by Fluorocult MacConkey Agar, Laurylsulfate Broth, and Bactident E. coli were 8 h, 4 h and 15 min, respectively. Influence of drugs in urine to fluorescence reaction was not observed.
authors.affiliation: Central Clinical Laboratories, Mitsui Memorial Hospital, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: E
authors.initials: E
authors.lastname: Maehata
authors.forename: H
authors.initials: H
authors.lastname: Naka
mesh_terms: D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D015169:Colony Count, Microbial; D003470:Culture Media; D003556:Cystitis / Q000382:microbiology / Q000652:urine; D004926:Escherichia coli / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000652:urine; D005069:Evaluation Studies as Topic; D005456:Fluorescent Dyes; D006801:Humans; D006923:Hymecromone / Q000031:analogs & derivatives; D012680:Sensitivity and Specificity; D014556:Urine / Q000382:microbiology*
chemical_list: D003470:Culture Media; D005456:Fluorescent Dyes; D006923:Hymecromone; C034888:4-methylumbelliferyl glucuronide