title: Urinary growth hormone excretion in 657 healthy children and adults: normal values, inter- and intraindividual variations.
abstract: Urinary growth hormone (u-GH) excretion was measured in 547 healthy children and 110 adults by ELISA with a detection limit of 1.1 ng/l u-GH after prior concentration of the urine samples (20- to 30-fold). u-GH excretion values were significantly dependent on the pubertal stage (p less than 0.0001) with maximum values in Tanner stage 3 for girls and 4 for boys. This corresponded to a peak in u-GH excretion between 11.5-14.5 years in girls and 12.5-16 years in boys. Additionally, u-GH excretion in adults was significantly higher than in prepubertal children (p less than 0.001). The day/night ratio of u-GH excretion (pg/h) was significantly higher in females than in males (p less than 0.01). In Tanner stages 1-4, u-GH excretion during the day was lower than that at night, whereas the opposite was true in late puberty and in adult women. The interindividual variation of u-GH excretion within the same Tanner stage was considerable and approximately double the intraindividual variation. The day-to-day variation could be further reduced by collection of three consecutive urine samples. The variations were larger if night samples instead of 24-hour samples were considered. The expression of u-GH excretion in nanograms per gram creatinine did not diminish the observed variation and blunted the pubertal increase in u-GH excretion. In conclusion, (1) u-GH excretion depends significantly on age, sex and pubertal maturation as does the day/night ratio of u-GH excretion. (2) The interindividual variation in u-GH excretion is considerable.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: University Department of Growth and Reproduction, Rigshospitalet, Copenhagen, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Main
authors.forename: M
authors.initials: M
authors.lastname: Philips
authors.forename: M
authors.initials: M
authors.lastname: Jørgensen
authors.forename: N E
authors.initials: NE
authors.lastname: Skakkebaek
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002940:Circadian Rhythm; D003430:Cross-Sectional Studies; D003718:Denmark; D005260:Female; D013006:Growth Hormone / Q000652:urine*; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D011627:Puberty / Q000652:urine; D012016:Reference Values; D012727:Sex Characteristics
chemical_list: D013006:Growth Hormone