title: The analysis of thiocarbamides by gas chromatography/negative-ion chemical-ionization mass spectrometry.
abstract: Thiocarbamides were converted to their di-N-pentafluorobenzyl (PFB) derivatives and analysed by gas chromatography/negative-ion chemical-ionization mass spectrometry with methane as reagent gas. The PFB derivatives of the 2-thiouracils gave mass spectra in which the ion current was carried largely by an ion arising from [M-PFB]-. The derivative was used in the determination of the uptake and metabolism of thiocarbamides by cultures of melanoma cells.
authors.affiliation: Department of Pharmacy, University of Strathclyde, Glasgow, UK.
authors.forename: D G
authors.initials: DG
authors.lastname: Watson
authors.forename: C D
authors.initials: CD
authors.lastname: Bates
authors.forename: G G
authors.initials: GG
authors.lastname: Skellern
authors.forename: R
authors.initials: R
authors.lastname: Mairs
authors.forename: S
authors.initials: S
authors.lastname: Martin
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008545:Melanoma / Q000378:metabolism*; D008713:Methimazole / Q000493:pharmacokinetics; D008779:Methylthiouracil / Q000493:pharmacokinetics*; D011441:Propylthiouracil / Q000493:pharmacokinetics*; D013889:Thiouracil / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D014407:Tumor Cells, Cultured
chemical_list: D008713:Methimazole; D013889:Thiouracil; D011441:Propylthiouracil; C019269:benzylthiouracil; D008779:Methylthiouracil