Effect of dietary fat on rat liver microsomal and mitochondrial/lysosomal dolichol, phospholipid and cholesterol.
The influence of different fat diets on liver phospholipid, cholesterol and dolichol was studied. Rats were separated into four groups and fed standard laboratory chow (control), a diet containing linolenic acid, a coconut oil diet, or a corn oil-containing diet. After five weeks, microsomes and mitochondrial/lysosomal fractions were prepared from the liver, and lipid compositions were analyzed. No changes in phospholipid content were observed. In control animals, the fatty acid compositions of phosphatidylcholine and phosphatidylethanolamine in the two subfractions were similar. However, these two phospholipids showed different fatty acid patterns, which were altered independently upon dietary treatment. The dietary treatments resulted, in most cases, in decreased cholesterol and dolichol contents and, especially in microsomes, in a decreased level of esterification of both lipids. The fatty acid compositions of cholesteryl esters in the two subfractions showed significant differences and cholesterol was esterified to a large extent with linolenic acid when this fatty acid was supplied in the diet. The same dietary treatment exerted different effects on the cholesterol localized in the two different intracellular compartments. This difference was most pronounced in rats fed the corn oil-containing diet; microsomal cholesteryl esters exhibited increased saturation, whereas cholesteryl esters exhibited increased saturation, whereas cholesteryl esters in the mitochondrial/lysosomal fraction displayed decreased saturation. Dolichyl esters in the two cellular compartments had different fatty acyl compositions, with a considerably higher degree of saturation in microsomes. The various diets influenced the nature of the fatty acid moieties present in the isolated fractions and the effects on the two subfractions were opposite.(ABSTRACT TRUNCATED AT 250 WORDS)
Jakobsson-Borin
A
A
Department of Biochemistry, Arrhenius Laboratories, University of Stockholm, Sweden.
Tollbom
O
O
Dallner
G
G
D000818:Animals; D002784:Cholesterol / Q000032:analysis; D000074263:Coconut Oil; D003314:Corn Oil / Q000494:pharmacology; D004041:Dietary Fats / Q000494:pharmacology*; D004286:Dolichols / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008042:Linolenic Acids / Q000494:pharmacology; D008055:Lipids / Q000032:analysis*; D008099:Liver / Q000737:chemistry* / Q000187:drug effects; D008247:Lysosomes / Q000737:chemistry / Q000187:drug effects; D008297:Male; D008861:Microsomes / Q000737:chemistry / Q000187:drug effects; D008928:Mitochondria / Q000737:chemistry / Q000187:drug effects; D010743:Phospholipids / Q000032:analysis; D010938:Plant Oils / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000737:chemistry* / Q000187:drug effects
D004041:Dietary Fats; D004286:Dolichols; D005227:Fatty Acids; D008042:Linolenic Acids; D008055:Lipids; D010743:Phospholipids; D010938:Plant Oils; D003314:Corn Oil; D002784:Cholesterol; D000074263:Coconut Oil