Urease purification from the seeds of Cajanus cajan and its application in a biosensor construction.
Urease has been purified from the seeds of Cajanus Cajan. The purification process involves three solvent extraction steps followed by DEAE-cellulose column chromatography. The specific activity of the purified enzyme is found to be 1920 U/mg with the recovery of 8%. The application of the purified enzyme in a biosensor construction is discussed.
Pandey
P C
PC
Department of Chemistry, Banaras Hindu University, Varanasi, India.
Pandey
V
V
D015374:Biosensing Techniques; D012639:Seeds / Q000201:enzymology*; D014510:Urease / Q000302:isolation & purification*
D014510:Urease