title: In vitro bioassay for the brain allatotropic hormone of Galleria mellonella (Lepidoptera).
abstract: An in vitro sensitive bioassay for the Galleria mellonella brain allatotropic hormone (ATTH) was developed. This assay measures the rate of juvenile hormone (JH) synthesis in corpora cardiacacorpora allata complex (CC-CA) stimulated in vitro by ATTH released from the brain during short-term in vitro incubation, or by ATTH extracted from the tissue with methanol. CC-CA of the late VIth instar (VI3) larvae were used for assessment of ATTH. The maximum activation of test CC-CA by ATTH occurred at a concentration of 2 brain equivalents (per 100 ul medium). The highest ATTH activity was exhibited by the brains of chilled VII1 larvae: ATTH extracted from freshly dissected brains, or ATTH released from these brains during 6 h in vitro incubation, activated JH synthesis in the CC-CA nearly five or four times, respectively. The brain of VII1 hydroprenetreated larvae were ATTH inactive.
authors.affiliation: Department of Invertebrate Physiology, Warsaw University, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Muszyńska-Pytel
authors.forename: E
authors.initials: E
authors.lastname: Szołajska
authors.forename: M A
authors.initials: MA
authors.lastname: Pszczółkowski
authors.forename: J
authors.initials: J
authors.lastname: Michalik
authors.forename: P
authors.initials: P
authors.lastname: Mikołajczyk
mesh_terms: D000818:Animals; D001681:Biological Assay; D001923:Brain Chemistry; D003335:Corpora Allata / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D066298:In Vitro Techniques; D007301:Insect Hormones / Q000032:analysis*; D007605:Juvenile Hormones / Q000096:biosynthesis; D007814:Larva; D007915:Lepidoptera / Q000737:chemistry* / Q000378:metabolism; D013997:Time Factors
chemical_list: D005231:Fatty Acids, Unsaturated; D007301:Insect Hormones; D007605:Juvenile Hormones; C006481:ethyl-3,7,11-trimethyl-2,4-dodecadienoate