Protective action of a new benzofuran derivative on lipid peroxidation and sulphydryl groups oxidation.
The antioxidant properties of a novel water-soluble antioxidant of the benzofuran family (5-hydroxy-4,6,7-trimethyl-2,3-dihydrobenzofuran-2-acetic acid, BFA) were studied. In rat liver mitochondria BFA increases the lag-time and decreases the extent of lipid peroxidation induced by ascorbate/Fe2+; an IC50 value of about 12 microM was observed. In rat liver microsomes it inhibits the lipid peroxidation induced both by NADPH/Fe2+/ADP (iron-dependent) and by cumene hydroperoxide (iron-independent), showing IC50 values of 25 and 30 microM respectively. The antioxidant efficiency of BFA is slightly higher than that of the congener compound Trolox C. BFA is also able to inhibit the oxidation of protein sulphydryl groups consequent to microsomal lipid peroxidation induced by NADPH/Fe2+/ADP. The antioxidant properties of BFA are discussed considering its hydrophilic character and pharmacological features.
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Centro Studio Fisiologia Mitocondriale (CNR), Padova, Italy.
Rigobello
M P
MP
Musacchio
E
E
Scuri
R
R
Rizzoli
V
V
Galzigna
L
L
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001555:Benzene Derivatives / Q000494:pharmacology; D001572:Benzofurans / Q000737:chemistry / Q000494:pharmacology*; D002839:Chromans / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D009249:NADP / Q000494:pharmacology; D010084:Oxidation-Reduction; D051381:Rats; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D000975:Antioxidants; D001555:Benzene Derivatives; D001572:Benzofurans; D002839:Chromans; D005296:Ferrous Compounds; D013438:Sulfhydryl Compounds; C073918:IRFI 005; D009249:NADP; D000244:Adenosine Diphosphate; C007164:cumene hydroperoxide; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid