[A structure-activity study of the HindIII-I fragment of the L-IVP strain of vaccinia virus genome. I. Cloning of T5 gene and identification of its protein product].
The I5 gene from the HindIII-I-fragment of the vaccinia virus strain L-IVP DNA was cloned into bacterial vector pUC19. The monospecific antiserum to the expression product of this gene in E. coli was obtained. This antiserum was demonstrated to co-precipitate the virion protein p90. The vaccinia virus strain L-LVP DNA was shown to have only one ORF coding the p90 protein instead of two ORF H5 and H4 as known for vaccinia virus strain WR. This protein is associated with the core of vaccinia virion, but some of its antigenic determinants are exposed on the surface of the viral particles.
Netesova
N A
NA
Muravlev
A I
AI
Chikaev
N A
NA
Malygin
E G
EG
D015153:Blotting, Western; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D015247:Deoxyribonuclease HindIII; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D005814:Genes, Viral; D007120:Immunochemistry; D016366:Open Reading Frames; D010957:Plasmids; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D013329:Structure-Activity Relationship; D014616:Vaccinia virus / Q000235:genetics*; D014771:Virion
D004279:DNA, Viral; D015247:Deoxyribonuclease HindIII
Strukturno-funktsional'noe issledovanie HindIII-I-fragmenta genoma virusa ospovaktsiny shtamma L-IVP. I. Klonirovanie gena T5 i identifikatsiia ego belkovogo produkta.