title: The porcine family of interferon-omega: cloning, structural analysis, and functional studies of five related genes.
abstract: We report the molecular characterization of the porcine IFN-omega multigenic family which appears to comprise six to seven loci. Five of these loci were cloned and sequenced, revealing two pseudogenes (psi PoIFN-omega 1 and -omega 2) and three genes with an intact reading frame (PoIFN-omega 3, -omega 4, and -omega 5) that could encode preproteins of 179-190 amino acids including a putative signal peptide of 23 residues. By comparison of porcine IFN-omega coding sequences to those of known mammalian counterparts, it appeared that porcine sequences contain an in-frame five-codon deletion (between positions 113 and 117) in a region of relatively high sequence variability. In vitro transcription and translation of the three potentially functional reading frames gave rise to proteins with antiviral activity, showing that the porcine-specific deletion does not abolish the biological activity. Comparative analysis of flanking sequences revealed unique features of the 3' untranslated region of IFN-omega 4 gene: It contained a consensus estrogen-responsive element (ERE) in the vicinity of an extensive A-T-rich sequence known to serve a specific regulatory role in the expression of many genes involved in the inflammatory response. This finding suggests that the expression of the functional members of the porcine IFN-omega family may be mediated through different mechanisms. The expression of these genes was studied by Northern blot analysis of mRNAs from pig conceptus (days 14-20). Five bands of poly(A)+ RNAs hybridized with an IFN-omega probe provided the stringency was low, suggesting that a distinct family of IFN-omega-related genes were expressed by porcine trophoblastic cells during early gestation.
authors.affiliation: I.N.R.A., Laboratoire de Virologie et d'Immunologie Moléculaires, Centre de Jouy, Jouy-en-Josas, France.
authors.forename: D
authors.initials: D
authors.lastname: Mege
authors.forename: F
authors.initials: F
authors.lastname: Lefevre
authors.forename: C
authors.initials: C
authors.lastname: Labonnardiere
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D005260:Female; D015870:Gene Expression; D007370:Interferon Type I / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D016364:Reading Frames; D013329:Structure-Activity Relationship; D013552:Swine; D014327:Trophoblasts
chemical_list: D007370:Interferon Type I; D012333:RNA, Messenger; C063743:interferon omega 1