Clostripain: production and use for peptide synthesis.
The arginine-specific protease Clostripain could efficiently be produced following improved fermentation and isolation procedures. Preparative scale kinetically controlled peptide synthesis of -Arg-Pro- and Arg-Xaa- containing peptides could successfully be performed in high yields with free and immobilized enzyme preparations.
Meiwes
J
J
Hoechst AG, Frankfurt am Main.
Müller
W
W
Grabley
S
S
Schudok
M
M
D000595:Amino Acid Sequence; D003013:Clostridium / Q000201:enzymology; D003546:Cysteine Endopeptidases / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D004800:Enzymes, Immobilized; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry
D004800:Enzymes, Immobilized; D009842:Oligopeptides; D010455:Peptides; D003546:Cysteine Endopeptidases; C019913:clostripain