Immobilization of papain on epoxy-polymers for papain-catalyzed peptide synthesis.
In this paper we describe the immobilization of papain on two commercially available carriers, VA Biosynth and Eupergit C, both in their epoxidized form. Papain can be immobilized on both polymers and shows high activity. The stability (lyophilization, storage) of the papain-VA Biosynth is very good. Even after prolonged storage (-20 degrees C) it can be reactivated to its full activity.
Eckstein
H
H
Institute of Organic Chemistry, University of Tübingen, FRG.
Renner
H J
HJ
Brun
H
H
D002384:Catalysis; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry*; D010206:Papain / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D011108:Polymers
D004800:Enzymes, Immobilized; D010455:Peptides; D011108:Polymers; C065843:Eupergit C; D010206:Papain