Detection of Pneumocystis carinii DNA by polymerase chain reaction compared to direct microscopy and immunofluorescence.
The polymerase chain reaction (PCR) using published primers and probes has been compared to conventional stains and immunofluorescence for diagnosis of Pneumocystis carinii. We have screened 71 bronchoalveolar lavage (BAL) fluids from HIV-immunosuppressed patients. Of 34 samples negative by conventional stains and immunofluorescence, only one was positive by PCR. Thirty of 35 samples positive by conventional stains and immunofluorescence were also positive by PCR. One BAL sample, negative by conventional stains but positive by immunofluorescence, was negative by PCR. These data are discussed in relation to clinical and therapeutic conditions of the patients.
Galan
F
F
Laboratoire de Biochimie, CHU Saint Antoine, Paris, France.
Oliver
J L
JL
Roux
P
P
Poirot
J L
JL
Bereziat
G
G
D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D004271:DNA, Fungal / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D015658:HIV Infections / Q000150:complications; D006801:Humans; D008853:Microscopy; D011010:Pneumocystis / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000150:complications / Q000382:microbiology; D016133:Polymerase Chain Reaction
D004271:DNA, Fungal