title: 19-Nordeoxycorticosterone synthesis by rat kidney inner medullary collecting duct cells.
abstract: 19-Nordeoxycorticosterone (19-nor-Doc), a potent mineralocorticoid, was found to be synthesized by the isolated rat kidney perfused by an adrenal precursor (19-oxo-Doc). To determine if this bioconversion is a function of renal tubular cells, various adrenal precursors of 19-nor-Doc were added separately to rat kidney inner medullary collecting duct cells culture media at a concentration of 10 nM. While 4.6% +/- 1.0% of 19-oxo-Doc (n = 3) and 14.4% +/- 1.4% of 19-oic-Doc (n = 3) were converted to 19-nor-Doc after 24 hours of incubation, Doc, and 19-OH-Doc were not converted. This represents further evidence that Doc has to be metabolized to 19-oxo-Doc or 19-oic-Doc (19-carboxy-Doc) before it can be converted by the kidney inner medullary collecting duct cells to 19-nor-Doc.
authors.affiliation: Evans Medical Foundation, Department of Clinical Research and Medicine, University Hospital, Boston, Massachusetts.
authors.forename: S T
authors.initials: ST
authors.lastname: Azar
authors.forename: J C
authors.initials: JC
authors.lastname: Melby
authors.forename: T E
authors.initials: TE
authors.lastname: Wilson
authors.forename: R
authors.initials: R
authors.lastname: Nichols
authors.forename: J H
authors.initials: JH
authors.lastname: Schwartz
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002478:Cells, Cultured; D003900:Desoxycorticosterone / Q000031:analogs & derivatives* / Q000096:biosynthesis; D007679:Kidney Medulla / Q000166:cytology / Q000378:metabolism*; D007685:Kidney Tubules, Collecting / Q000166:cytology / Q000378:metabolism*; D051381:Rats
chemical_list: D003900:Desoxycorticosterone; C019026:19-nordeoxycorticosterone