title: Urease purification from the seeds of Cajanus cajan and its application in a biosensor construction.
abstract: Urease has been purified from the seeds of Cajanus Cajan. The purification process involves three solvent extraction steps followed by DEAE-cellulose column chromatography. The specific activity of the purified enzyme is found to be 1920 U/mg with the recovery of 8%. The application of the purified enzyme in a biosensor construction is discussed.
authors.affiliation: Department of Chemistry, Banaras Hindu University, Varanasi, India.
authors.forename: P C
authors.initials: PC
authors.lastname: Pandey
authors.forename: V
authors.initials: V
authors.lastname: Pandey
mesh_terms: D015374:Biosensing Techniques; D012639:Seeds / Q000201:enzymology*; D014510:Urease / Q000302:isolation & purification*
chemical_list: D014510:Urease