title: Solid-phase peptide synthesis without side-chain hydroxyl protection of threonine.
abstract: A peptide containing four threonine residues was synthesised by the solid-phase method using fluorenyl-methoxycarbonylamino acid reactive esters or coupling by preactivation with 1-hydroxybenzotriazole and Castro's reagent. In two separate experiments the synthesis was carried out with or without protection of the side-chain hydroxyl group of threonine as the tert.-butyl ether. Comparison of the crude peptides after deprotection and detachment from the synthesis resin suggests that side-chain protection of threonine is unnecessary under the synthetic conditions employed.
authors.affiliation: Deakin Research Ltd., University of Western Sydney, Richmond, NSW, Australia.
authors.forename: P M
authors.initials: PM
authors.lastname: Fischer
authors.forename: K V
authors.initials: KV
authors.lastname: Retson
authors.forename: M I
authors.initials: MI
authors.lastname: Tyler
authors.forename: M E
authors.initials: ME
authors.lastname: Howden
mesh_terms: D009842:Oligopeptides / Q000737:chemistry*; D013912:Threonine / Q000737:chemistry*
chemical_list: D009842:Oligopeptides; D013912:Threonine