Morphology, differentiation and matrix production of liver cells in organoid cultures (high density cultures) of fetal rat livers.
The aim of this study was to demonstrate the morphology and matrix synthesis of embryonic rat liver cells (day 18 of gestation) in organoid cultures (high density cultures) with electron microscopic and immunomorphological techniques. For this purpose the cells of embryonic rat livers were isolated enzymatically and grown in an organoid culture (high density culture) for 3 weeks in a Trowell system. During the first 48 h a sorting-out process took place, i.e. liver and blood-forming cells met to form aggregates. In between mesenchymal cells were seen. Vessel-like cavities developed. Electron microscopic inspection of the hepatocytes did not reveal any lesions of the cell organelles after 14 days in culture. As late as after a 3-week culture period mitochondrial swellings and an increased number of autophagic vacuoles were observed. A rim of collagenous fibrils or fibrillar bundles and granular matrix structures was perceptible as early as after 7 days in culture. Immunofluorescence microscopic techniques revealed collagen types III, IV and VI as well as laminin, nidogen, heparansulfate-proteoglycan and fibronectin in these areas. Thus, the composition of the matrix in this culture system corresponds (apart from the absence of collagen type I) to the embryonic situation. Therefore, the organoid culture appears to be an appropriate technique to study the behaviour of hepatocytes in vitro. It is especially suited to demonstrate the formation of matrix components in liver cells and their extracellular occurrence.
Elkasaby
A
A
Institut für Toxikologie und Embryonal-Pharmakologie, Freie Universität Berlin, FRG.
Xu
D
D
Schröter-Kermani
C
C
Merker
H J
HJ
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D005333:Fetus / Q000166:cytology; D005353:Fibronectins / Q000032:analysis; D005455:Fluorescent Antibody Technique; D007797:Laminin / Q000032:analysis; D008099:Liver / Q000166:cytology* / Q000196:embryology / Q000648:ultrastructure; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis; D008854:Microscopy, Electron; D008930:Mitochondria, Liver / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains
D005353:Fibronectins; D007797:Laminin; D008562:Membrane Glycoproteins; C029967:nidogen; D003094:Collagen