title: HPLC determination of doxorubicin, doxorubicinol and four aglycone metabolites in plasma of AIDS patients.
abstract: A high-performance liquid chromatographic (HPLC) assay has been developed for the determination of the anticancer drug doxorubicin and the metabolites doxorubicinol, doxorubicinone, 7-deoxydoxorubicinone, doxorubicinolone and 7-deoxydoxorubicinolone in plasma of AIDS patients. Samples can be heated at 60 degrees C for 30 min to inactivate the human immunodeficiency virus. The sample pre-treatment involves a liquid-liquid extraction of the buffered plasma sample (pH 9) with a chloroform-1-propanol (4:1, v/v) mixture. The chromatographic analysis is performed on a Lichrosorb RP-8 (5 microns) column and by isocratic elution with a mobile phase of acetonitriletetrahydrofuran-phosphate buffer (pH 2.2) (800:5:200, w/w/w) with fluorescence detection (excitation wavelength: 460 nm; emission wavelength: 550 nm). The proposed method has been validated and, subsequently, implemented in a pharmacokinetic study of doxorubicin in AIDS patients with Kaposi's sarcoma who are treated with the combination regimen doxorubicin, vincristine and bleomycin.
authors.affiliation: Department of Pharmacy, Slotervaart Hospital/Netherlands Cancer Institute, Amsterdam.
authors.forename: J H
authors.initials: JH
authors.lastname: Beijnen
authors.forename: P L
authors.initials: PL
authors.lastname: Meenhorst
authors.forename: R
authors.initials: R
authors.lastname: van Gijn
authors.forename: M
authors.initials: M
authors.lastname: Fromme
authors.forename: H
authors.initials: H
authors.lastname: Rosing
authors.forename: W J
authors.initials: WJ
authors.lastname: Underberg
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D015394:Molecular Structure; D015203:Reproducibility of Results
chemical_list: D004317:Doxorubicin; C010013:adriamycinol