title: Visualization of proteases within a complex sample following their selective retention on immobilized bacitracin, a peptide antibiotic.
abstract: A method for the visualization of individual proteases within a complex biological sample is described. In a single chromatographic step, proteases can be separated from other biomolecules by selective binding to immobilized bacitracin, a peptide antibiotic. Following desorption, proteases may be separated by SDS-polyacrylamide gel electrophoresis. The application of this method is presented in the visualization of proteases secreted by the fungus Aspergillus niger.
authors.affiliation: TNO-Medical Biological Laboratory, Rijswijk, The Netherlands.
authors.forename: J M
authors.initials: JM
authors.lastname: van Noort
authors.forename: P
authors.initials: P
authors.lastname: van den Berg
authors.forename: I E
authors.initials: IE
authors.lastname: Mattern
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology / Q000378:metabolism; D001414:Bacitracin / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000737:chemistry* / Q000172:deficiency / Q000302:isolation & purification; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D012685:Sepharose
chemical_list: D010455:Peptides; D001414:Bacitracin; D012685:Sepharose; D010450:Endopeptidases