[The influence of Chinese Agkistrodon acutus enzyme (CAAE) on the functions of washed human platelets].
The effects of the activation and influence of CAAE on washed human platelets were investigated. Our findings were: (1) CAAE could induce aggregation and alpha-granule release reaction in the washed human platelet suspension; thus it could activate the platelets; (2) the aggregation effect of CAAE was inhibited by albumin and affected by Ca2+, Mg2+ concentration; (3) heparin and/or AT III did not inhibit the aggregation activation of CAAE, but alpha 2MG markedly inhibited it; (4) the aggregation activity of CAAE was associated with the serine protease activity. The mechanism of CAAE-induced aggregation mainly follows the prostaglandins-endoperoxides thromboxane A2 pathway.
Deng
C
C
He
J
J
Yue
S
S
Deng
C
C
Guan
J
J
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism / Q000502:physiology*; D003435:Crotalid Venoms; D006801:Humans; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D010978:Platelet Factor 4 / Q000378:metabolism; D012697:Serine Endopeptidases / Q000494:pharmacology*
D003435:Crotalid Venoms; D010975:Platelet Aggregation Inhibitors; D010978:Platelet Factor 4; D012697:Serine Endopeptidases