Endothelin-1 and endothelin-3 stimulate prostaglandin E2 secretion from the rat median eminence.
The in vitro effects of endothelin-1 (ET-1) and endothelin-3 (ET-3) on the release of prostaglandin (PG)E2 from the rat median eminence were investigated. The addition of ET-1 from 10(-9) M to 10(-6) M stimulated PGE2 release in a dose-dependent manner (from 10.5 +/- 2.1 to 54.4 +/- 5.6 pg/ME fragment/30 min; mean +/- SEM, p less than 0.001). ET-3 also stimulated the release of PGE2 from 10(-7) M to 10(-5) M dose dependently (from 18.1 +/- 0.7 to 60.9 +/- 17.4 pg/ME fragment/30 min p less than 0.05). The time course effect of ET-3 (10(-6) M) showed that PGE2 release was stimulated within five minutes (control, 1.5 +/- 0.5; ET-3, 15.8 +/- 3.0 pg/ME fragment/5 min, p less than 0.01). These results suggest that ET-1 and ET-3 have some physiological effects on the rat median eminence.
Osuga
Y
Y
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Japan.
Mitsuhashi
N
N
Mizuno
M
M
D000818:Animals; D015232:Dinoprostone / Q000378:metabolism*; D016232:Endothelins / Q000502:physiology*; D008297:Male; D008473:Median Eminence / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D016232:Endothelins; D015232:Dinoprostone