Effects of genistein on ATP induced DNA synthesis and intracellular alkalinization in Chang liver cells.
Extracellular ATP (adenosine 5'-triphosphate) produces a dose-dependent elevation of DNA synthesis concomitantly with raised intracellular pH (pHi) in quiescent monolayer Chang liver cells. Genistein produces a dose-dependent suppression of ATP-induced DNA synthesis without affecting cell viability (dead cell index approximately 5%) or pHi elevation. The results suggest that (a) mitogenesis and pHi are not imperative correlates and (b) genistein is effective in inhibiting DNA synthesis whether due to growth factor or "non-growth factor" stimulation.
Sit
K H
KH
Department of Anatomy, National University of Singapore, Kent Ridge.
Wong
K P
KP
Bay
B H
BH
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D019833:Genistein; D006863:Hydrogen-Ion Concentration; D007529:Isoflavones / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*
D007529:Isoflavones; D000255:Adenosine Triphosphate; D004247:DNA; D019833:Genistein; D011505:Protein-Tyrosine Kinases