title: [Production of monoclonal antibodies to staphylococcal alpha-toxin using the technique of in vitro immunization].
abstract: The aim of this study is production of monoclonal antibodies (MAB) to staphylococcal alpha-toxin (SAT). SAT was obtained from culture medium of S. aureus s. 0-15 with ion-exchange chromatography and chromatofocusing. SAT was conjugated with CH-sepharose 4B and used for in vitro immunization of spleen cells, extracted from intact BALB/c mouse.
authors.forename: N N
authors.initials: NN
authors.lastname: Beliaev
authors.forename: G K
authors.initials: GK
authors.lastname: Zakir'ianova
authors.forename: G O
authors.initials: GO
authors.lastname: Kostin
authors.forename: R T
authors.initials: RT
authors.lastname: Tleulieva
authors.forename: A O
authors.initials: AO
authors.lastname: Agashkin
authors.forename: D G
authors.initials: DG
authors.lastname: Navasardiants
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Ezepchuk
authors.forename: A B
authors.initials: AB
authors.lastname: Beklemishev
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins / Q000276:immunology*; D006460:Hemolysin Proteins / Q000276:immunology*; D007114:Immunization; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009498:Neurotoxins / Q000276:immunology*; D013210:Staphylococcus / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D009498:Neurotoxins; C021091:staphylococcal alpha-toxin
vernacular_title: Poluchenie monoklonal'nykh antitel k stafilokokkovomu alfa-toksinu pri ispol'zovanii tekhniki immunizatsii in vitro.