Thin-layer chromatography of urinary 17-oxosteroids using dansylhydrazine as a prelabelling reagent.
This paper describes a modification of a high-performance liquid chromatographic method for measurement of 17-oxosteroids in biological fluids for use with thin-layer chromatography and fluorometric scanning detection. After extraction from urine samples with Separon-C18 microcolumns, free oxosteroids were labelled with dansylhydrazine in acetonitrile-acetic acid and chromatographed on silica gel F-254 plates with the solvent system chloroform-methanol (97:3). Linearity of fluorescence detection (Shimadzu CS-9000 densitometer) was obtained between 30 and 1000 ng.
Tomsová
Z
Z
3rd Medical Department, Charles University, Prague, Czechoslovakia.
D015068:17-Ketosteroids / Q000652:urine*; D002855:Chromatography, Thin Layer; D003619:Dansyl Compounds / Q000737:chemistry*; D006801:Humans; D006834:Hydrazines / Q000737:chemistry*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D015068:17-Ketosteroids; D003619:Dansyl Compounds; D006834:Hydrazines; C027913:dansyl hydrazine