Determination of zopiclone enantiomers in plasma by liquid chromatography using a chiral cellulose carbamate column.
The enantiomers of zopiclone were determined in human plasma using a sequential achiral-chiral liquid chromatographic method. Zopiclone was separated from the biological matrix and quantified on an achiral silica column. The limit of detection was 5 ng/ml. The eluent fraction containing zopiclone was collected, evaporated, reconstituted with the mobile phase and injected onto a chiral cellulose carbamate column where the enantiomeric ratio was calculated. This validated method, applied to a pilot study, suggests that pharmacokinetics of zopiclone is stereoselective.
Fernandez
C
C
Hôpital Pitié Salpétrière, Service Pharmacie, Paris, France.
Baune
B
B
Gimenez
F
F
Thuillier
A
A
Farinotti
R
R
D053961:Azabicyclo Compounds; D002219:Carbamates; D002482:Cellulose; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D006801:Humans; D006993:Hypnotics and Sedatives / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D010879:Piperazines / Q000097:blood* / Q000493:pharmacokinetics; D013237:Stereoisomerism
D053961:Azabicyclo Compounds; D002219:Carbamates; D006993:Hypnotics and Sedatives; D010879:Piperazines; C515050:zopiclone; D002482:Cellulose