Oxidative and assimilative enzyme activities in continuous cultures of the obligate methylotroph Methylobacillus flagellatum.
In methanol-limited continuous cultures of the obligate methylotrophic bacterium Methylobacillus flagellatum grown at rates from 0.05 to 0.63 h-1, and also in an oxyturbidostat culture of M. flagellatum growing at the rate of 0.73 h-1, levels of methanol dehydrogenase, enzymes of formaldehyde oxidation (both linear and cyclic) and assimilation (RuMP cycle), a number of intermediary metabolism and TCA cycle enzymes and also 'dye-linked' formaldehyde dehydrogenase were determined. It was shown that the activities of dissimilatory enzymes, with the exception of 'dye-linked' formaldehyde dehydrogenase, decreased with increasing growth rate. Activities of assimilative enzymes and activities of the TCA cycle enzymes detected as well as the 'dye-linked' formaldehyde dehydrogenase activity, increased with increasing growth rate. A periplasmic location was shown for the latter enzyme and a role in formaldehyde detoxification was proposed.
Chistoserdova
L V
LV
Institute of Genetics and Selection of Industrial Microorganisms, Moscow, USSR.
Chistoserdov
A Y
AY
Schklyar
N L
NL
Baev
M V
MV
Tsygankov
Y D
YD
D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D002455:Cell Division; D003470:Culture Media; D003593:Cytoplasm / Q000737:chemistry / Q000201:enzymology; D007124:Immunoenzyme Techniques; D000432:Methanol / Q000378:metabolism*; D008772:Methylococcaceae / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism
D003470:Culture Media; C020145:formaldehyde dehydrogenase (glutathione); D000445:Aldehyde Oxidoreductases; D000432:Methanol