Use of ethanol-eluted hydrophobic interaction chromatography in the purification of serum amyloid A.
A two-step procedure for the purification of the acute-phase reactant serum amyloid A from serum is described. A hydrophobic interaction chromatography medium, octyl-Sepharose CL4B, eluted with increasing concentrations of EtOH was used as the first step in the purification. The concentrate from this step was applied to a gel filtration column of Sephacryl S-200 and eluted with 10% formic acid. The overall recovery of purified serum amyloid A from serum was 56%. This represents the first time that serum amyloid A has been purified without the use of high concentrations of guanidine or urea. The method presented could easily be scaled up to allow the purification of large quantities of serum amyloid A or readily adapted to the purification of other serum apolipoproteins.
Smith
J W
JW
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-6495.
McDonald
T L
TL
D000210:Acute-Phase Reaction / Q000097:blood; D000595:Amino Acid Sequence; D002847:Chromatography, Agarose / Q000379:methods*; D002850:Chromatography, Gel; D000431:Ethanol; D006801:Humans; D008969:Molecular Sequence Data; D012685:Sepharose / Q000031:analogs & derivatives; D000685:Serum Amyloid A Protein / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*
D000685:Serum Amyloid A Protein; D000431:Ethanol; C027176:octyl-sepharose CL-4B; D012685:Sepharose