New approaches to peptide synthesis with the help of trypsin.
Picolyl esters of amino acids are suitable inverse substrates for trypsin. These esters can be used for peptide synthesis catalyzed by trypsin. Study of hydrolysis of non-specific substrates for trypsin permits to conclude, that hydrogen band in P'2 subsite is very important for their reactivity. Carboxamido methyl esters (CAM-esters) are the most suitable substrates for trypsin catalyzed peptide synthesis. Using CAM-esters and trypsin several di-,tri- and tetra-peptides were synthesized with good yields.
Mitin YuV
Institute of Protein Research, Academy of Sciences, Puschino, Moscow Region, USSR.
Zapevalova
N P
NP
Gorbunova EYu
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D004151:Dipeptides / Q000138:chemical synthesis / Q000737:chemistry; D004952:Esters / Q000737:chemistry; D006868:Hydrolysis; D007700:Kinetics; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D010848:Picolinic Acids / Q000737:chemistry; D013379:Substrate Specificity; D014357:Trypsin / Q000737:chemistry*
D000596:Amino Acids; D004151:Dipeptides; D004952:Esters; D010455:Peptides; D010848:Picolinic Acids; D014357:Trypsin