Alkaline phosphatase from Echinococcus multilocularis: purification and characterization.
1. Kinetic and physical parameters of purified alkaline phosphatase from Echinococcus multilocularis metacestodes, livers of infected gerbils and control animals were determined. 2. Km value for p-nitrophenyl phosphate was about 0.05 +/- 0.02 mM for the three enzymes. 3. Vmax values were 357 +/- 67 nmol/min/mg proteins for metacestode enzyme, and 6.7 +/- 1.1 and 6.7 +/- 0.8 nmol/min/mg proteins for liver enzyme of infected and control animals, respectively. 4. Mr and pI were different for the parasite and hepatic enzyme. 5. The parasite enzyme was less sensitive to the elevation of temperature than hepatic enzyme. 6. The isatin inhibition was a competitive inhibition type for parasite and uncompetitive type for host liver enzyme.
Sarciron
M E
ME
Département de Parasitologie et de Pathologie Exotique, Université Claude-Bernard, Lyon, France.
Hamoud
W
W
Azzar
G
G
Petavy
A F
AF
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002846:Chromatography, Affinity; D004446:Echinococcus / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005849:Gerbillinae; D006863:Hydrogen-Ion Concentration; D007510:Isatin / Q000494:pharmacology; D007525:Isoelectric Focusing; D007700:Kinetics; D008099:Liver / Q000201:enzymology / Q000469:parasitology; D009596:Nitrophenols / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D013696:Temperature
D009596:Nitrophenols; D009943:Organophosphorus Compounds; C008644:nitrophenylphosphate; D007510:Isatin; D000469:Alkaline Phosphatase