Sugar chain heterogeneity of bone and liver alkaline phosphatase in serum.
Fractionation of bone and liver alkaline phosphatase (EC 3.1.3.1; ALP) in serum by serial lectin affinity chromatography has demonstrated differences in the sugar chain structure of bone and liver ALP in serum from that previously reported in the corresponding tissues, with a lower content of high mannose or hybrid-type sugar chains and a higher content of biantennary complex-type chains. Furthermore, the bone and liver ALPs were found to differ in the latter with the bone fraction showing a greater content of fucose residues.
Kuwana
T
T
Department of Laboratory Medicine, Niigata University School of Medicine, Japan.
Sugita
O
O
Yakata
M
M
D000328:Adult; D000469:Alkaline Phosphatase / Q000097:blood / Q000737:chemistry*; D001842:Bone and Bones / Q000201:enzymology; D002648:Child; D002675:Child, Preschool; D002846:Chromatography, Affinity; D006023:Glycoproteins / Q000737:chemistry*; D006801:Humans; D007527:Isoenzymes / Q000097:blood / Q000737:chemistry*; D037102:Lectins; D008099:Liver / Q000201:enzymology
D006023:Glycoproteins; D007527:Isoenzymes; D037102:Lectins; D000469:Alkaline Phosphatase