title: High-performance liquid chromatographic analysis of oxytetracycline in chinook salmon following administration of medicated feed.
abstract: A high-performance liquid chromatographic assay was developed to detect oxytetracycline (OTC) in chinook salmon muscle tissue. A solid-phase extraction protocol was used to recover OTC and the internal standard, epitetracycline hydrochloride, from the salmon tissue samples. OTC was analyzed using a mobile phase of methanol-0.02 M phosphate buffer, pH 2.25 (60:190), an ultraviolet detection wavelength of 365 nm and 250 mm x 4.6 mm I.D. Ultrasphere ODS column. A linear calibration curve (r2 = 0.999) of OTC in salmon muscle tissue from 0.05 to 3.0 ppm was obtained. Using a signal-to-noise ratio of 5:1, the OTC detection limit was 0.5 ppm in salmon muscle tissue. OTC recovery (74.4%) and intra-assay variability (2.3%) were optimized for salmon muscle tissue. An in vivo feeding study was performed by administrating OTC-medicated feed for a period of 10 days, followed by a 42-day sampling period. The half-life for the elimination of OTC in chinook salmon muscle tissue was found to be 5.4 days.
authors.affiliation: Faculty of Pharmaceutical Sciences, Vancouver, B.C., Canada.
authors.forename: R G
authors.initials: RG
authors.lastname: Aoyama
authors.forename: K M
authors.initials: KM
authors.lastname: McErlane
authors.forename: H
authors.initials: H
authors.lastname: Erber
authors.forename: D D
authors.initials: DD
authors.lastname: Kitts
authors.forename: H M
authors.initials: HM
authors.lastname: Burt
mesh_terms: D000821:Animal Feed; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D007700:Kinetics; D009132:Muscles / Q000737:chemistry*; D010118:Oxytetracycline / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D012474:Salmon
chemical_list: D010118:Oxytetracycline