title: [Evaluation of the activity of cytokine genes--a new diagnostic test in immunology].
abstract: The increased level of IL-1 mRNA was discovered in neutrophils of patients suffering from pollinosis for a long time and responding poorly to specific immunotherapy. Moreover, neutrophils from patients with exacerbation of pollinosis responded to stimuli less than cells from healthy donors. In remission, the activated neutrophils from pollinosis patients and healthy donors had the same levels of IL-1b mRNA. Tumor necrosis factor alpha expression was unchanged in neutrophils of pollinosis patients. Changes in the status of cells from patients were detected with a simple procedure of dot hybridization. Peripheral blood cells were used as available material. This allows one to hope that the approach can be used for analyzing the patients' immune status in choosing the treatment course as well as for controlling the treatment effect.
authors.forename: O A
authors.initials: OA
authors.lastname: Kupriianova
authors.forename: V A
authors.initials: VA
authors.lastname: Mordvinov
authors.forename: I P
authors.initials: IP
authors.lastname: Ivanova
authors.forename: O L
authors.initials: OL
authors.lastname: Krugleeva
authors.forename: E M
authors.initials: EM
authors.lastname: Tananko
authors.forename: E A
authors.initials: EA
authors.lastname: Braga
authors.forename: V V
authors.initials: VV
authors.lastname: Nosikov
authors.forename: V A
authors.initials: VA
authors.lastname: Kozlov
authors.forename: V P
authors.initials: VP
authors.lastname: Lozovoĭ
mesh_terms: D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis / Q000097:blood / Q000235:genetics*; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D009693:Nucleic Acid Hybridization / Q000502:physiology*; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D006255:Rhinitis, Allergic, Seasonal / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology
chemical_list: D007375:Interleukin-1; D015347:RNA Probes; D012333:RNA, Messenger
vernacular_title: Otsenka aktivnosti genov tsitokinov--novyĭ diagnosticheskiĭ test v immunologii.