Simple and fast chromatographic method for the simultaneous determination of vanillylmandelic acid and homovanillic acid in human urine.
A high-performance liquid chromatographic method for the determination of vanillylmandelic acid and homovanillic acid is described. The method is fast despite the great polarity differences between the two acids. Moreover the sample pretreatment is quick and it does not need complex or expensive equipment. The only requirement is the disposition of two pumps (or at least two eluent reservoirs) operated alternatively by means of a switching valve placed before the injection device. This makes the method available for most routine laboratories.
Zilli
M A
MA
Laboratorio Analisi Cliniche, O.C. S. Maria Della Misericordia, Udine, Italy.
D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006719:Homovanillic Acid / Q000652:urine*; D006801:Humans; D015203:Reproducibility of Results; D014642:Vanilmandelic Acid / Q000652:urine*
D014642:Vanilmandelic Acid; D006719:Homovanillic Acid