Effects of oxidants on protein breakdown in skeletal muscle.
Oxidants have been found to increase protein breakdown in erythroid cells. In skeletal muscle, phenylhydrazine decreased by 24-28% rates of protein degradation. Both H2O2 (5 mM) and glucose oxidase (0.2 U/ml) decreased by 82-88% rates of proteolysis only in skeletal muscles pretreated with sodium azide (0.25 mM) to inactivate endogenous catalase. Thus, the effect of various oxidants on protein breakdown may differ depending on the cell or tissue type.
Gecha
O M
OM
Department of Animal Sciences, Rutgers University, New Brunswick, NJ 08903.
Culbert
L A
LA
Fagan
J M
JM
D000818:Animals; D001386:Azides / Q000494:pharmacology; D002374:Catalase / Q000037:antagonists & inhibitors / Q000378:metabolism; D005949:Glucose Oxidase / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008297:Male; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology*; D010659:Phenylhydrazines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D019810:Sodium Azide
D001386:Azides; D009124:Muscle Proteins; D016877:Oxidants; D010659:Phenylhydrazines; C030299:phenylhydrazine; D019810:Sodium Azide; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D002374:Catalase