title: Enzyme immunoassay to determine serum myoglobin in patients with acute myocardial infarction.
abstract: A method of "sandwich" enzyme immunoassay was developed for determination of human serum myoglobin with the use of myoglobin isolated from human myocardium and gammaglobulin fraction of a specific sheep antiserum labelled with horseradish peroxidase. The linear part of the calibration curve within the range of 0.08-2.2 nmol/l is suitable for accurate quantitative reading of myoglobin concentration. Intra- and interassay variation coefficients are 7% and 11.2%, respectively. A comparison of 100 serum samples assessed by means of commercially available RIA kit and by the given method revealed a correlation coefficient of 0.86.
authors.affiliation: Department of Medical Chemistry, Charles University Medical School, Prague, Czechoslovakia.
authors.forename: I
authors.initials: I
authors.lastname: Kurcová
authors.forename: H
authors.initials: H
authors.lastname: Tlaskalová
authors.forename: M
authors.initials: M
authors.lastname: Srámek
authors.forename: J
authors.initials: J
authors.lastname: Bouzek
authors.forename: P
authors.initials: P
authors.lastname: Mancal
authors.forename: I
authors.initials: I
authors.lastname: Malbohan
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D005500:Follow-Up Studies; D006801:Humans; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis; D009206:Myocardium / Q000378:metabolism; D009211:Myoglobin / Q000097:blood*; D012016:Reference Values
chemical_list: D009211:Myoglobin