Platelet-activating factor (PAF) induces contraction of saponin-skinned smooth muscle of coronary artery.
The effect of platelet-activating factor (PAF) on the development of isometric tension by saponin-skinned coronary artery was studied. PAF caused two types of contractions of coronary smooth muscle cells (SMC): (i) rapid, transient (phasic) contractions of SMC were induced by inositol 1,4,5-trisphosphate dependent Ca2+ release from sarcoplasmic reticulum (SR); (ii) slow sustained (tonic) contractions were induced by increase in Ca(2+)-sensitivity of the contractile apparatus of SMC by protein kinase C activation. The present results support the hypothesis that, in SMC of coronary artery, PAF receptors are located not only on the plasma membrane, but also on the SR membranes.
Soloviev
A I
AI
A.A. Bogomoletz Institute of Physiology, Kiev, Ukraine, U.S.S.R.
Braquet
P
P
D000818:Animals; D002118:Calcium / Q000494:pharmacology / Q000502:physiology; D003331:Coronary Vessels / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000187:drug effects; D009119:Muscle Contraction / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology*; D010972:Platelet Activating Factor / Q000494:pharmacology*; D012503:Saponins; D012519:Sarcoplasmic Reticulum / Q000502:physiology; D013552:Swine
D010972:Platelet Activating Factor; D012503:Saponins; D002118:Calcium