title: Analysis of the role of 5' regulatory mutations in the activation of quiescent metallothionein genes after carcinogen treatment.
abstract: S49 are mouse thymic lymphoma cells which do not express the two closely linked mouse metallothionein (MT) genes; however, previous studies demonstrated that treatment of S49 cells with chemical carcinogens or ultraviolet irradiation can activate these quiescent genes. To determine if activation of MT-I or MT-II in these variants is a result of cis-acting mutations, we amplified and sequenced the immediate 5' regions of 19 cadmium resistant S49 variants: MT-I+/MT-II-, MT-I-/MT-II+, MT-I+/MT-II+, and MT-I-/MT-II-. None of the variants contained mutations in the analyzed regions. Thus, the observed changes in MT expression must result from mutations at other sites or from non-mutational mechanisms.
authors.affiliation: Department of Pathology, Baylor College of Medicine, Houston, Texas 77030.
authors.forename: E
authors.initials: E
authors.lastname: Aguilar-Cordova
authors.forename: R M
authors.initials: RM
authors.lastname: Lebovitz
authors.forename: M W
authors.initials: MW
authors.lastname: Lieberman
mesh_terms: D000818:Animals; D001483:Base Sequence; D002273:Carcinogens / Q000494:pharmacology; D008668:Metallothionein / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction; D012045:Regulatory Sequences, Nucleic Acid; D014407:Tumor Cells, Cultured
chemical_list: D002273:Carcinogens; D008668:Metallothionein