title: Participation of membrane skeleton proteins in aggregation of epidermal growth factor receptors in A431 cells.
abstract: Polyclonal antibody against alpha-spectrin of chicken erythrocytes was prepared. This antibody as well as anti-vinculin and anti-annexin I and II, were used for localization of the antigens in A431 cells during translocation of epidermal growth factor receptors (EGF-Rs) on cell surface. During aggregation of EGF-Rs only spectrin and actin aggregates colocalized with the "capped" receptors in adherent as well as in suspended cells. Physiological implication of spectrin involvement in EGF-Rs redistribution in A431 cells is discussed.
authors.affiliation: Nencki Institute of Experimental Biology, Department of Cell Biology, Warsaw, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Kwiatkowska
authors.forename: I A
authors.initials: IA
authors.lastname: Khrebtukova
authors.forename: A
authors.initials: A
authors.lastname: Sobota
mesh_terms: D000199:Actins / Q000502:physiology*; D000818:Animals; D000906:Antibodies / Q000032:analysis; D002478:Cells, Cultured; D002645:Chickens; D004817:Epidermis; D066246:ErbB Receptors / Q000502:physiology*; D004910:Erythrocyte Membrane; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D011940:Receptor Aggregation / Q000502:physiology*; D013049:Spectrin / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D000199:Actins; D000906:Antibodies; D008565:Membrane Proteins; D013049:Spectrin; D066246:ErbB Receptors