The effects of glutathione depletion on the biodistribution of Cu(PTSM) in rats.
The tissue retention of radiocopper afforded by intravenous injection of the proposed blood flow imaging agent, 62Cu-labeled copper(II) pyruvaldehyde bis(N4-methylthiosemicarbazone) [Cu(PTSM)], is thought to result from reductive decomposition of the copper(II) complex by intracellular sulfhydryls (e.g. glutathione, GSH). To determine if the tissue uptake and retention of this tracer adequately measures perfusion in tissues containing altered GSH concentrations, the biodistribution of copper-67 labeled Cu(PTSM) was determined in GSH-depleted rats. Despite treatment to induce relatively large reductions in tissue GSH levels, it was found that only very small changes in the biodistribution of copper-labeled Cu(PTSM) occurred in the treated rats compared to untreated controls.
Barnhart-Bott
A
A
Department of Medicinal Chemistry and Pharmacognosy, Purdue University, West Lafayette, IN 47907.
Green
M A
MA
D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D019328:Buthionine Sulfoximine; D003301:Copper Radioisotopes; D005978:Glutathione / Q000172:deficiency*; D007457:Iodine Radioisotopes; D008297:Male; D008298:Maleates / Q000494:pharmacology; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D009942:Organometallic Compounds / Q000493:pharmacokinetics*; D011837:Radiation-Protective Agents / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013882:Thiosemicarbazones / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D003301:Copper Radioisotopes; D007457:Iodine Radioisotopes; D008298:Maleates; D009942:Organometallic Compounds; D011837:Radiation-Protective Agents; D013882:Thiosemicarbazones; D008717:Methionine Sulfoximine; C052629:copper pyruvaldehyde bis(N(4)-methylthiosemicarbazone) complex; D019328:Buthionine Sulfoximine; C014476:diethyl maleate; D005978:Glutathione; D000983:Antipyrine; C008135:iodoantipyrine