Sono-enzymatic peptide synthesis in organic solvent.
Copolymerized compounds of acrylated derivatives of alpha-chymotrypsin and polyethylene glycol (P.E.G.) have been prepared and used as biocatalysts for peptide synthesis in organic solvent containing low quantity of water. In order to increase the velocity of the coupling reactions, without loss of enzyme activity, we have used the principle of sonication. Some results and future projects are presented.
Fulcrand-Rolland
V
V
Laboratoire des Aminoacides et Peptides, URA-CNRS n. 468 Université Montpellier II, France.
Duc Hua
T
T
Lazaro
R
R
Viallefont
P
P
D000595:Amino Acid Sequence; D002384:Catalysis; D002918:Chymotrypsin / Q000737:chemistry; D004151:Dipeptides / Q000138:chemical synthesis / Q000737:chemistry; D004800:Enzymes, Immobilized; D007700:Kinetics; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D011092:Polyethylene Glycols; D012997:Solvents; D013010:Sonication
D004151:Dipeptides; D004800:Enzymes, Immobilized; D010455:Peptides; D012997:Solvents; D011092:Polyethylene Glycols; D002918:Chymotrypsin