[A comparison of 2 T-lymphoid cell lines persistently infected with the human immunodeficiency virus (HIV-1) with different productive capacities].
T-lymphoid cell lines (H9/CBL-4 and CEM/CBL-4) persistently infected with HIV-1 were observed simultaneously for 6.5 months. The virus activity was characterized by such parameters as the number of infected cells determined by fluorescent antibody technique, the total level of virus--specific protein synthesis determined by immune blotting method, and the capacity to infect H9 and CEM cells. A comparative analysis of the two cell lines helped define the evaluation criteria for high and low productivity cultures. It was shown that a short-term virus persistence could exist in high-productivity cultures and long-term persistence in low-productivity cultures. The cytopathic activity of virus in cultures could be judged by accumulation of virus protein p24 in cell-free supernatants, this being one of the factors defining the efficacy of infection of H9 and CEM T-lymphoid cells.
Selimova
L M
LM
Kliushnik
S Iu
SIu
D002460:Cell Line; D002478:Cells, Cultured / Q000382:microbiology; D003588:Cytopathogenic Effect, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D016655:HIV Core Protein p24 / Q000032:analysis; D015497:HIV-1 / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D015151:Immunoblotting; D013601:T-Lymphocytes / Q000382:microbiology*; D013997:Time Factors; D014776:Virus Cultivation / Q000379:methods
D016655:HIV Core Protein p24
Sravnenie dvukh T-limfoidnykh kletochnykh liniĭ, persistentno infitsirovannykh virusom immunodefitsita cheloveka (VICh-1), s razlichnoĭ produktivnoĭ sposobnost'iu.