Determination of bioactive peptides using capillary zone electrophoresis/mass spectrometry.
Mixtures of bioactive peptides have been analyzed by capillary zone electrophoresis/mass spectrometry (CZE/MS) using an on-line coaxial continuous-flow fast atom bombardment interface. High separation efficiencies (up to 410,000 theoretical plates) were obtained from low femtomole levels of peptides. The analysis of basic peptides was accomplished by using aminopropyl-silylated CZE columns to minimize zone broadening due to adsorption effects. CZE/MS/MS data were acquired from femtomole levels of peptides in electrophoretic real time.
Moseley
M A
MA
Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Deterding
L J
LJ
Tomer
K B
KB
Jorgenson
J W
JW
D000042:Absorption; D000595:Amino Acid Sequence; D002630:Chemotactic Factors / Q000032:analysis*; D004586:Electrophoresis / Q000379:methods*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000032:analysis*; D009842:Oligopeptides / Q000032:analysis*; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods*
D002630:Chemotactic Factors; D009479:Neuropeptides; D009842:Oligopeptides