title: Detection of blood stage antigens of Plasmodium vivax by sandwich ELISA using pan-species monoclonal antibodies and polyclonal antibodies.
abstract: This paper reports an improved PcAb-McAb-ELISA test to detect blood stage Plasmodium vivax antigen in which the plates were coated with rabbit anti-P. cynomolgi polyclonal antibody to capture the antigens in test samples and two monoclonal antibodies, M26-32 and 3F9, were added together to react with the captured antigens. The coincidence rate with this test was 93% with microscopically confirmed P. vivax cases, 97% with normal samples, 95% with microscopically negative fever cases from nonendemic areas and 86% from endemic areas, respectively. The sensitivity was greater than 1 parasite/10(5) RBC.
authors.affiliation: Jiangsu Provincial Institute of Parasitic Diseases, Wuxi, People's Republic of China.
authors.forename: Q
authors.initials: Q
authors.lastname: Gao
authors.forename: C X
authors.initials: CX
authors.lastname: Yang
authors.forename: S Y
authors.initials: SY
authors.lastname: Zhang
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Yang
authors.forename: W Q
authors.initials: WQ
authors.lastname: Zhang
authors.forename: J L
authors.initials: JL
authors.lastname: Li
mesh_terms: D000906:Antibodies; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards*; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005189:False Positive Reactions; D006801:Humans; D016780:Malaria, Vivax / Q000097:blood / Q000453:epidemiology* / Q000276:immunology; D008853:Microscopy; D010966:Plasmodium vivax / Q000276:immunology*; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan