Presynaptic alpha-latrotoxin receptor components interact with protein p65 of synaptic vesicle membranes.
Purified preparations of bovine brain alpha-latrotoxin receptor contain proteins of 39 kDa and 65 kDa. Sequence analysis shows that the 65 kDa protein corresponds to p65, a synaptic vesicle membrane protein previously identified in rat synaptic vesicles, and that the 39 kDa protein is a proteolytic fragment of the 65 kDa protein. The 39 kDa and 65 kDa proteins appear in receptor samples because of their specific interaction with components of the alpha-latrotoxin receptor. This interaction may represent an essential step in perfusion and/or the fusion of synaptic vesicle membranes with the plasma membrane of the presynaptic terminal, both of which are final steps in the exocytosis of neurotransmitters.
Surkova
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Shemyakin Institute of Biorganic Chemistry, Academy of Sciences of the USSR, Moscow.
Grishin
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D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins; D002417:Cattle; D008561:Membrane Fusion; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000276:immunology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000276:immunology; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D011950:Receptors, Cholinergic / Q000737:chemistry* / Q000276:immunology; D018000:Receptors, Peptide; D013111:Spider Venoms / Q000737:chemistry*; D013570:Synaptic Membranes / Q000737:chemistry*; D013572:Synaptic Vesicles / Q000737:chemistry*; D050863:Synaptotagmin I; D050857:Synaptotagmins
D002135:Calcium-Binding Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D011950:Receptors, Cholinergic; D018000:Receptors, Peptide; D013111:Spider Venoms; D050863:Synaptotagmin I; C034800:alpha-latrotoxin receptor; D050857:Synaptotagmins