Changes in cellular dry mass during macrophage development.
Measurements of dry mass were made on developing human macrophages in suspension culture using a scanning and integrating microinterferometer. The mean dry mass (+/- SEM) of day 0 monocytes of 19 healthy individuals was 53.6 +/- 2.4 pg. There was a significant increase in cellular dry mass over the period of culture, mean values at 2, 4 and 6 days being 61.1, 79.8 and 110.4 pg, respectively. A comparison of the results with those obtained previously by ultrastructural morphometry inferred that there is a disproportionate increase in the water content of macrophages during development. The technique may have potential for investigating changes in mononuclear phagocytes in clinical states.
Sokol
R J
RJ
Department of Haematology, University of Sheffield, UK.
Wales
J
J
Hudson
G
G
Goldstein
D J
DJ
James
N T
NT
D000328:Adult; D001834:Body Water / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008264:Macrophages / Q000737:chemistry*; D008297:Male; D008857:Microscopy, Interference; D008875:Middle Aged