The influence of experimentally produced oligohydramnios on lung growth and pulmonary surfactant content in fetal rabbits.
To study the effect of oligohydramnios on lung growth and biochemical lung development in fetal rabbits, amniotic fluid was drained through a tube inserted into the maternal peritoneal cavity on the 23 day of gestation. Littermate fetuses without an amniotic shunt were used as controls. The fetuses were delivered abdominally on the 28 day of gestation. In a total of 8 pregnant does, 17 fetuses underwent amniotic shunting and 22 fetuses were used as controls. The amniotic shunt produced a significant reduction in the amniotic fluid volume. There were no differences in the wet weights of the fetal body, liver or brain between the two groups. However, the amniotic shunt significantly decreased the wet weight of the fetal lung, fetal lung wet weight/body weight ratio, and protein concentration per lung as compared to the control fetuses. In the fetal liver and brain tissues, no changes were found in the concentrations of total phospholipids, phosphatidylcholine (PC) or disaturated phosphatidylcholine (DSPC, the main component of lung surfactant) per g of wet tissue and per mg of protein. However, the lungs of the fetuses with amniotic shunts contained significantly more PC and DSPC, and the L/S ratio was higher than in the control fetuses. These results suggest that the oligohydramnios produced by an amniotic shunt causes pulmonary hypoplasia, but raises the pulmonary surfactant content of fetal rabbit lung.
Higuchi
M
M
Department of Obstetrics and Gynecology, Akita University School of Medicine, Japan.
Kato
T
T
Yoshino
H
H
Matsuda
K
K
Gotoh
K
K
Hirano
H
H
Koyama
K
K
Maki
M
M
D000818:Animals; D001835:Body Weight; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism; D004195:Disease Models, Animal; D037102:Lectins / Q000032:analysis; D008099:Liver / Q000033:anatomy & histology / Q000378:metabolism; D008168:Lung / Q000033:anatomy & histology / Q000196:embryology* / Q000378:metabolism*; D016104:Oligohydramnios / Q000503:physiopathology*; D009929:Organ Size; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D011663:Pulmonary Surfactants / Q000096:biosynthesis*; D011817:Rabbits; D013109:Sphingomyelins / Q000378:metabolism
D037102:Lectins; D010713:Phosphatidylcholines; D010743:Phospholipids; D011506:Proteins; D011663:Pulmonary Surfactants; D013109:Sphingomyelins