Monocyte adhesion to human saphenous vein in vitro.
Monocyte adhesion to endothelium is believed to be an initiating event in atherosclerosis both in arteries and in saphenous vein coronary artery bypass grafts. We have developed a method to quantify adhesion of 51Cr-labelled human blood monocytes to saphenous vein. Adhesion to the intimal surface was measured to uniform 6 mm diameter discs, the adventitia of which was embedded in a layer of inert silicone grease. The identity, number and location of bound cells was further defined by scanning electron microscopy. The proportion of monocytes adhering to discs of freshly-isolated vein was 7.1 +/- 1.2% (SE, n = 8), which was equivalent to 500 +/- 80 monocytes/mm2. The percentage of monocytes adhering was independent of the number of monocytes added in the range 5-50 x 10(4). Scanning micrographs showed 80% endothelial coverage with monocytes adhering preferentially but not exclusively to subendothelium. Endothelial removal increased monocyte adhesion by 1.60 +/- 0.15-fold (n = 8, P less than 0.01). Vein surgically prepared for use in coronary bypass surgery, had a 50% reduction in endothelial coverage and a small but non significant (1.14 +/- 0.13-fold, n = 8) increase in monocyte adhesion. Treatment of freshly-isolated vein for 4 h at 37 degrees C with 1 micrograms/ml of E. Coli endotoxin followed by extensive washing did not remove endothelium but increased monocyte adherence by 1.46 +/- 0.18-fold (n = 8, P less than 0.05). The proportion of monocytes adhering to veins which had been cultured for 14 days was similar to freshly isolated veins (6.4 +/- 0.8%, n = 7), but in cultured veins, monocytes adhered preferentially to cells with typical endothelial morphology. Endotoxin treatment of cultured freshly-isolated veins increased monocyte adhesion by 1.77 +/- 0.23-fold (n = 8, P less than 0.05). The data show that both endothelial activation, and exposure of subendothelium promote monocyte adhesion to human saphenous vein.
Cooper
J P
JP
Department of Cardiology, University of Wales College of Medicine, Heath Park, Cardiff, U.K.
Newby
A C
AC
D002448:Cell Adhesion; D002455:Cell Division; D002860:Chromium Radioisotopes; D001026:Coronary Artery Bypass; D046508:Culture Techniques; D004730:Endothelium, Vascular / Q000166:cytology / Q000648:ultrastructure; D004731:Endotoxins / Q000494:pharmacology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009000:Monocytes / Q000502:physiology* / Q000648:ultrastructure; D012501:Saphenous Vein / Q000166:cytology* / Q000637:transplantation
D002860:Chromium Radioisotopes; D004731:Endotoxins; C044101:endotoxin, Escherichia coli