Two-dimensional mapping by high-performance liquid chromatography of pyridylamino oligosaccharides from various glycosphingolipids.
A method to map sugars two-dimensionally for the analysis of the structures of oligosaccharides from glycosphingolipids is described. Nine neutral and ten acidic oligosaccharides were obtained from glycosphingolipids by endoglycoceramidase digestion and labelled with 2-aminopyridine. The pyridylamino oligosaccharides were clearly separated by high-performance liquid chromatography on commercially available C18-silica and amide-silica column. All compounds tested were mapped without any overlapping. The separation of the pyridylamino oligosaccharides on the C18-silica column depended on the numbers and positions of sialic acid and N-acetylhexosamine residues; on the amide-silica column, the separation depended on the total number of sugar residues.
Ohara
K
K
Biotechnology Research Laboratories, Takuro Shuzo Co., Ltd., Shiga, Japan.
Sano
M
M
Kondo
A
A
Kato
I
I
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006028:Glycosphingolipids / Q000032:analysis*; D006595:Hexosamines / Q000032:analysis; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D012794:Sialic Acids / Q000032:analysis
D006028:Glycosphingolipids; D006595:Hexosamines; D009844:Oligosaccharides; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid