Lack of evidence for a saturable tetracycline transport system in Staphylococcus aureus.
Previous studies on tetracycline transport into Staphylococcus aureus identified a high-affinity, saturable uptake system for the antibiotic (Km, 4.76 microM) (B.L. Hutchings, Biochim. Biophys. Acta 174:734-738, 1969). However, the earlier results could not be confirmed using conditions that permitted energy-dependent, concentrative uptake of tetracycline. Kinetic artifacts introduced by inappropriate washing procedures may explain the previous results.
Chopra
I
I
Department of Microbial Biochemistry and Genetics, Lederle Laboratories, American Cyanamid, Pearl River, New York 10965.
Ismail
S
S
Oliva
B
B
D001693:Biological Transport, Active; D002478:Cells, Cultured; D004926:Escherichia coli; D013211:Staphylococcus aureus / Q000378:metabolism*; D013752:Tetracycline / Q000378:metabolism*
D013752:Tetracycline