Assessment of a selected method for creatinine with special emphasis on bilirubin interference.
An HPLC-based method for the determination of creatinine was evaluated, with emphasis on interference by bilirubin. The method differed from other procedures, in that deproteinization was by ultrafiltration instead of precipitation with trichloroacetic acid. This allows a rapid, direct analysis by HPLC with very few experimental steps. Analytical results showed linearity up to 5000 mumol/l and an average analytical recovery of 100%. Within-day imprecision was 0.8-1.4%, and day-to-day imprecision was 0.5-2.4%.
Zwang
L
L
Department of Clinical Chemistry, Academic Hospital Rotterdam-Dijkzigt, The Netherlands.
Blijenberg
B G
BG
D001663:Bilirubin / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003401:Creatine / Q000097:blood*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D003401:Creatine; D001663:Bilirubin