[A whole-blood micromethod to determine lymphocyte subsets in capillary blood, comparison with other methods of indirect immunofluorescence].
Six lymphocyte antigens were detected with monoclonal antibodies in 0.2 ml capillary blood by a whole blood micromethod. After the lysis of erythrocytes of one drop of blood the remaining cells were layered on slides and were stained by indirect immunofluorescence. The relative and absolute counts of the lymphocyte subsets were of normal value and did not differ from the counts of flow cytometry and of slide and tube tests.
Schäffner
G
G
Staatliches Kontrollinstitut für immunbiologische Arzneimittel, Humboldt-Universität zu Berlin.
Seeger
E
E
Volk
H D
HD
Fischer
R
R
Kiowski
S
S
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D002196:Capillaries; D005455:Fluorescent Antibody Technique; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D016131:Lymphocyte Subsets / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD
Eine Vollblut-Mikromethode zum Nachweis von Lymphozytensubpopulationen im Kapillarblut, Vergleich mit anderen Methoden der indirekten Immunfluoreszenz.