title: Pepsin behavior as a catalyst in equilibrium-controlled peptide synthesis.
abstract: It has been shown that in the course of equilibrium peptide synthesis pepsin gradually disappeared from the liquid phase due to its entrapment within a gel formed by the hexapeptide product, while retaining its activity. The inclusion into the precipitate was not specific for pepsin so far as inert proteins-lysozyme, ribonuclease A and carbonic anhydrase, when added to the reaction mixture, became also co-precipitated with the hexapeptide formed. It appears that co-precipitation of pepsin-an important factor limiting the enzyme efficiency, might be operative as well for other proteinases used to catalyze peptide synthesis.
authors.affiliation: Chemistry Department of Moscow State University, USSR.
authors.lastname: Filippova IYu
authors.forename: E N
authors.initials: EN
authors.lastname: Lysogorskaya
authors.forename: V V
authors.initials: VV
authors.lastname: Anisimova
authors.forename: C A
authors.initials: CA
authors.lastname: Abdel Malak
authors.forename: G I
authors.initials: GI
authors.lastname: Lavrenova
authors.forename: V M
authors.initials: VM
authors.lastname: Stepanov
mesh_terms: D000595:Amino Acid Sequence; D002384:Catalysis; D007700:Kinetics; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry; D010434:Pepsin A / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D009842:Oligopeptides; D010455:Peptides; D010434:Pepsin A