[Separation of epimers of anisodamine (654-2) with HPLC using chiral additives in the mobile phase].
The separation of epimers of anisodamine were studied with HPLC using a reversed phase ODS C18 column. Several chiral additives were tried in the mobile phases. d-Camphorsulfonic acid and L-(+)-dibutyl tartrate showed good resolution ability for the separation of the isomers, a pair of epimers of anisodamine were well separated; while beta-cyclodextrin appeared to have no selectivity for the epimers.
Xie
L
L
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Chi
H
H
Zhou
T H
TH
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D012991:Solanaceous Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism
D012991:Solanaceous Alkaloids; C003922:anisodamine