Determination of cocaine, benzoylecgonine and ecgonine methyl ester in plasma by reversed-phase high-performance liquid chromatography.
A combined assay is described for cocaine and its major metabolites, benzoylecgonine and ecgonine methyl ester. The method uses electrochemical and ultraviolet detectors in series. A non-silica column is used with high-pH mobile phase. The three compounds are completely separated from other cocaine metabolites. The assay has been suitable for pharmacokinetic studies of cocaine disposition in animal studies.
Miller
R L
RL
Department of Pharmacy Practice, College of Pharmacy, University of Florida, Gainesville 32610.
DeVane
C L
CL
D000818:Animals; D003042:Cocaine / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D004563:Electrochemistry; D005260:Female; D006863:Hydrogen-Ion Concentration; D012756:Sheep; D013056:Spectrophotometry, Ultraviolet
C005618:benzoylecgonine; D003042:Cocaine; C034524:ecgonine methyl ester