title: Production of platelet-activating factor by human normodense and hypodense eosinophils.
abstract: Normodense eosinophils and neutrophils from normal donors produced considerable amounts of platelet-activating factor (PAF) when stimulated with ionophore A23187. PAF produced by eosinophils appeared to be degraded more rapidly than PAF formed by neutrophils, suggesting a higher activity of PAF-degrading enzyme in eosinophils. Substantial proportions of PAF newly formed by both eosinophils and neutrophils were shown to be cell-associated. By comparison, hypodense eosinophils obtained from a patient with idiopathic hypereosinophilic syndrome produced an extremely large amount of PAF and released much of it into the incubation medium. The accelerated formation of PAF in hypodense eosinophils may be related to various cardiovascular complications associated with hypereosinophilic syndrome.
authors.affiliation: Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Ojima-Uchiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Masuzawa
authors.forename: T
authors.initials: T
authors.lastname: Sugiura
authors.forename: K
authors.initials: K
authors.lastname: Waku
authors.forename: T
authors.initials: T
authors.lastname: Fukuda
authors.forename: S
authors.initials: S
authors.lastname: Makino
mesh_terms: D004802:Eosinophilia / Q000097:blood; D004804:Eosinophils / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009504:Neutrophils / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000096:biosynthesis*; D012016:Reference Values; D013577:Syndrome; D013997:Time Factors
chemical_list: D010972:Platelet Activating Factor