[Studies on double-labelling method for determination of T lymphocyte subpopulations].
In order to determine the concurrent expression of DR and CD antigen of T lymphocyte surface markers, the fluorescence-rosette double-labelling technique was developed by employing monoclonal antibody against HLA-DR and sensitized sheep erythrocytes with OKT series of monoclonal antibodies against cell surface antigen. Its reliability was evaluated by comparison with two-step isolating technique for determining DR antigen of activated T lymphocyte subsets in 30 healthy volunteers. The result showed there was no statistic difference between results determined with two methods. The double-labelling technique was characterized by its samplity, no injury for cellular activity and ability for analysing the expression of the single antigen and double antigens on cell surface concurrently.
Shen
G X
GX
Research Center of Experimental Medicine, Tongji Medical University, Wuhan.
Dai
W J
WJ
Wang
X L
XL
Su
N
N
Zhu
H F
HF
Ye
W X
WX
Li
J W
JW
D000328:Adult; D000911:Antibodies, Monoclonal; D005455:Fluorescent Antibody Technique; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D012397:Rosette Formation; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens
Studie über ein Doppelmarkierungsverfahren zur Bestimmung von T-Lymphozytensubpopulationen.