[Influences of intraventricular injection of 6-hydroxydopamine on passive-avoidance response, acetylcholine, and muscarinic receptors in mouse brain].
In step-through and step-down tasks, icv 6-hydroxydopamine (6-OHDA) 20 micrograms on d 14 before training impaired the learning and memory processes in mice. But the amnesic effects in mice received icv 6-OHDA were overcome by ip scopolamine (Scop) 0.5 mg.kg-1 at 15 min before trial. When mice were pretreated with icv 6-OHDA the content of acetylcholine (ACh) in the brain showed no change, whereas the ACh-depleting action of Scop (2 mg.kg-1) was diminished. [3H]Quinuclidinyl benzilate binding assay indicated that the brain muscarinic receptor density (Bmax) and Kd in the mice after icv 6-OHDA were respectively decreased 48% and 57% as compared to the icv sterile water group. These findings suggested a possible participation of brain cholinergic nervous system in memory impairment induced by icv 6-OHDA in mice.
Pan
S Y
SY
Department of Pharmacology, Beijing College of Traditional Chinese Medicine.
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects*; D001921:Brain / Q000378:metabolism*; D006892:Hydroxydopamines / Q000494:pharmacology*; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice; D011976:Receptors, Muscarinic / Q000187:drug effects*; D012153:Retention, Psychology / Q000187:drug effects; D012601:Scopolamine / Q000494:pharmacology
D006892:Hydroxydopamines; D011976:Receptors, Muscarinic; D012601:Scopolamine; D000109:Acetylcholine