title: Role of phagocytes in antimicrobial defence of the middle ear.
abstract: The role of phagocytes in the antimicrobial defence of the middle ear was investigated in this experiment, using Hartley strain guinea pigs with an experimental otitis media. Otitis media was induced with an inoculation of Streptococcus pneumoniae into the tympanic cavity through the ear drum. For depletion of peripheral blood phagocyte population such as monocytes and polymorphonuclear neutrophils (PMNs), whole body irradiation (250 rad or 500 rad) was carried out on guinea pigs three days before S. pneumoniae inoculation into the middle ears. Carrageenan was also used for selective depletion of mononuclear cells, to distinguish their role from polymorphonuclear neutrophils. In control animals, otitis media was induced reproducibly with middle ear inoculation of more than 10(6) S. pneumoniae. In irradiated animals, which underwent 10(2) or 10(4) S. pneumoniae inoculation, the incidence of otitis media because of S. pneumoniae infection became higher in accordance with the dosage of irradiation. However, no significant difference was seen in the occurrence of otitis media and the number of viable bacteria recovered from bulla washings between controls and carrageenan-treated animals. These results suggest that phagocytes, particularly neutrophils, are essential for antimicrobial defense at the early phase of the middle ear infection with S. pneumoniae.
authors.affiliation: Department of Otolaryngology, Medical College of Oita, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kaneda
authors.forename: H
authors.initials: H
authors.lastname: Kawauchi
authors.forename: G
authors.initials: G
authors.lastname: Mogi
mesh_terms: D000818:Animals; D002351:Carrageenan / Q000494:pharmacology; D006168:Guinea Pigs; D009000:Monocytes / Q000187:drug effects; D009504:Neutrophils / Q000528:radiation effects; D010033:Otitis Media / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D010586:Phagocytes / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D013296:Streptococcus pneumoniae / Q000302:isolation & purification; D014916:Whole-Body Irradiation
chemical_list: D002351:Carrageenan