Immunoregulatory functions of paf-acether. VI. Dual effect on human B cell proliferation.
The role of paf-acether (paf), a phospholipid cytokine, in the modulation of human B cell function was investigated. Paf, from 1 x 10(-5) M to 10(-6) M, decreased B cell proliferation induced by both phorbol myristate acetate (PMA) and anti-IgM antibodies (anti-IgM Ab). By contrast, 1 x 10(-7) M to 1 x 10(-9) M paf enhanced PMA triggered, but not anti-IgM triggered B cell proliferation. B cell proliferation was modulated between 24 and 72 hr of culture indicating that the effect of paf did not merely reflect a shift in proliferation kinetics. Interestingly, paf also enhanced the spontaneous proliferation of a Burkitt lymphoma-derived B cell line, Raji, which suggests that paf can directly act on B cells. The modulatory effect of paf on peripheral blood B cells was independent of PMA concentration, yet the effect on Raji cells was dependent upon cell density. The data suggest that paf is a potent modulator of B cell function, and may be involved in the control of humoral immune response.
Leprince
C
C
Laboratoire d'Immunopathologie et d'Immunologie Virale, Universite Paris-Sud, Clamart, France.
Vivier
E
E
Treton
D
D
Galanaud
P
P
Benveniste
J
J
Richard
Y
Y
Thomas
Y
Y
D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002051:Burkitt Lymphoma; D002460:Cell Line; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects*; D010972:Platelet Activating Factor / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D010972:Platelet Activating Factor; D014316:Tritium; D013755:Tetradecanoylphorbol Acetate; D013936:Thymidine