[New possibilities for studying complex macromolecular structures of the cell by encasing cells in polyacrylamide microspheres].
A method is proposed for incorporation of mammalian cells into spheres permeable for protein molecules, the dimensions of these spheres not overcoming those of the cells. The method permits fractionation of the cells, preserving them as discrete units resistant to mechanical and hydrodynamic destruction in the course of routine laboratory manipulations, as well as using flow cytometry for their analysis. With this approach, the flow cytometric measurements were made of DNA being shielded in the chromatin complex which prevents from binding fluorescent dyes with low-molecular weights.
Filatov
M V
MV
Varfolomeev
D B
DB
Kotlovanova
L V
LV
D000180:Acrylic Resins; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D004247:DNA / Q000378:metabolism; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005782:Gels; D006367:HeLa Cells / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism / Q000648:ultrastructure*; D046911:Macromolecular Substances; D008863:Microspheres; D010539:Permeability; D051381:Rats
D000180:Acrylic Resins; D005782:Gels; D046911:Macromolecular Substances; C016680:polyacrylamide gels; D004247:DNA
Novye vozmozhnosti izucheniia makromolekuliarnykh kompleksnykh struktur kletki putem zakliucheniia kletok v poliakrilamidnye shariki.