Ecdysteroid-stimulated synthesis and secretion of an N-acetyl-D-glucosamine-rich glycopeptide in a lepidopteran cell line derived from imaginal discs.
Hormone-regulated processing of N-acetyl-D-glucosamine was studied in an insect cell line derived from imaginal wing discs of the Indian meal moth, Plodia interpunctella (Hübner). The cell line, IAL-PID2, responded to treatment with 20-hydroxyecdysone with increased incorporation of GlcNAc into glycoproteins. Cycloheximide and tunicamycin counteracted the action of the hormone. In particular, treatment with 20-hydroxyecdysone resulted in the secretion of a 5,000 dalton N-acetyl-D-glucosamine-rich glycopeptide by the IAL-PID2 cells. Accumulation of this peptide was prevented by the use of teflubenzuron, a potent chitin synthesis inhibitor. A glycopeptide of similar molecular weight was observed in imaginal discs of P. interpunctella treated with 20-hydroxyecdysone in vitro, under conditions that induce chitin synthesis. Although the function of the 5,000 dalton glycopeptide is not known, we believe that the PID2 cell line is a promising model for molecular analysis of ecdysteroid-regulated processing of aminosugars by epidermal cells during insect development.
Porcheron
P
P
Cytophysiologie des Arthropodes, Université Pierre et Marie Curie, Paris, France.
Morinière
M
M
Coudouel
N
N
Oberlander
H
H
D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D004441:Ecdysterone / Q000494:pharmacology*; D006020:Glycopeptides / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008970:Molecular Weight; D009036:Moths; D011499:Protein Processing, Post-Translational; D014316:Tritium
D006020:Glycopeptides; D014316:Tritium; D004441:Ecdysterone; D003513:Cycloheximide; D007930:Leucine; D000117:Acetylglucosamine