[Oncogene-directed mutagenesis in vivo. Polyalkylating derivatives of short single-stranded polynucleotides, complementary E1-adeno-oncogene, in the normalization of adenovirus-transformed rodent cell lines].
Polyalkylating derivatives of single-stranded polynucleotides (30-200-mers) complementary to the long E1 oncogene sequences of simian adenovirus SA7 cause inherited normalization of SH2 and G11 cells transformed with adenovirus SA7; certain deletions in the integrated proviral E1A oncogene were observed in several cases during this process. The transformed cells are indifferent to reagents noncomplementary to the E1 region. Thus polyalkylating derivatives of single-stranded 30-200-mers act as addressed mutagenes which react in a specific way with the integrated complementary DNA sequences of E1 oncogene in transformed rodent cells and realize oncogene-directed mutagenesis in vivo. During this treatment temporary normalized cells reverting to the initial transformed phenotype are also produced.
Pantin
V I
VI
Solov'ev
G Ia
GIa
Sats
N V
NV
Surin
V L
VL
Borovkova
T V
TV
Krutov
A A
AA
Zhukova
E L
EL
Grineva
N I
NI
D000261:Adenoviruses, Simian; D000477:Alkylating Agents; D000818:Animals; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004277:DNA, Single-Stranded / Q000235:genetics* / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012377:Rodentia
D000477:Alkylating Agents; D004277:DNA, Single-Stranded
Onkogen-naprovlennyĭ mutagenez in vivo. Polialkiliruiushchie proizvodnye korotkikh odnotiazhevykh polinukleotidov, komplementarnykh E1- adenoonkogenu, v normalizatsii transformirovannykh adenovirusom kloetochnykh liniĭ gryzunov.