Gas chromatography method for the separation of amino acids enantiomers in plasma and urine. Application in a case of short bowel syndrome.
Urinary amino acids were isolated from the urine of healthy controls and a patient with a short bowel syndrome. Following derivatization with isopropyl alcohol/HCl and trifluoroacetic anhydride the amino acid enantiomers were separated by gas chromatography on a Chirasil-L-Val column. All subjects excreted D-alanine (10-30% of total Ala). The percentage D-alanine was higher in the patient with the short bowel syndrome. The excretion of D-alanine did not correlate with the D-lactate excretion. An intestinal origin for the D-amino acids is the most probable explanation.
Ketting
D
D
University Children's Hospital 'Het Wilhelmina Kinderziekenhuis', Utrecht, The Netherlands.
Wadman
S K
SK
Spaapen
L J
LJ
Van der Meer
S B
SB
Duran
M
M
D000293:Adolescent; D000328:Adult; D000409:Alanine / Q000097:blood / Q000652:urine; D000596:Amino Acids / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D000621:Aminoisobutyric Acids / Q000652:urine; D002648:Child; D002675:Child, Preschool; D002849:Chromatography, Gas / Q000379:methods*; D004755:Enterobacteriaceae / Q000378:metabolism; D006801:Humans; D007223:Infant; D007422:Intestines / Q000382:microbiology; D007773:Lactates / Q000652:urine; D019344:Lactic Acid; D012016:Reference Values; D012778:Short Bowel Syndrome / Q000097:blood / Q000378:metabolism* / Q000652:urine; D013237:Stereoisomerism
D000596:Amino Acids; D000621:Aminoisobutyric Acids; D007773:Lactates; D019344:Lactic Acid; D000409:Alanine; C033435:3-aminoisobutyric acid