Modulatory effect of synthetic human IgG Fc peptides on the in vitro immune response of murine spleen cells.
Synthetic peptides representative of defined surface-exposed sequences within the CH-2 and CH-3 domains of human IgG1 induce IgM production by murine spleen cells, even in cultures depleted of T-lymphocytes. This stimulation was not altered by simultaneous administration of dextran sulphate in suboptimal concentration, its effect being additive to that of the peptides. Cell proliferation was augmented only at 10(-4) M doses of peptides. IL-1 production by adherent cells was also increased as a result of peptide treatment; whilst administration of exogenous IL-1, 4 h later, seemed to abrogate the effect of peptide treatment on the augmentation of IgM production. Peptide treatment failed to induce IL-2 and/or IL-4 production. The effect of IgG peptides seems to be exerted directly on B-cells at an early step of activation and to be mediated at least in part by IL-1.
Kádár
J
J
Second Department of Medicine, Semmelweis University, Budapest, Hungary.
Sármay
G
G
Rajnavölgyi
E
E
Stanworth
D
D
Gergely
P
P
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D005260:Female; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000008:administration & dosage* / Q000737:chemistry; D007074:Immunoglobulin G / Q000008:administration & dosage* / Q000737:chemistry; D007075:Immunoglobulin M / Q000096:biosynthesis; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007378:Interleukins / Q000096:biosynthesis / Q000494:pharmacology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology; D013154:Spleen / Q000276:immunology
D000276:Adjuvants, Immunologic; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007378:Interleukins; D010446:Peptide Fragments; D007213:Indomethacin