Inhibition of growth by the antihormone RU486 in different hepatoma cell lines.
The synthetic steroid RU486 (17 beta-hydroxy-11 beta-(4-dimethylaminophenyl)-17 alpha-(1-propynyl)-estra-4,9-dien-3-one), which has been shown to display antiprogestin and antiglucocorticoid properties in different systems, exerts antiglucocorticoid effects and inhibits the cell growth in a concentration-dependent manner on Reuber rat hepatoma cell variants. This effect can be observed on glucocorticoid-sensitive cells, containing glucocorticoid receptors, and on glucocorticoid-resistant cells displaying a very low level of dexamethasone binding. Metabolization of RU486 occurs in different glucocorticoid-resistant hepatoma variants; these cells are less sensitive to the growth inhibitory effect of the antihormone than the steroid-sensitive cells which do not metabolize RU486. Thus, metabolization of RU486 must also be taken into account for the efficacy of this antagonist on cell growth.
Chasserot-Golaz
S
S
Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France.
Beck
G
G
Venetianer
A
A
D000616:Aminoglutethimide / Q000494:pharmacology; D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D007700:Kinetics; D008114:Liver Neoplasms, Experimental; D015735:Mifepristone / Q000494:pharmacology*; D051381:Rats; D014407:Tumor Cells, Cultured
D000616:Aminoglutethimide; D015735:Mifepristone