title: Quantitation of circulating angiotensin II using commercial immunoassays.
abstract: The quantitation of circulating angiotensin (ANG) II is not consistent among various commercially available kits. We measured plasma concentrations with three radioimmunoassay kits (Bühlmann Laboratories, Basel, Switzerland; Immuno Technology Service Production, The Netherlands, and Amersham, UK). The antibody specificity and their cross-reactions with ANG I decapeptide and with fragments of ANG II, were evaluated. The antibodies of the three kits cross-reacted with nearly all immunoreactive fragments of ANG II with intact carboxyl end. Cross-reaction with ANG I was detected with the antibody of the Immuno Technology Service kit only.
authors.affiliation: Ludwig Boltzmann Institute for Clinical Endocrinology, Vienna, Austria.
authors.forename: E
authors.initials: E
authors.lastname: Templ
authors.forename: H
authors.initials: H
authors.lastname: Templ
mesh_terms: D000803:Angiotensin I / Q000097:blood; D000804:Angiotensin II / Q000097:blood*; D006801:Humans; D011863:Radioimmunoassay / Q000592:standards*; D011933:Reagent Kits, Diagnostic / Q000592:standards*
chemical_list: D011933:Reagent Kits, Diagnostic; D000804:Angiotensin II; D000803:Angiotensin I