[Effect of polymyxin B on the hemolytic mechanism of the genus Listeria].
The contact between polymyxin B and human blood cells produced a double effect on the haemolytic activity of Listeria monocytogenes, L. seeligeri and L. ivanovii hemolysins. At low polymyxin B concentrations the antibiotic promoted erythrocyte lysis whereas at higher antibiotic levels, hemolysis inhibition was observed. According to the effects of polymyxin B, hemolytic profiles were established for each hemolysin. L. monocytogenes and L. seeligeri exhibited a similar profile with maximum hemolytic activity at 1000 U/ml polymyxin B. L. ivanovii displayed a different profile, with two maximum hemolytic activities: one similar to that showed by L. monocytogenes and L. seeligeri at 1000 U/ml, and another peak of hemolysis at 4000 U/ml polymyxin B.
Thomas
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Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Santa Fe, Argentina.
D001427:Bacterial Toxins; D006360:Heat-Shock Proteins / Q000502:physiology; D006460:Hemolysin Proteins / Q000502:physiology; D006461:Hemolysis; D006863:Hydrogen-Ion Concentration; D008087:Listeria / Q000187:drug effects* / Q000502:physiology; D011112:Polymyxin B / Q000494:pharmacology*; D013045:Species Specificity
D001427:Bacterial Toxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D011112:Polymyxin B; C038705:hlyA protein, Listeria monocytogenes
Efecto de polimixina B sobre el mecanismo hemolítico del género Listeria.