Day and nighttime concentrations of 5-methoxytryptophol and melatonin in the retina and pineal gland from different classes of vertebrates.
5-Methoxytryptophol (ML) and melatonin concentrations were determined by radioimmunoassay in the retinae and pineal glands of frogs (Rana esculenta), chickens (Gallus domesticus), quails (Coturnix coturnix), and European hamsters (Cricetus cricetus), killed in the afternoon (1500-1700 hr) and around midnight (2400-0200 hr). ML was only detectable in significant amounts in the frog retina. In the pineal gland ML levels showed the opposite profile to retinal ML, with measurable levels in the chicken, quail, and hamster and undetectable levels in the frog (less than 2.0 pg/gland). A significant day/night variation was observed in the bird pineal only, with higher levels occurring during the day. In contrast to ML, the interspecies variation in melatonin content was similar in the retina and pineal, with the highest concentrations occurring in the chicken greater than quail much greater than frog greater than hamster. Significantly higher night levels of melatonin were observed in the retina of the chicken and quail and in the pineal of the chicken, quail, and frog. The ratio of ML:melatonin concentrations in the retina and pineal also showed interspecies variations. In the birds the amount of ML was minimal in comparison with the amount of melatonin (1-4% retina; 0.3-12% pineal). The highest ML:melatonin occurred in the retina of the frog and in the pineal of the European hamster.
Skene
D J
DJ
URA-CNRS 1332 Neurobiologie des fonctions rythmiques et saisonnières, Université Louis Pasteur, Strasbourg, France.
Vivien-Roels
B
B
Pevet
P
P
D000818:Animals; D002645:Chickens; D003370:Coturnix; D006224:Cricetinae; D007211:Indoles / Q000032:analysis*; D008027:Light; D008297:Male; D008550:Melatonin / Q000032:analysis*; D010507:Periodicity; D010870:Pineal Gland / Q000378:metabolism*; D011863:Radioimmunoassay; D011893:Rana esculenta; D012160:Retina / Q000378:metabolism*
D007211:Indoles; C002465:methoxytryptophol; D008550:Melatonin