title: Growth kinetics of Bu3 cells secreting HBsAg in microcarrier culture.
abstract: The genetically engineered mammalian cell (Bu3) secreting Hepatitis B surface antigen (HBsAg) was cultured on microcarriers semi-continuously. The growth model of Bu3 cells propagating on microcarriers, glucose consumption and lactate accumulation models were established. It is illustrated that the technology of anchorage-dependent Bu3 cell culture on microcarriers will give high yield and high HBsAg expression.
authors.affiliation: Research Institute of Biochemical Engineering, East China University of Chemical Technology, Shanghai.
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Shi
authors.forename: S
authors.initials: S
authors.lastname: Yuan
authors.forename: J
authors.initials: J
authors.lastname: Ding
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line / Q000378:metabolism; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods*; D004274:DNA, Recombinant; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics
chemical_list: D004274:DNA, Recombinant; D006514:Hepatitis B Surface Antigens