New method for detachment and quantitation of Acanthamoeba trophozoites in culture.
Trophozoites of two Acanthamoeba polyphaga strains (RYD and 435) were readily detached from plastic culture flasks or multiwell plates by 5 min exposure to a detaching fluid containing 0.005% of Triton X-100 and 0.05% ammonium sulphate. This treatment also led to rounding of cells and thus permitted convenient and reproducible counting by either hemocytometer or electronic particle counter. The detaching fluid did not impair viability even after exposure up to 1 h.
Ogbunude
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Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
Asiri
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Baer
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Warhurst
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D000048:Acanthamoeba / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D000645:Ammonium Sulfate / Q000494:pharmacology; D000818:Animals; D003902:Detergents / Q000494:pharmacology; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D015203:Reproducibility of Results; D013499:Surface Properties
D003902:Detergents; D011092:Polyethylene Glycols; D017830:Octoxynol; D000645:Ammonium Sulfate