title: Actin-depolymerizing factor (ADF) in the cerebellum of the developing rat: a quantitative and immunocytochemical study.
abstract: A specific antiserum against actin-depolymerizing factor (ADF) was used in a quantitative and immunocytochemical study of ADF in the cerebellum of developing rats. The Triton-soluble ADF concentration remained stable throughout development. Light and electron microscopic immunocytochemistry showed that ADF was not detected in all cerebellar cells. ADF immunoreactivity was found in Purkinje cells, but not in granule cells. It was found in the Bergmann astrocytes and the astrocytes of the white matter, but not in the oligodendrocytes. The cell bodies and dendrites of Purkinje cells were immunoreactive for ADF but the axons were not. In contrast, the other axons of the white matter (mossy and climbing fibres) were labeled. Thus, ADF was not restricted to either the dendritic or axonal compartments. However, dendritic spines and postsynaptic densities were immunoreactive, whereas presynaptic varicosities were unlabeled. The immunoreactivities for ADF and actin were compared. ADF staining was uniformly distributed throughout the entire dendritic arborization of the Purkinje cell, while filamentous actin is highly concentrated in the dendritic spines, indicating that ADF activity might vary according to its cellular localization.
authors.affiliation: Laboratoire de Neurobiologie Endocrinologique, URA 1197 CNRS, Université Montpellier II, Sciences et Techniques du Languedoc, France.
authors.forename: J Y
authors.initials: JY
authors.lastname: Léna
authors.forename: J R
authors.initials: JR
authors.lastname: Bamburg
authors.forename: A
authors.initials: A
authors.lastname: Rabié
authors.forename: C
authors.initials: C
authors.lastname: Faivre-Sarrailh
mesh_terms: D051339:Actin Depolymerizing Factors; D000199:Actins / Q000032:analysis; D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D002525:Cerebellar Cortex / Q000166:cytology / Q000254:growth & development / Q000648:ultrastructure; D002531:Cerebellum / Q000166:cytology / Q000254:growth & development* / Q000648:ultrastructure; D051345:Destrin; D007150:Immunohistochemistry; D008840:Microfilament Proteins / Q000032:analysis*; D016253:Microscopy, Immunoelectron; D009419:Nerve Tissue Proteins / Q000032:analysis; D011689:Purkinje Cells / Q000166:cytology / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D051339:Actin Depolymerizing Factors; D000199:Actins; D051345:Destrin; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins