title: Experimental infection in mice of Cryptosporidium muris isolated from a camel.
abstract: Cryptosporidium muris-like oocysts in 5 g of camel feces were concentrated by the Sheather sugar flotation method, saline-washed, pelleted and reconstituted to an approximate concentration of 2.5 million per ml. One- to 2-microliters inocula were given per os to 25 2- to 20-day-old mice, with 15 contact control litter-mates. The 15- and 20- day inoculates had acid-fast-positive fecal smears by day-12 after inoculation. Necropsies of mice inoculated at 2 and 5 days of age showed colonization only of gastric glands by day-12, and day-27, respectively. Control mice were negative. Identical methods using similar oocysts from cattle produced no evidence of transmission.
authors.affiliation: University of Idaho, Caine Veterinary Teaching and Research Center, Caldwell, Idaho 83605.
authors.forename: B C
authors.initials: BC
authors.lastname: Anderson
mesh_terms: D000818:Animals; D002162:Camelus / Q000469:parasitology*; D002417:Cattle / Q000469:parasitology; D003457:Cryptosporidiosis / Q000469:parasitology* / Q000473:pathology; D003458:Cryptosporidium / Q000302:isolation & purification*; D005753:Gastric Mucosa / Q000473:pathology; D051379:Mice