Construction and evaluation of a self-luminescent biosensor.
The genes encoding bioluminescence (lux genes), derived from the marine bacterium V. fischeri, have been fused next to the genes encoding mercury detoxification (mer genes), derived from a clinical isolate of S. marcescens. The fusion has been made so that the expression of the light genes comes under the control of the mer regulatory gene and promoter. These genetic elements activate the expression of the light genes in the presence of mercury. The light can readily be collected and quantitated, resulting in a biosensor for the detection of mercury.
Geiselhart
L
L
Department of Biology Rowley Labs, Clarkson University, Potsdam, New York 13699.
Osgood
M
M
Holmes
D S
DS
D015374:Biosensing Techniques; D003001:Cloning, Molecular; D005069:Evaluation Studies as Topic; D005798:Genes, Bacterial; D008163:Luminescent Measurements; D009876:Operon; D010957:Plasmids; D014733:Vibrio / Q000235:genetics