Specific binding and internalization of monoclonal antibody HI98-daunorubicin conjugate by human leukemic cells (HL60).
McAb-HI98 has been proved to bind specifically to HL60 cells. In this study, we further observed the specific binding of HI98-Daunorubicin (DNR) conjugate to HL60 cells, the transmembranal transport of HI98-DNR and the process of internalization by immunoelectron microscopy. After HL60 cells were incubated with HI98-DNR-Au, gold particles were first found on the surface membranes of HL60 cells, and then inside the cells. Internalization was found to occur through the formation of endocytic vesicles. Results indicate that the immunoconjugate selectively binds to target cells and enters the cells specifically and rapidly, thus enhancing intracellular drug concentration.
Liu
J
J
Institute of Hematology, CAMS, Tianjin.
Zhu
Z
Z
Liu
J
J
Yin
S
S
Chu
Y
Y
Qi
S
S
Yang
C
C
Chen
Z
Z
D000911:Antibodies, Monoclonal; D003630:Daunorubicin / Q000494:pharmacology*; D006801:Humans; D000922:Immunotoxins / Q000378:metabolism*; D007951:Leukemia, Myeloid / Q000473:pathology*; D016253:Microscopy, Immunoelectron; D014407:Tumor Cells, Cultured / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000922:Immunotoxins; D003630:Daunorubicin