In vitro release and tissue levels of ileal serotonin after cisplatin-induced emesis in the cat.
We have studied the peripheral mechanisms responsible for the severe vomiting observed in cisplatin treated cats. Release of 5-hydroxytryptamine from isolated portions of the ileum and ileal tissue concentrations of 5-hydroxytryptamine were studied in vitro with control animals and animals previously treated with cisplatin. In the latter, two groups were observed: animals in which cisplatin (single dose, 10 mg/kg) induced vomiting within 4 h and animals that did not vomit within 4 h. Spectrofluorimetric assessment of 5-hydroxytryptamine in the perfusate and ileal tissue revealed that cisplatin increases both release, and tissue levels, of ileal 5-hydroxytryptamine in animals which vomited, whereas only ileal 5-hydroxytryptamine release was increased in animals which did not vomit. This may have clinical implications as cisplatin induced emesis may be more effectively prevented by drugs preventing ileal 5-hydroxytryptamine formation rather than release.
Milano
S
S
Département de Physiologie et de Neurophysiologie, CNRS URA 205, Faculté des Sciences et Techniques Saint Jérôme, Marseille, France.
Simon
C
C
Grélot
L
L
D000818:Animals; D002415:Cats; D002945:Cisplatin; D005260:Female; D007082:Ileum / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D012701:Serotonin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014839:Vomiting / Q000139:chemically induced / Q000378:metabolism*
D012701:Serotonin; D002945:Cisplatin