Determination of urinary 2- and 3-dechloroethylated metabolites of ifosfamide by high-performance liquid chromatography.
In vivo oxidation of chloroethyl side-chains on ifosfamide produces the toxin chloroacetaldehyde. Production of this labile metabolite can be indirectly quantitated by monitoring the excretion of the residual 2- and 3-dechloroethylated ifosfamide. Urinary ifosfamide and the two dechloroethylated metabolites were extracted into chloroform from alkalinized salt-saturated urine, followed by high-performance liquid chromatographic separation using an acetonitrile gradient on a reversed-phase column and ultraviolet detection at 190 nm. In five patients given 1.6 g/m2 ifosfamide, 11-30% of the dose was excreted over 24 h as unchanged drug, 11-21% as 3-dechloroethylated and 3-10% as 2-dechloroethylated ifosfamide.
Goren
M P
MP
Department of Pathology and Laboratory Medicine, St. Jude Children's Research Hospital, Memphis, TN 38101.
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007069:Ifosfamide / Q000378:metabolism* / Q000627:therapeutic use / Q000652:urine; D009369:Neoplasms / Q000188:drug therapy / Q000652:urine; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D007069:Ifosfamide