title: Crimean-Congo haemorrhagic fever virus replication in adult Hyalomma truncatum and Amblyomma variegatum ticks.
abstract: The kinetics of the replication of the Crimean-Congo haemorrhagic fever virus (CCHFV) was studied in intra-anally inoculated adult Hyalomma truncatum and Amblyomma variegatum ticks. The virus was re-isolated by suckling mouse inoculation and revealed by antigen capture with ground ticks and indirect immunofluorescence of haemolymph. The virus was detected in ticks in the first hours post-inoculation (p.i.) and its replication was observed from 36 h p.i. onwards. Virus titre reached a maximum within 3-5 days then decreased slowly to a level of at 2 log LD50/ml for several months until the end of observations. Several specific, non-identified factors seem to favour CCHFV replication in H. truncatum. Long-term virus persistence seems to occur in CCHFV-infected adult ticks.
authors.affiliation: Institut Français de Recherche scientifique pour le Développement en Coopération (ORSTOM), Dakar.
authors.forename: J P
authors.initials: JP
authors.lastname: Gonzalez
authors.forename: J P
authors.initials: JP
authors.lastname: Cornet
authors.forename: M L
authors.initials: ML
authors.lastname: Wilson
authors.forename: J L
authors.initials: JL
authors.lastname: Camicas
mesh_terms: D000818:Animals; D001179:Arthropod Vectors / Q000382:microbiology; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000502:physiology*; D006479:Hemorrhagic Fever, Crimean / Q000382:microbiology; D007700:Kinetics; D051379:Mice; D013987:Ticks / Q000382:microbiology*; D014779:Virus Replication