Presence of platelet-activating factor in pyuria in humans.
The relationship between the occurrence of platelet-activating factor (PAF) and neutrophils in urine from patients with urinary tract infection was examined. PAF was detected in human pyuria, when leukocyte levels reached at least 300 cells/microL (n = 45), but not in normal urine (n = 12). The amount of PAF found in pyuria, measured by platelet aggregation assay, was 0.01 to 13.3 pmol/mL. A close correlation was seen between the amount of PAF present and the number of urinary leukocytes (p less than 0.01, r = 0.70). The leukocytes in pyuria consisted almost entirely of neutrophils (96 +/- 4%, mean +/- S.D.). Our findings suggest that the occurrence of PAF is associated with the accumulation of neutrophils in urine.
Ikeda
I
I
Department of Internal Medicine, Saiseikai Izuo Hospital, Osaka, Japan.
Oda
M
M
Sakakura
M
M
Yasunaga
K
K
D000818:Animals; D002855:Chromatography, Thin Layer; D006801:Humans; D007700:Kinetics; D010742:Phospholipid Ethers / Q000494:pharmacology; D010743:Phospholipids / Q000302:isolation & purification / Q000494:pharmacology / Q000652:urine*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology / Q000652:urine*; D010974:Platelet Aggregation / Q000187:drug effects; D011726:Pyridinium Compounds / Q000494:pharmacology; D011776:Pyuria / Q000652:urine*; D011817:Rabbits
D010742:Phospholipid Ethers; D010743:Phospholipids; D010972:Platelet Activating Factor; D011726:Pyridinium Compounds; C053321:CV 6209; C037913:CV 3988