title: Nonuniform size distribution of nascent globin peptides, evidence for pause localization sites, and a contranslational protein-folding model.
abstract: Examination of nascent globin peptides accumulating in vitro during globin synthesis in rabbit reticulocyte lysates was carried out. A view was supported that nonrandom distribution of codons with different usage frequencies in mRNA may determine the messenger's translation kinetics. Regions of reduced translation of alpha- and beta-globin polypeptide chains were localized, and the cotranslational protein-folding model suggested previously was substantiated. An active conjunction of synthesis and folding of proteins was proposed as one of the main destinations of a translation nonuniformity.
authors.affiliation: Department of Molecular Biology, Faculty of Biology, Moscow State University, USSR.
authors.forename: I A
authors.initials: IA
authors.lastname: Krasheninnikov
authors.forename: A A
authors.initials: AA
authors.lastname: Komar
authors.forename: I A
authors.initials: IA
authors.lastname: Adzhubei
mesh_terms: D000818:Animals; D003062:Codon; D005914:Globins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D015394:Molecular Structure; D014176:Protein Biosynthesis; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
chemical_list: D003062:Codon; D012333:RNA, Messenger; D005914:Globins