Kinetic characteristics of beta-carotene uptake after an injection of beta-carotene in pigs.
Two experiments were conducted to study the uptake of beta-carotene in plasma, lipoproteins, and blood cells in pigs (50 to 55 kg; n = 40) after an i.m. injection of 0, 10, 20, or 40 mg of beta-carotene. Blood was sampled at 0, 3, 6, 12, 24, and 48 h postinjection. beta-Carotene was not detectable in plasma, lipoproteins, or blood cells of control pigs. However, concentrations of beta-carotene in plasma and lipoproteins increased in a dose-related manner in injected animals. Distribution of beta-carotene in the lipoproteins changed with time postinjection. The beta-carotene associated with very low density lipoproteins increased and that in low density lipoproteins decreased with time in treated pigs. Concentrations of beta-carotene in lymphocytes of treated pigs also increased within 3 h postinjection. The profile of beta-carotene in lymphocytes was different from that observed in plasma and lipoproteins. Carotene was not detectable in neutrophils and erythrocytes. Treatment did not alter concentrations of retinol or alpha-tocopherol in plasma, lipoproteins, or blood cells. Therefore, lymphocytes specifically take up beta-carotene, thereby suggesting a possible role of beta-carotene in this immune cell.
Chew
B P
BP
Dept. of Anim. Sci. Washington State University, Pullman 99164-6320.
Wong
T S
TS
Michal
J J
JJ
Standaert
F E
FE
Heirman
L R
LR
D000818:Animals; D001773:Blood Cells / Q000378:metabolism*; D002338:Carotenoids / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D007273:Injections, Intramuscular / Q000662:veterinary; D008074:Lipoproteins / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D013552:Swine / Q000378:metabolism*; D014801:Vitamin A / Q000097:blood; D014810:Vitamin E / Q000097:blood; D019207:beta Carotene
D008074:Lipoproteins; D019207:beta Carotene; D014801:Vitamin A; D014810:Vitamin E; D002338:Carotenoids