Morphometric evaluation of the myocardial interstitial space after physical and chemical fixation. A light-microscopic study on the left ventricle of canine hearts.
The interstitial space, separated into tissue clefts and interstitium within bundles of myocytes of left canine ventricle, was investigated qualitatively and morphometrically after using different methods of fixation following aortic cross clamping and 10 min of ischemia at 5 degrees C. In frozen sections, the size of tissue clefts is significantly lower than in semithin sections after immersion fixation. The interstitium within bundles of myocytes shows similar values independent of the fixation technique (r = 0.83). This constancy permits a free choice between the two fixation techniques, there being no loss of information from the material investigated.
Schmiedl
A
A
Zentrum Anatomie, Abteilung Elektronenmikroskopie, Universität Göttingen, BRD.
Bach
F
F
Richter
J
J
Schnabel
P A
PA
Bretschneider
H J
HJ
D000818:Animals; D015925:Cryopreservation / Q000379:methods; D004285:Dogs; D005109:Extracellular Matrix / Q000648:ultrastructure*; D005404:Fixatives; D006352:Heart Ventricles / Q000166:cytology; D008853:Microscopy / Q000379:methods; D009206:Myocardium / Q000166:cytology*
D005404:Fixatives