[The effect of pregnant mare's serum and chorionic gonadotropic hormone on the capacity of bovine oocytes, matured in vitro, for parthenogenetic activation by cycloheximide].
The effect of chorionic gonadotropic hormone (HG) and pregnant mare serum (PMS) on the capacity of cattle oocytes for spontaneous maturation and parthenogenetic formation of the female pronucleus was studied in situ and in vitro. HG added to the culture medium had no effect, irrespective of the dosage. However, perfusion of cow ovaries with HG at a dose of 2 micrograms/ml for 1 h abolished the block of meiosis I in 80% oocytes. About 25% oocytes reached metaphase II (MII) and telophase II (TII); 30% oocytes acquired the capacity to form female pronucleus upon the action of cycloheximide (25 Mg/ml). Perfusion of cow ovaries with PMS at a dose of 5 MU/ml for 1 h resulted in elimination of the block of meiosis I in 95% oocytes; 26% oocytes reached MII and TII, and about 17% oocytes acquired the capacity to form female pronucleus.
Koval'
T Iu
TIu
Abliatipova
G N
GN
Khamidov
D Kh
DKh
D000818:Animals; D002417:Cattle; D002453:Cell Cycle / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D003513:Cycloheximide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D006238:Haploidy; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D010312:Parthenogenesis / Q000187:drug effects*
D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D003513:Cycloheximide
Vliianie syvorotki zherebykh kobyl i khoriogonicheskogo gormona na sposobnost' ootsitov korov, sozrevshikh in vitro, k partenogeneticheskoĭ aktivatsii tsiklogeksimidom.