[The effect of different factors on the reproduction of influenza viruses and reassortants in cell cultures].
The influence of the maintenance medium, polyethylene glycol (PEG), DEAE-dextran, and low temperature on reproduction of influenza A, B, and C viruses and their reassortants in diploid and continuous cell cultures was determined. Lowering of pH in the maintenance medium to 6.5 was found to decrease reproduction of influenza A (H1N1) and A (H3N2) viruses and increase that of influenza B viruses. Treatment of cells with PEG solution increased the yield of influenza B and C but not A viruses. However, influenza A virus strains proved to be capable of producing infectious progeny in nonpermissive cell lines treated with PEG. Addition of DEAE-dextran to the medium exerted no effect on the infectivity of influenza A and B reassortants. Moreover, infection of MDCK cells after a "cold shock" led to an increase in hemagglutinin titres in influenza A reassortants.
Podcherniaeva
R Ia
RIa
Danlybaeva
G A
GA
D000818:Animals; D002478:Cells, Cultured / Q000382:microbiology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003470:Culture Media; D003637:DEAE-Dextran / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009980:Influenza A virus / Q000187:drug effects / Q000502:physiology*; D009981:Influenza B virus / Q000187:drug effects / Q000502:physiology*; D009982:Gammainfluenzavirus / Q000187:drug effects / Q000502:physiology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D016865:Reassortant Viruses / Q000187:drug effects / Q000502:physiology*; D013696:Temperature; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects / Q000502:physiology*
D003470:Culture Media; D011092:Polyethylene Glycols; D003637:DEAE-Dextran
Vliianie razlichnykh faktorov na reproduktsiiu virusov grippa i reasortantov v kletochnykh kul'turakh.