Fully automated high-performance liquid chromatographic analysis of whole blood and plasma samples using on-line dialysis as sample preparation. Determination of oxytetracycline in bovine and salmon whole blood and plasma.
A fully automated technique for high-performance liquid chromatographic analysis of whole blood and plasma is described. Samples are automatically injected into a dialyser where proteins and blood cells are removed. The dialysates are concentrated on a small column prior to analysis. This technique is used for the determination of oxytetracycline in whole blood and plasma. After dialysis oxytetracycline and the internal standard, tetracycline, are retained on a polystyrene enrichment column and subsequently separated on a polystyrene analytical column by ion-pair chromatography. Using ultraviolet detection 50 ng/ml can be detected. Validation showed good within-day and between-day accuracy and precision. Different oxytetracycline concentrations were found in plasma and whole blood. This difference varied between the species.
Agasøster
T
T
Institute of Pharmacy, University of Oslo, Norway.
Rasmussen
K E
KE
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D010118:Oxytetracycline / Q000097:blood*; D006435:Renal Dialysis; D015203:Reproducibility of Results; D012474:Salmon; D013056:Spectrophotometry, Ultraviolet
D010118:Oxytetracycline