Purification of in vitro excysted Sarcocystis sporozoites by passage through a modified DE 52 anion-exchange column.
A method of purifying Sarcocystis sporozoites following in vitro excystation is described. After passage of sporozoites through a modified DE 52 anion-exchange cellulose column, the mean recovery was 88% for S. capracanis, 71% for S. hircicanis, 77% for S. ovicanis and 74% for S. arieticanis. Most of the sporozoites were motile, and the parasite suspension was free of excystation debris.
Ono
K
K
Department of Clinical Pathobiology, Faculty of Agriculture, University of Tokyo, Japan.
Horn
K
K
Heydorn
A O
AO
D000818:Animals; D002852:Chromatography, Ion Exchange; D012522:Sarcocystis / Q000302:isolation & purification*; D012523:Sarcocystosis / Q000469:parasitology*