title: Fast and simple anion-exchange chromatography for large-scale purification of self-complementary oligonucleotides.
abstract: A fast and simple anion-exchange chromatography method is described for large-scale purification of synthetic oligonucleotides. Using a single matrix and aqueous solvent system, the two-step chromatographic procedure can handle complex separation problems of self-complementary or G-rich sequences without the use of urea or formaldehyde. The work also demonstrates the complication encountered, possibly due to hairpin formation, in one of the oligomers.
authors.affiliation: Jawaharlal Nehru University, New Delhi, India.
authors.forename: A
authors.initials: A
authors.lastname: Banerjee
authors.forename: H S
authors.initials: HS
authors.lastname: Bose
authors.forename: K B
authors.initials: KB
authors.lastname: Roy
mesh_terms: D001426:Bacterial Proteins; D001483:Base Sequence; D002852:Chromatography, Ion Exchange / Q000379:methods*; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000302:isolation & purification*; D012685:Sepharose
chemical_list: D001426:Bacterial Proteins; D009838:Oligodeoxyribonucleotides; D012685:Sepharose; D015247:Deoxyribonuclease HindIII; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific