In vivo and in vitro studies on modulation of the pineal endocrine function by L-acetyl-carnitine in the rat.
Male Sprague-Dawley rats injected (i.p.) at 1500h with L-acetyl-carnitine in doses of 10, 30 or 90 mg/kg exhibited a notable increase in their pineal and serum melatonin content 1 hr later. Likewise, L-acetyl-carnitine administered in the same dose range induced a significant increase of pineal and serum melatonin content in rats treated at 0100h, following exposure of 30 min to bright white light to suppress endogenous melatonin. Under in vitro experimental conditions, however, 60 min of coincubation of isolated rat pineal glands with L-acetyl-carnitine (10(-5) M) did not result in an elevation in melatonin accumulated in the incubation medium. These results demonstrate that, in vivo, L-acetyl-carnitine can exert a modulatory action on synthesis and release of melatonin, possibly by modifying noradrenergic transmission and signal transduction in the pineal gland.
Fraschini
F
F
Department of Pharmacology, University of Milan, Italy.
Esposti
D
D
Demartini
G
G
Scaglione
F
F
Lucini
V
V
Mariani
M
M
Stankov
B
B
Mancia
M
M
D000108:Acetylcarnitine / Q000494:pharmacology*; D000818:Animals; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D008297:Male; D008550:Melatonin / Q000097:blood*; D010870:Pineal Gland / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains
D000108:Acetylcarnitine; D008550:Melatonin