title: The multicatalytic proteinase (prosome, proteasome): comparison of the eukaryotic and archaebacterial enzyme.
abstract: Proteasomes isolated and purified from rat muscle tissue and from the archaebacterium Thermoplasma acidophilum have a very similar size and shape, but the subunit composition is less complex in the archaebacterium as compared to the eukaryotic particle. The archaebacterial enzyme contains a catalytic site with chymotryptic specificity, which is inhibited by serine proteinase inhibitors and clearly differs from the eukaryotic particle which has a minimum of three catalytic sites for peptide bond hydrolysis of a yet undefined mechanism.
authors.affiliation: Diabetes Forschungsinstitut, Düsseldorf, F.R.G.
authors.forename: B
authors.initials: B
authors.lastname: Dahlmann
authors.forename: F
authors.initials: F
authors.lastname: Kopp
authors.forename: L
authors.initials: L
authors.lastname: Kuehn
authors.forename: R
authors.initials: R
authors.lastname: Hegerl
authors.forename: G
authors.initials: G
authors.lastname: Pfeifer
authors.forename: W
authors.initials: W
authors.lastname: Baumeister
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000737:chemistry / Q000378:metabolism*; D009132:Muscles / Q000201:enzymology; D009842:Oligopeptides / Q000737:chemistry; D046988:Proteasome Endopeptidase Complex; D011487:Protein Conformation; D051381:Rats; D013045:Species Specificity; D013379:Substrate Specificity; D013822:Thermoplasma / Q000201:enzymology*
chemical_list: D009097:Multienzyme Complexes; D009842:Oligopeptides; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex