Bullous pemphigoid antigen expression in Pam 212 cells induced by the addition of platelet activating factor.
Platelet activating factor (Paf-acether) is a phospholipid which has various activities including platelet and neutrophil aggregation and eosinophil chemotaxis. We have previously reported that the blister fluids of bullous pemphigoid possess platelet aggregation activity and suggested that Paf-acether might be concerned with the accumulation and activation of neutrophils and eosinophils. In this study, we examined the influence of Paf-acether on BP antigen expression on Pam 212 cells. Paf-acether enhanced the expression of BP antigen on Pam 212 cells and this expression was blocked by Paf-acether antagonist. Our observations might suggest that Paf-acether contributes to the blister formation not only by the activation of inflammatory cells but the enhancement of BP antigen.
Karashima
T
T
Department of Dermatology, University School of Medicine, Kurume, Japan.
Okubo
K
K
Hachisuka
H
H
Sasai
Y
Y
D000818:Animals; D001324:Autoantigens / Q000096:biosynthesis*; D002352:Carrier Proteins; D003094:Collagen; D003598:Cytoskeletal Proteins; D000071821:Dystonin; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000378:metabolism*; D051379:Mice; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D010391:Pemphigoid, Bullous / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000502:physiology*; D000097805:Collagen Type XVII
D001324:Autoantigens; D002352:Carrier Proteins; D003094:Collagen; D003598:Cytoskeletal Proteins; D000071821:Dystonin; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D010972:Platelet Activating Factor; D000097805:Collagen Type XVII; C095667:Dst protein, mouse