A double-label analysis demonstrating that all enkephalin-immunoreactive amacrine cells in the chicken retina express neurotensin immunoreactivity.
A previous study demonstrated less than 50% co-existence between the populations of enkephalin- and neurotensin-like immunoreactive amacrine cells in the chicken retina. The present study was undertaken with the intent of re-examining this relationship using a more sensitive double-label paradigm. An examination of retinal cryosections collected throughout the retina revealed that all labelled cells express both enkephalin and neurotensin-like immunoreactivity. Therefore, these results indicate the presence of a single population of chicken amacrine cells the members of which express both these neuropeptides.
Watt
C B
CB
Alice R. McPherson Laboratory of Retina Research, Baylor College of Medicine, The Woodlands, TX 77381.
Florack
V J
VJ
Lam
D M
DM
D000818:Animals; D000911:Antibodies, Monoclonal; D002645:Chickens; D004745:Enkephalins / Q000032:analysis*; D016650:Fluorescein-5-isothiocyanate; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry / Q000379:methods; D009496:Neurotensin / Q000032:analysis*; D012160:Retina / Q000166:cytology*
D000911:Antibodies, Monoclonal; D004745:Enkephalins; D009496:Neurotensin; D016650:Fluorescein-5-isothiocyanate