title: Intracellular recording of light responses from visually identified ganglion cells in the rabbit retina.
abstract: In this report electrophysiological recordings were made from fluorescently labeled ganglion cells in the rabbit retina. Using a retinal strip preparation, cells in the ganglion cell layer were stained following a brief application of the fluorescent dye acridine orange to the bathing solution. Through an epifluorescence microscope the tip of a recording microelectrode could be positioned near a cell of interest. Extracellular recordings from ganglion cells showed that good recovery of light responses was obtained following a brief exposure of the retina to fluorescent light (400-440 nm excitation). The rate of recovery, however, depended upon the prevailing background light level. Large acridine orange-stained cell bodies in the peripheral retina were impaled under visual control by micropipette electrodes filled with either Lucifer Yellow or the fluorescent dye pyranine. When stained intracellularly, all possessed an axon identifying them as ganglion cells. The majority (approximately 80%) of the cells recorded intracellularly were identified physiologically as either ON-center or OFF-center brisk ganglion cells. The other cells encountered were ON-OFF directionally selective ganglion cells.
authors.affiliation: Department of Biomedical Sciences, Southern College of Optometry, Memphis, TN 38104.
authors.forename: R J
authors.initials: RJ
authors.lastname: Jensen
mesh_terms: D000200:Action Potentials; D000818:Animals; D004594:Electrophysiology / Q000379:methods; D008027:Light; D008839:Microelectrodes; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D011817:Rabbits; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000502:physiology*