Short-term exposure to cadmium modifies phosphorylation of gill proteins in the sea mussel.
1. Sea mussels were exposed to cadmium for short periods of time. The excised gills were incubated with radioactive orthophosphate. The gill proteins were separated by 1- and 2-dimensional gel electrophoresis and the phosphorylation state of the proteins was determined by image analysis of autoradiographs. 2. 1-Dimensional gel electrophoresis revealed that exposure of the animals to cadmium stimulated phosphorylation of the gill proteins in a cadmium concentration-dependent manner. 3. 2-Dimensional gel electrophoresis showed that cadmium differentially affected the phosphorylation of various proteins. Major alterations were observed in the basic, high mol. wt proteins and in the acidic, low mol. wt polypeptides.
Veldhuizen-Tsoerkan
M B
MB
Department of Experimental Zoology, University of Utrecht, The Netherlands.
van der Mast
C A
CA
Holwerda
D A
DA
D000818:Animals; D049872:Bivalvia; D002104:Cadmium / Q000494:pharmacology*; D003600:Cytosol / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005880:Gills / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D011506:Proteins / Q000378:metabolism*
D011506:Proteins; D002104:Cadmium