Processed forms of neuroendocrine proteins 7B2 and secretogranin II are found in porcine pituitary extracts.
The complete structure of the novel polypeptide 7B2 recently deduced from cDNA clones has been reported to be highly conserved in a variety of species. The deduced amino acid sequence of the mature protein is predicted to be 185 or 186 amino acids long. While its biological role is still unknown, its occurrence in neuroendocrine secretory granules has been largely documented. This report shows: (i) that the protein, isolated from a large quantity of porcine pituitary glands, does not correspond to the full predicted cDNA structure but, on the contrary, to a truncated form; (ii) that the latter could arise from proteolytic cleavage at position 150 following pairs of basic residues; (iii) that it contains an extra residue at position 100 which is absent in the cDNA sequence; and, finally, (iv) that it displays a higher than expected molecular weight on SDS-polyacrylamide gel electrophoresis. In addition, a copurifying peptide was identified as an NH2-terminal related fragment of the secretogranin II molecule. Protein sequencing of the latter demonstrates (i) that the correct amino terminus of mature porcine secretogranin II is an Ala residue and not the previously proposed Gln residue and (ii) that this fragment could also arise from proteolytic cleavage at a pair of basic residues located within the secretogranin II sequence.
Lazure
C
C
J.A. De Sève Laboratory of Molecular, Neuroendocrinology, Clinical Research Institute of Montreal, Québec, Canada, University of Montreal.
Benjannet
S
S
Seidah
N G
NG
Chrétien
M
M
D000595:Amino Acid Sequence; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D002864:Chromogranins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D051859:Neuroendocrine Secretory Protein 7B2; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010902:Pituitary Gland / Q000737:chemistry*; D010907:Pituitary Hormones / Q000737:chemistry*; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry*; D013552:Swine
D014408:Biomarkers, Tumor; D002864:Chromogranins; D009419:Nerve Tissue Proteins; D051859:Neuroendocrine Secretory Protein 7B2; D010446:Peptide Fragments; D010907:Pituitary Hormones; D011506:Proteins