title: The use of polylactic acid matrix and periosteal grafts for the reconstruction of rabbit knee articular defects.
abstract: In order to find a material that would improve cartilage repair, we investigated the use of porous polylactic acid matrix (PLA) with and without periosteal grafts in large articular defects in the medial femoral condyles of 18 New Zealand white rabbit knees. The right knee defect was filled with PLA, the left defect was filled with PLA and a periosteal graft. All animals were killed at 12 weeks. PLA allowed for the de novo growth of neocartilage at the articular surface in all specimens and appeared to serve as a scaffolding for cell migration and matrix formation. Histologically, small amounts of PLA remained under the neocartilage with the majority being replaced by bone. PLA was a suitable carrier for periosteal grafts with a high graft survival rate (89%) and proliferation of a neocartilage which was thicker and more closely resembled articular cartilage than PLA alone knees. Biochemically, there was more type II collagen in the grafted knees (83%) than in the PLA alone knees (65%). Biomechanical tests of the neocartilage included equilibrium displacement, aggregate modulus, and apparent permeability. These tests were not statistically different between PLA alone and grafted knees. Comparison to normal cartilage indicated that the neocartilage was less stiff but had similar permeability. A consistent repair of the articular defects was achieved with and without periosteal grafts resulting in a tissue that closely resembled hyaline articular cartilage.
authors.affiliation: Division of Orthopaedics and Rehabilitation, University of California, San Diego.
authors.forename: H P
authors.initials: HP
authors.lastname: von Schroeder
authors.forename: M
authors.initials: M
authors.lastname: Kwan
authors.forename: D
authors.initials: D
authors.lastname: Amiel
authors.forename: R D
authors.initials: RD
authors.lastname: Coutts
mesh_terms: D000818:Animals; D016025:Bone Transplantation; D002358:Cartilage, Articular / Q000002:abnormalities* / Q000473:pathology / Q000637:transplantation; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D005270:Femur Head / Q000033:anatomy & histology; D006085:Graft Survival; D007773:Lactates; D019344:Lactic Acid; D008297:Male; D010539:Permeability; D011091:Polyesters; D011108:Polymers; D011817:Rabbits
chemical_list: D007773:Lactates; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen