Simultaneous detection of multiple single-base alleles at a polymorphic site.
PCR amplification of multiple specific alleles (PAMSA) is a rapid method of detecting single-base polymorphisms. We demonstrate its utility by detecting a polymorphism in an Alu sequence in the factor IX gene.
Dutton
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Department of Biochemistry and Molecular Biology, Mayo Clinic/Foundation, Rochester, MN 55905.
Sommer
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D000483:Alleles; D001483:Base Sequence; D004247:DNA; D005164:Factor IX / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
D005164:Factor IX; D004247:DNA