Simultaneous determination of retinol, tocopherols, carotenes and lycopene in plasma by means of high-performance liquid chromatography on reversed phase.
The determination of the vitamins A and E as well as of carotenes and lycopene is important for studies of cardiovascular diseases and cancer. A method for laboratory routine is reported to separate simultaneously retinol, tocopherols, alpha- and beta-carotene, lycopene and beta-cryptoxanthin in human plasma or serum by HPLC on reversed phase starting from one extract. Two detectors with programmable wavelength are used sequentially, a spectrophotometer for the detection of the carotenoids in the visible region and a fluorometer for the assay of retinol and the tocopherols.
Hess
D
D
Department of Vitamin and Nutrition Research, F. Hoffmann-La Roche Ltd., Basle, Switzerland.
Keller
H E
HE
Oberlin
B
B
Bonfanti
R
R
Schüep
W
W
D002338:Carotenoids / Q000031:analogs & derivatives / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D065366:Cryptoxanthins; D005470:Fluorometry; D006801:Humans; D000077276:Lycopene; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013053:Spectrophotometry; D013997:Time Factors; D014801:Vitamin A / Q000097:blood; D014810:Vitamin E / Q000097:blood; D014815:Vitamins / Q000097:blood*; D024341:Xanthophylls; D019207:beta Carotene
D065366:Cryptoxanthins; D014815:Vitamins; D024341:Xanthophylls; D019207:beta Carotene; D014801:Vitamin A; D014810:Vitamin E; D002338:Carotenoids; C041635:alpha-carotene; D000077276:Lycopene