Nucleic acids in mummified plant seeds: biochemistry and molecular genetics of pre-Columbian maize.
Nucleic acids fractions were isolated from pre-Columbian maize seeds and characterized using different approaches such as polyacrylamide gel electrophoresis, anti-DNA antibody binding, HPLC fractionation, molecular hybridization with cloned genes, and DNA amplification by the polymerase chain reaction. The nucleic acids were found to be very depolymerized (less than or equal to 140 base pairs in length) and composed mainly of ribosomal RNA. Despite the very low amount and degree of polymerization of seed DNA, specific maize nuclear Mu1, Mu4, Mu8 and, possibly, Mu5 element components could be detected, thanks to the use of amplification systems as short as 90 bp. The results suggest that evaluation of the relative proportions of Mu-type element components and, possibly, other maize genomic components in single mummified kernels, may offer a new key to the study of ancient maize populations.
Rollo
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F
Dipartimento di Biologia Molecolare, Cellulare e Animale, Università di Camerino, Italy.
Venanzi
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FM
Amici
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A
D001483:Base Sequence; D015139:Blotting, Southern; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000235:genetics*; D008969:Molecular Sequence Data; D010163:Paleontology; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics*; D012639:Seeds / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
D012335:RNA, Ribosomal; D004247:DNA