Germ line configuration of the BCL-2 major breakpoint region in hyperplastic lymph nodes.
The configuration of the BCL-2 major breakpoint region was analyzed by Southern blot hybridization and polymerase chain reaction amplification in DNA derived from 44 benign hyperplastic lymph nodes with follicular overgrowth. None of the cases exhibited translocation of the BCL-2 gene at the major breakpoint region of chromosome 18. The potential usefulness of molecular genetic detection of BCL-2 translocation in the differential diagnosis between hyperplastic lymph nodes and lymphomas is suggested.
Lubínski
J
J
Department of Genetics and Pathology, Medical Academy, Szczecin, Poland.
Ptaszynski
K
K
Tsujimoto
Y
Y
Huebner
K
K
Croce
C
C
D001483:Base Sequence; D001707:Biopsy, Needle; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002887:Chromosomes, Human, Pair 18; D006689:Hodgkin Disease / Q000473:pathology; D006801:Humans; D006965:Hyperplasia / Q000473:pathology*; D008198:Lymph Nodes / Q000473:pathology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic