Kinetochore proteins, peripheral location of chromosomes and nuclear budding: another look at the genesis of aneuploidy.
Some chromosomes in the rat do not have detectable levels of kinetochore proteins as determined by antikinetochore antibody, which may constitute a mechanism for the genesis of aneuploidy. An analysis of three mouse cell lines for the presence of kinetochore proteins showed that some chromosomes lacked these proteins, i.e. are akinetochoric by this criterion. This phenomenon may contribute to the variability of chromosome number that is characteristic of such lines. In some cells there appears to be en masse detachment of kinetochores. Several kinetochore-bearing chromosomes were also observed to be located peripherally to the spindle. These might be associated with the genesis of aneuploidy. In one cell line an apparently new phenomenon of nuclear budding was observed. In this case, one to several chromosomes appear to be pushed out of the nucleus in bud-like structures. These were surrounded by a nuclear membrane and appeared to detach from the main nucleus. Perhaps these structures eventually break off as micronuclei and, hence, would also be associated with the genesis of aneuploidy.
Vig
B K
BK
Department of Biology, University of Nevada, Reno 89557-0015.
Yoo
H J
HJ
Schiffmann
D
D
D000782:Aneuploidy; D000818:Animals; D001542:Benomyl / Q000633:toxicity; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D002875:Chromosomes / Q000378:metabolism*; D003703:Demecolcine / Q000633:toxicity; D004054:Diethylstilbestrol / Q000633:toxicity; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism*; D008941:Spindle Apparatus / Q000378:metabolism
D009687:Nuclear Proteins; D004054:Diethylstilbestrol; D001542:Benomyl; D003703:Demecolcine