One-step isolation of alpha 1-acid glycoprotein.
alpha 1-Acid glycoprotein could be isolated by a one-step extraction method from human sera and plasma. Protein recovered in the water phase after extraction with phenol at 70 degrees C for 20 min was verified as human alpha 1-acid glycoprotein when it was compared with the reference standard human alpha 1-acid glycoprotein by Ouchterlony double immunodiffusion, sodium dodecylsulfate-polyacrylamide gel electrophoresis, Western blot analysis, and periodic acid-Schiff stain. The present isolation procedure is simple and fast, and can extract about 81% of the total alpha 1-acid glycoprotein in the sera and plasma, as determined by radial immunodiffusion.
Chan
J
J
Department of Biological Sciences, California State Polytechnic University, Pomona 91768.
Yu
D
D
D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D009961:Orosomucoid / Q000302:isolation & purification*; D019800:Phenol; D010636:Phenols; D014867:Water
D009961:Orosomucoid; D010636:Phenols; D014867:Water; D019800:Phenol