Production of a monoclonal antibody using lymphocytes from Pneumocystis infected mice.
A colony of BALB/c mice maintained in a protected area of our laboratory was not infected with Pneumocystis carinii. During corticosteroid treatment, animals became infected by exposure to infected mice. After four months of corticosteroid treatment, BALB/c mice developed severe pneumocystosis. Stopping of treatment was associated with: (i) high mortality of mice, (ii) decreased lung parasite level and (iii) appearance of anti-P. carinii antibodies in survivors. A monoclonal antibody (MAb) 4F2 was obtained by immortalisation of spleen lymphocytes of a female BALB/c mouse 3 months after the cessation of corticosteroid treatment. The MAb 4F2 recognized a 210-220 kDa mouse P. carinii antigen, but did not react with rat-, rabbit- or human-derived P. carinii. This MAb reacted with all stages of mouse P. carinii.
Soulez
B
B
INSERM (U.42), Villeneuve d'Ascq, France.
Palluault
F
F
Dei-Cas
E
E
Aliouat
E M
EM
Camus
D
D
D000818:Animals; D000908:Antibodies, Fungal / Q000096:biosynthesis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D005260:Female; D007165:Immunosuppression Therapy; D008168:Lung / Q000382:microbiology; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D011010:Pneumocystis / Q000276:immunology; D011020:Pneumonia, Pneumocystis / Q000276:immunology*; D013154:Spleen / Q000166:cytology
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal