title: Adenylate cyclase responses to sucrose stimulation in membranes of pig circumvallate taste papillae.
abstract: 1. Typical adenylate cyclase (AC) responses to guanine nucleotides were found in membranes of pig circumvallate (CV) taste papillae. 2. Sucrose stimulated AC activity in the CV membranes and this stimulation was GTP dependent and tissue specific. 3. The stimulatory effect of sucrose in the CV membranes was dependent on the concentration of membranes used in the AC assay. 4. This study provides the first biochemical data on cellular transduction of taste in the pig, compares positively to preliminary results in cattle and supports recent suggestions for a role of cAMP in sweet taste transduction.
authors.affiliation: Department of Biochemistry and Human Nutrition, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
authors.forename: M
authors.initials: M
authors.lastname: Naim
authors.forename: T
authors.initials: T
authors.lastname: Ronen
authors.forename: B J
authors.initials: BJ
authors.lastname: Striem
authors.forename: M
authors.initials: M
authors.lastname: Levinson
authors.forename: U
authors.initials: U
authors.lastname: Zehavi
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D004789:Enzyme Activation; D005260:Female; D008297:Male; D013395:Sucrose / Q000502:physiology*; D013552:Swine; D013650:Taste Buds / Q000201:enzymology* / Q000648:ultrastructure
chemical_list: D013395:Sucrose; D000262:Adenylyl Cyclases