[Analysis of the activity of matrix DNA synthesis in the lymphocytes of patients after kidney transplantation and the role of the processes of degradation of DNA-dependent RNA polymerases in the overall cellular nucleolytic activity].
The activity and quantity of deoxyribonucleases in T and B lymphocyte cells isolated from peripheral blood of patients with transplanted kidney were investigated. Twenty three patients with transplanted organ, aged 35 +/- 9.7, treated with cyclosporin A and thirteen individuals after renal transplantation in age 34.8 +/- 6.3 treated conservatively were studied. The enzyme activity was defined as resting DNase activity (DNase 0). Where specifiel the reaction mixture was supplied either with 5 mM MgCl2 (DNaseMg2+) or 1 mM MnCl2 (DNase1 x Mn2+) or 2 mM MnCl2 (DNase2 x Mn2+). Two enzyme groups with molecular mass 32 kDa and 14 to 18 kDa were analyzed. It was evidenced that the nuclease activity in B lymphocyte isolated from patients treated with cyclosporin A after organ transplantation was quite close to the control subjects. On the contrary, the nucleases activity and quantity increased in T lymphocyte of the same patients and increasing in enzymes activity was depending upon haemodialysis period of time prior to organ transplantation. Enzymes activity correlate with clinical parameters typical for kidney transplant rejection. The activity and quantity of nucleases was slightly reduced in both T and B lymphocytes isolated from patients treated conservatively after organ transplantation. The useful of nuclease assay for monitoring of kidney transplant rejection is discussed.
Adamiec
R
R
Katedra i Klinika Nefrologii Akademii Medycznej we Wrocławiu.
Szewczyk
Z
Z
Szopa
J
J
D000328:Adult; D001402:B-Lymphocytes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D016572:Cyclosporine / Q000008:administration & dosage / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis* / Q000187:drug effects; D012321:DNA-Directed RNA Polymerases / Q000097:blood*; D003851:Deoxyribonucleases / Q000097:blood*; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D006084:Graft Rejection / Q000187:drug effects / Q000276:immunology; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D008875:Middle Aged; D015530:Nuclear Matrix / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D011182:Postoperative Care; D013601:T-Lymphocytes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure
D016572:Cyclosporine; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D003851:Deoxyribonucleases
Analiza aktywności enzymów syntezy matrycowego DNA w limfocytach chorych po przeszczepieniu nerek i udział procesów degradacji polimeraz RNA zaleznych od DNA w całkowitej aktywności nukleolitycznej komórki.