Effects of irradiation on marrow stromal cells with respect to committed granulocyte-macrophage progenitor cells.
The effects of irradiation on the growth regulatory function of marrow stromal cells (MSC), and on the committed granulocyte-macrophage progenitor cells (GM-CFC), were investigated using a liquid culture system. CSF activity in the supernatant of irradiated MSC (0-900 rads) increased markedly with the increase of MSC irradiation dose. CSF-inhibitory activity in the supernatant of irradiated MSC (0-900 rads) also increased with the increase of MSC irradiation dose. Furthermore, the activity of exogenous CSF added to the supernatant of 900 rad-irradiated MSC was lost more slowly than that of non-irradiated MSC. These data suggest that irradiation affected CSF production, inhibitor production and consumption of CSF by MSC.
Tsuboi
I
I
First Department of Internal Medicine, Faculty of Medicine, Nihon University.
Morimoto
K
K
Sakuma
A
A
Horie
T
T
D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects*; D001854:Bone Marrow Cells; D006098:Granulocytes / Q000528:radiation effects*; D006412:Hematopoietic Stem Cells / Q000528:radiation effects*; D066298:In Vitro Techniques; D008264:Macrophages / Q000528:radiation effects*; D008297:Male; D051379:Mice