Differential expression of migration inhibitory and migration stimulatory factors in two lines of mice genetically selected for high or low responsiveness to phytohemagglutinin. 1. Migration stimulatory factor(s) from T and B cells of immune spleen.
Expression of the lymphokine migration inhibition factor in two lines of mice genetically selected for the high (Hi/PHA) or low (Lo/PHA) response of their lymph node cells to phytohemagglutinin was found to be modulated by concomitant expression of migration stimulation factor(s) [MStF(s)]. The expression of both lymphokines was dependent on genetic character and the immunizing dose of antigen. In mice immunized 5 days earlier with 50 micrograms ovalbumin in Freund's complete adjuvant (Ova in FCA immune), migration inhibition factor, assessed with a sensitive photoelectric method, was well expressed by male spleen or lymph node 24-hour culture supernatants of Lo/PHA but Hi/PHA, especially female, expressed marked MStF(s) instead. Immunization with 500 micrograms Ova in FCA markedly enhanced expression of MStF(s) in Lo/PHA but inhibited it in Hi/PHA. MStF(s) of Ova in FCA immune spleens of the two lines were found to derive from both T and B cells, B cell activity being greater. Lo/PHA were by far better expressors of both T- and B-cell-derived MStF(s) as compared to Hi/PHA (p less than 0.01). Spleen cells of mice immunized with FCA alone also expressed MStF(s) but to lesser extent than Ova in FCA immune spleens, expression by Lo/PHA B cells being significantly higher than in Hi/PHA (p less than 0.05). The MStF(s) of Ova in FCA immune spleens was found to be non-immunoglobulin in nature.
Gauthier-Rahman
S
S
Laboratoire de Biochimie (CNRS SDI-16201), Faculté de Pharmacie, Université de Paris-Sud, Châtenay-Malabry, France.
el-Gharbi
N
N
Siddiqui
M U
MU
Couderc
J
J
Decreusefond
C
C
Stiffel
C
C
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology; D005260:Female; D005353:Fibronectins; D005455:Fluorescent Antibody Technique; D008213:Lymphocyte Activation / Q000276:immunology; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis* / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010835:Phytohemagglutinins / Q000276:immunology*; D012641:Selection, Genetic; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D016207:Cytokines; C067625:FN1 protein, human; D005353:Fibronectins; D008263:Macrophage Migration-Inhibitory Factors; D010835:Phytohemagglutinins