Up-regulation of Jun/AP-1 during differentiation of N1E-115 neuroblastoma cells.
Neuroblastoma cell lines isolated from neuroblastoma tumors can be induced to differentiate into neuronal cell types by treatment with chemical agents, such as dimethyl sulfoxide and retinoic acid. The molecular mechanisms underlying this differentiation process, however, are completely obscure. In this paper, we show that neuronal differentiation of mouse N1E-115 neuroblastoma cells by dimethyl sulfoxide is accompanied by a prolonged rise in c-jun, junB, and junD expression and AP-1 activity. Multiple sequence elements in the Jun promoters are involved in this process. Furthermore, we show that c-jun and junD, but not junB, are expressed at high levels in the neuronal cell types obtained after dimethyl sulfoxide treatment. These results suggest an important role for c-jun and junD in neuronal differentiation of N1E-115 cells.
de Groot
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RP
Laboratoire de Génétique Moléculaire des Eucaryotes, Unité 184 de l'Institut National de la Santé et de la Recherche Médicale, Faculté de Médecine, Strasbourg, France.
Kruijer
W
W
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D004247:DNA / Q000235:genetics; D004121:Dimethyl Sulfoxide; D005786:Gene Expression Regulation / Q000187:drug effects*; D016758:Genes, jun / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009447:Neuroblastoma / Q000235:genetics; D009474:Neurons / Q000187:drug effects*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D014162:Transfection / Q000235:genetics; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000235:genetics*; D001616:beta-Galactosidase
D004247:DNA; D001616:beta-Galactosidase; D004121:Dimethyl Sulfoxide