Age-dependency of infection status and serum antibody levels in human whipworm (Trichuris trichiura) infection.
This study examines the age-dependency of the relationships between human infection with whipworm (Trichuris trichiura) and parasite-specific antibody level measured by ELISA against an extract of adult worms after preincubation of the sera with Ascaris lumbricoides adult worm extract. The convex age-profile of parasite infection intensity is shown to be mirrored by an age-dependent change in age-class mean levels of IgG (all subclasses except IgG3), IgA, IgM and IgE. Mean antibody levels rise with increasing acquisition of infection in childhood and decline as the intensity of infection falls in adulthood. Immunoblot analysis of selected sera from different age-classes indicates that antigen recognition is similarly dependent on infection intensity. In individual children, antibody levels correlate positively with acquisition of infection, consistent with a simple model of antigen dosage specifying the magnitude of the humoral immune response. In adults, IgG4 correlates positively and IgA negatively with intensity of infection, suggesting involvement of these isotypes in functional roles of immune blockade or effector mechanisms, respectively.
Bundy
D A
DA
Wellcome Trust Research Centre for Parasitic Infections, Department of Biology, Imperial College, London, UK.
Lillywhite
J E
JE
Didier
J M
JM
Simmons
I
I
Bianco
A E
AE
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000097:blood; D007223:Infant; D008297:Male; D008875:Middle Aged; D010270:Parasite Egg Count; D015995:Prevalence; D014257:Trichuriasis / Q000453:epidemiology / Q000276:immunology*; D014258:Trichuris / Q000276:immunology*; D014900:West Indies / Q000453:epidemiology
D000909:Antibodies, Helminth; D007136:Immunoglobulins