title: In vitro biosynthesis of [Thr2, Leu5, D-Hiv8, Leu10]cyclosporin, a cyclosporin-related immunosuppressive peptolide.
abstract: We were able to prepare an enzyme fraction from crude extracts of the mycelium of the fungus Cylindrotrichum Bonorden, which is capable of synthesizing the new peptolide SDZ 214-103 under consumption of the constitutive amino acids, D-2-hydroxyisovaleric acid, ATP and S-adenosyl-L-methionine. The enzyme does not synthesize CyA, while cyclosporin synthetase does not synthesize the peptolide. Peptolide synthetase has a high molecular weight in the same range as cyclosporin synthetase (about 1.5 MDa).
authors.affiliation: Technical University Berlin, Institute of Biochemistry and Molecular Biology, F.R.G.
authors.forename: A
authors.initials: A
authors.lastname: Lawen
authors.forename: R
authors.initials: R
authors.lastname: Traber
authors.forename: D
authors.initials: D
authors.lastname: Geyl
mesh_terms: D000595:Amino Acid Sequence; D003524:Cyclosporins / Q000096:biosynthesis* / Q000737:chemistry; D007166:Immunosuppressive Agents / Q000737:chemistry; D003904:Mitosporic Fungi / Q000378:metabolism; D008969:Molecular Sequence Data; D015394:Molecular Structure; D008970:Molecular Weight; D010453:Peptide Synthases / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D003524:Cyclosporins; D007166:Immunosuppressive Agents; C070510:SDZ 214-103; D010453:Peptide Synthases