[The determination of protein hydrophobicity. 2. Determination of the of protein binding of sodium dodecyl sulfate with the use of the ultracentrifuge].
The interaction between sodium dodecylsulphate (SDS) and the proteins bovine serum albumin (BSA) and ovalbumin, respectively, was studied using a synthetic boundary cell of an ultracentrifuge. A method for the determination of protein-bound SDS is described. The SDS binding capacity of BSA is found to be 250 +/- 50 micrograms SDS per 500 micrograms of BSA. The amount of SDS bound by ovalbumin is so low that the procedure cannot be recommended for this protein.
Nötzold
H
H
Abteilung Chemie, Technischen Universität Dresden, Bundesrepublik Deutschland.
Kretschmar
R
R
Ludwig
E
E
D010047:Ovalbumin / Q000737:chemistry; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D014461:Ultracentrifugation
D011506:Proteins; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate; D010047:Ovalbumin
Beitrag zur Bestimmung der Hydrophobie von Proteinen 2. Mitt. Die Bestimmung von proteingebundenem Natriumdodecylsulfat mit Hilfe der Ultrazentrifuge.