Rapid high-performance liquid chromatographic method for the determination of propranolol levels in canine and feline plasma.
A sensitive high-performance liquid chromatographic method that does not require organic extraction has been developed for the determination of propranolol levels in canine and feline plasma. Equal volumes of plasma and a mixture of methanol-acetonitrile-0.1 M sodium hydroxide (3:3:4, v/v/v) were added to a microseparation unit with a 10,000 molecular mass cut-off filter. The ultrafiltrate was analyzed by reversed-phase liquid chromatography with fluorimetric detection. The consistency of the recoveries obtained eliminated the need for an internal standard (coefficients of variation less than 4%). Linear regressions for the standard curves (2.5-100 ng/ml) gave correlation coefficients above 0.9955. The detection limit was 1 ng/ml. The assay retains high sensitivity while eliminating laborious sample preparation.
Hedeen
K M
KM
Division of Pharmacology, Wellcome Research Laboratories, Research Triangle Park, NC 27709.
Tyczkowska
K
K
Aucoin
D P
DP
Norton
R M
RM
D000818:Animals; D002415:Cats; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D004285:Dogs; D008297:Male; D011433:Propranolol / Q000097:blood*; D013050:Spectrometry, Fluorescence / Q000662:veterinary
D011433:Propranolol