Determination of calcium ions tightly bound to proteins.
A rapid and sensitive procedure is described for the determination of calcium ions tightly bound to proteins using high-performance gel filtration chromatography, followed by the destabilization of the protein conformation and fluorimetric analysis with Quin-2. With this method, contaminating, unbound calcium can be eliminated simultaneously and one can determine the content of a calcium ion in a protein utilizing less than 200 pmol of the protein.
Nitta
K
K
Department of Polymer Science, Faculty of Science, Hokkaido University, Japan.
Watanabe
A
A
D000818:Animals; D002135:Calcium-Binding Proteins / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D006736:Horses; D007768:Lactalbumin / Q000032:analysis; D009113:Muramidase / Q000032:analysis; D011487:Protein Conformation
D002135:Calcium-Binding Proteins; D007768:Lactalbumin; D009113:Muramidase