N-methyl-D-aspartate stimulation of the survival of rat cerebellar granule cells in culture is not dependent upon increased c-fos expression and is not mimicked by protein kinase C activation.
The role of c-fos expression and protein kinase C in the survival of cultures of rat cerebellar granule cells was investigated. Results from immunocytochemistry and immunoblotting suggest that increased c-fos expression is not essential for the survival of cells grown in low K+ media in the presence of N-methyl-D-aspartate (NMDA) at the critical time point when sensitivity to survival requirements develops. In addition the phorbol ester, phorbol 12-myristate 13-acetate failed to bring about survival of cells cultured in low K+ media in the absence of NMDA when given chronically, suggesting that protein kinase C activation alone is not sufficient to maintain granule cell survival in culture.
Graham
M E
ME
Department of Physiology, University of Liverpool, UK.
Burgoyne
R D
RD
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects*; D003561:Cytarabine / Q000494:pharmacology; D004789:Enzyme Activation; D004790:Enzyme Induction / Q000187:drug effects; D016202:N-Methylaspartate / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D016760:Proto-Oncogene Proteins c-fos; D003561:Cytarabine; D016202:N-Methylaspartate; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D011188:Potassium