title: A comprehensive two-dimensional gel protein database of noncultured unfractionated normal human epidermal keratinocytes: towards an integrated approach to the study of cell proliferation, differentiation and skin diseases.
abstract: A two-dimensional (2-D) gel database of cellular proteins from noncultured, unfractionated normal human epidermal keratinocytes has been established. A total of 2651 [35S]methionine-labeled cellular proteins (1868 isoelectric focusing, 783 nonequilibrium pH gradient electrophoresis) were resolved and recorded using computer-aided 2-D gel electrophoresis. The protein numbers in this database differ from those reported in an earlier version due to changes in the scanning hardware (Celis et al., Electrophoresis 1990, 11, 242-254). Annotation categories reported include: "protein name" (listing 207 known proteins in alphabetical order), "basal cell markers", "differentiation markers", "proteins highly up-regulated in psoriatic skin", "microsequenced proteins" and "human autoantigens". For reference, we have also included 2-D gel (isoelectric focusing) patterns of cultured normal and psoriatic keratinocytes, melanocytes, fibroblasts, dermal microvascular endothelial cells, peripheral blood mononuclear cells and sweat duct cells. The keratinocyte 2-D gel protein database will be updated yearly in the November issue of Electrophoresis.
authors.affiliation: Institute of Medical Biochemistry, Aarhus University, Denmark.
authors.forename: J E
authors.initials: JE
authors.lastname: Celis
authors.forename: P
authors.initials: P
authors.lastname: Madsen
authors.forename: H H
authors.initials: HH
authors.lastname: Rasmussen
authors.forename: H
authors.initials: H
authors.lastname: Leffers
authors.forename: B
authors.initials: B
authors.lastname: Honoré
authors.forename: B
authors.initials: B
authors.lastname: Gesser
authors.forename: K
authors.initials: K
authors.lastname: Dejgaard
authors.forename: E
authors.initials: E
authors.lastname: Olsen
authors.forename: N
authors.initials: N
authors.lastname: Magnusson
authors.forename: J
authors.initials: J
authors.lastname: Kiil
mesh_terms: D001706:Biopsy; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional; D004730:Endothelium, Vascular / Q000473:pathology; D006801:Humans; D015151:Immunoblotting; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D011565:Psoriasis / Q000473:pathology*; D012016:Reference Values; D013545:Sweat Glands / Q000473:pathology; D015854:Up-Regulation / Q000502:physiology
chemical_list: D011506:Proteins