Cyclosporin A, effect on cytoskeleton and glycosaminoglycans in human gingival fibroblasts. Immunohistochemical and biochemical evaluation.
The effect of Cyclosporin A (CyA) in culture of human gingival fibroblasts was assessed. Extracellular and intracellular glycosaminoglycans decrease in treated cells. The ratio of non sulphated over sulphated GAGs (glycosaminoglycans) increased only in the medium. Cytoskeleton organization of treated fibroblasts was also studied by immunohistochemical means and by ultrastructural observations. Minor alterations were documented in the structure and distribution of cytoplasmic organelles. The organization of tubulin and vimentin filaments did not change in Cy A treated cells either. This study confirms the capability of Cyclosporin A to induce alterations of GAG production and synthesis with no interference in cytoplasm organization. Normal pathway GAG secretion is suggested.
Stabellini
G
G
Istituto di Istologia ed Embriologia generale, Università degli Studi, Ferrara, Italy.
Fiocchi
O
O
Evangelisti
R
R
Pagliarini
A
A
Caruso
A
A
Altavilla
G
G
D000328:Adult; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D003599:Cytoskeleton / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005881:Gingiva / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron
D006025:Glycosaminoglycans; D016572:Cyclosporine