Stereoselective high-performance liquid chromatographic assay for pirmenol enantiomers in dog plasma.
Pirmenol enantiomers in dog plasma were quantified using a stereospecific high-performance liquid chromatographic method with ultraviolet detection at 262 nm. Racemic pirmenol and internal standard, (+)-propranolol, were isolated from dog plasma by a three-step extraction procedure using toluene, 0.1 M hydrochloric acid and hexane, respectively. A chiral analytical column (Chiralcel OJ) was used with a mobile phase consisting of hexane-isopropanol-diethylamine (98.9:1.0:0.1). Linear calibration curves were obtained in the concentration range 0.0200-5.00 micrograms/ml for each enantiomer. Precision of the method, expressed as coefficient of variation for nine quality control samples, was 7.1% for (+)-pirmenol and 6.4% for (-)-pirmenol. Bias was +/- 2.2% for (+)-pirmenol and +/- 1.5% for (-)-pirmenol in quality control samples.
Janiczek
N
N
College of Pharmacy, University of Michigan, Ann Arbor 48109.
Bockbrader
H N
HN
Chang
T
T
Amidon
G L
GL
Smith
D E
DE
D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D006586:Hexanes; D006851:Hydrochloric Acid; D008297:Male; D010880:Piperidines / Q000097:blood* / Q000493:pharmacokinetics; D013237:Stereoisomerism; D014050:Toluene
D000889:Anti-Arrhythmia Agents; D006586:Hexanes; D010880:Piperidines; D014050:Toluene; D006851:Hydrochloric Acid; C024318:pirmenol