[Production of monoclonal antibodies to staphylococcal alpha-toxin using the technique of in vitro immunization].
The aim of this study is production of monoclonal antibodies (MAB) to staphylococcal alpha-toxin (SAT). SAT was obtained from culture medium of S. aureus s. 0-15 with ion-exchange chromatography and chromatofocusing. SAT was conjugated with CH-sepharose 4B and used for in vitro immunization of spleen cells, extracted from intact BALB/c mouse.
Beliaev
N N
NN
Zakir'ianova
G K
GK
Kostin
G O
GO
Tleulieva
R T
RT
Agashkin
A O
AO
Navasardiants
D G
DG
Ezepchuk
Iu V
IuV
Beklemishev
A B
AB
D000818:Animals; D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins / Q000276:immunology*; D006460:Hemolysin Proteins / Q000276:immunology*; D007114:Immunization; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009498:Neurotoxins / Q000276:immunology*; D013210:Staphylococcus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D009498:Neurotoxins; C021091:staphylococcal alpha-toxin
Poluchenie monoklonal'nykh antitel k stafilokokkovomu alfa-toksinu pri ispol'zovanii tekhniki immunizatsii in vitro.