title: Determination of cytosolic ADP and AMP concentrations and the free energy of ATP hydrolysis in human muscle and brain tissues with 31P NMR spectroscopy.
abstract: Based on experimentally determined concentrations of PCr, Pi, ATP, and H+, the ADP and AMP concentrations and the free energy for the ATP hydrolysis in normal human brain and muscle were calculated assuming that the creatine kinase- and the myokinase-catalyzed reactions were in equilibrium, accounting for changes in pH and free Mg2+ ion concentration.
authors.affiliation: Department of Medicine, University of California, San Francisco 94143.
authors.forename: K
authors.initials: K
authors.lastname: Roth
authors.forename: M W
authors.initials: MW
authors.lastname: Weiner
mesh_terms: D000244:Adenosine Diphosphate / Q000032:analysis*; D000249:Adenosine Monophosphate / Q000032:analysis*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004734:Energy Metabolism; D006801:Humans; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D009132:Muscles / Q000378:metabolism*
chemical_list: D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate