Avidin protein-conjugated column for direct injection analysis of drug enantiomers in plasma by high-performance liquid chromatography.
A new concept in high-performance liquid chromatography supports is proposed for the direct injection analysis of drug enantiomers in plasma. The new supports are designed with disuccinimidyl suberate as a hydrophobic internal region, and avidin protein as a hydrophilic and bulky surface region. Plasma proteins are excluded by the avidin phase and are eluted immediately from the column, whereas low-molecular-mass analytes can penetrate the surface region and interact with disuccinimidyl suberate. Enantiomers interact differentially with avidin, and are thereby separated. This column was used in reversed-phase high-performance liquid chromatographic analysis to determine ketoprofen enantiomers in plasma by direct injection. The recovery of racemic drug from plasma was almost 100%.
Oda
Y
Y
Department of Physical and Analytical Chemistry, Tsukuba Research Laboratories, Eisai Co., Ltd., Ibaraki, Japan.
Asakawa
N
N
Abe
S
S
Yoshida
Y
Y
Sato
T
T
D000097:Acetonitriles / Q000737:chemistry; D001360:Avidin / Q000737:chemistry*; D001798:Blood Proteins / Q000302:isolation & purification; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007660:Ketoprofen / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D000097:Acetonitriles; D001798:Blood Proteins; D002021:Buffers; D001360:Avidin; D007660:Ketoprofen; C032159:acetonitrile