Effects of PAF-antagonists in mouse ear oedema induced by several inflammatory agents.
1. Several platelet activating factor (PAF)-antagonists of different chemical structures were tested in the arachidonic acid-, tetradecanoylphorbol acetate-, dithranol-, and benzoic acid-induced mouse ear oedema models. 2. Topical application of UR-10324, UR-11353, CV-6209 and WEB-2086 markedly inhibited ear oedema induced by the four irritants tested, mimicking the profile obtained with dexamethasone. YM-461 was highly effective only in the dithranol-induced ear oedema, while BN-52021 failed to inhibit ear oedema in all models tested. 3. Leukocyte recruitment into the inflamed ears was prevented by PAF-antagonists, as measured by myeloperoxidase activity in the supernatants of ear homogenates. 4. A relationship between PAF-antagonist and anti-inflammatory activities was found in some cases, but other mechanisms cannot be excluded to explain the topical anti-inflammatory effect of these compounds. 5. Our results suggest that topical formulations containing PAF-antagonists could be useful in the treatment of some inflammatory skin diseases and provide evidence on the involvement of PAF in these inflammatory processes.
Merlos
M
M
Research Center, J. Uriach & Cía., Barcelona, Spain.
Gómez
L A
LA
Giral
M
M
Vericat
M L
ML
García-Rafanell
J
J
Forn
J
J
D000287:Administration, Topical; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D004427:Ear Diseases / Q000139:chemically induced / Q000188:drug therapy*; D004431:Ear, External; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy / Q000201:enzymology; D066298:In Vitro Techniques; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D008297:Male; D051379:Mice; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D010974:Platelet Aggregation / Q000187:drug effects; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016859:Lipoxygenase Inhibitors; D010972:Platelet Activating Factor