title: Affinity chromatography of Escherichia coli (m5U54)-methyltransferase on tRNA-agarose.
abstract: tRNA-agarose was prepared by condensing periodate-oxidized tRNA to an agarose matrix containing hydrazide functional groups. The tRNA-agarose was used to take partially purified tRNA (m5U54)-methyltransferase to homogeneity. The method is simple and reproducible and gives high yields.
authors.affiliation: Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
authors.forename: X
authors.initials: X
authors.lastname: Gu
authors.forename: D V
authors.initials: DV
authors.lastname: Santi
mesh_terms: D002482:Cellulose / Q000031:analogs & derivatives; D011232:Chemical Precipitation; D002846:Chromatography, Affinity / Q000379:methods*; D004926:Escherichia coli / Q000201:enzymology*; D011094:Polyethyleneimine; D012343:RNA, Transfer; D012685:Sepharose; D012359:tRNA Methyltransferases / Q000302:isolation & purification*
chemical_list: D011094:Polyethyleneimine; D002482:Cellulose; D012685:Sepharose; D012343:RNA, Transfer; C008881:phosphocellulose; D012359:tRNA Methyltransferases; C022769:tRNA(uracil-5)-methyltransferase