[Production of useful recombinant proteins using Namalwa KJM-1 cells adapted to serum-free medium].
We investigated the basic technology of cell culture conditions for production of useful substances such as cytokines, and related proteins produced by Namalwa cells. Namalwa cells, human B lymphoblastoid cells, were used for large scale production of alpha-interferon. Namalwa KJM-1, a subline of Namalwa cells, adapted to serum- and albumin-free medium, can grow at a high density above 1 x 10(7) cells/ml in suspension manner by the use of a perfusion culture system, Biofermenter, containing a cone-type cell-sedimentation column as cell separator. Several kinds of cytokine cDNA can be introduced and expressed in Namalwa KJM-1 cells. Some of these were produced in large quantities by use of a gene amplification method with dhfr, even through the Namalwa KJM-1 cells contained endogenous dhfr genes. For stable production of the target protein, Namalwa KJM-1 cells are very useful host cells, because they have no effective endogenous protease activity in the conditioned medium. Using Biofermenter with micro-silicone fibers and a dialysis system, the specific productivity of the target proteins was not depressed at a high cell density.
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Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
D000220:Adaptation, Biological; D000818:Animals; D002051:Burkitt Lymphoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division; D016895:Culture Media, Serum-Free; D016207:Cytokines / Q000096:biosynthesis*; D003584:Cytological Techniques; D005784:Gene Amplification; D005818:Genetic Engineering; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis; D011995:Recombination, Genetic; D014407:Tumor Cells, Cultured
D016895:Culture Media, Serum-Free; D016207:Cytokines; D011994:Recombinant Proteins