title: [Genetic system of selecting point mutations of bacteriophage T7 RNA polymerase].
abstract: Random mutagenesis of the bacteriophage T7 gene 1 was used to delineate the functionally important amino acids residues of its product--T7 RNA polymerase. A two-plasmid system has been constructed for the phenotypic selection of the mutants. Nine mutants were isolated; the RNA polymerase with Tyr-639----Asp substitution has been shown to be completely inactive. Methods were developed for the rapid purification of plasmid-encoded mutant proteins, facilitating their future biochemical analysis.
authors.forename: V O
authors.initials: VO
authors.lastname: Rechinskiĭ
authors.forename: S M
authors.initials: SM
authors.lastname: Dragan
authors.forename: D A
authors.initials: DA
authors.lastname: Kostiuk
authors.forename: D L
authors.initials: DL
authors.lastname: Liakhov
authors.forename: S N
authors.initials: SN
authors.lastname: Kochetkov
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid; D013604:T-Phages / Q000201:enzymology*
chemical_list: D012321:DNA-Directed RNA Polymerases
vernacular_title: Geneticheskaia sistema otbora tochechnykh mutantov RNK-polimerazy bakteriofaga T7.