[Studies on assessment of intensity of infection of Schistosoma japonicum in cattle].
In order to carry out quantitative epidemiological survey of animal schistosomiasis, a sensitive egg-counting method was studied both in the laboratory and in the field. Fecal material was filtered by a copper gauze (60 meshes/inch) into a nylon-tissue bag with 2 layers, 150 and 260 meshes/inch respectively. Thin smears were made from the residue in the bag. The mean recovery rate of mature schistosome eggs by previously adding 100 eggs to 5g of feces from noninfected cattle was 67.5%, ranging from 53 to 81%. To compare the egg-concentration thin smear method mentioned above, with egg-concentration thick smear method after hyalinization, various numbers of eggs, namely, 25, 50 and 100, were added to 5g of feces. The mean recovery rates of fecal eggs were 57.6%, 57.6% and 69.1% respectively, for the thin smear method and 3.2%, 13.8% and 19.5% for the thick smear method. The coefficients of variability in the former were from 12.9% to 17.4% and in the latter, from 30.7% to 98.8%. The total number of schistosome eggs per 5g of feces found in 34 egg-positive cattle in the field were 434 and 178 by the thin smear method and the thick smear method, respectively. A correlation analysis performed on the numbers of detected eggs in a total volume of fecal sediment and from half a volume of fecal sediment multiplied by two, showed a correlation coefficient of 0.98 (P less than 0.01), indicating that half a volume of fecal sediment may be used instead of a whole volume.(ABSTRACT TRUNCATED AT 250 WORDS)
Yao
B Y
BY
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine.
Qian
K
K
Zhu
H Q
HQ
Zhu
D
D
Chen
S H
SH
Zhang
R
R
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D005243:Feces / Q000469:parasitology; D010270:Parasite Egg Count / Q000379:methods / Q000662:veterinary*; D012549:Schistosoma japonicum; D012554:Schistosomiasis japonica / Q000469:parasitology / Q000662:veterinary*; D013048:Specimen Handling / Q000379:methods