Determination of 9-[(2-phosphonylmethoxy)ethyl]adenine in rat urine by high-performance liquid chromatography with fluorescence detection.
A high-performance liquid chromatographic (HPLC) method for the determination of 9-[(2-phosphonylmethoxy)ethyl]adenine (PMEA) in urine is described. The procedure includes treatment of the urine sample with chloroacetaldehyde to form the fluorescent 1,N6-ethenoadenosine derivative, which was analyzed by reversed-phase HPLC with fluorometric detection. Validation of the method showed good sensitivity, precision and reproducibility. The method is useful for the study of urinary excretion of PMEA in the rat.
Russell
J W
JW
Department of Metabolism and Pharmacokinetics, Bristol-Myers Squibb Company, Wallingford, CT 06492.
Marrero
D
D
Whiterock
V J
VJ
Klunk
L J
LJ
Starrett
J E
JE
D000225:Adenine / Q000031:analogs & derivatives* / Q000652:urine; D000818:Animals; D000998:Antiviral Agents / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D008297:Male; D063065:Organophosphonates; D051381:Rats; D011919:Rats, Inbred Strains; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D000998:Antiviral Agents; D063065:Organophosphonates; C053001:adefovir; D000225:Adenine