Functional T cell subpopulations responsible for hyposecretion of IL-2 in patients with systemic lupus erythematosus.
The ability of T cells to secrete IL-2 in patients with systemic lupus erythematosus (SLE) was investigated. In patients with SLE, impaired IL-2 production by peripheral blood lymphocytes stimulated with mitogens is well known. In this paper, we report that purified T cells stimulated with mitogens, in the presence of Epstein-Barr virus transformed B cells (B-LCL) as an accessory cell, however, could secrete a large quantity of IL-2 as much as normal T cells. In order to study this potential capacity of T cells to secrete IL-2 in patients with SLE, IL-2 secreting T cells were examined. To obtain these cells, T cells were divided into cluster forming cells and noncluster forming cells after short culture of T cells with accessory cells in the presence of Con A. Then the ability of IL-2 production in two kinds of separated T cells was examined. We found that 1) after short culture with B-LCL, the cluster forming T cells could secrete IL-2 when cultured again, but non-cluster forming T cells could not, even in the presence of B-LCL, 2) after short culture with macrophages, in normal donors and SLE patients, noncluster forming T cells were able to secrete a greater amount of IL-2 than cluster forming and undivided T cells when cultured with B-LCL.(ABSTRACT TRUNCATED AT 250 WORDS)
Hayama
T
T
Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan.
Kubo
N
N
Ikeda
E
E
Hashimoto
H
H
Sawada
S
S
Horie
T
T
D000293:Adolescent; D000328:Adult; D001773:Blood Cells / Q000378:metabolism; D002449:Cell Aggregation; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism*; D008180:Lupus Erythematosus, Systemic / Q000378:metabolism*; D016131:Lymphocyte Subsets / Q000378:metabolism*; D008875:Middle Aged; D010641:Phenotype; D013601:T-Lymphocytes / Q000378:metabolism* / Q000502:physiology
D007376:Interleukin-2