title: Polymerization and solubility of recombinant hemoglobins alpha 2 beta 2 (6Val) (Hb S) and alpha 2 beta 2(6Leu) (Hb Leu).
abstract: In an effort to clarify the role of amino acid hydrophobicity at the beta 6 position in sickling we have made recombinant hemoglobin tetramers containing beta 6 Val (Hb S) and beta 6 Leu (Hb Leu). Recombinant Hb S and Hb Leu had the same electrophoretic mobility, chromatographic behavior, and absorption spectrum. The deoxy form of both tetramers polymerized in high phosphate buffer (1.8 M) and exhibited distinct delay times prior to polymerization. The kinetics of polymerization for recombinant and native Hb S were similar, while recombinant Hb Leu polymerized more readily. The solubility of deoxy Hb Leu was less than deoxy Hb S, indicating that rapid polymerization and decreased solubility of deoxyhemoglobin is accelerated with increasing hydrophobicity at the beta 6 position.
authors.affiliation: Children's Hospital of Philadelphia, Division of Hematology, University of Pennsylvania School of Medicine, Philadelphia 19104.
authors.forename: K
authors.initials: K
authors.lastname: Adachi
authors.forename: E
authors.initials: E
authors.lastname: Rappaport
authors.forename: H S
authors.initials: HS
authors.lastname: Eck
authors.forename: P
authors.initials: P
authors.lastname: Konitzer
authors.forename: J
authors.initials: J
authors.lastname: Kim
authors.forename: S
authors.initials: S
authors.lastname: Surrey
mesh_terms: D000595:Amino Acid Sequence; D006451:Hemoglobin, Sickle / Q000737:chemistry* / Q000235:genetics; D006454:Hemoglobins / Q000737:chemistry; D006455:Hemoglobins, Abnormal / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011108:Polymers; D011993:Recombinant Fusion Proteins / Q000737:chemistry*; D012995:Solubility
chemical_list: D006451:Hemoglobin, Sickle; D006454:Hemoglobins; D006455:Hemoglobins, Abnormal; D011108:Polymers; D011993:Recombinant Fusion Proteins; C073729:hemoglobin Leu; C100003:deoxyhemoglobin