Lectin and immunolabeling of microvascular endothelia.
A number of recently developed localization techniques are beginning to be applied in the study of endothelial cells and their structural components. In this article we will review a number of these cytochemical approaches as well as their advantages and disadvantages and their applications. The methods will be presented for processing tissues for either L.R. White embedding or semi-thin and thin frozen sections followed by subsequent lectin and immunolabeling for fluorescence and electron microscopic examination. These techniques are easily applied in the localization of perfused exogenous proteins and of endogenous endothelial-associated proteins. The results that can be obtained from such studies are presented and discussed.
Milici
A J
AJ
Department of Surgery, Yale University School of Medicine, New Haven, Connecticut 06510.
Porter
G A
GA
D000818:Animals; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism*; D051379:Mice; D016253:Microscopy, Immunoelectron; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014021:Tissue Preservation
D037102:Lectins; D012710:Serum Albumin, Bovine