title: Isolation and partial characterization of antigen 5.1 from the seeds of Ricinus communis, L. (Var. Amarelo de Irece).
abstract: Antigen 5.1 was isolated from the most acidic fraction of castor bean allergens (CB-1A) by gel filtration on Sephadex G-75 followed by polyacrylamide gel electrophoresis (PAGE) (yield: 6.2 mg antigen 5.1/g CB-1A). This antigen was homogeneous by the criteria of PAGE, isoelectric focusing, SDS-PAGE, immunoelectrophoresis and gel filtration. The antigen has an apparent molecular mass of 12 +/- 2 kDa and an isoelectric point of pH 5.1. Antigen 5.1 lacks proline, phenylalanine, threonine and tryptophan. It was immunochemically identical to one of the three immunoprecipitation lines presented by CB-1A by the Ouchterlony technique, and was positive when tested (250 micrograms) by IgE-mediated passive cutaneous anaphylaxis in LOU.M rats.
authors.affiliation: Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Brasil.
authors.forename: M C
authors.initials: MC
authors.lastname: Costa-e-Silva
authors.forename: O L
authors.initials: OL
authors.lastname: Machado
authors.forename: J G
authors.initials: JG
authors.lastname: Silva-Júnior
mesh_terms: D000485:Allergens / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D000941:Antigens / Q000302:isolation & purification*; D002367:Ricinus communis / Q000276:immunology*; D002850:Chromatography, Gel; D010947:Plants, Toxic; D012639:Seeds / Q000276:immunology*
chemical_list: D000485:Allergens; D000596:Amino Acids; D000941:Antigens