title: The synthesis of chromogenic peptide substrates containing p-nitroanilides of arginine and lysine, catalyzed by proteinases adsorbed on support material.
abstract: The synthesis of the chromogenic substrates for trypsin-like proteinases catalyzed by alpha-chymotrypsin and subtilisin from B. subtilis strain 72 were carried out in the organic media at a low water content using the enzymes adsorbed on different porous materials. The method proposed allows us to vary the structure of the compounds to be synthesized and is a suitable technique for their scaling-up.
authors.affiliation: Institute for Genetics and Selection of Industrial Microorganisms, Moscow, USSR.
authors.forename: T L
authors.initials: TL
authors.lastname: Voyushina
authors.lastname: Terent'eva EYu
authors.forename: V M
authors.initials: VM
authors.lastname: Stepanov
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D000814:Aniline Compounds / Q000737:chemistry; D001120:Arginine / Q000737:chemistry; D002863:Chromogenic Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D002918:Chymotrypsin / Q000737:chemistry; D010450:Endopeptidases / Q000737:chemistry*; D004800:Enzymes, Immobilized; D008239:Lysine / Q000737:chemistry; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D013379:Substrate Specificity; D013381:Subtilisins / Q000737:chemistry
chemical_list: D000814:Aniline Compounds; D002863:Chromogenic Compounds; D004800:Enzymes, Immobilized; D009842:Oligopeptides; C019498:4-nitroaniline; D001120:Arginine; D010450:Endopeptidases; D013381:Subtilisins; D002918:Chymotrypsin; D008239:Lysine