[Immunorestitutive action of hydrolysates and ultrafiltrates of bovine spleen].
Investigations with different spleen extracts of varied composition and purity have shown immunomodulatory effects. In the present study the in vivo effect of a bovine spleen hydrolysate (Prosplen) on a radiation-induced immunodeficiency model is demonstrated. Intraperitoneal application of 1 mg/g bw hydrolysate 3/times a week caused a highly significant acceleration of the restitution of the humoral and cell-mediated immunoreactivity towards sheep erythrocytes in sublethally (600 cGy) radiated mice: plaque-forming cells in the spleen, hemagglutination, hemolysistiter of the serum, delayed type of hypersensitivity = DTH reaction. The immunoreactivity of the treated groups reached normal levels within 6-8 weeks, whereas in the groups receiving placebo this was observed after 10 weeks at the earliest. Cytofluorometric investigations revealed an accelerated restitution of the B-lymphocyte fraction in the spleen. Mice that was not irradiated showed no effect after treatment with the hydrolysate. Similar results were obtained using an ultrafiltrate of the hydrolysate.
Volk
H D
HD
Institut für Medizinische Immunologie, Medizinische Fakultät (Charité), Humboldt-Universität Berlin.
Eckert
R
R
Diamantstein
T
T
Schmitz
H
H
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D005374:Filtration; D005434:Flow Cytometry; D006386:Hemagglutination Tests; D006461:Hemolysis / Q000187:drug effects; D006462:Hemolytic Plaque Technique; D006868:Hydrolysis; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007111:Immunity, Cellular / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011833:Radiation Injuries, Experimental / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000502:physiology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D014020:Tissue Extracts / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D014020:Tissue Extracts
Immunrestitutive Wirkung eines Hydrolysates bzw. Ultrafiltrates aus Rindermilz.