title: Overexpression of L7/L12 protein with mutations in its flexible region.
abstract: Using restriction enzymes and polymerase chain reaction, three mutated forms of L7/L12 proteins with deleted 38-46, 44-52 and 38-52 residues were obtained. These mutant proteins were isolated in a preparative scale and were shown to bind to ribosomes and to affect ribosomal function.
authors.affiliation: Institute of Protein Research, Academy of Sciences of the USSR, Moscow Region.
authors.forename: A T
authors.initials: AT
authors.lastname: Gudkov
authors.forename: M G
authors.initials: MG
authors.lastname: Bubunenko
authors.forename: O I
authors.initials: OI
authors.lastname: Gryaznova
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D002872:Chromosome Deletion; D004247:DNA / Q000235:genetics; D004926:Escherichia coli; D015870:Gene Expression; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000235:genetics; D012270:Ribosomes / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D012269:Ribosomal Proteins; C016700:ribosomal protein L7-L12; D004247:DNA