[Effect of bloodletting on the proliferative activity of erythroid progenitor cells from the bone marrow of patients with polycythemia and polycythemia vera].
We assessed the effect of phlebotomy on the proliferative activity of less (BFU-E) and more (CFU-E) mature bone marrow-derived erythroid progenitors from patients with polycythemia vera (PV) and polycythemia symptomatic (PS) in vivo in diffusion chamber culture. The cloning efficiency of erythroid progenitors under the effect of normal and increased erythropoietin (Epo) concentrations in PS was comparable with controls, whereas in PV the BFU-E and CFU-E-derived colony formation was significantly higher (p less than 0.01). In PV, the erythroid progenitors formed markedly more colonies in cultures stimulated with higher Epo concentration (p less than 0.01). The results of this study indicate that phlebotomy both in PV and PS does not affect the reactivity of erythroid progenitors to various Epo concentrations.
Hansz
J
J
Kliniki Hematologii Instytutu Chorób Wewnetrznych Akademii Medycznej w Poznaniu.
Kozłowska-Skrzypczak
M
M
D000328:Adult; D000818:Animals; D001815:Bloodletting; D001853:Bone Marrow / Q000473:pathology*; D002455:Cell Division / Q000502:physiology; D003114:Colony-Forming Units Assay; D015194:Diffusion Chambers, Culture; D004906:Erythrocyte Count; D015672:Erythroid Precursor Cells / Q000473:pathology*; D005260:Female; D006410:Hematopoiesis / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010529:Peritoneal Cavity; D011086:Polycythemia / Q000097:blood* / Q000473:pathology / Q000628:therapy; D011087:Polycythemia Vera / Q000097:blood* / Q000473:pathology / Q000628:therapy; D051381:Rats
Wpływ upustów krwi na aktywność proliferacyjna prekursorów erytroidalnych szpiku chorych na czerwienice prawdziwa i objawowa.