title: The in vivo effects of culture medium. I. Radioprotective effects of vitamins, amino acids and inorganic salts of culture medium in mice.
abstract: It has been shown that pretreatment of mice with a five-fold condensed culture medium before irradiation increased the number of multipotential haemopoietic stem cells in the spleen. The degree of cyto- and radioprotection is dependent on both the time of administration and the dose of the culture medium. The administration of culture medium in twofold doses in a volume of 1 ml intraperitoneally 18 h and 8 h before irradiation with a dose of 9 Gy protected 95% of C57Bl/6 mice. On the other hand, no therapeutic effect of different doses of the culture medium was found. Our observation suggests that the culture medium can mediate a radioprotective effect. The possible mechanisms participating in this effect are discussed.
authors.affiliation: Department of Cellular and Molecular Biology, Faculty of Science, Safárik University, Kosice.
authors.forename: P
authors.initials: P
authors.lastname: Fedorocko
authors.forename: P
authors.initials: P
authors.lastname: Brezáni
authors.forename: N
authors.initials: N
authors.lastname: Macková
mesh_terms: D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D003470:Culture Media / Q000494:pharmacology*; D005260:Female; D005720:Gamma Rays; D006410:Hematopoiesis; D051379:Mice; D008810:Mice, Inbred C57BL; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D012492:Salts / Q000494:pharmacology; D014815:Vitamins / Q000494:pharmacology*; D014916:Whole-Body Irradiation
chemical_list: D000596:Amino Acids; D003470:Culture Media; D011837:Radiation-Protective Agents; D012492:Salts; D014815:Vitamins