Glycoprotein expression in mouse cerebellum: effects of inhibitors of vesicle function.
1. Explants of cerebellum from foetal mouse were cultured in vitro for 10 days in the presence of one of 5 inhibitors of vesicle function (ammonia, brefeldin A, carbonyl cyanide m-chlorophenylhydrazone, diethylcarbamazine, and monensin). 2. The effects of the inhibitors were compared with respect to: (a) the activity of enzymes involved in glycoprotein biosynthesis and degradation; (b) the expression of N-linked glycoproteins; (c) the morphology and ultrastructure of the cerebellar explants.
Carroll
M
M
Department of Biochemistry, Queen Mary and Westfield College, London, England.
Bird
M M
MM
D000641:Ammonia / Q000494:pharmacology; D000818:Animals; D020126:Brefeldin A; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002531:Cerebellum / Q000378:metabolism* / Q000648:ultrastructure; D003517:Cyclopentanes / Q000494:pharmacology; D004049:Diethylcarbamazine / Q000494:pharmacology; D004622:Embryo, Mammalian / Q000378:metabolism; D015870:Gene Expression; D006023:Glycoproteins / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D051379:Mice; D008985:Monensin / Q000494:pharmacology; D009924:Organ Culture Techniques
D003517:Cyclopentanes; D006023:Glycoproteins; D020126:Brefeldin A; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000641:Ammonia; D008985:Monensin; D006026:Glycoside Hydrolases; D004049:Diethylcarbamazine