Effects of proteinase inhibitors on polymorphonuclear neutrophil polarization.
Polarization of polymorphonuclear neutrophils (PMNs) can be elicited by the chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP) and the microtubule-disrupting compound colchicine. Here we report on whether natural and synthetic proteinase inhibitors alter the polarizing response to these two agents. The alpha 1-proteinase inhibitor, N-tosyl-L-phenylalanine chloromethyl ketone, and N alpha-tosyl-L-lysine chloromethyl ketone suppress fMLP-induced polarization and locomotion in a dose-dependent fashion, but none of them affects colchicine-induced polarization. We suggest that proteinase inhibitors suppress fMLP-induced polarization by blocking cell surface proteinases that generate an intracellular signal for cytoskeletal change and polarization.
Aoshiba
K
K
First Department of Medicine, Tokyo Women's Medical College, Japan.
Nagai
A
A
Takizawa
T
T
D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D011480:Protease Inhibitors / Q000494:pharmacology*; D014107:Tosyllysine Chloromethyl Ketone / Q000494:pharmacology; D014108:Tosylphenylalanyl Chloromethyl Ketone / Q000494:pharmacology; D000515:alpha 1-Antitrypsin / Q000494:pharmacology
D011480:Protease Inhibitors; D000515:alpha 1-Antitrypsin; D014107:Tosyllysine Chloromethyl Ketone; D014108:Tosylphenylalanyl Chloromethyl Ketone; D009240:N-Formylmethionine Leucyl-Phenylalanine; D003078:Colchicine