Determination of the amino acid sequence of cystine-containing peptides by tandem mass spectrometry.
A method has been developed for the determination of the amino-acid sequence of a cyclic peptide containing cystine. It is based on the reduction of the peptide in a reductive matrix prior to ionization by fast-atom bombardment. The amino-acid sequence of the resulting linear peptide is then determined by tandem mass spectrometry from the spectrum produced by the collision-induced decomposition of the [M + H]+ ion of the peptide.
Despeyroux
D
D
Department of Chemistry, University of Warwick, Coventry, UK.
Bordas-Nagy
J
J
Jennings
K R
KR
D000595:Amino Acid Sequence; D001127:Arginine Vasopressin / Q000032:analysis*; D008236:Lypressin / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010121:Oxytocin / Q000031:analogs & derivatives* / Q000032:analysis*
D001127:Arginine Vasopressin; C100032:tocinoic acid; D010121:Oxytocin; D008236:Lypressin