Opposite effects of bacterial lipopolysaccharide on Fc-receptor-mediated phagocytosis of two bone marrow-derived macrophage cell lines, BDM-1 and BDM-1W3.
We have reported the isolation and characterization of three factor-dependent macrophage cell lines from bone marrow cells of C3H/HeN mice. We have since isolated a subclone, BDM-1W3, from one of these cell lines. We found previously that BDM-1W3 has a different sensitivity to bacterial lipopolysaccharide (LPS) for growth than its parental cell line, BDM-1. In this report, we show that LPS inhibits BDM-1W3 phagocytosis of antibody-coated sheep erythrocytes (Fc-mediated phagocytosis), whereas it enhanced Fc-mediated phagocytosis by BDM-1. It was observed that a loss of Fc-receptor capacity parallels a loss of phagocytic activity in LPS-treated BDM-1W3 cells. LPS stimulated phagocytosis of latex beads by BDM-1 and BDM-1W3, suggesting that Fc-mediated phagocytosis and phagocytosis of latex beads differ in their regulatory mechanisms. When BDM-1 cells were cultured with LPS, they underwent drastic morphological changes, whereas LPS-treated BDM-1W3 cells did not change significantly. Gamma interferon enhanced FC-mediated phagocytosis by BDM-1, while it has no significant effect on that by BDM-1W3. These cell lines should be useful for studying signal transduction mechanisms in LPS-mediated macrophage activation.
Ohki
K
K
Department of Microbiology, Saga Medical School, Japan.
Soejima
T
T
Kohashi
O
O
Nagayama
A
A
D000954:Antigens, Surface / Q000032:analysis; D001854:Bone Marrow Cells; D002460:Cell Line; D007371:Interferon-gamma / Q000494:pharmacology*; D007840:Latex; D008070:Lipopolysaccharides / Q000633:toxicity*; D008264:Macrophages / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects*; D011961:Receptors, Fc / Q000502:physiology*; D012397:Rosette Formation; D012485:Salmonella typhi
D000954:Antigens, Surface; D007840:Latex; D008070:Lipopolysaccharides; D011961:Receptors, Fc; D007371:Interferon-gamma