title: Variants of human seminal acrosin inhibitor (HUSI-II) which inhibit human leukocyte elastase.
abstract: A cDNA coding for human seminal inhibitor II was fused to the ompA-gene leader peptide for the expression in E. coli. The secreted protein is biologically active and correctly processed. Inhibitors of human leukocyte elastase were constructed by site-directed mutagenesis.
authors.affiliation: GBF-Gesellschaft für Biotechnologische Forschung mbH, Braunschweig.
authors.forename: J
authors.initials: J
authors.lastname: Collins
authors.forename: W
authors.initials: W
authors.lastname: Taube
authors.forename: E
authors.initials: E
authors.lastname: Fink
authors.forename: A
authors.initials: A
authors.lastname: Möritz
authors.forename: H
authors.initials: H
authors.lastname: Fritz
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D066298:In Vitro Techniques; D019272:Leukocyte Elastase; D008297:Male; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors*; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000494:pharmacology; D012661:Semen / Q000378:metabolism; D015842:Serine Proteinase Inhibitors / Q000235:genetics* / Q000494:pharmacology
chemical_list: D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D011993:Recombinant Fusion Proteins; D015842:Serine Proteinase Inhibitors; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase