title: Lack of evidence for a saturable tetracycline transport system in Staphylococcus aureus.
abstract: Previous studies on tetracycline transport into Staphylococcus aureus identified a high-affinity, saturable uptake system for the antibiotic (Km, 4.76 microM) (B.L. Hutchings, Biochim. Biophys. Acta 174:734-738, 1969). However, the earlier results could not be confirmed using conditions that permitted energy-dependent, concentrative uptake of tetracycline. Kinetic artifacts introduced by inappropriate washing procedures may explain the previous results.
authors.affiliation: Department of Microbial Biochemistry and Genetics, Lederle Laboratories, American Cyanamid, Pearl River, New York 10965.
authors.forename: I
authors.initials: I
authors.lastname: Chopra
authors.forename: S
authors.initials: S
authors.lastname: Ismail
authors.forename: B
authors.initials: B
authors.lastname: Oliva
mesh_terms: D001693:Biological Transport, Active; D002478:Cells, Cultured; D004926:Escherichia coli; D013211:Staphylococcus aureus / Q000378:metabolism*; D013752:Tetracycline / Q000378:metabolism*
chemical_list: D013752:Tetracycline