Fluorometric labeling of tetrahydroprogesterones.
7-Diethylaminocourmarin-3-carbohydrazide was used to label the ketone group of the tetrahydroprogesterones to form fluorescent derivatives with high sensitivity. The four isomeric 3-hydroxypregnanes separated readily on high-performance, thin-layer chromatography after derivatization. This separation was not possible with the underivatized isomers. Standards and steroids from biologic mixtures were separated and showed similar characteristics. The methods used are described.
Touchstone
J C
JC
Department of Obstetrics and Gynecology, School of Medicine, University of Pennsylvania, Philadelphia 19104.
Fang
X
X
D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000737:chemistry*; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry*; D005470:Fluorometry; D006801:Humans; D006834:Hydrazines / Q000737:chemistry*; D011374:Progesterone / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry
D003374:Coumarins; D005456:Fluorescent Dyes; D006834:Hydrazines; C075233:7-diethylaminocoumarin-3-carbohydrazide; D011374:Progesterone