Induction of cytochrome P450 isozymes in human amnion FL cells and its application to the biological detection of mutagens.
Using AHH, EROD, ECOD and APND as marker enzymes and 3-MC, beta-NF, NE and PB as inducers, inducible cytP450 IA and IIB gene expression was demonstrated in the human amnion FL cell line; these cells possess a broad spectrum of drug-metabolizing enzymes. Maximum induction was observed following co-treatment with 3-MC and NE. Both constitutive and induced AHH were proved to have the characteristics of cytP448-dependent mixed-function oxygenases. Induced cytP450 isozyme activity remained at a high level for 24-36 h after removal of the inducer. The induced FL cells were demonstrated to activate common promutagens/procarcinogens in UDS and ADPRT-mediated decrease of NAD content assay systems. This new design can be used as a simplified mutagen screening system: no supplemental liver microsomal activation system is needed.
Yu
Y
Y
Zhejiang Branch, CAMS, Hangzhou.
Zhang
L
L
Sun
L
L
Dai
Y
Y
Fang
M
M
Chen
X
X
D000650:Amnion / Q000166:cytology / Q000201:enzymology*; D015197:Carcinogenicity Tests; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004790:Enzyme Induction; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis*; D009152:Mutagenicity Tests
D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System