Semi-continuous microcarrier culture of rCHO cells secreting HBsAg by feeding microcarriers.
On the basis of culturing rCHO cells semi-continuously with a constant concentration of microcarriers, cell yield and HBsAg expression were increased by feeding microcarriers, step by step, to provide a surface for cells to grow. A process of culturing rCHO cells semi-continuously to produce HBsAg by feeding microcarriers was established, in which the maximum microcarrier concentration was defined. The foundation has been laid for culturing rCHO cells continuously to reach high cell yield and high HBsAg expression.
Chen
Z H
ZH
Biochemical Engineering Institute, East China University of Chemical Technology, Shanghai.
Shi
Y A
YA
Ding
J C
JC
D000818:Animals; D016466:CHO Cells / Q000166:cytology / Q000378:metabolism; D002452:Cell Count; D002455:Cell Division; D003001:Cloning, Molecular / Q000379:methods*; D006224:Cricetinae; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D006511:Hepatitis B Antigens / Q000096:biosynthesis*; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism
D003470:Culture Media; D006511:Hepatitis B Antigens; D005947:Glucose; D010100:Oxygen