title: Class II MHC antigen expression by cultured human cerebral vascular endothelial cells.
abstract: Cerebral vascular endothelial cells (EC) isolated from human brain do not constitutively express class II MHC antigens. However, incubation in the presence of human interferon-gamma (IFN gamma) resulted in the expression of both HLA-DR and -DP antigens. FACS analysis revealed that approximately 40% of the EC population expressed HLA-DR antigen. Quantitation by ELISA demonstrated that maximum expression was observed with 100 U/ml IFN gamma for 4 days. Treatment with IFN gamma also increased class II mRNA levels in all EC cultures tested.
authors.affiliation: Stroke Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
authors.forename: R M
authors.initials: RM
authors.lastname: McCarron
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: E P
authors.initials: EP
authors.lastname: Cowan
authors.forename: M
authors.initials: M
authors.lastname: Spatz
mesh_terms: D015152:Blotting, Northern; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation; D004730:Endothelium, Vascular / Q000187:drug effects / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004827:Epilepsy / Q000276:immunology / Q000601:surgery; D005434:Flow Cytometry; D005802:Genes, MHC Class II / Q000187:drug effects; D006681:HLA-D Antigens / Q000032:analysis*; D006682:HLA-DP Antigens / Q000032:analysis; D006683:HLA-DQ Antigens / Q000032:analysis; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008833:Microcirculation; D011994:Recombinant Proteins; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D006681:HLA-D Antigens; D006682:HLA-DP Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D011994:Recombinant Proteins; D007371:Interferon-gamma