Platelet-activating factor acetylhydrolase activity in human tissues and blood cells.
Human tissues, blood cells, and plasma have enzymes that catalyze the hydrolysis of PAF (1-O-alkyl-2-acetyl-sn-glycero-3- phosphocholine). The activities are not due to phospholipases A2 that hydrolyze long chain acyl groups at the sn-2 position of glycerophospholipids, since they are calcium-independent and are specific for hydrolysis of short chain acyl groups. We examined the biochemical properties of these PAF acetylhydrolase activities (EC 3.1.1.47) in homogenates of human liver and spleen, in white blood cells (neutrophils and monocytes), and in erythrocytes. The data suggest that the plasma and intracellular PAF acetylhydrolase activities are likely due to different proteins. Second, the intracellular PAF acetylhydrolase activities in liver and spleen share several biochemical features that differentiate them from the activities in blood cells. Third, the activities in monocytes and neutrophils have properties that differentiate them from the activity present in human erythrocytes. Finally, the erythrocyte activity has unique properties that place it in a separate category of short chain acylhydrolases. In conclusion, there is a family of distinct enzymes that can be identified as PAF acetylhydrolases based on their calcium-independence and specificity for a short residue at the sn-2 position of phospholipids.
Stafforini
D M
DM
Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah School of Medicine, Salt Lake City 84112.
Prescott
S M
SM
Zimmerman
G A
GA
McIntyre
T M
TM
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D002122:Calcium Chloride / Q000494:pharmacology; D002850:Chromatography, Gel; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D009504:Neutrophils / Q000201:enzymology*; D010713:Phosphatidylcholines / Q000494:pharmacology; D010741:Phospholipases A / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D013154:Spleen / Q000201:enzymology*
D010713:Phosphatidylcholines; D004533:Egtazic Acid; D004492:Edetic Acid; D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D002122:Calcium Chloride