[Replication of ColE 1-related plasmids at increased growth temperature depending on rom function].
The content of ColE 1-related plasmids increased about 4-6-fold after a temperature-shift from 30 to 42 degrees C (45 degrees C) if the rom-region of the plasmids was deleted. The copy number of rom(+)-plasmids did not change after the temperature shift. All rom(-)-plasmids tested in this study showed this plasmid amplification. The Rom-protein is capable of inhibiting plasmid replication by stabilization the initial reversible stage of the association of RNA I with the primer precursor RNA II. We suggest that the temperature-dependent enhancement of the copy number of rom(-)-plasmids is due to a destabilization of this initial phase of the RNA I-preprimer interaction at high temperatures which is suppressed by the Rom-protein in cells with rom(+)-plasmids.
Riethdorf
S
S
Ernst-Moritz-Arndt-Universität Greifswald, Institut für Allgemeine und Spezielle Mikrobiologie, Bundesrepublik Deutschland.
Ulrich
A
A
Hecker
M
M
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003086:Bacteriocin Plasmids; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D012322:RNA Precursors / Q000378:metabolism; D013696:Temperature
D001426:Bacterial Proteins; D012322:RNA Precursors; C042833:ROM protein, Bacteria
Replikation ColE 1-verwandter Plasmide bei erhöhter Wachstumstemperatur in Abhängigkeit von der Rom-Funktion.