Simultaneous determination of two antioxidants, uric and ascorbic acid, in animal tissue by high-performance liquid chromatography.
A rapid and simple method for the simultaneous analysis of uric and ascorbic acid in extracts of animal tissue is described. The method uses reversed-phase ion-pair chromatography with ultraviolet detection. The technique allows efficient separation of both acids while showing high selectivity, recovery, reproducibility, and sample stability. Calculated levels of both substances in mouse liver tissue were 1.00 +/- 0.05 mumol ascorbic acid/g and 130 +/- 5 nmol uric acid/g.
Barja de Quiroga
G
G
Department of Animal Biology-II (Animal Physiology), Faculty of Biology, Complutense University, Madrid, Spain.
López-Torres
M
M
Pérez-Campo
R
R
Rojas
C
C
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D008099:Liver / Q000737:chemistry*; D008297:Male; D051379:Mice; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014527:Uric Acid / Q000032:analysis*
D000975:Antioxidants; D014527:Uric Acid; D001205:Ascorbic Acid