Protective activity of Bordetella adenylate cyclase-hemolysin against bacterial colonization.
Bordetella pertussis synthesizes several factors. It has been suggested that one of these factors, the adenylate cyclase-hemolysin (AC-Hly), directly penetrates target cells and impairs their normal functions by elevating intracellular cAMP. In the present study, we show that active immunization with purified B. pertussis AC-Hly or AC (a fragment of the AC-Hly molecule carrying only the adenylate cyclase activity but no toxin activity in vitro) protects mice against B. pertussis intranasal infection. Immunization with AC-Hly or AC significantly shortens the period of bacterial colonization of the mouse respiratory tract. Furthermore, B. parapertussis AC-Hly or AC are also protective antigens against B. parapertussis colonization; their protective activities are equivalent to that of the whole-cell vaccine. These results suggest that AC-Hly may play an important role in Bordetella pathogenesis, in a murine model. If this factor plays a similar role in the human disease, its use as a protective antigen could reduce not only the incidence of the disease, but also the asymptomatic human reservoir by limiting bacterial carriage.
Guiso
N
N
Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, Paris, France.
Szatanik
M
M
Rocancourt
M
M
D000262:Adenylyl Cyclases / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification; D001884:Bordetella / Q000201:enzymology / Q000276:immunology* / Q000472:pathogenicity; D001885:Bordetella Infections / Q000276:immunology; D001886:Bordetella pertussis / Q000201:enzymology / Q000276:immunology* / Q000472:pathogenicity; D004195:Disease Models, Animal; D005260:Female; D006460:Hemolysin Proteins / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C
D000942:Antigens, Bacterial; D006460:Hemolysin Proteins; D000262:Adenylyl Cyclases