Gastric juice and analysis of basal and stimulated secretion following treatment with rice-bran oil.
In determining whether rice-bran oil (RBO) has antisecretory activity, we induced stress ulcers in 20 Wistar rats (10 controls; 10 animals given 0.2 ml/day RBO orally for 4 days prior to ulcer induction). In turn, we analyzed gastric juice for histamine, pepsin, H+ concentration ([H+]) and output volume. A second, complementary study was made of basal and stimulated gastric secretion through continuous "in vivo" recording of output. Secretion was stimulated with increasing doses of histamine, betanechol and pentagastrin. Ninety-four rats were used (47 controls and 47 rats given 0.2 ml/day RBO orally for 4 days prior to gastric output evaluation). The results were evaluated by the Student t-test. Ulcer index in the RBO-treated rats was significantly lower than among the controls (p less than 0.01), as also reflected by a significantly greater decrease in [H+] among the RBO-treated rats (p less than 0.05). However, no significant differences were observed for the remaining parameters. Continuous recording of basal gastric output showed a significant decrease in [H+] among the RBO-treated rats (p less than 0.01). Following histamine stimulation, [H+] was likewise significantly lower among the RBO-treated rats than in the controls. However, no significant differences were observed following stimulation with either betanechol or pentagastrin. RBO contains a high percentage of unsaturated fats; the latter act as precursors in the synthesis of arachidonic acid, which in turn is the essential precursor of prostaglandins--established inhibitors of gastric secretion. RBO also contains antioxidants such as alpha-tocopherol, which may likewise stimulate the synthesis of prostaglandins. RBO likely acts by increasing prostaglandin output, thus interfering with gastric HCl production.
Lloris
J M
JM
Research Center, Valencia General and University Hospital, Spain.
Bolant
B
B
Gimeno
L
L
Cejalvo
D
D
Gimeno
L O
LO
Calvo
M A
MA
Perkins
I
I
D000284:Administration, Oral; D000818:Animals; D001626:Bethanechol Compounds / Q000494:pharmacology; D005744:Gastric Acid / Q000378:metabolism; D005750:Gastric Juice / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology; D012275:Oryza; D010418:Pentagastrin / Q000494:pharmacology; D010434:Pepsin A / Q000032:analysis* / Q000378:metabolism; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet; D013276:Stomach Ulcer / Q000378:metabolism*
D001626:Bethanechol Compounds; D010938:Plant Oils; D006632:Histamine; D010434:Pepsin A; D010418:Pentagastrin