General and selective isolation procedure for high-performance liquid chromatographic determination of anabolic steroids in tissues.
A multi-residue method has been developed for the determination of anabolic steroids in animal tissue. The analytes are extracted from tissue with methanol and the extract is subjected to two solid-phase extractions, one using a non-specific adsorbing material, such as graphitized carbon black (Carbopack B), and the other Amberlite CG-400 I in the OH form. This procedure allowed the neutral anabolics (testosterone, trenbolone and progesterone) to be isolated and separated from the acidic type (phenolic group), such as diethylstilbestrol, oestradiol, zeranol/zearalenone and their respective metabolites. The determination was effected using high-performance liquid chromatography with different detectors (ultraviolet, fluorimetric and electrochemical). Several analytical parameters were studied: chromatographic conditions, recoveries, evaporation step, solvent flow-rate, cartridges reusability, interference of plastic cartridges. For all the anabolics investigated the recoveries were greater than 83.6%.
Laganà
A
A
Department of Industrial Chemistry, University of Bologna, Italy.
Marino
A
A
D000327:Adsorption; D045930:Anabolic Agents / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D008099:Liver / Q000737:chemistry*; D008832:Microchemistry; D009132:Muscles / Q000737:chemistry*; D011786:Quality Control
D045930:Anabolic Agents