Synthesis, characterisation and high-performance liquid chromatography of C6-C16 dicarboxylyl-mono-coenzyme A and -mono-carnitine esters.
The synthesis and purification of the mono-coenzyme A and mono-carnitine esters of the homologous series of straight-chain even-numbered dicarboxylic acids (C6-C16) is described. The corresponding 3-hydroxyacyl- and 2-enoyl-CoA esters were prepared enzymatically. A reversed-phase high-performance liquid chromatographic (HPLC) system for the analysis of the intact CoA esters is described and their chromatographic behaviour documented. Reversed-phase HPLC systems for the analysis of the 4-bromophenacyl derivatives of the dicarboxylyl-mono-carnitines and the 4-nitrobenzyl derivatives of the free acids are also described. Some preliminary studies of the metabolism of [U-14C]hexadecanedionoyl-mono-CoA by rat liver peroxisomes and rat skeletal muscle mitochondria are described illustrating the application of these methods.
Pourfarzam
M
M
Department of Child Health, Medical School, University of Newcastle upon Tyne, UK.
Bartlett
K
K
D000214:Acyl Coenzyme A / Q000138:chemical synthesis* / Q000737:chemistry; D000818:Animals; D002331:Carnitine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003998:Dicarboxylic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D004952:Esters / Q000138:chemical synthesis / Q000737:chemistry; D008099:Liver / Q000737:chemistry / Q000201:enzymology; D008830:Microbodies / Q000737:chemistry / Q000201:enzymology; D008931:Mitochondria, Muscle / Q000737:chemistry / Q000201:enzymology; D051381:Rats; D016339:Spectrometry, Mass, Fast Atom Bombardment
D000214:Acyl Coenzyme A; D003998:Dicarboxylic Acids; D004952:Esters; D002331:Carnitine