Collisional spectroscopy approaches in the characterization of 7-alpha- and 7-beta-hydroxycholesterol.
High- and low-energy collision experiments, by means of double-focusing and ion-trap mass spectrometers, respectively, performed on the M+ and [M-H2O]+. ions of 7-alpha- and 7-beta-hydroxycholesterol lead to a clear structural characterization of the two stereoisomers. In particular, the beta isomer shows peculiar fragmentation pathways due to interaction between the hydroxyl group in position 7-beta and the hydrogen atom on C(15).
Favretto
D
D
CNR, Padova, Italy.
Guidugli
F
F
Seraglia
R
R
Traldi
P
P
Ursini
F
F
Sevanian
A
A
D006888:Hydroxycholesterols; D013058:Mass Spectrometry / Q000379:methods*; D013237:Stereoisomerism
D006888:Hydroxycholesterols