stf1: a new suppressor of the mitotic control gene, cdc25, in Schizosaccharomyces pombe.
A novel element in the mitotic control, stf1, has been identified genetically by its ability to rescue cdc25-22 as well as a gene disruption of cdc25. This is the first phenotypically non-wee mutation shown to do so. stf1-1 functions additively with cdc2-1w, cdc2-3w, or wee1-6 to rescue cdc25. The available data are consistent with the wild-type gene product operating either on the same pathway as cdc25 or to stimulate cdc2 by a pathway independent of cdc25 or wee1. The stf1 gene has been cloned and sequenced and encodes a putative protein of 50-65 kD, depending on whether a potential intron is present. It is a novel protein with no homology detected in the current data bases. When challenged with hydroxyurea, stf1-1 acts additively with cdc2-3w in rescuing cdc25 mutants and in allowing mitosis to occur without DNA synthesis. It does not appear to play a role in the nutritional sensing pathway nor in the pathway mediating radiation-induced G2 delay.
Hudson
J D
JD
Department of Biology, Queen's University, Kingston, Ontario, Canada.
Feilotter
H
H
Lingner
C
C
Rowley
R
R
Young
P G
PG
D000483:Alleles; D018797:Cell Cycle Proteins; D004271:DNA, Fungal / Q000096:biosynthesis / Q000187:drug effects; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006918:Hydroxyurea / Q000494:pharmacology; D008938:Mitosis / Q000235:genetics* / Q000528:radiation effects; D009154:Mutation; D009687:Nuclear Proteins; D011494:Protein Kinases / Q000235:genetics; D011505:Protein-Tyrosine Kinases; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D013489:Suppression, Genetic; D020666:ras-GRF1
D018797:Cell Cycle Proteins; D004271:DNA, Fungal; D005656:Fungal Proteins; D009687:Nuclear Proteins; D029702:Schizosaccharomyces pombe Proteins; D020666:ras-GRF1; D011494:Protein Kinases; C068689:wee1 protein, S pombe; D011505:Protein-Tyrosine Kinases; D006918:Hydroxyurea