Transepithelial water movement in response to carbamazepine, chlorpropamide and demeclocycline in toad urinary bladder.
1. Osmotic water movement across toad isolated hemibladders was measured by a gravimetric method. 2. The influence of carbamazepine, chlorpropamide and demeclocycline on the antidiuretic hormone (ADH)-induced water flow rate was examined. 3. No antidiuretic activity due to carbamazepine alone was observed but a slight inhibition due to ADH-induced water flow was observed in the presence of carbamazepine over a selected dose-range. This was unexpected and is inconsistent with data from in vivo studies in man. 4. Chlorpropamide potentiated ADH-induced water flow, in keeping with the hypothesis that chlorpropamide sensitizes the renal tubules to ADH-induced water flow. 5. Demeclocycline inhibited ADH-induced water flow. The mechanism of action remains unclear.
Hirji
M R
MR
Department of Medicine, School of Postgraduate Medicine and Biological Sciences, University of Keele, Hartshill, Stoke on Trent.
Mucklow
J C
JC
D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology; D001834:Body Water / Q000378:metabolism*; D002024:Bufo marinus; D002220:Carbamazepine / Q000494:pharmacology*; D002747:Chlorpropamide / Q000494:pharmacology*; D003707:Demeclocycline / Q000494:pharmacology*; D004357:Drug Synergism; D004848:Epithelium / Q000378:metabolism; D066298:In Vitro Techniques; D009995:Osmosis / Q000187:drug effects; D001743:Urinary Bladder / Q000378:metabolism*
D001127:Arginine Vasopressin; D002220:Carbamazepine; D003707:Demeclocycline; D002747:Chlorpropamide