Melanin-related biochemistry of IGR 1 human melanoma cells.
Cultured melanoma cells have been of great value in the study of pigment metabolism. IGR 1 human melanoma cells, established by Dr Christian Aubert, produce melanin in large quantities. These cells have been used for isolation of human tyrosinase which enzyme has not previously been obtained in a pure form. IGR 1 cells contain large amounts of 5-S-cysteinyldopa which is the quantitatively most important catecholic amino acid. This review deals with the metabolism of dopa, cysteinyldopa, glutathionyldopa, cysteine and glutathione, compounds of central importance in pigment metabolism. The information available on tyrosinase, catecholic compounds and on thiols in IGR 1 melanoma cells makes these cells most suitable for further investigation of the metabolism of human melanoma cells.
Karg
E
E
Department of Dermatology, University Medical School, Pécs, Hungary.
Odh
G
G
Rosengren
E
E
Wittbjer
A
A
Rorsman
H
H
D015102:3,4-Dihydroxyphenylacetic Acid / Q000494:pharmacology; D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D002396:Catechols / Q000378:metabolism; D003548:Cysteinyldopa / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D008543:Melanins / Q000378:metabolism*; D008545:Melanoma / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D014442:Monophenol Monooxygenase / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism; D009368:Neoplasm Transplantation; D013438:Sulfhydryl Compounds / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism*
D002396:Catechols; D008543:Melanins; D009363:Neoplasm Proteins; D013438:Sulfhydryl Compounds; D015102:3,4-Dihydroxyphenylacetic Acid; D003548:Cysteinyldopa; D014442:Monophenol Monooxygenase; D005978:Glutathione