Structure of the glycocalyx polysaccharide of Pseudomonas fragi ATCC 4973.
The structure of the exocellular glycocalyx polysaccharide of Pseudomonas fragi ATCC 4973, a bacterium implicated in the spoilage of meat, has been determined using hydrolysis, methylation analysis and 1D- and 2D-n.m.r. spectroscopy. The polysaccharide, which aids in the adhesion of the cells to each other and to the meat tissue, has the regular repeating unit ----4)-3-O-[(R)-1-carboxyethyl]-alpha-D-Glcp-(1----3)-beta-D-ManpNAc+ ++- (1----4)-beta-D-Glcp-(1----. Random partial O-acetylation occurred in some preparations of the polysaccharide.
Parolis
L A
LA
School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa.
Parolis
H
H
Dutton
G G
GG
Wing
P L
PL
Skura
B J
BJ
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006023:Glycoproteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011134:Polysaccharides / Q000737:chemistry*; D011549:Pseudomonas / Q000737:chemistry*
D006023:Glycoproteins; D011134:Polysaccharides