[The effect of detergents on the physicochemical and immunochemical properties of the isolated M1 protein of the influenza virus].
The degree of solubility of influenza virus protein M1 preparations isolated from virions by acid chloroform-methanol extraction was studied under the effect of a wide spectrum of detergents of different origin. The same detergents were used for solution of a lipid comprising a part of artificially formed liposomes. Only some of the detergents used (sodium dodecyl sulfate, SDS, triton X-100, and disintegron-B) were shown to be optimal for solution of both influenza virus protein M1 and lipid. The degree of effect on the immunochemical properties of protein ML isolated from influenza virus virion of the above-mentioned detergents optimal for solution was also studied. For this purpose, a panel of 18 monoclonal antibodies with different determinant specificity to protein M1 was used. Two of the three detergents (SDS and disintegron-B) were shown not to change the antigenic profile of protein M1. The immunochemical properties of protein M1 of influenza virus isolated from virions by two methods: chloroform-methanol extraction and preparative polyacryl amide gel electrophoresis, were studied. These two methods of protein M1 isolation were shown not to alter its immunochemical properties.
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D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis / Q000187:drug effects / Q000276:immunology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003902:Detergents / Q000494:pharmacology*; D007120:Immunochemistry; D009980:Influenza A virus / Q000737:chemistry / Q000187:drug effects* / Q000276:immunology / Q000302:isolation & purification; D008081:Liposomes; D012995:Solubility; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000187:drug effects* / Q000276:immunology; D014771:Virion / Q000737:chemistry / Q000187:drug effects / Q000276:immunology / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D003902:Detergents; D008081:Liposomes; D014763:Viral Matrix Proteins
Vliianie detergentov na fiziko-khimicheskie i immunokhimicheskie svoĭstva izolirovannogo belka M1 virusa grippa.