title: Factors affecting laboratory acclimatization of field collected Lymnaea (Bullastra) cumingiana Pfeiffer (Pulmonata: Lymnaeidae).
abstract: Lymnaea (Bullastra) cumingiana, the newly discovered natural second intermediate host of Echinostoma malayanum in the Philippines, is a sensitive and delicate lymnaeid species which requires certain conditions for successful transport from the field and cultivation in the laboratory. Field collected specimens were found to be best transported in styrofoam containers lined with wet filter paper or containing natural substrate and vegetation instead of Sphagnum moss. The method is convenient and produces a survival rate of 73-86%. However, transport time is crucial and mortality increases the longer the snails are in transit. For optimal results in laboratory acclimatization, snails are best raised in wide-mouthed containers providing a large exposed water surface area. Adequate aeration is advised but vigorous bubbling of the water should be avoided. Water should be replaced with filtered dechlorinated water every 2 to 3 days, depending on water quality. A combination of fresh lettuce leaves and a few flakes of fish food was found to be ideal. Lastly, population density was the most significant factor affecting survival and so overcrowding should be avoided.
authors.affiliation: Department of Parasitology, College of Public Health, University of the Philippines, Manila.
authors.forename: R B
authors.initials: RB
authors.lastname: Monzon
authors.forename: V
authors.initials: V
authors.lastname: Kitikoon
mesh_terms: D000064:Acclimatization; D000818:Animals; D007753:Laboratories; D008195:Lymnaea; D011156:Population Density; D012908:Snails; D013048:Specimen Handling / Q000379:methods*; D015996:Survival Rate; D013997:Time Factors