title: Purification of serum albumin with dye-ligand adsorption chromatography.
abstract: Dye molecules were coupled to agarose gel beads to form blue, red and yellow chromatography media for the separation of serum albumin. The adsorption properties of five dye-ligands were compared and the effects of temperature, pH, protein concentration and salt concentration were investigated. Human serum albumin (HSA) was purified from human placental plasma by a single step of dye-ligand chromatography under suitable operating conditions. The product was electrophoretically pure, and the elution recovery was as high as 95%. This new technique is superior to the conventional salting-out process.
authors.affiliation: Dalian University of Technology.
authors.forename: Z G
authors.initials: ZG
authors.lastname: Su
authors.forename: W
authors.initials: W
authors.lastname: Jiang
authors.forename: P S
authors.initials: PS
authors.lastname: Feng
authors.forename: K Y
authors.initials: KY
authors.lastname: Gao
mesh_terms: D000327:Adsorption; D002847:Chromatography, Agarose / Q000379:methods*; D004396:Coloring Agents; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D015394:Molecular Structure; D012709:Serum Albumin / Q000302:isolation & purification*; D013696:Temperature
chemical_list: D004396:Coloring Agents; D008024:Ligands; D012709:Serum Albumin