Effect of dimethoate on hepatic cytochrome P-450 and glutathione S-transferase activity in pigeon and rat.
Effect of acute exposure (24 hr) to different oral doses of dimethoate on hepatic microsomal cytochrome P-450 (Cyt. P-450) content and cytosolic glutathione S-transferase (GST) activity were determined in pigeon and rat to ascertain difference in the metabolic response as a measure of species selective toxicity. Dimethoate at five different doses caused a statistically significant decrease in Cyt. P-450 content both in pigeon and rat. However, reduction in GST activity was significant at three doses in pigeon and at high dose in rat. Thus, a different quantum of hepatic Cyt. P-450 decrease and a differed response of GST activity against dimethoate exposure in pigeon and rat may be one of the possible causes for relatively higher toxicity of dimethoate in birds.
Siddiqui
M K
MK
Toxicology Unit, Indian Institute of Chemical Technology, Hyderabad.
Anjum
F
F
Mehboob
M
M
Mustafa
M
M
D000818:Animals; D010856:Columbidae; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004117:Dimethoate / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase; D004117:Dimethoate