title: Direct derivative spectrophotometric determination of nitrazepam and clonazepam in biological fluids.
abstract: The use of fifth order derivative spectra allows the direct determination of nitrazepam in urine at 388 nm with a limit of detection of 1 microgram ml-1. The determination of nitrazepam in blood plasma can be carried out directly by measurement at 402 nm in the fourth order derivative spectra with a limit of detection of 1.5 micrograms ml-1. Clonazepam can be determined directly in urine samples at 384 nm by using sixth order derivative spectra with a limit of detection of 1 microgram ml-1 and in blood plasma by using fourth order derivative spectra at 384 nm with a limit of detection of 0.5 ppm.
authors.affiliation: Departamento de Química Analítica, University of Valencia, Burjassot, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Randez-Gil
authors.forename: J A
authors.initials: JA
authors.lastname: Daros
authors.forename: A
authors.initials: A
authors.lastname: Salvador
authors.forename: M
authors.initials: M
authors.lastname: de la Guardia
mesh_terms: D002998:Clonazepam / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D009567:Nitrazepam / Q000032:analysis* / Q000097:blood / Q000652:urine; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002998:Clonazepam; D009567:Nitrazepam