Histamine measurement in whole blood and cell supernatants. A comparative study of two different radioimmunoassays.
Histamine as an important mediator of the allergic reaction has been measured by a number of methods both in whole blood and cell supernatants after in vitro release. We compared the fluorometric histamine assay with two radioimmunoassays and studied detection limit, recovery, cross-reactivity and accuracy in buffer-based standards as well as the sensitivity after IgE-mediated in vitro release from washed cells and whole blood. Our data indicate that the double-antibody RIA (Pharmacia) can be used for studies of basophil histamine release in both whole blood and cell supernatants. Due to significant cross-reactivity to N-methyl-histamine it cannot be used for histamine determinations in plasma. The second radioimmunoassay using succinyl-glycine as an acylating reagent and monoclonal antibodies, which have been raised to acylated histamine, is the most sensitive assay without any cross-reactivity with methylhistamine.
Rotz
B
B
University Children's Hospital, Berlin, Germany.
Savaser
A
A
Werthmann
I
I
Lau
S
S
Wahn
U
U
D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D003429:Cross Reactions; D005470:Fluorometry; D006632:Histamine / Q000097:blood*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D006632:Histamine