Isolation of microfilariae from blood by gravitational field-flow fractionation.
Over 100 million persons suffer from diseases caused by filariae infestation, and one billion are at risk. A simple isolation method for both analytical and preparative separation is presented. Based on the simplest field-flow fractionation technique, the gravitational one, effective isolation of microfilariae is achieved. Microfilariae are eluted in the void volume of the channel without pollution by red blood cells. The red blood cell elution peak shows a total absence of microfilariae, as demonstrated after fraction collection and microscopic investigation. The elution mode of microfilariae and red blood cells appears to be a steric one, as confirmed by a reinjection experiment. The simplicity, low cost and the relatively short time required for this separation (10 min) indicate that gravitational field-flow fractionation could become a new separation tool for screening of microfilariae. With both live and dead microfilariae, the high recovery (66-80%) allows preparative fractionation for diagnostic purposes or fundamental research.
Merino
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A
Laboratoire de Chimie Analytique et d'Electrochimie Organiques, Université de Paris-Sud, Châtenay-Malabry, France.
Bories
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C
Gantier
J C
JC
Cardot
P J
PJ
D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D004605:Elephantiasis, Filarial / Q000097:blood / Q000175:diagnosis / Q000469:parasitology; D008368:Mansonelliasis / Q000097:blood / Q000175:diagnosis / Q000469:parasitology; D008842:Microfilariae / Q000302:isolation & purification*; D012377:Rodentia