Attenuation of anaphylactic shock and related mortality in guinea-pigs after administration of a potent protein kinase inhibitor, K252a.
We examined whether protein kinases have a role in the expression of anaphylactic shock (AS). Guinea-pigs sensitized to ovalbumin were administered i.p. saline (control) or 10 micrograms/kg K252a, a potent protein kinase inhibitor, 30 min before challenge. The development of AS and mortality was observed for the next 2 h. In the K252a-treated group the incidence of AS fell to 53% from 100%, the maximum intensity was 62% less than the control, and mortality dropped to 16% from 50% of the animals. We suggest that protein kinases are involved in the expression of AS, and that inhibitors of these enzymes may protect against the symptoms of AS and allergy.
Paz
O
O
Department of Hormone Research, Weizmann Institute of Science, Rehovot, Israel.
Ashkenazy
Y
Y
Moshonov
S
S
Fischer
G
G
Feigel
D
D
Kusniec
F
F
Geltner
D
D
Zor
U
U
D000707:Anaphylaxis / Q000201:enzymology* / Q000517:prevention & control; D000818:Animals; D002227:Carbazoles / Q000494:pharmacology*; D006168:Guinea Pigs; D026121:Indole Alkaloids; D008297:Male; D010766:Phosphorylation; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*
D002227:Carbazoles; D026121:Indole Alkaloids; C049985:staurosporine aglycone; D011493:Protein Kinase C