Effect of vitamin E-deficiency on the activity of some lysosomal and non-lysosomal proteases in rabbit muscles.
The activity of different cathepsins and neutral proteinases was measured in normal and vitamin E-deficient rabbit muscles using specific substrates. Among the changes of enzyme activities in dystrophy caused by vitamin E-deficiency the increase in the activity of cathepsin B is the most striking. The activity of cathepsin H, both in the fast and slow muscles and that of MMP-ase in the slow muscle remains practically unchanged. Activities of other proteases significantly increase. The change in the activity of proteolytic enzymes in striated muscle of vitamin E-deficient rabbits seems to be selective. As a rule the increase in the activity is higher in fast than in slow muscles.
Katona
G
G
2nd Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary.
Székessy-Hermann
V
V
Guba
F
F
Sohár
I
I
D000818:Animals; D002401:Cathepsin B / Q000378:metabolism*; D056655:Cathepsin H; D002403:Cathepsins / Q000378:metabolism; D003546:Cysteine Endopeptidases; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism*; D009132:Muscles / Q000201:enzymology*; D009137:Muscular Dystrophy, Animal / Q000201:enzymology / Q000209:etiology; D011817:Rabbits; D013223:Statistics as Topic; D014811:Vitamin E Deficiency / Q000201:enzymology*
D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; D056655:Cathepsin H