Purification and characterization of a neurotoxic phospholipase A2 from Indian cobra (Naja naja naja) venom.
Snake venoms contain multimolecular forms of phospholipase A2 which are diverse with respect to their pharmacological properties. A neurotoxic PLA2 from Naja naja naja venom has been purified in two steps. (1) The whole venom was fractionated on CM-Sephadex C-25 column; 4.6% of the total PLA2 activity recovered was found in the NN-V fraction. (2) The NN-Vb-PLA2 fraction was purified to homogeneity by gel filtration of fraction NN-V on Sephadex G-50. It is a basic protein with a mol. wt between 10,500 and 11,000, and is more toxic than other basic PLA2s purified from Naja naja naja venom. The LD50 of NN-Vb-PLA2 is 0.27 mg/kg body wt. It induced neurotoxic symptoms in experimental mice and is devoid of myotoxic, anticoagulant and edema-inducing activities.
Bhat
M K
MK
Department of Studies in Biochemistry, University of Mysore, India.
Prasad
B N
BN
Gowda
T V
TV
D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003039:Cobra Neurotoxin Proteins / Q000302:isolation & purification* / Q000633:toxicity; D004546:Elapid Venoms / Q000201:enzymology*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007928:Lethal Dose 50; D051379:Mice; D008970:Molecular Weight; D010741:Phospholipases A / Q000302:isolation & purification* / Q000633:toxicity; D054467:Phospholipases A2; D013050:Spectrometry, Fluorescence
D003039:Cobra Neurotoxin Proteins; D004546:Elapid Venoms; D010741:Phospholipases A; D054467:Phospholipases A2