Detection and assay of some human proteins by immunoconcentration technique.
For detection and semi-quantitative assays of some human proteins by immunoconcentration techniques, specific antibodies covalently bound to nylon microparticles and then captured in the form of small dots on glass microfibre disc, were used. The discs were placed on a water absorbant material in a simple plastic device. For the technique, antibodies labeled with peroxidase were also applied. The use of several chromogenic substrates for peroxidase was checked in the technique.
Rapak
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Biochemical Laboratory, Polish Academy of Sciences, Wrocław.
Szewczuk
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D006063:Chorionic Gonadotropin / Q000276:immunology / Q000302:isolation & purification; D002863:Chromogenic Compounds; D006735:Horseradish Peroxidase; D006801:Humans; D007120:Immunochemistry / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification; D009757:Nylons; D011506:Proteins / Q000276:immunology / Q000302:isolation & purification*; D012709:Serum Albumin / Q000276:immunology / Q000302:isolation & purification
D006063:Chorionic Gonadotropin; D002863:Chromogenic Compounds; D007074:Immunoglobulin G; D009757:Nylons; D011506:Proteins; D012709:Serum Albumin; D006735:Horseradish Peroxidase