Antigen-induced contraction of human isolated lung preparations passively sensitized with monoclonal IgE: effects of indomethacin.
Human isolated lung preparations were passively sensitized using mouse monoclonal dinitrophenyl (DNP)-specific IgE antibodies. The contractile response to antigen (DNP-bovine serum albumin; DNP-BSA) was approximately 80% of the histamine response (50 microM: 0.20 +/- 0.03 g/mm2) in bronchial muscle preparations. In passively sensitized pulmonary vascular and parenchymal preparations, the contraction to antigen was negligible. Indomethacin (1.7 microM; 30 min) did not alter the contractile response to DNP-BSA (5 micrograms/ml) in bronchial tissues. In passively sensitized bronchial muscle preparations stimulated with DNP-BSA, there was a significant increase (2-fold) in prostanoid production. This production was inhibited by indomethacin. These data suggest that endogenous prostaglandins may not play a role in the regulation of human isolated bronchial muscle contraction to antigen in vitro.
Norel
X
X
INSERM U200, Université Paris Sud, Clamart, France.
Haye-Legrand
I
I
Labat
C
C
Benveniste
J
J
Brink
C
C
D000941:Antigens / Q000502:physiology*; D001980:Bronchi / Q000502:physiology; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000008:administration & dosage; D008168:Lung / Q000502:physiology*; D009119:Muscle Contraction / Q000276:immunology; D009130:Muscle, Smooth / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D011453:Prostaglandins / Q000502:physiology
D000941:Antigens; D011453:Prostaglandins; D007073:Immunoglobulin E; D006632:Histamine