Determination of 2'-deoxy-5-iodouridine and its metabolite 5-iodouracil by high-performance liquid chromatography with ultraviolet absorbance detection in human serum.
A new assay is described for 2'-deoxy-5-iodouridine, a drug employed as an antiviral agent by topical application. The parent drug, its systemic metabolite 5-iodouracil and an internal standard (5-iodouridine) were extracted from salted serum by an ethyl acetate partition at pH 6.7, back-extracted in alkalinized water and injected into a reversed-phase column. Potassium phosphate buffer-acetonitrile (95:5, v/v) eluted the analytes at a flow-rate of 1.5 ml/min. Detection was at 290 nm. The method proved to be linear in the 100-2000 ng/ml range.
Belotto
N
N
Analytical Research Division of Farma Resa srl, Cantù, Como, Italy.
Reiner
V
V
Verga
F
F
Canobbio
G
G
Lietti
F
F
Magnani
P
P
Lucarelli
C
C
D000998:Antiviral Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007065:Idoxuridine / Q000097:blood*; D012016:Reference Values; D013056:Spectrophotometry, Ultraviolet; D014498:Uracil / Q000031:analogs & derivatives* / Q000097:blood
D000998:Antiviral Agents; D014498:Uracil; C048747:5-iodouracil; D007065:Idoxuridine