Genetic mapping of the t-complex region on mouse chromosome 17 including the Hybrid sterility-1 gene.
t-haplotypes occupy a region on chromosome (Chr) 17 which slightly overlaps the ends of the T-H-2 interval. The wild-type form of this 14 centi-Morgan (cM) region was mapped in a multilocus backcross (C57BL/10-T x C3H)F1 x C57BL/10 using 15 DNA probes on Southern blots of the DNA extracted from 53 animals which were recombinants in the T-H-2 interval. Each recombinant was also progeny-tested to ascertain its Hybrid sterility-1 (Hst-1) genotype by crossing to PWB/Ph, a Mus musculus-derived inbred strain. The limit of resolution of the cross was 0.27 cM. The map distances have been determined for the DNA loci in the T-H-2 interval and the Hst-1 gene was mapped in close vicinity to the D17Rp17 locus.
Forejt
J
J
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
Vincek
V
V
Klein
J
J
Lehrach
H
H
Loudová-Micková
M
M
D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D015342:DNA Probes; D005260:Female; D006183:H-2 Antigens / Q000235:genetics; D006824:Hybridization, Genetic / Q000235:genetics*; D007246:Infertility / Q000235:genetics*; D008297:Male; D051379:Mice / Q000235:genetics*; D008817:Mice, Mutant Strains
D015342:DNA Probes; D006183:H-2 Antigens