Fast and simple anion-exchange chromatography for large-scale purification of self-complementary oligonucleotides.
A fast and simple anion-exchange chromatography method is described for large-scale purification of synthetic oligonucleotides. Using a single matrix and aqueous solvent system, the two-step chromatographic procedure can handle complex separation problems of self-complementary or G-rich sequences without the use of urea or formaldehyde. The work also demonstrates the complication encountered, possibly due to hairpin formation, in one of the oligomers.
Banerjee
A
A
Jawaharlal Nehru University, New Delhi, India.
Bose
H S
HS
Roy
K B
KB
D001426:Bacterial Proteins; D001483:Base Sequence; D002852:Chromatography, Ion Exchange / Q000379:methods*; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000302:isolation & purification*; D012685:Sepharose
D001426:Bacterial Proteins; D009838:Oligodeoxyribonucleotides; D012685:Sepharose; D015247:Deoxyribonuclease HindIII; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific