Alzheimer's disease: a monoclonal antibody raised against paired helical filaments.
Paired helical filaments (PHF) were isolated from the cerebral cortex of patients with Alzheimer's disease (AD) by a combination of SDS treatment and density gradient centrifugation according to the method of Ihara et al. (1983). The protein component of the preparation was extracted with formic acid and Balb/c mice were used for immunization. Hybridoma supernatants were screened by immunocytochemical staining, by an ELISA assay, and by immunoblotting of SDS-PAGE, the latter both using the PHF preparation as antigen. One hybridoma which showed a strong reactivity with PHF in both the ELISA assay and immunocytochemistry was then used to produce ascites fluid in Balb/c mice. Antibodies reacted immunocytochemically with neurofibrillary tangles and neurites involved in plaque formation in AD but did not show a cross-reaction to human control brain and rat brain. The results indicate that the antibody which has been raised reacts with an antigen component of PHF.
Brückner
M
M
Department of Neurochemistry, Paul Flechsig Institute of Brain Research, University of Leipzig, Germany.
Bendix
U
U
Hube
M
M
Arendt
T
T
Bigl
V
V
D000544:Alzheimer Disease / Q000276:immunology* / Q000473:pathology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001202:Ascitic Fluid / Q000276:immunology; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas; D015151:Immunoblotting; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016501:Neurites / Q000276:immunology; D016874:Neurofibrillary Tangles / Q000276:immunology
D000911:Antibodies, Monoclonal