title: Relationship between inositol 1,4,5-trisphosphate mass level and [14C]aminopyrine uptake in gastrin-stimulated parietal cells.
abstract: The relationship between gastrin-stimulated inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) content and [14C]aminopyrine ([14C]AP) uptake (an index of in vitro acid secretion) was investigated in a population of highly enriched rabbit parietal cells (90 +/- 5%). Gastrin induced a rapid rise in Ins(1,4,5)P3 content which was maximal within 15 s of stimulation (2- to 2.5-fold basal level) followed by a rapid decrease within 30 s; a high Ins(1,4,5)P3 level could also be observed after a longer time of hormone stimulation (180 s). Gastrin dose-dependently induced Ins(1,4,5)P3 accumulation and [14C]AP uptake; both dose-response curves were similar (EC50 approximately 0.1 nM). Furthermore, L-365,260 (3-(acylamino)benzodiazepine), a selective gastrin/CCK-B receptor antagonist, dose-dependently inhibited Ins(1,4,5)P3 production and [14C]AP accumulation induced by 10 nM gastrin with a similar potency (IC50 approximately 1-2 nM). These results led us to conclude that Ins(1,4,5)P3 is involved in gastrin-stimulated acid secretory activity of gastric parietal cells.
authors.affiliation: Laboratoire de Biochimie des Membranes, CNRS UPR-8402 - INSERM U-249, Faculté de Pharmacie, Montpellier, France.
authors.forename: S
authors.initials: S
authors.lastname: Roche
authors.forename: T
authors.initials: T
authors.lastname: Gusdinar
authors.forename: J P
authors.initials: JP
authors.lastname: Bali
authors.forename: R
authors.initials: R
authors.lastname: Magous
mesh_terms: D000632:Aminopyrine / Q000378:metabolism*; D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology; D005744:Gastric Acid / Q000378:metabolism; D005755:Gastrins / Q000502:physiology*; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D007700:Kinetics; D010295:Parietal Cells, Gastric / Q000378:metabolism*; D010671:Phenylurea Compounds; D011817:Rabbits
chemical_list: D001570:Benzodiazepinones; D005755:Gastrins; D010671:Phenylurea Compounds; D000632:Aminopyrine; C058121:L 365260; D015544:Inositol 1,4,5-Trisphosphate