The MELANIE project: from a biopsy to automatic protein map interpretation by computer.
The goals of the MELANIE project are to determine if disease-associated patterns can be detected in high resolution two-dimensional polyacrylamide gel electrophoresis (HR 2D-PAGE) images and if a diagnosis can be established automatically by computer. The ELSIE/MELANIE system is a set of computer programs which automatically detect, quantify, and compare protein spots shown on HR 2D-PAGE images. Classification programs help the physician to find disease-associated patterns from a given set of two-dimensional gel electrophoresis images and to form diagnostic rules. Prototype expert systems that use these rules to establish a diagnosis from new HR 2D-PAGE images have been developed. They successfully diagnosed cirrhosis of the liver and were able to distinguish a variety of cancer types from biopsies known to be cancerous.
Appel
R D
RD
Numeric Imaging Group, Geneva University Hospital, Switzerland.
Hochstrasser
D F
DF
Funk
M
M
Vargas
J R
JR
Pellegrini
C
C
Muller
A F
AF
Scherrer
J R
JR
D001706:Biopsy; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005103:Expert Systems; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D009369:Neoplasms / Q000473:pathology; D010449:Peptide Mapping / Q000379:methods*; D012984:Software