A modification of the indirect immunofluorescence test for detection of Ehrlichia phagocytophila antibodies.
A modified technique for production of antigen and performance of the test is described. A suspension of infected neutrophils was directly applied to multiwell slides. Multichannel pipettes may be used for dilution and application of sera. The modification increases the capacity both by production of the antigen and by performance of the test. This paper also gives a quantitative determination of the antibodies.
Hardeng
F
F
Department of Large Animal Clinical Sciences, Norwegian College of Veterinary Medicine, Oslo.
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology; D004536:Ehrlichia / Q000276:immunology*; D016873:Ehrlichiosis / Q000276:immunology / Q000662:veterinary*; D005260:Female; D005455:Fluorescent Antibody Technique; D015203:Reproducibility of Results; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial