title: Structural and genetic analysis of electrostatic and other interactions in bacteriophage T4 lysozyme.
abstract: The lysozyme from bacteriophage T4 is being used as a model system to determine the roles of individual amino acids in the folding and stability of a typical globular protein. Such studies can provide quantitative information on the contributions made by different types of interactions including hydrogen bonds, hydrophobic interactions, salt bridges and disulphide bridges. To determine the contribution of long-range electrostatic interactions a combination of charge-change mutations was used to reduce the overall formal charge on T4 lysozyme at neutral pH from +9 to +1 units. Such changes in charge were found to have little effect on the stability of the molecule. Salt bridges engineered on the surface of the protein also were found to contribute little to stability. In contrast, the introduction of acidic groups designed to interact with the partial positive charges at the N-termini of alpha-helices consistently increased the stability of the protein. It is argued that this difference between electrostatic salt-bridge interactions and electrostatic 'helix-dipole' interactions lies in the entropic cost of bringing together the interacting partners. In an attempt to simplify the folding problem, and also to further investigate the helix propensity of different amino acids, a series of alanines was introduced within an alpha-helix of T4 lysozyme. The resultant protein not only folds normally but is also more stable than the wild-type enzyme, adding further support to recent evidence that alanine is a helix-favouring amino acid.
authors.affiliation: Department of Physics, University of Oregon, Eugene 97403.
authors.forename: S
authors.initials: S
authors.lastname: Dao-pin
authors.forename: H
authors.initials: H
authors.lastname: Nicholson
authors.forename: W A
authors.initials: WA
authors.lastname: Baase
authors.forename: X J
authors.initials: XJ
authors.lastname: Zhang
authors.forename: J A
authors.initials: JA
authors.lastname: Wozniak
authors.forename: B W
authors.initials: BW
authors.lastname: Matthews
mesh_terms: D004560:Electricity; D004795:Enzyme Stability; D008956:Models, Chemical; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry* / Q000235:genetics; D009154:Mutation / Q000235:genetics; D011487:Protein Conformation; D013499:Surface Properties; D013604:T-Phages / Q000201:enzymology* / Q000235:genetics
chemical_list: D009113:Muramidase