Studies of dialysates from Dermatophagoides farinae: partial purification and haptenic properties of dialysates from Dermatophagoides farinae.
Dialysates from Dermatophagoides farinae were partially purified. Fractionation on HPLC and anion exchange chromatography revealed that the dialysates consisted of 5 major fractions of glycoprotein whose apparent molecular sizes were 5.1, 4.1, 3.2, and less than 1.35 kD on HPLC. The apparent molecular size of two fractions was 5.3 and 2.9 kD on SDS-PAGE. They were basic glycoproteins which had a pI ranging from 7.46 to 8.71 on PAG-IEF. These fractions were allergenic in the RAST and ELISA inhibition tests but not in the skin prick test (SPT). Our results suggest that the dialysates from D. farinae have haptenic properties. The dialysates from D. farinae (low molecular weight) and its 5 fractions bound noncovalently to human serum albumin (HSA) at the free tyrosine residues of HSA. They proved to bind noncovalently to serum proteins and collagens. Once they bound to proteins, the conjugates became allergenic not only in the RAST and ELISA inhibition test but also in the SPT. Our results provide evidence that the dialysates from D. farinae have haptenic properties.
Tamura
H
H
Department of Pediatrics, Gunma University, School of Medicine, Showa, Japan.
Mochizuki
H
H
Shigeta
M
M
Arakawa
H
H
Kuroume
T
T
D000361:Agammaglobulinemia / Q000097:blood; D000485:Allergens / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D039741:Antigens, Dermatophagoides; D001665:Binding Sites; D001798:Blood Proteins / Q000378:metabolism; D002648:Child; D003429:Cross Reactions; D006241:Haptens / Q000276:immunology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008925:Mites; D014442:Monophenol Monooxygenase / Q000378:metabolism; D011852:Radioallergosorbent Test; D012709:Serum Albumin / Q000378:metabolism; D014020:Tissue Extracts / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*
D000485:Allergens; D039741:Antigens, Dermatophagoides; D001798:Blood Proteins; D006241:Haptens; D012709:Serum Albumin; D014020:Tissue Extracts; D014442:Monophenol Monooxygenase