Trans-ACPD-induced Ca2+ signals in cerebellar Purkinje cells.
A combination of intracellular recording and fluorometric measurements of cytosolic calcium [( Ca2+]i) was used to locate changes in [Ca2+]i induced by the specific metabotropic glutamate receptor (mGluR) agonist trans-D,L-1-amino-1,3-cyclopentanedicarboxylic acid (t-ACPD), in Purkinje cells of rat cerebellar slices. Under voltage-clamp conditions, application of t-ACPD (100 microM) induced an inward current accompanied by a large increase in [Ca2+]i located primarily in the soma but also, to a lesser degree, in restricted parts of the dendrites. In contrast, elevations of [Ca2+]i associated with calcium spikes were confined to the dendrites and inward currents of a similar amplitude induced by (RS)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), an agonist of ionotropic glutamate receptors, did not raise [Ca2+]i.
Vranesic
I
I
Brain Research Institute, University of Zürich, Switzerland.
Batchelor
A
A
Gähwiler
B H
BH
Garthwaite
J
J
Staub
C
C
Knöpfel
T
T
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003515:Cycloleucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D003712:Dendrites / Q000378:metabolism; D004594:Electrophysiology; D005470:Fluorometry; D009994:Osmolar Concentration; D011689:Purkinje Cells / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D015398:Signal Transduction
D003515:Cycloleucine; C036361:1-amino-1,3-dicarboxycyclopentane; D002118:Calcium