Immunochemical localization of major hydatid fluid antigens in protoscoleces and cysts of Echinococcus granulosus from human origin.
Monospecific rabbit antisera obtained through experimental immunization with previously purified proteins were used in the structural localization of two hydatid fluid antigens, antigen 5 and antigen B, in cyst membranes and protoscoleces of E. granulosus from human origin. The antigen-antibody reaction was revealed by an avidin-biotin-peroxidase technique. Antigen 5 was not evident in the laminated membrane of the cyst wall, but it was associated with the germinal membrane of the cyst wall and brood capsules. The parenchyma of invaginated and evaginated protoscoleces was heavily labelled. The tegument, the calcareous corpuscles, the suckers and the hooks did not contain antigen 5. Degenerated protoscoleces were also labelled. Antigen B localization was essentially identical to antigen 5, but degenerated protoscoleces were not recognized by anti-antigen B antiserum. Technical aspects and differences with previously published work are discussed.
Sánchez
F
F
Servicio de Microbiologia, Hospital de la Sta. Creu i St. Pau, Barcelona, Spain.
March
F
F
Mercader
M
M
Coll
P
P
Muñoz
C
C
Prats
G
G
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000032:analysis*; D004443:Echinococcosis / Q000276:immunology*; D004446:Echinococcus / Q000254:growth & development / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D011817:Rabbits
D000909:Antibodies, Helminth; D000947:Antigens, Helminth