Growth of human immunodeficiency virus I in cultured cells in the absence of the CD4 antigen.
The ability of a variety of epithelial, embryonal, placental, and neuronal cells to express the CD4 antigen and to be infected by human immunodeficiency virus 1 (HIV-1) was examined. Only two (IMR-32 and HeLa-T4) expressed CD4 detectable by indirect immunofluorescence, and both were infectable by HIV-1. Two others, a human laryngeal carcinoma (HEp-2) and human colonic carcinoma (HT-29), did not express CD4 antigen but were infectable by HIV-1. Infection of the HEp-2 cells was detectable four months (and 20 serial passages) later. Infection of HEp-2 cells was not inhibited by anti CD4 monoclonal antibody but was by the lectin concanavalin A. These results suggest the presence of a receptor other than CD4 can be involved in HIV-1 infection.
Morrison
N K
NK
Department of Medical Microbiology, University of Liverpool, United Kingdom.
McCarthy
K
K
Hart
C A
CA
D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D015704:CD4 Antigens; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D015497:HIV-1 / Q000254:growth & development* / Q000276:immunology / Q000502:physiology; D006801:Humans; D015728:Receptors, HIV / Q000276:immunology; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D015728:Receptors, HIV