title: Biochemical and cytological evidence for an overabundance of mucocysts in the bcd pattern mutant of Tetrahymena thermophila.
abstract: Three acidic proteins (42 kD, 43 kD and 50 kD) were present in unusually high concentrations in cortical preparations of the Tetrahymena pattern mutant broadened cortical domains (bcd). Antisera to the 42-kD and 50-kD proteins bound to discharging mucocysts and food vacuole contents in both wild-type and mutant cells. Subsequent analysis revealed that bcd mutant cell pellicles possess five times more "docked" mucocysts than their wild-type counterparts.
authors.affiliation: Biology Department, University of Iowa, Iowa City 52242.
authors.forename: E S
authors.initials: ES
authors.lastname: Cole
authors.forename: K R
authors.initials: KR
authors.lastname: Stuart
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000918:Antibody Specificity; D002458:Cell Fractionation; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D009154:Mutation; D015388:Organelles; D010641:Phenotype; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D016808:Tetrahymena thermophila / Q000737:chemistry / Q000166:cytology / Q000235:genetics*
chemical_list: D000913:Antibodies, Protozoan; D015800:Protozoan Proteins