In vivo and in vitro inactivation of bovine surfactant by an anti-surfactant monoclonal antibody.
In this study the importance of a low-weight surfactant protein (11 kDa) is demonstrated by selectively blocking this protein with a monoclonal antibody. In adult rats respiratory failure was induced by repeated bronchoalveolar lavage to remove all pulmonary surfactant. It was shown that surfactant mixed with the antibody was not capable of restoring lung function when compared with surfactant alone or surfactant mixed with control serum. Using the pulsating bubble surfactometer, it could be demonstrated that surfactant mixed with this antibody had a significant higher minimum surface tension when compared with surfactant alone, or surfactant mixed with an unrelated mouse immunoglobulin G (IgG). The inhibition of surfactant function by the monoclonal antibody suggests the importance of the 11 kDa protein for normal surfactant function.
Eijking
E P
EP
Dept of Anesthesiology, Erasmus University, Rotterdam, The Netherlands.
Strayer
D S
DS
van Daal
G J
GJ
Tenbrinck
R
R
Merritt
T A
TA
Hannappel
E
E
Lachmann
B
B
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D066298:In Vitro Techniques; D008168:Lung / Q000473:pathology; D010100:Oxygen / Q000097:blood; D011659:Pulmonary Gas Exchange; D011663:Pulmonary Surfactants / Q000276:immunology*; D051381:Rats; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains; D012131:Respiratory Insufficiency / Q000503:physiopathology
D000911:Antibodies, Monoclonal; D011663:Pulmonary Surfactants; D010100:Oxygen