title: Demonstration of immuno-bound alkaline phosphatase by a direct lead method. Optimization of reaction parameters by microdensitometry using computer-aided image processing.
abstract: Antigens labelled by the immunohistochemical alkaline phosphatase anti-alkaline phosphatase (APAAP) method can be visualized by a lead capture technique. Optimal conditions of the reaction, evaluated by microdensitometry using computer-aided image processing, are described.
authors.affiliation: Fachrichtung 3.1 Anatomie im Fachbereich Theoretische Medizin, Universität des Saarlandes, Homburg/Saar, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Bock
authors.forename: K
authors.initials: K
authors.lastname: Chakraverty-Werner
authors.forename: G
authors.initials: G
authors.lastname: Herth
authors.forename: N
authors.initials: N
authors.lastname: Liu
authors.forename: E
authors.initials: E
authors.lastname: Ostermann
authors.forename: U
authors.initials: U
authors.lastname: Töx
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D000906:Antibodies; D003720:Densitometry / Q000379:methods; D007031:Hypothalamus / Q000166:cytology* / Q000201:enzymology; D007150:Immunohistochemistry / Q000379:methods; D009412:Nerve Fibers / Q000201:enzymology / Q000648:ultrastructure*; D009481:Neurophysins / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014667:Vasopressins / Q000032:analysis
chemical_list: D000906:Antibodies; D009481:Neurophysins; D014667:Vasopressins; D000469:Alkaline Phosphatase