The combined treatment of human peripheral blood mononuclear cells with thymolymphotropin and interleukin 2 increases PPD-driven T-cell proliferation and IL-2 induced cellular cytotoxicity against HIV-infected cells.
Two in vitro systems (the DNA synthetic response to mycobacterial antigens and cytotoxicity against lymphoid cells) were used to analyse the effect of thymolymphotropin (TLT) on peripheral blood mononuclear cells (PBMC). Purified protein derivative of mycobacteria (PPD)-driven T-cell proliferation in low-responder donors was increased by the combined treatment with TLT and suboptimal doses of recombinant interleukin 2 (IL-2). Similarly, the activities of natural killer (NK) cells and lymphokine-activated killer (LAK) cells have been enhanced in PBMC cultures pretreated with TLT. Also, TLT showed an enhancing effect on the development of LAK cells capable of lysing Epstein-Barr virus (EBV)-transformed B-lymphocytes infected or uninfected with the human immunodeficiency virus (HIV).
Di Massimo
A M
AM
Department of Biology, II University of Rome, Italy.
Gilardini Montani
M S
MS
Gilardini
M S
MS
Bardone
M R
MR
Moras
M L
ML
Malkovsky
M
M
Antonelli
G
G
Colizzi
V
V
D003602:Cytotoxicity, Immunologic; D006678:HIV / Q000502:physiology; D015658:HIV Infections / Q000276:immunology / Q000628:therapy; D006801:Humans; D007167:Immunotherapy; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000008:administration & dosage*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000276:immunology; D013949:Thymus Extracts / Q000008:administration & dosage*; D014373:Tuberculin / Q000276:immunology; D014779:Virus Replication
D007376:Interleukin-2; D013949:Thymus Extracts; D014373:Tuberculin; C051006:thymomodulin