Use of an established cell line in the evaluation of the cytotoxic effects of various chemicals.
The HTC hepatoma cell line was used as an "in vitro" model to detect the cytotoxicity of eighteen chemicals, chosen on the basis of different biological activities and physicochemical characteristics. Two different cytotoxicity assays measuring cell lethality (CS) or inhibition of cell growth (CF) were applicated to confluent cell monolayers or to colony-forming cells, respectively. Cells were exposed to the chemicals at doses ranging from 10(-6) M to 10(-2) M for 24 h. The results indicated a wide range of IC 50 (the concentration resulting in 50% inhibition of toxicity parameters) from as low as 1 microM (Potassium dichromate) to as high as 407.5 mM (Ethanol), the sensitivity of the CF test being greater than that of the CS test. A battery of cytotoxicity tests could be established in order to offer simple, rapid and economic methods which can be complementary and, in part, alternative to the use of laboratory animals.
Bassi
A M
AM
Institute of General Pathology, University of Genoa.
Piana
S
S
Penco
S
S
Bosco
O
O
Brenci
S
S
Ferro
M
M
D000826:Animal Testing Alternatives; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004351:Drug Resistance; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D051381:Rats; D012680:Sensitivity and Specificity; D014116:Toxicology / Q000379:methods*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014410:Tumor Stem Cell Assay