Use of N-acetylpsychosine as internal standard for quantitative high-performance liquid chromatographic analysis of glycosphingolipids.
The use of N-acetylpsychosine as an internal standard for the quantitative high-performance liquid chromatography (HPLC) of p-nitrobenzoyl derivatives of glycosphingolipids is described. It is suitable because the chromogen reacts on equimolar basis with both N-acetylpsychosine and sample glycosphingolipids. The use of N-acetylpsychosine as an internal standard was validated by determining the glycosphingolipid content of a system of metastatic variants selected from a murine fibrosarcoma line (T3 cells). Reproducible results were obtained throughout several quantitative analyses of cellular glycosphingolipids and it was possible to determine the glycosphingolipid content of as few as 5 x 10(6) cells.
Cecconi
O
O
Istituto di Patologia Generale, Università degli Studi di Firenze, Florence, Italy.
Ruggieri
S
S
Mugnai
G
G
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005354:Fibrosarcoma / Q000737:chemistry / Q000473:pathology; D006028:Glycosphingolipids / Q000032:analysis*; D051379:Mice; D011609:Psychosine / Q000031:analogs & derivatives; D012015:Reference Standards; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000473:pathology
D006028:Glycosphingolipids; D011609:Psychosine; C072822:N-acetylpsychosine