Protease-catalyzed peptide synthesis in frozen aqueous systems: the "freeze-concentration model".
Freezing of the aqueous reaction mixtures can cause dramatically increased peptide yields in protease-catalyzed kinetically controlled peptide synthesis reactions. The "Freeze-concentration model" which implies that the enzymic reaction within a macroscopically frozen system takes place in an unfrozen liquid phase is presented as a reasonable basis for the understanding of the effect of freezing. It is shown that L-amino acid esters are effective nucleophiles in alpha-chymotrypsin-catalyzed acyl transfer reactions when these are performed in frozen state.
Schuster
M
M
Department of Biochemistry, Leipzig University, FRG.
Aaviksaar
A
A
Haga
M
M
Ullmann
U
U
Jakubke
H D
HD
D000595:Amino Acid Sequence; D002918:Chymotrypsin / Q000737:chemistry; D004151:Dipeptides / Q000138:chemical synthesis / Q000737:chemistry; D010450:Endopeptidases / Q000737:chemistry*; D005615:Freezing; D007700:Kinetics; D008956:Models, Chemical; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D014867:Water
D004151:Dipeptides; D010455:Peptides; D014867:Water; D010450:Endopeptidases; D002918:Chymotrypsin