Purification and characterization of hepatic microsomal cytochrome P-450 in phenobarbital- and beta-naphthoflavone-treated pigs.
Different cytochrome P-450 isoenzymes from hepatic microsomes of phenobarbital (PB) and beta-naphthoflavone (beta-NF) treated pigs and rats were isolated, purified, and characterized. The physico-chemical properties of the porcine isoenzymes were similar to properties of forms isolated from other species. The molecular sizes ranged from 52.5 to 59.5 kD and, in the ferrous-carbonyl state, the isoenzymes had absorbance maxima between 447 and 451 nm. Antigenic similarities were found between the isoenzymes present in PB-induced pigs, and between the isoenzymes present in beta-NF-induced pigs. Cross-reactivity was not observed between PB- and beta-NF-inducible isoenzymes, but beta-NF-inducible isoenzymes in pigs and rats possessed antigenic similarities.
Thomsen
M K
MK
Department of Pharmacology, Leo Pharmaceutical Products, Ballerup, Denmark.
Friis
C
C
Nielsen
P
P
D000818:Animals; D001571:Benzoflavones / Q000494:pharmacology*; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D005260:Female; D005779:Immunodiffusion; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D008970:Molecular Weight; D009929:Organ Size; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013552:Swine; D019324:beta-Naphthoflavone
D001571:Benzoflavones; D007527:Isoenzymes; D019324:beta-Naphthoflavone; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital