title: [Studies on double-labelling method for determination of T lymphocyte subpopulations].
abstract: In order to determine the concurrent expression of DR and CD antigen of T lymphocyte surface markers, the fluorescence-rosette double-labelling technique was developed by employing monoclonal antibody against HLA-DR and sensitized sheep erythrocytes with OKT series of monoclonal antibodies against cell surface antigen. Its reliability was evaluated by comparison with two-step isolating technique for determining DR antigen of activated T lymphocyte subsets in 30 healthy volunteers. The result showed there was no statistic difference between results determined with two methods. The double-labelling technique was characterized by its samplity, no injury for cellular activity and ability for analysing the expression of the single antigen and double antigens on cell surface concurrently.
authors.affiliation: Research Center of Experimental Medicine, Tongji Medical University, Wuhan.
authors.forename: G X
authors.initials: GX
authors.lastname: Shen
authors.forename: W J
authors.initials: WJ
authors.lastname: Dai
authors.forename: X L
authors.initials: XL
authors.lastname: Wang
authors.forename: N
authors.initials: N
authors.lastname: Su
authors.forename: H F
authors.initials: HF
authors.lastname: Zhu
authors.forename: W X
authors.initials: WX
authors.lastname: Ye
authors.forename: J W
authors.initials: JW
authors.lastname: Li
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D005455:Fluorescent Antibody Technique; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D012397:Rosette Formation; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens
vernacular_title: Studie über ein Doppelmarkierungsverfahren zur Bestimmung von T-Lymphozytensubpopulationen.