title: A soluble fumarate reductase in Trypanosoma brucei procyclic trypomastigotes.
abstract: The enzyme NADH-fumarate reductase associated with the membrane fraction of Trypanosoma brucei procyclic trypomastigotes, can be solubilized by more than 50% when increasing the ionic strength to the equivalent of 150 mM KCl. The apparent KMs for NADH (125 microM) and fumarate (50 microM) remain close to those previously reported for the membrane-bound form of this enzyme. Other electron acceptors (i.e. oxygen or cytochrome c) appear to accept electrons in the absence of fumarate (KM for cytochrome c = 50 microM). The drug L-092,201 (Merck, Sharp and Dohme Research Laboratories, Rahway, NJ), an inhibitor of the membrane-bound fumarate reductase, also blocked the solubilized enzyme. Given the relatively high ionic strength of the intracellular environment we propose that, in vivo, the enzyme fumarate reductase is in the mitochondrial matrix or in the soluble fraction of another intracellular compartment.
authors.affiliation: Department of Biochemistry, College of Medicine, University of South Alabama, Mobile 36688.
authors.forename: J
authors.initials: J
authors.lastname: Mracek
authors.forename: S J
authors.initials: SJ
authors.lastname: Snyder
authors.forename: U B
authors.initials: UB
authors.lastname: Chavez
authors.forename: J F
authors.initials: JF
authors.lastname: Turrens
mesh_terms: D000818:Animals; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000201:enzymology; D011189:Potassium Chloride; D013379:Substrate Specificity; D013385:Succinate Dehydrogenase / Q000032:analysis* / Q000037:antagonists & inhibitors; D014346:Trypanosoma brucei brucei / Q000201:enzymology*
chemical_list: D011189:Potassium Chloride; D013385:Succinate Dehydrogenase