Detection of Clostridium difficile from an enhancement broth by gas-liquid chromatography.
Gas-Liquid chromatography (GLC) was used to detect the presence of isocaproic acid produced by Clostridium difficile from 54 stool samples grown in cycloserine-cefoxitin broth. Isocaproic acid was detected in 12 samples of which 5 were confirmed to be Clostridium difficile by culture and biochemical tests. The detection of isocaproic acid by GLC together with the presence of presumptive colonies on primary selective culture media provides a more rapid laboratory diagnosis for Clostridium difficile.
Hassan
H
H
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur.
Cheng
S C
SC
D002208:Caproates / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods; D016360:Clostridioides difficile / Q000302:isolation & purification* / Q000378:metabolism; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D006801:Humans; D013997:Time Factors
D002208:Caproates; C034527:isocaproic acid