title: Role of the zymolyase-sensitive cyst wall of Pneumocystis carinii in the oxidative burst of macrophages.
abstract: Alveolar macrophages are thought to participate in clearing Pneumocystis carinii (Pc) from the lungs. We have recently demonstrated that Pc cysts and trophozoites induce an oxidative burst in a cell line of rat alveolar macrophages (NR8383). In order to investigate the mechanism of this response, we examined the effect that disruption of the Pc cyst wall with zymolyase had on the cyst's ability to elicit H2O2 from NR8383 macrophages and correlated these results with the electron microscopic appearance of the cyst wall.
authors.affiliation: University of Texas Health Science Center, Department of Pediatrics, San Antonio 78284-7815.
authors.forename: H A
authors.initials: HA
authors.lastname: Hidalgo
authors.forename: R J
authors.initials: RJ
authors.lastname: Helmke
authors.forename: V F
authors.initials: VF
authors.lastname: German
authors.forename: J A
authors.initials: JA
authors.lastname: Mangos
mesh_terms: D000818:Animals; D002473:Cell Wall / Q000276:immunology* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008297:Male; D011010:Pneumocystis / Q000276:immunology* / Q000648:ultrastructure; D011020:Pneumonia, Pneumocystis / Q000276:immunology; D051381:Rats; D016897:Respiratory Burst
chemical_list: C037833:zymolyase; D006861:Hydrogen Peroxide; D006867:Hydrolases