Kinetic analysis of smooth muscle relaxation.
A method has been proposed for the kinetic analysis of the relaxation phase of mechanical response of smooth muscle. The method implies linearization of the entire mechano-kinetic relaxation curve using the coordinates ln[(fm-f)/f]; ln t (where fm and f are the maximal and the actual values of force within the relaxation phase respectively) with subsequent calculation of the maximal amplitude--normalized relaxation rate, Vn. The use of the method is illustrated on ureter, protal vein, vas deferens, myometrium of rat and guinea-pig ureter smooth muscles in a variety of experimental conditions. This method can be applied for the description of the calcium transient decay in the myoplasm measured with Ca-sensitive fluorescent dyes. The method might be useful for identification of the contribution of different energy-dependent Ca-transporting systems involved in the control of relaxation, as well as for screening of the mechanisms of action of different factors which modulate the contraction-relaxation cycle.
Burdyga
V
V
Department of Biochemical Kinetics, A.V. Palladine Institute of Biochemistry, Academy of Sciences of the Ukrainian S.S.R., Kiev.
Kosterin
S A
SA
D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism; D005260:Female; D006168:Guinea Pigs; D007700:Kinetics; D008297:Male; D009119:Muscle Contraction / Q000502:physiology; D009126:Muscle Relaxation / Q000502:physiology*; D009130:Muscle, Smooth / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000502:physiology; D009215:Myometrium / Q000502:physiology; D011169:Portal Vein; D051381:Rats; D013696:Temperature; D014521:Urethra; D014649:Vas Deferens
D002118:Calcium