title: Evaluation of a rapid dot-ELISA as a field test for the diagnosis of cystic hydatid disease.
abstract: A rapid dot enzyme-linked immunosorbent assay (dot-ELISA) was developed as a field test for the diagnosis of cystic hydatid disease caused by Echinococcus granulosus. The 30 min test was based on the detection of antibodies to antigen B of hydatid fluid and was carried out using 50 microliters of whole blood in a field assessment in the Turkana region of north-west Kenya. Initial laboratory studies showed antigen B to be preferable to crude cyst fluid, with 94% sensitivity and 90.3% specificity for Echinococcus infections. The field test was rapid, inexpensive and simple to perform and is considered to be a useful back-up to ultrasound scanning.
authors.affiliation: Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, UK.
authors.forename: M T
authors.initials: MT
authors.lastname: Rogan
authors.forename: P S
authors.initials: PS
authors.lastname: Craig
authors.forename: E
authors.initials: E
authors.lastname: Zeyhle
authors.forename: T
authors.initials: T
authors.lastname: Romig
authors.forename: G M
authors.initials: GM
authors.lastname: Lubano
authors.forename: L
authors.initials: L
authors.lastname: Deshan
mesh_terms: D000909:Antibodies, Helminth / Q000032:analysis*; D004443:Echinococcosis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007630:Kenya; D013997:Time Factors
chemical_list: D000909:Antibodies, Helminth