title: Accumulation of ascites and increase in skin vascular permeability observed by injection of adsorbed diphtheria-purified pertussis-tetanus combined vaccine in guinea pigs.
abstract: Intraperitoneal injection of adsorbed diphtheria-purified pertussis-tetanus combined vaccine (DPPT) often causes an ascites-accumulating (A-A) reaction in guinea pigs. Those vaccines which caused A-A reaction increase the vascular permeability (VP) of the skin tissue at the intracutaneous injection sites. A total of 23 lots of DPPT were assayed for A-A and VP-increasing activities. A positive correlation between the volume of ascites accumulated and the intensity of VP was significant. Other kinds of aluminum-adsorbed (Al-Ad) vaccines such as Al-Ad tetanus and Al-Ad diphtheria-tetanus combined and Al-Ad hepatitis B vaccines induced little or no A-A reaction. These results were discussed from the viewpoint of quality control in the process of the production of DPPT.
authors.affiliation: Department of General Biologics Control, National Institute of Health, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Goto
authors.forename: H
authors.initials: H
authors.lastname: Kato
mesh_terms: D000279:Administration, Cutaneous; D000536:Aluminum Hydroxide; D000818:Animals; D001201:Ascites / Q000209:etiology* / Q000503:physiopathology; D002199:Capillary Permeability; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000008:administration & dosage* / Q000009:adverse effects / Q000592:standards; D005260:Female; D006168:Guinea Pigs; D007274:Injections, Intraperitoneal; D012867:Skin / Q000098:blood supply* / Q000473:pathology
chemical_list: D015721:Diphtheria-Tetanus-Pertussis Vaccine; D000536:Aluminum Hydroxide