Effects of harmane on growth and in vivo metabolism of aflatoxin B1 in male and female rats.
The role of harmane, a beta-carboline formed during pyrolysis of tryptophan, on the metabolism of AFB1, growth and some parameters of the nutritional status was investigated in the rat. Male and female Wistar rats were fed a semi-synthetic diet containing AFB1 (2 ppm), harmane (250 ppm) or both compounds, for 33 days after weaning. Qualitative and quantitative differences in the urinary and faecal excretion of parental compound and metabolites were assessed by HPLC analysis. Harmane did not modify appreciably the growth and the other nutritional parameters studied. Similar excretion patterns of AFB1 metabolites were observed in males and females. Harmane caused a limited increase in the excretion of AFM1 in faeces but not in urine, without altering the growth process in rats of either sex.
Billaud
C
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Laboratoire de Biochimie Industrielle et Agro-alimentaire, Conservatoire National des Arts et Métiers, Paris, France.
D016604:Aflatoxin B1 / Q000008:administration & dosage / Q000378:metabolism* / Q000493:pharmacokinetics; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D005243:Feces / Q000737:chemistry; D005260:Female; D006247:Harmine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D009752:Nutritional Status / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D015430:Weight Gain / Q000187:drug effects*
D006247:Harmine; C005010:harman; D016604:Aflatoxin B1