Parathyroid hormone (1-34) and Nleu8,18Tyr34-parathyroid hormone, (3-34) amide increase diacylglycerol in neonatal mouse calvaria.
Previously, we and others have presented evidence that a calcium second messenger system is involved in the action of parathyroid hormone (PTH) on bone. In the present report, the effects of PTH(1-34) and PTH(3-34)amide treatment on diacylglycerol (DG) in neonatal mouse calvaria are described. PTH(1-34) produced a rapid (within 5 minutes) increase in calvarial incorporation of 3H-arachidonic acid into DG. The effect was maximal at 0.1 nMPTH(1-34), the lowest concentration tested. The 3-34 amide analogue of PTH increased DG to the same extent as PTH(1-34). The effect was maximal at 10 nM PTH(3-34)amide, the lowest concentration tested. These concentrations were lower than those required to elicit maximal effects on bone resorption. In contrast to effects on cyclic AMP, where the 3-34 amide inhibited the increase elicited by PTH, combined treatment of calvaria with PTH(1-34) and PTH(3-34)amide did not inhibit effects on resorption or diacylglycerol.
Stewart
P J
PJ
Department of Pharmacology, Northwestern University Medical School, Chicago, Illinois.
Stathopoulos
V M
VM
Stern
P H
PH
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001862:Bone Resorption; D004075:Diglycerides / Q000032:analysis / Q000378:metabolism* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D051379:Mice; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D012886:Skull / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D019379:Teriparatide; D013997:Time Factors
D004075:Diglycerides; D010281:Parathyroid Hormone; D010446:Peptide Fragments; D019379:Teriparatide; C025497:parathyroid hormone (3-34) amide, Nle(8,18)-Nle(34)-