[Analysis of the polypeptide profile in insulin preparations using HPLC].
The purity of insulin preparations which is important during clinical administration of this hormone can be assessed in several ways. The authors suggested and tested a method for analysis of the polypeptide composition of insulin preparations which makes quantitative and qualitative evaluation of the insulin and non-insulin character possible, as well as their separation according to the shape and size of biological macromolecules by gel filtration on HPLC equipment. The described procedure makes possible a more detailed analysis of insulin preparations when the method is used in combination with electromigration and immunological estimations.
Streda
M
M
I. interní klinika 1. lékarské fakulty Univerzity Karlovy, Praha.
Stajner
K
K
Stredová
M
M
D002851:Chromatography, High Pressure Liquid; D007328:Insulin / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*
D007328:Insulin; D011506:Proteins
Analýza polypeptidového profilu inzulínových preparátů metodou HPLC.