[The role of changes in the cytoplasmic concentration of Ca2+ in dexamethasone-induced thymocyte death].
The putative role of changes in cytosolic Ca2+ concentration ([Ca2+]i) in the dexamethasone (DM) induced thymocyte death was investigated. Incubation of rat thymocytes with 10(-7) M DM for different time intervals from 0.1 to 8 h did not change the basal [Ca2+]i level ca 100 nM as measured with Ca(2+)-fluorescent probe Quin-2. Ca2+ influx measured by the rate of 45Ca2+ uptake was also just the same in DM treated and control cells. At the same time a 6-8 h incubation of cell suspension with 10(-7) M DM results in significant increase in DNA fragmentation and pyknosis, and a 24 h incubation is associated with the decrease in the percentage of cells not staining with Trypan blue. Thus, the results obtained indicate that 10(-7) M DM induces thymocyte death without any significant and constant [Ca2+]i rise during the first 8 h after hormone application.
Kotelevskaia
S M
SM
Zinchenko
V P
VP
Petruniaka
V V
VV
Korystov
Iu N
IuN
Shaposhnikova
V V
VV
Kruman
I I
II
Gukovskaia
A S
AS
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D003593:Cytoplasm / Q000187:drug effects* / Q000378:metabolism; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors
D003907:Dexamethasone; D004247:DNA; D002118:Calcium
Rol' izmeneniĭ tsitoplazmaticheskoĭ kontsentratsii Ca2+ v indutsiruemoĭ deksametazonom gibeli timotsitov.