Characterization of smooth Brucella lipopolysaccharides and polysaccharides by monoclonal antibodies.
Two mouse monoclonal antibodies (mAb) generated to the M antigen of Brucella melitensis 16M were analysed. Binding profiles of both monoclonals were established by a competitive enzyme-linked immunosorbent assay using chemically defined lipopolysaccharides, O polysaccharides and native hapten polysaccharides from B. melitensis 16M, B. abortus 544 and Yersinia enterocolitica O:9. Using this assay, significant differences in the reactivity of both antibodies were found with A and M antigens from Brucella spp. and the O polysaccharide from Y. enterocolitica O:9. These findings are consistent with the simultaneous expression of A and M epitopes on the lipopolysaccharide of all smooth Brucella strains. Quantitatively similar inhibitory powers were established for the native hapten and O polysaccharide from B. melitensis 16M. However, different behaviour was observed between both antigenic preparations obtained from B. abortus 544. The use of lipopolysaccharide-M-specific mAb in different serological tests instead of polyclonal antisera may be of great practical use for minimizing the risk of the appearance of cross-serological reactions between smooth Brucella strains and Y. enterocolitica O:9.
Vizcaino
N
N
Department of Microbiology, Faculty of Pharmacy, University of Salamanca, Spain.
Chordi
A
A
Fernández-Lago
L
L
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology; D002002:Brucella / Q000276:immunology* / Q000302:isolation & purification; D002006:Brucellosis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D006241:Haptens / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology; D015008:Yersinia enterocolitica / Q000276:immunology
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D006241:Haptens; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial