title: Expression of the zebrafish paired box gene pax[zf-b] during early neurogenesis.
abstract: The paired box-containing (pax) gene family encodes a group of putative transcription factors differentially expressed during embryonic development. In this study, we describe the cloning and expression of a zebrafish gene pax[zf-b], which most probably is a direct homologue to the mouse Pax2 gene. The putative protein encoded by pax[zf-b] contains a paired box, an octapeptide, but no homeobox. However, a region of homology to the N-terminal half of paired-type homeoboxes is detected C-terminal to the pax[zf-b] paired domain. In zebrafish embryos, pax[zf-b] transcripts are first seen during the formation of the neural keel. At 9-10 h of development, two laterally located transverse stripes of cells expressing the gene appear in the rostral 1/3 of the embryo. The two areas subsequently move towards the midline and form the posterior portion of the midbrain. In the following stages of development, at 10-12 h, transcripts are detected in the otic placode, the Wolffian duct including the nephritic primodium and in the optic stalk. At a later time point, beginning at 14-15 h, single cells along the spinal cord, presumably interneurons, start to express the gene. The characteristic expression pattern of pax[zf-b] in the neural tube suggests an involvement of this gene in the regionalization of the midbrain as well as in the specification of neuronal cell fates at early embryonic stages.
authors.affiliation: Department of Microbiology, University of Tromsø, Norway.
authors.forename: S
authors.initials: S
authors.lastname: Krauss
authors.forename: T
authors.initials: T
authors.lastname: Johansen
authors.forename: V
authors.initials: V
authors.lastname: Korzh
authors.forename: A
authors.initials: A
authors.lastname: Fjose
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004625:Embryo, Nonmammalian / Q000648:ultrastructure; D015870:Gene Expression / Q000502:physiology*; D005796:Genes / Q000235:genetics*; D007668:Kidney / Q000196:embryology; D008854:Microscopy, Electron; D015336:Molecular Probe Techniques; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics*; D009420:Nervous System / Q000196:embryology* / Q000648:ultrastructure; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*