Influence of age on transmembrane potential and cell surface charge of human peripheral blood lymphocytes estimated with fluorescent probes.
Transmembrane potential (TMP) and surface charge (CSC) of human peripheral blood lymphocytes were estimated in relation to donor's age. Cyanine dye DiOC(6) and fluorescein--coupled poly-L-ornithine EPLO were used as representative fluorescent probes for microfluorimetric determination of relative TMP and CSC values. Significant decrease in cyanine fluorescence was observed in lymphocytes of old people, which was interpreted as relative depolarization of these cells. No such difference was found when surface charges of young and old cells were compared, although some brightly fluorescent, i.e. highly charged cells appeared among the latter.
Witkowski
J M
JM
Department of Histology, Medical Academy of Gdańsk, Poland.
Myśliwski
A
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D003592:Cytophotometry; D005260:Female; D019793:Fluorescein; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D008214:Lymphocytes / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D008564:Membrane Potentials / Q000502:physiology*
D005452:Fluoresceins; D005456:Fluorescent Dyes; D019793:Fluorescein