[Anti-invasive and anti-metastatic effect of lysine oxidase from Trichoderma sp. in vitro and in vivo].
A new fungal strain, Trichoderma sp., discovered in Moscow, produces the antitumor enzyme, lysine-oxidase, which demonstrates an anti-invasive effect in vitro and anti-metastatic activity in vivo. Maximal inhibition of the in vitro invasion of MM1 clone cells was obtained when the tumor cells were pretreated with 2.5 mU/ml of lysine-oxidase; the pretreatment caused a 1.9-times reduction in cell growth and a 1.6-times reduction in the invasive capacity. We studied its anti-metastatic effect on the spreading Lewis lung carcinoma (3LL) in mice from which the primary tumor had been removed. The administration of the enzyme (50 U/kg, i.v.) significantly decreased not only the extent but the number of lung metastases, as compared with the untreated mice. In addition to that, the lysine-oxidase treatment considerably increases the life-span of mice from which the primary tumor had been removed (200 days after 3LL implantation, lysine-oxidase treatment caused surviving of 50% mice in experimental group).
Khaduev
S Kh
SKh
Department of Tumor Biochemistry, Center for Adult Diseases, Osaka, Japan.
Umanskiĭ
V Iu
VIu
Vesa
V S
VS
Sinkaĭ
K
K
Akedo
Kh
Kh
Berezov
T T
TT
D000594:Amino Acid Oxidoreductases / Q000627:therapeutic use*; D000818:Animals; D051379:Mice; D008807:Mice, Inbred BALB C; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology; D014242:Trichoderma / Q000201:enzymology*
D000594:Amino Acid Oxidoreductases; C041123:L-lysine oxidase
Issledovanie antiinvazivnogo i antimetastaticheskogo deĭstviia lizinoksiday iz Trichoderma sp. in vitro i in vivo.