[The choice of a method of isolating human IgG F(ab)2 fragments for determining antibodies to staphylococcal protein A].
The comparative study of two methods of the proteolysis of IgG with the aim of obtaining F(Ab)2-fragments from the blood sera of patients and healthy donors has been made. Different methods for the isolation of F (ab)2-fragments, such as gel filtration on Sephadex G-200, affinity chromatography on protein A-BrCN-sepharose, reprecipitation with zinc sulfate, have been analyzed. For further work the method of the peptic decomposition of whole serum with subsequent salting out with ammonium sulfate and gel filtration on Sephadex G-200 has been chosen.
Minster
A B
AB
Perel'man
E V
EV
Vulk
V F
VF
Susova
O V
OV
D000907:Antibodies, Bacterial / Q000097:blood*; D001782:Blood Donors; D002850:Chromatography, Gel / Q000379:methods; D005069:Evaluation Studies as Topic; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000097:blood / Q000302:isolation & purification*; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification*; D007158:Immunologic Techniques; D013203:Staphylococcal Infections / Q000276:immunology; D013205:Staphylococcal Protein A / Q000276:immunology*
D000907:Antibodies, Bacterial; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A
Vybor metoda polucheniia F(ab)2-fragmentov IgG cheloveka dlia opredeleniia antitel k stafilokokkovomu proteinu A.