title: Trehalose-transporting membrane vesicles from yeasts.
abstract: We have isolated and characterized a membrane vesicle fraction from yeasts that is capable trehalose transport. The kinetics of the transport system were similar to those seen in the intact cells. The transport depends on a transmembrane pH gradient. If the gradient is collapsed, trehalose accumulated inside the vesicles is leaked into the medium. After aging for several days the ability of the vesicles to transport was lost. However, transport was partially restored by elevating internal pH in the vesicles.
authors.affiliation: Department of Biochemistry, University of Sao Paulo, Brazil.
authors.forename: P S
authors.initials: PS
authors.lastname: De Araujo
authors.forename: A C
authors.initials: AC
authors.lastname: Panek
authors.forename: J H
authors.initials: JH
authors.lastname: Crowe
authors.forename: L M
authors.initials: LM
authors.lastname: Crowe
authors.forename: A D
authors.initials: AD
authors.lastname: Panek
mesh_terms: D001692:Biological Transport; D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism*; D014199:Trehalose / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D014199:Trehalose