Investigation of products arising from enzymatic digestion of advanced glycated albumin by high-performance liquid chromatography/mass spectrometry.
The structural investigation of the products arising from 28 days incubation of albumin with high glucose concentration and further enzymatic hydrolysis has been carried out by means of high-performance liquid chromatography/mass spectrometry (HPLC/MS) under plasmaspray conditions. By this approach many different compounds have been detected, and for most of them, possible structures have been proposed on the basis of literature data and molecular weight assignments.
Lapolla
A
A
Istituto di Medicina Interna, Patologia Medica 1, Policlinico Universitario, Padova, Italy.
Gerhardinger
C
C
Baldo
L
L
Crepaldi
G
G
Fedele
D
D
Porter
C J
CJ
Seraglia
R
R
Favretto
D
D
Traldi
P
P
D000818:Animals; D002417:Cattle; D010450:Endopeptidases; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D017127:Glycation End Products, Advanced; D006868:Hydrolysis; D066298:In Vitro Techniques; D012709:Serum Albumin / Q000737:chemistry*; D000093423:Glycated Serum Albumin
D017127:Glycation End Products, Advanced; D012709:Serum Albumin; D010450:Endopeptidases; D000093423:Glycated Serum Albumin