Isolation and characterization of a major allergen from oriental mustard seeds, BrajI.
A 2 S albumin from oriental mustard (Brassica juncea) seeds has been isolated and characterized as an allergen. This protein, BrajI, was recognized by human IgE from mustard-sensitive individuals, as proved by using two different enzyme immunoassays. BrajI was found to be closely related to Sin a I, the major allergen from yellow mustard seeds. Many fractions with molecular weights ranging from 16,000 to 16,400 and with differences in charge were separated by ion-exchange chromatography. They exhibited small but significant amino acid composition differences for Glx, Val, Ile, Lys, and Arg contents. The heterogeneity of BrajI can be explained by size and charge differences of its heavy and light chains. All of the isoallergenic forms of BrajI gave a single precipitation band in double diffusion immunoassays when using a Sin a-I-specific rabbit polyclonal serum.
González de la Peña
M A
MA
Departamento de Bioquímica y Biología Molecular I, Facultad de Ciencias Químicas, Universidad Complutense, Madrid, Spain.
Menéndez-Arias
L
L
Monsalve
R I
RI
Rodríguez
R
R
D055516:2S Albumins, Plant; D000418:Albumins / Q000737:chemistry* / Q000276:immunology; D000485:Allergens / Q000737:chemistry* / Q000276:immunology; D000596:Amino Acids / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000276:immunology; D052179:Antigens, Plant; D001937:Brassica / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000378:metabolism; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry / Q000276:immunology*
D055516:2S Albumins, Plant; D000418:Albumins; D000485:Allergens; D000596:Amino Acids; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; C073840:Bra j protein, Brassica juncea; D010940:Plant Proteins; D007073:Immunoglobulin E