title: Determination of terfenadine and terfenadine acid metabolite in plasma using solid-phase extraction and high-performance liquid chromatography with fluorescence detection.
abstract: This work describes the methodology for the analysis of terfenadine and the acid metabolite of terfenadine in plasma using high-performance liquid chromatography. The use of solid-phase extraction allows the use of robotic or manual sample preparation for the efficient clean-up of terfenadine and terfenadine acid metabolite from plasma. Additional selectivity is obtained through the use of fluorescence detection. For terfenadine, the validated quantitation range of this method is 10.0-84.2 ng/ml with coefficients of variation of 5.7-30%. For terfenadine acid metabolite, the validated quantitation range of this method is 8.2-500 ng/ml with coefficients of variation of 4.1-24%.
authors.affiliation: Marion Merrell Dow Inc., Cincinnati, OH 45215-6300.
authors.forename: J E
authors.initials: JE
authors.lastname: Coutant
authors.forename: P A
authors.initials: PA
authors.lastname: Westmark
authors.forename: P A
authors.initials: PA
authors.lastname: Nardella
authors.forename: S M
authors.initials: SM
authors.lastname: Walter
authors.forename: R A
authors.initials: RA
authors.lastname: Okerholm
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D016593:Terfenadine / Q000031:analogs & derivatives* / Q000097:blood* / Q000378:metabolism
chemical_list: D016593:Terfenadine; C073396:4-(1-hydroxy-4-(4-(hydroxydiphenylmethyl)-1-piperidinyl)butyl)-alpha,alpha-dimethylbenzeneacetic acid