title: Genetic factors that regulate cytosolic epoxide hydrolase activity in normal human lymphocytes.
abstract: To determine whether genetic mechanisms control large variations in cytosolic epoxide hydrolase (cEH) activity of unstimulated lymphocytes from normal human subjects, cEH activity was measured in: a) 6 sets of monozygotic (MZ) twins and 6 sets of dizygotic (DZ) twins; b) 100 unrelated male subjects and c) 6 families. The twin study revealed predominantly genetic control (H2/1 = 0.95). Variability was markedly less within MZ (intrapair variance = 0.25) than DZ twins (intrapair variance = 6.33). In 100 unrelated male subjects the extent of interindividual variation was 11-fold. Unimodal distribution of values among 99 subjects encompassed a six-fold range. One outlier with very high activity clearly stood apart. Using the whole distribution curve we phenotyped members of 6 families. In the outlier's family, analysis of 3 generations suggested autosomal dominant transmission of high cEH activity. Analysis of the other 5 families and 12 sets of twins, all from the large unimodal distribution, was consistent with either monogenic or polygenic control of variations within this mode.
authors.affiliation: Department of Pharmacology, Pennsylvania State University, College of Medicine, Hershey 17033.
authors.forename: E S
authors.initials: ES
authors.lastname: Vesell
mesh_terms: D003600:Cytosol / Q000201:enzymology*; D004851:Epoxide Hydrolases / Q000235:genetics*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000502:physiology*; D008297:Male; D010375:Pedigree; D012016:Reference Values; D015203:Reproducibility of Results; D014429:Twins, Dizygotic / Q000235:genetics; D014430:Twins, Monozygotic / Q000235:genetics
chemical_list: D004851:Epoxide Hydrolases