Prenatal ontogeny of growth hormone releasing hormone expression in rat hypothalamus.
The prenatal development of growth hormone-releasing hormone (GHRH) gene expression was studied in rat hypothalamus using hybridization histochemistry. Two populations of neurons expressing the GHRH gene were found and they had a different pattern of development. Neurons in the arcuate nucleus first expressed GHRH on E16 and 4 days later an adult-like pattern was found. Few neurons along the laterodorsal aspect of the ventromedial hypothalamic nucleus expressed GHRH on E17, with a subsequent increase of expression over the next days. On the day before birth, adult-like patterns were seen. These results demonstrate the differential regulation of GHRH gene expression in the rat hypothalamus.
Burgunder
J M
JM
Neuromorphologisches Labor, Universität Bern, Switzerland.
D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D013007:Growth Hormone-Releasing Hormone / Q000096:biosynthesis* / Q000235:genetics; D006651:Histocytochemistry; D007031:Hypothalamus / Q000254:growth & development / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014697:Ventromedial Hypothalamic Nucleus / Q000254:growth & development / Q000378:metabolism
D012333:RNA, Messenger; D013007:Growth Hormone-Releasing Hormone