title: Acetylcholine modulates the effect of ovarian steroids on glutamic acid decarboxylase activity in the rat fallopian tube.
abstract: Glutamic acid decarboxylase (GAD) activity was measured in the oviduct of normal rats in diestrous and in rats ovariectomized (OVX) seven days before. OVX induced a significant decrease of GAD activity in the Fallopian tube. This effect was completely reversed by coadministration of estradiol benzoate + progesterone (E + P). Simultaneous injection of atropine, but not of alpha-methyl-para-tyrosine or labetalol, completely prevented the activation of GAD induced by ovarian sterois. Moreover, prostigmin significantly potentiated the action of E + P on GAD activity in the rat oviduct. These data clearly suggest the participation of acetylcholine in the mechanisms whereby ovarian steroids regulate GAD activity in the rat Fallopian tube.
authors.affiliation: Instituto de Investigaciones Farmacologicas, CONICET, Argentina.
authors.forename: J A
authors.initials: JA
authors.lastname: Apud
authors.forename: L
authors.initials: L
authors.lastname: Monasterolo
mesh_terms: D000109:Acetylcholine / Q000502:physiology*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001565:Benzoates / Q000494:pharmacology; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D005187:Fallopian Tubes / Q000201:enzymology*; D005260:Female; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D007741:Labetalol / Q000494:pharmacology; D008781:Methyltyrosines / Q000494:pharmacology; D009388:Neostigmine / Q000494:pharmacology; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology*; D011374:Progesterone / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000502:physiology; D013256:Steroids / Q000494:pharmacology*; D019805:alpha-Methyltyrosine
chemical_list: D001565:Benzoates; D008781:Methyltyrosines; D011976:Receptors, Muscarinic; D013256:Steroids; D009388:Neostigmine; D011374:Progesterone; D004958:Estradiol; D019805:alpha-Methyltyrosine; D001285:Atropine; D005968:Glutamate Decarboxylase; D000109:Acetylcholine; D007741:Labetalol