The effect of cisplatin and carboplatin on c-myc promoter in erythroleukemic cells.
The recombinant plasmids p324, p330 and p323 carrying 5' flanking c-myc sequences linked to the reporter gene chloramphenicol acetyl-transferase (cat) were introduced into the mouse erythroleukemia cell line F412B2TK- and stable transfectants resistant to geneticin were obtained. The effects of two platinum coordination compounds, cisplatin and carboplatin, were studied using a wide range of drug concentrations. It was found that cisplatin stimulates cat gene expression with maximum effect at 5 x 10(-5) M concentration, while carboplatin at concentrations from 1 x 10(-6) to 1 x 10(-4) M did not have any effect. These results demonstrate that cisplatin stimulates transcription from the c-myc promoter in erythroleukemia cells and may support the use of carboplatin as compared to cisplatin in the treatment of cancer patients.
Eliopoulos
A
A
Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece.
Kerr
D J
DJ
Spandidos
D A
DA
D000818:Animals; D016190:Carboplatin / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D002945:Cisplatin / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D016259:Genes, myc / Q000187:drug effects* / Q000502:physiology; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D051379:Mice; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000187:drug effects* / Q000502:physiology; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured
D016190:Carboplatin; D015500:Chloramphenicol O-Acetyltransferase; D002945:Cisplatin