Effect of castration and anatomical site on the plasma membrane structure and the activities of adenylate cyclase and 5'-nucleotidase in pig adipocytes.
The effects of castration on fat cell plasma membrane structure and enzyme activities (adenylate cyclase and 5'-nucleotidase) were studied in pig adipose tissues in two fat deposits (subcutaneous and perirenal). Castration induced a fat cell enlargement in both tissues. Membrane cholesterol content was reduced and fluidity was increased in perirenal fat from castrated animals. Castration had no effect on 5'-nucleotidase activity which was higher in subcutaneous than in perirenal in both kinds of animals. Adenylate cyclase activity was studied in the presence of different effectors: isoproterenol-stimulations of the enzyme were not affected by castration but were site-specific. GppNHp-stimulated activities were increased in subcutaneous fat from castrated animals. Castration had no influence on forskolin stimulations. The magnitude of GppNHp- and forskolin-stimulated activities were found to be tissue-dependent. Membrane results are discussed in relation with castration-induced fat cell enlargement.
Nicolas
C
C
Laboratoire de Nutrition et Sécurité Alimentaire, INRA-CRJ, Jouy-en-Josas, France.
Demarne
Y
Y
Lecourtier
M J
MJ
Lhuillery
C
C
D015720:5'-Nucleotidase / Q000032:analysis / Q000378:metabolism*; D000262:Adenylyl Cyclases / Q000032:analysis / Q000378:metabolism*; D000273:Adipose Tissue / Q000166:cytology* / Q000201:enzymology* / Q000648:ultrastructure; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure; D002784:Cholesterol / Q000032:analysis; D005576:Colforsin / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008560:Membrane Fluidity / Q000502:physiology; D008563:Membrane Lipids / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D009919:Orchiectomy; D010743:Phospholipids / Q000032:analysis; D013552:Swine
D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids; D005576:Colforsin; D002784:Cholesterol; D015720:5'-Nucleotidase; D000262:Adenylyl Cyclases; D007545:Isoproterenol