Time-dependent changes of markers associated with inflammation in the lungs of humans exposed to ambient levels of ozone.
Acute exposure of humans to 0.4 ppm ozone results in reversible respiratory function decrements, and cellular and biochemical changes leading to the production of substances which can mediate inflammation and acute lung injury. While pulmonary function decrements occur almost immediately after ozone exposure, it is not known how quickly the cellular and biochemical changes indicative of inflammation occur in humans. Changes in neutrophils and PGE2 have been observed in humans as early as 3 hr (28) and as late as 18 hr post exposure (19). The purpose of this study was to determine whether inflammatory changes occur relatively rapidly (within 1 hr) following exposure to ozone, or if the cascade of events which are initiated by ozone and lead to inflammation, take some time to develop. We exposed 10 healthy volunteers twice: once to filtered air and once to 0.4 ppm ozone. Each exposure lasted for 2 hr at an exercise level of 60 L/min, and bronchoalveolar lavage was performed 1 hr following exposure. The data from this study were compared to those from a previous study in which 10 subjects were exposed to O3 under identical conditions except that bronchoalveolar lavage was performed 18 hr following exposure. The results of the present study demonstrate that O3 is capable of inducing rapid cellular and biochemical changes in the lung. These changes were detectable as early as 1 hr following a 2 hr exposure of humans to ozone. The profiles of these changes were different at 1 hr and 18 hr following ozone exposures.(ABSTRACT TRUNCATED AT 250 WORDS)
Koren
H S
HS
Health Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina 27711.
Devlin
R B
RB
Becker
S
S
Perez
R
R
McDonnell
W F
WF
D000293:Adolescent; D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D015232:Dinoprostone / Q000032:analysis; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis; D008297:Male; D009504:Neutrophils / Q000187:drug effects; D010126:Ozone / Q000633:toxicity*; D011014:Pneumonia / Q000139:chemically induced*; D013925:Thromboplastin / Q000032:analysis; D013997:Time Factors
D015850:Interleukin-6; D010126:Ozone; D013925:Thromboplastin; D015232:Dinoprostone