title: Selective medium containing fosfomycin, nalidixic acid, and culture supernatant of Rhodococcus equi for isolation of Corynebacterium pseudotuberculosis.
abstract: FNR medium containing fosfomycin, nalidixic acid, bovine blood and culture supernatant of Rhodococcus equi was prepared by the present authors, and the medium did not inhibit growth of Corynebacterium pseudotuberculosis but completely hampered the growth of Bacillus subtilis, Staphylococcus aureus, and Escherichia coli. The culture supernatant of R. equi facilitated detection of suspected colonies of C. pseudotuberculosis due to synergistic hemolysis. Rate of isolation of the organisms (from the trachea, larynx and nasal cavity of 16 slaughtered sheep with caseous abscess in the lung) was higher with FNR, the selective medium, than with nonselective medium. The selective medium was thus found to be useful for isolation of C. pseudotuberculosis from sheep.
authors.affiliation: School of Veterinary Medicine, Rakuno Gakuen University.
authors.forename: H K
authors.initials: HK
authors.lastname: Zhao
authors.forename: T
authors.initials: T
authors.lastname: Hiramune
authors.forename: N
authors.initials: N
authors.lastname: Kikuchi
authors.forename: R
authors.initials: R
authors.lastname: Yanagawa
authors.forename: S
authors.initials: S
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Hatta
authors.forename: S
authors.initials: S
authors.lastname: Serikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Oe
mesh_terms: D000818:Animals; D016925:Corynebacterium pseudotuberculosis / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D005578:Fosfomycin / Q000494:pharmacology*; D006461:Hemolysis; D009268:Nalidixic Acid / Q000494:pharmacology*; D016929:Rhodococcus equi / Q000254:growth & development*; D012756:Sheep
chemical_list: D003470:Culture Media; D005578:Fosfomycin; D009268:Nalidixic Acid