Separation of some platinum(II) complexes by ionic strength gradient on a solvent-generated ion-exchange sorbent.
The compatibility of ionic strength gradient with solvent-generated ion-exchange chromatography on an octadecylsilica sorbent was proven for a mobile phase containing octanesulphonate. Only a slight baseline shift was observed during the gradient of the phosphate buffer, even at 210 nm. An equilibration time of 3 min between the runs was sufficient to obtain retention times with a reproducibility better than 1%. The compounds separated were cisplatin, carboplatin and related neutral and cationic platinum(II) complexes, including transplatin and the aquation products of cisplatin.
Macka
M
M
Lachema, Research Institute of Fine Chemicals, Brno, Czechoslovakia.
Borák
J
J
Kiss
F
F
D016190:Carboplatin / Q000032:analysis; D002852:Chromatography, Ion Exchange / Q000379:methods*; D002945:Cisplatin / Q000032:analysis; D009944:Organoplatinum Compounds / Q000032:analysis*; D009994:Osmolar Concentration; D015203:Reproducibility of Results
D009944:Organoplatinum Compounds; C034697:transplatin; D016190:Carboplatin; D002945:Cisplatin