Glycation of lysozyme in a restricted water environment.
Fast atom bombardment mass spectrometry (FAB) was used to determine the glycation sites of lysozyme in a restricted water environment. A 30-day incubation at 25 degrees C, and 65% relative humidity (R.H.) resulted in glycation at lysine-1 while a much shorter (3-day) incubation at 50 degrees C and 65% R.H. resulted in diglycation at lysine-1 as well as glycation at lysine-13 and lysine-33.
Wu
H
H
Department of Food Science, Cook College, Rutgers, The State University of New Jersey.
Hartman
T G
TG
Govindarajan
S
S
Kahn
P C
PC
Ho
C T
CT
Rosen
J D
JD
D000595:Amino Acid Sequence; D002918:Chymotrypsin / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006031:Glycosylation; D006813:Humidity; D008969:Molecular Sequence Data; D009113:Muramidase / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D012984:Software; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013997:Time Factors
D010446:Peptide Fragments; D009113:Muramidase; D002918:Chymotrypsin; D005947:Glucose