Identification of (23S)-5 alpha-cholestane-3 alpha,7 alpha,12 alpha,23,25-pentol in urine of patients with cerebrotendinous xanthomatosis.
This paper describes the identification of a new bile alcohol possessing the 5 alpha-cholestane structure that was found in the urine of patients with cerebrotendinous xanthomatosis. The urine samples were extracted with reversed-phase resin, treated with beta-glucuronidase, and separated on silica gel and reversed-phase column chromatography. The new bile alcohol isolated was the second component of the urinary bile alcohols and was identified as (23S)-5 alpha-cholestane-3 alpha,7 alpha,12 alpha,23,25-pentol by means of gas-liquid chromatography/mass spectrometry and nuclear magnetic resonance spectroscopic studies.
Kihira
K
K
Institute of Pharmaceutical Science, Hiroshima University School of Medicine, Japan.
Fukuda
K
K
Kuramoto
T
T
Kuriyama
M
M
Fujiyama
J
J
Osame
M
M
Hoshita
T
T
D001927:Brain Diseases / Q000652:urine*; D002777:Cholestanols / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013710:Tendons; D014973:Xanthomatosis / Q000652:urine*
D002777:Cholestanols; C019302:cholestane-3,7,12,23,25-pentol