Endotoxin stimulates endothelin release from cultured epithelial cells of guinea-pig trachea.
We examined the release of endothelin from cultured epithelial cells of guinea-pig trachea in response to treatment with endotoxin, using a sandwich-enzyme immunoassay. Cultured epithelial cells released endothelin in a time-dependent fashion (3, 6, 12, 24 h), and endotoxin (4-40 micrograms/ml) significantly increased endothelin release. Endotoxin (4-10 micrograms/ml) showed no cytotoxicity against epithelial cells. These results suggest that guinea-pig airway epithelial cells are capable of producing endothelin and this peptide may be related to the pathophysiological effects of endotoxin.
Ninomiya
H
H
Division of Pulmonary Medicine, University of Tsukuba, Ibaraki, Japan.
Uchida
Y
Y
Ishii
Y
Y
Nomura
A
A
Kameyama
M
M
Saotome
M
M
Endo
T
T
Hasegawa
S
S
D000818:Animals; D002478:Cells, Cultured; D016232:Endothelins / Q000096:biosynthesis / Q000378:metabolism*; D004731:Endotoxins / Q000494:pharmacology*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D006168:Guinea Pigs; D013268:Stimulation, Chemical; D014132:Trachea / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D016232:Endothelins; D004731:Endotoxins