Application of a metabolic balancing technique to the analysis of microbial fermentation data.
A general method for the development of fermentation models, based on elemental and metabolic balances, is illustrated with three examples from the literature. Physiological parameters such as the (maximal) yield on ATP, the energetic maintenance coefficient, the P/O ratio and others are estimated by fitting model equations to experimental data. Further, phenomenological relations concerning kinetics of product formation and limiting enzyme activities are assessed. The results are compared with the conclusions of the original articles, and differences due to the application of improved models are discussed.
de Hollander
J A
JA
Gist-Brocades, Delft, The Netherlands.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001216:Asparagine / Q000378:metabolism; D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism; D002175:Candida / Q000254:growth & development* / Q000378:metabolism; D002951:Citrates / Q000096:biosynthesis; D019343:Citric Acid; D003013:Clostridium / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D008353:Mannitol / Q000378:metabolism; D008433:Mathematics; D008954:Models, Biological; D010101:Oxygen Consumption; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D012231:Rhizobium / Q000378:metabolism
D002951:Citrates; D011135:Polysaccharides, Bacterial; D019343:Citric Acid; D008353:Mannitol; D001216:Asparagine; D000255:Adenosine Triphosphate; D005947:Glucose