Stability of sonicated aqueous suspensions of phospholipids under air.
The stability of phospholipids in liposomal aqueous suspension against oxidative degradation in air was investigated using spectrophotometric indices, glutathione peroxidase reactivity and thin layer chromatography. Zwitterionic phospholipid was found to be susceptible to degradation via oxidation of polyunsaturated hydrocarbon chains and ester hydrolysis, producing oxidized lysophosphatide and free fatty acid derivatives. These products were characterized as hydroperoxides based on their reactivity with the selenium-dependent glutathione peroxidase isolated from human erythrocytes. Lecithin in Tris buffer was more resistant to hydrolysis than in water. The sonication of 8.0 mM of soybean phosphatidylcholine (SB-PC) suspension in 0.1 M Tris (pH 7.5) in the presence of air produced relatively high concentration of conjugated diene hydroperoxide, but a small amount of hydrolyzed products. Anionic phospholipids, such as egg-phosphatidylglycerol (egg-PG), demonstrated higher resistance to air oxidation than the zwitterionic lecithin, but its oxidation was promoted by sonication.
Almog
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New York State Department of Health, Wadsworth Center for Laboratories and Research, Albany 12201-0509.
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Samsonoff
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D000388:Air; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D004355:Drug Stability; D004912:Erythrocytes / Q000201:enzymology*; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood*; D006801:Humans; D006868:Hydrolysis; D008081:Liposomes / Q000737:chemistry*; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000737:chemistry; D013010:Sonication; D014867:Water
D008081:Liposomes; D010713:Phosphatidylcholines; D014867:Water; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase