Differences in the probability of cloning specific DNA between primary and amplified libraries: theoretical considerations.
We present a theoretical study of the probability of isolating a particular clone from a DNA library. There are differences in this probability between primary and amplified libraries even if the desired clone represents the same fraction of both libraries. As the result, we must screen severalfold more phage or bacteria in an amplified library than in a primary library.
Kubo
A
A
Environmental Biology Division, National Institute for Environmental Studies, Ibaraki, Japan.
Kondo
N
N
D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D005784:Gene Amplification; D015698:Genomic Library; D011336:Probability
D004247:DNA