title: Rapid high-performance liquid chromatographic method for the determination of propranolol levels in canine and feline plasma.
abstract: A sensitive high-performance liquid chromatographic method that does not require organic extraction has been developed for the determination of propranolol levels in canine and feline plasma. Equal volumes of plasma and a mixture of methanol-acetonitrile-0.1 M sodium hydroxide (3:3:4, v/v/v) were added to a microseparation unit with a 10,000 molecular mass cut-off filter. The ultrafiltrate was analyzed by reversed-phase liquid chromatography with fluorimetric detection. The consistency of the recoveries obtained eliminated the need for an internal standard (coefficients of variation less than 4%). Linear regressions for the standard curves (2.5-100 ng/ml) gave correlation coefficients above 0.9955. The detection limit was 1 ng/ml. The assay retains high sensitivity while eliminating laborious sample preparation.
authors.affiliation: Division of Pharmacology, Wellcome Research Laboratories, Research Triangle Park, NC 27709.
authors.forename: K M
authors.initials: KM
authors.lastname: Hedeen
authors.forename: K
authors.initials: K
authors.lastname: Tyczkowska
authors.forename: D P
authors.initials: DP
authors.lastname: Aucoin
authors.forename: R M
authors.initials: RM
authors.lastname: Norton
mesh_terms: D000818:Animals; D002415:Cats; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D004285:Dogs; D008297:Male; D011433:Propranolol / Q000097:blood*; D013050:Spectrometry, Fluorescence / Q000662:veterinary
chemical_list: D011433:Propranolol