A follicular fluid factor inhibiting Xenopus oocyte maturation.
A factor was isolated from bovine follicular fluid (FF) that blocked progesterone-induced maturation of Xenopus oocytes. The factor was purified by ultrafiltration through PM-10 membrane, gel filtration on Sephadex G-25 column, gel permeation chromatography on Superose 12 column and reversed-phase HPLC on Partisil ODS-3 column. The active factor is a peptide with an estimated Mr of 8000 and it effectively blocked progesterone-induced maturation of Xenopus oocytes at a concentration of 100 micrograms/ml. On the other hand, it did not influence the spontaneous maturation of isolated cumulus-enclosed or denuded mouse oocytes. The present findings suggest that the FF factor may be an inhibitor or antagonist of progesterone action.
Kadam
A L
AL
Center for Biomedical Research, Population Council, New York, NY 10021.
Koide
S S
SS
D000818:Animals; D001685:Biological Factors / Q000302:isolation & purification / Q000494:pharmacology*; D016922:Cellular Senescence / Q000187:drug effects; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry*; D051379:Mice; D009865:Oocytes / Q000187:drug effects*; D011374:Progesterone / Q000037:antagonists & inhibitors*; D014462:Ultrafiltration; D014981:Xenopus
D001685:Biological Factors; D011374:Progesterone