title: [A method of determining the levels of deoxynivalenol (vomitoxin) and its metabolites].
abstract: A method has been developed for assay of deoxynivalenol (DON) and its metabolite desepoxideoxynivalenol (DOM-1) in animal excrements with the use of reversed-phase high-performance liquid chromatography with UV-detection (218 nm). The method includes purification in the mini-column with activated carbon and aluminium oxide. The detection limit was 50 ng/g, relative standard deviation--0.05-0.1, the degree of toxin isolation--76-89%. DON isolated from Fusarium macroceras, strain 579a, cultivated in rice under laboratory conditions, and DOM-1 obtained as a result of DON incubation with the contents of the beef first stomach, were used in the study. The structure of toxins isolated has been proved by the mass-spectrometry method. The method developed by the authors was used in the study of DON metabolism in vivo in monkeys.
authors.forename: V A
authors.initials: VA
authors.lastname: Tutel'ian
authors.forename: V S
authors.initials: VS
authors.lastname: Sobolev
authors.forename: I R
authors.initials: IR
authors.lastname: Katrush
authors.forename: F A
authors.initials: FA
authors.lastname: Medvedev
authors.forename: L P
authors.initials: LP
authors.lastname: Zakharova
mesh_terms: D000818:Animals; D002521:Cercopithecus; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005243:Feces / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D051381:Rats; D014255:Trichothecenes / Q000302:isolation & purification* / Q000652:urine
chemical_list: D014255:Trichothecenes; C007262:deoxynivalenol
vernacular_title: Metod opredeleniia dezoksinivalenola (vomitoksina) i ego metabolitov.