Conformation and quantification of chloramphenicol in cow's urine, muscle and eggs by electron capture negative ion chemical ionization gas chromatography/mass spectrometry.
A method is described for the determination of residues of the antibiotic chloramphenicol in biological samples. The method is based on gas chromatography/negative ion chemical ionization mass spectrometry and uses (37Cl2)chloramphenicol as internal standard. Selective ion monitoring of four analyte-specific ions enables the determination of chloramphenicol levels in urine of 3 micrograms l-1 with a coefficient of variation of 8%. The limit of detection of the method is 0.1 p.p.b. for urine, muscle and egg.
van der Heeft
E
E
National Institute of Public Health and Environmental Protection, Laboratory of Organic-Analytical Chemistry, Bilthoven, The Netherlands.
de Jong
A P
AP
van Ginkel
L A
LA
van Rossum
H J
HJ
Zomer
G
G
D000818:Animals; D002417:Cattle; D002701:Chloramphenicol / Q000032:analysis* / Q000652:urine; D004350:Drug Residues / Q000032:analysis*; D004531:Eggs / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D009132:Muscles / Q000737:chemistry*
D002701:Chloramphenicol