Influence of water miscible organic solvents on orange flavedo peptide amidase.
Due to the broad substrate spectrum and stability in some water-miscible organic solvents, the peptide amidase from orange flavedo was found to be a suitable enzyme for experiments in water-miscible organic solvents. Immobilization and the kind of organic solvent had no significant influence on the kinetic data while the polarity of the substrate caused a great difference in the apparent Km values. The results are explained by inadequate partition of the substrate between the microenvironment of the enzyme and the reaction solution.
Steinke
D
D
Institut für Enzymtechnologie, Heinrich-Heine Universität Düsseldorf, Jülich, FRG.
Kula
M R
MR
D000581:Amidohydrolases / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D007700:Kinetics; D010455:Peptides / Q000737:chemistry; D010944:Plants / Q000201:enzymology; D012997:Solvents; D013379:Substrate Specificity; D014867:Water
D004800:Enzymes, Immobilized; D010455:Peptides; D012997:Solvents; D014867:Water; D000581:Amidohydrolases