A simple technique for large scale in vitro culture of Plasmodium falciparum.
The large scale in vitro cultivation method of Fairlamb et al (1985) was modified to contain human plasma-supplemented medium in HEPES buffer. After 4 days with no change of medium nor agitation of the culture flask, 27- to 50-fold increase in the starting parasitemia of trophozoites were obtained.
Chavalitshewinkoon
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P
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
Wilairat
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P
D000818:Animals; D002021:Buffers; D003470:Culture Media; D006801:Humans; D010950:Plasma Cells; D010963:Plasmodium falciparum; D014776:Virus Cultivation / Q000379:methods
D002021:Buffers; D003470:Culture Media