Daflon as a cellular antioxidant and a membrane-stabilizing agent in human fibroblasts irradiated by ultraviolet A radiation.
Daflon is a strong inhibitor of Cu(2+)-induced arachidonic acid peroxidation, as revealed by the inhibition of thiobarbituric acid-reactive substance formation in mixed liposomes of phosphatidylcholine and arachidonic acid. Diosmin, the major Daflon constituent, is a good complexant of Cu2+ ions but not of Fe2+ ions. The Cu2+ complex formation may thus explain part of the antioxidant effect. However, Daflon is also a good quencher of the singlet oxygen-induced arachidonic acid peroxidation that does not involve metal ions. When cultured human fibroblasts are incubated with the diosmin-hesperidin mixture constituting Daflon, the latter is as efficient as vitamin E in inhibiting the ultraviolet A-induced lipid peroxidation and plasma membrane damage measured by lactate dehydrogenase release.
Santus
R
R
Muséum National d'Histoire Naturelle, Laboratoire de Physico-Chimie de l'Adaptation Biologique, Paris, France.
Perdrix
L
L
Haigle
J
J
Morliere
P
P
Maziere
J C
JC
Maziere
C
C
Labrid
C
C
D000975:Antioxidants / Q000494:pharmacology; D001095:Arachidonic Acids / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured; D003300:Copper / Q000378:metabolism; D004145:Diosmin / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D006569:Hesperidin / Q000494:pharmacology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation; D014466:Ultraviolet Rays / Q000009:adverse effects; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D001095:Arachidonic Acids; D014810:Vitamin E; D003300:Copper; D004145:Diosmin; D006569:Hesperidin; D007770:L-Lactate Dehydrogenase