title: High-performance liquid chromatographic assay of 5-aminosalicylic acid (5-ASA) and its metabolites N-beta-D-glucopyranosyl-5-ASA, N-acetyl-5-ASA, N-formyl-5-ASA and N-butyryl-5-ASA in biological fluids.
abstract: A fast, highly sensitive high-performance liquid chromatographic method for the simultaneous determination of 5-aminosalicylic acid (5-ASA) and its metabolites N-beta-D-glucopyranosyl-5-ASA, N-formyl-5-ASA, N-acetyl-5-ASA and N-butyryl-5-ASA has been developed using a dynamically modified silica approach on a 40 mm x 4.6 mm I.D. column packed with 3-microns Hypersil. Plasma proteins are precipitated with acetonitrile. After extraction of the acetonitrile into 1,1,1-trichloroethane an undiluted aqueous phase containing the analytes is obtained. The detection limits are in the range 0.002-0.05 microgram/ml in plasma at a signal-to-noise ratio of 3 using fluorescence detection.
authors.affiliation: Department of Organic Chemistry, Royal Danish School of Pharmacy, Copenhagen, Denmark.
authors.forename: J
authors.initials: J
authors.lastname: Tjørnelund
authors.forename: S H
authors.initials: SH
authors.lastname: Hansen
mesh_terms: D000636:Aminosalicylic Acids / Q000097:blood / Q000378:metabolism* / Q000652:urine; D001826:Body Fluids / Q000737:chemistry*; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012997:Solvents; D013050:Spectrometry, Fluorescence
chemical_list: D000636:Aminosalicylic Acids; D002021:Buffers; D012997:Solvents