Antibodies against Entamoeba histolytica in individuals with intestinal amoebiasis presenting cysts and/or trophozoites in the feces.
Serum samples were obtained from 154 individuals infected with Entamoeba histolytica (78 symptomatic and 76 asymptomatic). Twelve had trophozoites in the feces whereas 142 had only cysts. The sera were used to test the existence of antibodies anti-Entamoeba histolytica employing the Indirect Hemagglutination (IHA), Indirect Immunofluorescence (IFAT), Complement Fixation Reaction (CFR) and Counterimmunoelectrophoresis (CIEP). For those individuals with trophozoites in their feces, 75.0 were positive by IHA and IFAT, 83.0 by CFR and 41.7 by CIEP. In individuals who had only cysts, positive results by the same tests were respectively, 5.6%, 12.0%, 19.0% and 5.6%. The difference in relation to the titers of antibodies detected through IHA, IFAT, CFR and CIEP and in relation to the presence of trophozoites or cysts in the feces was significative for four immunological reactions when chi 2, was employed (P < 0.05).
Carvalho M das
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Department of Clinical and Toxicologycal Analyses, Pharmacy Faculty, Federal Minas Gerais University, Brazil.
Guimaräes
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Silva
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EF
D000818:Animals; D000913:Antibodies, Protozoan / Q000302:isolation & purification*; D001938:Brazil; D004404:Dysentery, Amebic / Q000175:diagnosis / Q000276:immunology*; D004748:Entamoeba histolytica / Q000276:immunology*; D005243:Feces / Q000469:parasitology*; D006801:Humans
D000913:Antibodies, Protozoan