Production and characterization of a monoclonal antibody to the v-mos oncogene protein.
Valuable information about proto-oncogenes and their physiological functions has been obtained by studying their expression in normal cells. However, the protein product of the c-mos gene, the cellular homologue of the transforming gene (v-mos) of Moloney murine sarcoma virus, has not been detected in normal mouse cells or tissues. Here, we have constructed a v-mos expression vector, pRI-delta mos, which directs the synthesis of a truncated v-mos gene product, a protein A fusion protein. Using the truncated v-mos oncoprotein produced in Escherichia coli as immunogen, we prepared anti-v-mos monoclonal antibodies (MAbs). In immunoblotting assays, the MAb was reactive with v-mos oncoprotein and detected bands at 43 KDa or 39 kDa in the tissue extract of mouse testes or ovaries, respectively, in which the c-mos protooncogene mRNA is expressed. These results demonstrate that the v-mos MAb obtained is suitable for elucidating the physiological functions of v-mos gene product and may also be utilized to detect c-mos gene product at the cellular level.
Ishikawa
K
K
Division of Cell Biology, Fujita Health University, Aichi, Japan.
Kurata
N
N
Aoki
H
H
Kato
T
T
Marunouchi
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000378:metabolism; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D006825:Hybridomas / Q000276:immunology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D008980:Moloney murine sarcoma virus / Q000235:genetics / Q000276:immunology; D017232:Oncogene Proteins v-mos; D010053:Ovary / Q000276:immunology; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000276:immunology; D017231:Proto-Oncogene Proteins c-mos; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics / Q000276:immunology*; D013737:Testis / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral; D017232:Oncogene Proteins v-mos; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D015682:Retroviridae Proteins, Oncogenic; D017231:Proto-Oncogene Proteins c-mos