Simian virus 40 large-T antigen expresses a biological activity complementary to the p300-associated transforming function of the adenovirus E1A gene products.
In this report we present evidence that simian virus 40 T antigen encodes a biological activity that is functionally equivalent to the transforming activity lost by deletion of the E1A p300-binding region. T-antigen constructs from which the pRb-binding region has been deleted are virtually unable to induce foci of transformed cells in a ras cooperation assay in primary baby rat kidney cells. Nevertheless, such a construct can cooperate with an E1A N-terminal deletion mutant, itself devoid of transforming activity, to induce foci in this assay. The heterologous trans-cooperating activity observed between E1A and T-antigen deletion products is as efficient as trans cooperation between mutants expressing individual E1A domains. The cooperating function can be impaired by a deletion near the N terminus of T antigen. Such a deletion impairs neither the p53-binding function nor the activity of the pRb-binding region.
Yaciuk
P
P
Cold Spring Harbor Laboratory, New York 11724.
Carter
M C
MC
Pipas
J M
JM
Moran
E
E
D017456:Adenovirus Early Proteins; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D015870:Gene Expression; D005816:Genetic Complementation Test; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D011233:Precipitin Tests; D051381:Rats; D013539:Simian virus 40 / Q000276:immunology*
D017456:Adenovirus Early Proteins; D000952:Antigens, Polyomavirus Transforming; D009856:Oncogene Proteins, Viral