In-vivo antithrombotic potency of placenta protein 4 (annexin V).
The antithrombotic properties of Placenta Protein 4 (PP4) were investigated in laser or photochemically induced thrombus formation models in rats. In both in-vivo test-systems PP4 displayed a significant antithrombotic effect at dose levels as low as 0.3 and 1.0 mg/kg body weight. Bleeding times, surprisingly, were not prolonged significantly at these dose regimens. Maximal inhibition of thrombus formation in the laser-model was observed 15 min after intravenous administration of PP4, but was not recognizable in a clear-cut reaction in the second model. Determination of PP4 plasma levels in two monkeys revealed a half-life of 11.5 and 14.9 min, respectively. The maximal anticoagulant effect was observed between 15 and 30 min after administration of PP4 as determined functionally by means of thrombelastography.
Römisch
J
J
Research Laboratories of Behringwerke AG, Marburg, FRG.
Seiffge
D
D
Reiner
G
G
Pâques
E P
EP
Heimburger
N
N
D000818:Animals; D017304:Annexin A5; D001760:Bleeding Time; D002135:Calcium-Binding Proteins / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology*; D005343:Fibrinolytic Agents; D006207:Half-Life; D007834:Lasers; D008252:Macaca fascicularis; D008297:Male; D010777:Photochemistry; D011257:Pregnancy Proteins / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology*; D051381:Rats; D013916:Thrombelastography; D013927:Thrombosis / Q000097:blood / Q000209:etiology / Q000517:prevention & control*
D017304:Annexin A5; D002135:Calcium-Binding Proteins; D005343:Fibrinolytic Agents; D011257:Pregnancy Proteins