Differential expression of I alpha- and II alpha-globin genes in Norwegian dairy goats.
Fast protein liquid chromatography (FPLC) and polyacrylamide gel electrophoresis were used to determine the ratio between I alpha- and II alpha-globin chains in Norwegian dairy goats. Three different phenotypes, designated normal (N) with I alpha- to II alpha-globin ratio 3:1, reversed (R) with ratio 1:2 and double-reversed (RR) with no I alpha-globin, were described. Family studies indicated that the R animals were heterozygous, and the RR animal homozygous, for a haplotype without a functional I alpha-globin product. Southern blot analysis of goat DNAs digested with six different restriction enzymes showed that the different ratios of alpha chain expression could not be due to a deletion of the I alpha-gene and/or duplication or triplication of the II alpha-globin genes. The homozygous reversed animal with no detectable I alpha-globin had a mild anaemia.
Bergersen
O
O
Department of Animal Genetics, Norwegian College of Veterinary Medicine/National Veterinary Institute, Oslo.
Fosse
V M
VM
Nesse
L L
LL
D000740:Anemia / Q000235:genetics / Q000662:veterinary; D000818:Animals; D015139:Blotting, Southern; D002851:Chromatography, High Pressure Liquid; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015870:Gene Expression / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D005914:Globins / Q000032:analysis / Q000235:genetics*; D006041:Goats / Q000235:genetics*; D006454:Hemoglobins / Q000032:analysis; D008297:Male; D009664:Norway; D010375:Pedigree; D010641:Phenotype
D015342:DNA Probes; D006454:Hemoglobins; D005914:Globins; D004262:DNA Restriction Enzymes