title: U1 snRNP can influence 3'-splice site selection as well as 5'-splice site selection.
abstract: To address the mechanisms that underlie splice site selection and splice site partner assignment, we analyzed the splicing of yeast (Saccharomyces cerevisiae) transcripts containing splice site region duplications. When the 5'-splice site region was duplicated, both sites were utilized to the same extent, indicating little or no influence of proximity on 5'-splice site choice. However, the effect of a 5'-mutant site was greatly enhanced by the presence of an adjacent wild-type site, and this effect was reversed by the restoration of base-pairing with U1 snRNA. 3'-Splice site choice was apparently influenced by proximity, as the site closest to the 5'-splice site was greatly preferred. Studies with strains carrying some U1 snRNA mutations showed an increase in the use of the distal 3'-splice site, indicating a role for U1 snRNP in 3'-splice site selection. The data are compared with those from mammalian splice site choice experiments and suggest mechanisms that influence differential splice site choice as well as exon skipping.
authors.affiliation: Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, Massachusetts 02254.
authors.forename: V
authors.initials: V
authors.lastname: Goguel
authors.forename: X L
authors.initials: XL
authors.lastname: Liao
authors.forename: B C
authors.initials: BC
authors.lastname: Rymond
authors.forename: M
authors.initials: M
authors.lastname: Rosbash
mesh_terms: D001483:Base Sequence; D002151:Calorimetry; D002872:Chromosome Deletion; D005800:Genes, Fungal; D007438:Introns; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D010957:Plasmids; D012326:RNA Splicing; D012342:RNA, Small Nuclear / Q000235:genetics*; D012261:Ribonucleoproteins / Q000235:genetics*; D017411:Ribonucleoproteins, Small Nuclear; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D012342:RNA, Small Nuclear; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D001616:beta-Galactosidase