Cycloheximide inhibits interferon-gamma-induced class II major histocompatibility complex antigen expression in cultured rat thyroid cells.
The effects of the agents that are related to intracellular events on interferon-gamma-induced class II major histocompatibility complex antigen expression were studied using the technique of immunocytochemistry. Rat class II major histocompatibility complex antigen (RT1.B) was expressed in 88.3 +/- 3.3% (n = 3) of the functioning rat thyroid cells (FRTL-5) cultured in a medium containing 100 U/ml recombinant rat interferon-gamma (IFN gamma). Deprivation of bovine TSH had no effect on the expression of RT1.B antigen by IFN gamma. A23187 (1 nM to 2 microM) and/or 10 nM to 10 microM phorbol 12-myristate 13-acetate did not induce the expression of RT1.B antigen. IFN gamma-induced RT1.B expression was not inhibited by either 10 nM to 100 microM 1-(5-isoquinolysulfonyl)-2-methylpiperazine or 200 nM to 200 microM 8-(N,N-dimethylamino)octyl-3,4,5-trimethoxybenzoate hydrochloride. It was also not inhibited by either 5-200 microM verapamil or 500 nM to 20 microM trifluoperazine. However, 0.01-10 micrograms/ml cycloheximide inhibited IFN gamma-induced RT1.B antigen expression in a dose-dependent manner. These results suggest that IFN gamma induces RT1.B antigen expression in FRTL-5 cells via de novo protein synthesis independent of the cAMP system, phosphatidylinositide system, and voltage-dependent calcium channel.
Lee
M S
MS
Department of Internal Medicine, Korea Cancer Center Hospital, Seoul.
Cho
B Y
BY
Kim
S Y
SY
Lee
H K
HK
Koh
C S
CS
Min
H K
HK
Lee
J O
JO
Kang
T W
TW
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002147:Calmodulin / Q000037:antagonists & inhibitors; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology*; D000949:Histocompatibility Antigens Class II / Q000037:antagonists & inhibitors*; D007371:Interferon-gamma / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000502:physiology; D011506:Proteins / Q000037:antagonists & inhibitors; D051381:Rats; D013961:Thyroid Gland / Q000166:cytology / Q000276:immunology*; D013972:Thyrotropin / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D002147:Calmodulin; D000949:Histocompatibility Antigens Class II; D010716:Phosphatidylinositols; D011506:Proteins; D003994:Bucladesine; D007371:Interferon-gamma; D013972:Thyrotropin; D003513:Cycloheximide; D014700:Verapamil