title: Several groups among human herpesvirus 6 strains can be distinguished by Southern blotting and polymerase chain reaction.
abstract: Eight human herpesvirus 6 (HHV-6) strains were studied by Southern blot and polymerase chain reaction. DNA from infected cells was digested by a panel of restriction enzymes and hybridized with cloned BamHI fragments corresponding to about 30% of the HHV-6 strain SIE genome. In parallel, this DNA was amplified by polymerase chain reaction using pairs of primers derived from the strain SIE nucleotide sequence. Subsequently, amplification products were analyzed by hybridization, digestion with restriction endonucleases, and partial nucleotide sequencing. Overall results indicated that all strains were closely related to one another. However, concordant differences in restriction patterns allowed at least two groups to be distinguished, typified by strains SIE and HST, respectively. Differences between the two groups were found to reflect a limited number of punctual changes in nucleotide sequences. These results strengthen the idea of a unique HHV-6 species with genetic polymorphism. In addition, this study provides useful markers for the diagnosis and molecular epidemiology of HHV-6 infections.
authors.affiliation: Laboratoire de Bactériologie-Virologie, C.E.R.V.I., Hôpital de la Pitié-Salpêtrière, Paris, France.
authors.forename: J T
authors.initials: JT
authors.lastname: Aubin
authors.forename: H
authors.initials: H
authors.lastname: Collandre
authors.forename: D
authors.initials: D
authors.lastname: Candotti
authors.forename: D
authors.initials: D
authors.lastname: Ingrand
authors.forename: C
authors.initials: C
authors.lastname: Rouzioux
authors.forename: M
authors.initials: M
authors.lastname: Burgard
authors.forename: S
authors.initials: S
authors.lastname: Richard
authors.forename: J M
authors.initials: JM
authors.lastname: Huraux
authors.forename: H
authors.initials: H
authors.lastname: Agut
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D004279:DNA, Viral / Q000235:genetics; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000382:microbiology; D015654:Herpesvirus 6, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015183:Restriction Mapping; D013045:Species Specificity
chemical_list: D004279:DNA, Viral