title: FVB/N: an inbred mouse strain preferable for transgenic analyses.
abstract: FVB/N mice offer a system suitable for most transgenic experiments and subsequent genetic analyses. The inbred FVB/N strain is characterized by vigorous reproductive performance and consistently large litters. Moreover, fertilized FVB/N eggs contain large and prominent pronuclei, which facilitate microinjection of DNA. The phenotype of large pronuclei in the zygote is a dominant trait associated with the FVB/N oocyte but not the FVB/N sperm. In experiments to generate transgenic mice, the same DNA constructs were injected into three different types of zygotes: FVB/N, C57BL/6J, and (C57BL/6J x SJL/J)F1. FVB/N zygotes survived well after injection, and transgenic animals were obtained with efficiencies similar to the F1 zygotes and much better than the C57BL/6J zygotes. Genetic markers of the FVB/N strain have been analyzed for 44 loci that cover 15 chromosomes and were compared with those of commonly used inbred strains. In addition to the albino FVB/N strain, pigmented congenic strains of FVB/N are being constructed. These features make the FVB/N strain advantageous to use for research with transgenic mice.
authors.affiliation: Jackson Laboratory, Bar Harbor, ME 04609.
authors.forename: M
authors.initials: M
authors.lastname: Taketo
authors.forename: A C
authors.initials: AC
authors.lastname: Schroeder
authors.forename: L E
authors.initials: LE
authors.lastname: Mobraaten
authors.forename: K B
authors.initials: KB
authors.lastname: Gunning
authors.forename: G
authors.initials: G
authors.lastname: Hanten
authors.forename: R R
authors.initials: RR
authors.lastname: Fox
authors.forename: T H
authors.initials: TH
authors.lastname: Roderick
authors.forename: C L
authors.initials: CL
authors.lastname: Stewart
authors.forename: F
authors.initials: F
authors.lastname: Lilly
authors.forename: C T
authors.initials: CT
authors.lastname: Hansen
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002678:Chimera; D005260:Female; D005298:Fertility; D005814:Genes, Viral; D005819:Genetic Markers; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D013539:Simian virus 40
chemical_list: D005819:Genetic Markers; D015345:Oligonucleotide Probes