Quantitation of biologically active IL-1 by a sensitive assay based on immobilized human IL-1 receptor type II (IL-1RII).
A rapid and sensitive solid-phase radioassay is described for the quantitative detection of human interleukin-1 (IL-1) based on its capability to bind the nitrocellulose-immobilized IL-1 receptor solubilized from plasma membranes of a subclone of the human B cell lymphoma Raji. The assay can detect human IL-1 beta levels as low as 1 X 10(-11) M, both in physiological buffers and in human plasma. Much lower sensitivity was observed for human IL-1 alpha (3.7 X 10(-9) M) and murine IL-1 beta (2 X 10(-9) M). This assay has the advantage to specifically detect only the correctly folded biologically active IL-1. Simple pretreatment procedure that selectively removes IL-1 beta from samples has been devised so that the ratio of the two IL-1s isoforms in the sample can be precisely determined. This assay represents a fast method for the simultaneous-testing of large numbers of biological samples.
Scapigliati
G
G
Sclavo Research Center, Siena, Italy.
Bossu
P
P
Censini
S
S
Tagliabue
A
A
Boraschi
D
D
Ghiara
P
P
D005374:Filtration; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis*; D007457:Iodine Radioisotopes; D011869:Radioligand Assay; D011971:Receptors, Immunologic / Q000378:metabolism*; D017472:Receptors, Interleukin-1
D007375:Interleukin-1; D007457:Iodine Radioisotopes; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1