A maize gene expressed during embryogenesis is abscisic acid-inducible and highly conserved.
We have isolated by differential hybridization a cDNA, termed Emb564, which is complementary to an 800 nt embryo-specific transcript in Zea mays. The expression of Emb564 can be enhanced by exogenous abscisic acid (ABA) and the effect of ABA on the accumulation of Emb564 transcript appears to be confined to embryos at the early and mid embryonic stages. In addition, Emb564 is expressed at low levels in ABA-deficient but not in ABA non-responsive embryos. Genomic analysis suggested that the Emb564 mRNA is encoded by a single gene. Sequence analysis showed that Emb564 exhibits extensive similarities with several known ABA-inducible genes.
Williams
B
B
Department of Biology, McGill University, Montreal, Quebec, Canada.
Tsang
A
A
D000040:Abscisic Acid / Q000502:physiology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis*; D003313:Zea mays / Q000196:embryology / Q000235:genetics*
D010940:Plant Proteins; D012333:RNA, Messenger; C070150:Emb564 protein, Zea mays; D000040:Abscisic Acid; D004247:DNA