title: Structure and evolution of a family of genes encoding antiseptic and disinfectant resistance in Staphylococcus aureus.
abstract: Resistance to antiseptics and disinfectants in Staphylococcus aureus, encoded by the qacC/qacD gene family, is associated with genetically dissimilar small, nontransmissible (pSK89) and large conjugative (pSK41) plasmids. The qacC and qacD genes were analysed in detail through deletion mapping and nucleotide sequence analysis, and shown to encode the same polypeptide, predicted to be 107 aa in size. Direct repeat elements flank the qacD gene, elements which also flank the qacC gene in truncated forms. These elements contain palA sequences, regions of DNA required for replication of some plasmids in S. aureus. The qacC gene is predicted to have evolved from the qacD gene, and in the process to have become reliant on new promoter sequences for its expression. The entire sequence of the 2.4-kb plasmid pSK89 (which contains qacC) was determined, and is compared with other plasmids from Gram + bacteria.
authors.affiliation: Department of Microbiology, Monash University, Clayton, Victoria, Australia.
authors.forename: T G
authors.initials: TG
authors.lastname: Littlejohn
authors.forename: D
authors.initials: D
authors.lastname: DiBerardino
authors.forename: L J
authors.initials: LJ
authors.lastname: Messerotti
authors.forename: S J
authors.initials: SJ
authors.lastname: Spiers
authors.forename: R A
authors.initials: RA
authors.lastname: Skurray
mesh_terms: D000595:Amino Acid Sequence; D000891:Anti-Infective Agents, Local; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid; D013211:Staphylococcus aureus / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D000891:Anti-Infective Agents, Local