Epstein Barr virus/complement C3d receptor is an interferon alpha receptor.
Interferon alpha contains a sequence motif similar to the complement receptor type two (CR2/CD21) binding site on complement fragment C3d. Antibodies against a peptide with the CR2 binding sequence on C3d react with a peptide carrying the IFN alpha CR2 binding motif (residues 92-99) and with recombinant IFN alpha. The IFN alpha-derived peptide, as well as recombinant IFN alpha, inhibits C3bi/C3d interaction with CR2 on the Burkitt lymphoma Raji. The direct interaction of IFN alpha and CR2 is inhibited by polyclonal anti-IFN alpha, anti-CR2 and anti-C3d peptide antibodies as well as by C3bi/C3d, EBV coat protein gp350/220 and IFN but not by IFN gamma. [125I]IFN alpha binding to Raji cells is inhibited by polyclonal anti-IFN alpha and anti-CR2 antibodies, by peptides with the CR2 binding motif and partially by C3bi/C3d. Monoclonal anti-CR2 antibody HB5, but not OKB-7, blocks IFN alpha binding to Raji cells. CR2 or CR2-like molecules may therefore be the major IFN alpha receptors on B lymphocytes.
Delcayre
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AX
California Institute for Biological Research, La Jolla 92037.
Salas
F
F
Mathur
S
S
Kovats
K
K
Lotz
M
M
Lernhardt
W
W
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D015933:Complement C3d / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism; D007739:L Cells / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008969:Molecular Sequence Data; D011951:Receptors, Complement / Q000235:genetics* / Q000378:metabolism; D017464:Receptors, Complement 3d; D011971:Receptors, Immunologic / Q000235:genetics* / Q000378:metabolism; D017471:Receptors, Interferon; D011991:Receptors, Virus / Q000235:genetics* / Q000502:physiology; D011994:Recombinant Proteins; D012397:Rosette Formation; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D007370:Interferon Type I; D011951:Receptors, Complement; D017464:Receptors, Complement 3d; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D011991:Receptors, Virus; D011994:Recombinant Proteins; D015933:Complement C3d