Proteinase inhibitors in Brazilian Leguminosae.
Serine proteinase inhibitors, in the seeds of several Leguminosae from the Pantanal region (West Brazil), were studied using bovine trypsin, a digestive enzyme, Factor XIIa and human plasma kallikrein, two blood clotting factors. The inhibitors were purified from Enterolobium contortisiliquum (M(r) = 23,000), Torresea cearensis (M(r) = 13,000), Bauhinia pentandra (M(r) = 20,000) and Bauhinia bauhinioides (M(r) = 20,000). E. contortisiliquum inhibitor inactivates all three enzymes, whereas the T. cearensis inhibitor inactivates trypsin and Factor XIIa, but does not affect plasma kallikrein; both Bauhinia inhibitors, on the other hand, inactivate trypsin and plasma kallikrein but only the B. pentandra inhibitor affects Factor XIIa. Ki values were calculated between 10(-7) and 10(-8) M.
Sampaio
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CA
Departamento de Bioquímica, Escola Paulista de Medicina, São Paulo, Brasil.
Oliva
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ML
Tanaka
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Sampaio
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MU
D001938:Brazil; D002846:Chromatography, Affinity; D007887:Fabaceae / Q000737:chemistry*; D015956:Factor XIIa / Q000037:antagonists & inhibitors; D007610:Kallikreins / Q000037:antagonists & inhibitors; D010946:Plants, Medicinal; D012639:Seeds; D015842:Serine Proteinase Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D014361:Trypsin Inhibitors / Q000302:isolation & purification / Q000494:pharmacology
D015842:Serine Proteinase Inhibitors; D014361:Trypsin Inhibitors; D007610:Kallikreins; D015956:Factor XIIa