title: In vitro membrane binding of the translation products of the carlavirus 7-kDa protein genes.
abstract: Two double-stranded DNA copies of the genes potentially coding for the 7-kDa proteins of potato virus M (PVM) and potato virus S (PVS) were synthesized and cloned into T7 transcription vectors. Cell-free translation of the corresponding monocistronic transcripts yielded in both cases a single protein of approximately 7-8 kDa that contains a highly hydrophobic N-terminal segment. To analyze their membrane-binding potential, both proteins were synthesized in the membrane-enriched Krebs-2 extract. It was found that the smooth membrane fraction was enriched in the carlavirus 7-kDa proteins. The primary and predicted secondary structures of their N-terminal hydrophobic segments suggest that the latter can function as signals for translocation into the rough endoplasmic reticulum.
authors.affiliation: Department of Virology, Moscow State University, USSR.
authors.forename: S Y
authors.initials: SY
authors.lastname: Morozov
authors.forename: N A
authors.initials: NA
authors.lastname: Miroshnichenko
authors.forename: A G
authors.initials: AG
authors.lastname: Solovyev
authors.forename: D A
authors.initials: DA
authors.lastname: Zelenina
authors.forename: O N
authors.initials: ON
authors.lastname: Fedorkin
authors.forename: L I
authors.initials: LI
authors.lastname: Lukasheva
authors.forename: S A
authors.initials: SA
authors.lastname: Grachev
authors.forename: B K
authors.initials: BK
authors.lastname: Chernov
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D011487:Protein Conformation; D012328:RNA Viruses / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D011198:Solanum tuberosum; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: C069857:7 kDa protein, carlavirus; D008565:Membrane Proteins; D014764:Viral Proteins