Reducing the concentration of selected marker improves efficiency of cotransfer of unselected DNA into SV40-transformed human fibroblasts.
A substantial increase in transfer of unselected DNA to two human SV40-transformed fibroblast cell lines was obtained by reducing the concentration of the cotransferred selected marker DNA. The average amount of unselected DNA transferred, even under favorable conditions, was still low compared to that reported for some rodent cell lines. Our results suggest that in human fibroblasts there is strong competition between exogenous DNA molecules for integration and maintenance, and that more unselected DNA is retained in the presence of only one copy of the selected marker.
Green
M H
MH
MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, United Kingdom.
Lowe
J E
JE
Teufel
R
R
Petit-Frère
C
C
D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004351:Drug Resistance / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology; D005819:Genetic Markers; D005821:Genetic Techniques; D006801:Humans; D010042:Ouabain / Q000494:pharmacology; D013539:Simian virus 40; D014162:Transfection
D005819:Genetic Markers; D010042:Ouabain