Expression of a maize sucrose phosphate synthase in tomato alters leaf carbohydrate partitioning.
We isolated a complementary DNA sequence for the enzyme sucrose phosphate synthase (SPS) from maize utilizing a limited amino acid sequence. The 3509-bp cDNA encodes a 1068-amino acid polypeptide. The identity of the cDNA was confirmed by the ability of the cloned sequence to direct sucrose phosphate synthesis in Escherichia coli. Because no plant-specific factors were necessary for enzymatic activity, we can conclude that SPS enzyme activity is conferred by a single gene product. Sequence comparisons showed that SPS is distantly related to the enzyme sucrose synthase. When expressed from a ribulose bisphosphate carboxylase small subunit promoter in transgenic tomatoes, total SPS activity was boosted up to sixfold in leaves and appeared to be physiologically uncoupled from the tomato regulation mechanism. The elevated SPS activity caused a reduction of starch and increase of sucrose in the tomato leaves. This result clearly demonstrates that SPS is involved in the regulation of carbon partitioning in the leaves.
Worrell
A C
AC
Calgene Inc., Davis, California 95616.
Bruneau
J M
JM
Summerfelt
K
K
Boersig
M
M
Voelker
T A
TA
D000595:Amino Acid Sequence; D001483:Base Sequence; D050260:Carbohydrate Metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D005964:Glucosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D010944:Plants / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D003313:Zea mays / Q000201:enzymology / Q000235:genetics
D004247:DNA; D005964:Glucosyltransferases; C022698:sucrose-phosphate synthase