Regulation of biosynthesis of L-amino acid oxidase by Neurospora crassa.
L-Amino acid oxidase was purified from liquid cultures of Neurospora crassa induced by L-phenylalanine, D-phenylalanine, ATP and cycloheximide. Although the four enzyme species isolated were found to differ in size and electrophoretic mobility, they were the product of a single gene as demonstrated by genomic Southern analysis. Northern analysis of total cellular RNA showed a rapid increase of L-amino acid oxidase mRNA in response to the different inducing agents studied. These data suggest that the L-amino acid oxidase gene from N. crassa is regulated at the transcriptional level.
Niedermann
D M
DM
Biochemisches Institut der Universität Zürich, Switzerland.
Lerch
K
K
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis*; D015152:Blotting, Northern; D015139:Blotting, Southern; D002455:Cell Division; D003513:Cycloheximide / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D015966:Gene Expression Regulation, Fungal; D007527:Isoenzymes; D050830:L-Amino Acid Oxidase; D009492:Neurospora crassa / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D010649:Phenylalanine / Q000494:pharmacology
D007527:Isoenzymes; D010649:Phenylalanine; D000255:Adenosine Triphosphate; D003513:Cycloheximide; D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase