title: In vitro action of some disinfectants on Paracoccidioides brasiliensis yeast forms.
abstract: The fungicidal action of sodium hypochlorite (0.3, 1, 2.5, 5 and 10%); formaldehyde (2, 5, and 10%); and ethyl alcohol (70%) on yeast forms of Paracoccidioides brasiliensis Pb 18 and a newly-isolated Goiana strain was described. Contact between the fungus and the disinfectants was maintained for 1, 2, 24, 48 and 72 hours at room temperature. Viability was evaluated by the fluorescein diacetate-ethidium bromide treatment, culture in solid and liquid media (36 degrees C and 26 degrees C); yeast to mycelial germination at room temperature; and radiometric study of metabolic activity. All concentrations of disinfectants were found to be effective in inactivating Pb 18 and Goiana strains, except for the 1-hour contact with 2% formaldehyde, in which fluorescein diacetate-ethidium bromide treatment was found to reveal 40 and 27% of viable cells, respectively. The yeast to mycelial germination method was considered to reveal faster and similar results as compared to culture in solid and liquid media.
authors.affiliation: Depto. de Doenças Infecciosas e Parasitárias da FM-USP, Hospital das Clínicas da FM-USP (LIM-48), São Paulo, Brasil.
authors.forename: M A
authors.initials: MA
authors.lastname: Shikanai-Yasuda
authors.forename: R T
authors.initials: RT
authors.lastname: Taguchi
authors.forename: M K
authors.initials: MK
authors.lastname: Sato
authors.forename: N T
authors.initials: NT
authors.lastname: Melo
authors.forename: C M
authors.initials: CM
authors.lastname: Assis
authors.forename: R C
authors.initials: RC
authors.lastname: Nigro
authors.forename: E E
authors.initials: EE
authors.lastname: Camargo
authors.forename: C S
authors.initials: CS
authors.lastname: Lacaz
authors.forename: V
authors.initials: V
authors.lastname: Amato Neto
authors.forename: A
authors.initials: A
authors.lastname: Sesso
mesh_terms: D000431:Ethanol / Q000494:pharmacology*; D005557:Formaldehyde / Q000494:pharmacology*; D008854:Microscopy, Electron; D010228:Paracoccidioides / Q000187:drug effects* / Q000648:ultrastructure; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D005557:Formaldehyde; D000431:Ethanol; D012973:Sodium Hypochlorite