Capture of flanking DNA by a P element in Drosophila melanogaster: creation of a transposable element.
A 6.1-kilobase insertion into the rudimentary (r) gene was cloned and partially sequenced. The insertion consists of a 703-base-pair (bp) P element next to a 5.4-kilobase single-copy sequence. The normal position of the single-copy sequence is near the tip of the X chromosome. Upon insertion into the r gene, this chimeric element generated an 8-bp target-site duplication, characteristic of P elements. At the non-P-element end of the insertion, the first 8 bp are identical to the first 8 bp of the inverted terminal repeats of the P element. Thus, this element has inverted terminal repeats of 8 bp. This large element can excise from the r gene under conditions of hybrid dysgenesis, which indicates that it behaves like a normal P element. These data support the conclusion that a normally stable single-copy sequence has now become unstable and duplicated within the genome.
Tsubota
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Department of Biology, University of Michigan, Ann Arbor 48109.
Huong
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D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D006824:Hybridization, Genetic; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D014960:X Chromosome
D004251:DNA Transposable Elements; D004247:DNA