Detection of antibodies against Bordetella avium in turkeys by avidin-biotin enhancement of the enzyme-linked immunosorbent assay and the dot-immunobinding assay.
An avidin-biotin-enhanced enzyme-linked immunosorbent assay (AB-ELISA) and an avidin-biotin-enhanced dot-immunobinding (AB-DIB) assay for detecting antibody to Bordetella avium in turkey sera were developed and compared with the microagglutination (MA) test. Whole-cell antigen, biotin-labeled goat anti-turkey IgG conjugate, and horseradish-peroxidase-labeled streptavidin were used in the AB-ELISA and AB-DIB assay. The AB-ELISA and AB-DIB assay were sensitive, specific, and reproducible. These assays were superior to the MA test for measuring acquired and maternal antibodies against B. avium. All MA-positive sera were positive by two assays, but some sera negative by MA test had titers in the AB-ELISA and AB-DIB assay. AB-ELISA and AB-DIB titers showed a positive correlation (r = 0.866), and AB-ELISA was more sensitive than the AB-DIB assay.
Tsai
H J
HJ
Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
Saif
Y M
YM
D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001360:Avidin; D001428:Bacterial Vaccines / Q000276:immunology; D001710:Biotin; D001884:Bordetella / Q000276:immunology*; D001885:Bordetella Infections / Q000175:diagnosis / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D011201:Poultry Diseases / Q000175:diagnosis*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms; D014422:Turkeys; D014611:Vaccination / Q000662:veterinary
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D001360:Avidin; D001710:Biotin