Role of PAF and cytokines in the modulation of Fc epsilon RII/CD23 expression on human eosinophils.
IL-4 and gamma IFN profoundly modulate the expression of the cell surface and soluble Fc epsilon RII/CD23 molecule, which is a low affinity Fc receptor for IgE having regulatory activity on immune system. IL-4 is a general inducer of Fc epsilon RII/CD23 on B lymphocyte, monocyte/macrophage, and eosinophil lineages, while gamma IFN counteracts the enhancing effect of IL-4 on normal B lymphocytes. However, the effect of gamma IFN is more complex than that of IL-4. gamma IFN up-regulates those on monoblast cell line U937 and eosinophil cell line EoL. This differential regulation of Fc epsilon RII/CD23 on B cells and the other cell types may play important roles in immune system, as macrophages and eosinophils are critical cells in the allergic reactions. In addition to cytokines, we found that platelet activation factor (PAF) up-regulates the expression of Fc epsilon RII/CD23, whereas transforming growth factor-beta (TGF-beta) and glucocorticosteroid (dexamethasone) down-regulate it on U937 and EOL cells. Variable and strong effect of these factors on the expression of CD23/Fc epsilon RII may indicate the involvement of this receptor system in the allergic as well as immune reactions and possibly the pathophysiology of the allergic and immunological disorders.
Kawabe
T
T
Virus Research Institute, Kyoto Univ., Japan.
Maeda
Y
Y
Maekawa
N
N
Tanaka
M
M
Mayumi
M
M
Mikawa
H
H
Yodoi
J
J
D015703:Antigens, CD / Q000097:blood*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000097:blood*; D015152:Blotting, Northern; D016207:Cytokines / Q000502:physiology*; D003907:Dexamethasone / Q000494:pharmacology; D004804:Eosinophils / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D010972:Platelet Activating Factor / Q000502:physiology*; D011961:Receptors, Fc / Q000097:blood*; D017455:Receptors, IgE; D011994:Recombinant Proteins / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000502:physiology
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D016207:Cytokines; D010972:Platelet Activating Factor; D011961:Receptors, Fc; D017455:Receptors, IgE; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D003907:Dexamethasone