Isolation and partial characterization of circulating immune complexes in sera of children with HBV-mediated glomerulonephritis.
Circulating immune complexes (CIC) containing HBsAg and HBeAg were identified in sera of 5 out of 6 children with hepatitis B mediated membranous glomerulonephritis. CIC were precipitated from sera by 3.5% PEG, washed and subsequently analysed after acid dissociation and trypsin digestion. HBsAg, anti-HBs and albumin; HBeAg, anti-HBe and anti-HBc were recovered from the isolated complexes and these findings are discussed. Analysis of 3.5% PEG mediated precipitate of human serum proteins showed the relatively high content of IgG classical pathway complement components: C1q, C4 and C3.
Gregorek
H
H
Department of Clinical Immunology, Children's Memorial Hospital, Warsaw.
Jung
A
A
Grabowska
B
B
Madaliński
K
K
D000293:Adolescent; D000936:Antigen-Antibody Complex / Q000097:blood* / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D003165:Complement System Proteins / Q000302:isolation & purification; D005260:Female; D015433:Glomerulonephritis, Membranous / Q000209:etiology / Q000276:immunology*; D006509:Hepatitis B / Q000150:complications* / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000097:blood / Q000302:isolation & purification; D006514:Hepatitis B Surface Antigens / Q000097:blood / Q000302:isolation & purification; D006513:Hepatitis B e Antigens / Q000097:blood / Q000302:isolation & purification; D006801:Humans; D008297:Male
D000936:Antigen-Antibody Complex; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D003165:Complement System Proteins