[Isolation of Cryptosporidium sp. oocysts from human fecal samples].
Experiments using two sequential discontinuous sucrose gradients, performed with 12%, 18%, 21% and 24% solutions, for the isolation of Cryptosporidium sp. oocysts from human fecal samples were undertaken. The sucrose gradients were centrifuged at 4000 rpm during 20 min. at 10 degrees C or room temperature. After that, 3 bands were observed. Oocysts were recovered mainly from the second band (75.5% after the first gradient, and 44.4% in the next). The two sequential discontinuous sucrose gradients would permit an efficient isolation of Cryptosporidium sp. oocysts from human fecal samples.
Mercado
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Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Chile, Santiago.
Araya
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Espinoza
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D000818:Animals; D002499:Centrifugation, Density Gradient; D003458:Cryptosporidium / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D006801:Humans; D007223:Infant
Aislamiento de ooquistes de Cryptosporidium sp. de muestras fecales humanas.