In vivo decreased expression of CD25 (p55 chain of IL-2 receptor) on CD3+ T cells correlates with low in vitro responsiveness to Plasmodium falciparum antigen in subjects living in a malaria endemic area.
Significant proliferative responses of peripheral blood mononuclear cells (PBMC) to crude Plasmodium falciparum schizont antigen (M.Ag) or purified recombinant 31.1 Ag (part of gp 195) were observed only in 46 and 39%, respectively, of 50 healthy subjects 5 to 63 years old living in Gabon, a malaria-endemic area. High responses to pokeweed mitogen were observed in all the subjects except one. Interferon-gamma (IFN-gamma) production paralleled the proliferative response, but in some subjects proliferation without a IFN-gamma response was observed. The proportion of subjects responding to M.Ag and 31.1 Ag increased with age. By cytofluorometric analysis performed with PBMC from 27 subjects, a substantial proportion of CD3+ T cells was found to bear the activation marker HLA-DR. However, the CD3+ cells expressed very low levels of CD25 (p55 chain of IL-2 receptor). The expression of CD25 on T cells and their capacity to respond to M.Ag were significantly correlated. In four subjects an increase in the percentage of CD3+ cells bearing the very late activation marker VLA-1 was observed.
Chizzolini
C
C
Centre International de Recherches Medicales, Franceville (CIRMF), Gabon.
Nicholson
J K
JK
Geinoz
A
A
Olsen-Rasmussen
M A
MA
Schrijvers
D
D
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000953:Antigens, Protozoan / Q000276:immunology*; D017252:CD3 Complex; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005681:Gabon; D006684:HLA-DR Antigens / Q000096:biosynthesis; D006801:Humans; D066298:In Vitro Techniques; D008288:Malaria / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010963:Plasmodium falciparum / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D015375:Receptors, Interleukin-2 / Q000276:immunology*; D011990:Receptors, Transferrin / Q000096:biosynthesis; D012044:Regression Analysis; D013601:T-Lymphocytes / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D000953:Antigens, Protozoan; D017252:CD3 Complex; D006684:HLA-DR Antigens; D011948:Receptors, Antigen, T-Cell; D015375:Receptors, Interleukin-2; D011990:Receptors, Transferrin