title: NMR investigation of the interaction of mithramycin A with d(ACCCGGGT)2.
abstract: The binding of mithramycin A to d(ACCCGGGT)2 has been investigated by one- and two-dimensional 1H NMR spectroscopy. Titration of the drug into the octamer solution results in loss of the oligonucleotide C2 symmetry at stoichiometric ratios less than 4 drug molecules per duplex. However, at a ratio of 4:1 (drug/duplex), the C2 symmetry of the oligonucleotide is restored. From these data it is evident that more than one complex forms at ratios less than 4:1 while only one complex predominates at the ratio 4:1. This is the first report of a DNA octamer which binds 4 large drug molecules. These results are compared to those we have recently reported for mithramycin binding to d(ATGCAT)2, where only a single, bound complex is observed, with a stoichiometry of 2:1.
authors.affiliation: Research School of Chemistry, Australian National University, Canberra.
authors.forename: M A
authors.initials: MA
authors.lastname: Keniry
authors.forename: D L
authors.initials: DL
authors.lastname: Banville
authors.forename: C
authors.initials: C
authors.lastname: Levenson
authors.forename: R H
authors.initials: RH
authors.lastname: Shafer
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D008926:Plicamycin / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D009838:Oligodeoxyribonucleotides; C066851:mithramycin A; D008926:Plicamycin