Isolation of cellular revertants from a rat cell line transformed by the E6 and E7 genes of human papillomavirus type 16.
Three revertants defective in the ability to form colonies in semisolid medium were isolated from a rat cell line transformed by the E6 and E7 genes of human papillomavirus type 16 (HPV16). These revertants appeared to be defective in a cellular factor(s) necessary for transformation by HPV16-E6E7 genes since they still expressed a comparable amount of HPV16-E6E7 mRNA and E7 protein to the parental cells, harbored rescuable transforming virus, and were resistant to retransformation by HPV16-E6E7 genes. All these reverted phenotypes of the three mutants were recessive on somatic cell hybridization with normal cells, because all the hybrids showed transformed phenotypes.
Sudiro
T M
TM
Department of Tumor Virology, Osaka University, Suita, Japan.
Inoue
H
H
Yutsudo
M
M
Hakura
A
A
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D002472:Cell Transformation, Viral; D015870:Gene Expression; D005814:Genes, Viral; D005816:Genetic Complementation Test; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D009857:Oncogenes; D027383:Papillomaviridae / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D012367:RNA, Viral / Q000235:genetics; D051381:Rats; D012097:Repressor Proteins
C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012367:RNA, Viral; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16