Serological and genomic characterization of L338, a novel equine group A rotavirus G serotype.
A group A rotavirus designated L338 was isolated from the faeces of a diarrhoeic foal and was compared to 11 standard G serotype strains of group A rotaviruses by cross-neutralization. It was clearly distinct from serotypes G1 to G11 and thus representative of a novel rotavirus G serotype tentatively designated G13. The nucleic acid sequence of the virion protein 7 (VP7) coding region was determined and the deduced amino acid sequence compared to published sequences. Within VP7 regions A and B, L338 was clearly distinct from serotypes G1 to G12 (excluding G7 which has not been sequenced), but region C was very similar to those of G3 and G8. This further questions the significance of region C in determining serotype specificity of at least three distinct rotavirus G serotypes.
Browning
G F
GF
Moredun Research Institute, Edinburgh, U.K.
Chalmers
R M
RM
Fitzgerald
T A
TA
Snodgrass
D R
DR
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D006736:Horses; D007106:Immune Sera; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping
D000956:Antigens, Viral; D036022:Capsid Proteins; D007106:Immune Sera; C050779:VP7 protein, Rotavirus