title: Nucleotide sequence of trpE, anthranilate synthase I gene, of Bacillus caldotenax.
abstract: trpE gene of Bacillus caldotenax was cloned by complementation of an Escherichia coli tryptophan auxotroph. The trpE encoding a 508 amino acid polypeptide is followed by trpD. S1-nuclease mapping of a trpE in vivo transcript suggested that a promoter preceding the trpE was utilized by E. coli. The deduced amino acid sequence of anthranilate synthase I (ASI) was compared with that of five other bacterials ASIs.
authors.affiliation: Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Shiratsuchi
authors.forename: S
authors.initials: S
authors.lastname: Sato
mesh_terms: D000595:Amino Acid Sequence; D000878:Anthranilate Synthase / Q000235:genetics* / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D016415:Sequence Alignment
chemical_list: D004269:DNA, Bacterial; D000878:Anthranilate Synthase