Effects of subminimal inhibitory concentrations of erythromycin, tetracycline, clindamycin, and minocycline on the neutrophil chemotactic factor production in Propionibacterium acnes biotypes 1-5.
Biotypes 1-5 Propionibacterium acnes (P. acnes) strains were grown in the presence of 1/10 minimal inhibitory concentrations (sub-MIC) of erythromycin (EM), tetracycline (TC), clindamycin (CLDM), or minocycline (MINO) and their culture filtrates were assayed for human neutrophil chemotactic activity using Boyden chamber methods. The addition of sub-MIC of MINO to the medium strongly suppressed the neutrophil chemotactic activity of the culture filtrates of P. acnes strains of all biotypes. In contrast, with sub-MIC of EM, TC, or CLDM, the activity of the culture filtrates of P. acnes strains of biotypes 2 and 3 were suppressed but those of biotypes 1, 2, and 5 were not. These results indicate that sub-MIC of MINO is capable of decreasing the inflammatory capacity of P. acnes strains of all biotypes.
Akamatsu
H
H
Department of Dermatology, Kansai Medical University, Osaka, Japan.
Nishijima
S
S
Takahashi
M
M
Ushijima
T
T
Asada
Y
Y
D000152:Acne Vulgaris / Q000188:drug therapy / Q000382:microbiology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002630:Chemotactic Factors / Q000096:biosynthesis*; D002981:Clindamycin / Q000494:pharmacology; D004917:Erythromycin / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests; D008911:Minocycline / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D011425:Propionibacterium acnes / Q000187:drug effects* / Q000254:growth & development; D013752:Tetracycline / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D002630:Chemotactic Factors; D002981:Clindamycin; D004917:Erythromycin; D013752:Tetracycline; D008911:Minocycline