Controlled production of cirrhosis in the rat liver.
In the present study we propose a standardization of the method for the induction of fibrosis of the liver in rats by weekly administration of CCl4 by the intragastric route. The present method is a variation of that described by Proctor and Chatamra. The initial CCl4 dose is 0.04 ml (X dose) but the subsequent doses are calibrated using a 5% variation in animal weight as a reference point. For a weight gain of 5% or more, the successive doses are progressively increased by 50 to 150% (1.5X, 2X, 2.5X). When there is no variation in weight or loss in less than 5%, the previous dose is maintained. For a weight loss of more than 5%, the CCl4 dose is decreased by 50%. Consistent fibrosis if obtained as early as during the 8th week. Collagen content increases progressively from the 8th to the 12th week, reaching on average a level eight-fold higher than that observed in the control.
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Laboratory of Hepatology, Federal University of Goiás School of Medicine, Brazil.
Parise
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Paranhos
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Braga
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Freitas
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Hidalgo
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Mor
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D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002251:Carbon Tetrachloride / Q000008:administration & dosage*; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D002251:Carbon Tetrachloride