Sequence specific purification of a particular tRNA by solid phase DNA probe.
A novel method for the purification of a specific tRNA using solid phase DNA probe is developed. With this method, the probe DNA immobilized on HPLC gel hybridized with target tRNA within a minute at room temperature. The hybridizing capacity of the solid phase probe was about 20 O.D. per gram dry gel when yeast phenylalanine tRNA was used. The specificity of this method was extremely high and the recovery rate was about 90%.
Tsurui
H
H
Department of Pathology, Juntendo University School of Medicine, Tokyo, Japan.
Kumazawa
Y
Y
Sanokawa
R
R
Watanabe
K
K
Wada
A
A
Shirai
T
T
D001483:Base Sequence; D015342:DNA Probes / Q000138:chemical synthesis; D007700:Kinetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012343:RNA, Transfer / Q000737:chemistry / Q000302:isolation & purification*; D013696:Temperature
D015342:DNA Probes; D012343:RNA, Transfer