title: N-Methyl-D-aspartate receptors and release of newly-synthesized [3H]dopamine in nucleus accumbens slices and its relationship with neocortical afferences.
abstract: 1. The effects of excitatory amino acid agonists on the release of newly-synthesized [3H]dopamine was examined in slices of nucleus accumbens of the rat. 2. L-glutamate and N-methyl-D-aspartate stimulated the release of newly-synthesized [3H]dopamine, which was completely inhibited by physiological concentrations of magnesium and by the selective and non-competitive antagonist MK-801. 3. Other ligands for excitatory amino acids subtype receptors, such as AMPA and kainic acid, had no effect of newly-synthesized [3H]dopamine release. 4. Frontal cortical ablation produced a significant increase on the N-methyl-D-aspartate-stimulated release of [3H]dopamine. 5. These data suggest that dopaminergic function in the rat nucleus accumbens is modulated by N-methyl-D-aspartate receptors, the sensitivity of which is determined, at least in part, by glutamatergic and/or aspartergic afferents from the frontal cortex.
authors.affiliation: Laboratory of Biochemical Pharmacology, Catholic University of Chile, Santiago.
authors.forename: H
authors.initials: H
authors.lastname: Asencio
authors.forename: G
authors.initials: G
authors.lastname: Bustos
authors.forename: K
authors.initials: K
authors.lastname: Gysling
authors.forename: R
authors.initials: R
authors.lastname: Labarca
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000344:Afferent Pathways / Q000502:physiology; D000596:Amino Acids / Q000502:physiology; D000818:Animals; D002540:Cerebral Cortex / Q000502:physiology*; D016291:Dizocilpine Maleate / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D016202:N-Methylaspartate / Q000378:metabolism; D009714:Nucleus Accumbens / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology*
chemical_list: D000596:Amino Acids; D016194:Receptors, N-Methyl-D-Aspartate; D016202:N-Methylaspartate; D016291:Dizocilpine Maleate; D011188:Potassium; D004298:Dopamine