Metabolic intermediates as potential regulators of glucose-6-phosphatase.
Twenty-five metabolites of glucose, gluconeogenic substrates, and related compounds were examined as potential inhibitors of glucose-6-phosphatase (EC 3.1.3.9) catalytic unit and substrate transport function, using disrupted and intact rat liver microsomes. Inhibitions (competitive) were noted with six. Calculated per cent inhibitions with presumed near-physiologic concentrations of inhibitor and substrate were small. However, when hepatic fructose-1-P concentration is elevated in response to a fructose load, inhibition of glucose-6-phosphatase by fructose-1-P may play a regulatory role, along with fructose-1-P-associated deinhibition of glucokinase, by directing glucose-6-P away from glucose formation and towards glycogen synthesis and glycolysis.
Robbins
B L
BL
Department of Biochemistry and Molecular Biology, University of North Dakota, School of Medicine, Grand Forks 58202.
Foster
J D
JD
Nordlie
R C
RC
D000818:Animals; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000494:pharmacology; D005952:Glucose-6-Phosphatase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats
D002241:Carbohydrates; D005952:Glucose-6-Phosphatase