title: The influence of extracellular magnesium on cell damage induced by ATP depletion in human fibroblasts.
abstract: Magnesium is an essential ion for the structural and functional integrity of cells. We have examined whether a low extracellular magnesium concentration contributes to cell damage induced by ATP depletion in a model system of quiescent fibroblasts. Cell damage assessed by the release of lactate dehydrogenase was slightly, but significantly increased in the absence of Mg2+. The increased cell damage may be caused by an increased Mg2+ loss during ATP depletion, by an altered cell membrane permeability for potassium and sodium, or by a disturbed balance between Mg2+ and Ca2+.
authors.affiliation: Department of Clinical Chemistry, Odense University Hospital, Denmark.
authors.forename: S R
authors.initials: SR
authors.lastname: Kristensen
authors.forename: M
authors.initials: M
authors.lastname: Hørder
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002463:Cell Membrane Permeability; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007460:Iodoacetamide / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology*; D010042:Ouabain / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
chemical_list: D000001:Calcimycin; D010042:Ouabain; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D000254:Sodium-Potassium-Exchanging ATPase; D008274:Magnesium; D002118:Calcium; D007460:Iodoacetamide