[Immune response induced by phosphofructokinase from E. histolytica in hamsters].
The enzymatic activity of inorganic pyrophosphate (PPi) dependent phosphofructokinase became manifest in the supernatant obtained by centrifugation in a homogenate of E. histolytica strain HMI-IMSS at 700,000 g. Partial purification of the enzyme was achieved by column chromatography with Ultrogel AcA-34. Ten protein elution spikes were obtained: five showed enzymatic activity. Elution spikes I and II attained the highest values of specific enzymatic activity 6.45 and 6.98 U/mg of protein, respectively. Next were spikes X and III with similar values 2.55 and 2.63 U/mg of protein, and spike IV presented the lowest value of 0.86 U/mg of protein. The five spikes were used to immunize hamsters which were challenged intrahepatically, four weeks later, with 3 x 10(5) trophozoites of E. histolytica. A control group of animals not immunized underwent intrahepatic challenge with the same number of amebae. The proteins with enzymatic activity contained in elution spikes I and II conferred immunologic protection in 100% of the animals, while elution spikes X and III were protective in 50 to 63%, and spike IV gave the lowest value of 37%. It can be assumed that there is an antienzyme antibody responsible for the absence of hepatic abscesses in the immunized hamsters.
Jiménez Cardoso
J M
JM
Unidad de Microscopía Electrónica, Facultad de Medicina, UNAM, México, D.F.
Jiménez
E
E
Kumate
J
J
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D000953:Antigens, Protozoan / Q000276:immunology* / Q000302:isolation & purification; D006224:Cricetinae; D004748:Entamoeba histolytica / Q000201:enzymology / Q000276:immunology*; D006790:Host-Parasite Interactions; D007114:Immunization; D008101:Liver Abscess, Amebic / Q000276:immunology / Q000473:pathology / Q000517:prevention & control; D008297:Male; D008647:Mesocricetus / Q000276:immunology*; D010732:Phosphofructokinase-1 / Q000276:immunology* / Q000302:isolation & purification
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D010732:Phosphofructokinase-1
Respuesta inmune inducida por la fosfofructoquinasa de E. histolytica en hámsters.