Protective effect of Ginkgo biloba extract (EGB 761) on free radical-induced changes in the electroretinogram of isolated rat retina.
The retina is a tissue particularly rich in polyunsaturated fatty acids and thus highly sensitive to lipid peroxidation initiated by oxygenated free radicals. By recording the electroretinogram (ERG) b wave amplitude on isolated rat retina, the authors have investigated the anti-oxidant properties of Ginkgo biloba extract (EGB 761). Two groups of rats were used: one group was treated with EGB 761 at a dose of 100 mg/kg/day per os for 10 days; the other one of untreated animals served as a control. At the end of the treatment (10 days), rats were sacrificed, one retina isolated and perfused in order to record ERG. Lipid peroxidation was induced by adding a mixture of (FeSO4 + Na ascorbate) to the perfusion solution. In the untreated rats a 50% decrease in ERG was observed after only 55 min. Such a delay in the decrease and subsequent maintenance of ERG b wave amplitude confirm that the anti-oxidant properties of EGB 761 can protect the retina against lipoperoxidation.
Droy-Lefaix
M T
MT
IHB/Ipsen Research Laboratories, Le Plessis-Robinson, France.
Bonhomme
B
B
Doly
M
M
D000818:Animals; D004596:Electroretinography; D016166:Free Radical Scavengers; D005609:Free Radicals; D020441:Ginkgo biloba; D066298:In Vitro Techniques; D010477:Perfusion; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D012160:Retina / Q000187:drug effects*; D000096983:Ginkgo Extract
D016166:Free Radical Scavengers; D005609:Free Radicals; D010936:Plant Extracts; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract