title: Increased expression of the laminin receptor in human colon cancer.
abstract: It has been proposed that among the various cell-surface proteins capable of interacting with laminin, the 67-kd high-affinity laminin receptor plays a crucial role during tumor invasion and metastasis. In this study, the expression of laminin-receptor-precursor messenger RNA (mRNA) and 67-kd protein was analyzed in human colon adenocarcinoma. In 22 of 23 patients with colon cancer, we found a 2- to 23-fold increase in levels of laminin-receptor-precursor mRNA in the cancer tissues compared with those in matched normal adjacent colonic mucosa. In 10 of 11 cases studied, the level of 67-kd laminin receptor, detected by affinity-purified anti-laminin-receptor synthetic peptide antibodies on immunoblots of matched tumor and normal tissue extracts, was higher in the colon carcinoma tissue. Immunodetection of laminin receptor in tissue sections using anti-laminin-receptor-peptide antibodies confirmed that the increased expression of laminin receptor was specifically associated with the cancer cells. In a series of 72 paraffin sections of colon lesions, we observed a correlation between the expression of the laminin receptor and the Dukes' classification. Our observations indicate that increased expression of laminin-receptor-precursor mRNA is associated with enhanced levels of the 67-kd laminin receptor as well as with the invasive phenotype of colon carcinoma. Detection of this metastasis-associated gene product may be a valuable adjunct in the evaluation of human colon cancer.
authors.affiliation: Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
authors.forename: V
authors.initials: V
authors.lastname: Cioce
authors.forename: V
authors.initials: V
authors.lastname: Castronovo
authors.forename: B M
authors.initials: BM
authors.lastname: Shmookler
authors.forename: S
authors.initials: S
authors.lastname: Garbisa
authors.forename: W F
authors.initials: WF
authors.lastname: Grigioni
authors.forename: L A
authors.initials: LA
authors.lastname: Liotta
authors.forename: M E
authors.initials: ME
authors.lastname: Sobel
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000648:ultrastructure*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015152:Blotting, Northern; D003110:Colonic Neoplasms / Q000378:metabolism / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism; D011971:Receptors, Immunologic / Q000235:genetics / Q000502:physiology*; D017474:Receptors, Laminin
chemical_list: D012333:RNA, Messenger; D011971:Receptors, Immunologic; D017474:Receptors, Laminin