Characterization and actions of human umbilical endothelium derived relaxing factor.
1. A bioassay cascade superfusion technique was utilized to study the properties of endothelium derived relaxing factor (EDRF) from human umbilical vein (HUV) and compare its actions on umbilical, chorionic plate and bovine pulmonary arterial strips. 2. Histamine (1 microM), bradykinin (1 microM) and A-23187 (0.3 microM, 1 microM) but not acetylcholine (1 microM) released EDRF. 3. The non-innervated human foetoplacental vessels, i.e., umbilical and chorionic plate arteries, do relax to EDRF by a guanosine 3':5'-cyclic monophosphate (cyclic GMP)-mediated mechanism. 4. The sensitivity of the human umbilical arterial strips to EDRF was less than that of the chronic plate arterial strips. Bovine pulmonary arterial strips were the most sensitive to the relaxant actions of human umbilical EDRF.
Chaudhuri
G
G
Department of Obstetrics & Gynecology, University of California, School of Medicine, Los Angeles 90024.
Buga
G M
GM
Gold
M E
ME
Wood
K S
KS
Ignarro
L J
LJ
D000818:Animals; D001158:Arteries / Q000187:drug effects / Q000502:physiology; D002417:Cattle; D002823:Chorion / Q000098:blood supply; D006152:Cyclic GMP / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D009569:Nitric Oxide / Q000378:metabolism / Q000494:pharmacology* / Q000502:physiology; D011651:Pulmonary Artery / Q000187:drug effects / Q000502:physiology; D014469:Umbilical Arteries / Q000187:drug effects / Q000502:physiology; D014471:Umbilical Veins / Q000378:metabolism; D014664:Vasodilation / Q000187:drug effects* / Q000502:physiology
D009569:Nitric Oxide; D006152:Cyclic GMP