Glycoprotein expression in mouse cerebellum: effects of inhibitors of N-linked glycosylation.
1. Explants of cerebellum from foetal mouse were cultured in vitro for 10 days in the presence of one of 4 inhibitors of N-linked glycosylation (castanospermine, deoxymannojirimycin, swainsonine, and tunicamycin). 2. The effects of the inhibitors were compared with respect to: (a) the activity of enzymes involved in glycoprotein biosynthesis and degradation; (b) the expression of N-linked glycoproteins; (c) the morphology and ultrastructure of the treated cerebellar explants.
Carroll
M
M
Department of Biochemistry, Queen Mary and Westfield College, London, England.
Bird
M M
MM
D017485:1-Deoxynojirimycin; D000818:Animals; D002531:Cerebellum / Q000378:metabolism* / Q000648:ultrastructure; D004622:Embryo, Mammalian / Q000033:anatomy & histology / Q000378:metabolism; D005944:Glucosamine / Q000031:analogs & derivatives / Q000494:pharmacology; D006023:Glycoproteins / Q000096:biosynthesis*; D006026:Glycoside Hydrolases / Q000032:analysis / Q000037:antagonists & inhibitors*; D006031:Glycosylation / Q000187:drug effects*; D006056:Golgi Apparatus / Q000648:ultrastructure; D007212:Indolizines / Q000494:pharmacology; D051379:Mice; D009924:Organ Culture Techniques; D013347:Subcellular Fractions / Q000737:chemistry; D017026:Swainsonine / Q000494:pharmacology; D014415:Tunicamycin / Q000494:pharmacology; D054836:Indolizidines
D017485:1-Deoxynojirimycin; D005944:Glucosamine; D006023:Glycoproteins; D006026:Glycoside Hydrolases; D007212:Indolizines; D017026:Swainsonine; D014415:Tunicamycin; C037806:castanospermine; D054836:Indolizidines