Inhibition of tumour necrosis factor alpha (TNF-alpha)-induced neutrophil respiratory burst by a TNF inhibitor.
Tumour necrosis factor alpha (TNF-alpha) plays an important role in microbial defence and tissue damage by activating neutrophils. Therefore the ability of natural molecules to regulate the activity of TNF-alpha is likely to be of major importance in our understanding of the mechanisms of inflammation. We have examined the effects of a highly purified urine-derived TNF inhibitor (TNF inh) on the TNF-alpha-induced respiratory burst in human neutrophils. TNF-alpha inh-treated TNF-alpha was unable to stimulate a neutrophil lucigenin-dependent chemiluminescence response and superoxide formation. Treatment of TNF with the inhibitor also significantly reduced the priming ability of TNF-alpha for a response to the peptide f-met-leu-phe. These results show that the ability of TNF-alpha to induce a key neutrophil response is amenable to regulation by the TNF-alpha inh.
Ferrante
A
A
Division of Immunology and Allergy, Hans Wilsdorf Laboratory, Hospital Cantonal University of Geneva, Switzerland.
Hauptmann
B
B
Seckinger
P
P
Dayer
J M
JM
D002478:Cells, Cultured; D006801:Humans; D008163:Luminescent Measurements; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D013481:Superoxides / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000276:immunology
D014409:Tumor Necrosis Factor-alpha; D013481:Superoxides