An Arabidopsis thaliana G-box-binding protein similar to the wheat leucine zipper protein identified as HBP-1.
G-box (CCACGTGG) like sequences are present in a variety of plant promoters and in many cases they have been demonstrated to be required for maximal expression of the corresponding gene. A nuclear protein, GBF, interacts specifically with the G-box motif of several RBCS and CAB promoters. Here we describe the isolation of a cDNA from Arabidopsis thaliana that encodes a protein, designated GBF-1, with DNA binding properties similar to GBF. GBF-1 is characterized by a basic/leucine zipper motif which is strikingly similar to the wheat protein identified as HBP-1. GBF-1 also interacts with an oligonucleotide derived from the wheat histone 3 promoter containing the binding site (hexamer, TGACGT) for HBP-1. This DNA element also contains a G-box-like motif, modification of which results in loss in binding of GBF-1.
Schindler
U
U
Department of Biology, University of Pennsylvania, Philadelphia 19104.
Ecker
J R
JR
Cashmore
A R
AR
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D050976:Basic-Leucine Zipper Transcription Factors; D001665:Binding Sites; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D051790:G-Box Binding Factors; D016350:Leucine Zippers / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors
D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; D051790:G-Box Binding Factors; D009687:Nuclear Proteins; D010940:Plant Proteins; D014157:Transcription Factors; C062189:histone DNA binding protein-1; D004247:DNA