Disease progression and viral genome variants in experimental feline leukemia virus-induced immunodeficiency syndrome.
A fatal immunodeficiency syndrome with clinical and pathologic features similar to human AIDS is inducible in cats by experimental inoculation with a specific strain of feline leukemia virus (FeLV) called FeLV-FAIDS. The course of the feline disease is characterized by an age-dependent prodromal period during which a non-disease-specific, common form of proviral DNA is detected in bone marrow. Preceding clinical onset of immunodeficiency is production of high levels of specific, pathogenic variant genomes, primarily as unintegrated viral DNA, in bone marrow. Acute immunodeficiency syndrome (survival period approximately 3 months) is associated with a short prodromal period and appearance of a characteristic variant genome (variant A) that persists at high copy number as integrated and full-length unintegrated viral DNA in bone marrow. Chronic immunodeficiency syndrome (survival greater than 1 year) is marked by a longer prodromal period, a more gradual onset of severe clinical immunosuppression, and a predominance of other variant genomes that often contain substantial internal deletions. In both forms of the disease, tissue-specific replication of certain variant viruses is noted in the bone marrow, intestine, and lymph nodes. Evidence from in vitro and in vivo virus transmission studies indicates that the appearance of FeLV-FAIDS variant viruses reflects differential replication of viral genomes pre-existing in the inoculum rather than rapid de novo evolution of new variants within each animal. These results demonstrate that retrovirus-induced immunodeficiency disease in cats can be associated with and prefigured by the amplified replication of specific viral variants in target tissues.
Mullins
J I
JI
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305-5402.
Hoover
E A
EA
Quackenbush
S L
SL
Donahue
P R
PR
D000818:Animals; D001853:Bone Marrow / Q000382:microbiology; D002415:Cats; D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis; D016181:Feline Acquired Immunodeficiency Syndrome / Q000150:complications / Q000382:microbiology*; D005814:Genes, Viral; D014644:Genetic Variation; D007422:Intestines / Q000382:microbiology; D007938:Leukemia / Q000382:microbiology; D005256:Leukemia Virus, Feline / Q000235:genetics* / Q000502:physiology; D008198:Lymph Nodes / Q000382:microbiology; D008223:Lymphoma / Q000382:microbiology; D009894:Opportunistic Infections / Q000150:complications; D011533:Proviruses / Q000235:genetics; D013047:Specific Pathogen-Free Organisms; D014779:Virus Replication
D004279:DNA, Viral