Inositol 1,4,5-trisphosphate receptor is phosphorylated by cyclic AMP-dependent protein kinase at serines 1755 and 1589.
IP3 activates intracellular calcium release by binding to an intracellular ligand gated calcium permeable channel which has been shown to be regulated by protein kinase A phosphorylation. Two consensus sequences for protein kinase A phosphorylation are predicted by the recently isolated cDNA of the mouse and rat. In the present study we have isolated and sequenced the two peptides in the rat IP3 receptor which are phosphorylated by protein kinase A and demonstrate protein kinase A phosphorylation on S-1755 and S-1589.
Ferris
C D
CD
Department of Neuroscience, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Cameron
A M
AM
Bredt
D S
DS
Huganir
R L
RL
Snyder
S H
SH
D000818:Animals; D015220:Calcium Channels; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D053496:Inositol 1,4,5-Trisphosphate Receptors; D046911:Macromolecular Substances; D010748:Phosphopeptides / Q000302:isolation & purification; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear; D012694:Serine; D014357:Trypsin
D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D046911:Macromolecular Substances; D010748:Phosphopeptides; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D012694:Serine; D015544:Inositol 1,4,5-Trisphosphate; D011494:Protein Kinases; D014357:Trypsin