title: Photo-CIDNP study of the interaction between the glucocorticoid receptor DNA-binding domain and glucocorticoid response elements.
abstract: Photo-CIDNP studies were performed on two protein fragments that both contain the double zinc-finger DNA-binding domain of the glucocorticoid receptor. In the absence of DNA, Tyr452 and Tyr474 are polarised in both fragments while Tyr497 is not. Addition of a palindromic glucocorticoid response element (GRE) results in the suppression of Tyr474 polarization while the polarization of Tyr452 is unaffected. The same result is observed upon adding a half GRE to the protein fragment indicating that the suppression of Tyr474 polarization is not due to protein-protein contacts but to interaction with DNA.
authors.affiliation: Department of Chemistry, University of Utrecht, The Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: Kellenbach
authors.forename: T
authors.initials: T
authors.lastname: Härd
authors.forename: R
authors.initials: R
authors.lastname: Boelens
authors.forename: K
authors.initials: K
authors.lastname: Dahlman
authors.forename: J
authors.initials: J
authors.lastname: Carlstedt-Duke
authors.forename: J A
authors.initials: JA
authors.lastname: Gustafsson
authors.forename: G A
authors.initials: GA
authors.lastname: van der Marel
authors.forename: J H
authors.initials: JH
authors.lastname: van Boom
authors.forename: B
authors.initials: B
authors.lastname: Maler
authors.forename: K R
authors.initials: KR
authors.lastname: Yamamoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004396:Coloring Agents / Q000528:radiation effects; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010446:Peptide Fragments / Q000378:metabolism*; D010777:Photochemistry; D011485:Protein Binding; D011487:Protein Conformation; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D016335:Zinc Fingers
chemical_list: D004396:Coloring Agents; D004268:DNA-Binding Proteins; D010446:Peptide Fragments; D011965:Receptors, Glucocorticoid; D004247:DNA