Methylation-dependent and -independent DNA binding of nuclear factor EF-C.
Nuclear factor EF-C binds to important functional sites in the hepatitis B virus and polyomavirus enhancer regions. In this paper, we have characterized new and divergent EF-C binding sites in several viral regulatory regions. We also have demonstrated that EF-C binds to certain DNA sites only when CpG dinucleotide base pairs are methylated (m5C). EF-C binds to other sites in a methylation-independent manner. Based on similar binding properties and identical binding sites, it is very likely that EF-C corresponds to the nuclear protein MDBP previously identified by virtue of binding to methylated DNA.
Garcia
A D
AD
Department of Microbiology, State University of New York, Stony Brook 11794.
Ostapchuk
P
P
Hearing
P
P
D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics*; D004247:DNA / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D015967:Gene Expression Regulation, Viral; D006801:Humans; D066298:In Vitro Techniques; D008745:Methylation; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D012045:Regulatory Sequences, Nucleic Acid; D018139:Simplexvirus / Q000235:genetics*; D014157:Transcription Factors
D004279:DNA, Viral; D004268:DNA-Binding Proteins; C066141:MBD1 protein, human; D009687:Nuclear Proteins; D014157:Transcription Factors; D004247:DNA