title: Effect of subinhibitory concentrations of clindamycin and trospectomycin on the adherence of Staphylococcus epidermidis in an in vitro model of vascular catheter colonization.
abstract: Septicemia, often due to Staphylococcus epidermidis, is a life-threatening complication associated with indwelling vascular catheters. An important factor in the development of such infections is glycocalix, or slime. An in vitro model that mimics intravenous delivery systems in humans was developed. It consisted of a modified Robbins device containing slices of silicone catheters in the removable ports, through which S. epidermidis diluted in 5% dextrose-normal saline with 10% heat-inactivated normal human serum was run, with and without clindamycin and trospectomycin. S. epidermidis was recovered from all catheters in the absence of antibiotics; no growth was detected with antibiotics. Scanning electron microscopy demonstrated significant reduction in glycocalix and no visible organisms with all concentrations except 0.5 micrograms/ml trospectomycin and 1 microgram/ml clindamycin; for those, a moderate amount of glycocalix and a few bacteria were seen. Thus, subinhibitory levels of trospectomycin and clindamycin may have a role in the prevention of microbial adherence to vascular catheters.
authors.affiliation: Department of Medical Specialties, University of Texas, M.D. Anderson Cancer Center, Houston.
authors.forename: N
authors.initials: N
authors.lastname: Khardori
authors.forename: E
authors.initials: E
authors.lastname: Wong
authors.forename: H
authors.initials: H
authors.lastname: Nguyen
authors.forename: C
authors.initials: C
authors.lastname: Jeffery-Wiseman
authors.forename: E
authors.initials: E
authors.lastname: Wallin
authors.forename: R P
authors.initials: RP
authors.lastname: Tewari
authors.forename: G P
authors.initials: GP
authors.lastname: Bodey
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D002408:Catheters, Indwelling; D002981:Clindamycin / Q000494:pharmacology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D013010:Sonication; D000198:Spectinomycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D000900:Anti-Bacterial Agents; D011135:Polysaccharides, Bacterial; D002981:Clindamycin; C043267:trospectomycin; D000198:Spectinomycin