[Isolation and purification of human brain creatine kinase (CK-BB)].
A improved method for isolation and purification of CK-BB from human brain is introduced. CK-BB of fraction IV was purified to approximately 306.9 fold as compared with homogenate of human brain. The specific activity was 322.2 IU/mg protein. The recovery of creatine kinase activity was 52.8%. This fraction was analyzed by SDS-PAGE. It showed a single major band with an estimated molecular weight of 43000 when it stained with coomassie blue. Purity of fraction V was much better than fraction IV, but some CK activity was lost.
Lu
Z
Z
Institute of Cardiovascular Diseases, Beijing.
D001921:Brain / Q000201:enzymology*; D002624:Chemistry, Clinical; D003402:Creatine Kinase / Q000302:isolation & purification*; D006801:Humans; D007527:Isoenzymes
D007527:Isoenzymes; D003402:Creatine Kinase