title: Pathways of activation of the Epstein-Barr virus productive cycle.
abstract: The promoter for the 2.8-kb RNA of Epstein-Barr virus encoding BZLF1 and BRLF1 was identified and shown to be activated by both BZLF1 and BRLF1 but not by 12-O-tetradecanoylphorbol-13-acetate. Site-directed mutagenesis suggests that two binding sites for BZLF1 within the promoter contribute to the transactivation by BZLF1. The early kinetics of induction of the 2.8- and 1.0-kb RNAs encoding BZLF1 and BRLF1 in Akata cells treated with anti-immunoglobulin indicate that both RNAs appear within 60 min. The results indicate some likely pathways of activation of Epstein-Barr virus productive cycle gene expression.
authors.affiliation: Ludwig Institute for Cancer Research, St. Mary's Hospital Medical School, Paddington, London, England.
authors.forename: A J
authors.initials: AJ
authors.lastname: Sinclair
authors.forename: M
authors.initials: M
authors.lastname: Brimmell
authors.forename: F
authors.initials: F
authors.lastname: Shanahan
authors.forename: P J
authors.initials: PJ
authors.lastname: Farrell
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000254:growth & development*; D017874:Immediate-Early Proteins; D007700:Kinetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000096:biosynthesis; D012260:Ribonucleases / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors; D014764:Viral Proteins; D014775:Virus Activation
chemical_list: C068382:BRLF1 protein, Human herpesvirus 4; C063676:BZLF1 protein, Herpesvirus 4, Human; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D017874:Immediate-Early Proteins; D012367:RNA, Viral; D015534:Trans-Activators; D014157:Transcription Factors; D014764:Viral Proteins; D012260:Ribonucleases; D013755:Tetradecanoylphorbol Acetate