title: Chronic inflammation induces the expression of dendritic cell markers not related to functional antigen presentation on peritoneal exudate macrophages.
abstract: Phenotypic heterogeneity between macrophages in situ has been reported, using different macrophage-specific monoclonal antibodies. Microenvironmental factors, which may play an important role in inducing this heterogeneity were studied in a local inflammatory response in vivo. Our results show that peritoneal exudate macrophages, formed during Thioglycollate induced chronic inflammation, acquire expression of the dendritic cell markers NLDC-145 and 6D2. In contrast to these phenotypic characteristics, NLDC-145+/6D2+ exudate cells do not function like dendritic cells in an allogeneic-MLR, but are suppressive when compared with steady state peritoneal cells. The expression of these membrane markers on exudate cells is discussed.
authors.affiliation: Department of Cell Biology, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: J F
authors.initials: JF
authors.lastname: Wijffels
authors.forename: Z
authors.initials: Z
authors.lastname: de Rover
authors.forename: N
authors.initials: N
authors.lastname: van Rooijen
authors.forename: G
authors.initials: G
authors.lastname: Kraal
authors.forename: R H
authors.initials: RH
authors.lastname: Beelen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D001202:Ascitic Fluid / Q000166:cytology; D015415:Biomarkers; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D016130:Immunophenotyping; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010538:Peritonitis / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology; D013864:Thioglycolates
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015415:Biomarkers; D000949:Histocompatibility Antigens Class II; D013864:Thioglycolates