The timing of late signals for initiation of the cell cycle in fibroblasts.
Quiescent mouse (3T3C) fibroblasts were stimulated to proliferate by adding mouse epidermal growth factor (EGF) or recombinant human basic fibroblast growth factor (bFGF). A maximum rate of DNA synthesis was observed 19 h after addition of the growth factor, irrespective of the concentration used. If the growth factor was removed from the cells within 9 h of addition, DNA synthesis was not observed; if it was left for longer periods, a progressive increase in maximum rate of DNA synthesis occurred. If the growth factor was removed at 9 h for a period of time, the reduction in maximal rate of DNA synthesis increased with the length of time for which the growth factor was absent. Added EGF substituted completely for removed bFGF and vice versa.
Chana
A K
AK
Department of Biochemistry, University of Liverpool, UK.
Smith
J A
JA
D016475:3T3 Cells / Q000166:cytology*; D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000008:administration & dosage / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D051379:Mice; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D015398:Signal Transduction / Q000502:physiology*
D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D004247:DNA