Purification and characterization of human muscle glutathione S-transferases: evidence that glutathione S-transferase zeta corresponds to a locus distinct from GST1, GST2, and GST3.
Human muscle glutathione S-transferase isozyme, GST zeta (pI 5.2) has been purified by three different methods using immunoaffinity chromatography, DEAE cellulose chromatography, and isoelectric focusing. GST zeta prepared by any of the three methods does not recognize antibodies raised against the alpha, mu, or pi class glutathione S-transferases of human tissues. GST zeta has a blocked N-terminus and its peptide fingerprints also indicate it to be distinct from the alpha, mu, or pi class isozymes. As compared to GSTs of alpha, mu, and pi classes, GST zeta displays higher activities toward t-stilbene oxide and Leukotriene A4 methyl ester. GST zeta also expresses GSH-peroxidase activity toward hydrogen peroxide. The Kms of GST zeta for CDNB and GSH were comparable to those reported for other human GSTs but its Vmax for CDNB, 7620 mol/mol/min, was found to be considerably higher than that reported for other human GSTs. The kinetics of inhibition of GST zeta by hematin, bile acids, and other inhibitors also indicate that it was distinct from the three classes of GST isozymes. These studies suggest that GST zeta corresponds to a locus distinct from GST1, GST2, and GST3 and probably corresponds to the GST4 locus as suggested previously by Laisney et al. (1984, Human Genet. 68, 221-227). The results of peptide fingerprints and kinetic analysis indicate that as compared to the pi and alpha class isozymes, GST zeta has more structural and functional similarities with the mu class isozymes. Besides GST zeta several other GST isozymes belonging to pi and mu class have also been characterized in muscle. The pi class GST isozymes of muscle have considerable charge heterogeneity among them despite identical N-terminal sequences.
Singhal
S S
SS
Department of Human Biological Chemistry & Genetics, University of Texas Medical Branch, Galveston 77550.
Ahmad
H
H
Sharma
R
R
Gupta
S
S
Haque
A K
AK
Awasthi
Y C
YC
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology; D001647:Bile Acids and Salts / Q000494:pharmacology; D002845:Chromatography; D005982:Glutathione Transferase / Q000276:immunology / Q000302:isolation & purification*; D006427:Hemin / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000276:immunology / Q000302:isolation & purification; D007700:Kinetics; D017572:Leukotriene A4; D015289:Leukotrienes / Q000494:pharmacology; D008969:Molecular Sequence Data; D009132:Muscles / Q000187:drug effects / Q000201:enzymology*; D013267:Stilbenes / Q000494:pharmacology; D013379:Substrate Specificity
D000906:Antibodies; D001647:Bile Acids and Salts; D007527:Isoenzymes; D017572:Leukotriene A4; D015289:Leukotrienes; D013267:Stilbenes; D006427:Hemin; D006861:Hydrogen Peroxide; D005982:Glutathione Transferase; C025906:stilbene oxide