Isolation, characterization, and sequence of an Escherichia coli heat shock gene, htpX.
We isolated and characterized a new Escherichia coli gene, htpX. The htpX gene has been localized at min 40.3 on the chromosome. We determined its transcription and translation start site. htpX expresses a 32-kDa protein from a monocistronic transcript; expression of this protein is induced by temperature upshift. htpX is expressed from a sigma 32-dependent promoter and is thus part of the heat shock regulon. Cells carrying a htpX gene disruption grow well at all temperatures and under all conditions tested and have no apparent phenotype. However, cells which overexpress a truncated form of the protein display a higher rate of degradation of puromycyl peptides.
Kornitzer
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Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Teff
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Altuvia
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Oppenheim
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D000595:Amino Acid Sequence; D010582:Bacteriophage lambda; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000502:physiology; D006358:Hot Temperature; D008242:Lysogeny / Q000187:drug effects; D045726:Metalloproteases; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics; D011506:Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D015183:Restriction Mapping
D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D011506:Proteins; D012333:RNA, Messenger; C068607:HtpX protein, E coli; D045726:Metalloproteases