trkB, a neural receptor protein-tyrosine kinase: evidence for a full-length and two truncated receptors.
We have screened an adult rat cerebellar cDNA library in search of novel protein tyrosine-kinase (PTK) cDNAs. A cDNA for a putative PTK, trkB, was cloned, and its sequence indicates that it is likely to be derived from a gene for a ligand-regulated receptor closely related to the human trk oncogene. Northern (RNA) analysis showed that the trkB gene is expressed predominantly in the brain and that trkB expresses multiple mRNAs, ranging from 0.7 to 9 kb. Hybridization of cerebral mRNAs with a variety of probes indicates that there are mRNAs encoding truncated trkB receptors. Two additional types of cDNA were isolated, and their sequences are predicted to encode two distinct C-terminally truncated receptors which have the complete extracellular region and transmembrane domain, but which differ in their short cytoplasmic tails.
Middlemas
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DS
Molecular Biology and Virology Laboratory, Salk Institute, San Diego, California 92138.
Lindberg
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RA
Hunter
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T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009424:Nervous System Physiological Phenomena; D015345:Oligonucleotide Probes; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011956:Receptors, Cell Surface / Q000235:genetics*; D020813:Receptor, trkB
D004247:DNA; D008562:Membrane Glycoproteins; D015345:Oligonucleotide Probes; D011505:Protein-Tyrosine Kinases; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C064371:Ntrk2 protein, mouse; D020813:Receptor, trkB