Control by treatment with lithium chloride of ornithine decarboxylase in Ehrlich ascites tumor cells.
The activity of ornithine decarboxylase (ODC) was increased in Ehrlich ascites tumor cells by a change of the medium. This increase in the activity was inhibited by the addition of LiCl to the medium. Na+ and Mg2+ did not affect the enzyme activity. The inhibition of the enzyme activity with LiCl was not reversed by the addition of inositol or dibutyryl cyclic AMP. Total RNA was isolated from cells treated with LiCl and the relative abundance of the ODC mRNA was measured by Northern blot analysis. These levels in cells treated with LiCl were comparable to those in control cells. In the treated cells, the biological half-life of ODC was 14 min, which was the same as for the control cells. The inhibition by LiCl of ODC activity was not due to the nonspecific toxicity of LiCl. These results suggest that treatment of Ehrlich ascites tumor cells with LiCl suppressed ODC induction during translation, not during transcription or after translation.
Matsui-Yuasa
I
I
Department of Biochemistry, Osaka City University Medical School, Japan.
Tsuruta
D
D
Uemoto
M
M
Hasuma
T
T
Morisawa
S
S
Otani
S
S
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D002712:Chlorides / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008094:Lithium / Q000494:pharmacology*; D018021:Lithium Chloride; D009955:Ornithine Decarboxylase / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D002712:Chlorides; D012333:RNA, Messenger; D008094:Lithium; D009955:Ornithine Decarboxylase; D018021:Lithium Chloride; D013755:Tetradecanoylphorbol Acetate