title: Haplotyping by double PCR amplification of specific alleles.
abstract: Haplotypes are useful in population genetics and medicine. Determining haplotypes in the absence of DNA samples from appropriate family members can be difficult and laborious. We have developed a rapid and reproducible method for haplotyping an individual in the absence of relatives. The method utilizes pairs of allele-specific PCR primers to differentially amplify each haplotype. Four amplifications can distinguish the haplotypes produced by two biallelic polymorphisms.
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic/Foundation, Rochester, MN 55905.
authors.forename: G
authors.initials: G
authors.lastname: Sarkar
authors.forename: S S
authors.initials: SS
authors.lastname: Sommer
mesh_terms: D000483:Alleles; D001483:Base Sequence; D004247:DNA; D006239:Haplotypes; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D011954:Receptors, Dopamine / Q000235:genetics; D017448:Receptors, Dopamine D2; D015203:Reproducibility of Results
chemical_list: D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D004247:DNA