Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives.
A family of cloning vectors derived from plasmid pACYC184 and, therefore, compatible with pBR322 and its derivatives (especially the pUC family of vectors), is described. They all contain a multiple cloning site (MCS) and the lacZ alpha reporter gene for easy cloning. They have been grouped in three sets: (i) six of the vectors contain a chloramphenicol-resistance (CmR)-encoding gene and each a different MCS with 16 unique restriction sites overall; (ii) another six vectors contain a kanamycin-resistance (KmR)-encoding gene and the same six MCS; and (iii) two CmR vectors that contain the SP6 and T7 promoters flanking the MCS and lacZ alpha reporter gene of pUC18/19.
Bartolomé
B
B
Departamento de Biología Molecular, Universidad de Cantabria, Santander, Spain.
Jubete
Y
Y
Martínez
E
E
de la Cruz
F
F
D001483:Base Sequence; D002702:Chloramphenicol Resistance / Q000235:genetics; D005822:Genetic Vectors / Q000502:physiology*; D007613:Kanamycin Resistance / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics
D001616:beta-Galactosidase