Solubilization of UDP-glucose collagen glucosyltransferase activities from chick embryo liver: Golgi apparatus, smooth and rough endoplasmic reticulum.
1. The choice of a suitable detergent for solubilization of UDP-glucose collagen glucosyltransferase (GGT) activities from chick embryo liver has been investigated. Several detergents were used (zwitterionic detergent as Chaps, and non-ionic detergents as Triton X-100, Nonidet P 40, Brij 35). 2. All the detergents with GGT activities were tested in Golgi apparatus, smooth and rough endoplasmic reticulum (SER, RER). 3. 80-100% GGT Golgi apparatus activity was easily solubilized at low concentrations in surfactant (0.5 mg/ml). 25-78% of SER and RER GGT activities were extracted at this concentration. 4. A higher level of detergent (5 mg/ml) was necessary to release all GGT activities of SER and RER. Protein extraction was identical to GGT activities.
Bortolato
M
M
Laboratoire de Biochemie des Membranes (LBTM CNRS UM 380024), Université Claude Bernard Lyon I, Villeurbanne, France.
Azzar
G
G
Farjanel
J
J
Got
R
R
D000818:Animals; D002642:Chick Embryo; D002793:Cholic Acids; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D005109:Extracellular Matrix / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism*; D006056:Golgi Apparatus / Q000201:enzymology*; D006801:Humans; D008099:Liver / Q000201:enzymology*; D017830:Octoxynol; D011092:Polyethylene Glycols; D011347:Procollagen / Q000378:metabolism*; D012995:Solubility
D002793:Cholic Acids; D003902:Detergents; D011347:Procollagen; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D005964:Glucosyltransferases; C023247:UDP glucose-collagen glucosyltransferase; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate