Sindbis virus nsP1 functions in negative-strand RNA synthesis.
A mutation at nucleotide 1101 of Sindbis virus ts11 nsP1 caused temperature-sensitive negative-strand synthesis and suppressed the 24R phenotype, which is caused by a mutation in nsP4. Nonstructural proteins synthesized and accumulated by ts11 at 40 degrees C did not cause the reactivation of negative-strand synthesis upon return to 30 degrees C and did not prevent the formation of new replication complexes at 30 degrees C.
Wang
Y F
YF
Department of Microbiology, Medical College of Ohio, Toledo 43699-0008.
Sawicki
S G
SG
Sawicki
D L
DL
D000818:Animals; D002213:Capsid / Q000235:genetics*; D002460:Cell Line; D007700:Kinetics; D009154:Mutation; D010641:Phenotype; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics*; D012845:Sindbis Virus / Q000235:genetics*; D013489:Suppression, Genetic; D013696:Temperature; D013698:Templates, Genetic; D014162:Transfection; D014758:Viral Core Proteins / Q000235:genetics*; D017361:Viral Nonstructural Proteins
D012367:RNA, Viral; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins