title: The ArsR protein is a trans-acting regulatory protein.
abstract: The arsR gene encodes the regulatory protein of the plasmid-encoded arsenical resistance operon. A series of in-frame fusions was constructed between the C-terminally truncated arsR gene and the coding region for the mature form of beta-lactamase (blaM). Fusions containing most of the arsR gene were still inducible by arsenicals. Fusions containing less than 102 residues of the 117-residue ArsR protein were constitutive. When a wild-type arsR gene was placed in trans, the constitutive constructs were again inducible. The results demonstrate that the ArsR protein is a trans-acting regulatory protein which controls its own expression.
authors.affiliation: Department of Biochemistry, Wayne State University School of Medicine, Detroit, Michigan 48201.
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: B P
authors.initials: BP
authors.lastname: Rosen
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D000595:Amino Acid Sequence; D001151:Arsenic / Q000494:pharmacology; D053501:Arsenite Transporting ATPases; D018053:Arsenites; D001426:Bacterial Proteins; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000235:genetics*; D005809:Genes, Regulator / Q000235:genetics; D015151:Immunoblotting; D016623:Ion Pumps; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D009876:Operon / Q000235:genetics; D011815:R Factors / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism
chemical_list: C489527:ArsR protein, E coli; D018053:Arsenites; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D016623:Ion Pumps; D009097:Multienzyme Complexes; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D001618:beta-Lactamases; D000251:Adenosine Triphosphatases; D053501:Arsenite Transporting ATPases; C015001:arsenite; D001151:Arsenic