Novel method of synthesis of oligo(deoxyribonucleoside phosphorothioates).
The syntheses of 5'-O-DMT base-protected nucleoside 3'-O-[2-thio-1.3.2-oxathia-phospholanes] (1) and their subsequent reactions with nucleosides bound via 3'-oxygen to solid support, performed in the presence of DBU, formulate the basis to new methodology of preparation of title compounds--OligoS. Moreover separation of 1 into diastereoisomeric species allows the preparation of OligoS in stereocontrolled manner.
Stec
W J
WJ
Polish Academy of Sciences, Department of Bioorganic Chemistry, Lódź.
D002845:Chromatography; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D013873:Thionucleotides / Q000138:chemical synthesis* / Q000737:chemistry
D009838:Oligodeoxyribonucleotides; D013873:Thionucleotides