title: Studies on properties of thermostable beta-amylase from Thermoactinomyces sp.V4.
abstract: A thermostable beta-amylase from Thermoactinomyces sp. V4 isolated from soil is reported. The enzyme has optimum activity at 70 degrees C and pH6. It is stable at 50 degrees C (4h) and retains 92% of activity at 55 degrees C (2h). The extent of hydrolysis soluble starch is about 77% and the product is identified as beta-maltose by paper chromatography and rotatory examination. Sulfhydryl reagent has no effect on the beta-amylase activity.
authors.affiliation: Shanghai Institute of Plant Physiology, Chinese Academy of Sciences.
authors.forename: B Y
authors.initials: BY
authors.lastname: Zhou
authors.forename: Y X
authors.initials: YX
authors.lastname: Zheng
mesh_terms: D001420:Bacteria, Aerobic / Q000201:enzymology; D002854:Chromatography, Paper; D004795:Enzyme Stability; D006094:Gram-Positive Bacteria / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007517:Isoamylase / Q000378:metabolism; D012988:Soil Microbiology; D012996:Solutions; D013213:Starch / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D013696:Temperature; D001614:beta-Amylase / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D012996:Solutions; D013438:Sulfhydryl Compounds; D013213:Starch; D001614:beta-Amylase; D007517:Isoamylase