Replication of lambda plasmid in amino acid-starved strains of Escherichia coli.
We found that lambda plasmid replication, as measured by the increase in plasmid content per bacterial mass, proceeds for hours in an amino acid-starved, relaxed mutant of Escherichia coli K-12, whereas is inhibited in its wild-type stringent partner. Replication of lambda plasmid in amino acid-starved, relaxed cells reveals absolute lambda O dependence and is not inhibited by chloramphenicol at 200 micrograms/ml. The replication also occurs in wild-type cells treated with chloramphenicol. We conclude that lambda plasmid replication is under stringent control, probably as a result of the action of ppGpp, the signal for the stringent response, on RNA polymerase.
Wegrzyn
G
G
Department of Molecular Biology, University of Gdańsk, Poland.
Kwaśnik
E
E
Taylor
K
K
D000596:Amino Acids / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics*; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D005784:Gene Amplification; D007532:Isoleucine / Q000235:genetics; D010957:Plasmids / Q000235:genetics*
D000596:Amino Acids; D007532:Isoleucine