Purification of a high-affinity inositol 1,3,4,5-tetrakisphosphate receptor from brain.
Ins(1,3,4,5)P4 has been suggested to be involved in cellular Ca2+ signalling. A receptor from pig cerebellar membranes, which binds InsP4 with high affinity and selectivity [Donié & Reiser (1989) FEBS Lett. 254, 155-158], has been solubilized and purified about 20,000-fold by chromatography using CM-cellulose, heparin-agarose and hydroxyapatite. The InsP4 receptor, identified by SDS/PAGE, had an apparent molecular mass of 42 kDa and bound 4.6 nmol of InsP4 per mg of protein, with a dissociation constant of 5.6 nM.
Donié
F
F
Physiologisch-Chemisches Institut, Universität Tübingen, Federal Republic of Germany.
Reiser
G
G
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism*; D002845:Chromatography / Q000379:methods; D002846:Chromatography, Affinity / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D017886:Durapatite; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006882:Hydroxyapatites; D007295:Inositol Phosphates / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D011956:Receptors, Cell Surface / Q000302:isolation & purification* / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear; D013552:Swine
D006882:Hydroxyapatites; D007295:Inositol Phosphates; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; C061107:inositol-1,3,4,5-tetrakisphosphate receptor; D017886:Durapatite