A simple procedure for the enzymic digestion of edible tissues prior to detection of drug residues.
A simple procedure for the enzymic digestion of edible tissues is described and compared with other procedures. The samples are digested overnight in an enzyme suspension containing subtilisin A at 60 degrees C and pH 9. The resulting digest contains only a few small tissue fragments. This method is suitable for routine analysis, since the manipulation of the samples is very limited.
Daeseleire
E
E
Laboratory of Food Analysis, State University of Ghent, Belgium.
De Guesquière
A
A
Van Peteghem
C
C
D000818:Animals; D002417:Cattle; D004350:Drug Residues / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D003012:Microbial Collagenase / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D013381:Subtilisins / Q000378:metabolism*
D013381:Subtilisins; D003012:Microbial Collagenase