Differential expression of phenylalanine ammonia-lyase and chalcone synthase during soybean nodule development.
We have used conserved and nonconserved regions of cDNA clones for phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS) isolated from a soybean-nodule cDNA library to monitor the expression of members of the two gene families during the early stages of the soybean-Bradyrhizobium japonicum symbiosis. Our results demonstrate that subsets of the PAL and CHS gene families are specifically induced in soybean roots after infection with B. japonicum. Furthermore, by analyzing a supernodulating mutant line of soybean that differs from the wild-type parent in the number of successful infections, we show that the induction of PAL and CHS is related to postinfection events. Nodulated roots formed by a Nod+ Fix- strain of B. japonicum, resembling a pathogenic association, display induction of another distinct set of PAL and CHS genes. Our results suggest that the symbiosis-specific PAL and CHS genes in soybean are not induced by stress or pathogen interaction.
Estabrook
E M
EM
Department of Agronomy, New Mexico State University, Las Cruces 88003.
Sengupta-Gopalan
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D000217:Acyltransferases / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D005419:Flavonoids / Q000096:biosynthesis; D005786:Gene Expression Regulation; D015964:Gene Expression Regulation, Bacterial; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D009586:Nitrogen Fixation; D010650:Phenylalanine Ammonia-Lyase / Q000096:biosynthesis* / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D012230:Rhizobiaceae / Q000235:genetics; D016415:Sequence Alignment; D013025:Glycine max / Q000201:enzymology* / Q000502:physiology; D013559:Symbiosis
D005419:Flavonoids; D008565:Membrane Proteins; D010940:Plant Proteins; C045625:nodulin; D000217:Acyltransferases; C020141:flavanone synthetase; D010650:Phenylalanine Ammonia-Lyase