title: Isolation and characterization of Bacillus subtilis mutants blocked in the synthesis of pantothenic acid.
abstract: We have produced and characterized by physiological and enzymatic analyses pantothenate (pan) auxotrophs of Bacillus subtilis. panB auxotrophs are deficient in ketopantoate hydroxymethyltransferase, whereas panE mutants lack ketopantoic acid reductase. The pan mutations were mapped by phage PBS1-mediated two-factor crosses and found to be located in the interval purE-tre of the genetic map of B. subtilis.
authors.affiliation: Departamento de Microbiologia, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Republica Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Baigori
authors.forename: R
authors.initials: R
authors.lastname: Grau
authors.forename: H R
authors.initials: HR
authors.lastname: Morbidoni
authors.forename: D
authors.initials: D
authors.lastname: de Mendoza
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics*; D001412:Bacillus subtilis / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D019877:Hydroxymethyl and Formyl Transferases; D016296:Mutagenesis; D010205:Pantothenic Acid / Q000096:biosynthesis*; D011995:Recombination, Genetic; D014166:Transferases / Q000235:genetics
chemical_list: D010205:Pantothenic Acid; D000429:Alcohol Oxidoreductases; C021934:2-dehydropantoate 2-reductase; D014166:Transferases; D019877:Hydroxymethyl and Formyl Transferases; C021477:3-methyl-2-oxobutanoate hydroxymethyltransferase