title: Localization of insulin-like growth factor-II and hepatitis B virus mRNAs and proteins in human hepatocellular carcinomas.
abstract: We have previously demonstrated increased levels of insulin-like growth factor (IGF)-II mRNAs and protein with re-expression of a fetal pattern of transcripts in human hepatocarcinoma. In the present study, we have investigated IGF-II transcripts and protein in liver tissues from patients with hepatocarcinoma infected with hepatitis B virus, by using in situ hybridization and immunohistochemical techniques. The IGF-II transcripts and protein have been localized to the hepatocytes, be they normal or tumoral with a gradient for IGF-II expression from normal to dysplastic and tumoral tissues. Hepatitis B virus mRNAs and viral surface antigen have only been detected in some hepatocytes in the peritumoral tissues. Therefore, the results show expression of IGF-II in hepatocytes. The increase of IGF-II expression in tumor hepatocytes support the hypothesis that it might represent a marker of hepatocytes differentiation.
authors.affiliation: INSERM U.75, Chu Necker, Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Lamas
authors.forename: B
authors.initials: B
authors.lastname: Le Bail
authors.forename: C
authors.initials: C
authors.lastname: Housset
authors.forename: O
authors.initials: O
authors.lastname: Boucher
authors.forename: C
authors.initials: C
authors.lastname: Bréchot
mesh_terms: D000911:Antibodies, Monoclonal; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D006515:Hepatitis B virus / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000276:immunology / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D006514:Hepatitis B Surface Antigens; D015347:RNA Probes; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D007335:Insulin-Like Growth Factor II