title: Ethanol-induced focal cell necrosis via microcirculatory disturbance in the perfused rat liver.
abstract: In the perfused rat liver, infusion of ethanol induced an initial increase in portal pressure which is an indicator of vasoconstriction and a subsequent increase in lactic dehydrogenase (LDH) release, which is an indicator of cell damage in a dose-dependent fashion. Simultaneous infusion of sodium nitroprusside, a potent vasodilator, (100 microM) inhibited the increases in portal pressure and LDH release. Focal hepatocellular necrosis evidenced by trypan-blue stained cell nuclei were localized in midzonal and pericentral area of the liver lobules at 60 min after ethanol load. These ethanol-induced microcirculatory disturbance might be involved in the pathogenesis of alcoholic liver disease.
authors.affiliation: First Department of Medicine, Osaka University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Oshita
authors.forename: N
authors.initials: N
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Yoshihara
authors.forename: Y
authors.initials: Y
authors.lastname: Takei
authors.forename: S
authors.initials: S
authors.lastname: Kashio
authors.forename: T
authors.initials: T
authors.lastname: Hijioka
authors.forename: H
authors.initials: H
authors.lastname: Fukui
authors.forename: M
authors.initials: M
authors.lastname: Goto
authors.forename: T
authors.initials: T
authors.lastname: Matsunaga
authors.forename: S
authors.initials: S
authors.lastname: Okumura
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity*; D006519:Hepatitis, Alcoholic / Q000473:pathology / Q000503:physiopathology; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008099:Liver / Q000098:blood supply* / Q000187:drug effects / Q000473:pathology; D008297:Male; D009336:Necrosis; D009599:Nitroprusside / Q000494:pharmacology*; D011168:Portal System / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014661:Vasoconstriction / Q000187:drug effects
chemical_list: D009599:Nitroprusside; D000431:Ethanol; D007770:L-Lactate Dehydrogenase