[Existence of atrial natriuretic peptide in choroid, retina and ciliary body in rabbits].
The presence of atrial natriuretic factor (ANF) in choroid, retina and ciliary body of rabbit eyes has been studied. The peptide was extracted by reverse phase chromatography using Sep-Pak C18 cartridges. Immunoreactive ANF (Ir-ANF) was measured by a specific RIA. In the choroid, retina and ciliary body the ir-ANF concentrations were 444 +/- 94, 67 +/- 12 and 223 +/- 75 pg/g tissue weight (n = 16), respectively. The biological activity of the acid extract ANF was evaluated by a 125I-ANF binding assay to a retinal particulate preparation. Results demonstrate the existence of biological active ANF in choroid, retina and ciliary body of rabbit eyes. The presence of ANF and its specific receptors in the ocular tissues suggests that this hormone might play a physiological role in the intraocular fluid homeostasis.
García de Lacoba
M
M
Centro de Diabetes e Hipertensión, Departamento de Medicina Interna III, Hospital Universitario San Carlos, Madrid, España.
Fernández-Durango
R
R
Triviño
A
A
Ramírez
J M
JM
Ramírez
A I
AI
Salazar
J J
JJ
Fernández-Cruz
A
A
Gutkowska
J
J
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis*; D002829:Choroid / Q000737:chemistry*; D002924:Ciliary Body / Q000737:chemistry*; D005260:Female; D011817:Rabbits; D012160:Retina / Q000737:chemistry*
D009320:Atrial Natriuretic Factor
Existencia del péptido atrial natriurético en coroides, retina y cuerpo ciliar de conejo.