title: The effect of inositol 1,4,5-trisphosphate and inositol 1,4,5-trisphosphorothioate on calcium release and membrane skeleton organization in the human red blood cell.
abstract: The inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] content of unstimulated human red blood cells (RBC) has been found to be 227 +/- 83 pmol of Ins(1,4,5)P3 per mL of packed cells. Ins (1,4,5)P3 at concentrations between 0.4-5.0 microM releases Ca2+ from RBC plasma membrane with an EC50 of 0.55 microM. Inositol 4,5-bisphosphate [Ins(4,5)P2] is less potent, but inositol 1-phosphate [Ins(1)P], inositol 1,4-bisphosphate [Ins (1,4)P2], and adenosine triphosphate (ATP) are inactive. The release was stereospecific for D-Ins(1,4,5)P3; 1 microM L-Ins(1,4,5)P3 released no more Ca2+ than the control. A nonhydrolyzable analog of Ins(1,4,5)P3, inositol 1,4,5-trisphosphorothioate [Ins(1,4,5)PS3] evokes sustained release of Ca2+ from isolated ghosts. Release of 45Ca2+ was also observed after the addition of AlF4-. Ionophore A23187 and AlF4- increase the level of Ins(1,4,5)P3 in intact RBC to 1004 +/- 533 and 455 +/- 74 pmol/mL of packed RBC, respectively. We have elaborated a method for visualization of spectrin by indirect immunofluorescence in smears of RBC. Ins(1,4,5)P3 evokes shape changes in permeabilized RBC and disorganization of the spectrin network. The shape changes are stereospecific for the D-enantiomer, since L-Ins(1,4,5)P3 and other compounds had no effect. Whereas the effect of Ins(1,4,5)P3 was reversible, as was the weaker effect of Ins(4,5)P2, Ins(1,4,5)PS3 evoked irreversible shape changes. Shape changes and spectrin disorganization were also observed after the action of AlF4- and ionophore A23187. We conclude that the phosphoinositide signaling system plays an important role in the shape maintenance of human RBC.
authors.affiliation: Department of Physiology and Developmental Biology, Faculty of Sciences, Charles University, Prague, Czechoslovakia.
authors.forename: A
authors.initials: A
authors.lastname: Strunecká
authors.forename: N I
authors.initials: NI
authors.lastname: el Desouki
authors.forename: J
authors.initials: J
authors.lastname: Palecek
authors.forename: E
authors.initials: E
authors.lastname: Kmonícková
authors.forename: L
authors.initials: L
authors.lastname: Krpejsová
authors.forename: B V
authors.initials: BV
authors.lastname: Potter
mesh_terms: D000535:Aluminum / Q000494:pharmacology; D017607:Aluminum Compounds; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000097:blood*; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000648:ultrastructure; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D005459:Fluorides; D005461:Fluorine / Q000494:pharmacology; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000097:blood / Q000494:pharmacology / Q000502:physiology* / Q000031:analogs & derivatives; D007295:Inositol Phosphates / Q000494:pharmacology*; D009946:Organothiophosphorus Compounds / Q000494:pharmacology*; D013049:Spectrin / Q000032:analysis
chemical_list: D017607:Aluminum Compounds; D007295:Inositol Phosphates; D009946:Organothiophosphorus Compounds; C054737:inositol 1,4,5-triphosphorothioate; D013049:Spectrin; C050992:tetrafluoroaluminate; D005461:Fluorine; D000001:Calcimycin; D015544:Inositol 1,4,5-Trisphosphate; D000535:Aluminum; D005459:Fluorides; D002118:Calcium