Molecular genetic approaches to the cytoskeleton in Dictyostelium.
Recent advances in molecular genetic techniques are being applied in Dictyostelium to test and expand prevailing views on the functioning of the actin-based cytoskeleton. Current research involves the disruption, by homologous recombination, of genes encoding cytoskeletal elements. We suggest combining classical and molecular genetic approaches to supplement these investigations.
Patterson
B
B
Department of Cell Biology, Stanford University, CA 94305.
Ruppel
K M
KM
Spudich
J A
JA
D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000378:metabolism; D003599:Cytoskeleton / Q000648:ultrastructure*; D004023:Dictyostelium / Q000235:genetics* / Q000648:ultrastructure; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005821:Genetic Techniques; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism
D000199:Actins; D003598:Cytoskeletal Proteins; D005656:Fungal Proteins; D008840:Microfilament Proteins