[TNF-alpha synthesis by cells from bronchioloalveolar lavage and by circulating mononuclear cells in recipients of heart-lung transplantation].
In 10 patients who received a heart lung transplant, TNF-alpha generation by cells collected during bronchioloalveolar lavages (n = 30) and by circulating mononuclear cells was measured. Basal and recombinant IL-2-stimulated productions (50 U/ml) were measured. TNF-alpha concentration was determined by an immunoradiometric assay (IRMA). Circulating mononuclear cells produced at least 4 times less TNF-alpha than BAL cells. Rejection episodes or CMV diseases were not associated with significant changes in TNF-alpha generation. Recombinant IL-2 increased this production in both cell populations but the magnitude of this effect was smaller in BAL cells, suggesting an in vivo preactivation.
Rondeau
E
E
Service de Néphrologie, Hôpital Tenon, Paris.
Cerrina
J
J
Delarue
F
F
Ladurie
F L
FL
Hervé
P
P
Chapelier
A
A
Dartevelle
P
P
Sraer
J D
JD
D000328:Adult; D001773:Blood Cells / Q000187:drug effects / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D005260:Female; D006084:Graft Rejection / Q000502:physiology*; D016041:Heart-Lung Transplantation / Q000009:adverse effects*; D006801:Humans; D015592:Immunoradiometric Assay; D007376:Interleukin-2 / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D011184:Postoperative Period; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000096:biosynthesis
D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha
Synthèse de TNF-alpha par les cellules du lavage bronchiolo-alvéolaire et les cellules mononucléées circulantes chez les transplantés coeur-poumon.