title: Solid phase synthesis of mRNA by the phosphoramidite approach using 2'-O-1-(2-chloroethoxy)ethyl protection and its stability in E. coli system.
abstract: The decaoligoribonucleotides containing initiation codon AUG of the phage O beta-A protein mRNA were synthesized on a solid phase by the phosphoramidite approach using 2'-O-1-(2-chloroethoxy)ethyl (Cee) protection. The Cee group is completely stable under the acidic conditions required to remove the 5'-terminal protecting groups in oligoribonucleotide synthesis on a solid support, and yet is easily removable at pH 2.0 for the final unblocking step. Stabilization of the synthetic mRNA to in the cell-free translation system from E. coli A19 was measured. It was found that the oligomers with selected 2'-O-methylation were degraded completely in the translation system.
authors.affiliation: Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Sakatsume
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: H
authors.initials: H
authors.lastname: Takaku
mesh_terms: D001483:Base Sequence; D003062:Codon; D004926:Escherichia coli / Q000378:metabolism*; D004987:Ethers; D006868:Hydrolysis; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds; D012333:RNA, Messenger / Q000138:chemical synthesis*
chemical_list: D003062:Codon; D004987:Ethers; D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D009943:Organophosphorus Compounds; D012333:RNA, Messenger