Comparative studies of the colony-promoting activity of porcine kidney extract with several interleukins and colony-stimulating factors.
Porcine kidney extracts (PKE) possess colony-promoting activity (CPA) which stimulates primitive hematopoietic cells in the presence of granulocyte/macrophage colony-stimulating factor (GM-CSF), but PKE itself does not stimulate colony formation on murine bone marrow cells. We have compared the CPA of PKE with that of recombinant cytokines or CSFs such as interleukin-1 alpha (IL-1 alpha), IL-3, IL-6, granulocyte colony-stimulating factor (G-CSF), GM-CSF and macrophage colony-stimulating factor (CSF-1). All of these factors were less potent than PKE. Furthermore, the combinations of IL-1 alpha or PKE with G-CSF, GM-CSF, IL-3 or IL-6 were examined in the presence of one of these factors such as CSF. It is found that PKE acts synergistically with G-CSF, GM-CSF, IL-3 and IL-6, showing enhancement ratios of 10, 2.5, 4.2 and 30, respectively. The combination of IL-1 alpha resulted in poor colony formation in contrast with those of PKE, except for CSF-1. These results suggest that the CPA of the factor(s) in PKE differ from the cytokines and CSFs tested in this study, and is significantly affected by various types of CSF.
Kashiwakura
I
I
Hokkaido Institute of Pharmaceutical Sciences, Otaru, Japan.
Hayase
Y
Y
Takagi
Y
Y
D000009:Abdominal Muscles / Q000502:physiology; D000818:Animals; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D007378:Interleukins / Q000494:pharmacology*; D007668:Kidney / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D013552:Swine; D014020:Tissue Extracts / Q000494:pharmacology*
D003115:Colony-Stimulating Factors; D007378:Interleukins; D014020:Tissue Extracts