title: Comparison of antibody response by use of synthetic adjuvant system and Freund complete adjuvant in rabbits.
abstract: Two commercially available synthetic adjuvant systems, trehalose dimycolate (TDM) and TDM + monophosphoryl lipid A (MPL), were compared with Freund complete adjuvant (FCA) for the ability to stimulate antibody production in New Zealand White rabbits (Oryctolagus cuniculus). In addition, each animal was evaluated for adverse reactions. The antigen, rat liver microsomal epoxide hydrolase, was administered SC emulsified with FCA, TDM, or TDM + MPL. Serum antibody titers were stimulated with all 3 adjuvant-antigen combinations. The highest titer was produced by use of FCA; TDM + MPL produced an intermediate response, and TDM produced the lowest titer. All of the rabbits immunized with FCA developed sterile subcutaneous abscesses. Rabbits immunized with TDM or TDM + MPL developed no abscesses, and only slight reactions at the injection site. The synthetic adjuvant system TDM + MPL is recommended for use in rabbits, considering its adequate stimulation of antibody production with minimal adverse reactions.
authors.affiliation: Department of Laboratory Animal Science, SmithKline & French Laboratories, King of Prussia, PA 19406.
authors.forename: F M
authors.initials: FM
authors.lastname: Mallon
authors.forename: M E
authors.initials: ME
authors.lastname: Graichen
authors.forename: B R
authors.initials: BR
authors.lastname: Conway
authors.forename: M S
authors.initials: MS
authors.lastname: Landi
authors.forename: H C
authors.initials: HC
authors.lastname: Hughes
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation; D003311:Cord Factors / Q000276:immunology; D004851:Epoxide Hydrolases / Q000276:immunology; D005620:Freund's Adjuvant / Q000494:pharmacology*; D008050:Lipid A / Q000031:analogs & derivatives / Q000276:immunology; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D011817:Rabbits / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D013047:Specific Pathogen-Free Organisms
chemical_list: D000276:Adjuvants, Immunologic; D003311:Cord Factors; D008050:Lipid A; D005620:Freund's Adjuvant; D004851:Epoxide Hydrolases; C048436:monophosphoryl lipid A