The nicotinic acetylcholine receptor channel in embryonic muscle of Xenopus laevis. A program to separate channel types.
The single channel activity of nicotinic acetylcholine receptor (AChR) channels from Xenopus laevis embryonic myotomes in culture, was studied with the patch clamp technique. Two types of single channel activity were observed at low agonist concentration (100 nM ACh-Cl), which were distinguished by their conductance (60 and 40 pS, approximately). Both channel types were recorded simultaneously and therefore to carry out the kinetic analysis, we separate them by using a program exploiting the difference in amplitude of the channels. By using the program CLASS the stationary kinetic characteristics of both types of channels can be obtained separately and evaluated.
Rojas
L
L
Centro de Biología Celular, Facultad de Ciencias, Universidad Central de Venezuela, Caracas.
Senquiz
J
J
Zuazaga
C
C
D000818:Animals; D004553:Electric Conductivity; D004594:Electrophysiology; D007700:Kinetics; D009132:Muscles / Q000196:embryology* / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000502:physiology*; D012984:Software; D014982:Xenopus laevis / Q000196:embryology*
D011978:Receptors, Nicotinic