Interstitial cell proliferation in the testis of the ethylene dimethane sulfonate-treated rat.
This study was conducted to examine interstitial cell proliferation in the testis of the ethylene dimethane sulfonate (EDS)-treated rat. Initial autoradiographic studies demonstrated a peak of [3H]thymidine incorporation by interstitial cells at 2 and 4 days post-EDS treatment. Subsequent studies were designed using in vivo pulse labeling regimens in an attempt to identify interstitial cell proliferation associated with Leydig cell regeneration. Rats were injected with [3H]thymidine at days 2 and 4 post-EDS and were killed 6 hours later or at 30 days post-EDS. Although cells labeled at 2 and 4 days post-EDS appeared to undergo subsequent division, the Leydig cells visible at 30 days post-EDS were not labeled. In a second study, rats were injected with [3H]thymidine at days 10 and 20 post-EDS and were killed either 6 hours later or at 24 days post-EDS. In the 10-day post-EDS group, interstitial cells were labeled at both the 6-hour and 24-day time points; however, Leydig cells present at 24 days were not labeled. In contrast, the testes of rats that were killed at 20 days post-EDS (6 hours labeling period) contained Leydig cells that displayed grains over the nucleus, thus suggesting that Leydig cell proliferation had occurred. In addition, a high number of the Leydig cells observed at 24 days post-EDS were labeled, suggesting that they arose from divisions occurring during the 20- to 24-day post-EDS period. These studies demonstrate that interstitial cell proliferation occurs in several stages following EDS treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
Myers
R B
RB
Department of Physiology and Endocrinology, Medical College of Georgia, Augusta 30912.
Abney
T O
TO
D000818:Animals; D001345:Autoradiography; D002455:Cell Division / Q000187:drug effects; D006063:Chorionic Gonadotropin / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D007985:Leydig Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008698:Mesylates / Q000494:pharmacology*; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000166:cytology* / Q000187:drug effects
D006063:Chorionic Gonadotropin; D008698:Mesylates; D004247:DNA; C002994:ethylene dimethanesulfonate