Experimental therapy of human glioma by means of a genetically engineered virus mutant.
Malignant gliomas are the most common malignant brain tumors and are almost always fatal. A thymidine kinase-negative mutant of herpes simplex virus-1 (dlsptk) that is attenuated for neurovirulence was tested as a possible treatment for gliomas. In cell culture, dlsptk killed two long-term human glioma lines and three short-term human glioma cell populations. In nude mice with implanted subcutaneous and subrenal U87 human gliomas, intraneoplastic inoculation of dlsptk caused growth inhibition. In nude mice with intracranial U87 gliomas, intraneoplastic inoculation of dlsptk prolonged survival. Genetically engineered viruses such as dlsptk merit further evaluation as novel antineoplastic agents.
Martuza
R L
RL
Molecular Neurogenetics Laboratory, Harvard Medical School, Massachusetts General Hospital-East, Charlestown 02129.
Malick
A
A
Markert
J M
JM
Ruffner
K L
KL
Coen
D M
DM
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D001932:Brain Neoplasms / Q000628:therapy*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D017245:Foscarnet; D005910:Glioma / Q000628:therapy*; D051379:Mice; D008819:Mice, Nude; D016296:Mutagenesis; D010746:Phosphonoacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D018139:Simplexvirus / Q000235:genetics / Q000276:immunology*; D013937:Thymidine Kinase / Q000235:genetics; D014740:Vidarabine / Q000494:pharmacology; D014765:Viral Vaccines / Q000627:therapeutic use*
D000998:Antiviral Agents; D014765:Viral Vaccines; D017245:Foscarnet; D013937:Thymidine Kinase; D014740:Vidarabine; D010746:Phosphonoacetic Acid