Lung beta-adrenoceptors in pulmonary hypertension. A study of biopsy specimens in children with congenital heart disease.
Characteristics of beta-adrenoceptors were analyzed using radioligand-binding techniques with 3H-dihydroalprenolol in lung specimens from 11 children with pulmonary hypertension (median age, three years) undergoing surgical repair of congenital heart defects and four pediatric control subjects (median age, five years) undergoing thoracotomy for removal of neoplasms or cysts. Scatchard analysis of 3H-DHA binding to lung membranes showed similar values of the dissociation constant in both groups (Kd = 0.72 +/- 0.22 nM in patients vs 1.22 +/- 0.22 nM in controls; p = NS). The receptor density was significantly increased in patients in comparison with controls, with respective values of 164 +/- 19 and 95 +/- 13 fmol/mg of protein (p less than 0.025), and correlated directly with mean pulmonary arterial pressure (r = 0.82; p less than 0.0005). No significant relationship was observed between receptor number and pulmonary arterial medial thickness. Thus, the increase in receptor density in these patients may be related to adaptative changes in cells other than vascular smooth muscle.
Lopes
A A
AA
Department of Pediatric Cardiology, Heart Institute, School of Medicine, University of São Paulo, Brazil.
Liberato
M H
MH
Brentani
M M
MM
Aiello
V D
VD
Riso
A A
AA
Ebaid
M
M
D000293:Adolescent; D001706:Biopsy; D001794:Blood Pressure / Q000502:physiology; D001981:Bronchial Arteries / Q000378:metabolism / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D004082:Dihydroalprenolol / Q000378:metabolism; D006330:Heart Defects, Congenital / Q000150:complications*; D006801:Humans; D006976:Hypertension, Pulmonary / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D007223:Infant; D008168:Lung / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D011943:Receptors, Adrenergic, beta / Q000302:isolation & purification / Q000378:metabolism*; D013045:Species Specificity; D014316:Tritium
D011943:Receptors, Adrenergic, beta; D014316:Tritium; D004082:Dihydroalprenolol