title: Sequential enzymatic synthesis and biodistribution of radiolabelled inositol and inositol analogs.
abstract: The desired radiolabelled inositol (Ins) and inositol-1-phosphate (IP) were obtained from radiolabelled glucose by sequential enzyme reactions in a short time. Rapid separation and fractionation of enzymes and labelled products from the reaction mixture was achieved by HPLC with a gel-permeation chromatography column. Examination of the biodistribution of radiolabelled Ins, IP and their acetylated analogs suggested that intact Ins labelled with 11C would be more effective than 11C-labelled acetylated Ins as a brain diagnostic agent for positron emission tomographic studies of the metabolism of phosphatidylinositol and its role as a second messenger in the brain.
authors.affiliation: Department of Radiopharmacy, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: I
authors.initials: I
authors.lastname: Ogihara-Umeda
authors.forename: S
authors.initials: S
authors.lastname: Kojima
authors.forename: H
authors.initials: H
authors.lastname: Nishigori
mesh_terms: D000107:Acetylation; D000818:Animals; D001921:Brain / Q000378:metabolism; D002250:Carbon Radioisotopes; D007294:Inositol / Q000096:biosynthesis / Q000493:pharmacokinetics*; D007295:Inositol Phosphates / Q000493:pharmacokinetics; D008297:Male; D051379:Mice; D014018:Tissue Distribution
chemical_list: D002250:Carbon Radioisotopes; D007295:Inositol Phosphates; D007294:Inositol