The inhibitory effects of oligonucleotides, complementary to Marek's disease virus mRNA transcribed from the BamHI-H region, on the proliferation of transformed lymphoblastoid cells, MDCC-MSB1.
An oligonucleotide complementary to the splice donor sequence of the 1.8 kb gene family produced from the BamHI-H region of Marek's disease virus (MDV) DNA inhibited the proliferation of the MDV-derived lymphoblastoid cell line, MDCC-MSB1 (MSB-1), but not that of the avian lymphoid leukosis-derived lymphoblastoid cell line, LSCC-BK3. Colony formation in soft agar was also inhibited by treatment of MSB-1 cells with the antisense oligonucleotide. It is hypothesized that expression of the 1.8 kb gene family produced from the BamHI-H region is directly associated with the maintenance of the tumorigenic state of transformed Marek's disease-derived lymphoblastoid cells.
Kawamura
M
M
Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Hayashi
M
M
Furuichi
T
T
Nonoyama
M
M
Isogai
E
E
Namioka
S
S
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002645:Chickens; D004279:DNA, Viral / Q000032:analysis; D008381:Herpesvirus 2, Gallid / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014779:Virus Replication / Q000187:drug effects
D004279:DNA, Viral; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D012367:RNA, Viral