Alpha 1-adrenergic stimulation is coupled to cardiac myocyte hypertrophy.
We have investigated whether alpha 1-adrenergic stimulation is coupled to myocardial hypertrophy in adult rat ventricular cells (ARVC) in culture. Exposure of ARVC to norepinephrine (NE) caused a significant increase in [3H]-leucine incorporation and total protein content in ARVC. Prazosin totally inhibited the NE-stimulated leucine uptake by ARVC. Inhibition of spontaneous contraction of ARVC with KCl or verapamil did not prevent the stimulation of hypertrophy by NE. Protooncogene c-myc expression in ARVC was induced at 1 h after exposure to NE. Above results support the premise that activation of the alpha 1-adrenergic receptors by NE induces hypertrophy of ARVC, independent of changes in contractile activity.
Ikeda
U
U
Department of Cardiology, Jichi Medical School, Tochigi, Japan.
Tsuruya
Y
Y
Yaginuma
T
T
D000818:Animals; D002478:Cells, Cultured; D006352:Heart Ventricles; D006984:Hypertrophy; D007930:Leucine / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D009638:Norepinephrine / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D011942:Receptors, Adrenergic, alpha / Q000502:physiology*; D014700:Verapamil / Q000494:pharmacology
D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D011942:Receptors, Adrenergic, alpha; D011189:Potassium Chloride; D014700:Verapamil; D007930:Leucine; D009638:Norepinephrine