N-acetylglucosaminyltransferase from Ascaris suum.
The occurrence of N-acetylglucosaminyltransferase, the initial step in the synthesis of the carbohydrate moiety of N-linked glycoproteins, is demonstrated in the microsomal fraction of the nematode Ascaris suum. Phosphatidylglycerol stimulated enzyme activity three- to six-fold without affecting the Km values of either substrates, uridinediphospho-N-acetylglucosamine or dolichylphosphate. The Km values were determined to be about 12 microM and 100 micrograms ml-1, respectively. The enzyme activity was strongly inhibited by tunicamycin acting as a competitive inhibitor with respect to the substrate uridinediphospho-N-glucosamine.
Kyossev
Z
Z
Bulgarian Academy of Sciences, Central Laboratory of Helminthology, Sofia.
Bergmann
B
B
Ossikovski
E
E
Walter
R D
RD
D000818:Animals; D001200:Ascaris / Q000201:enzymology*; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D005964:Glucosyltransferases / Q000032:analysis* / Q000037:antagonists & inhibitors; D008861:Microsomes / Q000201:enzymology; D017351:N-Acetylglucosaminyltransferases; D010715:Phosphatidylglycerols / Q000494:pharmacology; D014415:Tunicamycin / Q000494:pharmacology
D010715:Phosphatidylglycerols; D014415:Tunicamycin; D005964:Glucosyltransferases; D017351:N-Acetylglucosaminyltransferases; C037649:N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase