title: Genetic analysis of a meiotic recombination hotspot on chromosome III of Saccharomyces cerevisiae.
abstract: In a previous study, we analyzed meiotic recombination events that occurred in the 22-kb region (LEU2 to CEN3) of chromosome III of Saccharomyces cerevisiae. We found one region with an enhanced level of crossovers (a hotspot) and one region with a depressed level of crossovers. In this study, we show that about one-third of the crossovers that occur between LEU2 and CEN3 are initiated in a 1.3-kb region located approximately 6 kb from the centromere. Both crossovers and gene conversion events are initiated at this site. Events initiated at this position can be resolved as crossovers in regions located either centromere-distally or centromere-proximally from the initiation site.
authors.affiliation: Institute of Cancer Research, Columbia University, New York, New York 10032.
authors.forename: L S
authors.initials: LS
authors.lastname: Symington
authors.forename: A
authors.initials: A
authors.lastname: Brown
authors.forename: S G
authors.initials: SG
authors.lastname: Oliver
authors.forename: P
authors.initials: P
authors.lastname: Greenwell
authors.forename: T D
authors.initials: TD
authors.lastname: Petes
mesh_terms: D001483:Base Sequence; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000648:ultrastructure*; D003434:Crossing Over, Genetic / Q000235:genetics; D005785:Gene Conversion / Q000235:genetics; D005819:Genetic Markers; D005822:Genetic Vectors; D008540:Meiosis; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*
chemical_list: D005819:Genetic Markers