title: Stable growth of simian virus 40 recombinants containing multimerized enhancers.
abstract: Multiple copies of each of three genetically defined simian virus 40 protoenhancers, A, B, and C, were able to substitute for the wild-type simian virus 40 enhancer. Although the recombinant viruses grew poorly, they could be propagated without the accumulation of enhancer rearrangements that might improve viability. Mutations that inactivate the multimerized B and C protoenhancers abolished virus growth, but, unexpectedly, a mutation that inactivates the octamer-enhanson within the B protoenhancer increased virus viability. This positive effect may reflect loss of repression of the B protoenhancer by the ubiquitous octamer-motif-binding protein Oct-1.
authors.affiliation: Cold Spring Harbor Laboratory, New York 11724.
authors.forename: B
authors.initials: B
authors.lastname: Ondek
authors.forename: W
authors.initials: W
authors.lastname: Herr
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004742:Enhancer Elements, Genetic; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D015183:Restriction Mapping; D013539:Simian virus 40 / Q000235:genetics* / Q000254:growth & development