Suppression of the cr-1 mutation in Neurospora crassa.
We have cloned a DNA fragment, which hybridized with the adenylate cyclase gene (CYR1) of Saccharomyces cerevisiae, from genomic DNA libraries of Neurospora crassa. The cr-1 mutation was able to be suppressed by introducing this DNA fragment on a cosmid vector, judging from recovery of the adenylate cyclase activity and the abnormal morphology.
Kore-eda
S
S
Institute of Applied Microbiology, University of Tokyo.
Murayama
T
T
Uno
I
I
D000262:Adenylyl Cyclases / Q000235:genetics*; D003001:Cloning, Molecular; D003360:Cosmids; D004247:DNA; D015723:Gene Library; D005822:Genetic Vectors; D006824:Hybridization, Genetic; D009154:Mutation; D009492:Neurospora crassa / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D013489:Suppression, Genetic
D004247:DNA; D000262:Adenylyl Cyclases