Temperature-sensitive mutations at the carboxy terminus of the alpha subunit of the Escherichia coli F1F0 ATP synthase.
Mutations were constructed in the a subunit of the F1F0 ATP synthase from Escherichia coli. Truncated forms of this subunit showed a temperature sensitivity phenotype. We conclude that the carboxy terminus of the a subunit is not involved directly with proton translocation but that it has an important structural role.
Vik
S B
SB
Department of Biological Sciences, Southern Methodist University, Dallas, Texas 75275-0376.
Lee
D
D
Marshall
P A
PA
D000595:Amino Acid Sequence; D001483:Base Sequence; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D006180:Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature
D046911:Macromolecular Substances; D006180:Proton-Translocating ATPases