Inhibition of c-mos protein kinase blocks mouse zygotes at the pronuclei stage.
The c-mos gene product is required for activation of the maturation-promoting factor (MPF) during oocyte maturation. The c-mos protein also acts as a cytostatic factor which is responsible for meiotic metaphase arrest of vertebrate eggs via stabilization of MPF. Here we show that mouse zygotes contain the c-mos protein. Introduction of a kinase-inhibitory anti-mos antibody into mouse zygotes 12 h after fertilization prevented the first cleavage of zygotes at the pronuclei stage. A second anti-mos antibody, known to allow the mos kinase to function, did not interfere with the formation of two-cell embryos. In addition to its known role in MPF activation in oocyte maturation and meiotic metaphase arrest, our findings indicate that the c-mos protein kinase is also required for completion of pronuclei breakdown following fertilization of mouse eggs.
Zhao
X
X
Department of Medicine, Stanford University School of Medicine, California 94305.
Singh
B
B
Arlinghaus
R B
RB
D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D005314:Embryonic and Fetal Development / Q000502:physiology*; D016200:Maturation-Promoting Factor / Q000502:physiology; D000090204:Mesothelin; D008677:Metaphase; D051379:Mice; D008969:Molecular Sequence Data; D009866:Oogenesis / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000502:physiology; D017231:Proto-Oncogene Proteins c-mos; D015053:Zygote / Q000166:cytology* / Q000378:metabolism
C000715528:Msln protein, mouse; D011518:Proto-Oncogene Proteins; D017231:Proto-Oncogene Proteins c-mos; D016200:Maturation-Promoting Factor; D000090204:Mesothelin