title: Cloning the mating types of the heterothallic fungus Podospora anserina: developmental features of haploid transformants carrying both mating types.
abstract: DNAs that encode the mating-type functions (mat+ and mat-) of the filamentous fungus Podospora anserina were cloned with the use of the mating-type A probe from Neurospora crassa. Cloning the full mat information was ascertained through gene replacement experiments. Molecular and functional analyses of haploid transformants carrying both mating types lead to several striking conclusions. Mat+ mat- strains are dual maters. However, the resident mat information is dominant to the mat information added by transformation with respect to fruiting body development and ascus production. Moreover, when dual mating mat+ mat- strains are crossed to mat+ or mat- testers, there is strong selection, after fertilization, that leads to the loss from the mat+ mat- nucleus of the mat information that matches that of the tester. Finally, the mat locus contains at least two domains, one sufficient for fertilization, the other necessary for sporulation.
authors.affiliation: Laboratoire de Génétique, URA 1354 du CNRS, Université Paris XI, Orsay, France.
authors.forename: M
authors.initials: M
authors.lastname: Picard
authors.forename: R
authors.initials: R
authors.lastname: Debuchy
authors.forename: E
authors.initials: E
authors.lastname: Coppin
mesh_terms: D001203:Ascomycota / Q000235:genetics*; D003001:Cloning, Molecular; D015342:DNA Probes / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D049770:Genes, Mating Type, Fungal; D000072235:Mating Factor; D009492:Neurospora crassa / Q000235:genetics; D010455:Peptides / Q000235:genetics*; D012641:Selection, Genetic; D013172:Spores, Fungal / Q000235:genetics; D014170:Transformation, Genetic / Q000235:genetics
chemical_list: D015342:DNA Probes; D010455:Peptides; D000072235:Mating Factor