title: Monoclonal antibody to the receptor for murine coronavirus MHV-A59 inhibits viral replication in vivo.
abstract: Because many strains of mouse hepatitis virus (MHV) infect laboratory mice, no effective vaccine has yet been developed. An alternative approach to control MHV disease is the use of a host cell receptor-targeted ligand. To address the potential usefulness of this approach, a monoclonal antibody directed against the host cell receptor for the coronavirus MHV-A59 was administered to infant mice that were then challenged oronasally with 10(4) intracerebral infant mouse median lethal doses of MHV-A59. Antibody treatment of virus-challenged mice resulted in lower proportions of mice with MHV-A59 in target organs and markedly reduced viral titers in these organs compared with mock-treated infected mice. Some antibody-treated infected mice survived for 7 days after viral challenge, whereas no mock-treated, infected mice survived beyond day 3 after viral inoculation. These results support a receptor-targeted approach to intervention in coronavirus disease.
authors.affiliation: Section of Comparative Medicine, Yale University School of Medicine, New Haven, CT 06510.
authors.forename: A L
authors.initials: AL
authors.lastname: Smith
authors.forename: C B
authors.initials: CB
authors.lastname: Cardellichio
authors.forename: D F
authors.initials: DF
authors.lastname: Winograd
authors.forename: M S
authors.initials: MS
authors.lastname: de Souza
authors.forename: S W
authors.initials: SW
authors.lastname: Barthold
authors.forename: K V
authors.initials: KV
authors.lastname: Holmes
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001921:Brain / Q000382:microbiology; D006524:Hepatitis, Viral, Animal / Q000276:immunology* / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D051379:Mice; D006517:Murine hepatitis virus / Q000276:immunology* / Q000502:physiology; D011991:Receptors, Virus / Q000276:immunology*; D014779:Virus Replication / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D011991:Receptors, Virus