A novel approach to insertional mutagenesis of Haemophilus influenzae.
Insertional mutagenesis of the Haemophilus influenzae chromosome was accomplished by a novel method employing a 2.2-kbp element, TSTE. This element, consisting of the neo gene of Tn5 flanked by Haemophilus-specific uptake sequences, was ligated to circularized chromosomal fragments before transformation into the homologous strain. Eight mutants defective in the production of haemocin were detected. This strategy provides an efficient mechanism for the insertional mutagenesis of H. influenzae.
Sharetzsky
C
C
Department of Pediatrics, Medical College of Pennsylvania, Philadelphia 19129.
Edlind
T D
TD
LiPuma
J J
JJ
Stull
T L
TL
D004251:DNA Transposable Elements; D006193:Haemophilus influenzae / Q000235:genetics*; D019868:Kanamycin Kinase; D016254:Mutagenesis, Insertional; D009693:Nucleic Acid Hybridization; D010770:Phosphotransferases / Q000378:metabolism; D012271:Ribostamycin / Q000494:pharmacology; D014169:Transformation, Bacterial
D004251:DNA Transposable Elements; D012271:Ribostamycin; D010770:Phosphotransferases; D019868:Kanamycin Kinase