title: Pyrophosphate formation as the most efficient condensation reaction of activated nucleotides.
abstract: When an oligonucleotide primer pG10 is incubated with the nucleotide analogue 9-[3-hydroxy-2-(hydroxymethyl)prop-1-yl] guanine diphosphate (pGp, I) in the presence of poly(C), addition of the monomer occurs almost exclusively at the 5'-terminal phosphate rather than the 3'-terminal cis-glycol. The implications of this finding in the context of prebiotic condensation reactions are discussed.
authors.affiliation: Salk Institute for Biological Studies, San Diego, CA 92122.
authors.forename: L
authors.initials: L
authors.lastname: Rodriguez
authors.forename: L E
authors.initials: LE
authors.lastname: Orgel
mesh_terms: D002851:Chromatography, High Pressure Liquid; D011756:Diphosphates / Q000737:chemistry*; D006150:Guanine Nucleotides / Q000737:chemistry*; D011066:Poly C / Q000737:chemistry*; D013698:Templates, Genetic
chemical_list: D011756:Diphosphates; D006150:Guanine Nucleotides; D011066:Poly C