title: Phagocytosis of beta-1,3-D-glucan-derivatized microbeads by mouse peritoneal macrophages involves three different receptors.
abstract: Intraperitoneal injection of beta-1,3-D-glucan coupled to the surface of monodisperse methacrylate microbeads improves the resistance against bacterial infections in mice, while methacrylate microbeads alone do not. The effect of the glucan-derivatized microbeads (GDM) is considered to be mediated through peritoneal macrophages. We show that both GDM and the underivatized methacrylate microbeads (UDM) treated with normal serum were rapidly bound and phagocytized by mouse peritoneal macrophages in vitro. We found that both complement and fibronectin opsonized the beads and were responsible for the uptake. Treatment of microbeads with serum lacking fibronectin and complement activity still gave some uptake of GDM, but not uptake of UDM. The uptake of GDM was similar to the uptake of untreated GDM and was inhibited by pretreatment of macrophages with soluble beta-1,3-D-glucan. Our conclusion is that GDM and UDM intraperitoneally bind fibronectin and C3 through activation of the alternative pathway of complement. This leads to their phagocytosis by macrophages through fibronectin and complement receptors. GDM are also internalized via beta-glucan receptors. We present the hypothesis that the beta-glucan receptors on peritoneal macrophages account for the protective effect of GDM in intraperitoneal bacterial infections.
authors.affiliation: Institute of Medical Biology, University of Tromsø, Norway.
authors.forename: Z
authors.initials: Z
authors.lastname: Konopski
authors.forename: L T
authors.initials: LT
authors.lastname: Rasmussen
authors.forename: R
authors.initials: R
authors.lastname: Seljelid
authors.forename: T
authors.initials: T
authors.lastname: Eskeland
mesh_terms: D000818:Animals; D005260:Female; D005936:Glucans / Q000276:immunology* / Q000378:metabolism; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D008863:Microspheres; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis; D011956:Receptors, Cell Surface / Q000276:immunology* / Q000378:metabolism; D011951:Receptors, Complement / Q000276:immunology* / Q000378:metabolism; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000276:immunology* / Q000378:metabolism; D047071:beta-Glucans
chemical_list: D005936:Glucans; D011956:Receptors, Cell Surface; D011951:Receptors, Complement; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic; D047071:beta-Glucans; C045268:beta-glucan receptor; C033363:beta-1,3-glucan