Human immunodeficiency virus type 1 vif, vpr, and vpu mutants can produce persistently infected cells.
A series of human immunodeficiency virus type 1 (HIV-1) mutants in vif, vpr, vpu, and nef were constructed from an infectious plasmid (pNL 432) containing the full-length HIV-1 DNA by frameshift mutations. The capacities for replication and cell killing of these mutant viruses were examined in a clonal cell line (M 10) isolated from HTLV-I-transformed MT-4 cells. In all cases, the mutant viruses replicated, expressed HIV-1 antigens, and induced drastic cytopathic effects. However, some M 10 cells survived infection with vif, vpr, and vpu mutant viruses and became persistently HIV-1-infected, whereas no cells survived infection with the nef mutant as well as the wild-type virus. The HIV-1 particles produced from the surviving cells after infection with the vif, vpr, or vpu mutant viruses were fully replicative in M 10 cells without apparent cytopathic effects.
Nishino
Y
Y
Institute of Immunological Science, Hokkaido University, Japan.
Kishi
M
M
Sumiya
M
M
Ogawa
K
K
Adachi
A
A
Maotani-Imai
K
K
Kato
S
S
Hirai
K
K
Ikuta
K
K
D016923:Cell Death; D002999:Clone Cells; D003588:Cytopathogenic Effect, Viral; D016368:Frameshift Mutation; D015754:Genes, nef; D016341:Genes, vif; D016334:Genes, vpr; D016338:Genes, vpu; D015497:HIV-1 / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D014779:Virus Replication