Fluctuation of the microbiota of the tongue in humans.
The purpose of this study was to examine whether variations occur in the % of motile micro-organisms on the dorsum of the tongue during a 1-month time-period and further to examine 2 transport fluids: the growth medium BM and reduced transport fluid (RTF), for their ability to conserve motility of microorganisms in comparison to sterile saline. Both transport fluids showed a significant reduction in the % of motile micro-organisms within 15 min. The use of RTF resulted, during a 60-min period, in a significantly higher % of motile micro-organisms compared to sterile saline. Results showed that the % of motile micro-organisms on the dorsum of the tongue was not constant. A significant reduction in the % of motile micro-organisms was observed in the course of the month involved in this study.
van der Weijden
G A
GA
Department of Periodontology, Academic Centre for Dentistry Amsterdam, The Netherlands.
van der Velden
U
U
D000704:Analysis of Variance; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D018407:Bacterial Physiological Phenomena; D001431:Bacteriological Techniques; D002364:Caseins; D002465:Cell Movement / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D006801:Humans; D008858:Microscopy, Phase-Contrast; D010100:Oxygen / Q000494:pharmacology; D011492:Protein Hydrolysates / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013144:Spirochaetales / Q000302:isolation & purification / Q000502:physiology; D013997:Time Factors; D014059:Tongue / Q000382:microbiology*
D002364:Caseins; D003470:Culture Media; D011492:Protein Hydrolysates; C026375:trypticase-soy broth; D012965:Sodium Chloride; D010100:Oxygen