title: Developmental genetic studies of the moss, Physcomitrella patens.
abstract: The development of the haploid gametophyte stage of Physcomitrella patens presents excellent opportunities for the detailed study of plant morphogenesis at the cellular level. The filamentous protonema undergoes a number of developmental transitions that can be observed directly in living material using time-lapse video microscopy and that can be manipulated both by treatment with phytohormones and by environmental stimuli. Mutants affecting these processes can be isolated and can be analysed using conventional as well as parasexual methods. Molecular biological techniques are now being established since these will be essential if the mechanisms that bring about morphogenetic processes are to be understood at the molecular level. A technique for genetic transformation has been devised and is being exploited to attempt to tag developmentally-relevant genes using maize transposons. Changes in gene activity associated with developmental transitions and in response to treatment with phytohormones and environmental stimuli are being studied using cDNA library subtraction techniques. Heterologous genes involved in the control of transcription and of the cell cycle are being used to probe the P. patens genome to identify possible homologues involved in developmental regulation.
authors.affiliation: Department of Genetics, University of Leeds, UK.
authors.forename: D J
authors.initials: DJ
authors.lastname: Cove
authors.forename: W
authors.initials: W
authors.lastname: Kammerer
authors.forename: C D
authors.initials: CD
authors.lastname: Knight
authors.forename: M J
authors.initials: MJ
authors.lastname: Leech
authors.forename: C R
authors.initials: CR
authors.lastname: Martin
authors.forename: T L
authors.initials: TL
authors.lastname: Wang
mesh_terms: D003583:Cytokinins / Q000502:physiology; D005786:Gene Expression Regulation; D017343:Genes, Plant; D005821:Genetic Techniques; D006112:Gravitation; D007210:Indoleacetic Acids / Q000502:physiology; D008027:Light; D009154:Mutation; D063245:Plant Development; D010944:Plants / Q000235:genetics
chemical_list: D003583:Cytokinins; D007210:Indoleacetic Acids