Effects of PP-56 and vitamin E on platelet hyperaggregability, fatty acid abnormalities, and clinical manifestations in streptozocin-induced diabetic rats.
The effects of vitamin E and D-myo-inositol 1,2,6-trisphosphate (PP-56) were investigated in long-term studies in streptozocin-induced diabetic rats fed a purified diet with 33% lipids and a polyunsaturated-to -saturated fatty acid ratio of 1. A supplement of vitamin E decreased plasma triglycerides, platelet lipid biosynthesis, some of the delta 6- and delta 5-desaturase abnormalities, and urine ketone bodies but did not affect the response of platelets to aggregation. PP-56 completely normalized the platelet reactivity to ADP and thrombin. This was accompanied by normalization of platelet lipid biosynthesis and diabetes-induced abnormalities in delta 6- and delta 5-desaturases. PP-56 treatment also reduced the mortality rate and to a certain extent urinary ketone bodies. The protective effect of PP-56 on platelet aggregation and mortality rate were dose related. PP-56, a molecule derived from phytic acid, seems to exert potent protective effects on some of the manifestations associated with diabetes in rats.
Ruf
J C
JC
National Institute of Health and Medical Research, Lyon-Bron, France.
Ciavatti
M
M
Gustafsson
T
T
Renaud
S
S
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000378:metabolism*; D007295:Inositol Phosphates / Q000494:pharmacology; D008055:Lipids / Q000097:blood; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D013311:Streptozocin; D013917:Thrombin / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology*
D005227:Fatty Acids; D007295:Inositol Phosphates; D008055:Lipids; D014810:Vitamin E; D013311:Streptozocin; D000244:Adenosine Diphosphate; D013917:Thrombin; D002118:Calcium; C059368:atrinositol