title: The C. elegans unc-104 gene encodes a putative kinesin heavy chain-like protein.
abstract: Mutations in the unc-104 gene of the nematode C. elegans result in uncoordinated and slow movement. Transposon insertions in three unc-104 alleles (e2184, rh1016, and rh1017) were used as physical markers to clone the unc-104 gene. DNA sequence analysis of unc-104 cDNAs revealed an open reading frame capable of encoding a 1584 amino acid protein with similarities to kinesin heavy chain. The similarities are greatest in the amino-terminal ATPase and microtubule-binding domains. Although the primary sequence relatedness to kinesin is weak in the remainder of the molecule, the predicted secondary structure and regional isoelectric points are similar to kinesin heavy chain.
authors.affiliation: Department of Biological Sciences, Illinois State University, Normal 61761-6901.
authors.forename: A J
authors.initials: AJ
authors.lastname: Otsuka
authors.forename: A
authors.initials: A
authors.lastname: Jeyaprakash
authors.forename: J
authors.initials: J
authors.lastname: García-Añoveros
authors.forename: L Z
authors.initials: LZ
authors.lastname: Tang
authors.forename: G
authors.initials: G
authors.lastname: Fisk
authors.forename: T
authors.initials: T
authors.lastname: Hartshorne
authors.forename: R
authors.initials: R
authors.lastname: Franco
authors.forename: T
authors.initials: T
authors.lastname: Born
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002107:Caenorhabditis / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D015246:Deoxyribonuclease EcoRI; D016547:Kinesins; D008870:Microtubules / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D015183:Restriction Mapping
chemical_list: D004251:DNA Transposable Elements; D000255:Adenosine Triphosphate; D004247:DNA; D015246:Deoxyribonuclease EcoRI; D000251:Adenosine Triphosphatases; D016547:Kinesins