Effects of chloroquine on lysosomes and endocytosis by liver cells in vivo.
1. Chloroquine accumulation in rat liver after a single and repeated drug administration and lysosomal changes resembling some symptoms of lysosomal storage diseases were observed. 2. Repeated chloroquine treatment of rats resulted in increased activity of liver lysosomal enzymes acid phosphatase and beta-galactosidase and a significant enhancement of the activities of cathepsin D and cysteine proteinases were found. 3. No changes in the activity of liver macrophages (as assessed by the colloidal carbon clearance test) or in fluid-phase endocytosis of the marker 125I-polyvinyl-pyrrolidone by hepatocytes in vivo were found.
Korolenko
T A
TA
Laboratory of Cellular Biochemistry and Physiology, Siberian Branch of the Academy of Medical Sciences of USSR, Novosibirsk.
Rukavishnikova
E V
EV
Safina
A F
AF
Mynkina
G I
GI
Vereshchagin
E I
EI
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002403:Cathepsins / Q000378:metabolism; D002738:Chloroquine / Q000494:pharmacology*; D003546:Cysteine Endopeptidases / Q000378:metabolism; D004705:Endocytosis / Q000502:physiology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D008247:Lysosomes / Q000378:metabolism*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D001616:beta-Galactosidase / Q000378:metabolism
D002738:Chloroquine; D000135:Acid Phosphatase; D001616:beta-Galactosidase; D002403:Cathepsins; D003546:Cysteine Endopeptidases