title: Trigonal crystals of porin from Escherichia coli.
abstract: Trigonal crystals of the integral membrane protein porin from Escherichia coli have been grown and characterized. They belong to space group P321 with unit cell constants a = b = LL8.4, c = 52.7 A, alpha = beta = 90 degrees, gamma = 120 degrees. The crystals grow as well-defined hexagonal prisms to a size of 0.25 mm in all dimensions, and diffract to 2.7 A. The molecular symmetry coincides with 3-fold crystallographic symmetry, giving two trimers per unit cell (1 monomer/asymmetric unit). This corresponds to VM = 2.9 A3/Da. Native X-ray data to 3.0 A resolution have been collected on a FAST area detector and a search for heavy atom derivatives is underway.
authors.affiliation: Department of Microbiology, University of Basel, Switzerland.
authors.forename: R A
authors.initials: RA
authors.lastname: Pauptit
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: G
authors.initials: G
authors.lastname: Rummel
authors.forename: T
authors.initials: T
authors.lastname: Schirmer
authors.forename: J N
authors.initials: JN
authors.lastname: Jansonius
authors.forename: J P
authors.initials: JP
authors.lastname: Rosenbusch
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D003460:Crystallization; D004926:Escherichia coli / Q000032:analysis*; D018272:Porins
chemical_list: D001425:Bacterial Outer Membrane Proteins; D018272:Porins