title: Production of interleukin-4 and other cytokines following stimulation of mast cell lines and in vivo mast cells/basophils.
abstract: Interleukin-3 (IL-3)-dependent mast cell lines, upon stimulation by calcium ionophores or by Fc epsilon RI cross-linking, express mRNA for, and secrete, a distinct pattern of cytokines, similar to those secreted by cloned mouse T cells of the TH2 type. The mast-cell-derived cytokines include IL-3, IL-4, IL-5 and IL-6. Not only in vitro mast cell lines, but also in vivo derived peritoneal mast cells secrete cytokines. An in vivo derived cell, in mouse spleen and bone marrow, secretes IL-4 and other cytokines upon stimulation with calcium ionophores or by Fc epsilon RI cross-linking or Fc gamma RII cross-linking. The IL-4-producing cells are highly enriched in the Fc epsilon R+ subset of spleen and bone marrow cells. These Fc epsilon R+ cells produce large amounts of IL-4, and they have characteristics similar to those of immature mast cells and/or basophils. It is possible that cytokines produced by mast cells and/or basophils participate in allergic inflammatory diseases.
authors.affiliation: Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Md.
authors.forename: R A
authors.initials: RA
authors.lastname: Seder
authors.forename: W E
authors.initials: WE
authors.lastname: Paul
authors.forename: S Z
authors.initials: SZ
authors.lastname: Ben-Sasson
authors.forename: G S
authors.initials: GS
authors.lastname: LeGros
authors.forename: A
authors.initials: A
authors.lastname: Kagey-Sobotka
authors.forename: F D
authors.initials: FD
authors.lastname: Finkelman
authors.forename: J H
authors.initials: JH
authors.lastname: Pierce
authors.forename: M
authors.initials: M
authors.lastname: Plaut
mesh_terms: D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D001491:Basophils / Q000187:drug effects / Q000378:metabolism*; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011961:Receptors, Fc / Q000032:analysis; D017455:Receptors, IgE
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D016207:Cytokines; D011961:Receptors, Fc; D017455:Receptors, IgE; D015847:Interleukin-4