Induction of anti-Theileria sergenti antibodies in calves with murine monoclonal anti-idiotype antibody.
Anti-idiotype (anti-Id) antibody that contains an internal image of Theileria sergenti (T. sergenti) merozoite surface antigen was intramuscularly inoculated to calves to induce anti-T. sergenti antibody. In immunized calves, anti-anti-Id antibodies were induced and these antibodies bound to merozoites of T. sergenti were confirmed by immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). In this experiment, one calf with the highest antibody titer against anti-Id antibody was intravenously challenged with T. sergenti-infected erythrocytes to show insufficient protective effects of the anti-anti-Id antibodies against the challenge.
Tanaka
M
M
Division of Veterinary Microbiology, Kyoto Biken Laboratories, Japan.
Ikeda
M
M
Matsuba
T
T
Okabe
T
T
Takahashi
K
K
Onuma
M
M
Sasaki
N
N
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D008262:Macrophage Activation; D016794:Theileria / Q000276:immunology*; D013801:Theileriasis / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary
D000888:Antibodies, Anti-Idiotypic; D000913:Antibodies, Protozoan