Modulation by thiols of the merocyanine 540-sensitized photolysis of leukemia cells, red cells, and herpes simplex virus type 1.
This paper reports on the role of endogenous and exogenous thiols in the merocyanine 540 (MC 540)-sensitized photoirradiation of L1210 leukemia cells, human erythrocytes, and human Herpes simplex virus type 1. Several measures taken to decrease the intracellular content of glutathione enhanced the cells' sensitivity to MC 540-sensitized photoirradiation while stimulation of glutathione biosynthesis or supplementation of the extracellular or extraviral thiol content decreased the photosensitivity of cells and viruses. Taken together, these data suggest that endogenous and exogenous thiols can modulate the sensitivity of cells and enveloped viruses to MC 540-sensitized photoirradiation. They also pose new questions as to the mechanism of MC 540-sensitized photolysis.
Gaffney
D K
DK
Department of Biochemistry, Medical College of Wisconsin, Milwaukee 53226.
O'Brien
J M
JM
Sieber
F
F
D000818:Animals; D002460:Cell Line; D004912:Erythrocytes / Q000187:drug effects* / Q000528:radiation effects; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007939:Leukemia L1210 / Q000378:metabolism; D008027:Light; D051379:Mice; D011744:Pyrimidinones / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D018139:Simplexvirus / Q000187:drug effects* / Q000528:radiation effects; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
D011744:Pyrimidinones; D011838:Radiation-Sensitizing Agents; D013438:Sulfhydryl Compounds; C003954:merocyanine dye; D005978:Glutathione