[Cloning and sequencing of a highly repeated DNA fragment of rabbit].
We obtained highly repeated DNA fragments from a rabbit by using restriction endonuclease HindIII digestion of chromosomal DNA; the smallest highly repeated DNA fragment was cloned into vector plasmid pUC12. We used the recombinant plasmid to transform E. coli JM101, selected and identified the positive clone by DNA probe in situ hybridization as well as Southern blotting. We obtained a recombinant clone [termed pRAb (Hind III)-1] which contains the highly repeated DNA fragment [termed RAb (Hind III)-1]. The complete nucleotide sequence of the 345bp RAb (Hind III)-1 fragment was determined by the dideoxynucleotide termination sequencing method. We analyzed the characteristics of the structure of the sequence with a microcomputer, and the results suggested that the RAb (Hind III)-1 sequence is quite different from alphoid DNA of primates.
Ding
J
J
Institute of Basic Medical Science, Beijing.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D008969:Molecular Sequence Data; D010957:Plasmids; D011817:Rabbits / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D013045:Species Specificity
D004274:DNA, Recombinant; D004247:DNA