title: Direct comparison of in-vitro and in-vivo Photofrin-II mediated photosensitization using a pulsed KTP pumped dye laser and a continuous wave argon ion pumped dye laser.
abstract: A pulsed KTP pumped dye laser (25 kHz repetition rate and 470 nsec pulse width) has been compared to a continuous wave argon ion pumped dye laser as the source of 630 nm light during in-vitro and in-vivo Photofrin-II mediated photosensitization studies. Individual experiments documented the effectiveness of each laser system on a) photosensitizer induced cytotoxicity and induction of stress protein synthesis using Chinese hamster fibroblasts; b) photobleaching of Photofrin-II in aqueous solution; c) Photofrin II mediated photosensitization of normal mouse skin; d) Photofrin II mediated photodynamic therapy of a mouse mammary carcinoma; and e) tumor temperature levels generated during laser exposure. Comparable results were obtained for both laser systems in all experiments.
authors.affiliation: Clayton Ocular Oncology Center, Childrens Hospital of Los Angeles, CA 90027.
authors.forename: A
authors.initials: A
authors.lastname: Ferrario
authors.forename: N
authors.initials: N
authors.lastname: Rucker
authors.forename: S W
authors.initials: SW
authors.lastname: Ryter
authors.forename: D R
authors.initials: DR
authors.lastname: Doiron
authors.forename: C J
authors.initials: CJ
authors.lastname: Gomer
mesh_terms: D000818:Animals; D001128:Argon; D002277:Carcinoma / Q000188:drug therapy; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D017323:Dihematoporphyrin Ether; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D006415:Hematoporphyrins / Q000008:administration & dosage / Q000627:therapeutic use*; D053685:Laser Therapy; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D010710:Phosphates; D010778:Photochemotherapy / Q000295:instrumentation / Q000379:methods*; D010787:Photosensitivity Disorders / Q000139:chemically induced; D012878:Skin Neoplasms / Q000188:drug therapy; D013053:Spectrophotometry; D013696:Temperature; D014025:Titanium
chemical_list: D006360:Heat-Shock Proteins; D006415:Hematoporphyrins; D010710:Phosphates; C064806:potassium titanylphosphate; D001128:Argon; D017323:Dihematoporphyrin Ether; D014025:Titanium