title: Evaluation of the intraperitoneal route for the transfusion of erythrocytes using rats and dogs.
abstract: The intraperitoneal transfusion of red cells in rats and dogs was evaluated as an alternative route for blood restoration. Erythrocytes labeled with 59Fe, equivalent to about 50% of the estimated total red cell volume, were injected in the abdominal cavity of 5 dogs. Three other dogs and 12 rats were injected with a volume proportional to the 9%. The maximal incorporation of tagged red cells in the circulation of the rats was of 96 +/- 7% at 48 h. One half of the erythrocytes disappeared from the circulation 29 days later. Computing data from all dogs, the mean highest value, 91.2 +/- 6%, was reached at 96 h and although random destruction of the cells occurred, the estimated T1/2 of survival was about 66 days. The injection was completed in about 15 seconds in the rats and in 2 to 4 minutes in the dogs. At the necropsy only minor serosal reactions were observed in the peritoneal cavity of the animals. Intraperitoneal transfusion can be recommended as a rapid, safe and efficient procedure for blood supply.
authors.affiliation: Departamento de Fisiopatología, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Prov. de Buenos Aires, Argentina.
authors.forename: M A
authors.initials: MA
authors.lastname: Aba
authors.forename: A A
authors.initials: AA
authors.lastname: Pissani
authors.forename: R H
authors.initials: RH
authors.lastname: Alzola
authors.forename: I
authors.initials: I
authors.lastname: Videla Dorna
authors.forename: M D
authors.initials: MD
authors.lastname: Ghezzi
authors.forename: N A
authors.initials: NA
authors.lastname: Marcilese
mesh_terms: D000818:Animals; D016913:Blood Component Transfusion / Q000379:methods*; D004285:Dogs; D004905:Erythrocyte Aging; D004906:Erythrocyte Count; D004911:Erythrocyte Volume; D004912:Erythrocytes / Q000000981:diagnostic imaging*; D005069:Evaluation Studies as Topic; D007274:Injections, Intraperitoneal; D007504:Iron Radioisotopes; D011877:Radionuclide Imaging; D051381:Rats
chemical_list: D007504:Iron Radioisotopes