Evaluation of somatic cell variants deficient in glycosylphosphatidyl-inositol anchoring as candidates for genetic correction.
Many cell surface glycoproteins are anchored in the lipid bilayer by a glycosylphosphatidyl-inositol (GPI) structure. Recently, a number of cell lines which are deficient in the biosynthesis and/or addition of this anchor have been described. In this report, we summarize the current knowledge on these lines and discuss their potential use to isolate the genes involved in the GPI anchor biosynthetic pathway with a specific emphasis on L cell fibroblasts.
Fasel
N
N
Institute of Biochemistry, University of Lausanne, Epalines, Switzerland.
Deglon
N
N
Rousseaux
M
M
Beghdadi-Rais
C
C
Bernard
M
M
Bron
C
C
D000818:Animals; D002460:Cell Line; D006017:Glycolipids / Q000096:biosynthesis* / Q000235:genetics; D017261:Glycosylphosphatidylinositols; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured
D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D010716:Phosphatidylinositols