Identification of cellular factors that bind specifically to the Epstein-Barr virus origin of DNA replication.
The specific binding of HeLa cell factors to DNA sequences at the Epstein-Barr virus (EBV) latent origin of DNA replication was detected by gel shift experiments and DNase I footprinting analysis. These cellular proteins protected at least five discrete regions of the DNA replication origin. The viral protein required for EBV plasmid replication, EBV nuclear antigen 1 (EBNA-1), binds to specific sequences within the origin region. The HeLa cell proteins competed with EBNA-1 for binding to EBV origin DNA in vitro, leading to the possibility that these cellular proteins regulate EBV DNA replication by displacing EBNA-1 at the origin sites.
Oh
S J
SJ
Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, New Jersey 08544-1014.
Chittenden
T
T
Levine
A J
AJ
D000956:Antigens, Viral; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D003850:Deoxyribonuclease I; D019309:Epstein-Barr Virus Nuclear Antigens; D006367:HeLa Cells / Q000378:metabolism; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D009710:Nucleotide Mapping; D015345:Oligonucleotide Probes; D010957:Plasmids; D011485:Protein Binding
D000956:Antigens, Viral; D004279:DNA, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D009687:Nuclear Proteins; D015345:Oligonucleotide Probes; D003850:Deoxyribonuclease I