Detection of bluetongue virus by using bovine endothelial cells and embryonated chicken eggs.
Two systems, inoculation of bovine endothelial cells and of embryonated chicken eggs, were compared for detection of bluetongue virus (BTV) in blood specimens from experimentally inoculated sheep. For all BTV serotypes tested, embryonated chicken eggs detected longer periods of viremia than did bovine endothelial cells, primarily by detecting BTV in samples containing lower virus concentrations.
Wechsler
S J
SJ
Arthropod-borne Animal Diseases Research Laboratory, U.S. Department of Agriculture, Laramie, Wyoming 82071-3965.
Luedke
A J
AJ
D000818:Animals; D001819:Bluetongue / Q000150:complications / Q000175:diagnosis*; D001820:Bluetongue virus / Q000302:isolation & purification*; D002417:Cattle; D002460:Cell Line; D002642:Chick Embryo / Q000382:microbiology; D004730:Endothelium, Vascular / Q000382:microbiology; D012756:Sheep; D014766:Viremia / Q000150:complications; D014773:Virology / Q000379:methods*