title: Altered polymorphonuclear leukocyte Fc gamma R expression contributes to decreased candicidal activity during intraabdominal sepsis.
abstract: We investigated the effects of untreated intraabdominal sepsis on polymorphonuclear leukocyte (PMN) candicidal activity. Two groups of swine were studied. Group I (n = 6) underwent sham laparotomy, group II (n = 7) underwent cecal ligation and incision. Untreated intraabdominal sepsis resulted in a progressive decrease in PMN candicidal activity. Concomitant rosetting and phagocytosis assays demonstrated a decrease in both the attachment and phagocytosis of Candida albicans opsonized with both normal and septic swine serum by PMNs in group II. Iodine 125-labeled swine immunoglobulin G (IgG) and fluorescein isothioalanate (FITC)-labeled swine IgG were used to investigate Fc gamma receptor ligand interactions. Scatchard analyses demonstrated a progressive decline in both the binding affinity constant and number of IgG molecules bound per PMN. Stimulation of the oxidative burst markedly reduced 125I-labeled IgG binding in both group I and group II, with a greater decrement being seen in animals with intraabdominal sepsis. Further, in group II, PMN recycling of the Fc gamma receptor to the cell surface after generation of the oxidative burst was reduced by postoperative day 4. Binding of monoclonal antibodies to Fc gamma receptor II, but not Fc gamma receptor I/III markedly reduced intracellular candicidal activity. Immunofluorescence studies revealed a homogeneous pattern of FITC-IgG uptake by nearly all group I PMNs, whereas by postoperative day 8 a substantial number of PMNs from group II failed to internalize the FITC-IgG. These studies suggest that untreated intraabdominal sepsis reduces PMN candicidal activity and that this is due, in part, to altered PMN Fc gamma receptor ligand interactions.
authors.affiliation: Department of Surgery, Rhode Island Hospital, Providence 02903.
authors.forename: H H
authors.initials: HH
authors.lastname: Simms
authors.forename: R
authors.initials: R
authors.lastname: D'Amico
authors.forename: P
authors.initials: P
authors.lastname: Monfils
authors.forename: K W
authors.initials: KW
authors.lastname: Burchard
mesh_terms: D000005:Abdomen / Q000382:microbiology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000943:Antigens, Differentiation / Q000378:metabolism / Q000502:physiology*; D002176:Candida albicans / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D002177:Candidiasis / Q000382:microbiology / Q000503:physiopathology*; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007457:Iodine Radioisotopes; D009504:Neutrophils / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010586:Phagocytes / Q000502:physiology; D011961:Receptors, Fc / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D017452:Receptors, IgG; D013552:Swine / Q000382:microbiology*; D013861:Thiocyanates
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D005452:Fluoresceins; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D011961:Receptors, Fc; D017452:Receptors, IgG; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate