Periprosthetic chronic inflammation characterized through the measurement of superoxide anion production by synovial-derived macrophages.
Periprosthetic macrophages were isolated from the synovium of primary and revision arthroplasty patients. Inflammatory activity was determined by the level of superoxide (O2-) production de novo and in response to phorbol myristate acetate (PMA) stimulation. Nonstimulated primary arthroplasty-derived macrophages produced 2.54 +/- 2.04 pmoles of O2-/minute/10(5) cells. When identical reaction tubes were stimulated with PMA, O2- levels increased to 5.76 +/- 3.77 pmol of O2-/minute/10(5) cells. Nonstimulated revision arthroplasty-derived macrophages produced 3.26 +/- 2.02 pmol of O2-/minute/10(5) cells during this ten-minute time period. When identical reaction tubes were stimulated with PMA, O2- levels increased to 3.98 +/- 2.52 pmol of O2-/minute/10(5) cells. The difference in the ratio of O2- production in response to stimulation between primary and revision groups was statistically significant. The observation of a chronic moderate level of activation and the lack of responsiveness to a potent stimulator suggests that macrophage inflammatory activity is down-regulated in periprosthetic synovium.
Kossovsky
N
N
Department of Pathology and Laboratory Medicine, UCLA Medical Center 90024-1732.
Liao
K
K
Millett
D
D
Feng
D
D
Campbell
P A
PA
Amstutz
H C
HC
Finerman
G A
GA
Thomas
B J
BJ
Kilgus
D J
DJ
Cracchiolo
A
A
D000328:Adult; D000368:Aged; D001862:Bone Resorption / Q000276:immunology; D006622:Hip Prosthesis; D006801:Humans; D007249:Inflammation / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008875:Middle Aged; D011475:Prosthesis Failure; D012086:Reoperation; D013481:Superoxides / Q000737:chemistry*; D013582:Synovial Fluid / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D013481:Superoxides; D013755:Tetradecanoylphorbol Acetate