T, not B, lymphocytes are required for immunodeficiency and paralysis induced by ts1, a mutant of Moloney murine leukemia virus-TB.
BALB/c mice when injected as newborn with ts1, a temperature-sensitive mutant of Moloney murine leukemia virus-TB, developed a fatal hindlimb paralysis and immunodeficiency. This disease induction was prevented, to a great extent, by transient depletion of the peripheral T lymphocytes during the early course of infection by using anti-Thy 1.2 antibody. FVB/N mice, which are highly susceptible to ts1, but express Thy 1.1 instead of Thy 1.2 on their T lymphocytes, did not show any difference in the disease profile when treated similarly with anti-Thy 1.2 antibody. Transient depletion of the peripheral B lymphocytes in BALB/c mice in the early course of ts1 infection had no effect on the disease induction. In the T cell depleted BALB/c mice, virus replication was reduced, survival of the mice was increased and viral specific antibodies were produced, whereas, in the B cell depleted mice the disease process went on in a fashion similar to untreated mice infected with ts1. Thus, this study demonstrates that the disease syndrome induced by ts1 in BALB/c mice is dependent upon the presence of T lymphocytes during the early course of infection, and that presence of B lymphocytes have little or no effect on the disease outcome.
Saha
K
K
Department of Carcinogenesis, University of Texas, M.D. Anderson Cancer Center, Smithville 78957.
Wong
P K
PK
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D051379:Mice; D008807:Mice, Inbred BALB C; D008979:Moloney murine leukemia virus / Q000235:genetics*; D016183:Murine Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000382:microbiology; D009154:Mutation; D013601:T-Lymphocytes / Q000276:immunology*; D014779:Virus Replication / Q000235:genetics