Activation and cytotoxicity of 5'-deoxy-5-fluorouridine in c-H-ras transformed NIH 3T3 cells.
Transformation of NIH 3T3 cells with c-H-ras has been demonstrated to result in significantly increased activation of 5'-deoxy-5-fluorouridine and significantly increased cytotoxicity in vitro as compared to non-transformed NIH 3T3. FUra cytotoxicity appeared to be increased also in vitro upon transformation; the level of significance however was beyond that of accepted significance (0.05 less than P less than 0.01). Furthermore dFUrd proved to be less active in vivo in nude mice bearing v-fos transformed NIH 3T3 cells than in nude mice bearing c-H-ras transformed cells.
Geng
Y
Y
Laboratory of Cancer Research & Clinical Oncology, University of Antwerp, Belgium.
Gheuens
E
E
de Bruijn
E A
EA
D000818:Animals; D001711:Biotransformation; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005467:Floxuridine / Q000494:pharmacology*; D005472:Fluorouracil / Q000378:metabolism; D011905:Genes, ras / Q000187:drug effects*; D051379:Mice; D008819:Mice, Nude; D013329:Structure-Activity Relationship; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000187:drug effects
D005467:Floxuridine; D005472:Fluorouracil; C025034:doxifluridine