[Indirect immunofluorescence with monoclonal antibodies in the diagnosis of Pneumocystis carinii. Preliminary report].
We compared traditional methods based on argentic metamine and toluidine blue staining vs recently introduce techniques based on immunofluorescence for monoclonal antibodies in the diagnosis of P. carinii. Fifty samples were analyzed: 29 (58%) were negative by all method. Of the remaining 21.72% were positive for immunofluorescence, 67% for argentic and 48% for toluidine blue staining. Thus, immunofluorescence techniques represent an improvement in the ability to diagnose infection by P carinii.
Weitz
J C
JC
Laboratorio de Referencia de Parasitología, Instituto de Salud Pública, Santiago de Chile.
Astorga
B
B
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan / Q000032:analysis* / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D011020:Pneumonia, Pneumocystis / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan
Inmunofluorescencia indirecta con anticuerpos monoclonales en el diagnóstico de Pneumocystis carinii. Informe preliminar.