Sleep promoting effect of a putative glial gamma-aminobutyric acid uptake blocker applied in the thalamus of cats.
The uptake of gamma-aminobutyric acid (GABA) by glial cells was decreased when 4,5,6,7,-tetrahydroisoxazolo-(4,5-C)-pyridin-3-ol (THPO) was applied in the thalamus of freely moving cats by in vivo microdialysis. A marked reduction in duration of wakefulness and in number of awakenings was obtained during THPO treatment. THPO did not change the ratio of slow-wave-sleep and paradoxical sleep but only increased the total sleep time. The present data suggest a possible regulatory role of the glial-neuronal interaction in the modification of the sleep-waking cycle.
Juhász
G
G
Department of Comparative Physiology, Eötvös Loránd University, Budapest, Hungary.
Kékesi
K A
KA
Emri
Z
Z
Ujszászi
J
J
Krogsgaard-Larsen
P
P
Schousboe
A
A
D000818:Animals; D002415:Cats; D003956:Dialysis; D005110:Extracellular Space / Q000378:metabolism; D007555:Isoxazoles / Q000494:pharmacology*; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D014179:Neurotransmitter Uptake Inhibitors / Q000494:pharmacology*; D010477:Perfusion; D012890:Sleep / Q000187:drug effects*; D013788:Thalamus / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D014851:Wakefulness / Q000187:drug effects; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D007555:Isoxazoles; D014179:Neurotransmitter Uptake Inhibitors; C026804:4,5,6,7-tetrahydroisoxazolo(4,5-c)pyridin-3-ol; D005680:gamma-Aminobutyric Acid