Pseudomonas aeruginosa toxins: effect on Ehrlich carcinoma and Vero cells in vitro.
The main objective of the present investigation was to screen P. aeruginosa toxins for in vitro activity and investigate biochemical mode of action. Toxins were isolated after concentration of the culture filtrate and separation of the filtrate on Sephadex G-100 column in four fractions. On the basis of primary screening results the dialysate and fractions 3 and 4 effectively inhibited incorporation of [14C]adenine (DNA, RNA) and [14C]valine (protein) into the TCA-insoluble fraction of both Ehrlich ascites carcinoma (EAC) and Vero cells. The toxins also interfered with energy-yielding processes (endogenous respiration). The dialysate decreased the level of ATP in EAC cells after two hours incubation in vitro.
Majtan
V
V
Institute of Preventive and Clinical Medicine, Bratislava, Czechoslovakia.
Miko
M
M
Hostacka
A
A
D036002:ADP Ribose Transferases; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism* / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects*; D004734:Energy Metabolism / Q000187:drug effects; D005098:Exotoxins / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D010101:Oxygen Consumption / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D014709:Vero Cells; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D000255:Adenosine Triphosphate; D001427:Bacterial Toxins; D005098:Exotoxins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A