[Phosphoglucomutase subtyping by means of agarose thin-layer electrophoresis. A study on applying different buffer systems and agaroses].
For the routine subtyping of PGM1 isozymes by means of agarose gel thin-layer electrophoresis, a tris-maleic acid-buffer and an acetic acid-buffer, several agaroses with electroendosmosis up to -0, 16, and several papers are tested. With all these materials good results can be obtained, so that agarose without electroendosmosis is not necessary and FN 1 papers can be used.
Hering
S
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Institut für gerichtliche Medizin, Medizinischen Akademie, Carl Gustav Carus Dresden.
D002021:Buffers; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification*; D010733:Phosphoglucomutase / Q000145:classification / Q000302:isolation & purification*; D012685:Sepharose
D002021:Buffers; D007527:Isoenzymes; D012685:Sepharose; D010733:Phosphoglucomutase
Phosphoglucomutase-Subtypisierung mittels Agarose-Dünnschichtelektrophorese. Studie zum Einstaz verschiedener Puffersysteme und Agarosen.