title: Activation of the cAMP cascade by steroidogenic hormones and glucagon in the pathogenic fungus Candida albicans.
abstract: Incubation of Candida albicans yeast cells with human luteinizing hormone (hLH), human chorionic gonadotrophin (hCG) or glucagon produced a significant rise in cAMP total levels. The effect of these hormones in permeabilized cells of the fungus produced a 2-3 fold increase in the Mg2+, GTP-dependent adenylyl cyclase activity as well as full activation of the cAMP-dependent protein kinase (PKA) activity. These results indicate that the interaction of the mammalian hormones with the fungus triggered the cAMP activation cascade in a similar way to that found in higher eukaryotic organisms.
authors.affiliation: Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
authors.forename: C
authors.initials: C
authors.lastname: Paveto
authors.forename: G
authors.initials: G
authors.lastname: Mallo
authors.forename: G
authors.initials: G
authors.lastname: Egidy
authors.forename: M A
authors.initials: MA
authors.lastname: Galvagno
authors.forename: S
authors.initials: S
authors.lastname: Passeron
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D002176:Candida albicans / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D005934:Glucagon / Q000494:pharmacology*; D006801:Humans; D007986:Luteinizing Hormone / Q000494:pharmacology*; D008274:Magnesium / Q000032:analysis / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D015290:Second Messenger Systems; D014050:Toluene / Q000494:pharmacology
chemical_list: D006063:Chorionic Gonadotropin; D014050:Toluene; D007986:Luteinizing Hormone; D005934:Glucagon; D000242:Cyclic AMP; D011494:Protein Kinases; D000262:Adenylyl Cyclases; D008274:Magnesium