The octapeptide corresponding to the region of the highest homology between alpha-interferon and thymosin-alpha 1 effectively competes with both cytokines for common high-affinity receptors on murine thymocytes.
The octapeptide corresponding to human interferon-alpha 2 (Hu IFN-alpha 2) sequence 131-138 has high affinity to murine thymocyte receptors (Kd = 4.2 x 10(-12) M, about 700 receptors per cell). The peptide receptor binding is inhibited by both Hu rIFN-alpha 2 (Ki = 8.6 x 10(-10) M) and thymosin-alpha 1 (TM-alpha 1) (Ki = 3 x 10(-7) M) as well as by the octapeptide homologous to the TM-alpha 1 sequence 16-23 (Ki = 4.5 x 10(-7) M). The peptide from IFN-alpha 2 (131-138) activates murine thymocyte blast transformation at a concentration of 10(-11) M in the presence of 2.5 micrograms/ml of concanavalin A.
Zav'yalov
V P
VP
Institute of Immunology, Moscow Region, Chekhov District, USSR.
Navolotskaya
E V
EV
Abramov
V M
VM
Galaktionov
V G
VG
Isaev
I S
IS
Kaurov
O A
OA
Kozhich
A T
AT
Maiorov
V A
VA
Prusakov
A N
AN
Vasilenko
R N
RN
D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000378:metabolism*; D008213:Lymphocyte Activation; D051379:Mice; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011994:Recombinant Proteins; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives* / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism*
D007370:Interferon Type I; D009842:Oligopeptides; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D013947:Thymosin; D000077596:Thymalfasin