title: Flow cytometric determination of breast tumor heterogeneity.
abstract: Flow cytometric analysis was done on the DNA content of nuclei obtained from different sites of small breast tumors. Although specimens for analysis were obtained within a few millimeters of each other, dramatic differences were occasionally observed in the DNA histograms. In a limited study involving 141 consecutive breast specimens submitted for flow cytometry, 52% (74) were found to have at least one DNA aneuploid population. In 18% of DNA aneuploid tumors, one or more specimens from areas grossly identified as tumor had no DNA aneuploid population. Because of the proposed correlation of aneuploidy with a poorer prognosis and possible responsiveness to chemotherapy, multiple sites should be assayed when flow cytometric DNA analysis is done.
authors.affiliation: Department of Medical Biology, University of Tennessee Medical Center, Knoxville 37920.
authors.forename: J E
authors.initials: JE
authors.lastname: Fuhr
authors.forename: A
authors.initials: A
authors.lastname: Frye
authors.forename: A A
authors.initials: AA
authors.lastname: Kattine
authors.forename: S
authors.initials: S
authors.lastname: Van Meter
mesh_terms: D000782:Aneuploidy; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results
chemical_list: D004273:DNA, Neoplasm