Mechanism of inhibition of HSV-1 replication by tumor necrosis factor and interferon gamma.
Tumor necrosis factor (TNF) synergizes with interferon (IFN gamma) in the blockade of HSV-1 replication. Antibodies against IFN beta block this synergism, implying a role of IFN beta in the antiviral activity of TNF plus IFN gamma. IFN beta 1 added exogenously to Hep-2 cells shows antiviral activity against HSV-1 only at high concentrations, whereas IFN beta 2 (also known as IL-6) alone has no effect on the replication of VSV or HSV-1 even when 1,000 U/ml are present. Our results are in accordance with the idea that TNF induces IFN beta 1 and that both cytokines must be present in the culture medium to synergize with IFN gamma in order to inhibit HSV-1 replication.
Feduchi
E
E
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
Carrasco
L
L
D000818:Animals; D000906:Antibodies; D002460:Cell Line; D004347:Drug Interactions; D004357:Drug Synergism; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology*; D008715:Methionine / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D018139:Simplexvirus / Q000187:drug effects / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects*
D000906:Antibodies; D007370:Interferon Type I; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D008715:Methionine