Isolation of Chinese hamster ovary cell lines producing Man3GlcNAc2 asparagine-linked glycans.
Chinese hamster ovary lines with two mutations, one causing accumulation of Man5GlcNAc2-P-P-dolichol and a second resulting in defective N-acetylglucosaminyltransferase I activity, synthesize asparagine-linked glycans with the structure Man3GlcNAc2. As a result, the asparagine-linked glycans produced by these lines are smaller and less heterogeneous than those produced by other currently available animal cell lines.
Zeng
Y
Y
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235.
Lehrman
M A
MA
D000818:Animals; D002240:Carbohydrate Sequence; D002460:Cell Line / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D003412:Cricetulus; D005838:Genotype; D005964:Glucosyltransferases / Q000172:deficiency; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases; D011104:Polyisoprenyl Phosphate Oligosaccharides / Q000302:isolation & purification / Q000378:metabolism*; D011134:Polysaccharides / Q000096:biosynthesis* / Q000302:isolation & purification
D011104:Polyisoprenyl Phosphate Oligosaccharides; D011134:Polysaccharides; C070437:mannosyl(3)-N-acetylglucosamine(2) diphosphate dolichol; D005964:Glucosyltransferases; D017351:N-Acetylglucosaminyltransferases; C024850:alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I