Interstitial chromosomal deletion within 4q11-q13 in a human hepatoma cell line.
Southern blot analysis revealed the presence of an aberrant albumin gene as well as a normal one in a human hepatoma cell line, HuH-7. A genomic sequence carrying this altered gene was isolated and characterized. This clone contains a 3-kbp 3' segment of the albumin gene linked to a non-albumin sequence at intron 11. The non-albumin sequence is assigned to chromosome 4q12-q13 by in situ hybridization. This indicates an interstitial deletion of a chromosomal segment within 4q11-q13 because the albumin gene is mapped there. The truncated albumin gene is detected in an early passage of HuH-7 cells and has been maintained stably in cell culture.
Urano
Y
Y
Department of Medical Biochemistry, University of Calgary, Alberta, Canada.
Watanabe
K
K
Lin
C C
CC
Hino
O
O
Tamaoki
T
T
D000418:Albumins / Q000235:genetics; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002872:Chromosome Deletion; D002894:Chromosomes, Human, Pair 4; D015321:Gene Rearrangement; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D014407:Tumor Cells, Cultured
D000418:Albumins