Isolation and characterization of lamprey rhodopsin cDNA.
Genomic DNA fragments coding a visual pigment of the lamprey were amplified by polymerase chain reaction, using oligonucleotide mixtures as primers. The complete coding region of the cDNA was obtained by separate amplification of both cDNA ends. The deduced amino acid sequence of the coding region showed 78-82% identity with those of rhodopsins of higher vertebrates, but only 43-47% identity with those of human color pigments. The cloned DNA appears to be the cDNA of a lamprey rhodopsin, which is expressed in the "short" photoreceptor cell.
Hisatomi
O
O
Department of Physics, Faculty of Science, Tohoku University, Sendai, Japan.
Iwasa
T
T
Tokunaga
F
F
Yasui
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D007798:Lampreys; D008099:Liver / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D015183:Restriction Mapping; D012243:Rhodopsin / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D015345:Oligonucleotide Probes; D004247:DNA; D012243:Rhodopsin