[The temperature dependence of protein kinase A and C activity in variant mouse neuroblastoma cells differing genetically in their heat resistance].
The ability of the mouse neuroblastoma cell line NTR to proliferate at 40 degrees C correlates with the position of the temperature optimum of protein kinases A and C activities in the region of higher temperatures compared to those for cells of the original line N18AI, and with higher thermostability of protein kinase A after its heating at various elevated temperatures. The found changes in protein kinases A and C in the cells of NTR line mean that the selection of variants, capable of growing at elevated temperatures, is accompanied with conformational protein changes.
Konstantinova
M F
MF
Nekrasova
T P
TP
Nisman
B Kh
BKh
D000818:Animals; D002460:Cell Line; D004795:Enzyme Stability; D014644:Genetic Variation / Q000502:physiology*; D006358:Hot Temperature; D051379:Mice; D009447:Neuroblastoma / Q000201:enzymology* / Q000235:genetics; D011493:Protein Kinase C / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D013696:Temperature; D014407:Tumor Cells, Cultured / Q000201:enzymology
D011494:Protein Kinases; D011493:Protein Kinase C
Temperaturnaia zavisimost' aktivnosti proteinkinaz A i C u variantov kletok neĭroblastomy myshi, geneticheski razlichaiushchikhsia po teploustoĭchivosti.