Proliferation patterns of latent Pneumocystis carinii in rat organs during progressive stages of immunosuppression.
Pneumocystis carinii is known to proliferate mainly in the lung of an immunocompromised host. In AIDS and other immune disorders sporadic extrapulmonary presence of this organism has been documented. Occasionally, P. carinii does not appear to infect the lung. These observations have been based on the detection of P. carinii by conventional staining techniques. We have sought to determine the extent of these infections by the polymerase chain reaction (PCR) in a rat model. Harlan Sprague-Dawley rats weighing between 110 and 130 g were immunosuppressed with dexamethasone (1.2 mg/l) in drinking water. During progressive stages of immunosuppression 2 rats were sacrificed at 2, 3, 4 and 5 wk, and the lung, liver, kidney, spleen and bone marrow were taken. Sonicated crude extracts of the tissues were used as template DNA for the amplification of the dihydrofolate reductase (DHFR) gene of P. carinii. All the PCR products were analyzed by Southern hybridization with radiolabelled DHFR DNA. These analyses revealed a general trend of P. carinii proliferation first in bone marrow at 2 wk, followed by liver at 3 wk, and lung at 5 wk on immunosuppression. Kidney and spleen infections were infrequent. Although P. carinii appears to proliferate in the lung at later stages of immunosuppression, the degree of proliferation is several-fold greater than in extrapulmonary organs. The extrapulmonary proliferation of P. carinii, however small, may possibly suppress hematopoietic stem cell differentiation in bone marrow, and may also contribute to the pathology present in various organs.
Reddy
L V
LV
Center for Molecular Biology, MCHT, Wayne State University, Detroit, MI 48201.
Zammit
C
C
D000818:Animals; D001483:Base Sequence; D001853:Bone Marrow / Q000469:parasitology; D003907:Dexamethasone; D004195:Disease Models, Animal; D005260:Female; D007165:Immunosuppression Therapy; D008099:Liver / Q000469:parasitology; D008168:Lung / Q000469:parasitology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011010:Pneumocystis / Q000254:growth & development* / Q000276:immunology; D016720:Pneumocystis Infections / Q000276:immunology / Q000469:parasitology; D016133:Polymerase Chain Reaction; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000469:parasitology
D003907:Dexamethasone