Generation of adriamycin radical by interaction of adriamycin with serum.
ESR signal of the adriamycin (AD) free radical was obtained when adriamycin interacted with serum under anaerobic conditions, but it was not observed by the interaction of AD with erythrocytes or the lysate. The intensities of the ESR signals were dependent upon the concentrations of adriamycin and serum, and cytochrome c completely diminished the ESR signal. Addition of reduced pyridine nucleotides stimulated the formation of AD radical. Between pH 5.0 and 8.0, more of AD radical was formed at higher pH. Allopurinol and arsenite, which are flavin enzyme inhibitors, partially inhibited the AD radical formation by serum. From these results, it seems that several flavin enzymes in serum are involved in the AD radical formation.
Miura
T
T
Hokkaido Institute of Pharmaceutical Sciences, Otaru, Japan.
Muraoka
S
S
Ogiso
T
T
D000693:Anaerobiosis; D000818:Animals; D003574:Cytochrome c Group / Q000494:pharmacology; D004317:Doxorubicin / Q000097:blood* / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D005415:Flavins / Q000097:blood; D005609:Free Radicals; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D009249:NADP / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D003574:Cytochrome c Group; D005415:Flavins; D005609:Free Radicals; D009249:NADP; D004317:Doxorubicin