title: Comparison of albumin receptors expressed on bovine and human group G streptococci.
abstract: The albumin receptor expressed by bovine group G streptococci was extracted and affinity purified. The protein was characterized for species reactivity, and monospecific antibodies were prepared to the purified receptor. The bovine group G albumin receptor was compared functionally, antigenically, and for DNA homology with the albumin-binding protein expressed by human group G streptococci. In agreement with previous reports, the albumin-binding activity of human strains was mediated by a unique domain of the type III immunoglobulin G-Fc-binding molecule, protein G. The albumin receptor expressed by bovine group G strains was found to lack any immunoglobulin G-binding potential but displayed a wider profile of species albumin reactivity than protein G. Both albumin receptors could inhibit the binding of the other to immobilized human serum albumin, and each displayed similar binding properties. Antigenic comparison of the two albumin receptors demonstrated a low level of cross-reactivity; however comparison at the DNA level, using an oligonucleotide probe specific for the albumin-binding region of protein G, demonstrated that the two albumin receptors expressed by human and bovine group G streptococcal strains do not display significant homology.
authors.affiliation: Department of Microbiology, Medical College of Ohio, Toledo 43699-0008.
authors.forename: R
authors.initials: R
authors.lastname: Raeder
authors.forename: R A
authors.initials: RA
authors.lastname: Otten
authors.forename: M D
authors.initials: MD
authors.lastname: Boyle
mesh_terms: D000418:Albumins / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D004247:DNA / Q000032:analysis; D006801:Humans; D008970:Molecular Weight; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D013291:Streptococcus / Q000032:analysis*
chemical_list: D000418:Albumins; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface; D004247:DNA