Interaction of 5-bromowillardiine with non-NMDA receptors expressed in Xenopus laevis oocytes injected with chick brain mRNA.
Excitatory amino acid (EAA) receptors were studied in Xenopus laevis oocytes microinjected with 1-day-old chick brain mRNA using a two-electrode voltage clamp technique. EAA agonists could be differentially characterised according to their current/voltage (I/V) relationships; I/V plots to kainate (KA) and domoate (Dom) inwardly rectified at positive membrane potentials whereas quisqualate (QA), alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and L-glutamate (L-Glu) produced linear I/V plots. Interestingly, the non-NMDA (N-methyl-D-aspartate) agonist, 5-bromowillardiine (BrW), displayed properties similar to KA and Dom. It is proposed that BrW acts as a KA-like agonist on chick EAA receptors expressed in Xenopus oocytes.
Bowie
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Department of Pharmacology, School of Pharmacy, London, U.K.
Smart
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D000409:Alanine / Q000031:analogs & derivatives*; D000818:Animals; D001921:Brain; D002645:Chickens; D005260:Female; D007051:Ibotenic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D007267:Injections; D007608:Kainic Acid / Q000031:analogs & derivatives; D008564:Membrane Potentials / Q000187:drug effects; D009467:Neuromuscular Depolarizing Agents; D009865:Oocytes / Q000378:metabolism*; D016318:Quisqualic Acid; D012333:RNA, Messenger / Q000008:administration & dosage / Q000378:metabolism*; D011954:Receptors, Dopamine / Q000187:drug effects*; D018092:Kainic Acid Receptors; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology; D017981:Receptors, Neurotransmitter / Q000378:metabolism; D014982:Xenopus laevis / Q000378:metabolism*; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
D000409:Alanine; D007051:Ibotenic Acid; D007608:Kainic Acid; D009467:Neuromuscular Depolarizing Agents; D016318:Quisqualic Acid; D012333:RNA, Messenger; D011954:Receptors, Dopamine; D018092:Kainic Acid Receptors; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; C068592:5-bromowillardiine; C012301:domoic acid