title: Purification and characterization of a thymocyte growth factor. 2. Biological activity of the thymocyte growth factor.
abstract: A thymocyte growth factor, THGF, purified from the supernatant of a thymic T-lymphocyte-precursor cell line (TC.SC-1/2.0), stimulated the growth of splenocytes and thymocytes that had not been activated by a mitogen. Addition of suboptimal doses of mitogen or phorbol myristate acetate did not enhance the response. The growth factor could stimulate the growth of thymocytes synergistically with interleukin 2 but its action was not mediated by interleukin 2 or by interleukin 4. L3T4-Lyt2- thymocytes, Ig- splenocytes, Ig+ splenocytes, and alpha 1-thymosin-sensitive splenocytes were targets for THGF, which also stimulated the formation of haemopoietic spleen colonies in irradiated mice.
authors.affiliation: Research Institute of Epidemiology and Microbiology, Ministry of Health of Russia, Nizhnii Novgorod.
authors.lastname: Talaev VYu
authors.forename: A A
authors.initials: AA
authors.lastname: Yarilin
authors.forename: N I
authors.initials: NI
authors.lastname: Sharova
authors.forename: V P
authors.initials: VP
authors.lastname: Shichkin
authors.forename: T M
authors.initials: TM
authors.lastname: Shuvaeva
mesh_terms: D000818:Animals; D002455:Cell Division; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D006133:Growth Substances / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008808:Mice, Inbred CBA; D010455:Peptides / Q000502:physiology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000737:chemistry* / Q000166:cytology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013947:Thymosin / Q000502:physiology
chemical_list: D003470:Culture Media; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C052275:thymocyte growth peptide; D003208:Concanavalin A; D013947:Thymosin; D013755:Tetradecanoylphorbol Acetate