Stimulation of atrial natriuretic peptide secretion and synthesis by Na-K-ATPase inhibitors.
Ouabain has been reported to increase the secretion of ANP in vitro. In this study, we focused on whether this action is common in Na-K-ATPase inhibitors (ATPI) and whether ATPI simply increase the release of ANP or stimulate both its biosynthesis and release. The effects of ouabain and digoxin on secretion of ANP and accumulation of ANP mRNA were investigated in the rat cardiocyte superfusion system. Ouabain and digoxin increased the immunoreactive ANP (iANP) output into perfusate and accumulation of ANP mRNA significantly. These results suggest that ATPI may stimulate both ANP biosynthesis and release in vitro.
Morise
T
T
Second Department of Internal Medicine, School of Medicine, Kanazawa University, Japan.
Takeuchi
Y
Y
Okamoto
S
S
Takeda
R
R
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics* / Q000378:metabolism; D004077:Digoxin / Q000494:pharmacology; D006321:Heart / Q000187:drug effects; D008297:Male; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
D012333:RNA, Messenger; D010042:Ouabain; D004077:Digoxin; D009320:Atrial Natriuretic Factor; D000254:Sodium-Potassium-Exchanging ATPase