In vitro susceptibility of Trichophyton mentagrophytes arthroconidia to clotrimazole and griseofulvin in human corneocyte suspensions.
The use of corneocytes in suspension as a medium for the study of the effect of antifungal drugs on Trichophyton mentagrophytes arthroconidia was investigated. In the presence of clotrimazole or griseofulvin, arthroconidia germination in a suspension of corneocytes and in Sabouraud glucose liquid medium was significantly reduced compared with germination in drug-free media. Where antifungals were added to arthroconidia after an activation period during which germination occurred, any further germination was inhibited. The data showed that the growth of T. mentagrophytes in the presence of corneocytes offered a simple, rapid, inexpensive and relevant model for the assessment of antifungal activity of compounds for the treatment of dermatophytosis.
Aljabre
S H
SH
Department of Dermatology, University of Glasgow, UK.
Scott
E M
EM
Shankland
G S
GS
Richardson
M D
MD
D003022:Clotrimazole / Q000494:pharmacology*; D000078404:Epidermal Cells; D004817:Epidermis / Q000382:microbiology; D006118:Griseofulvin / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D008954:Models, Biological; D014249:Trichophyton / Q000187:drug effects*
D006118:Griseofulvin; D003022:Clotrimazole