New approaches towards automated oligoribonucleotide synthesis.
Several protecting group combinations have been investigated to achieve an automated oligoribonucleotide synthesis on a solid support. The use of 2'-O-tert-butyldimethylsilyl-5'-O-monomethoxytrityl-ribonucleoside -3'-(p-nitrophenylethyl, N,N-diethyl)-phosphoramidites in conjunction with beta-eliminating blocking groups at the aglycone gave satisfactory results. An inverse protecting group strategy based upon analogously substituted 5'-O-2-dansylethoxycarbonyl-2'-O-4- methoxytetrahydropyran-4-yl-phosphoramidites can be regarded as another promising approach.
Pfleiderer
W
W
Fakultät für Chemie, Universität Konstanz, FRG.
Stengele
K P
KP
Mohr
M
M
Bergmann
F
F
D001483:Base Sequence; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D012265:Ribonucleotides
D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D012265:Ribonucleotides