Analysis of the patterns of expression of mRNAs for the alpha- and beta-subunits of the lysosomal enzyme beta-N-acetylhexosaminidase in mouse epididymis and testis.
mRNA for the alpha- and beta-subunits of mouse beta-N-acetylhexosaminidase were detected in tissue sections of epididymis and testis using 3H-labelled antisense RNA probes by hybridization in situ. There were clear differences in the intensity of the signals given by the two tissues with alpha-subunit mRNA being more abundant in testis than epididymis, while the opposite was so with beta-subunit mRNA. Differences in the isoenzyme patterns of the two tissues are likely to be due, at least in part, to differences in transcription by the Hexa and Hexb genes.
Stirling
J L
JL
Division of Biomolecular Sciences, King's College London, Campden Hill, UK.
Beccari
T
T
Hoade
J
J
Pezzetti
F
F
Calvitti
M
M
Becchetti
E
E
Orlacchio
A
A
D000818:Animals; D004822:Epididymis / Q000201:enzymology*; D054818:Hexosaminidase A; D054819:Hexosaminidase B; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D013737:Testis / Q000201:enzymology*; D014316:Tritium; D001619:beta-N-Acetylhexosaminidases / Q000235:genetics*
D012333:RNA, Messenger; D014316:Tritium; D054818:Hexosaminidase A; D054819:Hexosaminidase B; D001619:beta-N-Acetylhexosaminidases