title: The human PIM-1 gene product is a protein serine kinase.
abstract: The human PIM-1 gene, a homologue of murine retroviral insertion site mpim-1, is overexpressed in a subset of hematolymphoid malignancies. Deduced amino acid sequence of PIM-1 complementary DNA predicts it to be a protein kinase. In vitro transcription coupled translation of the putative 313-amino acid open reading frame yields a Mr 34,000 protein; an immune complex kinase assay of the wild-type PIM-1 and not a site-directed mutant, in which the invariant Lys67 has been changed to Arg, demonstrates autophosphorylating activity on serine residues. Thus, PIM-1 is a protein serine kinase with a possible role in neoplastic transformation.
authors.affiliation: Department of Hematology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
authors.forename: R
authors.initials: R
authors.lastname: Padma
authors.forename: L
authors.initials: L
authors.lastname: Nagarajan
mesh_terms: D001483:Base Sequence; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins / Q000737:chemistry / Q000235:genetics*; D051573:Proto-Oncogene Proteins c-pim-1; D014158:Transcription, Genetic
chemical_list: D011518:Proto-Oncogene Proteins; D011494:Protein Kinases; C496634:PIM1 protein, human; D017346:Protein Serine-Threonine Kinases; D051573:Proto-Oncogene Proteins c-pim-1