Stress-induced change in cerebral ATP+ubiquitin-dependent proteinase activity vary with age.
Stress-induced changes in cerebral ATP+ubiquitin-dependent proteinase activity were studied and the effect of age on it was checked. For that purpose 23,000 g supernatant prepared from whole brain of 3- and 7-month-old rats after 6h long immobilization stress were used. With azocasein as substrate, at pH 8.0 values of ATP+ubiquitin-dependent proteinase activity increased for 20% and 10% in 3- and 7-month-old animals respectively. Following 24 h long immobilization, values of cerebral ATP + ubiquitin-dependent activity in 3-month-old animals dropped by 16%. Data obtained indicate that immobilization stress affects ATP+ubiquitin-dependent proteinase activity and point to the contribution of age in the modulation of enzyme response to stress.
Azaryan
A
A
Institute of Biochemistry, Yerevan, USSR.
Dovanjyan
G
G
Lajtha
A
A
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D010450:Endopeptidases / Q000378:metabolism*; D008297:Male; D009043:Motor Activity; D051381:Rats; D013312:Stress, Physiological / Q000378:metabolism*
C080306:ATP-ubiquitin-dependent proteinase; D010450:Endopeptidases