title: Demonstration of the genuine iso-12p character of the standard marker chromosome of testicular germ cell tumors and identification of further chromosome 12 aberrations by competitive in situ hybridization.
abstract: The recently developed competitive in situ hybridization (CISH) strategy was applied to the analysis of chromosome 12 aberrations in testicular germ cell tumors (TGCTs). DNAs from two rodent-human somatic cell hybrids, containing either a normal chromosome 12 or the p arm of chromosome 12 as their unique human material, were used as probes. Our results demonstrate a genuine iso-12p character of the standard marker chromosome in TGCTs. Moreover, variant markers were identified representing translocation products that also involve chromosome 12.
authors.affiliation: Department of Human Genetics, University Hospital Nijmegen, The Netherlands.
authors.forename: R F
authors.initials: RF
authors.lastname: Suijkerbuijk
authors.forename: A Y
authors.initials: AY
authors.lastname: van de Veen
authors.forename: J
authors.initials: J
authors.lastname: van Echten
authors.forename: C H
authors.initials: CH
authors.lastname: Buys
authors.forename: B
authors.initials: B
authors.lastname: de Jong
authors.forename: J W
authors.initials: JW
authors.lastname: Oosterhuis
authors.forename: D A
authors.initials: DA
authors.lastname: Warburton
authors.forename: J J
authors.initials: JJ
authors.lastname: Cassiman
authors.forename: D
authors.initials: D
authors.lastname: Schonk
authors.forename: A
authors.initials: A
authors.lastname: Geurts van Kessel
mesh_terms: D002869:Chromosome Aberrations; D002881:Chromosomes, Human, Pair 12; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D008297:Male; D009373:Neoplasms, Germ Cell and Embryonal / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D013736:Testicular Neoplasms / Q000235:genetics*; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D015342:DNA Probes; D004273:DNA, Neoplasm; D005819:Genetic Markers