Primary structure and relative potency of an analog of beta-PDH (pigment-dispersing hormone) from the crayfish Procambarus clarkii.
A pigment-dispersing hormone (PDH) from eyestalks of the crayfish Procambarus clarkii was purified by gel filtration, cation-exchange chromatography, partition chromatography, and reversed-phase HPLC. Based on automated sequencing and by the identical chromatographic behavior of the native PDH and the synthetic amidated form of the deduced sequence, the primary structure of Procambarus PDH has been established as: Asn-Ser-Glu-Leu-Ile-Asn-Ser-Ile-Leu-Gly-Leu-Pro-Lys-Val-Met-Asn-Glu-Ala- NH2. This peptide differs from beta-PDH of the fiddler crab Uca pugilator at a single position, Glu17 in place of Asp17. Because of this substitution, Procambarus PDH was 4 to 7-fold less potent than beta-PDH in causing pigment dispersion in the erythrophores, leucophores, and melanophores of Uca. In contrast, Procambarus PDH was 4-fold more potent than beta-PDH in eliciting pigment dispersion in the erythrophores of Procambarus. These peptides displayed less marked differences in potency in triggering leucophore pigment dispersion and light-adaptational distal eye pigment movement in Procambarus. These findings indicate that the structural requirements for PDH-receptor interactions vary with the species and with the target cell type within a given species.
McCallum
M L
ML
Department of Biology, University of West Florida, Pensacola 32514-5751.
Rao
K R
KR
Riehm
J P
JP
Mohrherr
C J
CJ
Morgan
W T
WT
D000595:Amino Acid Sequence; D000818:Animals; D003400:Astacoidea / Q000737:chemistry*; D003386:Brachyura; D005123:Eye / Q000737:chemistry; D007447:Invertebrate Hormones / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology*; D008547:Melanophores / Q000502:physiology; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology*; D012168:Retinal Pigments / Q000737:chemistry
D007447:Invertebrate Hormones; D010455:Peptides; D012168:Retinal Pigments; C027714:melanophore-dispersing hormone