title: A calcium-dependent protein kinase with a regulatory domain similar to calmodulin.
abstract: Calcium can function as a second messenger through stimulation of calcium-dependent protein kinases. A protein kinase that requires calcium but not calmodulin or phospholipids for activity has been purified from soybean. The kinase itself binds calcium with high affinity. A complementary DNA clone for this kinase has been identified; it encodes a protein with a predicted molecular mass of 57,175 daltons. This protein contains a catalytic domain similar to that of calmodulin-dependent kinases and a calmodulin-like region with four calcium binding domains (EF hands). The predicted structure of this kinase explains its direct regulation via calcium binding and establishes it as a prototype for a new family of calcium-regulated protein kinases.
authors.affiliation: Department of Botany, Louisiana State University, Baton Rouge 70803.
authors.forename: J F
authors.initials: JF
authors.lastname: Harper
authors.forename: M R
authors.initials: MR
authors.lastname: Sussman
authors.forename: G E
authors.initials: GE
authors.lastname: Schaller
authors.forename: C
authors.initials: C
authors.lastname: Putnam-Evans
authors.forename: H
authors.initials: H
authors.lastname: Charbonneau
authors.forename: A C
authors.initials: AC
authors.lastname: Harmon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D002118:Calcium / Q000378:metabolism / Q000502:physiology*; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002147:Calmodulin / Q000235:genetics*; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013025:Glycine max / Q000201:enzymology* / Q000235:genetics
chemical_list: D002118:Calcium; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002147:Calmodulin; D011494:Protein Kinases