Compatibility of organic solvents with the Microscreen prophage-induction assay: solvent--mutagen interactions.
The following solvents did not induce prophage lambda in the Escherichia coli WP2s(lambda) Microscreen assay: acetone, benzene, chloroform, ethanol, n-hexane, isopropanol, methanol, toluene, and a mixture of the three isomers of xylene. Dimethyl sulfoxide was genotoxic in the presence and absence of S9, and methylene chloride was weakly genotoxic in the presence of S9. The genotoxic potencies of 2-aminoanthracene and 2-nitrofluorene were reduced when dissolved in DMSO or methanol compared to their potencies when dissolved in acetone.
DeMarini
D M
DM
Genetic Toxicology Division, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711.
Lawrence
B K
BK
Brooks
H G
HG
Houk
V S
VS
D000873:Anthracenes / Q000633:toxicity; D010582:Bacteriophage lambda / Q000187:drug effects; D004249:DNA Damage; D004260:DNA Repair; D004121:Dimethyl Sulfoxide / Q000633:toxicity; D004926:Escherichia coli / Q000378:metabolism; D005449:Fluorenes / Q000633:toxicity; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens; D012997:Solvents; D010948:Viral Plaque Assay; D014775:Virus Activation / Q000187:drug effects*
D000873:Anthracenes; D005449:Fluorenes; D009153:Mutagens; D012997:Solvents; C019499:2-nitrofluorene; C006593:2-anthramine; D004121:Dimethyl Sulfoxide