title: Possible heterogeneity of metabotropic glutamate receptors induced in Xenopus oocytes by rat brain mRNA.
abstract: Pharmacological properties of metabotropic glutamate receptors were studied in Xenopus oocytes injected with rat brain mRNA. trans-1-Amino-cyclopentyl-1,3-dicarboxylic acid (t-ACPD), a conformationally restricted analog of glutamate, induced oscillatory inward currents in mRNA-injected oocytes. These t-ACPD responses showed several characteristics identical to those of the other metabotropic responses including the metabotropic glutamate responses stimulated by quisqualate. D,L-2-Amino-3-phophonopropionate (D,L-AP3) effectively suppressed the t-ACPD and ibotenate responses. However, quisqualate responses were not affected substantially by D,L-AP3. These findings suggest that the metabotropic glutamate receptors in the oocytes may be classified into at least two subtypes according to their pharmacological properties: one preferentially activated by quisqualate and insensitive to AP3, and the other activated by t-ACPD and ibotenate and antagonized by AP3.
authors.affiliation: Department of Biology, Faculty of Science, Okayama University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Tanabe
authors.forename: I
authors.initials: I
authors.lastname: Ito
authors.forename: H
authors.initials: H
authors.lastname: Sugiyama
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001923:Brain Chemistry; D003515:Cycloleucine / Q000031:analogs & derivatives / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000494:pharmacology; D007051:Ibotenic Acid / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism*; D016318:Quisqualic Acid / Q000494:pharmacology; D012333:RNA, Messenger / Q000494:pharmacology*; D051381:Rats; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000378:metabolism*; D014981:Xenopus / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; C000211:2-amino-3-phosphonopropionic acid; D003515:Cycloleucine; C036361:1-amino-1,3-dicarboxycyclopentane; D007051:Ibotenic Acid; D016318:Quisqualic Acid; D019204:GTP-Binding Proteins; D000409:Alanine