title: Isoniazid acetylating phenotype in patients with paracoccidioidomycosis and its relationship with serum sulfadoxin levels, glucose-6-phosphate dehydrogenase and glutathione reductase activities.
abstract: The authors evaluated the isoniazid acetylating phenotype and measured hematocrit, hemoglobin, glucose-6-phosphate dehydrogenase and glutathione reductase activities plus serum sulfadoxin levels in 39 patients with paracoccidioidomycosis (33 males and 6 females) aged 17 to 58 years. Twenty one (53.84%) of the patients presented a slow acetylating phenotype and 18(46.16%) a fast acetylating phenotype. Glucose-6-phosphate-dehydrogenase (G6PD) activity was decreased in 5(23.80%) slow acetylators and in 4(22.22%) fast acetylators. Glutathione reductase activity was decreased in 14(66.66%) slow acetylators and in 12(66.66%) fast acetylators. Serum levels of free and total sulfadoxin were higher in slow acetylator (p less than 0.02). Analysis of the results permitted us to conclude that serum sulfadoxin levels are related to the acetylator phenotype. Furthermore, sulfadoxin levels were always above 50 micrograms/ml, a value considered therapeutic. Glutathione reductase deficiency observed in 66% of patients may be related to the intestinal malabsorption of nutrients, among them riboflavin, a FAD precursor vitamin, in patients with paracoceidioidomycosis.
authors.affiliation: Department of Infectious and Parasitologic Diseases, School of Medicine of Botucatu, University Paulist State, São Paulo, Brazil.
authors.forename: B
authors.initials: B
authors.lastname: Barraviera
authors.forename: P C
authors.initials: PC
authors.lastname: Pereira
authors.forename: J M
authors.initials: JM
authors.lastname: Machado
authors.forename: M J
authors.initials: MJ
authors.lastname: de Souza
authors.forename: C R
authors.initials: CR
authors.lastname: Lima
authors.forename: P R
authors.initials: PR
authors.lastname: Curi
authors.forename: R P
authors.initials: RP
authors.lastname: Mendes
authors.forename: D A
authors.initials: DA
authors.lastname: Meira
mesh_terms: D000107:Acetylation; D000328:Adult; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D005980:Glutathione Reductase / Q000378:metabolism*; D006801:Humans; D007538:Isoniazid / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010229:Paracoccidioidomycosis / Q000097:blood / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D013413:Sulfadoxine / Q000097:blood*
chemical_list: D013413:Sulfadoxine; D005954:Glucosephosphate Dehydrogenase; D005980:Glutathione Reductase; D007538:Isoniazid