Induction of interleukin-1 beta mRNA in adult rat brain.
The ability of the central nervous system to produce the cytokine interleukin-1 beta (IL-1 beta) in response to challenge by activators of the mononuclear phagocyte system has been examined in vivo. Unilateral injection of a mixture of gamma-interferon (IFN-gamma) and lipopolysaccharide (LPS) into the forebrain of adult rats induced expression of IL-1 beta mRNA. In situ hybridization of IL-1 beta mRNA showed a gradient of cellular hybridization, which was most intense at the site of IFN-gamma/LPS injection. The reverse transcription--polymerase chain reaction (RT-PCR) was used to demonstrate the presence of IL-1 beta mRNA in normal rat brain, and to confirm increases in IL-1 beta mRNA levels following IFN-gamma/LPS injection. These studies show that IL-1 beta can be induced to high levels within the CNS as a consequence of exposure to potent stimulators of macrophage activation.
Higgins
G A
GA
Department of Neurobiology and Anatomy, University of Rochester Medical Center, NY 14642.
Olschowka
J A
JA
D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry / Q000187:drug effects*; D007371:Interferon-gamma / Q000494:pharmacology*; D007375:Interleukin-1 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical
D007375:Interleukin-1; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D007371:Interferon-gamma