T-cell receptor gene rearrangement analysis: cutaneous T cell lymphoma, peripheral T cell lymphoma, and premalignant and benign cutaneous lymphoproliferative disorders.
T-cell receptor gene rearrangement analysis is a useful technique to detect clonality and determine lineage of lymphoid neoplasms. We examined 103 patients with mycosis fungoides, Sézary syndrome, peripheral T cell lymphoma, potentially malignant lymphoproliferative disorders including pre-Sézary syndrome, large plaque parapsoriasis, lymphomatoid papulosis and follicular mucinosis, and various benign inflammatory infiltrates. A clonal rearrangement was detected in skin samples in 20 of 24 patients with mycosis fungoides and in peripheral blood samples in 19 of 21 patients with Sézary syndrome. A clonal population was also detected in seven of eight cases classified as peripheral T cell lymphoma. The potentially malignant dermatoses tended to have clonal rearrangement, with the exception of large plaque parapsoriasis, and further follow-up is needed to correlate clonality with the disease course. These studies demonstrate the value of molecular genetics as an adjunct to morphology in the examination of patients with cutaneous lymphoproliferative disease.
Zelickson
B D
BD
Department of Dermatology, Mayo Clinic, Rochester, MN 55905.
Peters
M S
MS
Muller
S A
SA
Thibodeau
S N
SN
Lust
J A
JA
Quam
L M
LM
Pittelkow
M R
MR
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D005260:Female; D015329:Gene Rearrangement, T-Lymphocyte / Q000235:genetics*; D006801:Humans; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000473:pathology; D016410:Lymphoma, T-Cell, Cutaneous / Q000097:blood / Q000235:genetics* / Q000473:pathology; D016411:Lymphoma, T-Cell, Peripheral / Q000097:blood / Q000235:genetics* / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000097:blood / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D000507:Mucinosis, Follicular / Q000097:blood / Q000235:genetics / Q000473:pathology; D009182:Mycosis Fungoides / Q000097:blood / Q000235:genetics / Q000473:pathology; D010267:Parapsoriasis / Q000097:blood / Q000235:genetics / Q000473:pathology; D011230:Precancerous Conditions / Q000097:blood / Q000235:genetics* / Q000473:pathology; D012751:Sezary Syndrome / Q000097:blood / Q000235:genetics / Q000473:pathology; D012867:Skin / Q000473:pathology; D012871:Skin Diseases / Q000097:blood / Q000235:genetics* / Q000473:pathology; D012878:Skin Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology
D004247:DNA