title: Long-lasting potentiation and epileptiform activity produced by GABAB receptor activation in the dentate gyrus of rat hippocampal slice.
abstract: Bath application of the GABAB receptor agonist baclofen produced a concentration-dependent long-lasting potentiation (LLP) of the evoked population spike in the dentate gyrus of rat hippocampal slices. A high concentration of baclofen (5 microM) also produced a loss of inhibition that was manifested as the appearance of epileptiform, multiple evoked population spikes and a decrease in paired-pulse inhibition. Both baclofen-induced potentiation and epileptiform activity could be blocked or significantly reduced in slices from pertussis toxin-treated animals (1 microgram, intradentate) or in slices pretreated with the NMDA receptor antagonist D-(-)-2-amino-5-phosphonovaleric acid (10 microM). At a concentration that had no significant effect on individual evoked responses (0.1 microM) but still produced a loss in paired-pulse inhibition, baclofen facilitated the induction of beta-adrenergic receptor-mediated LLP. LLP was induced in the dentate gyrus by bath application of 1 microM, but not 0.1 microM, isoproterenol. Coapplication of baclofen and isoproterenol, both at a concentration (0.1 microM) that individually had no effect on the population spike, produced a synergistic LLP of the population spike. We propose that baclofen produces a selective disinhibitory effect in the granule cell layer of the dentate gyrus by inhibiting the activity of GABAergic interneurons. At a low concentration, the subtle loss of inhibition can facilitate the induction of isoproterenol-induced LLP. At a high concentration, baclofen can produce an LLP that is probably induced by a loss of inhibition.
authors.affiliation: Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799.
authors.forename: E C
authors.initials: EC
authors.lastname: Burgard
authors.forename: J M
authors.initials: JM
authors.lastname: Sarvey
mesh_terms: D000818:Animals; D001418:Baclofen / Q000494:pharmacology*; D004558:Electric Stimulation; D004594:Electrophysiology / Q000379:methods; D004827:Epilepsy / Q000503:physiopathology*; D005071:Evoked Potentials / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D011712:Pyramidal Tracts / Q000187:drug effects / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
chemical_list: D011963:Receptors, GABA-A; D001418:Baclofen; D007545:Isoproterenol