title: Nonstructural protein NS2 of parvovirus H-1 is required for efficient viral protein synthesis and virus production in rat cells in vivo and in vitro.
abstract: We generated a mutation in the gene for the nonstructural protein NS2 of parvovirus H-1 in which the highly conserved dinucleotide AG at the 3' splice acceptor site of NS2 intron 1 was mutated to CG. The mutation does not change the amino acid sequence for NS1. The splice acceptor (SA) mutant gene was introduced into the H-1 virus (H-1SA) and an infectious clone of LuIII (pLuH1SA). The R2 transcripts encoding NS2 were absent by both Northern blot and primer extension analysis in the LuH1SA or H-1SA virus-infected cells and the NS2 protein was undetectable in the infected cell lysate by immunoprecipitation. These NS2 null mutant viruses were capable of lytic growth in cell lines that were derived from human, hamster, and dog, but they produced lower virus titers than wild-type H-1. The H-1SA virus nonproductively infected Rat2 rat fibroblasts and transformed Rat2 cell lines. Analysis of synchronized infections of rat fibroblasts demonstrated that H-1SA viral duplex replicative form DNA replication was reduced and that single-stranded progeny DNA was deficient compared to wild-type H-1. In addition, H-1SA viral protein synthesis was about 10% of wild-type virus and virions were not detectable in rat fibroblasts. However, H-1SA mRNAs R1 and R3 accumulated to wild-type levels. NS2 was also required for productive infection in newborn rats but not in newborn hamsters. These results indicate that NS2 plays an important role in the regulation of viral protein synthesis in rat cells in vivo and in vitro.
authors.affiliation: Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198.
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: S L
authors.initials: SL
authors.lastname: Rhode
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002213:Capsid / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics; D007700:Kinetics; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D015345:Oligonucleotide Probes; D010321:Parvoviridae / Q000235:genetics / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D051381:Rats; D015183:Restriction Mapping; D014758:Viral Core Proteins / Q000235:genetics / Q000378:metabolism*; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins / Q000096:biosynthesis*; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D015345:Oligonucleotide Probes; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins