The zinc finger protein GLI transforms primary cells in cooperation with adenovirus E1A.
The GLI gene was previously isolated by virtue of its amplification in human glioblastomas. We have now found that GLI expression can result in the in vitro transformation of both primary and secondary rodent cells. When coexpressed with adenovirus E1A, the GLI protein functions analogously to RAS, resulting in the formation of dense foci of cells which are tumorigenic in nude mice.
Ruppert
J M
JM
Johns Hopkins Oncology Center, Baltimore, Maryland 21231-1001.
Vogelstein
B
B
Kinzler
K W
KW
D017456:Adenovirus Early Proteins; D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004268:DNA-Binding Proteins / Q000502:physiology*; D005910:Glioma; D066298:In Vitro Techniques; D005810:Multigene Family; D009687:Nuclear Proteins / Q000502:physiology*; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D051381:Rats; D014157:Transcription Factors / Q000502:physiology*; D016335:Zinc Fingers
D017456:Adenovirus Early Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D009856:Oncogene Proteins, Viral; D014157:Transcription Factors