Immunoelectron microscopic localization of the p21 protein in HT1080 human fibrosarcoma cell lines with altered N-ras gene expression.
The cellular localization of the p21 protein was studied by immunoelectron microscopy in human fibrosarcoma cells with different N-ras gene expression. The tumorigenic HT1080 cells-expressing equally the normal and mutant N-ras genes-contained the p21 in the plasma membrane, in pinocytotic areas as well as in the intracellular vesicles. The non-tumorigenic revertants-expressing more normal N-ras gene than the mutant one-contained p21 in the plasma membrane, especially at the cell junctions. These results suggest, that the mutant p21 may locate to the membrane areas connected with pino- or endocytotic functions.
Tímár
J
J
1st Institute of Pathology and Experimental Cancer Research, Semmelweis Medical University, Budapest, Hungary.
Paterson
H
H
D002455:Cell Division / Q000502:physiology; D005354:Fibrosarcoma / Q000737:chemistry*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D007124:Immunoenzyme Techniques; D016253:Microscopy, Immunoelectron; D016283:Proto-Oncogene Proteins p21(ras) / Q000032:analysis*; D014407:Tumor Cells, Cultured
C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)