Carbohydrate-binding protein 35 is the major cell-surface laminin-binding protein in colon carcinoma.
The interaction of tumor cells with laminin is thought to be critical in invasion and metastasis. We found that an endogenous lectin, carbohydrate-binding protein 35 (CBP-35), is the major laminin-binding protein on human colon carcinoma cells and that its surface expression suggests involvement in metastasis. We identified CBP-35 by laminin-affinity chromatography and immunoblotting. Surface expression of CBP-35 on eight human colon carcinoma cell lines was compared by flow cytometry. Poorly differentiated cell lines and DLD-2, a signet-ring carcinoma cell line, expressed more surface CBP-35 than well-differentiated cell lines. Poorly differentiated cell lines and DLD-2 are characterized as aggressive cell lines because they adhere to and invade through reconstituted basement membrane significantly better than well-differentiated cell lines. These data suggest that CBP-35 is involved in tumor cell-basement membrane interactions and that an increase in CBP-35 surface expression may facilitate metastatic potential of colon carcinoma cells.
Lee
E C
EC
Laboratory of Cancer Biology, Deaconess Hospital, Harvard Medical School, Boston, Mass 02115.
Woo
H J
HJ
Korzelius
C A
CA
Steele
G D
GD
Mercurio
A M
AM
D000943:Antigens, Differentiation / Q000032:analysis*; D002277:Carcinoma / Q000737:chemistry*; D002352:Carrier Proteins / Q000032:analysis*; D002846:Chromatography, Affinity; D003110:Colonic Neoplasms / Q000737:chemistry*; D005434:Flow Cytometry; D037502:Galectin 3; D006388:Hemagglutinins / Q000032:analysis*; D006801:Humans; D015151:Immunoblotting; D008565:Membrane Proteins / Q000032:analysis*; D009363:Neoplasm Proteins / Q000032:analysis*; D014407:Tumor Cells, Cultured
D000943:Antigens, Differentiation; D002352:Carrier Proteins; D037502:Galectin 3; D006388:Hemagglutinins; D008565:Membrane Proteins; D009363:Neoplasm Proteins