title: Structures and properties of vitellin isolated from Triatominae species.
abstract: A comparative study was conducted on the molecular weight, chemical composition, and immunological identity of vitellin (VN) from Triatominae species. VN is a lipoglycophosprotein: 12.76% carbohydrates, 12.53% lipids, and 0.6% phosphorus of the protein mass. Native VN shows one band of 310 kDa by gel filtration. VN shows a main band of 175 kDa by SDS-PAGE coincident with a vitellogenin (VG) band. Amino acid composition was very similar in the four species tested: T.infestans, T.pallidipennis, R.prolixus and D.maximus. T.infestans VN-VG has a total immunological identity with D.maximus female haemolymph and egg homogenates.
authors.affiliation: Instituto Nacional de Diagnóstico e Investigación de la Enfermedad de Chagas Dr M. Fatala Chaben, Buenos Aires, Argentina.
authors.forename: L E
authors.initials: LE
authors.lastname: Fichera
authors.forename: O D
authors.initials: OD
authors.lastname: Salomón
authors.forename: A M
authors.initials: AM
authors.lastname: Stoka
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008297:Male; D008970:Molecular Weight; D014225:Triatominae / Q000737:chemistry*; D014818:Vitellogenesis; D014819:Vitellogenins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; D014819:Vitellogenins