title: Prolongation of the kidney preservation period by simple cold storage up to 72 hours by human atrial natriuretic peptide.
abstract: To prolong the preservation period of donor kidneys, we tried to evaluate the effect of human atrial natriuretic peptide (hANP), which has vasodilator, natriuretic, and GFR-increasing effects. Autotransplantations were successful in 6 of 8 hANP-treated cases, but only one of 7 untreated cases (P less than 0.05) was successful. When removed kidneys were perfused prior to preservation, the gravity perfusion time was shorter in hANP-treated kidneys, resulting in rapid cooling. Renal biopsy 1 hr after transplantation revealed normal histology in the hANP-treated kidneys while a remarkable capillary collapse occurred in controls. A hANP-like immunoreactivity contents of biopsied tissue in the hANP-treated kidneys showed a higher value than in the controls, and cyclic GMP contents were also higher in the hANP-treated kidneys. Microangiography showed complete clarity, down to the peripheral vessels, in the hANP-treated kidneys, but not in the controls. Thus, the present study suggests that hANP is useful for prolonging the preservation time of donor kidneys.
authors.affiliation: Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Marumo
authors.forename: Y
authors.initials: Y
authors.lastname: Masaki
authors.forename: T
authors.initials: T
authors.lastname: Ida
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Ando
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis / Q000494:pharmacology*; D003080:Cold Temperature; D006152:Cyclic GMP / Q000032:analysis; D004285:Dogs; D005260:Female; D007668:Kidney / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D016030:Kidney Transplantation; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D012079:Renal Circulation; D013997:Time Factors; D014182:Transplantation, Autologous
chemical_list: D009320:Atrial Natriuretic Factor; D006152:Cyclic GMP