Inhibition of phosphatidylserine synthesis by phosphatidic acid in the Jurkat T cell line: role of calcium ions released from intracellular stores.
Phosphatidic acid exogenously added to Jurkat T-cells, provokes a marked inhibition of phosphatidylserine (PS) synthesis. Comparison of the efficiency of synthetic phosphatidic acids, differing in their fatty acid content, to induce inhibition of PS-synthesis have shown that short chain saturated and long chain unsaturated fatty acid-containing phosphatidic acids were the best inhibitors. Treatment of Jurkat cells with phosphatidic acid, induced a mobilization of calcium ions arising exclusively from intracellular stores, suggesting that Ca2+ from intracellular compartments might play a key role in the inhibition of PS synthesis. In activated cells, the use of R59022, a diacylglycerolkinase inhibitor, suggests that PA and probably calcium ions released by PA are the messengers responsible for the inhibition of PS synthesis in Jurkat T cells.
Pelassy
C
C
Immunologie Cellulaire et Moléculaire, INSERM U210, Nice, France.
Breittmayer
J P
JP
Mary
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D
Aussel
C
C
D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D017252:CD3 Complex; D002118:Calcium / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D015458:Leukemia, T-Cell / Q000378:metabolism / Q000473:pathology*; D008213:Lymphocyte Activation / Q000502:physiology; D010712:Phosphatidic Acids / Q000494:pharmacology*; D010718:Phosphatidylserines / Q000096:biosynthesis*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors; D011744:Pyrimidinones / Q000494:pharmacology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013329:Structure-Activity Relationship; D013844:Thiazoles / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D010712:Phosphatidic Acids; D010718:Phosphatidylserines; D010972:Platelet Activating Factor; D011744:Pyrimidinones; D011948:Receptors, Antigen, T-Cell; D013844:Thiazoles; C047505:R 59022; D002118:Calcium