title: Immunoassay method for the determination of immunoglobulin G using bacterial magnetic particles.
abstract: We have developed a novel immunoassay method using bacterial magnetic particles for the determination of immunoglobulin G (IgG). Fluorescein isothiocyanate (FITC) conjugated anti IgG-bacterial magnetic particles were prepared. The fluorescence quenching caused by agglutination of FITC-anti IgG antibody-bacterial magnetic particle conjugates was measured by using a fluorescence spectrophotometer. The aggregates based on specific immunoreaction were separated by a gelatin solution. The aggregation of bacterial magnetic particle conjugates was enhanced by application of a magnetic field. The relative fluorescence intensity correlated linearly with a concentration of IgG in the range 0.5-100 ng/mL.
authors.affiliation: Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Hashimoto
authors.forename: T
authors.initials: T
authors.lastname: Matsunaga
mesh_terms: D000371:Agglutination; D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D001419:Bacteria / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate; D015200:Fluoroimmunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis*; D008280:Magnetics; D051379:Mice
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D016650:Fluorescein-5-isothiocyanate