Editing of mitochondrial atp9 transcripts from two sorghum lines.
Genomic and cDNA sequences of the ATP synthase complex subunit 9 (atp9) genes from two sorghum lines were determined. Sequences of cDNAs revealed eight C to U transcript editing events resulting in six amino acid changes and a new stop codon which eliminated 12 carboxy-terminal residues, compared to the genomic sequence. Sorghum atp9 has a unique five-residue amino-extension relative to other higher plants. The resulting predicted 79-residue gene product has a molecular weight of 8.179 kDa. The predicted phe-val-phe carboxy-terminus is identical to that from cDNA sequences of wheat, Oenothera, and petunia. Partial editing of transcripts was detected in each sorghum line.
Salazar
R A
RA
Plant Pathology Department, University of Florida, Gainesville 32611.
Pring
D R
DR
Kempken
F
F
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA; D002523:Edible Grain / Q000235:genetics*; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D006180:Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012098:Reproduction; D012689:Sequence Homology, Nucleic Acid
D004247:DNA; D006180:Proton-Translocating ATPases