IL-2 and IL-5 both induce mu S and J chain mRNA in a clonal B cell line, but differ in their cell-cycle dependency for optimal signaling.
We have found that a neoplastic Lyl+ B cell clone (BCL1-3B3) can be stimulated to secrete IgM by a Th1-derived cytokine, IL-2, and/or by a Th2-derived cytokine, IL-5. At suboptimal concentrations these interleukins acted synergistically to enhance IgM secretion. Both IL-2 and IL-5 induced increases in microseconds and J chain mRNA levels. In the presence of both ILs, increases in microseconds and J chain mRNA were additive and paralleled increases in IgM secretion. Using cells synchronized at the G1/S border with excess thymidine or in early G1 using isoleucine-deficient media, IL-2 and IL-5 differed in their cell-cycle dependency for signal transmission. IL-5 appeared to act preferentially in late G1 of the cell cycle. In contrast, IL-2 stimulated S and G2 phase cells slightly more efficiently than cells in G1 of the cell cycle. Furthermore, a twofold increase in high-affinity IL-2R was observed as the cells entered S phase. The results suggest that although IL-2 and IL-5 can independently and additively induce differentiation of the Lyl+ BCL1-3B3 cells, they differ in their point of action during the cell cycle.
Takayasu
H
H
Genetics Program, Michigan State University, East Lansing 48824.
Brooks
K H
KH
D000818:Animals; D000921:Antibody-Producing Cells / Q000166:cytology / Q000502:physiology; D001402:B-Lymphocytes / Q000166:cytology / Q000502:physiology*; D015152:Blotting, Northern; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004357:Drug Synergism; D015870:Gene Expression / Q000187:drug effects; D005803:Genes, Immunoglobulin; D007144:Immunoglobulin J-Chains / Q000235:genetics*; D007075:Immunoglobulin M / Q000378:metabolism*; D007148:Immunoglobulin mu-Chains / Q000235:genetics*; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology*; D015848:Interleukin-5 / Q000494:pharmacology*; D008211:Lymphocyte Cooperation; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D015398:Signal Transduction; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D013997:Time Factors
D007144:Immunoglobulin J-Chains; D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains; D007376:Interleukin-2; D015848:Interleukin-5; D012333:RNA, Messenger