[Retinoic acid reduces the expression of nuclear receptors of triiodothyronin and inhibits an early stage of the differentiation in the Ob 17 preadipocytic strains].
In the murine preadipocyte cell line Ob 17, T3 is known as being necessary at an early step of adipose differentiation for the expression of late markers, but not necessary for the expression of early markers. These cells contain nuclear T3 receptors (RT3) which are products of c-erb A alpha proto oncogenes, and are down-regulated by T3. In these cells, retinoic acid (AR) inhibits the adipose differentiation and the expression of early and late markers, and T3 sensitizes the cells to the inhibitory action of AR. We also show that AR down-regulates the expression of RT3 at both pre- and post-translational levels. This inhibition is dependent on AR concentration, a peculiar sensitivity to AR being observed at an early step of development.
Teboul
M
M
INSERM U 38 et U 260*, Faculté de Médecine, Marseille.
Bismuth
J
J
Gharbi-Chihi
J
J
Valette
A
A
Bonne
J
J
Ghiringhelli
O
O
Torresani
J
J
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D015870:Gene Expression / Q000187:drug effects*; D051379:Mice; D014176:Protein Biosynthesis; D011988:Receptors, Thyroid Hormone / Q000187:drug effects* / Q000235:genetics; D014212:Tretinoin / Q000494:pharmacology*
D011988:Receptors, Thyroid Hormone; D014212:Tretinoin
L'acide rétinoïque diminue l'expression des récepteurs nucléaires de triiodothyronine et inhibe une étape précoce de la différenciation dans la lignée préadipocytaire Ob 17.