title: Effects of pH and cyclic adenosine monophosphate on ileal electrolyte transport in the rat and rabbit.
abstract: Alterations in extracellular pH cause reciprocal changes in NaCl absorption in the rat and rabbit ileum. The presence of cholera toxin-induced secretion does not affect pH action measured by in vivo perfusion of the rat ileum. We examined the interaction of pH and cyclic adenosine monophosphate-induced secretion in the rabbit ileum. We found that alterations in arterial pH did not affect ileal absorption in the rabbit in the presence of cholera toxin-induced secretion. This was true whether transport was studied during in vivo ileal perfusion of anesthetized rabbits or by measuring Na+ and Cl- fluxes across isolated, short-circuited tissues in the Ussing chamber. The effects of pH also were blocked when normal rabbit ileum was exposed to 1 mmol/L dibutyryl cyclic adenosine monophosphate in vitro. By contrast, alterations in bathing solution pH affected ileal absorption in the rat in the presence and absence of cyclic adenosine monophosphate. Similarly, exposure to cyclic adenosine monophosphate did not affect the response of the rat colon to PCO2. These findings suggest that the apparently independent effects of pH and cyclic adenosine monophosphate in the rat ileum are not universal. In tissues such as the rabbit ileum, the mechanisms of pH and cyclic adenosine monophosphate action may have biochemical or physiological pathways in common.
authors.affiliation: Department of Veterans Affairs Medical Center, New York, New York.
authors.forename: A N
authors.initials: AN
authors.lastname: Charney
authors.forename: D S
authors.initials: DS
authors.lastname: Goldfarb
authors.forename: R W
authors.initials: RW
authors.lastname: Egnor
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002712:Chlorides / Q000493:pharmacokinetics; D002772:Cholera Toxin / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology*; D004573:Electrolytes / Q000493:pharmacokinetics*; D006863:Hydrogen-Ion Concentration; D007082:Ileum / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D008297:Male; D010477:Perfusion; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000493:pharmacokinetics
chemical_list: D002712:Chlorides; D004573:Electrolytes; D003994:Bucladesine; D002772:Cholera Toxin; D012964:Sodium; D000242:Cyclic AMP