title: A method for steam-sterilizing semi-solid diets for germ-free mice research.
abstract: A semi-solid corn-meal diet sterilized in glass flasks in a hospital-type autoclave is described. The values of weight gain, feed efficiency, apparent digestibility, and cecal diameters were the same for germ-free mice fed either on this diet or on a soybean meal and alfalfa diet (L-485), widely used in germ-free research. The corn-meal diet has been successfully used for eight years.
authors.affiliation: Instituto de Ciencias Biológicas, Universidade Federal de Minas Gerais, Brazil.
authors.forename: T
authors.initials: T
authors.lastname: Moraes-Santos
authors.forename: J R
authors.initials: JR
authors.lastname: Nicoli
authors.forename: M E
authors.initials: ME
authors.lastname: Silva
authors.forename: E C
authors.initials: EC
authors.lastname: Vieira
mesh_terms: D000704:Analysis of Variance; D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002364:Caseins; D003314:Corn Oil; D005433:Flour; D005526:Food, Formulated; D005856:Germ-Free Life; D000455:Medicago sativa; D051379:Mice; D012107:Research Design; D013227:Steam; D013242:Sterilization / Q000379:methods*; D015430:Weight Gain; D003313:Zea mays
chemical_list: D002364:Caseins; D013227:Steam; D003314:Corn Oil