Ontogeny of dopamine D1 and D2 receptor subtypes in rat basal ganglia: a quantitative autoradiographic study.
The ontogeny of D1 and D2 dopamine (DA) receptors in rat basal ganglia was examined by quantitative autoradiography using the iodinated ligands [125I]SCH 23982 and [125I]iodobenzamide [( 125I]IBZM), respectively. Temporal and spatial differences in the development of the receptor subtypes were observed. Scatchard transformation of saturation isotherms conducted at postnatal day 10 (P10) and P60, showed that there was no age-related change in the affinity of [125I]SCH 23982 binding to D1 receptors (Kd = 2.6 nM) but there was a significant increase in the Bmax (771 compared to 2032 fmol/mg protein, P = 0.002). A statistically significant difference in Kd was noted between ages P10 and P60 for [125I]IBZM labelling of D2 receptors (0.62 vs 1.00 nM, respectively, P less than 0.01). A significant increase in the Bmax (211 and 721 fmol/mg protein, P less than 0.01) was also observed. D1 receptors were visible as distinct patches at P1. The highest density was found in the ventrolateral caudate-putamen (CPu). By P5 the patches were found in all subregions of the CPu and nucleus accumbens. Between P7 and P10 the binding became distinctly less patchy due to a marked increase in the density of D1 receptors in non-patch (matrix) regions. Adult levels of receptor were seen by P30. The concentration of DA (measured by HPLC) and binding of [3H]mazindol to DA uptake sites in whole striatum showed similar and nonlinear increases with age. The age-related change in the topography of binding sites for [3H]mazindol was similar to that of D1 receptors at the same ages. Both D2 receptors and [3H]hemicholinium-3 (HC-3) binding to high affinity transport sites for choline developed initially in the dorso-lateral CPu. Their topography was largely overlapping but distinct from that of the D1 receptor. D2 receptors were not consistently observed until P3 in the CPu, and zones of enriched binding were aligned with zones of low density for D1 receptors. The density of D2 receptors reached adult levels by P30. The differential development of the DA receptors was also evident in the substantia nigra (SN) and globus pallidus (GP). D1 receptors were found in SN prior to the appearance of D2 receptors and throughout development the density was greater in pars reticulata than in pars compacta, whereas the density of D2 receptors was higher in the pars compacta. At all ages the density of D1 receptors was greater than the density of D2 receptors in the GP and reached adult levels before reaching it in the CPu or SN.(ABSTRACT TRUNCATED AT 400 WORDS)
Rao
P A
PA
Department of Psychiatry, University of Pennsylvania School of Medicine, Philadelphia 19104-6141.
Molinoff
P B
PB
Joyce
J N
JN
D000375:Aging; D000818:Animals; D001345:Autoradiography; D001479:Basal Ganglia / Q000254:growth & development* / Q000378:metabolism; D001549:Benzamides / Q000378:metabolism*; D001552:Benzazepines / Q000031:analogs & derivatives* / Q000378:metabolism; D018492:Dopamine Antagonists; D005260:Female; D006426:Hemicholinium 3 / Q000378:metabolism; D007457:Iodine Radioisotopes; D007700:Kinetics; D008297:Male; D008454:Mazindol / Q000378:metabolism; D009928:Organ Specificity; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine / Q000378:metabolism*; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D014316:Tritium
C049558:8-iodo-2,3,4,5-tetrahydro-3-methyl-5-phenyl-1H-3-benzazepine-7-ol; D001549:Benzamides; D001552:Benzazepines; D018492:Dopamine Antagonists; D007457:Iodine Radioisotopes; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D014316:Tritium; D006426:Hemicholinium 3; C037689:benzamide; D008454:Mazindol