Prolongation of the kidney preservation period by simple cold storage up to 72 hours by human atrial natriuretic peptide.
To prolong the preservation period of donor kidneys, we tried to evaluate the effect of human atrial natriuretic peptide (hANP), which has vasodilator, natriuretic, and GFR-increasing effects. Autotransplantations were successful in 6 of 8 hANP-treated cases, but only one of 7 untreated cases (P less than 0.05) was successful. When removed kidneys were perfused prior to preservation, the gravity perfusion time was shorter in hANP-treated kidneys, resulting in rapid cooling. Renal biopsy 1 hr after transplantation revealed normal histology in the hANP-treated kidneys while a remarkable capillary collapse occurred in controls. A hANP-like immunoreactivity contents of biopsied tissue in the hANP-treated kidneys showed a higher value than in the controls, and cyclic GMP contents were also higher in the hANP-treated kidneys. Microangiography showed complete clarity, down to the peripheral vessels, in the hANP-treated kidneys, but not in the controls. Thus, the present study suggests that hANP is useful for prolonging the preservation time of donor kidneys.
Marumo
F
F
Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Masaki
Y
Y
Ida
T
T
Sato
K
K
Ando
K
K
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis / Q000494:pharmacology*; D003080:Cold Temperature; D006152:Cyclic GMP / Q000032:analysis; D004285:Dogs; D005260:Female; D007668:Kidney / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D016030:Kidney Transplantation; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D012079:Renal Circulation; D013997:Time Factors; D014182:Transplantation, Autologous
D009320:Atrial Natriuretic Factor; D006152:Cyclic GMP