CD5+ B cells and CD4-8-T cells in neuroimmunological diseases.
Using 2- and 3-colour FACS analysis we found increased levels of fetal-type CD5+ B cells and CD4-8- T cells in cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS) and aseptic meningitis (AM) compared to control probands with muscular tension headache (TH). Similar differences were found for CD5+ B cells in peripheral blood, but at lower levels. CD4-8- T cells in blood exceeded those in CSF in all patient groups, with the exception of relapsing remitting MS, revealing the highest values in AM. There was a positive correlation between CD4-8- T cells and T cell receptor (TCR) gamma delta bearing T cells in blood and CSF. The double-negative T cells exceeded the TCR gamma delta T cells by about 1%. A positive correlation between CD5+ B cells and CD4-8- T cell level in CSF was found in MS and AM, but not in TH, nor in blood of any patient group. HLA-DR expression was lower in CD5+ B cells than in CD5- B cells. We conclude that fetal-type lymphocytes are enriched in CSF compartment of patients with inflammatory diseases of the central nervous system, irrespective of autoimmune mechanisms involved, but the function of CD5+ B cells is mainly to produce the autoantibodies.
Correale
J
J
Department of Neurology, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden.
Mix
E
E
Olsson
T
T
Kostulas
V
V
Fredrikson
S
S
Höjeberg
B
B
Link
H
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000134:cerebrospinal fluid*; D001402:B-Lymphocytes; D005260:Female; D006261:Headache / Q000134:cerebrospinal fluid* / Q000276:immunology / Q000473:pathology; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008582:Meningitis, Aseptic / Q000134:cerebrospinal fluid* / Q000276:immunology / Q000473:pathology; D008875:Middle Aged; D009468:Neuromuscular Diseases / Q000134:cerebrospinal fluid* / Q000276:immunology / Q000473:pathology; D013601:T-Lymphocytes; D006377:T-Lymphocytes, Helper-Inducer; D050378:T-Lymphocytes, Regulatory
D015703:Antigens, CD