Modulation of murine host-versus-graft disease by anti-CD3 monoclonal antibody.
BALB/c mice made tolerant to A/J alloantigens by neonatal injection of (A/J x BALB/c)F1 spleen cells develop a host-versus-graft (HVG) disease due to the activation of donor B cells by a subset of host alloreactive helper T cells. We have investigated the effects of a single neonatal injection of the 145-2C11 anti-mouse CD3 monoclonal antibody (MoAb) on the establishment of allotolerance and on the development of the immunopathological features of HVG disease. First, this treatment did not modify the specific anti-donor cytotoxic T lymphocyte (CTL) unresponsiveness or the persistence of circulating immunoglobulins bearing donor allotype. Second, the hyper IgE, the hyper IgG1 and the increased expression of Ia antigens on B cells found in untreated HVG mice were not observed after injection of the 145-2C11 MoAb. Likewise, treated mice displayed lower levels of anti-DNA IgG antibodies and less glomerular immune deposits as compared with untreated HVG mice. We conclude that the administration of the anti-CD3 MoAb did not interfere with the induction of allotolerance but exerts a pronounced inhibitory effect on the associated immunopathological syndrome.
Wissing
M
M
Laboratoire Pluridisciplinaire de Recherche Expérimentale Biomédicale, Université Libre de Bruxelles, Brussels, Belgium.
Marchant
A
A
Moser
M
M
Flamand
V
V
Leo
O
O
Abramowicz
D
D
Urbain
J
J
Goldman
M
M
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D000974:Antibodies, Antinuclear / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D017252:CD3 Complex; D005921:Glomerulonephritis / Q000517:prevention & control; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D006789:Host vs Graft Reaction / Q000276:immunology*; D007108:Immune Tolerance; D007136:Immunoglobulins / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013154:Spleen / Q000637:transplantation*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D000949:Histocompatibility Antigens Class II; D007136:Immunoglobulins; D011948:Receptors, Antigen, T-Cell