Indomethacin modulation of monocyte cytokine release following pelvic irradiation for cancer.
Pelvic irradiation for urogenital cancer reduced monocyte release of tumour necrosis factor alpha (TNF-alpha). Addition of indomethacin to monocyte cultures increased TNF-alpha production after but not before irradiation. E. coli lipopolysaccharide (LPS) increased TNF-alpha release before as well as after radiation therapy and addition of indomethacin to LPS-stimulated monocytes further increased TNF-alpha production following radiotherapy. Spontaneous interleukin-1 (IL-1) release was increased in the cancer patients and was not significantly affected by radiation therapy. LPS increased IL-1 release before as well as after irradiation, but indomethacin did not further change IL-1 secretion. These findings suggest that prostaglandins differentially regulate TNF-alpha and IL-1 release. Administration of cyclo-oxygenase inhibitors during radiation therapy might increase TNF-alpha release in vivo and thereby enhance the host defence against tumours.
Petrini
B
B
Central Microbiological Laboratory, Stockholm County Council, Sweden.
Wolk
G
G
Wasserman
J
J
Vedin
I
I
Strannegård
O
O
Blomgren
H
H
Esposti
P L
PL
D000368:Aged; D004926:Escherichia coli; D005260:Female; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology*; D007375:Interleukin-1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D010388:Pelvis; D011471:Prostatic Neoplasms / Q000532:radiotherapy*; D015203:Reproducibility of Results; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism* / Q000528:radiation effects; D001749:Urinary Bladder Neoplasms / Q000532:radiotherapy
D007375:Interleukin-1; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D007213:Indomethacin