[Arterioarterial shunt coated with collagen. A model of acute arterial thrombosis for testing antithrombotic agents in rats].
We developed a new thrombosis model in Wistar rats. It consists of a shunt of which the central part is coated with collagen, a thrombogenic protein, implanted between the two carotid arteries of the rat. The occlusion time of the shunt is measured by a thermic method. The composition of the thrombus is analyzed by double isotope labeling of platelets and fibrinogen. Four antithrombotic drugs were studied: two antiplatelet agents, aspirin and clopidogrel; two anticoagulants, heparin and recombinant hirudin. The results show that aspirin alone does not increase the occlusion time of the shunt. Clopidogrel, heparin and hirudin at doses of 50 mg/kg, 500 IU/kg and 5 mg/kg significantly increase the thrombosis time but the composition of the thrombus varies according to the nature of the drug: antiplatelet (clopidogrel) or anticoagulant (heparin, hirudin). This model enables a plurifactorial analysis of acute arterial thrombosis and should be of value to discriminate the effects of antithrombotic and thrombolytic agents.
Mantz
F
F
Service d'Hémostase et de Thrombose, INSERM U 311, Centre régional de Transfusion sanguine, Strasbourg.
Freund
M
M
Cazenave
J P
JP
D000818:Animals; D001241:Aspirin / Q000627:therapeutic use; D002339:Carotid Arteries / Q000601:surgery*; D000077144:Clopidogrel; D003094:Collagen / Q000008:administration & dosage; D004195:Disease Models, Animal; D005402:Fistula / Q000209:etiology* / Q000503:physiopathology; D006083:Graft Occlusion, Vascular / Q000503:physiopathology; D008297:Male; D008954:Models, Biological; D010975:Platelet Aggregation Inhibitors / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015912:Thrombolytic Therapy; D013927:Thrombosis / Q000188:drug therapy / Q000503:physiopathology*; D013988:Ticlopidine / Q000031:analogs & derivatives / Q000627:therapeutic use
D010975:Platelet Aggregation Inhibitors; D003094:Collagen; D000077144:Clopidogrel; D013988:Ticlopidine; D001241:Aspirin
Shunt artérioartériel recouvert de collagène. Un modèle de thrombose artérielle aiguë pour tester les antithrombotiques chez le rat.