title: Age-related differences in synaptosomal peroxidative damage and membrane properties.
abstract: Young, adult, and old rats were used to study the effect of age on the integrity and functioning of brain synaptosomes. An evaluation was made of the differences in lipid composition, membrane fluidity, Na+, K(+)-ATPase activity, and susceptibility to in vitro lipid peroxidation. There was an age-related increase in synaptosomal free fatty acids, with no modification in acyl chain composition, and a decrease in membrane phospholipids which increased the cholesterol/phospholipid mole ratio. With altered lipid composition, there was a corresponding age-dependent decrease in membrane fluidity, a reduction of Na+, K(+)-ATPase activity, and an overall greater susceptibility to in vitro lipid peroxidation. Furthermore, lipid peroxidation promoted strong modifications of the membrane fluidity, lipid composition, and Na+,K(+)-ATPase activity just as aging did, thus indicating a possible contribution of oxidative damage to ageing processes. The cases studied revealed that the greater responsiveness of old membranes to in vitro lipid peroxidation resulted in the highest degree of membrane alteration, indicating that all pathological states known to promote a peroxidative injury can have even more dramatic consequences when they take place in old brain.
authors.affiliation: Department of Medical Chemistry and Biochemistry, Faculty of Medicine, University of Milan, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Viani
authors.forename: G
authors.initials: G
authors.lastname: Cervato
authors.forename: A
authors.initials: A
authors.lastname: Fiorilli
authors.forename: B
authors.initials: B
authors.lastname: Cestaro
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001921:Brain / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology; D002784:Cholesterol / Q000378:metabolism; D004161:Diphenylhexatriene; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D007501:Iron / Q000494:pharmacology; D007700:Kinetics; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000378:metabolism; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013574:Synaptosomes / Q000502:physiology*; D013858:Thiobarbiturates
chemical_list: D005230:Fatty Acids, Nonesterified; D008563:Membrane Lipids; D010743:Phospholipids; D013858:Thiobarbiturates; D004161:Diphenylhexatriene; D008315:Malondialdehyde; D002784:Cholesterol; D007501:Iron; D000254:Sodium-Potassium-Exchanging ATPase; C029684:thiobarbituric acid; D001205:Ascorbic Acid