[Production and characterization of monoclonal antibody against colon cancer associated antigen in Chinese].
Although many monoclonal antibodies have been made in human colon cancer, none of them are from the Chinese species. Recently, a colon cancer cell line CC-M2 established from a Chinese patient has been completely characterized and used as immunogen to produce monoclonal antibodies. Monoclonal antibodies were produced by standard hybridoma technique. The fusion rate was 95.8%. An isotype IgG1 of high proliferation named as Sam-2 was used in this study. The titers were measured around 10(4). Further studies on MoAb Sam-2 through indirect immunofluorescent and immunoperoxidase tests revealed its good specificity and sensitivity in colorectal cancer tissue. In CEA study, the result indicated that Sam-2 may react on a non-CEA related antigen. For further clinical application, the antigen was identified as a glycoprotein by chemical resistant test. In preliminary studies using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting techniques, Sam-2 could recognize two closed antigens or a dimer antigen with molecular weight 25.2 and 27 Kd respectively.
Cheng
J Y
JY
Department of Surgery and Medical Research, Tri-Service General Hospital, Taipei, R.O.C.
Lin
J C
JC
Meng
C L
CL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000951:Antigens, Neoplasm / Q000276:immunology* / Q000302:isolation & purification; D044466:Asian People; D003110:Colonic Neoplasms / Q000276:immunology*; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D009077:Mucins; D013624:Taiwan
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D009077:Mucins; C042384:gastro-intestinal mucus-associated antigens