A nuclease from rat-liver nuclei with endo- and exonucleolytic activity.
An Mg2(+)-dependent endonuclease endogenous to rat-liver nuclei had an exonuclease activity for single-stranded DNA, but not for duplex DNA. The activity was about twice as high in the 3'----5' direction as in the 5'----3' direction. The products by 3'----5' activity were mononucleotides alone. The 5'----3' activity released mononucleotides as main products and small amounts of di-, tri-, tetra- and oligonucleotides. Another major endonuclease endogenous to the nuclei, a Ca2+/Mg2(+)-dependent endonuclease, did not have such exonuclease activities.
Hibino
Y
Y
Cell Biology Division, Faculty of Pharmaceutical Sciences, Toyama Medical & Pharmaceutical University, Japan.
Iwakami
N
N
Sugano
N
N
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology*; D004247:DNA; D004277:DNA, Single-Stranded; D003854:Deoxyribonucleotides / Q000302:isolation & purification; D004720:Endonucleases / Q000378:metabolism*; D005092:Exonucleases / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D051381:Rats; D013379:Substrate Specificity
D004277:DNA, Single-Stranded; D003854:Deoxyribonucleotides; D004247:DNA; D004720:Endonucleases; D005092:Exonucleases