Proviral sequences detected by polymerase chain reaction in peripheral blood cells of horses with equine infectious anemia lentivirus.
Proviral sequences in the peripheral blood mononuclear cells of 3 horses with acute equine infectious anemia virus were monitored using the polymerase chain reaction. Provirus was detected during the initial viremic episode in each horse and during each of 3 relapsing viremic cycles, although the appearance of provirus lagged behind the onset of viremia. Following each viremic episode, provirus levels in the peripheral monocytes decreased to less than 1 copy in 5 x 10(6) cells.
O'Rourke
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KI
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman.
Besola
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ML
McGuire
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TC
D000818:Animals; D001483:Base Sequence; D015342:DNA Probes; D004279:DNA, Viral / Q000097:blood*; D004859:Equine Infectious Anemia / Q000097:blood / Q000382:microbiology*; D006736:Horses; D004860:Infectious Anemia Virus, Equine / Q000235:genetics / Q000302:isolation & purification*; D007963:Leukocytes, Mononuclear / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D015342:DNA Probes; D004279:DNA, Viral