title: Genome organization of membrane-containing bacteriophage PRD1.
abstract: We have determined the nucleotide sequence of the late region (11 kbp) of the lipid-containing bacteriophage PRD1. Gene localization was carried out by complementing nonsense phage mutants with genomic clones containing specific reading frames. The localization was confirmed by sequencing the N-termini of isolated gene products as well as sequencing the N-termini of tryptic fragments of the phage membrane-associated proteins. This, with the previously obtained sequence of the early regions, allowed us to organize most of the phage genes in the phage genome.
authors.affiliation: Department of Genetics, University of Helsinki, Finland.
authors.forename: J K
authors.initials: JK
authors.lastname: Bamford
authors.forename: A L
authors.initials: AL
authors.lastname: Hänninen
authors.forename: T M
authors.initials: TM
authors.lastname: Pakula
authors.forename: P M
authors.initials: PM
authors.lastname: Ojala
authors.forename: N
authors.initials: N
authors.lastname: Kalkkinen
authors.forename: M
authors.initials: M
authors.lastname: Frilander
authors.forename: D H
authors.initials: DH
authors.lastname: Bamford
mesh_terms: D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D005816:Genetic Complementation Test; D006090:Gram-Negative Bacteria; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D015183:Restriction Mapping; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins; C118469:assembly factor P17