title: Geographical and age-related variation in the activity of some enzymes associated with insecticide detoxification in sandflies (Diptera: Phlebotominae).
abstract: The activity of glutathione s-transferase (GST), glucose 6-phosphate dehydrogenase (G6PD), and esterases (EST1, EST2) was measured using microtitre plate assays of laboratory colonised Phlebotomus papatasi originating in Afghanistan, Cyprus, India, Iraq, Saudi Arabia, Spain and Tunisia. The mean enzyme activity of the seven populations was significantly different. Enzyme activity did not vary significantly with age in sandflies, unlike some mosquitoes. A baseline enzyme activity for susceptibility is proposed for P. papatasi.
authors.affiliation: Public Health Division, National Institute of Health, Islamabad, Pakistan.
authors.forename: M A
authors.initials: MA
authors.lastname: Munir
authors.forename: J
authors.initials: J
authors.lastname: Hemingway
authors.forename: R P
authors.initials: RP
authors.lastname: Lane
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D000852:Anopheles / Q000201:enzymology / Q000502:physiology; D004950:Esterases / Q000378:metabolism*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D008658:Inactivation, Metabolic; D007306:Insecticides / Q000493:pharmacokinetics*; D010691:Phlebotomus / Q000201:enzymology / Q000502:physiology; D011576:Psychodidae / Q000201:enzymology* / Q000502:physiology; D013045:Species Specificity
chemical_list: D007306:Insecticides; D005954:Glucosephosphate Dehydrogenase; D005982:Glutathione Transferase; D004950:Esterases