Evaluation of adenovirus type 4 and type 7 recombinant hepatitis B vaccines in dogs.
Recombinant hepatitis B virus vaccines based on adenovirus (Ad) vectors Ad4 and Ad7 have been prepared. However, immunogenicity testing of such vaccines in experimental animals is difficult because these human adenoviruses exhibit a highly restricted host range. In this study, the dog was evaluated as a model for screening Ad4- and Ad7-vectored vaccines. Intratracheal inoculation of dogs with Ad4 and Ad7 induced substantial type-specific humoral immune responses that were significantly higher than responses obtained following pharyngeal or oral inoculations. Inoculation of dogs with recombinant Ad7 and Ad4 vaccines expressing hepatitis B surface antigen (HBsAg) elicited large antibody responses to HBsAg (anti-HBs). Substantial secondary anti-HBs responses were produced upon sequential immunizations with heterotypic Ad7 and Ad4 recombinant vaccines. These data thus indicate that the dog is a useful model for evaluating immune responses to vaccines based on Ad4 and Ad7 vectors.
Chengalvala
M
M
Biotechnology and Microbiology Division, Wyeth-Ayerst Research, Philadelphia, PA 19101.
Lubeck
M D
MD
Davis
A R
AR
Mizutani
S
S
Molnar-Kimber
K
K
Morin
J
J
Hung
P P
PP
D000260:Adenoviruses, Human / Q000276:immunology* / Q000502:physiology; D000818:Animals; D000915:Antibody Affinity; D004285:Dogs; D006510:Hepatitis B Antibodies / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D017325:Hepatitis B Vaccines; D006801:Humans; D007700:Kinetics; D014407:Tumor Cells, Cultured; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology*; D014761:Viral Hepatitis Vaccines / Q000276:immunology*; D010948:Viral Plaque Assay; D014779:Virus Replication
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D014614:Vaccines, Synthetic; D014761:Viral Hepatitis Vaccines