title: Synthesis of site-specifically modified oligoribonucleotides for studies of the recognition of TAR RNA by HIV-1 tat protein and studies of hammerhead ribozymes.
abstract: Synthetic oligoribonucleotides having single uracil residues replaced by dU, dT, 2'-O-methylU or 5-bromodU have been prepared and used in the study of the interaction of HIV-1 tat protein with an RNA stem-loop. The preparation of phosphoramidites of 5-bromouridine and purine riboside suitable for use in solid-phase oligoribonucleotide synthesis is also described. The effect of adenine replacement by purine in a hammerhead ribozyme has also been determined.
authors.affiliation: MRC Laboratory of Molecular Biology, Cambridge, UK.
authors.forename: G
authors.initials: G
authors.lastname: Slim
authors.forename: C
authors.initials: C
authors.lastname: Pritchard
authors.forename: E
authors.initials: E
authors.lastname: Biala
authors.forename: U
authors.initials: U
authors.lastname: Asseline
authors.forename: M J
authors.initials: MJ
authors.lastname: Gait
mesh_terms: D001483:Base Sequence; D015696:Gene Products, tat / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism*; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D016337:RNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D014498:Uracil / Q000031:analogs & derivatives*; D054322:tat Gene Products, Human Immunodeficiency Virus; D000099253:Trans-Activation Responsive RNA-Binding Protein
chemical_list: D015696:Gene Products, tat; D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D016337:RNA, Catalytic; D016601:RNA-Binding Proteins; D014498:Uracil; D054322:tat Gene Products, Human Immunodeficiency Virus; D000099253:Trans-Activation Responsive RNA-Binding Protein