Comparison of biological and immunochemical properties indicates that scatter factor and hepatocyte growth factor are indistinguishable.
Scatter factor, a stimulant of epithelial cell motility, and Hepatocyte Growth Factor (HGF) were compared by cross-biological studies using naturally occurring and recombinant proteins in four bioassays. Both scatter factor and HGF produced similar effects in cell motility and DNA synthesis assays. Antibodies to scatter factor or HGF neutralized the biological activities of each cytokine, and in immunoblotting reacted with species of the same Mr. These results, together with the available sequence data, suggest that scatter factor and HGF are the same protein.
Furlong
R A
RA
University of Cambridge, Department of Pathology, UK.
Takehara
T
T
Taylor
W G
WG
Nakamura
T
T
Rubin
J S
JS
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000737:chemistry* / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D006133:Growth Substances / Q000737:chemistry* / Q000494:pharmacology; D017228:Hepatocyte Growth Factor; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D011994:Recombinant Proteins
D016207:Cytokines; D006133:Growth Substances; D011994:Recombinant Proteins; D017228:Hepatocyte Growth Factor; D004247:DNA