Physical mapping of the human genome by pulsed field gel analysis.
Detailed physical maps of large regions of the human genome are important for locating and cloning genes responsible for human hereditary diseases, as well as for obtaining a more detailed understanding of chromosome structure and evolution. Pulsed field gel electrophoresis provides one method for generating physical maps of non-methylated rare restriction endonuclease sites. This review summarizes recent progress in the isolation of region-specific mapping probes and in their application for the physical mapping of selected regions of the human genome.
Evans
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GA
Molecular Genetics Laboratory, Salk Institute for Biological Studies, San Diego, California 92138.
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D004247:DNA / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D030342:Genetic Diseases, Inborn / Q000235:genetics; D008040:Genetic Linkage; D016045:Human Genome Project; D006801:Humans; D006822:Hybrid Cells; D008285:Major Histocompatibility Complex; D009693:Nucleic Acid Hybridization; D012377:Rodentia
D004247:DNA