Mutant strains of Streptomyces cinnamonensis protoplasts. Cultural and physiological conditions.
Mycelium of Streptomyces cinnamonensis mutant strains cultivated in a synthetic medium with glycine produced protoplasts after lysis of cell walls with lysozyme. The protoplast yield was up to 95%. The protoplasts could revert and mycelial forms were thus regenerated. In a sucrose-containing medium the protoplasts stored at 4 degrees C were stable for 2 d.
Matĕjů
J
J
Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
Marsálková
J
J
Nohýnek
M
M
Steinerová
N
N
D003470:Culture Media; D005998:Glycine; D009113:Muramidase; D009154:Mutation; D009172:Mycology / Q000379:methods; D011523:Protoplasts / Q000166:cytology / Q000502:physiology; D013302:Streptomyces / Q000166:cytology / Q000235:genetics* / Q000502:physiology
D003470:Culture Media; D009113:Muramidase; D005998:Glycine