Effect of hairpin DNA on poly(phenylalanine) synthesis.
In a poly(U) dependent poly(Phe) synthesis, an unaminoacylated tRNA(phe) binds to a ribosome and inhibits poly(Phe) synthesis in excess tRNA(Phe). A small DNA fragment having a hairpin structure was added to this system and was found to prevent an unaminoacylated tRNA(phe) from binding to a ribosome and enhance the efficiency of poly(Phe) synthesis in the presence of excess tRNA(phe).
Yoshizawa
S
S
Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan.
Hirao
I
I
Miura
K
K
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010452:Peptide Biosynthesis; D010455:Peptides; D012360:RNA, Transfer, Phe / Q000378:metabolism
D010455:Peptides; D012360:RNA, Transfer, Phe; C012001:polyphenylalanine; D004247:DNA