title: Spot-scan imaging in transmission electron microscopy.
abstract: The determination of the structure of proteins and other organic materials by transmission electron microscopy is a rapidly developing field. Obtaining high-resolution images of these radiation-sensitive specimens has, until recently, been problematic. The development of spot-scan imaging, in which the electron beam is focused to a spot with a diameter of about 1000 angstroms and moved over the specimen to record the image, has overcome some of the most severe problems, which result from beam-induced motion of the specimen and its image. Elimination of this motion greatly enhances the contrast of high-resolution features of the image and promises a significant increase in the speed with which future structural work can be accomplished.
authors.affiliation: Donner Laboratory, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.
authors.forename: K H
authors.initials: KH
authors.lastname: Downing
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry; D003460:Crystallization; D005583:Fourier Analysis; D008854:Microscopy, Electron / Q000379:methods*; D015394:Molecular Structure; D010232:Paraffin / Q000737:chemistry; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry; D011095:Polyethylenes / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D018272:Porins; D011506:Proteins / Q000737:chemistry; D013876:Thiophenes / Q000737:chemistry; D014027:Tobacco Mosaic Virus / Q000648:ultrastructure
chemical_list: D001425:Bacterial Outer Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D011095:Polyethylenes; D011108:Polymers; D018272:Porins; D011506:Proteins; D013876:Thiophenes; C066730:polythiophene; D010232:Paraffin