The quality control of cell banks using DNA fingerprinting.
Reproducibility in animal cell culture technology requires careful preparation and characterisation of banks of cell cultures. The two standard techniques used in the quality control of such banks are isoenzyme analysis and cytogenetics which require complex and time-consuming procedures to enable cell line identification. However, DNA fingerprinting is potentially a more powerful method of analysis which can detect mutation and intra-species cross-contamination. At the European Collection of Animal Cell Cultures (ECACC) multilocus fingerprint analysis using probes 33.6 and 33.15 has been assessed in the quality control of cell banks. This method has confirmed consistency between master and working banks, has proven useful over a wide species range and can differentiate closely related cell lines. The key advantage of this method is its ability to detect cross-contamination by cell lines from a wide range of species using a straightforward and economical test. In addition the reproducibility of DNA fingerprints indicates their possible role in cell line authentication procedures which are important for patent and product licence applications.
Stacey
G N
GN
European Collection of Animal Cell Cultures, PHLS Centre for Applied Microbiology and Research, Salisbury, Wiltshire, Great Britain.
Bolton
B J
BJ
Doyle
A
A
D000818:Animals; D002460:Cell Line; D016172:DNA Fingerprinting; D006801:Humans; D011786:Quality Control