title: The evaluation of various methods and antigens for the detection of antibodies against Pityrosporum ovale in patients with seborrhoeic dermatitis.
abstract: Sera from 10 patients with seborrhoeic dermatitis and from 10 age-matched healthy individuals were examined for IgG activity against Pityrosporum ovale. The IgG activity was analysed using the following techniques: an enzyme-linked immunosorbent assay (ELISA) against whole P. ovale cells, purified cell-wall carbohydrate or protein extract, an indirect slide-immunofluorescence assay and fluorescence-activated flow cytometry using the whole organism as antigen. The ELISA method using the protein antigen was the only technique that showed a significant difference between patients and controls; a lower antibody response was found in the seborrhoeic dermatitis patients compared to healthy controls.
authors.affiliation: Department of Dermatology, University of Gothenburg, Sahlgrenska Hospital, Sweden.
authors.forename: I M
authors.initials: IM
authors.lastname: Bergbrant
authors.forename: S
authors.initials: S
authors.lastname: Johansson
authors.forename: D
authors.initials: D
authors.lastname: Robbins
authors.forename: K
authors.initials: K
authors.lastname: Bengtsson
authors.forename: J
authors.initials: J
authors.lastname: Faergemann
authors.forename: A
authors.initials: A
authors.lastname: Scheynius
authors.forename: T
authors.initials: T
authors.lastname: Söderström
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000908:Antibodies, Fungal / Q000032:analysis*; D012628:Dermatitis, Seborrheic / Q000276:immunology / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008290:Malassezia / Q000276:immunology*; D008297:Male; D008875:Middle Aged
chemical_list: D000908:Antibodies, Fungal