title: [Determination of trimethoprim and sulfamethoxazole in pharmaceutical preparations by non-relative component reference-multiplier derivative spectrophotometry].
abstract: This paper provides the basic principle and experimental technique of non-relative component reference-multiplier derivative spectrophotometry. The procedure overcomes the problem of overlapping in derivative spectrophotometry and quantitative analysis of lower content component in mixture can be done without separation. This method was investigated to assay the contents of trimethoprim (TMP) and used to assay the zero crossing derivative spectrophotometry for the assay of sulfamethoxazole (SMZ) in pharmaceutical preparations. The average recoveries of TMP and SMZ were 102.5 +/- 1.63% (CV) and 100.3 +/- 0.99% (CV) respectively. The results show that it not only can effectively remove the interference from each other, but also give a higher sensitivity and accuracy.
authors.affiliation: Weifang Institute for Drug Control.
authors.forename: L M
authors.initials: LM
authors.lastname: Lin
mesh_terms: D004338:Drug Combinations; D007267:Injections; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013420:Sulfamethoxazole / Q000032:analysis*; D013607:Tablets; D014295:Trimethoprim / Q000032:analysis*
chemical_list: D004338:Drug Combinations; D013607:Tablets; D014295:Trimethoprim; D013420:Sulfamethoxazole