title: Long-term potentiation induced changes in protein synthesis of hippocampal subfields of freely moving rats: time-course.
abstract: The incorporation of L-[U-14C]leucine into proteins of cytosolic (soluble proteins) and of the 100,000 x g pellet fraction from ipsilateral hippocampal subfields (CA1, CA3, CA4/area dentata) of rats was studied during and at various times after electrical tetanisation of the right perforant pathway using topical precursor applications and 1 h incorporation periods. Rates of protein synthesis were estimated calculating the relative specific radioactivity of proteins (RSA). The RSA-values of both protein fractions estimated in the ipsilateral CA4/area dentata were found to be about two times higher in tetanised rats compared to either passive or stimulated controls (p less than 0.05) when leucine was administered 5 min after tetanisation. Under these conditions, no differences between tetanised and control animals were observed in all other hippocampal subfields studied. No differences in RSA-values evaluated in CA4/area dentata between tetanised and passive controls were detected when leucine was administered immediately prior to tetanisation. From 2 h to 8 h after induction of LTP, protein synthesis in CA4/area dentata of tetanised rats appeared to be decreased (p less than 0.05) compared to passive controls. These results provide evidence for a transient LTP-induced augmentation of protein synthesis in the targets which might be an essential prerequisite for those structural changes realising the long-lasting enhanced efficacy of synaptic transmission at perforant path/granular cells connections.
authors.affiliation: Institute of Pharmacology and Toxicology, Medical Academy Magdeburg, FRG.
authors.forename: S
authors.initials: S
authors.lastname: Frey
authors.forename: C
authors.initials: C
authors.lastname: Schwiegert
authors.forename: M
authors.initials: M
authors.lastname: Krug
authors.forename: B
authors.initials: B
authors.lastname: Lössner
mesh_terms: D000818:Animals; D002250:Carbon Radioisotopes; D003600:Cytosol / Q000378:metabolism; D004558:Electric Stimulation; D005071:Evoked Potentials; D006624:Hippocampus / Q000378:metabolism / Q000502:physiology*; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D011712:Pyramidal Tracts / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013997:Time Factors
chemical_list: D002250:Carbon Radioisotopes; D009419:Nerve Tissue Proteins; D007930:Leucine