cis-acting DNA elements responsive to gibberellin and its antagonist abscisic acid.
We have used a transient expression assay in aleurone protoplasts of barley to delineate hormone response elements of the abscisic acid (ABA)-responsive rice gene Rab16A and of the gibberellin A3 (GA3)-responsive barley alpha-amylase gene Amy 1/6-4. Our approach used transcriptional fusions between their 5' upstream sequences and a bacterial chloramphenicol acetyltransferase reporter gene. A chimeric promoter containing six copies of the -181 to -171 region of Rab 16A fused to a minimal promoter conferred ABA-responsive expression on the reporter gene. Transcription from this ABA response element (GTACGTGGCGC) was unaffected by GA3. A chimeric promoter containing six copies of the -148 to -128 sequence of Amy 1/6-4 fused to the minimal promoter conferred GA3-responsive expression on the reporter gene. Transcription from this GA3 response element (GGCCGATAACAAACTCCGGCC) was repressed by ABA. The effect on transcription from both hormone response elements was orientation-independent, indicating that they function as inducible enhancers in their native genes.
Skriver
K
K
Carlsberg Research Laboratory Copenhagen, Denmark.
Olsen
F L
FL
Rogers
J C
JC
Mundy
J
J
D000040:Abscisic Acid / Q000494:pharmacology*; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000187:drug effects / Q000235:genetics*; D017343:Genes, Plant / Q000187:drug effects*; D005875:Gibberellins / Q000494:pharmacology*; D001467:Hordeum / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011523:Protoplasts / Q000502:physiology; D011994:Recombinant Proteins / Q000378:metabolism; D016385:TATA Box; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection; D000516:alpha-Amylases / Q000235:genetics
D005875:Gibberellins; D011994:Recombinant Proteins; D000040:Abscisic Acid; D004247:DNA; C007842:gibberellic acid; D015500:Chloramphenicol O-Acetyltransferase; D000516:alpha-Amylases