title: [Activation of human serum complement with inulin and purification of SC5b-9].
abstract: During a study of the effect of inulin on human complement, we found that complements were partially activated after incubation with inulin for one hour and completely activated after incubation for three hours at 37 degrees C. SC5b-9 was purified by DEAE-Sephacel chromatography, linear sucrose density gradients centrifugation and anti-IgM-protein A affinity chromatography. Purified SC5b-9, as assayed by immunoelectrophoresis, was a single fraction and had C5b, C6, C7/C8 alpha gamma, C8 beta, C9, C92, and S-protein bands appearing in the 10% SDS-PAGE.
authors.affiliation: Institute of Basic Medical Sciences.
authors.forename: F L
authors.initials: FL
authors.lastname: Zhou
authors.forename: D
authors.initials: D
authors.lastname: Kan
mesh_terms: D003167:Complement Activation / Q000187:drug effects*; D015938:Complement Membrane Attack Complex / Q000378:metabolism; D003165:Complement System Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D006023:Glycoproteins / Q000096:biosynthesis / Q000302:isolation & purification*; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D019096:Vitronectin
chemical_list: D015938:Complement Membrane Attack Complex; D003165:Complement System Proteins; D006023:Glycoproteins; D007328:Insulin; D008562:Membrane Glycoproteins; D019096:Vitronectin; C047610:SC5b-9 protein complex