title: Purification of NAD glycohydrolase from Neurospora crassa conidia by a polyclonal immunoadsorbent.
abstract: NAD glycohydrolase from Neurospora crassa conidia was purified by affinity chromatography on a column of polyclonal antibodies bound to an agarose matrix. The procedure was easy, non-denaturating and suitable for repetitive use of the gel. The enzyme obtained appeared homogeneous by sodiumdodecyl sulphate-polyacrylamide gel electrophoresis.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biomediche, University of Milan, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Pace
authors.forename: D
authors.initials: D
authors.lastname: Agnellini
authors.forename: G
authors.initials: G
authors.lastname: Lippoli
authors.forename: P G
authors.initials: PG
authors.lastname: Pietta
authors.forename: P L
authors.initials: PL
authors.lastname: Mauri
authors.forename: S
authors.initials: S
authors.lastname: Cinquanta
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D009699:N-Glycosyl Hydrolases / Q000276:immunology / Q000302:isolation & purification*; D009244:NAD+ Nucleosidase; D009492:Neurospora crassa / Q000032:analysis / Q000201:enzymology*; D012685:Sepharose
chemical_list: D000906:Antibodies; D012685:Sepharose; D009699:N-Glycosyl Hydrolases; D009244:NAD+ Nucleosidase