title: Purified Fc epsilon R+ bone marrow and splenic non-B, non-T cells are highly enriched in the capacity to produce IL-4 in response to immobilized IgE, IgG2a, or ionomycin.
abstract: Non-B, non-T cells from spleen and bone marrow cells produce IL-4 in response to cross-linkage of high affinity receptors for Fc epsilon R or Fc gamma RII, and to treatment with calcium ionophores. Cells bearing high affinity Fc epsilon R constituted 1 to 2% of non-B, non-T cells of spleen and of total bone marrow cells from naive donors. In mice whose immune systems had been polyclonally activated by injection with anti-IgD antibodies or had been infected with Nippostrongylus brasiliensis larvae, the frequency of Fc epsilon R+ cells in splenic non-B, non-T cells was also 1 to 2% but in bone marrow from anti-IgD-injected mice donors the frequency was approximately 5%. Cell sorting experiments revealed that all of the capacity to produce IL-4 in response to immobilized IgE or IgG2a or to ionomycin was found in the Fc epsilon R+ fraction. Among the Fc epsilon R+ spleen cells from naive donors, the frequency of IL-4-producing cells was 1/20 to 1/40 whereas in mice that had been injected with anti-IgD or infected with N. brasiliensis, the frequency of IL-4 producing cells in the Fc epsilon R+ population was approximately 1/5.
authors.affiliation: Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
authors.forename: R A
authors.initials: RA
authors.lastname: Seder
authors.forename: M
authors.initials: M
authors.lastname: Plaut
authors.forename: S
authors.initials: S
authors.lastname: Barbieri
authors.forename: J
authors.initials: J
authors.lastname: Urban
authors.forename: F D
authors.initials: FD
authors.lastname: Finkelman
authors.forename: W E
authors.initials: WE
authors.lastname: Paul
mesh_terms: D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000502:physiology*; D001853:Bone Marrow / Q000276:immunology / Q000378:metabolism*; D005260:Female; D007072:Immunoglobulin D / Q000502:physiology; D007073:Immunoglobulin E / Q000494:pharmacology*; D007074:Immunoglobulin G / Q000494:pharmacology*; D015847:Interleukin-4 / Q000096:biosynthesis*; D015759:Ionomycin / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009349:Nematode Infections / Q000276:immunology; D009559:Nippostrongylus; D011961:Receptors, Fc / Q000502:physiology*; D017455:Receptors, IgE; D013154:Spleen / Q000276:immunology / Q000378:metabolism*
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D007072:Immunoglobulin D; D007074:Immunoglobulin G; D011961:Receptors, Fc; D017455:Receptors, IgE; D015847:Interleukin-4; D007073:Immunoglobulin E; D015759:Ionomycin