title: cDNA and genomic DNA sequence of the 21.3 kDa subunit of NADH:ubiquinone reductase (complex I) from Neurospora crassa.
abstract: The primary structure of a nuclear-encoded subunit of the respiratory chain NADH:ubiquinone reductase (complex I) from Neurospora crassa was determined by sequencing cDNA, genomic DNA and the N-terminus of the protein. The sequence correlates to a protein of 200 amino acids and a molecular mass of 21349 Da. The protein is synthesized without a cleavable presequence. It contains two alpha-helices predicted to traverse the bilayer and is a constituent of the membrane part of complex I.
authors.affiliation: Institut für Biochemie, Universität Düsseldorf, F.R.G.
authors.forename: U
authors.initials: U
authors.lastname: Nehls
authors.forename: S
authors.initials: S
authors.lastname: Hemmer
authors.forename: D A
authors.initials: DA
authors.lastname: Röhlen
authors.forename: J C
authors.initials: JC
authors.lastname: Van der Pas
authors.forename: D
authors.initials: D
authors.lastname: Preis
authors.forename: U
authors.initials: U
authors.lastname: Sackmann
authors.forename: H
authors.initials: H
authors.lastname: Weiss
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016660:NAD(P)H Dehydrogenase (Quinone); D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D011808:Quinone Reductases / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D046911:Macromolecular Substances; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases