Neocarzinostatin: interaction between the antitumor-active chromophore and the carrier protein.
Conformational analysis of neocarzinostatin, an antibiotic protein with antitumor activity, in its holo-state in solution was carried out by NMR-spectroscopy. The results showed a beta-barrel structure for the carrier protein, in which the chromophore is tightly enwrapped and thus shielded from exposure to the solvent. The three-dimensional structure of this complex led to the proposal of a possible mechanism for the exposure or release of the active principle due to subtle environmental changes, e.g., in pH.
Takashima
H
H
Research Laboratories, Kuraray Co., Ltd., Okayama.
Amiya
S
S
Kobayashi
Y
Y
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011487:Protein Conformation; D009353:Zinostatin / Q000737:chemistry*
D002352:Carrier Proteins; D009353:Zinostatin