Biosynthesis of the 67 kDa high affinity laminin receptor.
High affinity interactions between cells and laminin are mediated, at least in part, by the 67 kDa laminin receptor (67 LR). A 37 kDa nascent polypeptide (37 LRP), predicted by a full length cDNA clone and obtained by in vitro translation of hybrid-selected laminin receptor mRNA, has been immunologically identified in cancer cell extracts as the putative precursor of the 67 LR. In this study, we used affinity purified antibodies developed against cDNA-deduced 37 LRP synthetic peptides in pulse chase experiments and demonstrated a precursor-product relationship between the 37 LRP and the 67 LR. Immunoblot, pulse chase and immunofluorescence experiments showed that transient transfection of the full length 37 LRP cDNA clone induced a dramatic increase in the synthesis of the 37 LRP but not of the mature 67 LR. We propose that the 67 LR results from the association of two gene products: the 37 LRP and a polypeptide yet to be identified.
Castronovo
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V
Tumor Invasion and Metastasis Section, National Cancer Institute, Bethesda, Maryland 20892.
Claysmith
A P
AP
Barker
K T
KT
Cioce
V
V
Krutzsch
H C
HC
Sobel
M E
ME
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D003001:Cloning, Molecular; D005455:Fluorescent Antibody Technique; D007797:Laminin / Q000378:metabolism; D008970:Molecular Weight; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D011971:Receptors, Immunologic / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D017474:Receptors, Laminin; D014162:Transfection
D007797:Laminin; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D017474:Receptors, Laminin