Modulation of mast cell eicosanoid synthesis by attachment.
The experiments discussed above indicate that a change in phenotype occurs when cultured mast cells attach. This modification in phenotype is associated with a downregulation of their own eicosanoid production and that of cocultured mast cells in suspension. The eicosanoid production of the floating mast cells is markedly elevated after removal from attached cells. This enhancement appears to be due to an upregulation of phospholipase activity. Experiments with actinomycin D and cycloheximide indicate that new protein synthesis is necessary for the increase to occur. This suggests that either new enzyme and/or peptides modulating phospholipase are generated.
Jakschik
B A
BA
Washington University School of Medicine, Department of Pharmacology, St. Louis, Missouri 63110.
Xu
L
L
D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000502:physiology; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002448:Cell Adhesion; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D015777:Eicosanoids / Q000096:biosynthesis*; D004789:Enzyme Activation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D007073:Immunoglobulin E / Q000276:immunology; D015289:Leukotrienes / Q000096:biosynthesis; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D010740:Phospholipases / Q000378:metabolism; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011961:Receptors, Fc / Q000502:physiology; D017455:Receptors, IgE
D000944:Antigens, Differentiation, B-Lymphocyte; D001095:Arachidonic Acids; D015777:Eicosanoids; D015289:Leukotrienes; D011961:Receptors, Fc; D017455:Receptors, IgE; D003609:Dactinomycin; D016718:Arachidonic Acid; D007073:Immunoglobulin E; D000001:Calcimycin; D003907:Dexamethasone; D003513:Cycloheximide; D001094:Arachidonate 5-Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases; D010740:Phospholipases