title: The flaA locus of Bacillus subtilis is part of a large operon coding for flagellar structures, motility functions, and an ATPase-like polypeptide.
abstract: We cloned and sequenced 8.3 kb of Bacillus subtilis DNA corresponding to the flaA locus involved in flagellar biosynthesis, motility, and chemotaxis. The DNA sequence revealed the presence of 10 complete and 2 incomplete open reading frames. Comparison of the deduced amino acid sequences to data banks showed similarities of nine of the deduced products to a number of proteins of Escherichia coli and Salmonella typhimurium for which a role in flagellar functioning has been directly demonstrated. In particular, the sequence data suggest that the flaA operon codes for the M-ring protein, components of the motor switch, and the distal part of the basal-body rod. The gene order is remarkably similar to that described for region III of the enterobacterial flagellar regulon. One of the open reading frames was translated into a protein with 48% amino acid identity to S. typhimurium FliI and 29% identity to the beta subunit of E. coli ATP synthase.
authors.affiliation: Dipartimento di Genetica e Microbiologia A. Buzzati-Traverso, Università degli Studi di Pavia, Italy.
authors.forename: A M
authors.initials: AM
authors.lastname: Albertini
authors.forename: T
authors.initials: T
authors.lastname: Caramori
authors.forename: W D
authors.initials: WD
authors.lastname: Crabb
authors.forename: F
authors.initials: F
authors.lastname: Scoffone
authors.forename: A
authors.initials: A
authors.lastname: Galizzi
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002465:Cell Movement; D003001:Cloning, Molecular; D005407:Flagella / Q000502:physiology*; D005408:Flagellin; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D009876:Operon / Q000235:genetics*; D006180:Proton-Translocating ATPases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D005408:Flagellin; C067138:flaA protein, bacteria; D006180:Proton-Translocating ATPases