Identification of a novel nuclear protein synthesized in growth-arrested human hepatoblastoma HepG2 cells.
DNA synthesis of human hepatoblastoma HepG2 cells is reversibly inhibited by butyrate. When butyrate is removed from the culture medium, cells re-enter the cell cycle, synthesizing DNA with a time lag of about 12 h. HepG2 cells, growth-inhibited for 30 h with butyrate, synthesize and accumulate a nuclear protein, called D. Protein D synthesis is inhibited in cells which, released from the butyrate block, have resumed DNA synthesis as well as in growing cells never exposed to butyrate. Protein D has been purified from growth-arrested cells and partially sequenced. The amino acid sequences of five internal trypsin peptides indicate that protein D is a novel nuclear protein.
Donadel
G
G
Institute of Biological Chemistry, University of Pisa, Italy.
Garzelli
C
C
Frank
R
R
Gabrielli
F
F
D000595:Amino Acid Sequence; D001345:Autoradiography; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D006528:Carcinoma, Hepatocellular; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007700:Kinetics; D008113:Liver Neoplasms; D008715:Methionine / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D010446:Peptide Fragments / Q000302:isolation & purification; D013462:Sulfur Radioisotopes
D002087:Butyrates; D009687:Nuclear Proteins; D010446:Peptide Fragments; D013462:Sulfur Radioisotopes; D020148:Butyric Acid; D008715:Methionine