title: Glutathione transferase pi its minimal promoter and downstream cis-acting element.
abstract: Fragments of the human glutathione S-transferase pi gene and 15 kb of its 5' flanking region have been fused to the chloramphenicol acetyl transferase (CAT) reporter gene. Transfection into a number of human cell lines (Hela, HepG2, MCF7 and EJ) has demonstrated that the AP1 binding site, located between nucleotides -58 and -65 (Cowell et al. 1988. Biochem. J. 255, 79-83), is essential for basal level promoter activity. We have also identified a positive cis-acting DNA element between nucleotides +8 and +72 which seems to be part of the promoter. No other regulatory activity was identified within the 17 kb analyzed.
authors.affiliation: Cancer Research Campaign Molecular Toxicology Research Group, University College and Middlesex School of Medicine, London, U.K.
authors.forename: C L
authors.initials: CL
authors.lastname: Xia
authors.forename: I G
authors.initials: IG
authors.lastname: Cowell
authors.forename: K H
authors.initials: KH
authors.lastname: Dixon
authors.forename: S E
authors.initials: SE
authors.lastname: Pemble
authors.forename: B
authors.initials: B
authors.lastname: Ketterer
authors.forename: J B
authors.initials: JB
authors.lastname: Taylor
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D001943:Breast Neoplasms; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005091:Exons; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D007438:Introns; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D008113:Liver Neoplasms; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection; D001749:Urinary Bladder Neoplasms
chemical_list: D004268:DNA-Binding Proteins; D007527:Isoenzymes; D015345:Oligonucleotide Probes; D016755:Proto-Oncogene Proteins c-jun; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D015500:Chloramphenicol O-Acetyltransferase; D005982:Glutathione Transferase; D011505:Protein-Tyrosine Kinases