title: Dot-enzyme-linked immunosorbent assay (Dot-ELISA) of anti-Entamoeba histolytica antibodies in human serum and colostrum.
abstract: Seroepidemiologic and clinical studies of amoebiasis require improved methods for the detection and titration of anti-Entamoeba histolytica (Eh) trophozoite antibodies. We have developed a Dot-enzyme-linked immunosorbent assay (Dot-ELISA) to titrate anti-Eh antibodies in human serum and colostrum. The anti-Eh serum IgG antibody titers from healthy individuals and from patients with amoebic liver abscess obtained, with our Dot-ELISA were similar to those obtained with a standard ELISA, but the background values were lower. Significant anti-Eh sIgA antibody titers were found in seven out of 42 colostrum samples of mothers of newborn children with diarrhea. Our Dot-ELISA is rapid, inexpensive, and might be used for the diagnosis of individuals and in seroepidemiologic studies of amoebiasis.
authors.affiliation: Departamento de Biología Celular, CINVESTAV-IPN, México, D.F.
authors.forename: R
authors.initials: R
authors.lastname: López Revilla
authors.forename: F
authors.initials: F
authors.lastname: Navarro-García
authors.forename: M
authors.initials: M
authors.lastname: Valadez-Sánchez
authors.forename: Y
authors.initials: Y
authors.lastname: López Vidal
authors.forename: J
authors.initials: J
authors.lastname: Calva Mercado
mesh_terms: D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis* / Q000097:blood; D003126:Colostrum / Q000737:chemistry*; D003968:Diarrhea, Infantile / Q000209:etiology; D004748:Entamoeba histolytica / Q000276:immunology*; D004749:Entamoebiasis / Q000150:complications / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D007074:Immunoglobulin G / Q000097:blood*; D007231:Infant, Newborn; D008101:Liver Abscess, Amebic / Q000097:blood* / Q000276:immunology; D008297:Male; D011247:Pregnancy; D011644:Puerperal Disorders / Q000175:diagnosis; D011817:Rabbits
chemical_list: D000913:Antibodies, Protozoan; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G