title: Superoxide production by Crohn's disease neutrophils.
abstract: Neutrophil superoxide anion production was measured in healthy subjects and in patients with quiescent and active Crohn's disease using superoxide dismutase inhibitable cytochrome C reduction. Three stimuli were used: phorbol 12-myristate 13-acetate (PMA1), phorbol 20-oxo-20-deoxy 12-myristate 13-acetate (PMA2), and Candida albicans in serum. Normal neutrophils produced significantly more superoxide anion than Crohn's disease neutrophils with both PMA1 (mean (SD) 9.6 (2.2) v 8.6 (1.8) nmol/10(6) cells/5 minutes, p = 0.04) and PMA2 (1.8 (0.8) v 0.8 (0.77) nmol/10(6) cells/5 minutes, p = 0.00004). With C albicans in serum, normal and Crohn's disease neutrophils produced similar amounts of superoxide anion (4.4 (1.5) v 4.3 (1.7) nmol/10(6) cells/30 minutes, not significant). Results were independent of disease activity. Superoxide anion production by PMA-stimulated Crohn's disease neutrophils is significantly lower than by normal neutrophils.
authors.affiliation: General Hospital, Birmingham.
authors.forename: F T
authors.initials: FT
authors.lastname: Curran
authors.forename: R N
authors.initials: RN
authors.lastname: Allan
authors.forename: M R
authors.initials: MR
authors.lastname: Keighley
mesh_terms: D000328:Adult; D002176:Candida albicans; D003424:Crohn Disease / Q000097:blood*; D003574:Cytochrome c Group / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology*; D013268:Stimulation, Chemical; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000008:administration & dosage / Q000494:pharmacology / Q000031:analogs & derivatives
chemical_list: C068811:20-oxo-20-deoxy-12-myristate 13-acetate; D003574:Cytochrome c Group; D013481:Superoxides; D013755:Tetradecanoylphorbol Acetate