Inhibition of gastric mucosal laminin receptor by Helicobacter pylori lipopolysaccharide: effect of nitecapone.
1. A gastric mucosal laminin receptor has been isolated from the epithelial cell membrane by affinity chromatography on Sepharose-bound laminin, and following radioiodination was incorporated into liposomes which displayed specific affinity towards the laminin-coated surface. 2. The binding of liposomal receptor to the laminin-coated surface was inhibited by Helicobacter pylori lipopolysaccharide and reached a maximum value of 96% at 50 micrograms/ml. 3. The inhibitory effect of H. pylori lipopolysaccharide on the receptor-laminin binding was prevented by an antiulcer agent, nitecapone. The effect was concentration dependent and produced a maximum response of 83% at 30 micrograms/ml of drug concentration. 4. The results demonstrate that H. pylori is capable of disrupting gastric mucosal integrity by interfering with epithelial cell-laminin binding, and that an antiucler agent, nitecapone, counteracts this effect.
Slomiany
B L
BL
Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
Piotrowski
J
J
Rajiah
G
G
Slomiany
A
A
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D002396:Catechols / Q000494:pharmacology*; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D002846:Chromatography, Affinity; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D016480:Helicobacter pylori / Q000378:metabolism*; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008070:Lipopolysaccharides; D008081:Liposomes / Q000737:chemistry; D008297:Male; D010422:Pentanones / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D011971:Receptors, Immunologic / Q000037:antagonists & inhibitors*; D017474:Receptors, Laminin
D000897:Anti-Ulcer Agents; D002396:Catechols; D007457:Iodine Radioisotopes; D008070:Lipopolysaccharides; D008081:Liposomes; D010422:Pentanones; D011971:Receptors, Immunologic; D017474:Receptors, Laminin; C057364:nitecapone