Purification and characterization of a thymocyte growth factor. 2. Biological activity of the thymocyte growth factor.
A thymocyte growth factor, THGF, purified from the supernatant of a thymic T-lymphocyte-precursor cell line (TC.SC-1/2.0), stimulated the growth of splenocytes and thymocytes that had not been activated by a mitogen. Addition of suboptimal doses of mitogen or phorbol myristate acetate did not enhance the response. The growth factor could stimulate the growth of thymocytes synergistically with interleukin 2 but its action was not mediated by interleukin 2 or by interleukin 4. L3T4-Lyt2- thymocytes, Ig- splenocytes, Ig+ splenocytes, and alpha 1-thymosin-sensitive splenocytes were targets for THGF, which also stimulated the formation of haemopoietic spleen colonies in irradiated mice.
Talaev VYu
Research Institute of Epidemiology and Microbiology, Ministry of Health of Russia, Nizhnii Novgorod.
Yarilin
A A
AA
Sharova
N I
NI
Shichkin
V P
VP
Shuvaeva
T M
TM
D000818:Animals; D002455:Cell Division; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D006133:Growth Substances / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008808:Mice, Inbred CBA; D010455:Peptides / Q000502:physiology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000737:chemistry* / Q000166:cytology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013947:Thymosin / Q000502:physiology
D003470:Culture Media; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C052275:thymocyte growth peptide; D003208:Concanavalin A; D013947:Thymosin; D013755:Tetradecanoylphorbol Acetate