title: Antigenic analysis of feline and bovine Chlamydia psittaci with monoclonal antibodies.
abstract: Monoclonal antibodies were established for antigenic analysis of feline and bovine Chlamydia psittaci. The monoclonal antibodies recognized lipopolysaccharide (LPS), 56-64, 84 or 86 kDa antigens. At least 5 antibody-binding sites were detected on LPS with the monoclonal antibodies. The 56-64 kDa antigen was suggested to have both polypeptide and carbohydrate antibody binding sites. Immunoblotting analysis of cat and cattle sera indicated that the 56-64 kDa antigen is an important antigen in host immune response. The monoclonal antibodies are extremely useful tools to analyse the structure and function of chlamydial antigens.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Matsuno
authors.forename: H
authors.initials: H
authors.lastname: Fukushi
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: K
authors.initials: K
authors.lastname: Hirai
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis*; D002371:Cat Diseases / Q000382:microbiology*; D002415:Cats; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002691:Chlamydophila psittaci / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D015151:Immunoblotting; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000276:immunology; D009956:Psittacosis / Q000382:microbiology / Q000662:veterinary*
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides