Sequences from myeloblastosis-associated virus MAV-2(O) and UR2AV involved in the formation of plaques and the induction of osteopetrosis, anemia, and ataxia.
Recombinant viruses were made between myeloblastosis-associated virus MAV-2(O) and UR2AV to examine the relationship between regions of the MAV-2(O) genome and disease induction. The env-long terminal repeat (LTR) portion of MAV-2(O), when substituted into UR2AV, was sufficient to induce osteopetrosis identical to that caused by the parent MAV-2(O). When this region was reduced to the gp37 and LTR of MAV-2(O), osteopetrosis more severe than that caused by the parent virus was induced. Recombinant viruses that contained all or part of the MAV-2(O) env gene in the absence of the MAV-2(O) LTR induced a severe, chronic anemia and late-onset osteopetrosis, leading to the conclusion that the MAV-2(O) LTR, in addition to env, was required for rapid induction of osteopetrosis. A viral recombinant, pEU, which contained the gp85 segment of UR2AV substituted into MAV-2(O), induced an ataxia/cerebellar dysfunction not seen during infection with the other chimeric or parent viruses. In vitro studies of the parent and recombinant viruses demonstrated that the ability to form plaques on chicken embryo fibroblasts correlated with the presence of the MAV-2(O) gp37 and LTR except for construct pEU. When the viruses were inoculated into 10-day-old chickens, chimeras containing the env-LTR of gp37-LTR region of MAV-2(O) induced severe regenerative anemia similar to that induced by MAV-2(O). pEU was the exception, suggesting that the unique configuration of this chimera is responsible for its unusual pathogenic properties.
Aurigemma
R E
RE
Department of Microbiology, Colorado State University, Fort Collins 80523.
Torgersen
J L
JL
Smith
R E
RE
D000740:Anemia / Q000382:microbiology*; D000818:Animals; D001259:Ataxia / Q000382:microbiology*; D001354:Avian Leukosis Virus / Q000235:genetics* / Q000472:pathogenicity; D002642:Chick Embryo; D002645:Chickens; D005347:Fibroblasts; D005814:Genes, Viral; D006394:Hemangiosarcoma / Q000382:microbiology; D007680:Kidney Neoplasms / Q000382:microbiology; D010022:Osteopetrosis / Q000382:microbiology*; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014162:Transfection; D009396:Wilms Tumor / Q000382:microbiology