title: [BsoAI--a new site specific endonuclease from Bacillus coagulans].
abstract: A new site-specific endonuclease was detected in toluene lysates of Bacillus coagulans AUCM B-732 and designated as BcoAI. The enzyme was purified by fractionation of the cell-free extract in the two-phase PEG/dextran system followed by chromatography on DEAE-sepharose and phosphocellulose and shown to be free of nonspecific nucleases and phosphatases. BcoAI has three cleavage sites on lambda DNA, but does not cleave SV40, pBR322 and pUC19 DNA. BcoAI recognizes the sequence 5' CAC decreases GTG 3' on double-stranded DNA and cleaves it as indicated by the arrow to yield blunt-ended DNA fragments. Thus, BcoAI is a true isoschizomer of PmaCI from Pseudomonas maltophila C.
authors.forename: N N
authors.initials: NN
authors.lastname: Sokolov
authors.forename: M A
authors.initials: MA
authors.lastname: El'darov
authors.forename: N V
authors.initials: NV
authors.lastname: Anikeĭcheva
authors.forename: I V
authors.initials: IV
authors.lastname: Karpychev
authors.forename: O T
authors.initials: OT
authors.lastname: Samko
authors.forename: A B
authors.initials: AB
authors.lastname: Fitsner
authors.forename: A A
authors.initials: AA
authors.lastname: Kalugin
authors.forename: E B
authors.initials: EB
authors.lastname: Khoroshutina
authors.forename: K G
authors.initials: KG
authors.lastname: Skriabin
mesh_terms: D001345:Autoradiography; D001407:Bacillus / Q000201:enzymology*; D010582:Bacteriophage lambda / Q000378:metabolism; D002848:Chromatography, DEAE-Cellulose; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D016297:Mutagenesis, Site-Directed
chemical_list: D004279:DNA, Viral; C048066:endodeoxyribonuclease PmaCI; D015252:Deoxyribonucleases, Type II Site-Specific
vernacular_title: BcoAI--novaia saĭt-spetsificheskaia éndonukleaza iz Bacillus coagulans.