Amphotericin B stimulates secretion of beta-hexosaminidase from mouse adherent spleen cells.
Secretion of the lysosomal enzyme hexosaminidase is induced by amphotericin B in mouse spleen adherent cells that show a significant increase in their candidacidal activity. The stimulation of beta-hexosaminidase is both time and dose dependent. Amphotericin B treatment did not change hexosaminidase expression that is represented mainly by "A-type" hexosaminidase in macrophages, on the basis of its biochemical properties.
Beccari
T
T
Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università di Perugia, Italy.
Mazzolla
R
R
Constanzi
E
E
Datti
A
A
Barluzzi
R
R
Bistoni
F
F
Orlacchio
A
A
D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000378:metabolism; D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D002848:Chromatography, DEAE-Cellulose; D006923:Hymecromone / Q000031:analogs & derivatives / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology; D051379:Mice; D008970:Molecular Weight; D013154:Spleen / Q000737:chemistry / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D013696:Temperature; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism*
C025158:4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside; D006923:Hymecromone; D000666:Amphotericin B; D001619:beta-N-Acetylhexosaminidases; D000117:Acetylglucosamine