Analysis of nucleic acids by ion-spray mass spectrometry.
We analyzed various synthetic oligonucleotides with the use of ion-spray mass spectrometer. The most suitable conditions were investigated for analyzing oligonucleotides with molecular weights ranging from 3000 to 15000. We have found that the pH of the solvent was critical to reduce the formation of adduct ions in the mass spectra. Tandem mass spectrometry was also used to study the sequences.
Kawase
Y
Y
Biotechnology Research Laboratories, Takara Shuzo Co. Ltd., Shiga, Japan.
Umeda
Y
Y
Kato
I
I
D001483:Base Sequence; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009841:Oligonucleotides / Q000032:analysis* / Q000138:chemical synthesis
D009841:Oligonucleotides