title: Testosterone and corticosterone co-regulate messenger RNA coding for secretory proteins in the epididymis of the lizard (Lacerta vivipara).
abstract: The hormonal requirements for the regulation of Lv132 mRNA coding for two proteins secreted by the principal cells of the lizard epididymis were examined by organotypic culture experiments. Testosterone, R1881 and corticosterone induced accumulation of Lv132 mRNA in explants from lizards castrated immediately after differentiation of the principal cells. The induction by testosterone was inhibited by the addition of cyproterone acetate. Progesterone and oestradiol alone or in presence of testosterone were ineffective. Unlike the induction by testosterone, the effect of corticosterone did not require binding on the androgen receptor as shown by competition binding studies. Corticosterone failed to induce gene expression in organs containing only reserve cells in their epithelium at the onset of the culture. However, corticosterone plus testosterone had a synergistic effect. These data suggest that testosterone promotes the differentiation of principal cells from reserve cells during the culture time and that a primary action of testosterone is necessary to confer corticosterone responsiveness on this tissue. Furthermore, the primary effects of testosterone could be memorized by the tissue because the corticosterone responsiveness persists after castration.
authors.affiliation: Biologie cellulaire, Université Blaise Pascal, Aubière, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Courty
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D003345:Corticosterone / Q000502:physiology*; D003534:Cyproterone / Q000031:analogs & derivatives / Q000494:pharmacology; D017373:Cyproterone Acetate; D004357:Drug Synergism; D004822:Epididymis / Q000737:chemistry / Q000502:physiology*; D005786:Gene Expression Regulation; D015151:Immunoblotting; D008116:Lizards / Q000502:physiology*; D008297:Male; D009924:Organ Culture Techniques; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000502:physiology*; D011944:Receptors, Androgen / Q000032:analysis; D013739:Testosterone / Q000502:physiology*
chemical_list: D011506:Proteins; D012333:RNA, Messenger; D011944:Receptors, Androgen; D013739:Testosterone; D017373:Cyproterone Acetate; D003534:Cyproterone; D003345:Corticosterone