Soluble CD23 displays T-cell growth enhancing activity.
Soluble CD23 (sCD23) enhances, in a dose-dependent manner, the number of secondary T-cell colonies generated by peripheral blood-derived agar T-colony cells in the presence of phytohaemagglutinin (PHA) and interleukin-2 (IL-2). This effect is not affected by IL-1 or IL-4 but is abolished by an anti-CD23 monoclonal antibody (mAb) or by IgE. No colonies were observed when sCD23 was added to PHA- or IL-2-free cultures. sCD23 also enhanced the cloning frequency of primary T-colony cells in a limiting dilution assay. These data provide the first direct evidence that sCD23 recruits T-cell clones in peripheral blood-born T cells and may be involved indirectly in the regulation of IgE response.
Lecron
J C
JC
URA C.N.R.S. 1338, Hôpital La Milétrie, Poitiers, France.
Morel
F
F
Tanzer
J
J
Gombert
J
J
Goube de Laforest
P
P
D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology; D010835:Phytohemagglutinins / Q000276:immunology; D011961:Receptors, Fc / Q000276:immunology*; D017455:Receptors, IgE; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000944:Antigens, Differentiation, B-Lymphocyte; D007376:Interleukin-2; D010835:Phytohemagglutinins; D011961:Receptors, Fc; D017455:Receptors, IgE; D011994:Recombinant Proteins