Enzyme-linked immunosorbent assay (ELISA) for measles antibody. A comparison with haemagglutination inhibition, immunofluorescence and plaque neutralization tests.
An enzyme-linked immunosorbent assay (ELISA) for measles antibodies was compared with Plaque Neutralization (PRN), Haemagglutination inhibition (HI) and Fluorescent antibody (IFA) tests in 181 sera from vaccinated children and umbilical cord. Of 179 positive samples by the sensitive PRN, only two, with titers of 8, were negative by ELISA (copositivity of 98.9%). IFA and HI presented, respectively, copositivities of 93.3% and 82.7%. The ELISA presented a high sensitivity as well as a good reproducibility and represents an alternative for the time consuming PRN for detection of low measles antibodies.
de Souza
V A
VA
Instituto de Medicina Tropical de São Paulo, (LIM-52-HC-FMUSP), Brazil.
Pannuti
C S
CS
Sumita
L M
LM
Albrecht
P
P
D000914:Antibodies, Viral / Q000097:blood*; D002648:Child; D015941:Complement Hemolytic Activity Assay; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D008459:Measles virus / Q000276:immunology*; D009500:Neutralization Tests; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral