Enhanced production of interferon-gamma by T lymphocytes cloned from rejected kidney grafts.
Seventy-seven T cell clones were generated from cell blasts infiltrating rejected kidney allografts. All clones, either CD4 or CD8, displayed cytolytic activity evaluated by lectin-dependent cell-mediated cytotoxicity (LDCC) and natural killer activities. Furthermore, both types of clones were able to produce IFN-gamma following PHA stimulation. These data suggest that the graft infiltrate is characterized by T cell clones with cytolytic potential responsible for the killing of graft cells. The production of IFN-gamma, enhancing the class II MHC expression, may amplify the recipient immune response.
Benvenuto
R
R
Fondazione Andrea Cesalpino, I Clinica Medica, II Patologia Chirurgica, Università La Sapienza, Roma, Italy.
Bachetoni
A
A
Cinti
P
P
Sallusto
F
F
Franco
A
A
Molajoni
E R
ER
Barnaba
V
V
Balsano
F
F
Cortesini
R
R
D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D017252:CD3 Complex; D016827:CD8 Antigens; D002999:Clone Cells; D006084:Graft Rejection; D006801:Humans; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000096:biosynthesis*; D016030:Kidney Transplantation / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D016827:CD8 Antigens; D011948:Receptors, Antigen, T-Cell; D007371:Interferon-gamma