Characterization of a candidate gene for GP72, an insect stage-specific antigen of Trypanosoma cruzi.
The Trypanosoma cruzi insect stage-specific antigen GP72 was purified from epimastigotes and the amino acid sequences of peptide fragments determined. Oligonucleotides derived from these data were used to amplify and clone a cDNA sequence, which was used to isolate a full-length gene. All the sequenced peptides were encoded within the gene. The characteristics of the encoded 62,600-Da protein, including a potential amino-terminal signal sequence, a hydrophobic carboxy-terminus, and a large number of potential O-glycosylation sites, are consistent with the properties of GP72. No sequence homologies were found in searches of DNA and protein data banks. GP72 is encoded by a single pair of non-telomeric allelic genes.
Cooper
R
R
Laboratory of Molecular Parasitology, Rockefeller University, New York, NY 10021.
Inverso
J A
JA
Espinosa
M
M
Nogueira
N
N
Cross
G A
GA
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D016054:DNA, Protozoan / Q000235:genetics; D005796:Genes; D006023:Glycoproteins / Q000235:genetics* / Q000302:isolation & purification; D007303:Insect Vectors / Q000469:parasitology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010750:Phosphoproteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics*; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000254:growth & development / Q000276:immunology
D000953:Antigens, Protozoan; D016054:DNA, Protozoan; C038790:GP72 antigen, Trypanosoma; D006023:Glycoproteins; D010750:Phosphoproteins; D015800:Protozoan Proteins