NMR investigation of the interaction of mithramycin A with d(ACCCGGGT)2.
The binding of mithramycin A to d(ACCCGGGT)2 has been investigated by one- and two-dimensional 1H NMR spectroscopy. Titration of the drug into the octamer solution results in loss of the oligonucleotide C2 symmetry at stoichiometric ratios less than 4 drug molecules per duplex. However, at a ratio of 4:1 (drug/duplex), the C2 symmetry of the oligonucleotide is restored. From these data it is evident that more than one complex forms at ratios less than 4:1 while only one complex predominates at the ratio 4:1. This is the first report of a DNA octamer which binds 4 large drug molecules. These results are compared to those we have recently reported for mithramycin binding to d(ATGCAT)2, where only a single, bound complex is observed, with a stoichiometry of 2:1.
Keniry
M A
MA
Research School of Chemistry, Australian National University, Canberra.
Banville
D L
DL
Levenson
C
C
Shafer
R H
RH
D001483:Base Sequence; D001665:Binding Sites; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D008926:Plicamycin / Q000031:analogs & derivatives* / Q000737:chemistry
D009838:Oligodeoxyribonucleotides; C066851:mithramycin A; D008926:Plicamycin