title: Modulatory role of angiotensin-II in the secretion of atrial natriuretic factor in rabbits.
abstract: This study was designed to investigate whether the increase in circulating atrial natriuretic factor (ANF) levels produced by angiotensin II (Ang II) is a consequence of the hemodynamic changes or whether it occurs also in the absence of pressor changes. For this purpose in anesthetized and awake rabbits we evaluated the effects of Ang II (0.1 micrograms/kg.min) alone or during the simultaneous infusion of sodium nitroprusside (NP) at a dose titrated to abolish the pressor effects. Systemic blood pressure increased from 76 +/- 4 to 113 +/- 5 mm Hg (P less than 0.001) during Ang II and from 76 +/- 2 to 75 +/- 3 mm Hg (P = NS) during Ang II plus NP. The alpha-adrenergic agonist phenylephrine, used as a control, raised blood pressure from 65 +/- 2 to 101 +/- 8 mm Hg (P less than 0.001), and its pressor effect was abolished by the concomitant infusion of NP (64 +/- 2 to 61 +/- 1 mm Hg; P = NS). The increase in plasma ANF levels produced by Ang II alone (from 36.5 +/- 5 to 237 +/- 57 pg/ml; P less than 0.001) was not different from that observed during Ang II plus NP (from 46 +/- 10 to 207 +/- 88 pg/ml; P less than 0.001). In contrast, the stimulatory effect on ANF release of phenylephrine (from 56.1 +/- 9 to 202 +/- 40 pg/ml; P less than 0.001) was completely abolished when its pressor effects were prevented by the combined infusion of NP (from 58.5 +/- 15 to 42.3 +/- 10 pg/ml; P = NS). These results show that the stimulatory effect of Ang II on ANF release can be clearly dissociated from its pressor effect, whereas the increase in plasma ANF levels caused by phenylephrine is strictly related to its hemodynamic effect. Therefore, Ang II is capable of modulating ANF secretion in a manner that is independent of its pressor actions. In addition, our results suggest that ANF release is not solely linked to myocyte stretch.
authors.affiliation: 1. Clinica Medica, Università degli Studi di Napoli, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Volpe
authors.forename: P
authors.initials: P
authors.lastname: Pepino
authors.forename: G
authors.initials: G
authors.lastname: Lembo
authors.forename: S
authors.initials: S
authors.lastname: Pignalosa
authors.forename: A F
authors.initials: AF
authors.lastname: Mele
authors.forename: S
authors.initials: S
authors.lastname: Rubattu
authors.forename: G
authors.initials: G
authors.lastname: Condorelli
authors.forename: E
authors.initials: E
authors.lastname: Covino
authors.forename: B
authors.initials: B
authors.lastname: Trimarco
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology / Q000502:physiology*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood / Q000378:metabolism*; D001794:Blood Pressure / Q000187:drug effects; D008297:Male; D009599:Nitroprusside / Q000494:pharmacology; D009994:Osmolar Concentration; D010656:Phenylephrine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D011817:Rabbits
chemical_list: D000804:Angiotensin II; D009599:Nitroprusside; D010656:Phenylephrine; D009320:Atrial Natriuretic Factor