Identification of a fibroblast growth factor-binding protein in Drosophila melanogaster.
As assessed by competitive binding and protein-crosslinking experiments, Drosophila melanogaster cells possess basic fibroblast growth factor (bFGF)-specific binding proteins that are similar to FGF receptors on vertebrate cells in molecular weight and binding affinity; these D. melanogaster cells, however, have no detectable binding proteins for acidic fibroblast growth factor (aFGF). Consistent with the presence of bFGF-specific binding proteins, D. melanogaster cells degrade bFGF but not aFGF. These results indicate the conservation of heparin-binding growth factors and receptors between vertebrates and D. melanogaster.
Doctor
J S
JS
McArdle Laboratory for Cancer Research, Department of Oncology, University of Wisconsin, Madison 53706.
Hoffmann
F M
FM
Olwin
B B
BB
D000818:Animals; D001667:Binding, Competitive; D004331:Drosophila melanogaster / Q000378:metabolism*; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D008970:Molecular Weight; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017468:Receptors, Fibroblast Growth Factor
D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D005346:Fibroblast Growth Factors