Single mid-chain GlcNAc beta 1-6Gal beta 1-4R sequences of linear oligosaccharides are resistant to endo-beta-galactosidase of Bacteroides fragilis.
Endo-beta-galactosidase (EC 3.2.1.103) of Bacteroides fragilis, at 250 mU ml-1, did not cleave the internal galactosidic linkage of the linear radiolabelled trisaccharide GlcNAc beta 1-6Gal beta 1-4GlcNAc, or those of tetrasaccharides Gal beta 1-4GlcNAc beta 1-6Gal beta 1-4GlcNAc and Gal beta 1-4GlcNAc beta 1-6Gal beta 1-4Glc. The isomeric glycans which contained the GlcNAc beta 1-3Gal beta 1-4GlcNAc/Glc sequence were readily cleaved.
Renkonen
O
O
Institute of Biotechnology, University of Helsinki, Finland.
Penttilä
L
L
Niemelä
R
R
Leppänen
A
A
D001441:Bacteroides fragilis / Q000201:enzymology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002854:Chromatography, Paper; D006026:Glycoside Hydrolases; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity; D001616:beta-Galactosidase / Q000378:metabolism*
D009844:Oligosaccharides; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase