title: Heterogeneity amongst fibroblasts in the production of migration stimulating factor (MSF): implications for cancer pathogenesis.
abstract: Fetal skin fibroblasts migrate into 3D collagen gels to a significantly greater extent than do adult cells. This enhanced motility of fetal fibroblasts appears to result from the production of a "migration stimulating factor" (MSF) which is not made by their normal adult counterparts. Adult skin fibroblasts retain responsiveness to MSF and cells exposed to this factor achieve the elevated levels of migration characteristic of fetal cells. MSF has been purified to homogeneity, has an apparent molecular mass of 70 kD and has been further characterized in terms of a number of biochemical parameters. Studies concerned with the mechanism of action of MSF indicate that it stimulates the production of a high molecular weight class of hyaluronic acid (HA). Concurrent exposure of cells to Streptomyces hyaluronidase blocks the stimulation of adult fibroblast migration by MSF. In a related series of experiments, we have shown that TGF-beta inhibits the effects of MSF on both cell migration and HA production. Taken together, these data suggest that the stimulation of fibroblast migration by MSF is dependent upon (and may directly result from) a primary induction of HA synthesis. We have previously reported that skin fibroblasts obtained from patients with sporadic and familial breast cancer, as well as the unaffected first-degree relatives of familial breast cancer patients, commonly display a fetal-like migratory phenotype. Subsequent work has indicated that (a) these fetal-like cells also produce MSF, and (b) detectable levels of MSF are present in the serum of sporadic breast cancer patients both prior to and following surgical resection of the primary tumor mass. On the basis of these and related observations, we have put forward an hypothesis suggesting that the disruption in normal epithelial-mesenchymal interactions caused by the persistent production of MSF by fibroblasts in the adult may contribute directly to the pathogenesis of an epithelial cancer. The demonstration of aberrant fibroblasts in sporadic cancer patients (both in our own and independent studies) is not consistent with the "germ-line genetic lesion" model commonly invoked to account for the presence of such cells in patients with hereditary cancer syndromes.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Department of Cell and Structural Biology, University of Manchester, England.
authors.forename: S L
authors.initials: SL
authors.lastname: Schor
authors.forename: A M
authors.initials: AM
authors.lastname: Grey
authors.forename: M
authors.initials: M
authors.lastname: Picardo
authors.forename: A M
authors.initials: AM
authors.lastname: Schor
authors.forename: A
authors.initials: A
authors.lastname: Howell
authors.forename: I
authors.initials: I
authors.lastname: Ellis
authors.forename: G
authors.initials: G
authors.lastname: Rushton
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000503:physiopathology; D003238:Connective Tissue / Q000502:physiology; D016207:Cytokines / Q000096:biosynthesis / Q000494:pharmacology / Q000502:physiology*; D005260:Female; D005333:Fetus; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D005353:Fibronectins; D006801:Humans; D009369:Neoplasms / Q000209:etiology / Q000503:physiopathology*
chemical_list: D016207:Cytokines; C067625:FN1 protein, human; D005353:Fibronectins