title: Surveys of human enterotoxigenic Escherichia coli from three different geographical areas for possible colonization factors.
abstract: Enterotoxigenic Escherichia coli (ETEC) from Burma, central Africa (Rwanda and Zaire) and Peru, were screened by enzyme-linked immunoassays for the colonization factor antigens (CFAs) and putative colonization factors (PCFs): CFA/I, CFA/II, which consists of three coli surface-associated (CS) antigens, CS1, CS2 and CS3, CFA/III, CFA/IV (CS4, CS5, CS6), CS7, PCFO9, PCFO159. H4, PCFO166, and CS17. The highest proportion of ETEC with identifiable colonization factors (71%) were found in the strains from Burma, which were mainly positive for CFA/I (38%), but strains producing CFA/II (4%), CFA/IV (11%), CS7 (10%), CS17 (4%), PCFO159, H4 (2%) and PCFO166 (2%) were also found. Sixty-nine percent of the ETEC from central Africa were positive for known colonization factors. While CFA/I positive strains were important (12%), a higher number of ETEC producing CFA/IV (33%) and CS17 (24%) were found. Fifty-two percent of the Peruvian strains produced identifiable colonization factors. The largest group of strains produced antigens of the CFA/IV complex (17%), while ETEC producing CFA/II (6%), CFA/III and CS6 (2%), CS7 (6%), PCFO9 (6%), PCFO166 (8%) and CS17 (7%) were also found. These surveys show that there is a considerable variation in the proportions and types of colonization factor found in different geographical areas. From 29 to 48% of the ETEC did not possess an identifiable colonization factor. These were particularly of the LT only producing type. These results have important implications for vaccine formulation.
authors.affiliation: Division of Enteric Pathogens, Central Public Health Laboratory, Colindale, London.
authors.forename: M M
authors.initials: MM
authors.lastname: McConnell
authors.forename: M L
authors.initials: ML
authors.lastname: Hibberd
authors.forename: M E
authors.initials: ME
authors.lastname: Penny
authors.forename: S M
authors.initials: SM
authors.lastname: Scotland
authors.forename: T
authors.initials: T
authors.lastname: Cheasty
authors.forename: B
authors.initials: B
authors.lastname: Rowe
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis*; D015023:Democratic Republic of the Congo; D003968:Diarrhea, Infantile / Q000382:microbiology; D004768:Enterotoxins / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000145:classification / Q000276:immunology* / Q000378:metabolism; D004927:Escherichia coli Infections / Q000382:microbiology; D036781:Fimbriae Proteins; D006801:Humans; D007223:Infant; D002052:Myanmar; D010568:Peru; D012432:Rwanda; D012703:Serotyping
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004768:Enterotoxins; C022924:colonization factor antigens; D036781:Fimbriae Proteins