Induction of CD8+ suppressor T cells by treatment with DMBA and TPA in vitro.
Antigen-nonspecific CD8+ T suppressor cells, which suppressed delayed-type hypersensitivity (DTH) against sheep red blood cells in BALB/c mice, were induced by incubating spleen cells from mice treated with 7,12-dimethylbenz[a]anthracene (DMBA), a tumor initiator, with 12-O-tetradecanoylphorbol 13-acetate (TPA), a tumor promoter. The optimal condition was incubation in 3.2 x 10(-8) mol/5 ml of TPA for 4 days. It was shown that induction of the suppressor cells required macrophages from mice treated with DMBA. These data were consistent with the results of previous work, in which CD8+ suppressor cells were induced by painting BALB/c mice with TPA following DMBA treatment. DTH was suppressed in the culture supernatants of spleen cells from mice treated with DMBA and TPA; the suppression was genetically unrestricted. The suppressor factor was resistant to trypsin and sensitive to heating at 56 degrees C for 30 min and had affinity for the macrophages.
Negoro
T
T
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Iinuma
F
F
Watanabe
M
M
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology*; D000818:Animals; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis*; D004305:Dose-Response Relationship, Drug; D006968:Hypersensitivity, Delayed; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects; D008285:Major Histocompatibility Complex; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013491:Suppressor Factors, Immunologic / Q000032:analysis; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D015704:CD4 Antigens; D016827:CD8 Antigens; D013491:Suppressor Factors, Immunologic; D015127:9,10-Dimethyl-1,2-benzanthracene; D013755:Tetradecanoylphorbol Acetate