Paraquat depresses the activity of angiotensin converting enzyme expressed by human umbilical vein endothelial cells in culture.
The activity of angiotensin converting enzyme (ACE) in cell lysate of cultured human umbilical vein endothelial cells (HUVEC) after a 24-hour incubation with 10(-3) and 10(-4)M of paraquat (PQ) was decreased. However, LDH released into the culture medium of HUVEC during the 24-hour incubation with PQ was not increased. Many investigators show that the change in serum ACE activity reflects the impairment of vascular endothelial cells. We showed in this report that ACE was decreased even at an early stage of endothelial injury induced by PQ, when LDH release is not yet increased.
Watanabe
K
K
Second Department of Internal Medicine, School of Medicine, Fukuoka University, Japan.
Tamaru
N
N
Yoshida
M
M
D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010269:Paraquat / Q000494:pharmacology*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D014471:Umbilical Veins
D007770:L-Lactate Dehydrogenase; D007703:Peptidyl-Dipeptidase A; D010269:Paraquat