Analysis of primary T cell responses to intact and fractionated microbial pathogens.
Freshly isolated human T lymphocytes were tested for their response to mycobacteria, mycobacterial lysates, 2 dimensional (2D) PAGE separated mycobacterial lysates, leishmania and defined leishmanial antigen preparations. While gamma delta T cells proliferated vigorously in the presence of mycobacteria and mycobacteria derived lysates, a significant stimulation from 2 D gel separated lysates was not detected. In addition gamma delta T cells failed to respond towards leishmania or leishmanial components. In the alpha beta T cell compartment some donors, presumably according to their state of immunity against mycobacteria, responded to mycobacteria, mycobacterial lysates and 2 D gel separated mycobacterial lysates. Neither freshly isolated gamma delta T cells nor alpha beta T cells from naive donors did mount a significant immune response against leishmania.
Pfeffer
K
K
Institute of Medical Microbiology and Hygiene, Technical University of Munich, FRG.
Schoel
B
B
Gulle
H
H
Moll
H
H
Kromer
S
S
Kaufmann
S H
SH
Wagner
H
H
D000818:Animals; D006360:Heat-Shock Proteins / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D007891:Leishmania / Q000276:immunology; D008213:Lymphocyte Activation; D009161:Mycobacterium / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D006360:Heat-Shock Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta