[Study of the calmodulin-dependent regulation of calcium adenosine triphosphatase of erythrocyte membranes in patients with ischemic heart disease].
The inhibitor calmodulin (R 24571) was examined for effects on the activity of red blood cell Ca-ATPases in patients with coronary heart disease during the treatment with nitrates, beta-blockers and calcium antagonists. The maximum activity of Ca-ATPase was measured in the erythrocytes perforated with saponine in the presence of endogenous regulators at a concentration of Ca2+ of 3-5 microM. Patients with high and low Ca-ATPase activity were identified. In the control group R24571 failed to affect Ca-ATPase activity. In patients, the calmodulin inhibitor caused both Ca-ATPase activation and inhibition. The effects of R 24571 correlated with the severity of the patients' condition. In effective therapy, the action of the calmodulin inhibitor became lower on Ca-ATPase activity. It was concluded that there was Ca-ATPase regulation imbalance in patients with coronary heart diseases.
Malaia
L T
LT
Petruniaka
V V
VV
Rudyk
Iu S
IuS
D000328:Adult; D000368:Aged; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000097:blood*; D002147:Calmodulin / Q000037:antagonists & inhibitors / Q000097:blood*; D003327:Coronary Disease / Q000097:blood*; D003470:Culture Media; D003864:Depression, Chemical; D004789:Enzyme Activation / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D012720:Severity of Illness Index
D002147:Calmodulin; D003470:Culture Media; D007093:Imidazoles; C031938:calmidazolium; D000252:Calcium-Transporting ATPases
Issledovanie kal'modulinzavisimoĭ reguliatsii Ca-ATFazy membran éritrotsitov bol'nykh ishemicheskoĭ bolezn'iu serdtsa.