Characteristics of N-acetylglucosamine transfer to nuclear acceptors of rat hepatocytes.
In this report, we describe the main characteristics of the transfer of N-acetylglucosamine within the nucleus of rat hepatocytes. The glycosylation pathway includes the presence of lipids which mediate the nuclear proteins glycosylation. The level of dolichylphosphate seems low and thus could be a regulation factor in the nuclear glycosylations. The discussion deals with the membranous character of the acceptors and the N-acetylglucosaminyltransferase, the N-linkage of the sugar moiety to nuclear proteins and the function of such glycosylation.
Letoublon
R
R
Laboratoire de Biochimie des Membranes (LBTM-CNRS UM 380024) Université Claude Bernard Lyon 1, Villeurbanne, France.
Merit
X
X
Frot-Coutaz
J
J
D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D001414:Bacitracin / Q000494:pharmacology; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism*; D004288:Dolichol Phosphates / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism; D006031:Glycosylation; D007700:Kinetics; D050356:Lipid Metabolism; D008099:Liver / Q000166:cytology; D017351:N-Acetylglucosaminyltransferases; D009685:Nuclear Envelope / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014415:Tunicamycin / Q000494:pharmacology
D004288:Dolichol Phosphates; D009687:Nuclear Proteins; D014415:Tunicamycin; C028306:dolichol monophosphate; D001414:Bacitracin; D005964:Glucosyltransferases; D017351:N-Acetylglucosaminyltransferases; C037649:N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase; D000117:Acetylglucosamine