title: [Stability of human bone cortex following various preservation and sterilization methods].
abstract: In a biomechanical study we evaluated the stability of human femurs. In order to document the inter- and intraindividual differences in bending stability, and the changes in breaking strength after different storing and sterilizing procedures, we tested 200 bony blocks, which were harvested from the femurs of 5 multiorgan donors. The bending tests were performed with a material testing machine (ZWICK) under standardized conditions. The bone blocks we randomized into five different treatment groups (-25 degrees C deep freezing, steam sterilization, lyophilization, lyophilization + gamma irradiation, lyophilization + gamma-irradiation in argon). The statistical analysis was performed by a multivariant analysis. Our results revealed significant differences concerning breaking strength among different femurs of the 5 multiorgan donors, and a significant difference concerning stability in different areas of the same femur. Further on we found a significant difference with regard to the treatment modalities of the five groups. Compared to the control group (-25 degrees C deep freezing), we documented a decrease of 8.7% after steam sterilization, an increase of 18.9% after lyophilization, a decrease of 1.7% after lyophilization + gamma-irradiation, and an increase of 3.4% after lyophilization + gamma-irradiation in argon.
authors.affiliation: Heinrich-Heine Universität Düsseldorf, Orthopädische Klinik und Poliklinik.
authors.forename: J
authors.initials: J
authors.lastname: Jerosch
authors.forename: H
authors.initials: H
authors.lastname: Muchow
authors.forename: H
authors.initials: H
authors.lastname: Clahsen
mesh_terms: D000328:Adult; D001696:Biomechanical Phenomena; D016025:Bone Transplantation; D005269:Femur / Q000502:physiology* / Q000528:radiation effects; D005612:Freeze Drying; D005615:Freezing; D006801:Humans; D008297:Male; D008875:Middle Aged; D011309:Preservation, Biological / Q000379:methods*; D013227:Steam; D013242:Sterilization; D013718:Tensile Strength
chemical_list: D013227:Steam
vernacular_title: Uber die Stabilität von humaner Kortikalis nach verschiedenen Konservierungs- und Sterilisationsverfahren.