Insulin-like growth factor I (IGF-I) from cow colostrum: purification and characterization.
Insulin-like growth factor I (IGF-I) has been purified from defatted cow colostrum. The purification procedure involved cation exchange chromatography on CM Affigel blue, acid gel-filtration and two HPLCs. This purification process constitutes a significant improvement, in terms of yield and rapidity, over the previously reported procedures. Purified IGF-I was found to be 95% pure by N-terminal amino acid sequence analysis. After isolation of peptides from a digest of IGF-I obtained using staphylococcus aureus protease, the complete sequence was established and found to be identical to that of bovine or human plasma IGF-I.
Marcotty
C
C
Faculté de Médecine, Université de Liège, Belgique.
Frankenne
F
F
Van Beeumen
J
J
Maghuin-Rogister
G
G
Hennen
G
G
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003126:Colostrum / Q000737:chemistry*; D005260:Female; D007334:Insulin-Like Growth Factor I / Q000737:chemistry / Q000302:isolation & purification*
D007334:Insulin-Like Growth Factor I