A guide to electron paramagnetic resonance spectroscopy of Photosystem II membranes.
This guide is intended to aid in the detection and identification of paramagnetic species in Photosystem II membranes, by electron paramagnetic resonance spectroscopy. The spectral features and occurrence of each of the electron paramagnetic resonance signals from Photosystem II are discussed, in relation to the nature of the moiety giving rise to the signal and the role of that species in photosynthetic electron transport. Examples of most of the signals discussed are shown. The electron paramagnetic resonance signals produced by the cytochrome b6f and Photosystem I complexes, as well as the signals from other common contaminants, are also reviewed. Furthermore, references to seminal experiments on bacterial reaction centers are included. By reviewing both the spectroscopic and biochemical bases for the electron paramagnetic resonance signals of the cofactors that mediate photosynthetic electron transport, this paper provides an introduction to the use and interpretation of electron paramagnetic resonance spectroscopy in the study of Photosystem II.
Miller
A F
AF
Department of Biochemistry, Brandeis University, Waltham, MA 02254.
Brudvig
G W
GW
D002462:Cell Membrane / Q000378:metabolism; D000458:Cyanobacteria / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D010944:Plants / Q000378:metabolism*
D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex