Crystal-induced neutrophil activation. I. Initiation and modulation of calcium mobilization and superoxide production by microcrystals.
The effects of monosodium urate and calcium pyrophosphate dihydrate crystals on the levels of cytoplasmic free calcium and on the oxidative burst in normal human blood neutrophils were examined. The pattern of sensitivity to granulocyte-macrophage colony-stimulating factor, colchicine, cytochalasin B, pertussis toxin, diglyceride kinase, and protein kinase C inhibitors differentiated the mechanism(s) of neutrophil activation by the crystals from that involved in the responses to soluble chemotactic factors and indicated that individual crystals can use several activation pathways.
Naccache
P H
PH
Départment de Médicine, Université Laval, Ste Foy, Quebec, Canada.
Grimard
M
M
Roberge
C J
CJ
Gilbert
C
C
Lussier
A
A
de Médicis
R
R
Poubelle
P E
PE
D000470:Alkaloids / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002131:Calcium Pyrophosphate / Q000494:pharmacology*; D002227:Carbazoles / Q000494:pharmacology; D002772:Cholera Toxin / Q000494:pharmacology; D003078:Colchicine / Q000494:pharmacology; D003460:Crystallization; D003571:Cytochalasin B / Q000494:pharmacology; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D019852:Diacylglycerol Kinase; D004347:Drug Interactions; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D026121:Indole Alkaloids; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D037342:Pertussis Toxin; D010770:Phosphotransferases / Q000037:antagonists & inhibitors; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011744:Pyrimidinones / Q000494:pharmacology; D019311:Staurosporine; D013481:Superoxides / Q000378:metabolism*; D013844:Thiazoles / Q000494:pharmacology; D014527:Uric Acid / Q000494:pharmacology*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D000470:Alkaloids; D002227:Carbazoles; D026121:Indole Alkaloids; D011744:Pyrimidinones; D013844:Thiazoles; D010566:Virulence Factors, Bordetella; D013481:Superoxides; D014527:Uric Acid; D003571:Cytochalasin B; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D002772:Cholera Toxin; C047505:R 59022; C049985:staurosporine aglycone; D037342:Pertussis Toxin; D010770:Phosphotransferases; D019852:Diacylglycerol Kinase; D011493:Protein Kinase C; D019311:Staurosporine; D003078:Colchicine; D002118:Calcium; D002131:Calcium Pyrophosphate