Assignment of the human granulocyte colony-stimulating factor receptor gene (CSF3R) to chromosome 1 at region p35-p34.3.
The gene for the granulocyte colony-stimulating factor (G-CSF) receptor (CSF3R) was localized on the p35-p34.3 region of human chromosome 1 by in situ hybridization using human G-CSF receptor cDNA as the probe. Polymerase chain reaction using oligonucleotides specific for the human CSF3R produced a specifically amplified DNA fragment with DNA from mouse A9 cells that contained human chromosome 1 but not other human chromosomes. Localization of the CSF3R on chromosome 1 was further confirmed by the spot-blot hybridization of sorted human chromosomes.
Inazawa
J
J
Department of Hygiene, Kyoto Prefectural University of Medicine, Japan.
Fukunaga
R
R
Seto
Y
Y
Nakagawa
H
H
Misawa
S
S
Abe
T
T
Nagata
S
S
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000235:genetics*
D015345:Oligonucleotide Probes; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D004247:DNA