The human interferon-gamma gene contains an inducible promoter that can be transactivated by tax I and II.
T cells infected with the HTLV (human T cell leukemia virus)-I and -II retroviruses often express the lymphokine interferon-gamma (IFN-gamma). We sought the molecular explanation for this phenomena by co-transfecting the transactivator genes of these human retroviruses together with 5' flanking sequences of the human IFN-gamma gene linked to a heterologous reporter gene. In the presence of phytohemagglutinin or anti-T cell receptor monoclonal antibodies and phorbol 12-myristate 13-acetate, the IFN-gamma promoter is activated in human T cells, which can be further enhanced by co-transfection of the tax I or II genes. Using Bal-31 deletion analysis, we have found that induction by mitogens can be mapped to a region between -284 to -260 (bp) 5' of the transcriptional start site, which is separable from the sequence(s) further upstream which transmit the synergistic transactivation by tax I or II.
Brown
D A
DA
Division of Immunology, Beckman Research Institute of the City of Hope, Duarte, CA 91010.
Nelson
F B
FB
Reinherz
E L
EL
Diamond
D J
DJ
D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology; D001483:Base Sequence; D017252:CD3 Complex; D003001:Cloning, Molecular; D003524:Cyclosporins / Q000494:pharmacology; D016356:Gene Products, tax / Q000494:pharmacology*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic; D014162:Transfection
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D003524:Cyclosporins; D016356:Gene Products, tax; D011948:Receptors, Antigen, T-Cell; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate