title: Epidemiological survey of Trypanosoma cruzi infection in north-eastern Brazil using different diagnostic methods.
abstract: A survey of the prevalence of Trypanosoma cruzi infection was carried out in Oitis, a small community in the State of Piaui, Brazil. Two hundred and sixty five individuals were screened by microscopic examination, hemoculture, indirect immunofluorescence (IFA), enzyme-linked immunosorbent assay (ELISA), and competitive enzyme-linked immunosorbent assay (C-ELISA) using the monoclonal antibody TCF87 against to a 25kd T. cruzi antigen. Seropositivity was 14.3% by the IFA test, 14.7% by ELISA, and 13.2% by C-ELISA. The C-ELISA using the TCF87 monoclonal antibody seems to be applicable in serodiagnosis of Chagas' disease.
authors.affiliation: Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, PE, Brasil.
authors.forename: J
authors.initials: J
authors.lastname: Figuerêdo-Silva
authors.forename: Y
authors.initials: Y
authors.lastname: Kaneda
authors.forename: H
authors.initials: H
authors.lastname: Tachibana
authors.forename: R
authors.initials: R
authors.lastname: Furushima
authors.forename: S
authors.initials: S
authors.lastname: Tateno
authors.forename: F G
authors.initials: FG
authors.lastname: Correia-Lima
authors.forename: D N
authors.initials: DN
authors.lastname: Bento
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D001938:Brazil / Q000453:epidemiology; D014355:Chagas Disease / Q000175:diagnosis / Q000453:epidemiology*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D015995:Prevalence