title: Effects of sodium pentobarbital anesthesia on blood flow in skin, myocutaneous, and fasciocutaneous flaps in swine.
abstract: Drug effect on flap blood flow is most commonly determined in anesthetized animals, yet the effect of the anesthetic is often poorly understood. Halothane and nitrous oxide cause profound changes in skin blood flow and thus provide an unsuitable anesthetic technique for use in measuring drug effects on skin and myocutaneous flaps in swine. The goal of this study was to determine the effects of sodium pentobarbital anesthesia on cardiovascular parameters and blood flow in skin, myocutaneous, and fasciocutaneous flaps in pigs. In seven pigs, 7 forelimb skin flaps, 7 forelimb fasciocutaneous flaps, 14 arterial buttock flaps, and 14 latissimus dorsi flaps were created. Blood flow was measured at 2-cm intervals along each flap while the animal was awake and anesthetized. A cardiac depressant effect of pentobarbital was observed, but no change in blood flow could be demonstrated in control skin or control muscle. However, pentobarbital did significantly increase blood flow in all viable portions of arterial and random skin flaps, fasciocutaneous flaps, and the cutaneous segments of the latissimus dorsi flap. These demonstrated effects of pentobarbital should be taken into consideration in designing and analyzing studies of flap blood flow in the acute postoperative phase.
authors.affiliation: Microsurgical Laboratories, Royal Victoria Hospital, Montreal, Quebec, Canada.
authors.forename: J G
authors.initials: JG
authors.lastname: Thomson
authors.forename: C L
authors.initials: CL
authors.lastname: Kerrigan
authors.forename: M
authors.initials: M
authors.lastname: Abrahamowicz
mesh_terms: D000704:Analysis of Variance; D000771:Anesthesia, Intravenous / Q000009:adverse effects*; D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects; D005205:Fascia / Q000098:blood supply; D009132:Muscles / Q000098:blood supply; D010424:Pentobarbital / Q000009:adverse effects*; D012039:Regional Blood Flow / Q000187:drug effects*; D012867:Skin / Q000098:blood supply; D013524:Surgical Flaps / Q000502:physiology*; D013552:Swine; D013997:Time Factors
chemical_list: D010424:Pentobarbital