Diabetes-induced decrease in the mRNA coding for sarcoplasmic reticulum Ca(2+)-ATPase in adult rat cardiomyocytes.
The present study examined the level of the mRNA coding for the sarcoplasmic reticulum (SR) Ca(2+)-ATPase in isolated ventricular myocytes from streptozotocin-diabetic rats and genetically obese (fa/fa) rats using Northern blotting techniques. In both animal models one single transcript with a mean size of 4.12 kb could be detected. In insulin-deficient rats the Ca(2+)-ATPase transcript level decreased by 51% when compared to normal rats. In obese animals a modest decrease in the Ca(2+)-ATPase mRNA content to 77% of lean controls has been detected. Decreased mRNA expression of the SR Ca(2+)-ATPase may partly explain the delayed diastolic relaxation observed in the diabetic heart.
Russ
M
M
Laboratory of Molecular Cardiology, Diabetes Research Institute, Düsseldorf, Federal Republic of Germany.
Reinauer
H
H
Eckel
J
J
D000818:Animals; D015152:Blotting, Northern; D000252:Calcium-Transporting ATPases / Q000235:genetics*; D002478:Cells, Cultured; D015342:DNA Probes; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D014158:Transcription, Genetic
D015342:DNA Probes; D012333:RNA, Messenger; D000252:Calcium-Transporting ATPases