Is Cyclin Zeuthen's "division protein"?
Biochemical evidence is presented for the presence of cyclin in Tetrahymena. Zeuthen previously postulated the existence of a heat-labile "division protein" to explain heat-shock-induced division synchrony in Tetrahymena [(1964) Synchrony in Cell Division and Growth (Zeuthen, E., Ed.), pp. 99-158, Interscience, New York]. We show that cyclin is heat-labile in Tetrahymena and suggest that cyclin may be Zeuthen's division protein. Cyclin and cell cycle control is of interest in Tetrahymena because the division mechanism drives macronuclear amitosis, closed and acentric micronuclear mitosis, and cortical differentiation in this cell type.
Williams
N E
NE
Department of Biology, University of Iowa, Iowa City 52242.
Macey
M G
MG
D000818:Animals; D001345:Autoradiography; D002453:Cell Cycle; D002455:Cell Division; D016213:Cyclins / Q000502:physiology*; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification; D013462:Sulfur Radioisotopes; D013769:Tetrahymena pyriformis / Q000166:cytology / Q000254:growth & development*
D016213:Cyclins; D011506:Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine