title: Inhibition of gastric mucosal laminin receptor by Helicobacter pylori lipopolysaccharide.
abstract: Laminin receptor was isolated from gastric epithelial cell membrane by the procedure involving membrane solubilization with octylglucoside followed by affinity chromatography on laminin-coupled Sepharose. The receptor protein, eluted from the matrix with cation-free EDTA buffer, yielded on SDS-PAGE a single 67kDa band. After radioiodination, the protein was incorporated into liposomes which displayed specific affinity toward the laminin-coated surface. The binding of liposomal receptor to the laminin-coated surface was inhibited by lipopolysaccharide from H.pylori. The inhibitory effect was proportional to the concentration of lipopolysaccharide up to 50 micrograms/ml at which point a 96% decrease in the receptor binding occurred. It is suggested that a similar process may account for the loss of mucosal integrity in the pathogenesis of H. pylori associated gastric disease.
authors.affiliation: Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
authors.forename: B L
authors.initials: BL
authors.lastname: Slomiany
authors.forename: J
authors.initials: J
authors.lastname: Piotrowski
authors.forename: S
authors.initials: S
authors.lastname: Sengupta
authors.forename: A
authors.initials: A
authors.lastname: Slomiany
mesh_terms: D000818:Animals; D001419:Bacteria / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D002846:Chromatography, Affinity; D004848:Epithelium / Q000276:immunology; D005753:Gastric Mucosa / Q000276:immunology*; D005960:Glucosides; D016480:Helicobacter pylori / Q000276:immunology*; D007700:Kinetics; D007797:Laminin / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008081:Liposomes; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011971:Receptors, Immunologic / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D017474:Receptors, Laminin
chemical_list: D005960:Glucosides; D007797:Laminin; D008070:Lipopolysaccharides; D008081:Liposomes; D011971:Receptors, Immunologic; D017474:Receptors, Laminin; C018619:octyl-beta-D-glucoside