Expression of vasopressin V2 receptor in Xenopus laevis oocytes by porcine kidney cell line (LLC-PK1) messenger RNA.
Vasopressin V2 receptor was expressed in Xenopus laevis oocytes which were injected with poly(A) +RNA from porcine kidney cell line LLC-PK1. Pharmacological antagonism of the expressed V2 receptor was observed between arginine vasopressin and two potent and selective vasopressin antagonists: [d(CH2)5, D2-Phe2 Ile4, Ala9-NH2]arginine vasopressin and [d(CH2)5,D-Ile2, Ile4]arginine vasopressin. Activation constant for arginine vasopressin concentration was 1.32 x 10(-10)M. The nucleotide length of the mRNA encoding for vasopressin V2 receptor was deduced to be approximately 2 kilobases.
Kimura
K
K
Department of Pharmacology, Faculty of Medicine, University of Tokyo, Japan.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D007668:Kidney / Q000378:metabolism; D009865:Oocytes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D011945:Receptors, Angiotensin / Q000096:biosynthesis / Q000235:genetics*; D017483:Receptors, Vasopressin; D013552:Swine; D014170:Transformation, Genetic; D014667:Vasopressins / Q000494:pharmacology; D014982:Xenopus laevis / Q000235:genetics*
D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D017483:Receptors, Vasopressin; D014667:Vasopressins; D000262:Adenylyl Cyclases