title: Similarity of the three-dimensional structures of actin and the ATPase fragment of a 70-kDa heat shock cognate protein.
abstract: Although there is very little sequence identity between the two proteins, the structures of rabbit skeletal muscle actin (375-amino acid residues) and the 44-kDa ATPase fragment of the bovine 70-kDa heat shock cognate protein (HSC70; 386 residues) are very similar. The alpha-carbon positions of 241 pairs of amino acid residues that are structurally equivalent within the two proteins can be superimposed with a root-mean-square difference in distance of 2.3 A; of these, 39 residues are identical, and 56 are conservative substitutions. In addition, the conformations of ADP are very similar in both proteins. A local sequence "fingerprint," which may be diagnostic of the adenine nucleotide beta-phosphate-binding pocket, has been derived. The fingerprint identifies members of the glycerol kinase family as candidates likely to have a similar structure in their nucleotide-binding domains. The structural differences between the two molecules mainly occur in loop regions of actin known to be involved in interactions with other monomers in the actin filament or in the binding of myosin; the corresponding regions in heat shock proteins may have functions that are as yet undetermined. Placing the Ca2+ ATP of actin on the ATPase fragment structure suggests Asp-206 (corresponding to His-161 of actin) as a candidate proton acceptor for the ATPase reaction.
authors.affiliation: Beckman Laboratories for Structural Biology, Department of Cell Biology, Stanford University School of Medicine, CA 94305-5400.
authors.forename: K M
authors.initials: KM
authors.lastname: Flaherty
authors.forename: D B
authors.initials: DB
authors.lastname: McKay
authors.forename: W
authors.initials: W
authors.lastname: Kabsch
authors.forename: K C
authors.initials: KC
authors.lastname: Holmes
mesh_terms: D000199:Actins / Q000737:chemistry* / Q000235:genetics; D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011487:Protein Conformation; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid; D014961:X-Ray Diffraction
chemical_list: D000199:Actins; D006360:Heat-Shock Proteins; D000251:Adenosine Triphosphatases