Inhibition of methyl methanesulfonate-induced unscheduled DNA synthesis in spermatozoa of mice by Poly I-C.
Methyl methanesulfonate (MMS 75 mg.kg-1)-induced unscheduled DNA synthesis (UDS) was inhibited significantly in the spermatozoa of mice injected with Poly I-C (0.05, 0.50, and 5.00 mg.kg-1) at 4 h prior to ip MMS. Radioactivity was reduced from 53 +/- 3 to 50, 45 +/- 5, and 34 +/- 6 dpm/million sperms respectively. The effects of Poly I-C were dose-dependent (r = 0.9498, P less than 0.05) in inhibition of MMS-induced UDS. The effect of serum collected from the mice injected with Poly I-C (0.50 mg.kg-1) had similar effects as that of Poly I-C. Our findings suggest that both Poly I-C alone and mouse serum induced with Poly I-C may prevent the DNA damage produced by MMS.
Yang
B X
BX
Department of Medical Genetics, Norman Bethune University of Medical Science, Changchun, China.
Su
X Y
XY
Zhu
Y Z
YZ
Sun
K
K
Xu
D Q
DQ
Lu
Z Q
ZQ
Jin
Y H
YH
D000818:Animals; D004247:DNA / Q000096:biosynthesis* / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D008297:Male; D008741:Methyl Methanesulfonate / Q000037:antagonists & inhibitors*; D051379:Mice; D008810:Mice, Inbred C57BL; D011070:Poly I-C / Q000494:pharmacology*; D013094:Spermatozoa / Q000187:drug effects
D004247:DNA; D008741:Methyl Methanesulfonate; D011070:Poly I-C