title: Molecular cloning and characterization of genes expressed in shoot apical meristems.
abstract: The above-ground portion of a plant develops from the shoot apical meristem. An abundant source of apical meristems was obtained from cauliflower heads. Meristematic cDNAs were identified by differential screening and used to isolate corresponding Arabidopsis thaliana genes. Transcriptional promoters from Arabidopsis clones were fused to the beta-glucuronidase (GUS) reporter gene and introduced into plants, and GUS expression was used to analyze temporal and spatial regulation of the promoters. One promoter (meri-5) directed GUS expression in the meristematic dome and not the surrounding leaf primordia. The meri-5 promoter also directed GUS expression at branching points in the shoot and root. A second meristematic gene was found to be a histone (H3) gene. The H3 promoter was isolated and fused to GUS. Expression of the H3-GUS fusion in transgenic tobacco showed preferential expression in the peripheral zone and a lack of noticeable staining in the central zone.
authors.affiliation: Plant Molecular Biology Group, Monsanto Company, St. Louis, Missouri 63198.
authors.forename: J I
authors.initials: JI
authors.lastname: Medford
authors.forename: J S
authors.initials: JS
authors.lastname: Elmer
authors.forename: H J
authors.initials: HJ
authors.lastname: Klee
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins; D001483:Base Sequence; D001937:Brassica / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D004247:DNA; D005786:Gene Expression Regulation; D017343:Genes, Plant; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D016695:Glycosyltransferases; D008969:Molecular Sequence Data; D005810:Multigene Family; D009928:Organ Specificity / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic
chemical_list: D029681:Arabidopsis Proteins; D010940:Plant Proteins; D004247:DNA; D016695:Glycosyltransferases; C000627068:XTH24 protein, Arabidopsis; D005966:Glucuronidase