Distribution of Photofrin between tumour cells and tumour associated macrophages.
Photofrin levels in cells derived from SCCVII tumours, excised from mice that previously received the drug, were measured using a fluorescence activated cell sorter (FACS). Concomitantly, in the same cells the FACS was used to measure fluorescein isothiocyanate (FITC) fluorescence that originated from FITC-conjugated antimouse IgG added to the cell suspension before sorting. This later measurement enabled discrimination between IgG negative tumour malignant cells and IgG positive host cells (primarily macrophages). In addition, cellular Photofrin content in 'tumour' and 'host' cells sorted by FACS was determined by chemical extraction. The measurements were performed for the time intervals 1-96 h post Photofrin administration. The data showed consistently higher Photofrin levels in the 'host cells', i.e., tumour associated macrophages (TAM), than in 'tumour' cells. On a per cell basis, at any time point studied there was a minimum of 1.7 times more Photofrin in 'host' than in 'tumour cells', while at 4-12 h postadministration, ratios of up to 3.0 times were observed. This corresponds to ratio values greater than 9, when based on Photofrin content per micrograms cell protein.
Korbelik
M
M
Cancer Imaging Unit, British Columbia Cancer Research Centre, Vancouver, Canada.
Krosl
G
G
Olive
P L
PL
Chaplin
D J
DJ
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D002448:Cell Adhesion; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D016650:Fluorescein-5-isothiocyanate; D017324:Hematoporphyrin Derivative; D006415:Hematoporphyrins / Q000493:pharmacokinetics*; D007074:Immunoglobulin G / Q000032:analysis; D008264:Macrophages / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D011838:Radiation-Sensitizing Agents / Q000493:pharmacokinetics*; D013997:Time Factors
D006415:Hematoporphyrins; D007074:Immunoglobulin G; D011838:Radiation-Sensitizing Agents; D017324:Hematoporphyrin Derivative; D016650:Fluorescein-5-isothiocyanate