In situ hybridization reveals VIP precursor mRNA-containing neurons in monkey and rat neocortex.
The cDNAs encoding monkey vasoactive intestinal polypeptide (VIP) and PHM-27 and rat VIP and PHI-27 were cloned and used to generate antisense RNA probes. Using in situ hybridization, neurons expressing the VIP/PHM or VIP/PHI precursor mRNAs were localized in monkey and rat somatic sensory and visual cortex. In both neocortical areas of both species, labeled cells were observed in all 6 layers as well as the subcortical white matter.
Benson
D L
DL
Department of Anatomy and Neurobiology, University of California, Irvine 92717.
Isackson
P J
PJ
Jones
E G
EG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002540:Cerebral Cortex / Q000737:chemistry*; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D008251:Macaca / Q000378:metabolism*; D008969:Molecular Sequence Data; D009474:Neurons / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D010451:Peptide PHI / Q000096:biosynthesis* / Q000235:genetics; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014660:Vasoactive Intestinal Peptide / Q000096:biosynthesis* / Q000235:genetics
D010451:Peptide PHI; D015347:RNA Probes; D012333:RNA, Messenger; D014660:Vasoactive Intestinal Peptide; D004247:DNA