Detection of serum antibodies using LBC cell-grown sialodacryoadenitis (SDA) virus.
The TG strain of sialodacryoadenitis (SDA) virus propagated in LBC cell culture (TGr/LBC) reacted strongly with anti-TGr rat serum in complement fixation (CF) tests, showing much higher titers with anti-TGr rat serum than mouse hepatitis virus MHV-NuU antigen. The antigenicity was not affected after ether treatment while infectivity was lost. The TGr/LBC antigen might be useful in seromonitoring for SDA infection in rats.
Utsumi
K
K
Research Laboratories, Dainippon Pharmaceutical Co., Ltd., Osaka, Japan.
Ishikawa
T
T
Yokota
Y
Y
Yoshida
K
K
Maeda
K
K
Ohnishi
K
K
Yanabe
M
M
Fujiwara
K
K
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002478:Cells, Cultured; D003168:Complement Fixation Tests / Q000662:veterinary; D003332:Coronaviridae / Q000276:immunology*; D003333:Coronaviridae Infections / Q000276:immunology; D007962:Leukocytes / Q000166:cytology* / Q000382:microbiology; D051379:Mice; D008813:Mice, Inbred ICR; D051381:Rats; D011918:Rats, Inbred SHR
D000914:Antibodies, Viral