Molecular cloning and characterization of a rat brain cDNA encoding a 5-hydroxytryptamine1B receptor.
To date, there have been at least eight different receptors for the neurotransmitter serotonin (5-HT) identified in the central nervous system. These receptors fall into four pharmacological classes: 5-HT1, 5-HT2, 5-HT3 and 5-HT4. The 5-HT1 class has been shown to contain at least four pharmacologically distinct subtypes, 5-HT1A-D. Of these, cDNAs encoding the 5-HT1A and 5-HT1C receptors have been previously characterized. We now report the cloning and expression of a rat brain cDNA encoding another member of the 5-HT1 receptor family. Transient expression of this clone demonstrated high-affinity binding of [3H]5-HT with a pharmacological profile corresponding to that of the 5-HT1B subtype: 5-CT, 5-HT greater than propranolol greater than methysergide greater than rauwolscine greater than 8-OH-DPAT. In situ hybridization revealed expression of cognate mRNA within cells of the dorsal and median raphe nuclei, consistent with previous reports that the 5-HT1B receptor acts as an autoreceptor on 5-HT terminals in this species. mRNA expression was also detected in cells within the CA1 region of hippocampus, striatum, layer 4 of cortex and in the cerebellum, suggesting a previously unrecognized post-synaptic role for the 5-HT1B receptor.
Voigt
M M
MM
Laboratory of Molecular Neuroendocrinology, University of Heidelberg, FRG.
Laurie
D J
DJ
Seeburg
P H
PH
Bach
A
A
D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D006801:Humans; D008784:Methysergide / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011433:Propranolol / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D011869:Radioligand Assay; D051381:Rats; D011985:Receptors, Serotonin / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D012701:Serotonin / Q000031:analogs & derivatives / Q000378:metabolism; D013764:Tetrahydronaphthalenes / Q000378:metabolism; D014162:Transfection; D015016:Yohimbine / Q000378:metabolism
D012333:RNA, Messenger; D011985:Receptors, Serotonin; D013764:Tetrahydronaphthalenes; D015016:Yohimbine; D012701:Serotonin; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D004247:DNA; C047087:5-carboxamidotryptamine; D011433:Propranolol; D008784:Methysergide