Characterization of phiLC3, a Lactococcus lactis subsp. cremoris temperature bacteriophage with cohesive single-stranded DNA ends.
The temperate bacteriophage phiLC3, isolated from Lactococcus lactis subsp. cremoris, has an isometric head and a flexible tail containing 1 major protein and 8 minor proteins. Infection of a permissive L. lactis host strain yields a burst of about 50 phages per infected cell with a latent period of 60 min. A detailed restriction map of the phage chromosome was constructed by using 12 different restriction enzymes. The phage chromosome is a 33-kb linear double-stranded DNA molecule with unique cohesive ends and with a G + C content of 36.5%. Chemical sequencing of the DNA ends revealed 13-base 3' extended complementary single strands with a relatively high percentage of G + C. Pulsed-field gel electrophoretic analysis of DNA from a strain lysogenized with phiLC3 was used to localize the prophage to a 320-kb BamHI restriction endonuclease fragment from the host chromosomal DNA. This result indicates that lysogeny involves integration of the phage into the host chromosome. A spontaneous phiLC3 clear plaque mutant that was unable to give rise to lysogens was isolated.
Lillehaug
D
D
Laboratory of Microbial Gene Technology, Oslo, Norway.
Lindqvist
B
B
Birkeland
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NK
D001435:Bacteriophages / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D001482:Base Composition; D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry*; D016679:Genome, Viral; D013294:Lactococcus lactis / Q000235:genetics*; D008242:Lysogeny; D008969:Molecular Sequence Data; D009154:Mutation; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D014764:Viral Proteins