title: Expression of NGF receptor and NGF receptor mRNA in the developing and adult rat retina.
abstract: Nerve growth factor (NGF) has been recently found to rescue axotomized retinal ganglion cells (RGCs) of the adult rat from degeneration. Because the trophic effect of NGF involves a receptor-coupling event, the characterization and cellular localization of the NGF receptor (NGFR) in the retina are essential to understanding the possible specific action of NGF in this district of the central nervous system. We report here that the NGFR mRNA is expressed in fetal, neonatal, and adult rat retina. Using monoclonal antibody 192-IgG to immunoprecipitate and immunohistochemically identify NGFR, we also found that the NGFR from the retina has a molecular weight identical to that of the NGFR from PC12 cells. The NGFR is localized on RGCs and Müller cells. Finally, following ligation of the optic nerve, NGFR-immunopositive material was found to accumulate both distal and proximal to the site of ligation, suggesting that RGC axons anterogradely and retrogradely transport the NGFR. These data raise the possibility that NGF may play a specific role in rat RGCs.
authors.affiliation: Fidia Research Laboratories, Abano Terme, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Carmignoto
authors.forename: M C
authors.initials: MC
authors.lastname: Comelli
authors.forename: P
authors.initials: P
authors.lastname: Candeo
authors.forename: L
authors.initials: L
authors.lastname: Cavicchioli
authors.forename: Q
authors.initials: Q
authors.lastname: Yan
authors.forename: A
authors.initials: A
authors.lastname: Merighi
authors.forename: L
authors.initials: L
authors.lastname: Maffei
mesh_terms: D000310:Adrenal Gland Neoplasms; D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D015342:DNA Probes; D004622:Embryo, Mammalian; D007150:Immunohistochemistry; D008297:Male; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000378:metabolism; D010673:Pheochromocytoma; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D017475:Receptors, Nerve Growth Factor; D012160:Retina / Q000166:cytology / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D015342:DNA Probes; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor