Cell wall antigenic homology in certain species of Saccharomyces and an enzyme-linked immunosorbent assay for rapid identification of S. cerevisiae.
Cell-wall antigenic studies on Saccharomyces species grown by fermentation in 20% glucose broth indicated S. cerevisiae to develop two antigens specific for this species. These antigens were heat-stable polysaccharides and agglutination studies using specific antiserum prepared by cross absorption indicated their localization on the cell-wall surfaces. Using species-specific antibody, the results presented describe a specific and rapid identification of S. cerevisiae by ELISA according to the classification system of Yarrow (1984).
Umesh-Kumar
S
S
Discipline of Microbiology and Sanitation, Central Food Technological Research Institute, Mysore, India.
Nagarajan
L
L
D000946:Antigens, Fungal / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D002473:Cell Wall / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D012440:Saccharomyces / Q000276:immunology*; D012441:Saccharomyces cerevisiae / Q000276:immunology / Q000302:isolation & purification*
D000946:Antigens, Fungal; D000954:Antigens, Surface