title: Production of [2-11C]thymidine for quantification of cellular proliferation with PET.
abstract: A three-step synthesis of [2-11C]thymidine, using [11C]urea as precursor, is described. [2-11C]Thymine was obtained by cyclization of [11C]urea and diethyl beta-methylmalate in fuming sulfuric acid. After purification of the reaction mixture, [2-11C]thymine and 2'-deoxyribose-1-phosphate were incubated in the presence of thymidine phosphorylase to form [2-11C]thymidine. The whole synthesis procedure, including purification and pharmaceutical package, was achieved within 60 min with a decay corrected yield of 30-35%.
authors.affiliation: Department of Gastroenterology, University of Louvain Medical School, Brussels, Belgium.
authors.forename: T
authors.initials: T
authors.lastname: Vander Borght
authors.forename: D
authors.initials: D
authors.lastname: Labar
authors.forename: S
authors.initials: S
authors.lastname: Pauwels
authors.forename: L
authors.initials: L
authors.lastname: Lambotte
mesh_terms: D002250:Carbon Radioisotopes; D002455:Cell Division; D013941:Thymine / Q000378:metabolism*; D014055:Tomography, Emission-Computed
chemical_list: D002250:Carbon Radioisotopes; D013941:Thymine