title: Mapping of ATP-dependent trypsin-sensitive sites on the beta chain of outer-arm dynein from sea urchin sperm flagella.
abstract: The beta chain of sea urchin outer-arm dynein showed a peculiar tryptic digestion pattern in the presence of ATP (or ADP) plus Vi. Examination of the molecular mass of the products formed by photocleavage of tryptic fragments indicated that the trypsin-sensitive sites on the 165-kDa ATP-binding polypeptide in the presence of ATP and Vi are located 15 kDa apart from its amino-terminus, 2 kDa apart from its carboxy-terminus, and near the middle portion between the adenine- and gamma-Pi-binding sites. On the other hand, the carboxy-terminal region of the beta chain, the 135-kDa polypeptide, was cleaved into a 96-kDa polypeptide by tryptic digestion in the presence of ATP and Vi. Peptide mapping of 135-kDa, 96-kDa, and carboxy-terminally truncated polypeptides of the 135-kDa polypeptide revealed that the 96-kDa region is located at the amino-terminal portion of the 135-kDa region. These results indicate that the changes of trypsin susceptibility of dynien beta chain caused by binding ADP and Vi occur not in local region but over an extensive region on the beta chain.
authors.affiliation: Department of Biology, College of Arts and Sciences, University of Tokyo.
authors.forename: K
authors.initials: K
authors.lastname: Inaba
authors.forename: K
authors.initials: K
authors.lastname: Ogawa
authors.forename: H
authors.initials: H
authors.lastname: Mohri
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001665:Binding Sites; D004398:Dyneins / Q000737:chemistry* / Q000276:immunology; D006868:Hydrolysis; D008297:Male; D008970:Molecular Weight; D010449:Peptide Mapping; D011487:Protein Conformation / Q000187:drug effects; D012617:Sea Urchins; D013082:Sperm Tail / Q000737:chemistry* / Q000187:drug effects; D014357:Trypsin / Q000494:pharmacology*; D014638:Vanadates / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D014638:Vanadates; D000255:Adenosine Triphosphate; D014357:Trypsin; D004398:Dyneins