[The effects of ilexonin A on AA metabolism].
Arachidonic acid (AA) and prostaglandin endoperoxide were used as substrates and pig lung microsomes provided enzymes to determine thromboxane B2 (TXB2) and 6-keto-PGF1 alpha levels in a study of the effects of ilexonin A and verapamil on the activities of cyclooxygenase, thromboxane A2 synthetase and prostacyclin synthetase in the AA metabolic pathway. The results indicated that both ilexonin A and verapamil inhibited the activities of all three enzymes. The inhibitory effect of verapamil on thromboxane A2 synthetase was stronger than that on prostacyclin synthetase. The activity of the latter was not inhibited by verapamil in concentrations lower than 100 mumol/L.
Wang
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L
Institute of Basic Medical Sciences, Beijing.
Wang
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Z
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis*; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D019746:Intramolecular Oxidoreductases; D007535:Isomerases / Q000378:metabolism; D008861:Microsomes; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D009930:Organic Chemicals; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011817:Rabbits; D013552:Swine; D013929:Thromboxane B2 / Q000096:biosynthesis*; D013930:Thromboxane-A Synthase / Q000378:metabolism; D014700:Verapamil / Q000494:pharmacology
D001095:Arachidonic Acids; D005343:Fibrinolytic Agents; D009930:Organic Chemicals; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D003577:Cytochrome P-450 Enzyme System; C043587:ilexonin A; D014700:Verapamil; D011451:Prostaglandin-Endoperoxide Synthases; D007535:Isomerases; D019746:Intramolecular Oxidoreductases; C022461:prostacyclin synthetase; D013930:Thromboxane-A Synthase