Discrimination between mammalian RNases H-1 and H-2.
The two principal RNases H in mammalian cells, H-1 and H-2, differ in their responses to sale, divalent metal, and sulfhydryl inhibition. Specific reaction conditions that provide unambiguous discrimination between RNases H-1 and H-2 with only two assays are described. The assays were used for identification in a new purification procedure for RNases H-1 and H-2.
Goulian
M
M
Department of Medicine, University of California, San Diego, La Jolla 92093-0613.
Heard
C J
CJ
D000818:Animals; D004722:Endoribonucleases / Q000302:isolation & purification*; D008722:Methods; D051379:Mice; D016914:Ribonuclease H; D014407:Tumor Cells, Cultured
D004722:Endoribonucleases; D016914:Ribonuclease H