A fibronectin receptor on Candida albicans mediates adherence of the fungus to extracellular matrix.
Binding of fibronectin, an extracellular matrix (ECM) protein, to Candida albicans was measured, and adherence of the fungus to immobilized ECM proteins, fibronectin, laminin, types I and IV collagen, and subendothelial ECM was studied. 125I-labeled fibronectin was inhibited from binding to the fungus by unlabeled human plasma fibronectin and by Arg-Gly-Asp (RGD), Gly-Arg-Gly-Glu-Ser-Pro (GRGESP), and Gly-Arg-Gly-Asp-Thr-Pro (GRGDTP), but binding was not inhibited by Gly-Arg-Gly-Asp-Ser-Pro. Soluble fibronectin, RGD, GRGESP, and GRGDTP also inhibited fungal adherence to the individual immobilized ECM proteins in a complex pattern, but only soluble fibronectin (10(-7) M) inhibited fungal adherence to subendothelial ECM. Thus, C. albicans possesses at least one type of cell surface receptor for binding soluble fibronectin that can be inhibited with peptides. This receptor apparently is used to bind the fungus to immobilized ECM proteins and to subendothelial ECM and may play a role in the initiation of disseminated disease by bloodborne fungi by providing for adherence of the microorganisms to ECM proteins.
Klotz
S A
SA
Department of Medicine, Overton Brooks VA Medical Center, Shreveport.
Smith
R L
RL
D000595:Amino Acid Sequence; D002176:Candida albicans / Q000378:metabolism* / Q000502:physiology; D005109:Extracellular Matrix / Q000382:microbiology*; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008969:Molecular Sequence Data; D011485:Protein Binding; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000378:metabolism*
D005353:Fibronectins; D007457:Iodine Radioisotopes; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic