Inhibition of sarcoplasmic reticulum Ca(2+)-ATPase activity by cadmium, lead and mercury.
The effect of Cd2+, Pb2+ and Hg2+ on the Ca(2+)-ATPase activity of sarcoplasmic reticulum from rabbit muscle was studied. The concentration of relevant free and complex species for the assay conditions have been computed. As a result, ATP hydrolysis was found to be inhibited with an IC50 value of 950 nmol/l free Cd2+ or 95 nmol/l free Pb2+. Although calculation of the free Hg2+ was not possible, the comparison of the IC50 values for total metal ions show that Hg2+ is the strongest inhibitor of enzyme activity. The inhibition by Cd2+ seems to be independent of substrate concentration, whereas the inhibitory effect of Pb2+ is lowered in the presence of higher MgATP concentrations. Our data illustrate that the three heavy metals are potent inhibitors of the Ca2+ pump. Therefore low concentrations of these metal ions may disturb intracellular Ca2+ homeostasis and act on Ca(2+)-mediated cell functions.
Hechtenberg
S
S
Institut für Zellbiologie, Biochemie und Biotechnologie, Universität Bremen, BRD.
Beyersmann
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D
D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D000252:Calcium-Transporting ATPases / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D007854:Lead / Q000494:pharmacology*; D008628:Mercury / Q000494:pharmacology*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000201:enzymology*
D002104:Cadmium; D007854:Lead; D000252:Calcium-Transporting ATPases; D008628:Mercury