title: Detection of interleukin and interleukin-receptor mRNA in human heart by polymerase chain reaction.
abstract: Diminished cardiac contractility associated with inflammatory infiltration may be mediated by the release of interleukins. To test this hypothesis, we assessed the presence of interleukin and interleukin-receptor mRNAs in non-failing human heart and in endomyocardial biopsies from patients with dilated cardiomyopathy or inflammatory myocarditis. Only those interleukins expressed by non-circulating cells (interleukin-1 beta, -4, and -8) were detected in samples of human heart while interleukins specific for activated leukocytes (interleukins-1 alpha and -2) were not detected in any samples. While interleukin-1-receptor mRNA was present in samples from non-failing hearts and those with idiopathic myopathy, it was absent from patients with inflammatory myocarditis, suggesting receptor mRNA down-regulation.
authors.affiliation: Peter Belfer Laboratory for Molecular Biology of Heart Failure, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
authors.forename: R O
authors.initials: RO
authors.lastname: Han
authors.forename: P E
authors.initials: PE
authors.lastname: Ray
authors.forename: K L
authors.initials: KL
authors.lastname: Baughman
authors.forename: A M
authors.initials: AM
authors.lastname: Feldman
mesh_terms: D001483:Base Sequence; D009202:Cardiomyopathies / Q000378:metabolism*; D002311:Cardiomyopathy, Dilated / Q000378:metabolism; D007375:Interleukin-1 / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D016209:Interleukin-8 / Q000235:genetics; D007378:Interleukins / Q000235:genetics*; D008969:Molecular Sequence Data; D009205:Myocarditis / Q000378:metabolism; D009206:Myocardium / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D011971:Receptors, Immunologic / Q000235:genetics*; D017472:Receptors, Interleukin-1
chemical_list: D007375:Interleukin-1; D007376:Interleukin-2; D016209:Interleukin-8; D007378:Interleukins; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D015847:Interleukin-4