[Measurement of intracellular free Ca2+ concentration in dissociated rat brain cells using Fura-2/AM].
The intracellular free Ca2+ concentration was measured in freshly dissociated brain cells prepared from neonatal rats using the fluorescent Ca2+ indicator Fura-2/AM. Cytosolic Ca2+ concentration of resting cells was calculated to be 240 +/- 5 nmol/L. Depolarization with high K+ resulted in an over 100% increase in intracellular Ca2+ concentration, and this increase could be prevented or reversed by verapamil or nifedipine known to block voltage-sensitive Ca channels. These results suggest that the adoption of Fura-2/AM method in freshly dissociated rat brain cells is a useful and relatively easily applicable technique for monitoring intracellular Ca2+ changes.
Li
M
M
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Wang
J F
JF
Han
J S
JS
Zhang
J T
JT
D000818:Animals; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D001923:Brain Chemistry; D002118:Calcium / Q000032:analysis*; D005456:Fluorescent Dyes; D016257:Fura-2 / Q000031:analogs & derivatives*; D009543:Nifedipine / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D014700:Verapamil / Q000494:pharmacology
D005456:Fluorescent Dyes; C049925:fura-2-am; D014700:Verapamil; D009543:Nifedipine; D011188:Potassium; D002118:Calcium; D016257:Fura-2