title: Activation-associated marker proteins: peptide mapping and their expression in macrophage cell lines.
abstract: The activation-associated markers, p47b and p71/73, have been recognized as minor proteins in peritoneal and bone marrow culture-derived macrophages activated for tumor cell killing. Proteins with identical characteristics of migration on 2-dimensional gels and comparable Cleveland peptide maps are described here in macrophage cell lines that could be activated for tumor cell killing (J774A.1, RAW 264, UNC-2). Macrophage cell lines that could not be activated (P388D1 and PU5-1.8) did not express the markers. The expression of these markers in activatable macrophage cell lines strengthens their association with the activation process and provides a bulk source of the proteins for purification studies.
authors.affiliation: Wilkinson Laboratory for Cancer Research, University of Kansas Cancer Center, Kansas City.
authors.forename: D M
authors.initials: DM
authors.lastname: Pinson
authors.forename: T A
authors.initials: TA
authors.lastname: Phillips
authors.forename: J L
authors.initials: JL
authors.lastname: Pace
authors.forename: R J
authors.initials: RJ
authors.lastname: MacKay
authors.forename: S W
authors.initials: SW
authors.lastname: Russell
mesh_terms: D000818:Animals; D002460:Cell Line; D002373:Cyclic AMP Receptor Protein / Q000235:genetics*; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D010449:Peptide Mapping; D011506:Proteins / Q000276:immunology*
chemical_list: D002373:Cyclic AMP Receptor Protein; D011506:Proteins