title: A gene encoding an antigen recognized by cytolytic T lymphocytes on a human melanoma.
abstract: Many human melanoma tumors express antigens that are recognized in vitro by cytolytic T lymphocytes (CTLs) derived from the tumor-bearing patient. A gene was identified that directed the expression of antigen MZ2-E on a human melanoma cell line. This gene shows no similarity to known sequences and belongs to a family of at least three genes. It is expressed by the original melanoma cells, other melanoma cell lines, and by some tumor cells of other histological types. No expression was observed in a panel of normal tissues. Antigen MZ2-E appears to be presented by HLA-A1; anti-MZ2-E CTLs of the original patient recognized two melanoma cell lines of other HLA-A1 patients that expressed the gene. Thus, precisely targeted immunotherapy directed against antigen MZ2-E could be provided to individuals identified by HLA typing and analysis of the RNA of a small tumor sample.
authors.affiliation: Ludwig Institute for Cancer Research, Brussels, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: van der Bruggen
authors.forename: C
authors.initials: C
authors.lastname: Traversari
authors.forename: P
authors.initials: P
authors.lastname: Chomez
authors.forename: C
authors.initials: C
authors.lastname: Lurquin
authors.forename: E
authors.initials: E
authors.lastname: De Plaen
authors.forename: B
authors.initials: B
authors.lastname: Van den Eynde
authors.forename: A
authors.initials: A
authors.lastname: Knuth
authors.forename: T
authors.initials: T
authors.lastname: Boon
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D052138:Genes, Neoplasm; D006801:Humans; D008545:Melanoma / Q000276:immunology*; D058950:Melanoma-Specific Antigens; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; C072011:MAGEA1 protein, human; C072013:MAGEA3 protein, human; C072012:MAGEB2 protein, human; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D004247:DNA