title: Massive cluster impact mass spectrometry: a new desorption method for the analysis of large biomolecules.
abstract: A new ion desorption method is described that utilizes a primary beam of massive, multiply charged cluster ions to generate secondary ions of peptides in a glycerol matrix. The massive cluster ion beam is generated via electrohydrodynamic emission using a 1.5 M solution of ammonium acetate in 30% aqueous glycerol. Negative ion spectra of peptides obtained using this technique show greatly decreased relative intensities for fragment ions and 'chemical noise' background when compared to spectra obtained using a xenon atom primary beam. The near absence of fragments derived from radiation damage to the sample solution is attributed to the impact of primary particles with energies less than 1 eV/nucleon.
authors.affiliation: Phrasor Scientific Inc., Duarte, CA 91010.
authors.forename: J F
authors.initials: JF
authors.lastname: Mahoney
authors.forename: J
authors.initials: J
authors.lastname: Perel
authors.forename: S A
authors.initials: SA
authors.lastname: Ruatta
authors.forename: P A
authors.initials: PA
authors.lastname: Martino
authors.forename: S
authors.initials: S
authors.lastname: Husain
authors.forename: T D
authors.initials: TD
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D004563:Electrochemistry; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*
chemical_list: D010455:Peptides