[Effective reconstitution of sarcoplasmic reticulum Ca2+-ATPase using lubrol PX].
Ca2(+)-ATPase of sarcoplasmic reticulum was reconstituted in the proteoliposomes by the salting out procedure. Triton X-100, C12E8 and Lubrol PX were used for the solubilization of the Ca2(+)-ATPase. Using fluorescent probes (diS-C3-(5), chlortetracycline) as well pH-measuring method, the functional of the reconstituted Ca2(+)-ATPase was comparatively studied in three types of proteoliposomes. The efficiency of Ca2(+)-ATPase grew in the following detergent order: Triton X-100, C12E8, Lubrol PX.
Vinokurov
M G
MG
Pechatnikov
V A
VA
D000252:Calcium-Transporting ATPases / Q000737:chemistry*; D003902:Detergents; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000737:chemistry*; D017830:Octoxynol; D000077423:Polidocanol; D011092:Polyethylene Glycols; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D012995:Solubility
D003902:Detergents; D005456:Fluorescent Dyes; D008081:Liposomes; D000077423:Polidocanol; D011092:Polyethylene Glycols; D017830:Octoxynol; D000252:Calcium-Transporting ATPases
Effektivnaia rekonstruktsiia Ca2+-ATP-azy sarkoplazmaticheskogo retikuluma s ispol'zovaniem lubrola PX.