[Stimulation of calcium intake by growth-hormone-releasing-hormone in GH3 cells].
The effect of GH-RH in the intra-cytosolic free Ca2+ concentration was studied in GH3 cells. To this end, we have used microspectrofluorimetry performed on single cells. We show that 60% of cells respond to a brief application of 100 nM GH-RH by an increase of their [Ca2+]i (mean increase 100% over basal values). This response which is blocked by calcium channel inhibitors results from an increased influx of Ca2+ ions from the external medium.
Bresson
L
L
Laboratoire de Neurophysiologie, CNRS URA 1200, Université de Bordeaux II.
Fahmi
M
M
Sartor
P
P
Mariot
P
P
Dufy-Barbe
L
L
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002461:Cell Line, Transformed / Q000187:drug effects / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D013007:Growth Hormone-Releasing Hormone / Q000494:pharmacology*; D010911:Pituitary Neoplasms / Q000473:pathology*; D051381:Rats
D013007:Growth Hormone-Releasing Hormone; D002118:Calcium
Stimulation de l'entrée de calcium par le Growth-Hormone-Releasing-Hormone dans les cellules GH3.