Binding sites for atrial natriuretic peptide (ANP) on cultured pituicytes: lack of effect of ANP on release of neurohypophysial hormones in vitro.
Receptors for atrial natriuretic peptide (ANP) are known to be present in the posterior pituitary gland and this is a possible site of action of ANP to modulate neurohypophysial hormone release. Pituicytes cultured from adult rat neurohypophyses are shown to possess high affinity binding sites for ANP, suggesting that in vivo a population of neurohypophysial ANP receptors are present on these astrocytic glial cells. alpha-rANP (1-100 nM) did not modulate the basal or electrically stimulated release of oxytocin or vasopressin from the isolated neurohypophysis in vitro. The physiological significance of the glial ANP binding sites thus remains unknown.
Luckman
S M
SM
Department of Neuroendocrinology, AFRC Institute of Animal Physiology & Genetics Research, Babraham, Cambridge, U.K.
Bicknell
R J
RJ
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism* / Q000494:pharmacology; D001665:Binding Sites; D002478:Cells, Cultured; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008297:Male; D010121:Oxytocin / Q000378:metabolism; D010902:Pituitary Gland / Q000166:cytology; D010904:Pituitary Gland, Posterior / Q000378:metabolism*; D010907:Pituitary Hormones / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins; D014667:Vasopressins / Q000378:metabolism
D010907:Pituitary Hormones; D011994:Recombinant Proteins; D014667:Vasopressins; D010121:Oxytocin; D009320:Atrial Natriuretic Factor