Performance of 133 compounds in the lambda prophage induction endpoint of the Microscreen assay and a comparison with S. typhimurium mutagenicity and rodent carcinogenicity assays.
The Microscreen assay was developed as a means of testing very small samples, as in complex mixture fractionation. It is a multi-endpoint assay which utilizes E. coli WP2s(lambda). Exposure takes place to serial dilutions of the test compound in microtitre wells (250 microliters) followed by sampling from wells in which growth has occurred ('non-toxic wells'). Although a number of different endpoints can be measured, only the prophage induction endpoint (the first one developed) has been extensively tested. Results with 133 compounds are presented. These include 111 compounds which have been tested in the S. typhimurium assay and 66 compounds for which both rodent bioassay and S. typhimurium assay data exists. The concordance for the Microscreen assay and the S. typhimurium assay was 71%. For this group of compounds, the sensitivity of the Microscreen assay in detecting carcinogens was 76% compared with 58% for the S. typhimurium assay. However, the S. typhimurium assay was somewhat more specific (69%) compared with the Microscreen (56%). The overall association between carcinogenicity and Microscreen results was statistically significant (p = 0.029), whereas for the S. typhimurium assay the association with carcinogenicity was non-significant (p = 0.086). The Microscreen assay was able to detect halogenated compounds better than the S. typhimurium assay. The Microscreen assay should prove useful in complex mixture fractionation, or in other situations where sample size is limiting.
Rossman
T G
TG
Institute of Environmental Medicine, NYU Medical Center, Tuxedo 10987.
Molina
M
M
Meyer
L
L
Boone
P
P
Klein
C B
CB
Wang
Z
Z
Li
F
F
Lin
W C
WC
Kinney
P L
PL
D000818:Animals; D010582:Bacteriophage lambda / Q000187:drug effects*; D015197:Carcinogenicity Tests; D002273:Carcinogens; D004926:Escherichia coli; D009152:Mutagenicity Tests; D009153:Mutagens; D051381:Rats; D012377:Rodentia; D012486:Salmonella typhimurium / Q000187:drug effects*
D002273:Carcinogens; D009153:Mutagens