A method for steam-sterilizing semi-solid diets for germ-free mice research.
A semi-solid corn-meal diet sterilized in glass flasks in a hospital-type autoclave is described. The values of weight gain, feed efficiency, apparent digestibility, and cecal diameters were the same for germ-free mice fed either on this diet or on a soybean meal and alfalfa diet (L-485), widely used in germ-free research. The corn-meal diet has been successfully used for eight years.
Moraes-Santos
T
T
Instituto de Ciencias Biológicas, Universidade Federal de Minas Gerais, Brazil.
Nicoli
J R
JR
Silva
M E
ME
Vieira
E C
EC
D000704:Analysis of Variance; D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002364:Caseins; D003314:Corn Oil; D005433:Flour; D005526:Food, Formulated; D005856:Germ-Free Life; D000455:Medicago sativa; D051379:Mice; D012107:Research Design; D013227:Steam; D013242:Sterilization / Q000379:methods*; D015430:Weight Gain; D003313:Zea mays
D002364:Caseins; D013227:Steam; D003314:Corn Oil