title: Detection of arginine esterase activity in human follicular fluid.
abstract: One major fraction hydrolysing arginine ester and amido derivative (major preparation) was detected and separated from human follicular fluid by Cellulofine GCL-2000 gel filtration. The best ester and amide substrates for this preparation were acetyl-glycyl-lysine methyl ester (Ac-Gly-Lys-Me) and D-valyl-L-leucyl-L-arginine-p-nitroanilide (Val-Leu-Arg-pNA), respectively. The preparation contained plasmin and plasminogen; lysine-Cellulofine adsorption and elution changed the substrate specificity of its esterolytic activity. After treatment by lysine-Cellulofine adsorption and elution, the basic and acidic arginine esterase activities of the major preparation were separated by CM- and DEAE-cellulose adsorption and elution, respectively. The substrate specificity of these two esterolytic enzyme activities differed before and after adsorption of the major preparation to the lysine affinity column.
authors.affiliation: Department of Obstetrics and Gynaecology, School of Medicine, Keio University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: J Y
authors.initials: JY
authors.lastname: Park
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuda
authors.forename: S
authors.initials: S
authors.lastname: Kaneko
authors.forename: S
authors.initials: S
authors.lastname: Oshio
authors.forename: S
authors.initials: S
authors.lastname: Nozawa
authors.forename: Y
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: S
authors.initials: S
authors.lastname: Akihama
mesh_terms: D000595:Amino Acid Sequence; D002265:Carboxylic Ester Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D002850:Chromatography, Gel; D005260:Female; D005341:Fibrinolysin / Q000378:metabolism; D015571:Follicular Fluid / Q000201:enzymology*; D006801:Humans; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D009842:Oligopeptides; C019724:arginine esterase; D002265:Carboxylic Ester Hydrolases; D005341:Fibrinolysin