title: Inositol 1,4,5-trisphosphate receptors: distinct neuronal and nonneuronal forms derived by alternative splicing differ in phosphorylation.
abstract: We have identified two distinct transcripts of inositol 1,4,5-trisphosphate receptor by using the PCR on first-strand cDNAs from various rat tissues. The longer form, corresponding to the previously cloned adult rat brain inositol 1,4,5-trisphosphate receptor, contains a 120-nucleotide insert between the two cAMP-dependent protein kinase phosphorylation consensus sequences. The shorter form (lacking the insert) predominates in fetal brain and peripheral tissues and appears to represent a nonneuronal receptor, whereas the longer form is found in adult brain and appears to be exclusively neuronal. The phosphorylation kinetics by cAMP-dependent protein kinase and the phosphopeptide maps differ for inositol 1,4,5-trisphosphate receptors purified from tissues predominantly expressing different forms of the transcript.
authors.affiliation: Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
authors.forename: S K
authors.initials: SK
authors.lastname: Danoff
authors.forename: C D
authors.initials: CD
authors.lastname: Ferris
authors.forename: C
authors.initials: C
authors.lastname: Donath
authors.forename: G A
authors.initials: GA
authors.lastname: Fischer
authors.forename: S
authors.initials: S
authors.lastname: Munemitsu
authors.forename: A
authors.initials: A
authors.lastname: Ullrich
authors.forename: S H
authors.initials: SH
authors.lastname: Snyder
authors.forename: C A
authors.initials: CA
authors.lastname: Ross
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015220:Calcium Channels; D002531:Cerebellum / Q000378:metabolism*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007295:Inositol Phosphates / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D015345:Oligonucleotide Probes; D009928:Organ Specificity; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D011499:Protein Processing, Post-Translational; D012326:RNA Splicing; D051381:Rats; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear; D014649:Vas Deferens / Q000378:metabolism
chemical_list: D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007295:Inositol Phosphates; D015345:Oligonucleotide Probes; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D004247:DNA