Human corneal storage in modified McCarey-Kaufman and K-Sol media: effect on endothelial Na+/K+ ATPase pump site density and permeability.
Endothelial permeability (Pac) to carboxyfluorescein and Na+/K+ ATPase pump site density were determined in human corneas following storage for 4 or 7 days at 4 degrees C in either modified McCarey-Kaufman (mMK) or K-Sol media. Following 4 days of storage, Pac values for mMK- and K-Sol-preserved corneas were not significantly different from those of their prestorage mates. After 7 days of storage, however, corneas stored in K-Sol media showed a significant increase in Pac compared to their prestorage mates, whereas the mMK-stored corneas showed no change in Pac. Na+/K+ ATPase pump site density determined using [3H]ouabain was similar to a control group in the K-Sol-stored tissue but higher in the mMK-stored tissue following 7 days of storage. These studies suggest that mMK medium maintains endothelial barrier function and Na+/K+ ATPase pump site density at least as well as K-Sol medium through 7 days of corneal storage.
McDermott
M L
ML
Department of Ophthalmology, Wayne State University, Detroit, Michigan.
Watsky
M A
MA
Geroski
D H
DH
Edelhauser
H F
HF
D000328:Adult; D000368:Aged; D002809:Chondroitin Sulfates; D003315:Cornea / Q000201:enzymology / Q000378:metabolism; D003470:Culture Media; D004728:Endothelium, Corneal / Q000201:enzymology / Q000378:metabolism; D005452:Fluoresceins / Q000378:metabolism; D006531:HEPES; D006801:Humans; D008875:Middle Aged; D009930:Organic Chemicals; D010539:Permeability; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods
D003470:Culture Media; D005452:Fluoresceins; C051174:K-Sol preserving medium; C015368:McCarey-Kaufman medium; D009930:Organic Chemicals; C024098:6-carboxyfluorescein; D002809:Chondroitin Sulfates; D000254:Sodium-Potassium-Exchanging ATPase; D006531:HEPES