Novel anticoagulant peptides from the functional site of human placental anticoagulant protein.
Histidine-containing peptides SHLRKV and DHTLIR, corresponding to placental anticoagulant protein-I (PAP-I) residues 204-209 and 266-271, respectively, are included in the functional site of PAP-I and exhibit anticoagulant activity, but the peptides in which alanine is substituted for histidine do not. However, the peptide KHALKG, corresponding to the region from Lys-97 to Gly-102, did not exhibit an anticoagulant activity, showing that it is not included in the functional site. These findings thus suggest that His-205 and His-267 are involved in the Ca(2+)- or the phospholipid-binding site of PAP-I but that His-98 is not.
Funakoshi
T
T
Department of Hygienic Chemistry, Faculty of Pharmaceutical Science Kumamoto University, Japan.
Abe
M
M
Sakata
M
M
Shoji
S
S
Kubota
Y
Y
Shimada
H
H
Kojima
S
S
D000595:Amino Acid Sequence; D017304:Annexin A5; D000925:Anticoagulants / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006639:Histidine / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000737:chemistry / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid
D017304:Annexin A5; D000925:Anticoagulants; D002135:Calcium-Binding Proteins; D010446:Peptide Fragments; D011257:Pregnancy Proteins; D006639:Histidine