title: Controlled production of cirrhosis in the rat liver.
abstract: In the present study we propose a standardization of the method for the induction of fibrosis of the liver in rats by weekly administration of CCl4 by the intragastric route. The present method is a variation of that described by Proctor and Chatamra. The initial CCl4 dose is 0.04 ml (X dose) but the subsequent doses are calibrated using a 5% variation in animal weight as a reference point. For a weight gain of 5% or more, the successive doses are progressively increased by 50 to 150% (1.5X, 2X, 2.5X). When there is no variation in weight or loss in less than 5%, the previous dose is maintained. For a weight loss of more than 5%, the CCl4 dose is decreased by 50%. Consistent fibrosis if obtained as early as during the 8th week. Collagen content increases progressively from the 8th to the 12th week, reaching on average a level eight-fold higher than that observed in the control.
authors.affiliation: Laboratory of Hepatology, Federal University of Goiás School of Medicine, Brazil.
authors.forename: H
authors.initials: H
authors.lastname: Rosa
authors.forename: E R
authors.initials: ER
authors.lastname: Parise
authors.forename: F R
authors.initials: FR
authors.lastname: Paranhos
authors.forename: A L
authors.initials: AL
authors.lastname: Braga
authors.forename: A S
authors.initials: AS
authors.lastname: Freitas
authors.forename: A N
authors.initials: AN
authors.lastname: Hidalgo
authors.forename: M B
authors.initials: MB
authors.lastname: Mor
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002251:Carbon Tetrachloride / Q000008:administration & dosage*; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002251:Carbon Tetrachloride