The respiratory response to heat shock in Neurospora crassa.
A sharp decrease in oxygen uptake occurred in Neurospora crassa cells that were transferred from 30 degrees C to 45 degrees C, and the respiration that resumed later at 45 degrees C was cyanide-insensitive. Energization of mitochondria, measured in vivo with fluorescence microscopy and a carbocyanine dye, also declined sharply in cells at 45 degrees C. Electron microscopy showed no changes in mitochondrial complexity; however, the cytoplasm of heat-shocked cells was deficient in glycogen granules.
Habel
D
D
Department of Plant Biology, University of Minnesota, Saint Paul 55108.
Plesofsky-Vig
N
N
Brambl
R
R
D003486:Cyanides / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism; D006358:Hot Temperature; D007700:Kinetics; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D009492:Neurospora crassa / Q000378:metabolism* / Q000648:ultrastructure; D010101:Oxygen Consumption; D013172:Spores, Fungal / Q000378:metabolism; D013696:Temperature
D003486:Cyanides; D006003:Glycogen