Detection of IgM against hepatitis A virus using tissue culture derived antigen.
Sera from healthy donors and four groups of subjects with acute viral hepatitis were tested for anti-HAV IgM by ELISA with hepatitis A virus antigen grown in tissue culture. The results were compared with those obtained by formalin inactivated HAV from 'HAVAB-M' test kit (Abbott Laboratories, USA). With both preparations of antigens, sera from healthy donors and patients suffering from acute hepatitis B or non-A, non-B did not show anti-HAV IgM antibodies whereas acute phase sera from hepatitis A patients showed strong reactions indicating the presence of anti-HAV IgM antibodies. The results indicate similarity in the specificity of both preparations of HAV against anti-HAV antibodies and encourage the use of tissue culture derived HAV for serological diagnosis of hepatitis A.
Chitambar
S D
SD
National Institute of Virology, Pune.
Arankalle
V A
VA
Banerjee
K
K
D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006506:Hepatitis A / Q000276:immunology*; D035921:Hepatitis A Antigens; D006508:Hepatitis Antibodies / Q000097:blood*; D006507:Hepatovirus / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*
D000956:Antigens, Viral; D035921:Hepatitis A Antigens; D006508:Hepatitis Antibodies; D007075:Immunoglobulin M