Gamma-interferon promotes the release of IgE-binding factors (soluble CD23) by human epidermal Langerhans cells.
In a previous study, we have demonstrated that human epidermal Langerhans cells (LC) are induced to express Fc epsilon R2/CD23 by stimulation with IL-4 and/or IFN-gamma. In this study, using LC-enriched and LC-depleted epidermal cell (EC) cultures, we have shown that stimulation with IL-4 and/or IFN-gamma not only led to Fc epsilon R2/CD23 expression on normal human LC but also to the release of significant amounts of IgE-BF (soluble CD23). Furthermore, stimulation with IL-4 was more effective in induction of Fc epsilon R2/CD23 on LC when compared to IFN-gamma, which, in contrast, strongly promoted the release of IgE-BF. These results indicate that Fc epsilon R2/CD23-positive LC represent a potential source of IgE-BF and that, as is observed in monocytes or U937 cells, IFN-gamma and IL-4 differently regulate the Fc epsilon R2/CD23 expression and release on LC.
Bieber
T
T
Department of Dermatology, University of Munich, Germany.
Delespesse
G
G
D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism*; D002478:Cells, Cultured; D000078404:Epidermal Cells; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology; D007801:Langerhans Cells / Q000378:metabolism*; D011961:Receptors, Fc / Q000378:metabolism*; D017455:Receptors, IgE; D011994:Recombinant Proteins / Q000494:pharmacology
D000944:Antigens, Differentiation, B-Lymphocyte; D011961:Receptors, Fc; D017455:Receptors, IgE; D011994:Recombinant Proteins; D015847:Interleukin-4; D007371:Interferon-gamma