The effects of diet on the maximal activities of glutaminase, citrate synthase, hexokinase, 6-phosphofructokinase and lactate dehydrogenase in the skin of haired and hairless mice of various ages.
1. The maximal activities of hexokinase (HK), 6-phosphofructokinase (PFK), lactate dehydrogenase (LDH), citrate synthase (CS) and glutaminase (GLU) which provide a quantitative indices of flux through several important pathways have been measured in the skin of haired Balb/c and hairless Balb/c (nu/nu) mice under normal and dietary stress. 2. The skin of old haired mice exhibited higher PFK and LDH activities with lower HK, CS and GLU activities. All activities of enzymes associated with energy metabolism in the skin of old hairless mice were higher than those in the skin of haired mice. 3. HK, LDH, CS and GLU activities were maintained at normal levels in the skin of haired mice when these mice were fed diets deficient in energy or protein components (HPLE, LPNE). These enzymes however were severely suppressed when mice were fed a diet deficient in both energy and protein components (LPLE). Recovery of activities of these enzymes to the control level was observed when mice were refed with the normal diet for a week.
Nguyen
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Department of Microbiology, University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands.
Keast
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D000375:Aging / Q000378:metabolism; D000818:Animals; D002950:Citrate (si)-Synthase / Q000378:metabolism*; D004032:Diet; D004044:Dietary Proteins / Q000008:administration & dosage / Q000494:pharmacology; D002149:Energy Intake; D004734:Energy Metabolism; D005972:Glutaminase / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D012867:Skin / Q000201:enzymology*
D004044:Dietary Proteins; D007770:L-Lactate Dehydrogenase; D002950:Citrate (si)-Synthase; D006593:Hexokinase; D010732:Phosphofructokinase-1; D005972:Glutaminase