[Chromosomal localization of human X chromosome alphoid satellite DNA by in situ hybridization and its preliminary application].
In situ hybridization of human X chromosome-specific alphoid satellite DNA to human lymphocyte metaphase and interphase nuclei was carried out. The results showed that the probe hybridized with high specificity to the centromeric region at p11----q11 of the X chromosome. The number of silver grain clusters in the interphase nuclei was correlated with that of X chromosomes in the cells. 44% of the clusters were located near the nuclear membrane, the usual location of X chromatin (Barr body). The hybridized chromosomes were also R-banded with BrdU treatment, and the significance of this work was briefly discussed.
Qiu
H
H
Institute of Basic Medical Sciences, Beijing.
D002503:Centromere; D002871:Chromosome Banding; D015342:DNA Probes; D004276:DNA, Satellite / Q000032:analysis*; D005260:Female; D006801:Humans; D007399:Interphase; D008297:Male; D009693:Nucleic Acid Hybridization; D014960:X Chromosome
D015342:DNA Probes; D004276:DNA, Satellite