title: IL-4-producing murine T helper cell line provides help for in vitro production of IgE.
abstract: Studies of the regulation of the IgE synthesis have been hampered by the difficulties in making lymphocytes produce IgE in vitro. Synthesis of IgE, IgG1 and IgM were investigated in a murine coculture system in which a conalbumin-specific T helper cell line of Th2 type was cultured together with nonadherent splenocytes from normal MHC-matched mice. Help provided by the antigen-stimulated T cell line induced significant IgE production (20 ng/ml), along with IgG1 (5 micrograms/ml) and IgM (250 micrograms/ml). Immunoglobulin synthesis in cultures was detectable at day 3-4 and culminated at day 7-8. IL-4 production was observed within the first 2 days of culture. The kinetics of the synthesis of the three isotypes were parallel. Stimulation of the Th2 cell line with Con A produced dose-response curves resembling those after antigen stimulation, whereas supernatant from these cells was unable to induce synthesis of IgE. The presence of interferon-gamma in the cultures, inhibited synthesis of IgE, IgM and IgG1, but this inhibition was not due to interference with the IL-4 production, which increased in the presence of high doses of interferon-gamma.
authors.affiliation: Laboratory of Medical Allergology, National University Hospital, Copenhagen, Denmark.
authors.forename: L K
authors.initials: LK
authors.lastname: Poulsen
authors.forename: K
authors.initials: K
authors.lastname: Katamura
authors.forename: K
authors.initials: K
authors.lastname: Ishizaka
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003207:Conalbumin / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000502:physiology; D015847:Interleukin-4 / Q000502:physiology*; D051379:Mice; D008806:Mice, Inbred AKR; D008809:Mice, Inbred C3H; D011863:Radioimmunoassay; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D003208:Concanavalin A; D003207:Conalbumin; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma