Characterization of the promoter of the human gene encoding the high-affinity IgG receptor: transcriptional induction by gamma-interferon is mediated through common DNA response elements.
Expression of the high-affinity receptor for IgG (Fc gamma RI) is restricted to cells of myeloid lineage and is induced by gamma-interferon (IFN-gamma) but not by IFN-alpha/beta. The organization of the human Fc gamma RI gene has been determined and the DNA elements governing its cell type-restricted transcription and IFN-gamma induction are reported here. A 39-nucleotide sequence (IFN-gamma response region, or GRR) is defined that is both necessary and sufficient for IFN-gamma inducibility. Sequence analysis of the GRR reveals the presence of promoter elements initially defined for the major histocompatibility complex class II genes: i.e., X, H, and gamma-IRE sequences. Comparison of a number of genes whose expression is induced selectively by IFN-gamma indicates that the presence of these elements is a general feature of IFN-gamma-responsive genes. Our studies suggest that the combination of X, H, and gamma-IRE elements is a common motif in the pathway of transcriptional induction by this lymphokine.
Pearse
R N
RN
DeWitt Wallace Research Laboratory, Division of Molecular Biology, Sloan-Kettering Institute, New York, NY 10021.
Feinman
R
R
Ravetch
J V
JV
D000595:Amino Acid Sequence; D000818:Animals; D000943:Antigens, Differentiation / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D003360:Cosmids; D005260:Female; D005796:Genes / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology*; D051379:Mice; D008805:Mice, Inbred A; D008969:Molecular Sequence Data; D010920:Placenta / Q000276:immunology; D010957:Plasmids; D011247:Pregnancy; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011961:Receptors, Fc / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D017452:Receptors, IgG; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection
D000943:Antigens, Differentiation; D007074:Immunoglobulin G; D011961:Receptors, Fc; D017452:Receptors, IgG; D007371:Interferon-gamma