Specific assay for unconjugated dehydroepiandrosterone in human plasma by capillary gas chromatography with electron-capture detection.
A specific and sensitive method for the determination of unconjugated dehydroepiandrosterone in plasma is described. After extraction and purification of the extracts on a Celite column, the iodomethyldimethylsilyl ether derivative of dehydroepiandrosterone was isolated on an aluminium oxide column and assayed by gas chromatography with electron-capture detection. The method is sensitive: sample volumes of 0.5-1 ml are sufficient for the determination of dehydroepiandrosterone in plasma of normal male and female subjects aged 1-80 years. The assay is highly specific and has the potential to be used as a reference method for the determination of unconjugated dehydroepiandrosterone in biological samples.
Chabraoui
L
L
Department of Hormonology, Centre Hospitalier Lyon-Sud, Pierre Benite, France.
Mathian
B
B
Patricot
M C
MC
Revol
A
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D002849:Chromatography, Gas; D002852:Chromatography, Ion Exchange; D003687:Dehydroepiandrosterone / Q000097:blood*; D004563:Electrochemistry; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D012015:Reference Standards
D003687:Dehydroepiandrosterone