[3H]MK-801 binding to forebrain of E1 (epileptic) and non-epileptic strains of mice.
The El mouse is an animal model with genetically determined epilepsy. To elucidate the mechanism of convulsive seizures in El mice, the effects of L-glutamate and glycine on the binding of (+)[3H]MK-801 were studied in well-washed membranes from forebrains of ddY, BALB/c and El (stimulated and non-stimulated) mice. There were no significant differences in affinity (Kd) or density (Bmax) among the 4 groups of mice under basal conditions. Incubation in the presence of L-glutamate and/or glycine led to an increase in apparent density, but not in affinity. No significant change was observed in either Kd, Bmax, or the percentage increase in (+)[3H]MK-801 binding amount the 4 groups in the presence of L-glutamate and/or glycine. These results suggest that the seizure susceptibility of El mice cannot be explained by changes in affinity or density of the NMDA receptor/channel complex.
Kawasaki
H
H
Department of Neuropsychiatry, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Itoh
M
M
Nakahara
T
T
Fukahori
M
M
Akazawa
K
K
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D016291:Dizocilpine Maleate / Q000378:metabolism*; D004827:Epilepsy / Q000378:metabolism*; D005971:Glutamates / Q000494:pharmacology; D005998:Glycine / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D011869:Radioligand Assay; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism
D005971:Glutamates; D016194:Receptors, N-Methyl-D-Aspartate; D016291:Dizocilpine Maleate; D005998:Glycine