Comparison of peroxidase-labeled DNA probes with radioactive RNA probes for detection of human papillomaviruses by in situ hybridization in paraffin sections.
A study comparing in situ hybridization using nonradioactive DNA probes directly conjugated with horseradish peroxidase (HRP), and 35S-labeled antisense RNA probes for human papillomavirus (HPV) types 6/11, 16, and 18 was performed on formalin-fixed, paraffin-embedded tissue from 34 lesions of the cervix and vulva. These lesions included exophytic condylomas and intraepithelial and invasive neoplasms. HPV 6/11 was detected in two of four condylomata acuminata by both in situ techniques. HPV 16 was detected in 13 of 30 cases of intraepithelial and invasive neoplasms by both methods. Discordance between the two methods occurred in two instances. The radiolabeled probe but not the HRP probe detected HPV 16 in one case of cervical intraepithelial neoplasia (CIN 3), whereas the converse occurred in one case of vulvar intraepithelial neoplasia (VIN 3). HPV 18 was not detected in any of the specimens by either method. This study demonstrates that nonradioactive HRP-labeled probes for the detection of specific HPV types are as sensitive as the more laborious and potentially hazardous radioactive probes.
Park
J S
JS
Johns Hopkins Medical Institutions, Baltimore, Maryland.
Kurman
R J
RJ
Kessis
T D
TD
Shah
K V
KV
D015344:DNA Probes, HPV; D005260:Female; D005833:Genital Neoplasms, Female / Q000382:microbiology; D006735:Horseradish Peroxidase; D006801:Humans; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D015347:RNA Probes; D013462:Sulfur Radioisotopes; D014412:Tumor Virus Infections / Q000175:diagnosis*
D015344:DNA Probes, HPV; D015347:RNA Probes; D013462:Sulfur Radioisotopes; D006735:Horseradish Peroxidase