Transformation and establishment of fragile X cell lines from amniocytes.
Fragile X [fra(X)] cell lines have been established from an expressing 46,XX amniocyte culture. The amniocyte cells were transformed by the addition of an origin defective pSV40 vector. Fra(X) expression was observed at a frequency of 3% in the parental amniocyte cell line, and the transformed clones expressed the fra(X) site at a frequency of 9-13%. These cell lines maintain the cytogenetic phenotype and can serve as positive controls for testing the efficacy of the inducing systems during prenatal diagnostic studies.
Jenkins
L S
LS
University of California, San Francisco.
Gruenert
D C
DC
Jenkins
E C
EC
Schonberg
S A
SA
D000653:Amniotic Fluid / Q000166:cytology; D002461:Cell Line, Transformed; D003673:Defective Viruses; D005260:Female; D005600:Fragile X Syndrome / Q000473:pathology*; D006801:Humans; D010641:Phenotype; D011296:Prenatal Diagnosis; D012015:Reference Standards; D013539:Simian virus 40