Second-site suppressors of a cold-sensitive prohead accessory protein of bacteriophage phi X174.
This study describes the isolation of second-site suppressors which correct for the defects associated with cold-sensitive (cs) prohead accessory proteins of bacteriophage phi X174. Five phenotypically different suppressors were isolated. Three of these suppressors confer novel temperature-sensitive (ts) phenotypes. They were unable to complement a ts mutation in gene F which encodes the major coat protein of the phage. All five suppressor mutations confer nucleotide changes in the gene F DNA sequence. These changes define four amino acid sites in the gene F protein. Three suppressor mutations placed into an otherwise wild-type background display a cold resistant phenotype in liquid culture infections when compared to a wild-type phi X174 control.
Fane
B A
BA
Department of Biology, University of California, San Diego, La Jolla 92093.
Hayashi
M
M
D000595:Amino Acid Sequence; D010584:Bacteriophage phi X 174 / Q000235:genetics* / Q000254:growth & development; D003080:Cold Temperature; D016153:Genes, Suppressor / Q000235:genetics* / Q000502:physiology; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D014764:Viral Proteins / Q000097:blood / Q000235:genetics*; D014771:Virion / Q000254:growth & development
D014764:Viral Proteins