Massive cluster impact mass spectrometry: a new desorption method for the analysis of large biomolecules.
A new ion desorption method is described that utilizes a primary beam of massive, multiply charged cluster ions to generate secondary ions of peptides in a glycerol matrix. The massive cluster ion beam is generated via electrohydrodynamic emission using a 1.5 M solution of ammonium acetate in 30% aqueous glycerol. Negative ion spectra of peptides obtained using this technique show greatly decreased relative intensities for fragment ions and 'chemical noise' background when compared to spectra obtained using a xenon atom primary beam. The near absence of fragments derived from radiation damage to the sample solution is attributed to the impact of primary particles with energies less than 1 eV/nucleon.
Mahoney
J F
JF
Phrasor Scientific Inc., Duarte, CA 91010.
Perel
J
J
Ruatta
S A
SA
Martino
P A
PA
Husain
S
S
Lee
T D
TD
D000595:Amino Acid Sequence; D004563:Electrochemistry; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*
D010455:Peptides