Characterization of a candidate bcl-1 gene.
The t(11;14)(q13;q32) translocation has been associated with human B-lymphocytic malignancy. Several examples of this translocation have been cloned, documenting that this abnormality joins the immunoglobulin heavy-chain gene to the bcl-1 locus on chromosome 11. However, the identification of the bcl-1 gene, a putative dominant oncogene, has been elusive. In this work, we have isolated genomic clones covering 120 kb of the bcl-1 locus. Probes from the region of an HpaII-tiny-fragment island identified a candidate bcl-1 gene. cDNAs representing the bcl-1 mRNA were cloned from three cell lines, two with the translocation. The deduced amino acid sequence from these clones showed bcl-1 to be a member of the cyclin gene family. In addition, our analysis of expression of bcl-1 in an extensive panel of human cell lines showed it to be widely expressed except in lymphoid or myeloid lineages. This observation may provide a molecular basis for distinct modes of cell cycle control in different mammalian tissues. Activation of the bcl-1 gene may be oncogenic by directly altering progression through the cell cycle.
Withers
D A
DA
Department of Medicine, University of California, San Francisco.
Harvey
R C
RC
Faust
J B
JB
Melnyk
O
O
Carey
K
K
Meeker
T C
TC
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D019938:Cyclin D1; D016213:Cyclins / Q000235:genetics*; D015870:Gene Expression / Q000502:physiology; D005799:Genes, Dominant / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009857:Oncogenes / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016415:Sequence Alignment; D014178:Translocation, Genetic / Q000235:genetics; D014407:Tumor Cells, Cultured
D016213:Cyclins; D011518:Proto-Oncogene Proteins; D019938:Cyclin D1