Induction by NGF of meiotic maturation of Xenopus oocytes expressing the trk proto-oncogene product.
The effect of nerve growth factor (NGF) was assessed in Xenopus oocytes expressing the human trk proto-oncogene product, p140prototrk. Oocytes injected with trk messenger RNA expressed polypeptides recognized by antibodies to the trk gene product. Exposure of these oocytes to nanomolar amounts of NGF resulted in specific surface binding of 125I-labeled NGF, tyrosine phosphorylation of p140prototrk, and meiotic maturation, as determined by germinal vesicle breakdown and maturation promoting factor (p34cdc2) kinase activation. Thus the trk proto-oncogene product can act as a receptor for NGF in a functionally productive manner.
Nebreda
A R
AR
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
Martin-Zanca
D
D
Kaplan
D R
DR
Parada
L F
LF
Santos
E
E
D000818:Animals; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008540:Meiosis / Q000187:drug effects*; D008845:Microinjections; D009414:Nerve Growth Factors / Q000378:metabolism / Q000494:pharmacology*; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011374:Progesterone / Q000494:pharmacology; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000008:administration & dosage / Q000235:genetics; D020917:Receptor, trkA; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor; D014982:Xenopus laevis
C000715470:MAS1 protein, human; D009414:Nerve Growth Factors; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor; D011374:Progesterone; D011505:Protein-Tyrosine Kinases; D020917:Receptor, trkA