title: Different inhibitory potencies of various ANF-analogues in the isolated aorta from four rodent species.
abstract: Five ANF (atrial natriuretic factor) analogues were tested under similar conditions for their inhibitory potency against noradrenaline induced contractions in the isolated aorta from rabbits, rats, mice and guinea-pigs. The test compounds were: rat ANF 99-126, rat ANF 103-123, [Mpr105-Ala107] rat ANF 105-126-NH2 (PL-058), human ANF 105-126 and human ANF 99-126 oxidized at Met110. The potencies of the test peptides were compared to that of the reference peptide alpha-human ANF 99-126, the potency of which was set to 1. The EC50 values for the reference peptide varied between 2.4 (rabbit) and 6.6 (rat) x 10(-10) M. The highest and lowest relative potencies of the test peptides were: rat ANF 99-126: 2.6 (rat) and 1.4 (guinea-pig); rat ANF 103-123: 1.0 (rat) and 0.005 (rabbit); PL-058: 47 (mouse) and 5 (rabbit); human ANF 105-126: 2.1 (guinea-pig) and 0.5 (rabbit); oxidized human ANF 99-126: 0.3 (guinea-pig) and 0.02 (rat). The species related differences in the relative potencies of the peptides are probably due to structural dissimilarities of the vascular ANF receptors in the different species.
authors.affiliation: Department of Pharmacology, Novo Nordisk, A/S, Bagsvaerd, Denmark.
authors.forename: J
authors.initials: J
authors.lastname: Weis
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D051379:Mice; D009638:Norepinephrine / Q000037:antagonists & inhibitors*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D014665:Vasodilator Agents
chemical_list: D014665:Vasodilator Agents; D009320:Atrial Natriuretic Factor; D009638:Norepinephrine