In vitro and in vivo potentiation of chloroquine against malaria parasites by an enantiomer of amlodipine.
A combination of chloroquine and amlodipine, a derivative of 1,4-dihydropyridine calcium channel blocker, was tested against Plasmodium falciparum in vitro and P. yoelii in mice. The dextrorotary enantiomer of amlodipine, practically devoid of calcium channel blocking action, increased chloroquine accumulation inside the infected mouse erythrocytes and potentiated chloroquine action against the resistant strains of P. falciparum in vitro and P. yoelii in mice. Unlike the racemate, the dextrorotary amlodipine was not toxic to the host animal, even at the highest dose of 250 mg/kg. No potentiating effect was noted in the chloroquine-susceptible strains of P. falciparum. The results of this study indicate that chloroquine potentiation of amlodipine is probably independent of calcium channels and that a combination therapy of the dextrorotary enantiomer of amlodipine and chloroquine might be a potentially useful therapeutic strategy against chloroquine-resistant falciparum malaria.
Deloron
P
P
Institut National de la Santé et de la Recherche Médicale Unité 13, Claude Bernard Hospital, Paris, France.
Basco
L K
LK
Dubois
B
B
Gaudin
C
C
Clavier
F
F
Le Bras
J
J
Verdier
F
F
D017311:Amlodipine; D000818:Animals; D002738:Chloroquine / Q000097:blood / Q000494:pharmacology*; D004357:Drug Synergism; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D016778:Malaria, Falciparum / Q000097:blood / Q000188:drug therapy; D051379:Mice; D009543:Nifedipine / Q000031:analogs & derivatives* / Q000494:pharmacology; D010961:Plasmodium / Q000187:drug effects*; D010963:Plasmodium falciparum / Q000187:drug effects; D013237:Stereoisomerism
D017311:Amlodipine; D002738:Chloroquine; D009543:Nifedipine