Detection of zearalenone in cereal extracts using high-performance liquid chromatography with post-column derivatization.
Post-column derivatization has been used to enhance the fluorescence response of the Fusarium mycotoxins zearalenone and zearalenol when determined by reversed-phase high-performance liquid chromatography. Derivatization is based on reaction with aluminum chloride and this results in a more selective response for these toxins. The method was tested on a number of cereals and animal feeds.
Hetmanski
M T
MT
MAFF, Central Science Laboratory, Slough, Berkshire, UK.
Scudamore
K A
KA
D000535:Aluminum; D000077410:Aluminum Chloride; D017607:Aluminum Compounds; D000821:Animal Feed / Q000032:analysis*; D002712:Chlorides; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry*; D015025:Zearalenone / Q000032:analysis*
D017607:Aluminum Compounds; D002712:Chlorides; D000077410:Aluminum Chloride; D015025:Zearalenone; D000535:Aluminum