Translational control of equine herpesvirus type 1 gene expression.
Translational control mechanisms modulate gene expression in a variety of cellular and viral systems. Using hypertonic conditions to block protein synthesis in vivo, we observed that the synthesis of several major equine herpesvirus type 1 proteins was selectively inhibited. Although sensitivity to hypertonic conditions was graded across a continuum, messages coding for proteins of 203, 130.5, and 31.5 kDa were significantly more resistant to higher salt concentrations in vivo than those coding for polypeptides of 148, 116, and 74 kDa. Similar results were observed in vitro when potassium acetate was used to block initiation. In addition, Northern blot analyses demonstrated that steady-state levels of cellular mRNAs declined beginning at about 6 hr after infection. Taken together, these results indicate that the expression of several major equine herpesvirus type 1 genes was controlled in part at the post-transcriptional level.
Chinchar
V G
VG
Department of Microbiology, University of Mississippi Medical Center, Jackson 39216-4505.
Yu
W
W
Hsu
H S
HS
D000085:Acetates / Q000494:pharmacology; D019342:Acetic Acid; D000818:Animals; D015967:Gene Expression Regulation, Viral / Q000187:drug effects / Q000235:genetics*; D005814:Genes, Viral / Q000187:drug effects; D004861:Herpesvirus 1, Equid / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D006982:Hypertonic Solutions / Q000494:pharmacology; D066298:In Vitro Techniques; D014176:Protein Biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects; D012367:RNA, Viral / Q000187:drug effects / Q000235:genetics; D011817:Rabbits; D014764:Viral Proteins / Q000187:drug effects
D000085:Acetates; D006982:Hypertonic Solutions; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D019342:Acetic Acid