On the functional form of transcortin-recognizing subunit of transcortin membrane receptor.
Complex formation between transcortin and the 20 kDa sialoglycoprotein from the plasma membrane of human decidual endometrium (presumably a transcortin-recognizing subunit of transcortin membrane receptor) was studied using cross-linking reagents. The action of 1,5-difluoro-2,4-dinitrobenzene (DFDNB) on a solution of 125I-labelled 20 kDa sialoglycoprotein and unlabelled transcortin resulted in the formation of two 125I-containing containing species that corresponded to covalently linked complexes of one transcortin molecule and either 2 or 4 molecules of the labeled membrane sialoglycoprotein. Only the latter complex was observed when the endometrium membranes were incubated with [125I]transcortin and treated with DFDNB. This suggests that the functional form of transcortin-recognizing subunit of the membrane receptor is a tetramer.
Krupenko
S A
SA
Institute of Bioorganic Chemistry, Byelorussian Academy of Sciences, Minsk, USSR.
Avvakumov
G V
GV
Strel'chyonok
O A
OA
D002462:Cell Membrane / Q000378:metabolism; D002846:Chromatography, Affinity; D003656:Decidua / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004717:Endometrium / Q000378:metabolism; D005260:Female; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D015843:Serpins; D014156:Transcortin / Q000378:metabolism*
D046911:Macromolecular Substances; D011956:Receptors, Cell Surface; C534935:SERPINA6 protein, human; D015843:Serpins; D014156:Transcortin