Hirudin--a potential stabilizing factor for platelet preservation in transfusion.
The influence of additional hirudin in low doses on platelet and coagulation parameters was studied in platelet concentrates (PC) prepared by cytapheresis using citrate-dextrose solution formula A. The in vitro release of platelet factor 4 and beta-thromboglobulin was markedly lowered and morphological platelet alterations, as assessed by platelet size studies were considerably less accentuated in the presence of hirudin over a 5-day storage period; this suggests a possibly protecting effect on platelet function. No clear activation of the coagulation and fibrinolysis system was evident during PC storage with and without hirudin since thrombin-antithrombin III complexes and D-dimers did not markedly change.
Pindur
G
G
Department of Clinical Haemostaseology and Transfusion Medicine, University of the Saarland, Homburg/Saar, FRG.
Wenzel
E
E
Koehler
M
M
Heiden
M
M
Braun
B
B
D001777:Blood Coagulation / Q000187:drug effects; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D001793:Blood Preservation / Q000379:methods*; D019343:Citric Acid; D005069:Evaluation Studies as Topic; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D005342:Fibrinolysis / Q000187:drug effects; D005947:Glucose / Q000031:analogs & derivatives / Q000494:pharmacology; D006629:Hirudins / Q000494:pharmacology*; D006801:Humans; D010978:Platelet Factor 4 / Q000378:metabolism; D010983:Plateletpheresis; D011994:Recombinant Proteins / Q000494:pharmacology; D012636:Secretory Rate / Q000187:drug effects; D001620:beta-Thromboglobulin / Q000378:metabolism
D005338:Fibrin Fibrinogen Degradation Products; D006629:Hirudins; D011994:Recombinant Proteins; D001620:beta-Thromboglobulin; C036309:fibrin fragment D; C002113:acid citrate dextrose; D019343:Citric Acid; D010978:Platelet Factor 4; D005947:Glucose