Effect of glycosylation inhibitors on binding properties of the Fc gamma receptors from guinea pig peritoneal macrophages.
Guinea pig peritoneal macrophages possess on their surfaces receptors for the Fc region of IgG immunoglobulins (Fc gamma R). The cells treated with the glycosylation inhibitors tunicamycin or monensin interacted with IgG with a higher affinity and showed a lower number of IgG-binding sites in comparison with control cells. This indicates that the interaction of IgG with the macrophage Fc gamma receptor depends on the degree of glycosylation of the cell surface glycoconjugates and that glycosylation of the macrophage Fc gamma receptor is important for the expression of the mature form of the receptor.
Gorczyca
W
W
Department of Immunochemistry, Polish Academy of Sciences, Wrocław, Poland.
Wieczorek
Z
Z
Lisowski
J
J
D000818:Animals; D000943:Antigens, Differentiation / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D001216:Asparagine / Q000378:metabolism; D001665:Binding Sites / Q000187:drug effects; D002241:Carbohydrates / Q000096:biosynthesis; D006031:Glycosylation / Q000187:drug effects; D006168:Guinea Pigs; D007074:Immunoglobulin G / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008985:Monensin / Q000494:pharmacology*; D010529:Peritoneal Cavity; D011817:Rabbits; D011961:Receptors, Fc / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D017452:Receptors, IgG; D014415:Tunicamycin / Q000494:pharmacology*
D000943:Antigens, Differentiation; D002241:Carbohydrates; D007074:Immunoglobulin G; D011961:Receptors, Fc; D017452:Receptors, IgG; D014415:Tunicamycin; D001216:Asparagine; D008985:Monensin