Induction of diabetes by PolyI:C and anti-RT6.1 antibody treatment in DR-BB rats.
To determine whether environmental factors could affect the incidence of diabetes in RT6.1+ lymphocytes-depleted diabetes resistant (DR) BB rats, we tested polyinosinic-polycytidylic acid (Poly I:C), as an immune activator, in conjunction with anti-RT6.1 antibody in DR-BB rats which were bred in a specific pathogen free (SPF) condition. Diabetes was induced by the combined administration of poly I:C and anti-RT6.1 antibody. The use of poly I:C or anti-RT6.1 antibody alone did not cause diabetes. These results suggest that RT6.1+ T lymphocytes regulate autoimmune diabetes and that non-specific immune activation caused by environmental factors plays a key role in inducing diabetes in DR-BB rats.
Nakamura
N
N
First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
Tsutsumi
Y
Y
Kimata
S
S
Sawada
M
M
Hasegawa
G
G
Kitagawa
Y
Y
Nakano
K
K
Kondo
M
M
Nakao
H
H
Makino
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D004357:Drug Synergism; D005856:Germ-Free Life; D006649:Histocompatibility Antigens / Q000276:immunology*; D007518:Isoantibodies / Q000502:physiology; D011070:Poly I-C / Q000276:immunology*; D051381:Rats; D011913:Rats, Inbred BB; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D006649:Histocompatibility Antigens; D007518:Isoantibodies; C079330:RT6.1 antibody; C038353:histocompatibility antigens RT, rat; D011070:Poly I-C