title: On the functional form of transcortin-recognizing subunit of transcortin membrane receptor.
abstract: Complex formation between transcortin and the 20 kDa sialoglycoprotein from the plasma membrane of human decidual endometrium (presumably a transcortin-recognizing subunit of transcortin membrane receptor) was studied using cross-linking reagents. The action of 1,5-difluoro-2,4-dinitrobenzene (DFDNB) on a solution of 125I-labelled 20 kDa sialoglycoprotein and unlabelled transcortin resulted in the formation of two 125I-containing containing species that corresponded to covalently linked complexes of one transcortin molecule and either 2 or 4 molecules of the labeled membrane sialoglycoprotein. Only the latter complex was observed when the endometrium membranes were incubated with [125I]transcortin and treated with DFDNB. This suggests that the functional form of transcortin-recognizing subunit of the membrane receptor is a tetramer.
authors.affiliation: Institute of Bioorganic Chemistry, Byelorussian Academy of Sciences, Minsk, USSR.
authors.forename: S A
authors.initials: SA
authors.lastname: Krupenko
authors.forename: G V
authors.initials: GV
authors.lastname: Avvakumov
authors.forename: O A
authors.initials: OA
authors.lastname: Strel'chyonok
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D002846:Chromatography, Affinity; D003656:Decidua / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004717:Endometrium / Q000378:metabolism; D005260:Female; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D015843:Serpins; D014156:Transcortin / Q000378:metabolism*
chemical_list: D046911:Macromolecular Substances; D011956:Receptors, Cell Surface; C534935:SERPINA6 protein, human; D015843:Serpins; D014156:Transcortin