title: Characterization of human T cells reactive with the Mycoplasma arthritidis-derived superantigen (MAM): generation of a monoclonal antibody against V beta 17, the T cell receptor gene product expressed by a large fraction of MAM-reactive human T cells.
abstract: While all known microbial superantigens are mitogenic for human peripheral blood lymphocytes (PBL), the functional response induced by Mycoplasma arthritidis-derived superantigen (MAM) is unique in that MAM stimulation of PBL consistently results in T cell-dependent B cell activation characterized by polyclonal IgM and IgG production. These immunostimulatory effects of MAM on the humoral arm of the human immune system warranted a more precise characterization of MAM-reactive human T cells. Using an uncloned MAM reactive human T cell line as immunogen, we have generated a monoclonal antibody (mAb) (termed C1) specific for the T cell receptor V beta gene expressed by the major fraction of MAM-reactive human T cells, V beta 17. In addition, a V beta 17- MAM-reactive T cell population exists, assessed by MAM, induced T cell proliferation and cytotoxic T cell activity. mAb C1 will be useful in characterizing the functional properties of V beta 17+ T cells and their potential role in autoimmune disease.
authors.affiliation: Department of Medicine, Hospital for Special Surgery, New York 10021.
authors.forename: S M
authors.initials: SM
authors.lastname: Friedman
authors.forename: M K
authors.initials: MK
authors.lastname: Crow
authors.forename: J R
authors.initials: JR
authors.lastname: Tumang
authors.forename: M
authors.initials: M
authors.lastname: Tumang
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Xu
authors.forename: A S
authors.initials: AS
authors.lastname: Hodtsev
authors.forename: B C
authors.initials: BC
authors.lastname: Cole
authors.forename: D N
authors.initials: DN
authors.lastname: Posnett
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens; D000942:Antigens, Bacterial; D015703:Antigens, CD / Q000096:biosynthesis; D000944:Antigens, Differentiation, B-Lymphocyte / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology; D001483:Base Sequence; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002460:Cell Line; D006801:Humans; D008213:Lymphocyte Activation; D008934:Mitogens / Q000276:immunology*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction / Q000379:methods; D011506:Proteins; D011948:Receptors, Antigen, T-Cell / Q000032:analysis / Q000235:genetics*; D011961:Receptors, Fc / Q000096:biosynthesis; D017455:Receptors, IgE; D018089:Superantigens; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D000942:Antigens, Bacterial; D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D015704:CD4 Antigens; D016827:CD8 Antigens; D008934:Mitogens; C052996:Mycoplasma arthritidis mitogen; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D011506:Proteins; D011948:Receptors, Antigen, T-Cell; D011961:Receptors, Fc; D017455:Receptors, IgE; D018089:Superantigens