[Gastric emptying and metabolic acidosis. I. Study of an experimental model in rats, using an ammonium chloride solution administered through the orogastric route].
The goal of the study was get an experimental model of metabolic acidosis which was appropriated in future gastric emptying studies. For this proposal were utilized 141 female Wistar rats in three stages: in the first (n = 27) was defined the installation, manutention and spontaneous recuperation of the metabolic acidosis produced by the orogastric infusion of a two ml by 100 g of an 0.5 M ammonium chloride solution; in the second (n = 60), it was studied the gastric emptying of the same solution utilized in the first stage and in the last stage (n = 54) it was determined the gastric emptying of the water, for excluding the possibility that the acidificant solution had caused any lesion in the gastric mucosa. As control group were utilized animals fed as a sodium chloride solution in the same concentration and volume that the acidificant solution. The results showed that 6 hours after the infusion, the gastric emptying of two above mentioned meals was complete and there was still a moderate metabolic acidosis in the group with infusion of ammonium chloride solution. Further more, the metabolic acidosis persisted in moderate values until 8 hours after the infusion. Based on these data the interval between 6 and 8 hours after the infusion of the these solutions was considered the best to the further studies about metabolic acidosis and gastric emptying. Finally, the gastric emptying of water, studied in rats with acidosis and controls demonstrated no differences between them. This was interpreted as a clue that the ammonium chloride solution did not modificate the integrity of the gastric mucosa.
Belangero
V M
VM
Departamento de Pediatria e Núcleo de Medicina e Cirurgia Experimental, Faculdade de Ciências Médicas, Universidade Estadual de Campinas-FCM-UNICAMP.
Collares
E F
EF
D000138:Acidosis / Q000097:blood / Q000139:chemically induced / Q000378:metabolism*; D000284:Administration, Oral; D000643:Ammonium Chloride / Q000008:administration & dosage; D000818:Animals; D001784:Blood Gas Analysis; D004195:Disease Models, Animal; D005260:Female; D005746:Gastric Emptying / Q000502:physiology*; D005753:Gastric Mucosa / Q000502:physiology; D006982:Hypertonic Solutions; D007441:Intubation, Gastrointestinal; D051381:Rats; D011919:Rats, Inbred Strains; D012462:Saline Solution, Hypertonic; D013997:Time Factors
D006982:Hypertonic Solutions; D012462:Saline Solution, Hypertonic; D000643:Ammonium Chloride
Esvaziamento gástrico e acidose metabólica. I. Estudo de um modelo experimental em ratos, empregando uma solução de cloreto de amônio por via orogástrica.