title: Expression of XBcad, a novel cadherin, during oogenesis and early development of Xenopus.
abstract: A gastrula cDNA library was screened using a cDNA probe encoding the cytoplasmic domain of uvomorulin, a mouse Ca(2+)-dependent cell adhesion molecule. A Xenopus cDNA clone was isolated, which shares an amino acid sequence identity with uvomorulin of 91% in the transmembrane and 89% in the cytoplasmic domain. A restriction fragment of 397 bp representing the lowest degree of identity to all other known cadherin sequences was used to study the expression pattern of this Xenopus cadherin gene on RNA and protein level. The 397 bp restriction fragment was expressed bacterially as fusion protein, against which polyclonal antibodies were raised. An mRNA of 3.9 kb and a corresponding 125 kDa glycoprotein could be identified. Both molecules are present throughout oogenesis and early embryogenesis. When cleavage starts, the protein becomes integrated into the newly formed membranes. This polypeptide is found at cell membranes of all blastomeres except those at the outer surface of the embryo. Immunoblots and immunohistological analyses of adult organs reveal that this protein is expressed in pituitary gland, lung and kidney. It could not be detected in liver, heart and skeletal muscle. Since this cadherin differs in its tissue distribution from that of U-cadherin and in sequence alignments from ep-cadherin, it was termed XBcad for Xenopus blastomere cadherin.
authors.affiliation: Institut für Molekularbiologie und Biochemie, Berlin, F.R.G.
authors.forename: F
authors.initials: F
authors.lastname: Herzberg
authors.forename: V
authors.initials: V
authors.lastname: Wildermuth
authors.forename: D
authors.initials: D
authors.lastname: Wedlich
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001483:Base Sequence; D001757:Blastomeres / Q000737:chemistry; D015820:Cadherins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D002462:Cell Membrane / Q000502:physiology; D003593:Cytoplasm / Q000737:chemistry; D015342:DNA Probes; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology*; D015151:Immunoblotting; D007150:Immunohistochemistry; D007668:Kidney / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008969:Molecular Sequence Data; D009866:Oogenesis / Q000502:physiology*; D010902:Pituitary Gland / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D014981:Xenopus / Q000502:physiology*
chemical_list: D000906:Antibodies; D015820:Cadherins; D015342:DNA Probes; D012333:RNA, Messenger