Kinetic analysis of regulated myosin ATPase activity using single and limited turnover assays.
Myosin from molluscan adductor muscle is regulated directly by Ca2+ binding. In the absence of Ca2+ the ATPase activity is greatly inhibited. We review the application of transient kinetic methods to this system and show how they can be simple to perform and less ambiguous than steady-state methods.
Ankrett
R J
RJ
Department of Biochemistry, University of Leicester, UK.
Walmsley
A R
AR
Bagshaw
C R
CR
D000818:Animals; D002118:Calcium / Q000378:metabolism; D007700:Kinetics; D008722:Methods; D008974:Mollusca; D009132:Muscles / Q000201:enzymology*; D009218:Myosins / Q000378:metabolism*
D009218:Myosins; D002118:Calcium