Association of NMDA receptor sites and seizures of El mice.
To investigate the possible role of N-methyl-D-aspartate (NMDA) receptors in the seizures of El mice, a genetic animal model of epilepsy, we measured [3H]3-[(+)-2-(carboxypiperazin-4-yl)][1,2-3H]- propyl-1-phosphonic acid (CPP) binding in several brain regions of El and ddY mice. At 22-24 weeks of age, the maximum number of binding sites (Bmax) of [3H]CPP was lower only in the cerebral cortex of both stimulated and unstimulated El mice (El(+) and El(-), respectively) than in that of ddY mice. A reduction in Bmax values of cortical [3H]CPP binding of El mice was detected after the age of 12 weeks. Cortical [3H]CPP binding in El(+) mice decreased further transiently after evoked seizures. No significant change was observed in El(-) mice after postural stimulation. These results suggest that El(+) and El(-) mice share seizure propensity and that activation of NMDA receptors is involved in the seizures of El mice.
Oguro
K
K
Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.
Ito
M
M
Tsuda
H
H
Mutoh
K
K
Shiraishi
H
H
Shirasaka
Y
Y
Mikawa
H
H
D000375:Aging / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000378:metabolism*; D004827:Epilepsy / Q000378:metabolism* / Q000503:physiopathology; D051379:Mice; D008818:Mice, Neurologic Mutants; D011869:Radioligand Assay; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism* / Q000502:physiology
D016194:Receptors, N-Methyl-D-Aspartate