Establishment of idiotype bearing B-lymphocyte clones from a patient with monoclonal gammopathy.
B-lymphocyte clones bearing an idiotypic Ig structure, which could also be found on the serum M-component, have been isolated from peripheral blood of a patient with monoclonal gammopathy (MG) using an immune-rosetting technique and limiting dilution. The majority of the cells had the surface phenotype id+/CD19+/CD20+/CD21+ or id+/CD19+/CD20+/CD21-. Some of the clones originated from spontaneous outgrowth of lymphocytes and some were derived from in vitro Epstein-Barr virus-infected lymphocytes. Three of the B-lymphocyte clones and the patient's bone marrow plasma cells appeared to have clonal identity as shown by the same Ig heavy chain gene rearrangements in Southern blot analysis using a JH probe. The study further supports the notion that the peripheral blood of patients with MG contains B lymphocytes that belong to the tumor clone.
Osterborg
A
A
Department of Oncology (Radiumhemmet), Karolinska Hospital, Stockholm, Sweden.
Steinitz
M
M
Lewin
N
N
Bergenbrant
S
S
Holm
G
G
Lefvert
A K
AK
Mellstedt
H
H
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology*; D015139:Blotting, Southern; D002460:Cell Line; D002999:Clone Cells; D004247:DNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005455:Fluorescent Antibody Technique; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis* / Q000302:isolation & purification; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007130:Immunoglobulin Idiotypes / Q000032:analysis*; D007525:Isoelectric Focusing; D008875:Middle Aged; D010265:Paraproteinemias / Q000235:genetics / Q000276:immunology*; D010641:Phenotype; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D004247:DNA