Co-stimulation of gastrointestinal tumour cell growth by gastrin, transforming growth factor alpha and insulin like growth factor-I.
Epidermal growth factor receptors and insulin like growth factor-I receptors were co-expressed on two gastric and three colorectal tumour cell lines. Previous studies have shown that gastrin receptors were also expressed at a low level or two of these cell lines. Both TGF alpha and IGF-I promoted cell growth in all of the cell lines tested. The cell doubling time of a colorectal cell line was reduced from 48 to 30-34 h. Furthermore the effects of the growth factors were additive. Each growth factor also increased the response of the cells to gastrin, but a combination of both growth factors and gastrin did not further increase growth.
Durrant
L G
LG
Cancer Research Campaign Laboratories, University of Nottingham, UK.
Watson
S A
SA
Hall
A
A
Morris
D L
DL
D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000473:pathology* / Q000648:ultrastructure; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D066246:ErbB Receptors / Q000502:physiology; D005755:Gastrins / Q000494:pharmacology*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008934:Mitogens / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000502:physiology; D017451:Receptors, Somatomedin; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000473:pathology* / Q000648:ultrastructure; D016211:Transforming Growth Factor alpha / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D005755:Gastrins; D008934:Mitogens; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D016211:Transforming Growth Factor alpha; D007334:Insulin-Like Growth Factor I; D066246:ErbB Receptors