title: Biosynthesis of glucose oxidase by producing strain Z-I-C in batch and chemostat culture.
abstract: The biosynthesis of glucose oxidase (GOD) by Penicillium-producing strain Z-I-C in batch and chemostat culture was studied. In a glucose-limited chemostat, the experimental results showed that the maintenance coefficient of this strain was 0.04 g glucose/g dry cell wt/h; yield factor of growth was 0.714 g dry cell wt/g glucose; maximum specific growth rate was 0.385 h and saturation constant Ks was 4.76 g/l. Theoretical optimum dilution rate was 0.260 h-1. Maximum specific enzyme activity was increased from 1.51 x 10(3) mu/mg in batch culture to 2.16 x 10(3) mu/mg in chemostat culture and to 3.11 x 10(3) mu/mg in chemostat culture supplemented in feed medium with 0.02% inducer, alpha-methyl-D-glucose, a 43% or 106% increase, respectively, compared with that of batch culture.
authors.affiliation: Zhejiang Institute of Technology, Hangzhou, China.
authors.forename: W
authors.initials: W
authors.lastname: Pu
authors.forename: L
authors.initials: L
authors.lastname: Yourong
mesh_terms: D003470:Culture Media; D005285:Fermentation; D005949:Glucose Oxidase / Q000096:biosynthesis*; D010407:Penicillium / Q000201:enzymology* / Q000254:growth & development* / Q000378:metabolism
chemical_list: D003470:Culture Media; D005949:Glucose Oxidase