Encephalomyocarditis virus RNA polymerase preparations, with and without RNA helicase activity.
RNA template- and primer-dependent preparations of RNA polymerase were purified from encephalomyocarditis virus-infected Krebs-2 cells, using a three-step chromatographic procedure. The RNA duplex-unwinding activity of these preparations was investigated by two assays, using a partially double-stranded RNA template (encephalomyocarditis virus RNA annealed with a long segment of antisense transcript). Less purified preparations of the polymerase appeared to be able to efficiently displace, in an ATP-dependent and RNA elongation-dependent reaction, the antisense segment from the template. However, upon a more extensive purification, the unwinding activity of the RNA polymerase preparations was lost.
Dmitrieva
T M
TM
A. N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
Norkina
K B
KB
Agol
V I
VI
D002845:Chromatography; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification / Q000378:metabolism*; D004686:Encephalomyocarditis virus / Q000201:enzymology*; D020365:RNA Helicases; D012316:RNA Nucleotidyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism; D013698:Templates, Genetic
D012367:RNA, Viral; D012316:RNA Nucleotidyltransferases; D012321:DNA-Directed RNA Polymerases; D020365:RNA Helicases