Lack of alloantigen-specific cytotoxic T cell activity in the TCR-gamma delta T cell subpopulation of alloantigen-immune chickens.
We examined the in vivo generation of alloantigen-specific cytotoxic T lymphocytes in chicken TCR-alpha beta and TCR-gamma delta T cells. The TCR-alpha beta and TCR-gamma delta subpopulations were separated from spleen of alloantigen-immune chickens using a negative selection method. The separated cells were examined for alloantigen-specific CTL activity in a 51Cr release assay. While negatively selected TCR-alpha beta cells exerted alloantigen-specific CTL activity, no cytotoxic activity could be detected with TCR-gamma delta cells.
Cihak
J
J
Institute of Animal Physiology, University of Munich, FRG.
Merkle
H
H
Lösch
U
U
D000818:Animals; D015703:Antigens, CD; D002645:Chickens; D007114:Immunization; D007519:Isoantigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D015703:Antigens, CD; D007519:Isoantigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta