Effect of dietary essential fatty acid deficiency on Ca(2+)-ATPase activity in microsomal fraction of rat submandibular gland.
1. The effect of dietary essential fatty acid (EFA) deficiency on Ca(2+)-ATPase activity of rat submandibular gland microsomal fraction was studied. 2. The specific activity of Ca(2+)-ATPase per milligram of microsomal protein was depressed about 35% in rats fed the EFA-deficient diet as compared with that in those fed the control diet. 3. Lineweaver-Burk plots for Ca(2+)-ATPase activity showed no significant differences in Km values for Ca2+ and ATP, but the Vmax was decreased in the EFA-deficient rats. 4. The above results suggest that depression of the Ca(2+)-ATPase activity in rats fed the EFA-deficient diet is probably due to the decrease in the Vmax of the enzyme.
Yokoyama
H
H
Department of Biochemistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Matsukawa
R
R
D000818:Animals; D001835:Body Weight; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D004041:Dietary Fats / Q000008:administration & dosage; D005228:Fatty Acids, Essential / Q000172:deficiency*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D009929:Organ Size; D051381:Rats; D013363:Submandibular Gland / Q000033:anatomy & histology / Q000201:enzymology*
D004041:Dietary Fats; D005228:Fatty Acids, Essential; D000252:Calcium-Transporting ATPases