Molecular cloning of cDNA encoding adipogenesis inhibitory factor and identity with interleukin-11.
A cDNA encoding a novel adipogenesis inhibitory factor (AGIF) that inhibits the process of adipogenesis in mouse 3T3-L1 preadipocytes was cloned from a cDNA library of the human bone marrow-derived stromal cell line KM-102. The cloned cDNA contains an open reading frame coding for an AGIF precursor of 199 amino acids. Analysis of the sequence of this cDNA revealed identity of this factor with a recently reported novel cytokine, designated interleukin-11 (IL-11). AGIF/IL-11 may play an important role in stromal cell-associated hematopoeisis through its regulatory action on adipocyte differentiation in the bone marrow microenvironment.
Kawashima
I
I
Bioscience Research Laboratories, Sankyo Co. Ltd., Tokyo, Japan.
Ohsumi
J
J
Mita-Honjo
K
K
Shimoda-Takano
K
K
Ishikawa
H
H
Sakakibara
S
S
Miyadai
K
K
Takiguchi
Y
Y
D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D001853:Bone Marrow; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D015723:Gene Library; D006801:Humans; D017370:Interleukin-11; D007378:Interleukins / Q000235:genetics* / Q000494:pharmacology; D008071:Lipoprotein Lipase / Q000037:antagonists & inhibitors; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D014162:Transfection
D017370:Interleukin-11; D007378:Interleukins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D004247:DNA; D008071:Lipoprotein Lipase