title: Conservation of cation-transporting ATPase genes in Leishmania.
abstract: DNA fragments isolated from Leishmania donovani ATPase genes were used to analyze the organization and expression of cation transporting ATPase genes in L. donovani, Leishmania tropica, Leishmania mexicana, Leishmania braziliensis, Trypanosoma brucei and Trypanosoma cruzi. The ATPase loci in all Leishmania species contained a tandem pair of ATPase genes arranged in head-to-tail orientation and separated by approximately 2 kb. No restriction site polymorphisms were detected in the internal portions of the Leishmania ATPase genes which contain domains conserved between the L. donovani and other eukaryotic plasma membrane ATPases. The ATPase locus of each of the four Leishmania species was mapped to a single small chromosome of approximately 750 kb. The ATPase locus of L. mexicana was differentially expressed. Promastigotes in exponential growth contained abundant transcripts from the upstream ATPase gene, while transcripts from the downstream gene were relatively scarce. Transcripts from the downstream ATPase gene increased in abundance in promastigotes allowed to reach the stationary phase of growth and were most abundant in amastigotes. The two trypanosome species were found to contain DNA fragments that hybridized strongly to the Leishmania ATPase gene.
authors.affiliation: Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati Medical Center, OH 45267-0524.
authors.forename: J C
authors.initials: JC
authors.lastname: Meade
authors.forename: G H
authors.initials: GH
authors.lastname: Coombs
authors.forename: J C
authors.initials: JC
authors.lastname: Mottram
authors.forename: P E
authors.initials: PE
authors.lastname: Steele
authors.forename: J R
authors.initials: JR
authors.lastname: Stringer
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000818:Animals; D016054:DNA, Protozoan / Q000737:chemistry; D015870:Gene Expression; D007891:Leishmania / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D007893:Leishmania donovani / Q000201:enzymology / Q000235:genetics*; D016053:RNA, Protozoan / Q000378:metabolism; D015183:Restriction Mapping
chemical_list: D016054:DNA, Protozoan; D016053:RNA, Protozoan; D000251:Adenosine Triphosphatases