title: Autogeneic bone marrow and porous biphasic calcium phosphate ceramic for segmental bone defects in the canine ulna.
abstract: The purpose of this study was to evaluate a porous biphasic hydroxyapatite-calcium phosphate ceramic as a modifier and extender of an autogeneic marrow graft for filling a 2.5-cm segmental bony defect. Twenty adult mongrel dogs were surgically treated to create diaphyseal defects in the left ulnae. The defects were (1) filled with autogeneic bone marrow mixed with granular hydroxyapatite-tricalcium phosphate ceramic (granular ceramic); (2) grafted with a solid block of ceramic soaked in autogeneic bone marrow (block ceramic); (3) received no graft (no implant); or (4) were grafted with autogeneic bone marrow alone (bone marrow). All animals were followed clinically and roentgenographically for 24 weeks and then killed. Repair of diaphyseal defects with the block ceramic led to three solid unions and three fibrous unions; with the granular ceramic implants and marrow, the defects of five dogs formed solid unions, and one progressed to a fibrous union. Defects in all five dogs grafted with autogeneic bone marrow united. The three dogs with no implant formed nonunions. Histology showed normal marrow and only a light immune reaction. Complete bridging of the defect in the dogs treated with the granular ceramic occurred significantly earlier than bridging in the dogs grafted with bone marrow alone. Histomorphometry, performed on the block ceramic implants indicated active resorption of ceramic. Clinically, addition of ceramic to a marrow graft improved the handling characteristics of the graft material and accelerated healing according to roentgenographic evaluation.
authors.affiliation: Department of Orthopedic Surgery, University of California, Davis, Sacramento 95817.
authors.forename: R E
authors.initials: RE
authors.lastname: Grundel
authors.forename: M W
authors.initials: MW
authors.lastname: Chapman
authors.forename: T
authors.initials: T
authors.lastname: Yee
authors.forename: D C
authors.initials: DC
authors.lastname: Moore
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage; D001696:Biomechanical Phenomena; D016026:Bone Marrow Transplantation; D002130:Calcium Phosphates / Q000008:administration & dosage*; D002516:Ceramics; D004285:Dogs; D017886:Durapatite; D006882:Hydroxyapatites / Q000008:administration & dosage; D014182:Transplantation, Autologous; D014457:Ulna / Q000293:injuries / Q000502:physiology / Q000601:surgery*; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D006882:Hydroxyapatites; C485817:beta-tricalcium phosphate; D017886:Durapatite; C018392:tricalcium phosphate