Identification of a zinc finger protein that inhibits IL-2 gene expression.
Transient activation of the interleukin-2 (IL-2) gene after antigen recognition by T lymphocytes is crucial for subsequent T cell proliferation and differentiation. Several IL-2 gene regulatory elements and binding factors necessary for activation of the IL-2 gene have been defined. However, little is known about negative regulation of IL-2 expression, which is likely to be important in the rapid shut-off of IL-2 transcription. A nucleotide sequence element (NRE-A) that negatively regulates IL-2 expression has been identified within the IL-2 gene. T cell nuclear extracts contained an NRE-A binding activity. A complementary DNA was isolated that encodes a zinc finger-containing protein that suppressed IL-2 gene expression. The observation of negative regulation of the immunoglobulin heavy chain gene enhancer by an element similar to NRE-A suggests that related proteins may regulate multiple immune response genes.
Williams
T M
TM
Department of Pathology, School of Medicine, University of New Mexico, Albuquerque 87131.
Moolten
D
D
Burlein
J
J
Romano
J
J
Bhaerman
R
R
Godillot
A
A
Mellon
M
M
Rauscher
F J
FJ
Kant
J A
JA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015342:DNA Probes; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D005803:Genes, Immunoglobulin; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007376:Interleukin-2 / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D013601:T-Lymphocytes / Q000276:immunology*; D014158:Transcription, Genetic; D016335:Zinc Fingers / Q000235:genetics* / Q000502:physiology
D015342:DNA Probes; D007143:Immunoglobulin Heavy Chains; D007376:Interleukin-2; D009838:Oligodeoxyribonucleotides