title: Influence of repeated lyophilization on the survival of Deinococcus proteolyticus, Micrococcus luteus and Escherichia coli.
abstract: Repeated lyophilization of Deinococcus proteolyticus, Micrococcus luteus and Escherichia coli cells results in a successive decrease of their survival. The survival curve is exponential with E. coli and M. luteus, and sigmoidal with a broad shoulder with D. proteolyticus both after repeated lyophilization and after UV- or gamma-irradiation. When cells were subjected to gamma-irradiation after a 20-fold freeze-drying, the corresponding survival curve became exponential without the shoulder. Hence we assume that irradiation and repeated lyophilization afflict the same cellular structures and/or functions.
authors.affiliation: Biophysical Institute, Czechoslovak Academy of Sciences, Brno.
authors.forename: L
authors.initials: L
authors.lastname: Rýznar
authors.forename: V
authors.initials: V
authors.lastname: Drásil
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics / Q000528:radiation effects; D004926:Escherichia coli / Q000166:cytology* / Q000235:genetics / Q000528:radiation effects; D005612:Freeze Drying; D005615:Freezing; D005720:Gamma Rays; D008837:Micrococcus / Q000166:cytology* / Q000235:genetics / Q000528:radiation effects; D016982:Micrococcus luteus / Q000166:cytology* / Q000235:genetics / Q000528:radiation effects; D011836:Radiation Tolerance
chemical_list: D004269:DNA, Bacterial