Lysophosphatidylcholine induces mast cell secretion and protein kinase C activation.
Lysophosphatidylcholine (lyso-PC), a natural product of phospholipase A2 activity, induced the secretion of both granule-associated beta-hexosaminidase and newly generated leukotriene C4 from mouse bone marrow-derived mast cells. Micromolar concentrations of lyso-PC potentiated the release of beta-hexosaminidase induced by specific antigen but not the calcium ionophore, A23187. Exogenous adenosine was relatively ineffective in enhancing beta-hexosaminidase release from cells challenged with lyso PC. Lyso-PC caused a marked increase in intracellular free-calcium levels and induced the activation of protein kinase C (PKC). These effects could not be abrogated by a prolonged preincubation with pertussis toxin. Staurosporine, an inhibitor of PKC, partially inhibited the abilities of antigen and A23187 to induce beta-hexosaminidase release but was ineffective when lyso-PC was the secretagogue. Lyso-PC appears to activate mast cell PKC, but its ability to stimulate mast cell mediator release appears to be related to its ability to elevate intracellular free calcium concentrations.
Marquardt
D L
DL
Department of Medicine, University of California-San Diego School of Medicine.
Walker
L L
LL
D000241:Adenosine / Q000494:pharmacology; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D001854:Bone Marrow Cells; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D013189:SRS-A / Q000378:metabolism; D019311:Staurosporine; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D000470:Alkaloids; D008244:Lysophosphatidylcholines; D013189:SRS-A; D000001:Calcimycin; D011493:Protein Kinase C; D001619:beta-N-Acetylhexosaminidases; D019311:Staurosporine; D000241:Adenosine; D002118:Calcium