The sensitivities of SV40-transformed human fibroblasts to monofunctional and DNA-crosslinking alkylating agents.
4 repair-deficient (Mer-) and 2 repair-proficient (Mer+) lines of SV40-transformed human fibroblasts were assayed for colony-forming ability after treatment with MNNG, methyl methanesulfonate (MMS), 1,3-bis-(2-chloroethyl)-1-nitrosourea (BCNU), and 1-(2-chloroethyl)-3-(2-hydroxyethyl)-1-nitrosourea (HECNU). The sensitivities to MMS, BCNU and HECNU of these SV40-transformed lines were similar to those of comparably treated human tumor cells observed previously. However, unlike human tumor lines, whose post-MNNG survival is strongly dependent upon Mer phenotype, SV40-transformed lines showed a lack of dependence of post-MNNG colony-forming ability on Mer phenotype. No differences in glutathione levels that might explain these differences were detected. The amounts of SV40-specific DNA and RNA among the lines were found to vary widely, but no correlation with Mer phenotype was found.
Miyakoshi
J
J
Department of Medicine, Cross Cancer Institute, Edmonton, Alb., Canada.
Scudiero
D A
DA
Allalunis-Turner
J
J
Day
R S
RS
D002330:Carmustine / Q000633:toxicity*; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D004260:DNA Repair; D004279:DNA, Viral / Q000032:analysis; D005347:Fibroblasts; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D008741:Methyl Methanesulfonate / Q000633:toxicity*; D008769:Methylnitronitrosoguanidine / Q000633:toxicity*; D009153:Mutagens; D009154:Mutation; D009607:Nitrosourea Compounds / Q000633:toxicity*; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics
D004279:DNA, Viral; D009153:Mutagens; D009607:Nitrosourea Compounds; D012333:RNA, Messenger; D012367:RNA, Viral; D008769:Methylnitronitrosoguanidine; D008741:Methyl Methanesulfonate; D005978:Glutathione; C013876:elmustine; D002330:Carmustine