Electrophoretical analysis of serum ALP isoenzymes in neonatal calves.
Alkaline phosphatase (ALP) isoenzymes obtained from sera of fetus and neonatal calves were examined by electrophoretical analysis using cellulose acetate membrane. A band at Rf 0.45 was detected in the fetal sera. Two bands at Rf 0.53 and Rf 0.31 were observed in the sera of calves 60 days after birth. By immunoelectrophoretical analysis with the anti-kidney ALP in agarose, a precipitin line appeared from the sera of calves at 4.5 hours after birth. No precipitin line was observed from the fetal sera. Results showed that kidney ALP isoenzymes are present in the serum just after birth in cattle.
Miyazawa
K
K
Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.
Tomoda
I
I
D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D000831:Animals, Newborn / Q000097:blood*; D002417:Cattle / Q000097:blood*; D004588:Electrophoresis, Cellulose Acetate; D005312:Fetal Blood / Q000201:enzymology*; D007122:Immunoelectrophoresis; D007527:Isoenzymes / Q000097:blood*
D007527:Isoenzymes; D000469:Alkaline Phosphatase