title: Resistance to fecal shedding of salmonellae in pigs and chickens vaccinated with an aromatic-dependent mutant of Salmonella typhimurium.
abstract: The purpose of this study was to evaluate the effectiveness of an aromatic-dependent mutant of Salmonella typhimurium as a parenteral vaccine for prevention of fecal shedding of Salmonella spp. Pigs and chickens were vaccinated IM, with 1 x 10(9) and 1 x 10(8) organisms, respectively, followed by a second identical vaccination 2 weeks later. Salmonella organisms were not detected by analysis of fecal or cloacal swab specimens from any animal after vaccination. Deleterious side effects were not noticed after vaccination. Pigs were challenge-inoculated PO with 1 x 10(12) virulent S typhimurium 1 week after the second vaccination. Chickens were challenge-inoculated PO with 3 x 10(8) organisms of either S enteritidis or the virulent parent strain of S typhimurium 3 weeks after the second vaccination. Vaccinated pigs shed Salmonella spp significantly less frequently than did nonvaccinated pigs. Vaccinated chickens challenge-inoculated with either S enteritidis or S typhimurium also shed Salmonella less frequently than the corresponding nonvaccinated control birds; however, the difference was not significant.
authors.affiliation: Department of Veterinary Microbiology and Immunology, Ontario Veterinary College, University of Guelph, Canada.
authors.forename: J S
authors.initials: JS
authors.lastname: Lumsden
authors.forename: B N
authors.initials: BN
authors.lastname: Wilkie
authors.forename: R C
authors.initials: RC
authors.lastname: Clarke
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines; D002353:Carrier State / Q000517:prevention & control / Q000662:veterinary; D002645:Chickens; D002988:Cloaca / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D008297:Male; D011201:Poultry Diseases / Q000517:prevention & control*; D012475:Salmonella / Q000302:isolation & purification; D012481:Salmonella Infections, Animal / Q000517:prevention & control*; D012477:Salmonella enteritidis / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology* / Q000302:isolation & purification; D012692:Serial Passage; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary; D014614:Vaccines, Synthetic
chemical_list: D001428:Bacterial Vaccines; D014614:Vaccines, Synthetic