Metal binding and DNA recognition in transcription factor Sp1.
DNA binding domain of Sp1 encompassing three Cys2His2-type Zn-finger motifs is cloned and expressed in E.coli. The Sp1 fragment shows metal-dependent folding and DNA binding. The Zn(II)-induced folding of the three fingers is probably cooperative. Release of one equivalent of Zn decreases but does not abolish DNA binding activity of Sp1.
Kuwahara
J
J
Institute for Chemical Research, Kyoto University, Japan.
Futamura
M
M
Sogo
N
N
Yonezawa
A
A
Sugiura
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D008969:Molecular Sequence Data; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*; D016335:Zinc Fingers
D016329:Sp1 Transcription Factor; D004247:DNA; D015032:Zinc