A protein kinase is present in a complex with adenovirus E1A proteins.
A kinase activity can be immunoprecipitated in a complex that includes adenovirus E1A proteins. In vitro, this activity phosphorylated other E1A-associated proteins, as well as added E1A and histone H1 proteins. The E1A-associated kinase activity was cleared from extracts with an antibody to cyclin A, but not with antibody to cyclin B. The formation of a complex that included the kinase activity required amino acids 30-60 and 122-129 on the E1A proteins, sequences needed for association of E1A proteins with cyclin A and the retinoblastoma protein and implicated in control of cell growth. The complex of E1A-associated proteins included a 33-kDa ATP-binding protein, similar in size to a cyclin A-associated cdc2 kinase family member. Sucrose gradient analysis revealed two distinct E1A-containing complexes with the kinase activity. We suggest that E1A proteins may affect cellular proliferation by interacting with a member of the cdc2 kinase family and thereby influencing its activity.
Kleinberger
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Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, NJ 08544-1014.
Shenk
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D017456:Adenovirus Early Proteins; D000345:Affinity Labels; D002460:Cell Line; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D016213:Cyclins / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D046911:Macromolecular Substances; D008970:Molecular Weight; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*
D017456:Adenovirus Early Proteins; D000345:Affinity Labels; D016213:Cyclins; D046911:Macromolecular Substances; D009856:Oncogene Proteins, Viral; D011494:Protein Kinases