title: Complement activation by vascular sutures both alone and in combination with synthetic vascular prostheses.
abstract: Polymer surfaces activate complement pathways resulting in platelet and leucocyte deposition as well as possible release of growth factors. A consequence of these interactions may be early graft failure or intimal hyperplasia leading to late graft failure. C5a generation in human plasma by vascular sutures, both alone and in combination with synthetic vascular prostheses was measured by radioimmunoassay to determine the influence of suture materials on C5a activation. Prolene and ePTFE suture material caused significant activation of C5a (p less than 0.01), while Novafil did not. Both Dacron and ePTFE graft material caused significant activation (p less than 0.01) of C5a. The addition of the suture materials to the ePTFE did not increase the C5a levels above the ePTFE material alone. In contrast, the addition of either Prolene or Novafil suture to Dacron material elevated C5a levels significantly over Dacron alone (p less than 0.01). The combination of Dacron material with ePTFE suture did not increase C5a levels over Dacron alone. The pattern of C5a activation by Prolene, ePTFE and Novafil sutures parallels the relative degree of in-vivo platelet accumulation on these suture materials as previously reported by our group. Since these experiments demonstrate that vascular suture material influences human complement activation, it may be that this interaction contributes to either early or late graft failure by enhancing platelet reactivity or neointimal proliferation, respectively.
authors.affiliation: Department of Surgery, Tufts University-New England Medical Center, Boston.
authors.forename: J E
authors.initials: JE
authors.lastname: Coleman
authors.forename: C S
authors.initials: CS
authors.lastname: McEnroe
authors.forename: J A
authors.initials: JA
authors.lastname: Gelfand
authors.forename: R J
authors.initials: RJ
authors.lastname: Connolly
authors.forename: A D
authors.initials: AD
authors.lastname: Callow
mesh_terms: D001807:Blood Vessel Prosthesis; D003167:Complement Activation / Q000187:drug effects*; D015936:Complement C5a / Q000187:drug effects*; D003170:Complement Pathway, Alternative / Q000187:drug effects; D003171:Complement Pathway, Classical / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D011091:Polyesters / Q000494:pharmacology*; D011093:Polyethylene Terephthalates / Q000494:pharmacology*; D011126:Polypropylenes / Q000494:pharmacology*; D011138:Polytetrafluoroethylene / Q000494:pharmacology*; D013537:Sutures
chemical_list: D011091:Polyesters; D011093:Polyethylene Terephthalates; D011126:Polypropylenes; D015936:Complement C5a; D011138:Polytetrafluoroethylene; C048850:polybutester