title: Studies on the N-terminal sequences of lectins isolated from the seeds of Butea frondosa.
abstract: Two lectin fractions (FI and FII) were obtained from seeds of Butea frondosa by affinity chromatography on a sorbent of macroporous glass coupled to the disaccharide alpha-D-GalNAc-(1----3)-beta-D-Gal. Both of these fractions, although different in their sugar specificity, were found on SDS-PAGE to consist of two polypeptide chains of 33 kDa and 35 kDa. In the native state the subunits associated to form a 250 kDa complex, possibly comprising four molecules of the 33 kDa polypeptide and four molecules of the 35 kDa polypeptide. The presence of a faint 70 kDa band when the 250 kDa complex was subjected to SDS-PAGE may indicate the existence of a sequential mechanism of aggregation. N-terminal amino acid sequence analysis revealed extensive homology between these lectins and those of other Leguminosae.
authors.affiliation: M M Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
authors.forename: S
authors.initials: S
authors.lastname: Padmanabhan
authors.forename: V V
authors.initials: VV
authors.lastname: Demin
authors.forename: I N
authors.initials: IN
authors.lastname: Telezhinskaya
authors.forename: E V
authors.initials: EV
authors.lastname: Zaitseva
authors.forename: T B
authors.initials: TB
authors.lastname: Golubeva
authors.lastname: Chertov OYu
mesh_terms: D000595:Amino Acid Sequence; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D037121:Plant Lectins; D010944:Plants / Q000737:chemistry* / Q000196:embryology; D012639:Seeds / Q000737:chemistry*
chemical_list: D037102:Lectins; D037121:Plant Lectins