Distribution and characterization of immunoreactive porcine C-type natriuretic peptide.
C-type natriuretic peptide (CNP) is a new member of the natriuretic peptide family recently identified in porcine brain (1). We raised an antiserum against porcine CNP and set up a radioimmunoassay (RIA) for CNP. Using this RIA system, distribution of immunoreactive (ir-) CNP in porcine tissue was measured and compared with that of ir-atrial natriuretic peptide (ANP) and ir-brain natriuretic peptide (BNP). Tissue concentration of ir-CNP in brain was the highest of the three natriuretic peptides at about 0.79 pmol/g wet wt. CNP was present in medulla-pons in high concentration, with a significant concentration detected in cerebellum. In contrast, ir-CNP was not detected in peripheral tissue, including heart, in a significant concentration. These data demonstrated sharp contrasts in the distribution of the three natriuretic peptides, suggesting that CNP is a natriuretic peptide functioning in the central nervous system.
Ueda
S
S
Department of Biochemistry, Miyazaki Medical College, Japan.
Minamino
N
N
Aburaya
M
M
Kangawa
K
K
Matsukura
S
S
Matsuo
H
H
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis; D001923:Brain Chemistry; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D020098:Natriuretic Peptide, C-Type; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000276:immunology; D009928:Organ Specificity; D011863:Radioimmunoassay; D013116:Spinal Cord / Q000737:chemistry*; D013552:Swine
D007106:Immune Sera; D009419:Nerve Tissue Proteins; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor