Interleukin-6 stimulates motility of vascular endothelium.
Interleukin-6 (IL-6) is a cytokine which regulates host response to injury. Various preparations of recombinant human IL-6 stimulated migration of bovine brain and bovine aortic endothelial cells, with maximal responses at 100-600 ng/ml. The migration response was inhibited by anti-IL-6 monoclonal antibody. IL-6 also inhibited endothelial cell proliferation in a dose-dependent fashion. Combinations of IL-6 and tumor necrosis factor induced additive stimulation of migration. Studies with inhibitors and stimulators of various metabolic processes suggest that IL-6-induced motility: 1) does not require a pertussis toxin-sensitive G-protein, protein kinase C, or DNA synthesis; and 2) is regulated differently from the motility induced by scatter factor. A possible role for IL-6 in the regulation of physiologic angiogenesis is discussed.
Rosen
E M
EM
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT 06510.
Liu
D
D
Setter
E
E
Bhargava
M
M
Goldberg
I D
ID
D000818:Animals; D001011:Aorta; D016466:CHO Cells; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation; D006224:Cricetinae; D016207:Cytokines / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D015850:Interleukin-6 / Q000235:genetics / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014162:Transfection
D016207:Cytokines; D015850:Interleukin-6; D011994:Recombinant Proteins