title: Neurons in raphe nuclei pontis and magnus have branching axons that project to medial preoptic area and cervical spinal cord. A fluorescent retrograde double labeling study in the rat.
abstract: In this study, we utilized a double retrograde axonal tracing technique to investigate the possible existence of collateralized axonal projections from raphe nuclei 'pontis' and 'magnus' to both medial preoptic area (MPA) and cervical spinal cord (C1-C2). Following microinjections of fluorescent tracers Fast blue (FB) and Diamidino yellow (DY) within MPA and C1-C2, substantial numbers of FB and DY single-labeled neurons, as well as FB-DY double-labeled neurons have been found within raphe nuclei 'pontis' and 'magnus'.
authors.affiliation: Chair of General Physiology, Institute of Human Physiology, Catania, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Leanza
authors.forename: R
authors.initials: R
authors.lastname: Pellitteri
authors.forename: A
authors.initials: A
authors.lastname: Russo
authors.forename: S
authors.initials: S
authors.lastname: Stanzani
mesh_terms: D000578:Amidines; D000818:Animals; D005456:Fluorescent Dyes; D008297:Male; D008856:Microscopy, Fluorescence; D009333:Neck; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure; D011301:Preoptic Area / Q000502:physiology*; D011903:Raphe Nuclei / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000502:physiology*; D009435:Synaptic Transmission
chemical_list: D000578:Amidines; D005456:Fluorescent Dyes; C031455:diamidino compound 253-50; C037945:diamidino yellow