title: Neutrophils-induced increase of adenosine triphosphate depletion in rat neonatal cardiac myocytes with impaired energy metabolism.
abstract: Isolated, cultured rat neonatal cardiac myocytes were placed in medium supplemented with mitochondrial respiratory inhibitor potassium cyanide which caused a rapid adenosine triphosphate (ATP) depletion. These myocytes with the impaired energy metabolism ("hypoxia-like state") were exposed to unstimulated human neutrophils. Effect of human neutrophils on the myocytes in the "hypoxia-like state" was quantified as a total change in the amount of ATP in cardiac cells. After 5 hours of incubation of neutrophils with the myocytes in the "hypoxia-like state" an additional decrease (of 50 per cent) in ATP content was observed. Since catalase (which destroys hydrogen peroxide) prevented the further decline in ATP level in the myocytes with impaired energy metabolism, it seem that hydrogen peroxide and possibly their products are responsible for this effect. These results suggest that unstimulated human neutrophils after activation by the contact with injured cardiac cells caused further decrease of ATP level in target cells.
authors.affiliation: Department of Biochemistry, Institute of Rheumatology, Warsaw, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Gajewski
authors.forename: H
authors.initials: H
authors.lastname: Laskowska-Bozek
authors.forename: S
authors.initials: S
authors.lastname: Maśliński
authors.forename: J
authors.initials: J
authors.lastname: Ryzewski
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D002478:Cells, Cultured; D004734:Energy Metabolism; D006801:Humans; D009206:Myocardium / Q000378:metabolism*; D009504:Neutrophils / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000255:Adenosine Triphosphate