title: Physical mapping of the human genome by pulsed field gel analysis.
abstract: Detailed physical maps of large regions of the human genome are important for locating and cloning genes responsible for human hereditary diseases, as well as for obtaining a more detailed understanding of chromosome structure and evolution. Pulsed field gel electrophoresis provides one method for generating physical maps of non-methylated rare restriction endonuclease sites. This review summarizes recent progress in the isolation of region-specific mapping probes and in their application for the physical mapping of selected regions of the human genome.
authors.affiliation: Molecular Genetics Laboratory, Salk Institute for Biological Studies, San Diego, California 92138.
authors.forename: G A
authors.initials: GA
authors.lastname: Evans
mesh_terms: D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D004247:DNA / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D030342:Genetic Diseases, Inborn / Q000235:genetics; D008040:Genetic Linkage; D016045:Human Genome Project; D006801:Humans; D006822:Hybrid Cells; D008285:Major Histocompatibility Complex; D009693:Nucleic Acid Hybridization; D012377:Rodentia
chemical_list: D004247:DNA