title: Enzyme-cytochemical study of small ganglion cells in experimental thiamine deficiency: concerning the pain mechanism.
abstract: In an effort to clarify the mechanism of pain accompanying thiamine deficiency, thiamine monophosphatase (TMPase) activity was demonstrated, by means of electron microscopic cytochemistry, in small ganglion cells in pyrithiamine-treated thiamine-deficient rats. TMPase activity was seen to be present in the reticular part of the Golgi complex and vesicle-like bodies around the trans side of the Golgi complex. By the 10th day after the initial pyrithiamine treatment, a significant increase in the number of TMPase-positive vesicle-like body was observed. These results indicate that thiamine deficiency causes a disturbance in the transportation and/or production of TMPase, which is an integral part of the synaptic transmission of the nociception impulses.
authors.affiliation: 2nd Department of Anatomy, Toho University School of Medicine, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tomiyasu
authors.forename: K
authors.initials: K
authors.lastname: Inomata
mesh_terms: D000818:Animals; D005727:Ganglia, Spinal / Q000201:enzymology / Q000473:pathology*; D006651:Histocytochemistry / Q000379:methods; D008297:Male; D008854:Microscopy, Electron; D010146:Pain / Q000503:physiopathology; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis*; D011745:Pyrithiamine; D051381:Rats; D011919:Rats, Inbred Strains; D013832:Thiamine Deficiency / Q000139:chemically induced / Q000201:enzymology / Q000503:physiopathology*
chemical_list: D011745:Pyrithiamine; C022734:thiamine monophosphatase; D010744:Phosphoric Monoester Hydrolases