title: Effect of omeprazole on gene expression in canine gastric parietal cells.
abstract: Stimulation of gastric parietal cells by carbachol induces coordinate expression of the genes for two enzymes involved in the process of acid secretion, H(+)-K(+)-ATPase and carbonic anhydrase II (CA II). The basis of this coordinate expression was examined in experiments using parietal cells that had been pretreated with omeprazole. We observed a twofold increase in the steady-state mRNA levels of both H(+)-K(+)-ATPase and CA II after cells were treated with the inhibitor. The induction of CA II mRNA by carbachol followed the same kinetics in omeprazole-pretreated cells as in those that were not pretreated, suggesting that the induction of CA II gene expression by carbachol was not dependent on activation of the gastric H(+)-K(+)-ATPase. In addition, carbachol stimulation of omeprazole-pretreated cells resulted in an induction of one or more larger mRNA species that hybridized with the H(+)-K(+)-ATPase probe. The observation that carbachol-induced increases in steady-state levels of beta-actin mRNA in parietal cells could be inhibited by omeprazole pretreatment suggests a possible linkage between increased beta-actin gene expression and the process of acid secretion.
authors.affiliation: Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109-0682.
authors.forename: V W
authors.initials: VW
authors.lastname: Campbell
authors.forename: T
authors.initials: T
authors.lastname: Yamada
mesh_terms: D000199:Actins / Q000235:genetics; D000251:Adenosine Triphosphatases / Q000235:genetics*; D000818:Animals; D015152:Blotting, Northern; D002256:Carbonic Anhydrases / Q000235:genetics*; D015342:DNA Probes; D004285:Dogs; D015870:Gene Expression / Q000187:drug effects; D017506:H(+)-K(+)-Exchanging ATPase; D066298:In Vitro Techniques; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D009853:Omeprazole / Q000494:pharmacology*; D010295:Parietal Cells, Gastric / Q000187:drug effects / Q000201:enzymology*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000199:Actins; D015342:DNA Probes; D012333:RNA, Messenger; D000251:Adenosine Triphosphatases; D017506:H(+)-K(+)-Exchanging ATPase; D002256:Carbonic Anhydrases; D009853:Omeprazole