Down-regulation of prostaglandin E2 receptors in regenerating rat liver and its physiological significance.
The properties of prostaglandin (PG) E2 receptors in regenerating liver were studied using rat hepatocytes in primary culture. The control cells possessed stereo-specific PGE2 receptors with Bmax and Kd values, at 4 degrees C, of 526 fmol/mg protein and 6.5 nM respectively. In cells from regenerating liver after 70% hepatectomy, Bmax was reduced to 42-43% that of the controls; Kd did not change. Administration of indomethacin before surgery prevented Bmax reduction. These results indicate that PGE2, produced during the regeneration process, evoked cellular events and regulated the density of its receptors.
Hashimoto
N
N
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Watanabe
T
T
Ikeda
Y
Y
Toda
G
G
Yamada
H
H
Yoshikawa
Y
Y
Mitsui
H
H
Kurokawa
K
K
D000818:Animals; D001667:Binding, Competitive; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D007213:Indomethacin / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008115:Liver Regeneration; D008297:Male; D011453:Prostaglandins / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011972:Receptor, Insulin / Q000378:metabolism; D011982:Receptors, Prostaglandin / Q000187:drug effects / Q000502:physiology*; D018078:Receptors, Prostaglandin E
D007328:Insulin; D011453:Prostaglandins; D011982:Receptors, Prostaglandin; D018078:Receptors, Prostaglandin E; D011972:Receptor, Insulin; D015232:Dinoprostone; D007213:Indomethacin