title: Clonal variation within a mucosal isolate derived from a patient with Leishmania (Viannia) braziliensis infection.
abstract: Three isolates over 5 years from a patient with persistent relapsing mucosal leishmaniasis due to Leishmania (Viannia) braziliensis and 7 clones from one of these isolates were studied by zymodemes and serodemes analysis. Results showed evidences of clonal phenotypic variation. Eight isoenzymes markers demonstrated clear differences on Cellulose Acetate (CA) and thin starch gel electrophoresis. Also a panel of specific monoclonal antibodies showed such differences. Our observations provide additional evidence that Leishmania (Viannia) braziliensis is composed by subpopulations of parasites with peculiar biochemical and antigenic characteristics.
authors.affiliation: University of Brasília, DF, Brazil.
authors.forename: C A
authors.initials: CA
authors.lastname: Cuba-Cuba
authors.forename: D
authors.initials: D
authors.lastname: Evans
authors.forename: A de C
authors.initials: Ade C
authors.lastname: Rosa
authors.forename: P D
authors.initials: PD
authors.lastname: Marsden
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000940:Antigenic Variation; D015415:Biomarkers / Q000032:analysis; D006224:Cricetinae; D004588:Electrophoresis, Cellulose Acetate; D005455:Fluorescent Antibody Technique; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D007892:Leishmania braziliensis / Q000201:enzymology*; D007897:Leishmaniasis, Mucocutaneous / Q000235:genetics / Q000469:parasitology*
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D007527:Isoenzymes