title: [Evaluation of counterimmunoelectrophoresis with antigens of icterohaemorrhagiae and patoc serovars in the serodiagnosis of human leptospirosis].
abstract: Counterimmunoelectrophoresis (CIE) was applied on paired sera from 135 patients with leptospirosis and on 69 sera from a control group. The sera from patients were subdivided in 4 groups according to the results obtained by the Microscopic Agglutination Test (MAT). The first samples sera from 58 patients were non reagent by MAT. Six monthly samples of sera were taken from 7 patients to follow-up and to determine the level of agglutinin and precipitin antibodies present using MAT and CIE. Serovars icterohaemorrhagiae and patoc were used as antigens. Three types of antigens were compared, 1) Triton-X-100 extracted; 2) heat extracted and 3) a pool of them. The CIE using icterohaemorrhagiae derived antigens types agreed with MAT in 92.64, 92.64 and 94.11% of the leptospirosis sera. The patoc antigens types reacted with the control group in 7.24, 86.95 and 84.05% of the samples, and consequently were eliminated from the present study. The icterohaemorrhagiae CIE reaction become positive earlier than MAT negative sera, and reverted to negative earlier in the follow-up samples from the patients. The CIE was sensitive and specific, gave rapid results and was easy to perform.
authors.affiliation: Instituto de Ciêncías Biomédicas da USP, Depto. de Microbiología, Brasil.
authors.forename: P H
authors.initials: PH
authors.lastname: Yasuda
authors.forename: E E
authors.initials: EE
authors.lastname: Sakata
authors.forename: M A
authors.initials: MA
authors.lastname: Shikanai-Yasuda
authors.forename: S de A
authors.initials: Sde A
authors.lastname: Vasconcelos
authors.forename: E C
authors.initials: EC
authors.lastname: Romero
authors.forename: M V
authors.initials: MV
authors.lastname: da Silva
authors.forename: S
authors.initials: S
authors.lastname: Carrasco
mesh_terms: D000372:Agglutination Tests; D000942:Antigens, Bacterial / Q000097:blood*; D003378:Counterimmunoelectrophoresis; D005188:False Negative Reactions; D005189:False Positive Reactions; D006801:Humans; D007919:Leptospira / Q000276:immunology*; D007921:Leptospira interrogans / Q000276:immunology; D007922:Leptospirosis / Q000175:diagnosis*; D011237:Predictive Value of Tests; D013997:Time Factors; D014895:Weil Disease / Q000175:diagnosis
chemical_list: D000942:Antigens, Bacterial
vernacular_title: Avaliação da contraimunoeletroforese com antígenos dos sorovars icterohaemorrhagiae E patoc no diagnóstico sorológico da leptospirose humana.