title: Vaccination against progressive atrophic rhinitis with a recombinant Pasteurella multocida toxin derivative.
abstract: Vaccination against progressive atrophic rhinitis using a purified recombinant derivative of the Pasteurella multocida toxin (PMT), was carried out. Ten pregnant gilts were vaccinated twice with the nontoxic derivative (dO) which apart from a lack of 121 amino acids had an amino acid sequence identical to PMT, while seven gilts were vaccinated with a purified, formaldehyde treated, native PMT and ten gilts served as non-vaccinated controls. The resulting piglets were inoculated intranasally with Bordetella bronchiseptica and toxigenic P. multocida. Among piglets from the nonvaccinated gilts all except one developed clinical atrophic rhinitis and 90% developed severe turbinate atrophy while only a few pigs in the vaccinated groups developed clinical or pathological signs of disease. Gilt colostra from the two vaccinated groups had similar mean anti-PMT titers and the mean titers in the offspring's sera from these groups were nearly identical throughout the study. No pigs born from unvaccinated gilts were seropositive until 8 wk of age (7 wk post-challenge) but 23% became seropositive at slaughter. The infection rate with toxigenic P. multocida in piglets and the total number of P. multocida colonies cultured from nasal swabs were significantly reduced at 5 wk and 8 wk of age in the vaccinated groups, when compared to controls. There was a significantly improved weight gain (greater than 9%) from birth to slaughter in offspring from vaccinated gilts. No significant differences in feed conversion rate or % lean meat were observed among the groups. The study showed the excellent immunoprotective properties of the nontoxic derivative of the PMT molecule.
authors.affiliation: National Veterinary Laboratory, Animal Biotechnology Research Centre, Copenhagen, Denmark.
authors.forename: J P
authors.initials: JP
authors.lastname: Nielsen
authors.forename: N T
authors.initials: NT
authors.lastname: Foged
authors.forename: V
authors.initials: V
authors.lastname: Sørensen
authors.forename: K
authors.initials: K
authors.lastname: Barfod
authors.forename: A
authors.initials: A
authors.lastname: Bording
authors.forename: S K
authors.initials: SK
authors.lastname: Petersen
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001427:Bacterial Toxins / Q000737:chemistry / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology; D001885:Bordetella Infections / Q000517:prevention & control / Q000662:veterinary; D004435:Eating; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008970:Molecular Weight; D009297:Nasal Mucosa / Q000382:microbiology; D010325:Pasteurella / Q000276:immunology*; D010326:Pasteurella Infections / Q000517:prevention & control / Q000662:veterinary; D011247:Pregnancy; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D012222:Rhinitis, Atrophic / Q000517:prevention & control / Q000662:veterinary*; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D014420:Turbinates / Q000473:pathology; D014611:Vaccination / Q000662:veterinary; D014614:Vaccines, Synthetic; D015430:Weight Gain
chemical_list: D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic