Enhanced motility in NIH 3T3 fibroblasts that overexpress gelsolin.
Increasing the content of the actin-binding protein gelsolin in cultured mouse fibroblasts by up to 125 percent by gene transfection proportionally enhanced the rate at which the cells migrated through porous filters toward a gradient of serum and closed a wound made on a confluent monolayer of cells in a tissue culture dish. These results provide direct evidence that gelsolin, which promotes both actin assembly and disassembly in vitro, is an important element in fibroblast locomotion and demonstrate that the manipulation of intracellular machinery can increase cell motility.
Cunningham
C C
CC
Hematology-Oncology Unit, Massachusetts General Hospital, Boston 02129.
Stossel
T P
TP
Kwiatkowski
D J
DJ
D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D002633:Chemotaxis; D005347:Fibroblasts / Q000502:physiology; D018260:Gelsolin; D015870:Gene Expression; D006801:Humans; D007700:Kinetics; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics / Q000502:physiology*; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
D002135:Calcium-Binding Proteins; D018260:Gelsolin; D008840:Microfilament Proteins; D012333:RNA, Messenger