[The anti-tumor efficacy of natural killer (NK) cells and interleukin 2 against experimental pulmonary metastasis in rats].
Rat spleen cells with NK activity were enriched by discontinuous percoll density gradient centrifugation. Morphologically, about 50% were large granular lymphocytes (LGL), and the majority of these were OX8+ and Asialo GM1+. Cytotoxicity assays in vitro showed that the activity of enriched NK cells was significantly higher than that of unseparated rat spleen cells. Intravenous administration of enriched NK cells plus intraperitoneal crude IL2 led to the successful regression of pulmonary metastasis Walker 256 carcinosarcoma in rats. The effect was much stronger than that caused by unseparated spleen cells plus IL2. The results indicate that adoptive therapy using NK cells instead of spleen cells, in combination with exogenous IL2, might improve the efficiency of tumor immunotherapy.
Luo
L
L
Institute of Basic Medical Sciences, Beijing.
D000818:Animals; D002279:Carcinoma 256, Walker / Q000473:pathology / Q000556:secondary / Q000628:therapy*; D005260:Female; D016219:Immunotherapy, Adoptive; D007376:Interleukin-2 / Q000627:therapeutic use*; D007694:Killer Cells, Natural / Q000276:immunology*; D008175:Lung Neoplasms / Q000556:secondary / Q000628:therapy*; D008297:Male; D009368:Neoplasm Transplantation; D051381:Rats; D011919:Rats, Inbred Strains
D007376:Interleukin-2