title: Chromosome rearrangement in Leishmania mexicana M379.
abstract: Circular extrachromosomal elements were observed in a variety of Leishmania species. We show here that two lines originating from the same isolate have been found to contain a circular DNA molecule of 26.6 kb and a linear chromosome of about 250 kb, respectively, which share a homology of more than 20 kb. The circular DNA molecule and its related region on the linear chromosome were cloned and their restriction maps compared. This investigation reveals information about chromosome rearrangement in L. mexicana M379. Further examination will enable us to understand the nature of chromosome rearrangement such as circularization or linearization.
authors.affiliation: Instituut voor Moleculaire Biologie, Vrije Universiteit Brussel, Belgium.
authors.forename: J H
authors.initials: JH
authors.lastname: Liu
authors.forename: N
authors.initials: N
authors.lastname: Gajendran
authors.forename: D
authors.initials: D
authors.lastname: Muthui
authors.forename: S
authors.initials: S
authors.lastname: Muyldermans
authors.forename: J C
authors.initials: JC
authors.lastname: Dujardin
authors.forename: S
authors.initials: S
authors.lastname: De Doncker
authors.forename: D
authors.initials: D
authors.lastname: Jacquet
authors.forename: D
authors.initials: D
authors.lastname: Le Ray
authors.forename: F
authors.initials: F
authors.lastname: Mathieu-Daudé
authors.forename: R
authors.initials: R
authors.lastname: Hamers
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004270:DNA, Circular / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D007894:Leishmania mexicana / Q000235:genetics*; D011995:Recombination, Genetic; D015183:Restriction Mapping
chemical_list: D004270:DNA, Circular; D016054:DNA, Protozoan