Primary culture of neonatal rat olfactory neurons.
We have prepared primary cultures of purified neonatal rat olfactory neurons. Dissociated olfactory epithelial cells are maintained in modified Eagle's medium with D-valine, cytosine arabinoside, and NGF. NGF is required for neuronal survival. Immunohistochemical staining is positive for the neuronal markers vimentin, olfactory marker protein, and neuron-specific enolase, but negative for the glial markers, glial fibrillary acidic protein, and S-100 protein. Physiologic concentrations of odorants stimulate cAMP accumulation in the cells. Because of their morphology, biochemical composition, and responsiveness to odorants, these cells should enhance olfactory investigations.
Ronnett
G V
GV
Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Hester
L D
LD
Snyder
S H
SH
D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000187:drug effects / Q000502:physiology; D015415:Biomarkers; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D000242:Cyclic AMP / Q000378:metabolism; D004847:Epithelial Cells; D009414:Nerve Growth Factors / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000032:analysis; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D009812:Odorants; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D051381:Rats; D014420:Turbinates / Q000166:cytology / Q000294:innervation*; D014746:Vimentin / Q000032:analysis
D015415:Biomarkers; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D014746:Vimentin; D000242:Cyclic AMP; D010751:Phosphopyruvate Hydratase