Regulation of interleukin-2 gene enhancer activity by the T cell accessory molecule CD28.
The mechanism by which cell surface molecules regulate T cell production of lymphokines is poorly understood. Production of interleukin-2 (IL-2) can be regulated by signal transduction pathways distinct from those induced by the T cell antigen receptor. Stimulation of CD28, a molecule expressed on most human T cells, induced the formation of a protein complex that bound to a site on the IL-2 gene distinct from previously described binding sites and increased IL-2 enhancer activity fivefold. The CD28-responsive complex bound to the IL-2 gene between -164 and -154 base pairs from the transcription start site. The sequence of this element is similar to regions conserved in the 5' flanking regions of several other lymphokine genes.
Fraser
J D
JD
Howard Hughes Medical Institute, University of California, San Francisco 94143.
Irving
B A
BA
Crabtree
G R
GR
Weiss
A
A
D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology*; D001483:Base Sequence; D018106:CD28 Antigens; D002460:Cell Line; D004252:DNA Mutational Analysis; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000235:genetics*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D012045:Regulatory Sequences, Nucleic Acid; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000502:physiology*; D014158:Transcription, Genetic
D000945:Antigens, Differentiation, T-Lymphocyte; D018106:CD28 Antigens; D007376:Interleukin-2; D009841:Oligonucleotides