CD3+ T-cells in severe combined immunodeficiency (scid) mice. III. Transferred congenic, selfreactive CD4+ T cell clones rescue IgM-producing, scid-derived B cells.
Young severe combined immunodeficiency (scid) mice completely lack immunocompetent lymphocytes. Limiting numbers of purified CD4+ T cells from allotype-congenic BALB/c (Igha) donor mice were transplanted into 3-week-old scid (Ighb) recipient mice. Splenic CD4+ T cells were recovered from transplanted scid mice 10-12 weeks post-transfer and established as T cell lines in culture. These T cell populations proliferated in vitro in response to syngeneic stimulator cells. T cell clones derived in vitro from these T cell lines displayed selfreactive recognition specificity: these CD4+ T cells proliferated in vitro in response to syngeneic/congeneic but not allogeneic stimulator cells. Cloned selfreactive T cells retransplanted into young scid recipients were engrafted into spleens of secondary recipients, did not induce autoimmune disease but stimulated development of scid-derived (Ighb), IgM-producing B cells (B cell leakiness).
Reimann
J
J
Institute of Microbiology, Ulm University, FRG.
Rudolphi
A
A
Claesson
M H
MH
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D001402:B-Lymphocytes / Q000276:immunology; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D002999:Clone Cells / Q000276:immunology; D005260:Female; D007075:Immunoglobulin M / Q000096:biosynthesis; D007153:Immunologic Deficiency Syndromes / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D011948:Receptors, Antigen, T-Cell; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D007075:Immunoglobulin M; D011948:Receptors, Antigen, T-Cell