title: Intracellular pH regulates the production of different oxygen metabolites in neutrophils: effects of organic acids produced by anaerobic bacteria.
abstract: The effects of short chain carboxylic acids (SCCA), namely succinic, butyric, and iso-butyric, on neutrophil metabolic activation were assessed. SCCA induced a significant decrease in O2.- recovery and chemiluminescent response in neutrophils activated with the diacylglycerol analog tetradecanoyl-phorbol-acetate (TPA). SCCA did not alter O2 consumption, H2O2 production, or the calorimetrically determined energy expenditure occurring during the metabolic burst. SCCA also induced a significant acidification of intracellular pH (pHi). These results are consistent with an increased divalent versus univalent O2 reduction performed by the NADPH oxidase at a more acidic intracellular pH.
authors.affiliation: Department of Pharmacology and Cell Biology, Forsyth Research Institute, Boston, Massachusetts 02115.
authors.forename: M
authors.initials: M
authors.lastname: Tonetti
authors.forename: A
authors.initials: A
authors.lastname: Cavallero
authors.forename: G A
authors.initials: GA
authors.lastname: Botta
authors.forename: R
authors.initials: R
authors.lastname: Niederman
authors.forename: C
authors.initials: C
authors.lastname: Eftimiadi
mesh_terms: D001421:Bacteria, Anaerobic / Q000502:physiology*; D001711:Biotransformation; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002151:Calorimetry; D002264:Carboxylic Acids / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D058610:Isobutyrates; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010101:Oxygen Consumption / Q000187:drug effects*; D013386:Succinates / Q000494:pharmacology; D019802:Succinic Acid; D013481:Superoxides / Q000097:blood*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D002087:Butyrates; D002264:Carboxylic Acids; D058610:Isobutyrates; D013386:Succinates; D020148:Butyric Acid; D013481:Superoxides; D008165:Luminol; C020380:isobutyric acid; D019802:Succinic Acid; D006861:Hydrogen Peroxide; D013755:Tetradecanoylphorbol Acetate