Characterization of monoclonal antibodies to bovine viral diarrhoea virus: evidence of a neutralizing activity against gp48 in the presence of goat anti-mouse immunoglobulin serum.
Twenty-one monoclonal antibodies (MAbs) directed against the NY-1 and the Osloss-c strains of bovine viral diarrhoea virus were produced and characterized by indirect immunofluorescence assay, radioimmunoprecipitation and neutralization tests. Fourteen MAbs directed against the NY-1 strain recognized the gp48 and showed a weak neutralizing activity in the presence of goat anti-mouse immunoglobulin serum.
Boulanger
D
D
Department of Virology-Immunology, Faculty of Veterinary Medicine, University of Liège, Belgium.
Waxweiler
S
S
Karelle
L
L
Loncar
M
M
Mignon
B
B
Dubuisson
J
J
Thiry
E
E
Pastoret
P P
PP
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006041:Goats; D007136:Immunoglobulins / Q000276:immunology*; D051379:Mice; D009500:Neutralization Tests; D015531:Radioimmunoprecipitation Assay; D014759:Viral Envelope Proteins / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007136:Immunoglobulins; D014759:Viral Envelope Proteins