Effect of carbodiimides on the activity of Mg(2+)-ATPase of slow-twitch muscle microsomal membranes.
1. The hydrophobic N,N'-dicyclohexylcarbodiimide (DCCD) inhibits the activity of Mg(2+)-ATPase of slow-twitch muscle microsomal fraction. 2. The inhibition is dependent on time and concentration, with half-maximal inhibition occurring at 0.4 mM concentration of carbodiimide after a 0.5 hr incubation at room temperature. 3. ATP does not protect against the inhibition. 4. Two water-soluble carbodiimides, 1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide (CMCD) and 1-ethyl-3(3-dimethylaminopropyl)-carbodiimide (EDCD), are not inhibitory. 5. Inhibition of Mg(2+)-ATPase activity by DCCD is accompanied by covalent incorporation of the radioactive agent into the partially purified enzyme preparation.
Leśniak
W
W
Department of Muscle Biochemistry, Nencki Institute of Experimental Biology, Warszawa, Poland.
D000818:Animals; D003028:CME-Carbodiimide / Q000031:analogs & derivatives*; D017301:Ca(2+) Mg(2+)-ATPase / Q000187:drug effects / Q000378:metabolism*; D002234:Carbodiimides / Q000494:pharmacology*; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000494:pharmacology; D007425:Intracellular Membranes / Q000201:enzymology*; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology*; D011817:Rabbits
D002234:Carbodiimides; C060331:1-cyclohexyl-3-(2-(4-morpholinyl)ethyl)carbodiimide; D003028:CME-Carbodiimide; D004024:Dicyclohexylcarbodiimide; D017301:Ca(2+) Mg(2+)-ATPase; D005022:Ethyldimethylaminopropyl Carbodiimide