title: Characterization of products of TY1-mediated reverse transcription in Saccharomyces cerevisiae.
abstract: Transposition of the yeast transposable element, Ty, has been shown to require a reverse transcription process. By analysing the extrachromosomal Ty-specific nucleic acid molecules associated with overproduced Ty virus-like particles (Ty-VLPs), we identified several reverse transcribed cDNA strands. Most of them resemble the characteristic intermediates of the reverse transcription process described for authentic retroviruses: a (-) strong-stop DNA strand covalently bound to an RNA primer, two elongated (-) strands with one or two long terminal repeat (LTR) sequences and a (+) strong-stop DNA. Surprisingly, complete (+) strands and full-length linear duplex Ty DNA could not be detected. The structural features of two additional (+) strands may indicate some differences between the mechanisms of (+) strand synthesis in Ty and other retrotransposons or retroviruses.
authors.affiliation: Institut für Mikrobiologie, Universität Düsseldorf, Federal Republic of Germany.
authors.forename: F
authors.initials: F
authors.lastname: Müller
authors.forename: W
authors.initials: W
authors.lastname: Laufer
authors.forename: U
authors.initials: U
authors.lastname: Pott
authors.forename: M
authors.initials: M
authors.lastname: Ciriacy
mesh_terms: D001483:Base Sequence; D004251:DNA Transposable Elements; D004271:DNA, Fungal / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013728:Terminator Regions, Genetic / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D004251:DNA Transposable Elements; D004271:DNA, Fungal