N-acetylation of aromatic amines: genetic polymorphism in inbred rat strains.
The inheritance of rat liver N-acetyltransferase polymorphism was investigated with reciprocal genetic crosses between slow (NSD/N) and rapid (Peth/N) acetylator strains. Rat liver N-acetyltransferase activity was determined using a spectrophotometric assay which measured the amount of arylamine substrate present after incubation with N-acetyltransferase in vitro. Male N-acetyltransferase activities assayed in liver preparations using p-aminobenzoic acid and p-toluidine as substrates indicate bimodality of the parental strains and unimodality of the F-1 generation; limited data suggest trimodality (not significantly different from a 1:2:1 ratio) of the F-2 generation. Reciprocal crosses of WKY/N, another slow acetylator strain, and the Peth/N strain gave results similar to those of the NSD/N x Peth/N cross. Female N-acetyltransferase activities in all strains studied were lower than male N-acetyltransferase activities, but were similarly distributed in the parental and F-1 generations. The male/female N-acetyltransferase activity ratio was substrate- and genotype-dependent. Results show that regulation of the variation of rat liver N-acetyltransferase activity is consistent with autosomal Mendelian inheritance of two major alleles at a single gene locus.
Juberg
D R
DR
Department of Environmental and Industrial Health, University of Michigan, Ann Arbor 48109.
Bond
J T
JT
Weber
W W
WW
D010129:4-Aminobenzoic Acid / Q000378:metabolism; D000107:Acetylation; D000818:Animals; D001191:Arylamine N-Acetyltransferase / Q000235:genetics* / Q000378:metabolism*; D003433:Crosses, Genetic; D005260:Female; D008099:Liver / Q000201:enzymology*; D008297:Male; D011110:Polymorphism, Genetic; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics*; D011921:Rats, Inbred WKY / Q000235:genetics; D012737:Sex Factors; D014052:Toluidines / Q000378:metabolism
D014052:Toluidines; D001191:Arylamine N-Acetyltransferase; D010129:4-Aminobenzoic Acid