Refrigerated storage of washed red cells.
Red cells washed and stored in a citrate-phosphate-glucose-adenine solution at pH 7.4-7.6 demonstrate excellent maintenance of adenosine triphosphate, elevation of 2,3-diphosphoglycerate well above normal levels for more than 6 weeks, reduced hemolysis and 24-hour in vivo survival comparable to that of cells stored in ADSOL. These results can be attributed in part to a chloride shift in which the washout of intracellular chloride is associated with an influx of OH-, which increases intracellular pH and thereby increases the rate of glycolysis. The phosphate functions primarily as a buffer to maintain both extra- and intracellular pH. Reducing the effective osmolality of the storage solution reduces hemolysis and improves cell morphology.
Meryman
H T
HT
Jerome Holland Laboratory for the Biomedical Sciences, American Red Cross, Rockville, Md.
Hornblower
M
M
Keegan
T
T
Syring
R
R
Heaton
A
A
Mesbah-Karimi
N
N
Bross
J
J
D000255:Adenosine Triphosphate / Q000097:blood; D001793:Blood Preservation / Q000379:methods*; D002712:Chlorides / Q000494:pharmacology; D003080:Cold Temperature; D004789:Enzyme Activation; D004905:Erythrocyte Aging / Q000187:drug effects*; D004911:Erythrocyte Volume / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007038:Hypotonic Solutions; D010710:Phosphates / Q000494:pharmacology; D010732:Phosphofructokinase-1 / Q000187:drug effects; D012709:Serum Albumin / Q000032:analysis; D012996:Solutions
D002712:Chlorides; D007038:Hypotonic Solutions; D010710:Phosphates; D012709:Serum Albumin; D012996:Solutions; D000255:Adenosine Triphosphate; D010732:Phosphofructokinase-1