Expression of the A antigen (gp57-65) of Marek's disease virus by a recombinant baculovirus.
A recombinant baculovirus expressing the A antigen (A Ag) of Marek's disease virus (MDV) was constructed. In Spodoptera fruiperda (Sf) cells infected with the recombinant virus, A Ag expression was localized to the cell surface. Only a small amount of recombinant A Ag was detected in the culture supernatant of infected Sf cells, but authentic A Ag is mainly secreted into the culture supernatant of MDV-infected chicken embryo fibroblasts. Cell surface-associated recombinant A Ag seemed to be slightly larger than authentic A Ag, whereas the secreted recombinant A Ag seemed to be smaller. The recombinant A Ag was shown to be reactive with the sera of MDV-infected chickens by immunodiffusion studies and ELISA. Sera of chickens immunized with recombinant A Ag formed a precipitin line with the culture supernatant of MDV-infected chicken embryo fibroblasts in an immunodiffusion test. These results indicate that the recombinant A Ag expressed by the recombinant baculovirus retains the antigenic and immunogenic properties of the authentic A Ag.
Niikura
M
M
Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Matsuura
Y
Y
Hattori
M
M
Onuma
M
M
Mikami
T
T
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D002642:Chick Embryo; D004279:DNA, Viral / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D008381:Herpesvirus 2, Gallid / Q000276:immunology*; D005779:Immunodiffusion; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D011995:Recombination, Genetic; D014162:Transfection
D000956:Antigens, Viral; D004279:DNA, Viral; C056151:Marek's disease herpesvirus gp57-65