The structure and application of oligodeoxyribonucleotides containing modified, degenerate bases.
Oligodeoxynucleotides containing modified pyrimidine bases which can stably hydrogen-bond to adenine and guanine and also purine bases which pair with thymine and cytosine residues in duplexes have been synthesised, as have oligomers with both such analogues. Structures have been investigated by melting transitions, n.m.r. spectroscopy and crystallography and then interpreted in terms of tautomeric equilibria. Applications to hybridisation probes and primers will be discussed.
Brown
D M
DM
MRC Laboratory of Molecular Biology, Cambridge, UK.
Lin
P K
PK
D001483:Base Sequence; D006860:Hydrogen Bonding; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D013816:Thermodynamics
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides