Protein-protein interactions in lymphocytic choriomeningitis virus.
The structural organization of the lymphocytic choriomeningitis virus (LCMV) particle has been examined by Triton X-114 phase separation and nearest neighbor analyses in order to define protein-protein interactions in the virion. Extraction with Triton X-114 established that the 44-kDa membrane glycoprotein, GP-1, is a peripheral protein and that the 35-kDa glycoprotein, GP-2, is an integral membrane protein. Membrane permeable and membrane impermeable crosslinking reagents were used to establish the structural organization of the virion. Results obtained with both types of crosslinking reagents demonstrated that both GP-1 and GP-2 were assembled as native homotetramers. No covalent or disulfide linkages were found between GP-1 and GP-2, nor were these glycoproteins crosslinked. Protein complexes composed of GP-2 and NP were observed after treatment with a membrane permeable crosslinker (DMS) but not after treatment with the membrane impermeable crosslinker (DTSSP), localizing the site of the GP-2:nucleocapsid protein (NP) interaction to the interior of the virion. The interaction of GP-2 with NP may be important in directing the maturation and budding of LCM virions.
Burns
J W
JW
Department of Neuropharmacology, Scripps Clinic and Research Foundation, La Jolla, California 92037.
Buchmeier
M J
MJ
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003432:Cross-Linking Reagents; D003902:Detergents; D015151:Immunoblotting; D008217:Lymphocytic choriomeningitis virus / Q000378:metabolism* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols; D011485:Protein Binding; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000378:metabolism*; D014771:Virion / Q000378:metabolism / Q000648:ultrastructure
D003432:Cross-Linking Reagents; D003902:Detergents; D014759:Viral Envelope Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40