title: [Comparison of RAST with an immunoenzyme method in simultaneous determination of IgE specific for numerous air allergens].
abstract: Recently, a new immunoenzymatic test (Allergy Screen Profile, ASP) has been proposed for the simultaneous determination of IgE specific for five inhaled allergens (Dermatophagoides, animal dander, trees, grass pollen and Parietaria). For evaluation of this test we compared the results obtained in twenty-seven patients suffering from respiratory allergy with RAST with those obtained using the new test. In 84.3% of cases there was positive concordance between the two tests. The specificity of ASP ranged from 71.4% to 100% and the sensitivity from 76.9% to 95.2% for the different allergens examined. A good correlation between ASP and RAST was particularly evident for Dermatophagoides, animal dander and trees. Therefore, ASP appears to be a suitable test for the simultaneous determination of IgE specific for different allergens.
authors.affiliation: Istituto di Patologia Medica, Università degli Studi di Verona.
authors.forename: M L
authors.initials: ML
authors.lastname: Pacor
authors.forename: G
authors.initials: G
authors.lastname: Marchi
authors.forename: P
authors.initials: P
authors.lastname: Peroli
authors.forename: F
authors.initials: F
authors.lastname: Nicolis
authors.forename: P
authors.initials: P
authors.lastname: Cortina
authors.forename: C
authors.initials: C
authors.lastname: Lunardi
mesh_terms: D000328:Adult; D000388:Air; D000485:Allergens / Q000276:immunology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000032:analysis*; D008297:Male; D011058:Pollen / Q000276:immunology; D011852:Radioallergosorbent Test; D012044:Regression Analysis
chemical_list: D000485:Allergens; D007073:Immunoglobulin E
vernacular_title: Confronto con il RAST di un metodo immunoenzimatico per la determinazione contemporanea delle IgE specifiche verso numerosi aeroallergeni.