title: Production of antibodies to the constant region of the mouse T-cell-receptor beta-chain.
abstract: To generate antibodies to the constant region of the mouse T-cell-receptor beta chain, the corresponding cDNA clone 86T5 was subcloned into the bacterial expression vector pEX2. The induced hybrid protein consisting of a 30 kDa T-cell-receptor segment and a beta-galactosidase carrier moiety was used for immunization. In Western blots using lysates of a T-cell-receptor-positive T-cell hybrid, the antisera obtained reacted with a 42 kDa protein under reducing and a 85 kDa protein under nonreducing conditions. The antisera did not react in binding assays with intact T lymphocytes, i.e. membrane-associated T-cell-receptor protein.
authors.affiliation: Institut für Immunologie, Universität Heidelberg, FRG.
authors.forename: J
authors.initials: J
authors.lastname: Schenkel
authors.forename: H
authors.initials: H
authors.lastname: Schäfer
authors.forename: S
authors.initials: S
authors.lastname: Koch
authors.forename: R
authors.initials: R
authors.lastname: Burger
mesh_terms: D000818:Animals; D000917:Antibody Formation; D004247:DNA / Q000032:analysis; D051379:Mice; D011817:Rabbits; D051381:Rats; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta; D011993:Recombinant Fusion Proteins / Q000276:immunology*
chemical_list: D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D011993:Recombinant Fusion Proteins; D004247:DNA