Human mitochondrial ATP synthase: cloning cDNA for the nuclear-encoded precursor of coupling factor 6.
Coupling factor 6 (F6) is a component of mitochondrial ATP synthase which is required for the interactions of the catalytic and proton-translocating segments. A human fetal muscle cDNA clone encoding this protein was isolated by screening a lambda gt10 library with oligodeoxyribonucleotide probes. The 497-bp F6 cDNA included a 96-bp segment that delineated a presequence of 32 amino acids (aa) in the precursor protein, and 140 bp of 3'-untranslated sequence. The remainder of the cDNA sequence coded for a mature human F6 protein of 76 aa. The deduced primary aa sequence showed 81% homology to that of bovine F6, differing in 14 aa. Almost all of these aa substitutions were conservative and comparison of the hydropathy profiles revealed a similar pattern.
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Department of Cell Physiology, Boston Biomedical Research Institute, MA 02114.
Ogata
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Sanadi
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D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D006801:Humans; D008928:Mitochondria / Q000201:enzymology*; D025261:Mitochondrial Proton-Translocating ATPases; D008969:Molecular Sequence Data; D010086:Oxidative Phosphorylation Coupling Factors / Q000235:genetics*; D006180:Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism
D010086:Oxidative Phosphorylation Coupling Factors; D004247:DNA; D000251:Adenosine Triphosphatases; C000628217:F(6) ATPase; D025261:Mitochondrial Proton-Translocating ATPases; D006180:Proton-Translocating ATPases