Effect of activators and inhibitors on the activity of mitochondrial DNA polymerase.
At a concentration of 0.5 to 3 mmol/l, ATP stimulates the activity of mitochondrial DNA polymerase of Neurospora crassa under the optimum reaction conditions; at higher concentrations, an inhibitory effect is observed. 4-Chloromercuribenzoate (1 mmol/L), a thiol inhibitor, decreases the enzyme activity two-fold, while N-ethylmalcimide (2 mmol/L) has no effect. Ethidium bromide (up to 10 mumol/L) and heparin (up to 0.4 micrograms/mL) reduce the activity by 60%. ddTTP does not affect the DNA polymerase reaction. The best in vitro template is the activated calf-thymus DNA.
Grones
J
J
Department of Biochemistry, Faculty of Nature Science, Comenius University, Bratislava, Czechoslovakia.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D054306:Dideoxynucleotides; D004789:Enzyme Activation / Q000187:drug effects; D004996:Ethidium / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology*; D009492:Neurospora crassa / Q000187:drug effects / Q000201:enzymology*; D019384:Nucleic Acid Synthesis Inhibitors; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D013942:Thymine Nucleotides / Q000494:pharmacology
D054306:Dideoxynucleotides; D019384:Nucleic Acid Synthesis Inhibitors; D013439:Sulfhydryl Reagents; D013942:Thymine Nucleotides; D000255:Adenosine Triphosphate; D006493:Heparin; D004259:DNA-Directed DNA Polymerase; D004996:Ethidium; C017216:2',3'-dideoxythymidine triphosphate