Metabolism of cannabidiol by the rat.
Metabolites of CBD excreted into the bile and perfusion fluid were examined in a rat liver perfusion preparation. Metabolites were extracted with ethyl acetate and identified by GC/MS as TMS derivatives. Four mono- and five di-hydroxy metabolites were identified with major sites of metabolic attack being at C-7 and C-4". A hydroxy-ketone was detected but not fully identified. All biliary metabolites were conjugated with glucuronic acid. Urinary metabolites were studied in rats with samples taken at times to 25 h after drug administration. Unmetabolized CBD and 13 metabolites were identified by GC/MS. Major metabolites were acids with beta-oxidation being a prominent pathway. The 6- and 7-hydroxy derivatives of 4",5"-bis,nor-CBD-3"-oic acid were the most abundant compounds but substantial concentrations of the di-acids, CBD-5",7-dioic acid and 4",5"-bis,nor-CBD-3",7-dioic acid were present. Concentrations of the more highly oxidized metabolites increased with time.
Samara
E
E
University Department of Pharmacology, Oxford, UK.
Bialer
M
M
Harvey
D J
DJ
D000818:Animals; D001646:Bile / Q000378:metabolism; D001711:Biotransformation; D002185:Cannabidiol / Q000378:metabolism* / Q000493:pharmacokinetics; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D005965:Glucuronates / Q000378:metabolism; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D010477:Perfusion; D051381:Rats
D005965:Glucuronates; D002185:Cannabidiol