Effect of magnesium ions and low pH on interaction of pyrimidine oligonucleotides with dsDNA: affinity modification study.
Pyrimidine oligonucleotides bearing 2-chloroethylamino groups bind to corresponding sequences in dsDNA in highly specific way and efficiently alkylate target guanosine residues in purine DNA strand. At acidic pH in the presence of magnesium ions, the oligonucleotides can form nonperfect complexes with partially complementary nucleotide sequences in which some nucleotide units of the oligonucleotides are looped out. Introduction of guanosine residues in pyrimidine oligonucleotides aimed to tolerating thymidines in the purine DNA strand causes a considerable local distortions of the complex structure.
Brossalina
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Siberian Division, USSR Academy of Medical Sciences, Novosibirsk.
Demchenko
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E
Vlassov
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V
D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D015636:Magnesium Chloride; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D011743:Pyrimidines; D013329:Structure-Activity Relationship
D009838:Oligodeoxyribonucleotides; D011743:Pyrimidines; D015636:Magnesium Chloride; D004247:DNA