CD3G is within 200 kb of the leukemic t(4;11) translocation breakpoint.
The t(4;11)(q21;q23) has been associated with acute lymphocytic leukemia (ALL) especially in infants. The t(4;11) breakpoint on chromosome 11 is cytogenetically indistinguishable from breakpoints for other leukemia-associated translocations affecting 11q23. The molecular basis of the t(4;11) is unknown although a number of genes have been mapped to 11q23. The CD3D, G, and E genes have been positioned proximal to the 11q23 breakpoint of the 4;11 translocation while the THY1 and ETS1 genes have been mapped distal to this breakpoint. We report evidence that CD3G is within 200 kb of the 4;11 breakpoint as observed by pulsed field gel analysis. A rearrangement of the CD3G gene has been observed in a cell line derived from a patient with the t(4;11) translocation and in a hybrid cell line containing the derivative 11q chromosome derived from this cell line, using the restriction enzymes SacII and ClaI. Similar rearrangements using SacII were observed in 2 further patients with ALL and the t(4;11) translocation. No rearrangements in the same DNA were observed using ETS1, THY1, and D11S29 and a range of rare cutter restriction enzymes. CD3G thus provides a tool for the cloning and analysis of the 4;11 translocation, and poses a question of its possible involvement at long range with this translocation.
Das
S
S
ICRF Medical Oncology Unit, St. Bartholomew's Hospital, London, U.K.
Cotter
F E
FE
Gibbons
B
B
Dhut
S
S
Young
B D
BD
D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics*; D017252:CD3 Complex; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000648:ultrastructure*; D002894:Chromosomes, Human, Pair 4 / Q000648:ultrastructure*; D004273:DNA, Neoplasm / Q000032:analysis; D005819:Genetic Markers; D006801:Humans; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D015183:Restriction Mapping; D014178:Translocation, Genetic
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D004273:DNA, Neoplasm; D005819:Genetic Markers; D011948:Receptors, Antigen, T-Cell