title: RNase E cleavage in the atpE leader region of atpE/interferon-beta hybrid transcripts in Escherichia coli causes enhanced rates of mRNA decay.
abstract: Chimeric transcripts containing the ribosome binding site of the Escherichia coli atpE gene and variants of the human structural interferon-beta gene are subject to RNase E processing in the 5'-untranslated atpE part of the transcripts. The absence of processing at two sites in the atpE leader-sequence caused by the RNase E deficiency in E. coli host N3431 leads to a considerable stabilization of the mRNA moiety. RNase E has originally been described as a processing enzyme for non-mRNAs such as precursor 5 S rRNA and RNA1, but cleavage mRNA substrates have also been reported. RNase E processing of the atpE gene leader sequence-containing transcripts leads to an increased rate of mRNA breakdown. The two RNase E-dependent processing sites in the atpE part of the mRNA transcripts exhibit some similarity to the other known RNase E processing sites. The influence of RNase E cleavage upon post-transcriptional regulation such as RNA stability and the efficiency of translational initiation is discussed.
authors.affiliation: Gesellschaft für Biotechnologische Forschung, Abt. Genetik, Braunschweig, Federal Republic of Germany.
authors.forename: G
authors.initials: G
authors.lastname: Gross
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D004722:Endoribonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005814:Genes, Viral; D006868:Hydrolysis; D007370:Interferon Type I / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D013379:Substrate Specificity; D014158:Transcription, Genetic
chemical_list: D007370:Interferon Type I; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D004722:Endoribonucleases; C022537:ribonuclease E