title: Transposition of Tn5096 and other IS493 derivatives in Streptomyces griseofuscus.
abstract: Tn5096 was constructed by inserting an apramycin resistance gene, aac(3)IV, into IS493 from Streptomyces lividans. By using conventional and pulsed-field gel electrophoresis, Tn5096 and related transposons were shown to insert into many different locations in the Streptomyces griseofuscus chromosome and in two linear plasmids. On insertion into the target site CANTg, 3 bp appeared to be duplicated. Independent transpositions were obtained by delivery of the transposon from a temperature-sensitive plasmid. The frequency of auxotrophy among cultures containing transpositions was about 0.2%.
authors.affiliation: Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285.
authors.forename: P J
authors.initials: PJ
authors.lastname: Solenberg
authors.forename: R H
authors.initials: RH
authors.lastname: Baltz
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial / Q000235:genetics; D018942:Macrolides; D008969:Molecular Sequence Data; D009328:Nebramycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D010957:Plasmids; D015183:Restriction Mapping; D013171:Spores, Bacterial / Q000502:physiology; D013302:Streptomyces / Q000235:genetics* / Q000502:physiology; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D018942:Macrolides; D009328:Nebramycin; C008250:lankacidins; C011666:apramycin