Three MDBP sites in the immediate-early enhancer-promoter region of human cytomegalovirus.
MDBP, a mammalian sequence-specific DNA-binding protein, was found to recognize two sites in the major immediate-early (IE) enhancer of human cytomegalovirus. The recognition sequence for MDBP at each of these sites was localized to 14 bp by studying the effects of limited G methylation, depurination, depyrimidination, or deoxyribose modification on the ability of these sites to bind to MDBP. In addition to the two high-affinity MDBP sites in the enhancer, one low-affinity MDBP site was detected 5 bp after the transcription initiating residue of this IE transcription unit. The possible biological significance of the two enhancer MDBP sites and the downstream MDBP site is discussed.
Zhang
X Y
XY
Department of Biochemistry, Tulane Medical School, New Orleans, Louisiana 70112.
Inamdar
N M
NM
Supakar
P C
PC
Wu
K
K
Ehrlich
K C
KC
Ehrlich
M
M
D001483:Base Sequence; D001665:Binding Sites; D003587:Cytomegalovirus / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D015967:Gene Expression Regulation, Viral; D006801:Humans; D066298:In Vitro Techniques; D008745:Methylation; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D013329:Structure-Activity Relationship; D014157:Transcription Factors
D004279:DNA, Viral; D004268:DNA-Binding Proteins; C066141:MBD1 protein, human; D014157:Transcription Factors