[Obtaining the allergens from yeast-like fungi of the genus Candida, producers of fodder protein, for hygienic standardization of the strains].
Separated were surface antigens' complex preparations from the native biomass of Candida yeast-like fungi, by using the beta-mercaptoethanol-induced undestructive extraction technique. The preparations were analysed by the highly effective liquid chromatography technique, double immunodiffusion in agar, immunoelectrophoresis, and in experiments on intradermal titration on intact and sensibilized guinea-pigs. It was indicated that the complex preparations of surface antigens extracted from C. maltosa, C. valida and C. scotti were characterized by low reactogenicity and high precision in experimental intracutaneous allergotesting on guinea-pigs. The immunochemical and allergenous activity was higher in preparations with molecular masses of 600 kD. These complex preparations can be used as allergens in the hygienic forming of fodder protein producent strains.
Ermolaev
A V
AV
Gukasian
I A
IA
Parfenova
E V
EV
Spivak
S M
SM
Kravtsov
E G
EG
D000391:Air Microbiology / Q000592:standards*; D000485:Allergens / Q000302:isolation & purification*; D000821:Animal Feed; D000818:Animals; D000946:Antigens, Fungal / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D002175:Candida / Q000276:immunology* / Q000378:metabolism; D004044:Dietary Proteins / Q000378:metabolism; D005656:Fungal Proteins / Q000096:biosynthesis; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000175:diagnosis* / Q000276:immunology; D007428:Intradermal Tests; D012426:Russia
D000485:Allergens; D000946:Antigens, Fungal; D000954:Antigens, Surface; D004044:Dietary Proteins; D005656:Fungal Proteins
Poluchenie allergenov iz drozhzhepodobnykh gribov roda Candida produtsentov kormovogo belka dlia gigienicheskogo normirovaniia shtammov.