Purification and assay of kinesin from sea urchin eggs and early embryos.
This paper describes the procedures used to purify the microtubule motor, kinesin, from mitotic cells, namely sea urchin eggs and cleavage stage embryos, and describes methods for assaying its motor activity.
Buster
D
D
University of California at Davis, Department of Zoology and Graduate Groups in Cell and Developmental Biology, and Biochemistry, CA 95616.
Scholey
J M
JM
D000251:Adenosine Triphosphatases / Q000302:isolation & purification* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002021:Buffers; D002846:Chromatography, Affinity; D016547:Kinesins; D007700:Kinetics; D008870:Microtubules / Q000378:metabolism; D010063:Ovum / Q000737:chemistry; D010784:Photomicrography; D011122:Polyphosphates / Q000378:metabolism; D012617:Sea Urchins / Q000196:embryology; D014743:Videotape Recording
D002021:Buffers; D011122:Polyphosphates; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D016547:Kinesins; C005692:triphosphoric acid