title: A preproinsulin-like pseudogene from Neurospora crassa.
abstract: A segment of DNA was amplified from the Neurospora crassa genome by the polymerase chain reaction using several oligonucleotides coding for highly conserved domains in proinsulin as primers and probe. A genomic clone corresponding to this segment was isolated and the nucleotide sequence was determined. The deduced amino acid sequence of a part of this segment bears remarkable resemblance to preproinsulin, but lacks several requirements for transcription or translation and must therefore be considered to be a pseudogene.
authors.affiliation: Department of Physiology and Biophysics, UMDNJ, Robert Wood Johnson Medical School (at Rutgers), Piscataway 08854-5635.
authors.forename: G
authors.initials: G
authors.lastname: Muthukumar
authors.forename: J
authors.initials: J
authors.lastname: Lenard
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004271:DNA, Fungal; D005800:Genes, Fungal; D006801:Humans; D007328:Insulin; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011384:Proinsulin / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D011544:Pseudogenes; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004271:DNA, Fungal; D007328:Insulin; D011498:Protein Precursors; C013737:preproinsulin; D011384:Proinsulin