title: Isolation and preliminary characterization of Escherichia coli mutants resistant to lethal action of the bacteriophage lambda P gene.
abstract: Both spontaneous and NTG-induced mutants of Escherichia coli 594 insensitive to the lethal action of lambda P gene were isolated and called rpl (resistant to P lethality). These mutants were of two types, showing different phenotypes. On type I rpl mutants, lambda cl- and lambda v1v3 did not plate, while lambda vir, lambda cl- c17, lambda imm434, and lambda imm21 did; plasmid pMR45 carrying the lambda P gene could not complement lambda imm21P- phage in type I mutants. On the other hand, the type II rpl mutants support the growth of all the above phages including lambda cl-. Neither type of rpl mutation affects growth of the bacteria.
authors.affiliation: Department of Biochemistry, Bose Institute, Calcutta, India.
authors.forename: S
authors.initials: S
authors.lastname: Maiti
authors.forename: B
authors.initials: B
authors.lastname: Das
authors.forename: N C
authors.initials: NC
authors.lastname: Mandal
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics* / Q000472:pathogenicity; D004926:Escherichia coli / Q000235:genetics*; D005804:Genes, Lethal; D005814:Genes, Viral; D015678:Viral Structural Proteins / Q000235:genetics*; D014779:Virus Replication
chemical_list: D015678:Viral Structural Proteins