title: Site-specific insertion of three structural gene cassettes in transposon Tn7.
abstract: Transposon Tn7 has been known to carry genes for resistance to trimethoprim and spectinomycin. A poorly expressed streptothricin resistance gene, identical to the sat gene found in transposons Tn1825 and Tn1826, was localized between the two mentioned genes in Tn7. The surroundings of all three resistance genes indicated site-specific insertion of genetic cassettes.
authors.affiliation: Department of Pharmaceutical Microbiology, Uppsala University, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Sundström
authors.forename: P H
authors.initials: PH
authors.lastname: Roy
authors.forename: O
authors.initials: O
authors.lastname: Sköld
mesh_terms: D000595:Amino Acid Sequence; D002470:Cell Survival / Q000187:drug effects; D004254:DNA Nucleotidyltransferases / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics* / Q000502:physiology; D004351:Drug Resistance / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D019426:Integrases; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000235:genetics; D015183:Restriction Mapping; D000198:Spectinomycin / Q000494:pharmacology; D013309:Streptothricins / Q000494:pharmacology; D014295:Trimethoprim / Q000494:pharmacology
chemical_list: D004251:DNA Transposable Elements; D013309:Streptothricins; D000198:Spectinomycin; D014295:Trimethoprim; D004254:DNA Nucleotidyltransferases; D019426:Integrases