Purification of synthetic peptides. Immobilized metal ion affinity chromatography (IMAC).
Immobilized metal ion affinity chromatography (IMAC) is a useful method for purification of synthetic peptides with an N-terminal metal-binding amino acid such as His, Trp, or Cys, especially when such residues are not present in other parts of the molecule. In solid phase peptide synthesis (SPPS), capping with acetic anhydride will, in principle, produce truncated peptides as the only side-products due to incomplete couplings. Consequently, only the desired product will carry the affinity label. Most of the impurities, therefore, can be removed by a single passage through an IMAC column. Some representative examples are presented, where fairly large peptides (30-40 amino acid residues) were efficiently purified by this approach.
Lindeberg
G
G
Department of Immunology, University of Uppsala, Sweden.
Bennich
H
H
Engström
A
A
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000737:chemistry; D023181:Antimicrobial Cationic Peptides; D002480:Cellulase / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D003545:Cysteine / Q000737:chemistry; D006639:Histidine / Q000737:chemistry; D007301:Insect Hormones / Q000737:chemistry; D007475:Ion Exchange Resins / Q000737:chemistry; D008670:Metals / Q000737:chemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification*; D011961:Receptors, Fc / Q000737:chemistry; D017455:Receptors, IgE; D012264:Ribonucleotide Reductases / Q000737:chemistry; D014364:Tryptophan / Q000737:chemistry
D000596:Amino Acids; D000944:Antigens, Differentiation, B-Lymphocyte; D023181:Antimicrobial Cationic Peptides; D002614:Chelating Agents; D007301:Insect Hormones; D007475:Ion Exchange Resins; D008670:Metals; D010455:Peptides; D011961:Receptors, Fc; D017455:Receptors, IgE; D006639:Histidine; C031161:cecropin A; D014364:Tryptophan; D012264:Ribonucleotide Reductases; D002480:Cellulase; D003545:Cysteine