Effects of PGE1 on the development of diabetes and surface markers of lymphocytes in BB/W/Tky rats.
To study the effect of an oral prostaglandin E1 (PGE1) derivative and/or lipo PGE1 on the onset of diabetes, these agents were administered to Bio-Breeding/Worcester/Tokyo (BB/W/Tky) rats from 4 to 13 weeks of age. Lymphocyte subsets in peripheral blood from normal Wistar and BB/W/Tky rats were measured before and for one week after administration of PGE1 using laser flow cytometry. Results showed that 14/16 (87.5%) of the BB/W/Tky rats administered PGE1 developed diabetes vs. 6/12 (50%) in the control BB/W/Tky rats. In normal Wistar rats, PGE1 significantly increased the W3/25+OX39+ and OX8+OX39+ cells. In BB/W/Tky rats, PGE1 significantly increased the OX19+OX6+, OX19+OX39+, W3/25+OX6+, W3/25+OX39+, OX8+, OX8+OX6+, OX8+OX39+ and OX39+ cells, while decreasing the OX6+ cells. We conclude that the administration of PGE1 to BB/W/Tky rats accelerates the development of spontaneous diabetes and alters the surface markers of lymphocytes.
Suzuki
M
M
Fourth Department of Internal Medicine, Saitama Medical School, Japan.
Negishi
K
K
Itabashi
A
A
Katayama
S
S
Ishii
J
J
Komeda
K
K
Kawazu
S
S
D000375:Aging / Q000502:physiology; D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000276:immunology*; D007958:Leukocyte Count / Q000187:drug effects; D016131:Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D051381:Rats; D011913:Rats, Inbred BB; D017208:Rats, Wistar
D000954:Antigens, Surface; D000527:Alprostadil