Chemically induced viral resistance in single preimplantation bovine embryos.
Viral mRNA encoding vesicular stomatitis virus glycoprotein sequences was detected and quantitated using a DNA-hybridization dot-blot technique. This assay was employed to determine if the synthetic double-stranded polynucleotide complex of polyriboinosinic-polyribocytidylic acid would elicit viral resistance in vitro in single day 9 bovine embryos. The levels of viral mRNA were assayed in 4 groups of bovine embryos: unexposed, virus-exposed, polynucleotide-treated, and virus plus polynucleotide treated. Reduced quantities of viral mRNA in single polynucleotide treated embryos demonstrated that resistance to viral infection was induced in day 9 bovine embryos.
Thomson
M S
MS
Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849.
Bird
R C
RC
Stringfellow
D A
DA
Rossi
C R
CR
Lauerman
L H
LH
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects*; D002417:Cattle; D002478:Cells, Cultured; D003001:Cloning, Molecular; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011070:Poly I-C / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000235:genetics*
D012333:RNA, Messenger; D011070:Poly I-C