title: Establishment of immortalized cell lines from mouse peritoneal macrophages following transformation with SV40 early region DNA deleted at the origin of replication.
abstract: Two murine peritoneal macrophage cell lines have been isolated by transforming primary cells with simian virus 40 (SV40) origin-deleted DNA. These lines have been maintained in continuous culture for over 8 months and have been shown to express macrophage-specific properties throughout this time. The cell lines are F4/80 positive; express Fc receptors; will phagocytose immunoglobulin-coated red cells and latex beads; stain with neutral red; and have non-specific esterase and plasminogen activator activities. Lysozyme, collagenase, prostaglandin E2, acid phosphatase and 5'-nucleotidase activities have also been detected and quantified.
authors.affiliation: Department of Bioscience and Biotechnology, Unviersity of Strathclyde, Todd Centre, Glasgow, U.K.
authors.forename: U
authors.initials: U
authors.lastname: Kreuzburg-Duffy
authors.forename: C
authors.initials: C
authors.lastname: Macdonald
mesh_terms: D000818:Animals; D002460:Cell Line / Q000276:immunology; D002472:Cell Transformation, Viral; D002872:Chromosome Deletion; D008264:Macrophages / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010529:Peritoneal Cavity / Q000166:cytology; D011961:Receptors, Fc / Q000032:analysis; D013539:Simian virus 40
chemical_list: D011961:Receptors, Fc