Molecular cloning of human plasma glutathione peroxidase gene and its expression in the kidney.
A genomic clone containing the human plasma glutathione peroxidase (GSH-Px) gene has been isolated using a rat plasma GSH-Px cDNA as a probe. The partial nucleotide sequence of the clone completely matched the sequence of the human plasma GSH-Px cDNA. The results of Southern blot hybridization indicate that the human plasma GSH-Px gene consists of at least 4 exons and 3 introns, and spans about 12 kb. RNA blot analysis demonstrated that the human plasma GSH-Px gene is expressed in the kidney.
Yoshimura
S
S
Department of Cell Biology, Tokai University School of Medicine, Kanagawa, Japan.
Suemizu
H
H
Taniguchi
Y
Y
Watanabe
K
K
Nomoto
Y
Y
Katsuoka
Y
Y
Arimori
S
S
Moriuchi
T
T
D000818:Animals; D003001:Cloning, Molecular; D005979:Glutathione Peroxidase / Q000096:biosynthesis / Q000097:blood / Q000235:genetics*; D006801:Humans; D007668:Kidney / Q000201:enzymology*; D009928:Organ Specificity / Q000235:genetics; D051381:Rats; D015183:Restriction Mapping
D005979:Glutathione Peroxidase