Sequence analysis of the 3'-end of feline calicivirus genome.
The nucleotide sequence of the 3'-end of the Japanese F4 strain of feline calicivirus (FCV) RNA was determined from a cloned cDNA of 3.5 kbp. We found three open reading frames (ORFs). The largest ORF encoded a 668-amino acid protein of 73,588 Da, which was presumably the capsid precursor protein of FCV and had significant amino acid sequence homology with the VP3 of picornaviruses. A small ORF at the extreme 3'-end was compared with that of the F9 strain of FCV, a vaccine strain originally from the U.S. Highly conserved amino acid sequences were shown, suggesting that this ORF might be functional and encode a putative 106-amino acid protein of 12,153 Da. The other ORF in the 5'-flanking region of the cDNA had consensus amino acid sequences conserved among the RNA-dependent RNA polymerases.
Tohya
Y
Y
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Taniguchi
Y
Y
Takahashi
E
E
Utagawa
E
E
Takeda
N
N
Miyamura
K
K
Yamazaki
S
S
Mikami
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002139:Caliciviridae / Q000235:genetics*; D002415:Cats; D003001:Cloning, Molecular; D004279:DNA, Viral; D005814:Genes, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012367:RNA, Viral; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral; D012367:RNA, Viral