title: The antibody response in breast-fed and non-breast-fed infants after artificial colonization of the intestine with Escherichia coli O83.
abstract: The local and systemic antibody response after oral administration of a nonenteropathogenic type 1 fimbriated Escherichia coli O83 strain was followed in nine breast-fed and eight formula-fed infants during their first 15 wk of life. Five breast-fed and six formula-fed infants were followed as controls. E. coli O83 was detected in the stools of colonized infants from d 2 after colonization and persisted in the intestine for up to 26 wk. The percentage of children successfully colonized with E. coli O83 was higher among breast-fed than among formula-fed colonized infants. Also, the O83 bacteria isolated from the breast-fed children had a higher capacity to attach to colonic epithelial cells of the HT-29 cell line than those isolated from bottle-fed infants. E. coli O83 IgA and IgM antibodies estimated by ELISA were significantly elevated in the saliva of colonized as compared with control infants 2-7 wk after colonization. IgA antibodies against O83 were also higher in the stool of colonized formula-fed infants than in formula-fed controls. The results suggest that the mucosal immune system of the newborn infant can be triggered early to produce specific antibodies against bacteria colonizing the intestine.
authors.affiliation: Institute for Care of Mother and Child, Czechoslovak Academy of Science, Prague.
authors.forename: R
authors.initials: R
authors.lastname: Lodinová-Zádníková
authors.forename: M
authors.initials: M
authors.lastname: Slavíková
authors.forename: H
authors.initials: H
authors.lastname: Tlaskalová-Hogenová
authors.forename: I
authors.initials: I
authors.lastname: Adlerberth
authors.forename: L A
authors.initials: LA
authors.lastname: Hanson
authors.forename: A
authors.initials: A
authors.lastname: Wold
authors.forename: B
authors.initials: B
authors.lastname: Carlsson
authors.forename: C
authors.initials: C
authors.lastname: Svanborg
authors.forename: L
authors.initials: L
authors.lastname: Mellander
mesh_terms: D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D001942:Breast Feeding; D004926:Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology / Q000517:prevention & control; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis; D007223:Infant; D007225:Infant Food; D007231:Infant, Newborn; D007422:Intestines / Q000276:immunology / Q000382:microbiology; D008297:Male
chemical_list: D000907:Antibodies, Bacterial; D007070:Immunoglobulin A