Determination of cytoplasmic 5'-nucleotidase which preferentially hydrolyses 6-hydroxypurine nucleotides in pig, rat and human tissues by immunotitration.
1. Activity of a 5'-nucleotidase which preferentially hydrolyses IMP and GMP was determined by immunotitration in various mammalian tissues. 2. Activity per g of rat tissue was high in testis and spleen and low in skeletal muscle. 3. In human cells, the activity was high in fibroblasts and low in erythrocytes.
Itoh
R
R
National Institute of Health and Nutrition, Tokyo, Japan.
Yamada
K
K
D015720:5'-Nucleotidase / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D006157:Guanosine Monophosphate / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D007291:Inosine Monophosphate / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D013552:Swine; D014018:Tissue Distribution
D007291:Inosine Monophosphate; D006157:Guanosine Monophosphate; D015720:5'-Nucleotidase