Purification of megakaryocyte differentiation activity from a human fibrous histiocytoma cell line: N-terminal sequence homology with activin A.
We purified a protein possessing a potent ability to induce the differentiation of a murine megakaryoblastic cell line, L8057, from the supernatant of a human fibrous histiocytoma cell line, KHM-5M. The protein, a homodimer of a molecular weight of 25-kDa, has an N-terminal sequence identical with that of the beta A-chain of inhibin, indicating that it is identical to or highly homologous with activin A. Thus, it is speculated that activin A or its homologue is involved in megakaryocytic differentiation.
Fujimoto
K
K
Second Department of Internal Medicine, Kumamoto University Medical School, Japan.
Kawakita
M
M
Kato
K
K
Yonemura
Y
Y
Masuda
T
T
Matsuzaki
H
H
Hirose
J
J
Isaji
M
M
Sasaki
H
H
Inoue
T
T
D028341:Activins; D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D018219:Histiocytoma, Benign Fibrous; D006801:Humans; D007265:Inhibins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D046911:Macromolecular Substances; D008533:Megakaryocytes / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D046911:Macromolecular Substances; D028341:Activins; D007265:Inhibins