High-performance liquid chromatographic method for the simultaneous measurement of floxuridine and fluorouracil in human body fluids.
A method for the simultaneous measurement of floxuridine (5-fluorodeoxyuridine) and fluorouracil in human plasma and peritoneal fluid has been developed. This method utilizes high-performance liquid chromatographic analysis with a Zorbax RX column (25 cm x 4.6 mm I.D.) plus a guard cartridge of the same material. The sensitivity limits for this assay are 0.25 mumol/l in a 20-microliters sample. The detection limit at a signal-to-noise ratio of 3 is 2.5 nmol/l. This procedure has been used to quantitatively measure concentration versus time profiles of floxuridine and fluorouracil in plasma and peritoneal fluid of human patients after receiving intraperitoneal administration of floxuridine.
Smith-Rogers
J A
JA
Department of Pharmacy, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Tong
W P
WP
Duafala
M E
ME
Markman
M
M
Bertino
J R
JR
D001202:Ascitic Fluid / Q000737:chemistry*; D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D005260:Female; D005467:Floxuridine / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D005472:Fluorouracil / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D006801:Humans; D008297:Male; D008875:Middle Aged
D005467:Floxuridine; D005472:Fluorouracil