Numerous transitions in human parainfluenza virus 3 RNA recovered from persistently infected cells.
The nucleotide sequences at the 3'-termini of human parainfluenza virus 3 (HPIV3) genomic RNAs recovered from two lines of persistently infected LLC-MK2 cells were determined following PCR amplification. After 29 months of persistence the 3'-end of the HPIV3 genome was found to be highly mutated. Interestingly, the only types of nucleotide changes observed were U to C and A to G transitions. Both U to C and A to G transitions were present on individual RNA molecules. The data indicate that biased hypermutational activity leading to U To C and A to G mutations operates in cultured cells during persistent HPIV3 infections.
Murphy
D G
DG
Department of Microbiology and Immunology, University of Ottawa, Faculty of Medicine, Ontario, Canada.
Dimock
K
K
Kang
C Y
CY
D001483:Base Sequence; D002460:Cell Line; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D018184:Paramyxoviridae Infections / Q000235:genetics; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000737:chemistry*; D018108:Respirovirus / Q000235:genetics*
D012367:RNA, Viral