Expression of the major inner capsid protein of the group B rotavirus ADRV: primary characterization of genome segment 5.
A complete cDNA copy of the fifth RNA segment of the human group B rotavirus, ADRV, has been cloned into plasmid AD512. Gene segment 5 contains 1269 bases and encodes one long open reading frame of 391 amino acids beginning at base 31 and terminating at base 1203. The gene 5 polypeptide, expressed in vitro in a rabbit reticulocyte lysate, comigrates with the 44-kDa major inner capsid protein present on EDTA treated ADRV virions. The gene 5 protein is immunoprecipitable by hyperimmune serum to ADRV, human ADRV convalescent serum and by a group B-specific monoclonal antibody. In addition, this protein shares amino acid identity and similarity with the VP6 proteins from group C and group A rotavirus strains. The ADRV VP6 equivalent protein appears to be more closely related to the group C VP6 than the Group A VP6 polypeptide and a common ancestral rotavirus VP6 precursor protein is suggested. As a result, the fifth RNA segment of ADRV defines the major inner capsid protein, or VP6 equivalent, in the group B rotavirus. Expression of the ADRV VP6 equivalent protein is potentially useful for evaluating the prevalence of serum antibodies to group B rotavirus in human and animal populations as well as for generating antibodies for the direct detection of group B rotavirus antigen.
Chen
G M
GM
Department of Microbiology and Immunology, Stanford University, California 94305.
Werner-Eckert
R
R
Tao
H
H
Mackow
E R
ER
D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D002213:Capsid / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*; D014771:Virion / Q000032:analysis
D012367:RNA, Viral; D015678:Viral Structural Proteins; D004247:DNA