title: Topical chemotherapy in human periodontitis using a new controlled-release insert containing ofloxacin. I. Microbiological observation.
abstract: The recognition that destructive periodontal diseases may be caused by specific microorganisms in periodontal pockets has led to an increased interest in and usage of antimicrobial agents in periodontal therapy. Recently, a new controlled-release insert containing ofloxacin, a synthetic antibiotic, has been developed. In this study, the controlled-release insert (PT-01) was microbiologically evaluated in combination with or without subgingival mechanical debridement. PT-01 was applied in the periodontal pockets of 27 patients with chronic periodontitis. Three sites with a deep probing pocket depth (greater than or equal to 5 mm) were randomly selected in different quadrants of each patient, and were assigned into three groups, i.e., PT-01 applied (T), placebo applied (P) and control sites (C). Periodontal treatments consisted of supragingival scaling with oral hygiene instruction for the first 2 weeks followed by root planing and subgingival scaling PT-01 was applied weekly on day 0 to 35, and the subgingival plaque samples from each site were collected on d 0, 14, 21 and 42. The dynamics of the subgingival microflora was investigated by dark field microscopy and by anaerobic and aerobic cultivation. In the supragingival scaling period, significant reduction in percentages of spirochetes and motile rods and significant increase of the percentage of coccoid cells were observed only at T sites. In addition, the total viable counts of bacteria, black-pigmented Bacteroides and Fusobacterium species were significantly reduced at T sites. After mechanical subgingival debridement, significant shifts in the proportion and reduction of the viable counts in the subgingival microflora were found at all sites.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kimura
authors.forename: H
authors.initials: H
authors.lastname: Toda
authors.forename: Y
authors.initials: Y
authors.lastname: Shimabukuro
authors.forename: M
authors.initials: M
authors.lastname: Kitamura
authors.forename: N
authors.initials: N
authors.lastname: Fujimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Miki
authors.forename: H
authors.initials: H
authors.lastname: Okada
mesh_terms: D000287:Administration, Topical; D000328:Adult; D000704:Analysis of Variance; D001419:Bacteria / Q000187:drug effects; D002908:Chronic Disease; D015169:Colony Count, Microbial; D003692:Delayed-Action Preparations; D003773:Dental Plaque / Q000382:microbiology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015242:Ofloxacin / Q000008:administration & dosage* / Q000494:pharmacology; D010518:Periodontitis / Q000188:drug therapy* / Q000382:microbiology
chemical_list: D003692:Delayed-Action Preparations; D015242:Ofloxacin