Inhibition of SV40 gene expression by microinjected small antisense RNA and DNA molecules.
We tested the impact of antisense RNA and DNA molecules on SV40 gene expression by microinjection into TC7 cells. Short antisense stretches, complementary to either hairpin or loop structures on the T antigen mRNA, inhibited T antigen synthesis. In contrast, full-length antisense RNA and DNA molecules did not effect T antigen synthesis.
Graessmann
M
M
Institut für Molekularbiologie und Biochemie der Freien Universität Berlin, FRG.
Michaels
G
G
Berg
B
B
Graessmann
A
A
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis*; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D003198:Computer Simulation; D016373:DNA, Antisense / Q000494:pharmacology*; D004279:DNA, Viral / Q000187:drug effects / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D005814:Genes, Viral; D007700:Kinetics; D008845:Microinjections; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016372:RNA, Antisense / Q000494:pharmacology*; D012367:RNA, Viral / Q000187:drug effects / Q000494:pharmacology; D051381:Rats; D013539:Simian virus 40 / Q000187:drug effects / Q000235:genetics*
D000952:Antigens, Polyomavirus Transforming; D016373:DNA, Antisense; D004279:DNA, Viral; D016372:RNA, Antisense; D012367:RNA, Viral