A cis-acting element responsible for early gene induction by extracellular cAMP in Dictyostelium discoideum.
We have analysed the promoter of the Dictyostelium discoideum alpha-L-fucosidase (ALF) gene, and have identified a 58 bp fragment responsible for transcriptional activation mediated by extracellular cAMP. Replacement of regulatory sequences in the cAMP-independent actin 15 promoter by this fragment confers cAMP regulation to the hybrid promoter. A cAMP analog with high affinity to the cell surface cAMP receptor can induce transcription from the endogenous as well as from the hybrid promoter. Gel-shift experiments show that the 58 bp fragment is a target for nuclear DNA-binding proteins, and that a specific complex is formed in response to cAMP stimulation. The major cAMP-dependent DNA.protein complex is formed with a 22 bp subfragment which we have termed DCRE (Dictyostelium cAMP responsive element).
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Max-Planck-Institut für Biochemie, Abt. Zellbiologie, Martinsried, F.R.G.
Blusch
J
J
Sachse
A
A
Nellen
W
W
D001482:Base Composition; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D000242:Cyclic AMP / Q000502:physiology*; D004023:Dictyostelium / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D015398:Signal Transduction / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D015533:Transcriptional Activation; D005644:alpha-L-Fucosidase / Q000235:genetics
D000242:Cyclic AMP; D015500:Chloramphenicol O-Acetyltransferase; D005644:alpha-L-Fucosidase