Effects of nerve crush and transection on mRNA levels for nerve growth factor receptor in the rat facial motoneurons.
We found that the level of nerve growth factor receptor (NGF-R) mRNA in facial motoneurons was increased after both facial nerve crushing and transection by means of in situ hybridization histochemistry. The increased level of NGF-R mRNA was maintained for at least 8 weeks after facial nerve transection, while facial nerve crushing caused only a transient increase. Thus, expression of NGF-R mRNA paralleled the axonal regeneration process. In addition, the increase of NGF-R mRNA with crushing was more pronounced than with transection from the 3rd to the 14th day after the insult.
Saika
T
T
Department of Otolaryngology, Osaka University Medical School, Japan.
Senba
E
E
Noguchi
K
K
Sato
M
M
Yoshida
S
S
Kubo
T
T
Matsunaga
T
T
Tohyama
M
M
D000818:Animals; D003714:Denervation; D005154:Facial Nerve / Q000378:metabolism / Q000502:physiology; D020220:Facial Nerve Injuries; D005786:Gene Expression Regulation; D008297:Male; D009046:Motor Neurons / Q000378:metabolism*; D009409:Nerve Crush; D009414:Nerve Growth Factors / Q000378:metabolism*; D009416:Nerve Regeneration; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics; D017475:Receptors, Nerve Growth Factor
D009414:Nerve Growth Factors; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor