Heterogeneity of the lymphokine-activated killer cell phenotype.
Lymphokine-activated killer cells (LAK) are functionally defined by their ability to mediate the MHC-unrestricted lysis of a range of tumor targets, while sparing normal cells. They can also lyse TNP-modified normal syngeneic lymphoblasts. We show here that lysis of TNP-modified targets is mediated by CD8+ LAK in a self-MHC-restricted manner, whereas lysis of tumor targets is largely by CD8- LAK and is MHC-unrestricted. LAK generated from the immune-deficient strains Balb/c nude and C.B-17 scid lyse tumor targets as effectively as LAK from normal mice but do not lyse TNP-modified normal targets. Further, lysis of TNP-modified targets, but not tumors, can be inhibited by antibody to the T cell receptor complex. These experiments strongly suggest that recognition of TNP-modified targets is not accomplished by the same mechanism as that of tumors. Rather, they are consistent with recognition of TNP-modified targets by CD8+ LAK cells being mediated via recognition through the T cell receptor.
Chadwick
B S
BS
Ontario Cancer Institute, University of Toronto, Canada.
Miller
R G
RG
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis / Q000502:physiology; D017252:CD3 Complex; D016827:CD8 Antigens; D003602:Cytotoxicity, Immunologic; D006241:Haptens; D007111:Immunity, Cellular; D007153:Immunologic Deficiency Syndromes / Q000276:immunology; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D008819:Mice, Nude / Q000276:immunology; D009374:Neoplasms, Experimental / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D014301:Trinitrobenzenes / Q000276:immunology
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D016827:CD8 Antigens; D006241:Haptens; D011948:Receptors, Antigen, T-Cell; D014301:Trinitrobenzenes