Effect of dibenzo[a,c]cyclooctene lignans isolated from Fructus schizandrae on lipid peroxidation and anti-oxidative enzyme activity.
The effect of nine dibenzo[a,c]cyclooctene lignans isolated from Fructus schizandrae on in vitro and in vivo lipid peroxidation of liver microsomes as well as on anti-oxidative enzyme activities were studied. Seven of the nine lignans (1 mM) were shown to inhibit Vit C/NADPH induced lipid peroxidation (malondialdehyde (MDA) formation) of rat liver microsomes. Of these compounds, schisanhenol (Sal), S(-)schizandrin C (S(-)sin C) and S(-)schizandrin B (S(-)sin B) were shown to be more potent than Vit E at the same concentration. Sal and Sin B were able to inhibit gossypol-induced superoxide anion generation in rat liver microsomes. In addition, oral administration of Sal and Sin B markedly reduced liver MDA formation induced by ethanol, 15 ml/kg in mice, and increased superoxide dismutase and catalase activities in rat liver cytosol. The data of this paper are in favor of the conclusion that some lignans, like Sal, have strong anti-oxidant activity. The mechanisms of anti-oxidant activity of the lignans were discussed.
Lu
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Department of Pharmacology, Chinese Academy of Medical Sciences, Beijing.
Liu
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GT
D000818:Animals; D002374:Catalase / Q000378:metabolism; D034242:Cyclooctanes; D003600:Cytosol / Q000201:enzymology; D000431:Ethanol / Q000494:pharmacology; D006072:Gossypol / Q000494:pharmacology; D017705:Lignans; D008031:Lignin / Q000302:isolation & purification / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000201:enzymology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D009243:NAD / Q000494:pharmacology; D010084:Oxidation-Reduction; D010946:Plants, Medicinal / Q000032:analysis*; D011083:Polycyclic Compounds / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D034242:Cyclooctanes; D017705:Lignans; D011083:Polycyclic Compounds; C015499:schizandrin B; D009243:NAD; D013481:Superoxides; D000431:Ethanol; D008315:Malondialdehyde; C045309:schisanhenol; D008031:Lignin; D002374:Catalase; D013482:Superoxide Dismutase; D006072:Gossypol