title: Molecular cloning of cDNA encoding adipogenesis inhibitory factor and identity with interleukin-11.
abstract: A cDNA encoding a novel adipogenesis inhibitory factor (AGIF) that inhibits the process of adipogenesis in mouse 3T3-L1 preadipocytes was cloned from a cDNA library of the human bone marrow-derived stromal cell line KM-102. The cloned cDNA contains an open reading frame coding for an AGIF precursor of 199 amino acids. Analysis of the sequence of this cDNA revealed identity of this factor with a recently reported novel cytokine, designated interleukin-11 (IL-11). AGIF/IL-11 may play an important role in stromal cell-associated hematopoeisis through its regulatory action on adipocyte differentiation in the bone marrow microenvironment.
authors.affiliation: Bioscience Research Laboratories, Sankyo Co. Ltd., Tokyo, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Kawashima
authors.forename: J
authors.initials: J
authors.lastname: Ohsumi
authors.forename: K
authors.initials: K
authors.lastname: Mita-Honjo
authors.forename: K
authors.initials: K
authors.lastname: Shimoda-Takano
authors.forename: H
authors.initials: H
authors.lastname: Ishikawa
authors.forename: S
authors.initials: S
authors.lastname: Sakakibara
authors.forename: K
authors.initials: K
authors.lastname: Miyadai
authors.forename: Y
authors.initials: Y
authors.lastname: Takiguchi
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D001853:Bone Marrow; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D015723:Gene Library; D006801:Humans; D017370:Interleukin-11; D007378:Interleukins / Q000235:genetics* / Q000494:pharmacology; D008071:Lipoprotein Lipase / Q000037:antagonists & inhibitors; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D014162:Transfection
chemical_list: D017370:Interleukin-11; D007378:Interleukins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D004247:DNA; D008071:Lipoprotein Lipase