Analysis of equine humoral immune responses to the transmembrane envelope glycoprotein (gp45) of equine infectious anemia virus.
Defined segments of the transmembrane envelope glycoprotein (gp45) of equine infectious anemia virus were expressed as TrpLE fusion proteins and examined for their reactivity in Western immunoblots against a diverse panel of equine immune sera. The most immunogenic region of gp45 was localized to its amino terminus, positioned between the hydrophobic fusion and the transmembrane domains. A series of overlapping synthetic peptides were used in enzyme-linked immunosorbent assays to define an immunodominant epitope within this region. In contrast, the carboxy-terminal half of gp45 displayed both weak and variable immunoreactivity with equine immune sera.
Chong
Y H
YH
Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pennsylvania 15261.
Ball
J M
JM
Issel
C J
CJ
Montelaro
R C
RC
Rushlow
K E
KE
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D002462:Cell Membrane / Q000648:ultrastructure; D004279:DNA, Viral / Q000235:genetics; D004859:Equine Infectious Anemia / Q000276:immunology; D005814:Genes, Viral; D006736:Horses / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D004860:Infectious Anemia Virus, Equine / Q000235:genetics / Q000276:immunology*; D008961:Models, Structural; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000276:immunology; D015183:Restriction Mapping; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
D004279:DNA, Viral; D007106:Immune Sera; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; C066797:transmembrane envelope glycoprotein gp45, retrovirus