Porphyrin synthesis by human hepatocytes and HepG2 cells--effects of enzyme inducers and delta-aminolevulinic acid.
Experiments were carried out to investigate the possibility of inducing porphyria in human hepatocytes and HepG2 cells in culture. After treatment with hexachlorobenzene, 3-methylcholanthrene, phenobarbital or dimethyl sulfoxide, protoporphyrin was the predominating porphyrin accumulating in presence of delta-aminolevulinic acid. The typical uroporphyrin accumulation, as is seen in hexachlorobenzene-induced porphyria in vivo, was absent. In HepG2 cells, the activities of uroporphyrinogen decarboxylase and porphobilinogen deaminase were not influenced by cytochrome P-450 inducers, hexachlorobenzene or dimethyl sulfoxide during 48 h of culture. Therefore, the use of these cells in the study of porphyria cutanea tarda does not seem promising.
Visser
O
O
Department of Internal Medicine II, Erasmus University Rotterdam, The Netherlands.
van den Berg
J W
JW
Koole-Lesuis
H
H
Voortman
G
G
Wilson
J H
JH
D000622:Aminolevulinic Acid / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004790:Enzyme Induction; D006581:Hexachlorobenzene / Q000494:pharmacology; D006801:Humans; D011163:Hydroxymethylbilane Synthase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008113:Liver Neoplasms; D008748:Methylcholanthrene / Q000494:pharmacology; D010634:Phenobarbital / Q000494:pharmacology; D011166:Porphyrins / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured; D014575:Uroporphyrinogen Decarboxylase / Q000378:metabolism
D011166:Porphyrins; D006581:Hexachlorobenzene; D008748:Methylcholanthrene; D000622:Aminolevulinic Acid; D003577:Cytochrome P-450 Enzyme System; D011163:Hydroxymethylbilane Synthase; D014575:Uroporphyrinogen Decarboxylase; D004121:Dimethyl Sulfoxide; D010634:Phenobarbital