Ligand autoradiographic receptor screening. II. Expression of receptor cDNA in transfected COS cells grown on polyester disks and its recovery.
A receptor autoradiographic method that can detect expressed receptor cDNA in 0.002% of plasmid clones and can allow cDNA recoveries and enrichments up to 300-fold in one cycle of screening is described.
Schaeffer
J C
JC
Laboratory of Molecular Neurobiology, ARC/NIDA, Baltimore, MD 21224.
Lin
C L
CL
Kitayama
S
S
Uhl
G R
GR
D000818:Animals; D001345:Autoradiography; D002461:Cell Line, Transformed; D004247:DNA / Q000096:biosynthesis* / Q000302:isolation & purification; D008024:Ligands; D011091:Polyesters; D011956:Receptors, Cell Surface / Q000235:genetics*; D015203:Reproducibility of Results; D014162:Transfection / Q000235:genetics
D008024:Ligands; D011091:Polyesters; D011956:Receptors, Cell Surface; D004247:DNA