Identification of F0 subunits in the rat liver mitochondrial F0F1-ATP synthase.
In order to identify the subunits constituting the rat liver F0F1-ATP synthase, the complex prepared by selective extraction from the mitochondrial membranes with a detergent followed by purification on a sucrose gradient has been compared to that obtained by immunoprecipitation with an anti-F1 serum. The subunits present in both preparations that are assumed to be authentic components of the complex have been identified. The results show that the total rat liver F0F1-ATP synthase contains at least 13 different proteins, seven of which can be attributed to F0. The following F0 subunits have been identified: the subunit b (migrating as a 24 kDa band in SDS-PAGE), the oligomycin-sensitivity-conferring protein (20 kDa), and F6 (9 kDa) that have N-terminal sequences homologous to the beef-heart ones; the mtDNA encoded subunits 6 (20 kDa) and 8 (less than 7 kDa) that can be synthesized in isolated mitochondria; an additional 20 kDa protein that could be equivalent to the beef heart subunit d.
Cretin
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LBTM-CNRS, Université Cl. Bernard de Lyon I, Villeurbanne, France.
Baggetto
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LG
Denoroy
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L
Godinot
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C
D000251:Adenosine Triphosphatases / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D008930:Mitochondria, Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D051381:Rats
D004272:DNA, Mitochondrial; D000251:Adenosine Triphosphatases