Quantitative assay of terminal deoxynucleotidyl transferase (TdT) activity using monoclonal antibodies.
Terminal deoxynucleotidyl transferase (TdT) is an important marker for the diagnosis and the therapy of leukemia and lymphoma patients. In this study, we developed the quantitative method for the assay of TdT antigen in leukemic cells using a combination of monoclonal and polyclonal antibodies prepared for this enzyme. High correlation was obtained between the values measured by biochemical assay and enzyme-linked immunosorbent assay (ELISA). This method (ELISA), using antibodies to TdT, permits the rapid and quantitative estimation of TdT antigen in leukemic cells.
Sasaki
R
R
Department of Hematology, Jichi Medical School, Tochigi-Ken, Japan.
Yuasa
Y
Y
Bollum
F J
FJ
Minowada
J
J
Miura
Y
Y
Takaku
F
F
D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D004253:DNA Nucleotidylexotransferase / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D066298:In Vitro Techniques; D007938:Leukemia / Q000201:enzymology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D004253:DNA Nucleotidylexotransferase