Engraftment and development of human T and B cells in mice after bone marrow transplantation.
A model for human lymphocyte ontogeny has been developed in a normal mouse. Human bone marrow, depleted of mature T and B lymphocytes, and bone marrow from mice with severe combined immunodeficiency were transplanted into lethally irradiated BALB/c mice. Human B and T cells were first detected 2 to 4 months after transplantation and persisted for at least 6 months. Most human thymocytes (30 to 50 percent of total thymocytes) were CD3+CD4+CD8+. Human immunoglobulin was detected in some chimeras, and a human antibody response to dinitrophenol could be generated after primary and secondary immunization.
Lubin
I
I
Department of Biophysics, Weizmann Institute of Science, Rehovot, Israel.
Faktorowich
Y
Y
Lapidot
T
T
Gan
Y
Y
Eshhar
Z
Z
Gazit
E
E
Levite
M
M
Reisner
Y
Y
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D017252:CD3 Complex; D002454:Cell Differentiation; D002678:Chimera; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006433:Hemocyanins / Q000276:immunology; D006801:Humans; D007114:Immunization; D007136:Immunoglobulins / Q000032:analysis; D007153:Immunologic Deficiency Syndromes; D016130:Immunophenotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D014183:Transplantation, Heterologous
C042158:2,4-dinitrophenyl keyhole limpet hemocyanin; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D007136:Immunoglobulins; D011948:Receptors, Antigen, T-Cell; D006433:Hemocyanins