Altered expression of HLA antigens and CD16 Fc receptors on leukocytes of alcoholic subjects and uremic patients.
The possible influences of ethanol and its metabolic product acetate on the surface expression of HLA class I and class II antigens and CD16 Fc receptors were examined. Fluorescent-labeled monoclonal antibodies and flow cytometry were used to measure these antigens on leukocytes from reference controls, subjects admitted for alcohol detoxification, uremic patients undergoing hemodialysis using Cu-prophan dialyzers and fluids containing 4 to 37 mM acetate, and uremic patients that were not hemodialyzed. In comparison to the controls, the mean intensity of staining for class I antigens was not changed significantly on lymphocytes or monocytes from alcoholics but was depressed on cells from eight of 12 uremic patients. Interferon-gamma above 5 units/ml was detected in less than 15% of plasma samples from controls, uremic patients or alcoholics on admission but was detected in four of eight samples from alcoholics at discharge (2-4 days after admission). The intensity of staining for class II antigens was depressed by more than 50% on lymphocytes from alcoholics and uremic patients. The expression of HLA class I and class II antigens was depressed whether uremic patients were hemodialyzed or not. The percentage of lymphocytes expressing CD16 was depressed in three of seven alcoholics and five of seven hemodialyzed patients. In contrast, the percentage of monocytes expressing CD16 was increased in six of seven hemodialyzed patients and three of five uremic patients not undergoing hemodialysis suggesting activation of monocytes in these patients. Plasma levels of beta 2-microglobulin were elevated by 61% in alcoholics, 50-fold in hemodialyzed patients, and 26-fold in nonhemodialyzed uremic patients.(ABSTRACT TRUNCATED AT 250 WORDS)
Jones
J M
JM
Laboratory of Metabolism and Molecular Biology, NIAAA, Rockville, Maryland 20852.
Veech
R L
RL
Abbasi
F
F
Yu
K
K
Yeralan
O
O
Briefel
G R
GR
Anderson
J
J
Mezey
E
E
D000328:Adult; D000437:Alcoholism / Q000276:immunology*; D000943:Antigens, Differentiation / Q000032:analysis*; D005260:Female; D006680:HLA Antigens / Q000032:analysis*; D006801:Humans; D007108:Immune Tolerance / Q000276:immunology; D007676:Kidney Failure, Chronic / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011961:Receptors, Fc / Q000032:analysis*; D017452:Receptors, IgG; D006435:Renal Dialysis; D014511:Uremia / Q000276:immunology*
D000943:Antigens, Differentiation; D006680:HLA Antigens; D011961:Receptors, Fc; D017452:Receptors, IgG