Experimental infections of a Phlebotomus perniciosus colony using different procedures.
Flies of two colonies of Phlebotomus perniciosus established in Italy and Spain respectively, were infected with a Leishmania infantum isolate from Spain. Only P. perniciosus from Spain was completely found susceptible to the infection. Experimental infections of P. perniciosus from Spain with the same L. infantum isolate were carried out using RPMI or PBS as infective feeding mediums. The results demonstrate that using PBS it is possible to re-feed a great quantity of infected flies.
Molina
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Servicio de Parasitología, Instituto de Salud Carlos III, Madrid, Spain.
D000818:Animals; D006224:Cricetinae; D004772:Entomology / Q000379:methods; D005260:Female; D006790:Host-Parasite Interactions; D007303:Insect Vectors / Q000469:parasitology*; D007558:Italy; D007893:Leishmania donovani / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D008647:Mesocricetus; D010274:Parasitology / Q000379:methods*; D010691:Phlebotomus / Q000469:parasitology*; D013030:Spain