title: Bacteriophage lambda P gene shows host killing which is not dependent on lambda DNA replication.
abstract: Bacteriophage lambda, having a mutation replacing glycine by glutamic acid at the 48th codon of cro, kills the host under N- conditions; we call this the hk mutation. In lambda N-N-cl-hk phage-infected bacteria, the late gene R is expressed to a significant level, phage DNA synthesis occurs with better efficiency, and the Cro activity is around 20% less, all compared to those in lambda N-N-cl-hk(+)-infected bacteria. Segments of lambda DNA from the left of pR to the right of tR2, carrying cro, cII, O, P, and the genes of the nin5 region from the above hk and hk+ phages, were cloned in pBR322. Studies with these plasmids and their derivatives having one or more of the lambda genes deleted indicate that the hk mutation is lethal only when a functional P gene is also present. When expression of P from pR is elevated, due to the deletion of tR1, host killing also occurs without the hk mutation. We conclude that the higher levels of P protein, produced either (1) when cro has the hk mutation or (2) when tR1 is deleted, are lethal to the host. We also show that due to the hk mutation, the Cro protein becomes partially defective in its negative regulation at pR, resulting in the expression of P to a lethal level even in the absence of N protein-mediated antitermination. This P protein-induced host killing depends neither on lambda DNA replication nor on any other gene functions of the phage.
authors.affiliation: Department of Biochemistry, Bose Institute, Calcutta, India.
authors.forename: S
authors.initials: S
authors.lastname: Maiti
authors.forename: M
authors.initials: M
authors.lastname: Mukhopadhyay
authors.forename: N C
authors.initials: NC
authors.lastname: Mandal
mesh_terms: D000595:Amino Acid Sequence; D010582:Bacteriophage lambda / Q000235:genetics / Q000472:pathogenicity*; D001483:Base Sequence; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D005804:Genes, Lethal; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000235:genetics; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*; D014779:Virus Replication
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins