[Restenosed growth of plaque cells from restenosed peripheral and coronary plaque material of the human in vitro: a correlation to animal experiment results following angioplasty].
In this study cells were isolated from primary-stenosing and re-stenosing tissue of human peripheral (extraction by Prof. Höfling and Dr. Bauriedel, Munich) and coronary arteries. The vast majority of plaque cells was identified as smooth muscle cells (SMC) by positive reaction with monoclonal antibodies against smooth muscle alpha-Actin. All SMC consisted of two different subpopulations, which were discriminated by different cell diameters: Relatively small cells (diameter: 18.0 +/- 4 microns, mean +/- SD) and large cells (diameter: 26.0 +/- 3 microms, mean +/- SD). Population doublings per day (PD/day) for SMC from re-stenosing lesions were significantly increased (0.60 +/- 0.13 PD/day, mean +/- SD) in comparison to SMC from primary-stenosing lesions (0.15 +/- 0.04 PD/day, mean +/- SD), p much less than 0.001). Thus, the increased growth of SMC from restenosing lesions in vitro might be the equivalent to the rapid progression of restenosing events after angioplasty in vivo. These results are in accordance with data from experimental studies after angioplasty.
Voisard
R
R
Physiologisches Institut I, Universität Tübingen.
Dartsch
P C
PC
Seitzer
U
U
Hannekum
A
A
Hombach
V
V
Betz
E
E
D000368:Aged; D000800:Angioplasty, Balloon; D015906:Angioplasty, Balloon, Coronary; D001161:Arteriosclerosis / Q000473:pathology* / Q000628:therapy*; D002455:Cell Division / Q000502:physiology*; D003324:Coronary Artery Disease / Q000473:pathology* / Q000628:therapy*; D046508:Culture Techniques; D004730:Endothelium, Vascular / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000473:pathology; D012008:Recurrence
Erhöhtes Wachstum von Plaquezellen aus restenosierendem periphren und koronaren Plaquematerial des Menschen in vitro: ein Korrelat zu tierexperimentellen Ergebnissen nach Angioplastie.