title: Matrix vesicle enzyme activity in endosteal bone following implantation of bonding and non-bonding implant materials.
abstract: The effect of bone bonding (KGy-Cera) and non-bone bonding (KGy-213) implant materials on primary mineralization was examined in endosteal bone repair following marrow ablation. Comparisons were made to determine implant effect on concentration and biochemical parameters of matrix vesicles, as contrasted to vesicles in normal bone healing. Matrix vesicle number was determined by high-resolution computerized morphometric analysis, and implant effect on the specific activity of alkaline phosphatase and phospholipase A2 was measured. Bone responses differed according to the composition of the implant material. The bone bonding implant in this study stimulated matrix vesicle formation, alkaline phosphatase specific activity, and, to a lesser extent, phospholipase A2 activity. The effect of the non-bonding implant on healing bone was of suppression of enzyme specific activities and reduced matrix vesicle production. The results indicate that the bone bonding implant material (KGy-Cera) promotes the initiation of primary mineralization, whereas failure of the KGy-213 to bond may be related to toxic materials that leach from the implant and inhibit the normal sequence of events in the mineralization cascade. The results also demonstrate that the implant materials alter the healing process distal to the injury site. Changes observed in the contralateral control limb mimic the changes observed in the injured limb, but at lower magnitude.
authors.affiliation: University of Texas Health Science Center at San Antonio.
authors.forename: T S
authors.initials: TS
authors.lastname: Marshall
authors.forename: Z
authors.initials: Z
authors.lastname: Schwartz
authors.forename: L D
authors.initials: LD
authors.lastname: Swain
authors.forename: D
authors.initials: D
authors.lastname: Amir
authors.forename: J
authors.initials: J
authors.lastname: Sela
authors.forename: U
authors.initials: U
authors.lastname: Gross
authors.forename: C
authors.initials: C
authors.lastname: Muller-Mai
authors.forename: B D
authors.initials: BD
authors.lastname: Boyan
mesh_terms: D000268:Adhesiveness; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001853:Bone Marrow / Q000201:enzymology; D001857:Bone Matrix / Q000201:enzymology*; D002516:Ceramics; D005109:Extracellular Matrix / Q000201:enzymology; D005898:Glass; D006132:Growth Plate / Q000201:enzymology; D008861:Microsomes / Q000201:enzymology; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D019736:Prostheses and Implants; D051381:Rats; D013977:Tibia; D014945:Wound Healing
chemical_list: D010741:Phospholipases A; D054467:Phospholipases A2; D000469:Alkaline Phosphatase