Microbial de-mannosylation and mannosylation of teicoplanin derivatives.
The single components of the teicoplanin complex, glycopeptide antibiotics active against Gram-positive bacteria, can be converted in the corresponding de-mannosyl derivatives by cultures of Nocardia orientalis NRRL 2450 or Streptomyces candidus NRRL 3218. Conversely, teicoplanin aglycone and other teicoplanin de-mannosyl derivatives can be converted in the corresponding teicoplanin mannosyl derivatives by cultures of Actinoplanes teichomyceticus ATCC 31121. The biological transformation yields are approximately 40% for de-mannosylation and 90% for mannosylation. The processes allow for the preparation of gram quantities of the de-mannosyl derivatives of teicoplanin and of teicoplanin mannosyl derivatives. De-mannosyl teicoplanin and teicoplanin mannosyl-pseudoaglycone were not amenable to preparation by either acidic or basic chemical hydrolysis.
Borghi
A
A
MMDRI, Centro Ricerche Lepetit, Gerenzano, Italy.
Ferrari
P
P
Gallo
G G
GG
Zanol
M
M
Zerilli
L F
LF
Lancini
G C
GC
D000192:Actinomycetales / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D006020:Glycopeptides / Q000378:metabolism; D008358:Mannose / Q000378:metabolism*; D009615:Nocardia / Q000378:metabolism*; D013302:Streptomyces / Q000378:metabolism*; D017334:Teicoplanin
D000900:Anti-Bacterial Agents; D006020:Glycopeptides; D017334:Teicoplanin; D008358:Mannose