title: Beta-thromboglobulin and polymorphonuclear leukocytes activation. (Effects on chemiluminescence, release of membrane bound calcium, NADPH-oxidase activity and membrane fluidity).
abstract: The aim of this paper was to evaluate the "in vitro" interaction between beta-thromboglobulin, one specific platelet release product and polymorphonuclear leukocytes. The effects of beta-thromboglobulin were tested on the following features of polymorphonuclear leukocytes activation: opsonized zymosan and phorbol myristate acetate stimulated chemiluminescence, release of membrane bound calcium, NADPH-oxidase activity, and membrane fluidity. Beta-thromboglobulin caused a decrease of the chemiluminescent response of polymorphonuclear leukocytes, using both agonists, with and without preincubation with polymorphonuclear leukocytes. We observed that beta-thromboglobulin caused a decrease also in the activity of NADPH-oxidase but not in the release of membrane bound calcium. Moreover beta-thromboglobulin caused a decrease of the polymorphonuclear leukocytes membrane fluidity. Our results suggest that the beta-thromboglobulin could play a role in the reciprocal interactions between platelets and polymorphonuclear leukocytes.
authors.affiliation: Institute of Rheumatology, Faculty of Medicine, University of Ancona, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Allegrezza-Giulietti
authors.forename: R
authors.initials: R
authors.lastname: Serretti
authors.forename: E
authors.initials: E
authors.lastname: Beccerica
authors.forename: G
authors.initials: G
authors.lastname: Ferretti
authors.forename: C
authors.initials: C
authors.lastname: Cervini
mesh_terms: D001792:Blood Platelets / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects*; D005454:Fluorescence Polarization; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D019255:NADPH Oxidases; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D009895:Opsonin Proteins; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000276:immunology; D001620:beta-Thromboglobulin / Q000494:pharmacology*
chemical_list: D009895:Opsonin Proteins; D001620:beta-Thromboglobulin; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium