[Effects of gossypol (+,-,+) on phorbol ester and A23187-stimulated prostaglandin synthesis by macrophages].
Previously, gossypol was found to have a wide range of cellular effects. In the present study, the effects of racemic, (-) and (+) gossypol on prostaglandin synthesis by mouse peritoneal macrophages were examined. The synthesis of prostacyclin, thromboxane A2 and prostaglandin F2 alpha was stimulated by phorbol-12-myristate-13-acetate (PMA), A23187 and arachidonic acid. Racemic, (-) and (+) gossypol dose-dependently inhibited PMA or A23187-stimulated prostaglandin synthesis, but failed to affect arachidonic acid stimulated prostaglandin synthesis. It is therefore possible that both (-) gossypol and (+) gossypol inhibit prostaglandin synthesis at the level of arachidonic acid release. Our studies point out two possibilities: 1) the inhibition of (+) and (-) gossypol on prostaglandin synthesis may not be the main mode of the contraceptive action of gossypol; 2) (+) gossypol, which also inhibits prostaglandin synthesis, may have antifertility effects.
Xiao
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Peking Union Medical College, Beijing.
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis*; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D015237:Dinoprost / Q000096:biosynthesis*; D006072:Gossypol / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D013237:Stereoisomerism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013929:Thromboxane B2 / Q000096:biosynthesis*
D000001:Calcimycin; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost; D006072:Gossypol; D013755:Tetradecanoylphorbol Acetate