mRNA variants encoding multiple forms of the high-affinity IgE receptor alpha subunit in transformed and nontransformed mast cells.
Multiple mRNA species encoding several predicted forms of the high-affinity IgE receptor alpha subunit (Fc epsilon RI-alpha) have been previously characterized from rat basophilic leukemia cells. Using the polymerase chain reaction procedure, we have extended these findings to show that one Fc epsilon RI-alpha mRNA variant, characterized by a 163-bp deletion within the coding sequence, exists in normal rat connective tissue mast cells as well as in both transformed and non transformed murine mast cell lines. In addition, a partial murine Fc epsilon RI-alpha genomic clone, spanning the internal-deletion sequence, has been identified, and from analysis of this sequence a mechanism of alternative pre-mRNA splicing is proposed. Finally, mRNA variants have been translated in a cell-free system and the protein products partially characterized.
Robertson
M W
MW
Medical Biology Institute, La Jolla, Calif.
Mehl
V S
VS
Richards
M L
ML
Liu
F T
FT
D000595:Amino Acid Sequence; D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D002461:Cell Line, Transformed / Q000737:chemistry; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000648:ultrastructure*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D011961:Receptors, Fc / Q000235:genetics*; D017455:Receptors, IgE; D012967:Sodium Dodecyl Sulfate; D014158:Transcription, Genetic
D000944:Antigens, Differentiation, B-Lymphocyte; D012333:RNA, Messenger; D011961:Receptors, Fc; D017455:Receptors, IgE; D012967:Sodium Dodecyl Sulfate