title: One hour in 1 ATA oxygen enhances rat alveolar macrophage chemiluminescence and fungal cytotoxicity.
abstract: The purpose of this study was to determine if 100% O2 would enhance rat pulmonary alveolar macrophage (PAM) oxidative killing of conidia of the fungus Neurospora crassa. First, we found that incubation in 100% O2 had no effect on conidia viability in the absence of PAM. We obtained resident PAM from nonpretreated anesthetized male Sprague-Dawley rats by bronchoalveolar lavage. Compared with similar air exposures we found that 1 h in vitro exposure of PAM to 100% O2 (1.0 atmosphere absolute) increased their killing of opsonized phagocytized conidia by 52%, without altering phagocytosis. A reactive oxygen killing mechanism is suggested because 1) 100% O2 increased PAM chemiluminescence (CL) by 60% both at rest and during 1 h of phagocytosis, and 2) PAM in 100% O2 killed albino conidia (lacking free radical-quenching carotenoids) 2.9 times more readily than they killed wild-type conidia. Compared with air, 100% O2 delayed the PAM respiratory burst time to peak by 18% but did not alter the burst maximal acceleration. The latter and the similar 60% increase in PAM CL in 100% O2 both at rest and during 1 h of phagocytosis suggests a nonenzymatic O2 enhancement of the respiratory burst. Changed timing of burst events in 100% O2 suggests early O2 toxicity. We conclude that 1 h in 100% O2 increases PAM free radical production and fungicidal activity.
authors.affiliation: Department of Physiology, John A. Burns School of Medicine, University of Hawaii, Honolulu 96822.
authors.forename: R M
authors.initials: RM
authors.lastname: Smith
authors.forename: P
authors.initials: P
authors.lastname: Mohideen
mesh_terms: D000818:Animals; D006931:Hyperbaric Oxygenation; D066298:In Vitro Techniques; D007700:Kinetics; D008163:Luminescent Measurements; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009492:Neurospora crassa; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology; D010100:Oxygen / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects*; D011650:Pulmonary Alveoli; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D010100:Oxygen