title: Sequence specificities of human fibroblast and neutrophil collagenases.
abstract: The sequence specificities of human fibroblast and neutrophil collagenases have been investigated by measuring the rate of hydrolysis of 60 synthetic oligopeptides covering the P4 through P'5 subsites of the substrate. The choice of peptides was patterned after both known cleavage sites in noncollagenous proteins and potential cleavage sites (those containing Gly-Ile-Ala, Gly-Leu-Ala, or Gly-Ile-Leu sequences) found in types I, II, III, and IV collagens. The initial rate of hydrolysis of the P1-P'1 bond of each peptide has been measured under first-order conditions ([SO] much less than KM), and kcat/KM values have been calculated from the initial rates. The amino acids in subsites P4 through P'4 all influence the hydrolysis rates for both collagenases. However, the effects of substitutions at each site are distinctive and are consistent with the view that human fibroblast and neutrophil collagenases are homologous but nonidentical enzymes. For peptides with unblocked NH2 and COOH termini, occupancy of subsites P3 through P'3 is necessary for rapid hydrolysis. Compared with the alpha 1(I) cleavage sequence, none of the substitutions investigated at subsites P3, P2, and P'4 produces markedly improved substrates. In contrast, many substitutions at subsites P1, P'1, and P'2 improve specificity. The preferences of both collagenases for alanine in subsite P1 and tryptophan or phenylalanine in subsite P'2, is noteworthy. Human neutrophil collagenase accommodates aromatic residues in subsite P'1 much better than human fibroblast collagenase. The subsite preferences observed for human fibroblast collagenase in these studies agree well with the residues found at cleavage sites in noncollagenous substrates. However, the sequence specificities of these collagenases cannot explain the failure of these enzymes to hydrolyze many potentially cleavable but apparently protected sites in intact collagens. This represents additional support for the notion that the local structure of collagen is important in determining the location of collagenase cleavage sites.
authors.affiliation: Department of Chemistry, Florida State University, Tallahassee 32306.
authors.forename: S
authors.initials: S
authors.lastname: Netzel-Arnett
authors.forename: G B
authors.initials: GB
authors.lastname: Fields
authors.forename: H
authors.initials: H
authors.lastname: Birkedal-Hansen
authors.forename: H E
authors.initials: HE
authors.lastname: Van Wart
authors.forename: G
authors.initials: G
authors.lastname: Fields
mesh_terms: D000595:Amino Acid Sequence; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D003012:Microbial Collagenase / Q000378:metabolism*; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000201:enzymology*; D010455:Peptides / Q000138:chemical synthesis / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D010455:Peptides; D003094:Collagen; D003012:Microbial Collagenase