title: The value of platelet transfusions as preparation for kidney transplantation.
abstract: The role of platelet transfusion as a preparative method for kidney transplantation is still a matter of debate. Two groups of 28 male patients transplanted between 1983 and 1988, paired for age, date of transplant, absence of anti-HLA antibody and immunosuppressive therapy have been compared. Group I was given 5 purified platelet transfusions at 1-week intervals before transplantation. Each transfusion contained 7.6 x 10(6) platelets contaminated by less than 1 leukocyte in 10(5) platelets. Group II received from 3 to 5 whole blood transfusions. In all cases it was a first transplant from cadaveric donors and previously untransfused patients before entering the protocol. No patient in group I developed cytotoxic antibodies. Acute tubular necrosis occurred with the same incidence in group I and in group II but was more severe and longer in group I, requiring hemodialysis in 62.5% and only 22% in group II. ATN was significantly associated with graft loss in group I (P less than 0.05). The total number of rejections and the number of patients undergoing rejection were not significantly different in both groups. However, the intensity of rejection was significantly higher in group I with 41% (21/51) of severe or irreversible rejections versus 9/46 (19.5%) in group II (P less than 0.05). The first rejection occurred significantly earlier in group I than in group II since 75% of the first rejection episodes occurred in the first 10 days versus 38% in group II (P less than 0.02) with a mean delay of 12.8 +/- 3.2 and 19.10 +/- 3.3 days, respectively. Although platelet transfusions are devoid of leukocytes the incidence of CMV infection was not significantly different in both groups: 57% in group I and 68% in group II. Purified platelet transfusions did not induce humoral immunization but lack of sensitization does not imply indefinite graft prolongation. Because platelets do not carry class II antigens, purified platelets transfusions represent a useful model to analyze the role of class I antigens alone in the induction of unresponsiveness in organ transplantation.
authors.affiliation: Nephro-Urology, Transplantation, and Clinical Immunology Unit, INSERM U 80, C.N.R.S. U1 1177, University Claude Bernard, Lyon, France.
authors.forename: C
authors.initials: C
authors.lastname: Pouteil-Noble
authors.forename: H
authors.initials: H
authors.lastname: Betuel
authors.forename: P
authors.initials: P
authors.lastname: Raffaele
authors.forename: F
authors.initials: F
authors.lastname: Robert
authors.forename: J M
authors.initials: JM
authors.lastname: Dubernard
authors.forename: J L
authors.initials: JL
authors.lastname: Touraine
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis; D001803:Blood Transfusion / Q000592:standards*; D003587:Cytomegalovirus / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000209:etiology; D006084:Graft Rejection; D006085:Graft Survival; D006801:Humans; D007668:Kidney / Q000502:physiology; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D017713:Platelet Transfusion; D065227:Transfusion Reaction
chemical_list: D000914:Antibodies, Viral