The kinase insert domain of colony stimulating factor-1 receptor is dispensable for CSF-1 induced phosphatidylcholine hydrolysis.
Mouse NIH 3T3 fibroblasts transfected with human colony stimulating factor-1 receptor produced diacylglycerol in response to CSF1 and this correlated with elevated phosphatidylcholine hydrolyzing activity measured in an in vitro assay. Treatment of cells with the isoflavone derivative genistein attenuated PC hydrolysis in vitro suggesting a role for CSF1R tyrosine kinase activity. A CSF1R mutant lacking 67 amino acids of the kinase insert domain, which may affect the association of receptor with certain substrates, stimulated PC hydrolysis in response to CSF1. Coupling to PC hydrolysis is likely a general property of CSF1R and the kinase insert domain is dispensable for this activity.
Choudhury
G G
GG
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284-7762.
Sylvia
V L
VL
Wang
L M
LM
Pierce
J
J
Sakaguchi
A Y
AY
D000818:Animals; D004252:DNA Mutational Analysis; D019833:Genistein; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D051379:Mice; D010713:Phosphatidylcholines / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000502:physiology*; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000502:physiology*; D011994:Recombinant Proteins; D015398:Signal Transduction; D013329:Structure-Activity Relationship; D014162:Transfection
D007529:Isoflavones; D010713:Phosphatidylcholines; D011994:Recombinant Proteins; D016173:Macrophage Colony-Stimulating Factor; D019833:Genistein; D011505:Protein-Tyrosine Kinases; D016186:Receptor, Macrophage Colony-Stimulating Factor