PCR-SSO typing for DR4-Dw subtypes: application to unrelated bone marrow transplant donor selection.
Thirty-seven DR4-positive patient-unrelated bone marrow donor pairs previously DR/DQ restriction fragment length polymorphism (RFLP) typed and tested in mixed lymphocyte culture (MLC), have been DR4-Dw subtyped retrospectively using sequence specific oligonucleotide probes. We found that DR4-Dw subtyping substantially increased the accuracy of pre-MLC matching and could potentially accelerate donor searches by avoiding unnecessary MLC tests on Dw-mismatched donors.
Clay
T M
TM
United Kingdom Transplant Service, Bristol.
Howard
M R
MR
Bidwell
J L
JL
Bidwell
E A
EA
Raymond
P A
PA
Evans
J E
JE
Bradley
B A
BA
D016026:Bone Marrow Transplantation / Q000276:immunology*; D015342:DNA Probes; D015804:HLA-DR4 Antigen / Q000145:classification / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D007959:Lymphocyte Culture Test, Mixed; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D014019:Tissue Donors
D015342:DNA Probes; D015804:HLA-DR4 Antigen; D009838:Oligodeoxyribonucleotides