title: Primary structures of two subunits of NADH: ubiquinone reductase from Neurospora crassa concerned with NADH-oxidation. Relationship to a soluble NAD-reducing hydrogenase of Alcaligenes eutrophus.
abstract: The primary structures of the nuclear-encoded 51 kDa and 78 kDa subunits of the respiratory chain NADH: ubiquinone reductase (complex I) from Neurospora crassa mitochondria were determined by sequencing cDNA and the N-terminus of the mature proteins. Both subunits are related to the soluble NAD-reducing hydrogenase of the bacterium Alcaligenes eutrophus. Sequence comparison between these subunits, the corresponding subunits of the bovine complex I and the bacterial NAD-reducing hydrogenase further confirms the binding sites of NAD(H), FMN and three iron-sulfur clusters.
authors.affiliation: Institut für Biochemie, Heinrich-Heine-Universität Düsseldorf, F.R.G.
authors.forename: D
authors.initials: D
authors.lastname: Preis
authors.forename: U
authors.initials: U
authors.lastname: Weidner
authors.forename: C
authors.initials: C
authors.lastname: Conzen
authors.forename: J E
authors.initials: JE
authors.lastname: Azevedo
authors.forename: U
authors.initials: U
authors.lastname: Nehls
authors.forename: D
authors.initials: D
authors.lastname: Röhlen
authors.forename: J
authors.initials: J
authors.lastname: van der Pas
authors.forename: U
authors.initials: U
authors.lastname: Sackmann
authors.forename: R
authors.initials: R
authors.lastname: Schneider
authors.forename: S
authors.initials: S
authors.lastname: Werner
mesh_terms: D000421:Alcaligenes / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002417:Cattle; D005486:Flavin Mononucleotide / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D009243:NAD / Q000378:metabolism; D016660:NAD(P)H Dehydrogenase (Quinone); D009245:NADH Dehydrogenase / Q000737:chemistry / Q000235:genetics*; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D011808:Quinone Reductases / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D013455:Sulfur / Q000378:metabolism
chemical_list: D009243:NAD; D013455:Sulfur; D005486:Flavin Mononucleotide; D007501:Iron; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases; D009245:NADH Dehydrogenase