The clearance and degradation of oligodeoxynucleotides following intravenous injection into rabbits.
A 32P-labeled oligodeoxynucleotide of 20 bases was found to be quite stable in freshly drawn rabbit or human blood and no degradation was apparent within 2 hr. In contrast, following injection into rabbits, the same oligodeoxynucleotide was cleared from the blood rapidly and at least 17% was completely degraded within 5 min. After 90 min, 16% of the oligonucleotide was found largely intact in the urine. At this time, radioisotope was also found in all the solid tissues tested but probably not as intact oligonucleotide.
Goodchild
J
J
Worcester Foundation for Experimental Biology, Shrewsbury, MA 01545.
Kim
B
B
Zamecnik
P C
PC
D000818:Animals; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D007275:Injections, Intravenous; D007700:Kinetics; D008657:Metabolic Clearance Rate; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage / Q000378:metabolism* / Q000493:pharmacokinetics; D011817:Rabbits; D013997:Time Factors
D009838:Oligodeoxyribonucleotides