Characterization of products of TY1-mediated reverse transcription in Saccharomyces cerevisiae.
Transposition of the yeast transposable element, Ty, has been shown to require a reverse transcription process. By analysing the extrachromosomal Ty-specific nucleic acid molecules associated with overproduced Ty virus-like particles (Ty-VLPs), we identified several reverse transcribed cDNA strands. Most of them resemble the characteristic intermediates of the reverse transcription process described for authentic retroviruses: a (-) strong-stop DNA strand covalently bound to an RNA primer, two elongated (-) strands with one or two long terminal repeat (LTR) sequences and a (+) strong-stop DNA. Surprisingly, complete (+) strands and full-length linear duplex Ty DNA could not be detected. The structural features of two additional (+) strands may indicate some differences between the mechanisms of (+) strand synthesis in Ty and other retrotransposons or retroviruses.
Müller
F
F
Institut für Mikrobiologie, Universität Düsseldorf, Federal Republic of Germany.
Laufer
W
W
Pott
U
U
Ciriacy
M
M
D001483:Base Sequence; D004251:DNA Transposable Elements; D004271:DNA, Fungal / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013728:Terminator Regions, Genetic / Q000235:genetics; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D004271:DNA, Fungal