Further improvement in protecting method for the oligonucleotide synthesis in terms of a cellulose acetate derivative as a polymer support.
For further improvement in the investigation to utilize a cellulose acetate derivative as a novel type of polymer-support for the synthesis of oligonucleotides, the investigations on utilizing another spacer; on protecting groups for O6-position of guanosine unit, ribothymidine, and pseudouridine; and on a novel protecting group for the introduction of phosphate function at 5'-terminal position, targeting the syntheses of 13-mer, ApApGpGpApApApApUpUpApUpG, 11-mer, pCpUpCpGpUpCpCpApCpCpA, and 12-mer, UpCpCpGpGprTp- psipCpGpApUpU, found in the partial structures of a yeast tRNA(Ala), will be described in detail.
Kamaike
K
K
Laboratory of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Tokyo College of Pharmacy, Japan.
Ogawa
T
T
Inoue
Y
Y
Ishido
Y
Y
D001483:Base Sequence; D002482:Cellulose / Q000031:analogs & derivatives; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D011560:Pseudouridine; D012344:RNA, Transfer, Ala / Q000138:chemical synthesis; D012441:Saccharomyces cerevisiae
D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D012344:RNA, Transfer, Ala; D011560:Pseudouridine; C005062:acetylcellulose; D002482:Cellulose