Adenovirus E1A activation domain binds the basic repeat in the TATA box transcription factor.
The adenovirus large E1A protein is a potent activator of transcription. We use several different experimental approaches to demonstrate that the large E1A protein binds specifically and stably to the TATA box-binding factor (TFIID), the general polymerase II transcription factor that initiates assembly of transcription complexes. Sedimentation velocity centrifugation revealed that TFIID and E1A form a heterodimer in vitro. We demonstrate that the activation domain of E1A (conserved region 3) binds to TFIID. E1A interacts with a 51 residue region from the conserved C-terminal domain of TFIID that includes a repeat of basic residues between the homologous direct repeats of TFIID. Analysis of TFIID binding by various E1A mutants indicates that TFIID binding is necessary, although not sufficient, for E1A transactivation.
Lee
W S
WS
Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024-1570.
Kao
C C
CC
Bryant
G O
GO
Liu
X
X
Berk
A J
AJ
D000256:Adenoviridae / Q000235:genetics*; D017456:Adenovirus Early Proteins; D000595:Amino Acid Sequence; D001665:Binding Sites / Q000502:physiology; D015153:Blotting, Western; D004926:Escherichia coli / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016385:TATA Box / Q000502:physiology; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology; D014616:Vaccinia virus / Q000235:genetics
D017456:Adenovirus Early Proteins; D046911:Macromolecular Substances; D009856:Oncogene Proteins, Viral; D011993:Recombinant Fusion Proteins; D035362:Transcription Factor TFIID; D014157:Transcription Factors