[A study of protein engineering for human cardionatrin. II. Construction of two new human cardionatrin analog genes, RH-2 & RH-3 by intragenic recombination in vitro].
Two new analog genes of the alpha-human atrial natriuretic polypeptide (alpha-hANP) were constructed by accurate intragenic recombination of analog RH-1 gene with ANP gene in vitro, both of which had been synthesized and reported. One recombined analog gene, RH-2, was designed to contain an equivalent of the alpha-hANP gene, capping the peptidase inhibitor SQ20881 sequence at its 5'end. The other analog gene, RH-3, was designed to contain an equivalent of the alpha-hANP gene, wedging in two proline codons CCG & CCG at the 3' end. The recombinants bearing the genes, bacteriophage RH-2/M13mp18 & RH-3/M13mp18, were identified by DNA domain combination analysis--in situ hybridization and dot blotting with three probes matching three specific DNA domains, and by restriction analysis. The DNA sequences of RH-2 and RH-3 were confirmed by the dideoxynucleotide chain termination method.
Ren
H
H
Institute of Basic Medical Sciences, Beijing.
D000595:Amino Acid Sequence; D009320:Atrial Natriuretic Factor / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D005796:Genes / Q000235:genetics*; D005813:Genes, Synthetic / Q000235:genetics*; D008969:Molecular Sequence Data; D015202:Protein Engineering; D011995:Recombination, Genetic
D009320:Atrial Natriuretic Factor; D004247:DNA