title: Rapid deprotection procedures for synthetic oligonucleotides.
abstract: Two new rapid procedures for the full deprotection of synthetic oligonucleotides has been developed. We have successfully used the mixture of ethanolamine and ethanol (1:1) or pure ethanolamine for deprotection of oligonucleotides, prepared by different methods. In the case of oligonucleotides prepared by commonly used beta-cyanoethyl phosphoramidite and H-phosphonates method deprotection takes half an hour at 70 degrees C. We have found also that mixture of hydrazine, ethanolamine and methanol (1:3:3, v/v/v) can serve as a very efficient reagent for deprotection of oligonucleotides, prepared by beta-cyanoethyl phosphoramidite method with isopropoxyacetyl protecting group for cytosine residues. In this case deprotection time is 12-17 min at room temperature.
authors.affiliation: Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.
authors.forename: N N
authors.initials: NN
authors.lastname: Polushin
authors.forename: I N
authors.initials: IN
authors.lastname: Pashkova
authors.forename: V A
authors.initials: VA
authors.lastname: Efimov
mesh_terms: D002621:Chemistry / Q000379:methods; D007202:Indicators and Reagents; D009841:Oligonucleotides / Q000138:chemical synthesis*; D063065:Organophosphonates; D010710:Phosphates
chemical_list: D007202:Indicators and Reagents; D009841:Oligonucleotides; D063065:Organophosphonates; D010710:Phosphates