title: CD3-induced T-cell proliferation and interleukin-2 secretion is modulated by the CD45 antigen.
abstract: In the present study we have investigated the effect of CD45, CD45RA and CD45RO monoclonal antibodies (MoAbs) on the CD3 receptor-mediated proliferation of human T lymphocytes. It is shown that CD3-induced proliferation of purified resting T cells and quiescent T lymphoblasts (QTL) is promoted via all of the investigated CD45-associated epitopes. It is also shown that the CD45 molecules are required to be cross-linked for costimulation. The MoAbs enhance the interleukin-2 (IL-2) production of CD3-stimulated QTL. The elevation of the IL-2 production correlates with the increase in CD3-induced cell proliferation suggesting that the CD45-driven regulation of T lymphocyte activation is linked to the IL-2 pathway.
authors.affiliation: Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Szeged.
authors.forename: T
authors.initials: T
authors.lastname: Oravecz
authors.forename: E
authors.initials: E
authors.lastname: Monostori
authors.forename: E
authors.initials: E
authors.lastname: Kurucz
authors.forename: L
authors.initials: L
authors.lastname: Takács
authors.forename: I
authors.initials: I
authors.lastname: Andó
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000502:physiology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D017252:CD3 Complex; D006649:Histocompatibility Antigens / Q000502:physiology*; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D017493:Leukocyte Common Antigens; D008213:Lymphocyte Activation; D051379:Mice; D010766:Phosphorylation; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014443:Tyrosine / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D006649:Histocompatibility Antigens; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D014443:Tyrosine; D017493:Leukocyte Common Antigens