title: HU and integration host factor function as auxiliary proteins in cleavage of phage lambda cohesive ends by terminase.
abstract: HU and integration host factor (IHF) are small, basic heterodimeric DNA-binding proteins which participate in transcription initiation, DNA replication, and recombination. We constructed isogenic Escherichia coli strains in which HU, IHF, or both proteins were absent. Bacteriophage lambda did not grow in hosts lacking both HU and IHF. Phage DNA replication and late gene transcription were normal in the double mutants, but packaging of lambda DNA was defective. Mature phage DNA molecules were absent, indicating that terminase was unable to linearize lambda DNA. Phage variants carrying a small substitution near cos or the ohm1 mutation in the terminase gene, Nul, formed plaques on HU- IHF- strains. We propose that HU or IHF is required to establish the higher-order DNA-protein structure at cos that is the substrate for lambda terminase.
authors.affiliation: Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
authors.forename: I
authors.initials: I
authors.lastname: Mendelson
authors.forename: M
authors.initials: M
authors.lastname: Gottesman
authors.forename: A B
authors.initials: AB
authors.lastname: Oppenheim
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D010582:Bacteriophage lambda / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D003850:Deoxyribonuclease I; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D034982:Integration Host Factors; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D034982:Integration Host Factors; D012333:RNA, Messenger; C040330:histone-like protein HU, bacteria; D004706:Endodeoxyribonucleases; C030894:terminase; D003850:Deoxyribonuclease I