title: An unusual repetitive gene family in Theileria parva which is stage-specifically transcribed.
abstract: Tpr1 is a repetitive DNA element from Theileria parva which has previously been shown to be of value in strain characterisation. Further characterisation, described here, has shown that Tpr1 is present in long tandem arrays. The sequence of 8.1 kb from one end of an array has been determined. The sequence showed that Tpr1 is a 1.44-kb element which contains an ORF extending from its 5' end to the 3' end. The sequenced region contains 4 large ORFs; 2 of these consisted only of Tpr1 whilst the third consisted of Tpr1 and a 0.55-kb element (Tpr2) located 5' of Tpr1. The largest ORF consisted of Tpr1 plus Tpr2 as well as an additional 420-bp element (Tpr3) 5' of Tpr2, thus a continuous ORF arranged 5'-Tpr3-Tpr2-Tpr1-3' was formed. This ORF potentially encodes a 795 amino acid polypeptide commencing at an ATG close to the 5' end. In contrast the first in frame ATGs in the other 3 ORFs are at least 417 bp from the 5' end. Southern analysis confirmed that the sequenced region was typical of the rest of the Tpr array(s). Transcripts containing both Tpr3 and Tpr1 were detected in the piroplasm but not the schizont stages of the life cycle.
authors.affiliation: Department of Biochemistry, University of Cambridge, U.K.
authors.forename: H A
authors.initials: HA
authors.lastname: Baylis
authors.forename: S K
authors.initials: SK
authors.lastname: Sohal
authors.forename: M
authors.initials: M
authors.lastname: Carrington
authors.forename: R P
authors.initials: RP
authors.lastname: Bishop
authors.forename: B A
authors.initials: BA
authors.lastname: Allsopp
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D016797:Theileria parva / Q000235:genetics* / Q000254:growth & development; D014158:Transcription, Genetic
chemical_list: D016054:DNA, Protozoan