Formation and selection of intergenogroup reassortants during cell culture adaptation of rotaviruses from dually infected subjects.
A previous study showed that intergenogroup reassortants of human rotaviruses can persist in nature (R.L. Ward, O. Nakagomi, D.R. Knowlton, M.M. McNeal, T. Nakagomi, J.D. Clemens, D.A. Sack, and G.M. Schiff, J. Virol. 64:3219-3225, 1990), but the mechanisms involved in their formation and selection had not been determined. In this study it was shown that, during cell culture adaptation of rotaviruses belonging to different genogroups from stools of dually infected subjects, intergenogroup reassortants were formed and selected, presumably mimicking the processes that occur in nature.
Ward
R L
RL
Division of Clinical Virology, James N. Gamble Institute of Medical Research, Cincinnati, Ohio 45219.
Nakagomi
O
O
Knowlton
D R
DR
McNeal
M M
MM
Nakagomi
T
T
Huda
N
N
Clemens
J D
JD
Sack
D A
DA
D000818:Animals; D000914:Antibodies, Viral; D002478:Cells, Cultured; D006801:Humans; D009500:Neutralization Tests; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D012401:Rotavirus / Q000235:genetics* / Q000276:immunology; D012400:Rotavirus Infections / Q000276:immunology / Q000382:microbiology*
D000914:Antibodies, Viral; D012367:RNA, Viral