Effects of adduct formation derived from ethylene dibromide on DNA conformation.
Spectral properties of DNA oligomers containing the single modified guanine, S-[2-(N7-guanyl)ethyl]-glutathione, the major adduct derived from 1,2-dibromoethane, were investigated using UV, CD, and NMR. Two palindromic hexamers, d(ATGCAT) and d(ATCGAT), did not form a duplex with guanine bases modified. When the non self-complementary heptamer, d(CATGCCT), was modified at the single guanine, it formed a duplex with its normal complement d(AGGCATG), although the melting temperature was lowered. However, no duplex formation was observed when a non complementary base other than cytosine was placed in d(AGGXATG), suggesting that non Watson-Crick type base pairs are not stabilized by formation of this adduct.
Oida
T
T
Center in Molecular Toxicology, Vanderbilt University, Nashville, TN 37232.
Humphreys
W G
WG
Guengerich
F P
FP
D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D004249:DNA Damage; D015946:Ethylene Dibromide / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D013056:Spectrophotometry, Ultraviolet
D015946:Ethylene Dibromide; D004247:DNA