title: Polymerase chain reaction amplification of the genome of infectious bronchitis virus.
abstract: The polymerase chain reaction (PCR) was used to amplify a portion of the genome of infectious bronchitis virus (IBV). Two synthetic primers that annealed to segments of the IBV genome in the matrix and nucleocapsid genes facilitated PCR amplification of a 1020-base sequence. Amplification was successful using template DNA from an IBV sequence-containing plasmid and using copy DNA created by reverse transcription of IBV genomic RNA. The PCR product was the expected size and had the expected nucleotide sequence.
authors.affiliation: Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30605.
authors.forename: J R
authors.initials: JR
authors.lastname: Andreasen
authors.forename: M W
authors.initials: MW
authors.lastname: Jackwood
authors.forename: D A
authors.initials: DA
authors.lastname: Hilt
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens; D004279:DNA, Viral / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D005784:Gene Amplification; D001351:Infectious bronchitis virus / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000737:chemistry*; D013698:Templates, Genetic; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral