CD3-induced T-cell proliferation and interleukin-2 secretion is modulated by the CD45 antigen.
In the present study we have investigated the effect of CD45, CD45RA and CD45RO monoclonal antibodies (MoAbs) on the CD3 receptor-mediated proliferation of human T lymphocytes. It is shown that CD3-induced proliferation of purified resting T cells and quiescent T lymphoblasts (QTL) is promoted via all of the investigated CD45-associated epitopes. It is also shown that the CD45 molecules are required to be cross-linked for costimulation. The MoAbs enhance the interleukin-2 (IL-2) production of CD3-stimulated QTL. The elevation of the IL-2 production correlates with the increase in CD3-induced cell proliferation suggesting that the CD45-driven regulation of T lymphocyte activation is linked to the IL-2 pathway.
Oravecz
T
T
Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Szeged.
Monostori
E
E
Kurucz
E
E
Takács
L
L
Andó
I
I
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000502:physiology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D017252:CD3 Complex; D006649:Histocompatibility Antigens / Q000502:physiology*; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D017493:Leukocyte Common Antigens; D008213:Lymphocyte Activation; D051379:Mice; D010766:Phosphorylation; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014443:Tyrosine / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D006649:Histocompatibility Antigens; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D014443:Tyrosine; D017493:Leukocyte Common Antigens