Molecular cloning, chromosomal mapping, and expression of the cDNA for p107, a retinoblastoma gene product-related protein.
p107 is a cellular protein that forms specific complexes with adenovirus E1A and SV40 large T antigen (T). The genetics of the p107-T/E1A interaction as well as other features of this protein suggests that p107 shares functional properties with the tumor suppressor product, RB. A partial cDNA for human p107 has been cloned. Its sequences map to 20q11.2 and encode a 936 residue protein. Comparison analysis of the p107 protein sequence reveals a major region of RB homology extending over 564 residues. This region in RB is essential to its growth-controlling function. Sequences outside of these two regions are largely unique to each protein. The p107 and RB homology regions can independently bind to T and E1A. Thus, these two proteins display similarities of structure that may, at least in part, explain their known functional similarities and suggest a generic function for p107 in cell cycle regulation.
Ewen
M E
ME
Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115.
Xing
Y G
YG
Lawrence
J B
JB
Livingston
D M
DM
D017456:Adenovirus Early Proteins; D000595:Amino Acid Sequence; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism*; D001483:Base Sequence; D002874:Chromosome Mapping; D002890:Chromosomes, Human, Pair 20; D003001:Cloning, Molecular; D016147:Genes, Tumor Suppressor; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D016160:Retinoblastoma Protein / Q000737:chemistry*; D050720:Retinoblastoma-Like Protein p107; D016415:Sequence Alignment
D017456:Adenovirus Early Proteins; D000952:Antigens, Polyomavirus Transforming; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D009856:Oncogene Proteins, Viral; D011506:Proteins; C493844:RBL1 protein, human; D016160:Retinoblastoma Protein; D050720:Retinoblastoma-Like Protein p107