The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells.
The contribution of the E6 and E7 open reading frames of human papillomavirus type 6b (HPV6b) and HPV16 to immortalization of human keratinocytes was evaluated by using amphotropic recombinant retroviruses. The HPV16 E7 gene could immortalize primary human keratinocytes without the cooperation of the viral E6 gene; however, E6 was able to contribute significantly to the efficiency of the E7 immortalizing function. Infection of HFE cells with retroviruses carrying the 16E6, 6bE6, or 6bE6E7 open reading frame did not result in immortalization.
Halbert
C L
CL
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104-2092.
Demers
G W
GW
Galloway
D A
DA
D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D005814:Genes, Viral; D005822:Genetic Vectors; D006801:Humans; D015603:Keratinocytes; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D016366:Open Reading Frames; D027383:Papillomaviridae / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D011995:Recombination, Genetic; D012190:Retroviridae / Q000235:genetics; D012867:Skin / Q000166:cytology
D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16