title: Direct double-stranded DNA sequencing with baculovirus genomes.
abstract: Double-stranded DNA sequencing with the modified T7 DNA polymerase (Sequenase) was performed directly with nuclear polyhedrosis virus DNA genomes. The conditions were optimized to allow for a rapid and unambiguous sequence analysis of nuclear polyhedrosis virus genomes.
authors.affiliation: Department of Biological Sciences, University of Notre Dame, Indiana 46556.
authors.forename: H H
authors.initials: HH
authors.lastname: Wang
authors.forename: M J
authors.initials: MJ
authors.lastname: Fraser
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D004270:DNA, Circular; D004279:DNA, Viral / Q000032:analysis*; D005814:Genes, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics* / Q000382:microbiology
chemical_list: D004251:DNA Transposable Elements; D004270:DNA, Circular; D004279:DNA, Viral