Trigonal crystals of porin from Escherichia coli.
Trigonal crystals of the integral membrane protein porin from Escherichia coli have been grown and characterized. They belong to space group P321 with unit cell constants a = b = LL8.4, c = 52.7 A, alpha = beta = 90 degrees, gamma = 120 degrees. The crystals grow as well-defined hexagonal prisms to a size of 0.25 mm in all dimensions, and diffract to 2.7 A. The molecular symmetry coincides with 3-fold crystallographic symmetry, giving two trimers per unit cell (1 monomer/asymmetric unit). This corresponds to VM = 2.9 A3/Da. Native X-ray data to 3.0 A resolution have been collected on a FAST area detector and a search for heavy atom derivatives is underway.
Pauptit
R A
RA
Department of Microbiology, University of Basel, Switzerland.
Zhang
H
H
Rummel
G
G
Schirmer
T
T
Jansonius
J N
JN
Rosenbusch
J P
JP
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D003460:Crystallization; D004926:Escherichia coli / Q000032:analysis*; D018272:Porins
D001425:Bacterial Outer Membrane Proteins; D018272:Porins