Serotonin-stimulated phospholipase A2 and collagenase activation in chondrocytes from human osteoarthritic articular cartilage.
We have previously described several receptors on the chondrocyte membrane. In an attempt to further characterize the coupling mechanisms of serotoninergic receptors, here we examined the involvement of serotonin in the phospholipase A2 activity. Serotonin dose-dependently stimulated phospholipase A2. This activation enhanced collagenase type II activity and had no effect on proteoglycanase activity.
Richard
M
M
Department of General and Medical Biochemistry, INSERM-CNRS U 189 Lyon-Sud Medical School, Oullins, France.
Vignon
E
E
Peschard
M J
MJ
Broquet
P
P
Carret
J P
JP
Louisot
P
P
D000368:Aged; D002358:Cartilage, Articular / Q000201:enzymology* / Q000473:pathology; D010450:Endopeptidases / Q000378:metabolism; D004789:Enzyme Activation; D006801:Humans; D008666:Metalloendopeptidases; D003012:Microbial Collagenase / Q000096:biosynthesis* / Q000378:metabolism; D008875:Middle Aged; D010003:Osteoarthritis / Q000201:enzymology*; D010741:Phospholipases A / Q000096:biosynthesis* / Q000378:metabolism; D054467:Phospholipases A2; D012701:Serotonin / Q000502:physiology*
D012701:Serotonin; D010741:Phospholipases A; D054467:Phospholipases A2; D010450:Endopeptidases; C022474:proteoglycan-degrading metalloendopeptidases; D008666:Metalloendopeptidases; D003012:Microbial Collagenase