Kinetic and immunologic evidence for the absence of glucose-6-phosphatase in early human chorionic villi and term placenta.
The existence of the enzyme glucose-6-phosphatase (G6Pase) in early and term human placenta was investigated by comparing the characteristics of placental microsomal glucose 6-phosphate (G6P) hydrolytic activity and liver G6Pase. Placental microsomes exhibited similar apparent Km values for G6P and beta-glycerophosphate in intact and deoxycholate-treated microsomes, heat stability at acidic pH, low latency of mannose 6-phosphate hydrolysis, very low activity of pyrophosphate: glucose phosphotransferase, and undetectable [U-14C]G6P transport into the placental microsomes, all of which indicated that specific G6Pase activity does not exist in placenta. Immunological evidence of the absence of both 36.5 kDa and T2 proteins, which represent the G6Pase catalytic protein and the phosphate/pyrophosphate transporter protein, respectively, confirmed that early and term human placenta are devoid of the multicomponent G6Pase enzyme.
Barash
V
V
Department of Biochemistry, Hadassah University Hospital, Jerusalem, Israel.
Riskin
A
A
Shafrir
E
E
Waddell
I D
ID
Burchell
A
A
D000818:Animals; D001692:Biological Transport; D015153:Blotting, Western; D002824:Chorionic Villi / Q000201:enzymology*; D005865:Gestational Age; D005952:Glucose-6-Phosphatase / Q000276:immunology / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005958:Glucosephosphates / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000201:enzymology; D007700:Kinetics; D008360:Mannosephosphates / Q000378:metabolism; D008861:Microsomes / Q000201:enzymology; D008862:Microsomes, Liver / Q000201:enzymology; D010920:Placenta / Q000201:enzymology*; D051381:Rats; D013379:Substrate Specificity
D005958:Glucosephosphates; D008360:Mannosephosphates; C027693:mannose-6-phosphate; D005954:Glucosephosphate Dehydrogenase; D005952:Glucose-6-Phosphatase