RNA editing of sorghum mitochondrial atp6 transcripts changes 15 amino acids and generates a carboxy-terminus identical to yeast.
Sequencing of sorghum mitochondrial atp6 cDNA clones revealed 19 C-to-U transcript editing events within a 756 bp-conserved core gene; three were silent and 16 resulted in 15 amino acid changes. Only one edit, which was silent, was found in the 381 bp amino-extension to the core gene. Eleven of the 15 changed amino acids were identical with or else represented conservative changes compared to yeast atp6. Editing of a CAA codon to TAA truncates the carboxy-terminus to a position identical to that of yeast. The frequency of editing at sites which change amino acids was very high in contrast to partial editing at silent, third base, sites.
Kempken
F
F
Department of Plant Pathology, University of Florida, Gainesville 32611.
Mullen
J A
JA
Pring
D R
DR
Tang
H V
HV
D001483:Base Sequence; D003001:Cloning, Molecular; D004272:DNA, Mitochondrial; D002523:Edible Grain; D014644:Genetic Variation; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D006180:Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D004272:DNA, Mitochondrial; D006180:Proton-Translocating ATPases