A new human hypervariable locus (K29) maps to the q37.3 region of chromosome 2 and reveals a fingerprint.
A human genomic library was screened with a 30-base oligomer corresponding to the 5' end of the human calretinin cDNA. A clone that contains a minisatellite composed of 21 imperfect repeats of a 37-bp sequence was isolated. The consensus (GAGGGAGGAACTGGGACGCGTGCATGTTTGCATTCTC) incidentally shares 14 consecutive matches with the oligomer used as a probe, and it was shown that the clone did not belong to the calretinin locus. The minisatellite, named K29, was used as a probe on Southern blots at high stringency. After HaeIII, MboI, or HinfI digestion, it detected a single hypervariable locus, with 65% heterozygosity among Caucasian individuals. The probe used at low stringency revealed a fingerprint, with an average of four bands in addition to the locus-specific pattern. Mendelian inheritance was assessed on pedigrees. The K29 minisatellite was mapped by in situ hybridization to the very end of the long arm of chromosome 2 (2q37.3 band), at close proximity of the Fra2J locus, and is referred to as the D2S88 locus in the genome database.
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IRIBHN, Université libre de Bruxelles, Belgium.
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Mattei
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Vassart
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D001483:Base Sequence; D015139:Blotting, Southern; D064032:Calbindin 2; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D016384:Consensus Sequence; D016172:DNA Fingerprinting; D015342:DNA Probes / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D064030:S100 Calcium Binding Protein G / Q000235:genetics*; D044465:White People / Q000235:genetics
C576764:CALB2 protein, human; D064032:Calbindin 2; D015342:DNA Probes; D004276:DNA, Satellite; D009838:Oligodeoxyribonucleotides; D064030:S100 Calcium Binding Protein G