Enzymatic excision of radiation-induced lesions from DNA model compounds.
Dinucleoside monophosphates in which the 5' nucleoside contained a radiation-modified base were tested as substrates to bovine spleen phosphodiesterase (SPD) and snake venom phosphodiesterase. The radiation-modified bases included thymine glycols, 5-hydroxymethyluracil, 8-hydroxyguanine, and a formamido remnant of thymine. The lesions had widely different effects on diesterase action, varying from little inhibition, as in the case of digestion of dT*pA by SPD, where T* is the hydroxymethyluracil modification, to severe inhibition, as in the case of digestion of dG*pC by SPD, where G* is the 8-hydroxyguanine modification.
Maccubbin
A
A
Roswell Park Memorial Institute, Buffalo, New York 14263.
Evans
M
M
Paul
C R
CR
Budzinski
E E
EE
Przybyszewski
J
J
Box
H C
HC
D000818:Animals; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D005092:Exonucleases / Q000378:metabolism; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*
D004247:DNA; D005092:Exonucleases; C022650:spleen exonuclease; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I; C470790:phosphodiesterase I, snake venom