Induction of a cyclic AMP-responsive gene in living cells requires the nuclear factor CREB.
We constructed cell lines containing various enhancer elements cloned upstream from a marker gene. By microinjection of specific antibodies directed against transcriptional activator proteins into these cell lines, we have developed a functional assay for factors which regulate the activity of target promoters. Here we show that microinjection of a highly specific antibody to the cyclic AMP enhancer element-binding (CREB) protein diminishes gene expression in response to cyclic AMP in living fibroblasts.
Meinkoth
J L
JL
Cancer Center, University of California San Diego, La Jolla 92093.
Montminy
M R
MR
Fink
J S
JS
Feramisco
J R
JR
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000502:physiology*; D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins / Q000502:physiology*; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D007158:Immunologic Techniques; D008845:Microinjections; D051381:Rats
D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins; D000242:Cyclic AMP