title: Parathyroid hormone binding to cultured avian osteoclasts.
abstract: Parathyroid hormone (PTH) increases serum calcium concentration via a controversial cellular mechanism. We investigated whether PTH binds avian osteoclasts. Isolated hypocalcaemic hen osteoclasts were incubated with [125I]--bovine PTH (1-84). Specific binding of the hormone to the cells, which reached the equilibrium within 60 min, was observed. Half maximal binding was reached by 10 min. Binding was competitively inhibited by increasing doses of unlabeled PTH, and was about 55% displaced by adding, at the equilibrium, 10(-6) M unlabeled PTH. Autoradiography demonstrated specific label on the osteoclast. The cellular mechanism activated by the hormone remains to be elucidated.
authors.affiliation: Institute of Human Anatomy, University of Bari, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Teti
authors.forename: R
authors.initials: R
authors.lastname: Rizzoli
authors.forename: A
authors.initials: A
authors.lastname: Zambonin Zallone
mesh_terms: D000818:Animals; D001345:Autoradiography; D001667:Binding, Competitive; D002478:Cells, Cultured; D002645:Chickens; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D010010:Osteoclasts / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018016:Receptors, Parathyroid Hormone
chemical_list: D007457:Iodine Radioisotopes; D010281:Parathyroid Hormone; D011956:Receptors, Cell Surface; D018016:Receptors, Parathyroid Hormone