Differential changes of retina dopamine binding sites and adenylyl cyclase responses following 6-hydroxydopamine treatment.
Both dopamine D1 and D2 receptors are present in rat retina. D1 receptors are positively coupled to adenylyl cyclase, while D2 receptors are negatively coupled. After intraocular administration of the neurotoxin 6-hydroxydopamine (6-OHDA) there is depletion of retinal dopamine by about 90%, as well as a decrease of the number of D1 and D2 receptor binding sites. Following the 6-OHDA lesion, there is an enhancement of the D1 receptor-stimulated accumulation of cyclic-AMP and a loss of D2 receptor-inhibited accumulation of cyclic-AMP. Our results suggest that some of the retinal D2 receptors are coupled to adenylyl cyclase and some are not.
Hadjiconstantinou
M
M
Department of Psychiatry, Ohio State University College of Medicine, Columbus 43210.
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D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D000242:Cyclic AMP / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D006892:Hydroxydopamines / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D016627:Oxidopamine; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine / Q000187:drug effects / Q000378:metabolism*; D012160:Retina / Q000201:enzymology / Q000378:metabolism*
D006892:Hydroxydopamines; D011954:Receptors, Dopamine; D015102:3,4-Dihydroxyphenylacetic Acid; D016627:Oxidopamine; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D004298:Dopamine