Change in the expression of a nuclear matrix-associated protein is correlated with cellular transformation.
We have identified a monoclonal antibody that recognizes a nuclear matrix-associated protein in NIH 3T3 cells. The immunofluorescence pattern consists predominantly of bright nuclear granule clusters distributed throughout the nucleoplasm, with the exclusion of nucleoli. It recognizes a protein of 190 kDa that is down-regulated to various degrees in a panel of single-oncogene-transformed NIH 3T3 cells. Its localization is similar, but not identical, to the spliceosomal speckles. p190 shows a coordinate expression during the growth cycle of nontransformed NIH 3T3 cells; it is synthesized at the highest level under growth arrest conditions. It is expressed in adult mouse brain and is also present in human IMR-90 fibroblasts.
Brancolini
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International Center for Genetic Engineering and Biotechnology, Trieste, Italy.
Schneider
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D000818:Animals; D000911:Antibodies, Monoclonal; D034961:Antigens, Nuclear; D015153:Blotting, Western; D002453:Cell Cycle; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004261:DNA Replication; D005455:Fluorescent Antibody Technique; D006801:Humans; D051379:Mice; D008970:Molecular Weight; D015530:Nuclear Matrix / Q000648:ultrastructure*; D009687:Nuclear Proteins / Q000032:analysis* / Q000096:biosynthesis; D012342:RNA, Small Nuclear / Q000032:analysis; D012261:Ribonucleoproteins / Q000032:analysis; D017411:Ribonucleoproteins, Small Nuclear
D000911:Antibodies, Monoclonal; D034961:Antigens, Nuclear; D009687:Nuclear Proteins; D012342:RNA, Small Nuclear; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear