title: Branched saccharides formed by the action of His-modified cyclodextrin glycosyltransferase from Klebsiella pneumoniae M 5 al on starch.
abstract: Digestion of potato starch with His-modified alpha-cyclodextrin glycosyltransferase from Klebsiella pneumoniae M 5 al yielded branched tetra- to nona-saccharides, as revealed by debranching with pullulanase. Maltose and maltotriose stubs preponderated together with small proportions of D-glucose stubs. The branched saccharides accounted for approximately 1.2% of the starch.
authors.affiliation: Institut für Organische Chemie und Biochemie, Universität Freiburg i. Br., Germany.
authors.forename: H
authors.initials: H
authors.lastname: Bender
mesh_terms: D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D005964:Glucosyltransferases / Q000378:metabolism*; D006026:Glycoside Hydrolases; D006639:Histidine; D006868:Hydrolysis; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D011198:Solanum tuberosum; D013213:Starch / Q000737:chemistry* / Q000378:metabolism
chemical_list: D009844:Oligosaccharides; D006639:Histidine; D013213:Starch; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; D006026:Glycoside Hydrolases