title: Photoaffinity labeling of leukotriene binding sites in hepatocytes and hepatoma cells.
abstract: The method of direct photoaffinity labeling in the frozen state using the leukotrienes as suitable photolabile compounds may serve to identify and characterize polypeptides which interact with these eicosanoids during their hepatobiliary transport and metabolism. Furthermore, it will be a helpful technique to evaluate changes in the cell-specific protein pattern during neoplastic dedifferentiation of hepatocytes.
authors.affiliation: Deutsches Krebsforschungszentrum, Division of Tumor Biochemistry, Heidelberg, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Müller
authors.forename: E
authors.initials: E
authors.lastname: Falk
authors.forename: R
authors.initials: R
authors.lastname: Sandbrink
authors.forename: U
authors.initials: U
authors.lastname: Berger
authors.forename: I
authors.initials: I
authors.lastname: Leier
authors.forename: G
authors.initials: G
authors.lastname: Jedlitschky
authors.forename: M
authors.initials: M
authors.lastname: Huber
authors.forename: G
authors.initials: G
authors.lastname: Kurz
authors.forename: D
authors.initials: D
authors.lastname: Keppler
mesh_terms: D000345:Affinity Labels / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002352:Carrier Proteins / Q000378:metabolism*; D005615:Freezing; D006801:Humans; D015289:Leukotrienes / Q000378:metabolism* / Q000528:radiation effects; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008113:Liver Neoplasms / Q000473:pathology; D009363:Neoplasm Proteins / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D010777:Photochemistry; D051381:Rats; D014407:Tumor Cells, Cultured / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D000345:Affinity Labels; D002352:Carrier Proteins; D015289:Leukotrienes; D009363:Neoplasm Proteins; D010455:Peptides