Molecular cloning of cDNA for the import precursor of human subunit B of H(+)-ATP synthase in mitochondria.
The nucleotide sequence of the import precursor of subunit b of human H(+)-ATP synthase has been determined from a recombinant cDNA clone isolated by screening a human kidney cDNA library with a cDNA for rat subunit b as a probe. The sequence was composed of 1,134 nucleotides including a coding region for the import precursor of subunit b and noncoding regions on the 5'- and 3'-sides. The import precursor of subunit b and its mature polypeptide deduced from the open reading frame were found to consist of 256 and 214 amino acid residues with molecular weights of 28,893 and 24,610, respectively. The presequence of 42 amino acids could be the import signal peptide for directing the protein into the mitochondrial matrix.
Higuti
T
T
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Tsurumi
C
C
Osaka
F
F
Kawamura
Y
Y
Tsujita
H
H
Yoshihara
Y
Y
Tani
I
I
Tanaka
K
K
Ichihara
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004792:Enzyme Precursors / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D007668:Kidney / Q000201:enzymology; D046911:Macromolecular Substances; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000235:genetics; D006180:Proton-Translocating ATPases / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D004792:Enzyme Precursors; D046911:Macromolecular Substances; D021382:Protein Sorting Signals; D004247:DNA; D006180:Proton-Translocating ATPases