title: [Dose-dependence of the carboxymethylcellulose-papain combination in the prevention of peritoneal adhesions. Study in rats].
abstract: The action of a low volume (one drop) of sodium carboxymethylcellulose (CMC)-papain (PP) association gel to prevent peritoneal adhesions were studied in female Wistar rats. After ether anesthesia and a midline laparotomy incision, the right parietal peritoneum was pinched with a fine hemostat and the pinched peritoneal fold was then ligated. This maneuver was repeated thrice creating four points as if they were little "polyps" with a standardized size. Before closing the incision it was deposited on each point 0.05 ml (one drop) of CMC 2% (group A) or CMC + PP 0.4% (group B), with a total volume of 0.2 ml. These groups were compared with another similar group (group C) of a previous research, in which was used 7 ml/kg of body weight of CMC + PP 0.4% (1.5 ml by animal). Statistically significance was not noticed between groups A and B but it was noticed between these two groups (A and B) and group C (p < 0.001 and p < 0.01, respectively). It was concluded that the lowest effective volume (between 0.2 and 1.5 ml/animal) which can allow the desired effect is to be determined in order to diminish the quantity of substance to be deposited in the abdominal cavity.
authors.affiliation: Clínica Cirúrgica pela Faculdade de Medicina, Universidade de São Paulo.
authors.forename: O M
authors.initials: OM
authors.lastname: Parra
authors.forename: W A
authors.initials: WA
authors.lastname: Saad
authors.forename: S
authors.initials: S
authors.lastname: Ferri
authors.forename: L
authors.initials: L
authors.lastname: Peduto
authors.forename: J B
authors.initials: JB
authors.lastname: Ferraz-Neto
authors.forename: G M
authors.initials: GM
authors.lastname: Dal Colletto
mesh_terms: D000818:Animals; D002266:Carboxymethylcellulose Sodium / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004359:Drug Therapy, Combination; D005260:Female; D007813:Laparotomy; D010206:Papain / Q000008:administration & dosage*; D010532:Peritoneal Diseases / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D011919:Rats, Inbred Strains; D000267:Tissue Adhesions / Q000473:pathology / Q000517:prevention & control
chemical_list: D010206:Papain; D002266:Carboxymethylcellulose Sodium
vernacular_title: Dose-dependência da associação carboximetilcelulose-papaína na prevenção de aderências peritoneais. Estudo em ratos.