Autoradiographic mapping of 5-HT1, 5-HT1A, 5-HT1B and 5-HT2 receptors in the rat spinal cord.
The distribution of 5-HT1, 5-HT1A, 5-HT1B and 5-HT2 receptors in the rat spinal cord was investigated with quantitative autoradiography. Receptors were labeled respectively with [3H]serotonin (5-[3H]HT),8- hydroxy-2-[N-dipropylamino-3H]tetralin (8-OH-[3H]DPAT), [125I]iodocyanopindolol and [3H]ketanserin. It is shown that 5-HT1, 5-HT1A and 5-HT1B receptors are distributed within the spinal cord according to a rostro-caudal gradient. Both 5-HT1 and 5-HT1A receptors are mainly present in the dorsal horn and 5-HT1B is present throughout the spinal cord, exhibiting high densities in the caudal-most part of the dorsal horn in lamina X and in the sacral parasympathetic area. On the other hand, 5-HT2 receptors are shown mostly in the thoracic sympathetic area and in the thoracic ventral horn; the dorsal horn exhibits few 5-HT2 receptors. The differential involvement of 5-HT through different receptors in nociception, autonomous nervous system control and motility are discussed.
Marlier
L
L
INSERM U-336, Développement, Plasticité et Vieillissement du Système Nerveux, U.S.T.L., Montpeiller, France.
Teilhac
J R
JR
Cerruti
C
C
Privat
A
A
D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D000818:Animals; D001345:Autoradiography / Q000379:methods; D007457:Iodine Radioisotopes; D020120:Iodocyanopindolol; D007650:Ketanserin / Q000378:metabolism; D008297:Male; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011985:Receptors, Serotonin / Q000032:analysis* / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D012702:Serotonin Antagonists / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology* / Q000378:metabolism; D013764:Tetrahydronaphthalenes / Q000378:metabolism; D014316:Tritium
D007457:Iodine Radioisotopes; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; D013764:Tetrahydronaphthalenes; D014316:Tritium; D012701:Serotonin; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D020120:Iodocyanopindolol; D007650:Ketanserin; D010869:Pindolol