Methods compared for determining activity of N-acetyl-beta-D-glucosaminidase in urine without pretreatment of sample: different sensitivity and species effect.
N-Acetyl-beta-D-glucosaminidase (NAG) activities in the urine of men and rats were measured with methods recommended as procedures without pretreatment of the urine sample. Four different derivatives of NAG were compared for determination: 4-nitrophenyl; 3,3-dichlorophenylsulfonphthaleinyl; 3-cresolsulfonphthaleinyl, and 2-methoxy-4-(2-nitro-vinyl)phenyl. The conventional test using the 4-nitrophenyl derivative showed the highest activities and correlated very well with the other tests. There are method-dependent differences between NAG activities measured in men and rats due to the different Km values and inhibitory effects by urea.
Jung
K
K
Department of Experimental Organ Transplantation, University Hospital Charité, Humboldt University Berlin, FRG.
Priem
F
F
Klotzek
S
S
Becker
S
S
Henke
W
W
D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000378:metabolism*; D000118:Acetylglucosaminidase / Q000652:urine*; D000328:Adult; D000818:Animals; D006801:Humans; D007674:Kidney Diseases / Q000201:enzymology; D007700:Kinetics; D008297:Male; D010783:Photometry / Q000379:methods; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013379:Substrate Specificity
D000118:Acetylglucosaminidase; D000117:Acetylglucosamine