Nucleotide sequence of the DNA packaging and capsid synthesis genes of bacteriophage P2.
Overlapping DNA fragments containing the DNA packaging and capsid synthesis gene region of bacteriophage P2 were cloned and sequenced. In this report we present the complete nucleotide sequence of this 6550 bp region. Each of six open reading frames found in the interval was assigned to one of the essential genes (Q, P, O, N, M and L) by correlating genetic, physical and mutational data with DNA and protein sequence information. Polypeptides predicted were: a capsid completion protein, gpL; the major capsid precursor, gpN; the presumed capsid scaffolding protein; gpO; the ATPase and proposed endonuclease subunits of terminase, gpP and gpM, respectively; and a candidate for the portal protein, gpQ. These gene and protein sequences exhibited no homology to analogous genes or proteins of other bacteriophages. Expression of gene Q in E. coli from a plasmid caused production of a Mr 39,000 Da protein that restored Qam34 growth. This sequence analysis found only genes previously known from analysis of conditional-lethal mutations. No new capsid genes were found.
Linderoth
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NA
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Ziermann
R
R
Haggård-Ljungquist
E
E
Christie
G E
GE
Calendar
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R
D000251:Adenosine Triphosphatases / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics*; D004279:DNA, Viral / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D005816:Genetic Complementation Test; D005822:Genetic Vectors / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics
D036022:Capsid Proteins; D004279:DNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins; C079841:portal protein, bacteriophage P22; D004706:Endodeoxyribonucleases; C030894:terminase; D000251:Adenosine Triphosphatases