title: Mapping the receptor binding domain of interleukin-1 beta by means of binding studies using overlapping sequence fragments: why did it fail?
abstract: Interleukin-1 (IL-1) alpha and beta are polypeptide hormones that mediate a broad range of biological activities and interact with surface receptors on numerous cell types. Great efforts are made at present to define the interaction domain of IL-1 with its receptor. We have tried to map the domain of IL-1 beta by assessing the receptor interaction of synthetic octapeptide acid amides representing overlapping segments of the IL-1 beta primary sequence. Since the tertiary structure of IL-1 beta is known, the selection of octapeptides could be confined to the surface exposed residues. More than a 100 octapeptides were tested for binding in a competitive binding assay, using a mouse thymoma cell line (EL 4.61) as a receptor source and 125I-IL-1 alpha and beta as radioligands. No binding was found at up to a one hundred fold excess of octapeptide over radioligand. From this lack of binding we conclude that the entropic cost of conformationally freezing the octapeptide is high and that the conformation of the binding domain is per se in terms of free energy and is stabilized by the overall tertiary structure of IL-1.
authors.affiliation: Pharmaceuticals Division, CIBA-GEIGY Ltd., Basel, Switzerland.
authors.forename: U R
authors.initials: UR
authors.lastname: Joss
authors.forename: I
authors.initials: I
authors.lastname: Schmidli
authors.forename: K
authors.initials: K
authors.lastname: Vosbeck
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D007375:Interleukin-1 / Q000378:metabolism*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000737:chemistry*; D017472:Receptors, Interleukin-1; D014407:Tumor Cells, Cultured
chemical_list: D007375:Interleukin-1; D010446:Peptide Fragments; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1