The inhibition of restriction endonucleases due to Z-DNA in negatively supercoiled plasmid.
Plasmid pGC20 containing the (dGC)9 insert in SmaI recognition site has been used to study the inhibition of cleavage by different restriction endonuclease due to Z-DNA formation in (dCG)10 sequence of the negatively supercoiled plasmid. Data obtained indicate the different sensitivity of restriction endonucleases to DNA conformational perturbations resulted from the Z-DNA formation. Therefore, the inhibition of DNA cleavage by a particular restriction endonuclease cannot serve as a criterion for the estimation of the length of B-Z junctions in circular supercoiled DNAs.
Lesnik
E A
EA
Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Bes'chetnikova
Zh A
ZhA
Maslova
R N
RN
Varshavsky JaM
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D004262:DNA Restriction Enzymes / Q000037:antagonists & inhibitors*; D004278:DNA, Superhelical / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D010957:Plasmids
D004278:DNA, Superhelical; D004247:DNA; D004262:DNA Restriction Enzymes