title: ATP synthase complex from bovine heart mitochondria: the oligomycin sensitivity conferring protein is essential for dicyclohexyl carbodiimide-sensitive ATPase.
abstract: The requirement of bovine heart mitochondrial oligomycin sensitivity conferring protein (OSCP) in conferring dicyclohexylcarbodiimide (DCCD)-sensitivity to membrane-bound F1 was investigated by using OSCP-depleted membrane fraction (UF0) of ATP synthase. The ATPase activity of UF0-F1 was completely insensitive to DCCD while that of UF0-F1-OSCP was inhibited 95% by 16 microM DCCD. Both UF0-F1 and UF0-F1-OSCP complexes bound 5 nmol [14C]DCCD/mg UF0, and all the radioactivity was found to be associated with the DCCD-binding proteolipid. The data suggest that OSCP may be necessary for transmitting not only energy-linked signals, but also signals induced by F0 inhibitory ligands in mitochondrial energy transduction.
authors.affiliation: Department of Cell and Molecular Biology, Boston Biomedical Research institute 02114.
authors.forename: S
authors.initials: S
authors.lastname: Joshi
authors.forename: Y G
authors.initials: YG
authors.lastname: Huang
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D008929:Mitochondria, Heart / Q000187:drug effects / Q000201:enzymology*; D009840:Oligomycins / Q000494:pharmacology*; D006180:Proton-Translocating ATPases / Q000378:metabolism*
chemical_list: D009840:Oligomycins; D004024:Dicyclohexylcarbodiimide; D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases