FACS analysis of bovine leukemia virus (BLV)-infected cell lines with monoclonal antibodies (mAbs) to B cells and to monocytes/macrophages.
The eighteen monoclonal antibodies (mAbs) to B cells and the fourteen mAbs to accessory cells submitted to the workshop were analysed by FACS on three established, bovine leukemia virus (BLV)-infected bovine cell lines. Several mAbs of previously defined specificity were run in parallel. This analysis allowed us to gain further insight on the precise phenotype of those peculiar cells and to cluster the submitted mAbs according to their staining patterns. The BLV-infected cell lines seemed to belong to the B cell type though some of them lack detectable surface immunoglobulins. Moreover, all lines express the CD5 T cell marker and several myeloid markers.
Letesson
J J
JJ
Immunol. Dept., FNDP, Namur, Belgium.
Van den Broecke
A
A
Marbaix-Cleuter
Y
Y
Delcommenne
M
M
Mager
A
A
Mammerickx
M
M
Burny
A
A
Depelchin
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D002460:Cell Line; D002469:Cell Separation; D005434:Flow Cytometry; D016130:Immunophenotyping; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D008218:Lymphocytosis / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal