title: Transmission of bovine virus diarrhoea virus by blood feeding flies.
abstract: Three species of blood-feeding flies (Stomoxys calcitrans, Haematopota pluvialis and Hydrotaea irritans) were fed for five minutes on a bullock persistently infected with bovine virus diarrhoea virus (BVDV) containing 10(4.5)TCID50 non-cytopathic BVDV/ml serum, then subsequently fed on BVDV-free seronegative animals maintained in isolation. Virus was isolated from recipient animals between days 5 and 10 using H pluvialis, and up to 72 hours after transmission with S calcitrans; virus isolation was negative using H irritans. Positive seroconversion results obtained with H pluvialis gave a steadily increasing antibody titre. BVDV was recovered from flies 96 hours (four days) after an infective feed for H pluvialis and S calcitrans, but for two hours only for H irritans.
authors.affiliation: Central Veterinary Laboratory Weybridge, Surrey.
authors.forename: D W
authors.initials: DW
authors.lastname: Tarry
authors.forename: L
authors.initials: L
authors.lastname: Bernal
authors.forename: S
authors.initials: S
authors.lastname: Edwards
mesh_terms: D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000635:transmission*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000302:isolation & purification*; D004175:Diptera / Q000382:microbiology*; D005260:Female; D007303:Insect Vectors / Q000382:microbiology*; D008297:Male; D009117:Muscidae / Q000382:microbiology*; D012756:Sheep