Genome organization of membrane-containing bacteriophage PRD1.
We have determined the nucleotide sequence of the late region (11 kbp) of the lipid-containing bacteriophage PRD1. Gene localization was carried out by complementing nonsense phage mutants with genomic clones containing specific reading frames. The localization was confirmed by sequencing the N-termini of isolated gene products as well as sequencing the N-termini of tryptic fragments of the phage membrane-associated proteins. This, with the previously obtained sequence of the early regions, allowed us to organize most of the phage genes in the phage genome.
Bamford
J K
JK
Department of Genetics, University of Helsinki, Finland.
Hänninen
A L
AL
Pakula
T M
TM
Ojala
P M
PM
Kalkkinen
N
N
Frilander
M
M
Bamford
D H
DH
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D005816:Genetic Complementation Test; D006090:Gram-Negative Bacteria; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D015183:Restriction Mapping; D014759:Viral Envelope Proteins / Q000235:genetics
D004279:DNA, Viral; D014759:Viral Envelope Proteins; C118469:assembly factor P17