Alpha-1-stimulated phosphoinositide breakdown in cultured cardiomyocytes: diacylglycerol production and composition in docosahexaenoic acid supplemented cells.
The fatty acid pattern of phosphatidylinositol and other inositol phospholipids is reported to be predominantly 1-stearoyl, 2-arachidonyl. However, literature does not report data about the effect of a modification of this fatty acid composition on the production and acidic pattern of the diacylglycerol (DAG) formed during phosphoinositide hydrolysis. Culturing cardiomyocytes in a docosahexaenoic acid supplemented medium, we obtained an homogeneous cell population whose phospholipid fatty acid pattern was strongly different from control cells, and which produced, after alpha 1-adrenergic stimulation with phenylephrine, an higher amount of DAG. This DAG was different from control DAG in fatty acid composition, too. This structurally different DAG could be responsible for a different activation pattern of protein kinase C.
Bordoni
A
A
Dipartimento di Biochimica, University of Bologna, Italy.
Biagi
P L
PL
Rossi
C A
CA
Hrelia
S
S
D000818:Animals; D000831:Animals, Newborn; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002478:Cells, Cultured; D004075:Diglycerides / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis; D006352:Heart Ventricles / Q000187:drug effects / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism*; D010743:Phospholipids / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D001095:Arachidonic Acids; D004075:Diglycerides; D005227:Fatty Acids; D010716:Phosphatidylinositols; D010743:Phospholipids; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid