Distribution of dopamine D2 receptor mRNA splice variants in the rat by solution hybridization/protection assay.
We investigated the distribution of the two dopamine D2 receptor mRNA splice variants in the rat using a sensitive and quantitative solution hybridization/nuclease protection assay. In all brain and endocrine regions studied, both splice variants were detected and the mRNA of the longer form (D2L) was more abundant than that of the shorter form (D2S). The lowest percentages of D2S were found in the pituitary and adrenal glands.
Snyder
L A
LA
Fishberg Center for Neurobiology, Mount Sinai School of Medicine, New York, NY 10029.
Roberts
J L
JL
Sealfon
S C
SC
D000311:Adrenal Glands / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D008297:Male; D009693:Nucleic Acid Hybridization; D010902:Pituitary Gland / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine / Q000096:biosynthesis / Q000235:genetics*; D017448:Receptors, Dopamine D2
D012333:RNA, Messenger; D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D004247:DNA