title: N-terminal degradation of ACTH(4-10) and its synthetic analog semax by the rat blood enzymes.
abstract: Degradation of a regulatory peptide ACTH(4-10) and its synthetic analog semax in rat blood and serum was studied using high-performance liquid chromatography. About one third to one half of the serum degrading activity could be ascribed to bestatin-sensitive aminopeptidase which cleaved first and second N-terminal residues Met and Glu producing relatively stable intermediates. Comparable areas under the degradation/accumulation curves for intact peptides and intermediates implied that the latter can contribute to effects of intact peptides. Semax turned out to be more stable than ACTH(4-10) against the action of other enzymes that took part in degradation.
authors.affiliation: Institute of Molecular Genetics, USSR Academy of Sciences, Moscow.
authors.forename: V N
authors.initials: VN
authors.lastname: Potaman
authors.forename: L Y
authors.initials: LY
authors.lastname: Alfeeva
authors.forename: A A
authors.initials: AA
authors.lastname: Kamensky
authors.forename: N G
authors.initials: NG
authors.lastname: Levitzkaya
authors.forename: V N
authors.initials: VN
authors.lastname: Nezavibatko
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives* / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D007930:Leucine / Q000031:analogs & derivatives / Q000378:metabolism; D008297:Male; D010446:Peptide Fragments / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D010446:Peptide Fragments; C048487:ACTH (4-7), Pro-Gly-Pro-; D000324:Adrenocorticotropic Hormone; D007930:Leucine; C012211:ubenimex; C011394:ACTH (4-10)