Characterization of murine polyclonal antisera and monoclonal antibodies generated against intact and denatured human papillomavirus type 1 virions.
Human papillomavirus type 1 (HPV1) virions, both as intact virion particles (IVP) and as detergent-denatured virions (DDV), were used to prepare polyclonal antisera and monoclonal antibodies (MAbs) in BALB/c mice. Anti-IVP antiserum contained type-specific HPV1 L2-reactive antibodies and no detectable HPV1 L1-reactive antibodies. Anti-IVP MAbs recognized a linear epitope between L2 amino acids 102 and 108 (PIDVVDP). Anti-DDV antiserum contained type-specific HPV1 L1-reactive and HPV1 L2-reactive antibodies. An anti-DDV MAb recognized a linear epitope between L1 amino acids 127 and 133 (AENPTNY). HPV1a L1- and L2-encoded polypeptides expressed in Saccharomyces cerevisiae and by in vitro translation were equivalent in size to the major and minor virion capsid proteins, respectively.
Yaegashi
N
N
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Jenison
S A
SA
Valentine
J M
JM
Dunn
M
M
Taichman
L B
LB
Baker
D A
DA
Galloway
D A
DA
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004279:DNA, Viral / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005814:Genes, Viral; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D027383:Papillomaviridae / Q000235:genetics / Q000276:immunology*; D010455:Peptides / Q000276:immunology; D010957:Plasmids; D015183:Restriction Mapping; D014771:Virion / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D007106:Immune Sera; D010455:Peptides