Influence of aminophylline on the lipids in Microsporum gypseum.
The effect of aminophylline on the lipid synthesis of Microsporum gypseum has been examined. A decreased incorporation of [14C]acetate into lipids was observed when the cells were incubated for 1 h with aminophylline which was reflected in all the individual lipid fractions. However, cells grown with aminophylline in the growth medium exhibited increased levels of total phospholipids, which was probably due to a rise in intracellular cAMP as these cells exhibited 4-fold increased levels of cAMP. Decreased activity of phosphodiesterase by aminophylline accounts for the increased cAMP levels. Increased phospholipid content in aminophylline grown cells was further supported by the increased incorporation of [14C]acetate into phospholipids as well as increased activities of phospholipid biosynthetic enzymes in comparison to non-supplemented cells.
Bindra
A
A
Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Khuller
G K
GK
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D000085:Acetates / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism; D000628:Aminophylline / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D002797:Choline Kinase / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D005991:Glycerol Kinase / Q000378:metabolism; D050356:Lipid Metabolism; D008864:Microsporum / Q000187:drug effects / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis* / Q000302:isolation & purification; D010770:Phosphotransferases / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor); D011865:Radioisotope Dilution Technique
D000085:Acetates; D002250:Carbon Radioisotopes; D010743:Phospholipids; D000628:Aminophylline; D000242:Cyclic AMP; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); D005991:Glycerol Kinase; D002797:Choline Kinase; C020125:ethanolamine kinase; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D000262:Adenylyl Cyclases