2',3'-Dideoxycytidine preferentially inhibits in vitro growth of granulocyte-macrophage colony-forming cells from patients with chronic myeloid leukemia.
The effect of three nucleoside analogs, including 2-methyl-2'-deoxyadenosine (MDA), 5-amino-2'-deoxyuridine (ADU) and 2',3'-dideoxycytidine (DDC) on colony formation from unfractionated human bone marrow obtained from volunteers expressing parameters typical for normal cells (NBM) and from patients with the diagnosis of chronic myeloid leukemia (CMLBM) was observed. For the clonal growth of granulocyte-macrophage colony-forming cells (GM-CFC), a semisolid fibrin clot culture medium supplemented with 20% fetal bovine serum and 10% human placental conditioned medium was used. DDC has been shown to be at least a 10-fold more potent inhibitor for the growth of GM-CFC from CMLBM than from NBM. On the other hand, the effect of MDA and ADU on CMLBM did not differ markedly from the effect on NBM. These results suggest that DDC inhibits preferentially progenitor cells from CMLBM.
Cinatl
J
J
Department of Medical Virology, J.W. Goethe University, Frankfurt, FRG.
Cinatl
J
J
Gussetis
E S
ES
Nemeth
U
U
Mainke
M
M
Kornhuber
B
B
Brendel
M
M
Doerr
H W
HW
D001853:Bone Marrow / Q000187:drug effects* / Q000254:growth & development / Q000473:pathology; D003839:Deoxyadenosines / Q000494:pharmacology; D003857:Deoxyuridine / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000037:antagonists & inhibitors*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000187:drug effects; D016047:Zalcitabine / Q000494:pharmacology*
D003839:Deoxyadenosines; D016047:Zalcitabine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D003857:Deoxyuridine