Production of dimer-specific and dengue virus group cross-reactive mouse monoclonal antibodies to the dengue 2 virus non-structural glycoprotein NS1.
A panel of mouse monoclonal antibodies (MAbs) raised against the non-structural glycoprotein NS1 of dengue 2 virus (PR159) was studied for cross-reactivity with the NS1 protein of other dengue virus serotypes and other members of the Flaviviridae using immunoblotting. Most of the 35 anti-NS1 MAbs were found to be specific for dengue 2 virus NS1 (some of which were specific for the native, dimeric form of this protein), but others were found to cross-react within the dengue virus group. This latter group of MAbs, although dominated by MAbs defining a dengue 2 and 4 virus subgroup, also contained some MAbs that were shown to cross-react with both linear (sequential) and conformational epitopes common to the NS1 glycoproteins of all four dengue virus serotypes. Several of these MAbs were also able to cross-react with other flaviviruses, most notably viruses from the Japanese encephalitis antigenic complex. Although cross-reactive epitopes were previously demonstrated on this glycoprotein using polyclonal sera from dengue virus-infected animals and human, this is the first report of the isolation of MAbs which define these determinants and which will allow their further analysis.
Falconar
A K
AK
Wolfson Molecular Biology Unit, London School of Hygiene & Tropical Medicine, U.K.
Young
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PR
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000276:immunology; D002213:Capsid / Q000032:analysis / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions; D003716:Dengue Virus / Q000276:immunology*; D005416:Flavivirus / Q000276:immunology*; D015151:Immunoblotting; D046911:Macromolecular Substances; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D014709:Vero Cells; D014758:Viral Core Proteins / Q000032:analysis / Q000276:immunology*; D017361:Viral Nonstructural Proteins
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D046911:Macromolecular Substances; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins