Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product.
Hepatocyte growth factor (HGF) is a plasminogen-like protein thought to be a humoral mediator of liver regeneration. A 145-kilodalton tyrosyl phosphoprotein observed in rapid response to HGF treatment of intact target cells was identified by immunoblot analysis as the beta subunit of the c-met proto-oncogene product, a membrane-spanning tyrosine kinase. Covalent cross-linking of 125I-labeled ligand to cellular proteins of appropriate size that were recognized by antibodies to c-met directly established the c-met product as the cell-surface receptor for HGF.
Bottaro
D P
DP
Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892.
Rubin
J S
JS
Faletto
D L
DL
Chan
A M
AM
Kmiecik
T E
TE
Vande Woude
G F
GF
Aaronson
S A
SA
D002460:Cell Line; D003432:Cross-Linking Reagents; D006133:Growth Substances / Q000378:metabolism* / Q000502:physiology; D017228:Hepatocyte Growth Factor; D006801:Humans; D008970:Molecular Weight; D010766:Phosphorylation; D011233:Precipitin Tests; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D019859:Proto-Oncogene Proteins c-met; D011956:Receptors, Cell Surface / Q000378:metabolism*
D003432:Cross-Linking Reagents; D006133:Growth Substances; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D011956:Receptors, Cell Surface; D017228:Hepatocyte Growth Factor; D011505:Protein-Tyrosine Kinases; D019859:Proto-Oncogene Proteins c-met