The role of yeast artificial chromosome clones in generating genome maps.
The cloning of large DNAs as yeast artificial chromosomes (YACs) holds the promise of greatly expanding the scope of physical genomic mapping. Recent improvements in YAC clone libraries and their screening, as well as in the analysis of YAC-cloned DNA, are beginning to fulfill the potential of this system.
Burke
D T
DT
Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, New Jersey 08544.
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular / Q000379:methods*; D016521:Electrophoresis, Gel, Pulsed-Field; D005822:Genetic Vectors; D016678:Genome; D015698:Genomic Library; D006801:Humans; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013104:Spheroplasts