Prohormone processing by yeast proteases.
Investigations of the precursors of alpha-pheromone and killer toxin in the yeast Saccharomyces cerevisiae have defined the genes coding (KEX1 and KEX2) for the proteases which are responsible for their processing. In addition to processing at pairs of basic residues it is evident that yeast can also process at monobasic sites. We present data on the Kex1p and Kex2p enzymes, their cellular localization, and their post-translational modification. In addition initial characterisation of the monobasic specific protease and the isolation of mutants defective in this activity are presented. The use of the yeast system as a model for the processing of mammalian prohormones is discussed.
Bourbonnais
Y
Y
Eukaryotic Genetics Group, National Research Council of Canada, Montréal, Que.
Germain
D
D
Latchinian-Sadek
L
L
Boileau
G
G
Thomas
D Y
DY
D000595:Amino Acid Sequence; D043424:Carboxypeptidase B; D002268:Carboxypeptidases / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D055646:Killer Factors, Yeast; D000072235:Mating Factor; D008969:Molecular Sequence Data; D009183:Mycotoxins / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D043484:Proprotein Convertases; D011498:Protein Precursors / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000378:metabolism; D013379:Substrate Specificity; D013381:Subtilisins
D005656:Fungal Proteins; D055646:Killer Factors, Yeast; D009183:Mycotoxins; D010455:Peptides; D011498:Protein Precursors; D029701:Saccharomyces cerevisiae Proteins; D000072235:Mating Factor; D002268:Carboxypeptidases; C078874:carboxypeptidase D; D043424:Carboxypeptidase B; D043484:Proprotein Convertases; D012697:Serine Endopeptidases; D013381:Subtilisins; C058751:KEX2 protein, S cerevisiae