title: Aldosterone biosynthesis induced by ACTH and angiotensin II in newborn rat adrenocortical cells transfected by c-EJ-Ha-ras oncogene.
abstract: Adrenocortical cells were obtained by fractionated trypsination of newborn rat adrenal glands and transfected with a plasmid containing the EJ/T24-Ha-ras oncogene. Isolation of adhesive cells led to a proliferative cell line with an overexpression of 21 kDa ras protein. These cells incubated with corticosterone or deoxycorticosterone as the precursor produced a high level of 18-hydroxycorticosterone and aldosterone as identified by gas chromatography- mass spectrometry. ACTH and angiotensin II increased the basal production of aldosterone nineteen-fold and six-fold respectively. Under ACTH stimulation the ratio between aldosterone and 18-hydroxycorticosterone production was 1:3. The transformation of corticosterone under angiotensin II stimulation yielded up to 41% of 18-hydroxycorticosterone (4.7 micrograms/mg of cell protein per 24h) and 4.4% of aldosterone (0.5 microgram/mg of cell protein per 24h) in a low potassium concentration medium (6 mmol/l). To our knowledge this is the first report of continuous proliferative adrenocortical cells producing aldosterone.
authors.affiliation: Laboratoire de Biochimie des Interactions Cellulaires, Université de Bourgogne, Dijon, France.
authors.forename: G
authors.initials: G
authors.lastname: Maume
authors.forename: A
authors.initials: A
authors.lastname: Filali-Ansary
authors.forename: E
authors.initials: E
authors.lastname: Giannini
authors.forename: Y
authors.initials: Y
authors.lastname: Hathout
authors.forename: M
authors.initials: M
authors.lastname: Fischbach
authors.forename: B F
authors.initials: BF
authors.lastname: Maume
mesh_terms: D015069:18-Hydroxycorticosterone / Q000378:metabolism; D000311:Adrenal Glands / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000450:Aldosterone / Q000096:biosynthesis*; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics*; D051381:Rats; D014162:Transfection
chemical_list: D000804:Angiotensin II; D000450:Aldosterone; D015069:18-Hydroxycorticosterone; D000324:Adrenocorticotropic Hormone; D016283:Proto-Oncogene Proteins p21(ras)