title: Enzymatic excision of radiation-induced lesions from DNA model compounds.
abstract: Dinucleoside monophosphates in which the 5' nucleoside contained a radiation-modified base were tested as substrates to bovine spleen phosphodiesterase (SPD) and snake venom phosphodiesterase. The radiation-modified bases included thymine glycols, 5-hydroxymethyluracil, 8-hydroxyguanine, and a formamido remnant of thymine. The lesions had widely different effects on diesterase action, varying from little inhibition, as in the case of digestion of dT*pA by SPD, where T* is the hydroxymethyluracil modification, to severe inhibition, as in the case of digestion of dG*pC by SPD, where G* is the 8-hydroxyguanine modification.
authors.affiliation: Roswell Park Memorial Institute, Buffalo, New York 14263.
authors.forename: A
authors.initials: A
authors.lastname: Maccubbin
authors.forename: M
authors.initials: M
authors.lastname: Evans
authors.forename: C R
authors.initials: CR
authors.lastname: Paul
authors.forename: E E
authors.initials: EE
authors.lastname: Budzinski
authors.forename: J
authors.initials: J
authors.lastname: Przybyszewski
authors.forename: H C
authors.initials: HC
authors.lastname: Box
mesh_terms: D000818:Animals; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D005092:Exonucleases / Q000378:metabolism; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*
chemical_list: D004247:DNA; D005092:Exonucleases; C022650:spleen exonuclease; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I; C470790:phosphodiesterase I, snake venom