Physiologic activation of T cells via the T cell receptor induces NF-kappa B.
The transcription factor NF-kappa B has been implicated in the mitogen-induced expression of several genes that are critical for the immunologic function of T cells such as those encoding IL-2 and the IL-2R alpha chain (IL-2R alpha). We show here that NF-kappa B is induced in T cells activated by Ag, anti-CD3 antibody, or allogeneic stimulation. The induction of NF-kappa B via the TCR was dependent on protein kinase C. IL-2, which also activates IL-2R alpha expression and proliferation in T cells, was not able to induce NF-kappa B. TCR-mediated induction of NF-kappa B suggests a central role for this factor in activated T cells and also provides a mechanism for activation of latent HIV provirus during the normal immune response.
Jamieson
C
C
Rosenstiel Center, Brandeis University, Waltham, MA 02254-9110.
McCaffrey
P G
PG
Rao
A
A
Sen
R
R
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology; D001483:Base Sequence; D017252:CD3 Complex; D002478:Cells, Cultured; D002999:Clone Cells; D015870:Gene Expression; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000502:physiology; D007519:Isoantigens / Q000276:immunology; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex; D051379:Mice; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000235:genetics*; D009841:Oligonucleotides / Q000378:metabolism; D011493:Protein Kinase C / Q000502:physiology; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D015375:Receptors, Interleukin-2 / Q000502:physiology; D015398:Signal Transduction; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000502:physiology*
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D007376:Interleukin-2; D007519:Isoantigens; D016328:NF-kappa B; D009841:Oligonucleotides; D011948:Receptors, Antigen, T-Cell; D015375:Receptors, Interleukin-2; D011493:Protein Kinase C