Molecular cloning of genes encoding major two subunits of a eubacterial V-type ATPase from Thermus thermophilus.
The atpAB genes which encode the alpha and beta subunits of membrane ATPase from a thermophilic eubacterium, Thermus thermophilus HB8, were cloned. The deduced amino-acid sequences of the alpha subunit (583 amino acids) and the beta subunit (478 amino acids) are only moderately similar to the alpha beta subunits of the F0F1-ATPases, while they are highly similar to the major two subunits of the V-type ATPases, a family of ATPases which have been so far found in eukaryotic endomembrane vacuolar vesicles and archaebacterial plasma membranes. Thus, T. thermophilus ATPase belongs to the V-type ATPase family, even though this bacterium is a eubacterium. The hypothesis that the differentiation of an ancestral ATPase into V-type and F0F1-ATPase occurred after the evolution of a primordial cell into archaebacteria and eubacteria should be modified accordingly.
Tsutsumi
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S
Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.
Denda
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Yokoyama
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K
Oshima
T
T
Date
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T
Yoshida
M
M
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D006180:Proton-Translocating ATPases / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D016944:Sulfolobus / Q000201:enzymology / Q000235:genetics; D016963:Thermus thermophilus / Q000201:enzymology / Q000235:genetics*; D025262:Vacuolar Proton-Translocating ATPases
D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D000251:Adenosine Triphosphatases; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases