title: Identification of a common viral integration region in Cas-Br-E murine leukemia virus-induced non-T-, non-B-cell lymphomas.
abstract: The Cas-Br-E murine leukemia virus is a nondefective retrovirus that induces non-T-, non-B-cell lymphomas in susceptible NIH/Swiss mice. By using a DNA probe derived from Cas-Br-E provirus-flanking sequences, we identified a DNA region, originally called Sic-1, rearranged in 16 of 24 tumors analyzed (67%). All proviruses were integrated in a DNA segment smaller than 100 bp and were in the same 5'-to-3' orientation. Ecotropic as well as mink cell focus-forming virus types were found integrated in that specific DNA region. On the basis of Southern blot analysis of somatic cell hybrids and progeny of an interspecies backcross, the Sic-1 region was localized on mouse chromosome 9 near the previously described proto-oncogenes or common viral integration sites: Ets-1, Cbl-2, Tpl-1, and Fli-1. Restriction map analysis shows that this region is identical to the Fli-1 locus identified in Friend murine leukemia virus-induced erythroleukemia cell lines and thus may contain sequences also responsible for the development of mouse non-T-, non-B-cell lymphomas.
authors.affiliation: Département des Sciences Biologiques, Université du Québec à Montréal, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Bergeron
authors.forename: L
authors.initials: L
authors.lastname: Poliquin
authors.forename: C A
authors.initials: CA
authors.lastname: Kozak
authors.forename: E
authors.initials: E
authors.lastname: Rassart
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D015321:Gene Rearrangement; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D009052:Leukemia Virus, Murine / Q000235:genetics* / Q000472:pathogenicity; D008223:Lymphoma / Q000235:genetics / Q000382:microbiology*; D051379:Mice; D008815:Mice, Inbred Strains; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D015183:Restriction Mapping
chemical_list: D015342:DNA Probes; D004273:DNA, Neoplasm; D004279:DNA, Viral; D007143:Immunoglobulin Heavy Chains; D011948:Receptors, Antigen, T-Cell