A Mycoplasma hyorhinis protein with sequence similarities to nucleotide-binding enzymes.
We have determined the nucleotide (nt) and deduced amino acid (aa) sequence of a unique 115-kDa Mycoplasma hyorhinis protein (P115) with an N-terminal region containing a highly conserved consensus sequence characteristics of nt-binding domains of several ATPase and GTPase enzymes. However, P115 lacked additional conserved features characteristic of some classes of nt-binding proteins. Based on the hydropathy profile of the deduced aa sequence, the absence of a leader peptide, its exclusive partitioning into the hydrophilic phase during Triton X-114 phase fractionation of M. hyorhinis, and immunofluorescence analysis indicating no surface-exposed domains, it was concluded that P115 is a cytoplasmic protein lacking intrinsic membrane interaction. M. hyorhinis P115 appears to be a species-specific protein, since it was not detected in any other mycoplasmal or bacterial species examined with specific antibody or genomic probes. Since genetic systems for direct mutational analysis are currently unavailable in this organism, sequence analysis provides critical information in establishing the possible function of this protein. Moreover, the nt sequence encoding P115 reported here supports a previously proposed model, based on synthesis of P115-related proteins in Escherichia coli, suggesting that multiple polypeptide products can be generated from mycoplasma genes by promiscuous translation initiation in this heterologous expression system.
Notarnicola
S M
SM
Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri-Columbia 65212.
McIntosh
M A
MA
Wise
K S
KS
D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003062:Codon; D016384:Consensus Sequence; D005455:Fluorescent Antibody Technique; D020558:GTP Phosphohydrolases / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005798:Genes, Bacterial; D015151:Immunoblotting; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D003062:Codon; C067484:P115 protein, Mycoplasma hyorhinis; D000251:Adenosine Triphosphatases; D020558:GTP Phosphohydrolases