Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity.
We have cloned a group of cDNAs representing mRNAs that are rapidly induced following adherence of human monocytes. One of the induced transcripts (MAD-3) encodes a protein of 317 amino acids with one domain containing five tandem repeats of the cdc10/ankyrin motif, which is 60% similar (46% identical) to the ankyrin repeat region of the precursor of NF-kappa B/KBF1 p50. The C-terminus has a putative protein kinase C phosphorylation site. In vitro translated MAD-3 protein was found to specifically inhibit the DNA-binding activity of the p50/p65 NF-kappa B complex but not that of the p50/p50 KBF1 factor or of other DNA-binding proteins. The MAD-3 cDNA encodes an I kappa B-like protein that is likely to be involved in regulation of transcriptional responses to NF-kappa B, including adhesion-dependent pathways of monocyte activation.
Haskill
S
S
Lineberger Comprehensive Cancer Center, Department of Obstetrics and Gynecology, University of North Carolina, Chapel Hill 27599.
Beg
A A
AA
Tompkins
S M
SM
Morris
J S
JS
Yurochko
A D
AD
Sampson-Johannes
A
A
Mondal
K
K
Ralph
P
P
Baldwin
A S
AS
D000595:Amino Acid Sequence; D017487:Ankyrins; D001483:Base Sequence; D001798:Blood Proteins / Q000502:physiology; D015152:Blotting, Northern; D002448:Cell Adhesion; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D015870:Gene Expression; D006801:Humans; D020672:I-kappa B Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000502:physiology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology / Q000502:physiology*; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics
D017487:Ankyrins; D001798:Blood Proteins; D004268:DNA-Binding Proteins; D020672:I-kappa B Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D012333:RNA, Messenger; D000072000:NF-KappaB Inhibitor alpha; D004247:DNA