Disulfide bonds in native and recombinant fish growth hormones.
Disulfide linkages were characterized for the first time in a fish growth hormone. Trypsin digestion of chum salmon growth hormone, followed by mass spectrometry established that Cys-49 is linked to Cys-161, while Cys-178 is linked to Cys-186. This is analogous to the big loop, little loop pattern found in human growth hormone. Ninety-three percent of the primary structure of a recombinant rainbow trout growth hormone whose cDNA codes for the same amino acid sequence as chum salmon growth hormone was confirmed by mass spectrometric peptide mapping.
Vestling
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Department of Chemistry and Biochemistry, University of Maryland Baltimore County 21228.
Murphy
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Fenselau
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Chen
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D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin / Q000737:chemistry; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism; D013006:Growth Hormone / Q000737:chemistry* / Q000302:isolation & purification; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010449:Peptide Mapping; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D012474:Salmon / Q000378:metabolism*; D013455:Sulfur / Q000737:chemistry*; D014337:Trout / Q000378:metabolism*; D014357:Trypsin / Q000737:chemistry
D011994:Recombinant Proteins; D013455:Sulfur; D013006:Growth Hormone; D002918:Chymotrypsin; D014357:Trypsin