[Utilization of aminoacids in the study of the growth of Paracoccidioides brasiliensis. Influence on dimorphism].
Fifteen Paracoccidioides brasiliensis strains, in the mycelial (M) and yeast like (Y), were cultivated in minimal medium (MM) and subcultivated to be adapted to the same medium supplemented with a pool of amino acid in solution (MMS). Each of the amino acids were studied separately of the solution to provide the auxological study. The prototrophism was demonstrated by nine strains in both M and Y forms, and the auxotrophism by the remaining strains. The heterologous results has not allowed us to draw an auxological characterization of the P. brasiliensis. As far as we could observe none of the amino acid studied in this piece of research can be considered of absolute importance for to the growth and the morphogenesis maintainance of the fungus. Morphological alterations were only verified in the prototroph strains, which suggest that there could have been adaptative metabolism activity due to the absence of organic nitrogen compounds in the minimal medium (MM).
Cano
M I
MI
Laboratório de Investigação Médica, Hospital das Clínicas da Faculdade de Médicina da Universidade de São Paulo, Brasil.
de Aguiar
M S
MS
D000596:Amino Acids / Q000378:metabolism*; D010228:Paracoccidioides / Q000254:growth & development* / Q000378:metabolism; D012727:Sex Characteristics; D013696:Temperature
D000596:Amino Acids
Utilização de aminoácidos no estudo do crescimento do Paracoccidioides brasiliensis. Influência sobre o dimorfismo.