[Formation of erythroid colonies of bone marrow cells and their sensitivity to erythropoietin (EP) (in vitro) in piglets during the early postnatal period].
Collected at the first hours (0, 6, 12, 24, 48) and days (3 and 5) of piglets life bone marrow was cultured (in vitro) during 48 and 72 hours in order to assess the efficiency of development and ability of isolated marrow cells to form erythroid colonies (CFU-E-Colony Forming Unit) and also to examine the erythroidal sensitivity to Ep addition. It was found that bone marrow of new born piglets shows high ability to form erythroid colonies (CFU-E), but low sensitivity to Ep (erythropoietin). The limited efficiency to form immature erythroid colonies following 12h as well as matured ones after 24h, has been also shown. Observed changes could be caused by the limited erythroidal cells sensitivity to Ep.
Przała
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F
Zakład Higieny i Profilaktyki, Akademia Rolniczo-Techniczna, Olsztynie.
Bakuła
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T
Gajecki
M
M
Zduńczyk
E
E
Skorska-Wyszyńska
E
E
D000818:Animals; D000831:Animals, Newborn; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D015672:Erythroid Precursor Cells / Q000187:drug effects* / Q000502:physiology; D004921:Erythropoietin / Q000494:pharmacology*; D012016:Reference Values; D013552:Swine
D004921:Erythropoietin
Tworzenie kolonii erytroidalnych szpiku kostnego oraz ich wrazliwość na egzogenna erytropoetyne (Ep) (in vitro) u prosiat we wczesnym okresie postnatalnym.