Dopaminergic regulation of dopamine release from PC12 cells via a pertussis toxin-sensitive G protein.
Regulation of dopamine (DA) release from PC12 cells was investigated. Apomorphine and quinpirole, a selective D2 agonist, significantly reduced K(+)-evoked DA release, and this reduction was reversed by haloperidol. Furthermore, spiroperidol, a selective D2 antagonist, and haloperidol, a nonselective DA antagonist, enhanced the K(+)-evoked DA release. Pertussis toxin treatment of the cells abolished the quinpirole-induced reduction of K(+)-evoked DA release. Also, the haloperidol-induced enhancement of K(+)-evoked DA release was not seen in pertussis toxin treated cells. These results, therefore, suggest the presence of D2 receptors on PC12 cells which result in the modulation of K(+)-evoked DA release via a pertussis toxin-sensitive G protein.
Courtney
N D
ND
Department of Pharmacology, St. Louis University School of Medicine, MO 63104.
Howlett
A C
AC
Westfall
T C
TC
D000310:Adrenal Gland Neoplasms; D000818:Animals; D001058:Apomorphine / Q000494:pharmacology*; D002460:Cell Line; D004298:Dopamine / Q000378:metabolism / Q000502:physiology*; D018492:Dopamine Antagonists; D004873:Ergolines / Q000494:pharmacology*; D019204:GTP-Binding Proteins / Q000502:physiology*; D006220:Haloperidol / Q000494:pharmacology; D009538:Nicotine / Q000494:pharmacology; D037342:Pertussis Toxin; D010673:Pheochromocytoma; D011188:Potassium / Q000494:pharmacology; D019257:Quinpirole; D051381:Rats; D011954:Receptors, Dopamine / Q000187:drug effects / Q000502:physiology; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D013134:Spiperone / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D018492:Dopamine Antagonists; D004873:Ergolines; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D010566:Virulence Factors, Bordetella; D019257:Quinpirole; D013134:Spiperone; D009538:Nicotine; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D006220:Haloperidol; D001058:Apomorphine; D011188:Potassium; D004298:Dopamine