The protein kinase C-independent human B cell proliferation induced via surface immunoglobulins is unaffected by CD45 monoclonal antibodies.
In the present study the effect of 72-5D3 monoclonal antibody (CD45) on the proliferation induced by cross-linking of surface immunoglobulins on untreated and 4 beta-phorbol 12-myristate 13-acetate-treated human dense B cells was studied. The 72-5D3 mAb inhibited the proliferation induced via surface immunoglobulins alone or plus soluble T cell factors without affecting the release of inositol phosphate metabolites. However, after prolonged incubation (24 h) with high doses (100 ng/ml) of 4 beta-phorbol 12-myristate 13-acetate or mezerein, but not with 4 beta-phorbol, the same inhibitory effect did not take place. Therefore, our data support the hypothesis that protein kinase C is necessary for the antiproliferative effect of the 72-5D3 monoclonal antibodies.
Ingles-Esteve
J
J
Servei d'Immunologia, Hospital Clínic i Provincial, Villarroel, Barcelona, Spain.
Lozano
F
F
Plana
M
M
Alberola-Ila
J
J
Places
L
L
Vives
J
J
D015703:Antigens, CD / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000502:physiology*; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D006649:Histocompatibility Antigens / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000096:biosynthesis; D017493:Leukocyte Common Antigens; D008213:Lymphocyte Activation / Q000276:immunology*; D011233:Precipitin Tests; D011493:Protein Kinase C / Q000502:physiology*; D011947:Receptors, Antigen, B-Cell / Q000187:drug effects / Q000502:physiology
D015703:Antigens, CD; D006649:Histocompatibility Antigens; D007295:Inositol Phosphates; D011947:Receptors, Antigen, B-Cell; D011493:Protein Kinase C; D017493:Leukocyte Common Antigens