Sexual difference and organ specificity of the effect of estradiol on carbonic anhydrase and Mg(2+)-HCO3(-)-ATPase activities isolated from duodenal mucosa and kidney cortex of male and female rats: preliminary study with crude enzyme samples.
Effects of the s.c. administration of various doses of estradiol propionate (E.P.; 25-500 micrograms/kg) on the activities of carbonic anhydrase (CA), Mg(2+)-dependent ATPase and Mg(2+)-dependent, HCO3(-)-stimulated ATPase (Mg(2+)-HCO3(-)-ATPase) in rat duodenal mucosa and kidney cortex, and on body weight, organ weight and serum concentrations of testosterone and estradiol-17 beta, were examined in adult male, female, testectomized and ovariectomized rats. In normal male rats, activities of cytosol CA and brush border Mg(2+)-HCO3(-)-ATPase in the kidney were increased in a dose-dependent manner and reached 1.6- and 2-fold of controls, respectively, after consecutive administration (daily for 7 days) of 500 micrograms E.P. with no changes in either enzyme activities in duodenal mucosa. The positive correlations (P less than 0.01) were observed by linear regression analysis between serum concentration of estradiol-17 beta and kidney cytosol CA or kidney brush border Mg(2+)-HCO3(-)-ATPase activities. In normal female rats, activities of cytosol CA and brush border Mg(2+)-HCO3(-)-ATPase in the duodenal mucosa, and brush border Mg(2+)-HCO3(-)-ATPase activity in the kidney were increased by E.P. administration (100 and 500 micrograms/kg, daily for 7 days), however, kidney cytosol CA activity did not change by any dosage. Behavior of a part of both enzymes to E.P. in testectomized rats was altered almost in the same way to that observed in normal female rats and vice versa in ovariectomized rats. Body weight was decreased, in general, by consecutive administration of E.P. in a dose-dependent manner, and kidney weight was increased by E.P. in both male and female rats.
Suzuki
S
S
Department of Pharmacology, Kanazawa Medical University, Ishikawa, Japan.
Takamura
S
S
Yoshida
J
J
Ozaki
N
N
Niwa
O
O
D000818:Animals; D001835:Body Weight; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D002256:Carbonic Anhydrases / Q000378:metabolism*; D004386:Duodenum / Q000201:enzymology*; D004958:Estradiol / Q000502:physiology*; D005260:Female; D007413:Intestinal Mucosa / Q000201:enzymology*; D007672:Kidney Cortex / Q000201:enzymology*; D008297:Male; D008871:Microvilli / Q000201:enzymology; D009919:Orchiectomy; D009929:Organ Size; D010052:Ovariectomy; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012727:Sex Characteristics; D013379:Substrate Specificity
D004958:Estradiol; D017301:Ca(2+) Mg(2+)-ATPase; C032666:magnesium-bicarbonate ATPase; D002256:Carbonic Anhydrases