Platelet-activating factor stimulates the release of atrial natriuretic factor from the rat heart.
Atrial natriuretic factor (alpha-ANF (99-126), ANF) is released from atrial cells following atrial distension, myocardial infarction and periods of ischaemic or tachyarrhythmias. In this report we demonstrate that platelet-activating factor (PAF) stimulates ANF release from the isolated perfused rat heart and following i.v. injection to conscious unrestrained rats. ANF release peaked at 145% above baseline following injection of 2.5 nmol PAF into the isolated heart while administration of 2 nmol in vivo produced a 135% increase in plasma ANF levels. The PAF receptor antagonist BN52021 (10 mumol/l) attenuated this stimulated release, with the results suggesting a role for PAF in ANF secretion following release from damaged myocardium or as a humoral factor originating from the kidney.
Rayner
T E
TE
Department of Medicine, Flinders Medical Centre, Bedford Park, South Australia.
Menadue
M F
MF
Oliver
J R
JR
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes; D046934:Ginkgolides; D006321:Heart / Q000187:drug effects*; D007783:Lactones / Q000494:pharmacology; D008297:Male; D009206:Myocardium / Q000378:metabolism; D010477:Perfusion; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical
D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D010972:Platelet Activating Factor; C472610:atrial natriuretic peptide, rat; D009320:Atrial Natriuretic Factor; C045856:ginkgolide B