title: Cleavage capability of water buffalo follicular oocytes classified by cumulus cells and fertilized in vitro.
abstract: Water buffalo (Murrah) oocytes were collected from ovaries obtained from the slaughter house. They were classified according to the character of the cumulus cells under a stereomicroscope, and cultured in 25 mM Hepes buffered Tissue Culture Medium-199 (TCM-199) supplemented with 5% estrous water buffalo serum in an atmosphere containing 5% CO2 in air at 39 degrees C. After 20-24 hr of in vitro maturation, the oocytes were fertilized using capacitated sperm obtained from 4 different bulls. For cleavage the oocytes were cultured at 39 degrees C in TCM-199 supplemented with 1% estrous water buffalo serum and in an atmosphere containing 5% CO2 in air. The good oocytes, with compact and dense cumulus cells cleaved significantly higher (p less than 0.01, 67.3%), than those of fair. partially naked oocytes with thin cumulus layers (27.5%, 25/91) or small remnants of cumulus cells and poor naked oocytes (3/100). A substantial variation cumulus layers (27.5% 25/91) or small remnants of cumulus cells and poor naked oocytes (3/100). A substantial variation in fertilization and developmental rates (16.0% to 43.8%) was observed among 4 different bulls. Late non-surgically into 14 buffalo recipients on day 6 or 7 of their estrous cycle. One recipient was diagnosed to be pregnant by rectal palpation on day 60 and confirmed to be so on day 90 post-estrus.
authors.affiliation: Faculty of Veterinary Science, Yamaguchi University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: S K
authors.initials: SK
authors.lastname: Singla
authors.forename: J
authors.initials: J
authors.lastname: Sujata
authors.forename: M L
authors.initials: ML
authors.lastname: Madan
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000196:embryology*; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D009028:Morula / Q000502:physiology* / Q000637:transplantation; D009865:Oocytes / Q000502:physiology*