The murine Mov-34 gene: full-length cDNA and genomic organization.
The Mov-34 mutation is a recessive embryonic lethal mutation caused by experimental introduction of a recombinant Moloney murine leukemia provirus into the mouse germline. We have cloned a full-length cDNA from the Mov-34 gene, the transcription unit disrupted by the proviral integration. This cDNA is predicted to encode a novel 321-amino acid, 36-kDa protein of unknown function. Overlapping phage lambda clones containing the entire Mov-34 gene have been isolated. The Mov-34 gene spans just over 8 kb and contains seven exons. The 5' flanking region of the Mov-34 gene contains neither "TATA" nor "CAAT" box sequences, and 5' end mapping by primer extension and ribonuclease protection reveal multiple transcription initiation sites.
Gridley
T
T
Department of Cell and Developmental Biology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110.
Jaenisch
R
R
Gendron-Maguire
M
M
D000595:Amino Acid Sequence; D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D005091:Exons / Q000235:genetics; D005804:Genes, Lethal / Q000235:genetics*; D005808:Genes, Recessive / Q000235:genetics*; D047908:Intracellular Signaling Peptides and Proteins; D007942:Leukemia, Experimental / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D011506:Proteins; D011533:Proviruses / Q000235:genetics; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins / Q000235:genetics*; D012191:Retroviridae Proteins / Q000235:genetics*; D012260:Ribonucleases / Q000378:metabolism; D016385:TATA Box / Q000235:genetics
D004274:DNA, Recombinant; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D012191:Retroviridae Proteins; C072621:Psmd7 protein, mouse; D004247:DNA; D012260:Ribonucleases