Rat DNA sequence associated with a complex form of DNA polymerase alpha in nonregenerating liver interacts with a ubiquitous transcription/replication factor Oct-1.
A ubiquitous mammalian transcription factor, Oct-1 (also known as OTF-1, NF-A1, OBP100, or NFIII), stimulates the initiation of replication of adenovirus DNA, and may also be involved in the activation of some chromosomal replication origins. If this is true, binding sites for Oct-1 should be present within regions responsible for the initiation of DNA replication. In this study such a binding site has been identified within a 340bp fragment that was originally isolated from a minor fraction of DNA associated with a complexed form of DNA polymerase alpha from nonregenerating rat liver, and which shows autonomous replication sequence activity in a transient transfection assay. Northern blot analysis was used to show that Oct-1 mRNA is induced in regenerating rat liver 6-14 h after hepatectomy.
Timchenko
N A
NA
B P Konstantinov St Petersburg Institute of Nuclear Physics, Russian Academy of Sciences, Leningrad Region.
Zhuchenko
O P
OP
Timchenko
L T
LT
Iguchi-Ariga
S M
SM
Ariga
H
H
Bozhkov
V M
VM
Tomilin
N V
NV
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004247:DNA / Q000378:metabolism*; D004257:DNA Polymerase II / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D006498:Hepatectomy; D051863:Host Cell Factor C1; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008115:Liver Regeneration; D008969:Molecular Sequence Data; D050811:Octamer Transcription Factor-1; D051381:Rats; D014157:Transcription Factors / Q000378:metabolism*
D004268:DNA-Binding Proteins; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; C494168:Pou2f1 protein, rat; D014157:Transcription Factors; D004247:DNA; D004257:DNA Polymerase II; D003850:Deoxyribonuclease I