Highly effective extraction of carcinoembryonic antigen with phosphatidylinositol-specific phospholipase C.
Carcinoembryonic antigen (CEA) was highly effectively extracted with phosphatidylinositol-specific phospholipase C (PI-PLC) from various source materials. The yield of CEA was, in some cases, 20-30 times more than that obtained by the usual method with perchloric acid. Addition of 0.1-0.2 units of PI-PLC to 1 g tumor tissue and incubation at 37 degrees C for 1 h with continuous shaking seem to be practically sufficient for CEA extraction. A simple purification procedure for CEA after extraction with PI-PLC is proposed. The immunochemical properties of CEA thus obtained were found to be identical to those of CEA obtained by the usual method.
Matsuoka
Y
Y
Department of Biochemistry, School of Medicine, Fukuoka University, Japan.
Matsuo
Y
Y
Okamoto
N
N
Kuroki
M
M
Kuroki
M
M
Ikehara
Y
Y
D000328:Adult; D002272:Carcinoembryonic Antigen / Q000302:isolation & purification*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D007202:Indicators and Reagents; D007231:Infant, Newborn; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases; D011863:Radioimmunoassay; D014407:Tumor Cells, Cultured
D002272:Carcinoembryonic Antigen; D007202:Indicators and Reagents; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase