The effect of F0 inhibitors on the Vibrio alginolyticus membrane ATPase.
The inhibition of membrane ATPase from the marine alkalotolerant bacterium Vibrio alginolyticus by DCCD, triphenyltin and venturicidin was studied. DCCD proved to be an irreversible inhibitor, while venturicidin and triphenyltin produced a reversible inhibitory effect. The DCCD-binding proteolipid was identified in the membrane preparations. The effect of the inhibitors on ATPase activity and ATP-dependent Na(+)-transport in V. alginolyticus subcellular vesicles is discussed.
Capozza
G
G
Institute of Medical Biochemistry and Chemistry, University of Bari, Italy.
Dmitriev OYu
Krasnoselskaya
I A
IA
Papa
S
S
Skulachev
V P
VP
D000255:Adenosine Triphosphate / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D004024:Dicyclohexylcarbodiimide / Q000493:pharmacokinetics*; D009947:Organotin Compounds / Q000493:pharmacokinetics*; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D014698:Venturicidins / Q000493:pharmacokinetics*; D014733:Vibrio / Q000187:drug effects / Q000201:enzymology*
D009947:Organotin Compounds; D014698:Venturicidins; D004024:Dicyclohexylcarbodiimide; D000255:Adenosine Triphosphate; C030665:triphenyltin; D012964:Sodium; D006180:Proton-Translocating ATPases