title: A pgk::hprt fusion as a selectable marker for targeting of genes in mouse embryonic stem cells: disruption of the T-cell receptor delta-chain-encoding gene.
abstract: We have constructed a hypoxanthine phosphoribosyl transferase-selectable marker (hprt) under the control of the phosphoglycerate kinase (pgk) promoter. This construct permits cell growth in hypoxanthine/aminopterin/thymidine media and confers 6-thioguanine sensitivity upon mouse Hprt- embryonic stem cells, allowing either positive or negative selection in gene-targeting experiments. We have successfully targeted the gene encoding the T-cell receptor delta-chain using the pgk::hprt fusion for counterselection.
authors.affiliation: Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam.
authors.forename: N
authors.initials: N
authors.lastname: van der Lugt
authors.forename: E R
authors.initials: ER
authors.lastname: Maandag
authors.forename: H
authors.initials: H
authors.lastname: te Riele
authors.forename: P W
authors.initials: PW
authors.lastname: Laird
authors.forename: A
authors.initials: A
authors.lastname: Berns
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D005819:Genetic Markers; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D051379:Mice; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D010957:Plasmids; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D013234:Stem Cells
chemical_list: D005819:Genetic Markers; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011993:Recombinant Fusion Proteins; D007041:Hypoxanthine Phosphoribosyltransferase; D010735:Phosphoglycerate Kinase