[Attempts to culturing triatominae in the laboratory using "in vitro" feeding].
In order to simplify breeding of triatominae in the laboratory, for performing xenodiagnosis and other biologic studies, we tried to feed the insects "in vitro" with citrated or defibrinated blood from commercially abated chicken. Two types of efficacy observations were carried out with Triatoma infestans: a) analysis of the chaining of successive nymphal stages, viability of satisfactory matching, fertile oviposition and adequate reproduction; b) assessment of viability and infectivity of Trypanosoma cruzi in the insects. As a conclusion, it became evident that, despite operational easiness, the objectives were not achieved, since the classical procedure used as the control, was always superior.
de Souza
H B
HB
Laboratório de Investigação Médica-Parasitologia, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, Brasil.
Amato Neto
V
V
Braz
L M
LM
Moreira
A A
AA
Takiguti
C K
CK
Campos
R
R
Matsubara
L
L
D000818:Animals; D002645:Chickens; D010856:Columbidae; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D009758:Nymph / Q000254:growth & development; D010274:Parasitology / Q000379:methods*; D014224:Triatoma / Q000254:growth & development; D014225:Triatominae / Q000254:growth & development*; D014349:Trypanosoma cruzi / Q000502:physiology
Tentativas de criação de triatomíneos em laboratório por meio de alimentação "in vitro".