Primary structure of the nuclear-encoded 29.9 kDa subunit of NADH: ubiquinone reductase from Neurospora crassa mitochondria.
We isolated and sequenced cDNA for the 29.9 kDa subunit of mitochondrial NADH: ubiquinone reductase (complex I) from a Neurospora crassa library in the lambda gt11 expression vector. The N-terminus of the mature protein was determined by Edman-degradation. The cDNA contains an open reading frame encoding a preprotein of 273 amino acids. The presequence of the transit protein essential for mitochondrial import is eight residues long. Northern-blot analysis shows, that the level of the corresponding mRNA is increased 3-fold if cells are grown in the presence of chloramphenicol.
Van der Pas
J C
JC
Institut für Biochemie, Universität Düsseldorf, F.R.G.
Röhlen
D A
DA
Weidner
U
U
Weiss
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D004271:DNA, Fungal; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D016660:NAD(P)H Dehydrogenase (Quinone); D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D011808:Quinone Reductases / Q000235:genetics*
D004271:DNA, Fungal; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases