Transmembrane currents in frog olfactory cilia.
We have measured transmembrane currents in intact single cilia from frog olfactory receptor neurons. A single cilium on a neuron was sucked into a patch pipette, and a high-resistance seal was formed near the base of the cilium. Action potentials could be induced by applying suction or a voltage ramp to the ciliary membrane. A transient current was seen in some cells on stimulation with odorants. After excision from the cell, most of the cilia showed increased conductance in a bath containing cAMP, indicating that the cytoplasmic face of the ciliary membrane was accessible to the bath. The estimated resistance of a single cilium was surprisingly low.
Kleene
S J
SJ
Department of Anatomy and Cell Biology, University of Cincinnati, Ohio 45267-0521.
Gesteland
R C
RC
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002923:Cilia / Q000502:physiology*; D000242:Cyclic AMP / Q000494:pharmacology; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D004553:Electric Conductivity; D004594:Electrophysiology; D008564:Membrane Potentials / Q000502:physiology; D008839:Microelectrodes; D009474:Neurons / Q000502:physiology*; D009812:Odorants; D009831:Olfactory Mucosa / Q000294:innervation*; D011894:Rana pipiens
D004533:Egtazic Acid; D003994:Bucladesine; D000242:Cyclic AMP; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid