title: Arrest of MCF-7 cell migration by laminin in vitro: possible mechanisms.
abstract: Laminin, a major basement membrane component, arrested the migration of MCF-7/AZ human breast adenocarcinoma cells that were not invasive in vitro. Migration of invasive MCF-7/6 cells was not affected by laminin. Both cell types expressed the 67 kD laminin receptor, at both mRNA and protein level, but did not express the alpha 6 subunit of the VLA-6 integrin-type laminin receptor. The presence of YIGSR peptides (100 micrograms/ml), reported to block the interaction between laminin and its 67 kD receptor, did not change the migratory response of MCF-7/AZ or MCF-7/6 cells when meeting laminin lanes. In addition, the migration of these cell types was not affected by the presence of 17-beta-estradiol (10(-6) M) or all-trans retinoic acid (10(-6) M), which were both reported to increase the number of 67 kD receptors. We could therefore not assign an involvement of the 67 kD receptors in migration of MCF-7 cells on laminin, nor did we find evidence that conditioned medium of MCF-7/6 cells contains factors that are able to initiate migration of MCF-7/AZ cells on laminin.
authors.affiliation: Department of Radiotherapy and Nuclear Medicine, University Hospital, Gent, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Coopman
authors.forename: B
authors.initials: B
authors.lastname: Verhasselt
authors.forename: M
authors.initials: M
authors.lastname: Bracke
authors.forename: G
authors.initials: G
authors.lastname: De Bruyne
authors.forename: V
authors.initials: V
authors.lastname: Castronovo
authors.forename: M
authors.initials: M
authors.lastname: Sobel
authors.forename: J M
authors.initials: JM
authors.lastname: Foidart
authors.forename: F
authors.initials: F
authors.lastname: Van Roy
authors.forename: M
authors.initials: M
authors.lastname: Mareel
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000503:physiopathology*; D001943:Breast Neoplasms / Q000737:chemistry / Q000503:physiopathology*; D002465:Cell Movement / Q000187:drug effects; D003470:Culture Media; D006801:Humans; D007797:Laminin / Q000494:pharmacology*; D011946:Receptors, Antigen / Q000032:analysis; D011971:Receptors, Immunologic / Q000032:analysis / Q000378:metabolism; D017474:Receptors, Laminin; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D003470:Culture Media; D007797:Laminin; D011946:Receptors, Antigen; D011971:Receptors, Immunologic; D017474:Receptors, Laminin