The NoH value in EPR spin trapping: a new parameter for the identification of 5,5-dimethyl-1-pyrroline-N-oxide spin adducts.
The ratio of the nitrogen to hydrogen hyperfine splittings (aN/aH) of spin adducts derived from the spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) has been found to be a useful parameter for adduct identification. For example, this parameter makes it possible to distinguish between the superoxide (aN/aH = 1.22-1.26) and peroxyl (aN/aH = 1.33-1.40) radical adducts of DMPO in aqueous solution. Since the aN to aH ratio corrects for minor differences in EPR spectrometer calibration, it is a more reproducible parameter than the aN and aH values themselves.
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Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
Chignell
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D003497:Cyclic N-Oxides / Q000032:analysis*; D016208:Databases, Factual; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006859:Hydrogen / Q000032:analysis; D009584:Nitrogen / Q000032:analysis; D013113:Spin Labels
D003497:Cyclic N-Oxides; D005609:Free Radicals; D013113:Spin Labels; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D006859:Hydrogen; D009584:Nitrogen