NGF-induced neurite regeneration is mediated by a ras-independent pathway in PC12 cells.
A rat pheochromocytoma (PC12) cell line (designated MMTV-M17-5) expressing a dominant inhibitory mutant Ha-ras (Ha-ras Asn 17) protein was used to study nerve growth factor (NGF) induced neurite regeneration. Expression of the mutant p21 completely blocked NGF stimulated process formation in these cells. In contrast, neurite outgrowth induced by NGF treatment of primed MMTV-M17-5 cells was not significantly affected by the presence of Ha-ras Asn 17 protein. These observations suggest that, while ras function is required for NGF induced neuronal differentiation of PC12 cells, it is not needed to mediate NGF stimulated neurite regeneration.
Szeberényi
J
J
Department of Biology, University Medical School of Pécs, Hungary.
D000818:Animals; D002999:Clone Cells / Q000187:drug effects / Q000502:physiology; D011905:Genes, ras; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics / Q000502:physiology; D051381:Rats
D009414:Nerve Growth Factors; D016283:Proto-Oncogene Proteins p21(ras)