Effects of retinoic acid and bromodeoxyuridine on human melanoma-associated antigen expression in small cell lung carcinoma cells.
The dispersed neuroendocrine system includes cells with different embryological derivations, sharing a common neuroendocrine (NE) program, as indicated by the expression of NE markers, some of which are shared antigenic determinants. We report here that the small cell lung carcinoma cells NCI-H69 express the two human melanoma-associated antigens (HMAA) NGA/LS62 an LS109. Incubation of NCI-H69 cells with maturational inducers, such as retinoic acid and bromodeoxyuridine (BrdU), upregulated the expression of both HMAA. Exposure to BrdU for 4 weeks induced the appearance of a different phenotype in subpopulations of NCI-H69 cells, which became epithelioid, substrate-adherent, grew in monolayer and continued to express NE-associated antigens in variable amount. The shift in phenotype was not reversible after BrdU withdrawal and was maintained for at least 6 months in continuous culture. The substrate adhesion of NCI-H69 cells was paralleled by a change in NGA glycosylation pattern, thus suggesting a possible functional role for NGA in cell substrate adhesion/recognition.
Feyles
V
V
Oncology Research Group, University of Calgary, Alta., Canada.
Sikora
L K
LK
McGarry
R C
RC
Jerry
L M
LM
D000951:Antigens, Neoplasm / Q000032:analysis*; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D018288:Carcinoma, Small Cell / Q000276:immunology* / Q000473:pathology; D002448:Cell Adhesion; D005434:Flow Cytometry; D006801:Humans; D008175:Lung Neoplasms / Q000276:immunology* / Q000473:pathology; D008545:Melanoma / Q000276:immunology*; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins / Q000032:analysis*; D011233:Precipitin Tests; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D014212:Tretinoin; D001973:Bromodeoxyuridine