Induction of helper T cells by pertussis toxin during in vivo priming to insulin.
We have studied the effect of pertussis toxin (PT) on in vivo priming of T lymphocytes to insulin. Mice were immunized with bovine insulin in complete Freund's adjuvant and antigen-specific DNA synthesis was measured in lymphoid cell suspensions from lymph nodes and spleens. Insulin-specific response was greatly enhanced both in spleen and lymph nodes if mice were given PT at the time of immunization. Mice given PT presented 3 times more cells in spleen and 4 times less in lymph nodes. However, the major antigen-specific response was still observed in lymph nodes. PT had a strong mitogenic effect in vitro on lymph node cells but a weak effect on spleen cells indicating that the adjuvant activity of PT involves other effects besides the mitogenic activity.
Grenier-Brosette
N
N
Laboratoire d'Immunologie cellulaire et moléculaire, INSERM U210, Faculté de Médecine (PASTEUR), Nice, France.
Bettetini
D
D
Fehlmann
M
M
Cousin
J L
JL
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D007114:Immunization; D007328:Insulin / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens / Q000494:pharmacology; D037342:Pertussis Toxin; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects* / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D007328:Insulin; D008934:Mitogens; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin