title: Mechanisms of inhibitions of DNA polymerase gamma by nucleotide analogues having anti-HIV activities.
abstract: Inhibition mechanisms of 5'-triphosphates of 3'-azido-3'-deoxythymidine (AZT-TP) and 3'-deoxythymidine (ddTTP) on extensively purified DNA polymerase gamma from bovine testes were examined by analysis of the products synthesized on singly primed M13mp18 single-stranded DNA or synthetic oligonucleotide template-primer in the presence of analogues. The results indicate that AZT-TP inhibits DNA polymerase gamma in competition with dTTP but is not incorporated into DNA, whereas ddTTP is incorporated into DNA and causes chain termination.
authors.affiliation: Laboratory of Cancer Cell Biology, Nagoya University School of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Izuta
authors.forename: M
authors.initials: M
authors.lastname: Saneyoshi
authors.forename: T
authors.initials: T
authors.lastname: Sakurai
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Kojima
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002417:Cattle; D004258:DNA Polymerase III / Q000037:antagonists & inhibitors*; D054306:Dideoxynucleotides; D006678:HIV / Q000187:drug effects; D008297:Male; D013942:Thymine Nucleotides / Q000494:pharmacology*; D015215:Zidovudine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000998:Antiviral Agents; D054306:Dideoxynucleotides; D013942:Thymine Nucleotides; D015215:Zidovudine; C052873:zidovudine triphosphate; D004258:DNA Polymerase III; C017216:2',3'-dideoxythymidine triphosphate