title: Flow cytometric analysis of induced human graafian follicles. I. Demonstration and sorting of two luteinized cell populations.
abstract: OBJECTIVE
  To test the hypothesis that induced human graafian follicles consist of different steroidogenic cell types on the basis of their light scatter characteristics as determined by flow cytometry.
  
  
  METHODS
  Cross-sectional, observational study.
  
  
  METHODS
  Flow cytometry laboratory.
  
  
  METHODS
  Thirty-six follicular aspirates from nine consecutive patients undergoing in vitro fertilization for tubal factor infertility were evaluated.
  
  
  RESULTS
  Two distinct luteal cell populations were recovered. Both populations were positive by Oil Red O staining, suggesting the presence of intracellular lipid. Neither population stained positively for the presence of HLe-1/CD45, an antigen present on all human leukocytes.
  
  
  CONCLUSIONS
  Cellular heterogeneity exists within the granulosa cell compartment.
authors.affiliation: Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta 30912.
authors.forename: G F
authors.initials: GF
authors.lastname: Whitman
authors.forename: J P
authors.initials: JP
authors.lastname: Boldt
authors.forename: J E
authors.initials: JE
authors.lastname: Martinez
authors.forename: C G
authors.initials: CG
authors.lastname: Pantazis
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D002469:Cell Separation; D003430:Cross-Sectional Studies; D005260:Female; D005434:Flow Cytometry; D006649:Histocompatibility Antigens / Q000032:analysis; D006801:Humans; D017493:Leukocyte Common Antigens; D008027:Light; D008055:Lipids / Q000032:analysis; D006080:Ovarian Follicle / Q000737:chemistry / Q000166:cytology*; D012542:Scattering, Radiation
chemical_list: D015703:Antigens, CD; D006649:Histocompatibility Antigens; D008055:Lipids; D017493:Leukocyte Common Antigens