Ultraviolet light induction of lambda from dcm host strains alleviates EcoRII restriction of phage.
A new form of restriction alleviation is demonstrated for phage induced by ultraviolet light from dcm strains of Escherichia coli K-12. EcoRII restriction of the induced phage is alleviated, which is the first report of Type II restriction alleviation. Unlike previously reported restriction alleviation, the increase in phage-plating efficiency is not dependent upon irradiation of the plating host for its induction.
Radnedge
L
L
Department of Pharmaceutics, School of Pharmacy, University of London, U.K.
Pinney
R J
RJ
D010582:Bacteriophage lambda / Q000254:growth & development*; D015257:DNA-Cytosine Methylases / Q000096:biosynthesis*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D004790:Enzyme Induction / Q000528:radiation effects; D004926:Escherichia coli / Q000201:enzymology* / Q000528:radiation effects; D008242:Lysogeny; D014466:Ultraviolet Rays; D014775:Virus Activation / Q000528:radiation effects*
D015257:DNA-Cytosine Methylases; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific