[Laminin distribution in the clonal cells of PLA-801 (pulmonary giant cell carcinoma) cell line and its effect on tumor cell migration].
LN distribution in clone C and clone D cells of the PLA-801 (pulmonary giant cell carcinoma) cell line was studied with immunocytochemical staining. There were less LN and LN receptors found in clone C cells, which were from a low metastatic cell line, but much more LN and LNR were obtained in the highly metastatic clone D cells. The in vitro migration rates of these 2 clones were measured with agarose migratory analysis. The rate of clone D was much greater than that of clone C. With the presence of exgenous LN, clone C cells became activated with a higher migration rate and the migration rate of clone D was greatly reduced when cells were pretreated with anti-LN antibody. These results indicated that LN and LNR may play a vital role in the migration and metastasis of tumor cells.
Shan
Y
Y
Department of Pathology, General Hospital of PLA.
D000818:Animals; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology*; D002465:Cell Movement; D002999:Clone Cells / Q000737:chemistry; D006801:Humans; D007797:Laminin / Q000378:metabolism* / Q000502:physiology; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D011946:Receptors, Antigen; D011971:Receptors, Immunologic / Q000032:analysis; D017474:Receptors, Laminin
D007797:Laminin; D011946:Receptors, Antigen; D011971:Receptors, Immunologic; D017474:Receptors, Laminin