title: A Salmonella typhimurium virulence protein is similar to a Yersinia enterocolitica invasion protein and a bacteriophage lambda outer membrane protein.
abstract: The phoP-phoQ-regulated pagC locus is essential for full virulence and survival within macrophages of Salmonella typhimurium. The protein product, DNA sequence, and transcript of pagC were determined. The pagC locus encodes a single 188-amino-acid membrane protein that is similar to the ail-encoded eucaryotic cell invasion protein of Yersinia enterocolitica and the lom-encoded protein of bacteriophage lambda. The similarity of PagC and Ail to Lom leads us to hypothesize that Lom is a virulence protein and that bacteriophage gene transfer and lysogeny could have led to the development of proteins essential to survival within macrophages and eucaryotic cell invasion.
authors.affiliation: Infectious Disease Unit, Massachusetts General Hospital, Harvard Medical School, Boston 02114.
authors.forename: W S
authors.initials: WS
authors.lastname: Pulkkinen
authors.forename: S I
authors.initials: SI
authors.lastname: Miller
mesh_terms: D018829:Adhesins, Bacterial; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D003360:Cosmids; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D005798:Genes, Bacterial; D007256:Information Systems; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000235:genetics* / Q000472:pathogenicity; D012689:Sequence Homology, Nucleic Acid; D014774:Virulence / Q000235:genetics; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D008565:Membrane Proteins; C053476:invasin, Yersinia; C066766:pagC protein, Salmonella typhimurium