Reciprocal hybrid joints demonstrate successive V-J rearrangements on the same chromosome in the human TCR gamma locus.
Novel variable (V)--joining (J) gene rearrangements are described in the human T cell receptor gamma locus, in which, on the one hand, the V3 variable gene is joined to the heptamer--nonamer recombination signals of the J1 segment and, on the other hand, the J1 segment is joined to the V3 recombination signals through head-to-head fusion. These recombination products, or hybrid joints, have been originated through an inversion of 47 kb DNA. Interestingly the inverted DNA stretch contains a normal V9-J9 rearrangement. These findings are the first direct demonstration that successive rearrangements occur, on the same chromosome, in the human T cell receptor gamma locus, and suggest that the chronology of the joining events plays a role in the ontogeny of T cells and their differentiation in gamma/delta + and alpha/beta + lineages.
Alexandre
D
D
Laboratoire d'Immunogénétique Moléculaire, LIGM, Université Montpellier II, Sciences et Techniques du Languedoc, France.
Chuchana
P
P
Roncarolo
M G
MG
Yssel
H
H
Spits
H
H
Lefranc
G
G
Lefranc
M P
MP
D000595:Amino Acid Sequence; D001483:Base Sequence; D007446:Chromosome Inversion; D002999:Clone Cells; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D015329:Gene Rearrangement, T-Lymphocyte / Q000235:genetics* / Q000276:immunology; D006801:Humans; D019426:Integrases; D008969:Molecular Sequence Data; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics*; D045522:Recombinases; D011995:Recombination, Genetic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D016511:Severe Combined Immunodeficiency / Q000235:genetics / Q000276:immunology
D016692:Receptors, Antigen, T-Cell, gamma-delta; D045522:Recombinases; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C115006:integron integrase IntI1