title: 'Neuron-specific' protein gene product 9.5 (PGP 9.5) is also expressed in glioma cell lines and its expression depends on cellular growth state.
abstract: Protein gene product 9.5 (PGP 9.5), which in the normal nervous system is restricted to certain neurons, has been detected in two glioma cell lines, rat C6 and human GL15, by immunoblotting and immunocytochemistry. Its expression in these cells depends on the cellular growth state, being maximal between the first and second post-plating day. Only a faint PGP 9.5 immunoreactivity can be observed in glioma cells after the eleventh post-plating day, i.e. about one week after confluency has been reached. The present results suggest that PGP 9.5 in cultured glial cells is maximally expressed during the growth phase and that the protein could play a role during brain development in glial cells, in reactive gliosis, or in tumorigenesis of the glial lineage.
authors.affiliation: Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Giambanco
authors.forename: R
authors.initials: R
authors.lastname: Bianchi
authors.forename: P
authors.initials: P
authors.lastname: Ceccarelli
authors.forename: G
authors.initials: G
authors.lastname: Pula
authors.forename: G
authors.initials: G
authors.lastname: Sorci
authors.forename: S
authors.initials: S
authors.lastname: Antonioli
authors.forename: V
authors.initials: V
authors.lastname: Bocchini
authors.forename: R
authors.initials: R
authors.lastname: Donato
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002455:Cell Division; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured; D043222:Ubiquitin Thiolesterase
chemical_list: D009479:Neuropeptides; D043222:Ubiquitin Thiolesterase