Tumor infiltrating lymphocytes: the effect of bacillus Calmette Guerin on helper/suppressor-T cell ratios of treated and untreated tumors.
Copenhagen X Fischer rats bearing single and bilateral Dunning R3327 AT-3 tumors were either treated or not treated at a single site with bacillus Calmette Guerin (BCG). One week later tumors were removed, tumor infiltrating lymphocytes (TIL's) isolated, and then characterized for total-T, helper-T and suppressor-T cell subsets utilizing monoclonal antibodies. The purpose was to determine the effect of BCG on TIL's in treated as well as untreated tumors. In summary: 1) BCG treatment significantly alters TIL distributions at both injected and noninjected sites; 2) a noninjected contralateral tumor compromises the effectiveness of BCG therapy at the suppressor T cell level; 3) contralateral tumors, whether inoculated or not, have similar TIL distributions.
Rubenstein
M
M
Department of Cellular Biology, Hektoen Institute for Medical Research, Chicago, IL 60612.
Muchnik
S
S
Chet
M
M
Shaw
M W
MW
McKiel
C F
CF
Guinan
P D
PD
D000818:Animals; D001500:BCG Vaccine / Q000627:therapeutic use*; D002452:Cell Count; D002460:Cell Line; D008297:Male; D009368:Neoplasm Transplantation; D011471:Prostatic Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D051381:Rats; D006377:T-Lymphocytes, Helper-Inducer / Q000473:pathology*; D050378:T-Lymphocytes, Regulatory / Q000473:pathology*
D001500:BCG Vaccine