Isolation of infectious bursal disease virus from turkeys with arthritic and respiratory symptoms in commercial farms in Quebec.
Infectious bursal disease viruses (IBDVs) were isolated from turkeys showing symptoms of arthritis and respiratory disease in commercial poultry farms in the province of Quebec, Canada. Synovial fluids collected from hock joints of arthritic birds and peripheral blood leukocytes obtained from the birds with respiratory problems were used for virus isolation in embryonated chicken eggs, and Vero and BGM-70 cell cultures. The infected cells were evaluated for the presence of IBDV by indirect immunofluorescence assay using monoclonal antibodies. The viruses were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of viral genome and by electron microscopy. Although one of these turkey isolates tested was neutralized by serotype 1-specific commercial chicken antisera, preliminary results indicated that there are antigenic differences between the Quebec isolate, IBDV QT-1, and the existing strains of IBDV belonging to serotype 1.
Reddy
S K
SK
Faculté de Médecine Vétérinaire, Université de Montréal, St. Hyacinthe, Quebec, Canada.
Silim
A
A
D000818:Animals; D001170:Arthritis, Infectious / Q000382:microbiology / Q000662:veterinary; D002460:Cell Line; D005260:Female; D005455:Fluorescent Antibody Technique; D007243:Infectious bursal disease virus / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009500:Neutralization Tests; D011201:Poultry Diseases / Q000382:microbiology*; D011792:Quebec; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D012088:Reoviridae Infections / Q000382:microbiology / Q000662:veterinary*; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000662:veterinary; D013582:Synovial Fluid / Q000382:microbiology; D014422:Turkeys / Q000382:microbiology*
D012330:RNA, Double-Stranded; D012367:RNA, Viral