Correction of antigen-specific T cell defects in aged murine gut-associated lymphoid tissues an immune intervention by combined adoptive transfer of an antigen-specific immunoregulatory CD4 T cell subset and interleukin 2 administration.
Gut-associated lymphoid tissues (GALT) from aged mice enterically immunized with Mycobacterium paratuberculosis protoplasmic antigen show hyperreactive humoral immune responses; this hyperresponsiveness can be corrected to a considerable extent, but not entirely, by systemic administration of interleukin 2 (IL 2) alone. The aim of the present study was to determine further whether the hyperreactivity in the antigen-specific humoral immune responses in aged GALT could be fully restored by adoptive transfer of in vitro expanded antigen-specific IL 2-dependent helper (CD4+VV-) T cells from GALT in conjunction with recombinant IL 2 administration. The results show that the age-associated hyperresponsiveness in gut mucosal antigen-specific humoral immune responses can be entirely corrected by adoptive transfer with the antigen-specific GALT T helper cells together with in vivo IL 2 administration. The mechanism of this restoration involves reversal of the decline in antigen-specific CD8+ suppressor T cell functions in aged GALT.
Kawanishi
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H
Gut Mucosal Molecular Immunity Laboratory, University of Medicine & Dentistry of New Jersey, Robert Wood Johnson Medical School, New Brunswick 08903-0019.
Ajitsu
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S
D000375:Aging; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D016827:CD8 Antigens / Q000032:analysis; D002478:Cells, Cultured; D007116:Immunization, Passive; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology; D008212:Lymphocyte Depletion; D008643:Mesentery / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology; D010581:Peyer's Patches / Q000166:cytology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000907:Antibodies, Bacterial; D016827:CD8 Antigens; D007376:Interleukin-2