NMR spectroscopic study of single-stranded DNA fragments of d(CGGCGAAAGCCG) and d(CGGCAAAAGCCG).
We have investigated the structures of two kinds of single-stranded DNA fragments, d(CGGCGAAAGCCG) and d(CGGCAAAAGCCG), by use of NMR spectroscopy. It was found that the former takes a hair-pin like structure stabilized by hydrogen bonding of G/C base pairs in the stem region, while the latter takes a rather extended helical structure. The stable conformation of the former DNA is considered to originate from the stability of the sequence-specific conformation of the loop region rather than the stem region.
Tanikawa
J
J
Graduate School of Integrated Science, Yokohama City University, Japan.
Nishimura
Y
Y
Hirao
I
I
Miura
K
K
D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation
D004277:DNA, Single-Stranded