Inhibitory effect of highly purified human platelet beta-thromboglobulin on in vitro human megakaryocyte colony formation.
The effect of highly purified human beta-thromboglobulin (beta TG), a glycoprotein present in platelet alpha granules, was tested on human bone marrow in vitro megakaryocyte (MK) colony formation. A concentration of 5 micrograms/ml of beta TG induced a 50% reduction in the number of MK colonies, and concentrations of 10 and 20 micrograms/ml completely inhibited MK growth. This inhibition was of importance for MKs because a higher concentration of beta TG (10 micrograms/ml) was needed to obtain a nonsignificant decrease in erythroblastic progenitors (erythroid burst-forming units, BFU-E), and no inhibition of granulocyte-macrophage colony-forming units (CFU-GM) was observed. beta TG acts mainly on maturation of MKs. These results indicate that beta TG could play a role in the physiological regulation of platelet production by MKs.
Abgrall
J F
JF
Department of Hematology, Augustin Morvan Hospital, Brest University School of Medicine, France.
Han
Z C
ZC
Sensebe
L
L
Le Gall
G
G
Guern
G
G
Renard
I
I
Briere
J
J
D001792:Blood Platelets / Q000737:chemistry*; D002455:Cell Division / Q000187:drug effects; D004920:Erythropoiesis / Q000187:drug effects; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology*; D013997:Time Factors; D001620:beta-Thromboglobulin / Q000302:isolation & purification / Q000494:pharmacology*
D001620:beta-Thromboglobulin