Vasopressin receptors in the brain, liver and kidney of rats following osmotic stimulation.
The binding site concentration (Bmax) and equilibrium dissociation constant (Kd) for [3H]-arginine vasopressin (AVP) binding sites were measured in limbic brain areas (septum, dorsal hippocampus, amygdala) and liver and kidney of control and osmotically stimulated male Wistar rats. Membrane binding was performed in these five areas 30, 60 and 180 min following intraperitoneal injection of hypertonic saline. This paradigm resulted in no significant change in binding characteristics in the septum, dorsal hippocampus, amygdala and liver from control treated rats. In contrast, the kidney Bmax was significantly reduced 60 min following osmotic stimulation, with no effect on affinity. These results also suggest that AVP receptors in the CNS are relatively resistant to regulatory effects of an acute AVP exposure.
Landgraf
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Department of Cell Biology and Regulation, Karl Marx University, Leipzig, F.R.G.
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Dorsa
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D000818:Animals; D001921:Brain / Q000378:metabolism*; D006982:Hypertonic Solutions / Q000494:pharmacology*; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D009994:Osmolar Concentration; D051381:Rats; D011919:Rats, Inbred Strains; D011945:Receptors, Angiotensin / Q000378:metabolism*; D017483:Receptors, Vasopressin; D012965:Sodium Chloride / Q000494:pharmacology*; D014667:Vasopressins / Q000097:blood / Q000378:metabolism*
D006982:Hypertonic Solutions; D011945:Receptors, Angiotensin; D017483:Receptors, Vasopressin; D014667:Vasopressins; D012965:Sodium Chloride