Characterization of antisense DNA derivatives having stereoisomeric linkages.
The isomer separation of oligonucleoside phosphoramidates (OPA) by RPLC was studied. All stereoisomers of OPA-tetramer (dATCG) modified with isopropylamine could be separated and they showed different CD spectra each other. OPA-decamer (dGGGCATCGTC) modified with 3-amino-1-propanol could be separated into five fractions. Each fraction was found to have different ability to form hybrids with its complementary oligonucleotide, indicating that it is possible to exclude stereoisomers which can hardly bind to target nucleic acids.
Murakami
A
A
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Uematsu
H
H
Tamura
Y
Y
Nagahara
S
S
Ide
H
H
Makino
K
K
D000577:Amides / Q000737:chemistry; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D016373:DNA, Antisense / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010756:Phosphoric Acids / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013696:Temperature
D000577:Amides; D016373:DNA, Antisense; D010756:Phosphoric Acids; C011067:phosphoramidic acid