title: Quantitative measurement of monoclonal antibody distribution and blood flow using positron emission tomography and 124iodine in patients with breast cancer.
abstract: The uptake and in vivo quantitation of monoclonal antibodies (MAbs) has been measured non-invasively using positron emission tomography (PET) and 124iodine in 9 patients with breast ductal carcinoma. Blood-flow measurements were also made using 15oxygen-labelled water and PET to evaluate antibody delivery; 7 patients were studied with HMFGI antibody and 2 patients with a non-specific antibody. Tumour uptake ranged from 2-7.7 x 10(-3)% of injected dose per gram of tissue. Values for normal tissues including liver, lung and bone were also obtained. In 2 out of 7 patients studied with the specific antibody, uptake was greater than that seen with the non-specific antibody. There was no correlation between antibody uptake and blood flow. This report exemplifies the potential of PET for the non-invasive and accurate quantitative assessment of targeted antibody which is a prerequisite to therapy.
authors.affiliation: ICRF Oncology Group, Hammersmith Hospital London, UK.
authors.forename: C B
authors.initials: CB
authors.lastname: Wilson
authors.forename: D E
authors.initials: DE
authors.lastname: Snook
authors.forename: B
authors.initials: B
authors.lastname: Dhokia
authors.forename: C V
authors.initials: CV
authors.lastname: Taylor
authors.forename: I A
authors.initials: IA
authors.lastname: Watson
authors.forename: A A
authors.initials: AA
authors.lastname: Lammertsma
authors.forename: R
authors.initials: R
authors.lastname: Lambrecht
authors.forename: J
authors.initials: J
authors.lastname: Waxman
authors.forename: T
authors.initials: T
authors.lastname: Jones
authors.forename: A A
authors.initials: AA
authors.lastname: Epenetos
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000097:blood / Q000378:metabolism*; D001943:Breast Neoplasms / Q000098:blood supply / Q000000981:diagnostic imaging / Q000378:metabolism*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000098:blood supply / Q000000981:diagnostic imaging / Q000378:metabolism*; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D008875:Middle Aged; D012039:Regional Blood Flow; D013997:Time Factors; D014018:Tissue Distribution; D014055:Tomography, Emission-Computed / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes