Novel minimum ribozymes with oxidoreduction activity: 5-hydroxyuridine, 8-hydroxyguanosine, and 8-hydroxyadenosine isolated from Torula yeast RNA.
Three nucleosides catalyzing the oxidoreduction of NADH and K3Fe(CN)6 were isolated from Torula yeast RNA and also obtained by a series of steps: SDS-phenol extraction, nuclease P1 digestion, alkaline phosphatase digestion, anion exchange chromatography, and HPLC on an ODS column. Their chemical structures were clearly determined as 5-hydroxyuridine, 8-hydroxyguanosine, and 8-hydroxyadenosine from the results of FAB-MS, 1H and 13C-NMR spectroscopies.
Yanagawa
H
H
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Ogawa
Y
Y
Ueno
M
M
D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D003454:Cryptococcus / Q000201:enzymology* / Q000235:genetics; D006151:Guanosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010084:Oxidation-Reduction; D016337:RNA, Catalytic / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D012331:RNA, Fungal / Q000737:chemistry* / Q000378:metabolism; D014529:Uridine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
D016337:RNA, Catalytic; D012331:RNA, Fungal; D006151:Guanosine; C036009:8-oxoadenosine; C046215:8-hydroxyguanosine; C010194:5-hydroxyuridine; D000241:Adenosine; D014529:Uridine