Atrial natriuretic factor in mammalian testis: immunological detection in spermatozoa.
Immunoreactive atrial natriuretic factor (ANF) was localized by immunochemistry and radioimmunoassay in mouse and rat testes. The analyses of acid extracts of testes by gel filtration and reverse phase high pressure liquid chromatography (HPLC) revealed the presence of a processed 31-residues peptide and the precursor form of 126-residues pro-ANF molecule corresponding to a molecular weights (Mr) of 3,300 and 18,000, respectively. The concentration of ANF in mice testis averaged 12 +/- 3 ng and in rat testis 8 +/- 2 ng per g of tissue. Specific immunochemical staining was localized in the spermatids and elongating spermatozoa of mammalian testis. The demonstration of immunoreactive ANF in testis and specific localization in spermatids reveals a new site at which ANF may be actively synthesized and regulate paracrine and/or autocrine function(s) during spermiogenesis, suggesting a broader spectrum of ANF action in addition to its known regulatory role in the control of blood pressure homeostasis.
Pandey
K N
KN
Department of Biochemistry and Molecular Biology, Medical College of Georgia School of Medicine, Augusta 30912.
Orgebin-Crist
M C
MC
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis* / Q000302:isolation & purification; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008970:Molecular Weight; D011498:Protein Precursors; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013094:Spermatozoa / Q000737:chemistry*; D013737:Testis / Q000737:chemistry*
D011498:Protein Precursors; D009320:Atrial Natriuretic Factor