Effect of cytochrome P450 arachidonate metabolites on ion transport in rabbit kidney loop of Henle.
In the medullary segment of the thick ascending limb of the loop of Henle (mTALH), arachidonic acid (AA) is metabolized by a cytochrome P450-dependent monooxygenase to products that affect ion transport. The linkage between changes in ion transport and AA metabolism in isolated cells of the mTALH was examined. AA produced a concentration-dependent inhibition of 86Rb uptake--an effect that was prevented by selective blockade of cytochrome P450 monooxygenases. Inhibition by cytochrome P450 blockade of the effect of AA on 86Rb uptake could be circumvented by addition of the principal products of AA metabolism in the mTALH.
Escalante
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B
Department of Pharmacology, New York Medical College, Valhalla 10595.
Erlij
D
D
Falck
J R
JR
McGiff
J C
JC
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism* / Q000502:physiology; D001692:Biological Transport / Q000502:physiology; D002352:Carrier Proteins / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005665:Furosemide / Q000494:pharmacology; D066298:In Vitro Techniques; D008138:Loop of Henle / Q000187:drug effects / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D011817:Rabbits; D012414:Rubidium Radioisotopes; D012964:Sodium / Q000378:metabolism*; D028021:Sodium-Potassium-Chloride Symporters; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D001095:Arachidonic Acids; D002352:Carrier Proteins; D012414:Rubidium Radioisotopes; D028021:Sodium-Potassium-Chloride Symporters; D016718:Arachidonic Acid; D010042:Ouabain; D005665:Furosemide; D003577:Cytochrome P-450 Enzyme System; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium