title: Essential differences in oncogene involvement between primary nodal and extranodal large cell lymphoma.
abstract: Large cell lymphomas (LCLs) are heterogeneous in morphology, clinical presentation, and behavior. We studied 52 de novo LCLs of B-cell type for rearrangements of the bcl-2 and c-myc oncogenes by Southern blot analysis and related these data to the primary site of presentation, stage, and cytomorphology. Thirteen tumors had comigrating rearrangements of JH and bcl-2, indicative of a t(14;18). Far more primary nodal lymphomas than extranodal lymphomas carried a t(14;18) (40% v less than 5%). Additionally, almost all lymphomas with a t(14;18) versus 41% of the tumors without a bcl-2 rearrangement presented with lymphadenopathy. c-myc rearrangements were seen in 35% of the extranodal lymphomas and 5% of the nodal lymphomas. No differences were observed in bone marrow involvement and staging according to Ann Arbor. bcl-2 rearrangements were found in 50% of the LCLs with cleaved nuclei, whereas c-myc rearrangements were relatively frequent (25%) in the noncleaved subtype. Our data support the hypothesis that primary nodal and extranodal lymphomas have a different genetic origin.
authors.affiliation: Department of Hematology, University Medical Center, Leiden, The Netherlands.
authors.forename: S
authors.initials: S
authors.lastname: Raghoebier
authors.forename: M H
authors.initials: MH
authors.lastname: Kramer
authors.forename: J H
authors.initials: JH
authors.lastname: van Krieken
authors.forename: D
authors.initials: D
authors.lastname: de Jong
authors.forename: J
authors.initials: J
authors.lastname: Limpens
authors.forename: J C
authors.initials: JC
authors.lastname: Kluin-Nelemans
authors.forename: G J
authors.initials: GJ
authors.lastname: van Ommen
authors.forename: P M
authors.initials: PM
authors.lastname: Kluin
mesh_terms: D015139:Blotting, Southern; D015321:Gene Rearrangement; D016259:Genes, myc; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology*; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000473:pathology; D008297:Male; D009367:Neoplasm Staging; D009857:Oncogenes; D009928:Organ Specificity