Acute administration of ethanol suppresses pentylenetetrazole-induced c-fos expression in rat brain.
The effect of acute ethanol administration on pentylenetetrazole-induced c-fos expression in rat brain was studied. Pentylenetetrazole induced the rapid and transient expression of c-fos mRNA in rat brain. Maximal induction at a dose of 30 mg/kg was detected within 30 min and persisted for 60 min. Thereafter c-fos gene expression decreased to control levels by 180 min. No increase in c-fos mRNA was evident at doses of pentylenetetrazole < or = 20 mg/kg, whereas maximal elevation was seen at 30 or 40 mg/kg. This action was inhibited by acute ethanol treatment (blood alcohol level > or = 100 mg/dl). Acute ethanol treatment alone had no effect on c-fos gene expression.
Le
F
F
Department of Biochemistry, University of Queensland, Australia.
Wilce
P A
PA
Cassady
I
I
Hume
D A
DA
Shanley
B C
BC
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D015342:DNA Probes; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D016762:Genes, fos / Q000187:drug effects*; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010433:Pentylenetetrazole / Q000037:antagonists & inhibitors / Q000633:toxicity*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar
D015342:DNA Probes; D016760:Proto-Oncogene Proteins c-fos; D000431:Ethanol; D010433:Pentylenetetrazole