Molecular cloning of phosphofructokinase 1 gene from a thermophilic bacterium, Thermus thermophilus.
Phosphofructokinase 1 (PFK1) from Thermus thermophilus differs from other bacterial PFKs in that it is regulated by effector-induced reversible tetramer-dimer conversion rather than conformational change alone. We have cloned its gene and the deduced amino acid sequence was compared with that of other PFKs. While almost all amino acid residues involved in substrate binding sites, which are assigned from crystal structures of other PFKs, are well conserved, some possibly important changes are found at subunit interfaces.
Xu
J
J
Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.
Seki
M
M
Denda
K
K
Yoshida
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D001411:Geobacillus stearothermophilus / Q000201:enzymology / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010732:Phosphofructokinase-1 / Q000235:genetics*; D011487:Protein Conformation; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013824:Thermus / Q000201:enzymology / Q000235:genetics*
D007527:Isoenzymes; D046911:Macromolecular Substances; D015345:Oligonucleotide Probes; D010732:Phosphofructokinase-1