Molecular cloning and sequencing of plasma membrane H(+)-ATPase gene from the salt-tolerant yeast Zygosaccharomyces rouxii.
A plasma membrane H(+)-ATPase gene from the salt-tolerant yeast Zygosaccharomyces rouxii was isolated by probing the genomic DNA library with a labeled DNA fragment derived from the Saccharomyces cerevisiae H(+)-ATPase gene (PMA1) and the nucleotide sequence of a cloned gene was determined. The gene encoded a polypeptide of molecular weight 100,060 that consisted of 920 amino acid residues. The deduced amino acid sequence was 83% homologous to that of S. cerevisiae H(+)-ATPase and was highly similar to the conserved sequences in the plasma membrane ATPase family comprising yeast plasma membrane H(+)-ATPases. The peptide motifs involved in the ATPase functions were found in the Z. rouxii H(+)-ATPase sequence. The result of Northern analysis indicated that the size of the transcript of the Z. rouxii H(+)-ATPase gene was the same as that of S. cerevisiae PMA1.
Watanabe
Y
Y
Department of Bioresources, Faculty of Agriculture, Ehime University.
Shiramizu
M
M
Tamai
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002462:Cell Membrane / Q000201:enzymology; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D008969:Molecular Sequence Data; D006180:Proton-Translocating ATPases / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics; D016415:Sequence Alignment; D015003:Yeasts / Q000201:enzymology*
D004271:DNA, Fungal; D012331:RNA, Fungal; D006180:Proton-Translocating ATPases