Staurosporine both activates and inhibits serine/threonine kinases in human platelets.
The effects of staurosporine on a selection of protein kinases were investigated with thrombin-stimulated and control human blood platelets. The results demonstrate that staurosporine (1 microM) can lead to activation of certain protein kinases in intact platelets and has a general inhibitory effect on the renaturable protein kinases in vitro. New candidates for protein kinases involved in signal transduction are identified.
Kocher
M
M
Theodor Kocher Institute, University of Berne, Switzerland.
Clemetson
K J
KJ
D000470:Alkaloids / Q000494:pharmacology*; D001792:Blood Platelets / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008970:Molecular Weight; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000097:blood* / Q000302:isolation & purification; D017346:Protein Serine-Threonine Kinases; D019311:Staurosporine; D013917:Thrombin / Q000494:pharmacology
D000470:Alkaloids; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D013917:Thrombin; D019311:Staurosporine