Identification of a cis-acting sequence required for germ line-specific splicing of the P element ORF2-ORF3 intron.
P element transposition in Drosophila melanogaster is limited to the germ line because the third intron (the ORF2-ORF3 intron) of the P element transcript is spliced only in germ line cells. We describe a systematic search for P element sequences that are required to regulate the splicing of the ORF2-ORF3 intron. We have identified three adjacent mutations that abolish the germ line specificity and allow splicing of this intron in all tissues. These mutations define a 20-base regulatory region located in the exon, 12 to 31 bases from the 5' splice site. Our data show that this cis-acting regulatory sequence is required to inhibit the splicing of the ORF2-ORF3 intron in somatic cells.
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Department of Biology, University of California, Los Angeles 90024.
Zollman
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Tseng
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JC
Laski
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FA
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004251:DNA Transposable Elements / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005854:Germ Cells / Q000502:physiology*; D007438:Introns; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000502:physiology; D009841:Oligonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012045:Regulatory Sequences, Nucleic Acid
D004251:DNA Transposable Elements; D009687:Nuclear Proteins; D009841:Oligonucleotides