title: Primary structure of the nuclear-encoded 29.9 kDa subunit of NADH: ubiquinone reductase from Neurospora crassa mitochondria.
abstract: We isolated and sequenced cDNA for the 29.9 kDa subunit of mitochondrial NADH: ubiquinone reductase (complex I) from a Neurospora crassa library in the lambda gt11 expression vector. The N-terminus of the mature protein was determined by Edman-degradation. The cDNA contains an open reading frame encoding a preprotein of 273 amino acids. The presequence of the transit protein essential for mitochondrial import is eight residues long. Northern-blot analysis shows, that the level of the corresponding mRNA is increased 3-fold if cells are grown in the presence of chloramphenicol.
authors.affiliation: Institut für Biochemie, Universität Düsseldorf, F.R.G.
authors.forename: J C
authors.initials: JC
authors.lastname: Van der Pas
authors.forename: D A
authors.initials: DA
authors.lastname: Röhlen
authors.forename: U
authors.initials: U
authors.lastname: Weidner
authors.forename: H
authors.initials: H
authors.lastname: Weiss
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D004271:DNA, Fungal; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D016660:NAD(P)H Dehydrogenase (Quinone); D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D011808:Quinone Reductases / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases