Detection of individual Peyer's patch T cells that produce interleukin-5 and interferon-gamma.
A method was developed to detect individual interferon-gamma (IFN-gamma)- and interleukin-5 (IL-5)-producing cells among freshly isolated T cell populations and long term lines of CD4+ Peyer's patch T cells using frozen semi-thin sections of paraformaldehyde fixed-T cells and immunofluorescence techniques. Using this method, individual CD4+ Peyer's patch T cells could be shown to produce IFN-gamma, characteristic of the T helper 1 (Th1) T cell type, IL-5, characteristic of the T helper 2 (Th2) T cell type, as well as both IFN-gamma and IL-5. These data support the notion that Th1 and Th2 cells derive from a common T cell precursor.
Schoenbeck
S
S
Department of Medicine, University of California, San Diego, La Jolla 92093.
McCaffery
J M
JM
De Grandpre
L Y
LY
Kagnoff
M F
MF
D000818:Animals; D007371:Interferon-gamma / Q000096:biosynthesis*; D015848:Interleukin-5 / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000276:immunology*; D051381:Rats; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000378:metabolism*
D015848:Interleukin-5; D007371:Interferon-gamma