title: Human B cells cannot be triggered to kill target cells through their Fc gamma RII or Fc epsilon RII receptors.
abstract: There has been some controversy as to whether or not B cells can kill target cells through their Fc receptors. To address this, we have examined the ability of human B cells from a variety of sources to lyse hybridoma cells with specificity for either the B cell Fc gamma RII or Fc epsilon RII using a reverse killing assay, as well as their ability to lyse opsonized chicken erythrocytes using a classic ADCC assay. Tonsil B cells, chronic lymphocytic leukemia B cells, and Epstein-Barr virus-induced B cells, even after preactivation with a cocktail of cytokines, all failed to lyse any of these targets. We conclude that Fc gamma RII and Fc epsilon RII on human B cells are not cytotoxic trigger molecules.
authors.affiliation: Department of Immunology, U.C.M.S.M., London, U.K.
authors.forename: L E
authors.initials: LE
authors.lastname: MacKenzie
authors.forename: D V
authors.initials: DV
authors.lastname: Erbe
authors.forename: K A
authors.initials: KA
authors.lastname: Pulford
authors.forename: P M
authors.initials: PM
authors.lastname: Lydyard
authors.forename: M W
authors.initials: MW
authors.lastname: Fanger
mesh_terms: D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D000943:Antigens, Differentiation / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002461:Cell Line, Transformed; D002645:Chickens; D016207:Cytokines / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D008213:Lymphocyte Activation; D011961:Receptors, Fc / Q000276:immunology*; D017455:Receptors, IgE; D017452:Receptors, IgG
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D000944:Antigens, Differentiation, B-Lymphocyte; D016207:Cytokines; D007074:Immunoglobulin G; D011961:Receptors, Fc; D017455:Receptors, IgE; D017452:Receptors, IgG; D007073:Immunoglobulin E