Effect of sera of brain tumor patients on cytokine-induced human myelogenous leukemic cell differentiation.
Human myelogenous leukemic cell lines (ML-1, U-937, THP-1) were induced to differentiate by treatment with various cytokines such as tumor necrosis factor (TNF), interferon-gamma (IFN-gamma) and interleukin-6. Serum of normal humans efficiently stimulated this cytokine-induced differentiation, whereas the stimulating effect of serum of brain tumor patients was much less. Both normal and patient sera, at effective concentrations, only slightly affected the binding of 125I-TNF to ML-1 cells, but any of these sera significantly inhibited the binding of 125I-IFN-gamma to U-937 cells or to THP-1 cells. These results suggest that the weak differentiation-stimulating effect of the serum of brain tumor patients cannot be explained simply by the modification of the binding of these cytokines to cellular receptors.
Ikeda
M
M
Department of Neurosurgery, Showa University Fujigaoka Hospital, Yokohama, Japan.
Sakagami
H
H
Fukushima
Y
Y
D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000097:blood*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D016207:Cytokines / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute; D016403:Lymphoma, Large B-Cell, Diffuse; D008297:Male; D008875:Middle Aged; D011956:Receptors, Cell Surface / Q000378:metabolism; D011971:Receptors, Immunologic / Q000378:metabolism; D017471:Receptors, Interferon; D019947:Receptors, Interleukin-6; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism / Q000494:pharmacology
D016207:Cytokines; D015850:Interleukin-6; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D019947:Receptors, Interleukin-6; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma