Catabolism of 3- and 4-hydroxyphenylacetic acid by Klebsiella pneumoniae.
Klebsiella pneumoniae catabolizes both 4-hydroxyphenylacetic acid and 3-hydroxyphenylacetic acid via meta-cleavage of 3,4-dihydroxyphenylacetic acid, ultimately yielding pyruvate and succinate. The organism can synthesize two hydroxylases catalysing 3,4-dihydroxyphenylacetic acid formation, which differ in substrate specificity, cofactor requirement, kinetics and regulation. Five enzymes sequentially involved in the catabolism of 3,4-dihydroxyphenylacetic acid are encoded on a 7 kbp fragment of the K. pneumoniae chromosome that has been isolated in a recombinant plasmid.
Martín
M
M
Departamento de Bioquímica y Biología Molecular IV, Universidad Complutense, Madrid, Spain.
Gibello
A
A
Fernández
J
J
Ferrer
E
E
Garrido-Pertierra
A
A
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D015394:Molecular Structure; D009154:Mutation; D010084:Oxidation-Reduction; D010648:Phenylacetates / Q000378:metabolism*; D010957:Plasmids; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid; D014169:Transformation, Bacterial
D010648:Phenylacetates; D011773:Pyruvates; D013386:Succinates; D015102:3,4-Dihydroxyphenylacetic Acid; C008070:4-hydroxyphenylacetic acid; D019289:Pyruvic Acid; D019802:Succinic Acid; D000242:Cyclic AMP; D006899:Mixed Function Oxygenases; C026253:3-hydroxyphenylacetate 4-monooxygenase; C026252:4-hydroxyphenylacetate 3-monooxygenase; C008069:3-hydroxybenzeneacetic acid