Enzymatic method for determination of the degree of deacetylation of chitosan.
A new method was developed for the accurate determination of the degree of deacetylation of chitosan. The method involves the complete hydrolysis of chitosan to glucosamine and N-acetylglucosamine by a cooperative action of chitosanolytic enzymes exo-beta-D-glucosaminidase, beta-N-acetylhexosaminidase, and chitosanase, and subsequent determination of the monosaccharides by specific colorimetric assays or HPLC. The conditions required for the complete hydrolysis of chitosan were examined and the degree of deacetylation of several chitosan samples was determined.
Nanjo
F
F
Research and Development Laboratory, Yaizu Suisan Kagaku Industry Co., Ltd., Shizuoka, Japan.
Katsumi
R
R
Sakai
K
K
D000107:Acetylation; D000117:Acetylglucosamine / Q000138:chemical synthesis*; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry; D048271:Chitosan; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D005944:Glucosamine / Q000138:chemical synthesis*; D006026:Glycoside Hydrolases / Q000032:analysis; D006596:Hexosaminidases / Q000032:analysis; D006868:Hydrolysis; D015203:Reproducibility of Results; D001619:beta-N-Acetylhexosaminidases / Q000032:analysis
D002686:Chitin; D048271:Chitosan; D006026:Glycoside Hydrolases; D006596:Hexosaminidases; C019868:chitosanase; D001619:beta-N-Acetylhexosaminidases; D005944:Glucosamine; D000117:Acetylglucosamine