In vitro regulation of B cell differentiation by interleukin-6 and soluble CD23 in systemic lupus erythematosus B cell subpopulations and antigen-induced normal B cells.
Polyreactive systemic lupus erythematosus (SLE) B cells were compared with antigen-induced SLE and normal B cells for their interleukin-6 (IL-6) and soluble CD23 requirements. Unlike normal B cells, secretion of antibody by SLE B cells in serum-free medium was not enhanced by exogenous IL-6. Anti-IL-6 antibodies inhibited immunoglobulin production in cultures of normal and SLE B cells, which suggests that IL-6 is required for B cell differentiation. SLE culture supernatants had elevated levels of IL-6, which explains the poor response of the SLE cells to exogenous IL-6. Soluble CD23 enhanced the responses of cells from normal subjects and SLE patients.
Klashman
D J
DJ
Department of Medicine, University of California, Los Angeles School of Medicine 90024.
Martin
R A
RA
Martínez-Maza
O
O
Stevens
R H
RH
D000328:Adult; D000917:Antibody Formation; D015703:Antigens, CD / Q000494:pharmacology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000494:pharmacology*; D016175:B-Lymphocyte Subsets / Q000473:pathology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000473:pathology*; D002454:Cell Differentiation; D005260:Female; D006801:Humans; D007114:Immunization; D015850:Interleukin-6 / Q000096:biosynthesis / Q000494:pharmacology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011961:Receptors, Fc / Q000494:pharmacology*; D017455:Receptors, IgE; D012995:Solubility; D013745:Tetanus Toxoid / Q000276:immunology*
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D015850:Interleukin-6; D011961:Receptors, Fc; D017455:Receptors, IgE; D013745:Tetanus Toxoid