Effects of antioxidant supplementation on platelet function: a randomized pair-matched, placebo-controlled, double-blind trial in men with low antioxidant status.
We investigated the effect on platelet function of supplementing men with low antioxidant status with 600 mg ascorbic acid, 300 mg alpha-tocopherol, 27 mg beta-carotene, and 75 micrograms selenium in yeast daily. Eighty men were randomly assigned in pairs (matched for smoking, baseline antioxidant status, and time and day of entry) by use of a double-blind design to receive supplement or placebo for 5 mo. Compared with 39 control subjects, 39 antioxidant-supplemented men experienced the following net reductions during the double-blind period: 20% (P = 0.012) in serum lipid peroxides, 24% (P = 0.035) in ADP-induced platelet aggregation, 42% (P = 0.040) in the rate of ATP release during aggregation, 51% (P = 0.018) in serum (platelet-produced) thromboxane B2, and 29% (P = 0.024) in plasma beta-thromboglobulin concentration. The data support our hypothesis that antioxidant supplementation of men with low antioxidant status and high fat intake reduces lipid peroxidation, the capacity of platelets to aggregate and to produce thromboxane A2, and in vivo platelet activation.
Salonen
J T
JT
Department of Community Health and General Practice, University of Kuopio, Finland.
Salonen
R
R
Seppänen
K
K
Rinta-Kiikka
S
S
Kuukka
M
M
Korpela
H
H
Alfthan
G
G
Kantola
M
M
Schalch
W
W
D000255:Adenosine Triphosphate / Q000378:metabolism; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects*; D002338:Carotenoids / Q000494:pharmacology; D004041:Dietary Fats / Q000008:administration & dosage; D004311:Double-Blind Method; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D008875:Middle Aged; D015539:Platelet Activation / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D012643:Selenium / Q000494:pharmacology; D012907:Smoking / Q000097:blood; D013929:Thromboxane B2 / Q000097:blood; D014810:Vitamin E / Q000494:pharmacology; D019207:beta Carotene; D001620:beta-Thromboglobulin / Q000032:analysis
D000975:Antioxidants; D004041:Dietary Fats; D008054:Lipid Peroxides; D010975:Platelet Aggregation Inhibitors; D001620:beta-Thromboglobulin; D019207:beta Carotene; D014810:Vitamin E; D002338:Carotenoids; D013929:Thromboxane B2; D000255:Adenosine Triphosphate; D012643:Selenium; D001205:Ascorbic Acid