Histological and biochemical characteristics of kryoprotected embryonic nervous tissue for transplantation purpose.
In clinical and experimental conditions, a biochemical equipment and a histological image of a fresh embryonic nervous tissue and a tissue conserved by deep freezing for transplantation purposes were examined. By repeated examination of neurotransmitters, the contents of dopamine and serotonin were found unchanged by freezing. In a human embryo, serotonin predominates over dopamine, while in chicken embryos, used for xeno-transplantations, it is vice versa. As for the microscopic structure of nervous tissue, no influence of kryopreservation for 4 to 14 days was found.
Nádvorník
P
P
Department of Experimental Neurology, Palacký University, Olomouc.
Rozhold
O
O
Kolarík
J
J
Ondra
P
P
Závodný
P
P
Hlustík
P
P
D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D016380:Brain Tissue Transplantation; D002642:Chick Embryo; D015925:Cryopreservation; D016332:Fetal Tissue Transplantation; D006801:Humans