Studies of in vitro megakaryocytopoiesis in adult immune thrombocytopenic purpura (ITP).
In vitro megakaryocytopoiesis was studied in 8 patients with chronic immune thrombocytopenia (ITP). A significant increase of megakaryocyte (MK) colony formation was observed in 5/5 patients studied. Furthermore, the serum of these 8 patients was able to enhance MK colony formation by normal marrow cells. This effect was neither due to a decrease of inhibitors of megakaryocytopoiesis such as betathromboglobulin (beta TG) nor to the IgG fraction of patients' serum. In addition, the level of interleukin 6, which is above all a stimulus for MK maturation, was found within the normal range in 8/8 patients tested. These data suggest that in chronic ITP there is an increase of MK progenitor cell number which may be due to an increased level of MK colony-stimulating activity.
Bellucci
S
S
Department of Angio-Hematology-IVS 2, Paris, France.
Han
Z C
ZC
Caen
J P
JP
D000328:Adult; D001792:Blood Platelets / Q000378:metabolism; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005260:Female; D006410:Hematopoiesis; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D066298:In Vitro Techniques; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008533:Megakaryocytes; D008875:Middle Aged; D011696:Purpura, Thrombocytopenic / Q000473:pathology; D001620:beta-Thromboglobulin / Q000096:biosynthesis
D007074:Immunoglobulin G; D015850:Interleukin-6; D001620:beta-Thromboglobulin