Analysis of Ly5 chromosome 1 position using allelic differences and recombinant inbred mice.
Differences between the mouse Ly5a and Ly5b alleles can be distinguished on the basis of polymerase chain reaction (PCR)-restriction enzyme analysis and differential monoclonal antibody reactivities. To more precisely map the Ly5 gene on the mouse chromosome 1, analytical DNA and protein tests were performed on recombinant inbred strains of mice prepared from SJL/J (Ly5a) and BALB/cke (Ly5b) progenitor strains. Each recombinant inbred strain was characterized to determine whether it carried the Ly5a or Ly5b allele. Both assays, DNA-PCR and protein-immunofluorescence, yielded identical results for each strain examined. Placement of the Ly5 gene with respect to other characterized markers of mouse chromosome 1 for these recombinant inbred mouse strains shows a gene order of Idh-1:Ity:Pep3:[Ly5, Cfh].
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La Jolla Biological Laboratories, California.
Barritt
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Epstein
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Raschke
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D000483:Alleles; D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D005455:Fluorescent Antibody Technique; D005819:Genetic Markers; D006649:Histocompatibility Antigens / Q000235:genetics*; D017493:Leukocyte Common Antigens; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
D015703:Antigens, CD; D005819:Genetic Markers; D006649:Histocompatibility Antigens; D015345:Oligonucleotide Probes; D017493:Leukocyte Common Antigens