On the structure and chromosome location of the 72- and 92-kDa human type IV collagenase genes.
The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. Two members of this family, collagenase and stromelysin, have previously been localized to the long arm of chromosome 11. Here we assign both of the two type IV collagenase genes to human chromosome 16. By sequencing, the 72-kDa gene is shown to consist of 13 exons, 3 more than have been reported for the other members of this gene family. The extra exons encode the amino acids of the fibronectin-like domain which has so far been found in only the 72- and 92-kDa type IV collagenase. The evolutionary relationship among the members of this gene family is discussed.
Collier
I E
IE
Department of Dermatology, Washington University School of Medicine, St. Louis, Missouri 63110.
Bruns
G A
GA
Goldberg
G I
GI
Gerhard
D S
DS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D015342:DNA Probes; D005091:Exons; D005109:Extracellular Matrix; D005260:Female; D006466:Hemopexin / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D008666:Metalloendopeptidases / Q000235:genetics; D003012:Microbial Collagenase / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family
D015342:DNA Probes; D007527:Isoenzymes; D006466:Hemopexin; D008666:Metalloendopeptidases; D003012:Microbial Collagenase