Immobilization of protein on ferromagnetic Dacron.
Ferromagnetic Dacron (polyethyleneterephthalate) is proposed as a matrix to immobilize proteins covalently. Dacron in powder was magnetized by reacting ferrous (Fe+2) and ferric (Fe+3) ions with its hydrazide form at pH 8.3. Ferromagnetic hydrazide Dacron was converted to ferromagnetic azide Dacron and amyloglucosidase (E.C. 3.2.1.3) was covalently bound through the latter group. The catalytic property of the enzyme was preserved (8% of the specific activity estimated for the soluble enzyme) and all the magnetic amyloglucosidase Dacron derivative was recovered by using a magnetic field. No activity was detected in the supernatant.
Leão
A M
AM
Departamento de Bioquímica, Keizo Asami da Universidade Federal de Pernambuco, Cidade Universitária, Recife, Brazil.
Oliveira
E A
EA
Carvalho Júnior
L B
LB
D004800:Enzymes, Immobilized / Q000737:chemistry*; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry*; D005947:Glucose; D006863:Hydrogen-Ion Concentration; D008280:Magnetics; D011093:Polyethylene Terephthalates
D004800:Enzymes, Immobilized; D011093:Polyethylene Terephthalates; D005087:Glucan 1,4-alpha-Glucosidase; D005947:Glucose