title: Evaluation of use of progesterone to counteract zearalenone toxicosis during early pregnancy in gilts.
abstract: It has been shown that zearalenone disrupts early pregnancy in swine without altering intrauterine content of estradiol 17 beta or progesterone, embryo migration, or estradiol-17 beta synthesis by blastocysts. However, serum concentrations of progesterone were reduced 2 to 3 weeks after mating in gilts that ingested zearalenone. Therefore, progesterone was administered to gilts during early pregnancy to determine whether it could counteract the detrimental actions of zearalenone on embryonic development. Thirty-two crossbred gilts (Hampshire x Chester White x Yorkshire x Duroc) were assigned randomly to a 2 x 2 factorial arrangement of treatments: zearalenone (Z); zearalenone plus progesterone (ZP); progesterone (P); or control (C). From postmating days 4 to 15, Z- and ZP-treated gilts were fed 1 mg of Z/kg of body weight, and P-treated and C gilts were fed ethanol as vehicle in a corn-soybean diet. On postmating days 3 to 15, P- and ZP-treated gilts were injected IM with 100 mg of progesterone, and C and Z-treated gilts were injected with progesterone carrier (15% ethanol, 15% benzyl alcohol, 70% propylene glycol). Blood was collected from gilts by puncture of the jugular vein daily from days 3 to 15, on alternate days from days 17 to 31, and then twice weekly until the end of the experiment. Fetal development was assessed in Z- and ZP-treated gilts on postmating day 47.6 +/- 2.9 by cesarean section and in P-treated and C gilts at slaughter on postmating days 51.2 +/- 3.2. Serum concentrations of progesterone in P-treated gilts were greater on days 7 to 8, 10 to 15, 17, and 19 than in C gilts. Serum concentrations of progesterone were greater on days 8, 10, and 12 in ZP-treated than in C gilts. However, serum concentrations of progesterone were lower in ZP-treated gilts than in C gilts on postmating days 19 to 31.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Animal Sciences, Purdue University, West Lafayette, IN 47907.
authors.forename: M L
authors.initials: ML
authors.lastname: Green
authors.forename: D K
authors.initials: DK
authors.lastname: Stouffer
authors.forename: A B
authors.initials: AB
authors.lastname: Scheidt
authors.forename: G G
authors.initials: GG
authors.lastname: Long
authors.forename: M A
authors.initials: MA
authors.lastname: Diekman
mesh_terms: D000818:Animals; D003338:Corpus Luteum / Q000187:drug effects / Q000502:physiology; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005069:Evaluation Studies as Topic; D005260:Female; D011247:Pregnancy; D011248:Pregnancy Complications / Q000139:chemically induced / Q000517:prevention & control / Q000662:veterinary*; D011256:Pregnancy Outcome / Q000662:veterinary; D011270:Pregnancy, Animal / Q000187:drug effects*; D011374:Progesterone / Q000097:blood / Q000494:pharmacology / Q000627:therapeutic use*; D011897:Random Allocation; D013552:Swine; D013553:Swine Diseases / Q000139:chemically induced* / Q000517:prevention & control; D015025:Zearalenone / Q000037:antagonists & inhibitors / Q000506:poisoning*
chemical_list: D011374:Progesterone; D015025:Zearalenone