title: Biochemical and immunohistochemical evidence for selective expression of novel epithelial lipoxygenases.
abstract: Human tracheal epithelial cells contain an arachidonate 15-lipoxygenase, while the same cells from animals (including bovine, ovine, canine, porcine) cells express a 12-lipoxygenase. The epithelial 12-lipoxygenase is antigenically related to the leukocyte 12-lipoxygenase but is biochemically distinct from platelet and leukocyte forms of the enzyme, in that it is more efficient at metabolizing a wider array of fatty acid substrates. We have suggested that this lipoxygenase heterogeneity may provide a basis for different functional roles for the enzyme in different cell types. In addition, animal epithelial 12-lipoxygenase and human epithelial 15-lipoxygenase are antigenically related and have similar but distinct distributions in the lung. Our findings might suggest that the species diversity for epithelial lipoxygenases represents molecular divergence within a family of closely related genes with perhaps closely related functions.
authors.affiliation: Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
authors.forename: V R
authors.initials: VR
authors.lastname: Shannon
authors.forename: J R
authors.initials: JR
authors.lastname: Hansbrough
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: N
authors.initials: N
authors.lastname: Ueda
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
authors.forename: M J
authors.initials: MJ
authors.lastname: Holtzman
mesh_terms: D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000032:analysis* / Q000276:immunology; D001093:Arachidonate 15-Lipoxygenase / Q000032:analysis* / Q000276:immunology; D002417:Cattle; D004285:Dogs; D004790:Enzyme Induction; D004848:Epithelium / Q000201:enzymology*; D006801:Humans; D012756:Sheep; D013045:Species Specificity; D013552:Swine; D014132:Trachea / Q000201:enzymology
chemical_list: D001092:Arachidonate 12-Lipoxygenase; D001093:Arachidonate 15-Lipoxygenase