[DNA-breaking action of ascorbic acid in the presence of Cu(II)/demethylcantharic acid complex].
Effects of various factors on the DNA-breaking action of ascorbic acid in the presence of Cu(II)/demethylcantharic acid complex were studied in phosphate buffer (pH 7.40), at 37.0 +/- 0.1 degrees C. It was shown that .OH produced from Fenton reaction of H2O2 which can be formed from the oxidation of ascorbic acid was responsible for the DNA-breaking action of ascorbic acid and the simple Fenton reaction cannot explain all the results.
Li
P H
PH
Physical Chemistry Department, School of Pharmaceutical Sciences, Beijing Medical University.
Pang
Y H
YH
D001205:Ascorbic Acid / Q000494:pharmacology*; D002614:Chelating Agents / Q000494:pharmacology*; D003300:Copper / Q000494:pharmacology*; D004249:DNA Damage; D011479:Protamines / Q000235:genetics
D002614:Chelating Agents; D011479:Protamines; D003300:Copper; D001205:Ascorbic Acid