title: 2.5S nerve growth factor enhances survival, phagocytosis, and superoxide production of murine neutrophils.
abstract: The effect of 2.5S nerve growth factor (NGF) on survival, phagocytosis, and superoxide production of murine neutrophils was examined and compared with the effects of interleukin-3 (IL-3) and recombinant GM colony-stimulating factor (rGM-CSF). NGF enhanced the viability of neutrophils isolated from peripheral blood and peritoneal cavity in a dose-dependent way. IL-3 50 U/mL had no effect. rGM-CSF 50 U/mL had an effect similar to that of 50 ng/mL NGF. NGF also enhanced the phagocytosis of hydrophilic microspheres by peritoneal neutrophils, and the activity of NGF was greater than that of IL-3 or rGM-CSF. NGF enhanced the superoxide production induced by phorbol 12-myristate 13-acetate (PMA), which acts at postreceptor sites, and that induced by opsonized zymosan, a receptor-mediated ligand. The stimulating activity of NGF was largely comparable to that of rGM-CSF. The present data show that NGF bound to neutrophils enhances their survival, phagocytosis, and superoxide production. Thus, we postulate that NGF plays an important role in the inflammatory processes.
authors.affiliation: Department of Veterinary Surgery, College of Agriculture, University of Osaka Prefecture, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kannan
authors.forename: H
authors.initials: H
authors.lastname: Ushio
authors.forename: H
authors.initials: H
authors.lastname: Koyama
authors.forename: M
authors.initials: M
authors.lastname: Okada
authors.forename: M
authors.initials: M
authors.lastname: Oikawa
authors.forename: T
authors.initials: T
authors.lastname: Yoshihara
authors.forename: M
authors.initials: M
authors.lastname: Kaneko
authors.forename: H
authors.initials: H
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000166:cytology / Q000187:drug effects; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D051379:Mice; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis / Q000187:drug effects* / Q000502:physiology; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D007377:Interleukin-3; D009414:Nerve Growth Factors; D013481:Superoxides; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D013755:Tetradecanoylphorbol Acetate