Stabilization of human rhinovirus serotype 2 against pH-induced conformational change by antiviral compounds.
Four WIN compounds with anti-picornavirus activities were tested for their ability to stabilize human rhinovirus serotype 2 (HRV-2) against low pH-induced conformational changes in vitro, as determined by specific immunoprecipitation. These results were compared to the minimal inhibitory concentration (MIC) as measured in a plaque reduction assay. A direct relationship was observed between the concentration of the compound that prevented the low pH-induced conformational changes and the MIC, indicating that stabilization is an important element in the mode of action of these drugs against HRV-2.
Gruenberger
M
M
Institute of Biochemistry, University of Vienna, Austria.
Pevear
D
D
Diana
G D
GD
Kuechler
E
E
Blaas
D
D
D000998:Antiviral Agents / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007555:Isoxazoles / Q000494:pharmacology*; D011233:Precipitin Tests; D011487:Protein Conformation; D012229:Rhinovirus / Q000145:classification / Q000187:drug effects*; D012703:Serotyping; D014764:Viral Proteins / Q000737:chemistry*
D000998:Antiviral Agents; D007555:Isoxazoles; D014764:Viral Proteins; C045978:disoxaril