Surveys of human enterotoxigenic Escherichia coli from three different geographical areas for possible colonization factors.
Enterotoxigenic Escherichia coli (ETEC) from Burma, central Africa (Rwanda and Zaire) and Peru, were screened by enzyme-linked immunoassays for the colonization factor antigens (CFAs) and putative colonization factors (PCFs): CFA/I, CFA/II, which consists of three coli surface-associated (CS) antigens, CS1, CS2 and CS3, CFA/III, CFA/IV (CS4, CS5, CS6), CS7, PCFO9, PCFO159. H4, PCFO166, and CS17. The highest proportion of ETEC with identifiable colonization factors (71%) were found in the strains from Burma, which were mainly positive for CFA/I (38%), but strains producing CFA/II (4%), CFA/IV (11%), CS7 (10%), CS17 (4%), PCFO159, H4 (2%) and PCFO166 (2%) were also found. Sixty-nine percent of the ETEC from central Africa were positive for known colonization factors. While CFA/I positive strains were important (12%), a higher number of ETEC producing CFA/IV (33%) and CS17 (24%) were found. Fifty-two percent of the Peruvian strains produced identifiable colonization factors. The largest group of strains produced antigens of the CFA/IV complex (17%), while ETEC producing CFA/II (6%), CFA/III and CS6 (2%), CS7 (6%), PCFO9 (6%), PCFO166 (8%) and CS17 (7%) were also found. These surveys show that there is a considerable variation in the proportions and types of colonization factor found in different geographical areas. From 29 to 48% of the ETEC did not possess an identifiable colonization factor. These were particularly of the LT only producing type. These results have important implications for vaccine formulation.
McConnell
M M
MM
Division of Enteric Pathogens, Central Public Health Laboratory, Colindale, London.
Hibberd
M L
ML
Penny
M E
ME
Scotland
S M
SM
Cheasty
T
T
Rowe
B
B
D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis*; D015023:Democratic Republic of the Congo; D003968:Diarrhea, Infantile / Q000382:microbiology; D004768:Enterotoxins / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000145:classification / Q000276:immunology* / Q000378:metabolism; D004927:Escherichia coli Infections / Q000382:microbiology; D036781:Fimbriae Proteins; D006801:Humans; D007223:Infant; D002052:Myanmar; D010568:Peru; D012432:Rwanda; D012703:Serotyping
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004768:Enterotoxins; C022924:colonization factor antigens; D036781:Fimbriae Proteins