title: [Isolation of Cryptosporidium sp. oocysts from human fecal samples].
abstract: Experiments using two sequential discontinuous sucrose gradients, performed with 12%, 18%, 21% and 24% solutions, for the isolation of Cryptosporidium sp. oocysts from human fecal samples were undertaken. The sucrose gradients were centrifuged at 4000 rpm during 20 min. at 10 degrees C or room temperature. After that, 3 bands were observed. Oocysts were recovered mainly from the second band (75.5% after the first gradient, and 44.4% in the next). The two sequential discontinuous sucrose gradients would permit an efficient isolation of Cryptosporidium sp. oocysts from human fecal samples.
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Chile, Santiago.
authors.forename: R
authors.initials: R
authors.lastname: Mercado
authors.forename: E
authors.initials: E
authors.lastname: Araya
authors.forename: E
authors.initials: E
authors.lastname: Espinoza
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D003458:Cryptosporidium / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D006801:Humans; D007223:Infant
vernacular_title: Aislamiento de ooquistes de Cryptosporidium sp. de muestras fecales humanas.