Neuronal protein gene product 9.5 (IEF SSP 6104) is expressed in cultured human MRC-5 fibroblasts of normal origin and is strongly down-regulated in their SV40 transformed counterparts.
Neuronal protein gene product 9.5 (PGP 9.5) most likely identical to ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1) has been reported to be expressed almost exclusively in neuronal and neuroendocrine tissues. By two-dimensional (2D) immunoblotting, comigration and microsequencing of proteins recovered from 2D gels we have identified PGP 9.5 UCH-L1 as polypeptide IEF SSP 6104 (Mr = 27,000, pI = 5.49) in the comprehensive 2D gel cellular protein database of human embryonal lung MRC-5 fibroblasts [(1989) Electrophoresis 10, 76 115; (1990) Electrophoresis 11, 1072 1113]. This protein is expressed at high levels in quiescent and proliferating cultured normal fibroblasts and is strongly down-regulated (about 10 times) in their transformed counterparts.
Honoré
B
B
Institute of Medical Biochemistry, Aarhus University, Denmark.
Rasmussen
H H
HH
Vandekerckhove
J
J
Celis
J E
JE
D000595:Amino Acid Sequence; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D005347:Fibroblasts / Q000378:metabolism* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000096:biosynthesis / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics; D013869:Thiolester Hydrolases / Q000235:genetics; D043222:Ubiquitin Thiolesterase
D007527:Isoenzymes; D009479:Neuropeptides; D013869:Thiolester Hydrolases; D043222:Ubiquitin Thiolesterase