title: Bone morphogenesis of rabbit bone morphogenetic protein-bound hydroxyapatite-fibrin composite.
abstract: Hydroxyapatite (HAP) and fibrin have been implanted in patients and observed to be well tolerated in orthotopic sites. This is a report on a composite of HAP, fibrin, and rabbit bone morphogenetic protein and insoluble noncollagenous protein (BMP-iNCP). Drill holes in the femoral condyles of rabbits were packed with granulated HAP (200 mg), fibrin (0.3 ml), BMP-iNCP (5 mg), or various combinations of the two. The fibrin consisted mainly of sterilized human fibrinogen and thrombin, and BMP-iNCP was prepared from demineralized rabbit cortical bone. New bone formation was observed at one, two, four, and eight weeks after implantation. The BMP-iNCP augmented new bone formation in rabbit femoral condyles. Fibrin made the composite easier to manipulate and did not inhibit osteogenesis at any period. The composites of HAP with BMP-iNCP and of HAP with BMP-iNCP and fibrin produced higher yields of new bone than fibrin alone or HAP alone.
authors.affiliation: Department of Orthopaedic Surgery, Nagoya University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Kawamura
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Iwata
authors.forename: T
authors.initials: T
authors.lastname: Miura
mesh_terms: D000818:Animals; D019485:Bone Morphogenetic Proteins; D001842:Bone and Bones / Q000187:drug effects; D003188:Composite Resins / Q000494:pharmacology / Q000627:therapeutic use*; D017886:Durapatite; D005337:Fibrin / Q000494:pharmacology / Q000627:therapeutic use*; D006133:Growth Substances / Q000494:pharmacology / Q000627:therapeutic use*; D006882:Hydroxyapatites / Q000627:therapeutic use; D010012:Osteogenesis / Q000187:drug effects*; D011506:Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D011817:Rabbits
chemical_list: D019485:Bone Morphogenetic Proteins; D003188:Composite Resins; D006133:Growth Substances; D006882:Hydroxyapatites; D011506:Proteins; D005337:Fibrin; D017886:Durapatite