Cyclic adenosine 3',5'-monophosphate-dependent phosphorylation of HMG 14 inhibits its interactions with nucleosomes.
The high mobility group protein HMG 14, which is preferentially associated with nucleosomes containing active gene sequences, is phosphorylated on different sites according to the tissue and stimulus being studied. In the thyroid, HMG 14 displays TSH-dependent phosphorylation that is mediated by cAMP-dependent protein kinase (A-kinase). We have, therefore, studied how phosphorylation of HMG 14 on its major and minor A-kinase sites (Ser-6 and -24) affects its interactions with nucleosomes and various forms of DNA, since this could reflect a means of regulating its function of binding to active chromatin. Approximately twice as much Ser-6 phospho- and 4 times as much Ser-6,24 diphospho-HMG 14 were required to produce the same degree of nucleosome band displacement as that caused by native unphosphorylated HMG 14. Phosphorylation also reduced the ability of HMG 14 to protect the ends of nucleosomal DNA from thermal denaturation. When the electrophoretic mobility of naked DNA was examined, the Ser-6 phospho-HMG 14 was about half as effective as native HMG 14 in retarding the various forms of double stranded DNA, and Ser-6,24 diphospho-HMG 14 was even less effective. Our data demonstrate that electrostatic interactions between DNA and basic amino acids in two highly conserved regions (residues 1-5 and 16-27) can be modulated by phosphorylation at Ser-6 and Ser-24. The ability of mammalian HMG 14, but not HMG 17, to display hormone-dependent phosphorylation may indicate a route for differentially modulating their binding to transcriptionally active chromatin.
Spaulding
S W
SW
Department of Medicine, Buffalo Veterans Administration Medical Center, New York 14215.
Fucile
N W
NW
Bofinger
D P
DP
Sheflin
L G
LG
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002645:Chickens; D000242:Cyclic AMP / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D004563:Electrochemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006609:High Mobility Group Proteins / Q000378:metabolism*; D009707:Nucleosomes / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D010768:Phosphoserine / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D013961:Thyroid Gland / Q000378:metabolism / Q000648:ultrastructure; D013972:Thyrotropin / Q000494:pharmacology
D006609:High Mobility Group Proteins; D009707:Nucleosomes; D010750:Phosphoproteins; D010768:Phosphoserine; D013972:Thyrotropin; D004247:DNA; D000242:Cyclic AMP; D011494:Protein Kinases