Nit-3, the structural gene of nitrate reductase in Neurospora crassa: nucleotide sequence and regulation of mRNA synthesis and turnover.
The nit-3 gene of the filamentous fungus Neurospora crassa encodes the enzyme nitrate reductase, which catalyzes the first reductive step in the highly regulated nitrate assimilatory pathway. The nucleotide sequence of nit-3 was determined and translates to a protein of 982 amino acid residues with a molecular weight of approximately 108 kDa. Comparison of the deduced nit-3 protein sequence with the nitrate reductase protein sequences of other fungi and higher plants revealed that a significant amount of homology exists, particularly within the three cofactor-binding domains for molybdenum, heme and FAD. The synthesis and turnover of the nit-3 mRNA were also examined and found to occur rapidly and efficiently under changing metabolic conditions.
Okamoto
P M
PM
Department of Biochemistry, Ohio State University, Columbus 43210.
Fu
Y H
YH
Marzluf
G A
GA
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006418:Heme / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D008982:Molybdenum / Q000378:metabolism; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics*; D009566:Nitrates / Q000378:metabolism; D012331:RNA, Fungal / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D009566:Nitrates; D012331:RNA, Fungal; D012333:RNA, Messenger; D005182:Flavin-Adenine Dinucleotide; D006418:Heme; D008982:Molybdenum; D009565:Nitrate Reductases; D050901:Nitrate Reductase