title: Nucleotide sequence of the gelatinase gene (gelE) from Enterococcus faecalis subsp. liquefaciens.
abstract: The gene coding for gelatinase (also called metalloendopeptidase II; microbial proteinase, EC 3.4.24.4) of Enterococcus faecalis subsp. liquefaciens strain OG1-10 was cloned in an Escherichia coli-Enterococcus shuttle vector, and its nucleotide sequence was determined. The DNA sequence encodes one large open reading frame (ORF) with 509 amino acid residues. The ORF contains a signal sequence in its N-terminal region, whereas the N-terminal amino acid sequence determined from the purified extracellular proteinase starts at residue 192 deduced from the ORF. This implies that the gelatinase is synthesized as a prepropolypeptide or prezymogen. The mature gelatinase contains 318 amino acid residues (molecular weight, 34,582) and has significant homology with neutral proteinases from Bacillus species and elastase from Pseudomonas aeruginosa.
authors.affiliation: Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor 48109.
authors.forename: Y A
authors.initials: YA
authors.lastname: Su
authors.forename: M C
authors.initials: MC
authors.lastname: Sulavik
authors.forename: P
authors.initials: P
authors.lastname: He
authors.forename: K K
authors.initials: KK
authors.lastname: Makinen
authors.forename: P L
authors.initials: PL
authors.lastname: Makinen
authors.forename: S
authors.initials: S
authors.lastname: Fiedler
authors.forename: R
authors.initials: R
authors.lastname: Wirth
authors.forename: D B
authors.initials: DB
authors.lastname: Clewell
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology; D001483:Base Sequence; D003001:Cloning, Molecular; D013293:Enterococcus faecalis / Q000201:enzymology / Q000235:genetics*; D018093:Gelatinases; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010196:Pancreatic Elastase / Q000032:analysis; D010434:Pepsin A / Q000032:analysis / Q000235:genetics*; D011549:Pseudomonas / Q000201:enzymology
chemical_list: D010196:Pancreatic Elastase; D010434:Pepsin A; D018093:Gelatinases