Class II-antigen-negative patient and mutant B-cell lines represent at least three, and probably four, distinct genetic defects defined by complementation analysis.
Expression of class II major histocompatibility complex antigens in defective B-lymphoblastoid cell lines from patients with class II antigen deficiency and from in vitro mutants generated with the same phenotype was studied. By heterogenetic fusion experiments, at least three, and probably four, complementation groups were defined. Furthermore, clone 13 (a DR-, DP-, but DQ+ cell line) appeared to belong to the RJ2.2.5 complementation group, for which all other members are DR-, DP-, and also DQ-. Thus, it is hypothesized that the cell lines of this group lack the activity of a gene that can differentially regulate the DR/DP and the DQ promoters.
Bénichou
B
B
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
Strominger
J L
JL
D001402:B-Lymphocytes / Q000276:immunology*; D002461:Cell Line, Transformed; D015870:Gene Expression; D005786:Gene Expression Regulation; D005802:Genes, MHC Class II; D005816:Genetic Complementation Test; D006682:HLA-DP Antigens / Q000235:genetics; D006683:HLA-DQ Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics; D004854:Herpesvirus 4, Human; D000949:Histocompatibility Antigens Class II / Q000172:deficiency* / Q000235:genetics; D006801:Humans; D016296:Mutagenesis; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D011401:Promoter Regions, Genetic
D006682:HLA-DP Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II