title: Morphological changes in the endocrine pancreas of mice after treatment with streptozotocin combined with cyclophosphamide and neurotropin.
abstract: The effect of neurotropin (NSP) in combination with streptozotocin (STZ) and cyclophosphamide (CY) on blood glucose and pancreatic histopathology on day 7 and day 14 after the initiation of the treatment was studied in C57Bl/6 male mice. STZ (40 mg/kg) and NSP (1 mg/kg) were applied intraperitoneally on five consecutive days and CY (150 mg/kg)--twice on day 1 and day 3. In single B cells dilatation of the endoplasmic reticulum was found. On day 7 in proximity to some endocrine cells in the mice treated with STZ, STZ + CY + NSP and STZ + CY macrophages were observed. On day 14 lymphocytic infiltration of the islets was demonstrated only in the groups of mice injected with STZ, STZ + CY while in the group treated with the combination STZ + CY + NSP no infiltration was seen. All experimental groups showed no biochemical evidence for hyperglycemia probably due to the mild destruction of a small number of B cells. The results indicate that NSP might possess a restorative action on insulitis induced by multiple low dose streptozotocin administration in mice.
authors.affiliation: Academy of Medicine, Department of Anatomy, Histology and Embryology, Sofia, Bulgaria.
authors.forename: R
authors.initials: R
authors.lastname: Jablenska
authors.forename: P
authors.initials: P
authors.lastname: Popova
authors.forename: G
authors.initials: G
authors.lastname: Guencheva
authors.forename: J
authors.initials: J
authors.lastname: Duhault
authors.forename: M
authors.initials: M
authors.lastname: Lesourd
authors.forename: P
authors.initials: P
authors.lastname: Petkov
mesh_terms: D000818:Animals; D001786:Blood Glucose; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000494:pharmacology*; D004338:Drug Combinations; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010179:Pancreas / Q000187:drug effects* / Q000648:ultrastructure; D011134:Polysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D013311:Streptozocin / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D001786:Blood Glucose; D004338:Drug Combinations; D011134:Polysaccharides; C010491:neurotropin; D013311:Streptozocin; D003520:Cyclophosphamide