Inhibition of gastric mucosal laminin receptor by Helicobacter pylori lipopolysaccharide.
Laminin receptor was isolated from gastric epithelial cell membrane by the procedure involving membrane solubilization with octylglucoside followed by affinity chromatography on laminin-coupled Sepharose. The receptor protein, eluted from the matrix with cation-free EDTA buffer, yielded on SDS-PAGE a single 67kDa band. After radioiodination, the protein was incorporated into liposomes which displayed specific affinity toward the laminin-coated surface. The binding of liposomal receptor to the laminin-coated surface was inhibited by lipopolysaccharide from H.pylori. The inhibitory effect was proportional to the concentration of lipopolysaccharide up to 50 micrograms/ml at which point a 96% decrease in the receptor binding occurred. It is suggested that a similar process may account for the loss of mucosal integrity in the pathogenesis of H. pylori associated gastric disease.
Slomiany
B L
BL
Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
Piotrowski
J
J
Sengupta
S
S
Slomiany
A
A
D000818:Animals; D001419:Bacteria / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D002846:Chromatography, Affinity; D004848:Epithelium / Q000276:immunology; D005753:Gastric Mucosa / Q000276:immunology*; D005960:Glucosides; D016480:Helicobacter pylori / Q000276:immunology*; D007700:Kinetics; D007797:Laminin / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008081:Liposomes; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011971:Receptors, Immunologic / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D017474:Receptors, Laminin
D005960:Glucosides; D007797:Laminin; D008070:Lipopolysaccharides; D008081:Liposomes; D011971:Receptors, Immunologic; D017474:Receptors, Laminin; C018619:octyl-beta-D-glucoside