Comparison of protective immunity elicited by recombinant vaccinia viruses that synthesize E or NS1 of Japanese encephalitis virus.
Immunization with recombinant vaccinia viruses that specified the synthesis of Japanese encephalitis virus (JEV) glycoproteins protected mice from a lethal intraperitoneal challenge with JEV. Recombinants which coexpressed the genes for the structural glycoproteins, prM and E, elicited high levels of neutralizing (NEUT) and hemagglutination inhibiting (HAI) antibodies in mice and protected mice from a lethal challenge by JEV. Recombinants expressing only the gene for the nonstructural glycoprotein, NS1, induced antibodies to NS1 but provided low levels of protection from a similar challenge dose of JEV. Antibodies to the NS3 protein in postchallenge sera, representing the degree of infection with challenge virus, were inversely correlated to NEUT and HAI titers and levels of protection. These results indicate that although vaccinia recombinants expressing NS1 can provide some protection from lethal JEV infection, recombinants expressing prM and E elicited higher levels of protective immunity.
Konishi
E
E
Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06510.
Pincus
S
S
Fonseca
B A
BA
Shope
R E
RE
Paoletti
E
E
Mason
P W
PW
D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D005814:Genes, Viral; D006367:HeLa Cells; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007114:Immunization; D051379:Mice; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009838:Oligodeoxyribonucleotides; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology*; D014709:Vero Cells; D014758:Viral Core Proteins / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins / Q000235:genetics*; D014779:Virus Replication
D009838:Oligodeoxyribonucleotides; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins