The multispecific cell adhesion molecule CD44 is represented in reticulocyte cDNA.
Identified originally as erythrocyte p80, whose expression is down regulated by the Lutheran inhibitor gene (In[Lu]), the In(Lu) related-p80 glycoprotein represents the red cell isoform of the human cell adhesion/recognition molecule CD44. The presence of a CD44 transcript within a reticulocyte cDNA library was indicated by the PCR amplification of an appropriately sized product generated by a pair of deoxyoligonucleotide primers derived from CD44 cDNA sequence. The amplified product was subsequently utilized to screen and isolate a positively hybridizing full-length reticulocyte cDNA clone (RETIC CD44) that contained an 1809 base pair insert that was DNA sequenced by the dideoxy chain termination method. This first isolate of a reticulocyte CD44 cDNA appears to be generated by a combination of RNA processing events that includes production of 3' mRNA heterogeneity by utilization of multiple poly(A) sites. Remarkably, the 3' untranslated (3'UT) region of this mRNA, encoding a prototypic hematopoietic CD44 isoform, has been previously reported present on only a transcript that encodes a CD44 epithelial isoform.
Harn
H J
HJ
Department of Pathology, Duke University Medical Center, Durham, NC 27710.
Isola
N
N
Cooper
D L
DL
D000595:Amino Acid Sequence; D001483:Base Sequence; D002880:Chromosomes, Human, Pair 11; D004247:DNA / Q000097:blood / Q000235:genetics; D005786:Gene Expression Regulation; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D016201:Receptors, Lymphocyte Homing / Q000235:genetics*; D015183:Restriction Mapping; D012156:Reticulocytes / Q000276:immunology*; D012689:Sequence Homology, Nucleic Acid
D015345:Oligonucleotide Probes; D016201:Receptors, Lymphocyte Homing; D004247:DNA