title: Peripheral nerve regeneration is impeded by interleukin-1 receptor antagonist released from a polymeric guidance channel.
abstract: Interleukin-1 receptor antagonist (IL-1ra), a true antagonist of the interleukin-1 (IL-1) receptors, is released by activated macrophages and binds specifically to the IL-1 receptors without triggering IL-1 effects. Following peripheral nerve axotomy, activated macrophages release IL-1, which induces the expression of nerve growth factor (NGF) mRNA in Schwann cells. IL-1ra may therefore impede peripheral nerve regeneration by blocking the NGF-mediated effect of IL-1. Peripheral nerve regeneration occurring through polymeric guidance channels releasing IL-1ra was investigated in a 4-mm gap transected mouse sciatic nerve model. Cohorts of five animals were implanted with tubes releasing either bovine serum albumin (BSA), BSA with IL-1ra, or BSA with deactivated IL-1ra (dIL-1ra) for 4 weeks. In vitro release kinetics indicated that after an initial burst, IL-1ra release was linear for the next 3 1/2 weeks. Following implantation of a polymeric guidance channel, a regenerated cable bridged the nerve gap in all animals. The cables were similar in size and were composed of nerve microfascicles containing both unmyelinated and myelinated axons in association with their Schwann cells. Tissue regenerated in tubes releasing BSA-IL-1ra contained, however, significantly fewer myelinated and unmyelinated axons and blood vessels than did tubes releasing BSA alone or BSA-dIL-1ra. We conclude that a naturally occurring antagonist of IL-1 receptors impedes peripheral nerve regeneration, suggesting that macrophages play an essential role in controlling peripheral nerve regeneration through the release of stimulatory and/or inhibitory molecules.
authors.affiliation: Section of Artificial Organs, Biomaterials and Cellular Technology, Brown University, Providence, Rhode Island 02912.
authors.forename: V
authors.initials: V
authors.lastname: Guénard
authors.forename: C A
authors.initials: CA
authors.lastname: Dinarello
authors.forename: P J
authors.initials: PJ
authors.lastname: Weston
authors.forename: P
authors.initials: P
authors.lastname: Aebischer
mesh_terms: D000818:Animals; D004343:Drug Implants; D005260:Female; D053590:Interleukin 1 Receptor Antagonist Protein; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D009416:Nerve Regeneration / Q000187:drug effects*; D011145:Polyvinyls; D011506:Proteins / Q000494:pharmacology*; D011994:Recombinant Proteins; D012584:Sciatic Nerve / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D012795:Sialoglycoproteins
chemical_list: D004343:Drug Implants; C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D011145:Polyvinyls; D011506:Proteins; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; C016438:ethylenevinylacetate copolymer