title: Murine epidermal antigen-presenting cells in primary and secondary T-cell proliferative responses to herpes simplex virus in vitro.
abstract: The role of epidermal Langerhans' cells in infection with herpes simplex virus (HSV) was investigated using a culture system that supports antigen-specific primary and secondary T-cell proliferative responses. Epidermal cell suspensions were capable of restimulating the response of in vivo primed T cells to UV-inactivated HSV. This capability was also present in cell suspensions enriched for Langerhans' cells, but was abrogated by the depletion of I-A-bearing cells. The magnitude, kinetics and phenotype of the responding cells were similar to those elicited when HSV was presented to primed T cells by antigen-presenting cells from the spleen. In marked contrast, whereas splenic antigen-presenting cells induced strong antigen-specific proliferation of unprimed T cells (primarily of the helper phenotype), Langerhans' cells failed to invoke any detectable reaction of such cells.
authors.affiliation: Department of Microbiology, The Medical School, University of Bristol, U.K.
authors.forename: N A
authors.initials: NA
authors.lastname: Williams
authors.forename: T J
authors.initials: TJ
authors.lastname: Hill
authors.forename: D C
authors.initials: DC
authors.lastname: Hooper
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D004817:Epidermis / Q000276:immunology*; D005260:Female; D007156:Immunologic Memory; D007801:Langerhans Cells / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008808:Mice, Inbred CBA; D018139:Simplexvirus / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000956:Antigens, Viral