title: Osmotic swelling of endocytic compartments induced by internalized sucrose is restricted to mature lysosomes in cultured mammalian cells.
abstract: To determine which endocytic compartments are sensitive to sucrose-induced osmotic swelling, CHO and Vero cells were cultured for 1-3 days in media containing 0.03 to 0.05 M sucrose. (Sucrose is internalized but not digested by these cells.) To immunolocalize late endocytic compartments, cells were fixed with formaldehyde and labeled with antibodies against the 215-kDa mannose 6-phosphate receptor (prelysosomal compartment) and LAMP-1 and -2 (mature lysosomes). Early endosomes were labeled by a 2-min uptake of lucifer yellow, mature lysosomes by greater than or equal to 16-h uptake of lucifer yellow followed by a 2-h chase. The data showed that sucrose induced swelling of mature lysosomes only (mannose 6-phosphate receptor negative, LAMP-1 and LAMP-2 positive); early endosomes and the prelysosomal compartment were not affected by the presence of sucrose, i.e., osmotically swollen. Accumulation of lucifer yellow in the swollen compartment was insensitive to cycloheximide. These results suggest, by inference, that the complement of membrane transport proteins that regulate the osmotic properties of endocytic organelles must be discontinuously distributed along the endocytic pathway.
authors.affiliation: Department of Biochemistry and Nutrition, Virginia Polytechnic Institute and State University, Blacksburg 24061.
authors.forename: K
authors.initials: K
authors.lastname: DeCourcy
authors.forename: B
authors.initials: B
authors.lastname: Storrie
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000378:metabolism; D007546:Isoquinolines / Q000378:metabolism; D008247:Lysosomes / Q000187:drug effects / Q000502:physiology*; D008858:Microscopy, Phase-Contrast; D009995:Osmosis; D017527:Receptor, IGF Type 2; D011956:Receptors, Cell Surface / Q000032:analysis; D013395:Sucrose / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism; D014709:Vero Cells
chemical_list: D005456:Fluorescent Dyes; D007546:Isoquinolines; D017527:Receptor, IGF Type 2; D011956:Receptors, Cell Surface; D013395:Sucrose; C017475:lucifer yellow; D003513:Cycloheximide