A novel indicator system allows detection of point mutations conferring a Ner- phenotype of phage Mu.
A plasmid-based indicator system was constructed which allows the easy detection of point mutations within the ner gene of bacteriophage Mu and its regulatory region. Five mutants have been sequenced exhibiting point mutations within the ner gene itself or its upstream region. These data are discussed with respect to the activity of the Ner protein.
Mayer
E
E
Lehrstuhl für Genetik, Universität Bayreuth, F.R.G.
Mummenthey
K
K
Schumann
W
W
D000595:Amino Acid Sequence; D010583:Bacteriophage mu / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral; D005809:Genes, Regulator; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D012097:Repressor Proteins / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*; D054334:Viral Regulatory and Accessory Proteins
D004279:DNA, Viral; D012097:Repressor Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins