Serotonin (5-HT1c) receptors in pig choroid plexus.
It is very important to find suitable reaction conditions to attain a high specific binding (specific/total binding) in the receptor binding study. Membrane homogenates of pig choroid plexus are known to have exclusively serotonin (5-hydroxytryptamine, 5-HT) receptor of the subtype 5-HT1c. In this study, we used the membrane preparation of pig choroid plexus tissue and the specific binding of [3H]5-HT was 72-84% to serotonin receptor subtype 5-HT1c, as defined by the inhibition of 1 uM 5-HT, when a radioligand concentration of 0.5 nM of [3H]5-HT was used in the assay. Analysis of the properties of specific [3H]5-HT binding in pig choroid plexus tissue membrane preparation revealed linear Scatchard plots. In Tris-HCl buffer without CaCl2, pargyline or ascorbic acid, high average of affinity dissociation constant (Kd) of 1.3 +/- 0.2 nM (SEM, n = 4) and also a high average of receptor density (Bmax) of 284 +/- 12 fmol/mg of protein were found. Pig choroid plexus proves to be a good material for 5-HT1c receptor binding study.
Yiin
K T
KT
Department of Internal Medicine, Veterans General Hospital-Taichung, Taiwan, R.O.C.
Chen
C F
CF
Kuo
J S
JS
Tsou
C T
CT
Wang
J J
JJ
Song
Y M
YM
Lin
S H
SH
Tseng
L N
LN
Wang
Y S
YS
Lin
W H
WH
D000818:Animals; D002831:Choroid Plexus / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism; D013552:Swine
D011985:Receptors, Serotonin; D012701:Serotonin