Entamoeba histolytica: antigenic characterization of axenic strains from Brazil.
Trophozoites from cultures of Entamoeba histolytica strains isolated and grown axenically in Brazil (ICB-CSP, ICB-462 and ICB-32) were used for immune sera production and for characterization of their antigens by using electrophoretic and glycoproteic profiles, in parallel with a standard strain isolated and kept under axenic conditions in USA (HK-9). Hyperimmune sera, presenting high antibody titers with homologous and heterologous antigens, were obtained. The four strains in study revealed similar and complex electrophoretic and glycoproteic profiles showing polypeptides with molecular weights ranging from 200 to less than 29 kDa. No significant differences were detected between the pathogenic and non-pathogenic strains.
Guimaráes
S
S
Department of Parasitology, Federal Minas Gerais University, Belo Horizonte, Brazil.
Urdaneta
H
H
Silva
E F
EF
Tavares
C A
CA
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D015941:Complement Hemolytic Activity Assay; D004591:Electrophoresis, Polyacrylamide Gel; D004748:Entamoeba histolytica / Q000145:classification / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005856:Germ-Free Life; D007106:Immune Sera; D011952:Receptors, Concanavalin A; D012967:Sodium Dodecyl Sulfate
D000953:Antigens, Protozoan; D007106:Immune Sera; D011952:Receptors, Concanavalin A; D012967:Sodium Dodecyl Sulfate