title: Stabilization of human rhinovirus serotype 2 against pH-induced conformational change by antiviral compounds.
abstract: Four WIN compounds with anti-picornavirus activities were tested for their ability to stabilize human rhinovirus serotype 2 (HRV-2) against low pH-induced conformational changes in vitro, as determined by specific immunoprecipitation. These results were compared to the minimal inhibitory concentration (MIC) as measured in a plaque reduction assay. A direct relationship was observed between the concentration of the compound that prevented the low pH-induced conformational changes and the MIC, indicating that stabilization is an important element in the mode of action of these drugs against HRV-2.
authors.affiliation: Institute of Biochemistry, University of Vienna, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Gruenberger
authors.forename: D
authors.initials: D
authors.lastname: Pevear
authors.forename: G D
authors.initials: GD
authors.lastname: Diana
authors.forename: E
authors.initials: E
authors.lastname: Kuechler
authors.forename: D
authors.initials: D
authors.lastname: Blaas
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007555:Isoxazoles / Q000494:pharmacology*; D011233:Precipitin Tests; D011487:Protein Conformation; D012229:Rhinovirus / Q000145:classification / Q000187:drug effects*; D012703:Serotyping; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D000998:Antiviral Agents; D007555:Isoxazoles; D014764:Viral Proteins; C045978:disoxaril