Augmentation of reverse arthus reaction by mast cells in mice.
Immune complex-induced injury is an important pathogenic factor in antibody-mediated nephritis, systemic lupus erythematosus, rheumatoid arthritis, and other diseases. In this study we investigated the role mast cells in immune complex-mediated injury in mouse skin. Reverse Arthus reaction was induced in mast cell-deficient WBB6F1-W/Wv mice and their congenic controls (WBB6F1(-)+/+). Serial skin sections were evaluated for neutrophil infiltration, edema, and hemorrhage. In WBB6F1-W/Wv mice the neutrophil influx was only 40% and edema 60% of that in congenic controls. Hemorrhage was also significantly reduced in the mast cell-deficient mice. After mast cell reconstitution, the magnitude of the reaction in WBB6F1-W/Wv was equivalent to that in WBB6F1(-)+/+ mice. Mast cell release in reverse Arthus reaction was evaluated by measuring fluorescence intensity after avidin-FITC staining of mast cell granules. There was a 70% decrease in fluorescence intensity. The 5-lipoxygenase inhibitor A-63162 significantly decreased neutrophil accumulation (40%), edema (60%), and hemorrhage in WBB6F1(-)+/+, but not in mast cell-deficient mice. Mast cell reconstitution of WBB6F1-W/Wv mice restored the effect of A-63162. The results indicate that mast cells and their mediators, including leukotrienes, make an important contribution to reverse Arthus reaction.
Zhang
Y
Y
Department of Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110.
Ramos
B F
BF
Jakschik
B A
BA
D000081:Acetamides / Q000494:pharmacology; D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology; D001183:Arthus Reaction / Q000209:etiology*; D015550:Cell Degranulation; D004487:Edema / Q000209:etiology; D006470:Hemorrhage / Q000209:etiology; D007249:Inflammation / Q000209:etiology; D015289:Leukotrienes / Q000502:physiology; D016859:Lipoxygenase Inhibitors; D008407:Mast Cells / Q000502:physiology*; D051379:Mice; D009504:Neutrophils / Q000502:physiology; D010647:Phenyl Ethers; D012867:Skin / Q000276:immunology / Q000473:pathology
D000081:Acetamides; D000936:Antigen-Antibody Complex; D015289:Leukotrienes; D016859:Lipoxygenase Inhibitors; D010647:Phenyl Ethers; C068663:N-hydroxy-N-(1-(4-(phenylmethoxy)phenyl)ethyl)-acetamide