title: A second gene product of the inositol-phospholipid-specific phospholipase C delta subclass.
abstract: Sequence analysis of a inositol-phospholipid-specific phospholipase C (PtdIns-PLC) purified from bovine brain has led to the isolation of a novel cDNA that encodes this protein. While this cDNA contains two introns, these appear to be removed upon transfection of the cDNA into COS-1 cells. The protein transiently expressed in COS-1 cells shows phosphatidylinositol 4,5-bisphosphate hydrolysing activity which distributes preferentially into the particulate fraction. Comparison of the predicted amino acid sequence of this PtdIns-PLC with other known PtdIns-PLCs reveals a high degree of similarity, throughout all of its sequence, with PtdIns-PLC delta. Thus, we believe that the identification of this cDNA represents evidence for multiple functional-gene products within the delta subclass of PtdIns-PLCs.
authors.affiliation: Ludwig Institute for Cancer Research, London, England.
authors.forename: E
authors.initials: E
authors.lastname: Meldrum
authors.forename: R W
authors.initials: RW
authors.lastname: Kriz
authors.forename: N
authors.initials: N
authors.lastname: Totty
authors.forename: P J
authors.initials: PJ
authors.lastname: Parker
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D004247:DNA / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009154:Mutation; D010716:Phosphatidylinositols / Q000378:metabolism*; D014162:Transfection; D010738:Type C Phospholipases / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics
chemical_list: D010716:Phosphatidylinositols; D004247:DNA; D010738:Type C Phospholipases