Effects of sex steroids on beta-endorphin release from rat hypothalamus in vitro.
The effects of sex steroids on immunoreactive beta-endorphin (EP) release from the rat hypothalamus in vitro were examined using a rat hypothalamic perifusion system and an EP RIA. Testosterone (1-100 ng/ml) and estradiol (10-100 pg/ml) stimulated EP release in a dose-dependent manner. Dehydroepiandrosterone (DHEA, 1-100 ng/ml) dose-dependently inhibited EP release. These results indicate an inverse relationship between the acute effect of gonadal sex steroids and that of adrenal androgen on hypothalamic EP release in vitro.
Nakano
Y
Y
Department of Medicine, Tokyo Women's Medical College, Japan.
Suda
T
T
Sumitomo
T
T
Tozawa
F
F
Demura
H
H
D000818:Animals; D003687:Dehydroepiandrosterone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D010477:Perfusion; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000494:pharmacology; D001615:beta-Endorphin / Q000378:metabolism*
D012739:Gonadal Steroid Hormones; D013739:Testosterone; D003687:Dehydroepiandrosterone; D004958:Estradiol; D001615:beta-Endorphin