Autoradiographic localization of [125I]-C-ANP compared to [125I]-ANP in rat tissue.
Whole-body autoradiography demonstrated the different distribution of [125I]-C-ANP and [125I]-ANP to rat tissues. Highest enrichment of radioactivity of both labelled peptides was found in the kidney. In some organs we found remarkable differences between [125I]-ANP and [125I]-C-ANP. In the kidney cortex, especially in the glomeruli, as well as in the endocardium, the zona glomerulosa and the medulla of the adrenal gland, where high levels of radioactivity after [125I]-ANP administration were detected, no or just few radioactivity was found after administration of [125I]-C-ANP. On the other hand in the kidney papilla and the outer subcortical medulla, characteristic blackening was found after [125I]-C-ANP administration. Those differences might be important for the understanding of pharmacological actions of ANP analogues.
Heidemann
F
F
Institute of Pharmacology, Bayer A.G. Research Center, Wuppertal, Federal Republic of Germany.
Steinke
W
W
Pleiss
U
U
Theiss
G
G
Voges
K P
KP
Kazda
S
S
Gerzer
R
R
Stasch
J P
JP
D000311:Adrenal Glands / Q000378:metabolism*; D000313:Adrenal Medulla / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000378:metabolism; D009320:Atrial Natriuretic Factor / Q000032:analysis / Q000493:pharmacokinetics*; D001345:Autoradiography; D004699:Endocardium / Q000378:metabolism; D007457:Iodine Radioisotopes; D007668:Kidney / Q000378:metabolism*; D008297:Male; D010446:Peptide Fragments / Q000493:pharmacokinetics*; D051381:Rats; D014018:Tissue Distribution; D015384:Zona Glomerulosa / Q000378:metabolism
D007457:Iodine Radioisotopes; D010446:Peptide Fragments; C065367:atrial natriuretic factor (4-23)NH2, de-Gln(18)-de-Ser(19)-de-Gly(20,22)-de-Leu(21)-; D009320:Atrial Natriuretic Factor