Characterization of the thoracic duct T-helper cells that co-mediate, with antibody, the rapid expulsion of Trichinella spiralis in adult rats.
Thoracic duct cells that act synergistically with immune serum or antibody to transfer rapid expulsion of a challenge infection with Trichinella spiralis muscle larvae were characterized as OX38+, OX8-, OX22- T helper cells. Protective capacity was confined to the dividing T helpers that appeared on days 3-5 in the thoracic duct of rats during a T. spiralis infection. To realize their intestinal priming potential in recipient rats. MHC-compatibility between donor and recipient rat was required. Fractionation of immune serum with 40% saturated ammonium sulphate left transferable protective activity in both the precipitate and supernatant fractions. Absorption of immune serum with muscle larvae antigen removed the capacity to transfer protection.
Ahmad
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A
James A. Baker Institute for Animal Health, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Bell
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RG
Wang
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CH
Sacuto
F R
FR
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology; D005260:Female; D007106:Immune Sera / Q000276:immunology; D007116:Immunization, Passive; D016130:Immunophenotyping; D008285:Major Histocompatibility Complex / Q000276:immunology; D008297:Male; D051381:Rats; D011922:Rats, Mutant Strains; D016176:T-Lymphocyte Subsets / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D013897:Thoracic Duct / Q000276:immunology*; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000517:prevention & control
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007106:Immune Sera