Immunologic priming with recombinant hepatitis A virus capsid proteins produced in Escherichia coli.
Hepatitis A virus capsid proteins (VP0, VP3, and VP1) have been synthesized in Escherichia coli for use in antigenic and immunogenic analyses. Rabbits immunized with each of these individual recombinant capsid proteins developed a rapid neutralizing antibody response when subsequently challenged with a subimmunogenic dose of whole virus.
Powdrill
T F
TF
Department of Clinical Immunology, Burroughs Wellcome Company, Research Triangle Park, North Carolina 27709.
Johnston
J M
JM
D000818:Animals; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D006508:Hepatitis Antibodies / Q000096:biosynthesis; D006507:Hepatovirus / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D015151:Immunoblotting; D009500:Neutralization Tests; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D006508:Hepatitis Antibodies; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines