title: Indomethacin modulation of monocyte cytokine release following pelvic irradiation for cancer.
abstract: Pelvic irradiation for urogenital cancer reduced monocyte release of tumour necrosis factor alpha (TNF-alpha). Addition of indomethacin to monocyte cultures increased TNF-alpha production after but not before irradiation. E. coli lipopolysaccharide (LPS) increased TNF-alpha release before as well as after radiation therapy and addition of indomethacin to LPS-stimulated monocytes further increased TNF-alpha production following radiotherapy. Spontaneous interleukin-1 (IL-1) release was increased in the cancer patients and was not significantly affected by radiation therapy. LPS increased IL-1 release before as well as after irradiation, but indomethacin did not further change IL-1 secretion. These findings suggest that prostaglandins differentially regulate TNF-alpha and IL-1 release. Administration of cyclo-oxygenase inhibitors during radiation therapy might increase TNF-alpha release in vivo and thereby enhance the host defence against tumours.
authors.affiliation: Central Microbiological Laboratory, Stockholm County Council, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Petrini
authors.forename: G
authors.initials: G
authors.lastname: Wolk
authors.forename: J
authors.initials: J
authors.lastname: Wasserman
authors.forename: I
authors.initials: I
authors.lastname: Vedin
authors.forename: O
authors.initials: O
authors.lastname: Strannegård
authors.forename: H
authors.initials: H
authors.lastname: Blomgren
authors.forename: P L
authors.initials: PL
authors.lastname: Esposti
mesh_terms: D000368:Aged; D004926:Escherichia coli; D005260:Female; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology*; D007375:Interleukin-1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D010388:Pelvis; D011471:Prostatic Neoplasms / Q000532:radiotherapy*; D015203:Reproducibility of Results; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism* / Q000528:radiation effects; D001749:Urinary Bladder Neoplasms / Q000532:radiotherapy
chemical_list: D007375:Interleukin-1; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D007213:Indomethacin