A fission-yeast gene encoding a protein with features of protein-tyrosine-phosphatases.
Degenerate oligonucleotide probes encoding sequences conserved among mammalian protein-tyrosine-phosphatases (PTPases) were used to amplify DNA fragments from a Schizosaccharomyces pombe cDNA library by polymerase chain reaction (PCR) methods. A cloned PCR product predicted peptide sequences similar to those found in PTPases but not identical to any published sequences. A S. pombe gene, designated pyp1+, was identified in a cDNA library with this PCR probe, cloned, and sequenced. The sequence of the gene predicted a 550-amino acid protein with Mr 61,586, which includes amino acid sequences that are highly conserved in mammalian PTPases. Disruption of the pyp1+ gene resulted in viable cells. Overexpression of the pyp1+ gene in S. pombe permitted detection of a protein of apparent Mr 63,000.
Ottilie
S
S
Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138.
Chernoff
J
J
Hannig
G
G
Hoffman
C S
CS
Erikson
R L
RL
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004271:DNA, Fungal / Q000235:genetics; D015870:Gene Expression; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D017027:Protein Tyrosine Phosphatases; D012333:RNA, Messenger / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics*
D004271:DNA, Fungal; D009841:Oligonucleotides; D012333:RNA, Messenger; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases