title: Molecular cloning of a novel isolate of feline immunodeficiency virus biologically and genetically different from the original U.S. isolate.
abstract: The Japanese isolate (TM1 strain) of feline immunodeficiency virus (FIV) which replicates in a feline CD4 (fCD4)-positive lymphoblastoid cell line (MYA-1 cells) was molecularly cloned from extrachromosomal closed circular DNA. The restriction map of the clone, termed pFTM 191 complete genome (CG), showed a considerable difference from that of the U.S. isolate (Petaluma strain) of FIV. The sequence homology in the long terminal repeat between the TM1 and Petaluma strain was 82%. The pFTM 191 CG was biologically active after transfection into Crandell feline kidney cells which were permissive for replication of FIV Petaluma. However, the progeny virions could not reinfect fCD4-negative Crandell feline kidney cells but could infect fCD4-positive MYA-1 cells. When a specific-pathogen-free cat was inoculated with the virus derived from the pFTM 191 CG, the cat seroconverted within 8 weeks postinoculation and FIV was reisolated at 4, 8, and 20 weeks postinoculation. These results indicate the infectivity of the pFTM 191 CG in vivo.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Miyazawa
authors.forename: M
authors.initials: M
authors.lastname: Fukasawa
authors.forename: A
authors.initials: A
authors.lastname: Hasegawa
authors.forename: N
authors.initials: N
authors.lastname: Maki
authors.forename: K
authors.initials: K
authors.lastname: Ikuta
authors.forename: E
authors.initials: E
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Hayami
authors.forename: T
authors.initials: T
authors.lastname: Mikami
mesh_terms: D000818:Animals; D001483:Base Sequence; D002415:Cats; D003001:Cloning, Molecular; D016089:Immunodeficiency Virus, Feline / Q000235:genetics* / Q000302:isolation & purification; D007564:Japan; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014481:United States; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins