Improved prenatal detection of fra(X)(q27.3): methods for prevention of false negatives in chorionic villus and amniotic fluid cell cultures.
The reliable detection of fra(X)(q27.3) in prenatal samples is important for providing genetic counseling. We have identified 5 new cases of prenatal fragile X [fra(X)] detection in 3 chorionic villus sample (CVS) and 2 amniotic fluid (AF) cell cultures. In 4 of the 5 cases, either excess thymidine (THY) or a combination of THY and 5-fluorodeoxyuridine (FUdR) was clearly superior to FUdR alone as fra(X) inducers. Amniocytes from one case were cultured only in RPMI-1640 and later exposed to FUdR or THY separately. They showed only 2% fra(X) while parallel cultures initiated in Chang medium and incubated in RPMI for at least 7 days (recovery) before fra(X) induction exhibited strikingly increased fra(X) frequencies. Chang medium alone will not allow fra(X) induction in AF (Jenkins EC, Brown WT [1986]: "Genetic Disorders and the Fetus: Diagnosis, Prevention and Treatment." New York: Plenum Press, pp 185-204). Now, using CVS cells, we report that only 1% and 0% fra(X) were detected using FUdR or THY in cells cultured in RPMI for 4 days after removal from Chang medium. Cells with 7 days "recovery" in RPMI exhibited increases from 2 to 6%. Therefore, we have found that Chang medium is very helpful when the appropriate recovery time in another medium is allowed before fra(X) induction. Some false negative reports can be attributed to: induction in Chang medium alone; lack of sufficient recovery time after initiating cells in Chang before induction; and unavailability of the excess THY fra(X) induction system.(ABSTRACT TRUNCATED AT 250 WORDS)
Jenkins
E C
EC
Department of Genetics, New York State Institute for Basic Research in Developmental Disabilities, Staten Island 10314.
Krawczun
M S
MS
Stark-Houck
S L
SL
Duncan
C J
CJ
Kunaporn
S
S
Gu
H
H
Schwartz-Richstein
C
C
Howard-Peebles
P N
PN
Gross
A
A
Sherman
S L
SL
D000649:Amniocentesis; D000653:Amniotic Fluid / Q000166:cytology; D002478:Cells, Cultured; D002824:Chorionic Villi / Q000648:ultrastructure; D015193:Chorionic Villi Sampling; D003470:Culture Media / Q000494:pharmacology; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005260:Female; D005467:Floxuridine / Q000494:pharmacology; D005600:Fragile X Syndrome / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D006801:Humans; D008297:Male; D011247:Pregnancy; D013936:Thymidine / Q000494:pharmacology; D014960:X Chromosome / Q000187:drug effects
D003470:Culture Media; D005467:Floxuridine; D013936:Thymidine