Single-strand breaks in DNA by 1-beta-D-arabinofuranosylcytosine and enhanced resistance frequency to N-phosphonoacetyl-L-aspartate.
1-beta-D-Arabinofuranosylcytosine (araC) caused concentration- and time--dependent single-strand breaks in SV28 cell DNA. Over the same concentration and time range, araC enhances the frequency of resistance to N-phosphonoacetyl-L-aspartate (PALA) and is also cytocidal (1). Comparison of induction of single-strand breaks by araC with its ability either to enhance the frequency of PALA-resistance or to kill cells, however, showed no obvious correlation, whether at different araC concentrations or exposure times. We conclude from these data that if there is a correlation between the ability of araC to cause single-strand breaks and either enhance PALA-resistance frequency or kill cells it is a complicated one or is masked by other events.
Chen
Y
Y
Department of Pharmacology, University of North Carolina, School of Medicine, Chapel Hill 27514.
Goz
B
B
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D002471:Cell Transformation, Neoplastic; D003561:Cytarabine / Q000494:pharmacology*; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000187:drug effects; D004351:Drug Resistance; D007700:Kinetics; D010746:Phosphonoacetic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D013539:Simian virus 40 / Q000235:genetics
D000964:Antimetabolites, Antineoplastic; D004277:DNA, Single-Stranded; D003561:Cytarabine; D001224:Aspartic Acid; C013195:sparfosic acid; D010746:Phosphonoacetic Acid