title: Antigenic analysis of the major human phosphoglucomutase isozymes: PGM1, PGM2, PGM3 and PGM4.
abstract: The cross-reactivity of human phosphoglucomutase isozymes (PGM1, PGM2, PGM3 and PGM4) has been investigated using anti-rabbit muscle PGM polyclonal antibodies. Significant differences were revealed: an IgG fraction of the antiserum reacted with the primary and secondary PGM1 isozymes of all the common phenotypes. However, there was no reaction with the PGM2 or PGM3 isozymes; thus these latter isozymes share no major antigenic determinants with human or rabbit PGM1 and are therefore structurally distinct. In contrast, the PGM isozymes of human milk attributed to a fourth locus, PGM4, showed similar cross-reactivity as PGM1 suggesting close structural similarity. The IgG was also employed as a reagent to remove PGM1 from haemolysates so as to allow the unambiguous assessment of the PGM2 isozyme patterns by isoelectric focusing. However, no proven genetic variation was encountered in a sample of 32 individuals.
authors.affiliation: MRC Human Biochemical Genetics Unit, Galton Laboratory, University College London, UK.
authors.forename: G A
authors.initials: GA
authors.lastname: Drago
authors.forename: D A
authors.initials: DA
authors.lastname: Hopkinson
authors.forename: S A
authors.initials: SA
authors.lastname: Westwood
authors.forename: D B
authors.initials: DB
authors.lastname: Whitehouse
mesh_terms: D003429:Cross Reactions; D004592:Electrophoresis, Starch Gel; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000276:immunology*; D008895:Milk, Human / Q000201:enzymology; D010733:Phosphoglucomutase / Q000276:immunology*; D010920:Placenta / Q000201:enzymology
chemical_list: D007527:Isoenzymes; D010733:Phosphoglucomutase