Respiration and mitochondrial ATPase in energized mitochondria during isoproterenol-induced cell injury of myocardium.
1. Respiration of mitochondria, membrane potential and mitochondrial ATPase under energized conditions were studied in rat myocardium during cell injury induced by treatment with isoproterenol. 2. Increase in the state 4 rate of respiration and ADP:O ratio, as well as decrease in the state 3 rate and Respiratory Control Ratio (RCR) were found. 3. The optimum pH for RCR and for maximum ATPase activity was shifted to lower values. 4. The state 3 respiration was more sensitive to oligomycin inhibition. 5. The mitochondria showed lower ability to generate membrane potential. 6. An increase in the K0.5 values for catalytic sites II and III of mitochondrial ATPase at pH 7.4 and 5.5 was found. 7. These results are consistent with alterations on the integrity of mitochondrial membrane, and corroborate with the hypothesis of changes on the mitochondrial ATPase during isoproterenol-induced cell injury of myocardium.
Uyemura
S A
SA
Departamento de Física e Química, Faculdade de Ciências Farmacêuticas-USP, Ribeirão Preto, S.P., Brasil.
Curti
C
C
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D006321:Heart / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D008564:Membrane Potentials; D008928:Mitochondria / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology* / Q000473:pathology / Q000648:ultrastructure; D010101:Oxygen Consumption; D051381:Rats; D011919:Rats, Inbred Strains
D000251:Adenosine Triphosphatases; D007545:Isoproterenol