Evidence for an active hydrophobic form of factor H that is able to induce secretion of interleukin 1-beta or by human monocytes.
We studied the capacity of human factor H to promote the secretion of a lymphocyte-activating factor (LAF) by human monocytes cultured under serum-free conditions. Presence of LAF in the culture supernatants was assessed with the mouse thymocyte assay. Highly purified factor H alone had no effect on thymocyte proliferation. When monocytes were cultured with factor H for 24 h, a significant secretion of LAF was observed. The effect was dose dependent over a range of factor H concentrations from 1 to 15 micrograms/ml. Polymyxin B did not abrogate the capacity of factor H to induce LAF secretion. Adsorption of factor H preparations onto anti-factor H-Sepharose completely suppressed the phenomenon. Conversely, the activity was recovered in the acidic eluate. Furthermore, factor H subpopulation phi 2, that was able to bind to phenyl-Sepharose, was a stronger inducer of LAF secretion by monocytes than the subpopulation phi 1 (which did not bind to phenyl-Sepharose). Using a specific radioimmunoassay for interleukin 1-beta (IL 1 beta), we observed a good correlation between the LAF activity and the amount of IL 1 beta secreted by human monocytes stimulated with factor H. We have shown previously that factor H (phi 2) bound specifically on Raji cells whereas factor H (phi 1) did not. These results argue for the participation of the interaction of factor H with its receptor to stimulate the secretion of IL 1 by monocytes and that the phi 2 form of factor H is a ligand for the human factor H receptor.
Iferroudjene
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D
INSERM Unité 78, Bois-Guillaume, France.
Schouft
M T
MT
Lemercier
C
C
Gilbert
D
D
Fontaine
M
M
D002478:Cells, Cultured; D003180:Complement C3b Inactivator Proteins / Q000737:chemistry / Q000494:pharmacology*; D017242:Complement Factor H; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism
C493495:CFH protein, human; D003180:Complement C3b Inactivator Proteins; D007375:Interleukin-1; D017242:Complement Factor H