Simian virus 5 membrane protein maturation: expression in virus-infected cells and from a eukaryotic vector.
Properties of the membrane protein (M) of the paramyxovirus simian virus 5 (SV5) isolated from purified SV5 virions, in SV5-infected cells or when expressed from cDNA using a eukaryotic vector (SV40-M) were examined. Kinetic (pulse-chase radiolabeling) studies showed that M protein expressed in SV5-infected and SV40-M recombinant virus-infected cells underwent maturation, detectable as time-dependent acquisition of reactivity with anti-M protein monoclonal antibodies. Kinetic studies using radiolabeled phosphate and studies with the alkylating agent N-ethylmaleimide indicated that the antigenic maturation of the M protein was not due to phosphorylation or disulfide bond formation, respectively. Immunofluorescent antibody staining studies showed a significant difference in staining patterns between SV40-M recombinant virus-infected cells and SV5-infected cells. SV40-M recombinant virus-infected cells exhibited an intensely staining cytoplasmic fibrillar network, whereas in SV5-infected cells, villar and some small granular structures were the only strongly staining structures.
Sheshberadaran
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Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208-3500.
Lamb
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RA
D000818:Animals; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D005822:Genetic Vectors; D007700:Kinetics; D010252:Paramyxoviridae / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism
D014759:Viral Envelope Proteins