title: Characterization of two genes, glpQ and ugpQ, encoding glycerophosphoryl diester phosphodiesterases of Escherichia coli.
abstract: The nucleotide sequences of the glpQ and ugpQ genes of Escherichia coli, which both encode glycerophosphoryl diester phosphodiesterases, were determined. The glpQ gene encodes a periplasmic enzyme of 333 amino acids, produced initially with a 25 residue long signal sequence, while ugpQ codes for a cytoplasmic protein of 247 amino acids. Despite differences in size and cellular location, significant similarity in the primary structures of the two enzymes was found suggesting a common evolutionary origin. The 3' end of the ugpQ gene overlaps an open reading frame that is transcribed in the opposite direction. This open reading frame encodes a polypeptide with an unusual composition, i.e., 46 of the 146 amino acids are Gln or Asn. This polypeptide and the UgpQ protein were identified in an in vitro transcription/translation system as proteins with apparent molecular weights of 19.5 and 27 kDa, respectively.
authors.affiliation: Department of Molecular Cell Biology, University of Utrecht, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Tommassen
authors.forename: K
authors.initials: K
authors.lastname: Eiglmeier
authors.forename: S T
authors.initials: ST
authors.lastname: Cole
authors.forename: P
authors.initials: P
authors.lastname: Overduin
authors.forename: T J
authors.initials: TJ
authors.lastname: Larson
authors.forename: W
authors.initials: W
authors.lastname: Boos
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010727:Phosphoric Diester Hydrolases / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D016415:Sequence Alignment
chemical_list: D004269:DNA, Bacterial; D010727:Phosphoric Diester Hydrolases; C038133:glycerophosphodiester phosphodiesterase