Cloning of xylanase gene of Streptomyces flavogriseus in Escherichia coli and bacteriophage lambda-induced lysis for the release of cloned enzyme.
The xylanase gene of Streptomyces flavogriseus was cloned in pUC8 plasmid and expressed in Escherichia coli lysogenic for lambda cI857. lambda-Induced lysis of E. coli at 42 degrees C allowed efficient release of cloned enzyme activity in extracellular environment. The xylanase gene was located in the 0.8-kb HindIII fragment and coded for 18,000 Mr xylanase.
Srivastava
R
R
Division of Microbial Genetics, Central Drug Research Institute, Lucknow, India.
Ali
S S
SS
Srivastava
B S
BS
D010582:Bacteriophage lambda / Q000235:genetics / Q000254:growth & development; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D043325:Xylan Endo-1,3-beta-Xylosidase
D004269:DNA, Bacterial; D006026:Glycoside Hydrolases; D043325:Xylan Endo-1,3-beta-Xylosidase