title: Beta-COP, a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to beta-adaptin.
abstract: We have cloned and sequenced beta-COP, a peripheral 110 kd Golgi membrane protein. beta-COP shows significant homology to beta-adaptin. It is present in a membrane-bound form and in a cytosolic complex of 13-14S, with a Stokes radius of approximately 10 nm and an estimated Mr of approximately 550,000. By immunofluorescence labeling, beta-COP is associated with the structures of the Golgi complex. Immunoelectron microscopy has localized beta-COP to non-clathrin-coated vesicles and cisternae of the Golgi complex. These coated vesicles accumulate in rat liver Golgi fractions treated with GTP gamma S and strongly label for beta-COP. Our data suggest that beta-COP is a component of a coat associated with vesicles and cisternae of the Golgi complex.
authors.affiliation: European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
authors.forename: R
authors.initials: R
authors.lastname: Duden
authors.forename: G
authors.initials: G
authors.lastname: Griffiths
authors.forename: R
authors.initials: R
authors.lastname: Frank
authors.forename: P
authors.initials: P
authors.lastname: Argos
authors.forename: T E
authors.initials: TE
authors.lastname: Kreis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D003001:Cloning, Molecular; D020756:Coatomer Protein; D004247:DNA / Q000235:genetics; D006056:Golgi Apparatus / Q000648:ultrastructure*; D006801:Humans; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000648:ultrastructure; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D051381:Rats; D015183:Restriction Mapping; D012995:Solubility; D014709:Vero Cells
chemical_list: D000911:Antibodies, Monoclonal; D020756:Coatomer Protein; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D004247:DNA