Regional localization of the hepatocyte growth factor (HGF) gene to human chromosome 7 band q21.1.
Hepatocyte growth factor (HGF) is a polypeptide involved in liver regeneration. Its amino acid sequence and gene structure are similar to those of coagulation-related serine proteases. We have used a cDNA clone of HGF and flow-sorted human chromosomes to assign this gene to chromosome 7. Fluorescence in situ hybridization of the HGF genomic clones to human metaphase chromosome spreads showed the localization of this gene to 7q21. Estimation of fluorescent signals relative to arbitrary reference points (ARPs) allowed further localization to 7q21.1.
Fukuyama
R
R
Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan.
Ichijoh
Y
Y
Minoshima
S
S
Kitamura
N
N
Shimizu
N
N
D002458:Cell Fractionation; D002460:Cell Line; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7; D005434:Flow Cytometry; D005819:Genetic Markers; D006133:Growth Substances / Q000235:genetics*; D017228:Hepatocyte Growth Factor; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007621:Karyotyping / Q000379:methods; D007834:Lasers; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D013234:Stem Cells
D005819:Genetic Markers; D006133:Growth Substances; D017228:Hepatocyte Growth Factor