Spinal cord projections of the rat main forelimb nerves, studied by transganglionic transport of WGA-HRP and by the disappearance of acid phosphatase.
The spinal cord projections of the 3 main forelimb nerves-median, radial and ulnar, were studied in the rat dorsal horn with transganglionic transport of wheat germ agglutinin-horseradish peroxidase (WGA-HRP), or using the disappearance of fluoride resistant acid phosphatase (FRAP) after nerve section. The projection patterns in lamina II were similar following the two procedures. The median and the radial nerve fibers projected to the medial and the intermediate thirds, respectively, of the dorsal horn lamina II in spinal cord segments C4-C8. The ulnar nerve projected to segments C6-C8 between the areas occupied by the other two nerves. The FRAP method also showed that the lateral part of lamina II, which was not filled by radial nerve fibers, received the projections from the dorsal cutaneous branches of cervical spinal nerves. In addition, FRAP disappeared from the medial end of segment T1 after skin incisions extending from the medial brachium to the axilla, which seemed due to severance of the cutaneous branchlets of the lateral anterior thoracic nerve. The FRAP procedure is thus sensitive enough to detect fibers in lamina II arising from small peripheral nerves, and may be used as an alternative to the anterograde tracing methods whenever there are no overlapping projections.
Castro-Lopes
J M
JM
Institute of Histology and Embryology, Faculty of Medicine, University of Oporto, Porto, Portugal.
Coimbra
A
A
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D005552:Forelimb / Q000294:innervation; D005724:Ganglia / Q000378:metabolism; D006735:Horseradish Peroxidase; D008297:Male; D008475:Median Nerve / Q000378:metabolism / Q000502:physiology*; D009420:Nervous System / Q000378:metabolism; D009424:Nervous System Physiological Phenomena; D011826:Radial Nerve / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000502:physiology*; D009435:Synaptic Transmission; D014459:Ulnar Nerve / Q000378:metabolism / Q000502:physiology*; D014909:Wheat Germ Agglutinins
D014909:Wheat Germ Agglutinins; D006735:Horseradish Peroxidase; D000135:Acid Phosphatase