title: Immunoelectron microscopic localization of the p21 protein in HT1080 human fibrosarcoma cell lines with altered N-ras gene expression.
abstract: The cellular localization of the p21 protein was studied by immunoelectron microscopy in human fibrosarcoma cells with different N-ras gene expression. The tumorigenic HT1080 cells--expressing equally the normal and mutant N-ras genes--contained the p21 in the plasma membrane, in pinocytotic areas as well as in the intracellular vesicles. The non-tumorigenic revertants--expressing more normal N-ras gene than the mutant one--contained p21 in the plasma membrane, especially at the cell junctions. These results suggest, that the mutant p21 may locate to the membrane areas connected with pino- or endocytotic functions.
authors.affiliation: 1st Institute of Pathology and Experimental Cancer Research, Semmelweis Medical University, Budapest, Hungary.
authors.forename: J
authors.initials: J
authors.lastname: Tímár
authors.forename: H
authors.initials: H
authors.lastname: Paterson
mesh_terms: D005354:Fibrosarcoma / Q000737:chemistry* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras; D006801:Humans; D016253:Microscopy, Immunoelectron; D016283:Proto-Oncogene Proteins p21(ras) / Q000032:analysis* / Q000502:physiology; D014407:Tumor Cells, Cultured
chemical_list: C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)