title: Human osteoblasts synthesize and respond to platelet-derived growth factor.
abstract: Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin. We previously demonstrated that PDGF is produced by osteogenic sarcoma cells. We report here that normal human bone-derived cells produce PDGF and that these cells have an osteoblastic phenotype. This was demonstrated by use of a double immunofluorescent technique and by examining cloned human adult osteoblasts. Northern blot analysis indicates that PDGF production is accounted for by expression of the PDGF-A gene. PDGF-AA and PDGF-BB generally stimulated thymidine incorporation in normal human bone explants to a similar extent. All of the cloned human osteoblasts responded to PDGF-BB while the response to PDGF-AA varied. Similarly, five cloned osteoblastic cell populations were shown to produce PDGF while one did not. This result supports the hypothesis that there are different osteoblastic cell populations that differ in their growth factor responses or in the production of growth factors. Our results suggest that PDGF-BB has the potential to act as a paracrine factor for normal human osteoblasts because all of the osteoblastic cell populations responded to PDGF-BB. None of the osteoblastic cell populations expressed the PDGF-B gene, indicating that it would not act as an autocrine factor. Although not definitive, our results suggest that PDGF-AA has the potential to act as an autocrine factor because osteoblastic bone cell populations were shown to express the PDGF-A gene and respond to PDGF-AA.
authors.affiliation: Department of Oral Biology, Boston University Medical Center, Massachusetts 02118.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: E
authors.initials: E
authors.lastname: Leeman
authors.forename: D C
authors.initials: DC
authors.lastname: Carnes
authors.forename: D T
authors.initials: DT
authors.lastname: Graves
mesh_terms: D015152:Blotting, Northern; D002999:Clone Cells; D005455:Fluorescent Antibody Technique; D006801:Humans; D007536:Isomerism; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000494:pharmacology; D011494:Protein Kinases / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor; D013936:Thymidine / Q000378:metabolism
chemical_list: D010982:Platelet-Derived Growth Factor; D011494:Protein Kinases; D017479:Receptors, Platelet-Derived Growth Factor; D013936:Thymidine