Maximizing sensitivity and specificity of PCR by pre-amplification heating.
D'Aquila
R T
RT
Infectious Disease Unit, Massachusetts General Hospital, Charlestown.
Bechtel
L J
LJ
Videler
J A
JA
Eron
J J
JJ
Gorczyca
P
P
Kaplan
J C
JC
D004587:Electrophoresis, Agar Gel; D015751:Genes, pol / Q000235:genetics; D015497:HIV-1 / Q000235:genetics; D006358:Hot Temperature; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity