Direct sequencing of the dopamine D2 receptor (DRD2) in schizophrenics reveals three polymorphisms but no structural change in the receptor.
The dopamine D2 receptor gene (gene symbol DRD2) is a candidate gene for schizophrenia because the potency of certain neuroleptics correlates with their affinity for this receptor. Seven regions of likely functional significance including the coding sequences and the splice junctions were fully sequenced in the dopamine D2 receptor of 14 schizophrenics (and partially in several others) meeting DSM-III-R diagnostic criteria and in four unaffected non-Caucasians (97 kb of total sequence). No structural changes were found, suggesting that alteration in the structure of the dopamine D2 receptor is not commonly involved in the etiology of schizophrenia. However, two common and one uncommon intragenic polymorphisms were found. At least one of the polymorphisms was informative for linkage in 70% of Caucasians and 78% of Koreans.
Sarkar
G
G
Department of Biochemistry and Molecular Biology, Mayo Clinic/Foundation, Rochester, Minnesota 55905.
Kapelner
S
S
Grandy
D K
DK
Marchionni
M
M
Civelli
O
O
Sobell
J
J
Heston
L
L
Sommer
S S
SS
D001483:Base Sequence; D004247:DNA; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011954:Receptors, Dopamine / Q000235:genetics*; D017448:Receptors, Dopamine D2; D012559:Schizophrenia / Q000235:genetics*
D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D004247:DNA