A fibroblast-derived tumor cytotoxic factor/F-TCF (hepatocyte growth factor/HGF) has multiple functions in vitro.
We previously demonstrated that a tumor cytotoxic factor(F-TCF) purified from the culture broth of human embryonic lung diploid fibroblast, IMR-90 cells was one of the human hepatocyte growth factors (hHGFs). In the present report, we demonstrate its biological functions. F-TCF showed moderate cytotoxicity on human tumor cell lines KB, BG-1, MCF-7 and Hs913 T, and strong cytotoxicity on mouse tumor cell lines Sarcoma 180, Meth A sarcoma and P388. On the contrary, F-TCF was a potent mitogen not only for adult rat hepatocytes, but also for human endothelial cells, HUVEC and human melanocytes. Moreover, F-TCF induced the differentiation of premyelocyte leukemia, HL-60 cells into morphologically granulocyte-like cells. These biological functions suggest that F-TCF is an effector molecule responsible for inflammation and repair in injured tissues including tumor and liver.
Shima
N
N
Research Institute of Life Science, Snow Brand Milk Products Co., Ltd., Tochigi, Japan.
Itagaki
Y
Y
Nagao
M
M
Yasuda
H
H
Morinaga
T
T
Higashio
K
K
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004727:Endothelium / Q000187:drug effects; D005347:Fibroblasts / Q000378:metabolism; D006133:Growth Substances / Q000302:isolation & purification / Q000494:pharmacology*; D017228:Hepatocyte Growth Factor; D006801:Humans; D007249:Inflammation / Q000473:pathology; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology; D008544:Melanocytes / Q000187:drug effects; D051379:Mice; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000473:pathology
D006133:Growth Substances; D017228:Hepatocyte Growth Factor