title: [Isolation, purification and characterization of a lectin from the seed of Erythrina costaricensis (Leguminosae)].
abstract: A lectin was isolated from the crude extract prepared from the seeds of E. costaricensis. It was purified by gel filtration on Sephadex G-100 followed by DEAE-Sephadex A-50 chromatography. PAGE revealed only one protein band, while analytical isoelectric focusing revealed four bands. The protein is a dimer with M(r) 58kda not united by disulfide bridges. It is a glycoprotein with 6.5% of neutral sugars, stable at 70 degrees C and at a pH range between 2 to 10. The protein exhibited a non specific agglutination of human erythrocytes, nevertheless it differentiated between erythrocytes of animal origin, agglutinating those of rabbit and chicken and not those from horse, goat, sheep or rat. Galactose, N-acetyl-D-galactosamine, lactose and EDTA are inhibitors while Ca++ and Mn++ acted as activators of the agglutination. No change in the blood pressure was observed when the lectin was intravenously injected in rats.
authors.affiliation: Departamento de Bioquímica, Escuela de Medicina, Universidad de Costa Rica.
authors.forename: C I
authors.initials: CI
authors.lastname: Nanne Echandi
authors.forename: F
authors.initials: F
authors.lastname: Aragón Ortiz
mesh_terms: D000818:Animals; D001789:Blood Group Antigens; D002645:Chickens; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004895:Erythrina / Q000737:chemistry*; D006384:Hemagglutination; D006801:Humans; D007525:Isoelectric Focusing; D037102:Lectins / Q000302:isolation & purification*; D037121:Plant Lectins; D010946:Plants, Medicinal; D011817:Rabbits; D051381:Rats
chemical_list: D001789:Blood Group Antigens; D037102:Lectins; D037121:Plant Lectins
vernacular_title: Aislamiento, purificación y caracterización de una lectina de la semilla del poró, Erythrina costaricensis (Leguminosae).