Platelet-derived growth factor is mitogenic for O-2Aadult progenitor cells.
We report that platelet-derived growth factor (PDGF) is a potent mitogen for oligodendrocyte type-2 astrocyte (O-2A) progenitor cells derived from the optic nerves of adult rats. Moreover, O-2Aadult progenitors cultured in PDGF express the range of properties we have described previously for O-2Aadult progenitors cultured in the presence of type-1 astrocytes. Similarly, previous studies have demonstrated that PDGF is able to mimic the influence of type-1 astrocytes on O-2Aperinatal progenitors. Specifically, O-2Aadult progenitors and O-2Aperinatal progenitors exposed to PDGF express differences in average cell cycle time (59 +/- 5 h for O-2Aadult progenitors versus 20 +/- 6 h for O-2Aperinatal progenitors), average rate of migration (4.1 +/- 0.6 microns h-1 versus 24.6 +/- 5.4 microns h-1), morphology (unipolar versus bipolar), and antigenic phenotype (04+ vimentin- versus 04- vimentin+). Thus, our present results indicate that a single signalling molecule secreted by type-1 astrocytes produces markedly different cellular behaviours in two related O-2A progenitor populations.
Wolswijk
G
G
Ludwig Institute for Cancer Research, London, England.
Riddle
P N
PN
Noble
M
M
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000276:immunology; D002455:Cell Division; D002465:Cell Movement; D003470:Culture Media; D008934:Mitogens / Q000494:pharmacology*; D009836:Oligodendroglia / Q000166:cytology / Q000187:drug effects*; D010641:Phenotype; D010982:Platelet-Derived Growth Factor / Q000276:immunology / Q000494:pharmacology*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D013997:Time Factors; D014746:Vimentin / Q000276:immunology
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000941:Antigens; D003470:Culture Media; D008934:Mitogens; D010982:Platelet-Derived Growth Factor; D014746:Vimentin