Human papillomavirus type 16 infection of the cervix: a comparison of differing DNA detection modes and the use of monoclonal antibodies against the major capsid protein.
An immuno-peroxidase technique using monoclonal antibodies against the major capsid protein L1 of HPV-16 was compared with dot-blot hybridisation of cervical scrapes and in situ hybridisation of cervical biopsy specimens for HPV-16 DNA. In a series of 20 patients all techniques were specific for HPV-16 infection. Ten patients were positive by dot-blot hybridisation and half of those were positive by in situ hybridisation. Only one of HPV-16 DNA positive cases showed L1 protein expression, apparently shortly after the onset of clinical infection. Whether major capsid protein expression reflects episodes of viral replication deserves further study.
Lacey
C J
CJ
Department of Genitourinary Medicine, General Infirmary, Leeds, UK.
Wells
M
M
Macdermott
R I
RI
Gibson
P E
PE
D000328:Adult; D000911:Antibodies, Monoclonal; D015139:Blotting, Southern; D002213:Capsid / Q000276:immunology; D036022:Capsid Proteins; D002584:Cervix Uteri / Q000382:microbiology; D015344:DNA Probes, HPV; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D009856:Oncogene Proteins, Viral / Q000276:immunology; D027383:Papillomaviridae; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000382:microbiology; D002577:Uterine Cervical Diseases / Q000175:diagnosis*
D000911:Antibodies, Monoclonal; D036022:Capsid Proteins; D015344:DNA Probes, HPV; D009856:Oncogene Proteins, Viral; C063317:L1 protein, Human papillomavirus type 16