Maximal activities of glutaminase, citrate synthase, hexokinase, 6-phosphofructokinase and lactate dehydrogenase in skin of immune-competent Balb/c and immune-deficient Balb/c (nu/nu) mice during wound healing.
1. The maximal activities of hexokinase (HK), 6-phosphofructokinase (PFK), lactate dehydrogenase, citrate synthase (CS) and glutaminase (GLU) which provide quantitative and qualitative indices of flux through several important metabolic pathways have been examined in the wounded skin of haired immune competent Balb/c mice and hairless immune deficient Balb/c (nu/nu) mice of various ages during the first ten days of wound healing. 2. The potential for glucose utilization and for aerobic metabolism as suggested by the maximal activities of HK, PFK, CS, were raised in the skin of Balb/c mice of various ages on all post wounding days. Increases in the maximal activity of GLU was observed only in the skin of 6 and 10 weeks old Balb/c mice during wound healing. 3. There was no evidence of a contribution to the maximal activity of GLU by infiltrating cells of the immune system to the wound site in the skin of either haired or hairless mice.
Nguyen
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Department of Microbiology, University of Western Australia, Queens Elizabeth II Medical Centre, Nedlands.
Keast
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D000375:Aging / Q000276:immunology; D000818:Animals; D002950:Citrate (si)-Synthase / Q000378:metabolism; D005972:Glutaminase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D007121:Immunocompetence; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010732:Phosphofructokinase-1 / Q000378:metabolism; D012867:Skin / Q000201:enzymology* / Q000276:immunology; D014945:Wound Healing / Q000276:immunology*
D007770:L-Lactate Dehydrogenase; D002950:Citrate (si)-Synthase; D006593:Hexokinase; D010732:Phosphofructokinase-1; D005972:Glutaminase