title: Primary structure and functional expression from complementary DNA of a brain calcium channel.
abstract: The primary structure of a voltage-dependent calcium channel from rabbit brain has been deduced by cloning and sequencing the complementary DNA. Calcium channel activity expressed from the cDNA is dramatically increased by coexpression of the alpha 2 and beta subunits, known to be associated with the dihydropyridine receptor. This channel is a high voltage-activated calcium channel that is insensitive both to nifedipine and to omega-conotoxin. We suggest that it is expressed predominantly in cerebellar Purkinje cells and granule cells.
authors.affiliation: Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Mori
authors.forename: T
authors.initials: T
authors.lastname: Friedrich
authors.forename: M S
authors.initials: MS
authors.lastname: Kim
authors.forename: A
authors.initials: A
authors.lastname: Mikami
authors.forename: J
authors.initials: J
authors.lastname: Nakai
authors.forename: P
authors.initials: P
authors.lastname: Ruth
authors.forename: E
authors.initials: E
authors.lastname: Bosse
authors.forename: F
authors.initials: F
authors.lastname: Hofmann
authors.forename: V
authors.initials: V
authors.lastname: Flockerzi
authors.forename: T
authors.initials: T
authors.lastname: Furuichi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D001921:Brain; D015220:Calcium Channels / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D008845:Microinjections; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000008:administration & dosage / Q000235:genetics; D011817:Rabbits; D014018:Tissue Distribution; D014981:Xenopus
chemical_list: D015220:Calcium Channels; D012333:RNA, Messenger; D004247:DNA