Production of endothelin-1 from the mesenteric arteries of streptozotocin-induced diabetic rats.
Release of endothelin-1 (ET-1) from the mesenteric arteries of Wistar rats with streptozotocin-induced diabetes (STZ-DM) rats and nondiabetic rats was measured by a specific enzyme immunoassay following purification using an immunoaffinity column. The mesenteric arteries from STZ-DM rats released a significantly higher amount of ET-1 as compared to control rats (35.8 +/- 2.8 vs 14.9 +/- 2.0 pg/1hr, p less than 0.05). The plasma level of ET-1 in STZ-DM rats was also elevated to a significant extent as compared to controls (5.1 +/- 0.4 vs 3.0 +/- 0.4 pg/ml, p less than 0.05). The systolic blood pressure of STZ-DM rats was significantly higher than of the controls (p less than 0.05). The increased level of plasma ET-1 as well as its release from the mesenteric artery of STZ-DM rats may suggest its release following damage to the endothelium caused by diabetes and/or by associated changes in blood pressure.
Takeda
Y
Y
Second Department of Internal Medicine, School of Medicine, Kanazawa University, Japan.
Miyamori
I
I
Yoneda
T
T
Takeda
R
R
D000818:Animals; D001794:Blood Pressure; D001835:Body Weight; D002846:Chromatography, Affinity; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D016232:Endothelins / Q000096:biosynthesis*; D007124:Immunoenzyme Techniques; D008297:Male; D008638:Mesenteric Arteries / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013311:Streptozocin / Q000494:pharmacology
D016232:Endothelins; D013311:Streptozocin