title: Retrovirus-derived shuttle vectors in the generation of murine bispecific MoAbs to be used in ex vivo and in vivo immunotherapy.
abstract: Mouse monoclonal antibodies specific for CD3, FcR and a melanoma associated antigen have been produced. Drug resistence of such hibrydomas has been obtained tranfecting them with plasmids containing genes conferring specific resistence. Retrovirus derived shuttle vectors with high transfection efficiency have been used for transfection. Hybrydomas were than fused and bispecific antibody producing cells selected. Purification was performed by HPLC. Such bispecific antibodies can be used in ex vivo and in vivo immunotherapy.
authors.affiliation: Dipartimento di Genetica, Biologia e Chimica Medica, Università degli Studi, Turin, Italy.
authors.forename: L B
authors.initials: LB
authors.lastname: Demonte
authors.forename: P
authors.initials: P
authors.lastname: Dellabona
authors.forename: P
authors.initials: P
authors.lastname: Nisticò
authors.forename: R
authors.initials: R
authors.lastname: Tecce
authors.forename: M
authors.initials: M
authors.lastname: Camagna
authors.forename: A
authors.initials: A
authors.lastname: Reyna
authors.forename: M
authors.initials: M
authors.lastname: Momo
authors.forename: P G
authors.initials: PG
authors.lastname: Natali
authors.forename: M
authors.initials: M
authors.lastname: Mariani
authors.forename: F
authors.initials: F
authors.lastname: Malavasi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D000951:Antigens, Neoplasm; D017252:CD3 Complex; D002459:Cell Fusion; D004351:Drug Resistance; D005822:Genetic Vectors; D006825:Hybridomas / Q000187:drug effects / Q000276:immunology; D007167:Immunotherapy; D058950:Melanoma-Specific Antigens; D051379:Mice; D009363:Neoplasm Proteins / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D011961:Receptors, Fc / Q000276:immunology; D012190:Retroviridae / Q000235:genetics*; D012641:Selection, Genetic
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; D017252:CD3 Complex; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D011948:Receptors, Antigen, T-Cell; D011961:Receptors, Fc