title: Structure-function relationship of hammerhead ribozymes as probed by 2'-modifications.
abstract: Hammerhead ribozymes containing 2'-fluoro- or 2'-aminonucleotides were prepared by automated chemical synthesis. Incorporation of 2'-fluorouridines, 2'-fluorocytidines or 2'-aminouridines did not appreciably decrease catalytic activity. The presence of 2'-aminocytidines, however, reduced the activity about 20-fold. No catalytic activity could be measured for ribozymes in which all adenosines were replaced by the 2'-fluoro analogue in presence of MgCl2. No single position could be found responsible for this loss of activity. In an attempt to construct ribozymes to hydrolyse HIV-RNA in the 5'-LTR region several constructs were tested on synthetic substrate as well as on run-off transcripts of about 1000 nucleotides length.
authors.affiliation: Max-Planck-Institut für Experimentelle Medizin, Göttingen, FRG.
authors.forename: W A
authors.initials: WA
authors.lastname: Pieken
authors.forename: D B
authors.initials: DB
authors.lastname: Olsen
authors.forename: H
authors.initials: H
authors.lastname: Aurup
authors.forename: D M
authors.initials: DM
authors.lastname: Williams
authors.forename: O
authors.initials: O
authors.lastname: Heidenreich
authors.forename: F
authors.initials: F
authors.lastname: Benseler
authors.forename: F
authors.initials: F
authors.lastname: Eckstein
mesh_terms: D000998:Antiviral Agents / Q000138:chemical synthesis / Q000494:pharmacology; D001483:Base Sequence; D006678:HIV / Q000187:drug effects; D016325:HIV Long Terminal Repeat; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000494:pharmacology; D016337:RNA, Catalytic / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D012367:RNA, Viral / Q000187:drug effects; D012265:Ribonucleotides
chemical_list: D000998:Antiviral Agents; D009843:Oligoribonucleotides; D016337:RNA, Catalytic; D012367:RNA, Viral; D012265:Ribonucleotides