Flow cytometric analysis of induced human graafian follicles. I. Demonstration and sorting of two luteinized cell populations.
OBJECTIVE
To test the hypothesis that induced human graafian follicles consist of different steroidogenic cell types on the basis of their light scatter characteristics as determined by flow cytometry.


METHODS
Cross-sectional, observational study.


METHODS
Flow cytometry laboratory.


METHODS
Thirty-six follicular aspirates from nine consecutive patients undergoing in vitro fertilization for tubal factor infertility were evaluated.


RESULTS
Two distinct luteal cell populations were recovered. Both populations were positive by Oil Red O staining, suggesting the presence of intracellular lipid. Neither population stained positively for the presence of HLe-1/CD45, an antigen present on all human leukocytes.


CONCLUSIONS
Cellular heterogeneity exists within the granulosa cell compartment.
Whitman
G F
GF
Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta 30912.
Boldt
J P
JP
Martinez
J E
JE
Pantazis
C G
CG
D015703:Antigens, CD / Q000032:analysis; D002469:Cell Separation; D003430:Cross-Sectional Studies; D005260:Female; D005434:Flow Cytometry; D006649:Histocompatibility Antigens / Q000032:analysis; D006801:Humans; D017493:Leukocyte Common Antigens; D008027:Light; D008055:Lipids / Q000032:analysis; D006080:Ovarian Follicle / Q000737:chemistry / Q000166:cytology*; D012542:Scattering, Radiation
D015703:Antigens, CD; D006649:Histocompatibility Antigens; D008055:Lipids; D017493:Leukocyte Common Antigens