[Plasmid pBR322 drug-resistance gene changes induced by glycidyl methacrylate].
A mutagen, glycidyl methacrylate (GMA), discovered by us a few years ago has been used to investigate the mutation mechanism of drug-resistance genes of plasmid pBR322. The results indicated that GMA binds strongly to pBR322 DNA, and this binding decreased the relative transformation efficiency using E. coli HB1O1 strain on LB plates containing ampicillin (Ap) or tetracycline (Tc). The mutants, ARpTSc, AspTRc and ASpTSc, have been isolated and their drug-resistance proved to be heritable.
Fang
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Institute of Basic Medical Sciences, Beijing.
D000668:Ampicillin Resistance / Q000235:genetics; D004852:Epoxy Compounds / Q000633:toxicity*; D008689:Methacrylates / Q000633:toxicity*; D009154:Mutation / Q000187:drug effects; D011815:R Factors / Q000187:drug effects*; D013753:Tetracycline Resistance / Q000235:genetics
D004852:Epoxy Compounds; D008689:Methacrylates; C007870:glycidyl methacrylate