title: Immunopathology of early graft-versus-host disease--a prospective study of skin, rectum, and peripheral blood in allogeneic and autologous bone marrow transplant recipients.
abstract: The immunopathological appearances of skin and rectum in 64 autologous and allogeneic recipients were determined before and after bone marrow transplantation. Patients who developed acute graft-versus-host disease were biopsied as soon as a clinical diagnosis was made. At the same time peripheral blood samples were collected for comparative analysis. Immunohistological and morphometric techniques were employed using a panel of monoclonal antibodies to T lymphocytes and subsets, B lymphocytes, natural killer cells, macrophages, and Langerhans cells. A reduction in the CD4/CD8 ratio after BMT was seen in skin and rectal biopsies from both autologous and allogeneic recipients with or without GVHD. The same pattern was observed in blood samples taken at the same time. Langerhans cells were reduced in the skin in all patients after BMT, probably by the conditioning regimen. Only a few cells expressing activation or natural killer cell markers were present and there were no changes observed in the macrophage population. This study has provided no evidence to implicate either CD4- or CD8-positive T lymphocytes as the initiators of the cellular damage in acute GVHD. The distribution of lymphocyte subsets in the blood was similar to that in the tissues, suggesting that the tissue changes reflect the pattern of lymphocyte repopulation after BMT and may have little bearing on the pathogenesis of GVHD.
authors.affiliation: Department of Pathology, Royal Victoria Infirmary, Newcastle upon Tyne, United Kingdom.
authors.forename: L
authors.initials: L
authors.lastname: Sviland
authors.forename: A D
authors.initials: AD
authors.lastname: Pearson
authors.forename: M A
authors.initials: MA
authors.lastname: Green
authors.forename: B D
authors.initials: BD
authors.lastname: Baker
authors.forename: E J
authors.initials: EJ
authors.lastname: Eastham
authors.forename: M M
authors.initials: MM
authors.lastname: Reid
authors.forename: P J
authors.initials: PJ
authors.lastname: Hamilton
authors.forename: S J
authors.initials: SJ
authors.lastname: Proctor
authors.forename: A J
authors.initials: AJ
authors.lastname: Malcolm
mesh_terms: D000293:Adolescent; D000328:Adult; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001706:Biopsy; D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D017252:CD3 Complex; D016516:CD4-CD8 Ratio; D016827:CD8 Antigens / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D006086:Graft vs Host Disease / Q000097:blood / Q000209:etiology / Q000473:pathology*; D006801:Humans; D007958:Leukocyte Count; D008875:Middle Aged; D011446:Prospective Studies; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D012007:Rectum / Q000473:pathology; D012867:Skin / Q000473:pathology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D016827:CD8 Antigens; D011948:Receptors, Antigen, T-Cell