Bone marrow-derived cultured mast cells and peritoneal mast cells as targets of a growth activity secreted by BALB/3T3 fibroblasts.
When fibroblast cell lines were cultured in contact with bone marrow-derived cultured mast cells (CMC), both NIH/3T3 and BALB/3T3 cell lines supported the proliferation of CMC. In contrast, when contact between fibroblasts and CMC was prohibited by Biopore membranes or soft agar, only BALB/3T3 fibroblasts supported CMC proliferation, suggesting that BALB/3T3 but not NIH/3T3 cells secreted a significant amount of a mast cell growth activity. Moreover, the BALB/3T3-derived growth activity induced the incorporation of [3H]thymidine by CMC and the clonal growth of peritoneal mast cells in methylcellulose. The mast cell growth activity appeared to be different from interleukin 3 (IL-3) and interleukin 4 (IL-4), because mRNAs for these interleukins were not detectable in BALB/3T3 fibroblasts. Although mast cells are genetically deficient in tissues of W/Wv mice, CMC did develop when bone marrow cells of W/Wv mice were cultured with pokeweed mitogen-stimulated spleen cell-conditioned medium. Because BALB/3T3 fibroblast-conditioned medium (BALB-FCM) did not induce the incorporation of [3H]thymidine by W/Wv CMC, the growth activity in BALB-FCM appeared to be a ligand for the receptor encoded by the W (c-kit) locus. Because CMC and peritoneal mast cells are obtained as homogeneous suspensions rather easily, these cells may be potentially useful as targets for the fibroblast-derived mast cell growth activity.
Jozaki
K
K
Department of Medicine, Osaka University Medical School, Japan.
Kuriu
A
A
Hirota
S
S
Onoue
H
H
Ebi
Y
Y
Adachi
S
S
Ma
J Y
JY
Tarui
S
S
Kitamura
Y
Y
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000648:ultrastructure; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media / Q000032:analysis / Q000494:pharmacology; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D006133:Growth Substances / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D008024:Ligands; D008407:Mast Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D010529:Peritoneal Cavity / Q000166:cytology*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D003470:Culture Media; D006133:Growth Substances; D008024:Ligands; D011956:Receptors, Cell Surface; D014316:Tritium; D013936:Thymidine