[The preparation of monoclonal antibody HI30-homoharringtonine liposomes (immunoliposomes) and targeting tests in vitro].
Liposomes encapsulating homoharringtonine were prepared using soybean phospholipids, egg yolk phospholipids and phospholipids from human red cell membrane, respectively. The quantity of homoharringtonine encapsulated by phospholipids from human red cell membrane was greater than that encapsulated by the other two types of liposomes, so this kind of phospholipid was used for further studies. The liposomes containing homoharringtonine (HH) were covalently coupled with HI30 (CD45). The mixture was separated by gel column chromatography. HI30-HH-liposomes demonstrated a sensitive targeting function with an immune activity equal to that observed with original HI30 in indirect immunofluorescence tests using the human cell line CEM-M3.
Chen
Z
Z
Institute of Hematology, Tianjin.
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000276:immunology; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D004910:Erythrocyte Membrane; D006248:Harringtonines / Q000008:administration & dosage / Q000494:pharmacology*; D006649:Histocompatibility Antigens / Q000276:immunology; D000077863:Homoharringtonine; D006801:Humans; D000922:Immunotoxins; D007938:Leukemia / Q000473:pathology*; D017493:Leukocyte Common Antigens; D008081:Liposomes; D010743:Phospholipids; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000972:Antineoplastic Agents, Phytogenic; D006248:Harringtonines; D006649:Histocompatibility Antigens; D000922:Immunotoxins; D008081:Liposomes; D010743:Phospholipids; D000077863:Homoharringtonine; D017493:Leukocyte Common Antigens