title: Thermospray liquid chromatography-mass spectrometry for the characterisation of sulphate ester conjugates.
abstract: Formation of polar conjugates is a well documented metabolic pathway for xenobiotics containing phenolic hydroxyl groups. This paper describes the analysis of two sulphate ester conjugates by fast atom bombardment mass spectrometry and thermospray liquid chromatography-mass spectrometry. Thermospray liquid chromatography-mass spectrometry proved the more successful technique for obtaining the molecular weight of the intact conjugate, but only by removal of the buffer from the high-performance liquid chromatography eluent.
authors.affiliation: Department of Biochemical Pharmacology, Glaxo Group Research Limited, Hertfordshire, UK.
authors.forename: G D
authors.initials: GD
authors.lastname: Bowers
authors.forename: D M
authors.initials: DM
authors.lastname: Higton
authors.forename: G R
authors.initials: GR
authors.lastname: Manchee
authors.forename: J
authors.initials: J
authors.lastname: Oxford
authors.forename: D A
authors.initials: DA
authors.lastname: Saynor
mesh_terms: D000420:Albuterol / Q000378:metabolism* / Q000652:urine; D000818:Animals; D000932:Antiemetics / Q000032:analysis / Q000378:metabolism*; D001646:Bile / Q000737:chemistry; D002144:Callithrix; D002853:Chromatography, Liquid / Q000379:methods*; D004285:Dogs; D004952:Esters / Q000032:analysis* / Q000378:metabolism; D007093:Imidazoles / Q000032:analysis / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D017294:Ondansetron; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013431:Sulfates / Q000032:analysis* / Q000378:metabolism
chemical_list: D000932:Antiemetics; D004952:Esters; D007093:Imidazoles; D013431:Sulfates; D017294:Ondansetron; D000420:Albuterol