title: Chronic morphine treatment selectively suppresses macrophage colony formation in bone marrow.
abstract: Opioids have been shown to have diverse effects on the immune system, both in vivo and in vitro, but their interactions on immature progenitor cells have been little studied. We have examined the effects of chronic morphine treatment of mice on colony formation by bone marrow cells in vitro. Bone marrow cells from mice implanted with morphine pellets for 72 h showed a 65% decrease in their response to macrophage colony stimulating factor (M-CSF). In contrast, chronic morphine treatment had no effect on the response of bone marrow cells to granulocyte/macrophage colony stimulating factor (GM-CSF). Removal of the morphine pellets from the mice resulted in a time-dependent reversal of the inhibition of macrophage colony formation, and the inhibition was completely blocked by simultaneous administration of naloxone and morphine pellets to the mice. No inhibition of colony formation was observed in bone marrow cells from mice treated with a single acute dose of morphine. Incubation of bone marrow cells from untreated mice for 7 days with in vitro morphine concentrations as low as 25 microM also reduced macrophage colony formation, and the opioid peptide beta-endorphin was even more potent, significantly reducing macrophage colony formation at concentrations as low as 0.25 microM. In agreement with the in vivo effects, neither opioid in vitro had a significant effect on granulocyte/macrophage colony formation. These results suggest that opioids may significantly alter the maturation of immune cells, which could result in potent effects on overall immune competence.
authors.affiliation: Department of Pharmacology, University of Minnesota Medical School, Minneapolis 55455.
authors.forename: S
authors.initials: S
authors.lastname: Roy
authors.forename: S
authors.initials: S
authors.lastname: Ramakrishnan
authors.forename: H H
authors.initials: HH
authors.lastname: Loh
authors.forename: N M
authors.initials: NM
authors.lastname: Lee
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D003114:Colony-Forming Units Assay; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D009020:Morphine / Q000494:pharmacology*; D009270:Naloxone / Q000494:pharmacology; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D013234:Stem Cells / Q000187:drug effects; D001615:beta-Endorphin / Q000494:pharmacology
chemical_list: D009270:Naloxone; D001615:beta-Endorphin; D009020:Morphine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor