Identification of lymphocyte subpopulations by indirect immunofluorescence--a micromethod.
The microtechnique described uses for the immunofluorescent determination of lymphocyte surface markers 96-well microplates instead of traditional test-tubes. The article covers in detail all steps of the laboratory procedure--the isolation of lymphocytes, staining of the cells and their preparation for microscopic evaluation. The main contributions of the micromethod (reduction of costs and labour) are characterized in the discussion.
Petrek
M
M
Dept. of Microbiology and Immunology, Medical Faculty UP, Olomouc, Czechoslovakia.
D005455:Fluorescent Antibody Technique; D006801:Humans; D016131:Lymphocyte Subsets