Activation of phospholipase D in rabbit neutrophils by fMet-Leu-Phe is mediated by a pertussis toxin-sensitive GTP-binding protein that may be distinct from a phospholipase C-regulating protein.
Stimulation by N-formyl-Met-Leu-Phe (fMLP) of rabbit peritoneal neutrophils, in which phosphatidylcholine was preferentially labeled with 1-O-[3H]octadecyl lyso platelet-activating factor, activated phospholipase D, resulting in the formation of [3H]PA from [3H]PC. A direct activator of GTP-binding proteins (G-proteins), NaF, also stimulated [3H]PA formation. fMLP-stimulated [3H]PA formation was inhibited by pertussis toxin (IAP) in a time- and dose-dependent manner. IAP also inhibited fMLP-stimulated IP3 formation, but the inhibition of IP3 formation was significantly greater than that of [3H]PA formation. These results indicate that activation of phospholipase D by fMLP in rabbit neutrophils is mediated by an IAP-sensitive G-protein that may be distinct from a phospholipase C-regulating protein.
Kanaho
Y
Y
Department of Biochemistry, Gifu University School of Medicine, Japan.
Kanoh
H
H
Nozawa
Y
Y
D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D019204:GTP-Binding Proteins / Q000502:physiology*; D020404:Glycerophospholipids; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000201:enzymology*; D037342:Pertussis Toxin; D010712:Phosphatidic Acids / Q000378:metabolism; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010739:Phospholipase D / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011817:Rabbits; D012969:Sodium Fluoride / Q000494:pharmacology; D010738:Type C Phospholipases / Q000502:physiology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D020404:Glycerophospholipids; D010712:Phosphatidic Acids; D010566:Virulence Factors, Bordetella; C051521:phosphatidylethanol; D009240:N-Formylmethionine Leucyl-Phenylalanine; D012969:Sodium Fluoride; D037342:Pertussis Toxin; D010727:Phosphoric Diester Hydrolases; D010738:Type C Phospholipases; D010739:Phospholipase D; D019204:GTP-Binding Proteins; D043265:Phosphatidylinositol Diacylglycerol-Lyase