Distinctive molecular markers and biological activities in two tumour-specific murine T suppressor factors.
Murine suppressor factors (SF) in tumour-bearer serum (TBS) and tumour-bearer spleen cell culture supernatants (SCCS) were compared with regard to their molecular markers, cellular requirements and suppressive activities. Suppressor factors in SCCS bear the idiotype I-J, and markers recognized by B16G and anti-lipomodulin antibodies require Ly2+ I-J+ cells for their production and cells of the same phenotypes, for suppression to be manifested in leucocyte adherence inhibition (LAI) assays, are not immunoglobulin (Igh)-restricted and are active in both the afferent and efferent phases of the immune response. Suppressor factors from TBS resemble those from SCCS with regard to idiotype, I-J and lipomodulin-like markers, but in contrast, do not bind to B16G antibody, require Ly2+ but not I-J+ cells for suppression to be manifested in LAI assays, are Igh-restricted, and suppress delayed-type hypersensitivity (DTH) reactions only when injected in the afferent phase. These results show that the SF from the different sources in tumour-bearing mice are not identical; they may be different parts of a suppressor cascade, or they may belong to entirely separate suppressor cascades.
Greer
J M
JM
Microbiology Department, University of Queensland, Australia.
Halliday
W J
WJ
D000818:Animals; D017302:Annexins; D000911:Antibodies, Monoclonal / Q000276:immunology; D000912:Antibodies, Neoplasm / Q000276:immunology*; D002135:Calcium-Binding Proteins / Q000276:immunology; D002295:Carcinoma, Transitional Cell / Q000276:immunology; D005260:Female; D005354:Fibrosarcoma / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006968:Hypersensitivity, Delayed; D007279:Injections, Subcutaneous; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen; D013491:Suppressor Factors, Immunologic / Q000737:chemistry / Q000502:physiology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014407:Tumor Cells, Cultured
D017302:Annexins; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D002135:Calcium-Binding Proteins; D006023:Glycoproteins; D000949:Histocompatibility Antigens Class II; C043911:I-J-antigen; D013491:Suppressor Factors, Immunologic; C030958:lipomodulin