Arrest of MCF-7 cell migration by laminin in vitro: possible mechanisms.
Laminin, a major basement membrane component, arrested the migration of MCF-7/AZ human breast adenocarcinoma cells that were not invasive in vitro. Migration of invasive MCF-7/6 cells was not affected by laminin. Both cell types expressed the 67 kD laminin receptor, at both mRNA and protein level, but did not express the alpha 6 subunit of the VLA-6 integrin-type laminin receptor. The presence of YIGSR peptides (100 micrograms/ml), reported to block the interaction between laminin and its 67 kD receptor, did not change the migratory response of MCF-7/AZ or MCF-7/6 cells when meeting laminin lanes. In addition, the migration of these cell types was not affected by the presence of 17-beta-estradiol (10(-6) M) or all-trans retinoic acid (10(-6) M), which were both reported to increase the number of 67 kD receptors. We could therefore not assign an involvement of the 67 kD receptors in migration of MCF-7 cells on laminin, nor did we find evidence that conditioned medium of MCF-7/6 cells contains factors that are able to initiate migration of MCF-7/AZ cells on laminin.
Coopman
P
P
Department of Radiotherapy and Nuclear Medicine, University Hospital, Gent, Belgium.
Verhasselt
B
B
Bracke
M
M
De Bruyne
G
G
Castronovo
V
V
Sobel
M
M
Foidart
J M
JM
Van Roy
F
F
Mareel
M
M
D000230:Adenocarcinoma / Q000737:chemistry / Q000503:physiopathology*; D001943:Breast Neoplasms / Q000737:chemistry / Q000503:physiopathology*; D002465:Cell Movement / Q000187:drug effects; D003470:Culture Media; D006801:Humans; D007797:Laminin / Q000494:pharmacology*; D011946:Receptors, Antigen / Q000032:analysis; D011971:Receptors, Immunologic / Q000032:analysis / Q000378:metabolism; D017474:Receptors, Laminin; D014407:Tumor Cells, Cultured / Q000187:drug effects
D003470:Culture Media; D007797:Laminin; D011946:Receptors, Antigen; D011971:Receptors, Immunologic; D017474:Receptors, Laminin