Effect of 2% dimethyl sulfoxide on the mitogenic properties of epidermal growth factor and hepatocyte growth factor in primary hepatocyte culture.
Two percent dimethyl sulfoxide (DMSO) reversibly inhibited DNA synthesis in primary rat hepatocyte cultures maintained with epidermal growth factor (EGF) or hepatocyte growth factor (HGF). These data suggest that, in vitro, DMSO is a non-specific inhibitor of hepatocyte proliferation, regardless of the stimulating mitogen. In addition, removal of DMSO from mitogen-free cultures resulted in an increase in DNA synthesis. Protein synthesis gradually but irreversibly declined in all cultures after DMSO removal. The relevance of these findings to regulation of hepatocyte growth is discussed.
Kost
D P
DP
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710.
Michalopoulos
G K
GK
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000037:antagonists & inhibitors* / Q000502:physiology; D006133:Growth Substances / Q000502:physiology*; D017228:Hepatocyte Growth Factor; D007700:Kinetics; D008099:Liver / Q000166:cytology; D008297:Male; D014176:Protein Biosynthesis; D051381:Rats; D011916:Rats, Inbred F344
D006133:Growth Substances; D004815:Epidermal Growth Factor; D017228:Hepatocyte Growth Factor; D004247:DNA; D004121:Dimethyl Sulfoxide