title: Two 68-kDa proteins in slow axonal transport belong to the 70-kDa heat shock protein family and the annexin family.
abstract: The major 68-kDa protein found selectively in the faster of the two subcomponents of slow axonal transport [group IV or slow component b (SCb)] in the rat sciatic nerve has been characterized. It was found to contain two distinct classes of proteins, S1 and S2, both of which have isoelectric points of 5.7, but differ in their solubility in the presence of calcium. The S1 protein, which contributes up to 70% of the 68-kDa component, was soluble in the presence or absence of calcium, whereas the S2 protein was bound to the cytoskeleton in a calcium-dependent manner. Further characterization of the two proteins by peptide mapping and immunological methods revealed that the S1 protein belonged to a family of proteins related to the 70-kDa heat shock protein, whereas the S2 protein was identical to 68-kDa calelectrin (annexin VI). Selective occurrence in SCb of these proteins with potential abilities to regulate protein-protein or protein-membrane interactions suggests that they may play important roles in the control of cytoskeletal organization in the axon, because SCb contains mainly cytoskeletal proteins in a more dynamic form compared with the slowest rate component, slow component a, which is enriched in the stably polymerized form of these proteins.
authors.affiliation: Department of Molecular and Cellular Neurobiology, Gunma University School of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Sekimoto
authors.forename: T
authors.initials: T
authors.lastname: Tashiro
authors.forename: Y
authors.initials: Y
authors.lastname: Komiya
mesh_terms: D000818:Animals; D017302:Annexins; D001369:Axons / Q000378:metabolism*; D001692:Biological Transport; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000737:chemistry*; D008297:Male; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D017302:Annexins; D002135:Calcium-Binding Proteins; D006360:Heat-Shock Proteins; D009419:Nerve Tissue Proteins