T cell receptor delta diversity of freshly isolated T lymphocytes in rheumatoid synovitis.
In some patients with rheumatoid synovitis the distribution of V delta gene families used by freshly isolated T lymphocytes from the synovial fluid and peripheral blood is different: peripheral blood T lymphocytes preferentially use the V delta 2 gene segment, whereas the majority of synovial fluid T lymphocytes use the V delta 1. The antigenic diversity of T cell receptor gamma/delta is mainly dependent upon base sequences in the V-J junction. In the present study we investigated whether the synovial fluid T lymphocytes expressing the V delta 1 gene segment were derived from a polyclonal or monoclonal expansion. The sequences encoding the V-J junctions of 11 cDNA clones derived from the synovium of two patients with chronic arthritis were determined. Our data demonstrate that the V delta 1 cDNA clones have different V-J junction sequences. This indicates that the V delta 1-expressing T cells found in the synovial compartment are polyclonally expanded.
Sioud
M
M
Institute of Immunology and Rheumatology, University of Oslo, Norway.
Førre
O
O
Natvig
J B
JB
D001171:Arthritis, Juvenile / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015329:Gene Rearrangement, T-Lymphocyte; D006801:Humans; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D016692:Receptors, Antigen, T-Cell, gamma-delta; D013585:Synovitis / Q000276:immunology*; D013601:T-Lymphocytes / Q000502:physiology*
D009841:Oligonucleotides; D011948:Receptors, Antigen, T-Cell; D016692:Receptors, Antigen, T-Cell, gamma-delta; D004247:DNA