Quantification of acitretin in human plasma by microbore liquid chromatography-negative chemical ionization mass spectrometry.
A highly sensitive liquid chromatographic-mass spectrometric procedure has been developed to quantitate plasma concentrations of acitretin, a dermatologic agent used to treat severe psoriasis. The assay utilizes the combination of normal-phase microbore high-performance liquid chromatography, negative chemical ionization mass spectrometry, selective ion monitoring and stable isotope dilution. The method has been used to measure acitretin and its metabolite, 13-cis-acitretin, over a range of 1-20 ng/ml in human plasma. The inter-assay precision was 5.3% for acitretin and 3.9% for 13-cis-acitretin, while the intra-assay precisions for acitretin and 13-cis-acitretin were 10.8 and 12.7%, respectively. Reproducibility of the assay for acitretin and 13-cis-acitretin, which was determined by the relative standard deviation of multiple analyses of the same quality assurance sample, was 5.9 and 8.1%, respectively.
Fayer
B E
BE
Department of Drug Metabolism, Hoffmann-La Roche, Inc., Nutley, NJ 07110.
Huselton
C A
CA
Garland
W A
WA
Liberato
D J
DJ
D017255:Acitretin; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D013997:Time Factors; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000097:blood
D014212:Tretinoin; D017255:Acitretin