Immunohistochemical localization of MAM-3 and MAM-6 antigens in adenoid cystic carcinoma.
MAM-3 and MAM-6 antigens were detected immunohistochemically in 34 cases of adenoid cystic carcinomas (ACC) of the salivary glands and these patterns were compared to these of epithelial membrane antigen (EMA) and laminin. ACC was histologically divided into three types; the cribriform pattern, the tubular and trabecular pattern, and the solid cluster pattern. Immunostaining of EMA and MAM-6 antigen had a similar distributions in the luminal borders of luminal tumor cells, whereas the MAM-3 antigen was slight or negative in luminal borders. Myoepithelial derived tumor cells of ACC accompanying hyaline stroma demonstrated positive staining for the MAM-6 antigen (whole cell positive type), and luminal tumor cells of microcysts showed strong staining for the MAM-3 antigen. Laminin staining was confined to the basement membrane and surface borders in pseudocyst cavities. In salivary gland ACC, laminin staining can be used as a marker of pseudocyst surfaces and immunostaining of EMA and the MAM-6 antigen as a marker of luminal borders of cyst. These two histochemical markers were useful for discriminating pseudocyst and cyst.
Yamada
K
K
Department of Oral Surgery, Asahi University School of Dentistry, Gifu, Japan.
Kunikata
M
M
Mori
M
M
Chomette
G
G
Auriol
M
M
Vaillant
J M
JM
Tubura
A
A
Morii
S
S
Hilgers
J
J
D000951:Antigens, Neoplasm / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D003528:Carcinoma, Adenoid Cystic / Q000737:chemistry* / Q000473:pathology; D004848:Epithelium / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D007797:Laminin; D008562:Membrane Glycoproteins / Q000032:analysis*; D018396:Mucin-1; D012468:Salivary Gland Neoplasms / Q000737:chemistry* / Q000473:pathology
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D007797:Laminin; D008562:Membrane Glycoproteins; D018396:Mucin-1