A genomic sequence of the Schizosaccharomyces pombe 16 kDa vacuolar H(+)-ATPase.
We have isolated the gene encoding the 16 kDa vacuolar H(+)-ATPase from Schizosaccharomyces pombe. On the basis of RNA splicing signals and amino acid sequence homology with other 16 kDa H(+)-ATPases, the genomic DNA sequence indicated the 16 kDa protein is encoded by five exons. The C-terminal 50 amino acids has more than 90% homology with vacuolar H(+)-ATPases of mammalian cells.
Toyama
R
R
Laboratory of Molecular Virology, National Cancer Institute, Bethesda, MD 20892.
Goldstein
D J
DJ
Schlegel
R
R
Dhar
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry*; D005091:Exons; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000235:genetics*; D012326:RNA Splicing; D012331:RNA, Fungal / Q000502:physiology; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics* / Q000648:ultrastructure; D014617:Vacuoles / Q000201:enzymology
D004271:DNA, Fungal; D012331:RNA, Fungal; D006180:Proton-Translocating ATPases