title: Abortifacient property of bovine herpesvirus type 1 isolates that represent three subtypes determined by restriction endonuclease analysis of viral DNA.
abstract: Bovine herpesvirus type 1 (BHV-1) isolates are classified into 3 subtypes by use of restriction endonuclease analysis. Isolates from aborted fetuses have been either subtype 1 or 2a, whereas subtype 2b viruses have not been associated with abortion. We assessed the abortifacient property of isolates representing each of the 3 BHV-1 subtypes by IV inoculation of heifers with the virus 25 to 27 weeks after breeding. Three heifers were given Cooper (subtype 1) isolate, 3 heifers were given FI (subtype 2a) isolate, and 5 heifers were given K22 (subtype 2b) isolate. All heifers developed fever and viremia 2 to 5 days after inoculation. Heifers given Cooper or FI isolate aborted between 17 and 85 days after inoculation. The 5 heifers given K22 isolate delivered full-term calves. Placenta was obtained from 4 of the 5 heifers, and K22 virus was isolated from each placenta. Four calves had BHV-1 neutralizing antibody in precolostral serum, with titer ranging from 1:4 to 1:512.
authors.affiliation: USDA Agricultural Research Service, National Animal Disease Center, Ames, IA 50010.
authors.forename: J M
authors.initials: JM
authors.lastname: Miller
authors.forename: C A
authors.initials: CA
authors.lastname: Whetstone
authors.forename: M J
authors.initials: MJ
authors.lastname: Van der Maaten
mesh_terms: D000034:Abortion, Veterinary / Q000382:microbiology*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006566:Herpesviridae Infections / Q000382:microbiology / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine / Q000145:classification* / Q000235:genetics; D007150:Immunohistochemistry; D009296:Nasal Cavity / Q000382:microbiology; D010920:Placenta / Q000382:microbiology; D011247:Pregnancy; D015183:Restriction Mapping; D014621:Vagina / Q000382:microbiology; D014766:Viremia / Q000382:microbiology / Q000662:veterinary
chemical_list: D004279:DNA, Viral