Molecular cloning and nucleotide sequence of the Rhizobium phaseoli recA gene.
A recombinant lambda phage carrying the recA gene of Rhizobium phaseoli was isolated from a R. phaseoli genomic library by complementation of the Fec- phenotype of the recombinant phage in Escherichia coli. When expressed in E. coli, the cloned recA gene was shown to restore resistance to both UV irradiation and the DNA alkylating agent methyl methanesulphonate (MMS). The R. phaseoli recA gene also promoted homologous recombination in E. coli. The cloned recA gene was only weakly inducible in E. coli recA strains by DNA damaging agents. The DNA sequence of the R. phaseoli recA gene was determined and compared with published recA sequences. No LexA-binding site was detected in the R. phaseoli recA upstream region.
Michiels
J
J
F.A. Janssens Laboratory of Genetics, University of Leuven, Heverlee, Belgium.
Vande Broek
A
A
Vanderleyden
J
J
D000595:Amino Acid Sequence; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005814:Genes, Viral; D008969:Molecular Sequence Data; D012231:Rhizobium / Q000235:genetics* / Q000528:radiation effects; D012689:Sequence Homology, Nucleic Acid; D014466:Ultraviolet Rays