Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family.
A new human cyclin, named cyclin E, was isolated by complementation of a triple cln deletion in S. cerevisiae. Cyclin E showed genetic interactions with the CDC28 gene, suggesting that it functioned at START by interacting with the CDC28 protein. Two human genes were identified that could interact with cyclin E to perform START in yeast containing a cdc28 mutation. One was CDC2-HS, and the second was the human homolog of Xenopus CDK2. Cyclin E produced in E. coli bound and activated the CDC2 protein in extracts from human G1 cells, and antibodies against cyclin E immunoprecipitated a histone H1 kinase from HeLa cells. The interactions between cyclin E and CDC2, or CDK2, may be important at the G1 to S transition in human cells.
Koff
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Department of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Cross
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Fisher
A
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Schumacher
J
J
Leguellec
K
K
Philippe
M
M
Roberts
J M
JM
D000595:Amino Acid Sequence; D001483:Base Sequence; D016203:CDC2 Protein Kinase / Q000502:physiology*; D002453:Cell Cycle; D016213:Cyclins / Q000145:classification / Q000502:physiology*; D004247:DNA / Q000235:genetics; D004789:Enzyme Activation; D005816:Genetic Complementation Test; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016415:Sequence Alignment
D016213:Cyclins; D012333:RNA, Messenger; D004247:DNA; D016203:CDC2 Protein Kinase