Transformation-defective v-ski induces MyoD and myogenin expression but not myotube formation.
The ski oncogene induces muscle differentiation in otherwise nonmyogenic quail embryo cells (C. Colmenares and E. Stavnezer, Cell 59:293-303, 1989). Here we report that v-ski induces both MyoD and myogenin expression, suggesting that activation of these muscle regulatory genes may be a critical step in ski-induced myogenesis. We also describe a transformation-defective mutant of v-ski (tdM5i) that fails to induce myotube formation, although it induces the expression of many muscle-specific genes, including the MyoD and myogenin genes. Therefore, if activation of MyoD and myogenin expression is a necessary component of the myogenic program triggered by ski, it is clearly insufficient to account for complete muscle differentiation.
Colmenares
C
C
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati Medical Center, Ohio 45267-0524.
Teumer
J K
JK
Stavnezer
E
E
D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics; D004625:Embryo, Nonmammalian; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D009132:Muscles / Q000166:cytology* / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D017570:MyoD Protein; D018008:Myogenin; D009857:Oncogenes; D011784:Quail; D015534:Trans-Activators / Q000235:genetics
D004268:DNA-Binding Proteins; D009124:Muscle Proteins; D017570:MyoD Protein; D018008:Myogenin; D015534:Trans-Activators