Drosophila alcohol dehydrogenase: a novel reporter gene for use in mammalian embryos.
A reporter gene construct containing the Drosophila alcohol dehydrogenase (ADH) gene under regulation of the Rous sarcoma virus long terminal repeat (RSV LTR) was microinjected into mouse zygote pronuclei. ADH activity after injection of the RSV-ADH construct was visualized in cultured embryos by means of a simple histochemical enzyme assay. The RSV LTR was an efficient promoter that led to abundant ADH activity at all stages of preimplantation development. There was a high incidence of mosaicism among stained embryos.
Nielsen
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Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143.
Pedersen
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D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics; D001755:Blastocyst / Q000201:enzymology*; D002467:Cell Nucleus; D046508:Culture Techniques; D004279:DNA, Viral; D004330:Drosophila; D004626:Embryology / Q000379:methods; D005819:Genetic Markers; D051379:Mice; D008845:Microinjections; D012091:Repetitive Sequences, Nucleic Acid
D004279:DNA, Viral; D005819:Genetic Markers; D000426:Alcohol Dehydrogenase