Bone marrow from children in relapse with pre-B acute lymphoblastic leukemia proliferates and disseminates rapidly in scid mice.
Bone marrow samples from patients with pre-B acute lymphoblastic leukemia (pre-B ALL), either at diagnosis or at relapse, were transplanted into scid mice to determine whether these freshly obtained leukemic cells could proliferate in vivo and whether there were any differences in their in vivo growth characteristics. Cells from three patients who relapsed within 13 months of diagnosis proliferated rapidly in the murine bone marrow, spleen, and thymus, invaded peripheral organs, and resulted in morbidity and mortality of the animals within 4 to 16 weeks. Cells from two patients who relapsed 3.5 years after diagnosis grew much slower than the early relapse samples, taking up to 30 weeks to infiltrate the bone marrow of recipient mice. In contrast, leukemic cells were absent or were detected at low numbers in scid mice transplanted with cells obtained at diagnosis from three patients who have not yet relapsed. These results show an increased ability of leukemic cells from patients with aggressive lymphoblastic leukemia of poor prognosis to proliferate in scid mice.
Kamel-Reid
S
S
Department of Genetics and Pathology, Hospital for Sick Children, Toronto, Ontario, Canada.
Letarte
M
M
Doedens
M
M
Greaves
A
A
Murdoch
B
B
Grunberger
T
T
Lapidot
T
T
Thorner
P
P
Freedman
M H
MH
Phillips
R A
RA
D000293:Adolescent; D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D001853:Bone Marrow / Q000473:pathology*; D001921:Brain / Q000473:pathology; D002455:Cell Division; D002648:Child; D002675:Child, Preschool; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007223:Infant; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D015260:Neprilysin; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology*
D000943:Antigens, Differentiation; D000951:Antigens, Neoplasm; D006684:HLA-DR Antigens; D015260:Neprilysin