title: Analysis of equine humoral immune responses to the transmembrane envelope glycoprotein (gp45) of equine infectious anemia virus.
abstract: Defined segments of the transmembrane envelope glycoprotein (gp45) of equine infectious anemia virus were expressed as TrpLE fusion proteins and examined for their reactivity in Western immunoblots against a diverse panel of equine immune sera. The most immunogenic region of gp45 was localized to its amino terminus, positioned between the hydrophobic fusion and the transmembrane domains. A series of overlapping synthetic peptides were used in enzyme-linked immunosorbent assays to define an immunodominant epitope within this region. In contrast, the carboxy-terminal half of gp45 displayed both weak and variable immunoreactivity with equine immune sera.
authors.affiliation: Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pennsylvania 15261.
authors.forename: Y H
authors.initials: YH
authors.lastname: Chong
authors.forename: J M
authors.initials: JM
authors.lastname: Ball
authors.forename: C J
authors.initials: CJ
authors.lastname: Issel
authors.forename: R C
authors.initials: RC
authors.lastname: Montelaro
authors.forename: K E
authors.initials: KE
authors.lastname: Rushlow
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D002462:Cell Membrane / Q000648:ultrastructure; D004279:DNA, Viral / Q000235:genetics; D004859:Equine Infectious Anemia / Q000276:immunology; D005814:Genes, Viral; D006736:Horses / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D004860:Infectious Anemia Virus, Equine / Q000235:genetics / Q000276:immunology*; D008961:Models, Structural; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000276:immunology; D015183:Restriction Mapping; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D004279:DNA, Viral; D007106:Immune Sera; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; C066797:transmembrane envelope glycoprotein gp45, retrovirus