Regeneration of CALLA (CD10+), TdT+ and double-positive cells in the bone marrow and blood after autologous bone marrow transplantation.
In this prospective study we investigated the frequency of CD10+, TdT+ and CD10+TdT+ mononuclear cells in the bone marrow (BM) and peripheral blood (PB) before and after autologous bone marrow transplantation (ABMT). 49 patients treated for acute lymphoblastic or myeloblastic leukaemia, malignant lymphoma or multiple myeloma were included. A significant increase in CD10+ cells occurred in BM in both children and adults after ABMT. In children, we also found a significant increase in CD10+ cells in PB. In individual patients remaining in remission, up to 34% CD10+ cells having a normal Ig kappa/lambda light chain ratio were recorded after ABMT. In children, the percentage of TdT+ and CD10+ TdT+ cells increased significantly in BM. In most cases the CD10/TdT-ratio was greater than 1.0, but during early regeneration after ABMT this ratio was less than 1.0 in several patients remaining in complete remission. In patients remaining in remission, CD10+TdT+ cells were detected in the blood in only 2 out of 140 samples tested, and the proportion of these cells never exceeded 0.03%. We conclude that quantitation of CD10+TdT+ cells in peripheral blood is helpful in the evaluation of complete remission in patients treated for pre-B-ALL.
Smedmyr
B
B
Department of Internal Medicine, University Hospital, Uppsala, Sweden.
Bengtsson
M
M
Jakobsson
A
A
Simonsson
B
B
Oberg
G
G
Tötterman
T H
TH
D000328:Adult; D000943:Antigens, Differentiation / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D014408:Biomarkers, Tumor; D001773:Blood Cells / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology*; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002648:Child; D004253:DNA Nucleotidylexotransferase / Q000096:biosynthesis; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis; D016130:Immunophenotyping; D007948:Leukemia, Monocytic, Acute / Q000276:immunology / Q000601:surgery; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear; D008223:Lymphoma / Q000276:immunology / Q000601:surgery; D009101:Multiple Myeloma / Q000276:immunology / Q000601:surgery; D015260:Neprilysin; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000601:surgery; D011446:Prospective Studies; D014182:Transplantation, Autologous
D000943:Antigens, Differentiation; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D007147:Immunoglobulin Light Chains; D004253:DNA Nucleotidylexotransferase; D015260:Neprilysin