Suppression by sorted CD8+CD11b- cells from T-cell growth factor-activated peripheral blood lymphocytes on cytolytic activity against tumour in patients with gastric carcinoma.
To confirm the phenotypic characteristics of lymphokine-activated suppressor (LAS)effector cells, we isolated CD8+CD11b- and CD8-CD11b- cells from T-cell growth factor (TCGF)-activated peripheral blood lymphocytes (PBL) in 7 patients with gastric carcinoma (4 non-resectable and 3 resectable carcinoma) and 3 healthy controls. Sorted CD8+CD11b- cells from 3 of the patients with non-resectable carcinoma and from 1 of the patients with resectable carcinoma showed LAS cell activity. However, the LAS cell activity could not be observed in CD8+CD11b- cells from healthy controls. In addition, a sorted CD8-CD11b- subset of cells from both cancer patients and control did not express any suppressive activity. These facts clearly show that the cell populations responsible for suppression of cell-mediated antitumour immunity reside within CD8+CD11b- T-cells, at least in patients with advanced carcinoma.
Ebihara
T
T
Department of Internal Medicine, University of Tsukuba, Japan.
Sakai
N
N
Koyama
S
S
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000276:immunology; D002469:Cell Separation; D003602:Cytotoxicity, Immunologic; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology*
D015703:Antigens, CD; D007376:Interleukin-2