Molecular characterization of G-protein coupled receptors: isolation and cloning of a D1 dopamine receptor.
This article summarizes the recent progress our laboratory has made in understanding the molecular characteristics of the D1 dopamine receptor. The D1 dopamine receptor from rat striatum has been purified to near homogeneity using a combination of several chromatographic steps. Furthermore, the gene for the human D1 dopamine receptor has been cloned, sequenced, and expressed. The cloned receptor has all the pharmacologic and biochemical properties of the classical D1 receptor coupled to adenylyl cyclase which has been previously described in the central nervous system.
Gingrich
J A
JA
Department of Cell Biology, Duke University Medical Center, Durham, N.C.
Dearry
A
A
Falardeau
P
P
Fremeau
R T
RT
Bates
M D
MD
Caron
M G
MG
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D019204:GTP-Binding Proteins / Q000378:metabolism*; D015870:Gene Expression; D008969:Molecular Sequence Data; D011954:Receptors, Dopamine / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D017447:Receptors, Dopamine D1; D011994:Recombinant Proteins
D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; D011994:Recombinant Proteins; D019204:GTP-Binding Proteins