Changes of ligand binding after membrane phosphorylation.
Phosphorylation of synaptic plasma brain membranes (SPM) causes a decrease of the specific binding of demethylated tricyclic antidepressants (TCA) and changes the affinity of 3H-imipramine and 3H-desmethylimipramine binding. The decrease of TCA binding was found also in lymphocyte membranes. In platelet membranes a decreased binding was found only with demethylated dibenzazepine derivatives. Bmax and Kd values are also decreased in the presence of phosphatidic acid or alpha-glycerolphosphate.
Krulík
R
R
Psychiatric Research Unit, Faculty of Medicine, Charles University, Praha, Czechoslovakia.
Bures
P
P
Simůnková
D
D
Havránek
M
M
D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000378:metabolism*; D002417:Cattle; D003640:Dealkylation; D003891:Desipramine / Q000378:metabolism; D003984:Dibenzazepines / Q000378:metabolism; D006801:Humans; D007099:Imipramine / Q000031:analogs & derivatives / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008024:Ligands; D010766:Phosphorylation; D013570:Synaptic Membranes / Q000378:metabolism*
D000929:Antidepressive Agents, Tricyclic; D003984:Dibenzazepines; D008024:Ligands; C036691:didesipramine; D007099:Imipramine; D003891:Desipramine