title: Increase in spermine content coordinated with siderophore production in Paracoccus denitrificans.
abstract: Spermine is present in relatively low amounts in Paracoccus denitrificans cultured aerobically in an ammonium succinate minimal salts medium supplemented with 50 microM iron(III). However, in iron-deprived cultures [minimal salts medium containing 0.5 microM iron(III)], spermine content increases by an order of magnitude in coordination with the well-known responses to iron derivation, e.g., derepression of siderophore synthesis and siderophore excretion. When iron-deprived cultures exhibiting both high spermine content and strong siderophore production are reseeded into fresh minimal salts medium containing 50 microM iron[III], both siderophore production and spermine content fall rapidly. Five hours after iron supplementation, spermine is below limits of detection. These results suggest a specific role for spermine in the response of P. denitrificans to low-iron stress.
authors.affiliation: Department of Medicinal Chemistry, J. Hillis Miller Health Science Center, University of Florida, Gainesville 32610-0485.
authors.forename: R J
authors.initials: RJ
authors.lastname: Bergeron
authors.forename: W R
authors.initials: WR
authors.lastname: Weimar
mesh_terms: D002851:Chromatography, High Pressure Liquid; D007501:Iron / Q000378:metabolism; D007502:Iron Chelating Agents / Q000737:chemistry / Q000378:metabolism*; D010231:Paracoccus denitrificans / Q000378:metabolism*; D017262:Siderophores; D013096:Spermine / Q000378:metabolism*
chemical_list: D007502:Iron Chelating Agents; D017262:Siderophores; D013096:Spermine; D007501:Iron