title: Peripheral blood and synovial fluid T cells differ in their response to alloantigens and recall antigens presented by dendritic cells.
abstract: Properties of T cells from inflammatory lesions were analysed by comparing the response of peripheral blood (PB) and synovial fluid (SF) T cells from 19 patients with a range of arthropathies to enriched allogeneic dendritic cells (DC) in a primary mixed leucocyte reaction (MLR). In 17 patients the proliferative response of SF T cells was significantly (P less than 0.05) less than that of PB lymphocytes. The reduced response of SF T cells was observed in all disease categories studied and could not be attributed to differences in cell number requirements or response kinetics. Addition of recombinant interleukin-2 enhanced the response of SF T cells in a dose-dependent manner. Cell mixing experiments suggested that active suppression was not the underlying mechanism of the poor MLR response of SF T cells. In contrast to the MLR response. SF T cells were able to mount vigorous proliferative responses to recall antigen presented by autologous antigen-presenting cells. The possibility is discussed that T cells compartmentalized at inflammatory lesions are a unique population with a diminished ability to interact with DC and respond to primary stimuli but an ability to respond to secondary antigenic challenge.
authors.affiliation: Antigen Presentation Research Group, Clinical Research Centre, Harrow, Middlesex, England.
authors.forename: A J
authors.initials: AJ
authors.lastname: Stagg
authors.forename: B
authors.initials: B
authors.lastname: Harding
authors.forename: R A
authors.initials: RA
authors.lastname: Hughes
authors.forename: A
authors.initials: A
authors.lastname: Keat
authors.forename: S C
authors.initials: SC
authors.lastname: Knight
mesh_terms: D001168:Arthritis / Q000276:immunology* / Q000473:pathology; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007519:Isoantigens / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D011994:Recombinant Proteins; D013582:Synovial Fluid / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D007376:Interleukin-2; D007519:Isoantigens; D011994:Recombinant Proteins