title: Inhibition of SV40 gene expression by microinjected small antisense RNA and DNA molecules.
abstract: We tested the impact of antisense RNA and DNA molecules on SV40 gene expression by microinjection into TC7 cells. Short antisense stretches, complementary to either hairpin or loop structures on the T antigen mRNA, inhibited T antigen synthesis. In contrast, full-length antisense RNA and DNA molecules did not effect T antigen synthesis.
authors.affiliation: Institut für Molekularbiologie und Biochemie der Freien Universität Berlin, FRG.
authors.forename: M
authors.initials: M
authors.lastname: Graessmann
authors.forename: G
authors.initials: G
authors.lastname: Michaels
authors.forename: B
authors.initials: B
authors.lastname: Berg
authors.forename: A
authors.initials: A
authors.lastname: Graessmann
mesh_terms: D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis*; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D003198:Computer Simulation; D016373:DNA, Antisense / Q000494:pharmacology*; D004279:DNA, Viral / Q000187:drug effects / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D005814:Genes, Viral; D007700:Kinetics; D008845:Microinjections; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016372:RNA, Antisense / Q000494:pharmacology*; D012367:RNA, Viral / Q000187:drug effects / Q000494:pharmacology; D051381:Rats; D013539:Simian virus 40 / Q000187:drug effects / Q000235:genetics*
chemical_list: D000952:Antigens, Polyomavirus Transforming; D016373:DNA, Antisense; D004279:DNA, Viral; D016372:RNA, Antisense; D012367:RNA, Viral