title: PCR-SSO typing for DR4-Dw subtypes: application to unrelated bone marrow transplant donor selection.
abstract: Thirty-seven DR4-positive patient-unrelated bone marrow donor pairs previously DR/DQ restriction fragment length polymorphism (RFLP) typed and tested in mixed lymphocyte culture (MLC), have been DR4-Dw subtyped retrospectively using sequence specific oligonucleotide probes. We found that DR4-Dw subtyping substantially increased the accuracy of pre-MLC matching and could potentially accelerate donor searches by avoiding unnecessary MLC tests on Dw-mismatched donors.
authors.affiliation: United Kingdom Transplant Service, Bristol.
authors.forename: T M
authors.initials: TM
authors.lastname: Clay
authors.forename: M R
authors.initials: MR
authors.lastname: Howard
authors.forename: J L
authors.initials: JL
authors.lastname: Bidwell
authors.forename: E A
authors.initials: EA
authors.lastname: Bidwell
authors.forename: P A
authors.initials: PA
authors.lastname: Raymond
authors.forename: J E
authors.initials: JE
authors.lastname: Evans
authors.forename: B A
authors.initials: BA
authors.lastname: Bradley
mesh_terms: D016026:Bone Marrow Transplantation / Q000276:immunology*; D015342:DNA Probes; D015804:HLA-DR4 Antigen / Q000145:classification / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D007959:Lymphocyte Culture Test, Mixed; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D014019:Tissue Donors
chemical_list: D015342:DNA Probes; D015804:HLA-DR4 Antigen; D009838:Oligodeoxyribonucleotides