Curved DNA without AA/TT dinucleotide step.
The evidence is accumulating that dinucleotide steps other than AA/TT affect DNA flexure of AnTm (m + n greater than = 4) containing fragments. However, it is not clear whether macroscopic DNA flexure without AA/TT steps might occur. In this paper we demonstrate the anomaly in electrophoretic mobility of non AA/TT repetitive DNA sequences which is a function of sequence phasing. Therefore, our results show that PyPu (TA) and AG/CT steps, angulary separated by close to 180 degrees from Pu/Py (GC) and GG/CC steps, bend DNA, even in the absence of AnTm tracts.
Brukner
I
I
Institute of Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia.
Jurukovski
V
V
Konstantinović
M
M
Savić
A
A
D000227:Adenine Nucleotides / Q000737:chemistry; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D012091:Repetitive Sequences, Nucleic Acid; D013942:Thymine Nucleotides / Q000737:chemistry
D000227:Adenine Nucleotides; D009711:Nucleotides; D009838:Oligodeoxyribonucleotides; D013942:Thymine Nucleotides; D004247:DNA