title: Primary structure of the alpha-subunit of vacuolar-type Na(+)-ATPase in Enterococcus hirae. Amplification of a 1000-bp fragment by polymerase chain reaction.
abstract: A 1000-bp fragment of Enterococcus hirae genomic DNA was amplified by the polymerase chain reaction method, using the oligonucleotide primers designed from amino acid sequences of both amino-terminal and a tryptic fragment of the Na(+)-ATPase alpha-subunit in this organism. DNA sequencing of this product revealed that the amino acid sequence of Na(+)-ATPase alpha-subunit is highly homologous to the corresponding sequences of large (alpha) subunits of vacuolar (archaebacterial) type H(+)-ATPases, supporting our proposal [Kakinuma, Y. and Igarashi, K. (1990) FEBS Lett. 271, 97-101] that the Na(+)-ATPase of this organism belongs to the vacuolar-type ATPase.
authors.affiliation: Faculty of Pharmaceutical Sciences, Chiba University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kakinuma
authors.forename: K
authors.initials: K
authors.lastname: Igarashi
authors.forename: K
authors.initials: K
authors.lastname: Konishi
authors.forename: I
authors.initials: I
authors.lastname: Yamato
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D027682:Cation Transport Proteins; D004269:DNA, Bacterial / Q000235:genetics; D016983:Enterococcus / Q000201:enzymology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D014617:Vacuoles / Q000201:enzymology*
chemical_list: D027682:Cation Transport Proteins; D004269:DNA, Bacterial; D000251:Adenosine Triphosphatases; C021838:sodium-translocating ATPase