Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis A antigen.
Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HAV HRP conjugate; in the second (COMP-2), test sera and anti-HAV HRP conjugate competed for HAV epitopes previously adsorbed to anti-HAV IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes.
Vitral
C L
CL
Departamento of Virology, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Gaspar
A M
AM
Yoshida
C F
CF
D000956:Antigens, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006506:Hepatitis A / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards*; D007074:Immunoglobulin G / Q000032:analysis*; D012015:Reference Standards
D000956:Antigens, Viral; D007074:Immunoglobulin G