title: Plant biochemistry of xenobiotics: isolation and properties of soybean O- and N-glucosyl and O- and N-malonyltransferases for chlorinated phenols and anilines.
abstract: O-Glucosyltransferase (O-GT), O-malonyltransferase (O-MAT), N-glucosyltransferase (N-GT), and N-malonyltransferase (N-MAT) activities have been detected in cultured soybean cells, using pentachlorophenol and 3,4-dichloroaniline as xenobiotic standard substrates. The O-GT was purified approximately 1000-fold, and the N-MAT approximately 70-fold. There was an extensive copurification of O-GT and O-MAT. The following functional molecular weight values were obtained, 47 kDA (O-GT), 48 kDA (O-MAT) 43 kDa (N-GT), and 48 kDa (N-MAT). O-GT and N-MAT appeared to be monomeric polypeptides with isoelectric points of approximately 4.8 and approximately 6.1, respectively. The O-GT, N-GT, and N-MAT activities had marked substrate specificities for chlorinated aromatic xenobiotics and thus illustrate the existence of plant isoenzymes with specificity for xenobiotics.
authors.affiliation: GSF-Institut für Biochemische Pflanzenpathologie, Neuherberg, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Sandermann
authors.forename: R
authors.initials: R
authors.lastname: Schmitt
authors.forename: H
authors.initials: H
authors.lastname: Eckey
authors.forename: T
authors.initials: T
authors.lastname: Bauknecht
mesh_terms: D000217:Acyltransferases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000814:Aniline Compounds / Q000378:metabolism*; D002384:Catalysis; D005964:Glucosyltransferases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D010416:Pentachlorophenol / Q000378:metabolism*; D013025:Glycine max / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D000814:Aniline Compounds; D007527:Isoenzymes; C014464:3,4-dichloroaniline; D010416:Pentachlorophenol; D000217:Acyltransferases; D005964:Glucosyltransferases