Modification of mitogenic response to concanavalin A of lymphocytes from old-aged donors by ion channel blockers.
Mitogenic response of lymphocytes from young and old donors to Concanavalin A in the absence or presence of Na+ channel blocker--saxitoxin--was compared. Effect of Ca++ channel blockers (Verapamil Diltiazem) on mitogenic response of lymphocytes from old subjects was also examined. It was found that the mitogenic response to Concanavalin A of lymphocytes from donors was significantly lower than that of young ones. In the presence of Saxitoxin the enhancement of the response was noticeable in both groups and the mean responses were similar. However, among the old donors, opposite to the young ones, very high and very poor responders were present. Verapamil and Diltiazem caused significant reduction of mitogenic response to Concanavalin A in the group of old donors. These observations suggest a possible effect of ion channel blockers on immunological functions which would have special significance for old organisms.
Myśliwski
A
A
Department of Histology, Medical Academy, Gdańsk, Poland.
Witkowski
J M
JM
Myśliwska
J
J
Bigda
J
J
D000328:Adult; D000367:Age Factors; D000368:Aged; D002121:Calcium Channel Blockers / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D012530:Saxitoxin / Q000494:pharmacology
D002121:Calcium Channel Blockers; D003208:Concanavalin A; D012530:Saxitoxin