The CD45RA molecule is expressed in naive murine CTL precursors but absent in memory and effector CTL.
We have studied the expression of the CD45RA molecule in murine cytotoxic T lymphocytes (CTL) specific for the allogeneic H-2Kb molecule at different stages of differentiation. The CD45RA phenotype of naive H-2Kb-specific CTL precursors has been determined using primary in vitro CTL responses. For the analysis of memory CTL we have immunized mice in vivo followed by restimulation in vitro. We have also determined the CD45RA expression at the CTL effector stage. Our results show that among naive CD8+ T cells both the CD45RA+ and the CD45RA- subpopulations can mount Kb-specific CTL responses. In contrast, memory CTL responses are mediated only by the CD8+ CD45RA+ T cell subpopulation. Similarly, effector CTL are CD8+ CD45RA- while the CD8+ CD45RA+ subpopulation does not exhibit specific cytolytic activity. The data indicate that CD45RA expression changes during CTL differentiation and that memory as well as effector CTL lack this marker.
Marvel
J
J
Imperial Cancer Research Fund, University College London, UK.
Lightstone
E
E
Samberg
N L
NL
Ettinghausen
D
D
Stauss
H J
HJ
D000818:Animals; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D002454:Cell Differentiation; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006649:Histocompatibility Antigens; D007156:Immunologic Memory; D017493:Leukocyte Common Antigens; D051379:Mice; D008809:Mice, Inbred C3H; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D006649:Histocompatibility Antigens; D017493:Leukocyte Common Antigens