Induction of chromosomal aberrations by restriction endonucleases encapsulated in liposomes.
We used liposomes to deliver the restriction endonucleases BamHI and SmaI into human heteroploid HEp-2 cells. With this method very low concentrations of enzymes (2 units/ml) were active in the production of chromosomal aberrations. SmaI, which produces blunt-ended double-strand breaks in the DNA molecule, induces chromosomal aberrations more effectively than BamHI, which produces cohesive ends. Our results indicate that liposomes are suitable vectors for introducing restriction endonucleases into cultured human cells.
Mixich
F
F
Department of Cell Biology, Faculty of Medicine Craiova, Romania.
D002478:Cells, Cultured; D004249:DNA Damage; D004262:DNA Restriction Enzymes / Q000008:administration & dosage / Q000494:pharmacology*; D015257:DNA-Cytosine Methylases / Q000008:administration & dosage / Q000494:pharmacology; D015252:Deoxyribonucleases, Type II Site-Specific / Q000008:administration & dosage / Q000494:pharmacology; D004337:Drug Carriers; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes; D016296:Mutagenesis
D004337:Drug Carriers; D008081:Liposomes; C042525:DNA modification methylase BamHI; D015257:DNA-Cytosine Methylases; D004262:DNA Restriction Enzymes; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific