[Application and preparation of a BUN Test Strip].
This paper introduces a dry chemical method for BUN determination and a procedure for the preparation of the BUN Test Strip. Urease, indicator, buffer, nonreactive stabilizers and fillers are immobilized on a piece of special paper, and then a thin film is laid on top to protect against interference from endogenous blood materials. After the paper is dried, it is cut into small strips and ready to use. When the sample is loaded on the strip, the enzymatic reaction can be carried out in one minute, and with a color plate, one can obtain semiquantitative BUN concentration. One-day and day-to-day precision variations (CV%) were 2.6%-5.0% and 5.9%-7.1%, respectively. There was a good correlation between our BUN Strip and the Ames BUN Strip: r = 0.98, y = 0.96x -2.4 (x = Ames BUN Strip).
Li
X
X
PUMC Hospital, Beijing.
D001806:Blood Urea Nitrogen; D011934:Reagent Strips; D014510:Urease
D011934:Reagent Strips; D014510:Urease