In-vitro DNA synthesis of keratinocytes in normal human skin, psoriasis, seborrhoeic keratosis, Bowen's disease and basal cell carcinoma.
The DNA-synthesizing cells in benign and malignant keratotic diseases including psoriasis, seborrhoeic keratosis, Bowen's disease and basal cell carcinoma were studied. Cells in S-phase were labelled after small pieces of the lesions were incubated in tissue culture medium that contained 5-bromo-2'-deoxyuridine (BrdU). The labelling of DNA was analysed using the labelling index and pattern. Although the malignant diseases tended to show a higher DNA labelling index, neither this nor the loss of polarity alone was associated with malignancy. However, a higher DNA labelling index together with a loss of polarity was characteristic for malignancy.
Morimoto
Y
Y
Department of Dermatology, Sapporo Medical College, Japan.
Saga
K
K
Bando
M
M
Takahashi
M
M
D001913:Bowen's Disease / Q000378:metabolism; D001973:Bromodeoxyuridine; D002280:Basal Cell Carcinoma / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D012628:Dermatitis, Seborrheic / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D015603:Keratinocytes / Q000378:metabolism*; D007642:Keratosis / Q000378:metabolism; D011565:Psoriasis / Q000378:metabolism; D012867:Skin / Q000378:metabolism*; D012871:Skin Diseases / Q000378:metabolism*; D012878:Skin Neoplasms / Q000378:metabolism
D004247:DNA; D001973:Bromodeoxyuridine