Expression of HSV-1 and HSV-2 glycoprotein G in insect cells by using a novel baculovirus expression vector.
Herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) glycoprotein G (gG-1 and gG-2) were expressed in insect cells from recombinant baculoviruses (AcDSMgG-1 and AcDSMgG-2, respectively) constructed using a novel baculovirus transfer vector, pAcDSM. This vector allows the coding region of a foreign gene to be precisely linked to the baculovirus polyhedrin gene at the translation initiation site and retains the native polyhedrin translation initiation environment. Fourfold more gG-1, with a higher ratio of glycosylated to unglycosylated product, was produced by AcDSMgG-1 than by Ac373'gG-1, a recombinant baculovirus which differs from AcDSMgG-1 by the presence of 21 extraneous nucleotides in the 5' nontranslated sequence. gG-1 and gG-2 expressed in recombinant baculovirus-infected insect cells undergo cotranslational N-linked glycosylation, but the overall processing of the proteins differs from that observed in HSV-1 or HSV-2-infected cells. Despite these differences, baculovirus-expressed gG-1 and gG-2 were recognized in a HSV type-specific manner by human serum specimens.
Sánchez-Martínez
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Molecular Biology Department, Biokit S.A., Lliçà d'Amunt, Barcelona, Spain.
Pellett
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D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D005822:Genetic Vectors; D007313:Insecta; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000276:immunology; D018139:Simplexvirus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology
D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C045464:glycoprotein G, herpes simplex virus type 2; C050591:glycoprotein gG-1, herpes simplex virus type 1