title: Lymphokine activated killer cells and interleukin-2 production in patients with cervical carcinoma.
abstract: Lymphokine activated killer cells were generated from peripheral blood lymphocytes (PBL) in patients with cervical carcinoma. The cytotoxic potential of these cells was determined against three tumor cell lines (K-562, RAJI and MCF-7) using a short-term 51Cr-release assay. Both normals and patients showed comparable levels of LAK activity. Phenotypic analysis of cells having LAK activity showed a heterogeneous population. A significant increase in the expression of CD25 marker (IL-2R, Tac) of PBL population was observed after IL-2 culture. In addition, the indigenous ability of peripheral blood mononuclear cells to produce IL-2 in response to mitogen PHA was almost the same as observed in case of normal individuals.
authors.affiliation: Immunology Division, Tata Memorial Centre, Bombay, India.
authors.forename: A K
authors.initials: AK
authors.lastname: De
authors.forename: K G
authors.initials: KG
authors.lastname: Jhaver
authors.forename: M R
authors.initials: MR
authors.lastname: Kamat
authors.forename: J J
authors.initials: JJ
authors.lastname: Nadkarni
mesh_terms: D000328:Adult; D000368:Aged; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis; D017252:CD3 Complex; D015704:CD4 Antigens / Q000096:biosynthesis; D016827:CD8 Antigens / Q000096:biosynthesis; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000096:biosynthesis*; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology* / Q000378:metabolism; D008875:Middle Aged; D010835:Phytohemagglutinins; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D007376:Interleukin-2; D010835:Phytohemagglutinins; D011948:Receptors, Antigen, T-Cell