Effect of cyclosporin A on B cell maturation and differentiation.
The activation and differentiation of resting B cells into Ig secreting cells are regulated by T cells, macrophages and their secreted factors. The present study evaluated the effect of cyclosporin A (CsA) on this process. Peripheral blood lymphomonocytes (PBMC) drawn from healthy donors were stimulated with protein A (PA) or with lipopolysaccharides plus pokeweed (LPS+PWM) in either the presence or the absence of CsA. Phenotypic B cell changes and immunoglobulin production was then analyzed. The data revealed that CsA decreased the expression of B cell surface receptors of the activation phase, and enhanced the resting phase receptors. Different effects of CsA were found on B cell differentiation, depending on its induction by PA or LPS+PWM. In the first system, CsA decreased the expression of differentiation phase receptors and the secretion of free Ig. In cultures stimulated with LPS+PWM, CsA increased the differentiated phase receptors and Ig secretion. Thus, CsA seemed to act as a blocking agent of the activation phase and as a modulator of the differentiation phase and of IgG secretion, depending upon the antigen used for stimulation.
Lun
M T
MT
Second Department of Microbiology, Faculty of Medicine and Surgery, University La Sapienza, Italy.
Cochi
S
S
Fioravanti
D
D
Nazzari
C
C
Raponi
G
G
Mannella
E
E
Di Tommaso
G
G
Mancini
C
C
Filadoro
F
F
D000954:Antigens, Surface / Q000187:drug effects; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D002454:Cell Differentiation / Q000187:drug effects; D016572:Cyclosporine / Q000494:pharmacology*; D006801:Humans; D007074:Immunoglobulin G / Q000187:drug effects; D066298:In Vitro Techniques; D008070:Lipopolysaccharides; D008213:Lymphocyte Activation / Q000187:drug effects; D011043:Pokeweed Mitogens; D013205:Staphylococcal Protein A
D000954:Antigens, Surface; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D011043:Pokeweed Mitogens; D013205:Staphylococcal Protein A; D016572:Cyclosporine