Ascorbic acid prevents the inhibition of DNA synthesis induced by high glucose concentration in cultured human endothelial cells.
Effects of glucose and ascorbic acid on in vitro collagen and DNA synthesis were evaluated in human umbilical vein endothelial cells. Ascorbic acid significantly potentiated collagen synthesis. Incubation of cells with elevated glucose concentration for 24h significantly reduced [3H]-thymidine uptake (2h). However, the addition of ascorbic acid (0.1 mM) dramatically prevented the inhibition of thymidine uptake. Based on these data, it is suggested that ascorbic acid supplementation in diabetics may prevent or ameliorate diabetic angiopathy.
Okuda
Y
Y
Department of Internal Medicine, University of Tsukuba, Ibaraki, Japan.
Nagahama
M
M
Mizutani
M
M
Bannai
C
C
Yamashita
K
K
D001205:Ascorbic Acid / Q000494:pharmacology*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D013936:Thymidine / Q000378:metabolism; D014471:Umbilical Veins
D003094:Collagen; D004247:DNA; D005947:Glucose; D001205:Ascorbic Acid; D013936:Thymidine