The effect of organic chlorine compounds and their metabolites present in human milk on newborn mice.
The effect of pesticides and their metabolites (DDE, DDT, DDD, alpha, beta and gamma-HCH and PCBs) isolated from human milk on the blood and liver morphology of the mouse were studied. Mouse neonates were fed an extract of the organochlorine compounds in linseed oil for a period of 6 weeks. The lowest dose used in the experiment equalled that which a human infant can receive with its mother's milk, calculated per gram of body weight. Doses 10 and 100 times higher were also used. At the end of the experiment, tissue samples for electron microscopy and blood samples for haemotological examination were taken. Haematological examinations, in mice receiving pesticides, revealed a significant rise in the number of Heinz bodies in erythrocytes and an increased number of lymphocytes. These changes were related to the concentrations of the organic chlorine compounds that the animals were given. Electronmicroscopy demonstrated that organochlorine pesticides at doses equal to that might be received by infants caused proliferation of the smooth endoplasmic reticulum in hepatocytes without any clear damage to other subcellular structures. Doses 10 and 100 times higher resulted in more extensive proliferation of the smooth endoplasmic reticulum, reduction of the rough elements of the endoplasmic reticulum and damage to mitochondria. The latest changes were associated with an increased number of prominent Kupffer cells and the appearance of immigratory cells with traits characteristic of lymphocytes and monocytes.
Lembowicz
K
K
Chair and Clinical Department of Internal Diseases, Faculty of Veterinary Medicine, Agricultural Academy, Warsaw, Grochowska, Poland.
Sitarska
E
E
Górski
T
T
Ludwicki
J K
JK
D000284:Administration, Oral; D000818:Animals; D000831:Animals, Newborn; D001772:Blood Cell Count / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D006400:Hematocrit; D006454:Hemoglobins / Q000187:drug effects; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008895:Milk, Human / Q000737:chemistry*
D006454:Hemoglobins; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides