IL-4-producing murine T helper cell line provides help for in vitro production of IgE.
Studies of the regulation of the IgE synthesis have been hampered by the difficulties in making lymphocytes produce IgE in vitro. Synthesis of IgE, IgG1 and IgM were investigated in a murine coculture system in which a conalbumin-specific T helper cell line of Th2 type was cultured together with nonadherent splenocytes from normal MHC-matched mice. Help provided by the antigen-stimulated T cell line induced significant IgE production (20 ng/ml), along with IgG1 (5 micrograms/ml) and IgM (250 micrograms/ml). Immunoglobulin synthesis in cultures was detectable at day 3-4 and culminated at day 7-8. IL-4 production was observed within the first 2 days of culture. The kinetics of the synthesis of the three isotypes were parallel. Stimulation of the Th2 cell line with Con A produced dose-response curves resembling those after antigen stimulation, whereas supernatant from these cells was unable to induce synthesis of IgE. The presence of interferon-gamma in the cultures, inhibited synthesis of IgE, IgM and IgG1, but this inhibition was not due to interference with the IL-4 production, which increased in the presence of high doses of interferon-gamma.
Poulsen
L K
LK
Laboratory of Medical Allergology, National University Hospital, Copenhagen, Denmark.
Katamura
K
K
Ishizaka
K
K
D000818:Animals; D002478:Cells, Cultured; D003207:Conalbumin / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000502:physiology; D015847:Interleukin-4 / Q000502:physiology*; D051379:Mice; D008806:Mice, Inbred AKR; D008809:Mice, Inbred C3H; D011863:Radioimmunoassay; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D003208:Concanavalin A; D003207:Conalbumin; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma