Polymerase chain reaction amplification of the genome of infectious bronchitis virus.
The polymerase chain reaction (PCR) was used to amplify a portion of the genome of infectious bronchitis virus (IBV). Two synthetic primers that annealed to segments of the IBV genome in the matrix and nucleocapsid genes facilitated PCR amplification of a 1020-base sequence. Amplification was successful using template DNA from an IBV sequence-containing plasmid and using copy DNA created by reverse transcription of IBV genomic RNA. The PCR product was the expected size and had the expected nucleotide sequence.
Andreasen
J R
JR
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30605.
Jackwood
M W
MW
Hilt
D A
DA
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D004279:DNA, Viral / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D005784:Gene Amplification; D001351:Infectious bronchitis virus / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000737:chemistry*; D013698:Templates, Genetic; D014158:Transcription, Genetic
D004279:DNA, Viral; D012367:RNA, Viral