Expression of HLA antigens and T cell infiltrates in chronic viral hepatitis. A comparison of biopsy and fine-needle aspiration findings.
To evaluate the expression of hepatocyte HLA Class I and II antigens and the infiltration of CD8-positive lymphocytes in patients with chronic hepatitis, cells obtained by the technique of fine-needle aspiration were compared with those obtained by percutaneous liver biopsy. In general, correlation between cytological and histological specimens of HLA Class I and Class II antigen expression and CD8 lymphocyte infiltration was excellent when comparing results of the core biopsy expressed semiquantitatively, and results from fine-needle aspiration quantitatively as percentage of cells stained. Although the major potential of multiple fine-needle aspiration sampling probably will be in the surveillance of patients who have received orthotopic liver transplants, the results of this study indicate that fine-needle aspiration can provide a useful technique for following antigen expression and inflammatory changes in studies in which frequent periodic samples may be necessary to monitor efficacy or toxicity of treatment regimens.
Krawitt
E L
EL
Hopital E. Herriot, INSERM U80, Lyon, France.
Zannier
A
A
Chossegros
P
P
Gerard
F
F
Chevallier
M
M
Mutin
M
M
Trepo
C
C
Touraine
J L
JL
D001706:Biopsy; D001707:Biopsy, Needle; D002908:Chronic Disease; D006680:HLA Antigens / Q000032:analysis*; D006681:HLA-D Antigens / Q000032:analysis*; D006525:Hepatitis, Viral, Human / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D006801:Humans; D007958:Leukocyte Count; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D006680:HLA Antigens; D006681:HLA-D Antigens; D015395:Histocompatibility Antigens Class I