Complement in vitro inhibition by a low-sulfate chondroitin sulfate (matrix).
In this study, the ability of low-sulfate chondroitin sulfate (Matrix), a glycosaminoglycan that does not exhibit anticoagulant action, to inhibit the activation of complement has been evaluated. Two different in vitro tests were employed: lysis of mouse splenocytes sensitized with monoclonal antibodies and lysis of red blood cells sensitized using a rabbit antiserum towards those cells. In both tests, lyophilized rabbit complement was used as complement source. The low-sulfate chondroitin sulfate Matrix has been compared with a hypersulfated one (Arteparon). Both compounds share anticomplementary activity in vitro although potency appears to be related to the sulfate content of the chondroitin.
Biffoni
M
M
Institute of Pharmacology, University of Rome, La Sapienza, Italy.
Paroli
E
E
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D000911:Antibodies, Monoclonal; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D003167:Complement Activation / Q000187:drug effects; D015941:Complement Hemolytic Activity Assay; D003169:Complement Inactivator Proteins / Q000494:pharmacology*; D006025:Glycosaminoglycans / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000166:cytology; D051379:Mice; D011817:Rabbits
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000911:Antibodies, Monoclonal; D003169:Complement Inactivator Proteins; D006025:Glycosaminoglycans; C010034:Arteparon; D002809:Chondroitin Sulfates