Association of DNA helicase and primase activities with a subassembly of the herpes simplex virus 1 helicase-primase composed of the UL5 and UL52 gene products.
Herpes simplex virus 1 encodes a helicase-primase that is composed of the products of the UL5, UL8, and UL52 genes. A stable subassembly consisting of only the UL5 and UL52 gene products has been purified to near homogeneity from insect cells doubly infected with baculovirus recombinant for these two genes. The purified subassembly has the DNA-dependent ATPase, DNA-dependent GTPase, DNA helicase, and DNA primase activities that are characteristic of the three-subunit holoenzyme. The purified UL8 gene product, although required for viral DNA replication, neither exhibits these enzymatic activities nor stably associates with either the UL5 or the UL52 gene product.
Dodson
M S
MS
Department of Biochemistry, Beckman Center, Stanford University School of Medicine, CA 94305.
Lehman
I R
IR
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D002460:Cell Line; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003600:Cytosol / Q000201:enzymology; D004265:DNA Helicases; D019915:DNA Primase; D020558:GTP Phosphohydrolases / Q000235:genetics* / Q000302:isolation & purification; D005814:Genes, Viral; D046911:Macromolecular Substances; D009097:Multienzyme Complexes / Q000235:genetics / Q000302:isolation & purification; D012316:RNA Nucleotidyltransferases / Q000235:genetics* / Q000302:isolation & purification; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics*
D046911:Macromolecular Substances; D009097:Multienzyme Complexes; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D000251:Adenosine Triphosphatases; D020558:GTP Phosphohydrolases; D004265:DNA Helicases