Nucleotide sequence of cDNA coding for dianthin 30, a ribosome inactivating protein from Dianthus caryophyllus.
Rabbit antibodies raised against dianthin 30, a ribosome inactivating protein from carnation (Dianthus caryophyllus) leaves, were used to identify a full length dianthin precursor cDNA clone from a lambda gt11 expression library. N-terminal amino acid sequencing of purified dianthin 30 and dianthin 32 confirmed that the clone encoded dianthin 30. The cDNA was 1153 basepairs in length and encoded a precursor protein of 293 amino acid residues. The first 23 N-terminal amino acids of the precursor represented the signal sequence. The protein contained a carboxy-terminal region which, by analogy with barley lectin, may contain a vacuolar targeting signal.
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Department of Biological Sciences, University of Warwick, Coventry, U.K.
Bellosta
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Gromo
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Modena
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D
Keen
J N
JN
Roberts
L M
LM
Lord
J M
JM
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D000922:Immunotoxins; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases; D037121:Plant Lectins; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics*; D015183:Restriction Mapping; D054789:Ribosome Inactivating Proteins, Type 1; D012276:Ricin / Q000737:chemistry; D000076985:Saporins; D016415:Sequence Alignment; D015978:Trichosanthin / Q000737:chemistry
D000922:Immunotoxins; D037121:Plant Lectins; D010940:Plant Proteins; D021382:Protein Sorting Signals; D054789:Ribosome Inactivating Proteins, Type 1; D015978:Trichosanthin; D012276:Ricin; D009699:N-Glycosyl Hydrolases; D000076985:Saporins