Cytofluorometric determination of nuclear DNA content in gallbladder epithelium of patients with biliary stones.
Cytofluorometric determination of nuclear DNA content in fresh gallbladder epithelium of 11 patients with biliary stones were performed to assess the histological correlations and to evaluate the ploidy pattern affected by inflammation or regenerative proliferating benign epithelium associated with non-neoplastic or neoplastic diseases. 27% of the patients were classified to have pure cholesterol stones, 54% were classified to have mixed stones and 19% were classified to have black stones. All of the specimens show suggestion of chronic cholecystitis without any metaplastic or neoplastic change. The bile culture shows free of bacteria in all of the cases. Cytofluorometric nuclear DNA ploidy analysis shows 64% diploidy and 36% low ploidy patterns of histograms. It can be concluded that the low ploidy pattern which could lead to false positive diagnosis of aneuploidy and may be associated with regenerative proliferating benign epithelium with severe inflammatory changes.
Matin
M A
MA
1st Department of Surgery, School of Medicine, University of Tokushima, Japan.
Kunitomo
K
K
Komi
N
N
D000328:Adult; D000368:Aged; D002467:Cell Nucleus / Q000737:chemistry; D002769:Cholelithiasis / Q000737:chemistry* / Q000235:genetics; D004247:DNA / Q000032:analysis* / Q000235:genetics; D004848:Epithelium / Q000737:chemistry; D005260:Female; D005470:Fluorometry; D005704:Gallbladder / Q000737:chemistry*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011003:Ploidies
D004247:DNA