Structural analysis of a mature hst-1 protein with transforming growth factor activity.
A recombinant hst-1 protein produced in silkworm cells by a recombinant baculovirus, previously shown to be a potent mitogen for NIH3T3 cells and human endothelial cells, also stimulated anchorage-independent growth of NRK-49F cells. Amino acid sequence analysis revealed that the amino-terminal sequence with 58 amino acids was cleaved off in silkworm cells. These results indicated that the mature hst-1 protein consisting of 148 amino acids had transforming growth factor activity.
Miyagawa
K
K
Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.
Kimura
S
S
Yoshida
T
T
Sakamoto
H
H
Takaku
F
F
Sugimura
T
T
Terada
M
M
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000378:metabolism; D012831:Bombyx / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004727:Endothelium / Q000187:drug effects; D051519:Fibroblast Growth Factor 4; D005346:Fibroblast Growth Factors / Q000737:chemistry* / Q000494:pharmacology; D006133:Growth Substances / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000737:chemistry* / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000737:chemistry* / Q000494:pharmacology; D015291:Transforming Growth Factors / Q000737:chemistry / Q000378:metabolism*
C496445:FGF4 protein, human; D051519:Fibroblast Growth Factor 4; D006133:Growth Substances; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D005346:Fibroblast Growth Factors; D015291:Transforming Growth Factors