Specific radioimmunoassay to investigate rod outer segment phagocytosis by retinal pigment epithelium in vitro.
A sensitive radioimmunoassay has been developed which allows rapid quantitation of rod outer segment (ROS) phagocytosis by retinal pigment epithelial (RPE) explants in vitro. It involves the use of an antiopsin antiserum, in conjunction with 125I-protein A as a second antibody, and utilizes permeabilization with ethanol to distinguish between the binding and ingestion phases of phagocytosis. This procedure will be used in the future to investigate potential regulatory factors of ROS phagocytosis by retinal pigment epithelium and to evaluate animal models of retinal degeneration.
Gregory
C Y
CY
Department of Pharmacy, University of Strathclyde, Glasgow, UK.
Converse
C A
CA
Foulds
W S
WS
D000818:Animals; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005136:Eye Proteins / Q000276:immunology; D010587:Phagocytosis / Q000276:immunology*; D010786:Photoreceptor Cells / Q000276:immunology; D010857:Pigment Epithelium of Eye / Q000276:immunology*; D011863:Radioimmunoassay / Q000379:methods*; D015203:Reproducibility of Results; D012168:Retinal Pigments / Q000276:immunology; D012374:Rod Cell Outer Segment / Q000276:immunology*; D017299:Rod Opsins; D012680:Sensitivity and Specificity
D005136:Eye Proteins; D012168:Retinal Pigments; D017299:Rod Opsins