Time dependent release of tissue-type plasminogen activator and plasminogen activator inhibitor into the circulation of pigs during shock.
To investigate short-term activation and inhibition of fibrinolysis during shock, we studied plasma levels of tissue-type plasminogen activator (t-PA) and t-PA inhibition capacity (PAI) in anaesthetized pigs. t-PA in euglobulin fractions of plasma was measured by the conversion of plasminogen to plasmin in the presence of fibrin split products. Plasmin thus generated was measured in a chromogenic substrate assay. PAI was measured as plasma inhibition capacity for human melanoma t-PA. Controls (n = 8) had constant t-PA and PAI for 6 h. Lipopolysaccharide from Salmonella abortus equi in four different doses (n = 9 - 11), or live Escherichia coli (n = 3) induced a transient t-PA increase with peak values at 2 h. PAI decreased to 50% at 2 h and increased to 250% at 6 h. Phorbol myristate acetate (n = 7) induced no change of t-PA or PAI. Dextran sulphate (n = 4) produced a t-PA rise at 30 min, followed by a rapid decline. Endotoxin was an appropriate stimulus for activation and inhibition of fibrinolysis whereas phorbol ester failed to elicit this response.
Siebeck
M
M
Department of Surgery, Klinikum Innenstadt, University of Munich, Germany.
Spannagl
M
M
Hoffmann
H
H
Schramm
W
W
Fritz
H
H
D000818:Animals; D002863:Chromogenic Compounds / Q000378:metabolism; D016264:Dextran Sulfate / Q000494:pharmacology; D004926:Escherichia coli; D005341:Fibrinolysin / Q000378:metabolism; D005342:Fibrinolysis / Q000502:physiology*; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D010958:Plasminogen / Q000378:metabolism; D015849:Plasminogen Inactivators / Q000097:blood*; D012475:Salmonella; D012769:Shock / Q000097:blood* / Q000139:chemically induced; D013552:Swine; D013556:Swine, Miniature; D013755:Tetradecanoylphorbol Acetate; D010959:Tissue Plasminogen Activator / Q000097:blood*
D002863:Chromogenic Compounds; D008070:Lipopolysaccharides; D015849:Plasminogen Inactivators; D010958:Plasminogen; D016264:Dextran Sulfate; D010959:Tissue Plasminogen Activator; D005341:Fibrinolysin; D013755:Tetradecanoylphorbol Acetate