title: Effect of endothelin on angiotensin converting enzyme activity in cultured pulmonary artery endothelial cells.
abstract: We studied the effects of endothelin on angiotensin converting enzyme (ACE) in cultured pulmonary artery endothelial cells. ACE activity was increased 2.5-fold by the addition of 1 x 10(-8) mol/l endothelin-1. Endothelin-1 also stimulated calcium influx and phospholipase C activity in a dose-dependent manner. Calcium influx, phospholipase C and ACE activity were suppressed 60-70% in the presence of endothelin-1 (10(-10) to 10(-6) mol/l) by 50 microliters neomycin. These results suggest that ACE was stimulated by endothelin-1 and that its activity may be closely related to phosphatidylinositol turnover stimulated by endothelin-1.
authors.affiliation: Department of Cardiovascular Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kawaguchi
authors.forename: H
authors.initials: H
authors.lastname: Sawa
authors.forename: H
authors.initials: H
authors.lastname: Yasuda
mesh_terms: D000803:Angiotensin I / Q000378:metabolism; D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D016232:Endothelins / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D066298:In Vitro Techniques; D007703:Peptidyl-Dipeptidase A / Q000187:drug effects*; D011651:Pulmonary Artery / Q000166:cytology; D010738:Type C Phospholipases / Q000187:drug effects
chemical_list: D016232:Endothelins; D000804:Angiotensin II; D000803:Angiotensin I; D010738:Type C Phospholipases; D007703:Peptidyl-Dipeptidase A; D002118:Calcium