Effect of cortisol on testosterone production by immature pig Leydig cells.
The direct effects of hydrocortisone (HS) and adrenocorticotropin (ACTH) on testicular testosterone production were studied in purified immature pig Leydig cells in vitro. Leydig cells were obtained from 3- to 4-week-old piglet testes by enzymatical dispersion followed by discontinuous Percoll gradient centrifugation. Leydig cells were treated with HS and ACTH in the absence or presence of luteinizing hormone (LH) after 12 h of incubation. Media were collected 48 h later for testosterone and cyclic adenosine 3',5'-monophosphate (cAMP) measurement. Treatment of Leydig cells with increasing concentrations (0.001-10.0 micrograms/ml) of HS for 48 h resulted in a dose-dependent increase in basal and LH-stimulated testosterone production. Increasing duration (6-72 h) of treatment with HS (100 ng/ml) led to a time-dependent increase in basal and LH-stimulated testosterone production, achieving statistical significance by 48 and 24 h, respectively. HS increased LH-stimulated cAMP production. HS also increased testosterone production induced by (Bu)2 cAMP. Forskolin stimulated testosterone production to an extent comparable to that attained with LH, and HS augmented forskolin-stimulated testosterone production. HS enhanced the conversion of exogenous 17 alpha-hydroxyprogesterone to testosterone, but did not affect the conversion of pregnenolone and progesterone to testosterone, suggesting a specific stimulation of 17,20-desmolase. Porcine ACTH had no influence on basal and LH-stimulated testosterone production. These results suggest that HS directly stimulates immature pig Leydig cell steroidogenesis, at least in part via an enhancement of the generation of cAMP, leading to an increase in the activity of 17,20-desmolase.
Li
P S
PS
Department of Physiology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, R.O.C.
D019326:17-alpha-Hydroxyprogesterone; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000446:Aldehyde-Lyases / Q000378:metabolism; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology*; D006908:Hydroxyprogesterones / Q000378:metabolism; D066298:In Vitro Techniques; D007985:Leydig Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000494:pharmacology; D008297:Male; D011284:Pregnenolone / Q000378:metabolism; D013254:Steroid 17-alpha-Hydroxylase; D013552:Swine; D013739:Testosterone / Q000096:biosynthesis*
D006908:Hydroxyprogesterones; D005576:Colforsin; D013739:Testosterone; D019326:17-alpha-Hydroxyprogesterone; D011284:Pregnenolone; D000324:Adrenocorticotropic Hormone; D007986:Luteinizing Hormone; D003577:Cytochrome P-450 Enzyme System; D000242:Cyclic AMP; D013254:Steroid 17-alpha-Hydroxylase; D000446:Aldehyde-Lyases; D006854:Hydrocortisone