Formation of cyclosporins by Tolypocladium inflatum.
Submerged cultures of the low-production strain of Tolypocladium inflatum DSM 63544 formed a mixture of cyclosporins (CS) consisting of CS-A, CS-B, "CS-3" and "CS-4". Glucose, sucrose and maltose were highly favored for biomass production but provided a different physiological state necessary for CS biosynthesis. Not only the magnitude of CS production but also the proportion of individual components of the CS mixture were affected by the C source. Intensive CS synthesis was in correlation with the formation of CS-3. Lower yields of CS were accompanied by an increased proportion of CS-A in the CS complex. The best specific production of CS was achieved on the glucose medium, the highest yield of CS-A on the maltose medium. There was no remarkable relationship between the biomass formation and the intensity of CS synthesis.
Zhao
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DX
International Postgraduate Training Course UNESCO-ROSTE, Prague.
Beran
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Kozová
J
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Rehácek
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D000595:Amino Acid Sequence; D003470:Culture Media; D003524:Cyclosporins / Q000096:biosynthesis* / Q000737:chemistry; D007700:Kinetics; D003904:Mitosporic Fungi / Q000254:growth & development / Q000378:metabolism*; D008969:Molecular Sequence Data; D015394:Molecular Structure
D003470:Culture Media; D003524:Cyclosporins