title: A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types.
abstract: cDNA clones corresponding to an Mr approximately 80,000 receptor (type I receptor) for interleukin-1 (IL-1) have been isolated previously by mammalian expression. Here, we report the use of an improved expression cloning method to isolate human and murine cDNA clones encoding a second type (Mr approximately 60,000) of IL-1 receptor (type II receptor). The mature type II IL-1 receptor consists of (i) a ligand binding portion comprised of three immunoglobulin-like domains; (ii) a single transmembrane region; and (iii) a short cytoplasmic domain of 29 amino acids. This last contrasts with the approximately 215 amino acid cytoplasmic domain of the type I receptor, and suggests that the two IL-1 receptors may interact with different signal transduction pathways. The type II receptor is expressed in a number of different tissues, including both B and T lymphocytes, and can be induced in several cell types by treatment with phorbol ester. Both IL-1 receptors appear to be well conserved in evolution, and map to the same chromosomal location. Like the type I receptor, the human type II IL-1 receptor can bind all three forms of IL-1 (IL-1 alpha, IL-1 beta and IL-1ra). Vaccinia virus contains an open reading frame bearing strong resemblance to the type II IL-1 receptor.
authors.affiliation: Department of Molecular Biology, Immunex Research and Development Corporation, Seattle, WA 98101.
authors.forename: C J
authors.initials: CJ
authors.lastname: McMahan
authors.forename: J L
authors.initials: JL
authors.lastname: Slack
authors.forename: B
authors.initials: B
authors.lastname: Mosley
authors.forename: D
authors.initials: D
authors.lastname: Cosman
authors.forename: S D
authors.initials: SD
authors.lastname: Lupton
authors.forename: L L
authors.initials: LL
authors.lastname: Brunton
authors.forename: C E
authors.initials: CE
authors.lastname: Grubin
authors.forename: J M
authors.initials: JM
authors.lastname: Wignall
authors.forename: N A
authors.initials: NA
authors.lastname: Jenkins
authors.forename: C I
authors.initials: CI
authors.lastname: Brannan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D001483:Base Sequence; D005075:Biological Evolution; D015152:Blotting, Northern; D015139:Blotting, Southern; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002874:Chromosome Mapping; D003432:Cross-Linking Reagents; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012333:RNA, Messenger / Q000096:biosynthesis; D011869:Radioligand Assay; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000235:genetics*; D017472:Receptors, Interleukin-1
chemical_list: D003432:Cross-Linking Reagents; D007375:Interleukin-1; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D004247:DNA