Transient replication of BPV-1 requires two viral polypeptides encoded by the E1 and E2 open reading frames.
Bovine papillomavirus (BPV) DNA is maintained as an episome with a constant copy number in transformed cells and is stably inherited. To study BPV replication we have developed a transient replication assay based on a highly efficient electroporation procedure. Using this assay we have determined that in the context of the viral genome two of the viral open reading frames, E1 and E2, are required for replication. Furthermore we show that when produced from expression vectors in the absence of other viral gene products, the full length E2 transactivator polypeptide and a 72 kd polypeptide encoded by the E1 open reading frame in its entirety, are both necessary and sufficient for replication BPV in C127 cells.
Ustav
M
M
Cold Spring Harbor Laboratory, Cold Spring Harbor 11724.
Stenlund
A
A
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D015183:Restriction Mapping; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication
D004279:DNA, Viral; D014764:Viral Proteins