title: A possible mechanism of immunotherapy for patients with recurrent spontaneous abortions.
abstract: The mechanism of the beneficial effect of immunotherapy for human reproductive wastage remains to be elucidated. Induction of blocking antibodies such as anti-HLA class II antibodies and anti-idiotypic antibodies was investigated as the mechanism of specific immunosuppression in pregnancy. We reported the changes in the mixed lymphocyte reaction (MLR), T-cell subsets, and generation of anti-idiotypic antibodies after immunotherapy compared to before immunotherapy. MLR was significantly (P less than 0.001) inhibited after the immunization. The mean inhibition rate was 50.2%, suggesting that MLR blocking antibodies were induced by immunotherapy. Binding of autoantibodies to alloactivated maternal lymphoblasts against the paternal lymphocytes was detected in postimmunization cases in two-color flow-cytometric experiments. This suggests that anti-idiotypic antibodies were induced by the immunotherapy. The percentage of cytotoxic T-cells was significantly decreased (P less than 0.05) and the percentage of suppressor T-cells was significantly increased (P less than 0.01) after the immunotherapy, suggesting that a cell-mediated immune response was induced by the immunotherapy.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sugi
authors.forename: T
authors.initials: T
authors.lastname: Makino
authors.forename: T
authors.initials: T
authors.lastname: Maruyama
authors.forename: W K
authors.initials: WK
authors.lastname: Kim
authors.forename: R
authors.initials: R
authors.lastname: Iizuka
mesh_terms: D000026:Abortion, Habitual / Q000628:therapy*; D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007111:Immunity, Cellular; D007116:Immunization, Passive / Q000379:methods; D007167:Immunotherapy; D007958:Leukocyte Count; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D011247:Pregnancy; D013602:T-Lymphocytes, Cytotoxic; D050378:T-Lymphocytes, Regulatory
chemical_list: D000888:Antibodies, Anti-Idiotypic