Human papillomavirus type 16 transcripts expressed from viral-cellular junctions and full-length viral copies in CaSki cells and in a cervical carcinoma.
We have mapped using the RNA PCR the viral-cellular junctions of HPV16 viral-cellular cotranscripts expressed in CaSki cells and a cervical carcinoma to nt 3728 and 881, respectively. Both junctions were located within the E1-E2 region. Examination of the cellular sequences of the cotranscripts showed the presence of a polyadenylation signal in each of the transcripts. In CaSki cells and in the cervical carcinoma transcripts derived from the full-length early region including the E2 transcript were also detected. Our results suggest that the utilization of a cellular polyadenylation site could be important in the development of cancer by HPV.
Smits
H L
HL
Department of Medical Microbiology, University of Amsterdam, The Netherlands.
Cornelissen
M T
MT
Jebbink
M F
MF
van den Tweel
J G
JG
Struyk
A P
AP
Briët
M
M
ter Schegget
J
J
D001483:Base Sequence; D002277:Carcinoma / Q000235:genetics / Q000382:microbiology*; D002460:Cell Line; D005260:Female; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000382:microbiology*; D015678:Viral Structural Proteins / Q000235:genetics
D009841:Oligonucleotides; D012333:RNA, Messenger; D012367:RNA, Viral; D015678:Viral Structural Proteins