Antigen-induced changes in the lymphocyte-high endothelial venule interaction.
The sequential changes of some morphometric parameters (lymph node weight, high endothelial venule (HEV)-content) after an antigenic challenge (sheep red blood cell; SRBC) and the alterations of HEV-function (HEV-adhesiveness measured with a HEV binding assay) were studied in rat lymph nodes over a period of seven days with daily measurements. Authors suggest that the proper estimation of the HEV-content of a lymph node in a HEV binding assay allows to express the HEV-adhesiveness numerically, and to compare the data obtained by this assay quantitatively. The HEV-adhesiveness is the strongest on day 6 after the antigenic challenge, the early increase in weight on day 2 is attributed to an increased blood flow and the changes in the HEV-content are presumed to reflect a simple dilation of HEVs (on day 2) and later (on days 4 and 5) an increase in the height of high endothelial (HE) cells. A crucial role is ascribed to the altered HEV-adhesiveness in the increase of lymphocyte migration to the challenged lymph nodes.
Csanaky
G
G
Department of Pathology, University Medical School of Pécs, Hungary.
Kalász
V
V
Pap
T
T
D000818:Animals; D000941:Antigens / Q000276:immunology*; D002448:Cell Adhesion; D002465:Cell Movement; D004730:Endothelium, Vascular / Q000276:immunology / Q000502:physiology*; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D008198:Lymph Nodes / Q000502:physiology*; D008214:Lymphocytes / Q000276:immunology / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D000941:Antigens