Characteristics of Staphylococcus aureus isolated from lesions of horses.
Seventy-six Staphylococcus aureus strains isolated from various lesions of horses were characterized. All of the 76 strains were identified as biotypes B (38.2%) and C (61.8%). Of 55 strains tested, 42 (76.4%) were differentiated into 7 coagulase types. Coagulase types V and VII were predominant in the metritis strains. Coagulase type II was found most frequently in the strains from phlegmon, dermatitis, sinusitis, empyema sinus, and nasal catarrh. Forty-two (55.3%) of the 76 strains were differentiated into 24 phage patterns. Twenty (58.8%) of 34 typable strains from metritis were lysed by the human group I phage 52, and group II phages 3A, 3C, 55 and 71. Forty-five (59.2%) of the 76 strains were resistant to 1 or more of 6 antibiotics. Strains resistant to penicillin G, irrespective of source, were most frequent (95.6%). Forty (93.0%) of 43 strains resistant to penicillin G alone or in combination with other antibiotics produced beta-lactamase. Only 8 (10.5%) of the 76 strains produced enterotoxins A (n = 2), B (n = 1) or C (n = 5), and they all were isolated from metritis. Only 1 strain isolated from phlegmon and 2 from metritis produced exfoliative toxin (ET) and toxic shock syndrome toxin-1 (TSST-1), respectively. The latter 2 strains also produced enterotoxin C. The results of the present study showed the first evidence of the presence of both ET- and TSST-1-producing S. aureus isolated from horses.
Shimizu
A
A
Department of Animal Hygiene, Faculty of Agriculture, Kobe University, Hyogo, Japan.
Kawano
J
J
Ozaki
J
J
Sasaki
N
N
Kimura
S
S
Kamada
M
M
Anzai
S
S
Saito
H
H
Sato
H
H
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D001434:Bacteriophage Typing; D003030:Coagulase / Q000096:biosynthesis; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary; D004352:Drug Resistance, Microbial; D004716:Endometritis / Q000382:microbiology / Q000662:veterinary; D004768:Enterotoxins / Q000096:biosynthesis; D005260:Female; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000662:veterinary; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013207:Staphylococcal Skin Infections / Q000382:microbiology / Q000662:veterinary; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000201:enzymology; D001618:beta-Lactamases / Q000096:biosynthesis
D000900:Anti-Bacterial Agents; D003030:Coagulase; D004768:Enterotoxins; D001618:beta-Lactamases