Expression of exogenous DNA in rat liver cells after liposome-mediated transfection in vivo.
Liposomes containing the expression vector pRSVneo coding for neomycin phosphotransferase-II were used for a gene transfer into rat liver cells in vivo. After intravenous application of liposomes to male Wistar-rats, non-integrated vector DNA was detected by blot-hybridisation in isolated nuclei of hepatocytes. Presence of messenger RNA specific for the transferred construct was demonstrated by Northern analyses of poly(A)+RNA. The biological activity of the neomycin phosphotransferase-II gene and its enzyme could be demonstrated in hepatocytes for at least 7d post injection. Liposome-mediated gene transfer into adult animals is discussed as a model system to study the expression and fate of transferred genes in adult rat hepatocytes under physiological conditions.
Leibiger
B
B
Institute of Biochemistry, School of Medicine, Ernst Mortiz Arndt University, Greifswald, Germany.
Leibiger
I
I
Sarrach
D
D
Zühlke
H
H
D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol; D004247:DNA / Q000008:administration & dosage / Q000235:genetics*; D004337:Drug Carriers; D005821:Genetic Techniques; D019868:Kanamycin Kinase; D008081:Liposomes; D008099:Liver / Q000378:metabolism*; D008297:Male; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010770:Phosphotransferases / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping; D014162:Transfection
D004337:Drug Carriers; D008081:Liposomes; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D004247:DNA; D002784:Cholesterol; D010770:Phosphotransferases; D019868:Kanamycin Kinase