FVB/N: an inbred mouse strain preferable for transgenic analyses.
FVB/N mice offer a system suitable for most transgenic experiments and subsequent genetic analyses. The inbred FVB/N strain is characterized by vigorous reproductive performance and consistently large litters. Moreover, fertilized FVB/N eggs contain large and prominent pronuclei, which facilitate microinjection of DNA. The phenotype of large pronuclei in the zygote is a dominant trait associated with the FVB/N oocyte but not the FVB/N sperm. In experiments to generate transgenic mice, the same DNA constructs were injected into three different types of zygotes: FVB/N, C57BL/6J, and (C57BL/6J x SJL/J)F1. FVB/N zygotes survived well after injection, and transgenic animals were obtained with efficiencies similar to the F1 zygotes and much better than the C57BL/6J zygotes. Genetic markers of the FVB/N strain have been analyzed for 44 loci that cover 15 chromosomes and were compared with those of commonly used inbred strains. In addition to the albino FVB/N strain, pigmented congenic strains of FVB/N are being constructed. These features make the FVB/N strain advantageous to use for research with transgenic mice.
Taketo
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Jackson Laboratory, Bar Harbor, ME 04609.
Schroeder
A C
AC
Mobraaten
L E
LE
Gunning
K B
KB
Hanten
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G
Fox
R R
RR
Roderick
T H
TH
Stewart
C L
CL
Lilly
F
F
Hansen
C T
CT
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002678:Chimera; D005260:Female; D005298:Fertility; D005814:Genes, Viral; D005819:Genetic Markers; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D013539:Simian virus 40
D005819:Genetic Markers; D015345:Oligonucleotide Probes