title: Nucleotide sequences of the 3' leader and 5' trailer regions of human respiratory syncytial virus genomic RNA.
abstract: The nucleotide sequences of the 3' extracistronic (leader) and 5' extracistronic (trailer) regions were determined for genomic RNA (vRNA) of human respiratory syncytial virus (RSV) strain A2. To sequence the 3' leader region, vRNA was extracted from purified virions, size-selected, polyadenylated, copied into cDNA, amplified by the polymerase chain reaction, cloned, and sequenced. The 3' leader sequence is 44 nt, which is somewhat shorter than its counterparts (50 to 70 nt) in other nonsegmented negative-strand viruses sequenced to date. The 5' trailer region was mapped and sequenced in part directly by dideoxynucleotide sequencing of vRNA. The sequence was confirmed and completed by analysis of cDNA clones derived from vRNA. The 5' trailer sequence is 155 nt in length, which is substantially longer than its counterparts (40 to 70 nt) in other nonsegmented negative-strand viruses. Ten of the 11 terminal nt of the 3' leader and 5' trailer regions were complementary. Among the other paramyxoviruses, the terminal 5 to 16 nt of the leader and trailer regions are highly conserved, but the corresponding RSV sequences were identical to the others only for the terminal 2 nt of each end. Surprisingly, the termini of the RSV leader and trailer regions were in somewhat better agreement with those of the rhabdoviruses vesicular stomatitis virus and rabies virus, sharing identity for the first 3 or 4 nt.
authors.affiliation: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: M A
authors.initials: MA
authors.lastname: Mink
authors.forename: D S
authors.initials: DS
authors.lastname: Stec
authors.forename: P L
authors.initials: PL
authors.lastname: Collins
mesh_terms: D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D006801:Humans; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010252:Paramyxoviridae / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D012136:Respiratory Syncytial Viruses / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012367:RNA, Viral