Primed and naive helper T cells in labial glands from patients with Sjogren's syndrome.
This study has investigated the presence and distribution of B cells, T cells and T-cell subsets within labial glands of patients with primary Sjogren's syndrome (n = 9) and secondary Sjogren's syndrome associated with rheumatoid arthritis (n = 8) using a sequential double immunoperoxidase technique and true colour image analysis. The composition of the inflammatory infiltrates was similar in glands from both patient groups. B cells were normally present within large foci with few detected in diffuse infiltrates such that the ratio of T:B cells in foci (2.4:1) was significantly lower than in diffuse infiltrates (7.3:1; P less than 0.001). In all infiltrates helper T cells (CD8-, CD3+) predominated over suppressor/cytotoxic cells (CD8+, CD3+; 2.7:1). Analysis of primed (CD45RA-, CD45RO+) and naive (CD45RA+, CD45RO-) CD8- T cells showed that the ratio of the primed to naive subset was significantly higher in focal (4.2:1) compared to diffuse (1.5:1; P less than 0.001) areas of lymphoid infiltration. These results indicate that the focal lymphocytic infiltrates characteristic of Sjogren's syndrome contain B cells associated with a T-cell population consisting predominantly of primed CD8- helper T cells. This latter population may be responsible for upregulating glandular B-cell activity in Sjogren's syndrome.
Matthews
J B
JB
Department of Oral Surgery, University of Birmingham, UK.
Deacon
E M
EM
Kitas
G D
GD
Salmon
M
M
Potts
A J
AJ
Hamburger
J
J
Bacon
P A
PA
D015703:Antigens, CD / Q000032:analysis; D001402:B-Lymphocytes / Q000473:pathology; D016827:CD8 Antigens / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D012469:Salivary Glands / Q000276:immunology / Q000473:pathology*; D012859:Sjogren's Syndrome / Q000276:immunology / Q000473:pathology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000473:pathology*
D015703:Antigens, CD; D016827:CD8 Antigens