Eicosanoid release and early changes in two acute non specific inflammatory reactions. Major role of prostacyclin and leukotrienes.
We have compared the early development (0-4h) of two acute non-specific inflammatory reactions induced by the intrapleural injection of isologous serum or a suspension of CaPP crystals. The intensity of the reactions was assessed in terms of the exudate volume, the number and ratio of pleural cells and different cell functions and secretions. The number of exudative cells elicited by isologous serum was higher than with CaPP but the PMN/Monocytes ratio was the same. The amount of protein in the serum-induced exudate was constant from 1 h to 4 h and was similar in the CaPP-induced pleural exudate at the latter time. The amount of complement increased similarly in the two models. The chemotactic potency of the exudates and cell supernatants following incubation showed similar values in the two models. Eicosanoid levels were higher in CaPP--than in isologous serum-induced exudates. Prostacyclin and peptidoleukotrienes were released in specially large amounts at the very outset of the inflammatory reactions.
Tissot
M
M
Département de Pharmacologie, CNRS URA 595, Hôpital Cochin, Paris, France.
Pham Huy
D
D
Roch-Arveiller
M
M
Mathieu
J
J
Giroud
J P
JP
D000208:Acute Disease; D000818:Animals; D002130:Calcium Phosphates / Q000494:pharmacology; D002478:Cells, Cultured; D015777:Eicosanoids / Q000378:metabolism*; D011464:Epoprostenol / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D007975:Leukotriene B4 / Q000502:physiology; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D010998:Pleurisy / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013189:SRS-A / Q000502:physiology; D013929:Thromboxane B2 / Q000378:metabolism
D002130:Calcium Phosphates; D015777:Eicosanoids; D013189:SRS-A; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; D007975:Leukotriene B4; D013929:Thromboxane B2; D009240:N-Formylmethionine Leucyl-Phenylalanine; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; D011464:Epoprostenol; C485829:calcium phosphate, dibasic, anhydrous