title: [Occurrence of anti-Mycobacterium antibodies detected by the indirect immunofluorescence method in patients with pulmonary tuberculosis and in healthy controls].
abstract: Due to an unfavorable epidemiological situation in tuberculosis in Poland and diagnostical difficulties the authors suggest the need to enlarge the diagnostical tests used in detecting M. tuberculosis with modern immunological methods. In this study we analysed serum of patients with pulmonary tuberculosis determining with the indirect immunofluorescence method the levels of specific antibodies of the IgG class directed against Mycobacteria. The heat attenuated H37Rv strain was used as a standard. Significantly higher levels of antibodies were seen in patients with tuberculosis in comparison with control. These preliminary results suggest diagnostic utility of antimycobacterial antibodies assessed with immunofluorescence in diagnosing infections and suggest the possible comparability with the ELISA method.
authors.affiliation: Katedry i Zakładu Mikrobiologii i Immunologii Pomorskiej Akademii Medycznej, Szczecinie.
authors.forename: A
authors.initials: A
authors.lastname: Kaczmarek
authors.forename: J
authors.initials: J
authors.lastname: Hałasa
authors.forename: M
authors.initials: M
authors.lastname: Kamiński
authors.forename: M
authors.initials: M
authors.lastname: Nowak
authors.forename: J
authors.initials: J
authors.lastname: Bugaj
mesh_terms: D000907:Antibodies, Bacterial / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D012016:Reference Values; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G
vernacular_title: Wystepowanie przeciwciał przeciwpratkowych wykrywanych metoda immunofluorescencji pośredniej u chorych na gruźlice płuc i kontrolnie u ludzi zdrowych.