title: Regulation of T-cell sensitization at epithelial surfaces in the respiratory tract: suppression of IgE responses to inhaled antigens by CD3+ Tcr alpha-/beta- lymphocytes (putative gamma/delta T cells).
abstract: Repeated exposure of Brown Norway rats to an aerosol of ovalbumin (OVA) induced a state of antigen-specific immunological tolerance, particularly in the IgE isotype. Tolerance was transferable to naive syngeneic animals by inoculation of splenic T cells from tolerant rats. Sequential depletion of tolerant spleen cells by sorting techniques prior to adoptive transfer, employing T-cell subset-specific monoclonal antibodies, indicated that the cells mediating tolerance were CD3+, CD4-, CD5+ and CD8+, but lacked alpha or beta chains in the T-cell receptor (TcR), suggesting that they may be part of the gamma/delta T-cell lineage. Consistent with this suggestion, the sorted population demonstrated considerable enrichment for TcR gamma chain-specific mRNA. As few as 2 x 10(3) cells are sufficient to adoptively transfer tolerance in 200-g adult rats in this model.
authors.affiliation: Division of Cell Biology, Western Australian Research Institute for Child Health, Princess Margaret Hospital, Subiaco.
authors.forename: C
authors.initials: C
authors.lastname: McMenamin
authors.forename: J
authors.initials: J
authors.lastname: Oliver
authors.forename: B
authors.initials: B
authors.lastname: Girn
authors.forename: B J
authors.initials: BJ
authors.lastname: Holt
authors.forename: U R
authors.initials: UR
authors.lastname: Kees
authors.forename: W R
authors.initials: WR
authors.lastname: Thomas
authors.forename: P G
authors.initials: PG
authors.lastname: Holt
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D017252:CD3 Complex; D007108:Immune Tolerance / Q000276:immunology*; D007073:Immunoglobulin E / Q000096:biosynthesis*; D010047:Ovalbumin / Q000008:administration & dosage; D051381:Rats; D011914:Rats, Inbred BN; D011919:Rats, Inbred Strains; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D012137:Respiratory System / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D011948:Receptors, Antigen, T-Cell; D016692:Receptors, Antigen, T-Cell, gamma-delta; D007073:Immunoglobulin E; D010047:Ovalbumin