Herpes simplex virus type 1 and type 2 infection in human oral mucosa in culture.
To examine the sensitivity of human oral mucosa to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) infection, human gingival mucosa explants were infected with either HSV-1 or HSV-2 in vitro and the expression of virus specific antigen was examined by the immunofluorescent antibody technique. HSV-2 antigen was found in the basement membrane, basal cell layer and lower prickle cell layer. This finding was consistent with the HSV-1 infection. Electron microscopic study revealed the presence of nucleocapsids and enveloped virus particles in the basal cells of HSV-2-infected organ cultures. These findings indicate that human gingival mucosa is sensitive to infection with HSV-2, as well as HSV-1, and that the virus may replicate in the undifferentiated epithelial cells of mucosal epithelium.
Yura
Y
Y
Second Department of Oral and Maxillofacial Surgery, Tokushima University School of Dentistry, Japan.
Iga
H
H
Kondo
Y
Y
Harada
K
K
Yanagawa
T
T
Yoshida
H
H
Sato
M
M
D000293:Adolescent; D000328:Adult; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D002648:Child; D046508:Culture Techniques; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D009061:Mouth Mucosa / Q000382:microbiology*; D018139:Simplexvirus / Q000648:ultrastructure; D013283:Stomatitis, Herpetic / Q000382:microbiology*; D014771:Virion / Q000648:ultrastructure
D000956:Antigens, Viral