title: Endothelial cells express the interleukin-1 receptor type I.
abstract: Interleukin-1 (IL-1) profoundly affects a number of functions of vascular cells. Two distinct IL-1 receptors (IL-1R) are expressed on different cell types: the 80 Kd IL-1RI on T cells and fibroblasts, and the 68 Kd IL-1RII on B cells and myelomonocytic cells. The presence and functionality of IL-1R on vascular cells has been investigated by using polyomatransformed mouse endothelial cell (EC) lines (sEnd.1 and tEnd.1). These cells expressed specific and saturable binding sites for IL-1 (1,273 sites per cell with kd 9.5 x 10(-11) mol/L for sEnd.1, and 771 sites per cell with kd 8.5 x 10(-11) mol/L for tEnd.1, with radioiodinated IL-1 alpha as ligand). Binding of IL-1 was also evident at single cell level by autoradiography. By cross-linking studies, the molecular weight of the IL-1 binding protein on EC was approximately 80 Kd. This was confirmed by the presence in EC of mRNA for the 80 Kd IL-1RI. The IL-1RI on EC was apparently functional, since EC responded to IL-1 with IL-6 mRNA expression and IL-6 bioactivity production. These results were extended to human EC and vascular smooth muscle cells, which were also found to express mRNA for IL-1RI.
authors.affiliation: Sclavo Research Center, Siena, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Boraschi
authors.forename: A
authors.initials: A
authors.lastname: Rambaldi
authors.forename: A
authors.initials: A
authors.lastname: Sica
authors.forename: P
authors.initials: P
authors.lastname: Ghiara
authors.forename: F
authors.initials: F
authors.lastname: Colotta
authors.forename: J M
authors.initials: JM
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: de Rossi
authors.forename: C
authors.initials: C
authors.lastname: Zoia
authors.forename: G
authors.initials: G
authors.lastname: Remuzzi
authors.forename: F
authors.initials: F
authors.lastname: Bussolino
mesh_terms: D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D002460:Cell Line; D004730:Endothelium, Vascular / Q000378:metabolism*; D006390:Hemangioendothelioma / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis; D011971:Receptors, Immunologic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins