A monoclonal antibody distinguishes two types of phosphatidylinositol 4-kinase.
A monoclonal antibody has been developed against the type II PtdIns 4-kinase from bovine brain. This antibody, 4C5G, causes greater than 90% inhibition of the type II PtdIns 4-kinase from bovine brain, rat brain and human erythrocytes. However, it fails to inhibit type III PtdIns 4-kinase from bovine brain or PtdIns 3-kinase from rat liver. These results suggest that type II and type III PtdIns 4-kinases are distinct gene products, and that 4C5G will be useful in studying the function of the type II PtdIns 4-kinase.
Endemann
G C
GC
Department of Cell Biology, Stanford University School of Medicine, CA 94305.
Graziani
A
A
Cantley
L C
LC
D019870:1-Phosphatidylinositol 4-Kinase; D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000201:enzymology; D002417:Cattle; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D008099:Liver / Q000201:enzymology; D010063:Ovum / Q000201:enzymology; D010770:Phosphotransferases / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000302:isolation & purification; D051381:Rats; D014982:Xenopus laevis
D000911:Antibodies, Monoclonal; D010770:Phosphotransferases; D019870:1-Phosphatidylinositol 4-Kinase