[Post-translational regulation of N-glycosylated proteins expression in human intestinal cells in culture].
HT-29 cells derived from a human colonic adenocarcinoma, can express a typical intestinal differentiation. Undifferentiated HT-29 cells accumulate N-linked glycoproteins substituted with unprocessed carbohydrate chains before to degrade them. Conversely, carbohydrate chains of N-linked glycoproteins are classically processed in differentiated HT-29 cells. The instability of N-linked glycoproteins in undifferentiated HT-29 cells is due to their rapid delivery from the endoplasmic reticulum to a compartment with lysosomal characteristics. This catabolitic pathway involves a bypass of the Golgi apparatus.
Ogier-Denis
E
E
Unité INSERM 180, UFR Biomédicale des Saints-Pères, Paris.
Sapin
C
C
Bauvy
C
C
Hourri
J J
JJ
Aubery
M
M
Trugnan
G
G
Codogno
P
P
D000230:Adenocarcinoma / Q000473:pathology*; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000473:pathology*; D004355:Drug Stability; D006023:Glycoproteins / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007976:Leupeptins / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000378:metabolism
D006023:Glycoproteins; D007976:Leupeptins; D011134:Polysaccharides
Régulation post-traductionnelle de l'expression des protéines N-glycosylées dans des cellules intestinales humaines en culture.