Adipogenesis inhibitory factor. A novel inhibitory regulator of adipose conversion in bone marrow.
Recombinant adipogenesis inhibitory factor (AGIF) was purified to homogeneity from the conditioned medium of COS-1 cells transfected with human AGIF cDNA. The amino-terminal sequence analysis of the mature AGIF revealed that AGIF was produced as a precursor consisting of 199 amino acids and processed into a mature form of 178 amino acids by a cleavage between Ala(-1) and Pro(+1). The purified AGIF inhibited the process of adipogenesis in mouse 3T3-L1 preadipocytes, indicating that AGIF directly acts on the cells. AGIF acted as an adipogenic antagonist not only on the extramedullary cell line 3T3-L1 but also on the mouse bone marrow stroma-derived cell line H-1/A, suggesting that this cytokine may regulate adipogenesis in bone marrow.
Ohsumi
J
J
Bioscience Research Laboratories, Sankyo Co. Ltd., Tokyo, Japan.
Miyadai
K
K
Kawashima
I
I
Ishikawa-Ohsumi
H
H
Sakakibara
S
S
Mita-Honjo
K
K
Takiguchi
Y
Y
D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D001853:Bone Marrow / Q000187:drug effects* / Q000378:metabolism; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D016207:Cytokines / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D017370:Interleukin-11; D007378:Interleukins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012689:Sequence Homology, Nucleic Acid
D016207:Cytokines; D017370:Interleukin-11; D007378:Interleukins; D011994:Recombinant Proteins