[Monoclonal antibodies to cyclodextrin-glycosyltransferase from Bacillus macerans].
Cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) was isolated from the cell-free culture medium of Bacillus macerans strain BKMB-506. The purified enzyme was used to immunize BALB/c mice. Polyclonal antibodies (PAbs) and 15 hybridoma cells producing monoclonal antibodies (MAbs) against CGTase were obtained. The properties of MAbs were studied using ELISA and immunoblotting. A method for detecting small amounts of CGTase was developed. The interactions of PAbs and MAbs with CGTase of Bac. circulans var. alkalophilis were investigated.
Arbatova
E
E
Reében
M
M
Kestner
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D001407:Bacillus / Q000201:enzymology; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005964:Glucosyltransferases / Q000276:immunology*; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase
Monoklonal'nye antitela k tsiklodekstrin-gliukanotransferaze Bacillus macerans.