Functional alteration of macrophages by a slime-forming Lactococcus lactis ssp. cremoris.
The effect of a slime-forming, encapsulated Lactococcus lactis ssp. cremoris KVS20 on macrophage function has been examined in vivo and in vitro in short-term studies. Peritoneal macrophages in which 21 to 34% of macrophage was presenting Fc gamma-receptor positive macrophages were elicited by intraperitoneal injection of 10 to 50 mg/kg of L. lactis ssp. cremoris KVS20. The peritoneal macrophage exhibited cytotoxic activity against Sarcoma-180 cells in which the maximum activity was obtained in macrophage from mice injected with 10 mg/kg on d 5. However, L. lactis ssp. cremoris KVS20 rendered the elicited macrophage cytotoxic in vitro. The cytotoxicity was significantly augmented by 6- and 24-h treatment at the concentration of 50 to 500 micrograms/ml. These results obtained in the short-term studies demonstrated that the antitumor activity of L. lactis ssp. cremoris KVS20 may be mediated through the enhanced cytotoxic activity of macrophage.
Kitazawa
H
H
Laboratory of Animal Products Chemistry, Laboratory of Animal Morphology, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Nomura
M
M
Itoh
T
T
Yamaguchi
T
T
D000818:Animals; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D013294:Lactococcus lactis / Q000502:physiology*; D008264:Macrophages / Q000276:immunology / Q000382:microbiology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010529:Peritoneal Cavity / Q000166:cytology / Q000382:microbiology; D010587:Phagocytosis; D012510:Sarcoma 180; D013047:Specific Pathogen-Free Organisms; D014407:Tumor Cells, Cultured