Genetic typing using automated electrophoresis and fluorescence detection.
Multi-color fluorescence detection systems offer unique advantages when compared to single label detection methods for DNA typing, genetic disease testing, population fingerprinting, and DNA mapping. Internal controls are easily used and identified by different color dye labels. Multiple independent samples or multiple analyses of the same sample are run in each lane of a gel. Precision of size assignment and quantification are improved. Here, we will review a variety of methods used to analyze DNA and present the advantages of the multi-color fluorescence dye approach. An automated and quantitative DNA typing assay for human identification is shown. This method is an improvement over previous manual techniques and uses multi-color fluorescence labeling, electrophoresis and real-time detection methodology.
Robertson
J
J
Forensic Science Research and Training Section, FBI Laboratory, Quantico, Virginia.
Ziegle
J
J
Kronick
M
M
Madden
D
D
Budowle
B
B
D000703:Analog-Digital Conversion; D004247:DNA / Q000032:analysis*; D004586:Electrophoresis; D005456:Fluorescent Dyes; D006801:Humans; D009136:Muscular Dystrophies / Q000235:genetics; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
D005456:Fluorescent Dyes; D004247:DNA