title: Structural architecture of an outer membrane channel as determined by electron crystallography.
abstract: Porins are a family of membrane channels commonly found in the outer membranes of Gram-negative bacteria where they serve as diffusional pathways for waste products, nutrients and antibiotics, and can also be receptors for bacteriophages. Porin channels have been shown in vitro to be voltage-gated. They can exhibit slight selectivities for certain solutes; for example PhoE porin has some selectivity for anionic and phosphate-containing compounds. Unlike many known membrane proteins which often contain long stretches of hydrophobic segments that are believed to traverse the membrane in a helical conformation, porins are found to have charged residues distributed almost uniformly along their primary sequences and have most of their secondary structure in a beta-sheet conformation. We have made crystalline patches of PhoE porin embedded in a lipid bilayer and have used these to determine the structure of PhoE porin by electron crystallography to a resolution of 6A. The basic structure consists of a trimer of elliptically shaped, cylindrical walls of beta sheet. Each cylinder has an inner lining, formed by parts of the polypeptide, that defines the channel size. The structure provides a clue as to how deletions of segments of polypeptide, which are found in certain mutants, can result in an actual increase in the channel size.
authors.affiliation: Donner Laboratory, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.
authors.forename: B K
authors.initials: BK
authors.lastname: Jap
authors.forename: P J
authors.initials: PJ
authors.lastname: Walian
authors.forename: K
authors.initials: K
authors.lastname: Gehring
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000648:ultrastructure*; D003461:Crystallography; D015640:Ion Channel Gating; D008051:Lipid Bilayers; D018272:Porins; D011487:Protein Conformation; D014961:X-Ray Diffraction
chemical_list: D001425:Bacterial Outer Membrane Proteins; D008051:Lipid Bilayers; D018272:Porins