title: Relative role of bone and kidney in the hypercalcaemia associated with the rat Walker carcinosarcoma 256.
abstract: The rat Walker carcinosarcoma 256 is an animal model for humoral hypercalcaemia of malignancy (HHM). In this model, the relative contribution of bone and kidney in the hypercalcaemia of tumour-bearing rats was investigated. Daily administration of pamidronate, a bone resorption inhibitor, for 2 days prevented the increased fasting Ca2+ excretion observed in the hypercalcaemic rats, although serum Ca2+ remained high. However, the high serum Ca2+ normalised after the acute injection of ethiofos, an inhibitor of renal Ca2+ reabsorption, which was associated with a marked increase of Ca2+ excretion. Changes in Ca2+ were accompanied by similar changes in Mg2+. The results indicate that altered renal Ca2+ handling has a key role in the hypercalcaemia associated with this HHM model.
authors.affiliation: Laboratorio de la Unidad Metabólica, Fundación Jiménez Diaz-C.S.I.C., Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Hurtado
authors.forename: P
authors.initials: P
authors.lastname: Esbrit
authors.forename: A
authors.initials: A
authors.lastname: Rapado
mesh_terms: D004999:Amifostine / Q000627:therapeutic use; D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002279:Carcinoma 256, Walker / Q000150:complications*; D004164:Diphosphonates / Q000627:therapeutic use; D005260:Female; D006934:Hypercalcemia / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D000077268:Pamidronate; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004164:Diphosphonates; D004999:Amifostine; D000077268:Pamidronate; D002118:Calcium