MHC class II-restricted T-cell hybridomas recognizing the nucleocapsid protein of avian coronavirus IBV.
Mice were immunized with purified infectious bronchitis virus (IBV), strain M41. Spleen cells, expanded in vitro by stimulation with M41, were immortalized by fusion to obtain T-cell hybridomas, and two major histocompatability complex (MHC) class II (I-E)-restricted T-cell hybridomas were selected with specificity for IBV. Both hybridomas selectively recognized the internal nucleocapsid protein. The responses to 12 different strains of IBV varied markedly. This demonstrates antigenic variation of the nucleocapsid protein in addition to the known variation of the surface glycoprotein S.
Boots
A M
AM
Institute of Molecular Biology and Medical Biotechnology, University of Utrecht, The Netherlands.
Van Lierop
M J
MJ
Kusters
J G
JG
Van Kooten
P J
PJ
Van der Zeijst
B A
BA
Hensen
E J
EJ
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D002213:Capsid / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D001351:Infectious bronchitis virus / Q000276:immunology*; D007376:Interleukin-2 / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*; D014758:Viral Core Proteins / Q000276:immunology*
D000956:Antigens, Viral; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D014758:Viral Core Proteins