Transfection of lipoma cells with papilloma bovine virus subgenomic fragment.
Lipoma cells with consistent chromosomal aberration have been transfected with plasmids carrying papilloma bovine virus subgenomic fragment (PBV 69). The successful transformation of the cells was ascerted on the changed growth pattern of the cells in liquid medium, colony formation in soft agar and modified cell appearance in electron microscopy; transfection with PBV 69 has not been, however, sufficient to immortalize lipoma cells.
Haus
O
O
Department of Histology, Faculty of Medicine, University of Burgundy, Dijon, France.
Moisant
M
M
Racine
L
L
Zahnd
J P
JP
Padieu
P
P
Turc-Carel
C
C
D000273:Adipose Tissue / Q000473:pathology; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D002454:Cell Differentiation; D002455:Cell Division; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002869:Chromosome Aberrations; D002999:Clone Cells; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005347:Fibroblasts; D006801:Humans; D008067:Lipoma / Q000235:genetics / Q000473:pathology*; D008854:Microscopy, Electron; D009132:Muscles / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured