Myristylation alters DNA-binding activity and transactivation of FBR (gag-fos) protein.
FBR murine sarcoma virus (gag-fos) protein, a virally transduced Fos protein, exhibits decreased gene transactivation in comparison with the cellular Fos protein. Biochemical analysis suggests that myristylation of the virally encoded N-terminal gag region results in decreased DNA binding and transcriptional activation without affecting heterodimerization with Jun protein. These findings demonstrate that protein myristylation can modulate gene regulation by a DNA-binding protein.
Kamata
N
N
Department of Cell Biology, Vanderbilt University, Nashville, Tennessee 37232.
Jotte
R M
RM
Holt
J T
JT
D000595:Amino Acid Sequence; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D002678:Chimera; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015681:Fusion Proteins, gag-onc / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism; D015345:Oligonucleotide Probes; D016761:Oncogene Proteins v-fos; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos; D009053:Sarcoma Viruses, Murine / Q000235:genetics*; D013724:Teratoma; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D004268:DNA-Binding Proteins; D015681:Fusion Proteins, gag-onc; D009227:Myristic Acids; D015345:Oligonucleotide Probes; D016761:Oncogene Proteins v-fos; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; C067019:gag-fos protein, FBR murine sarcoma virus; D019814:Myristic Acid; D015500:Chloramphenicol O-Acetyltransferase; D011505:Protein-Tyrosine Kinases; D001616:beta-Galactosidase