beta-N-acetylhexosaminidases in human cerebrospinal fluid and serum of patients with multiple sclerosis.
Beta-N-Acetylhexosaminidase activity and isoenzyme have been investigated in normal human cerebrospinal fluid and that of patients with multiple sclerosis. beta-N-acetylhexosaminidase activity in normal cerebrospinal fluids has been resolved into five components. The major component was in a form that eluted from DEAE cellulose at the same salt concentration as hexosaminidase As, the isoenzyme previously identified in human serum. Cerebrospinal fluid from patients exhibited a different isoenzyme profile, showing a remarkable increase in a form having a pI which was more acidic than that of As. These changes have a potential use in the diagnosis and further biochemical characterization of multiple sclerosis.
Datti
A
A
Departmento di Medicina Sperimentale e Scienze, Biochimiche, University of Perugia, Italy.
Emiliani
C
C
Capocchi
G
G
Orlacchio
A
A
D002852:Chromatography, Ion Exchange; D005069:Evaluation Studies as Topic; D006801:Humans; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D007527:Isoenzymes / Q000097:blood* / Q000134:cerebrospinal fluid*; D009103:Multiple Sclerosis / Q000201:enzymology*; D001619:beta-N-Acetylhexosaminidases / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000302:isolation & purification
D007527:Isoenzymes; D001619:beta-N-Acetylhexosaminidases