The influence of extracellular magnesium on cell damage induced by ATP depletion in human fibroblasts.
Magnesium is an essential ion for the structural and functional integrity of cells. We have examined whether a low extracellular magnesium concentration contributes to cell damage induced by ATP depletion in a model system of quiescent fibroblasts. Cell damage assessed by the release of lactate dehydrogenase was slightly, but significantly increased in the absence of Mg2+. The increased cell damage may be caused by an increased Mg2+ loss during ATP depletion, by an altered cell membrane permeability for potassium and sodium, or by a disturbed balance between Mg2+ and Ca2+.
Kristensen
S R
SR
Department of Clinical Chemistry, Odense University Hospital, Denmark.
Hørder
M
M
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002463:Cell Membrane Permeability; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007460:Iodoacetamide / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology*; D010042:Ouabain / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
D000001:Calcimycin; D010042:Ouabain; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D000254:Sodium-Potassium-Exchanging ATPase; D008274:Magnesium; D002118:Calcium; D007460:Iodoacetamide