Oligonucleotides with bis-pyrene adduct in the backbone: syntheses and properties of intramolecular excimer forming probe.
2-(N-bis(2-pyrenylethyl)methyl-amino)ethanol (BPAE) was synthesized and its interaction with DNA was examined to explore the efficiency of intramolecular excimer forming probe. As a result the fluorescence of intramolecular excimer of BPAE disappeared with the addition of poly(dA)-poly(dT). This result suggests an unique type of probe with its introduction to oligonucleotide.
Kitamura
M
M
Division of Molecular Engineering, Graduate School of Engineering, Kyoto University, Japan.
Nimura
A
A
Yamana
K
K
Shimidzu
T
T
D004247:DNA / Q000032:analysis* / Q000737:chemistry; D004983:Ethanolamines / Q000138:chemical synthesis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D015345:Oligonucleotide Probes / Q000138:chemical synthesis* / Q000737:chemistry; D011721:Pyrenes / Q000138:chemical synthesis* / Q000737:chemistry
D004983:Ethanolamines; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011721:Pyrenes; C077798:2-(N-bis(2-pyrenylethyl)methylamino)ethanol; D004247:DNA