Comparison of different vascular prostheses and matrices in relation to endothelial seeding.
Thin-walled expanded polytetrafluoroethylene (ePTFE), woven Dacron and gelatin-impregnated Dacron (Gelseal) vascular grafts were compared, the grafts being coated with three different matrices: collagen IV, fibronectin and preclot matrix. In addition, untreated ePTFE and Gelseal were examined. The graft segments, coated with these matrices, were incubated with radiolabelled adult human endothelial cells for 30, 60 and 90 min. Endothelial cell adherence was calculated from the ratio of radioactive counts in the grafts to counts in grafts plus supernatants. Endothelial cell attachment to untreated grafts was poor, but a suitable matrix significantly improved adherence. All three matrices tested gave good results, although preclot was best; 30-60 min incubation was sufficient for optimum cell attachment. Cell adherence to both Dacron and ePTFE was significantly better than to Gelseal. The type of prosthetic polymer and the substrate protein coating used to promote endothelial cell adherence are two important factors which may determine the ultimate success of endothelial seeding in the operating room.
Vohra
R
R
Department of Vascular Surgery and Medical Biophysics, Manchester Royal Infirmary, UK.
Thomson
G J
GJ
Carr
H M
HM
Sharma
H
H
Walker
M G
MG
D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D002478:Cells, Cultured; D003094:Collagen; D004730:Endothelium, Vascular / Q000166:cytology*; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D005780:Gelatin; D066298:In Vitro Techniques; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D011093:Polyethylene Terephthalates; D011138:Polytetrafluoroethylene; D014656:Vascular Surgical Procedures
D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D011093:Polyethylene Terephthalates; D005780:Gelatin; D011138:Polytetrafluoroethylene; D003094:Collagen