Human erythrocyte multicatalytic proteinase: activation and binding to sulfated galacto- and lactosylceramides.
Chymotrypsin-like activity of multicatalytic proteinase (MCP) purified from human erythrocytes was selectively activated 2.5--3.5-fold by sulfated glycolipids such as galactosylceramide sulfate (SM4) and lactosylceramide sulfate (SM3) but not by other glycolipids including galactosylceramide (GalCer), lactosylceramide (LacCer), GD1a, GM1 and GM3. Heparin also selectively activated trypsin-like activity 2.5-fold, while other mucopolysaccharides did not. This proteinase molecule bound specifically and with high affinity to both SM4 and SM3, but not to GalCer, LacCer and GM3. The binding of SM4 and SM3 to the enzyme molecule was also confirmed by thin layer chromatography.
Ohkubo
I
I
Department of Biochemistry, Nagoya City University Medical School, Japan.
Gasa
S
S
Namikawa
C
C
Makita
A
A
Sasaki
M
M
D000595:Amino Acid Sequence; D015703:Antigens, CD; D002240:Carbohydrate Sequence; D003546:Cysteine Endopeptidases / Q000097:blood* / Q000302:isolation & purification; D004789:Enzyme Activation; D004912:Erythrocytes / Q000201:enzymology*; D005699:Galactosylceramides / Q000378:metabolism / Q000494:pharmacology*; D005732:Gangliosides / Q000494:pharmacology; D006017:Glycolipids / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000494:pharmacology; D006028:Glycosphingolipids / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D007790:Lactosylceramides; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000097:blood* / Q000302:isolation & purification; D046988:Proteasome Endopeptidase Complex; D011485:Protein Binding; D013379:Substrate Specificity
D015703:Antigens, CD; D005699:Galactosylceramides; D005732:Gangliosides; D006017:Glycolipids; D006025:Glycosaminoglycans; D006028:Glycosphingolipids; D007790:Lactosylceramides; D009097:Multienzyme Complexes; C009744:CDw17 antigen; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex