Preparation of Japanese encephalitis virus nonstructural protein NS1 obtained from culture fluid of JEV-infected Vero cells.
The Japanese encephalitis virus (JEV) nonstructural protein NS1 was released efficiently into culture fluid of JEV-infected Vero cells. The JEV NS1 protein in the infected culture fluid was found almost as a high-molecular-weight form, probably a dimer form of NS1, and was converted to a monomer by boiling. Large amounts of NS1 protein were accumulated in the infected culture fluid. The NS1 protein, separated from JE virions by centrifugation through sucrose layer, could be obtained in large quantities.
Lee
T
T
Department of Virology, Kitasato Institute, Tokyo, Japan.
Watanabe
K
K
Aizawa
C
C
Nomoto
A
A
Hashimoto
H
H
D000818:Animals; D015153:Blotting, Western; D002213:Capsid / Q000096:biosynthesis* / Q000302:isolation & purification; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D003588:Cytopathogenic Effect, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D004664:Encephalitis Virus, Japanese / Q000378:metabolism*; D006358:Hot Temperature; D008970:Molecular Weight; D014709:Vero Cells; D014758:Viral Core Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D017361:Viral Nonstructural Proteins; D014771:Virion / Q000302:isolation & purification
D003470:Culture Media; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins