title: Lymphocyte subsets in whole blood and isolated mononuclear leucocytes of healthy infants and children.
abstract: The determination of lymphocyte subsets utilizing monoclonal antibodies and flow cytometry has become essential in the evaluation of immunological status. Using a standardized method it was found that in healthy children the percentage of CD 8+ (Leu 2+) positive cells increases significantly (P less than 0.01) during infancy, whereas the percentage of CD 4+ (Leu 3+) positive cells decreases with age (P less than 0.01). The percentage of CD 3+ (Leu 4+) cells remains constant. The ratio of CD 4/CD 8 positive cells is significantly (P less than 0.001) higher in infants than in older children. Other subpopulations (HLA DR+, Leu 7) were found to be constant in all age groups. For the comparison of data on lymphocyte subsets obtained by flow cytometry a standardized test procedure is important.
authors.affiliation: Department for Paediatric Pneumology and Immunology, Universitäts-Kinderklinik FU Berlin, Federal Republic of Germany.
authors.forename: N
authors.initials: N
authors.lastname: Remy
authors.forename: M
authors.initials: M
authors.lastname: Oberreit
authors.forename: G
authors.initials: G
authors.lastname: Thoenes
authors.forename: U
authors.initials: U
authors.lastname: Wahn
mesh_terms: D000293:Adolescent; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007223:Infant; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D016131:Lymphocyte Subsets / Q000166:cytology*; D008297:Male; D015203:Reproducibility of Results; D006377:T-Lymphocytes, Helper-Inducer; D050378:T-Lymphocytes, Regulatory
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD