Depletion of intracellular free magnesium in rat hearts during acute alcohol perfusion: a 31P nuclear magnetic resonance study.
The effect of acute ethanol perfusion on the intracellular free magnesium level, intracellular pH, and high energy phosphate levels of isolated adult rat hearts (n = 13) were studied using 31P nuclear magnetic resonance (NMR) spectroscopy. Perfusion with 1% ethanol solution resulted in a 43% decrease in the intracellular free magnesium level in Langendorff perfused rat hearts while intracellular pH was not significantly altered. In most hearts ethanol perfusion also resulted in increased intracellular inorganic phosphate and reduced phosphocreatine and ATP levels, corresponding to a significant reduction in phosphorylation potential. The implications of these results in the pathogenesis of alcohol-induced hypertension are discussed in light of our previous studies on spontaneously hypertensive and normotensive rats which demonstrated a correlation between cardiac free magnesium levels and blood pressure.
Jelicks
L A
LA
Department of Physiology, Albert Einstein College of Medicine, Yeshiva University, Bronx, N.Y.
Gupta
R K
RK
D000818:Animals; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D006321:Heart / Q000187:drug effects*; D066298:In Vitro Techniques; D008274:Magnesium / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010477:Perfusion; D010758:Phosphorus; D051381:Rats; D017207:Rats, Sprague-Dawley
D010758:Phosphorus; D000431:Ethanol; D008274:Magnesium