[Hemolytic activity of venoms from snakes of the genera Bothrop, Lachesis, Crotalus, and Micrurus (Serpentes: Viperidae and Elapidae].
Hemolytic activity of eight Peruvian snake venoms from the families Viperidae and Elapidae (Bothrops atrox, B. pictus, B. hyoprorus, B. bilineatus, B. neuwedii, Lachesis m. muta, Crotalus d. terrificus, Micrurus tschudi), and three Brazilian viperids (B. jararacussu, B. alternatus and C. d. collilineatus) is described. None of the venoms caused direct lysis on washed human erythrocytes. However, all of them caused indirect hemolysis provided that the incubation medium contains an exogenous source of lecithin. Venom of Micrurus tschudi was the most hemolytic (HD50 2.8 ug/ml) while that of B. bilineatus was the least (HD50 681.3 ug/ml). Only six of eleven venoms showed parallel curves of hemolytic activity, and the HD50 varied from 198 to 681 ug/ml and the following decreasing order of hemolytic activity was obtained: L. muta, C. d. terrificus, C. d. collilineatus, B. hyoprorus, B. bilineatus, B. alternatus.
Martínez Cadillo
E
E
Centro de Investigación en Salud Hugo Lumbreras Cruz, Instituto Nacional de Salud, Lima, Perú.
Bonilla Ferreyra
C
C
Zvealeta
A
A
D000818:Animals; D004179:Cobra Cardiotoxin Proteins / Q000494:pharmacology; D004546:Elapid Venoms / Q000494:pharmacology*; D006461:Hemolysis; D006801:Humans; D010741:Phospholipases A / Q000494:pharmacology; D012044:Regression Analysis; D014757:Viper Venoms / Q000494:pharmacology*
D004179:Cobra Cardiotoxin Proteins; D004546:Elapid Venoms; D014757:Viper Venoms; D010741:Phospholipases A
Actividad hemolítica de venenos de serpientes de los géneros Bothrops, Lachesis, Crotalus y Micrurus (Serpentes: Viperidae y Elapidae).