Moloney murine leukemia virus tolerance in anti-CD4 monoclonal antibody-treated adult mice.
Adult mice injected with Moloney murine leukemia virus (M-MuLV) 1 day after a short term treatment with anti-CD4 mAb developed T cell lymphomas, or sarcomas when rechallenged with Moloney murine sarcoma virus (M-MSV). Neoplastic development was correlated with virus spread, as thymic and peripheral T and B lymphocytes promptly expressed M-MuLV-induced Ag after virus introduction; no virus-specific CTL generation was detected in MLTC. This failure, which was selective for M-MuLV-induced Ag, persisted throughout the life span of the mice, and was not sustained by suppressor cell activity. The frequencies of splenic virus-specific CTL precursor varied in relation to time after virus injection; in the first postinjection month, frequencies were similar to those in nonimmune control mice, while at 4 mo post-virus exposure, they showed a striking reduction. These findings indicate that a transient functional impairment of CD4+ cells at the time of retrovirus injection provided the appropriate milieu for tolerance induction in both the peripheral mature and intrathymic T cell compartments.
Biasi
G
G
Institute of Oncology, CIRC, University of Padova, Italy.
Facchinetti
A
A
Panozzo
M
M
Zanovello
P
P
Chieco-Bianchi
L
L
Collavo
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis; D015704:CD4 Antigens / Q000276:immunology*; D007108:Immune Tolerance; D007942:Leukemia, Experimental / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008979:Moloney murine leukemia virus / Q000276:immunology*; D008980:Moloney murine sarcoma virus / Q000276:immunology; D051381:Rats; D012513:Sarcoma, Experimental / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology; D050378:T-Lymphocytes, Regulatory / Q000502:physiology
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D015704:CD4 Antigens