A procedure for rapid isolation of cloned cDNA inserts using the polymerase chain reaction technique.
A reproducible and rapid procedure for isolation of cloned cDNA insert from a lambda gt11 cDNA library is described. The procedure relies on the polymerase chain reaction method using forward and reverse primers for lambda gt11, followed by isolation of the cloned cDNA insert by a rapid technique. The procedure should also be applicable to isolation of cDNA inserts cloned in other vectors such as lambda gt10.
Goueli
S A
SA
Department of Laboratory Medicine and Pathology, University of Minnesota, School of Medicine, Minneapolis.
Ahmed
K
K
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D015342:DNA Probes; D015246:Deoxyribonuclease EcoRI; D004331:Drosophila melanogaster / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000235:genetics; D017346:Protein Serine-Threonine Kinases
D015342:DNA Probes; D004247:DNA; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D015246:Deoxyribonuclease EcoRI