title: Neurospora crassa clock-controlled genes are regulated at the level of transcription.
abstract: Although an extensive number of biological processes are under the daily control of the circadian biological clock, little is known about how the clock maintains its regulatory networks within a cell. An important aspect of this temporal control is the daily control of gene expression. Previously we identified two morning-specific genes that are regulated by the clock through daily control of gene expression (J. Loros, S. Denome, and J.C. Dunlap, Science 243:385-388, 1989). We have now introduced a method for transcriptional analysis in Neurospora crassa and used this nuclear run-on procedure to show that regulation of mRNA abundance for these two morning-specific genes occurs at the level of transcription. This transcriptional regulation by the circadian clock provides a basis for isolating circadian rhythm mutants.
authors.affiliation: Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03756.
authors.forename: J J
authors.initials: JJ
authors.lastname: Loros
authors.forename: J C
authors.initials: JC
authors.lastname: Dunlap
mesh_terms: D000546:Amanitins / Q000494:pharmacology; D002467:Cell Nucleus / Q000502:physiology; D002940:Circadian Rhythm; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D009492:Neurospora crassa / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D000546:Amanitins; D012331:RNA, Fungal; D012333:RNA, Messenger