The renal excretory activity of atrial natriuretic factor is independent of renal prostaglandins in humans.
Since renal prostaglandins may contribute to natriuresis induced by endogenous atrial natriuretic factor (ANF), acute volume expansion (AVL), a known stimulus of ANF and prostaglandins, was induced in 8 healthy women in order to test whether the consequent sodium and water diuresis is altered by prostaglandin inhibition. AVL (i.v. infusion of a 2 liter 5% glucose solution in 1 h) was infused after placebo and after inhibition of prostaglandins with diclofenac (200 mg/day orally for 4 days), in a double blind randomized cross-over fashion. Urinary eicosanoids (PGE2, PGF2 alpha, 6-ketoPGF1 alpha, TXB2--RIA), plasma ANF (RIA) and urinary electrolytes were determined before, during and after AVL under both placebo and diclofenac regimes. During placebo, AVL induced sustained increases in plasma ANF (174% at peak, p less than 0.001 ANOVA), excretion of the four eicosanoids (149%-1172%, p less than 0.005-0.001), urinary volume (UV, 815%, p less than 0.001), natriuresis (UNa, 98%, p less than 0.005) and in kaliuresis (UK, 90%, p less than 0.001). Cyclooxygenase inhibition resulted in a reduction of over 70% in both baseline values and AVL-induced increase of eicosanoids. It did not alter either baseline levels or AVL-stimulated ANF, UV, UNa and UK in relation to placebo. The present results suggest that the diuretic and natriuretic activity of ANF is not mediated by renal PGs in humans.
Castellani
S
S
Clinica Medica I, University of Florence, Italy.
Del Rosso
A
A
Chen
J L
JL
Camaiti
A
A
Carnovali
M
M
Masotti
G
G
D000328:Adult; D009320:Atrial Natriuretic Factor / Q000097:blood*; D004231:Diuresis; D004573:Electrolytes; D005260:Female; D006801:Humans; D007668:Kidney / Q000201:enzymology / Q000378:metabolism*; D008875:Middle Aged; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011453:Prostaglandins / Q000652:urine*; D011863:Radioimmunoassay; D012964:Sodium / Q000652:urine
D004573:Electrolytes; D011453:Prostaglandins; D009320:Atrial Natriuretic Factor; D012964:Sodium; D011451:Prostaglandin-Endoperoxide Synthases