title: Molecular organisation of the malate synthase genes of Aspergillus nidulans and Neurospora crassa.
abstract: The sequencing and comparison of the genes encoding the glyoxylate bypass enzyme malate synthase of Aspergillus nidulans (acuE) and Neurospora crassa (acu-9) are presented. The predicted amino acid sequences of the A. nidulans and N. crassa enzymes are 538 and 542 residues respectively and the proteins are 87% homologous. In fungi, the malate synthase proteins are located in glyoxysomes and the deduced acuE and acu-9 proteins both contain a C-terminal S-K-L sequence, which has been implicated in transport into peroxisomes. The acuE coding region is interrupted by four introns and the acu-9 coding region is interrupted by one intron which occurs at the same position as the C-terminal acuE intron. The 5' non-coding regions of the two genes were examined for short homologous sequences that may represent the binding sites for regulatory proteins. Pyrimidine-rich sequences with weak homology to the amdI9 sequence, which has been implicated in facB-mediated acetate regulation of the amdS gene, were found but their functional significance remains to be determined.
authors.affiliation: Department of Genetics, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: R A
authors.initials: RA
authors.lastname: Sandeman
authors.forename: M J
authors.initials: MJ
authors.lastname: Hynes
authors.forename: J R
authors.initials: JR
authors.lastname: Fincham
authors.forename: I F
authors.initials: IF
authors.lastname: Connerton
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D005800:Genes, Fungal; D008292:Malate Synthase / Q000235:genetics*; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000201:enzymology* / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D008292:Malate Synthase