title: Immunogold localisation of ubiquitin-protein conjugates in primary (azurophilic) granules of polymorphonuclear neutrophils.
abstract: Ubiquitin-protein conjugates are found in the primary (azurophilic) lysosome-related granules but not in the secondary (specific) granules in mature polymorphonuclear neutrophils prepared from bone marrow. This is the first reported demonstration of ubiquitin-protein conjugates in lysosome-related membrane-bound vesicles in granulocytes and complements our previous findings of ubiquitinated proteins in lysosomes of fibroblasts. The significance of the selective presence of conjugates in only one of the two main types of neutrophil granules remains to be elucidated but may relate to the presence of the complement of acid hydrolases, including proteases, in the azurophilic granules compared to the specific granules. Ubiquitin-protein conjugates may enter the primary granules during neutrophil maturation by an autophagic process or by a heterophagic process during the fusion of phagosomes with primary granules. Alternatively protein ubiquitination may be involved in granule biogenesis.
authors.affiliation: Department of General Zoology, Eötvös University, Budapest, Hungary.
authors.forename: L
authors.initials: L
authors.lastname: László
authors.forename: F J
authors.initials: FJ
authors.lastname: Doherty
authors.forename: A
authors.initials: A
authors.lastname: Watson
authors.forename: T
authors.initials: T
authors.lastname: Self
authors.forename: M
authors.initials: M
authors.lastname: Landon
authors.forename: J
authors.initials: J
authors.lastname: Lowe
authors.forename: R J
authors.initials: RJ
authors.lastname: Mayer
mesh_terms: D000818:Animals; D002451:Cell Compartmentation; D003594:Cytoplasmic Granules / Q000378:metabolism*; D007150:Immunohistochemistry; D008247:Lysosomes / Q000378:metabolism; D008854:Microscopy, Electron; D009504:Neutrophils / Q000648:ultrastructure*; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000378:metabolism; D014452:Ubiquitins / Q000378:metabolism*
chemical_list: D011956:Receptors, Cell Surface; D014452:Ubiquitins