Plasmin inhibitory effect of rat tissues: comparison with antiplasmin activity of plasma.
Plasmin inhibition by rat tissue extracts was compared with rat plasma plasmin inhibition. Nine different tissues were extracted with an acid acetate-buffer (lung, heart, brain, adrenal, aorta, skeletal muscle, spleen and liver) or with KSCN-buffer (kidney), and antiplasmin activity was evaluated by a spectrophotometric method. All tissue extracts inhibited plasmin dose-dependently; the amount of plasmin inhibited by a fixed amount of extract depended upon the tissue. In rat plasma, antiplasmin activity was acid-labile, but acid treatment did not affect the plasmin inhibitory effect of tissues extracts.
Padró
T
T
Gaubius Laboratory IVVO-TNO, Leiden, The Netherlands.
van den Hoogen
C M
CM
Emeis
J J
JJ
D000818:Animals; D005341:Fibrinolysin / Q000037:antagonists & inhibitors*; D006863:Hydrogen-Ion Concentration; D051381:Rats; D011919:Rats, Inbred Strains; D014020:Tissue Extracts / Q000502:physiology*; D000979:alpha-2-Antiplasmin / Q000502:physiology*
D014020:Tissue Extracts; D000979:alpha-2-Antiplasmin; D005341:Fibrinolysin