Anti-Sm sera are misdetected by counterimmunoelectrophoresis. A critical analysis of CIE and western blotting on the detection of human antinuclear antibodies.
The ability of CIE and Western blotting to detect antibodies against the Sm, RNP and SS/B antigens was tested in 84 sera from SLE patients. CIE and Western blotting gave identical results for anti-RNP and anti-SS/B; positivity was detected in the same sera regardless of the technique used and no new positive sera were found by Western blotting among those classified by CIE as not showing immunological relationship to the anti-RNP and anti-SS/B references. CIE detected only half of the anti-Sm that were positive by Western blotting. The inability of CIE to detect sera positive for anti-Sm could not be ascribed to specific features of the sera, such as low titer or the lack of antibodies to the Sm antigens B/B' and/or D. The identity of a number of these new anti-Sm sera was confirmed by the utilization of an in vitro translation test, where the 'cell-free' translation products were immunoprecipitated with the new positive anti-Sm sera.
Navarro
E
E
Centre d'Investigació i Desenvolupament de Barcelona (CSIC), Spain.
Palau
J
J
D000974:Antibodies, Antinuclear / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology*; D001327:Autoimmune Diseases / Q000276:immunology*; D015153:Blotting, Western; D003378:Counterimmunoelectrophoresis; D005188:False Negative Reactions; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D012261:Ribonucleoproteins / Q000276:immunology; D017411:Ribonucleoproteins, Small Nuclear; D055517:snRNP Core Proteins; D000097585:SS-B Antigen
D000974:Antibodies, Antinuclear; D001324:Autoantigens; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D055517:snRNP Core Proteins; D000097585:SS-B Antigen