A stable isotope dilution mass spectrometric assay for the major urinary metabolite of PGD2.
1. A sensitive and specific negative ion chemical ionization mass spectrometric assay for the major urinary metabolite of PGD2 has been developed employing a chemically synthesized [18(0)4]-labelled internal standard. 2. The finding that increased urinary excretion of this metabolite occurs in a number of clinical situations suggests that the assay may prove to be a valuable tool to explore the role of PGD2 in the pathophysiology of human disease.
Morrow
J D
JD
Department of Pharmacology, Vanderbilt University, Nashville, TN 37232-6602.
Prakash
C
C
Duckworth
T A
TA
Zackert
W E
WE
Blair
I A
IA
Oates
J A
JA
Roberts
L J
LJ
D001249:Asthma / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D006632:Histamine / Q000378:metabolism; D006801:Humans; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism; D006937:Hypercholesterolemia / Q000188:drug therapy / Q000652:urine; D007201:Indicator Dilution Techniques; D013058:Mass Spectrometry / Q000379:methods*; D008415:Mastocytosis / Q000652:urine; D009525:Niacin / Q000494:pharmacology / Q000627:therapeutic use; D015230:Prostaglandin D2 / Q000378:metabolism*; D011457:Prostaglandins D / Q000032:analysis* / Q000096:biosynthesis
D011457:Prostaglandins D; D009525:Niacin; C035860:9-hydroxy-11,15-dioxo-2,3,18,19-tetranorprost-5-ene-1,20-dioic acid; D006632:Histamine; D006911:Hydroxyprostaglandin Dehydrogenases; C031051:prostaglandin-F synthase; D015230:Prostaglandin D2