Oligonucleotide primers for DNA amplification of the early regions 1, 6, and 7 from human papillomavirus types 6, 11, 16, 18, 31, and 33.
Human papillomavirus (HPV) type-specific sequences required for polymerase chain reaction (PCR) mediated amplification of HPV DNA sequences are presented. One primer pair within the E1 open reading frame (ORF) was shared by HPV 6, HPV 11, HPV 16, and HPV 31, whereas the other primer pair within the E1 ORF was specific for HPV 16. Eight primer pairs from the E6 and E7 ORFs specifically detected HPV 6, HPV 16, HPV 18, and HPV 33 sequences. This system has been used for detection of HPV DNA in biopsies, cytological smears and sections of formalin-fixed tissues.
Evander
M
M
Department of Virology, University of Umeå, Sweden.
Bodén
E
E
Bjersing
L
L
Rylander
E
E
Wadell
G
G
D001483:Base Sequence; D015139:Blotting, Southern; D004279:DNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D009841:Oligonucleotides / Q000235:genetics; D016366:Open Reading Frames; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D014412:Tumor Virus Infections / Q000382:microbiology; D002578:Uterine Cervical Dysplasia / Q000382:microbiology
D004279:DNA, Viral; D009841:Oligonucleotides