[A comparative study of the functions of recBCD-nuclease from Escherichia coli and "recombinase", encoded by plasmid R1drd-19].
The RecBCD nuclease of Escherichia coli and "recombinase" determined by R1drd-19 plasmid (the latter is able to replace at least partially the indicated cellular enzyme) were shown to differ from each other in some essential features. The product encoded by the plasmid as distinct from RecBCD nuclease practically is not sensitive to inhibition by GamS protein of the lambda phage. Earlier, it was found that the presence of R1drd-19 plasmid in the recBC cells restores the level of the total ATP-dependent exonuclease activity because of appearance in such cells of a new exonuclease activity also ATP-dependent. The exonuclease activity determined by R1drd-19 plasmid was found to differ from the corresponding activity of the RecBCD enzyme. The plasmid enzyme was able to prevent reproduction of T4g2- mutant on recBC cells. The ability of the plasmid "recombinase" to some stimulation of intrachromosomal recombination in recA mutant witness to incomplete RecA-dependence of its function. No significant homology was registered between Escherichia coli DNA fragment containing the recB, recC, recD genes and the EcoRI-C-fragment of R1drd-19 carrying the sequences responsible for recombination and repair functions of the plasmid.
Terent'ev
M A
MA
Ovadis
M I
MI
Denisova
T S
TS
Chernin
L S
LS
D010582:Bacteriophage lambda / Q000378:metabolism; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004264:DNA Topoisomerases, Type I / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics*; D005798:Genes, Bacterial; D019426:Integrases; D010957:Plasmids; D045522:Recombinases; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000378:metabolism
D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D045522:Recombinases; D014764:Viral Proteins; C065410:gam protein, Coliphage; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C115006:integron integrase IntI1; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli; D004264:DNA Topoisomerases, Type I
Sravnitel'noe issledovanie funktsiĭ recBCD-nukleazy Escherichia coli i "recombinazy", kodiruemoĭ plazmidoĭ R1drd-19.