Interactions between interleukin-1 and basic fibroblast growth factor on articular chondrocytes. Effects on cell growth, prostanoid production, and receptor modulation.
The interactions of interleukin-1 beta (IL-1 beta) and basic fibroblast growth factor (bFGF) with monolayer cultures of rabbit articular chondrocytes were examined. Both agonists resulted in a synergistic increase in prostanoid production, to levels higher than the maximal level for either protein alone. The synergy was time- and dose-dependent, was augmented in serum-free medium, and was blocked by indomethacin or a polyclonal antibody to IL-1 beta. The proteins had opposite effects on cell growth, and IL-1 beta completely blocked the mitogenic effect of bFGF. Pretreatment of cells with IL-1 beta induced a down-regulation in the number of the bFGF high-affinity receptors. Pretreatment of cells with bFGF increased the number of IL-1 receptors, which was dependent on messenger RNA synthesis.
Chin
J E
JE
Hypersensitivity Diseases Research, Upjohn Company, Kalamazoo, MI 49001.
Hatfield
C A
CA
Krzesicki
R F
RF
Herblin
W F
WF
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis*; D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology* / Q000378:metabolism; D002455:Cell Division; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis*; D004357:Drug Synergism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology*; D007375:Interleukin-1 / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017468:Receptors, Fibroblast Growth Factor; D011971:Receptors, Immunologic / Q000378:metabolism*; D017472:Receptors, Interleukin-1
D007375:Interleukin-1; D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D016222:Fibroblast Growth Factor 2; D015121:6-Ketoprostaglandin F1 alpha; D015232:Dinoprostone