High-level expression of porcine growth hormone gene in Escherichia coli by using temperature-inducible vectors.
Temperature-inducible expression vectors, containing the porcine growth hormone gene, were constructed and used to transform E. coli N4830. After temperature shift induction, high-level expression was achieved. The expressed protein proved to be an authentic product of the porcine growth hormone gene by SDS-PAGE and Western blot analysis. The product accounts for approximately 30% of the total bacterial cellular proteins, as estimated by Shimadzu CS-910 scanning of the SDS-PAGE gel stained by Coomassie brilliant blue R250.
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Laboratory of Animal Biochemistry, Beijing Agricultural University, China.
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D000818:Animals; D015153:Blotting, Western; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005822:Genetic Vectors; D013006:Growth Hormone / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis*; D013552:Swine; D013696:Temperature; D014158:Transcription, Genetic
D011994:Recombinant Proteins; D013006:Growth Hormone