The acyl-carrier protein in Neurospora crassa mitochondria is a subunit of NADH:ubiquinone reductase (complex I).
We determined the primary structure of a 9.6-kDa subunit of the respiratory chain NADH:ubiquinone reductase (complex I) from Neurospora crassa mitochondria and found a close relationship between this subunit and the bacterial or chloroplast acyl-carrier protein. The degree of sequence identity amounts to 80% in a region of 19 residues around the serine to which the phosphopantetheine is bound. The N-terminal presequence of the subunit has the characteristic features of a mitochondrial import sequence. We cultivated the auxotroph pan-2 mutant of N. crassa in the presence of [14C]pantothenate and recovered all radioactivity incorporated into mitochondrial protein in the 9.6-kDa subunit of complex I. We cultivated N. crassa in the presence of chloramphenicol to accumulate the nuclear-encoded peripheral arm of complex I. This pre-assembled arm also contains the 9.6-kDa subunit. These results demonstrate that an acyl-carrier protein with pantothenate as prosthetic group is a constituent part of complex I in N. crassa.
Sackmann
U
U
Institut für Biochemie, Universität Düsseldorf, Federal Republic of Germany.
Zensen
R
R
Röhlen
D
D
Jahnke
U
U
Weiss
H
H
D000213:Acyl Carrier Protein / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal; D004591:Electrophoresis, Polyacrylamide Gel; D008715:Methionine / Q000737:chemistry; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D016660:NAD(P)H Dehydrogenase (Quinone); D009492:Neurospora crassa / Q000201:enzymology*; D010205:Pantothenic Acid / Q000737:chemistry; D011808:Quinone Reductases / Q000378:metabolism*; D016415:Sequence Alignment
D000213:Acyl Carrier Protein; D004271:DNA, Fungal; D010205:Pantothenic Acid; D008715:Methionine; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases