Cloning, sequencing and tissue distribution of a candidate G protein-coupled receptor from rat pituitary gland.
A new member of the family of G protein-coupled receptors has been isolated from a rat pituitary cDNA library by the polymerase chain reaction (PCR) using degenerate oligonucleotide primers. The corresponding protein sequence shows seven transmembrane domains and contains conserved regions of homology characteristic of the G protein-coupled class of receptors. The novel receptor mRNA is expressed in the brain, pituitary gland and testis, and has been localized by in situ hybridization in discrete regions of the brain. Expression of the receptor mRNA in Xenopus oocytes and in transfected mammalian cells has not yet permitted identification of the corresponding ligand for this receptor.
Eidne
K A
KA
MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburg, UK.
Zabavnik
J
J
Peters
T
T
Yoshida
S
S
Anderson
L
L
Taylor
P L
PL
D000595:Amino Acid Sequence; D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010902:Pituitary Gland / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D051381:Rats; D016415:Sequence Alignment; D014018:Tissue Distribution; D014158:Transcription, Genetic
D015345:Oligonucleotide Probes; D004247:DNA; D019204:GTP-Binding Proteins