Effect of aging and diet on proton NMR spectra of rat urine.
500 MHz 1H NMR studies of rat urine are used to assess metabolic changes resulting from aging (0.5-20 months) and changes in diet (casein versus chow). Aging rats fed chow diets decrease their excretion of citrate and 2-oxoglutarate, while the output of taurine and creatinine increases. Only young rats (1 month or less postweaning) excrete significant amounts of betaine and trimethylamine-N-oxide. Rats fed casein diets for 1 month postweaning do not excrete 2-oxoglutarate and excrete lower levels of hippurate, succinate, and citrate compared to rats fed chow diets. They also excrete N-methylnicotinamide. These high resolution proton NMR studies provide metabolic profiles which are not readily available by other techniques.
Bell
J D
JD
Department of Chemistry, Birkbeck College, University of London, Gordon House and Christopher Ingold Laboratory, London, England.
Sadler
P J
PJ
Morris
V C
VC
Levander
O A
OA
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002364:Caseins / Q000008:administration & dosage; D002951:Citrates / Q000652:urine; D019343:Citric Acid; D003404:Creatinine / Q000652:urine; D004032:Diet; D006023:Glycoproteins / Q000652:urine; D006626:Hippurates / Q000652:urine; D006859:Hydrogen; D007656:Ketoglutaric Acids / Q000652:urine; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009536:Niacinamide / Q000031:analogs & derivatives / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains; D013395:Sucrose / Q000008:administration & dosage; D013654:Taurine / Q000652:urine; D014556:Urine / Q000737:chemistry*
D002364:Caseins; D002951:Citrates; D006023:Glycoproteins; D006626:Hippurates; D007656:Ketoglutaric Acids; D013654:Taurine; D009536:Niacinamide; D019343:Citric Acid; D013395:Sucrose; D006859:Hydrogen; D003404:Creatinine; C030514:hippuric acid; C008472:N-methylnicotinamide