title: Correlations between follicular fluid steroid analysis and maturity and cytogenetic analysis of human oocytes that remained unfertilized after in vitro fertilization.
abstract: OBJECTIVE
  Is there any correlation between follicular fluid (FF) steroid levels and the occurrence of cytogenetic abnormalities in unfertilized human oocytes?
  
  
  METHODS
  Cytogenetic analysis was carried out on 397 oocytes, and the steroid content of 104 corresponding FF was analyzed using high-pressure liquid chromatography. Ovarian stimulation was performed by clomiphene citrate and human menopausal gonadotropin (hMG) or by hMG combined with a gonadotropin-releasing hormone agonist (GnRH-a) pretreatment.
  
  
  RESULTS
  Oocyte maturity was correlated with an increasing FF progestin content and a significant decrease of androstenedione (A) levels. Chromosomal analysis revealed 84 of all oocytes to be abnormal (polyploid or aneuploid and/or prematurely condensed chromosomes present). In this group, A levels and A to estradiol ratios were significantly higher. Although progestin levels were higher in GnRH-a/hMG cycles, the incidence of oocyte normality was not different between the two stimulation schemes. More abnormal oocytes were found in patients with good sperm morphology.
  
  
  CONCLUSIONS
  Oocyte abnormality correlates with higher A levels in the corresponding FF. Oocyte fertilization is also determined by intrinsic oocytic factors other than maturity.
authors.affiliation: Department of Gynecology and Obstetrics, State University of Ghent, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: De Sutter
authors.forename: M
authors.initials: M
authors.lastname: Dhont
authors.forename: E
authors.initials: E
authors.lastname: Vanluchene
authors.forename: D
authors.initials: D
authors.lastname: Vandekerckhove
mesh_terms: D019326:17-alpha-Hydroxyprogesterone; D004092:20-alpha-Dihydroprogesterone / Q000378:metabolism; D000735:Androstenedione / Q000378:metabolism; D002064:Buserelin / Q000031:analogs & derivatives / Q000627:therapeutic use; D002875:Chromosomes / Q000648:ultrastructure*; D002996:Clomiphene / Q000627:therapeutic use; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000378:metabolism*; D017273:Goserelin; D006801:Humans; D006908:Hydroxyprogesterones / Q000378:metabolism; D007621:Karyotyping; D008297:Male; D008596:Menotropins / Q000627:therapeutic use; D009865:Oocytes / Q000502:physiology* / Q000648:ultrastructure; D011003:Ploidies; D011374:Progesterone / Q000378:metabolism; D011372:Progestins / Q000378:metabolism; D013256:Steroids / Q000378:metabolism*
chemical_list: D006908:Hydroxyprogesterones; D011372:Progestins; D013256:Steroids; D017273:Goserelin; C018975:16-hydroxyprogesterone; D004092:20-alpha-Dihydroprogesterone; D002996:Clomiphene; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D008596:Menotropins; D019326:17-alpha-Hydroxyprogesterone; D002064:Buserelin