title: Sequence analysis of a glycoprotein D gene homolog within the unique short segment of the EHV-1 genome.
abstract: DNA sequence analysis of one-third of the unique short (Us) segment of the equine herpesvirus type 1 (EHV-1) genome revealed an open reading frame (ORF) whose translated sequence exhibits significant homology to glycoprotein D of herpes simplex virus (HSV) types 1 and 2 and to pseudorabies virus (PRV) glycoprotein 50, the gD equivalent. The ORF of the EHV-1 gD homolog lies within the pSZ-4 BamHI/KpnI fragment (map units 0.865 to 0.872 and 0.869 to 0.884) and is capable of encoding a polypeptide of 385 amino acids (43,206 molecular weight). Analysis of the nucleotide sequence revealed a complete transcriptional unit including CAAT and TATA elements and signals for polyadenylation. The predicted protein exhibits features typical of a transmembrane protein: a hydrophobic N-terminal signal sequence followed by a probable cleavage site, four potential N-linked glycosylation sites, and a hydrophobic membrane-spanning domain near the carboxyl terminus followed by a charged membrane anchor sequence.
authors.affiliation: Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport 71130.
authors.forename: C C
authors.initials: CC
authors.lastname: Flowers
authors.forename: E M
authors.initials: EM
authors.lastname: Eastman
authors.forename: D J
authors.initials: DJ
authors.lastname: O'Callaghan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral; D004861:Herpesvirus 1, Equid / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins; C488988:glycoprotein D, Equid herpesvirus 1; C040570:glycoprotein D-herpes simplex virus type 2; C042966:pseudorabies virus glycoproteins