Protection against lethal Sendai virus infection by in vivo priming of virus-specific cytotoxic T lymphocytes with a free synthetic peptide.
The only peptide of Sendai virus that is recognized by cytotoxic T lymphocytes (CTL) in B6 mice was found with (i) the use of recombinant vaccinia virus constructs containing separate genes of Sendai virus and (ii) a set of overlapping peptides completely spanning the identified nucleoprotein (NP) gene product. This immunodominant NP peptide is recognized by Sendai virus-specific CTL that are known to have therapeutic effects in vivo. By subcutaneous immunization, this peptide induced Sendai virus and NP peptide-specific CTL memory responses in vivo. Most importantly, mice that had been immunized with this peptide were protected against a lethal virus dose, indicating that viral peptides can be used as antiviral T-cell vaccines. The induction of T-cell memory by free peptide immunization potentially has wide applicability in biology and medicine, including protection against infectious disease.
Kast
W M
WM
Division of Immunology, The Netherlands Cancer Institute, Amsterdam.
Roux
L
L
Curren
J
J
Blom
H J
HJ
Voordouw
A C
AC
Meloen
R H
RH
Kolakofsky
D
D
Melief
C J
CJ
D000818:Animals; D002478:Cells, Cultured; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D051379:Mice; D008815:Mice, Inbred Strains; D010222:Parainfluenza Virus 1, Human / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D018184:Paramyxoviridae Infections / Q000276:immunology* / Q000517:prevention & control; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage* / Q000494:pharmacology; D014616:Vaccinia virus / Q000235:genetics; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000494:pharmacology
D014614:Vaccines, Synthetic; D014765:Viral Vaccines