title: Transformation and establishment of fragile X cell lines from amniocytes.
abstract: Fragile X [fra(X)] cell lines have been established from an expressing 46,XX amniocyte culture. The amniocyte cells were transformed by the addition of an origin defective pSV40 vector. Fra(X) expression was observed at a frequency of 3% in the parental amniocyte cell line, and the transformed clones expressed the fra(X) site at a frequency of 9-13%. These cell lines maintain the cytogenetic phenotype and can serve as positive controls for testing the efficacy of the inducing systems during prenatal diagnostic studies.
authors.affiliation: University of California, San Francisco.
authors.forename: L S
authors.initials: LS
authors.lastname: Jenkins
authors.forename: D C
authors.initials: DC
authors.lastname: Gruenert
authors.forename: E C
authors.initials: EC
authors.lastname: Jenkins
authors.forename: S A
authors.initials: SA
authors.lastname: Schonberg
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology; D002461:Cell Line, Transformed; D003673:Defective Viruses; D005260:Female; D005600:Fragile X Syndrome / Q000473:pathology*; D006801:Humans; D010641:Phenotype; D011296:Prenatal Diagnosis; D012015:Reference Standards; D013539:Simian virus 40