Sequence conservation of gene 8 between human and porcine group C rotaviruses and its relationship to the VP7 gene of group A rotaviruses.
cDNA libraries from porcine group (Gp) C rotavirus strain Cowden and a human Gp C rotavirus strain were generated. The complete nucleotide sequence of gene 8 from the Cowden strain was determined from gene 8-specific clones and viral transcript RNA. A full-length gene 8 clone was generated from the human Gp C virus by polymerase chain reaction (PCR) using primers deduced from the 3' and 5' ends of the Cowden strain gene 8, and the sequence of the human Gp C gene 8 was determined from this clone and gene 8 clones in the cDNA library. The gene 8 from the Cowden or the human Gp C strain is 1063 nucleotides in length and contains a long open reading frame beginning at the 49th nucleotide from the 5' end and terminating with a stop codon 16 bases from the 3' end. The encoded protein contains 332 amino acids (predicted molecular weight of 37.3 kDa) with two potential N-linked glycosylation sites in the porcine strain and three in the human strain. The polypeptide products derived from in vitro translation of the transcript RNA generated from a porcine gene 8 clone containing the entire open reading frame were analogous in size with the Gp A VP7. The gene 8 of porcine and human Gp C rotaviruses exhibited considerable nucleotide and deduced amino acid sequence identity (83.8 and 88.0%, respectively). Comparison of the Gp C gene 8 protein sequence with the VP7 protein of Gp A rotavirus revealed structural similarities, although the overall amino acid identity was low (less than 30%). These data suggest that the gene 8 of the porcine or human Gp C rotavirus encodes a protein corresponding to the VP7 outer capsid glycoprotein of Gp A rotaviruses and that the eighth gene is highly conserved in the porcine and human Gp C strains examined in this study.
Qian
Y A
YA
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Jiang
B M
BM
Saif
L J
LJ
Kang
S Y
SY
Ishimaru
Y
Y
Yamashita
Y
Y
Oseto
M
M
Green
K Y
KY
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D003001:Cloning, Molecular; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D012401:Rotavirus / Q000235:genetics*; D012995:Solubility; D013552:Swine / Q000382:microbiology; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology
D000956:Antigens, Viral; D036022:Capsid Proteins; D016601:RNA-Binding Proteins; C050779:VP7 protein, Rotavirus; D017361:Viral Nonstructural Proteins; C079234:NS35 protein, rotavirus