title: Cytoplasmic lipid bodies of human eosinophils. Subcellular isolation and analysis of arachidonate incorporation.
abstract: Lipid bodies are non-membrane-bound cytoplasmic inclusions that are prominent in leukocytes engaged in inflammatory responses. As demonstrated by electron microscopic autoradiography, lipid bodies can serve as intracellular sites of 3H-arachidonic acid localization in eosinophils and other cells. To evaluate the role of lipid bodies as stores of esterified arachidonate, subcellular fractionation of lipid-body-rich human eosinophils was used to isolate lipid bodies free of other organelles. In lipid bodies isolated from 3H-arachidonate-labeled eosinophils, 3H-arachidonate was esterified almost totally in glycerolipids, predominantly in classes of phospholipids, including phosphatidyl-inositol and phosphatidylcholine. Lipid bodies, especially in leukocytes participating in inflammation, could represent intracellular sources of esterified arachidonate available for eicosanoid formation.
authors.affiliation: Department of Medicine, Harvard Medical School, Boston, Massachusetts.
authors.forename: P F
authors.initials: PF
authors.lastname: Weller
authors.forename: R A
authors.initials: RA
authors.lastname: Monahan-Earley
authors.forename: H F
authors.initials: HF
authors.lastname: Dvorak
authors.forename: A M
authors.initials: AM
authors.lastname: Dvorak
mesh_terms: D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure*; D004804:Eosinophils / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism / Q000648:ultrastructure*; D050356:Lipid Metabolism; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D001095:Arachidonic Acids; D016718:Arachidonic Acid