title: Activation of phospholipase D: a signaling system set in motion by perturbation of the T lymphocyte antigen receptor/CD3 complex.
abstract: A number of cellular signaling systems are called into play by interaction of the T lymphocyte antigen receptor/CD3 complex with its cognate antigen. Well-described signaling systems include phosphoinositide turnover, tyrosine phosphorylation, protein kinase C activation, and increased cytosolic calcium. We have explored the possibility that another recently described signaling system, activation of phospholipase D, may be operative. Data presented here demonstrate that stimulation of Jurkat T cells with anti-CD3 antibodies or phorbol esters resulted in activation of phospholipase D, as measured by production of phosphatidylethanol and phosphatidic acid. The combination of anti-CD3 antibody plus phorbol ester led to a greater than additive production of phosphatidylethanol and to the additive production of phosphatidic acid (in the absence of ethanol). Phorbol esters as a second stimulus with anti-CD3 antibody led to a additive increase in cellular diacylglycerol content but provided no increased production of inositol phosphates, suggesting that diacylglycerol production in these cells results from hydrolysis of noninositol containing lipids as well as from phosphinositides. Exogenous addition of phosphatidic acid led to increases in cytosolic calcium that, depending on the concentration used, resulted from release of an intracellular store of calcium and influx of extracellular calcium. Changes in cytosolic calcium occurred in the absence of inositol phosphates production. These studies establish a role for increased phospholipase D activity in T lymphocyte activation.
authors.affiliation: Department of Medicine, Howard Hughes Medical Institute Vanderbilt University School of Medicine, Nashville, Tennessee.
authors.forename: S J
authors.initials: SJ
authors.lastname: Stewart
authors.forename: G R
authors.initials: GR
authors.lastname: Cunningham
authors.forename: J A
authors.initials: JA
authors.lastname: Strupp
authors.forename: F S
authors.initials: FS
authors.lastname: House
authors.forename: L L
authors.initials: LL
authors.lastname: Kelley
authors.forename: G S
authors.initials: GS
authors.lastname: Henderson
authors.forename: J H
authors.initials: JH
authors.lastname: Exton
authors.forename: S B
authors.initials: SB
authors.lastname: Bocckino
mesh_terms: D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D017252:CD3 Complex; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D004075:Diglycerides / Q000096:biosynthesis; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D008213:Lymphocyte Activation; D010712:Phosphatidic Acids / Q000494:pharmacology; D010739:Phospholipase D / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D015398:Signal Transduction / Q000276:immunology / Q000502:physiology; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D004075:Diglycerides; D010712:Phosphatidic Acids; D011948:Receptors, Antigen, T-Cell; D010739:Phospholipase D; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium