Diabetes induction and pancreatic transplantation in the cynomolgus monkey: methodological considerations.
The aim of this study was to develop a model for pancreatic transplantation in the primate in order to test a new immunosuppressive drug. Initially, streptozotocin was used to induce insulin-dependent diabetes mellitus, but it was found to be ineffective and associated with a high morbidity. Furthermore, streptozotocin-induced insulin-dependent diabetes mellitus did not always persist, thus invalidating the evaluation of pancreatic graft function. Therefore, total pancreatectomy was introduced and combined with the pancreatic allotransplantation as a single procedure. Enteric diversion of the pancreatic juice was chosen since this avoids exocrine pancreatic insufficiency and facilitates the oral administration of the test drug. Intra-arterial monitoring of blood pressure and blood gases during the operation and avoidance of hypothermia in the animal were found to be the most important factors contributing to a successful outcome from the operative procedure.
Ericzon
B G
BG
Department of Transplantation Surgery, Karolinska Institute, Huddinge Hospital, Sweden.
Wijnen
R M
RM
Kubota
K
K
vd Bogaard
A
A
Kootstra
G
G
D000818:Animals; D001786:Blood Glucose; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000601:surgery*; D004195:Disease Models, Animal; D008252:Macaca fascicularis; D016035:Pancreas Transplantation / Q000379:methods; D010180:Pancreatectomy; D013311:Streptozocin; D015996:Survival Rate
D001786:Blood Glucose; D013311:Streptozocin