Dendritic accessory cells derived from rat bone marrow precursors under chemically defined conditions in vitro belong to the myeloid lineage.
Serum-free conditions have been developed to differentiate dendritic cells from a non-adherent fraction of rat bone marrow precursors by action of the multipotential and macrophage colony-stimulating factors further supplemented with linoleic acid, vitamin E, and vitamin D3. Accessory activity was demonstrated by the high potency of the dendritic cells to stimulate autologous T cell proliferation, whereas such cells were negative for Fc receptor-dependent phagocytosis, a characteristic macrophage feature. While the dendritic cells were weakly positive for alpha-naphtylbutyrate esterase, they strongly expressed RT.1 class II antigens. Apparently, these cells represent a more differentiated phenotype since they expressed the nuclear A/C lamins. By addition of serum to the cultures, the dendritic cells developed into macrophages, which were also lamin A/C-positive as well as strongly positive for alpha-naphtylbutyrate esterase. Thus, these dendritic cells belong to the myeloid lineage, and it appears as if serum factor(s) control differentiation at a mature level. Suitable conditions could also be established for large-scale cultures of dendritic cells, which would be useful for applications requiring higher numbers of cells.
Gieseler
R K
RK
Department of Immunology, Georg August University of Göttingen, Federal Republic of Germany.
Röber
R A
RA
Kuhn
R
R
Weber
K
K
Osborn
M
M
Peters
J H
JH
D000818:Animals; D001854:Bone Marrow Cells; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D002762:Cholecalciferol / Q000378:metabolism; D003470:Culture Media; D003713:Dendritic Cells / Q000166:cytology*; D005227:Fatty Acids / Q000378:metabolism; D007700:Kinetics; D034904:Lamin Type A; D034882:Lamins; D008213:Lymphocyte Activation; D008264:Macrophages / Q000166:cytology; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism
D003470:Culture Media; D005227:Fatty Acids; D034904:Lamin Type A; D034882:Lamins; D009687:Nuclear Proteins; D014810:Vitamin E; D002762:Cholecalciferol; D002265:Carboxylic Ester Hydrolases; C021855:naphthylbutyrate esterase