title: Two-dimensional NMR study of the conformation of (34-65)bacterioopsin polypeptide in SDS micelles.
abstract: The conformation of the synthetic 32-residue polypeptide, an analog of the membrane spanning segment B (residues 34-65) of Halobacterium halobium bacterioopsin, incorporated into perdeuterated sodium dodecyl sulfate micelles in the presence of trifluoroethanol was investigated by 1H NMR spectroscopy. The spectrum resonances were assigned by means of phase-sensitive DQF-COSY, TOCSY and NOESY techniques. Interproton nuclear Overhauser effects and deuterium exchange rates of individual NH groups were derived from two-dimensional NMR spectra. Analysis of the obtained data showed that segment B has a right-handed alpha-helical stretch from Lys41 to Leu62 with a kink at Pro50. The alpha-helix in the C-terminal part is terminated at Gly63, which adopts a conformation typical of amino acid residues in a left-handed helix. The N-terminal part (residues 34-40) has no ordered conformation. NMR data are provided for comparison of the segment B conformation in the isotropic system of an organic solvent, in SDS micelles and in the purple membrane bacterioopsin. Factors affecting the conformation of membrane spanning segment B in various milieus are discussed.
authors.affiliation: M.M. Shemyakin Institute of Bioorganic Chemistry, U.S.S.R. Academy of Sciences, Moscow.
authors.forename: K V
authors.initials: KV
authors.lastname: Pervushin
authors.forename: A S
authors.initials: AS
authors.lastname: Arseniev
authors.forename: A T
authors.initials: AT
authors.lastname: Kozhich
authors.forename: V T
authors.initials: VT
authors.lastname: Ivanov
mesh_terms: D000595:Amino Acid Sequence; D019617:Halobacterium salinarum / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008823:Micelles; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation; D012967:Sodium Dodecyl Sulfate
chemical_list: D008823:Micelles; D010455:Peptides; C077768:bacterioopsin(34-65) polypeptide; D012967:Sodium Dodecyl Sulfate