The fate and possible role of arylphorin during the metamorphosis of Mamestra brassicae.
1. Arylphorin, one of the storage proteins has been isolated from the hemolymph of Mamestra brassicae. 2. It has been established that Mamestra arylphorin is the most similar to manducin from among the known storage proteins of other species. 3. A rabbit polyclonal antibody has been developed against arylphorin, and its concentration changes have been determined quantitatively by ELISA in the hemolymph and fat body from the 1st day of the last larval instar to the 3rd day of the imago stage. 4. Histological sections were made on each day during the investigated period and it was shown by immunohistochemical methods that the main quantity of arylphorin was accumulated in the storage protein granules of the fat body and it could be detected even in the imaginal fat body. 5. The uptake of arylphorin by the fat body is induced by 20-hydroxyecdysone. 6. During differentiation of the imaginal cuticle arylphorin is incorporated first in the epidermal cells and it is built in the endocuticular layer of the integument thereafter.
Sass
M
M
Department of General Zoology, Eötvös Loránd University, Budapest, Hungary.
Low
P
P
Móricz
Z
Z
Csikós
G
G
Kovács
J
J
D000818:Animals; D015153:Blotting, Western; D002850:Chromatography, Gel; D004441:Ecdysterone / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005216:Fat Body / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins; D006458:Hemolymph / Q000737:chemistry / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007301:Insect Hormones / Q000302:isolation & purification / Q000502:physiology*; D019476:Insect Proteins; D008675:Metamorphosis, Biological; D009036:Moths / Q000254:growth & development*; D011817:Rabbits
D006023:Glycoproteins; D007301:Insect Hormones; D019476:Insect Proteins; C041137:storage proteins, Insecta; D004441:Ecdysterone