Effect of protease inhibitors on DNA amplification in SV40-transformed Chinese hamster embryo cells.
We have examined the effect of protease inhibitors on radiation-induced DNA amplification in vitro using an SV40-transformed Chinese hamster embryo cell line. DNA from cells irradiated with X-rays (10 Gy) or ultraviolet radiation (8 J/m2) resulted in an 8-fold or greater amplification of SV40 sequences compared with unirradiated cells. Addition of antipain or the soybean-derived Bowman-Birk protease inhibitor (BBI) to the culture medium, either 75 min after X-irradiation or within 15 min after ultraviolet irradiation, resulted in a reduction of amplification levels. BBI was more effective than antipain at an equal concentration. These results suggest that a protease may be involved in DNA amplification.
Flick
M B
MB
Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia 19104.
Kennedy
A R
AR
D000818:Animals; D000976:Antipain / Q000494:pharmacology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D003412:Cricetulus; D004279:DNA, Viral / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects; D004622:Embryo, Mammalian; D005784:Gene Amplification / Q000187:drug effects*; D013539:Simian virus 40 / Q000235:genetics*; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000494:pharmacology*; D014466:Ultraviolet Rays; D014965:X-Rays
D004279:DNA, Viral; D014358:Trypsin Inhibitor, Bowman-Birk Soybean; D000976:Antipain