Receptor sequence in the terminal protein of bacteriophage M2 that interacts with an RGD (Arg-Gly-Asp) sequence of the primer protein.
At the initiation of protein-primed DNA replication of bacteriophages M2 and phi 29, the Arg-Gly-Asp (RGD) sequence of primer protein participates in the recognition of terminal protein (TP), where the initiation site for protein-primed DNA replication of template DNA is located. We compared the sequences of M2 and phi 29 TP with those of the members of the integrin superfamily and found the highly homologous sequences Lys-Lys-Ile-Pro-Pro-Asp-Asp (KKIPPDD) in M2 and phi 29 TP and Lys-Lys-Gly-Cys-Pro-Pro-Asp-Asp (KKGCPPDD) in the beta-subunit of fibronectin receptor protein. A synthetic 20mer peptide that contained the KKIPPDD sequence interfered with the inhibitory effect of the RGD peptide on both transfection and the protein-priming reaction in vitro. We propose that the sequence KKIPPDD of M2 TP is the receptor sequence for RGD.
Kobayashi
H
H
Life Science Institute, Sophia University, Tokyo, Japan.
Kitabayashi
K
K
Matsumoto
K
K
Hirokawa
H
H
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D016023:Integrins / Q000737:chemistry; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000737:chemistry; D014162:Transfection / Q000187:drug effects; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism*
D004268:DNA-Binding Proteins; D016023:Integrins; D009842:Oligopeptides; D010446:Peptide Fragments; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic; D014764:Viral Proteins; C068917:primer protein, Bacteriophage M2; C071904:terminal protein, Bacteriophage M2; C047981:arginyl-glycyl-aspartic acid