title: Isolation, characterization and expression of a gene coding for a 2S albumin from Bertholletia excelsa (Brazil nut).
abstract: Two genes, BE2S1 and BE2S2, coding for methionine-rich albumins of Brazil nut (Bertholletia excelsa H.B.K.) have been cloned and their sequence determined. The genes are members of a multigene family and one of them, i.e. BE2S1, codes for one of the dominant 2S isoforms. Its expression is highly regulated during seed development and with respect to tissue specificity. Sequence analysis has shown that the genes contain one intron and that the promoter of BE2S1 shows a canonical TATA motif. The transcription initiation site is located 26 nucleotides downstream from the TATA box. Sequence comparison of the promoter regions of 2S genes from Brassica napus, Arabidopsis thaliana and B. excelsa revealed the presence of TGCA palindromic sequence that appear to be arranged in a 2S-specific manner.
authors.affiliation: S.A.I.N., Brasilia-DF, Brazil.
authors.forename: E S
authors.initials: ES
authors.lastname: Gander
authors.forename: K O
authors.initials: KO
authors.lastname: Holmstroem
authors.forename: G R
authors.initials: GR
authors.lastname: De Paiva
authors.forename: L A
authors.initials: LA
authors.lastname: De Castro
authors.forename: M
authors.initials: M
authors.lastname: Carneiro
authors.forename: M F
authors.initials: MF
authors.lastname: Grossi de Sá
mesh_terms: D055516:2S Albumins, Plant; D000418:Albumins / Q000235:genetics*; D000485:Allergens; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000502:physiology; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009754:Nuts / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012639:Seeds / Q000254:growth & development; D016415:Sequence Alignment; D016385:TATA Box / Q000235:genetics
chemical_list: D055516:2S Albumins, Plant; D000418:Albumins; D000485:Allergens; D052179:Antigens, Plant; D010940:Plant Proteins; C103364:2S albumin, brazil nut