Expression of mouse serotonin transporter RNA in Xenopus laevis oocytes.
The serotonin transporter removes serotonin from the synapse, terminating the action of this neurotransmitter. We have taken an initial step towards cloning the serotonin transporter by obtaining its expression in Xenopus oocytes. We found that mouse brainstem is a suitable source of serotonin transporter mRNA and that synthesis of the transporter in injected oocytes occurs steadily over a 3 day period. Uptake of [3H] serotonin was inhibited by 18 nM citalopram, a specific inhibitor of serotonin transport.
Haber
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R
Laboratory of Clinical Studies, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892.
Goldman
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D
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000378:metabolism; D015283:Citalopram / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000378:metabolism; D026901:Membrane Transport Proteins; D051379:Mice; D009419:Nerve Tissue Proteins; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*; D050486:Serotonin Plasma Membrane Transport Proteins; D014982:Xenopus laevis
D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D050486:Serotonin Plasma Membrane Transport Proteins; C493231:Slc6a4 protein, mouse; D015283:Citalopram; D012701:Serotonin