Identification of enterobactin and linear dihydroxybenzoylserine compounds by HPLC and ion spray mass spectrometry (LC/MS and MS/MS).
The production of catecholate siderophores was studied in some selected species of Enterobacter (Enterobacteriaceae). The extracted catecholates were separated as iron-free compounds by HPLC on a C18 reversed-phase column using methanol/0.1% phosphoric acid or methanol/0.1% formic acid as a solvent system and identified by ion spray mass spectrometry (LC/MS, MS/MS). Five catecholate compounds were identified which include 2,3-dihydroxybenzoylserine, its linear dimer and trimer, the cyclic enterobactin and an unidentified isomer of enterobactin. In addition, a new large-scale method for the isolation of catecholate siderphores is described which is based on adsorption on XAD-2 and subsequent purification on Sephadex LH20.
Berner
I
I
Institut für Biologie, Mikrobiologie/Biotechnologie, Universität Tübingen, Federal Republic of Germany.
Greiner
M
M
Metzger
J
J
Jung
G
G
Winkelmann
G
G
D001704:Biopolymers; D002851:Chromatography, High Pressure Liquid; D004755:Enterobacteriaceae / Q000032:analysis*; D004758:Enterobactin / Q000032:analysis*; D007536:Isomerism; D013058:Mass Spectrometry / Q000379:methods; D012694:Serine / Q000031:analogs & derivatives* / Q000032:analysis
D001704:Biopolymers; D004758:Enterobactin; D012694:Serine; C031920:2,3-dihydroxybenzoylserine