Immunohistochemical localization of beta-adrenergic receptors in the heart.
Polyclonal specific antisera to beta-receptors were prepared by immunization of rabbits with beta-receptors isolated from a dog myocardium according to Becker et al. (1988). Cryostat sections of the dog heart were fixed in paraformaldehyde in PBS, and incubated with polyclonal antisera to beta-receptors. Sections were then washed and incubated in peroxidase-labelled anti-rabbit antibody. After visualization of the tissue-bound peroxidase with diaminobenzidine, sections were dehydrated in graded alcohols and mounted in Canada balsam. The results demonstrate specific reaction of antibodies on the myocyte sarcolemma.
Rada
T
T
Institute for Heart Research, Slovak Academy of Sciences, Bratislava.
Okruhlicová
L
L
Slezák
J
J
D000818:Animals; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D009206:Myocardium / Q000378:metabolism* / Q000648:ultrastructure; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D012508:Sarcolemma / Q000378:metabolism
D011943:Receptors, Adrenergic, beta