Characterization and purification of the neuronal sodium-ion-coupled 5-hydroxytryptamine transporter.
The selective 5-hydroxytryptamine (5-HT) uptake inhibitor, [3H]paroxetine, has been used as a specific probe in a series of experiments aimed at characterizing the substrate and 5-HT uptake inhibitor binding domains on the neuronal sodium-ion-coupled 5-HT transporter. In addition, an affinity-chromatographic protocol which provided a greater than 3000-fold purification of this neuronal transporter protein is outlined.
Graham
D
D
Department of Biology, Synthélabo Recherche (L.E.R.S.), Paris, France.
Esnaud
H
H
Langer
S Z
SZ
D000818:Animals; D001667:Binding, Competitive; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D017374:Paroxetine; D010880:Piperidines / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism* / Q000494:pharmacology
D002352:Carrier Proteins; D010880:Piperidines; C025366:serotonin-binding protein; D012701:Serotonin; D017374:Paroxetine; D012964:Sodium