Genomic DNA sequences of two new genes for new storage protein glutelin in rice.
A new cDNA and two genomic genes encoding the rice storage protein glutelin were isolated and sequenced. The nucleotide sequence of one gene (GluA-3) was completely identical with that of the new cDNA identified here, and the other (GluA-4) was a pseudogene. These glutelin genes were closely related to each other, and belonged to the subfamily A containing the type I (GluA-1) and II (GluA-2) glutelin genes. The Northern blot analysis, using synthetic oligonucleotide specific to the GluA-3 gene as a probe, showed that this gene was expressed earlier than other glutelin genes during seed maturation.
Takaiwa
F
F
Department of Cell Biology, National Institute of Agrobiological Resources, Ibaraki.
Oono
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005983:Glutens / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D012275:Oryza / Q000235:genetics*; D011544:Pseudogenes; D014158:Transcription, Genetic
D005983:Glutens; D004247:DNA