Demonstration of fructose 1,6-bisphosphatase in human term placenta.
A proteolysed form of fructose 1,6-bisphosphatase (Fru-P2ase) has been detected and characterized in human term placenta. The extract was found to contain very low levels of activity with an alkaline pH optimum. Western blotting demonstrated a polypeptide of Mr 26,000, instead of the subunit of Mr 36,000 observed in native mammalian Fru-P2ases.
Papamarcaki
T
T
Laboratory of Biological Chemistry, University of Ioannina Medical School, Greece.
Tsolas
O
O
D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006597:Fructose-Bisphosphatase / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy
D046911:Macromolecular Substances; D006597:Fructose-Bisphosphatase