title: Relationship between a subunit of NADH dehydrogenase (complex I) and a protein family including subunits of cytochrome reductase and processing protease of mitochondria.
abstract: The primary structure of a 40 kDa subunit of the respiratory chain NADH:ubiquinone reductase from Neurospora crassa was determined by sequencing cDNA, genomic DNA and the N-terminus of the mature protein. The gene which is interrupted by 7 introns encodes a preprotein consisting of 375 amino acids with a 26 amino acid long presequence typical for a mitochondrial targeting signal. The sequence of the mature subunit shows conspicuous similarities to the recently [(1989) Nature 339, 147-149] discovered protein family which includes subunits I and II of the ubiquinol:cytochrome c reductase, and the processing proteins, matrix processing peptidase and processing enhancing protein, of mitochondria. The possible role of the subunit is discussed.
authors.affiliation: Institut für Biochemie, Universität Düsseldorf, Germany.
authors.forename: D A
authors.initials: DA
authors.lastname: Röhlen
authors.forename: J
authors.initials: J
authors.lastname: Hoffmann
authors.forename: J C
authors.initials: JC
authors.lastname: van der Pas
authors.forename: U
authors.initials: U
authors.lastname: Nehls
authors.forename: D
authors.initials: D
authors.lastname: Preis
authors.forename: U
authors.initials: U
authors.lastname: Sackmann
authors.forename: H
authors.initials: H
authors.lastname: Weiss
mesh_terms: D000595:Amino Acid Sequence; D003579:Cytochrome Reductases / Q000235:genetics*; D004247:DNA / Q000235:genetics; D010450:Endopeptidases / Q000235:genetics*; D007438:Introns; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D009492:Neurospora crassa; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004247:DNA; D003579:Cytochrome Reductases; D009245:NADH Dehydrogenase; D010450:Endopeptidases