Herpes simplex virus type 1 long-term persistence, latency, and reactivation in infected Burkitt lymphoma cells.
The two herpes simplex virus type 1 (HSV-1) strains F and AK which differ in virus-cell interaction and in DNA organization, were used to establish persistently productive infections in Burkitt lymphoma-derived cell lines BJAB and Raji. Four such lines could be maintained over a period of three years. Like the uninfected parental lines, the persistently infected cells display a cyclic pattern of cell proliferation. The expression of HSV-1-specific antigens proved to be variable. As a consequence, virus yields also vary within a subcultivation period. Pooled human HSV antisera, when continuously present, suppress virus production (inducible latency) and support cell proliferation to higher rates. By contrast, removal of the antiserum after a certain period of cultivation leads to virus reactivation with a delay of 8 to 20 days. After cultivation periods of more than 3 to 12 weeks, replacement of HSV antiserum does no longer result in virus reactivation and even inducers fail to reactivate.
Hampl
W
W
Abteilung Virologie, Universität Ulm, Federal Republic of Germany.
Conrad
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S
Kleinschmidt
A K
AK
D000914:Antibodies, Viral / Q000494:pharmacology; D002051:Burkitt Lymphoma; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000096:biosynthesis; D015245:Deoxyribonuclease BamHI; D005455:Fluorescent Antibody Technique; D015183:Restriction Mapping; D018139:Simplexvirus / Q000235:genetics / Q000254:growth & development*; D013045:Species Specificity; D013997:Time Factors; D014407:Tumor Cells, Cultured; D010948:Viral Plaque Assay; D014775:Virus Activation; D014776:Virus Cultivation; D014779:Virus Replication
D000914:Antibodies, Viral; D004279:DNA, Viral; D015245:Deoxyribonuclease BamHI