Quantum yield of CHAPSO-solubilized rhodopsin and 3-hydroxy retinal containing bovine opsin.
The quantum yields of bleaching for two artificial pigments, bovine opsin combined with (3R)-3-hydroxy retinal or (3R,S)-3-methoxy retinal, were determined in comparison to the value for regenerated bovine rhodopsin. Regeneration of the visual pigments was performed by incubation of 3-[(3-Cholamidopropyl)-dimethylammonio]-2-hydroxy-1- propanesulfonate (CHAPSO)-solubilized opsin with the 11-cis isomers of retinal and the respective retinal derivatives. The extinction coefficients of the pigments in CHAPSO were determined to 35,000 M-1 cm-1 (native rhodopsin), 35,300 M-1 cm-1 (regenerated rhodopsin) and 34,500 M-1 cm-1 (3-OH retinal opsin). With respect to rhodopsin (lambda max: 500 nm), the pigments carrying the substituted chromophores exhibit blue shifted absorbance maxima (3-hydroxy and 3-methoxy retinal opsin: 488 nm). In parallel experiments under absolutely identical conditions we find related to the value of CHAPSO solubilized rhodopsin (identical to 1) a quantum efficiency of bleaching for the 3-hydroxy pigment of 1.2.
Gärtner
W
W
Institut für Biologie I (Zoologie), Universität Freiburg, Fed. Rep. Germany.
Ullrich
D
D
Vogt
K
K
D000818:Animals; D002417:Cattle; D002793:Cholic Acids; D003902:Detergents; D005136:Eye Proteins / Q000737:chemistry* / Q000378:metabolism; D011789:Quantum Theory; D012172:Retinaldehyde / Q000031:analogs & derivatives* / Q000378:metabolism*; D012243:Rhodopsin / Q000737:chemistry* / Q000378:metabolism; D012374:Rod Cell Outer Segment / Q000378:metabolism*; D017299:Rod Opsins; D012995:Solubility; D013053:Spectrophotometry
D002793:Cholic Acids; D003902:Detergents; D005136:Eye Proteins; D017299:Rod Opsins; C048531:chapso; D012243:Rhodopsin; C049427:3-hydroxyretinal; D012172:Retinaldehyde