An exon 5-deleted mRNA encodes a functional interleukin 2 receptor alpha-subunit.
Two polypeptides are involved in interleukin 2 binding: a low-affinity receptor of 55 kD (IL2-R alpha) and an intermediate affinity component of 75 kD (IL2-R beta). We describe the cloning by the Polymerase Chain Reaction of the coding region of IL2-R alpha from a human T-cell lymphoma cell line. One clone presented a 72-bp deletion that precisely corresponds to exon 5. The deleted form and the normal IL2-R alpha cDNA were expressed CHO cells. Stable transfected cellular clones were compared for their immunoreactivity to monoclonal antibodies directed against IL2-R alpha and for their ability to bind radiolabeled IL2. The presence or absence of the protein region encoded by exon 5 did not modify the IL2-binding capacity of the receptor.
Mercken
L
L
Rhône-Poulenc Rorer, C.R.V.A., Vitry-sur-Seine, France.
Moras
V
V
Hemon
L
L
Lionne
B
B
Bousseau
A
A
Dautry-Varsat
A
A
Collins
M
M
Mayaux
J F
JF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002460:Cell Line; D002872:Chromosome Deletion; D002999:Clone Cells; D006224:Cricetinae; D005091:Exons; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D016399:Lymphoma, T-Cell; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D016133:Polymerase Chain Reaction; D015375:Receptors, Interleukin-2 / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D014162:Transfection
D007376:Interleukin-2; D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D015375:Receptors, Interleukin-2