title: Selenoprotein A component of the glycine reductase complex from Clostridium purinolyticum: nucleotide sequence of the gene shows that selenocysteine is encoded by UGA.
abstract: The gene encoding the selenoprotein A component of glycine reductase was isolated from Clostridium purinolyticum. The nucleotide sequence of this gene (grdA) was determined. The opal termination codon (TGA) was found in-frame at the position corresponding to the location of the selenocysteine residue in the gene product. A comparison of the nucleotide sequences and secondary mRNA structures corresponding to the selenoprotein A gene and the fdhF gene of Escherichia coli formate dehydrogenase shows that there is a similar potential for regulation of the specific insertion of selenocysteine at the UGA codon.
authors.affiliation: Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: G E
authors.initials: GE
authors.lastname: Garcia
authors.forename: T C
authors.initials: TC
authors.lastname: Stadtman
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003013:Clostridium / Q000235:genetics*; D003062:Codon; D003545:Cysteine / Q000031:analogs & derivatives* / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000235:genetics*; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000648:ultrastructure; D015183:Restriction Mapping; D012643:Selenium; D017279:Selenocysteine; D051140:Selenoproteins
chemical_list: D001426:Bacterial Proteins; D003062:Codon; D009097:Multienzyme Complexes; D012333:RNA, Messenger; D051140:Selenoproteins; C054667:selenoprotein A; D017279:Selenocysteine; D000594:Amino Acid Oxidoreductases; C020815:glycine reductase; D012643:Selenium; D003545:Cysteine