title: DNA sequence of the nucleoside triphosphate phosphohydrolase I (NPH I) of the Choristoneura biennis entomopoxvirus.
abstract: The DNA sequence of an open reading frame (ORF) corresponding to a Choristoneura biennis entomopoxvirus putative late gene was determined. Residing within an 8-kp EcoRI viral genomic fragment, this ORF is 1944 nucleotides long, encoding a basic protein (pI 9.83) with a predicted molecular weight of 76,000 Da. Computer analysis indicates a 36.4% homology between this ORF and the vaccinia nucleoside triphosphate phosphohydrolase I (NHP I) gene, with substantially greater homology (60%) in two domains believed to be involved in ATP binding. The entomopoxvirus ORF contains 78% AT residues; and like other poxvirus late genes, it possesses the conserved TAAAT motif at the 5' terminus of the gene.
authors.affiliation: Genetic Engineering Section, National Research Council Canada, Montreal, Quebec.
authors.forename: L
authors.initials: L
authors.lastname: Yuen
authors.forename: M
authors.initials: M
authors.lastname: Noiseux
authors.forename: M
authors.initials: M
authors.lastname: Gomes
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005814:Genes, Viral; D007304:Insect Viruses / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D043583:Nucleoside-Triphosphatase; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D011212:Poxviridae / Q000201:enzymology / Q000235:genetics*; D015183:Restriction Mapping; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins; D015678:Viral Structural Proteins; D010744:Phosphoric Monoester Hydrolases; D043583:Nucleoside-Triphosphatase