Regulation of expression of glial filament acidic protein.
The regulation of cell type-specific expression of the gene encoding glial filament acidic protein (GFAP) was examined by introducing various deletion mutants of the gene into GFAP-expressing (U251 human astrocytoma) and non-expressing (HeLa) cell lines, and measuring their transcriptional activity in an RNAase protection assay. The expression of GFAP is influenced by a number of cis-acting elements. A domain that resides between nucleotides -1631 and -1479 can confer cell type-specific expression when coupled to a heterologous gene. We also present evidence for the existence of a negative regulatory element that resides within the first intron of the GFAP gene.
Sarkar
S
S
Department of Biochemistry, NYU Medical Center, NY 10016.
Cowan
N J
NJ
D000818:Animals; D005786:Gene Expression Regulation; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D009457:Neuroglia / Q000378:metabolism; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D005904:Glial Fibrillary Acidic Protein