[Factors affecting the results of culture of Chlamydia trachomatis in a McCoy cell culture].
The authors confirmed that the sensitivity of cultivation of the laboratory strain Chlamydia trachomatis, serotype E in a McCoy cell culture is increased by centrifuging of the inoculum and addition of cycloximidine to the cultivation medium. Conversely, postcentrifugation sedimentation of the inoculum and addition of glucose to the medium has no effect. To meet the needs of the parasite and the host cells the glucose content in the basic p component of the medium--MEM is sufficient. Exchange of the basic constituent of the medium--MEM for RPMI 1640 did not prove useful in the cultivation of the laboratory strain of chlamydia. In cell cultures where medium with RPMI 1640 was used chlamydia inclusions were not found.
Stefánik
M
M
Vojenský ústav hygieny, epidemiológie a mikrobiológie, Praha.
Valkoun
A
A
D001431:Bacteriological Techniques; D002478:Cells, Cultured; D002692:Chlamydia trachomatis / Q000254:growth & development*
Faktory ovplyvnujúce výsledok kultivácie Chlamydia trachomatis v kultúre buniek McCoy.