Characterization of the Pseudomonas aeruginosa transposable phage D3112 [corrected] left-end regulatory region.
The nucleotide sequence (2682 bp) of the left end of the Mu-like transposable bacteriophage D3112 cts15 from Pseudomonas aeruginosa was determined. A 720 bp open reading frame (ORF) is located on the bottom strand (positions 892-173), potentially encoding a polypeptide of 240 residues (Mr = 26,329). Specific binding of Escherichia coli Integration Host Factor (IHF) to a site located 907-922 bp from the D3112 left end suggests the existence of a P. aeruginosa IHF and its role, as in Mu, in the regulation of phage development.
Autexier
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Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada.
Wragg-Légaré
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DuBow
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D001426:Bacterial Proteins / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005809:Genes, Regulator; D005814:Genes, Viral; D034982:Integration Host Factors; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D034982:Integration Host Factors; C440503:integration host factor, Pseudomonas