title: Typing of heat-stable soluble Haemophilus parasuis antigen by means of agargel precipitation and the dot-blot procedure.
abstract: According to Morozumi's and Nicolet's (1986) investigations, a serological classification procedure for H. parasuis and to a certain extent, for H. parasuis-like strains was proposed on the basis of heat-stable cell antigens in the immunodiffusion test. It was possible to classify 72.8% of the investigated strains serologically using this procedure. 7 of 12 serotypes were described for the first time. 60.1% of the classified strains belonged to the already known serotypes SV 1 to SV 5, whereas the new serotypes SV Jena 6 to SV Jena 12 amounted to only 12.7% of the field isolates. The serotypes SV Jena 7 to 9 are represented by H. parasuis-like strains. Unencapsulated strains and isolates of serotype SV 5 dominate in animals with Glasser's disease. The serotypes SV 1, SV 5, Jena 6 and SV Jena 10 proved to be highly virulent in SPF pigs, SV 2 and SV 4 were of medium virulence. The other serotypes were non-virulent. The high specificity in AGPT was not reproducible in the more sensitive dot-blot procedure. This must be taken into account, if the dot-blot is to be used for the classification of serotypes of H. parasuis. The results point to a participation of nonimmunogenic polysaccharides in the detection reaction.
authors.affiliation: Academy of Agricultural Sciences, Research Institute of Bacterial Animal Diseases Jena.
authors.forename: P
authors.initials: P
authors.lastname: Kielstein
authors.forename: H
authors.initials: H
authors.lastname: Rosner
authors.forename: W
authors.initials: W
authors.lastname: Müller
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D006190:Haemophilus / Q000145:classification / Q000276:immunology*; D015151:Immunoblotting; D005779:Immunodiffusion; D012703:Serotyping; D013047:Specific Pathogen-Free Organisms; D013552:Swine
chemical_list: D000942:Antigens, Bacterial