Human interferon omega (omega) binds to the alpha/beta receptor.
It was proposed that human interferon omega (omega) binds to the interferon alpha/beta receptor but not to the interferon gamma receptor. However, since no studies were performed to provide direct evidence for this hypothesis, we carried out cross-linking experiments and saturation binding assays between a 32P-labeled human interferon-alpha (Hu-IFN-alpha) and unlabeled Hu-IFN-alpha A, -beta, -gamma, and -omega. These assays demonstrated that Hu-IFN-alpha A, -beta, and -omega, but not Hu-IFN-gamma, were able to block binding of 32P-labeled Hu-IFN-alpha A to human cells. These results indicate that Hu-IFN-omega binds to the alpha/beta receptor.
Flores
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Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway 08854-5635.
Mariano
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Pestka
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D001667:Binding, Competitive; D002478:Cells, Cultured; D003432:Cross-Linking Reagents; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism*; D017471:Receptors, Interferon
D003432:Cross-Linking Reagents; D007370:Interferon Type I; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; C063743:interferon omega 1