Multiple forms of dynamin are encoded by shibire, a Drosophila gene involved in endocytosis.
Dynamin was discovered in bovine brain tissue as a nucleotide-sensitive microtubule-binding protein of relative molecular mass 100,000. It was found to cross-link microtubules into highly ordered bundles, and appeared to have a role in intermicrotubule sliding in vitro. Cloning and sequencing of rat brain dynamin complementary DNA identified an N-terminal region of about 300 amino acids which contained the three consensus elements characteristic of GTP-binding proteins. Extensive homology was found between this domain and the mammalian Mx proteins which are involved in interferon-induced viral resistance, and with the product of the VPS1 locus in Saccharomyces cerevisiae, which has been implicated both in membrane protein sorting, and in meiotic spindle pole separation. Dynamin-containing microtubule bundles were not observed in an immunofluorescence study of cultured mammalian cells, but a role for a GTP-requiring protein in intermicrotubule sliding during mitosis in plants has been reported. We report here that Drosophila melanogaster contains multiple tissue-specific and developmentally-regulated forms of dynamin, which are products of the shibire locus previously implicated in endocytic protein sorting.
Chen
M S
MS
Cell Biology Group, Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.
Obar
R A
RA
Schroeder
C C
CC
Austin
T W
TW
Poodry
C A
CA
Wadsworth
S C
SC
Vallee
R B
RB
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D017301:Ca(2+) Mg(2+)-ATPase / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D003429:Cross Reactions; D004247:DNA / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D034281:Dynamins; D004705:Endocytosis; D005796:Genes; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D051381:Rats
D029721:Drosophila Proteins; D004247:DNA; D017301:Ca(2+) Mg(2+)-ATPase; D034281:Dynamins; C439628:shi protein, Drosophila