Phospholipase C-gamma 1 directly associates with the p70 trk oncogene product through its src homology domains.
Tyrosine phosphorylation of proteins was examined in NIH3T3 cells transformed by an oncogenic form of the trk protein. Proteins of 148, 140, 70, and 55 kDa were phosphorylated on tyrosine residues in trk-transformed cells but not control NIH3T3 cells. The 70-kDa protein may represent the trk oncogene protein itself which was shown to be tyrosine-phosphorylated in vivo using trk-specific antiserum. Phospholipase C-gamma 1 (PLC-gamma 1) was also found to be constitutively tyrosine-phosphorylated in trk-transformed cells and the trk protein co-immunoprecipitated with PLC-gamma. The GTPase-activating protein of ras (GAP) and the 62-kDa GAP-associated protein were tyrosine-phosphorylated in trk-transformed cells, and a lesser amount of trk co-immunoprecipitated with GAP relative to with PLC-gamma. The trk oncogene product bound specifically to a bacterially expressed fusion protein containing the src homology domains of PLC-gamma. The data suggest a significant role for PLC-gamma in intracellular signaling by the trk oncogene.
Ohmichi
M
M
Department of Physiology, University of Michigan, Ann Arbor 48109.
Decker
S J
SJ
Pang
L
L
Saltiel
A R
AR
D015153:Blotting, Western; D002461:Cell Line, Transformed; D004591:Electrophoresis, Polyacrylamide Gel; D015688:Oncogene Protein pp60(v-src) / Q000378:metabolism*; D015513:Oncogene Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011233:Precipitin Tests; D021382:Protein Sorting Signals / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D010738:Type C Phospholipases / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
D015513:Oncogene Proteins; D021382:Protein Sorting Signals; C065400:oncogene protein trk; D014443:Tyrosine; D015688:Oncogene Protein pp60(v-src); D010738:Type C Phospholipases