Evidence that a protein kinase enhances amsacrine mediated formation of topoisomerase II-DNA complexes in murine mastocytoma cell nuclei.
Cytoplasmic extracts of K21 murine mastocytoma cells contain a protein factor, distinct from topoisomerases I and II, that facilitates formation of amsacrine-induced topoisomerase II-DNA complexes (PDC) in isolated K21 cell nuclei (Darkin, S.J. and Ralph, R.K. (1988) Biochim. Biophys. Acta 1007, 295-300). The PDC enhancing activity was shown to reside in a protein kinase with specificity for a casein kinase II substrate and sensitive to heparin and anti-casein kinase II antiserum. This appears to be the first direct evidence of a protein factor that modulates amsacrine-induced topoisomerase II action.
Darkin-Rattray
S J
SJ
Department of Cellular and Molecular Biology, University of Auckland, New Zealand.
Ralph
R K
RK
D000677:Amsacrine / Q000494:pharmacology*; D000818:Animals; D047388:Casein Kinases; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D004250:DNA Topoisomerases, Type II / Q000302:isolation & purification / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000302:isolation & purification / Q000378:metabolism*; D007106:Immune Sera; D007700:Kinetics; D012515:Mast-Cell Sarcoma; D051379:Mice; D011485:Protein Binding; D011494:Protein Kinases / Q000276:immunology / Q000378:metabolism*; D012513:Sarcoma, Experimental
D004273:DNA, Neoplasm; D007106:Immune Sera; D000677:Amsacrine; D011494:Protein Kinases; D047388:Casein Kinases; D004250:DNA Topoisomerases, Type II