Does the difference of the properties of trisaccharide-BSA conjugate (NT-P-BSA) of Mycobacterium leprae phenolic glycolipid influence on its seroreactivity?
The sugar content of the trisaccharide-BSA conjugate of the phenolic glycolip I of Mycobacterium leprae (NT-P-BSA) increased with the increase of the molar ratio of the trisaccharide to BSA used in the coupling reaction. The difference of the sugar content in NT-P-BSA did not give the influence on the seroreactivity and specificity in ELISA for both IgM and IgG class antibodies. During the course of the coupling reaction, about half amount of BSA was converted to dimeric form. However, there were no differences of the activity and specificity between monomeric form and dimeric form of NT-P-BSA. Based on these results, it was concluded that any lot of NT-P-BSA with variety of sugar content can be used in ELISA without any difference of the seroreactivity.
Fujiwara
T
T
Institute for Natural Science, Nara University, Japan.
Izumi
S
S
Wu
Q
Q
D000942:Antigens, Bacterial; D002241:Carbohydrates / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006017:Glycolipids / Q000032:analysis; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007918:Leprosy / Q000175:diagnosis; D008970:Molecular Weight; D012698:Serologic Tests; D012710:Serum Albumin, Bovine / Q000276:immunology
D000942:Antigens, Bacterial; D002241:Carbohydrates; D006017:Glycolipids; D007070:Immunoglobulin A; D007075:Immunoglobulin M; C045169:phenolic glycolipid I, Mycobacterium leprae; D012710:Serum Albumin, Bovine