title: Cytotoxic effects of resin components on cultured mammalian fibroblasts.
abstract: The objectives of this study were to determine the cytotoxic concentrations of 11 components of resin composites on monolayers of cultured Balb/c 3T3 fibroblasts, to study the inhibitory effects of these components on DNA synthesis, total protein content, and protein synthesis, and to determine whether effects were reversible when the components were withdrawn from the medium. These data were reported as concentrations which inhibited 10% (ID10) and 50% (ID50) of a particular metabolic process as well as the range of concentrations over which cell metabolism was irreversibly inhibited. For any individual component, the ID50 values for all three metabolic parameters were of the same magnitude. The same was true for the ranges of irreversibility. Ethoxylated Bis-phenol A dimethacrylate (E-BPA) was the most toxic molecule of the group (ID50 being between 1 and 10 mumol/L). The ID50 concentrations for three of the components, including Bis-GMA, UDMA, TEGDMA, and Bis-phenol A, ranged between 10 and 100 mumol/L, while the ID50 values of three components (N,N dihydroxyethyl-p-toluidine, camphoroquinone, and N,N dimethylaminoethyl methacrylate) were above 100 mumol/L. The concentrations to which the cells and tissues are exposed in vivo are not known. This study should help to identify the concentrations of organic composite components which pose clinical cytotoxic hazards.
authors.affiliation: Department of Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor 48109-1078.
authors.forename: C T
authors.initials: CT
authors.lastname: Hanks
authors.forename: S E
authors.initials: SE
authors.lastname: Strawn
authors.forename: J C
authors.initials: JC
authors.lastname: Wataha
authors.forename: R G
authors.initials: RG
authors.lastname: Craig
mesh_terms: D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate; D002462:Cell Membrane / Q000187:drug effects; D003188:Composite Resins / Q000737:chemistry / Q000633:toxicity*; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects*; D008689:Methacrylates / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C; D011500:Protein Synthesis Inhibitors
chemical_list: D003188:Composite Resins; D008689:Methacrylates; D011500:Protein Synthesis Inhibitors; D017438:Bisphenol A-Glycidyl Methacrylate; D004247:DNA