Isolation and preliminary characterization of Escherichia coli mutants resistant to lethal action of the bacteriophage lambda P gene.
Both spontaneous and NTG-induced mutants of Escherichia coli 594 insensitive to the lethal action of lambda P gene were isolated and called rpl (resistant to P lethality). These mutants were of two types, showing different phenotypes. On type I rpl mutants, lambda cl- and lambda v1v3 did not plate, while lambda vir, lambda cl- c17, lambda imm434, and lambda imm21 did; plasmid pMR45 carrying the lambda P gene could not complement lambda imm21P- phage in type I mutants. On the other hand, the type II rpl mutants support the growth of all the above phages including lambda cl-. Neither type of rpl mutation affects growth of the bacteria.
Maiti
S
S
Department of Biochemistry, Bose Institute, Calcutta, India.
Das
B
B
Mandal
N C
NC
D010582:Bacteriophage lambda / Q000235:genetics* / Q000472:pathogenicity; D004926:Escherichia coli / Q000235:genetics*; D005804:Genes, Lethal; D005814:Genes, Viral; D015678:Viral Structural Proteins / Q000235:genetics*; D014779:Virus Replication
D015678:Viral Structural Proteins