[Monoclonal antibodies and T-cell clones against HLA antigens: production and characterization].
The production and characterization of 21 monoclonal antibodies (MoAbs) resulting from the immunization of Balb/c mice with human lymphoblastoid cell lines is reported. Twelve MoAbs seem to be detecting an HLA polymorphic determinant. Four MoAbs are recognizing monomorphic Class I molecules and another MoAb detects HLA Class II molecules. These results were confirmed by ELISA, cytotoxicity, immunofluorescence and immunoprecipitation. Class and subclass of the MoAbs were assessed by Ouchterlony analysis. Twenty two T cell clones were additionally generated by limiting dilution of lymphocytes stimulated by allogeneic peripheral blood lymphocytes on mixed lymphocyte cultures. Five of these clones were characterized, testing each one for its ability to proliferate in response to a panel of lymphoblastoid cell lines. It was found that they detect the HLA-A9, A23, A29-31, Bw62 and DR7 specificities respectively. These results indicate that the generation of cellular monoclonal reagents is apparently more productive than the generation of murine MoAbs specific for polymorphic variants of HLA antigens.
Domínguez
E
E
Instituto Venezolano de Investigaciones Científicas (I.V.I.C.), Centro de Medicina Experimental, Caracas, Venezuela.
Magli
M C
MC
Layrisse
Z
Z
Abad
M J
MJ
Quintero
M
M
Romano
E
E
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006680:HLA Antigens / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006680:HLA Antigens
Anticuerpos monoclonales y clones de linfocitos T contra antígenos HLA: producción y caracterización.