beta-Hexosaminidase expression in chick embryo fibroblasts in vitro.
1. Two forms of beta-hexosaminidase, similar to hexosaminidase A and hexosaminidase C, were separated by DEAE-cellulose chromatography in chick embryo skin fibroblasts in vitro. 2. beta-Hexosaminidase specific activity increases during development in cultured chick embryo skin fibroblasts in vitro. 3. Concanavalin-A treatment determines the increase of the neutral form, hexosaminidase C, during development. 4. Concanavalin-A reduces the specific activity of beta-hexosaminidase during development.
Beccari
T
T
Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università di Perugia, Italy.
Bodo
M
M
Becchetti
E
E
Pezzetti
F
F
Bellachioma
G
G
Orlacchio
A
A
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002848:Chromatography, DEAE-Cellulose; D003208:Concanavalin A / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D054818:Hexosaminidase A; D007700:Kinetics; D001619:beta-N-Acetylhexosaminidases / Q000096:biosynthesis*
D003208:Concanavalin A; D054818:Hexosaminidase A; D001619:beta-N-Acetylhexosaminidases