Cloning and sequence analysis of complementary DNA encoding a precursor for chicken natriuretic peptide.
Chicken alpha-natriuretic peptide (alpha-chNP) has been identified in chicken heart, which showed higher homology to brain natriuretic peptide (BNP) than to atrial natriuretic peptide (ANP). Complementary DNA (cDNA) clone encoding a chNP precursor (pre-chNP) was isolated from cardiac cDNA library and sequenced. Pre-chNP was 140-residue peptide carrying a 24-residue signal peptide at the N-terminus and alpha-chNP at the C-terminus, and did not exhibit high homology to porcine BNP except for the C-terminal region. However, a characteristic AT-rich nucleotide sequence commonly found in mammalian BNPs was also present in the 3'-untranslated region. Thus, chNP is concluded to be classified into the BNP-type.
Akizuki
N
N
Department of Biochemistry, Miyazaki Medical College, Japan.
Kangawa
K
K
Minamino
N
N
Matsuo
H
H
D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics*; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D006325:Heart Atria / Q000737:chemistry*; D006352:Heart Ventricles / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000235:genetics; D015345:Oligonucleotide Probes; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis*; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
D009419:Nerve Tissue Proteins; D015345:Oligonucleotide Probes; D011498:Protein Precursors; D012333:RNA, Messenger; D020097:Natriuretic Peptide, Brain; D009320:Atrial Natriuretic Factor; D004247:DNA