High recurrence of rearrangements involving chromosome 14 in an ataxia telangiectasia lymphoblastoid cell line and in its mutagen-treated derivatives.
The cytogenetic characterization of CH cell line obtained by Epstein-Barr-virus transformation of the lymphocytes of a patient affected by ataxia telangiectasia is reported. Control CH cells and 2 subcultures treated with the mutagens R7000 or NQO were developed in parallel and studied. A common chromosome anomaly, a der(14) t(11;14) (q13.2;q32), was found in all the studied karyotypes, indicating that it occurred either in vivo or early in vitro. In non-treated cultures, additional anomalies were present in 6 derived subclones. All R-7000 treated cells had the same karyotype corresponding to one of the subclones observed without prior treatment. All NQO-treated cells acquired 2 common anomalies, and could be differentiated into 2 subclones because of the addition of a t(7;14) or a t(11;14). Chromosome 14 was involved in various rearrangements after breakage in band q11.2 or q12 in 6/8 subclones. This was not correlated with tumorigenicity, which was clearly increased in mutagen-treated cells as tested by in vitro growth in semi-solid medium and in vivo by grafts into nude mice or growth on the chorio-allantoic membrane of chick embryos. The CH cell line and its derivatives appear to be a promising in vitro system, showing various stages progressing towards malignancy, and reproducing a number of chromosome anomalies spontaneously occurring in AT patients.
Lefrançois
D
D
Structure et Mutagenèse Chromosomiques, CNRS URA 620, Institut Curie, Paris, France.
Kokalj
N
N
Viegas-Péquignot
E
E
Montagnier
L
L
Dutrillaux
B
B
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000328:Adult; D000956:Antigens, Viral / Q000032:analysis; D001260:Ataxia Telangiectasia / Q000235:genetics*; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002869:Chromosome Aberrations; D002883:Chromosomes, Human, Pair 14; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007621:Karyotyping; D009153:Mutagens / Q000494:pharmacology; D009368:Neoplasm Transplantation; D009581:Nitrofurans / Q000494:pharmacology
D000956:Antigens, Viral; D009153:Mutagens; D009581:Nitrofurans; D015112:4-Nitroquinoline-1-oxide; C030911:2-nitro-7-methoxynaphtho(2-1b)furan