title: [Diagnostic possibilities of ANP blood measurements in dogs].
abstract: Atrial natriuretic peptide has been considered to be a major regulator in the body's water and salt homeostasis. Antagonizing those mechanisms leading to volume retention and overload (renin, angiotensin, aldosterone), ANP has been suggested to play a critical role in the pathology of certain diseases like renal failure, congestive heart failure or hypertension. In this regard, we measured ANP plasma concentration in normal healthy dogs and dogs with renal failure, congestive heart failure and Cushing syndrome. ANP levels were slightly decreased in dogs with Cushing disease (n = 9; 5.5 +/- 2 fmol/ml), increased in renal failure (n = 7; 16.2 +/- 5.8 fmol/ml, p less than 0.05) and markedly augmented in dogs with congestive heart failure (n = 14; 52.9 +/- 29.75 fmol/ml, p less than 0.01) as compared to healthy dogs (n = 6; 8.3 +/- 3.5 fmol/ml). Furthermore, characterization of the measured immunoreactivity (IR-ANP) revealed, that up to 50% of the IR-ANP in dogs with congestive heart failure corresponds to the ANP precursor molecule, not found in healthy subjects. This fact might present one possible explanation for the attenuated response to ANP in congestive heart failure. In addition, this finding may also serve a diagnostical purpose.
authors.affiliation: Institut für Pharmakologie, Toxikologie und Pharmazie der Tierärztlichen Fakultät Ludwig-Maximilians-Universität München.
authors.forename: A M
authors.initials: AM
authors.lastname: Vollmar
authors.forename: C
authors.initials: C
authors.lastname: Reusch
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D003480:Cushing Syndrome / Q000175:diagnosis / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D005260:Female; D006333:Heart Failure / Q000175:diagnosis / Q000662:veterinary*; D007676:Kidney Failure, Chronic / Q000175:diagnosis / Q000662:veterinary*; D008297:Male
chemical_list: D009320:Atrial Natriuretic Factor
vernacular_title: Diagnostische Möglichkeiten der ANP-Plasmamessung beim Hund.