Phosphatidylserine vesicles increase Ca2+ uptake by rat brain synaptosomes.
Phosphatidylserine (PS) vesicles incorporated into rat brain synaptosomes increased total Ca2+ uptake. Total Ca2+ uptake was resolved in three components: K+ depolarization-induced Ca2+ uptake, Na+/Ca2+ exchange, and passive Ca2+ entry, which were differently affected by PS depending on the amount of incorporated phospholipid. K+ depolarization-induced Ca2+ uptake was stimulated by 0.05-0.10 mumol PS/mg protein while 0.10-0.30 mumol PS/mg protein increased Na+/Ca2+ exchange activity and passive Ca2+ entry but not K+ depolarization-induced Ca2+ uptake. High amounts of incorporated PS also increased passive Rb+ uptake.
Floreani
M
M
Department of Pharmacology, University of Padova, Italy.
Debetto
P
P
Carpenedo
F
F
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002463:Cell Membrane Permeability; D010718:Phosphatidylserines / Q000494:pharmacology*; D051381:Rats; D019831:Sodium-Calcium Exchanger; D013574:Synaptosomes / Q000378:metabolism*
D015220:Calcium Channels; D002352:Carrier Proteins; D010718:Phosphatidylserines; D019831:Sodium-Calcium Exchanger; D002118:Calcium