title: Fusion properties of cells persistently infected with human parainfluenza virus type 3: participation of hemagglutinin-neuraminidase in membrane fusion.
abstract: Cells persistently infected with human parainfluenza virus type 3 (HPF3) exhibit a novel phenotype. They are completely resistant to fusion with each other but readily fuse with uninfected cells. We demonstrate that the inability of these cells to fuse with each other is due to a lack of cell surface neuraminic acid. Neuraminic acid is the receptor for the HPF3 hemagglutinin-neuraminidase (HN) glycoprotein, the molecule responsible for binding of the virus to cell surfaces. Uninfected CV-1 cells were treated with neuraminidase and then tested for their ability to fuse with the persistently infected (pi) cells. Neuraminidase treatment totally abolished cell fusion. To extend this result, we used a cell line deficient in sialic acid and demonstrated that these cells, like the neuraminidase-treated CV-1 cells, were unable to fuse with pi cells. We then tested whether mimicking the agglutinating function of the HN molecule with lectins would result in cell fusion. We added a panel of five lectins to the neuraminic acid-deficient cells and showed that binding of these cells to the pi cells did not result in fusion; the lectins could not substitute for interaction of neuraminic acid with the HN molecule in promoting membrane fusion. These results provide compelling evidence that the HN molecule of HPF3 and its interaction with neuraminic acid participate in membrane fusion and that cell fusion is mediated by an interaction more complex than mere juxtaposition of the cell membranes.
authors.affiliation: Department of Pediatrics, Mount Sinai School of Medicine, New York, New York 10029-6574.
authors.forename: A
authors.initials: A
authors.lastname: Moscona
authors.forename: R W
authors.initials: RW
authors.lastname: Peluso
mesh_terms: D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects; D003588:Cytopathogenic Effect, Viral; D015707:HN Protein / Q000494:pharmacology*; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D008561:Membrane Fusion / Q000187:drug effects*; D009438:Neuraminic Acids / Q000494:pharmacology; D010224:Parainfluenza Virus 3, Human / Q000502:physiology*; D018184:Paramyxoviridae Infections / Q000473:pathology; D014760:Viral Fusion Proteins / Q000494:pharmacology*
chemical_list: D015707:HN Protein; D037102:Lectins; D009438:Neuraminic Acids; D014760:Viral Fusion Proteins