Brain regional specificity and time-course of changes in the NMDA receptor-ionophore complex during ethanol withdrawal.
Previous work, using membrane receptor binding techniques, demonstrated an increase in hippocampal MK-801 binding sites in mice after chronic ethanol ingestion. The current studies, using quantitative autoradiography, demonstrate that chronic ethanol ingestion also produces increases in MK-801 binding in cerebral cortex, striatum and thalamus, as well as in hippocampus. The persistence of changes in MK-801 binding paralleled the time-course for ethanol withdrawal seizure susceptibility. These results support the hypothesis that an increase in the number of NMDA receptor/channel complexes in hippocampus, and possibly other brain regions, plays a role in the generation or expression of ethanol withdrawal seizures.
Gulya
K
K
Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20852.
Grant
K A
KA
Valverius
P
P
Hoffman
P L
PL
Tabakoff
B
B
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000473:pathology; D001923:Brain Chemistry / Q000187:drug effects*; D016291:Dizocilpine Maleate / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D008297:Male; D008566:Membranes / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects*; D012640:Seizures / Q000503:physiopathology; D013375:Substance Withdrawal Syndrome / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors
D016194:Receptors, N-Methyl-D-Aspartate; D000431:Ethanol; D016291:Dizocilpine Maleate