title: The gene expressions of macrophage colony-stimulating factor (MCSF) and MCSF receptor in the human myometrium during pregnancy: regulation by sex steroid hormones.
abstract: We investigated the biological effect of sex-steroid hormones, secreted from the corpus luteum and placenta, on the induction of mRNA encoding macrophage colony-stimulating factor (MCSF) and c-fms proto-oncogene (MCSF receptor) in the human uterine myometrium. Poly(A)+RNA was extracted from the myometrium of pregnant and non-pregnant uterine myometrium and then Northern blot analysis was performed on poly(A)+RNA. The myometrium of non-pregnant women expressed neither mRNA of macrophage colony-stimulating factor (MCSF) nor any transcript related to the c-fms proto-oncogene. On the other hand the myometrium of pregnant women expressed MCSF mRNA (4.7 kb) and two kinds of transcript related to the c-fms proto-oncogene (3.9 and 1.3 kb). The mRNAs of both MCSF and c-fms proto-oncogene were induced in the uterine myometrium of non-pregnant women under pseudopregnant therapy of mestranol and norethindrone. These results indicate that sex steroid hormone secreted from the corpus luteum of pregnancy and/or placenta may be deeply involved in the hypertrophic change of uterus during pregnancy by inducing MCSF and MCSF receptor (c-fms proto-oncogene protein product) in the myometrium.
authors.affiliation: Center for Adult Diseases, Osaka, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Azuma
authors.forename: F
authors.initials: F
authors.lastname: Saji
authors.forename: T
authors.initials: T
authors.lastname: Kimura
authors.forename: Y
authors.initials: Y
authors.lastname: Tokugawa
authors.forename: M
authors.initials: M
authors.lastname: Takemura
authors.forename: M
authors.initials: M
authors.lastname: Miki
authors.forename: M
authors.initials: M
authors.lastname: Ono
authors.forename: O
authors.initials: O
authors.lastname: Tanizawa
mesh_terms: D003338:Corpus Luteum / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000235:genetics*; D009215:Myometrium / Q000187:drug effects / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics*; D000090063:Proto-Oncogene Mas; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics*
chemical_list: D012739:Gonadal Steroid Hormones; C000715470:MAS1 protein, human; D011257:Pregnancy Proteins; D000090063:Proto-Oncogene Mas; D016173:Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor