[Retinoblastoma-culture in vitro and establishment of cell line].
60 cases of retinoblastoma cells have been cultured in vitro in recent 6 years. 10 of them grew more than 3 months, and one continuous retinoblastoma cell line (SO-Rb50) has been established for more than 3 years. In addition to the degree of malignancy of the tumor cells, selection of tumor tissues, components of culture media, optimal selection of seeding, passage and densities of cells play important roles in the establishment of immortal retinoblastoma cell line.
Zheng
J
J
National Ophthalmological Laboratories, Ministry of Public Health, China.
Yi
Y
Y
D003470:Culture Media; D005134:Eye Neoplasms / Q000473:pathology*; D006801:Humans; D012175:Retinoblastoma / Q000473:pathology*; D014407:Tumor Cells, Cultured
D003470:Culture Media