A region immediately adjacent to the origin of replication of bovine papilloma virus type 1 interacts in vitro with the nuclear matrix.
We have investigated the interaction of the 69% transforming fragment of the Bovine Papilloma Virus type 1 (BPV1) with the nuclear matrix from 1361.5 cells (NIH-3T3 cells transformed by a BPV chimeric construct). In vitro studies performed with end-labelled DNA fragments and nuclear matrices prepared using a high-salt extraction procedure demonstrate the binding of a 672 bp fragment adjacent to the viral origin of replication and containing the plasmid maintenance sequence (PMS-1). This fragment can be cleaved into two pieces (393 and 279 bp), both interacting equally well with the nuclear matrix. This indicates that a least two regions of the 672 bp DNA fragment are involved in the interaction.
Adom
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Unité de Recherches sur les Communications Hormonales, INSERM U-33, Hopital du Kremlin Bicêtre, France.
Richard-Foy
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D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002678:Chimera; D004261:DNA Replication; D004279:DNA, Viral / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D007700:Kinetics; D015530:Nuclear Matrix / Q000502:physiology*; D010957:Plasmids; D014162:Transfection
D004279:DNA, Viral