High-affinity receptor for interferon-gamma (IFN-gamma), a ubiquitous protein occurring in different molecular forms on human cells: blood monocytes and eleven different cell lines have the same IFN-gamma receptor protein.
High-affinity receptors for human IFN-gamma were analyzed using 13 different cells, including blood monocytes. Scatchard analysis showed one high-affinity binding site for each cell. One cross-linked complex between IFN-gamma and the receptor was detected, although their apparent molecular masses were variable in different cells, as also confirmed in immunoblots of membrane extracts. Variations in molecular masses were abolished if N-linked glycosylation was absent. Stable tryptic fragments contained the intact binding site for IFN-gamma and antibody epitopes characteristic of the extracellular domain of the IFN-gamma receptor of Raji cells and were of different sizes only if glycosylated. In addition, Northern analysis showed the same mRNA encoding the high-affinity IFN-gamma receptor in each cell analyzed. Thus, all cells including blood monocytes express the same high-affinity IFN-gamma receptor protein. N-linked sugars may give structural stability to the IFN-gamma receptor and are unlikely to be directly involved in IFN-gamma binding.
van Loon
A P
AP
Central Research Units, Hoffmann-La Roche Ltd, Basel, Switzerland.
Ozmen
L
L
Fountoulakis
M
M
Kania
M
M
Haiker
M
M
Garotta
G
G
D000911:Antibodies, Monoclonal; D015152:Blotting, Northern; D002460:Cell Line; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D007700:Kinetics; D007938:Leukemia; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008223:Lymphoma; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D011971:Receptors, Immunologic / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017471:Receptors, Interferon; D011994:Recombinant Proteins
D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D007371:Interferon-gamma