A characteristic Mls-1a response precedes Mls-1a anergy in vivo.
T cells expressing V beta 6 variable gene segments of the T cell receptor undergo blast formation and divide in mice after injection of lymphoid cells bearing minor lymphocyte-stimulating (Mls)-1a gene products. This in vivo Mls-1a response resembles in vitro Mls-1a stimulation; it is dose dependent, not MHC-class II haplotype restricted, but requires expression of functional IE gene products. The in vivo Mls-1a response is followed by a complete and specific in vivo Mls-1a anergy and a partial in vitro Mls-1a anergy. The measurement of a Mls-1a response in vivo and of the establishment of in vivo anergy to it provides a convenient method to assay Mls-1a reactivity of T cells in vivo on a cell-by-cell basis in terms of cell surface phenotype, size, and mitotic activity.
Dannecker
G
G
New York Medical College, Valhalla 10595.
Mecheri
S
S
Staiano-Coico
L
L
Hoffmann
M K
MK
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D002453:Cell Cycle; D000949:Histocompatibility Antigens Class II / Q000502:physiology; D007108:Immune Tolerance; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D016747:Minor Lymphocyte Stimulatory Antigens; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D016747:Minor Lymphocyte Stimulatory Antigens; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta