title: [Monoclonal antibodies to cyclodextrin-glycosyltransferase from Bacillus macerans].
abstract: Cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) was isolated from the cell-free culture medium of Bacillus macerans strain BKMB-506. The purified enzyme was used to immunize BALB/c mice. Polyclonal antibodies (PAbs) and 15 hybridoma cells producing monoclonal antibodies (MAbs) against CGTase were obtained. The properties of MAbs were studied using ELISA and immunoblotting. A method for detecting small amounts of CGTase was developed. The interactions of PAbs and MAbs with CGTase of Bac. circulans var. alkalophilis were investigated.
authors.forename: E
authors.initials: E
authors.lastname: Arbatova
authors.forename: M
authors.initials: M
authors.lastname: Reében
authors.forename: A
authors.initials: A
authors.lastname: Kestner
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001407:Bacillus / Q000201:enzymology; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005964:Glucosyltransferases / Q000276:immunology*; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase
vernacular_title: Monoklonal'nye antitela k tsiklodekstrin-gliukanotransferaze Bacillus macerans.