title: Interaction of human platelets with laminin and identification of the 67 kDa laminin receptor on platelets.
abstract: A microtitre adhesion assay has been developed to define parameters affecting the adherence of washed platelets to laminin. Adherence was optimally supported by Mg2+ and was inhibited by Ca2+ and by anti-laminin Fab fragments, but significant adhesion (75-90% of control) was found both in heparinized plasma containing physiological levels of bivalent cations and in plasma anti-coagulated with EGTA. Adherence was unaffected by platelet activation with ADP but was decreased by 50% by treatment with alpha-thrombin (1 unit/ml, 5 min). Adherence was unaffected by monospecific polyclonal antibodies to glycoprotein (GP) Ib and GPIV, and was normal with platelets from two patients with Glanzmann's thrombasthaenia, indicating that GPIb, the GPIIb/IIIa complex and GPIV are not involved in platelet-laminin interaction. Affinity chromatography of Triton-solubilized membranes on laminin-Sepharose followed by elution with 0.2 M-glycine/HCl (pH 2.85) identified a major band with a molecular mass of 67 kDa in the reduced and of 53 kDa in the unreduced form. This protein gave a positive reaction on Western blotting with a monospecific polyclonal antibody raised against the high-affinity laminin receptor isolated from human breast carcinoma tissue. The adhesion of platelets to laminin was inhibited by two monoclonal IgM antibodies specific to the LR-1 domain of the 67 kDa receptor. The binding protein was surface-oriented, as shown by flow cytofluorimetry and by the fact that it could be iodinated in intact platelets, but it was not labelled by the periodate-borotritide procedure, suggesting that it did not contain terminal sialic acid. The laminin-derived peptides Tyr-Ile-Gly-Ser-Arg and Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg-NH2, which constitute a complementary binding domain in laminin for the 67 kDa receptor, themselves supported platelet adhesion, bound to the receptor and inhibited the adhesion of platelets to laminin. In addition, Fab fragments of anti-Tyr-Ile-Gly-Ser-Arg antibody inhibited platelet adhesion to laminin. These results demonstrate that the high-affinity 67 kDa laminin receptor previously identified in a range of normal and transformed cells and its complementary Tyr-Ile-Gly-Ser-Arg binding domain play an important role in the interaction of platelets with laminin.
authors.affiliation: Cell Biology Laboratory, American Red Cross, Rockville, MD 20855.
authors.forename: N N
authors.initials: NN
authors.lastname: Tandon
authors.forename: E A
authors.initials: EA
authors.lastname: Holland
authors.forename: U
authors.initials: U
authors.lastname: Kralisz
authors.forename: H K
authors.initials: HK
authors.lastname: Kleinman
authors.forename: F A
authors.initials: FA
authors.lastname: Robey
authors.forename: G A
authors.initials: GA
authors.lastname: Jamieson
mesh_terms: D000595:Amino Acid Sequence; D001792:Blood Platelets / Q000276:immunology*; D002846:Chromatography, Affinity; D005434:Flow Cytometry; D006801:Humans; D007700:Kinetics; D007797:Laminin / Q000097:blood* / Q000276:immunology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009842:Oligopeptides / Q000138:chemical synthesis / Q000494:pharmacology; D010973:Platelet Adhesiveness / Q000187:drug effects; D010980:Platelet Membrane Glycoproteins / Q000502:physiology; D011971:Receptors, Immunologic / Q000302:isolation & purification / Q000378:metabolism*; D017474:Receptors, Laminin
chemical_list: D007797:Laminin; D009842:Oligopeptides; D010980:Platelet Membrane Glycoproteins; D011971:Receptors, Immunologic; D017474:Receptors, Laminin