title: [Binding of SSB-protein from Ehrlich ascites carcinoma cells with DNA and polyribonucleotides].
abstract: Binding of SSB-protein from Ehrlich ascites tumor to ssDNA from M13 phage leads to its compactization. The structure of the complex at the protein/DNA ratios far from the saturation level looks like "beads-on the string". DNA that was fully saturated with protein forms collapsed globular structure. Binding of the protein to the dsDNA from phage lambda increases its flexibility and decreases the coil dimensions; no "beads-on the string" structure are seen. The protein possess slight destabilizing effect on hairpin helices of M13DNA. Competition studies demonstrate that the binding properties of protein with polyribonucleotide lattices and DNA's decrease in ranking as follows: poly(rG) greater than or equal to poly(rI) greater than or equal to ssDNA greater than dsDNA greater than poly(rA) congruent to approximately poly(rU). Thus SSB-protein from Ehrlich ascites tumor differs significantly from its presumed prokaryotic analogs.
authors.forename: V A
authors.initials: VA
authors.lastname: Tronov
authors.forename: V A
authors.initials: VA
authors.lastname: Zaĭtsev
authors.forename: D I
authors.initials: DI
authors.lastname: Chernyĭ
authors.forename: A N
authors.initials: AN
authors.lastname: Koterov
authors.forename: I V
authors.initials: IV
authors.lastname: Filippovich
mesh_terms: D000818:Animals; D010582:Bacteriophage lambda / Q000378:metabolism; D001435:Bacteriophages / Q000378:metabolism; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism* / Q000648:ultrastructure; D004279:DNA, Viral / Q000378:metabolism* / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009994:Osmolar Concentration
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004268:DNA-Binding Proteins
vernacular_title: Sviazyvanie SSB-belka iz kletok astsitnoĭ kartsinomy érlikha s DNK i poliribonukleotidami.