Cloning and expression of a new pregnancy-specific beta 1-glycoprotein member.
Human pregnancy-specific beta 1-glycoproteins (PSGs) are a group of closely related placental glycoproteins and members of the immunoglobulin superfamily. To study possible functional roles of PSG, three type 1 cDNA clones which encode PSG in a N-A1-A2-B2-C domain arrangement have been characterized in this laboratory. Now, we report the cloning of a type II cDNA (PSG9) which is 1512 bp and encodes a PSG of 332 amino acids in a N-A1-B2-C domain arrangement. In vitro expression experiments demonstrate that PSG9 encodes a 36 kDa nonglycosylated protein which is processed to a glycosylated protein of 46 kDa. Ribonuclease protection analyses show that PSG mRNAs that share sequence similarity with the PSG9 transcript are expressed in both human placenta and placental fibroblasts.
Plouzek
C A
CA
Human Genetics Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
Watanabe
S
S
Chou
J Y
JY
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics; D005260:Female; D015870:Gene Expression; D015723:Gene Library; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D010920:Placenta / Q000502:physiology; D011247:Pregnancy; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000235:genetics*; D014176:Protein Biosynthesis; D011817:Rabbits; D015183:Restriction Mapping; D012156:Reticulocytes / Q000378:metabolism; D014158:Transcription, Genetic
D011268:Pregnancy-Specific beta 1-Glycoproteins; D004247:DNA