title: Molecular genetic approaches to the cytoskeleton in Dictyostelium.
abstract: Recent advances in molecular genetic techniques are being applied in Dictyostelium to test and expand prevailing views on the functioning of the actin-based cytoskeleton. Current research involves the disruption, by homologous recombination, of genes encoding cytoskeletal elements. We suggest combining classical and molecular genetic approaches to supplement these investigations.
authors.affiliation: Department of Cell Biology, Stanford University, CA 94305.
authors.forename: B
authors.initials: B
authors.lastname: Patterson
authors.forename: K M
authors.initials: KM
authors.lastname: Ruppel
authors.forename: J A
authors.initials: JA
authors.lastname: Spudich
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000378:metabolism; D003599:Cytoskeleton / Q000648:ultrastructure*; D004023:Dictyostelium / Q000235:genetics* / Q000648:ultrastructure; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005821:Genetic Techniques; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins; D005656:Fungal Proteins; D008840:Microfilament Proteins