[Specific proteolysis by fibrinolysin-coagulase from Yersinia pestis of Yersinia pseudotuberculosis outer membrane proteins coded by the Ca(2+)-dependence plasmid].
The pesticinogenicity 9.5 kb plasmid from Yersinia pestis strain EV76 has been marked by the kanamycin phosphotransferase gene inserted into PstI site and designated pP3. The obtained plasmid pP3 determines the synthesis of 45 kd pesticin, alpha and beta-forms of fibrinolysin coagulase (37 and 35 kd) and the 29, 19 and 13 kd proteins in Escherichia coli mini cells. When transferred into Yersinia pseudotuberculosis strain 6933 the plasmid causes the proteolysis of outer membrane proteins. The 150 kd protein is reduced to 138 kd, the 48.5 kd protein is reduced to 45 kd. The proteins secreted into the cultural medium (51 and 38 kd) are also cleaved. The proteolysis of the 150 kd protein was found to occur at the stage of secretion via the inner membrane. The purified fibrinolysin coagulase from Escherichia coli strain JM83 harbouring the plasmid pP3 induces the proteolysis in vitro of the isolated membrane proteins from Yersinia pseudotuberculosis strain 6953 similar to the proteolysis registered in vivo.
Shmelev
V A
VA
Cherepanov
P A
PA
Nosova
L Iu
LIu
Karimova
G A
GA
Popov
S G
SG
Noskov
A N
AN
D001345:Autoradiography; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D002118:Calcium / Q000378:metabolism*; D003030:Coagulase / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005341:Fibrinolysin / Q000378:metabolism*; D005798:Genes, Bacterial; D006868:Hydrolysis; D009154:Mutation; D010957:Plasmids; D015010:Yersinia pestis / Q000201:enzymology* / Q000472:pathogenicity; D015011:Yersinia pseudotuberculosis / Q000201:enzymology* / Q000235:genetics
D001425:Bacterial Outer Membrane Proteins; D003030:Coagulase; D005341:Fibrinolysin; D002118:Calcium
Spetsificheskiĭ proteoliz fibrinolizinokoagulazoĭ Yersinia pestis belkov vneshneĭ membrany, kodiruemykh plazmidoĭ Ca(2+)-zavisimosti v Yersinia pseudotuberculosis.