Interdependence of polymorphonuclear neutrophils and macrophages stained for N-acetyl-beta-glucosaminidase in lavage effluents from toluene diisocyanate-exposed rat lungs.
Male Sprague-Dawley rats were exposed to toluene diisocyanate (TDI) concentrations between 0.082 and 1.087 ppm for 4 h, and pulmonary lavage was carried out 24 h after initiation of the exposure. Cells recovered from the lavage effluents of TDI-exposed rat lungs were identified and counted, then pulmonary macrophages (PMs) resulting from cytocentrifuged preparations were examined for N-acetyl-beta-glucosaminidase (NAG) cytochemical staining. Exposure to TDI led to a parallel and concentration-dependent increase in the number of polymorphonuclear neutrophils (PMNs) and the proportion of PMs stained for NAG, suggesting that the same primary event initiates the two cell responses.
Hesbert
A
A
Institut National de Recherche et de Sécurité, Vandoeuvre, France.
Ban
M
M
Bonnet
P
P
Simon
P
P
Bottin
M C
MC
Lemonnier
M
M
de Ceaurriz
J
J
D000118:Acetylglucosaminidase / Q000378:metabolism*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D006651:Histocytochemistry; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D008264:Macrophages / Q000201:enzymology*; D008297:Male; D009504:Neutrophils / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013180:Sprains and Strains; D014051:Toluene 2,4-Diisocyanate / Q000633:toxicity*
D014051:Toluene 2,4-Diisocyanate; D000118:Acetylglucosaminidase