Inhibitory effects of biochanin A on mouse lung tumor induced by benzo(a)pyrene.
Biochanin A, an isoflavone compound, is reported to have an inhibitory effect on benzo(a)pyrene [B(a)P] metabolism. We examined the modifying effect of biochanin A on in vivo carcinogenesis using a mouse lung tumor model. As carcinogens, a single subcutaneous injection of 0.5mg of B(a)P was given within 24 hours after birth. The test groups were injected with 0.125mg of biochanin A in 0.1ml DMSO by i.p. 3 times a week for 6 weeks after weaning. All mice were sacrificed at week 9 and the incidence and multiplicity of lung tumors were examined. Concomitant administration of biochanin A showed a significant inhibitory effect on the incidence of tumor-bearing mice (12.5%, P < 0.01), as well as the mean number of tumors (0.13, P < 0.001), compared with the group treated with B(a)P alone in which the incidence was 57.1% and the mean number was 1.0. These results suggest that biochanin A has inhibitory potential on the development of mouse lung tumor induced by B(a)P.
Lee
Y S
YS
Laboratory of Cancer Pathology, Korea Cancer Center Hospital, Seoul.
Seo
J S
JS
Chung
H T
HT
Jang
J J
JJ
D000818:Animals; D000831:Animals, Newborn; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001564:Benzo(a)pyrene; D001835:Body Weight / Q000187:drug effects; D005260:Female; D019833:Genistein; D015994:Incidence; D007529:Isoflavones / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D051379:Mice; D009378:Neoplasms, Multiple Primary / Q000139:chemically induced / Q000517:prevention & control; D016019:Survival Analysis
D016588:Anticarcinogenic Agents; D007529:Isoflavones; D001564:Benzo(a)pyrene; D019833:Genistein; C004541:biochanin A