title: Synthesis of the NS 2 nonstructural protein messenger RNA of influenza A viruses occurs in the absence of viral protein synthesis.
abstract: The NS 2 messenger RNA (mRNA) of several influenza A viruses was shown to be synthesized in primary transcription. Analysis of in vitro translation products of mRNAs from infected MDCK cells treated with cycloheximide indicated that the NS 2 mRNA in addition to the NS 1 mRNA was synthesized with PR/8, Udorn, and Aichi viruses. The findings indicated that the NS 1 mRNA of these viruses was able to be spliced into the NS 2 mRNA as a primary transcript without viral protein synthesis, although the extent of splicing varied among different virus strains.
authors.affiliation: Department of Virology, Jichi Medical School, Tochigi-ken, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Odagiri
authors.forename: K
authors.initials: K
authors.lastname: Tobita
authors.forename: M
authors.initials: M
authors.lastname: Tashiro
mesh_terms: D000818:Animals; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics*; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D009980:Influenza A virus / Q000378:metabolism*; D014176:Protein Biosynthesis; D012326:RNA Splicing; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic; D014758:Viral Core Proteins / Q000096:biosynthesis / Q000235:genetics*; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins / Q000096:biosynthesis*
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; D003513:Cycloheximide