Sequential enzymatic synthesis and biodistribution of radiolabelled inositol and inositol analogs.
The desired radiolabelled inositol (Ins) and inositol-1-phosphate (IP) were obtained from radiolabelled glucose by sequential enzyme reactions in a short time. Rapid separation and fractionation of enzymes and labelled products from the reaction mixture was achieved by HPLC with a gel-permeation chromatography column. Examination of the biodistribution of radiolabelled Ins, IP and their acetylated analogs suggested that intact Ins labelled with 11C would be more effective than 11C-labelled acetylated Ins as a brain diagnostic agent for positron emission tomographic studies of the metabolism of phosphatidylinositol and its role as a second messenger in the brain.
Sasaki
T
T
Department of Radiopharmacy, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Ogihara-Umeda
I
I
Kojima
S
S
Nishigori
H
H
D000107:Acetylation; D000818:Animals; D001921:Brain / Q000378:metabolism; D002250:Carbon Radioisotopes; D007294:Inositol / Q000096:biosynthesis / Q000493:pharmacokinetics*; D007295:Inositol Phosphates / Q000493:pharmacokinetics; D008297:Male; D051379:Mice; D014018:Tissue Distribution
D002250:Carbon Radioisotopes; D007295:Inositol Phosphates; D007294:Inositol