[Influence of site and method of implantation on growth and invasion of human lung adenocarcinoma in nude mice].
In the present study, the growth and invasion of human lung adenocarcinoma cells (LTEP-a-2) transplanted into different sites (s.c. and footpad) and using different methods (including inoculation of tumor cell suspension and tumor fragment) in 12 nude mice were examined. The animals were divided into two groups; namely, subcutaneous inoculation (6 nude mice) and footpad inoculation (6 nude mice). Two transplantation sites were selected in each mouse; i.e. the tumor cell suspension was implanted into one site, and the tumor fragment was implanted into the opposing site of the same mouse. In the subcutaneous inoculation group, tumor growth (100%) was accompanied by no invasive behavior (including suspension and fragment). In the footpad inoculation group, 3/6 cases of inoculation by suspension saw tumor growth with invasive behavior; but in the case of tumor fragment transplantation no tumor growth was found. One murine sarcoma was found in the tissues surrounding the human lung adenocarcinoma cells. The mechanism of formation of this murine sarcoma is discussed. Our results show that human tumor growth in nude mice is obviously influenced by the site of inoculation.
Xue
K
K
Institute of Basic Medical Science, Beijing.
D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation / Q000379:methods*