title: Analysis of polyunsaturated fatty acids in blood serum after fish oil administration.
abstract: Free and total fatty acids in the blood serum of patients with hyperlipoproteinemia have been analysed as their methyl esters by capillary gas chromatography using an FFAP column. In one-step reactions the free fatty acids in serum react with methanol-acetyl chloride (50:1, v/v) at 25 degrees C, the total fatty acids (free plus esterified) are transesterified with methanol-toluene-acetyl chloride (8:2:1, v/v) at 100 degrees C. The quantification of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) is based on an internal standard (13,16,19-docosatrienoic acid) and on calibration standards. Under normal diet the concentrations of EPA and DHA are as follows (mean +/- S.D., n = 27): free EPA, 0.2 +/- 0.1 mg/dl; free DHA, 0.6 +/- 0.2 mg/dl; total EPA, 3.6 +/- 2.1 mg/dl; total DHA 11.4 +/- 3.1 mg/dl. Under a fish oil intake of 9 g per day, free and total EPA concentrations rise by ca. five- to six-fold, and free and total DHA concentrations by ca. two-fold.
authors.affiliation: Medizinische Universitätsklinik, Tübingen, Germany.
authors.forename: H M
authors.initials: HM
authors.lastname: Liebich
authors.forename: C
authors.initials: C
authors.lastname: Wirth
authors.forename: B
authors.initials: B
authors.lastname: Jakober
mesh_terms: D002849:Chromatography, Gas; D004281:Docosahexaenoic Acids / Q000097:blood*; D015118:Eicosapentaenoic Acid / Q000097:blood*; D004952:Esters / Q000097:blood; D005395:Fish Oils / Q000008:administration & dosage*; D006801:Humans; D006951:Hyperlipoproteinemias / Q000097:blood; D010919:Placebos
chemical_list: D004952:Esters; D005395:Fish Oils; D010919:Placebos; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid