Pyrophosphate formation as the most efficient condensation reaction of activated nucleotides.
When an oligonucleotide primer pG10 is incubated with the nucleotide analogue 9-[3-hydroxy-2-(hydroxymethyl)prop-1-yl] guanine diphosphate (pGp, I) in the presence of poly(C), addition of the monomer occurs almost exclusively at the 5'-terminal phosphate rather than the 3'-terminal cis-glycol. The implications of this finding in the context of prebiotic condensation reactions are discussed.
Rodriguez
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Salk Institute for Biological Studies, San Diego, CA 92122.
Orgel
L E
LE
D002851:Chromatography, High Pressure Liquid; D011756:Diphosphates / Q000737:chemistry*; D006150:Guanine Nucleotides / Q000737:chemistry*; D011066:Poly C / Q000737:chemistry*; D013698:Templates, Genetic
D011756:Diphosphates; D006150:Guanine Nucleotides; D011066:Poly C