title: Early in vitro detection of interleukin-2-like activities by pretreatment with allogeneic blood lymphocytes in miniature swine.
abstract: In order to determine the means of monitoring the immunological status of allograft recipients in miniature swine, an assay was developed to measure interleukin (IL)-2 production in vitro by pretreatment of donor peripheral blood lymphocytes (PBL). Miniature swine were given 0 to 4 weekly intravenous transfusions of 5-10 X 10(7) donor PBL incompatible at major histocompatibility complex (MHC) and assayed in vitro for donor specific immune IL-2-like activities. The results are summarized as follows: (1) IL-2-like activity in 24 hr and 48 hr supernatants from mixed lymphocyte cultures (MLC) with MHC-incompatible PBL was detected without pretreatment. The 48 hr MLC supernatant exhibited a high IL-2-like activity compared with the 24 hr; (2) IL-2-like activity after only one transfusion with MHC-incompatible PBL was higher than that without pretreatment; (3) IL-2-like activity in 4 weekly transfusions was detectable slightly earlier than that without pretreatment or three transfusions with MHC-incompatible PBL.
authors.affiliation: Department of Veterinary Surgery, Faculty of Agriculture, Yamaguchi University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Taura
authors.forename: Y
authors.initials: Y
authors.lastname: Mullen
authors.forename: T
authors.initials: T
authors.lastname: Papoian
authors.forename: T
authors.initials: T
authors.lastname: Tsunoda
authors.forename: T
authors.initials: T
authors.lastname: Shiogama
mesh_terms: D000818:Animals; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D007376:Interleukin-2 / Q000032:analysis*; D007959:Lymphocyte Culture Test, Mixed; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000276:immunology*; D008285:Major Histocompatibility Complex; D008297:Male; D013552:Swine; D013556:Swine, Miniature; D014184:Transplantation, Homologous
chemical_list: D007376:Interleukin-2