title: Lymphocyte proliferation test as a diagnostic aid in chromium contact sensitivity.
abstract: We investigated the clinical applicability of the lymphocyte proliferation test (LPT) in chromium contact sensitivity. 6 out of 8 chromium-sensitive patients were positive in the LPT, whereas none of 8 non-chromium-sensitive controls responded in vitro to tri- or hexavalent chromium compounds. LPT thus appeared to offer an additional diagnostic tool in chromium sensitivity. We also studied cellular interactions in 4 of our chromium-sensitive patients. Sensitized T-lymphocytes could be activated to proliferate only in the presence of accessory cells, of which epidermal Langerhans cells (LC) appeared more efficient than blood adherent cells.
authors.affiliation: Department of Clinical Sciences, University of Tampere, Finland.
authors.forename: L
authors.initials: L
authors.lastname: Räsänen
authors.forename: H
authors.initials: H
authors.lastname: Sainio
authors.forename: M
authors.initials: M
authors.lastname: Lehto
authors.forename: T
authors.initials: T
authors.lastname: Reunala
mesh_terms: D000328:Adult; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002857:Chromium / Q000009:adverse effects* / Q000378:metabolism; D003877:Dermatitis, Contact / Q000175:diagnosis*; D009783:Dermatitis, Occupational / Q000175:diagnosis / Q000209:etiology; D005260:Female; D006229:Hand Dermatoses / Q000175:diagnosis; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D008875:Middle Aged; D010328:Patch Tests
chemical_list: D002857:Chromium