Biosynthesis and secretion of beta-thromboglobulin by a human megakaryoblastic cell line (MEG-01).
The de-novo synthesis and secretion of beta-thromboglobulin (BTG) by a human megakaryoblastic cell line (MEG-01) were studied by measuring and immunoblotting of BTG in culture supernatant and immunoprecipitation of radiolabeled BTG synthesized after incubation with [35S]methionine. It was demonstrated that BTG synthesized by MEG-01 was secreted into culture media in a monomer form having a molecular weight of 8,800. Furthermore, we purified BTG from culture medium of MEG-01 with a heparin affinity column and compared BTG from MEG-01 with that from normal platelets. The molecular weights of BTG purified from both sources were 8,800 using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). These results provide direct evidence for the synthesis and secretion of BTG by megakaryocytes.
Tanabe
N
N
Nagoya National Hospital, Aichi, Japan.
Ogura
M
M
Hotta
T
T
Takamatsu
J
J
Tanaka
M
M
Saito
H
H
D001792:Blood Platelets / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007947:Leukemia, Megakaryoblastic, Acute / Q000473:pathology; D008533:Megakaryocytes / Q000378:metabolism*; D008970:Molecular Weight; D005810:Multigene Family; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism; D001620:beta-Thromboglobulin / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D003470:Culture Media; D009363:Neoplasm Proteins; D001620:beta-Thromboglobulin