Anaerobic energy provision in aged skeletal muscle during tetanic stimulation.
The effect of age on skeletal muscle anaerobic energy metabolism was investigated in adult (11 mo) and aged (25 mo) Fischer 344 rats. Hindlimb skeletal muscles innervated by the sciatic nerve were stimulated to contract with trains of supramaximal impulses (100 ms, 80 Hz) at a train rate of 1 Hz for 60 s, with an occluded circulation. Soleus, plantaris, and red and white gastrocnemius (WG) were sampled from control and stimulated limbs. All muscle masses were reduced with age (9-13%). Peak isometric tensions, normalized per gram of wet muscle, were lower throughout the stimulation in the aged animals (28%). The potential for anaerobic ATP provision was unaltered with age in all muscles, because resting high-energy phosphates and glycogen contents were similar to adult values. Anaerobic ATP provision during stimulation was unaltered by aging in soleus, plantaris, and red gastrocnemius muscles. In the WG, containing mainly fast glycolytic (FG) fibers, ATP and phosphocreatine contents were depleted less in aged muscle. In situ glycogenolysis and glycolysis were 90.0 +/- 4.8 and 69.3 +/- 2.6 mumol/g dry muscle (dm) in adult WG and reduced to 62.3 +/- 6.9 and 51.5 +/- 5.5 mumol/g dm, respectively, in aged WG. Consequently, total anaerobic ATP provision was lower in aged WG (224.5 +/- 20.9 mumol/g dm) vs. adult (292.6 +/- 7.6 mumol/g dm) WG muscle. In summary, the decreased tetanic tension production in aged animals was associated with a decreased anaerobic energy production in FG fibers. Reduced high-energy phosphate use and a greater energy charge potential after stimulation suggested that the energy demand was reduced in aged FG fibers.(ABSTRACT TRUNCATED AT 250 WORDS)
Campbell
C B
CB
School of Human Biology, University of Guelph, Ontario, Canada.
Marsh
D R
DR
Spriet
L L
LL
D000255:Adenosine Triphosphate / Q000378:metabolism; D000375:Aging / Q000502:physiology*; D000693:Anaerobiosis; D000818:Animals; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004558:Electric Stimulation; D004734:Energy Metabolism; D006019:Glycolysis; D008297:Male; D009119:Muscle Contraction / Q000502:physiology; D009132:Muscles / Q000502:physiology*; D010725:Phosphocreatine / Q000378:metabolism; D010732:Phosphofructokinase-1 / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344
D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D002950:Citrate (si)-Synthase; D010732:Phosphofructokinase-1