title: Binding of viral glycoprotein with trypsin and its relation to virulency. II. Comparison between bovine and Streptomyces griseus trypsins.
abstract: The two step nature of the binding reaction between trypsin and viral glycoproteins was further investigated using two types of trypsin, bovine trypsin and Streptomyces griseus trypsin. The experimental results were explained by the van der Waals energy operating in the second step, suggesting that the conformational aspects, in addition to the electrostatic nature, of the interacting peptides are decisive in this specific process.
authors.affiliation: Central Research Institute, Ishihara Sangyo Kaisha Ltd., Shiga, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Miyata
authors.forename: Y
authors.initials: Y
authors.lastname: Kiho
authors.forename: Y
authors.initials: Y
authors.lastname: Hosaka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002460:Cell Line; D009980:Influenza A virus / Q000378:metabolism* / Q000472:pathogenicity; D007700:Kinetics; D046911:Macromolecular Substances; D008432:Mathematical Computing; D008969:Molecular Sequence Data; D010222:Parainfluenza Virus 1, Human / Q000378:metabolism* / Q000472:pathogenicity; D011487:Protein Conformation; D013305:Streptomyces griseus / Q000201:enzymology; D014357:Trypsin / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000378:metabolism*; D010948:Viral Plaque Assay; D014774:Virulence; D014775:Virus Activation / Q000187:drug effects
chemical_list: D046911:Macromolecular Substances; D014760:Viral Fusion Proteins; D014357:Trypsin