[Regulation of the biosynthesis of hyaluronic acid. Role in the proliferation of fibroblasts].
The treatment of human skin fibroblasts with hyaluronidase stimulated the activity of hyaluronate synthase and the amount of hyaluronate secreted into the medium increased with the concentration of the enzyme and the time of the treatment. The maximal increase (about 3 fold) was independent of the type of glycosidic linkage cleaved, was inhibited neither by hyaluronate nor by oligosaccharides from hyaluronate and decreased in the late passage cultures. The increased hyaluronate synthesis was parallelled by 40% stimulation of the proliferation of fibroblasts up to 24th cell passage. The 10% stimulation of cell growth in late (36th passage) indicates a decrease in the ability of fibroblasts to respond to the degradation of the pericellular hyaluronate with in vitro ageing.
Moczar
M
M
Laboratoire de Biologie du Tissu Conjonctif, CNRS URA 1460, Faculté de Médecine, Paris.
D000328:Adult; D000375:Aging / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D012867:Skin / Q000166:cytology
D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase
Régulation de la biosynthèse de l'acide hyaluronique. Rôle dans la prolifération des fibroblastes.