Characterization of glucose-6-phosphate dehydrogenase variants in the Sudan--including GdKhartoum, a hyperactive slow variant.
Erythrocyte glucose-6-phosphate dehydrogenase (G6PD) was characterized in blood samples of 94 male subjects in Sudan having deficient and non-deficient electrophoretic variants. They comprised 44 GdB, 17 GdA, 19 GdB-, 11 GdA- and 3 nondeficient (GdKhartoum) variants. Biochemical characteristics including enzyme activity, electrophoretic mobility, Km for glucose-6-phosphate (G6P) and nicotinamide adenine dinucleotide phosphate (NADP), heat stability and pH optimum of all the common and deficient variants were consistent with the reported characteristics of these variants. The GdKhartoum variant had 90% mobility in TEB buffer and 100% in phosphate buffer, 120% activity, Km of 130 +/- 49 microns for G6P and 0.8 +/- 0.2 microns for NADP, lowered thermostability and an optimum pH of 7.6. This variant was not inhibited by 15 mM maleic acid, 10 mM iodoacetate and dehydro-iso-androsterone. All other variants were inhibited by dehydro-iso-androsterone but uninhibited by maleic acid and iodoacetate.
Saha
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Department of Physiology, Faculty of Medicine, University of Khartoum, Sudan.
Samuel
A P
AP
D000483:Alleles; D003687:Dehydroepiandrosterone / Q000494:pharmacology; D004592:Electrophoresis, Starch Gel; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology*; D014644:Genetic Variation / Q000187:drug effects; D006153:Guanosine Diphosphate / Q000235:genetics*; D006801:Humans; D007461:Iodoacetates / Q000494:pharmacology; D019807:Iodoacetic Acid; D008297:Male; D008298:Maleates / Q000494:pharmacology; D013397:Sudan; D014325:Tromethamine; D014944:World Health Organization
D007461:Iodoacetates; D008298:Maleates; D014325:Tromethamine; D006153:Guanosine Diphosphate; D003687:Dehydroepiandrosterone; C030272:maleic acid; D019807:Iodoacetic Acid