Detection of HSV-1 DNA in patients with Behçet's syndrome and in patients with recurrent oral ulcers by the polymerase chain reaction.
The polymerase chain reaction was used to detect HSV-1 DNA in genomic DNA extracted from peripheral blood leucocytes, in patients with Behçet's syndrome (BS), patients with recurrent oral ulcers and normal healthy controls. A 211-bp HSV-1 DNA fragment was found in a significant number of patients with BS (p less than 0.02). Serum anti-HSV-1 antibodies were also found in a higher proportion of patients with BS (p less than 0.02) than in healthy controls. However, virus-specific DNA was not detected in biopsy samples taken from oral ulcers in patients with BS.
Studd
M
M
Department of Immunology, United Medical School Guy's Hospital, London.
McCance
D J
DJ
Lehner
T
T
D000907:Antibodies, Bacterial / Q000032:analysis; D001528:Behcet Syndrome / Q000150:complications / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D016133:Polymerase Chain Reaction; D012008:Recurrence; D018139:Simplexvirus / Q000235:genetics* / Q000276:immunology; D014456:Ulcer / Q000150:complications / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D004279:DNA, Viral; D007074:Immunoglobulin G