The role of antibodies in the regulation of delayed-type hypersensitivity.
The contribution of B cells and antibodies to the regulation of delayed-type hypersensitivity (DTH) was investigated in mice rendered B-cell-deficient by treatment with anti-mu antibodies. In normal rabbit immunoglobulin (Ig)-treated mice as well as normal mice, the intravenous injection of a large amount of keyhole limpet haemocyanin (KLH) suppressed DTH, and serum titres of the anti-KLH antibody were significantly elevated. However, in anti-mu-treated mice, the intravenous injection of a large amount of KLH could not induce either suppression of DTH or the elevation of anti-KLH antibody titres. The transfer of anti-KLH antibodies suppressed DTH in a H-2 non-restricted, probably Igh-restricted, way in anti-mu-treated mice. In addition, the transfer of anti-KLH antibodies induced effector-phase suppressor T cells whose phenotype was L3T4-, Lyt-2+. We concluded that antibodies play a significant role in the regulation of DTH.
Morikawa
Y
Y
Department of Pathology, Wakayama Medical School, Japan.
Kuribayashi
K
K
Yoshikawa
F
F
Fujita
K
K
Mizushima
A
A
Kakudo
K
K
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006433:Hemocyanins / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007165:Immunosuppression Therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000906:Antibodies; D000941:Antigens; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin