title: Coexpression of the genes for platelet-derived growth factor B-chain receptor and macrophage colony-stimulating factor 1 receptor during monocytic differentiation.
abstract: Receptors for platelet-derived growth factor (PDGF) have not been identified previously to our knowledge in human myeloid cells that also produce PDGF. Here we report that phorbol ester-treated myeloid cells differentiated along the monocytic lineage express both a full-length 5.5-kilobase (kb) mRNA and a predominant, truncated 4.6-kb mRNA coding for the PDGF B-chain receptor (PDGF-BR). PDGF-BR was identified in phorbol ester-differentiated myeloid cells by indirect immunofluorescence with an antibody specific to PDGF-BR. This anti-PDGF-BR was also used in immunoprecipitation studies to demonstrate that lysates of phorbol ester-differentiated myeloid cells contain PDGF-BR molecules of 37 kDa to 130 kDa. The results also show that the tandemly linked genes for PDGF-BR and the macrophage colony-stimulating factor 1 receptor are coexpressed in the phorbol ester-differentiated myeloid cells. Expression of these two receptor genes has not been shown previously in any cell type to our knowledge.
authors.affiliation: Stehlin Foundation for Cancer Research, St. Joseph Hospital Cancer Research Laboratory, Houston, TX 77003.
authors.forename: P
authors.initials: P
authors.lastname: Pantazis
authors.forename: S
authors.initials: S
authors.lastname: Kharbanda
authors.forename: A S
authors.initials: AS
authors.lastname: Goustin
authors.forename: T
authors.initials: T
authors.lastname: Galanopoulos
authors.forename: D
authors.initials: D
authors.lastname: Kufe
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006801:Humans; D046911:Macromolecular Substances; D009000:Monocytes / Q000166:cytology / Q000502:physiology; D010982:Platelet-Derived Growth Factor / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000032:analysis / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000032:analysis / Q000235:genetics*; D017479:Receptors, Platelet-Derived Growth Factor
chemical_list: D046911:Macromolecular Substances; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D016186:Receptor, Macrophage Colony-Stimulating Factor; D017479:Receptors, Platelet-Derived Growth Factor