[Effects of diphenoxylate hydrochloride and dl-15 methyl prostaglandin F2 alpha methyl ester on rat luteal cells].
Diphenoxylate hydrochloride (R1132) at concentrations of 10 and 20 micrograms/ml or dl-15 methyl-PGF2 alpha methyl ester (PG05) at levels of 5 and 10 micrograms/ml was shown to have no effect on progesterone secretion by luteal cells in vitro in the absence of hCG. A marked increase in progesterone level was elicited by hCG as high as 3-8 fold the original value. The steroidogenic response of luteal cells to hCG was inhibited by R1132 and PG05. R1132 at a daily dose of 10 mg/kg or PG05 at a daily dose of 0.1 mg/kg for 5 days showed no obvious effect on ovary progesterone secretion in pseudopregnant rat. However, treatment with R1132 10 mg/kg plus PG05 0.1 mg/kg resulted in a decrease in the progesterone production of ovary in vitro. R1132 and PG05 at doses of 50 mg/kg and 0.5 mg/kg, respectively, exhibited an inhibitory effect on the adenylate cyclase activity.
Wang
N G
NG
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Guan
M Z
MZ
Zuo
X C
XC
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D015237:Dinoprost / Q000494:pharmacology*; D004157:Diphenoxylate / Q000494:pharmacology*; D005260:Female; D008184:Luteal Cells / Q000187:drug effects / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism*; D011555:Pseudopregnancy / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D011374:Progesterone; D004157:Diphenoxylate; D015237:Dinoprost; D000262:Adenylyl Cyclases