title: Mutant strains of Streptomyces cinnamonensis protoplasts. Cultural and physiological conditions.
abstract: Mycelium of Streptomyces cinnamonensis mutant strains cultivated in a synthetic medium with glycine produced protoplasts after lysis of cell walls with lysozyme. The protoplast yield was up to 95%. The protoplasts could revert and mycelial forms were thus regenerated. In a sucrose-containing medium the protoplasts stored at 4 degrees C were stable for 2 d.
authors.affiliation: Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
authors.forename: J
authors.initials: J
authors.lastname: Matĕjů
authors.forename: J
authors.initials: J
authors.lastname: Marsálková
authors.forename: M
authors.initials: M
authors.lastname: Nohýnek
authors.forename: N
authors.initials: N
authors.lastname: Steinerová
mesh_terms: D003470:Culture Media; D005998:Glycine; D009113:Muramidase; D009154:Mutation; D009172:Mycology / Q000379:methods; D011523:Protoplasts / Q000166:cytology / Q000502:physiology; D013302:Streptomyces / Q000166:cytology / Q000235:genetics* / Q000502:physiology
chemical_list: D003470:Culture Media; D009113:Muramidase; D005998:Glycine