title: Improved quantitation of 13-cis- and all-trans-acitretin in human plasma by normal-phase high-performance liquid chromatography.
abstract: A reliable analytical method has been developed for measurement of 13-cis- and all-trans-acitretin (Neotigason) in human plasma by normal-phase high-performance liquid chromatography, with ultraviolet detection. Human plasma was obtained after centrifugation of whole blood samples and deproteinized by ethanolic denaturation. After liquid-liquid extraction with water-n-hexane, and aliquot ws chromatographed on a silica column using isocratic elution with n-hexane-methylsalicylate-acetic acid (200:18:0.6, v/v). The wavelength was set at 360 nm, and for plasma samples a limit of quantification of 3-4 ng/ml was obtained. All manipulations were carried out under dim light conditions to prevent photoisomerization.
authors.affiliation: Laboratoria voor Medische Biochemie en voor Klinische Analyse, Faculteit Farmaceutische Wetenschappen, Rijksuniversiteit Gent, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Meyer
authors.forename: W E
authors.initials: WE
authors.lastname: Lambert
authors.forename: A P
authors.initials: AP
authors.lastname: De Leenheer
authors.forename: J P
authors.initials: JP
authors.lastname: Bersaques
authors.forename: A H
authors.initials: AH
authors.lastname: Kint
mesh_terms: D017255:Acitretin; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics
chemical_list: D014212:Tretinoin; D017255:Acitretin