Identification of a cell membrane protein that binds alveolar surfactant.
Alveolar surfactants are complex mixtures of proteins and phospholipids produced by type II alveolar cells and responsible for lowering pulmonary surface tension. The process by which surfactant is produced and exported and by which its production by pulmonary cells is regulated are not well understood. This study was designed to identify a cellular receptor for surfactant constituents. To do so, monoclonal anti-idiotypic antibodies directed against antibodies to porcine and rabbit surfactant proteins were prepared. These monoclonal anti-idiotypic antibodies bind both alveolar lining and bronchial epithelial cells in rabbit, porcine, and human lungs. Macrophages and other nonepithelial cells do not react with these antibodies. Western blot analysis indicates that both A2R and A2C recognize the same proteins in both pig and rabbit lungs: a 30-kd protein and additional proteins at 52 and 60 kd. Preincubating lung wash cells with A2C or A2R prevents binding of porcine or rabbit surfactant preparations, respectively, by these cells. Preincubating frozen sections of lung tissue with surfactant inhibits binding of A2R and A2C to the lung. Antibody directed to a cell membrane protein that recognizes alveolar surfactant may be useful in elucidating the structure and function of this receptor and in understanding the cellular physiology and pathophysiology of the surfactant system.
Strayer
D S
DS
Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, Houston 77030.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D005434:Flow Cytometry; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure*; D011663:Pulmonary Surfactants / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D013552:Swine
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; D011663:Pulmonary Surfactants; D011956:Receptors, Cell Surface