Are several G proteins involved in the different effects of endothelin-1 in mouse striatal astrocytes?
High-affinity specific receptors of endothelin (ET-1) were identified on primary cultures of mouse embryo striatal astrocytes by binding experiments performed with 125I-ET-1. Stimulation of production of inositol phosphates, a biphasic increase of the intracellular calcium concentration, and inhibition of cyclic AMP accumulation were observed in the same cells under ET-1 stimulation. Pretreatment of these cells with Bordetella pertussis toxin affected these effects to different extends, an observation suggesting that they are mediated by multiple transduction pathways, possibly involving several guanine nucleotide-binding proteins.
Marin
P
P
Chaire de Neuropharmacologie, INSERM U. 114, Collège de France, Paris.
Delumeau
J C
JC
Durieu-Trautmann
O
O
Le Nguyen
D
D
Prémont
J
J
Strosberg
A D
AD
Couraud
P O
PO
D000818:Animals; D001253:Astrocytes / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D003342:Corpus Striatum / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D000242:Cyclic AMP / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism; D016232:Endothelins / Q000378:metabolism / Q000494:pharmacology*; D019204:GTP-Binding Proteins / Q000502:physiology*; D007295:Inositol Phosphates / Q000378:metabolism; D051379:Mice / Q000196:embryology; D008815:Mice, Inbred Strains; D009994:Osmolar Concentration
D016232:Endothelins; D007295:Inositol Phosphates; D000242:Cyclic AMP; D019204:GTP-Binding Proteins; D002118:Calcium