title: Effects of vasopressin and cAMP on single amiloride-blockable Na channels.
abstract: To determine the mechanism by which vasopressin increases sodium transport in sodium-transporting, tight epithelia, we examined single amiloride-blockable Na channels in membrane patches from cultured distal nephron cells (A6) either before or after treatment with arginine vasopressin. Pretreatment of cells with vasopressin (40 mU/ml) for 40-50 min increases NPo (N, the number of Na channels; Po, the open probability of an individual Na channel). The increase in NPo is due to an increase in the number of conductive Na channels with little or no change in the open probability of individual Na channels. Pretreatment of cells for 1 h with 1 mM N6,2'-O-dibutyryladenosine 3', 5'-cyclic monophosphate (DBcAMP) also increased NPo. The increase in NPo caused by DBcAMP pretreatment is also due to the increase in the number of conductive Na channels with no change in the open probability of individual Na channels. Cells pretreated with cholera toxin (CTX; 250 ng/ml) for 4 h appeared similar to cells that had been treated with vasopressin or DBcAMP; that is, the number of Na channels per patch increased with little or no effect on the open probability of individual Na channels. For patches from many untreated cells, when the frequency of occurrence is plotted against the number of channels in an individual patch, the histogram consists of a single peak with a number of channels per patch of 2.0 +/- 1.5 (+/- SD, 126 patches). After pretreatment of cells with vasopressin, DBcAMP, or CTX, the same histogram contains two peaks after vasopressin of 1.8 +/- 1.2 and 9.2 +/- 1.5 (+/- SD, 38 and 53 patches, respectively). These observations suggest that pretreatment of cells with vasopressin, DBcAMP, or CTX may act by promoting insertion of clusters of new sodium channels.
authors.affiliation: Department of Physiology, Emory University School of Medicine, Atlanta, Georgia 30322.
authors.forename: Y
authors.initials: Y
authors.lastname: Marunaka
authors.forename: D C
authors.initials: DC
authors.lastname: Eaton
mesh_terms: D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D003994:Bucladesine / Q000494:pharmacology*; D002455:Cell Division; D002460:Cell Line; D002772:Cholera Toxin / Q000494:pharmacology; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods; D000242:Cyclic AMP / Q000502:physiology*; D015640:Ion Channel Gating; D008433:Mathematics; D008564:Membrane Potentials / Q000187:drug effects; D008954:Models, Biological; D009399:Nephrons; D015222:Sodium Channels / Q000187:drug effects / Q000502:physiology*
chemical_list: D015222:Sodium Channels; D001127:Arginine Vasopressin; D003994:Bucladesine; D000584:Amiloride; D002772:Cholera Toxin; D000242:Cyclic AMP