title: Enhancement of hepatic macrophages in septic rats and their inhibitory effect on hepatocyte function.
abstract: In the present study, the function of hepatic macrophages and the modulation of hepatocytes by sepsis-elicited hepatic macrophages were investigated in rats with induced sepsis. The functional state of hepatic macrophages was determined by the following indicators: phagocytic index, protein-synthesizing capacity, and superoxide (O2-) producing capacity. These indices of changes in hepatic macrophages were much higher in rats with sepsis than in healthy controls. Moreover, the activated hepatic macrophages had some biological properties which were different from those of the resident Kupffer cells. It was found that protein synthesis by cultured hepatocytes was inhibited in the co-culture system of hepatocytes and sepsis-elicited hepatic macrophages, and that the supernatant of hepatic macrophages from rats with sepsis also reduced the protein-synthesizing capacity of cultured hepatocytes. Thus, activated hepatic macrophages may play a role in inducing hepatic dysfunction in sepsis.
authors.affiliation: First Department of Surgery, Kyoto University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Monden
authors.forename: S
authors.initials: S
authors.lastname: Arii
authors.forename: S
authors.initials: S
authors.lastname: Itai
authors.forename: T
authors.initials: T
authors.lastname: Sasaoki
authors.forename: Y
authors.initials: Y
authors.lastname: Adachi
authors.forename: N
authors.initials: N
authors.lastname: Funaki
authors.forename: T
authors.initials: T
authors.lastname: Tobe
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D008070:Lipopolysaccharides / Q000494:pharmacology; D008099:Liver / Q000473:pathology* / Q000503:physiopathology; D008264:Macrophages / Q000502:physiology*; D008297:Male; D009895:Opsonin Proteins / Q000494:pharmacology; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D018805:Sepsis / Q000473:pathology* / Q000503:physiopathology; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
chemical_list: D008070:Lipopolysaccharides; D009895:Opsonin Proteins; D013481:Superoxides; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate