Preimplantation embryo biopsy: detection of trisomy in a single cell biopsied from a four-cell mouse embryo.
This paper describes an efficient technique for the production of metaphase spreads from single blastomeres biopsied from four-cell preimplantation mouse embryos. The karyotype obtained by chromosomal analysis of single biopsied cells is shown to be fully predictive of subsequent fetal karyotype. The data in this study also demonstrate that the entire process of embryo biopsy and karyotypic analysis of biopsied blastomeres does not adversely affect the ability of biopsied embryos to form fetuses after transfer into pseudopregnant recipients. This study has potential clinical relevance in that it demonstrates that chromosomally defective embryos can be accurately identified before implantation. In addition, the techniques developed in this study may facilitate more efficient procedures for the genesis of animal models for human disorders such as Down syndrome and Alzheimers disease.
Kola
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I
Centre for Early Human Development, Monash University, Clayton, Victoria, Australia.
Wilton
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L
D000818:Animals; D001706:Biopsy / Q000379:methods*; D001757:Blastomeres / Q000166:cytology*; D002478:Cells, Cultured; D004314:Down Syndrome / Q000175:diagnosis; D047108:Embryonic Development; D005314:Embryonic and Fetal Development; D005260:Female; D007621:Karyotyping; D008297:Male; D008677:Metaphase; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008846:Micromanipulation / Q000379:methods; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D014314:Trisomy