Early region 4 sequence and biological comparison of two isolates of mouse adenovirus type 1.
The DNA sequence of 88-100 map units of mouse adenovirus type 1 (MAV-1) was determined. One translational open reading frame showed 48% sequence similarity to a human adenovirus type 2 early region 4 protein. Based on the protein similarity, genome location, and transcriptional polarity, we concluded that this region of MAV-1 corresponds to early region 4. A 241-bp sequence consisting of 10 imperfect direct repeats with sequence similarity to minisatellite DNA was found in this region. Two virus isolates with different passage histories were examined and were found to have a sequence polymorphism within this region. The two viruses were compared for growth in cell culture and mice and small quantitative differences were observed only in vivo.
Ball
A O
AO
Department of Genetics, University of Georgia, Athens 30602.
Beard
C W
CW
Villegas
P
P
Spindler
K R
KR
D000256:Adenoviridae / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D000257:Adenoviridae Infections / Q000382:microbiology / Q000401:mortality; D000260:Adenoviruses, Human / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004279:DNA, Viral; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014776:Virus Cultivation
D004279:DNA, Viral