Nucleotide sequence and genomic organization of Aedes densonucleosis virus.
Over 99% of the genome of Aedes densonucleosis virus was determined. Two types of the viral DNA were found that differ only in four nucleotides (nt) in the 5' noncoding part and whose sizes are 4009 nt (more copious) and 4012 nt, respectively. Both 146 nt at the 3' end and 164 nt at the 5' end could assume a similar T-shaped structure; but unlike the adeno-associated virus, Aedes DNA has a unique primary DNA sequence at each terminus. However, the crossarms of these structures are built of the same sequences. An imperfect direct repeat of 34 nt was observed in the 5' noncoding part. The plus strand has three large open reading frames (ORF): a left ORF, a right ORF, and a mid ORF (within the left ORF). The left ORF codes for the nonstructural protein NS-1 (97.5K) featured by an NTP-binding domain, and the right ORF encodes the both capsid proteins, the smaller of which (39K) is supposed to be derived from the larger one (40.5K) by proteolytic cleavage. There is also an ORF in the minus strand. The putative polypeptide coded by this ORF is extremely hydrophobic.
Afanasiev
B N
BN
Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Galyov
E E
EE
Buchatsky
L P
LP
Kozlov
Y V
YV
D000330:Aedes / Q000382:microbiology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010321:Parvoviridae / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014758:Viral Core Proteins / Q000235:genetics*; D017361:Viral Nonstructural Proteins
D003062:Codon; D004279:DNA, Viral; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins