Inclusion body myositis and paramyxoviruses.
Inclusion body myositis (IBM) is a distinct type of muscle disease. The characteristic electron microscopic findings, intranuclear or intracytoplasmic inclusions composed of microtubular filaments, morphologically resemble paramyxovirus nucleocapsids. These findings and the reported immunoreactivity of the inclusions with mumps virus antibodies have suggested that inclusion body myositis is a chronic virus infection. We analyzed skeletal muscle specimens from three patients with characteristic light microscopic features and electron microscopically verified inclusions of IBM by immunocytochemistry using antibodies raised against members of the paramyxovirus group, and by in situ hybridization with a cRNA probe representing the mumps virus nucleocapsid gene. The specificity of the reactions was demonstrated with infected and uninfected cultured cells. No immunocytochemical staining or hybridization signal was observed in biopsy specimens from IBM patients. These findings speak against a paramyxovirus etiology of IBM.
Kallajoki
M
M
Department of Pathology, University of Turku, Finland.
Hyypiä
T
T
Halonen
P
P
Orvell
C
C
Rima
B K
BK
Kalimo
H
H
D000368:Aged; D000914:Antibodies, Viral / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D002479:Inclusion Bodies / Q000648:ultrastructure*; D008297:Male; D008459:Measles virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D008875:Middle Aged; D009109:Mumps virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D009132:Muscles / Q000648:ultrastructure*; D009220:Myositis / Q000382:microbiology* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D010252:Paramyxoviridae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D004279:DNA, Viral