Immunomodulatory effect of cimetidine on the proliferative responses of splenocytes from T. cruzi-infected rats.
The immunomodulatory effect of cimetidine (CIM), a histamine type-2 receptor antagonist, was evaluated in respect to the blastogenic response to Con A of Wistar Furth (WF) rats infected by the Y strain of Trypanosoma cruzi (T. cruzi). Enhancement of blastogenesis of normal splenocytes was observed at a concentration of 10(3) M. However, the splenocytes from infected animals responded to concentrations of CIM ranging from 10(-8) to 10(-3) M. The mitogenic response to Con A of cells from infected animals was restored in the presence of CIM. The results show that CIM modulates the "in vitro" proliferative response of cells from T. cruzi-infected rats and suggest an immunoregulatory role of histamine and/or of cells that express H2 receptors in this infection.
Sato
M N
MN
Laboratório de Imunogenética e Transplante Experimental (LIM 73), Faculdade de Medicina, Universidade de São Paulo, Brazil.
Yamashiro-Kanashiro
E H
EH
Tanji
M M
MM
Kaneno
R
R
Iqueoka
R Y
RY
Duarte
A J
AJ
D000818:Animals; D014355:Chagas Disease / Q000276:immunology*; D002927:Cimetidine / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051381:Rats; D011920:Rats, Inbred WF; D011970:Receptors, Histamine H2 / Q000187:drug effects / Q000276:immunology; D013154:Spleen / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology
D011970:Receptors, Histamine H2; D003208:Concanavalin A; D002927:Cimetidine