Viability of Ascaris lumbricoides eggs eliminated after anti-helminthic therapy.
The viability of Ascaris lumbricoides eggs passed in the feces was evaluated after treatment of patients with one of the anti-helminthic drugs (thiabendazole, levamisole, cambendazole, pyrantel pamoate, mebendazole or praziquantel). For each drug, a group of 5 children was selected and their feces collected 24 h before treatment and 24, 48 and 72 h after drug administration, except for mebendazole, with the feces being collected throughout the period of treatment. After sedimentation, the total amount of eggs from each collection was transferred to tissue culture flasks containing 10 ml H2SO4 0,1N, with the addition of 3 drops of a miconazole solution, and incubated at 28 degrees C, individually, for 80 days. The flasks were maintained open and the culture were oxygenated daily by manual agitation. On the 80th day of culture, 20-days-old albino mice were inoculated with 3,200 embryonated eggs, per os. Larvae were recovered from their lungs and hearts, on the 8th day after infection, according to Baerman's method (Morais, 1948). Thiabendazole showed 100.0% ovicidal capacity as early as 48 h after treatment. Inhibition of embryonal development was observed when thiabendazole was used. This drug also had an effect on the eggs infectivity when inoculated into normal mice. No significant effect on embryonal development was observed for the other drugs tested.
Massara
C L
CL
Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, MG, Brasil.
Costa
H M
HM
De Souza
D W
DW
Souza
M S
MS
Carvalho
O dos S
Odos S
D000293:Adolescent; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology / Q000627:therapeutic use*; D001196:Ascariasis / Q000188:drug therapy*; D001200:Ascaris / Q000187:drug effects / Q000502:physiology; D002648:Child; D004531:Eggs; D006801:Humans; D051379:Mice; D010270:Parasite Egg Count
D000871:Anthelmintics