Induction of serotonin release from mast cells by lymphocyte activators is dependent upon implantation of lymphocyte plasma membrane components.
Plasma membranes (PM) isolated from mouse splenic lymphocytes were successfully fused to rat basophilic leukemia (RBL) cells using intact Sendai virus virions (SV). A two-step procedure was used in which SV were first fused with the PM to create PM + SV vesicles; the vesicles formed were then incubated with the RBL cells. Insertion of lymphocyte PM into the RBL cell's membrane endows a high rate of serotonin secretion upon stimulation of the implanted RBL cells with antimouse Ig antibodies or with concanavalin-A. The results of the present work clearly suggest that activation signals can be delivered via implantation of foreign membrane preparation containing specific receptors, thus rendering the target cells susceptible to stimulation by specific reagents.
Abramovitz
B
B
Department of Human Microbiology, Sackler Faculty of Medicine, Tel Aviv University, Israel.
Altboum
I
I
Lapidot
M
M
Loyter
A
A
Zan-Bar
I
I
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D006801:Humans; D007700:Kinetics; D015471:Leukemia, Basophilic, Acute; D007942:Leukemia, Experimental; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000502:physiology*; D008407:Mast Cells / Q000502:physiology*; D008561:Membrane Fusion; D051379:Mice; D008807:Mice, Inbred BALB C; D010222:Parainfluenza Virus 1, Human / Q000502:physiology; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D014771:Virion / Q000502:physiology
D012701:Serotonin