A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types.
cDNA clones corresponding to an Mr approximately 80,000 receptor (type I receptor) for interleukin-1 (IL-1) have been isolated previously by mammalian expression. Here, we report the use of an improved expression cloning method to isolate human and murine cDNA clones encoding a second type (Mr approximately 60,000) of IL-1 receptor (type II receptor). The mature type II IL-1 receptor consists of (i) a ligand binding portion comprised of three immunoglobulin-like domains; (ii) a single transmembrane region; and (iii) a short cytoplasmic domain of 29 amino acids. This last contrasts with the approximately 215 amino acid cytoplasmic domain of the type I receptor, and suggests that the two IL-1 receptors may interact with different signal transduction pathways. The type II receptor is expressed in a number of different tissues, including both B and T lymphocytes, and can be induced in several cell types by treatment with phorbol ester. Both IL-1 receptors appear to be well conserved in evolution, and map to the same chromosomal location. Like the type I receptor, the human type II IL-1 receptor can bind all three forms of IL-1 (IL-1 alpha, IL-1 beta and IL-1ra). Vaccinia virus contains an open reading frame bearing strong resemblance to the type II IL-1 receptor.
McMahan
C J
CJ
Department of Molecular Biology, Immunex Research and Development Corporation, Seattle, WA 98101.
Slack
J L
JL
Mosley
B
B
Cosman
D
D
Lupton
S D
SD
Brunton
L L
LL
Grubin
C E
CE
Wignall
J M
JM
Jenkins
N A
NA
Brannan
C I
CI
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D001483:Base Sequence; D005075:Biological Evolution; D015152:Blotting, Northern; D015139:Blotting, Southern; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002874:Chromosome Mapping; D003432:Cross-Linking Reagents; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012333:RNA, Messenger / Q000096:biosynthesis; D011869:Radioligand Assay; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000235:genetics*; D017472:Receptors, Interleukin-1
D003432:Cross-Linking Reagents; D007375:Interleukin-1; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D004247:DNA