Biochemical and immunohistochemical evidence for selective expression of novel epithelial lipoxygenases.
Human tracheal epithelial cells contain an arachidonate 15-lipoxygenase, while the same cells from animals (including bovine, ovine, canine, porcine) cells express a 12-lipoxygenase. The epithelial 12-lipoxygenase is antigenically related to the leukocyte 12-lipoxygenase but is biochemically distinct from platelet and leukocyte forms of the enzyme, in that it is more efficient at metabolizing a wider array of fatty acid substrates. We have suggested that this lipoxygenase heterogeneity may provide a basis for different functional roles for the enzyme in different cell types. In addition, animal epithelial 12-lipoxygenase and human epithelial 15-lipoxygenase are antigenically related and have similar but distinct distributions in the lung. Our findings might suggest that the species diversity for epithelial lipoxygenases represents molecular divergence within a family of closely related genes with perhaps closely related functions.
Shannon
V R
VR
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
Hansbrough
J R
JR
Takahashi
Y
Y
Ueda
N
N
Yamamoto
S
S
Holtzman
M J
MJ
D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000032:analysis* / Q000276:immunology; D001093:Arachidonate 15-Lipoxygenase / Q000032:analysis* / Q000276:immunology; D002417:Cattle; D004285:Dogs; D004790:Enzyme Induction; D004848:Epithelium / Q000201:enzymology*; D006801:Humans; D012756:Sheep; D013045:Species Specificity; D013552:Swine; D014132:Trachea / Q000201:enzymology
D001092:Arachidonate 12-Lipoxygenase; D001093:Arachidonate 15-Lipoxygenase