title: Ultrastructural cytochemical characterization of alpha-naphthyl acetate esterase in chicken monocytic cell lines.
abstract: In order to study the differentiation level of two chicken monocytic cell lines, IN24 and LSCC-NP1 cells, alpha-naphthyl acetate esterase (ANAE) was examined by ultrastructural cytochemical technique. Morphologically and functionally, IN24 cells showed immature character compared to LSCC-NP1 cells. In IN24 and LSCC-NP1 cells, ANAE activity was localized on the external face of the plasma membrane and coated vesicles, but not in lysosomes and phagocytic vacuoles. ANAE reactivity showed a dense linear reaction product on the entire cell surface in IN24 cells and granular reaction product in LSCC-NP1 cells. On treatment with interferon-gamma (IFN-gamma), ANAE reactivity of IN24 cells became similar to that of LSCC-NP1 cells. IN24 cells treated with lipopolysaccharide (LPS) had less amounts of ANAE surface product, and reactivity was partially dispersed on the cell surface.
authors.affiliation: Department of Veterinary Pathology, Faculty of Agriculture, Yamaguchi University, Yoshida, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Inoue
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: H
authors.initials: H
authors.lastname: Hihara
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D002645:Chickens; D006651:Histocytochemistry; D009000:Monocytes / Q000201:enzymology* / Q000648:ultrastructure; D009283:Naphthol AS D Esterase / Q000032:analysis*
chemical_list: D009283:Naphthol AS D Esterase