Direct binding of protein A, protein G, and anti-IgG conjugates to chicken infectious anemia virus.
Recombinant Protein A, recombinant Protein G, and anti-chicken-IgG anti-bodies raised in rabbits, goats, or horses were found to bind directly to chicken infectious anemia virus (CIAV). MSB-1 cells infected with the Cux-1 strain of chicken anemia agent, but not to uninfected MSB-1 cells were found to react with fluorescein isothiocyanate conjugates. In an enzyme-linked immunosorbent assay, rabbit anti-chicken horseradish peroxidase conjugate bound directly to CIA-1 CIAV-coated plates. In addition, sera from a low percentage of specific-pathogen-free breeder hens reacted in an indirect fluorescent antibody test to detect CIAV antibodies. These reactions generally disappeared within a month. The breeder flocks were demonstrated to be free of CIAV infection by the susceptibility of their progeny.
Lucio
B
B
Department of Avian and Aquatic Animal Medicine, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Schat
K A
KA
Taylor
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S
D000740:Anemia / Q000382:microbiology / Q000662:veterinary; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D001667:Binding, Competitive; D002645:Chickens; D004198:Disease Susceptibility; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D005455:Fluorescent Antibody Technique; D007074:Immunoglobulin G / Q000378:metabolism*; D011201:Poultry Diseases / Q000382:microbiology; D011994:Recombinant Proteins / Q000378:metabolism; D013047:Specific Pathogen-Free Organisms; D013205:Staphylococcal Protein A / Q000378:metabolism*; D014777:Virus Diseases / Q000382:microbiology / Q000662:veterinary; D014780:Viruses / Q000378:metabolism*
D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D013205:Staphylococcal Protein A