Development of murine monoclonal antibodies against an enterotoxin produced by Bacillus cereus.
Three murine monoclonal antibodies (MAbs) were prepared against an enterotoxin (ET) produced by Bacillus cereus. Although these MAbs were found to react with the ET, their specificities appeared to be different in competitive binding assays. One of the MAbs (D-8), which was highly reactive with the ET, will be useful in developing immunological methods to detect crude ET and to isolate the ET in high yield.
Shinagawa
K
K
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Japan.
Yokoi
R
R
Matsusaka
N
N
Sugii
S
S
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D001409:Bacillus cereus; D015153:Blotting, Western; D004768:Enterotoxins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005517:Foodborne Diseases / Q000382:microbiology; D006825:Hybridomas; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D004768:Enterotoxins