Molecular cloning of cDNA encoding the 16 KDa subunit of vacuolar H(+)-ATPase from mouse cerebellum.
cDNA for the 16 kDa subunit of vacuolar H(+)-ATPase was cloned from mouse cerebellum and sequenced. The deduced polypeptide (155 amino acid residues; molecular weight, 15,808) was highly hydrophobic and homologous to the subunits of bovine adrenal medulla, Torpedo marmorata electric lobe, Drosophila and yeast. Glu-139 (supposed to be essential for proton transport) was also conserved as the potential dicyclohexylcarbodiimide binding site. The subunit had four transmembrane segments: Segment II and IV were highly homologous and Glu-139 was located in Segment IV. The roles of the non-conserved regions are discussed.
Hanada
H
H
Department of Organic Chemistry and Biochemistry, Osaka University, Japan.
Hasebe
M
M
Moriyama
Y
Y
Maeda
M
M
Futai
M
M
D000313:Adrenal Medulla / Q000201:enzymology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002417:Cattle; D002531:Cerebellum / Q000201:enzymology*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004330:Drosophila / Q000201:enzymology; D004557:Electric Organ / Q000201:enzymology; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D046911:Macromolecular Substances; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015345:Oligonucleotide Probes; D006180:Proton-Translocating ATPases / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012689:Sequence Homology, Nucleic Acid; D014101:Torpedo; D014617:Vacuoles / Q000201:enzymology*
D046911:Macromolecular Substances; D015345:Oligonucleotide Probes; D004247:DNA; D006180:Proton-Translocating ATPases