Immunoassay method for the determination of immunoglobulin G using bacterial magnetic particles.
We have developed a novel immunoassay method using bacterial magnetic particles for the determination of immunoglobulin G (IgG). Fluorescein isothiocyanate (FITC) conjugated anti IgG-bacterial magnetic particles were prepared. The fluorescence quenching caused by agglutination of FITC-anti IgG antibody-bacterial magnetic particle conjugates was measured by using a fluorescence spectrophotometer. The aggregates based on specific immunoreaction were separated by a gelatin solution. The aggregation of bacterial magnetic particle conjugates was enhanced by application of a magnetic field. The relative fluorescence intensity correlated linearly with a concentration of IgG in the range 0.5-100 ng/mL.
Nakamura
N
N
Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
Hashimoto
K
K
Matsunaga
T
T
D000371:Agglutination; D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D001419:Bacteria / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate; D015200:Fluoroimmunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis*; D008280:Magnetics; D051379:Mice
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D016650:Fluorescein-5-isothiocyanate