Isolation and chromosomal localization of a novel nonerythroid ankyrin gene.
Immunoreactive isoforms of erythrocyte ankyrin have been shown to be present in a variety of nonerythroid tissues. Isolation of the genes that encode these isoforms will clarify their relationship to erythrocyte ankyrin. Using an erythrocyte ankyrin cDNA clone as a hybridization probe, we screened a human genomic library and isolated a clone that hybridizes with the probe at low stringency but not at high stringency. Partial nucleotide sequence of the clone revealed the presence of a 99-bp segment that is homologous to an exon of the erythrocyte ankyrin gene. Northern analysis showed that a labeled fragment of the clone hybridized to a 7-kb message in RNA of fetal brain but not of erythroid cells, suggesting that this clone is part of a novel gene that is expressed predominantly in nonerythroid tissue. Comparison of the sequence of the genomic clone with that of a recently isolated cDNA clone for brain ankyrin (Otto et al., 1989) showed identity of 96 of 99 bp between the putative exon and a segment of the cDNA clone (V. Bennett, personal communication, 1991), suggesting that the genomic clone is part of a gene for nonerythroid ankyrin, which we have designated ANK2. By analysis of somatic cell hybrids and fluorescence in situ hybridization, we assigned ANK2 to human chromosome 4 at a position equivalent to bands 4q25-q27.
Tse
W T
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Department of Human Genetics, Yale University School of Medicine, New Haven, Connecticut 06510.
Menninger
J C
JC
Yang-Feng
T L
TL
Francke
U
U
Sahr
K E
KE
Lux
S E
SE
Ward
D C
DC
Forget
B G
BG
D000595:Amino Acid Sequence; D000818:Animals; D017487:Ankyrins; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics*; D002460:Cell Line; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004912:Erythrocytes / Q000502:physiology*; D005091:Exons; D005796:Genes; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D015183:Restriction Mapping
D017487:Ankyrins; D001798:Blood Proteins; D008565:Membrane Proteins; D004247:DNA