Phosphatase production by a Citrobacter sp. growing in batch cultures retarded by anaerobic or osmotic stress, and the effect of the osmoprotectant glycine betaine.
The effect of 500 mM NaCl on the growth, and phosphatase production of a Citrobacter sp. was investigated. Although growth was retarded, phosphatase production was enhanced by 50%. Relief from osmotic stress using the osmoprotectant glycine betaine gave normal growth, but phosphatase activity was reduced. The Citrobacter sp. ceased to grow following a shift to anaerobic conditions, but anaerobically-incubated cells continued to produce phosphatase after a transient lag.
Hallett
D S
DS
Department of Biochemistry, University of Oxford, U.K.
Clark
P
P
Macaskie
L E
LE
D000693:Anaerobiosis; D001622:Betaine / Q000494:pharmacology*; D002954:Citrobacter / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D006706:Homeostasis; D007700:Kinetics; D009997:Osmotic Pressure; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D014882:Water-Electrolyte Balance
D001622:Betaine; D012965:Sodium Chloride; D010744:Phosphoric Monoester Hydrolases