Detection of primary Epstein-Barr virus infection in a patient with X-linked lymphoproliferative disease receiving immunoglobulin prophylaxis.
Serologic diagnosis for Epstein-Barr virus (EBV) infection is problematic when patients receive exogenous immunoglobulin. We recently diagnosed primary EBV infection by detecting EBV-determined nuclear antigen (EBNA) and EBV-DNA in peripheral blood mononuclear cells (PBMC) using immunofluorescence, in situ hybridization and polymerase chain reaction (PCR) techniques in a patient with X-linked lymphoproliferative disease (XLP) who received prophylactic immunoglobulins for EBV infection. These combined techniques may be helpful for early and accurate diagnosis of EBV infection in highly vulnerable patients.
Okano
M
M
Department of Pathology, University of Nebraska Medical Center, Omaha.
Bashir
R M
RM
Davis
J R
JR
Purtilo
D T
DT
D000328:Adult; D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000150:complications / Q000175:diagnosis* / Q000517:prevention & control; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D019309:Epstein-Barr Virus Nuclear Antigens; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007116:Immunization, Passive; D008232:Lymphoproliferative Disorders / Q000150:complications / Q000188:drug therapy* / Q000382:microbiology; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization
D000956:Antigens, Viral; D004279:DNA, Viral; D019309:Epstein-Barr Virus Nuclear Antigens