Primary structure of the alpha-subunit of vacuolar-type Na(+)-ATPase in Enterococcus hirae. Amplification of a 1000-bp fragment by polymerase chain reaction.
A 1000-bp fragment of Enterococcus hirae genomic DNA was amplified by the polymerase chain reaction method, using the oligonucleotide primers designed from amino acid sequences of both amino-terminal and a tryptic fragment of the Na(+)-ATPase alpha-subunit in this organism. DNA sequencing of this product revealed that the amino acid sequence of Na(+)-ATPase alpha-subunit is highly homologous to the corresponding sequences of large (alpha) subunits of vacuolar (archaebacterial) type H(+)-ATPases, supporting our proposal [Kakinuma, Y. and Igarashi, K. (1990) FEBS Lett. 271, 97-101] that the Na(+)-ATPase of this organism belongs to the vacuolar-type ATPase.
Kakinuma
Y
Y
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Igarashi
K
K
Konishi
K
K
Yamato
I
I
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D027682:Cation Transport Proteins; D004269:DNA, Bacterial / Q000235:genetics; D016983:Enterococcus / Q000201:enzymology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D014617:Vacuoles / Q000201:enzymology*
D027682:Cation Transport Proteins; D004269:DNA, Bacterial; D000251:Adenosine Triphosphatases; C021838:sodium-translocating ATPase