Lysogeny of methicillin-resistant Staphylococcus aureus and the role of prophages in transfer of conjugative and non-conjugative plasmids.
The lysogenicity of 49 strains of methicillin-resistant S. aureus (MRSA) isolated in Moscow clinics in the 1970s and '80s was studied by the method of mitomycin C induction. It was found that one strain had phage of serogroup B, 33 strains had serogroup F phages and 15 strains had phages of both serogroups. In the course of genetic crossing on nitrocellulose filters it was demonstrated that serogroups B and F prophages contained in recipient cells 1) increase the frequency of transfer of conjugative plasmid pG873 and 2) mobilize transfer of non-conjugative plasmids pE994 and rms7.
Zueva
V S
VS
Laboratory of Staphylococcal Infections, Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, USSR.
Nesterenko
L N
LN
Dmitrenko
O A
OA
Akatov
A K
AK
D003227:Conjugation, Genetic; D006801:Humans; D008242:Lysogeny / Q000235:genetics*; D016106:Methicillin Resistance; D010957:Plasmids / Q000235:genetics; D013203:Staphylococcal Infections / Q000382:microbiology; D013204:Staphylococcus Phages / Q000145:classification / Q000235:genetics* / Q000502:physiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000502:physiology