Expression of TNF-alpha and TNF-beta transcripts in murine embryonal carcinoma cells and trophoblast cell.
Using a combination system of Transcription and PCR, we examined gene expression of TNF-alpha, TNF-beta and other cytokines (IL-1, G-CSF, IL-3 and IFN-gamma) in embryonal carcinoma cell lines (PCC3, PCC4, ECA2 and F9) and trophoblast cell line (PL/B6). We found that both TNF-alpha and TNF-beta transcripts were expressed in all of the embryonic cell lines. We also detected transcripts of two types of TNF receptors and transcription factor NF-kappa B in these embryonic cells.
Kohchi
C
C
Biotechnology Research Center, Teikyo University, Kawasaki, Japan.
Mizuno
D
D
Soma
G
G
D000818:Animals; D054278:Embryonal Carcinoma Stem Cells; D015870:Gene Expression; D008233:Lymphotoxin-alpha / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D014158:Transcription, Genetic; D014327:Trophoblasts / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics
D008233:Lymphotoxin-alpha; D014409:Tumor Necrosis Factor-alpha