Seasonal pattern of glycosylation in frog liver.
The circannual behaviour of glycosylation and protein synthesis in frog liver slices was studied following the incorporation of 3H-galactose and 14C-glucosamine into glycolipids and glycoproteins and 3H-leucine into proteins. The activity of two enzymes the galactosyl-transferase and the N-acetyl-glucosaminyl-1-P-transferase was determined. The incorporations of both sugars into the soluble fraction and into the lipid extract present a maximum during the spring-summer period. The incorporation into the protein fraction displays a different pattern: 14C-Glucosamine and 3H-leucine incorporation increases from winter to a maximum in autumn; the incorporation of 3H-Galactose has a sharp peak during spring. The pattern of glycosyltransferase activities is similar to the pattern of incorporation of the two saccharides into proteins, indicating these enzymes as important control points for glycosylation in Anurae.
Leoni
S
S
Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Roma La Sapienza, Italy.
D'Alessandro
A
A
Conti
R
R
Marino
M
M
Spagnuolo
S
S
Mangiantini
M T
MT
D000818:Animals; D005690:Galactose / Q000378:metabolism; D005700:Galactosyltransferases / Q000378:metabolism*; D005944:Glucosamine / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism*; D006017:Glycolipids / Q000096:biosynthesis; D006023:Glycoproteins / Q000096:biosynthesis*; D006031:Glycosylation; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D017351:N-Acetylglucosaminyltransferases; D014176:Protein Biosynthesis; D011893:Rana esculenta; D012621:Seasons
D006017:Glycolipids; D006023:Glycoproteins; D005700:Galactosyltransferases; D005964:Glucosyltransferases; D017351:N-Acetylglucosaminyltransferases; C037649:N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase; D007930:Leucine; D005944:Glucosamine; D005690:Galactose