title: Influence of decorin on fibroblast adhesion to fibronectin.
abstract: Decorin is a ubiquitous small dermatan sulfate proteoglycan carrying a single glycosaminoglycan chain. It is known for its ability to bind, via its core protein, to interstitial collagens. Decorin was purified from the secretions of cultured human skin fibroblasts under non-denaturing conditions. The intact proteoglycan and its glycosaminoglycan-free core protein were tested for their interference with fibroblast adhesion to a fibronectin substrate. Concentrations of 40 nmoles or more of hexuronic acid/ml of decorin or equivalent amounts of core protein inhibited cell adhesion. Inhibition was caused by an interaction of core protein with fibronectin and not by masking of the fibronectin receptor. When cell-binding fragments of fibronectin were used as substrates, a similar inhibition of cell adhesion by decorin core protein was found, and in vitro assays demonstrated an interaction of core protein with the cell-binding domain of fibronectin. Decorin core protein also inhibited the low degree of cell adhesion to heparin-binding fragments on the N-terminus and near the C-terminus of the fibronectin molecules.
authors.affiliation: Institut für Physiologische Chemie und Pathobiochemie Universität, Münster/Bundesrepublik Deutschland.
authors.forename: M
authors.initials: M
authors.lastname: Winnemöller
authors.forename: G
authors.initials: G
authors.lastname: Schmidt
authors.forename: H
authors.initials: H
authors.lastname: Kresse
mesh_terms: D002448:Cell Adhesion; D002478:Cells, Cultured; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005347:Fibroblasts / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D011509:Proteoglycans / Q000378:metabolism*; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000378:metabolism
chemical_list: C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D011509:Proteoglycans; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic