Indomethacin increases the sensitivity of the monocyte migration inhibition assay.
In ophthalmo-immunological investigations only small samples of ocular tissues and fluid are available and assays which are feasible with very small volumes or cell numbers are mandatory. Indomethacin, which is known to augment the immune response both in vivo and in vitro was therefore tested for its effect on the monocyte migration inhibition (MIF) assay using low cell or antigen doses. The sensitivity of the MIF assay may be greatly increased by adding indomethaci during the first step of the assay. Titration of either the antigen dose, the mononuclear cells number or both per assay, resulted in a 10-50-fold increase in sensitivity of the assay, with a broad inter-individual variability. Increasing the sensitivity of the MIF assay with indomethacin has clear advantages with regard to the number of cells required but also confronts us with a new problem: activation of specific cells that circulate at very low frequencies in non-immunized individuals. The enhanced response could be reversed to some extent by adding prostaglandin E2 together with indomethacin to the first step of the assay. Moreover, adding leukotriene B4 to the first step of the assay had an enhancing effect over a limited concentration range. We conclude that in the presence of indomethacin, the MIF assay provides a highly sensitive technique for the demonstration of cellular immune responses in small samples of biological fluids containing very small numbers of antigen-specific lymphocytes.
Van der Gaag
R
R
Department of Ophthalmo-Immunology, Netherlands Ophthalmic Research Institute, Amsterdam.
Broersma
L
L
De Hon
F D
FD
Van Haren
M A
MA
Hoekzema
R
R
D002464:Cell Migration Inhibition; D015232:Dinoprostone / Q000494:pharmacology; D006801:Humans; D007114:Immunization; D007213:Indomethacin / Q000494:pharmacology*; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis; D007975:Leukotriene B4 / Q000494:pharmacology; D009000:Monocytes / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology
D007961:Leukocyte Migration-Inhibitory Factors; D014373:Tuberculin; D007975:Leukotriene B4; D015232:Dinoprostone; D007213:Indomethacin