Mutation spectrum of copper-induced DNA damage.
The ability of metal ions to damage DNA and cause mutagenesis has been analyzed with reversion and forward mutation assays using single-stranded DNA templates. We previously reported that incubation of phi X174 am3 DNA with Fe2+ in vitro results in mutagenesis when the treated DNA is transfected into Escherichia coli spheroplasts (Loeb, L. A., James, E. A., Waltersdorph, A. M., and Klebanoff, S. J. (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 3918-3922, 1988). We now extend these studies to other metal ions. Of the metal ions tested, copper ions were the most mutagenic; the frequency of mutants produced was equal to or greater than that produced by Fe2+. Mutagenesis by Cu+ was diminished by catalase, mannitol, and superoxide dismutase suggesting the involvement of H2O2, hydroxyl ions, and superoxide, respectively. However, the findings that Cu+ and Cu2+ are nearly equally mutagenic and that the mutagenic activities are not completely inhibited by oxygen free radical scavengers make it unlikely that the mechanism for mutagenesis is simply the production of hydroxyl free radicals. The spectra of mutations produced by either copper ion using the lacZ gene as a target are very similar and differ from those reported with other agents. The predominant mutagenic sequence changes are single-base substitutions, the most frequent being replacement of a template C by a T. This transition presumably results from mispairing of an altered C with deoxyadenosine. Copper-induced mutations are not randomly distributed. Instead, they are found predominantly in clusters suggesting direct interaction of copper ions with specific nucleotide sequences in DNA. Evidence is considered that the high frequency of C----T transitions may be a common manifestation of DNA damage by oxygen radicals.
Tkeshelashvili
L K
LK
Joseph Gottstein Memorial Cancer Research Laboratory, Department of Pathology, University of Washington, School of Medicine, Seattle 98195.
McBride
T
T
Spence
K
K
Loeb
L A
LA
D010584:Bacteriophage phi X 174 / Q000235:genetics; D001483:Base Sequence; D003300:Copper / Q000633:toxicity*; D004249:DNA Damage; D004269:DNA, Bacterial / Q000187:drug effects* / Q000235:genetics; D004279:DNA, Viral / Q000187:drug effects* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009152:Mutagenicity Tests; D009154:Mutation; D014162:Transfection
D004269:DNA, Bacterial; D004279:DNA, Viral; D003300:Copper