[Gene diagnosis of hemophilia A by PCR].
DNA fragments containing restriction enzyme Bcl I or XbaI polymorphic sites within the gene of coagulation factor VIII were amplified, respectively. Carrier detection and prenatal diagnosis of hemophilia A were performed by analysis of the polymorphisms of the amplified DNA. This is a simple, fast and sensitive technique, especially for prenatal diagnosis with very small amounts of chorionic villi or amniocytes. Four families with hemophilia A were analyzed, and 3 cases at high risk for hemophilia A were diagnosed prenatally.
Feng
J
J
Institute of Basic Medical Sciences, Beijing.
D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis*; D006467:Hemophilia A / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D015345:Oligonucleotide Probes; D010375:Pedigree; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*
D015345:Oligonucleotide Probes