Cyclic AMP response element-binding protein, CRE-BP1, mediates the E1A-induced but not the Tax-induced trans-activation.
The adenovirus E1A protein and tax protein (Tax) of human T-cell leukemia virus-1 (HTLV-1) are transcriptional regulators that do not bind to DNA directly. The ATF sites/CRE (cyclic AMP response element) of the adenovirus E4 promoter and the long terminal repeat of HTLV-1 have been shown to be required for E1A and Tax inducibility, respectively. Using the c-Myb-CRE-BP1 fusion protein, it was shown that CRE-BP1, which could bind to the ATF sites/CRE, mediated the E1A-induced trans-activation. For this activation, the N-terminal portion of CRE-BP1, which contained the putative metal finger structure, was essential but not sufficient. In contrast, the trans-activation induced by HTLV-1 Tax was not mediated by CRE-BP1. These results strongly suggested that E1A activates transcription through interaction with CRE-BP1, but another CRE-binding protein participates in the Tax-induced trans-activation.
Maekawa
T
T
Laboratory of Molecular Genetics, Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Matsuda
S
S
Fujisawa
J
J
Yoshida
M
M
Ishii
S
S
D017456:Adenovirus Early Proteins; D002874:Chromosome Mapping; D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D016356:Gene Products, tax; D066298:In Vitro Techniques; D009856:Oncogene Proteins, Viral; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000494:pharmacology; D020598:Proto-Oncogene Proteins c-myb; D014157:Transcription Factors; D015533:Transcriptional Activation / Q000187:drug effects*; D014162:Transfection; D014760:Viral Fusion Proteins
D017456:Adenovirus Early Proteins; D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins; D016356:Gene Products, tax; D009856:Oncogene Proteins, Viral; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D014157:Transcription Factors; D014760:Viral Fusion Proteins