Construction and analysis of deletions in the structural gene (uvrD) for DNA helicase II of Escherichia coli.
DNA helicase II, the product of the uvrD gene, has been implicated in DNA repair, replication, and recombination. Because the phenotypes of individual uvrD alleles vary significantly, we constructed deletion-insertion mutations in the uvrD gene to determine the phenotype of cells lacking DNA helicase II. Deletion mutants completely lacking the protein, as well as one which contains a truncated protein retaining the ATP-binding site, remained viable. However, they were sensitive to UV light and exhibited elevated levels of homologous recombination and spontaneous mutagenesis. In addition, mutations mapping in or near rep which allow construction of rep uvrD double mutants at a high frequency were isolated.
Washburn
B K
BK
Department of Genetics, University of Georgia, Athens 30602.
Kushner
S R
SR
D000251:Adenosine Triphosphatases / Q000235:genetics*; D015139:Blotting, Southern; D002470:Cell Survival / Q000528:radiation effects; D002872:Chromosome Deletion; D004265:DNA Helicases / Q000235:genetics*; D004260:DNA Repair; D004264:DNA Topoisomerases, Type I / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000235:genetics; D010641:Phenotype; D010957:Plasmids; D011836:Radiation Tolerance / Q000235:genetics; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014161:Transduction, Genetic; D014466:Ultraviolet Rays / Q000009:adverse effects
D029968:Escherichia coli Proteins; C506744:rep protein, E coli; D000251:Adenosine Triphosphatases; C506746:UvrD protein, E coli; D004265:DNA Helicases; D004264:DNA Topoisomerases, Type I