title: CTD kinase associated with yeast RNA polymerase II initiation factor b.
abstract: A kinase activity specific for the C-terminal repeat domain (CTD) of RNA polymerase II is associated with nearly homogeneous yeast general initiation factor b by three criteria: cofractionation on the basis of size and charge and coinactivation by mild heat treatment. The kinase phosphorylates the CTD at multiple sites in a processive manner. Factor b may possess a DNA-dependent ATPase activity as well. Both kinase and DNA-dependent ATPase activities exhibit the same nucleotide requirements as previously demonstrated for the initiation of transcription. These results support the idea that phosphorylation of the CTD lies on the pathway of transcription initiation and identify a catalytic activity of a general factor essential for the initiation process.
authors.affiliation: Department of Cell Biology, Stanford University School of Medicine, California 94305.
authors.forename: W J
authors.initials: WJ
authors.lastname: Feaver
authors.forename: O
authors.initials: O
authors.lastname: Gileadi
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: R D
authors.initials: RD
authors.lastname: Kornberg
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000595:Amino Acid Sequence; D004265:DNA Helicases; D004252:DNA Mutational Analysis; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011494:Protein Kinases / Q000502:physiology*; D012319:RNA Polymerase II / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013379:Substrate Specificity; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D046911:Macromolecular Substances; D014157:Transcription Factors; D011494:Protein Kinases; D012319:RNA Polymerase II; D000251:Adenosine Triphosphatases; D004265:DNA Helicases