Characterization of immunomodulating action of TI-31 on antibody response in mice.
TI-31, at concentrations of 10 and 100 microM, suppressed the antibody response to T cell-dependent (sheep red blood cells, SRBC) and -independent antigens (trinitrophenyl-lipopolysaccharide, TNP-LPS and TNP-Ficoll). The anti-SRBC response was suppressed by TI-31 when T cells were replaced by a supernatant of concanavalin A (Con A)-stimulated spleen cells, indicating the drug had influence on T lymphocytes, B lymphocytes and macrophages. TI-31 augmented the Con A-induced suppressor T cell (Ts) assessed by anti-SRBC plaque-forming cells response in vitro. The drug (10 mg/kg, p.o.) potentiated antigen specific Ts induction in vitro after injection of a high dose of SRBC. The results demonstrate that TI-31 enhanced Ts induction, which resulted in a diminution of antibody formation.
Komoriya
K
K
Pharmacological Research Department, Teijin Institute for Bio-medical Research II, Tokyo, Japan.
Nagata
I
I
Takeshita
T
T
Yamamoto
I
I
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D003208:Concanavalin A / Q000494:pharmacology; D003864:Depression, Chemical; D006462:Hemolytic Plaque Technique; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology; D013844:Thiazoles / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D013844:Thiazoles; D003208:Concanavalin A; C038415:TEI 3096