title: P regulatory products repress in vivo the P promoter activity in P-lacZ fusion genes.
abstract: The transposition of P elements in Drosophila melanogaster is regulated by products encoded by the P elements themselves. The molecular mechanisms of this regulation are complex and still unclear. We have assayed in vivo the effects of P regulatory products on the P promoter itself by using P-lacZ fusion genes. We have found that all the P-lacZ insertions are repressed in a P background. This repression occurs in all the tissues observed and at all the developmental stages. The amount of transcripts specific for P-lacZ is substantially reduced in a P background. These results suggest that P trans-acting products can exert a direct repression on the P promoter transcription.
authors.affiliation: Mécanismes moléculaires de la spéciation, Université Pierre et Marie Curie, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Lemaitre
authors.forename: D
authors.initials: D
authors.lastname: Coen
mesh_terms: D000818:Animals; D004274:DNA, Recombinant; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005786:Gene Expression Regulation; D009713:Nucleotidyltransferases / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D019895:Transposases; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D004274:DNA, Recombinant; D009713:Nucleotidyltransferases; D019895:Transposases; D001616:beta-Galactosidase