title: Discrimination between mammalian RNases H-1 and H-2.
abstract: The two principal RNases H in mammalian cells, H-1 and H-2, differ in their responses to sale, divalent metal, and sulfhydryl inhibition. Specific reaction conditions that provide unambiguous discrimination between RNases H-1 and H-2 with only two assays are described. The assays were used for identification in a new purification procedure for RNases H-1 and H-2.
authors.affiliation: Department of Medicine, University of California, San Diego, La Jolla 92093-0613.
authors.forename: M
authors.initials: M
authors.lastname: Goulian
authors.forename: C J
authors.initials: CJ
authors.lastname: Heard
mesh_terms: D000818:Animals; D004722:Endoribonucleases / Q000302:isolation & purification*; D008722:Methods; D051379:Mice; D016914:Ribonuclease H; D014407:Tumor Cells, Cultured
chemical_list: D004722:Endoribonucleases; D016914:Ribonuclease H