Inactivation of viruses in platelet suspensions that retain their in vitro characteristics: comparison of psoralen-ultraviolet A and merocyanine 540-visible light methods.
The ability of two fundamentally different photochemical procedures to inactivate model viruses in platelet suspensions was compared. Merocyanine 540 (MC 540) with visible light was used as an example of an oxygen-dependent chemical-directed at the viral membrane, and aminomethyl trimethyl psoralen (AMT) with ultraviolet A light (UVA) was used as an example of a nucleic acid-directed system. Antiviral conditions in petri dishes were identified and the effects of these procedures on platelet suspensions in plastic storage containers were studied. Concentrations of photochemicals in the 10 to 150 mumol range with 30 to 60 minutes of visible light (MC 540) or 1 to 2 minutes of UVA (AMT) readily inactivated 5 to 6 log10 of vesicular stomatitis virus (VSV) and other model viruses in platelet suspensions, provided the plasma concentration was reduced to about 15 percent by the use of a synthetic platelet storage medium. Extracellular pH, morphology scores, and aggregation response dropped markedly when platelets were treated with MC 540 and visible light. However, treatment with 136 mumol per L of AMT and 1 to 3 minutes of UVA could inactivate 5 log10 of VSV in platelet suspensions with retention of platelet characteristics for 4 days, particularly if oxygen levels were reduced during treatment. These studies demonstrate that AMT-UVA treatment meets the initial requirements for virus inactivation in platelet suspensions.
Dodd
R Y
RY
Jerome H. Holland Laboratory, American Red Cross, Rockville, Maryland.
Moroff
G
G
Wagner
S
S
Dabay
M H
MH
Dorfman
E
E
George
V
V
Ribeiro
A
A
Shumaker
J
J
Benade
L E
LE
D001792:Blood Platelets / Q000382:microbiology*; D006801:Humans; D008027:Light; D011701:PUVA Therapy; D010778:Photochemotherapy; D011744:Pyrimidinones / Q000494:pharmacology; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology; D014775:Virus Activation / Q000187:drug effects*
D011744:Pyrimidinones; D011838:Radiation-Sensitizing Agents; C003954:merocyanine dye