[Design of a gene coding encoding a polypeptide, mixing the enzyme activity of Escherichia coli homoserine kinase and threonine synthetase].
The functioning of Escherichia coli threonine operon isolated genes thrB and thrC was studied by using the genetic complementation and enzymatic activity determination techniques. A new gene thrBC was obtained by the genes merging. The genes thrB and thrC were shown to function in Escherichia coli cells independent of the operon and the polipeptide encoded by the thrBC gene combined the functions to express the products of both genes in bacterial cell. At the same time the enzyme coded for by the merged genes demonstrates the level of activity compared with the ones of the isolated genes.
Manucharian
K G
KG
Gening
L V
LV
Gusiatiner
M M
MM
Gazarian
K G
KG
D000595:Amino Acid Sequence; D001483:Base Sequence; D019757:Carbon-Oxygen Lyases; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology*; D008190:Lyases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D010455:Peptides / Q000235:genetics*; D010770:Phosphotransferases / Q000235:genetics / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor); D010957:Plasmids
D010455:Peptides; D004247:DNA; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021370:homoserine kinase; D008190:Lyases; D019757:Carbon-Oxygen Lyases; C023160:threonine synthase
Konstruirovanie gena, kodiruiushchego polipeptid, sovmeshchaiushchiĭ fermentativnye aktivnosti gomoserinkinazy i treoninsintetaty Escherichia coli.