title: Hirudin--a potential stabilizing factor for platelet preservation in transfusion.
abstract: The influence of additional hirudin in low doses on platelet and coagulation parameters was studied in platelet concentrates (PC) prepared by cytapheresis using citrate-dextrose solution formula A. The in vitro release of platelet factor 4 and beta-thromboglobulin was markedly lowered and morphological platelet alterations, as assessed by platelet size studies were considerably less accentuated in the presence of hirudin over a 5-day storage period; this suggests a possibly protecting effect on platelet function. No clear activation of the coagulation and fibrinolysis system was evident during PC storage with and without hirudin since thrombin-antithrombin III complexes and D-dimers did not markedly change.
authors.affiliation: Department of Clinical Haemostaseology and Transfusion Medicine, University of the Saarland, Homburg/Saar, FRG.
authors.forename: G
authors.initials: G
authors.lastname: Pindur
authors.forename: E
authors.initials: E
authors.lastname: Wenzel
authors.forename: M
authors.initials: M
authors.lastname: Koehler
authors.forename: M
authors.initials: M
authors.lastname: Heiden
authors.forename: B
authors.initials: B
authors.lastname: Braun
mesh_terms: D001777:Blood Coagulation / Q000187:drug effects; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D001793:Blood Preservation / Q000379:methods*; D019343:Citric Acid; D005069:Evaluation Studies as Topic; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D005342:Fibrinolysis / Q000187:drug effects; D005947:Glucose / Q000031:analogs & derivatives / Q000494:pharmacology; D006629:Hirudins / Q000494:pharmacology*; D006801:Humans; D010978:Platelet Factor 4 / Q000378:metabolism; D010983:Plateletpheresis; D011994:Recombinant Proteins / Q000494:pharmacology; D012636:Secretory Rate / Q000187:drug effects; D001620:beta-Thromboglobulin / Q000378:metabolism
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; D006629:Hirudins; D011994:Recombinant Proteins; D001620:beta-Thromboglobulin; C036309:fibrin fragment D; C002113:acid citrate dextrose; D019343:Citric Acid; D010978:Platelet Factor 4; D005947:Glucose