title: Modified deoxyoligonucleotides stable to exonuclease degradation in serum.
abstract: Unmodified deoxyoligonucleotides are rapidly degraded in serum-containing medium. Utilizing internally labelled deoxyoligonucleotides the deoxyribonuclease profile for fetal calf serum and human serum was determined. It was found that the predominate nuclease activity in both systems was 3' exonuclease. Deoxyoligonucleotides are protected from exonuclease degradation in sera and cell media by simple terminal modifications that maintain high binding activity for the complementary DNA sequence.
authors.affiliation: Gilead Sciences, Foster City, CA 94404.
authors.forename: J P
authors.initials: JP
authors.lastname: Shaw
authors.forename: K
authors.initials: K
authors.lastname: Kent
authors.forename: J
authors.initials: J
authors.lastname: Bird
authors.forename: J
authors.initials: J
authors.lastname: Fishback
authors.forename: B
authors.initials: B
authors.lastname: Froehler
mesh_terms: D001483:Base Sequence; D001769:Blood; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005092:Exonucleases / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D009841:Oligonucleotides; D005092:Exonucleases; D010100:Oxygen