2'-O-alkyloligoribonucleotides, synthesis and applications in molecular biology.
Oligo(2'-O-alkylribonucleotides) have been synthesized in which alkyl is methyl, allyl and butyl. The various phosphoramidite monomers of 2'-O-alkyl uridine, cytidine, adenosine, guanosine, inosine and 2,6-diaminopurine riboside have been synthesized from a minimum of key intermediates. Extra protection of the lactam function in uracil and hypoxanthine proves useful. The high stability of the oligomers combined with incorporation of non-radioactive reporter groups such as fluorophores, biotin and 2,4-dinitrophenylamino (DNP) moities renders them as excellent antisense tools for studying RNA processing, for locating and visualising RNA and RNP complexes in cells, for examining splicing complexes by electron microscopy and for the affinity chromatography of RNA or RNP complexes.
Sproat
B S
BS
European Molecular Biology Laboratory, Heidelberg, FRG.
Lamond
A I
AI
Garcia
R G
RG
Beijer
B
B
Pieles
U
U
Douglas
M
M
Bohmann
K
K
Carmo-Fonseco
M
M
Weston
S
S
O'Loughlin
S
S
D016375:Antisense Elements (Genetics); D001483:Base Sequence; D005456:Fluorescent Dyes; D006801:Humans; D006860:Hydrogen Bonding; D007202:Indicators and Reagents; D015345:Oligonucleotide Probes / Q000138:chemical synthesis*; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D012322:RNA Precursors; D015347:RNA Probes / Q000138:chemical synthesis; D012326:RNA Splicing; D012342:RNA, Small Nuclear / Q000378:metabolism; D017411:Ribonucleoproteins, Small Nuclear / Q000378:metabolism; D012263:Ribonucleosides / Q000138:chemical synthesis*
D016375:Antisense Elements (Genetics); D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D015345:Oligonucleotide Probes; D009843:Oligoribonucleotides; D012322:RNA Precursors; D015347:RNA Probes; D012342:RNA, Small Nuclear; D017411:Ribonucleoproteins, Small Nuclear; D012263:Ribonucleosides