Molecular cloning of cDNA for the import precursor of human coupling factor 6 of H(+)-ATP synthase in mitochondria.
The nucleotide sequence of the import precursor of coupling factor 6 (factor 6) of human H(+)-ATP synthase has been determined from a recombinant cDNA clone isolated by screening a human kidney cDNA library with a cDNA for rat factor 6 as a probe. The sequence was composed of 466 nucleotides including a coding region for the import precursor of factor 6 and noncoding regions on the 5'- and 3'-sides. The import precursor of factor 6 and its mature polypeptide deduced from the open reading frame were found to consist of 108 and 76 amino acid residues with molecular weights of 12,596 and 8,969, respectively. The presequence of 32 amino acids could be the import signal peptide for directing the protein into the mitochondrial matrix.
Higuti
T
T
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Tsurumi
C
C
Kawamura
Y
Y
Tsujita
H
H
Osaka
F
F
Yoshihara
Y
Y
Tani
I
I
Tanaka
K
K
Ichihara
A
A
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015342:DNA Probes; D004792:Enzyme Precursors / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D007668:Kidney / Q000201:enzymology; D008928:Mitochondria; D025261:Mitochondrial Proton-Translocating ATPases; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016366:Open Reading Frames; D010086:Oxidative Phosphorylation Coupling Factors; D011487:Protein Conformation; D006180:Proton-Translocating ATPases / Q000235:genetics; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D004792:Enzyme Precursors; D010086:Oxidative Phosphorylation Coupling Factors; D004247:DNA; D000251:Adenosine Triphosphatases; C000628217:F(6) ATPase; D025261:Mitochondrial Proton-Translocating ATPases; D006180:Proton-Translocating ATPases