Malignant and nonmalignant T cell lines from human T cell lymphotropic virus type I-negative patients with Sézary syndrome.
The establishment of an in vitro model for cutaneous T cell lymphomas and Sézary syndrome has been difficult since T cells from individuals with these diseases do not proliferate in response to T cell mitogens. We found that conditioned media, collected from mitogen-activated PBMC from Sézary patients, contain an IL-2 receptor inducing factor. Despite their ostensible proliferative disorder, using a combination Sézary cell-conditioned media and rIL-2, we established IL-2 responsive, human T cell lymphotropic virus type I negative T cell lines from 23 patients, nine of which contain cells with the structural and/or genetic characteristics of neoplastic Sézary T cells.
Abrams
J T
JT
Wistar Institute, Philadelphia, PA 19104-4268.
Lessin
S R
SR
Ghosh
S K
SK
Nowell
P C
PC
Ju
W
W
Vonderheid
E C
EC
Rook
A H
AH
DeFreitas
E
E
D002455:Cell Division; D002460:Cell Line / Q000166:cytology*; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D003584:Cytological Techniques; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D015750:Genes, gag; D006133:Growth Substances; D015368:Human T-lymphotropic virus 1 / Q000302:isolation & purification; D006801:Humans; D016130:Immunophenotyping; D012751:Sezary Syndrome / Q000382:microbiology / Q000473:pathology*; D012878:Skin Neoplasms / Q000382:microbiology / Q000473:pathology*; D013601:T-Lymphocytes / Q000166:cytology* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000166:cytology*
D006133:Growth Substances