Identification of the N-terminal residue of the heavy chain of both native and recombinant human hepatocyte growth factor.
The N-terminal amino acid of the heavy chain of native (purified from human plasma) and recombinant human hepatocyte growth factor (hHGF) was determined by analyses of amino acid composition and sequence of peptide fragments derived by enzymatic cleavage, peptide mapping, and fast atom bombardment mass spectrometry. Our results indicate that the N-terminal amino acid of the heavy chain of hHGF, both native and recombinant, is pyroglutamate, derived from glutamine at the 32nd residue from the initiation methionine.
Yoshiyama
Y
Y
Research Center, Mitsubishi Kasei Corporation, Yokohama, Japan.
Arakaki
N
N
Naka
D
D
Takahashi
K
K
Hirono
S
S
Kondo
J
J
Nakayama
H
H
Gohda
E
E
Kitamura
N
N
Tsubouchi
H
H
D000595:Amino Acid Sequence; D015153:Blotting, Western; D006133:Growth Substances / Q000737:chemistry*; D017228:Hepatocyte Growth Factor; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010449:Peptide Mapping; D011761:Pyrrolidonecarboxylic Acid / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry
D006133:Growth Substances; D011994:Recombinant Proteins; D017228:Hepatocyte Growth Factor; D011761:Pyrrolidonecarboxylic Acid