Complete putative metal finger and leucine zipper structures of CRE-BP1 are required for the E1A-induced trans-activation.
The adenovirus E1A protein stimulates transcription of various genes. Recent experiments using a fusion protein have shown that E1A can function through a specific CRE (cyclic AMP response element)-binding protein, CRE-BP1 (also designated ATF-2), which stimulates the transcription from a CRE-containing promoter by homodimer formation or heterodimer formation with c-Jun. In this paper, the functional domains required for mediation of the E1A-induced trans-activation were analyzed using deletion and point mutants of CRE-BP1. The mutation in the putative metal finger structure or leucine zipper structure completely abolished the ability of CRE-BP1 to mediate the E1A-induced trans-activation. Furthermore, overexpression of CRE-BP1 or c-Jun interfered with the E1A-induced trans-activation. These results suggest that the complete putative metal finger structure in the N-terminal region of CRE-BP1 plays an important role for the E1A-induced trans-activation, and the heterodimer of CRE-BP1 with the unidentified protein participates in the interaction with E1A.
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Laboratory of Molecular Genetics, Tsukuba Life Science Center, Ibaraki, Japan.
Maekawa
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Matsuda
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Ishii
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D000199:Actins / Q000235:genetics; D017456:Adenovirus Early Proteins; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002645:Chickens; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D016350:Leucine Zippers / Q000235:genetics*; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism; D009857:Oncogenes; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D015533:Transcriptional Activation; D014162:Transfection; D016335:Zinc Fingers / Q000235:genetics*
D000199:Actins; D017456:Adenovirus Early Proteins; D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins; D009856:Oncogene Proteins, Viral; D011993:Recombinant Fusion Proteins