title: Impaired autologous mixed-lymphocyte reaction of peripheral blood lymphocytes in adult periodontitis.
abstract: The autologous mixed-lymphocyte reactions (AMLR) of peripheral blood lymphocytes from 80 patients with adult periodontitis were examined. Some but not all patients showed clearly low AMLR responses; 31 of 80 subjects (39%) showing consistently low responses in AMLR (less than the mean--2 standard deviations of the healthy control group values) were designated low-AMLR patients, whereas the 42 patients (53%) who showed normal AMLR responses were designated normal-AMLR patients. However, there were no significant differences in the clinical parameters between these two groups of patients. The phenotypic analysis of T-cell fractions revealed a lower percentage of CD45RA-positive cells in CD4-positive cells (CD4+ CD45RA+ T cells) in the low-AMLR patients than those in normal-AMLR patients and healthy control subjects. No significant differences were demonstrated between the two groups in terms of the proportion of CD4-positive and CD8-positive cells in the T-cell fractions or in the expression of human leukocyte antigen DR of the monocytes and B cells in the non-T-cell fractions. In the low-AMLR patients, the allogeneic MLR was found to be normal, but the interleukin 2 production in the AMLR was found to be significantly depressed. The depressed AMLR responses and the lower percentage of CD4+ CD45RA+ T cells in the low-AMLR patients were found to be normalized following the periodontal therapy. These results might reflect changes in regulatory T-cell function induced by development of periodontal diseases.
authors.affiliation: Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kimura
authors.forename: N
authors.initials: N
authors.lastname: Fujimoto
authors.forename: H
authors.initials: H
authors.lastname: Okada
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D006649:Histocompatibility Antigens / Q000032:analysis; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D017493:Leukocyte Common Antigens; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D010518:Periodontitis / Q000276:immunology*; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D015704:CD4 Antigens; D016827:CD8 Antigens; D006684:HLA-DR Antigens; D006649:Histocompatibility Antigens; D007376:Interleukin-2; D017493:Leukocyte Common Antigens