title: Identification of a cis-acting sequence required for germ line-specific splicing of the P element ORF2-ORF3 intron.
abstract: P element transposition in Drosophila melanogaster is limited to the germ line because the third intron (the ORF2-ORF3 intron) of the P element transcript is spliced only in germ line cells. We describe a systematic search for P element sequences that are required to regulate the splicing of the ORF2-ORF3 intron. We have identified three adjacent mutations that abolish the germ line specificity and allow splicing of this intron in all tissues. These mutations define a 20-base regulatory region located in the exon, 12 to 31 bases from the 5' splice site. Our data show that this cis-acting regulatory sequence is required to inhibit the splicing of the ORF2-ORF3 intron in somatic cells.
authors.affiliation: Department of Biology, University of California, Los Angeles 90024.
authors.forename: A C
authors.initials: AC
authors.lastname: Chain
authors.forename: S
authors.initials: S
authors.lastname: Zollman
authors.forename: J C
authors.initials: JC
authors.lastname: Tseng
authors.forename: F A
authors.initials: FA
authors.lastname: Laski
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004251:DNA Transposable Elements / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005854:Germ Cells / Q000502:physiology*; D007438:Introns; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000502:physiology; D009841:Oligonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012045:Regulatory Sequences, Nucleic Acid
chemical_list: D004251:DNA Transposable Elements; D009687:Nuclear Proteins; D009841:Oligonucleotides