Chemotactic peptide from ropalidian wasp as well as the authentic chemotactic tripeptide stimulates two distinct pathways in neutrophils, but the [LYS7] analog does only one of them.
In our previous study on chemotactic peptide isolated from Ropalidian wasp, it was found to induce not only chemotaxis but also other cellular responses, such as superoxide generation and lysosomal enzyme release, but [Lys7] analog was found to induce only chemotaxis but not others. Here, studies on intracellular Ca2+ changes and receptor-binding revealed that the wasp chemotactic peptide and the authentic tripeptide stimulated two distinct receptors, but that the [Lys7] peptide could have access to only one of them.
Koike
M
M
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Nagashima
K
K
Saito
K
K
Nakanishi
M
M
Fujino
M
M
Nakajima
T
T
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002630:Chemotactic Factors / Q000302:isolation & purification / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000378:metabolism; D013329:Structure-Activity Relationship; D013481:Superoxides / Q000378:metabolism; D014863:Wasps
D002630:Chemotactic Factors; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002118:Calcium