Isolation and biochemical characterization of a zona pellucida-binding glycoprotein of boar spermatozoa.
Lectin-like molecules on the sperm surface are implicated in the process of gamete recognition and adhesion. We have isolated and biochemically characterized a 15 kDa glycoprotein from ejaculated boar sperm which possess zona pellucida-binding- and haemagglutinating-activity. The zona/15 kDa protein interaction is inhibited by fucoidan, suggesting that the glycoprotein is one of the sperm components which participate in the initial gamete interaction. N-Terminal sequence analysis of the isolated 15 kDa glycoprotein showed that it may belong to the same sperm/egg recognition-mediating protein family as the sea urchin sperm protein binding.
Jonákova
V
V
Institute of Molecular Genetics, Prague, Czechoslovakia.
Sanz
L
L
Calvete
J J
JJ
Henschen
A
A
Cechová
D
D
Töpfer-Petersen
E
E
D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D006023:Glycoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011134:Polysaccharides / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012617:Sea Urchins; D013094:Spermatozoa / Q000737:chemistry* / Q000187:drug effects; D013552:Swine; D015044:Zona Pellucida / Q000737:chemistry* / Q000187:drug effects
D006023:Glycoproteins; D011134:Polysaccharides; D011956:Receptors, Cell Surface; C035027:egg surface sperm receptor; C007789:fucoidan