cDNA cloning, characterization, and brain region-specific expression of a neuromedin-B-preferring bombesin receptor.
Recent binding studies in the central nervous system and other tissues provide evidence that the mammalian bombesin-like peptides, gastrin-releasing peptide (GRP) and neuromedin-B (NMB), exert their numerous physiological effects through at least two different receptors. We describe the structure and expression of a cloned NMB-preferring bombesin receptor (NMB-R) with properties distinct from a GRP-preferring bombesin receptor (GRP-R) reported previously. In particular, the NMB-R shows higher affinity binding to NMB than to GRP in BALB 3T3 fibroblasts expressing the cloned NMB-R. The distinct regional distribution of NMB-R and GRP-R mRNA in the brain suggests that both bombesin receptor subtypes play independent roles in mediating many of the dramatic effects of bombesin-like peptides in the central nervous system.
Wada
E
E
Laboratory of Neurochemistry, National Institute of Neurological Disorders and Stroke, National Institutes of Health Bethesda, Maryland 20892.
Way
J
J
Shapira
H
H
Kusano
K
K
Lebacq-Verheyden
A M
AM
Coy
D
D
Jensen
R
R
Battery
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D004247:DNA / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D019886:Gastrin-Releasing Peptide; D015870:Gene Expression; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D015287:Neurokinin B / Q000031:analogs & derivatives* / Q000378:metabolism / Q000502:physiology; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000378:metabolism / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018004:Receptors, Bombesin; D018042:Receptors, Neurokinin-3; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D014162:Transfection
D010455:Peptides; D012333:RNA, Messenger; D018004:Receptors, Bombesin; D018042:Receptors, Neurokinin-3; D017981:Receptors, Neurotransmitter; D019886:Gastrin-Releasing Peptide; D015287:Neurokinin B; C038393:neuromedin B; D004247:DNA