A 55 kDa plasma membrane-associated polypeptide is involved in beta-1,3-glucan synthase activity in pea tissue.
By glycerol gradient centrifugation of a detergent-solubilized plasma membrane fraction from pea tissue, we find a polypeptide of 55 kDa that copurifies with beta-1,3-glucan synthase activity. An antiserum against this polypeptide adsorbs glucan synthase activity and the 55 kDa polypeptide from digitonin-solubilized plasma membrane. These results indicate that the 55 kDa polypeptide is involved in pea beta-1,3-glucan synthase activity.
Dhugga
K S
KS
Department of Biological Sciences, Stanford University, CA 94305.
Ray
P M
PM
D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000378:metabolism*; D007887:Fabaceae / Q000201:enzymology*; D005964:Glucosyltransferases / Q000378:metabolism*; D007158:Immunologic Techniques; D008565:Membrane Proteins; D008970:Molecular Weight; D010940:Plant Proteins / Q000378:metabolism*; D010946:Plants, Medicinal; D029702:Schizosaccharomyces pombe Proteins; D012995:Solubility
D008565:Membrane Proteins; D010940:Plant Proteins; D029702:Schizosaccharomyces pombe Proteins; D005964:Glucosyltransferases; C020570:1,3-beta-glucan synthase