Coinjection strategy for visual identification of transgenic mice.
Transgenic mice were generated by coinjection of a dominant marker gene that induces fur and eye pigmentation (a tyrosinase minigene) plus an unrelated DNA construction that has a gamma-glutamyl transferase (gamma GT) promoter linked to a ras oncogene. Mice transgenic for gamma GT-ras could be identified in the first and all subsequent generations by simple visual inspection for pigmentation. Furthermore, the gamma-glutamyl transferase promoter was active in kidney but not skin of the transgenic mice, indicating that the cointegrated DNA was active and independently expressed. These results confirm that the tyrosinase minigene can be used for coinjections to allow rapid visual identification of transgenic mice.
Overbeek
P A
PA
Howard Hughes Medical Institute, Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.
Aguilar-Cordova
E
E
Hanten
G
G
Schaffner
D L
DL
Patel
P
P
Lebovitz
R M
RM
Lieberman
M W
MW
D000818:Animals; D001483:Base Sequence; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D011905:Genes, ras; D005821:Genetic Techniques; D006200:Hair Color / Q000235:genetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping
D009838:Oligodeoxyribonucleotides; D004247:DNA