title: Determinants of action potential duration in neonatal rat ventricle cells.
abstract: OBJECTIVE
  The aim was to study the currents that determine the action potential duration in ventricular cells from neonatal rats.
  
  
  METHODS
  Microelectrode measurements of action potentials from ventricle strips were compared with action potentials obtained from isolated myocytes with the whole cell patch clamp method in current clamp mode. Ionic currents were studied in myocytes in voltage clamp mode using recognised modulators of channel activity.
  
  
  METHODS
  Neonatal rats (2 d old) were decapitated and myocytes were prepared from the apical third of collagenase treated hearts.
  
  
  RESULTS
  Modification of the action potential by 1.8-5.0 mM Ca, 2.0 mM Co, 8 mM 4-aminopyridine, 1.8 mM Sr, and 20 mM tetraethylammonium suggested the presence of the slowly inactivating Ca current ICa,L, an early outward current Ieo, and at least one other K current. Action potentials from myocytes and ventricular strips were comparable. Voltage clamp experiments were confirmatory and revealed currents with the following properties: (1) ICa,L: a Ca current with a current density of 21.7 microA.cm-2, activated between -30 and -20 mV, saturated at 1.8 Cao, inactivated faster at 5 than at 1.8 mM Cao, more permeable to Ba and Sr than to Ca, and with Sr as charge carrier blocked by Ca; (2) Ieo: the peak current had a linear I/V relation between 0 and 70 mV and was abolished by 4 mM 4-aminopyridine; (3) IK1: the current was an inward rectifier that showed a relaxation at potentials negative to -90 mV.
  
  
  CONCLUSIONS
  Action potentials obtained from neonatal rat ventricle with microelectrodes are comparable with those measured in myocytes in current clamp mode. The action potential duration is mainly determined by ICa,L, Ieo, and IK1, and there is no evidence for the presence of a delayed rectifier.
authors.affiliation: Department of Physiology and Biophysics, Faculty of Medicine, University of Sherbrooke, Quebec, Canada.
authors.forename: B
authors.initials: B
authors.lastname: Fermini
authors.forename: O F
authors.initials: OF
authors.lastname: Schanne
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D002118:Calcium / Q000502:physiology; D015220:Calcium Channels / Q000502:physiology; D006352:Heart Ventricles / Q000166:cytology; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000502:physiology; D008839:Microelectrodes; D009206:Myocardium / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains; D016276:Ventricular Function / Q000502:physiology*
chemical_list: D015220:Calcium Channels; D002118:Calcium