title: Is the p29 protein involved in the rapid regulation of phosphoenolpyruvate carboxykinase (GTP)?
abstract: It has been postulated that a protein with a molecular mass of 29,000 daltons (p29), which copurifies with hepatic phosphoenolpyruvate (P-enolpyruvate) carboxykinase, forms a complex with the enzyme and stabilizes its sensitivity to Mn2+ activation by protecting critical sulfhydryl groups from oxidation (Brinkworth, R. I., Hanson, R. W., Fullin, F. A., and Schramm, V. L. (1981) J. Biol. Chem. 256, 10795-10802). In this paper we demonstrate that p29 is not only expressed in tissues which contain high amounts of P-enolpyruvate carboxykinase, such as liver and kidney, but also in brain and muscle, which have no gluconeogenic function. Furthermore, p29 is expressed in rat liver prenatally, whereas P-enolpyruvate carboxykinase is induced only after birth. The effect of p29 to protect P-enopyruvate carboxykinase against aerobic oxidation during in vitro incubation was also observed for ovalbumin and bovine albumin. Peptide sequencing of the p29 and search in a protein data bank revealed a high homology to the muscle-specific subunit of human phosphoglycerate mutase (EC 2.7.5.3). Determination of the enzyme activity confirms the identification of the p29 as the rat liver isoform of phosphoglycerate mutase. Taking all these findings together, it is concluded that this protein has no specific effect on P-enolpyruvate carboxykinase.
authors.affiliation: Abteilung für Biochemische Endokrinologie, Universitäts-Krankenhaus Eppendorf, Hamburg, Federal Republic of Germany.
authors.forename: W
authors.initials: W
authors.lastname: Höppner
authors.forename: L
authors.initials: L
authors.lastname: Beckert
authors.forename: F
authors.initials: F
authors.lastname: Buck
authors.forename: H J
authors.initials: HJ
authors.lastname: Seitz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001731:Bisphosphoglycerate Mutase / Q000378:metabolism*; D004789:Enzyme Activation; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D016415:Sequence Alignment
chemical_list: D010729:Phosphoenolpyruvate Carboxykinase (GTP); D001731:Bisphosphoglycerate Mutase