Mechanism of the immunoregulatory effect of cloned T cells on proliferation in mixed lymphocyte responses.
The mechanism of the suppression of the mixed lymphocyte response (MLR) by T cell clones derived from the murine AMLR was studied in a system employing .45 microns membrane chambers to isolate the cellular reactants. The clones effectively suppressed MLR regardless of the MHC haplotype of the reactants in the absence of direct cellular contact, via a soluble factor able to pass through this membrane. Utilizing specific neutralizing antibodies, this factor was shown to be principally TNF alpha, with a contributing effect of gamma interferon. Production of TNF alpha by the cloned T cells was confirmed by direct assay of clone culture supernatants.
Bocchieri
M H
MH
Department of Medicine, Jefferson Medical College, Thomas Jefferson University.
D000818:Animals; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured; D007108:Immune Tolerance / Q000276:immunology*; D066298:In Vitro Techniques; D016898:Interferon-alpha / Q000502:physiology; D016899:Interferon-beta / Q000502:physiology; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008814:Mice, Inbred NZB; D013601:T-Lymphocytes / Q000166:cytology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D016898:Interferon-alpha; D014409:Tumor Necrosis Factor-alpha; D016899:Interferon-beta