Specificities of rabbit anti-(2',5')oligoadenylate antibodies towards phosphorothioate and seco analogs of oligoadenylates.
Rabbit antibodies to 2',5'-linked triadenylate were prepared by immunization with (2',5')A3 conjugated via the 2'3'-levulinic group, (2'5')A3-Lev, to BSA. New radioimmunoassay for (2',5')oligoadenylates was developed using 125I thyrosine labeled derivative of (2',5')A3-Lev. Reactivity of antibodies with phosphorothioate and seco analogs of oligoadenylates was studied. It was found that (i) stereospecific substitution of the diastereotopic oxygens with sulfur in the internucleotide phosphodiester linkages changes the immunoreactivity of such analogs; (ii) the seco analogs of oligoadenylates display in some cases a rather high reactivity.
Mikhailopulo
I A
IA
Institute of Bioorganic Chemistry, Byelorussian Academy of Sciences, Minsk, USSR.
Kvasyuk
E I
EI
Kulak
T I
TI
Shulyakovskaya
S M
SM
Makarenko
M V
MV
Mikhailov
S N
SN
Charubala
R
R
Pfleiderer
W
W
D000227:Adenine Nucleotides / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity; D000998:Antiviral Agents / Q000138:chemical synthesis / Q000276:immunology*; D003429:Cross Reactions; D009843:Oligoribonucleotides / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D013329:Structure-Activity Relationship
D000227:Adenine Nucleotides; D000998:Antiviral Agents; D009843:Oligoribonucleotides; C023505:2',5'-oligoadenylate