Production of antibodies to the constant region of the mouse T-cell-receptor beta-chain.
To generate antibodies to the constant region of the mouse T-cell-receptor beta chain, the corresponding cDNA clone 86T5 was subcloned into the bacterial expression vector pEX2. The induced hybrid protein consisting of a 30 kDa T-cell-receptor segment and a beta-galactosidase carrier moiety was used for immunization. In Western blots using lysates of a T-cell-receptor-positive T-cell hybrid, the antisera obtained reacted with a 42 kDa protein under reducing and a 85 kDa protein under nonreducing conditions. The antisera did not react in binding assays with intact T lymphocytes, i.e. membrane-associated T-cell-receptor protein.
Schenkel
J
J
Institut für Immunologie, Universität Heidelberg, FRG.
Schäfer
H
H
Koch
S
S
Burger
R
R
D000818:Animals; D000917:Antibody Formation; D004247:DNA / Q000032:analysis; D051379:Mice; D011817:Rabbits; D051381:Rats; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta; D011993:Recombinant Fusion Proteins / Q000276:immunology*
D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D011993:Recombinant Fusion Proteins; D004247:DNA