title: Expression and reconstitution of a biologically active mouse interferon gamma receptor in hamster cells. Chromosomal location of an accessory factor.
abstract: In order to localize the chromosome encoding the accessory factor required for function of the murine interferon gamma (IFN-gamma) receptor, we transfected a cDNA expression vector encoding the receptor into several Chinese hamster x mouse somatic cell hybrids. As mouse Chromosome 10 carries the gene which encodes the interferon gamma-receptor (Ifgr), we used somatic cell hybrids that lack this chromosome. The presence of mouse Chromosome 16 was required to generate a response to murine IFN-gamma as assayed by stimulation of class I major histocompatibility complex antigen expression. These results demonstrate a species-specific accessory factor encoded on mouse Chromosome 16 (termed Ifgt) is necessary for mouse IFN-gamma to stimulate major histocompatibility complex expression through its receptor.
authors.affiliation: Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway 08854-5635.
authors.forename: Y
authors.initials: Y
authors.lastname: Hibino
authors.forename: T M
authors.initials: TM
authors.lastname: Mariano
authors.forename: C S
authors.initials: CS
authors.lastname: Kumar
authors.forename: C A
authors.initials: CA
authors.lastname: Kozak
authors.forename: S
authors.initials: S
authors.lastname: Pestka
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D006224:Cricetinae; D003412:Cricetulus; D005822:Genetic Vectors; D006649:Histocompatibility Antigens / Q000032:analysis; D006822:Hybrid Cells / Q000276:immunology; D007371:Interferon-gamma / Q000378:metabolism*; D008285:Major Histocompatibility Complex; D051379:Mice; D011971:Receptors, Immunologic / Q000235:genetics* / Q000276:immunology; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D014162:Transfection
chemical_list: D006649:Histocompatibility Antigens; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D007371:Interferon-gamma