Chromosomal localization of a cytochrome b5 gene to human chromosome 18 and a cytochrome b5 pseudogene to the X chromosome.
We have isolated cDNA clones that code for human cytochrome b5. Owing to the high degree of evolutionary conservation of cytochrome b5 sequences and the existence of human and rodent cytochrome b5 processed pseudogenes, we were unable to map unambiguously the chromosomal localization of the human gene(s) by Southern blot hybridization of DNA from human-rodent somatic cell hybrids. An alternative approach, based on restriction enzyme digestion of PCR-amplified DNA, enabled us to map the human cytochrome b5 gene(s) to chromosome 18 and one of its processed pseudogenes to the X chromosome. We propose the designations CYB5 and CYB5P1 for the gene and pseudogene loci, respectively.
Shephard
E A
EA
Department of Biochemistry and Molecular Biology, University College London, United Kingdom.
Povey
S
S
Spurr
N K
NK
Phillips
I R
IR
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002875:Chromosomes; D002877:Chromosomes, Human; D002887:Chromosomes, Human, Pair 18; D003001:Cloning, Molecular; D015786:Cytochromes b5 / Q000235:genetics*; D015342:DNA Probes; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011544:Pseudogenes; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D015786:Cytochromes b5