title: Approaches to some biochemical mechanisms of action of tuftsin and analogues.
abstract: Tuftsin, T-K-P-R, is a phagocytosis-stimulating peptide described as a natural immunostimulant. Four analogues of this peptide were synthesized. These compounds were assayed for their ability to compete with [3H]tuftsin for its specific receptor from thioglycollate-elicited mouse peritoneal macrophages. They were also tested for their ability to change level in intracellular cGMP and to stimulate phagocytosis through the nitroblue tetrazolium reduction measurement. Surprisingly, all the analogues were poor competitors of [3H]tuftsin binding but possess potent tuftsin-like activities.
authors.affiliation: Division de Cancérologie Expérimentale, Institut de Recherches Servier, Suresnes, France.
authors.forename: L
authors.initials: L
authors.lastname: Kraus-Berthier
authors.forename: G
authors.initials: G
authors.lastname: Ferry
authors.forename: V
authors.initials: V
authors.lastname: Combe-Perez
authors.forename: M
authors.initials: M
authors.lastname: Visalli
authors.forename: G
authors.initials: G
authors.lastname: Remond
authors.forename: M
authors.initials: M
authors.lastname: Vincent
authors.forename: J A
authors.initials: JA
authors.lastname: Boutin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D006152:Cyclic GMP / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D010587:Phagocytosis / Q000187:drug effects; D013329:Structure-Activity Relationship; D014405:Tuftsin / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000494:pharmacology*
chemical_list: D006152:Cyclic GMP; D014405:Tuftsin