title: Human bronchial epithelial cells modulate CD3 and mitogen-induced DNA synthesis in T cells but function poorly as antigen-presenting cells compared to pulmonary macrophages.
abstract: This study was undertaken to investigate the ability of bronchial epithelial cells (ECs) to function as accessory cells. Pulmonary monocytes were our reference cells. ECs and pulmonary monocytes were isolated from the bronchial mucosa and pulmonary parenchyma of subjects undergoing lobectomy for standard clinical reasons. Circulating autologous T cells were rigorously depleted of accessory cells to the extent that they lost the capacity to respond to mitogenic lectins alone. ECs restored mitogen-induced DNA synthesis and DNA synthesis triggered by CD3 cross-linking in T cells, as did pulmonary macrophages, and this was unrelated to HLA-DR expression. The ability of promoting T cell proliferation after CD3 cross-linking was, in part, due to the secretory products of ECs, since their supernatants were also effective. Interferon-gamma-treated ECs were capable of presenting antigens to autologous T cells. This was an HLA-DR-restricted phenomenon, but EC efficiency in this system was less than 40% of efficiency demonstrated by pulmonary monocytes. However, ECs greatly upregulated antigen-specific responses supported by pulmonary monocytes.
authors.affiliation: Department of Internal Medicine, University of Milan, School of Medicine, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Mezzetti
authors.forename: M
authors.initials: M
authors.lastname: Soloperto
authors.forename: A
authors.initials: A
authors.lastname: Fasoli
authors.forename: S
authors.initials: S
authors.lastname: Mattoli
mesh_terms: D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000378:metabolism; D015703:Antigens, CD / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D001980:Bronchi / Q000166:cytology / Q000276:immunology*; D017252:CD3 Complex; D002455:Cell Division / Q000276:immunology; D002469:Cell Separation; D002478:Cells, Cultured / Q000276:immunology / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects*; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D008168:Lung / Q000166:cytology / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008934:Mitogens / Q000494:pharmacology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D006684:HLA-DR Antigens; D008934:Mitogens; D011948:Receptors, Antigen, T-Cell; D004247:DNA