title: Detection of HPV DNA in neoplastic and non-neoplastic cervical specimens from Pakistan and Japan by non-isotopic in situ hybridization.
abstract: In order to determine the prevalence and type-specific distribution of human papillomavirus (HPV) in women from Pakistan, a country with a low cervical cancer rate (hospital-based data), and to compare these results with their counter-parts in Japan, we studied 56 non-neoplastic cervical tissues and 162 cervical carcinoma cases from both countries. HPV infection was defined by in situ hybridization in paraffin-embedded tissues using biotinylated HPV 6/11, 16 and 18 probes. The overall positivity rates in non-neoplastic and neoplastic cervical tissues from Pakistan were 33% and 88%, while in those from Japan the rates were 46% and 80%, respectively. High-risk HPVs (16 & 18) were found in 17% of the non-neoplastic specimens and in 69% of cervical carcinoma cases from Pakistan, while Japanese figures in this respect were 19% and 68%, respectively. No correlation was found between the type-specific distribution or prevalence of HPV and the geographic location of the cases examined in the 2 countries. However, in comparison to Japanese women, the incidence of HPV-16-positive cervical carcinoma in Pakistani women decreased significantly (p less than 0.05) in the oldest age group as compared to the youngest age group.
authors.affiliation: Department of Pathology, Fukui Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Anwar
authors.forename: M
authors.initials: M
authors.lastname: Inuzuka
authors.forename: T
authors.initials: T
authors.lastname: Shiraishi
authors.forename: K
authors.initials: K
authors.lastname: Nakakuki
mesh_terms: D000230:Adenocarcinoma / Q000382:microbiology; D000328:Adult; D000367:Age Factors; D002278:Carcinoma in Situ / Q000382:microbiology; D002294:Carcinoma, Squamous Cell / Q000382:microbiology; D002584:Cervix Uteri / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007564:Japan; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D010154:Pakistan; D027383:Papillomaviridae / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
chemical_list: D004279:DNA, Viral