title: Evidence that unphosphorylated smooth muscle myosin supports smooth muscle contraction.
abstract: Unphosphorylated smooth muscle myosin filaments do not disassemble in MgATP, provided that the solution is supplemented either by 25% serum albumin or by 6% polyethylene glycol 6000. These filaments are able to support actomyosin retraction but their ATPase activity is not activated by tropomyosin-decorated F-actin.
authors.affiliation: Istituto di Chimica Biologica, Università di Ferrara, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Grazi
authors.forename: G
authors.initials: G
authors.lastname: Trombetta
mesh_terms: D000199:Actins / Q000502:physiology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002645:Chickens; D005895:Gizzard, Avian / Q000502:physiology; D008854:Microscopy, Electron; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000502:physiology*; D009132:Muscles / Q000502:physiology*; D009218:Myosins / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D011092:Polyethylene Glycols; D011817:Rabbits; D012710:Serum Albumin, Bovine; D014335:Tropomyosin / Q000502:physiology
chemical_list: D000199:Actins; D014335:Tropomyosin; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D000255:Adenosine Triphosphate; D009218:Myosins