Microdose vasal injection of sodium fluoride in the rat.
A single microdose (50 micrograms/50 microL) injection of sodium fluoride (NaF) into the vasa deferentia of adult male albino rats (Rattus norvegicus) caused arrest of spermatogenesis and absence of spermatozoa in the lumina of the seminiferous tubules of the testes, which consequently led to a decline in the sperm count in the caudae epididymides. Scanning electron microscopy of cauda and vas deferens sperm revealed deflagellation and tail abnormalities. This is probably related to the alterations in the internal milieu of these organs which rendered the spermatozoa immotile and consequently caused fertility impairment in the experimental animals. Thus microdoses of sodium fluoride were found to affect reproductive function and fertility rate.
Chinoy
N J
NJ
Department of Zoology, University School of Sciences, Gujarat University, Ahamadabad, India.
Rao
M V
MV
Narayana
M V
MV
Neelakanta
E
E
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002784:Cholesterol / Q000097:blood; D004822:Epididymis / Q000187:drug effects / Q000648:ultrastructure; D005298:Fertility / Q000187:drug effects; D005632:Fructose / Q000378:metabolism; D006651:Histocytochemistry; D008297:Male; D008845:Microinjections; D008855:Microscopy, Electron, Scanning; D051381:Rats; D012098:Reproduction / Q000187:drug effects; D012969:Sodium Fluoride / Q000008:administration & dosage / Q000633:toxicity*; D013076:Sperm Count / Q000187:drug effects; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000648:ultrastructure; D013737:Testis / Q000187:drug effects / Q000648:ultrastructure; D014649:Vas Deferens / Q000187:drug effects / Q000648:ultrastructure
D005632:Fructose; D012969:Sodium Fluoride; D002784:Cholesterol; D000251:Adenosine Triphosphatases