Biological characterisation of Australian isolates of chicken anaemia agent.
Three Australian isolates of chicken anaemia agent (CAA) resisted treatment at 70 degrees C for 5 min and chloroform treatment. Although minor antigenic differences were detected using monoclonal antibodies to CAA, the Australian isolates were indistinguishable from the reference Cux-1 and Gifu-1 isolates in cross-immunofluorescence and cross-neutralisation tests employing polyclonal chicken antiserums. The Australian viruses were pathogenic for intramuscularly inoculated 1-day-old SPF chicks, but were less pathogenic for 7-day-old chicks. Thus the Australian isolates of CAA did not differ significantly in these properties from previously characterised CAA isolates from other continents.
Connor
T J
TJ
Veterinary Research Laboratories, Stormont, Belfast, Northern Ireland.
McNeilly
F
F
Firth
G A
GA
McNulty
M S
MS
D000740:Anemia / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis; D001315:Australia; D002460:Cell Line; D002645:Chickens; D003588:Cytopathogenic Effect, Viral; D004267:DNA Viruses / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D009500:Neutralization Tests; D011201:Poultry Diseases / Q000382:microbiology*; D013047:Specific Pathogen-Free Organisms
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral