Immunosuppressive factor in cow follicular fluid.
The activity of the immunosuppressive fraction and proteinase inhibitor isolated from cow follicular fluid was investigated. The immunosuppressive factor was separated from the accompanying proteinase inhibitor by Sepharose 6B and Sephacryl S-200 column chromatography. In vitro, the fraction significantly reduced mitogen-induced lymphocyte proliferation. In vivo, the fraction inhibited mouse plaque formation. However, the factor had no effect on the development of intact or zona free embryos. The active components have a molecular mass of about 110,000.
Veselský
L
L
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
Dostál
J
J
Landa
V
V
Stanêk
R
R
Vinter
P
P
D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D002417:Cattle / Q000276:immunology* / Q000502:physiology; D002850:Chromatography, Gel; D004031:Diestrus; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005260:Female; D007108:Immune Tolerance; D008213:Lymphocyte Activation / Q000187:drug effects; D016131:Lymphocyte Subsets / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C / Q000196:embryology; D008970:Molecular Weight; D006080:Ovarian Follicle / Q000502:physiology*; D011480:Protease Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology
D011480:Protease Inhibitors