Sequence analysis of a cDNA encoding a group 3 LEA mRNA inducible by ABA or dehydration stress in wheat.
A cDNA clone (pMA2005) of a Group 3 LEA (late embryogenesis abundant) protein has been sequenced from wheat. The wheat cDNA clone codes for a protein with ten tandem repeats of an 11 amino acid sequence and has homology to other Group 3 LEAs reported in barley, carrot, cotton and rape (L. Dure et al., Plant Mol Biol 12: 475-486, 1989). The deduced amino acid sequence indicates that the wheat protein has a molecular weight of 23,000 and is a basic, hydrophilic protein. Northern analysis with the cDNA clone shows that dehydration of wheat shoot tissue results in increased transcript levels that correlate with increases in endogenous ABA.
Curry
J
J
U.S. Department of Agriculture, Washington State University, Pullman 99164-6420.
Morris
C F
CF
Walker-Simmons
M K
MK
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003890:Desiccation; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000502:physiology; D014908:Triticum / Q000187:drug effects / Q000196:embryology / Q000235:genetics*
D010940:Plant Proteins; C066246:late embryogenesis abundant protein, plant; D000040:Abscisic Acid