Pathogenicity and antigenicity of avian nephritis isolates.
The G-4260, IR-N, M-6, and M-8 strains of avian nephritis virus (ANV) were inoculated orally into 1-day-old specific-pathogen-free chicks of the line PDL-1 for pathological and serological study. Five of 15 chicks inoculated with the G-4260 strain died with visceral urate depositions. One of 15 chicks each inoculated with the M-6 and M-8 strains died with nephrosis and visceral urate deposition, respectively. No chicks inoculated with the IR-N strain died. Mean body weights of ANV-inoculated chicks, except for the IR-N-inoculated chicks, at 14 days postinoculation (PI) were significantly lower than those of control chicks (P less than 0.01). However, interstitial nephritis was observed in all ANV-inoculated birds that were histopathologically examined at 14 days PI. In the serological study, the G-4260 and IR-N strains were classified as the same serotype, and the M-6 and M-8 strains were classified as a different serotype from the G-4260 and IR-N strains. These results indicate that there at least two serotypes of ANV and its strains differ in pathogenicity.
Shirai
J
J
Poultry Disease Laboratory, National Institute of Animal Health, Gifu, Japan.
Nakamura
K
K
Shinohara
K
K
Kawamura
H
H
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001835:Body Weight; D002645:Chickens; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D007668:Kidney / Q000382:microbiology / Q000473:pathology; D009500:Neutralization Tests; D010849:Picornaviridae / Q000276:immunology / Q000472:pathogenicity*; D010850:Picornaviridae Infections / Q000382:microbiology / Q000662:veterinary*; D011201:Poultry Diseases / Q000382:microbiology*; D013047:Specific Pathogen-Free Organisms
D000956:Antigens, Viral