title: Continuous-flow synthesis of alpha-gliadin peptides in an ultrasonic field and assay of their inhibition of intestinal sucrase activity.
abstract: Three dodecapeptide amides derived from the amino acid sequence of alpha-gliadin and four of their analogues were synthesized by continuous-flow solid-phase multiple peptide synthesis. Ultrasonic field conditions accelerated the coupling reaction without affecting purity. Biological tests of the synthetic fragments showed the relevance of toxicity prediction for preselection of immunogenic alpha-gliadin fragments.
authors.affiliation: Research Institute for Feed Supplements and Veterinary Drugs, Czechoslovakia.
authors.forename: J
authors.initials: J
authors.lastname: Vágner
authors.forename: P
authors.initials: P
authors.lastname: Kocna
authors.forename: V
authors.initials: V
authors.lastname: Krchnák
mesh_terms: D017456:Adenovirus Early Proteins; D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo; D004386:Duodenum / Q000187:drug effects / Q000201:enzymology; D004723:Endorphins / Q000737:chemistry; D005903:Gliadin / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000737:chemistry; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D011487:Protein Conformation; D013393:Sucrase / Q000037:antagonists & inhibitors; D014465:Ultrasonics
chemical_list: D017456:Adenovirus Early Proteins; D004723:Endorphins; D009856:Oncogene Proteins, Viral; D010446:Peptide Fragments; C021968:beta-casomorphins; D005903:Gliadin; D013393:Sucrase