Detection and localization of the i protein in Escherichia coli cells using antibodies.
Using antibodies raised against the purified i protein, the expression of the chromosomal uncI gene was demonstrated. The i protein was identified as a component of the cytoplasmic membrane and shown to be present in preparations of Fo or F1Fo. The protein is not associated with the F1 moiety.
Schneppe
B
B
Universität Osnabrück, Fachbereich Biologie/Chemie, Arbeitsgruppe Mikrobiologie, Germany.
Deckers-Hebestreit
G
G
Altendorf
K
K
D000906:Antibodies; D001426:Bacterial Proteins / Q000276:immunology / Q000378:metabolism*; D015153:Blotting, Western; D002876:Chromosomes, Bacterial; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D008565:Membrane Proteins / Q000378:metabolism; D010957:Plasmids; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism
D000906:Antibodies; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C071886:i protein, E coli; D006180:Proton-Translocating ATPases