title: A new specific DNA endonuclease activity in yeast mitochondria.
abstract: Two group I intron-encoded proteins from the yeast mitochondrial genome have already been shown to have a specific DNA endonuclease activity. This activity mediates intron insertion by cleaving the DNA sequence corresponding to the splice junction of an intronless strain. We have discovered in mitochondrial extracts from the yeast strain 777-3A a new DNA endonuclease activity which cleaves the fused exon A3-exon A4 junction sequence of the CO XI gene.
authors.affiliation: Laboratoire de Génétique Moléculaire CNRS UA 1302, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Sargueil
authors.forename: A
authors.initials: A
authors.lastname: Delahodde
authors.forename: D
authors.initials: D
authors.lastname: Hatat
authors.forename: G L
authors.initials: GL
authors.lastname: Tian
authors.forename: J
authors.initials: J
authors.lastname: Lazowska
authors.forename: C
authors.initials: C
authors.lastname: Jacq
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D004262:DNA Restriction Enzymes / Q000235:genetics* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D004706:Endodeoxyribonucleases / Q000235:genetics* / Q000378:metabolism; D005091:Exons; D005800:Genes, Fungal; D007438:Introns; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D004706:Endodeoxyribonucleases; C067893:AI3 protein, S cerevisiae; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific