[Protection of human peripheral blood leukocytes with vitamin E and C from toxic effects of fenarimol in vitro].
The work was undertaken in a trial to protect leukocytes against the toxic effect of fenarimol with vitamin E and C. The experiment was carried out in vitro. These vitamins were chosen since they are the components of the physiological antioxidative mechanism in the cell. The protective effect of the vitamins varied depending on the type of the cell and the studied structure as well as on the dose of the antioxidant used. Improvement of the phagocytic activity of the granulocytes was observed after preincubation with vitamin E and using vitamin C it was possible to achieve control values of the phagocytosis index and per cent of non-phagocytizing granulocytes. Both these vitamins in a satisfactory degree improved the morphological and quantitative pattern of lysosomes in cultured lymphocytes treated with fenarimol. The use of vitamin E in doses of 15 micrograms/ml and vitamin C - 0.5 microgram/ml, made possible achieving of complete normalization of the nucleologram in the lymphocytes. The observed effective protection of the leukocytes against the effects of fenarimol by means of antioxidants suggests that these cells are damaged by the mechanism of lipid peroxidation.
Grabarczyk
M
M
Zakładu Fizjopatologii Instytutu Hematologii, Warszawie.
Podstawka
U
U
Kopeć-Szlezak
J
J
D000975:Antioxidants; D001205:Ascorbic Acid / Q000494:pharmacology*; D003470:Culture Media; D005659:Fungicides, Industrial / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000502:physiology; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects* / Q000276:immunology; D011743:Pyrimidines / Q000037:antagonists & inhibitors / Q000633:toxicity*; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D003470:Culture Media; D005659:Fungicides, Industrial; D011743:Pyrimidines; D014810:Vitamin E; C033789:fenarimol; D001205:Ascorbic Acid
Osłanianie leukocytów ludzkiej krwi obwodowej za pomoca witamin E i C przed skutkami toksycznego działania fenarimolu in vitro.