title: Phosphatase production by a Citrobacter sp. growing in batch cultures retarded by anaerobic or osmotic stress, and the effect of the osmoprotectant glycine betaine.
abstract: The effect of 500 mM NaCl on the growth, and phosphatase production of a Citrobacter sp. was investigated. Although growth was retarded, phosphatase production was enhanced by 50%. Relief from osmotic stress using the osmoprotectant glycine betaine gave normal growth, but phosphatase activity was reduced. The Citrobacter sp. ceased to grow following a shift to anaerobic conditions, but anaerobically-incubated cells continued to produce phosphatase after a transient lag.
authors.affiliation: Department of Biochemistry, University of Oxford, U.K.
authors.forename: D S
authors.initials: DS
authors.lastname: Hallett
authors.forename: P
authors.initials: P
authors.lastname: Clark
authors.forename: L E
authors.initials: LE
authors.lastname: Macaskie
mesh_terms: D000693:Anaerobiosis; D001622:Betaine / Q000494:pharmacology*; D002954:Citrobacter / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D006706:Homeostasis; D007700:Kinetics; D009997:Osmotic Pressure; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D014882:Water-Electrolyte Balance
chemical_list: D001622:Betaine; D012965:Sodium Chloride; D010744:Phosphoric Monoester Hydrolases