title: [Evaluation of an immunoenzyme technique (ELISA) to diagnose typhoid fever].
abstract: The efficiency of an ELISA method, designed to detect polyvalent IgG and IgM antibodies to Salmonella typhi polysaccharide was evaluated in patients admitted or convalescing from typhoid fever and in control subjects. Polyvalent antibodies to S typhi were demonstrated in 28/30 (93%) typhoid patients, 0/11 bacteremic patients (E coli or S paratyphi A) and 0/15 asymptomatic individuals. Widal test showed significant anti-0 agglutinin values (> = 1: 160) in only 12/30 (40%), 1/11 (9%) and 0/15 subjects from each group respectively. Typhoid patients tested on admission or at discharge showed similar high reactivity rates to ELISA. On the contrary, the Widal test detected only 2/15 (13%) patients on admission (p < 0.02) and 10/15 (67%) at discharge. These results and additional immunoblotting tests suggest that ELISA developed to detect polyvalent anti-LPS antibodies could represent a highly specific diagnostic tool to confirm typhoid fever in endemic areas.
authors.affiliation: Unidad de Microbiología, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Santiago de Chile.
authors.forename: G
authors.initials: G
authors.lastname: Figueroa
authors.forename: M
authors.initials: M
authors.lastname: Jashés
authors.forename: G
authors.initials: G
authors.lastname: Fáundez
authors.forename: M S
authors.initials: MS
authors.lastname: Toledo
authors.forename: M
authors.initials: M
authors.lastname: Troncoso
authors.forename: L
authors.initials: L
authors.lastname: Aguad
mesh_terms: D000328:Adult; D002677:Chile; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008070:Lipopolysaccharides; D012485:Salmonella typhi / Q000276:immunology; D014435:Typhoid Fever / Q000097:blood / Q000175:diagnosis*
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides
vernacular_title: Evaluación de un ensayo inmunoenzimático (ELISA) para el diagnóstico de la fiebre tifoídea.