Alpha beta T-lymphocyte depleted mice, a model for gamma delta T-lymphocyte functional studies.
Adult mice can be depleted of essentially all mature alpha beta T lymphocytes by chronic treatment with the framework-recognizing, pan-specific anti-TCR alpha beta mAb, H57-597. Similar findings have been reported in rats, gamma delta cell populations remain essentially unaltered in size and reactivity. Suppression of alpha beta T-cell development results in the loss of alloantigen reactivity and of B-cell help, suggesting that gamma delta and alpha beta populations differ in their functional capabilities. Indirect effects of the antibody treatment include quantitative changes in splenic B cells, as well as reduced sizes and weights of experimental animals. alpha beta-suppressed mice and rats may provide model systems for studies on gamma delta cell function in vivo.
Carbone
A
A
Department of Molecular and Cellular Biology, University of California, Berkeley.
Harbeck
R
R
Dallas
A
A
Nemazee
D
D
Finkel
T
T
O'Brien
R
R
Kubo
R
R
Born
W
W
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002469:Cell Separation; D006224:Cricetinae; D005434:Flow Cytometry; D008211:Lymphocyte Cooperation; D008212:Lymphocyte Depletion; D051379:Mice; D051381:Rats; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000254:growth & development
D000911:Antibodies, Monoclonal; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta