title: Analysis of primary T cell responses to intact and fractionated microbial pathogens.
abstract: Freshly isolated human T lymphocytes were tested for their response to mycobacteria, mycobacterial lysates, 2 dimensional (2D) PAGE separated mycobacterial lysates, leishmania and defined leishmanial antigen preparations. While gamma delta T cells proliferated vigorously in the presence of mycobacteria and mycobacteria derived lysates, a significant stimulation from 2 D gel separated lysates was not detected. In addition gamma delta T cells failed to respond towards leishmania or leishmanial components. In the alpha beta T cell compartment some donors, presumably according to their state of immunity against mycobacteria, responded to mycobacteria, mycobacterial lysates and 2 D gel separated mycobacterial lysates. Neither freshly isolated gamma delta T cells nor alpha beta T cells from naive donors did mount a significant immune response against leishmania.
authors.affiliation: Institute of Medical Microbiology and Hygiene, Technical University of Munich, FRG.
authors.forename: K
authors.initials: K
authors.lastname: Pfeffer
authors.forename: B
authors.initials: B
authors.lastname: Schoel
authors.forename: H
authors.initials: H
authors.lastname: Gulle
authors.forename: H
authors.initials: H
authors.lastname: Moll
authors.forename: S
authors.initials: S
authors.lastname: Kromer
authors.forename: S H
authors.initials: SH
authors.lastname: Kaufmann
authors.forename: H
authors.initials: H
authors.lastname: Wagner
mesh_terms: D000818:Animals; D006360:Heat-Shock Proteins / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D007891:Leishmania / Q000276:immunology; D008213:Lymphocyte Activation; D009161:Mycobacterium / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D006360:Heat-Shock Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta