Tn5-rpsL: a new derivative of transposon Tn5 useful in plasmid curing.
The rpsL gene of Escherichia coli was inserted into the BamHI site of transposon Tn5. This transposon was called Tn5-rpsL. Tn5-rpsL may be useful in microbiological studies when one wants to cure various bacterial genera of certain plasmid(s). A streptomycin-resistant (SmR) derivative of the host bacterial strain is first isolated. The plasmid(s) later to be cured are then labelled with Tn5-rpsL, which makes the cells Sm-sensitive. These cells can regain their resistance to Sm if they lose the Tn5-rpsL-tagged plasmid. Thus, plasmid-free bacteria are easily selected among SmR survivors. The frequency of occurrence of the plasmid-less variants of plasmid-containing wild-type Salmonella typhimurium measured by this method is given as an example.
Stojiljković
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Ruder Bosković Institute, Zagreb, Croatia Yugoslavia.
Trgovcević
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Salaj-Smic
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D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005818:Genetic Engineering / Q000379:methods; D010957:Plasmids; D015183:Restriction Mapping; D000091243:Ribosomal Protein S9; D012486:Salmonella typhimurium / Q000235:genetics
D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D000091243:Ribosomal Protein S9; C000717190:RpsI protein, E coli