Inhibition of DNA chain elongation by a phosphotriester linkage in template oligodeoxyribonucleotides.
We synthesized two oligodeoxyribonucleotides bearing an isopropyl phosphotriester at defined positions using new phosphorobisamidite chemistry. Diastereomers were separated with HPLC and their template properties were analyzed. Priming oligodeoxyribonucleotides labeled with 32P at the 5'-end were annealed to the modified oligodeoxyribonucleotides and elongated with DNA polymerase I large fragment from Escherichia coli. Results show that the phosphotriester inhibits the DNA chain elongation partially and the extents of the inhibition are remarkably different between the two diastereomers.
Yashiki
T
T
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Yamana
K
K
Nishijima
Y
Y
Negishi
K
K
D001483:Base Sequence; D004247:DNA / Q000096:biosynthesis*; D004256:DNA Polymerase I / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D004952:Esters / Q000737:chemistry; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000378:metabolism*; D013698:Templates, Genetic
D004952:Esters; D009838:Oligodeoxyribonucleotides; D004247:DNA; D004256:DNA Polymerase I