title: A stable isotope dilution mass spectrometric assay for the major urinary metabolite of PGD2.
abstract: 1. A sensitive and specific negative ion chemical ionization mass spectrometric assay for the major urinary metabolite of PGD2 has been developed employing a chemically synthesized [18(0)4]-labelled internal standard. 2. The finding that increased urinary excretion of this metabolite occurs in a number of clinical situations suggests that the assay may prove to be a valuable tool to explore the role of PGD2 in the pathophysiology of human disease.
authors.affiliation: Department of Pharmacology, Vanderbilt University, Nashville, TN 37232-6602.
authors.forename: J D
authors.initials: JD
authors.lastname: Morrow
authors.forename: C
authors.initials: C
authors.lastname: Prakash
authors.forename: T A
authors.initials: TA
authors.lastname: Duckworth
authors.forename: W E
authors.initials: WE
authors.lastname: Zackert
authors.forename: I A
authors.initials: IA
authors.lastname: Blair
authors.forename: J A
authors.initials: JA
authors.lastname: Oates
authors.forename: L J
authors.initials: LJ
authors.lastname: Roberts
mesh_terms: D001249:Asthma / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D006632:Histamine / Q000378:metabolism; D006801:Humans; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism; D006937:Hypercholesterolemia / Q000188:drug therapy / Q000652:urine; D007201:Indicator Dilution Techniques; D013058:Mass Spectrometry / Q000379:methods*; D008415:Mastocytosis / Q000652:urine; D009525:Niacin / Q000494:pharmacology / Q000627:therapeutic use; D015230:Prostaglandin D2 / Q000378:metabolism*; D011457:Prostaglandins D / Q000032:analysis* / Q000096:biosynthesis
chemical_list: D011457:Prostaglandins D; D009525:Niacin; C035860:9-hydroxy-11,15-dioxo-2,3,18,19-tetranorprost-5-ene-1,20-dioic acid; D006632:Histamine; D006911:Hydroxyprostaglandin Dehydrogenases; C031051:prostaglandin-F synthase; D015230:Prostaglandin D2