Activation-associated marker proteins: peptide mapping and their expression in macrophage cell lines.
The activation-associated markers, p47b and p71/73, have been recognized as minor proteins in peritoneal and bone marrow culture-derived macrophages activated for tumor cell killing. Proteins with identical characteristics of migration on 2-dimensional gels and comparable Cleveland peptide maps are described here in macrophage cell lines that could be activated for tumor cell killing (J774A.1, RAW 264, UNC-2). Macrophage cell lines that could not be activated (P388D1 and PU5-1.8) did not express the markers. The expression of these markers in activatable macrophage cell lines strengthens their association with the activation process and provides a bulk source of the proteins for purification studies.
Pinson
D M
DM
Wilkinson Laboratory for Cancer Research, University of Kansas Cancer Center, Kansas City.
Phillips
T A
TA
Pace
J L
JL
MacKay
R J
RJ
Russell
S W
SW
D000818:Animals; D002460:Cell Line; D002373:Cyclic AMP Receptor Protein / Q000235:genetics*; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D010449:Peptide Mapping; D011506:Proteins / Q000276:immunology*
D002373:Cyclic AMP Receptor Protein; D011506:Proteins