Identification and characterization of sex-linked proteins of Schistosoma mansoni.
The proteins of adults worms (male and female) of two isolates (BH and RJ) of Schistosoma mansoni were extracted using Triton X-114 phase separation. The SDS-polyacrilamide gel electrophoresis profiles of the three phases (detergent, aqueous and insoluble proteins) obtained were compared after Coomassie blue and silver staining, surface radioiodination and Western blotting. No major differences were detected between the 2 isolates. Of the 25 or more proteins which partitioned into the detergent phase, only about 8 proteins could be surface radiodinated on live adult worms. A comparison was also made between the profiles of male and females worms, isolated from bisexually infected mice. Two major female-specific and one male-specific band were detected by silver and/or Coomassie staining. The female bands, 32 KDa and 18 KDa, partitioned into the detergent and aqueous phase, respectively. The male-specific band of 42 KDa remained in the insoluble phase. Antigenic differences between male and females proteins were detected by Western blotting using a sera from infected Nectomys squamipes.
Maldonado Júnior
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Instituto Oswaldo Cruz, Departamento de Biologia, Rio de Janeiro, Brasil.
de Simone
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SG
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D015153:Blotting, Western; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D017830:Octoxynol; D011092:Polyethylene Glycols; D012550:Schistosoma mansoni / Q000737:chemistry*; D012737:Sex Factors
D000954:Antigens, Surface; D003902:Detergents; D008565:Membrane Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40