GM-1, a clone of the monoblastic phagocyte U937 that expresses a large respiratory burst capacity upon activation with interferon-gamma.
The human cell line U937 was cloned and screened for the responsiveness to interferon-gamma (INF-gamma). The selected subclone, named GM-1, expressed a high density of IFN-gamma receptors and showed HLA typing similar to that of the parental line but was devoid of the Y chromosome. GM-1 cells display a promyeloid phenotype as revealed by flow cytometry using a panel of murine antibodies. Following treatment with IFN-gamma GM-1 cells differentiated to a more mature monocyte stage and acquired the capacity to mount a respiratory burst. After treatment with differentiation promotors, such as phorbol 12-myristate 13-acetate (PMA), dimethyl sulfoxide (DMSO), and retinoic acid, GM-1 showed a more limited respiratory burst capacity. Superoxide release in IFN-gamma-activated cells was stimulated with f-Met-Leu-Phe, C5a, or PMA. The development of the respiratory burst capacity was accompanied with the expression of cytochrome b558, a component of the phagocyte NADPH-oxidase. GM-1 cells are useful for the study of the effects of IFN-gamma on the respiratory burst. They are more sensitive and yield a more homogenous response to IFN-gamma than U937 cells. The phenotype of GM-1 cells was stable for more than 5 years.
Garotta
G
G
Central Research Units, F. Hoffmann-La Roche & Co. Ltd., Basel, Switzerland.
Thelen
M
M
Delia
D
D
Kamber
M
M
Baggiolini
M
M
D015703:Antigens, CD / Q000032:analysis; D003573:Cytochrome b Group / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007111:Immunity, Cellular / Q000187:drug effects; D007371:Interferon-gamma / Q000494:pharmacology*; D008262:Macrophage Activation; D008264:Macrophages / Q000166:cytology*; D009000:Monocytes / Q000166:cytology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D019255:NADPH Oxidases; D011994:Recombinant Proteins; D013057:Spectrum Analysis; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D003573:Cytochrome b Group; D011994:Recombinant Proteins; D013481:Superoxides; D014212:Tretinoin; D009240:N-Formylmethionine Leucyl-Phenylalanine; D007371:Interferon-gamma; C036888:cytochrome b558; D006861:Hydrogen Peroxide; D019255:NADPH Oxidases; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide