Effect of cadmium on Ca2+ transport in brain microsomes.
The effect of Cd2+ on Ca2+ transport properties (uptake/release) in rat brain microsomes is examined by the tracer method using 45Ca2+. Cadmium ion (Cd2+) shows a dose-dependent inhibition of Ca(2+)-ATPase activity and consequently, exhibits a reduction in ATP-dependent Ca2+ uptake. In addition to this, Cd2+ also stimulates a rapid release of Ca2+ (t1/2 = 0.5 min) from the microsomes in a dose-dependent manner. The effect of Cd2+ is reversible by 1 mM cysteine or dithiothreitol (DTT). It is suggested that Cd2+ plays an important role in regulating the transmembrane flux of the cations in the microsomes. This effect is dramatically modulated by DTT suggesting a role of sulfhydryl groups in Ca(2+)-transport.
Shah
J
J
Department of Biaphysics, Boston University School of Medicine, MA 02118.
Pant
H C
HC
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001921:Brain / Q000378:metabolism*; D002104:Cadmium / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D003545:Cysteine / Q000494:pharmacology; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D002104:Cadmium; D000252:Calcium-Transporting ATPases; D008274:Magnesium; D003545:Cysteine; D002118:Calcium