title: Nucleotide sequence of cDNA coding for dianthin 30, a ribosome inactivating protein from Dianthus caryophyllus.
abstract: Rabbit antibodies raised against dianthin 30, a ribosome inactivating protein from carnation (Dianthus caryophyllus) leaves, were used to identify a full length dianthin precursor cDNA clone from a lambda gt11 expression library. N-terminal amino acid sequencing of purified dianthin 30 and dianthin 32 confirmed that the clone encoded dianthin 30. The cDNA was 1153 basepairs in length and encoded a precursor protein of 293 amino acid residues. The first 23 N-terminal amino acids of the precursor represented the signal sequence. The protein contained a carboxy-terminal region which, by analogy with barley lectin, may contain a vacuolar targeting signal.
authors.affiliation: Department of Biological Sciences, University of Warwick, Coventry, U.K.
authors.forename: G
authors.initials: G
authors.lastname: Legname
authors.forename: P
authors.initials: P
authors.lastname: Bellosta
authors.forename: G
authors.initials: G
authors.lastname: Gromo
authors.forename: D
authors.initials: D
authors.lastname: Modena
authors.forename: J N
authors.initials: JN
authors.lastname: Keen
authors.forename: L M
authors.initials: LM
authors.lastname: Roberts
authors.forename: J M
authors.initials: JM
authors.lastname: Lord
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D000922:Immunotoxins; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases; D037121:Plant Lectins; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics*; D015183:Restriction Mapping; D054789:Ribosome Inactivating Proteins, Type 1; D012276:Ricin / Q000737:chemistry; D000076985:Saporins; D016415:Sequence Alignment; D015978:Trichosanthin / Q000737:chemistry
chemical_list: D000922:Immunotoxins; D037121:Plant Lectins; D010940:Plant Proteins; D021382:Protein Sorting Signals; D054789:Ribosome Inactivating Proteins, Type 1; D015978:Trichosanthin; D012276:Ricin; D009699:N-Glycosyl Hydrolases; D000076985:Saporins