Rat-liver-type phosphofructokinase mRNA. Structure, tissue distribution and regulation.
We have cloned a full-length cDNA for rat-liver-type phosphofructokinase. The similarities of the rat liver-type phosphofructokinase mRNA to the human and mouse counterparts were 94% and 99% in their amino acid sequences and 88% and 94% in the nucleotide sequences of their coding regions, respectively. Rat liver-type phosphofructokinase mRNA was expressed in all tissues examined, but its level was regulated tissue-specifically. The nutritional and hormonal regulations of the mRNA in the liver were examined in comparison with those of two other key glycolytic enzymes, glucokinase and L-type pyruvate kinase. The level of liver-type phosphofructokinase mRNA was essentially unchanged by starvation (72 h) or diabetes. The mRNA level also did not change significantly on refeeding starved rats on a high carbohydrate diet, or treating diabetic ones with insulin. These results suggested that rat liver-type phosphofructokinase mRNA in the liver was not under control of diet or insulin, in contrast to glucokinase and L-type pyruvate kinase.
Hotta
K
K
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Nakajima
H
H
Yamasaki
T
T
Hamaguchi
T
T
Kuwajima
M
M
Noguchi
T
T
Tanaka
T
T
Kono
N
N
Tarui
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D005941:Glucokinase / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000502:physiology; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010732:Phosphofructokinase-1 / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D012689:Sequence Homology, Nucleic Acid
D007328:Insulin; D012333:RNA, Messenger; D004247:DNA; D010732:Phosphofructokinase-1; D005941:Glucokinase