The sequence of 8.8 kb of yeast chromosome III cloned in lambda PM3270 contains an unusual long ORF (YCR601).
We have determined the nucleotide sequence of a segment of chromosome III contained in the right part of the lambda PM3270 clone, for a total of 8824 bp. This sequence contains an unusual long open reading frame, YCR601, of 6501 bp that encodes for a protein of 2167 amino acids that show no homology with other known proteins. YCR601 was disrupted by internal deletion and insertion of LEU2 gene and is a non-essential gene, however, it is transcribed during vegetative growth yielding a polyadenylated mRNA of approximately 7 kb.
Rodriguez
F
F
Dipartimento di Fisiologia e Biochimica Generali, Università di Milano, Italy.
Martegani
E
E
Mauri
I
I
Alberghina
L
L
D000595:Amino Acid Sequence; D010582:Bacteriophage lambda; D001483:Base Sequence; D015825:Chromosomes, Fungal / Q000737:chemistry*; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D016385:TATA Box
D004271:DNA, Fungal