Phenotypic analysis of CD23+ peripheral blood mononuclear cells in atopic dermatitis.
There is an increase in the number of CD23+ cells in peripheral blood mononuclear cells (PBMC) in atopic dermatitis (AD). We analysed the subpopulation of CD23+ PBMC in 11 patients with AD and in 10 healthy controls and found that B cells (CD20+) and non-T, non-B cells (CD3- CD20-) (mainly monocytes) were responsible for the elevation of CD23+ cells. CD23+ T cells (CD3+) comprised only 4.6% of total CD23+ cells in AD. The percentage of CD23+ cells did not correlate with the serum log IgE level nor with clinical severity of AD. Interleukin 4 (IL-4) induced the expression of CD23 antigen in PBMC both in AD and in healthy controls in a dose-dependent manner in vitro. This enhancing effect of IL-4 was completely abrogated by the addition of anti-IL-4 monoclonal antibody. Other cytokines such as IL-1, IL-2, IL-3, IFN-alpha, IFN-gamma and TNF-alpha had no significant effects on CD23 expression.
Nakamura
K
K
Department of Dermatology, University of Tokyo, Japan.
Okubo
Y
Y
Minami
M
M
Furue
M
M
Ishibashi
Y
Y
D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000187:drug effects / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000096:biosynthesis / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D002648:Child; D016207:Cytokines / Q000494:pharmacology; D003876:Dermatitis, Atopic / Q000276:immunology*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D015847:Interleukin-4 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011961:Receptors, Fc / Q000096:biosynthesis / Q000187:drug effects / Q000276:immunology*; D017455:Receptors, IgE
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D016207:Cytokines; D011961:Receptors, Fc; D017455:Receptors, IgE; D015847:Interleukin-4; D007073:Immunoglobulin E