Dose-response bioactivity and bioavailability of salmon calcitonin in premenopausal and postmenopausal women.
We investigated the acute, dose-response to three intranasal doses of salmon calcitonin (sCT) (50 IU, 100 IU, and 200 IU) and one im dose (50 IU) in eight premenopausal and eight early postmenopausal women. Total serum calcium and serum beta-endorphin revealed significant changes after all four administrations (P less than 0.05). After the two highest intranasal and the im doses cAMP increased 10% and 35%, respectively (P less than 0.05). All administrations except the 50 IU intranasal dose produced significant increases in plasma sCT (P less than 0.05). The areas under the concentration-time curves, calculated for the period with the maximal changes (i.e. 120-240 min), illustrated a significant dose-related response in total serum calcium, beta-endorphin, and sCT (P less than 0.01-0.001). cAMP showed a dose-related tendency, the response to the im injection being significantly higher than that to the two lowest doses of intranasal sCT (P less than 0.05). We conclude that the doses administered produce a dose-related biological response and bioavailability. In women with normal and high bone turnover, sCT 100 IU intranasally seems as optimal as 50 IU im. The response to sCT should, furthermore, be assessed on bioactivity rather than on bioavailability.
Overgaard
K
K
Department of Clinical Chemistry, Glostrup Hospital, University of Copenhagen, Denmark.
Agnusdei
D
D
Hansen
M A
MA
Maioli
E
E
Christiansen
C
C
Gennari
C
C
D000281:Administration, Intranasal; D000328:Adult; D001682:Biological Availability; D002116:Calcitonin / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D002118:Calcium / Q000097:blood; D000242:Cyclic AMP / Q000097:blood; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007273:Injections, Intramuscular; D008593:Menopause / Q000378:metabolism*; D008875:Middle Aged; D001615:beta-Endorphin / Q000097:blood
D001615:beta-Endorphin; C028815:salmon calcitonin; D002116:Calcitonin; D000242:Cyclic AMP; D002118:Calcium