[Localization of camphor degradative plasmids on the chromosome of Pseudomonas putida strains PaW].
Camphor degradative plasmids (CAM, pRK1) are preferentially situated on chromosomes of Pseudomonas putida strains PaW. After having been transferred into Cam+ strains, the TOL plasmid pWWO dissociates into the cryptic plasmid pWWO-8 and chromosome-borne transposon Tn4651. The opposite situation, i.e. reconstruction of the TOL plasmid pWWO from the cryptic plasmid pWWO-8 and chromosome-borne catabolic operons of the pWWO plasmid has been described. Cam- derivatives of the CAM plasmid were obtained in vivo which contain the TOL plasmid transposons Tn4651 or Tn4652 as obligatory structural elements. These plasmids as well as pWWO-8 determine conjugational mobilization of chromosome-located cam operons followed by their integration into the chromosome of recipient.
Miaé
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AA
Tartu University, Estonian Biocenter.
Kheĭnaru
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AL
D002164:Camphor / Q000378:metabolism*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D010957:Plasmids / Q000235:genetics*; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism
D002164:Camphor
Integrirovannye v khromosomu plazmidy biodegradatsii kamfory shtammov Pseudomonas putida linii PaW.