Fibroblastlike primary cells from human colon adenocarcinoma explants: collagen biosynthesis.
Fibroblastlike primary cells have been obtained from human colon adenocarcinoma explants. Such cells disappear during cell culture and thus have not been previously studied. These cells have a number of altered phenotypic characteristics: a) morphology; b) growth behavior and adherence to culture substrate (they required 3 h for 90% attachment and only presented a flattened morphology 40 h after platting); and c) collagen metabolism. Increased protein biosynthesis (about double than control colon-derived fibroblasts) and maintained ability for collagen biosynthesis have been observed for the tumor-associated fibroblastlike cells. Thus, the collagen to noncollagenous proteins ratio was decreased for these cells. They exhibited an altered type I:type III collagen (5:1 instead of 3:1 in colon fibroblasts) and procollagen (2:1 against 5:1 in colon fibroblasts) ratios as well as a decreased secretion of collagen with an abnormal deposition of procollagens in the cell layer. These studies show a permanent phenotypic alteration in the tumor-associated fibroblast-like cells.
Turnay
J
J
Departamento de Bioquímica y Biologia Molecular, Facultad de Ciencias, Universidad Complutense, Madrid, Spain.
Olmo
N
N
Gavilanes
J G
JG
Lizarbe
M A
MA
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D003094:Collagen / Q000032:analysis / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008875:Middle Aged; D010641:Phenotype; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology
D003094:Collagen