title: Secondary caries related to various marginal gaps around amalgam restorations in vitro.
abstract: Secondary caries lesions were produced in vitro by immersing human tooth-blocks with amalgam restorations every third day for 4 weeks in glucose- or sucrose-containing broth inoculated with a strain of Streptococcus mutans. The lesions at the cavity walls were related to various standardized micro-spaces (0, 30, 40, 60 and 80 microns) around the restorations. When a thick, sucrose induced layer of plaque covered the margin, dentine caries was found in all cases where a gap was present (30 microns or more). In the presence of a thin, glucose induced layer of plaque, dentine caries was detected only in the specimens with 60 and 80 microns gap-widths. Caries lesions were detected on the outer free enamel surfaces in all specimens. Lesions in the enamel of the cavity walls were observed with polarized light in 46% in the presence of sucrose- and in 21% in the presence of glucose-induced plaque.
authors.affiliation: Department of Oral Technology, University of Lund, Malmö.
authors.forename: T
authors.initials: T
authors.lastname: Dérand
authors.forename: D
authors.initials: D
authors.lastname: Birkhed
authors.forename: S
authors.initials: S
authors.lastname: Edwardsson
mesh_terms: D003723:Dental Amalgam / Q000737:chemistry*; D003731:Dental Caries / Q000209:etiology* / Q000382:microbiology / Q000473:pathology; D003743:Dental Enamel / Q000473:pathology; D003763:Dental Leakage / Q000150:complications*; D003773:Dental Plaque / Q000382:microbiology; D003793:Dental Restoration, Permanent / Q000009:adverse effects; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D008852:Microradiography; D008859:Microscopy, Polarization; D012008:Recurrence; D013295:Streptococcus mutans / Q000502:physiology; D013395:Sucrose / Q000494:pharmacology; D013499:Surface Properties
chemical_list: D013395:Sucrose; D003723:Dental Amalgam; D005947:Glucose