EA-1, a novel adhesion molecule involved in the homing of progenitor T lymphocytes to the thymus.
The mouse progenitor T lymphocyte (pro-T) cell line FTF1 binds in vitro to thymus blood vessels, the thymic capsule, and liver from newborn mice. A mAb, EA-1, raised against an embryonic mouse endothelial cell line, blocked adhesion. The antibody also interfered with pro-T cell adhesion to a thymus-derived mouse endothelial cell line; it had no effect on the adhesion of mature T lymphocytes and myeloid cells. The antigen recognized by EA-1 is located on the vascular endothelium of various mouse tissues and absent on pro-T cells. EA-1 antibody precipitates molecules with apparent molecular weights of 110,000, 140,000, 160,000, and 200,000. Immunoclearing and binding-inhibition studies with antibodies against known adhesion molecules suggest that the EA-1 antigen is a novel adhesion molecule involved in colonization of the embryonic thymus by T cell progenitors.
Imhof
B A
BA
Basel Institute for Immunology, Switzerland.
Ruiz
P
P
Hesse
B
B
Palacios
R
R
Dunon
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000276:immunology / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D066298:In Vitro Techniques; D051379:Mice; D008970:Molecular Weight; D011233:Precipitin Tests; D016201:Receptors, Lymphocyte Homing / Q000502:physiology*; D013601:T-Lymphocytes / Q000166:cytology*; D013950:Thymus Gland / Q000166:cytology*; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D016201:Receptors, Lymphocyte Homing