Accumulation of m-iodobenzylguanidine by neuroblastoma cells results from independent uptake and storage mechanisms.
The modalities of uptake and storage of iodine-labeled m-iodobenzylguanidine (MIBG) by four human neuroblastoma cell lines have been studied. SK-N-BE(2)C cell line has been shown to possess the specific (type 1) MIBG uptake, as well as an efficient extravesicular storage mechanism. Conversely, LAN-5 cells, which show a nonsaturation kinetic of MIBG incorporation, lack only the ability to efficiently store the MIBG taken up by a mechanism that can be pharmacologically defined as uptake 1. The two other neuroblastoma cell lines tested, GI-LI-N and GI-CA-N, lack both uptake and storage capacity. In view of the fact that the only detailed study on specific MIBG uptake by a human neuroblastoma cell line has been carried out on SK-N-SH, a highly heterogeneous cell line, our report provides new insights on the molecular and cellular pharmacology of radiolabeled MIBG.
Montaldo
P G
PG
Pediatric Oncology Research Laboratory, G. Gaslini Children's Hospital, Genoa, Italy.
Lanciotti
M
M
Casalaro
A
A
Cornaglia-Ferraris
P
P
Ponzoni
M
M
D019797:3-Iodobenzylguanidine; D000970:Antineoplastic Agents / Q000378:metabolism*; D001692:Biological Transport; D002460:Cell Line; D004734:Energy Metabolism; D006801:Humans; D007462:Iodobenzenes / Q000378:metabolism*; D007700:Kinetics; D009447:Neuroblastoma / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism; D013997:Time Factors
D000970:Antineoplastic Agents; D007462:Iodobenzenes; D019797:3-Iodobenzylguanidine; D010042:Ouabain; D012964:Sodium