title: Reduced levels of mRNA for myofibrillar proteins in skeletal muscle from septic rats.
abstract: The influence of sepsis on transcription of myofibrillar proteins in skeletal muscle was studied in rats. Sepsis was induced by cecal ligation and puncture (CLP); control rats were sham-operated. Sixteen hours later, muscle levels of mRNA for myofibrillar proteins were determined by using cDNA probes specific for transcripts for alpha actin and myosin heavy chain. Sepsis resulted in a 2-6 fold decrease in alpha actin mRNA levels and an even more pronounced reduction in myosin heavy chain mRNA levels. Results suggest that sepsis-induced reduction of muscle protein synthesis is at least partly regulated at the transcriptional level.
authors.affiliation: Department of Surgery University of Cincinnati, Ohio 45267-0558.
authors.forename: P O
authors.initials: PO
authors.lastname: Hasselgren
authors.forename: J V
authors.initials: JV
authors.lastname: Tricoli
authors.forename: D
authors.initials: D
authors.lastname: Wieczorek
authors.forename: K A
authors.initials: KA
authors.lastname: Steigerwald
authors.forename: U
authors.initials: U
authors.lastname: Angerås
authors.forename: M
authors.initials: M
authors.lastname: Hall-Angerås
authors.forename: J E
authors.initials: JE
authors.lastname: Fischer
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D001424:Bacterial Infections / Q000235:genetics*; D015152:Blotting, Northern; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis*; D009218:Myosins / Q000235:genetics; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic
chemical_list: D000199:Actins; D015342:DNA Probes; D009124:Muscle Proteins; D012333:RNA, Messenger; D004247:DNA; D009218:Myosins