Sensitive determination of free and plasma protein-bound dipyridamole by high-performance liquid chromatography.
For many years dipyridamole (DP) has been used in the treatment of hypertension as a vasodilator, but recently it has been recognised as an anti-platelet aggregation agent and to potentiate anti-metabolite activity. A rapid and sensitive (20 nM) procedure for the determination of free and protein-bound DP in plasma, using reversed-phase high-performance liquid chromatography on an Ultrasphere XL ODS (3 microns) column (70 mm x 4.6 mm I.D.) with ultraviolet detection (280 nm), is reported. Free and bound DP were separated using ultrafiltration. Concentrations of DP between 0.1 and 10 microM were measured in plasma with a relative standard deviation of less than 9.6%. The subsequent determination of DP levels in patients orally administered 450 mg per day showed that DP binding to plasma protein is higher than 90%.
Barberi
M
M
Centre Alexis Vautrin, Vandoeuvre-les-Nancy, France.
Merlin
J L
JL
Weber
B
B
D000284:Administration, Oral; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004176:Dipyridamole / Q000008:administration & dosage / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male
D004176:Dipyridamole