title: Subcellular distribution of asialoglycoprotein receptor in liver regeneration.
abstract: Asialoglycoprotein (ASGP) receptor specific activity was determined directly using a radiolabelled ligand (asialoorosomucoid) binding assay in various fractions of rat liver cells before and 24 hours after two-thirds partial hepatectomy. The specific activity of ASGP receptor in the plasma membrane fraction was significantly reduced 24 hours after partial hepatectomy. In an attempt to identify a subgroup of receptor involved in intercellular recognition the calcium-dependent ASGP receptor was re-assayed after pre-incubation in ethylenediaminetetra-acetic acid. This revealed significant amounts of additional receptor in microsomal preparations but not in plasma membrane fractions. Cryptic receptor levels were not affected by partial hepatectomy. Plasma membrane-bound receptor was significantly decreased in regenerating liver.
authors.affiliation: Department of Medicine, University of Cape Town.
authors.forename: D L
authors.initials: DL
authors.lastname: Maeder
authors.forename: E D
authors.initials: ED
authors.lastname: Sturrock
authors.forename: R E
authors.initials: RE
authors.lastname: Kirsch
mesh_terms: D000818:Animals; D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins / Q000378:metabolism*; D008099:Liver / Q000737:chemistry; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D051381:Rats; D011971:Receptors, Immunologic / Q000032:analysis*
chemical_list: D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D011971:Receptors, Immunologic