Anti-IgG antibodies in rheumatic diseases cross-react with Streptococcus mutans SR antigen.
We have previously shown that SR protein, a S. mutans major cell wall protein, as well as the recombinant protein SR (rSR) share common epitopes with human IgG. Since this antigenic mimicry could play a role in the induction of anti IgG, we have examined, in k-ELISA, the presence of antibodies reacting with S. mutans SR proteins and S. mutans whole cells in sera from 36 patients with rheumatic diseases. The majority of the 36 sera showed a high reactivity with rSR when compared with control sera. Eight highly positive sera were further purified on rSR and human IgG sorbents and tested against both rSR and IgG in ELISA and Western blotting. The affinity-purified antibodies reacted strongly with rSR, IgG and IgG Fab fragments but failed to react with IgG Fc fragment. In Western blotting the addition of unlabelled IgG abolished the reactivity of affinity-purified biotinylated antibodies with all antigens, confirming the existence of a common epitope shared by rSR and human IgG heavy chain. We show the existence in rheumatic diseases of high titres of anti-human IgG antibodies cross-reactive with S. mutans SR proteins. Those antibodies are principally IgG and react with the Fd part of the Fab fragment. We can hypothesize from the above data that this antigenic mimicry existing between S. mutans SR-related antigens and human IgG could play a role in the synthesis of at least a part of the anti-IgG antibodies present in rheumatic diseases sera.
Ackermans
F
F
Faculty of Dentistry, National Institute of Health and Medical Research, Strasbourg, France.
Pini
A
A
Wachsmann
D
D
Schöller
M
M
Ogier
J
J
Klein
J P
JP
D000888:Antibodies, Anti-Idiotypic / Q000097:blood / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000276:immunology*; D003429:Cross Reactions; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007158:Immunologic Techniques; D011994:Recombinant Proteins / Q000276:immunology; D012216:Rheumatic Diseases / Q000276:immunology*; D013295:Streptococcus mutans / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins; C063999:SR protein, bacteria