title: Heavy meromyosin induces sliding movements between antiparallel actin filaments.
abstract: Suzuki et al. [Biochemistry 28, 6513-6518 (1989)] have shown that, when F-actin is mixed with inert high polymer, a large number of actin filaments closely align in parallel with overlaps to form a long and thick bundle. The bundle may be designated non-polar, as the constituent filaments are random in polarity (Suzuki et al. 1989). I prepared non-polar bundles of F-actin using methylcellulose (MC) as the high polymer, exposed them to heavy meromyosin (HMM) in the presence of ATP under a light microscope, and followed their morphological changes in the continuous presence of MC. It was found that bundles several tens of micrometers long contracted to about one-third the initial length, while becoming thicker, in half a minute after exposure to HMM. Subsequently, each bundle was split longitudinally into several bundles in a stepwise manner, while the newly formed ones remained associated together at one of the two ends. The product, an aster-like assembly of actin bundles, was morphologically quiescent; that is, individual bundles never contracted upon second exposure to HMM and ATP, although they were still longer than the F-actin used. Bundles in this state consisted of filaments with parallel polarity as examined by electron microscopy. This implies that non-polar bundles were transformed into assemblies of polar bundles with ATP hydrolysis by HMM. Importantly, myosin subfragment-1 caused neither contraction nor transformation. These results are interpreted as follows. In the presence of ATP, the two-headed HMM molecule was able to cross-bridge antiparallel actin filaments, as well as parallel ones.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Molecular Biology, School of Science, Nagoya University, Aichi.
authors.forename: K
authors.initials: K
authors.lastname: Takiguchi
mesh_terms: D000199:Actins / Q000502:physiology* / Q000648:ultrastructure; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009132:Muscles / Q000502:physiology; D015879:Myosin Subfragments / Q000502:physiology* / Q000648:ultrastructure; D011817:Rabbits
chemical_list: D000199:Actins; D015879:Myosin Subfragments; D000255:Adenosine Triphosphate