title: Characterization of seven DA/DAPI-positive bisatellited marker chromosomes by in situ hybridization.
abstract: Seven dicentric bisatellited marker chromosomes, ascertained at amniocentesis, chorionic villus sampling, and in blood from an abnormal liveborn were characterized cytogenetically. All seven markers demonstrated brilliant bands by the DA/DAPI technique corresponding to C-band positive regions. Although some dicentric DA/DAPI-positive bisatellited markers have been identified as inverted duplicated 15s, recent literature has suggested that DA/DAPI lacks specificity for chromosome 15. Our evaluation of DA/DAPI-positive bisatellited marker chromosomes by in situ hybridization shows that some originate from chromosome 15 whereas DA/DAPI negative bisatellited markers may not be derived from 15. The morphological variations noted in our studies are discussed with respect to nomenclature.
authors.affiliation: Department of Medical Genetics, Indiana School of Medicine, Indianapolis 46202.
authors.forename: R
authors.initials: R
authors.lastname: Plattner
authors.forename: N A
authors.initials: NA
authors.lastname: Heerema
authors.forename: S R
authors.initials: SR
authors.lastname: Patil
authors.forename: P N
authors.initials: PN
authors.lastname: Howard-Peebles
authors.forename: C G
authors.initials: CG
authors.lastname: Palmer
mesh_terms: D000649:Amniocentesis; D002675:Child, Preschool; D015193:Chorionic Villi Sampling; D002871:Chromosome Banding; D002884:Chromosomes, Human, Pair 15; D015342:DNA Probes; D004276:DNA, Satellite / Q000032:analysis*; D004214:Distamycins; D005260:Female; D005456:Fluorescent Dyes; D005819:Genetic Markers; D006801:Humans; D007211:Indoles; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D011247:Pregnancy
chemical_list: D015342:DNA Probes; D004276:DNA, Satellite; D004214:Distamycins; D005456:Fluorescent Dyes; D005819:Genetic Markers; D007211:Indoles; C007293:DAPI; C030000:stallimycin