Avian LHRH during embryonic development: measurement by competitive ELISA with a monoclonal antibody.
A mouse monoclonal antibody directed against chicken Gln8-luteinizing hormone-releasing hormone (cLHRH-I) was developed and characterized. This antibody was used for the development of a competitive microtiter plate enzyme-linked immunosorbent assay for avian LHRH. The assay was validated for use with tissue and was used at a working range between 5 pg and 10 ng per sample. Using this procedure, cLHRH-I and II were assayed in whole brain extracts of Japanese Quail embryos. Samples were taken at regular intervals between Day 6 of incubation through Day 1 posthatch. There were 10 samples taken at each age with 2 replicates of the entire sampling regime. Data from males and females were pooled. LHRH concentrations were low, then rose to higher levels (15 pg/mg tissue) between Days 10 through 13 and decreased thereafter. These changes are likely to be correlated with the activation of the hypothalamic-pituitary-gonadal axis. This is particularly apparent in later embryonic development.
Li
Q C
QC
Department of Poultry Science, College of Agriculture, University of Maryland, College Park 20742-2315.
Alston-Mills
B
B
Ottinger
M A
MA
D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D001921:Brain / Q000196:embryology* / Q000378:metabolism; D003370:Coturnix / Q000196:embryology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis / Q000378:metabolism*; D008297:Male; D013997:Time Factors
D000911:Antibodies, Monoclonal; D007987:Gonadotropin-Releasing Hormone