Deficient utilization of succinate in a sterol 14 alpha-demethylation mutant of Candida albicans.
Growth of a wild-type strain (KD14) started after a lag of approximately 15 h when glucose-grown cells of Candida albicans were incubated at 25 degrees C, with succinate as a sole energy/carbon source. No growth was observed, however, for a sterol 14 alpha-demethylation mutant (KD4900). During the lag period, the wild-type cells retained their respiratory activity, as measured with glucose as an added substrate, and developed the capacity to incorporate labelled succinate. In contrast, incubation with succinate for 15 h resulted in diminished respiratory activity and failed to induce the succinate uptake capacity of the mutant cells. These two differences from the wild-type seem to account for the inability of the mutant to grow in the presence of a non-fermentable substrate.
Shimokawa
O
O
Department of Microbiology, Faculty of Dentistry, Kyushu University, Fukuoka, Japan.
Nakayama
H
H
D002176:Candida albicans / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D009154:Mutation; D010101:Oxygen Consumption; D013386:Succinates / Q000378:metabolism*
D003470:Culture Media; D013386:Succinates