title: A murine monoclonal anti-idiotypic antibody detects a common idiotope on human, mouse and rabbit antibodies to allergen Lol p IV.
abstract: A syngeneic mouse monoclonal anti-idiotypic antibody (anti-Id), designated as B1/1, was generated against a monoclonal antibody (MoAb 91) specific for Ryegrass pollen allergen Lol p IV. This anti-Id recognized an idiotope (Id) that was also present on other monoclonal antibodies with the same specificity as MoAb 91. Observations that (i) the anti-Id inhibited the binding of MoAb 91 to Lol p IV and (ii) the Id-anti-Id interaction could be inhibited by Lol p IV indicated that the Id was located within or near the antigen combining site. These properties served to characterize B1/1 as an internal image anti-Id. Evidence that an immune response in different species to Lol p IV elicits the formation of antibodies which express a common Id was provided by the observations that (i) the Id-anti-Id interactions could be inhibited by mouse, human and rabbit antisera to Lol p IV and (ii) the binding of these antisera to Lol p IV could be inhibited by the anti-Id. Interestingly, the internal image anti-Id B1/1 also recognized an Id on a monoclonal antibody which was directed to an epitope of Lol p IV, different from that recognized by MoAb 91.
authors.affiliation: Department of Immunology, University of Manitoba, Winnipeg, Canada.
authors.forename: E M
authors.initials: EM
authors.lastname: Zhou
authors.forename: J M
authors.initials: JM
authors.lastname: Dzuba-Fischer
authors.forename: E S
authors.initials: ES
authors.lastname: Rector
authors.forename: A H
authors.initials: AH
authors.lastname: Sehon
authors.forename: F T
authors.initials: FT
authors.lastname: Kisil
mesh_terms: D000485:Allergens; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000918:Antibody Specificity; D052179:Antigens, Plant; D006801:Humans; D051379:Mice; D010940:Plant Proteins; D011058:Pollen / Q000276:immunology*; D011817:Rabbits; D013045:Species Specificity
chemical_list: D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; C059297:Lol p IV protein, Lolium perenne; D010940:Plant Proteins