[Measurement of fluidity of membrane lipids in lymphocytes from patients with pulmonary tuberculosis].
The degree of fluidity of cellular membranes was determined by fluorescence polarization analysis using fluorescent probe 1,6-Dipheny-1,3,5-hexatriene (DPH), when embedded in membrane lipid region of intact cells. Results have shown that lymphocytes isolated from patients have a more fluid lipid layer in their cellular membrane (eta = 2.69 +/- 0.77 poise chi +/- s) than lymphocytes isolated from normal health (eta = 3.11 +/- 0.95 poise chi +/- s)(P less than 0.05). Increase in degree of membrane microviscosity was closely correlated with the rise in cells number.
Wang
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Department of Tuberculosis, PLA Hospital.
D002462:Cell Membrane; D004161:Diphenylhexatriene; D005260:Female; D005454:Fluorescence Polarization; D006801:Humans; D008214:Lymphocytes / Q000502:physiology*; D008297:Male; D008560:Membrane Fluidity / Q000502:physiology*; D008563:Membrane Lipids / Q000502:physiology*; D014397:Tuberculosis, Pulmonary / Q000097:blood*
D008563:Membrane Lipids; D004161:Diphenylhexatriene