title: Apoptosis of murine BW 5147 thymoma cells induced by dexamethasone and gamma-irradiation.
abstract: The mode and the kinetics of the death of T-thymoma cells upon dexamethasone treatment and gamma-irradiation (10Gy) have been studied using flow cytometry and biochemical analysis. It has been shown that the hormone and gamma-irradiation induce cell death by apoptosis. In both cases the cells are initially blocked in G2/M and die only after overcoming the blockage and cytokinesis. A short exposure to dexamethasone results in a cytostatic effect, whereas a cytotoxic effect is absent. Reducing serum concentration to 2% causes more rapid death both following gamma-irradiation and dexamethasone. These results are discussed in relation to cell death and proliferation.
authors.affiliation: Institute of Biological Physics, USSR Academy of Sciences, Moscow Region.
authors.forename: I I
authors.initials: II
authors.lastname: Kruman
authors.forename: N P
authors.initials: NP
authors.lastname: Matylevich
authors.forename: I P
authors.initials: IP
authors.lastname: Beletsky
authors.forename: V N
authors.initials: VN
authors.lastname: Afanasyev
authors.forename: S R
authors.initials: SR
authors.lastname: Umansky
mesh_terms: D000818:Animals; D001769:Blood; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D003470:Culture Media; D004273:DNA, Neoplasm / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D016195:G2 Phase / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays; D008938:Mitosis / Q000187:drug effects / Q000528:radiation effects; D013945:Thymoma / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D003470:Culture Media; D004273:DNA, Neoplasm; D003907:Dexamethasone