[The activation of the early chemiluminescent response of human neutrophils by joint treatment with tumor necrosis factor (TNF-alpha) and the calcium ionophore A-23187].
The influence of recombinant human tumor necrosis factor-alpha (TNF-alpha) and calcium ionophore A23187 on luminol- and lucigenin-dependent chemiluminescence capacity (CL) of human polymorphonuclear leukocytes (PMN) has been studied. The CL response of TNF-alpha treated PMN is amplified by lucigenin, but not luminol. TNF-alpha and A23287 synergistically induced both the luminol- and lucigenin-dependent early CL response. The combination of A23187 and activator of protein kinase C--phorbol (myristoyl-13-acetyl)--also provoked early CL response. While the combination of TNF-alpha and A23187 decreased late CL response compared to A23187 alone. The obtained results suggests that synergistic CL response of PMN induced by TNF-alpha and A23187 is connected with activation of protein kinase by TNF-alpha.
Shepetkin
I E
IE
Borunov
E V
EV
Skutina
I L
IL
Naumov
S A
SA
D000166:Acridines / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology*; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010100:Oxygen / Q000097:blood*; D011230:Precancerous Conditions / Q000097:blood; D011994:Recombinant Proteins / Q000494:pharmacology; D013274:Stomach Neoplasms / Q000097:blood; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D000166:Acridines; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C033472:10,10'-dimethyl-9,9'-biacridinium; D000001:Calcimycin; D008165:Luminol; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen
Aktivatsiia rannego khemiliuminestsentnogo otveta neĭtrofilov cheloveka sovmestnoĭ obrabotkoĭ faktorom nekroza opukholi (FNO-al'fa) i kal'tsievym ionoforom A23187.