[Clostridium difficile: a typing method for epidemiological studies].
One hundred and twenty two strains of Clostridium difficile were studied: 33 strains were isolated from stools of neonates or children, 89 cases of antibiotic associated diarrhea. The strains were identified by biochemical tests and gaz liquid chromatography of the fermentation products. A typing scheme for Clostridium difficile were proposed by using a polyacrylamide gel electrophoresis of whole cell proteins. We studied the correlation between this typing and the serogrouping scheme based on slide agglutination with a rabbit antisera and previously described by Delmée. The serogrouping defines 10 groups, PAGE allows the differentiation of 12 profiles, with a good correlation between the two methods for some serogroups except for the heterogenous serogroup A.
Miscopein
G
G
Laboratoire de Bactériologie, Centre Hospitalier Annecy.
Delmée
M
M
Sedallian
A
A
D001426:Bacterial Proteins / Q000737:chemistry*; D015373:Bacterial Typing Techniques; D002849:Chromatography, Gas; D016360:Clostridioides difficile / Q000145:classification* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D066298:In Vitro Techniques
D001426:Bacterial Proteins
Clostridium difficile: mise au point d'une méthode de typage pour les études épidémiologiques.