Isolation of intact mitochondria and hepatocytes using vibration.
A method of isolation of rat liver cells and mitochondria suggesting tissue disaggregation by vibration has been proposed. Mitochondria obtained using vibration have better parameters of oxidative phosphorylation as compared to homogenization. Hepatocytes isolated by vibration had viability about 90%, as determined by trypan blue exclusion, and rates of respiration and xenobiotic metabolism comparable to that observed by the enzymatic method.
Petrenko AYu
Institute for Problems of Cryobiology and Cryomedicine, USSR Academy of Sciences, Kharkov.
Sukach
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D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D008099:Liver / Q000166:cytology* / Q000648:ultrastructure; D008930:Mitochondria, Liver; D051381:Rats; D011919:Rats, Inbred Strains; D014732:Vibration