Effect of human T lymphotropic retrovirus-I exposure on cultured human glioma cell lines.
Four different human tumor cell lines of glial origin have been exposed to a human T lymphotropic retrovirus (HTLV-I). All these cell lines were positive for the glial marker glial fibrillary acidic protein (GFAP). The presence of virus RNA was demonstrated by in situ hybridization using an HTLV-I, SStI-SStI viral insert as probe. Virus expression has been monitored through an indirect immunofluorescence assay using a monoclonal antibody against virus core protein p19. All the four glioma cell lines tested became positive for p19 after 2 weeks of co-cultivation and showed a clear alteration of GFAP expression.
Macchi
B
B
Department of Experimental Medicine and Biochemical Science, University of Rome Tor Vergata, Italy.
Caronti
B
B
Pezzella
M
M
Bonmassar
E
E
Lauro
G
G
D000911:Antibodies, Monoclonal; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis / Q000276:immunology; D005910:Glioma / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D015368:Human T-lymphotropic virus 1; D006801:Humans; D009693:Nucleic Acid Hybridization; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology
D000911:Antibodies, Monoclonal; D005904:Glial Fibrillary Acidic Protein