Induction of demyelination by intraneural injection of antibodies against sulfoglucuronyl paragloboside.
Sulfoglucuronyl glycolipids (SGGLs) carry the glucuronyl 3-sulfate (HNK-1) epitope which is recognized by monoclonal IgM paraproteins from patients with demyelinating polyneuropathy. We report that intraneural injections of rat anti-SGGL antibodies induce demyelination in rat sciatic nerve, along with mild to moderate clinical symptoms. Morphologically, vesiculation and loosening of the myelin sheath were observed 3 h postinjection, followed by extensive demyelination and macrophage infiltration after 4 days. Since the anti-SGGL antibodies showed no cross-reactivity with other components in rat sciatic nerve, these results indicate that SGGLs alone can serve as the target antigens in demyelinating neuropathy.
Maeda
Y
Y
Department of Biochemistry and Molecular Biophysics, Medical College of Virginia, Commonwealth University, Richmond 23298.
Bigbee
J W
JW
Maeda
R
R
Miyatani
N
N
Kalb
R G
RG
Yu
R K
RK
D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000502:physiology; D005915:Globosides / Q000276:immunology*; D007267:Injections; D008297:Male; D008854:Microscopy, Electron; D009186:Myelin Sheath / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats; D011917:Rats, Inbred Lew; D012584:Sciatic Nerve / Q000648:ultrastructure; D013997:Time Factors
D000906:Antibodies; D005915:Globosides; C051917:sulfate-3-glucuronyl paragloboside