Molecular cloning of the ecotin gene in Escherichia coli.
The nucleotide sequence of a 876 bp region in E. coli chromosome that encodes Ecotin was determined. The proposed coding sequence for Ecotin is 486 nucleotides long, which would encode a protein consisting of 162 amino acids with a calculated molecular weight of 18,192 Da. The deduced primary sequence of Ecotin includes a 20-residue signal sequence, cleavage of which would give rise to a mature protein with a molecular weight of 16,099 Da. Ecotin does not contain any consensus reactive site sequences of known serine protease inhibitor families, suggesting that Ecotin is a novel inhibitor.
Lee
H R
HR
Department of Molecular Biology, College of National Sciences, Seoul National University, Korea.
Seo
J H
JH
Kim
O M
OM
Lee
C S
CS
Suh
S W
SW
Hong
Y M
YM
Tanaka
K
K
Ichihara
A
A
Ha
D B
DB
Chung
C H
CH
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D033903:Periplasmic Proteins; D021382:Protein Sorting Signals / Q000737:chemistry; D015183:Restriction Mapping
D001426:Bacterial Proteins; C039076:Eco protein, E coli; D029968:Escherichia coli Proteins; D033903:Periplasmic Proteins; D021382:Protein Sorting Signals