Sequence of gene choB encoding cholesterol oxidase of Brevibacterium sterolicum: comparison with choA of streptomyces sp. SA-COO.
The nucleotide (nt) sequence of the cholesterol oxidase (Cho)-encoding gene (choB) cloned from Brevibacterium sterolicum ATCC21387 was determined. The sequence contained an open reading frame with a G + C content of 64.9 mol% that would encode a protein of 552 amino acids (aa). Comparison of the nt sequence of choB and deduced aa sequence to those of the Cho-encoding gene (choA) of Streptomyces sp. strain SA-COO showed identities of 64% and 58%, respectively. N-terminal aa sequence analysis of the extracellular enzyme of B. sterolicum confirmed that the mature enzyme consisted of 507 aa with a predicted Mr of 54,902, and was preceded by a 45-aa signal sequence.
Ohta
T
T
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Japan.
Fujishiro
K
K
Yamaguchi
K
K
Tamura
Y
Y
Aisaka
K
K
Uwajima
T
T
Hasegawa
M
M
D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D001951:Brevibacterium / Q000201:enzymology* / Q000235:genetics; D002789:Cholesterol Oxidase / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
D021382:Protein Sorting Signals; D002789:Cholesterol Oxidase