Effect of ethanol on 15-hydroxyprostaglandin dehydrogenase activity in the brain stem of the near-term fetal sheep.
The effect of in vitro incubation with ethanol (10-80 mM) on 15-hydroxyprostaglandin dehydrogenase (15-OH-PGDH) activity was determined in the brain stem (pons and medulla) of the fetal sheep at a mean gestational age of 126 days (term: about 147 days). Enzyme activity was determined by measuring the rate of oxidation of PGE2 to 15-keto-PGE2 using a radiometric assay. There was no statistically significant ethanol-induced inhibition of 15-OH-PGDH activity. The data apparently do not support the hypothesis that acute in vitro exposure to ethanol directly inhibits 15-OH-PGDH activity in the near-term fetal brain stem.
Treissman
D
D
Department of Pharmacology and Toxicology, Faculty of Medicine, Queen's University, Kingston, Ont., Canada.
Smith
G N
GN
Patrick
J
J
Brien
J F
JF
D000818:Animals; D001933:Brain Stem / Q000187:drug effects* / Q000201:enzymology; D015232:Dinoprostone / Q000096:biosynthesis; D000431:Ethanol / Q000494:pharmacology*; D005333:Fetus / Q000187:drug effects* / Q000201:enzymology; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism*; D012756:Sheep
D000431:Ethanol; D006911:Hydroxyprostaglandin Dehydrogenases; D015232:Dinoprostone