Serum kinetics of crotoxin from Crotalus durissus terrificus venom in mice: evidence for a rapid clearance.
We report on an ELISA for the detection of crotoxin with a detection limit of 1-3 pg/ml of sample. Cross-reactivity with other animal venoms occurred only at concentrations above 1 microgram/ml. Serum kinetics of crotoxin were investigated in BALB/c mice after a single 10 micrograms s.c. dose of venom obtained from Crotalus durissus terrificus. Crotoxin levels were 254 +/- 141 ng/ml serum (X +/- S.E.) 15 min after venom injection, 3.9 +/- 0.5 ng/ml serum at 30 min and undetectable thereafter. The rapid clearance of crotoxin from the serum suggests that the test may be unsuitable for the clinical management of envenomation victims.
Barral-Netto
M
M
Servico de Imunologia, Hospital Universitario Prof. Edgard Santos, Universidade Federal da Bahia, Salvador-Bahia-Brazil.
von Sohsten
R L
RL
D000818:Animals; D003439:Crotoxin / Q000097:blood / Q000493:pharmacokinetics*; D004797:Enzyme-Linked Immunosorbent Assay; D008657:Metabolic Clearance Rate / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity; D013997:Time Factors
D003439:Crotoxin