Chemical induction of somatic gene mutations and chromosomal aberrations in lung fibroblasts of rats.
Rats can be used to detect both somatic gene mutations and chromosomal aberrations induced in vivo. The technique adapted from Chinese hamsters for the isolation and analysis of lung fibroblasts confirm the surprising results obtained in hamsters; namely, methyl methanesulphonate induces many micronuclei but no significant increase in gene mutations. In contrast ethylnitrosourea induces both micronuclei and gene mutations.
Khan
M A
MA
Department of Biology, York University, Toronto, Ont, Canada.
Heddle
J A
JA
D000818:Animals; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D005038:Ethylnitrosourea / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects*; D008168:Lung / Q000166:cytology; D008297:Male; D008741:Methyl Methanesulfonate / Q000633:toxicity*; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009154:Mutation; D051381:Rats; D011916:Rats, Inbred F344
D008741:Methyl Methanesulfonate; D005038:Ethylnitrosourea