Localization of L-alanine: 4,5-dioxovalerate transaminase in the matrix of the rat kidney mitochondria.
The bulk of the enzyme L-alanine: 4,5-dioxovalerate transaminase, which catalyses the transamination reaction between L-alanine and 4,5-dioxovalerate to synthesize delta-aminolevulinic acid was predominantly recovered in the mitochondrial matrix. Sub-fractionation procedure of the mitochondria involved the use of digitonin and lubrol followed by differential centrifugation to separate soluble and particulate enzymes. Lubrol did not inhibit this enzyme. Presence of this enzyme in the mitochondrial matrix was further confirmed by western blot analysis. The results support the conclusion that L-alanine: 4,5-dioxovalerate transaminase is localized and functions in the mitochondrial matrix.
Tyagi
R K
RK
Biochemistry Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.
Datta
K
K
D000818:Animals; D015153:Blotting, Western; D005591:Chemical Fractionation; D004072:Digitonin; D007668:Kidney / Q000201:enzymology*; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D011092:Polyethylene Glycols; D051381:Rats; D011919:Rats, Inbred Strains; D000637:Transaminases / Q000032:analysis* / Q000378:metabolism
D011092:Polyethylene Glycols; C005092:lubrol; D000637:Transaminases; C019687:aminolevulinate aminotransferase; D004072:Digitonin