[Study of heat denaturation of human serum albumin in water-alcohol and water-salt solutions in the presence of organic ligands].
The method of differential scanning microcalorimetry was used to show a decrease in heat stability of serum albumin in the presence of aliphatic alcohols. In aqueous-alcohol media, the melting temperature, denaturation transition enthalpy were decreased, and the protein intermolecular aggregation enhanced. When the alcohol concentration in aqueous solution was elevated, the number of epsilon-amino groups of lysine residues in human serum albumin exposed to the solvent rose from 6-7 in aqueous solution to maximum 20 groups in the aqueous-alcohol solution, respectively. The elevation of ionic strength also induced an increase in the number of exposed lysine residues and was accompanied by an enhancement of protein aggregation. The modification of six amino groups by pyridoxal phosphate or three by glucose in the initial albumin stabilized the protein incubated at 65 degrees-70 degrees C both in the aqueous-alcohol media. At the given concentration and temperature the native protein was denatured and fully aggregated. Aliphatic alcohols displaced fatty acids from the binding sites on the molecule of serum albumin, which resulted in a change in the number of peaks of the melting curve.
Stepuro
I I
II
Lapshina
E A
EA
Chaĭkovskaia
N A
NA
D000438:Alcohols / Q000737:chemistry; D001665:Binding Sites; D002152:Calorimetry, Differential Scanning; D002850:Chromatography, Gel; D005227:Fatty Acids / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D008024:Ligands; D011489:Protein Denaturation; D011732:Pyridoxal Phosphate / Q000737:chemistry; D012492:Salts / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry*; D012996:Solutions; D013816:Thermodynamics; D014867:Water / Q000737:chemistry
D000438:Alcohols; D005227:Fatty Acids; D008024:Ligands; D012492:Salts; D012709:Serum Albumin; D012996:Solutions; D014867:Water; D011732:Pyridoxal Phosphate; D005947:Glucose
Issledovanie teplovoĭ denaturatsii syvorotochnogo al'bumina cheloveka v vodno-spirtovykh i vodno-solovykh rastvorakh v prisutstvii organicheskikh ligandov.