title: Sperm count and motility influence the results of human fertilization in vitro.
abstract: In order to select sperm characteristics that can predict the outcome of in-vitro fertilization-embryo transfer (IVF-ET), semen samples delivered in conjunction with this treatment were studied carefully. We have analysed these data retrospectively in relation to the outcome of treatment. Ninety-one couples were treated for tubal infertility by IVF-ET. Fifteen women became pregnant. Sperm were isolated from semen using a swim-up technique and in most cases 40-80 x 10(3) (range 20-120 x 10(3)) motile sperm per ovum were used for insemination. The couples were divided into three groups: group A who achieved pregnancies, group B who achieved cleaved ova but no pregnancies, and group C who achieved no ova that were cleaved 48 h after oocyte recovery. Comparisons between these groups showed that some characteristics of the native semen samples and the swim-up preparations were significantly different: the sperm concentration (P = 0.001) and total sperm count (P = 0.003) in the native sample, the number of sperm recovered during 30 min of swim-up (P = 0.001), and the specific progressive motility of sperm in the swim-up preparation, both at the time of insemination and on each day, up to 5 days thereafter (P = 0.002-0.028). No pregnancy was achieved with a sperm concentration below 26 x 10(6) ml-1 in the native sample. Some of the sperm characteristics studied in this paper may be of value in the pretreatment evaluation for IVF treatment.
authors.affiliation: Department of Clinical Chemistry, Karolinska Institutet, Huddinge University Hospital, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Akerlöf
authors.forename: B
authors.initials: B
authors.lastname: Fredricsson
authors.forename: O
authors.initials: O
authors.lastname: Gustafson
authors.forename: N O
authors.initials: NO
authors.lastname: Lunell
authors.forename: L
authors.initials: L
authors.lastname: Nylund
authors.forename: L
authors.initials: L
authors.lastname: Rosenborg
authors.forename: H
authors.initials: H
authors.lastname: Slotte
authors.forename: A
authors.initials: A
authors.lastname: Pousette
mesh_terms: D000328:Adult; D002469:Cell Separation; D002970:Cleavage Stage, Ovum; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D011247:Pregnancy; D012189:Retrospective Studies; D013076:Sperm Count; D013081:Sperm Motility