Blood content in guinea-pig tissues: correction for the study of drug tissue distribution.
The blood content in different organs or tissues of guinea-pigs was determined by means of [125I]bovine serum albumin. The blood distribution expressed as percentage of total body blood for various organs or tissues ranged from 0.35 (adipose) to 17.5 (muscle)%. The blood content in tissue preparations expressed as the blood background correction factor, F (ml blood/g wet tissue) depended on the extent of bleeding during experiment; that factor can cause a considerable difference in the results of a tissue distribution study of a drug. Blood content was high in the lung (0.36 ml/g), heart, liver, kidney and spleen, but low in adipose and brain tissues (0.021 ml/g). The distribution of valproic acid in various tissues of guinea-pigs after intravenous injection was determined from the homogenates of isolated organs. The results showed that blood contamination can greatly alter the data of tissue distribution of a drug to as much as 25%. This study proposed a maximum (non-bled) and a minimum (extreme-bled) blood background correction factor for respective tissues of guinea-pigs, as well as equations for correcting the blood contamination in tissue during the drug distribution study. The results suggest that to obtain an exact analysis of drug concentrations in specific tissues, a correction for the blood of that specific tissue is necessary.
Yu
H Y
HY
School of Pharmacy, National Taiwan University, Taipei, R.O.C.
Chiu
Y W
YW
Lin
H H
HH
Chang
T C
TC
Shen
Y Z
YZ
D000818:Animals; D005260:Female; D006168:Guinea Pigs; D008297:Male; D012711:Serum Albumin, Radio-Iodinated; D014018:Tissue Distribution / Q000502:physiology*; D014635:Valproic Acid / Q000493:pharmacokinetics; D014781:Viscera / Q000098:blood supply*
D012711:Serum Albumin, Radio-Iodinated; D014635:Valproic Acid