title: Characterization of the degradation products of luteinizing hormone releasing hormone.
abstract: The degradation products of luteinizing hormone releasing hormone [LH/RH; 1; gonadorelin releasing hormone (GnRH); less than Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2] were determined in aqueous solution (pH 6.5) at 25, 37, 50, and 80 degrees C. The predominant route of degradation involved the cleavage of the less than Glu-His and Trp-Ser peptide bonds to give peptides 5-9 and hydrolysis of the terminal Gly-NH2 to the free acid form in peptides 4 and 10. Racemization of the serine and histidine residues to give peptides 2 and 3 was a second route of degradation.
authors.affiliation: R. W. Johnson Pharmaceutical Research Institute, Raritan, NJ 08869-0602.
authors.forename: M G
authors.initials: MG
authors.lastname: Motto
authors.forename: P F
authors.initials: PF
authors.lastname: Hamburg
authors.forename: D A
authors.initials: DA
authors.lastname: Graden
authors.forename: C J
authors.initials: CJ
authors.lastname: Shaw
authors.forename: M L
authors.initials: ML
authors.lastname: Cotter
mesh_terms: D000595:Amino Acid Sequence; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013696:Temperature
chemical_list: D010446:Peptide Fragments; D007987:Gonadotropin-Releasing Hormone