Striking homology of the 'variable' N-terminal as well as the 'conserved core' domains of the mouse and human TATA-factors (TFIID).
A complementary DNA (cDNA) encoding a mouse TFIID (mIID) was isolated from mouse brain cDNA libraries. The 316 amino acid sequence deduced from cDNA sequences revealed the presence of an amino-terminal region enriched in serine, threonine, and proline (STP-cluster), an uninterrupted stretch of 13 glutamine residues (Q-run), a second STP-cluster, and a conserved carboxy-terminal region. Amino acid sequences of the first STP-cluster and the conserved carboxy-terminal region were identical to those of the human TFIID (hIID). However, the Q-run was considerably shorter than that in hIID and sequences in the second STP-cluster diverged from those of the hIID. The murine TFIID transcript is expressed as a 2 kilobase poly(A)+ RNA in the mouse brain. Southern blot analysis identified a single gene copy per haploid mouse genome.
Tamura
T
T
Division of Behavior and Neurobiology, National Institute for Basic Biology, Okazaki, Japan.
Sumita
K
K
Fujino
I
I
Aoyama
A
A
Horikoshi
M
M
Hoffmann
A
A
Roeder
R G
RG
Muramatsu
M
M
Mikoshiba
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015139:Blotting, Southern; D004247:DNA; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000235:genetics*
D035362:Transcription Factor TFIID; D014157:Transcription Factors; D004247:DNA