Evaluation of human sperm-zona pellucida tight binding by presence of monoclonal antibodies to sperm antigens.
Characterized WHO monoclonal antibodies (MAbs) to human sperm antigens were evaluated as to whether they inhibited sperm-zona pellucida tight binding as assessed by the hemizona assay (HZA). Of the 26 MAbs tested, only one inhibited zona binding. The whole sperm-specific MAb inhibited zona binding by 70%. The MAb also caused strong agglutination. Two procedures, Sephadex column chromatography and papain digestion, were used to determine whether agglutination or steric hindrance was a factor in the capability of MAb to inhibit zona binding. However, inhibition remained comparable to previous results. The MAb did not prevent capacitation, nor calcium influx and the resulting increase in hyperactivated motility and acrosome reaction. Since its inhibitory influence is not due to agglutination factors, steric hindrance or prevention of normal pre-fertilization maturation, the MAb may be blocking a portion of the zona binding receptor and may be useful in elucidating sperm antigens important to sperm-egg interaction. The approach used in this study allows definition of sperm surface antigens involved in zona pellucida binding.
Mahony
M C
MC
Jones Institute for Reproductive Medicine, Eastern Virginia Medical School, Norfolk 23510.
Fulgham
D L
DL
Blackmore
P F
PF
Alexander
N J
NJ
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D000941:Antigens / Q000276:immunology; D002118:Calcium / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D013075:Sperm Capacitation; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000276:immunology / Q000378:metabolism*; D015044:Zona Pellucida / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000941:Antigens; D002118:Calcium