Cerebellar granule cells in culture exhibit a ganglioside-sialidase presumably linked to the plasma membrane.
Cerebellar granule cells differentiated in culture were incubated with ganglioside [3H-Sph]GD1a in order to have it inserted into the plasma membrane, internalized by endocytosis, and metabolized. The metabolites formed included GM1, product of GD1a desialosylation. No GM1 or other metabolites were present in the incubation medium, whereas with the lysosomal apparatus blocked by chloroquine, or GD1a endocytosis prevented at 4 degrees C, the only metabolite formed was GM1. These results suggest that GD1a desialosylation did not occur either extracellularly or intracellularly but likely, at the membrane level. Similar results were obtained with [3H-Gal]GD1b, whereas no degradation of [3H-NeuAc]GM1 took place in the presence of chloroquine or at 4 degrees C. In conclusion, cerebellar granule cells express in vivo a sialidase, presumably located on the cell surface, that affects GD1a and GD1b but not GM1.
Riboni
L
L
Department of Medical Chemistry and Biochemistry, Medical School, University of Milan, Italy.
Prinetti
A
A
Bassi
R
R
Tettamanti
G
G
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology*; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000201:enzymology*; D002738:Chloroquine / Q000494:pharmacology; D005677:G(M1) Ganglioside / Q000378:metabolism; D005732:Gangliosides / Q000378:metabolism; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000378:metabolism*; D051381:Rats; D012794:Sialic Acids / Q000378:metabolism
D005732:Gangliosides; D012794:Sialic Acids; C021298:ganglioside, GD1a; C033851:ganglioside, GD1b; D005677:G(M1) Ganglioside; D002738:Chloroquine; C036052:GM3 ganglioside sialidase; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid