title: Feeding diets containing 2% cheno- or urso-deoxycholic acid or cholestyramine to rats for two weeks alters intestinal morphology and bile acid absorption.
abstract: This study was undertaken to examine the effect of supplementing chow for 2 weeks with 2% cheno- (CDC) or ursodeoxycholic (UDC) acid or cholestyramine (CHOL) on the intestinal morphology and in vitro uptake of bile acids in adult rats. Food intake was higher in UDC and CHOL as compared with animals fed chow or CDC, or in animals pair-fed a chow-restricted diet (CRD). Body weight gain was lower in CDC and CRD but was unchanged by feeding UDC or CHOL. Jejunal mucosal surface area was similar in the five groups, although the ileal mucosal surface area was lower in UDC than in the other animals. Feeding UDC reduced the ileal uptake of cholic acid (C), taurocholic acid (TC), and glycocholic acid (GC). Feeding CDC had no effect on bile acid uptake except when compared with animals fed a chow-restricted diet. Feeding CHOL reduced the active ileal uptake of C, had no effect on the uptake of TC or GC or CDC, and was associated with increased uptake of stearic, linoleic, and linolenic acids. These effects were likely related to a direct effect of changes in the luminal bile acids rather than due to an indirect effect of the reduced food intake, since the ileal uptake of CDC and GC was greater in animals fed CDC than in those fed a chow-restricted diet with comparable weight gain. Thus, 2 weeks of feeding bile acids or bile acid binding agents may alter the form and function of the rat intestine, and as well may lead to changes in food intake and body weight gain.
authors.affiliation: Department of Medicine, University of Alberta, Edmonton, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Keelan
authors.forename: A B
authors.initials: AB
authors.lastname: Thomson
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D001835:Body Weight / Q000187:drug effects; D002635:Chenodeoxycholic Acid / Q000494:pharmacology*; D002792:Cholestyramine Resin / Q000494:pharmacology*; D002793:Cholic Acids / Q000494:pharmacology; D004032:Diet; D004435:Eating / Q000187:drug effects; D005260:Female; D006000:Glycocholic Acid / Q000378:metabolism; D007408:Intestinal Absorption / Q000187:drug effects; D007422:Intestines / Q000187:drug effects*; D007700:Kinetics; D051381:Rats; D011919:Rats, Inbred Strains; D014580:Ursodeoxycholic Acid / Q000494:pharmacology*
chemical_list: D001647:Bile Acids and Salts; D002793:Cholic Acids; D002635:Chenodeoxycholic Acid; D002792:Cholestyramine Resin; D014580:Ursodeoxycholic Acid; D006000:Glycocholic Acid