title: High-performance liquid chromatographic assay for the determination of 5-aminosalicylic acid and acetyl-5-aminosalicylic acid concentrations in endoscopic intestinal biopsy in humans.
abstract: A high-performance liquid chromatographic method for the simultaneous determination of 5-amino-salicylic acid (5-ASA) and N-acetyl-5-ASA (Ac-5-ASA) concentrations in endoscopic mucosal biopsy homogenates is presented. The mean recoveries of 5-ASA and Ac-5-ASA from spiked blank biopsy homogenates ranged from 95.9 to 120% and from 92.5 to 100%, respectively. The coefficients of variation for 5-ASA and Ac-5-ASA were 0.7-8.6% and 1.4-12.9%, respectively. This method is useful for direct determination of topical availability of 5-ASA and Ac-5-ASA and probably an accurate parameter of drug bioavailability.
authors.affiliation: University Hospital Ghent, Department of Gastroenterology, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: De Vos
authors.forename: H
authors.initials: H
authors.lastname: Verdievel
authors.forename: R
authors.initials: R
authors.lastname: Schoonjans
authors.forename: R
authors.initials: R
authors.lastname: Beke
authors.forename: G A
authors.initials: GA
authors.lastname: De Weerdt
authors.forename: F
authors.initials: F
authors.lastname: Barbier
mesh_terms: D000636:Aminosalicylic Acids / Q000032:analysis*; D001706:Biopsy; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D016099:Endoscopy, Gastrointestinal; D006801:Humans; D007413:Intestinal Mucosa / Q000737:chemistry*; D019804:Mesalamine
chemical_list: D000636:Aminosalicylic Acids; D019804:Mesalamine; C028836:N-acetyl-5-aminosalicylic acid