A novel cationic liposome reagent for efficient transfection of mammalian cells.
A novel cationic derivative of cholesterol, 3 beta [N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol), has been synthesized and used to prepare sonicated liposomes with dioleoylphosphatidylethanolamine. This novel cationic liposome reagent facilitates efficient DNA mediated transfection in A431 human epidermoid carcinoma cells, A549 human lung carcinoma cells, L929 mouse fibroblast cells, and YPT minipig primary endothelial cells. The activity was greater than that of a commercial reagent, Lipofectin, and was approximately 4-fold less toxic than Lipofectin when assayed with A431 cells. The reagent is easy to synthesize and stable for at least 6 weeks.
Gao
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Department of Biochemistry, University of Tennessee, Knoxville 37996-0840.
Huang
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D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D004247:DNA / Q000032:analysis; D006801:Humans; D007202:Indicators and Reagents; D008081:Liposomes; D008322:Mammals; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D014162:Transfection
D007202:Indicators and Reagents; D008081:Liposomes; D010714:Phosphatidylethanolamines; C070648:3-(N-(N',N'-dimethylaminoethane)carbamoyl)cholesterol; C020888:1,2-dielaidoylphosphatidylethanolamine; D004247:DNA; D002784:Cholesterol; D015500:Chloramphenicol O-Acetyltransferase