Anthracycline antibiotic 2-hydroxyaclacinomycins. I. 2-Hydroxyaclacinomycin-producing recombinant obtained from aclacinomycin-blocked mutants of Streptomyces galilaeus by a technique of protoplast fusion.
The technique of protoplast fusion which optimized prototrophic recombination in aclacinomycin-producing Streptomyces galilaeus was studied and applied to the construction of new anthracycline analog-producing recombinant upon genetic cross of two specific mutants blocked in aclacinomycin biosynthesis. Thus, 2-hydroxyaclacinomycin-producing recombinant was obtained by the protoplast fusion.
Yoshimoto
A
A
Central Research Laboratories, Mercian Corporation, Kanagawa, Japan.
Johdo
O
O
Ishikura
T
T
Takeuchi
T
T
D015250:Aclarubicin / Q000031:analogs & derivatives*; D000903:Antibiotics, Antineoplastic; D009154:Mutation; D011995:Recombination, Genetic; D013302:Streptomyces / Q000235:genetics*
D000903:Antibiotics, Antineoplastic; D015250:Aclarubicin; C003424:2-hydroxyaclacinomycin A