O-hydroxyphenylacetaldehyde: a major novel metabolite of coumarin formed by rat, gerbil and human liver microsomes.
A major novel coumarin metabolite was isolated from rat hepatic microsomal incubations by high-performance liquid chromatography. In the presence of a rat liver cytosolic fraction and NADH it was rapidly metabolized to O-hydroxyphenylethanol. The metabolite co-chromatographed with an authentic sample of O-hydroxyphenylacetaldehyde and its identity was confirmed by mass spectral analysis. The formation of O-hydroxyphenylacetaldehyde from coumarin was NADPH-dependent. It was the major metabolite formed by rat, gerbil and human liver microsomes at a coumarin concentration of 1mM.
Fentem
J H
JH
Department of Physiology & Pharmacology, University of Nottingham, U.K.
Fry
J R
JR
Whiting
D A
DA
D000079:Acetaldehyde / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005849:Gerbillinae; D006801:Humans; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D008862:Microsomes, Liver / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D003374:Coumarins; D009243:NAD; C070647:2-hydroxyphenylacetaldehyde; C030123:coumarin; D000079:Acetaldehyde