Antibody to Pseudomonas pseudomallei exotoxin in sheep exposed to natural infection.
Specific antibody to Pseudomonas pseudomallei exotoxin was detected in sheep sera exposed to natural infection. An enzyme-linked immunosorbent assay (ELISA) was used. Serum antitoxin was present in 49.3% of sera obtained from a flock of sheep naturally exposed to P. pseudomallei infection. Among these sera, 17.0% gave titers of 10,000. In contrast, serum antitoxin was present in only 6.0% of sera collected from sheep kept on a melioidosis-free farm. The ELISA reactivity of all positive sera could be completely absorbed with purified P. pseudomallei exotoxin. Similarly, preincubation of the exotoxin-coated wells with specific antiserum inhibited the ELISA reactivity of sheep sera. The results indicate that exotoxin is produced in vivo during infection by P. pseudomallei.
Ismail
G
G
Faculty of Science and Natural Resources, Universiti Kebangsaan, Sabah, Malaysia.
Mohamed
R
R
Rohana
S
S
Sharifah
H S
HS
Embi
N
N
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000992:Antitoxins / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005098:Exotoxins / Q000276:immunology*; D008554:Melioidosis / Q000276:immunology / Q000662:veterinary*; D011549:Pseudomonas / Q000276:immunology*; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*
D000907:Antibodies, Bacterial; D000992:Antitoxins; D005098:Exotoxins