title: How osteoblasts become osteocytes: a decreasing matrix forming process.
abstract: Osteocyte matrix inclusion process was studied in an in vitro woven bone nodule formation model where a large number of osteocytes at different degrees of maturation were examined. This work focused on early stages of osteocyte inclusion. This matrix inclusion occurred without a matrix synthesis inversion by the future osteocyte and with maintenance of close cell contacts with the replacing cell. A passive matrix embedding process related to a decreased activity of the osteoblast-osteocyte cell is proposed as a comprehensive pathway from osteoblast to osteocyte. The formation of the osteocyte is therefore presented as a very coordinated space and time related cell-cell interaction between cells of the three cell pools of the bone.
authors.affiliation: Laboratoire de Biologie-Odontologie, Université Paris VII, Paris, France.
authors.forename: J R
authors.initials: JR
authors.lastname: Nefussi
authors.forename: J M
authors.initials: JM
authors.lastname: Sautier
authors.forename: V
authors.initials: V
authors.lastname: Nicolas
authors.forename: N
authors.initials: N
authors.lastname: Forest
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000166:cytology*; D002450:Cell Communication / Q000502:physiology; D002453:Cell Cycle; D002478:Cells, Cultured; D005333:Fetus; D010006:Osteoblasts / Q000502:physiology*; D010011:Osteocytes / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012886:Skull