Flow-dependent extraction of 1-naphthol by the rat isolated perfused kidney.
The influence of variation of perfusion flow rate on the renal clearance of p-aminohippuric acid and 1-naphthol was studied with an isolated perfused rat kidney preparation. Kidney functions were well maintained at low perfusion flow rates by the use of a fluorocarbon emulsion to increase the oxygen capacity of the perfusion buffer. Renal extraction of p-aminohippuric acid decreased with increasing perfusion flow. Our data show that at high perfusion flow rates maximal extractable perfusion flow forms only a small part of the total perfusion flow. 1-Naphthol is rapidly metabolized to its glucuronide and sulfate conjugate in the isolated perfused rat kidney. Using PAH as a marker for the maximal extractable perfusion flow, 1-naphthol could be regarded as a high-extraction compound even at high perfusion flow rates. Our results suggest that p-aminohippuric acid clearance, rather than total perfusion flow rate, should be used as the measure of maximal extractable blood flow for the estimation of extraction ratio in the isolated perfused kidney of compounds excreted or metabolized by the proximal tubules.
Redegeld
F A
FA
Department of Pharmacology, Faculty of Pharmacy, University of Utrecht, The Netherlands.
Hofman
G A
GA
Koster
A S
AS
Noordhoek
J
J
D000818:Animals; D007668:Kidney / Q000378:metabolism* / Q000502:physiology; D008297:Male; D009284:Naphthols / Q000378:metabolism / Q000493:pharmacokinetics*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012079:Renal Circulation / Q000502:physiology; D010130:p-Aminohippuric Acid / Q000493:pharmacokinetics
D009284:Naphthols; C029350:1-naphthol; D010130:p-Aminohippuric Acid