Identification of a specific manganese peroxidase among ligninolytic enzymes secreted by Phanerochaete chrysosporium during wood decay.
The specific enzymes associated with lignin degradation in solid lignocellulosic substrates have not been identified. Therefore, we examined extracts of cultures of Phanerochaete chrysosporium that were degrading a mechanical pulp of aspen wood. Western blot (immunoblot) analyses of the partially purified protein revealed lignin peroxidase, manganese-dependent peroxidase (MnP), and glyoxal oxidase. The dominant peroxidase, an isoenzyme of MnP (pI 4.9), was isolated, and its N-terminal amino acid sequence and amino acid composition were determined. The results reveal both similarities to and differences from the deduced amino acid sequences from cDNA clones of dominant MnP isoenzymes from liquid cultures. Our results suggest, therefore, that the ligninolytic-enzyme-encoding genes that are expressed during solid substrate degradation differ from those expressed in liquid culture or are allelic variants of their liquid culture counterparts. In addition to lignin peroxidase, MnP, and glyoxal oxidase, xylanase and protease activities were present in the extracts of the degrading pulp.
Datta
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University of Wisconsin Biotechnology Center, Madison.
Bettermann
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Kirk
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D000429:Alcohol Oxidoreductases / Q000302:isolation & purification / Q000648:ultrastructure; D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000201:enzymology* / Q000378:metabolism; D001673:Biodegradation, Environmental; D015153:Blotting, Western; D002852:Chromatography, Ion Exchange; D005656:Fungal Proteins / Q000302:isolation & purification* / Q000648:ultrastructure; D007700:Kinetics; D008031:Lignin / Q000378:metabolism*; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000302:isolation & purification* / Q000378:metabolism / Q000648:ultrastructure; D014934:Wood
D005656:Fungal Proteins; D008031:Lignin; D000429:Alcohol Oxidoreductases; C052371:glyoxal oxidase; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase