Toxoplasmacidal activity of macrophages activated by recombinant major surface antigen (P30) of Toxoplasma gondii.
Recombinant major surface antigen (P30), which was produced as a glutathione S-transferase (EC 2.5.1.18) fusion protein of Toxoplasma gondii, was found to be able to activate macrophages to kill T. gondii in vitro. The macrophage activation was due to P30 in the fusion protein, not to glutathione S-transferase.
Makioka
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Department of Parasitology, Jikei University School of Medicine, Tokyo, Japan.
Kobayashi
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D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005982:Glutathione Transferase / Q000235:genetics; D007114:Immunization; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D014122:Toxoplasma / Q000276:immunology*
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; C047168:SAG1 antigen, Toxoplasma; D005982:Glutathione Transferase