title: Micellar electrokinetic capillary chromatographic separation and laser-induced fluorescence detection of 2'-deoxynucleoside 5'-monophosphates of normal and modified bases.
abstract: Micellar electrokinetic chromatography is used to separate dansylated nucleotides, both normal and modified species. The high separation power allows detection of minor components present in less than 1 part per thousand of the major components. Laser-excited fluorescence is used to detect the separated components at the 6 x 10(-18) mol level or 10(-9) M injected material. Combined with high-performance liquid chromatographic enrichment prior to labeling, this technique can be used to assess DNA damage in carcinogenesis studies.
authors.affiliation: Department of Chemistry, USDOE, Iowa State University, Ames 50011.
authors.forename: T
authors.initials: T
authors.lastname: Lee
authors.forename: E S
authors.initials: ES
authors.lastname: Yeung
authors.forename: M
authors.initials: M
authors.lastname: Sharma
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003854:Deoxyribonucleotides / Q000032:analysis*; D004586:Electrophoresis / Q000379:methods*; D005453:Fluorescence; D007834:Lasers
chemical_list: D003854:Deoxyribonucleotides