Expression of chicken integrin beta 1 subunit in rat PC12 cells.
We transfected rat pheochromocytoma (PC12) cells with a cDNA encoding chicken integrin beta 1 subunit. The chicken integrin beta 1 subunit produced in stable transfectants associated with two major alpha subunits of rat integrins to form interspecific chimeric receptors. These receptors mediated cell spreading and initial neurite outgrowth on laminin as did corresponding endogenous integrins, although they were slightly less effective in inducing cell adhesion to laminin. These results indicate that chicken integrin beta 1 may functionally substitute for beta 1 subunit of rat integrins in PC12 cells. Apparently, the structure of the integrin beta 1 subunit is highly conserved in the evolution of these species.
Hayashi
Y
Y
Biochemical Research Institute, Morinaga Milk Ind. Co. LTD, Kanagawa, Japan.
Reszka
A
A
Iguchi
T
T
Reinish
B
B
Kawashima
T
T
Horwitz
A F
AF
D000310:Adrenal Gland Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002645:Chickens; D002678:Chimera / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D016023:Integrins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D007797:Laminin / Q000494:pharmacology; D009414:Nerve Growth Factors / Q000494:pharmacology; D010673:Pheochromocytoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D011233:Precipitin Tests; D014162:Transfection / Q000235:genetics; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure
D004273:DNA, Neoplasm; D016023:Integrins; D007797:Laminin; D009414:Nerve Growth Factors