Determination of vitamin E in animal feedingstuffs by high-performance liquid chromatography. Analytical Methods Committee.
A method is given for the determination of vitamin E in animal feeds and pet foods. The sample is saponified with ethanolic potassium hydroxide solution and the vitamin E extracted into light petroleum (boiling range 40-60 degrees C). This solution is evaporated to dryness under reduced pressure and the residue dissolved in hexane. The concentration of vitamin E in this solution is determined by high-performance liquid chromatography using a silica column and an isooctane-propan-2-ol mixture as the mobile phase. An alternative method using hexane-1,4-dioxane as the mobile phase is also given.
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000829:Animals, Domestic; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000431:Ethanol; D006878:Hydroxides; D011188:Potassium; D017680:Potassium Compounds; D013552:Swine; D014810:Vitamin E / Q000032:analysis*
D006878:Hydroxides; D017680:Potassium Compounds; D014810:Vitamin E; D000431:Ethanol; D011188:Potassium; C029943:potassium hydroxide