title: Clinical results and characterization of tumor-infiltrating lymphocytes with or without recombinant interleukin 2 in human metastatic renal cell carcinoma.
abstract: A Phase I trial of tumor-infiltrating lymphocytes (TIL) expanded in vitro and administered on Days 1 and 8, with or without continuous infusion recombinant interleukin 2 (rIL-2) in 25 patients with metastatic renal cell carcinoma, was conducted. Eighteen of the 25 eligible patients were treated with TIL and escalating doses of rIL-2 (0.0, 3.0, 4.5 x 10(6) units/m2) on Days 1 to 5 and 8 to 12. Dose-limiting toxicity was pulmonary, and the maximum tolerated dose of rIL-2 was 3.0 x 10(6) units/m2. No clinical responses were observed. Immunological monitoring of peripheral blood lymphocytes demonstrated significant increases in CD3+ and CD56+ cells, including the activated T-cell subsets. Phenotypic analysis of cultured TILs demonstrated significant heterogeneity and the presence of CD3+CD4+ and CD3+CD8+ T-cells, with CD3-CD56+ and CD3+CD56+ populations also present. The majority of cultured TILs expressed HLA-DR and CD45RO, with a variable number expressing CD25. The rIL-2-expanded TILs possessed cytotoxicity against allogeneic and autologous tumor, with cytolytic activity against only autologous tumor seen in one patient. Results demonstrate that in vitro expansion of TILs is possible, but further studies are needed to define the biology of TILs in renal cancer and to isolate and expand tumor-specific T-cells.
authors.affiliation: Department of Experimental Therapeutics, Cleveland Clinic, Ohio 44195-5236.
authors.forename: R M
authors.initials: RM
authors.lastname: Bukowski
authors.forename: W
authors.initials: W
authors.lastname: Sharfman
authors.forename: S
authors.initials: S
authors.lastname: Murthy
authors.forename: P
authors.initials: P
authors.lastname: Rayman
authors.forename: R
authors.initials: R
authors.lastname: Tubbs
authors.forename: J
authors.initials: J
authors.lastname: Alexander
authors.forename: G T
authors.initials: GT
authors.lastname: Budd
authors.forename: J S
authors.initials: JS
authors.lastname: Sergi
authors.forename: L
authors.initials: L
authors.lastname: Bauer
authors.forename: V
authors.initials: V
authors.lastname: Gibson
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D002292:Carcinoma, Renal Cell / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D002460:Cell Line; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D004341:Drug Evaluation; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000009:adverse effects*; D007376:Interleukin-2 / Q000627:therapeutic use / Q000633:toxicity*; D007680:Kidney Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011994:Recombinant Proteins / Q000627:therapeutic use / Q000633:toxicity; D016176:T-Lymphocyte Subsets / Q000276:immunology
chemical_list: D015703:Antigens, CD; D006684:HLA-DR Antigens; D007376:Interleukin-2; D011994:Recombinant Proteins