title: Improved procedure for the second-derivative spectrophotometric analysis of carboxyhaemoglobin.
abstract: We describe a modification of the second-derivative spectrophotometric assay for carboxyhaemoglobin in blood. Using the original procedure we have often observed a time-dependent change in the signal leading to unreliable results. By using phosphate-buffered-saline (pH 7.4) instead of ammonia to dissolve the deoxygenating agent (sodium dithionite), we obtained stable and reproducible second-derivative signals from which the percentage carboxyhaemoglobin in the patients' samples can be calculated.
authors.affiliation: Department of Clinical Chemistry, Streekziekenhuis Midden-Twente, Hengelo, The Netherlands.
authors.forename: H
authors.initials: H
authors.lastname: Verweij
authors.forename: H A
authors.initials: HA
authors.lastname: Bonte
mesh_terms: D001774:Blood Chemical Analysis; D002263:Carboxyhemoglobin / Q000032:analysis*; D005069:Evaluation Studies as Topic; D006801:Humans; D013053:Spectrophotometry
chemical_list: D002263:Carboxyhemoglobin