Localization of the gene encoding steroid hydroxylase cytochrome P-450 from Rhizopus nigricans inside a HindIII fragment of genomic DNA.
The gene encoding steroid inducible cytochrome P450 of Rhizopus nigricans ATCC 6227b has been found inside a HindIII fragment of the genomic DNA by hybridization with a partial length cDNA probe. The latter was isolated by immunoscreening a cDNA library prepared in the lambda gt11 expression system and identified on the basis of inducibility and sequence analysis. The nucleotide sequence of the cDNA probe revealed a coding sequence for the heme binding segment characteristic of the P450 gene family.
Breskvar
K
K
Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Yugoslavia.
Cresnar
B
B
Plaper
A
A
Hudnik-Plevnik
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D015247:Deoxyribonuclease HindIII; D015723:Gene Library; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012233:Rhizopus / Q000201:enzymology / Q000235:genetics*; D013250:Steroid Hydroxylases / Q000235:genetics*
D004271:DNA, Fungal; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D015247:Deoxyribonuclease HindIII