Isolation and characterization of zinc coproporphyrin I: a major fluorescent component in meconium.
We isolated a substance from an organic extract of meconium (in neutral pH) that exhibited a porphyrin-like fluorescence peak at 580 nm on excitation at 405 nm and deduced its structure. Spectrometric data suggest that the substance is not free coproporphyrin I or free coproporphyrin III, but is a chelate of coproporphyrin I with Zn2+. We also detected a chelate of coproporphyrin III with Zn2+ by HPLC. These substances may be useful as new indicators of the presence of meconium.
Horiuchi
K
K
Department of Chemistry, Hamamatsu University School of Medicine, Japan.
Adachi
K
K
Fujise
Y
Y
Naruse
H
H
Sumimoto
K
K
Kanayama
N
N
Terao
T
T
D000653:Amniotic Fluid / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003306:Coproporphyrins / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008470:Meconium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013054:Spectrophotometry, Atomic; D015032:Zinc / Q000737:chemistry / Q000302:isolation & purification*
D003306:Coproporphyrins; C026971:coproporphyrin III; C031467:coproporphyrin I; D015032:Zinc