Protein binding of indomethacin in human cerebrospinal fluid.
The binding of the non-steroidal anti-inflammatory drug indomethacin to proteins in human cerebrospinal fluid (CSF), drawn during lumbar puncture from 10 patients affected by lumbosciatica, was measured by equilibrium dialysis and spectrofluorimetry. Similar binding studies on human serum albumin solutions (0.5 and 1 g/L) were performed using the same techniques. The mean binding percentage of indomethacin determined by equilibrium dialysis was 40%. The results obtained by both techniques allowed us to conclude that the binding of indomethacin in CSF was essentially due to albumin.
Muller
N
N
URA CNRS 1288, Physiopathologie et Pharmacologie Articulaires, Faculté de Médicine, Vandoeuvre Les Nancy, France.
Lapicque
F
F
Monot
C
C
Payan
E
E
Gillet
P
P
Bannwarth
B
B
Netter
P
P
D003956:Dialysis; D006801:Humans; D007213:Indomethacin / Q000134:cerebrospinal fluid* / Q000378:metabolism; D007700:Kinetics; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000134:cerebrospinal fluid / Q000378:metabolism
D012709:Serum Albumin; D014364:Tryptophan; D007213:Indomethacin