Flow cytometric analysis of anti-platelet antibodies in patients with chronic idiopathic thrombocytopenic purpura (ITP) using acid-treated, formalin-fixed platelets.
Acid treatment of normal washed platelets prepared by albumin density gradient centrifugation followed by formalin fixation effectively eliminates HLA class I antigens from the platelet surface, without altering the immunoreactivity of platelet membrane glycoproteins Ib and IIb/IIIa complex. Using these platelets, flow cytometric analysis was performed on a total of 20 sera from different patients with chronic ITP, and revealed an inverse linear correlation between the platelet count and the mean fluorescence intensity which represents a relative amount of platelet-bound IgG. These results suggest that acid-treated, formalin-fixed platelets can be used as specimens to detect anti-platelet antibodies to differentiate from those to HLA-class I antigens.
Mizumoto
Y
Y
Department of the Second Internal Medicine, Nara Medical College, Osaka University Hospital, Japan.
Fujimura
Y
Y
Nishikawa
K
K
Uchida
M
M
Fukui
H
H
Morii
T
T
Narita
N
N
Kurata
Y
Y
D000143:Acids / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000906:Antibodies / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D001792:Blood Platelets / Q000276:immunology*; D002648:Child; D005260:Female; D005434:Flow Cytometry; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010980:Platelet Membrane Glycoproteins / Q000187:drug effects; D011696:Purpura, Thrombocytopenic / Q000276:immunology*
D000143:Acids; D000906:Antibodies; D000954:Antigens, Surface; D006680:HLA Antigens; D010980:Platelet Membrane Glycoproteins