Sandwich enzyme immunoassay for rat transferrin with two monoclonal antibodies and its application.
The development of a sandwich enzyme immunoassay for rat transferrin with two monoclonal antibodies is described. Microtiter plates coated with one monoclonal antibody (15C2H3) were used, and captured transferrin was estimated with a horseradish peroxidase-conjugated Fab' fragment of another monoclonal antibody (22A06D2). In this assay, the measurable range is 5-150 ng/ml and the coefficients of variation within and between the assay series are 1.2-5.0 and 3.3-6.0%, respectively. Recovery was 101 +/- 9.7% when purified rat transferrin was added to rat plasma. No cross-reactivity with bovine, human, or mouse transferrin was shown. This assay for rat transferrin is a highly specific, sensitive, and expeditious method which may allow routine analysis of rat transferrin in blood or culture supernatants of rat hepatocytes.
Matsumoto
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T
Department of Biochemistry, Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Company Ltd., Osaka, Japan.
Shima
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H
Kishi
T
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Sato
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D008099:Liver / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000032:analysis / Q000096:biosynthesis; D014168:Transferrin / Q000032:analysis* / Q000096:biosynthesis
D000911:Antibodies, Monoclonal; D012709:Serum Albumin; D014168:Transferrin