[Detection of cancer-associated antigen in feces of patients with gastric cancer by monoclonal antibodies].
Detection of cancer-associated antigen in feces was performed by ELISA binding inhibition method using monoclonal antibody in 25 patients with gastric cancer, 70 with non-malignant gastrointestinal diseases and 100 healthy individuals. Monoclonal antibodies used were CL-4, PS-9, PS-10 and a "cocktail" of the three. The level of cancer-associated antigens detected in feces was significantly higher in patients with gastric cancer than in healthy individuals. PS-9 and PS-10 were also significantly higher in gastric cancer than in non-malignant gastrointestinal diseases. The positive rates of CL-4 were 62.5% vs 7.0%, PS-9 44.0% vs 3% and PS-10 64.0% vs 7.0% in patients with gastric cancer and healthy individuals. When the cocktail of the three was used, the positive rates increased to 88.0% and 14% in the above two groups and 26.6% in gastritis group. These results indicate that the detection of cancer-associated antigen in feces is of value in diagnosis of gastric cancer.
Yuan
M
M
General Hospital, PLA, Beijing.
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D005243:Feces / Q000737:chemistry*; D006801:Humans; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor