title: The role of the adenylyl cyclase system in the regulation of corpus luteum function in the human and in nonhuman primates.
abstract: We have reviewed the properties of luteinizing hormone/human chorionic gonadotropic (LH/hCG)-sensitive adenylyl cyclase (AC) of human corpus luteum (CL) and its regulation by several hormones and nonhormonal activators. We have also described the changes in enzyme activity in membrane preparations of human and cynomolgus monkey CL obtained at various stages of the menstrual cycle and pregnancy. The data have been analyzed with respect to the functional status of the luteal tissue and to the species differences among primate CL. In the menstrual cycle, luteal AC responsiveness to LH/hCG was detectable during the midluteal phase, but not during the late luteal phase or in the follicular phase of the following cycle. In addition, nonhormonal stimulation was high in CL obtained during the midluteal and late luteal phases, but declined drastically by the follicular phase of the next cycle. In early pregnancy, the enzyme was unresponsive to LH/hCG stimulation, yet its sensitivity to nonhormonal stimulation was similar, if not identical, to that of midluteal phase CL. Functional activity was also evident at the end of pregnancy. These results demonstrate that expression of AC activity in primate luteal membrane changes significantly with varying hormonal status under physiologic conditions. It is concluded that the AC system in luteal membranes is an effective model to study the mechanisms that regulate function and life span of the human and nonhuman primate CL.
authors.affiliation: University of California Irvine Medical Center, Department of Obstetrics and Gynecology, Orange 92668.
authors.forename: F J
authors.initials: FJ
authors.lastname: Rojas
authors.forename: I
authors.initials: I
authors.lastname: Moretti-Rojas
authors.forename: J P
authors.initials: JP
authors.lastname: Balmaceda
authors.forename: R H
authors.initials: RH
authors.lastname: Asch
mesh_terms: D000262:Adenylyl Cyclases / Q000502:physiology*; D000818:Animals; D006063:Chorionic Gonadotropin / Q000502:physiology; D003338:Corpus Luteum / Q000201:enzymology / Q000502:physiology*; D005260:Female; D006801:Humans; D008597:Menstrual Cycle / Q000378:metabolism; D011247:Pregnancy; D011323:Primates / Q000502:physiology*; D013045:Species Specificity
chemical_list: D006063:Chorionic Gonadotropin; D000262:Adenylyl Cyclases