Functional equivalence of cryptococcal and haptene-specific T suppressor factor (TsF). II. Monoclonal anti-cryptococcal TsF inhibits both phagocytosis by a subset of macrophages and transfer of contact sensitivity.
Monoclonal anti-cryptococcal TsF (which inhibits phagocytosis by macrophages) and anti-picryl TsF use the same two circuits to block the transfer of contact sensitivity (CS). Both arm macrophages which then release a macrophage suppressor factor (MSF) when exposed to antigen. This MSF depresses the transfer of CS. The evidence suggests that a single molecular species of TsF (MW ca. 70 kDa), which bears an antigen-binding site and I-J determinant, is responsible for MSF production and inhibition of phagocytosis. Anti-cryptococcal TsF also arms the T acceptor cell which then releases nsTsF-1 after triggering with a specific antigen (SCPA). This nsTsF-1, which depresses the transfer of contact sensitivity, was authentic, as shown by its I-J positivity (in contrast to MSF) and its role in the production of nsTsF-2. As anti-picryl TsF also inhibits phagocytosis, it was concluded that anti-cryptococcal TsF, originally detected by the inhibition of phagocytosis, and anti-picryl TsF, originally detected by inhibition of CS, are functionally equivalent.
Blackstock
R
R
Division of Immunological Medicine, Clinical Research Centre, Harrow, Great Britain.
Zembala
M
M
Asherson
G L
GL
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D003454:Cryptococcus / Q000276:immunology*; D003877:Dermatitis, Contact / Q000276:immunology*; D006241:Haptens; D006825:Hybridomas; D007116:Immunization, Passive; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D010587:Phagocytosis; D010851:Picrates / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000276:immunology; D013491:Suppressor Factors, Immunologic / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000942:Antigens, Bacterial; D006241:Haptens; D010851:Picrates; D011135:Polysaccharides, Bacterial; D013491:Suppressor Factors, Immunologic; C005858:picric acid