[Analysis of components of immune complexes in sera of patients with systemic lupus erythematosus].
The samples of immune complexes (IC) isolated through Clq tube from serum of SLE patients were analysed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting for their antigens and antibodies. The isolated IC contained gamma, mu and L chain of immunoglobulin and various sizes of DNA ranging from 16KD to 55KD. The isolated IC showed antibodies reacting to 16KD DNA. In the isolated IC, DNAs with molecules less than 300 base pairs were detected. These DNA were double-stranded and hybridized with nuclear DNAs originated from human but not from other species.
Okubo
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Y
Second Department of Internal Medicine, Fukusima Medical College.
D000936:Antigen-Antibody Complex / Q000032:analysis*; D004247:DNA / Q000032:analysis*; D006801:Humans; D007128:Immunoglobulin Fragments / Q000032:analysis*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*
D000936:Antigen-Antibody Complex; D007128:Immunoglobulin Fragments; D004247:DNA