Effect of PAF on erythrocyte membrane heterogeneity: a fluorescence study.
The effect of platelet activating factor, PAF, on the erythrocyte membrane heterogeneity was investigated by measuring the steady-state fluorescence anisotropy and fluorescence decay of 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) incorporated in hemoglobin-free erythrocyte membranes. PAF induced a significant increase of the lipid order in the exterior part of the membrane and a decrease in membrane heterogeneity. These results indicate that PAF may cause changes in the physico-chemical structure of the erythrocyte membrane.
Kantar
A
A
Department of Pediatrics, University of Ancona, Italy.
Giorgi
P L
PL
Curatola
G
G
Fiorini
R
R
D004161:Diphenylhexatriene / Q000031:analogs & derivatives; D004910:Erythrocyte Membrane / Q000502:physiology* / Q000648:ultrastructure; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D006801:Humans; D010972:Platelet Activating Factor / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D010972:Platelet Activating Factor; D004161:Diphenylhexatriene; C033628:1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene