title: Electron spectroscopic diffraction and imaging of the early and mature stages of calcium phosphate formation in the epiphyseal growth plate.
abstract: A review of different models of biomineralization in collagen-rich hard tissues shows that further investigations of crystal formation are necessary. The electron spectroscopic diffraction (ESD) mode of operation of an energy-filtering electron microscope offers the possibility of being able to avoid the background from inelastic scattering in selected-area electron diffraction patterns. First experiments on the different stages of mineralization in the epiphyseal growth plate have only indicated the presence of apatite. The ESD mode can be complemented by the electron spectroscopic imaging mode and by elemental mapping of calcium.
authors.affiliation: Institut für Medizinische Physik, Universität Münster, Germany.
authors.forename: R H
authors.initials: RH
authors.lastname: Barckhaus
authors.forename: H J
authors.initials: HJ
authors.lastname: Höhling
authors.forename: I
authors.initials: I
authors.lastname: Fromm
authors.forename: P
authors.initials: P
authors.lastname: Hirsch
authors.forename: L
authors.initials: L
authors.lastname: Reimer
mesh_terms: D000818:Animals; D001031:Apatites / Q000737:chemistry; D002118:Calcium / Q000032:analysis; D002130:Calcium Phosphates / Q000737:chemistry*; D003460:Crystallization; D003461:Crystallography; D003720:Densitometry; D006132:Growth Plate / Q000378:metabolism*; D008854:Microscopy, Electron; D013552:Swine; D014961:X-Ray Diffraction
chemical_list: D001031:Apatites; D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous; C494366:calcium phosphate, dibasic, dihydrate; D002118:Calcium