Induction of differentiation of human leukemia cells by inhibitors of myosin light chain kinase.
Inhibitors of myosin light chain kinase, 1-(5-chloronaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine hydrochloride (ML-9) and 1-(5-iodonaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine hydrochloride (ML-7), induced Nitroblue tetrazolium reducing activity, lysozyme activity and morphological maturation of human monoblastic U937, THP-1 and promyelocytic HL-60 cells, but not of erythroblastic K562 cells. However, three analogs of ML-9, which are an inhibitor and an activator of protein kinase C, and a calmodulin antagonist, respectively, did not induce differentiation of the cells.
Makishima
M
M
Saitama Cancer Center, Japan.
Honma
Y
Y
Hozumi
M
M
Sampi
K
K
Hattori
M
M
Motoyoshi
K
K
D001381:Azepines / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology; D007948:Leukemia, Monocytic, Acute / Q000473:pathology; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology; D009113:Muramidase / Q000378:metabolism; D009219:Myosin-Light-Chain Kinase / Q000037:antagonists & inhibitors*; D009281:Naphthalenes / Q000494:pharmacology*; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010084:Oxidation-Reduction; D014407:Tumor Cells, Cultured
D001381:Azepines; D009281:Naphthalenes; C056218:ML 9; C070571:ML 7; D009580:Nitroblue Tetrazolium; D009219:Myosin-Light-Chain Kinase; D009113:Muramidase