title: Autoradiographic analysis of diaminopimelic acid incorporation in filamentous cells of Escherichia coli: repression of peptidoglycan synthesis around the nucleoid.
abstract: Peptidoglycan synthesis rate in nonconstricting filaments of Escherichia coli dnaX(Ts) has been studied by autoradiography of incorporated [3H]diaminopimelic acid. Analysis of autoradiograms of whole cells and sacculi showed that peptidoglycan is synthesized at a reduced rate in the nucleoid-containing parts of these filaments. The lower rate of peptidoglycan synthesis in the cell center coincides with a higher local rate of protein synthesis. DNA-less cell formation in dnaX(Ts), dnaX(Ts) sfiA, and the minB minicell-forming mutant is accompanied by a local increase in peptidoglycan synthesis at the constriction site.
authors.affiliation: Department of Molecular Cell Biology, University of Amsterdam, The Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: Mulder
authors.forename: C L
authors.initials: CL
authors.lastname: Woldringh
mesh_terms: D001345:Autoradiography; D001426:Bacterial Proteins / Q000096:biosynthesis; D002473:Cell Wall / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D003960:Diaminopimelic Acid / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D009154:Mutation; D010457:Peptidoglycan / Q000096:biosynthesis*; D012097:Repressor Proteins / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010457:Peptidoglycan; D012097:Repressor Proteins; D003960:Diaminopimelic Acid; D007930:Leucine