Solid-phase gene assembly.
New technologies for multiple chemical synthesis of oligonucleotides and stepwise hybridization on a solid-phase support enable the rapid and cost-effective preparation of long duplex DNA regions. Will these new technologies usher in a new era in protein engineering?
Beattie
K L
KL
Center for Biotechnology, Baylor College of Medicine, The Woodlands, Texas 77381.
Fowler
R F
RF
D004247:DNA / Q000138:chemical synthesis* / Q000235:genetics; D005813:Genes, Synthetic; D005818:Genetic Engineering / Q000379:methods*; D016045:Human Genome Project; D006801:Humans; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis; D015202:Protein Engineering / Q000379:methods
D009841:Oligonucleotides; D004247:DNA