Effect of dietary nucleotides and orotate on the blood levels of prostacyclin (PGI2) and thromboxane (TXA2) in the weanling rat.
Dietary nucleotides affect the maintenance of immune responses, tissue repair and polyunsaturated fatty acid metabolism. Orotate, a pyrimidine nucleotide precursor, induces fatty livers by impairing VLDL hepatic secretion. The aim of this study was to evaluate the changes in the blood levels of fatty acids and prostacyclin (PGI2) and thromboxane (TXA2) in the weanling rat caused by the dietary intake of nucleotides and orotate. Three groups of rats at weaning were fed a control diet, an orotate supplemented diet (O-50) and a nucleotide supplemented diet (N-50) during 4 weeks, respectively. Absolute values of plasma polyunsaturated fatty acids greater than 18 carbon atoms of the n-6 and n-3 series were increased in the N-50 group and decreased in O-50 with regard to the control. However, the relative fatty acid composition of plasma lipid fractions was mostly unaffected. Plasma 6-keto-PGF1 alpha showed a trend to be increased in N-50 and serum TXB2 was significantly increased in that group. Both eicosanoids were unchanged by dietary orotate intake. These results may be explained because of the increased plasma 20:4n-6 found in rats fed a supplemented nucleotide diet. Thus, nucleotides present in foods appear to modulate PUFA conversion and eicosanoids synthesis in early life.
Ramírez
M
M
Departmento de Investigaciòn de UNIASA, Granada, Spain.
Hortelano
P
P
Boza
J
J
Jiménez
J
J
Gil
A
A
Pita
M L
ML
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000284:Administration, Oral; D000818:Animals; D004032:Diet; D004041:Dietary Fats / Q000378:metabolism; D015777:Eicosanoids / Q000096:biosynthesis; D011464:Epoprostenol / Q000097:blood*; D005231:Fatty Acids, Unsaturated / Q000097:blood*; D008079:Lipoproteins, VLDL / Q000096:biosynthesis; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009711:Nucleotides / Q000008:administration & dosage / Q000494:pharmacology*; D009963:Orotic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D013928:Thromboxane A2 / Q000097:blood*; D013929:Thromboxane B2 / Q000097:blood
D004041:Dietary Fats; D015777:Eicosanoids; D005231:Fatty Acids, Unsaturated; D008079:Lipoproteins, VLDL; D009711:Nucleotides; D013929:Thromboxane B2; D013928:Thromboxane A2; D015121:6-Ketoprostaglandin F1 alpha; D009963:Orotic Acid; D011464:Epoprostenol