Complementary DNA sequence of rabbit CAP18--a unique lipopolysaccharide binding protein.
CAP18 is a novel 18 kDa cationic protein [pI approximately 10] originally purified from rabbit granulocytes using as an assay the agglutination of lipopolysaccharide (LPS) coated erythrocytes. cDNA clones encoding CAP18 were isolated from a rabbit bone marrow cDNA library using a PCR generated oligonucleotide probe derived from the N-terminal amino acid sequence. The deduced amino acid sequence reveals a putative signal sequence of 29 amino acids and a mature protein of 142 amino acid residues. The predicted size of the encoded protein is 16.6 kDa with a pI of 10. There are no N-linked glycosylation sites. The CAP18 sequence bears no homology with other known LPS-binding proteins including human bacterial permeability increasing protein (BPI)(1) and rabbit LPS binding protein (LBP)(2).
Larrick
J W
JW
Genelabs Incorporated, Redwood City, CA 94063.
Morgan
J G
JG
Palings
I
I
Hirata
M
M
Yen
M H
MH
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides; D001483:Base Sequence; D015152:Blotting, Northern; D001853:Bone Marrow / Q000502:physiology; D002352:Carrier Proteins / Q000235:genetics*; D054804:Cathelicidins; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D011817:Rabbits
D023181:Antimicrobial Cationic Peptides; D002352:Carrier Proteins; D054804:Cathelicidins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D004247:DNA