Toxicological screening of drugs by microbore high-performance liquid chromatography with photodiode-array detection and ultraviolet spectral library searches.
We use ultraviolet data, acquired with a photodiode-array detector coupled to a reversed-phase liquid-chromatographic system, to identify unknown drugs in plasma samples of acutely poisoned patients. Both retention time and spectra of the peaks obtained with a microbore Hypersil ODS column under gradient elution are compared with a library of approximately 350 compounds. We present our three-year experience with this system, which identifies drugs in less than 1 h, with a high degree of confidence.
Turcant
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Laboratoire de Pharmacologie, Centre Hospitalier Universitaire, Angers, France.
Premel-Cabic
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Cailleux
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Allain
P
P
D002851:Chromatography, High Pressure Liquid; D003627:Data Interpretation, Statistical; D006801:Humans; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011041:Poisoning / Q000175:diagnosis*; D013056:Spectrophotometry, Ultraviolet
D004364:Pharmaceutical Preparations