title: Detection of the illegal use of ethinylestradiol in cattle urine by gas chromatography-mass spectrometry.
abstract: A method for the detection of ethinylestradiol in cattle urine is described, based on enzymic hydrolysis of the sample, clean-up by means of disposable octadecyl and amino solid-phase extraction columns, fractionation by reversed-phase high-performance liquid chromatography, and detection by gas chromatography-mass spectrometry (selected-ion monitoring). Identification is based on both gas chromatographic and mass spectrometric data. The method has been tested on urine samples for a collaborative study and all the results found were correct.
authors.affiliation: Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, State University of Ghent, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Daeseleire
authors.forename: A
authors.initials: A
authors.lastname: De Guesquière
authors.forename: C
authors.initials: C
authors.lastname: Van Peteghem
mesh_terms: D000818:Animals; D002417:Cattle / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004997:Ethinyl Estradiol / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D015813:Substance Abuse Detection
chemical_list: D004997:Ethinyl Estradiol