Multiple trans-splicing events are required to produce a mature nad1 transcript in a plant mitochondrion.
The mitochondrial gene encoding NADH dehydrogenase subunit 1 (nad1) in Petunia hybrida is split into five exons, a, b, c, d, and e. With the use of a complete restriction map of the 443-kb Petunia mitochondrial genome, we have cloned these exons and mapped their location. Exon a is located 130 kb away from and in the opposite orientation from exons b and c. Exon d maps 95 kb away and in the opposite orientation from exons b and c. Exons d and e are separated by 190 kb. By performing the polymerase chain reaction on Petunia cDNAs, we have shown that transcripts from these five exons are joined via a series of cis- and trans-splicing events to create a mature nad1 transcript. In addition, we have found 23 C----U RNA edit sites in Petunia nad1. RNA editing changes 19 of the amino acids predicted by the genomic sequence.
Conklin
P L
PL
Section of Genetics and Development, Cornell University, Ithaca, New York 14853.
Wilson
R K
RK
Hanson
M R
MR
D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D009690:Nucleic Acid Conformation; D015345:Oligonucleotide Probes; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D012326:RNA Splicing; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D004272:DNA, Mitochondrial; D015345:Oligonucleotide Probes; D009245:NADH Dehydrogenase