title: Technetium-99m-d,1-hexamethylpropyleneamine oxime (HMPAO) uptake and glutathione content in brain tumors.
abstract: Technetium-d, HMPAO SPECT was performed in 70 patients suffering from intracerebral tumors of various histologic types (glioma n = 30, meningioma n = 19, metastases n = 10, angioma n = 3, neuroma n = 2, lymphoma n = 2, neurocytoma n = 1, epidermoid n = 1, gliosis n = 1, cholesteatoma n = 1). Tumor classification was histologically verified in all subjects except in two cases with inoperable angiomas. SPECT was performed under resting state conditions with a dual-head rotating camera (SIEMENS ZLC 37) following intravenous injection of 18-25 mCi 99mTc-d, 1-HMPAO. Regional tracer deposit was expressed in terms of a cerebellar index (CBI). Significantly higher regional HMPAO uptake was found in meningiomas when compared with gliomas of different malignancy (ANOVA p less than 0.05). Within gliomas, regional uptake increased with malignancy (n.s.). In 23 patients, a total of 32 tumor specimens were obtained for histochemical analysis of glutathione (GSH) content using high-pressure liquid chromatography. A significant correlation (least square method, p less than 0.001) between CBIs and GSH values was found, supporting the hypothesis that GSH is the predominant factor for the conversion of the lipophilic complex to hydrophilic derivates.
authors.affiliation: Department of Biochemical Pharmacology, University of Vienna, Austria.
authors.forename: E
authors.initials: E
authors.lastname: Suess
authors.forename: S
authors.initials: S
authors.lastname: Malessa
authors.forename: K
authors.initials: K
authors.lastname: Ungersböck
authors.forename: P
authors.initials: P
authors.lastname: Kitz
authors.forename: I
authors.initials: I
authors.lastname: Podreka
authors.forename: K
authors.initials: K
authors.lastname: Heimberger
authors.forename: O
authors.initials: O
authors.lastname: Hornykiewicz
authors.forename: L
authors.initials: L
authors.lastname: Deecke
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001932:Brain Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000556:secondary; D002560:Cerebrovascular Circulation / Q000502:physiology; D005260:Female; D005910:Glioma / Q000000981:diagnostic imaging / Q000378:metabolism; D005978:Glutathione / Q000502:physiology*; D006391:Hemangioma / Q000000981:diagnostic imaging / Q000378:metabolism; D006801:Humans; D008297:Male; D008577:Meningeal Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism; D008579:Meningioma / Q000000981:diagnostic imaging / Q000378:metabolism; D008875:Middle Aged; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics*; D010091:Oximes / Q000493:pharmacokinetics*; D019690:Technetium Tc 99m Exametazime; D015899:Tomography, Emission-Computed, Single-Photon
chemical_list: D015609:Organotechnetium Compounds; D010091:Oximes; D019690:Technetium Tc 99m Exametazime; D005978:Glutathione