title: Trace analysis of N-nitrosoureas by their alkylating activity.
abstract: N-Nitrosoureas can be analysed indirectly by gas chromatography with chemoluminescence detection after reaction of the corresponding diazoalkanes with the scavenger reagent N-nitroso-N-tert-butylglycine. A lower determination limit of 2 ng N-methyl-N-nitrosourea per sample is achieved. The method is specific for N-nitroso compounds, which release nonpolar diazoalkanes upon alkaline treatment.
authors.affiliation: German Cancer Research Centre, Institute of Toxicology and Chemotherapy, Heidelberg.
authors.forename: P
authors.initials: P
authors.lastname: Mende
authors.forename: B
authors.initials: B
authors.lastname: Spiegelhalder
authors.forename: R
authors.initials: R
authors.lastname: Preussmann
mesh_terms: D000478:Alkylation; D002849:Chromatography, Gas; D009607:Nitrosourea Compounds / Q000032:analysis*
chemical_list: D009607:Nitrosourea Compounds