[Hematolymphoid system in the liver--restoring mechanism of hemopoiesis in the regenerating liver].
For the study of immunological phenomena peculiar to liver transplantation, it would be important to know well hematolymphoid system in the liver. In this paper, we report the restoring mechanism of hemopoiesis in the murine regenerating liver. 1) We found hemopoietic stem cells (HSC) and wheat germ agglutinin positive cells (WAG-PC) in both fractions of peripheral blood lymphocytes (PBL) and intrahepatic lymphocytes (IHL) from untreated C3H/He mice. The numbers of HSC and WGA-PC in each fraction gradually increased after partial hepatectomy. This change was remarkable in the population of IHL, associated more strongly with the liver. Colonies, which were generated from IHL in a fibrin clot culture system, are mainly composed of granulocytes, macrophages and mast cells. 2) We found colony-stimulating activity not only in the culture supernatant of normal nonparenchymal liver cells but in that of normal parenchymal liver cells. Furthermore, we clarified that one of colony-stimulating factors in both culture supernatants is GM-CSF. This result suggests parenchymal liver cells also release cytokines such as GM-CSF. These facts show that hematolymphoid system in the liver is a complicated network, composed of parenchymal liver cells, nonparenchymal liver cells, hemopoietic stem cells, and other cell populations.
Sakamoto
T
T
Second Department of Surgery, Nippon Medical School, Tokyo, Japan.
Shoji
T
T
Yokomuro
K
K
D000818:Animals; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000032:analysis; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells; D006498:Hepatectomy; D008099:Liver / Q000502:physiology*; D008115:Liver Regeneration / Q000502:physiology*; D051379:Mice; D008809:Mice, Inbred C3H
D003115:Colony-Stimulating Factors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor