Effect of cigarette smoking on the levels of platelet-activating factor-like lipid(s) in plasma lipoproteins.
The effect of cigarette smoking on the levels of platelet-activating factor-like lipid(s) (PAF-LL) in plasma lipoproteins was studied. The subjects were 10 healthy male non-smokers (24 +/- 1.4 years old) and 13 healthy male habitual smokers (23 +/- 1.3 years old). Fasting venous blood was obtained and basal levels of PAF-LL in plasma lipoproteins were estimated. The acute effect of cigarette smoking was also studied in smokers. Plasma lipoproteins were separated by ultracentrifugation. Lipids were extracted and separated by thin-layer chromatography. The fraction with the same migration as authentic PAF was recovered and was shown to cause aggregation of human polymorphonuclear neutrophils. This activity was identified as PAF-LL because it was inactivated by phospholipase A2 and was blocked by CV-3988, an antagonist of the PAF receptor. PAF-LL was detected in LDL and HDL, but not in VLDL or in lipoprotein-deficient plasma. The levels of PAF-LL in LDL in non-smokers, and in smokers before and after smoking were 13 +/- 7.5, 16 +/- 14.9 and 190 +/- 179.0 pg/ml, and those in HDL were 12 +/- 5.2, 40 +/- 40.0 and 235 +/- 205.1 pg/ml, respectively. The values in both LDL and HDL in smokers increased significantly after smoking (P less than 0.05). After 30 min, the levels had returned almost to the pre-smoking levels. We conclude that cigarette smoking induces an increase in the levels of PAF or closely related lipid(s) in LDL and HDL, which may be related to smoking-induced atherosclerosis.
Imaizumi
T
T
Department of Pathologic Physiology, Hirosaki University School of Medicine, Japan.
Satoh
K
K
Yoshida
H
H
Kawamura
Y
Y
Hiramoto
M
M
Takamatsu
S
S
D000328:Adult; D002263:Carboxyhemoglobin / Q000032:analysis; D002449:Cell Aggregation / Q000187:drug effects; D006801:Humans; D008055:Lipids / Q000032:analysis / Q000097:blood* / Q000494:pharmacology; D008074:Lipoproteins / Q000032:analysis / Q000097:blood*; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology; D010972:Platelet Activating Factor / Q000032:analysis* / Q000494:pharmacology; D012907:Smoking / Q000097:blood*; D013997:Time Factors
D008055:Lipids; D008074:Lipoproteins; D010972:Platelet Activating Factor; D002263:Carboxyhemoglobin