In vitro effect of transforming growth factor-beta on progression of HIV-1 infection in primary mononuclear phagocytes.
In vitro-differentiated monocytes can be infected with the monocytotropic isolate of HIV-1/ADA. The infection is characterized by formation of giant cells and production of virus that can be found in cell supernatants or cell-associated. In this study, we demonstrate that the above described parameters of infection can be enhanced by a factor present in acidified M phi supernatants, suggesting that it might be transforming growth factor beta-1 (TGF-beta 1). When recombinant or purified TGF-beta were examined, similar activities were detected. This effect apparently is not because of changes in the cellular phenotype that could favor infection. The effect of TGF-beta is exerted on cells once infection is established or on cells with active virus production. The activity can be also demonstrated using U-937 cells.
Lazdins
J K
JK
CIBA-GEIGY Ltd., Pharma Research, Basel, Switzerland.
Klimkait
T
T
Woods-Cook
K
K
Walker
M
M
Alteri
E
E
Cox
D
D
Cerletti
N
N
Shipman
R
R
Bilbe
G
G
McMaster
G
G
D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects
D016212:Transforming Growth Factor beta