Discrepancy between biochemical normalization and morphological recovery of jejunal mucosa during postischemic reperfusion in presence of the xanthine oxidase inhibitor oxypurinol.
An increased formation of oxygen free radicals in the reperfused rat small intestine is concluded from accumulations of oxidized glutathione, of thiobarbituric acid-reactive substances and of 4-hydroxynonenal. Xanthine oxidase inhibition prevented these biochemical changes. The histological and electronmicroscopic studies of intestinal sucosa showed significant structural deteriorations already at the end of the ischemic period obviously due to disturbances of cellular energy metabolism. The extent of dosage was increased during the reperfusion without qualitative changes of the pattern of structural dosage. The beneficial effects of oxypurinol on biochemical criteria which occurred already in the early phase of reperfusion were not reflected in significant morphological differences within the first hour of reperfusion. Differences of morphological findings between oxypurinol-treated and untreated animals could be observed after longer periods of reperfusion--during the regeneration of the tissue.
Siems
W
W
Institut für Biochemie, Bereich Medizin (Charité), der Humboldt-Universität zu Berlin, Federal Republic of Germany.
Kowalewski
J
J
David
H
H
Grune
T
T
Bimmler
M
M
D000818:Animals; D005609:Free Radicals; D005978:Glutathione / Q000378:metabolism; D007413:Intestinal Mucosa / Q000098:blood supply* / Q000378:metabolism / Q000648:ultrastructure; D007511:Ischemia / Q000378:metabolism* / Q000473:pathology; D007583:Jejunum / Q000098:blood supply* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D010117:Oxypurinol / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology
D005609:Free Radicals; D010117:Oxypurinol; D005978:Glutathione