Ischemia-reperfusion does not affect reactivity of isolated canine basilar artery.
The effect of ischemia-reperfusion on endothelium-dependent relaxations and reactivity of vascular smooth-muscle cells was studied in rings of basilar arteries obtained from six dogs exposed to 12 min of complete global cerebral ischemia followed by 100 min of reperfusion. Three sham-operated control dogs served as controls. Ischemia was induced either by an increase in intracranial pressure or by aortic occlusion. The rings were suspended for isometric tension recording in physiological salt solution. Ischemia-reperfusion did not affect endothelium-dependent relaxations to vasopressin and bradykinin. In rings without endothelium relaxations to sodium nitroprusside, molsidomine (SIN-1), and papaverine as well as contractions to 5-hydroxytryptamine and KCl were preserved. These results demonstrate that in large canine cerebral arteries, ischemia-reperfusion of these durations does not affect relaxations mediated by activation of endothelium or direct relaxations and contractions of vascular smooth-muscle cells.
Katusić
Z S
ZS
Department of Anesthesiology, Mayo Clinic, Rochester, Minnesota 55905.
Michenfelder
J D
JD
Milde
J H
JH
D000818:Animals; D001488:Basilar Artery / Q000187:drug effects / Q000503:physiopathology*; D001920:Bradykinin / Q000494:pharmacology; D002545:Brain Ischemia / Q000503:physiopathology*; D002560:Cerebrovascular Circulation; D004285:Dogs; D004727:Endothelium; D011189:Potassium Chloride / Q000494:pharmacology; D015424:Reperfusion; D012701:Serotonin / Q000494:pharmacology; D014544:Uridine Triphosphate / Q000494:pharmacology; D014661:Vasoconstriction / Q000187:drug effects; D014667:Vasopressins / Q000494:pharmacology
D014667:Vasopressins; D012701:Serotonin; D011189:Potassium Chloride; D001920:Bradykinin; D014544:Uridine Triphosphate