[Cytoplasmic RNA in neocortical neurons in vitro].
The investigation of the ratio of neuronal cytoplasmic RNA mono- and two-chain sections was performed in thin neocortical slices by means of fluorescent probing. Changes of the relative intensity of Acridine Orange red luminescence indicated a decrease in the quota of monochain sections in the common neuronal RNA pool. In eight hours of superfusion it was authentically less than in the control slices and in the slices which were incubated during four hours. Deep profiles of red fluorescence became plane in the process of incubation. It was suggested that the essential decrease in the red luminescence intensity of cytoplasmic RNA in vitro, observed between four and eight hours, may be conditioned by disintegration of polysomes to monosomes, and this, in its turn, may cause the decrease in ribosomal synthetic activity.
Karnup
S V
SV
Gordon
R Ia
RIa
Iakovenko
G V
GV
Bakharev
B V
BV
D000165:Acridine Orange; D000818:Animals; D001923:Brain Chemistry; D002470:Cell Survival / Q000502:physiology; D046508:Culture Techniques; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism*; D006168:Guinea Pigs; D008163:Luminescent Measurements; D015591:Microspectrophotometry; D009474:Neurons / Q000737:chemistry / Q000378:metabolism*; D010296:Parietal Lobe / Q000737:chemistry / Q000378:metabolism*; D012330:RNA, Double-Stranded / Q000032:analysis / Q000378:metabolism; D012335:RNA, Ribosomal / Q000032:analysis / Q000378:metabolism*; D012270:Ribosomes / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors
D012330:RNA, Double-Stranded; D012335:RNA, Ribosomal; D000165:Acridine Orange
Sostoianie tsitoplazmaticheskoĭ RNK v neĭronakh neokorteksa in vitro.