An immunoelectron microscopic comparison of desmosomal constituents and hemidesmosomal ones originating from the same tissue of the same animal.
The molecular constituents of desmosomes and hemidesmosomes were compared by examining bovine muzzle epidermis under immunoelectron microscopy using a postembedding method, first with antibodies prepared to four desmosomal antigens (DP1/2, DP3, DG1, DG2/3), followed by protein A-gold (PAG) complexes. The four antibodies showed almost negative labeling at hemidesmosomes as compared with the labeling observed at the desmosomes in the same tissue. By counting the number of PAG particles/200 millimicrons at hemidesmosomes and desmosomes, the above qualitative observation was confirmed quantitatively. These results support a new concept which has recently been proposed by several researchers that hemidesmosomes and desmosomes are immunochemically distinct.
Shida
H
H
Department of Biology, University of Yamanashi Medical School, Japan.
Steinberg
M S
MS
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology; D002417:Cattle; D003896:Desmosomes / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D004817:Epidermis / Q000648:ultrastructure; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D006046:Gold; D007150:Immunohistochemistry / Q000379:methods; D016253:Microscopy, Immunoelectron; D013205:Staphylococcal Protein A
D000906:Antibodies; D000941:Antigens; D006023:Glycoproteins; D013205:Staphylococcal Protein A; D006046:Gold