Dissociation of photoreceptor cells from the pineal organ of the lamprey, Lampetra japonica.
Photoreceptor cells, nerve cells and supporting cells were dissociated from the pineal organ of the river lamprey, Lampetra japonica, by the use of 10 U/ml papain solution at 28 degrees C for 20 min, followed by repeated trituration. With the aid of Nomarski interference-contrast optics, photoreceptor cells, nerve cells and supporting cells were readily identified. Electron-microscopic examination revealed that isolated photoreceptor cells display an outer segment endowed with a few lamellar disks and connected to the inner segment (ellipsoid) via a connecting cilium. The structural features of the dissociated photoreceptor and supporting cells strongly resemble the morphology of the respective cellular elements in situ. We succeeded in culturing dissociated cells for time periods up to 48 h when the procedure described in detail was applied.
Samejima
M
M
First Department of Physiology, Hamamatsu University School of Medicine, Japan.
Tamotsu
S
S
Muranaka
Y
Y
Morita
Y
Y
D000818:Animals; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D007798:Lampreys / Q000033:anatomy & histology*; D008854:Microscopy, Electron; D008857:Microscopy, Interference; D010786:Photoreceptor Cells / Q000166:cytology* / Q000648:ultrastructure; D010870:Pineal Gland / Q000166:cytology* / Q000648:ultrastructure