Identification of inducible damage-recognition proteins that are overexpressed in HeLa cells resistant to cis-diamminedichloroplatinum (II).
Two cis-diamminedichloroplatinum (II) (cisplatin)-inducible proteins [b 130 (approximately 130 kDa) and b95 (approximately 95 kDa]) in HeLa cells that recognize both the cisplatin-modified and u.v.-modified DNA were identified in this study. These damage-recognition proteins were overexpressed in cisplatin-resistant HeLa cells. The results suggest that the damage-recognition proteins are regulated in the cells in response to DNA damage, and they may be important for DNA repair and probably the emergence of cisplatin resistance.
Chao
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CC
Department of Biochemistry, Chang Gung Medical College, Taoyuan, Taiwan, Republic of China.
Huang
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Lee
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Lin-Chao
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D015139:Blotting, Southern; D015153:Blotting, Western; D002945:Cisplatin / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D004351:Drug Resistance; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008970:Molecular Weight; D014466:Ultraviolet Rays
D004268:DNA-Binding Proteins; D004247:DNA; D002945:Cisplatin