Determination of [3H]brifentanil, a potent narcotic analgesic, from rat serum by high-performance liquid chromatography with on-line radioactive detection.
A method for rapid analysis of [3H]brifentanil extracted from rat serum is described that has the advantages of sensitivity, speed and specificity. The method is based on extraction from serum via solid-phase extraction followed by chromatographic separation on a reversed-phase high-performance liquid chromatographic column. Detection of [3H]brifentanil is accomplished with an on-line radioactive detector, thus the laborious step of peak collection and subsequent liquid scintillation counting is eliminated. The developed method is sensitive to 0.1 ng/ml and has been successfully applied to pharmacokinetic studies in rats. In vivo metabolites retaining the radiolabel have been detected with the method and were more polar than the parent compound as based upon the elution order on the reversed-phase system.
Kvalo
L T
LT
Pharmaceutical Research Department, Anaquest Inc., Murray Hill, NJ 07974.
Wilhelm
J A
JA
Edfort
M J
MJ
Venturella
V S
VS
D000700:Analgesics / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005283:Fentanyl; D010880:Piperidines / Q000097:blood*; D051381:Rats; D013777:Tetrazoles / Q000097:blood*; D014316:Tritium
D000700:Analgesics; D010880:Piperidines; D013777:Tetrazoles; D014316:Tritium; C065913:Brifentanil; D005283:Fentanyl