title: Effect of vitamin B2 deficiency on rat liver dihydropyrimidine dehydrogenase activity.
abstract: Effect of vitamin B2 deficiency on rat liver dihydropyrimidine dehydrogenase was investigated. It was found that the purified enzyme contains 2 mol flavin per molecule, which consists of equal proportions of flavin-adenine dinucleotide (FAD) and riboflavin 5'-phosphate (FMN). When rats were fed on a vitamin B2-deficient diet for 5 weeks, dihydropyrimidine dehydrogenase activity in the liver was diminished, followed by a decrease in enzyme concentration. Moreover, the addition of exogenous FAD or FMN did not restore the activity. Thus endogenous flavin may regulate the enzyme half-life or synthesis. Lowering of dihydropyrimidine dehydrogenase activity in the livers of rats fed on a vitamin B2-deficient diet did not affect the uridine, uracil and sigma UMP (the sum of acid soluble uracil 5'-nucleotides) pool in liver.
authors.affiliation: Laboratory of Nutritional Chemistry, Faculty of Nutrition, Kobe-Gakuin University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Fujimoto
authors.forename: K
authors.initials: K
authors.lastname: Matsuda
authors.forename: M
authors.initials: M
authors.lastname: Kikugawa
authors.forename: M
authors.initials: M
authors.lastname: Kaneko
authors.forename: N
authors.initials: N
authors.lastname: Tamaki
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D042943:Dihydrouracil Dehydrogenase (NADP); D005415:Flavins / Q000032:analysis / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D010088:Oxidoreductases / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000302:isolation & purification; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D012257:Riboflavin Deficiency / Q000201:enzymology*
chemical_list: D005415:Flavins; D010088:Oxidoreductases; D042943:Dihydrouracil Dehydrogenase (NADP)