Repair of damage caused by UV- and X-irradiation in the amoeboflagellate Naegleria gruberi.
Cysts of Naegleria gruberi have a normal UV- and an extremely high X-ray resistance compared to other protozoans. Caffeine and 3-aminobenzamide applied to excysting amoeba after irradiation in the encysted state (UV and X-rays) by feeding with drug-containing bacteria increased lethality, while fractionated irradiation (UV) and liquid-holding (UV and X-rays) increased survival. Illumination with visible light after UV-irradiation restored almost 100% viability. The results are discussed in regard to the activity of repair mechanisms.
Hillebrandt
S
S
Institut für Entwicklungsphysiologie der Universität zu Köln, Federal Republic of Germany.
Müller
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I
D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002110:Caffeine / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D004260:DNA Repair; D007700:Kinetics; D009253:Naegleria / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology; D014466:Ultraviolet Rays; D014965:X-Rays
D001549:Benzamides; D011838:Radiation-Sensitizing Agents; D002110:Caffeine; C025160:3-aminobenzamide