A monoclonal antibody recognizing a common antigen on Drosophila embryos and human fibroblasts.
We used a monoclonal antibody specific for vimentin from human fibroblasts to stain whole mounts of Drosophila embryos. In immunofluorescence observations this antibody cross-reacts with an antigenic determinant localized throughout mitosis at the nuclear boundary. Double fluorescence observations with the Rb188 antibody that specifically recognizes a centrosomal protein of the Drosophila embryo [Whitfield et al., 1988] showed that the anti-vimentin antibody cross-reacts with an antigen localized in the centrosomal region.
Callaini
G
G
Department of Evolutionary Biology, University of Siena, Italy.
Riparbelli
M G
MG
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D003429:Cross Reactions / Q000276:immunology; D004330:Drosophila / Q000196:embryology / Q000276:immunology*; D005347:Fibroblasts / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D014746:Vimentin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D014746:Vimentin