title: Dietary glucose increases plasma insulin and decreases brown adipose tissue thermogenic activity in adrenalectomized ob/ob mice.
abstract: The purpose of this study was to determine whether consumption of a high glucose diet would increase plasma insulin concentrations and decrease brown adipose tissue metabolism in adrenalectomized ob/ob mice previously fed a high starch diet. Male sham-operated and adrenalectomized ob/ob and lean mice were fed a high starch diet for 12 d, then switched to a high glucose diet for the last 2 or 4 d of the 14- or 16-d feeding trials. Adrenalectomized ob/ob mice consumed 16% more energy and gained 50% more weight without an increase in oxygen consumption when switched from a high starch diet to a high glucose diet. Within 2 d after the switch to the high glucose diet, plasma insulin concentrations increased by 70% without any change in plasma glucose concentrations; brown adipose tissue metabolism, as assessed by GDP binding to brown adipose tissue mitochondria, was decreased by 26% 4 d after the diet switch. Sham-operated ob/ob and lean mice and adrenalectomized lean mice were minimally affected by the switch to the high glucose diet. The increase in plasma insulin concentrations in adrenalectomized ob/ob mice induced by the high glucose diet may contribute to the observed depression in brown adipose tissue metabolism.
authors.affiliation: Department of Food Science and Human Nutrition, Michigan State University, East Lansing 48824-1224.
authors.forename: Y M
authors.initials: YM
authors.lastname: Nei
authors.forename: D R
authors.initials: DR
authors.lastname: Romsos
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000284:Administration, Oral; D000315:Adrenalectomy; D000818:Animals; D001833:Body Temperature Regulation; D001835:Body Weight / Q000187:drug effects; D004040:Dietary Carbohydrates / Q000494:pharmacology*; D005502:Food; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology*; D007328:Insulin / Q000097:blood*; D008297:Male; D051379:Mice; D008820:Mice, Obese; D010101:Oxygen Consumption
chemical_list: D004040:Dietary Carbohydrates; D007328:Insulin; D005947:Glucose