Analytical studies on the chiral separation and simultaneous determination of pantothenic acid and hopantenic acid enantiomers in rat plasma by gas chromatography-mass fragmentography.
The chiral separation and simultaneous determination of D- and L-pantothenic acids and D- and L-hopantenic acids in rat plasma using gas chromatography-mass fragmentography are described. The method is based on deproteinization by ion-exchange resin, extraction with ethyl acetate under acidic conditions, and derivatization to form several interesting compounds. After methyl esterification for carboxylic acid of D- and L-pantothenic acids, D- and L-hopantenic acids could be derivatized to trifluoroacetate, cyclic sulphite, and cyclic n-butylboronate for hydroxy groups. D- and L-forms of these derivatives were completely separated by mass fragmentographic technique with quasi-molecular ions. Calcium salt of D-5-[(2,4-dihydroxy-3,3-dimethyl-1-oxobutyl)amino]pentanoic acid was used as an internal standard for the determination of DL-pantothenic acids and DL-hopantenic acids. The detection limits of DL-pantothenic acids and DL-hopantenic acids in this method were 5 and 12 ng, respectively. This method could be applied to the study of plasma levels of D-, L-pantothenic acids and D-, L-hopantenic acids in rat.
Banno
K
K
Analytical Chemistry Research Laboratory, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
Horimoto
S
S
Matsuoka
M
M
D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods* / Q000592:standards; D010205:Pantothenic Acid / Q000031:analogs & derivatives* / Q000097:blood* / Q000302:isolation & purification; D051381:Rats; D013237:Stereoisomerism; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives* / Q000097:blood / Q000302:isolation & purification
C016030:pantogab; D010205:Pantothenic Acid; D005680:gamma-Aminobutyric Acid