title: Modified Western blot assay for confirmation and differentiation of human T cell lymphotropic virus types I and II.
abstract: Disease association studies of human T cell lymphotropic virus (HTLV) types I and II are hindered by the need for multiple assays to confirm and differentiate between the viruses. A modified Western blot assay has been developed using HTLV-I viral lysate and unique (MTA-4) and shared (p21E) HTLV recombinant proteins. By defining confirmation of infection as the presence of antibodies to p24 gag protein and to p21E, all 56 HTLV-I and 49 HTLV-II antisera were confirmed by this modified Western blot alone. Differentiation was determined by reactivity to MTA-4. All HTLV-I antisera reacted with MTA-4 and all HTLV-II antisera did not react with MTA-4. These findings indicate the utility of selected HTLV-I recombinant proteins in a single assay format to confirm and differentiate infections with HTLV-I and HTLV-II.
authors.affiliation: Department of Pathology, Stanford University School of Medicine, Palo Alto, CA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Lipka
authors.forename: P
authors.initials: P
authors.lastname: Santiago
authors.forename: L
authors.initials: L
authors.lastname: Chan
authors.forename: G R
authors.initials: GR
authors.lastname: Reyes
authors.forename: K P
authors.initials: KP
authors.lastname: Samuel
authors.forename: W A
authors.initials: WA
authors.lastname: Blattner
authors.forename: G M
authors.initials: GM
authors.lastname: Shaw
authors.forename: C V
authors.initials: CV
authors.lastname: Hanson
authors.forename: J J
authors.initials: JJ
authors.lastname: Sninsky
authors.forename: S K
authors.initials: SK
authors.lastname: Foung
mesh_terms: D015153:Blotting, Western; D005189:False Positive Reactions; D015481:HTLV-I Antibodies / Q000097:blood; D015490:HTLV-I Infections / Q000175:diagnosis*; D015482:HTLV-II Antibodies / Q000097:blood; D015491:HTLV-II Infections / Q000175:diagnosis*; D015368:Human T-lymphotropic virus 1 / Q000276:immunology / Q000302:isolation & purification*; D015367:Human T-lymphotropic virus 2 / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D015531:Radioimmunoprecipitation Assay
chemical_list: D015481:HTLV-I Antibodies; D015482:HTLV-II Antibodies; D007106:Immune Sera