cDNA cloning, sequence analysis and tissue distribution of a precursor for vasoactive intestinal contractor (VIC).
A full-length cDNA encoding preprovasoactive intestinal contractor (PPVIC) has been cloned. From the deduced 160 amino acid PPVIC, the mature VIC is predicted to be produced via a 37 residue intermediate, big VIC. The PPVIC also contains a VIC-like peptide of 16 amino acids structurally related to to the amino-terminal residues of VIC and flanked by pairs of dibasic amino acids, putative processing sites. RNA blot hybridization with PPVIC cDNA confirmed the PPVIC gene to be expressed in the small and large intestinal tract in a tissue specific manner.
Saida
K
K
Cell Science and Technology Division, Agency of Industrial Science and Technology, Ibaraki, Japan.
Mitsui
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011498:Protein Precursors; C059568:vasoactive intestinal constrictor; D004247:DNA