Alteration of the phase and period of a circadian oscillator by a reversible transcription inhibitor.
A function for transcription in the mechanism of a circadian oscillator was investigated with the reversible transcription inhibitor 5,6-dichloro-1-beta-D- ribobenzimidazole (DRB). Two-hour treatments with DRB shifted the phase of the circadian rhythm of the isolated eye of Aplysia, and continuous treatments of DRB lengthened the free running period of this rhythm. Camptothecin, an inhibitor of transcription that is structurally unrelated to DRB, had similar effects on the circadian rhythm. These results suggest that transcription may be part of the circadian oscillating mechanism.
Raju
U
U
Department of Biochemical and Biophysical Sciences, University of Houston, TX 77204-5934.
Koumenis
C
C
Nunez-Regueiro
M
M
Eskin
A
A
D000818:Animals; D001048:Aplysia; D002166:Camptothecin / Q000494:pharmacology*; D002940:Circadian Rhythm / Q000528:radiation effects*; D004004:Dichlororibofuranosylbenzimidazole / Q000494:pharmacology*; D005123:Eye / Q000187:drug effects; D066298:In Vitro Techniques; D009799:Ocular Physiological Phenomena; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*; D014529:Uridine / Q000378:metabolism
D004004:Dichlororibofuranosylbenzimidazole; D014529:Uridine; D002166:Camptothecin