title: An analysis of the arterial input curve for technetium-99m-HMPAO: quantification of rCBF using single-photon emission computed tomography.
abstract: Arterial radioactivity content after the intravenous administration of HMPAO in seven human subjects was analyzed. Arterial sampling of 99mTc-HMPAO was performed on each subject over a 25-min period postinjection. The lipophilic fraction of the tracer present in the blood was rapidly extracted with octanol. An analysis of the time course of the extracted and nonextracted octanol fractions was performed in order to calculate the arterial input of the tracer available for brain extraction. HMPAO net regional brain clearances were then calculated and compared with rCBF values obtained in the same patients using 99mTc-microspheres injected into the left ventricle of the heart. HMPAO brain clearances were 0.41 +/- 0.01 and 0.27 +/- 0.01 ml/min/g for grey and white matter, respectively. Linear regression analysis was performed and the following result was obtained: clearance (HMPAO) = 0.07 + 0.43 . rCBF with a high significance (p less than 0.001). This equation can be used for the transformation of HMPAO clearances into rCBF values. Our study demonstrates that by using HMPAO and SPECT it is possible to obtain a quantitative estimate of rCBF in humans.
authors.affiliation: Dipartimento di Fisiopatologia Clinica, U.O. Cardiologia, USL 10/D Firenze, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Pupi
authors.forename: M T
authors.initials: MT
authors.lastname: De Cristofaro
authors.forename: L
authors.initials: L
authors.lastname: Bacciottini
authors.forename: D
authors.initials: D
authors.lastname: Antoniucci
authors.forename: A R
authors.initials: AR
authors.lastname: Formiconi
authors.forename: M
authors.initials: M
authors.lastname: Mascalchi
authors.forename: U
authors.initials: U
authors.lastname: Meldolesi
mesh_terms: D000328:Adult; D000368:Aged; D001921:Brain / Q000000981:diagnostic imaging*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D006801:Humans; D008297:Male; D008863:Microspheres; D008875:Middle Aged; D015609:Organotechnetium Compounds; D010091:Oximes; D012044:Regression Analysis; D013668:Technetium Tc 99m Aggregated Albumin; D019690:Technetium Tc 99m Exametazime; D013997:Time Factors; D015899:Tomography, Emission-Computed, Single-Photon
chemical_list: D015609:Organotechnetium Compounds; D010091:Oximes; D013668:Technetium Tc 99m Aggregated Albumin; D019690:Technetium Tc 99m Exametazime