A natural specific intracellular ligand as a reagent for cyclosporine blood level determination.
We isolated and characterized a non-immunoglobulin fraction from calf thymuses which was found very active in binding [3H]labelled cyclosporine (CsA). It contains the natural, intracellular receptor cyclophilin, specifically binding to the active site of the CsA molecule, which may also play a part in the immunosuppression of human immunocompetent cells. This material with a high selectivity could be used, instead of specific monoclonal or polyclonal antibodies, to determine CsA levels in patient's blood.
Hasková
V
V
Institute for Clinical and Experimental Medicine, Praha.
Rozprimová
L
L
Svobodová
J
J
D000591:Amino Acid Isomerases / Q000378:metabolism*; D001665:Binding Sites; D002352:Carrier Proteins / Q000378:metabolism*; D003524:Cyclosporins / Q000097:blood* / Q000378:metabolism; D006801:Humans; D019696:Peptidylprolyl Isomerase; D013950:Thymus Gland / Q000737:chemistry*; D014462:Ultrafiltration
D002352:Carrier Proteins; D003524:Cyclosporins; D000591:Amino Acid Isomerases; D019696:Peptidylprolyl Isomerase