title: The 36K polypeptide synthesized in Newcastle disease virus-infected cells possesses properties predicted for the hypothesized 'V' protein.
abstract: Newcastle disease virus (NDV) virions possess two proteins which react strongly with monoclonal antibody 688 following separation by high resolution two-dimensional (isoelectric focusing/SDS) PAGE and detection by Western blotting. One is the phosphorylated nucleocapsid-associated 53K [P (NAP)] protein, the other comigrates with the 36K protein detected by radiolabelling NDV-infected chick embryo fibroblasts. [35S]Cysteine/[3H]leucine dual-labelling experiments show that the 36K protein is very rich in cysteine compared to the P (NAP) protein. In the Beaudette C strain it comigrates on one-dimensional SDS-polyacrylamide gels with the matrix protein (M); however, it is resolved from the slower migrating M protein from the Ulster strain of NDV. The size, strain-specific isoelectric point, high cysteine content and antigenic relatedness to the P (NAP) protein suggest that the 36K protein is the 'V' protein of NDV, the counterpart of which is found in other Paramyxoviridae.
authors.affiliation: Department of Biochemistry and Genetics, University of Newcastle upon Tyne, U.K.
authors.forename: A C
authors.initials: AC
authors.lastname: Samson
authors.forename: I
authors.initials: I
authors.lastname: Levesley
authors.forename: P H
authors.initials: PH
authors.lastname: Russell
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis; D015153:Blotting, Western; D002213:Capsid / Q000096:biosynthesis / Q000737:chemistry* / Q000276:immunology; D002478:Cells, Cultured; D003545:Cysteine / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005347:Fibroblasts / Q000382:microbiology; D007930:Leucine / Q000032:analysis; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000378:metabolism*; D014758:Viral Core Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000276:immunology; D014764:Viral Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000276:immunology; D014771:Virion / Q000378:metabolism*
chemical_list: C070065:36 kDa V protein, Newcastle disease virus; D000596:Amino Acids; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D014758:Viral Core Proteins; D014764:Viral Proteins; D007930:Leucine; D003545:Cysteine