Heat-killed Salmonella typhi induces the release of prostaglandins and leukotrienes from mouse macrophages.
Mouse macrophages pre-labeled with [3H]arachidonic acid (20:4) were shown to release metabolites generated by the lipoxygenase and cyclo-oxygenase pathways following in vitro addition of heat-killed Salmonella typhi. These metabolites were maximally released after 60-90 min of incubation and consisted of prostaglandins (85%), leukotriene C (6%), di-HETEs, leukotrienes D and E (4%), mono-HETEs (2%) and other metabolites (3%). Of the metabolites generated by the cyclo-oxygenase pathway (prostaglandins), 6-keto PGF1 alpha and PGE2 were generated at a ratio of 1.2 to 1. The significance and importance of these results are discussed.
Pang
T
T
Institute for Advanced Studies, University of Malaya, Kuala Lumpur.
Devi
S
S
Puthucheary
S
S
Pawlowski
N
N
D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D007700:Kinetics; D015289:Leukotrienes / Q000096:biosynthesis*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008813:Mice, Inbred ICR; D010529:Peritoneal Cavity / Q000166:cytology; D011453:Prostaglandins / Q000096:biosynthesis*; D012485:Salmonella typhi / Q000276:immunology*; D015054:Zymosan / Q000494:pharmacology
D015289:Leukotrienes; D011453:Prostaglandins; D015054:Zymosan