[The selection of cold-adapted variants of the influenza viruses H1N1 and H3N2 and their antigenic and genetic characteristics].
As a result of serial passages (42 passages) at low temperatures (26 degrees--28 degrees C) of two influenza H1N1 and H3N2 virus strains stable cold-adapted (ca) variants were produced. Investigations of them showed the ca A/USSR/03/84 (H1N1) variant to have ts-mutations in genes 1, 2, 4, 5, 7, and 8 and the ca A/USSR/215/79 (H3N2) to have ts-mutations in genes 1, 3, 4, 5, 7 and 8. These ca-variants may be recommended as attenuation donors to be used in recombination experiments with epidemic influenza viruses in order to obtain attenuated reassortant candidate vaccine strains.
Podcherniaeva
R Ia
RIa
Döhner
L
L
Medvedeva
T E
TE
Aleksandrova
G I
GI
D000222:Adaptation, Physiological / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D002642:Chick Embryo; D003080:Cold Temperature; D014644:Genetic Variation / Q000235:genetics / Q000276:immunology*; D053118:Influenza A Virus, H1N1 Subtype; D053122:Influenza A Virus, H3N2 Subtype; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000235:genetics / Q000276:immunology; D009154:Mutation / Q000235:genetics / Q000276:immunology; D011995:Recombination, Genetic / Q000235:genetics / Q000276:immunology; D012641:Selection, Genetic; D013696:Temperature; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology
D000956:Antigens, Viral; D007252:Influenza Vaccines; D014613:Vaccines, Attenuated
Selektsiia kholdoadaptirovannykh variantov virusov grippa H1N1 i H3N2, ikh antigennaia i geneticheskaia kharakteristika.