Regulatory elements near the Drosophila hsp 22 gene required for ecdysterone and heat shock induction.
A transient expression assay was used to localize cis-acting DNA regulatory elements near the Drosophila heat shock protein (hsp) 22 gene, that are involved in heat shock expression and in ecdysterone-induced expression. The results identify a region between positions -320 and -232 that is essential for ecdysterone control, but not for heat-induced expression, and a sequence between -199 and -156, which, when deleted, leads to the loss of heat shock induction. To investigate the function of these DNA sequences, transfection-competition experiments were carried out. The evidence suggests that the DNA regulatory sequences identified by transient expression studies contain binding sites for transacting transcription factors.
Rudolph
K
K
Department of Biology, Dartmouth College, Hanover, NH 03755.
Morganelli
C
C
Berger
E M
EM
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA; D004330:Drosophila / Q000235:genetics*; D004441:Ecdysterone / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010957:Plasmids; D012045:Regulatory Sequences, Nucleic Acid; D015534:Trans-Activators / Q000378:metabolism; D014162:Transfection
D006360:Heat-Shock Proteins; D015534:Trans-Activators; D004441:Ecdysterone; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase