Antigen binding diversity of affinity purified autoantibodies against DNA.
Naturally occurring autoantibodies against native DNA (nDNA) in SLE sera showed diverse antigen binding characteristics. The antibodies isolated by affinity chromatography using nDNA linked to Sepharose 4B exhibited specificity towards nDNA and showed strong reactivity with DNA-psoralen photoadduct by direct binding assay and competitive ELISA. The anti-DNA antibody belong to both IgG and IgM immunoglobulin classes and their ratio was 5:1. The possible significance of altered conformation of nDNA in the etiology of SLE has been discussed.
Ali
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Department of Biochemistry, Faculty of Medicine, Aligarh Muslim University.
Hasan
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Ali
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D000974:Antibodies, Antinuclear / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D000916:Antibody Diversity; D000918:Antibody Specificity; D001327:Autoimmune Diseases / Q000276:immunology; D001667:Binding, Competitive; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004247:DNA / Q000276:immunology*; D004277:DNA, Single-Stranded / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005363:Ficusin / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000209:etiology / Q000276:immunology; D009690:Nucleic Acid Conformation; D012685:Sepharose
D000974:Antibodies, Antinuclear; D004277:DNA, Single-Stranded; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D004247:DNA; D012685:Sepharose; D005363:Ficusin