title: The effect of size of polystyrene particles on their retention within the rat peritoneal compartment, and on their interaction with rat peritoneal macrophages in vitro.
abstract: Polystyrene particles (size range 300 nm-3 microns diameter) were radioiodinated and their capture by rat peritoneal macrophages measured in vitro. For unmodified particles, most efficient accumulation was observed using a diameter of 600 nm (Endocytic Index (E.I.) = 16.4 +/- 2.9 microliters/10(6) cells/h). Particles (3 microns diameter) which had been modified to become more hydrophilic by hydroxymethylation showed an increased rate of capture (E.I. = 136.6 +/- 91.2 microliters/10(6) cells/h). Following intraperitoneal administration to rats, unmodified 3 micron particles showed selective accumulation in the omentum (18.4% injected dose/g), and this was increased for the hydroxymethylated bead (35.3% dose/g). The smaller (800 nm) particles were better able to leave the peritoneal compartment. Radiolabelled particles isolated from a peritoneal wash after 5 h were mostly cell-associated (72-86%, depending on the type of particle).
authors.affiliation: Cancer Research Campaign Laboratories, University of Keele, Staffs, UK.
authors.forename: L
authors.initials: L
authors.lastname: Seymour
authors.forename: E
authors.initials: E
authors.lastname: Schacht
authors.forename: R
authors.initials: R
authors.lastname: Duncan
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000378:metabolism*; D004337:Drug Carriers; D066298:In Vitro Techniques; D007274:Injections, Intraperitoneal; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D010316:Particle Size; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis; D011137:Polystyrenes / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004337:Drug Carriers; D011137:Polystyrenes