title: Characterization of a cDNA encoding a PSII-associated chlorophyll a/b-binding protein (CAB) from Chlamydomonas moewusii fitting into neither type I nor type II.
abstract: A full-length 1010-bp cDNA clone from Chlamydomonas moewusii coding for the precursor of a chlorophyll a/b-binding protein (CAB) was characterized. Northern analysis shows hybridization to a single 1150-base light-stimulated mRNA. Complementary hybrid-selected mRNAs were translated in vitro; SDS-PAGE indicates the synthesis of three polypeptides of 25, 27 and 28 kDa. Comparison of the deduced polypeptide sequence with other published CABs reveals greater similarity with PSII-associated proteins but, as with other algal CABs, our sequence does not meet established criteria for inclusion into either type I or type II, so branching of CABs into two types seems to have occurred after the divergence between algae and land plants.
authors.affiliation: Département de Biochimie, Faculté des Sciences et de Génie, Université Laval, Québec, Canada.
authors.forename: L
authors.initials: L
authors.lastname: Larouche
authors.forename: C
authors.initials: C
authors.lastname: Tremblay
authors.forename: C
authors.initials: C
authors.lastname: Simard
authors.forename: G
authors.initials: G
authors.lastname: Bellemare
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002696:Chlamydomonas / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000235:genetics*; D005796:Genes; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D005810:Multigene Family; D045322:Photosynthetic Reaction Center Complex Proteins / Q000145:classification / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D010940:Plant Proteins; D004247:DNA