title: Upstream thrombus growth impairs downstream thrombogenesis in non-anticoagulated blood: effect of procoagulant artery subendothelium and non-procoagulant collagen.
abstract: In the present experiments we have investigated the influence of wall shear rate and axial position on platelet and fibrin deposition which results when flowing human non-anticoagulated blood is exposed to either non-procoagulant fibrillar collagen (human type III) or procoagulant subendothelium (rabbit aorta). Platelet adhesion, thrombus volume and fibrin deposition were morphometrically evaluated at axial positions of 1 and 13 mm following perfusions for 5 min at shear rates of 100, 650 and 2,600 s-1. An axially-dependent decrease of platelet adhesion (34-57%, p less than 0.01-0.05) and thrombus volume (57-80%, p less than 0.05) was observed on collagen at all shear rates. On subendothelium, an axially-dependent decrease was observed for platelet adhesion only at 100 s-1 (29%; p less than 0.01) and for thrombus volume at shear rates of 650 s-1 and above (49-58%, p less than 0.01). Deposition of fibrin on subendothelium was axially decreased (16-42%, p less than 0.05) at all shear rates, while no significant axial differences were seen on collagen. However, substantially more fibrin was deposited on the subendothelium (p less than 0.05), and the upstream platelet adhesion and thrombus volume were lower than on collagen (p less than 0.05) at 100 s-1 and 650 s-1. The axially-dependent phenomena on the two surfaces are consistent with the concept of rapid-growing upstream thrombi which deplete the blood layer streaming adjacent ot the surface of platelets, leading to decreased platelet deposition further downstream.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Pharmaceutical Research Department, F. Hoffmann-La Roche Ltd, Basel, Switzerland.
authors.forename: K S
authors.initials: KS
authors.lastname: Sakariassen
authors.forename: H J
authors.initials: HJ
authors.lastname: Weiss
authors.forename: H R
authors.initials: HR
authors.lastname: Baumgartner
mesh_terms: D001777:Blood Coagulation / Q000502:physiology*; D003094:Collagen / Q000097:blood / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000502:physiology*; D006801:Humans; D010477:Perfusion; D010969:Plastics / Q000737:chemistry; D013927:Thrombosis / Q000097:blood*
chemical_list: D010969:Plastics; D003094:Collagen