[Parameters of lens protein fluorescence quenching in early stages of hereditary cataract].
Several physico-chemical parameters of lenses were investigated in IOR/Hab mice with hereditary cataract aged 4, 6, 8 weeks, i.e. at the stage preceding the development of cataract. The level of restored glutathione in the IOR/Hab lenses /2.0 mM/ was the lowest as compared to other lines but almost the same as in CBA/J /2.3 mM/. In the process of studying protein fluorescence quenching in cortex homogenates by nitrate anions a significant increase of the quenching constant was discovered in IOR/Hab mice: 10.4 M-1 in four-week animals and up to 32.3 M-1 in eight-week animals. The data obtained suggest that a progressing growth of the quenching constant in line IOR/Hab lenses is related to a decreased density of the protein negative charge resulted from their decreased phosphorylation.
Aĭtmagambetov
M T
MT
Deev
A I
AI
Kostenko
E R
ER
Vladimirov
Iu A
IuA
D000818:Animals; D002386:Cataract / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005136:Eye Proteins / Q000032:analysis*; D005453:Fluorescence; D005978:Glutathione / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008954:Models, Biological; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000032:analysis
D005136:Eye Proteins; D013438:Sulfhydryl Compounds; D005978:Glutathione
Parametry tusheniia fliuorestsentsii belkov khrustalika na rannikh stadiiakh nasledstvennoĭ katarakty.