[The effect of Yersinia pestis EV76 6 MD plasmid on the composition of outer membrane proteins of Yersinia pseudotuberculosis YPIII].
On the basis of Yersinia pseudotuberculosis strain YPIII the isogenic variants containing the different combinations of 47 Md plasmids from Yersinia pestis or Yersinia pseudotuberculosis cells with the 6 Md pYP plasmid from Yersinia pestis EV (intact or having impaired the pla gene determining the synthesis of plasmocoagulase). The degradation of the secreted proteins encoded by the 47 Md plasmids of Yersinia pestis and Yersinia pseudotuberculosis in the cells harbouring the 6Md pYP plasmid has been registered. Yersinia pseudotuberculosis strain YPIII carrying its own 47Md and pYP plasmids also contained no YOP1 protein, in contract to the parent strain. The damage of the pla gene eliminated the destructive effect on the outer membrane proteins. Imposition of the 47Md and 6Md plasmids from Yersinia pestis in Yersinia pseudotuberculosis cells may be used for obtaining and study of the physiological role of low molecular mass proteins resulting from proteolysis of proteins encoded by the 47Md virulence plasmid of Yersinia.
Zarenko
M I
MI
Goncharov
E K
EK
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D010957:Plasmids; D014774:Virulence; D015010:Yersinia pestis / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D015011:Yersinia pseudotuberculosis / Q000378:metabolism*
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; C064832:YadA protein, Yersinia
Vliianie 6 MD plazmidy Yersinia pestis EV76 na sostav vneshnemembrannykh belkov Yersinia pseudotuberculosis YPIII.