Image processing software for enhanced visualization of faint or noisy autoradiographic images.
A computer program for digital image processing is described which can be implemented using scanning densitometer hardware pre-existing in most biology departments plus computer video hardware which may either pre-exist in the biology department or would represent a moderate upgrade over an already planned computer purchase. The primary purpose of this computer program is to provide contrast enhancement of faint or low contrast autoradiograph images and to implement background subtraction and digital smoothing methods which permit visualization of blurry electrophoresis bands against noisy backgrounds. However, the program also has modest editing capabilities that allow its use in the routine preparation of images for publication. Finally, the program has facilities for deblurring, edge enhancement and multiple image averaging, which give it usefulness in other forms of photographic analysis.
Yee
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Molecular and Cell Biology Program, University of Texas, Dallas, Richardson 75083.
D001345:Autoradiography / Q000379:methods*; D001435:Bacteriophages / Q000648:ultrastructure; D004586:Electrophoresis; D009696:Nucleic Acids / Q000032:analysis; D011856:Radiographic Image Enhancement / Q000379:methods*; D012984:Software
D009696:Nucleic Acids