title: Host passage-dependent wheat germ agglutinin-binding proteins of Chlamydia trachomatis.
abstract: Wheat germ agglutinin (WGA)-binding proteins in the highly purified elementary bodies (EB) of Chlamydia trachomatis were detected by an ELISA-like assay of immobilized EB. Trypsin-sensitivity of the WGA-binding moieties was detected only in the chlamydiae grown in HeLa cells. A nonionic detergent, beta-octyl-D-glucopyranoside (OG), was used to extract proteins from the purified EB. Proteins in the extract were resolved by SDS-PAGE and probed with 125I-labelled WGA. Several proteins reacted with WGA in a specific manner. Some of these WGA-binding proteins in the EB of serovars L1 and E exhibited altered molecular mass after adaptation in the two alternate hosts, HeLa and McCoy cells. These results suggest that WGA-binding proteins exist in chlamydiae.
authors.affiliation: Department of Microbiology, St. Louis University School of Medicine, MO 63104-1028.
authors.forename: P C
authors.initials: PC
authors.lastname: Goswami
authors.forename: E
authors.initials: E
authors.lastname: Vretou
authors.forename: S K
authors.initials: SK
authors.lastname: Bose
mesh_terms: D002352:Carrier Proteins / Q000032:analysis*; D002692:Chlamydia trachomatis / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D014909:Wheat Germ Agglutinins / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D014909:Wheat Germ Agglutinins