Relationship of VLDL receptor and LPL in metabolism of VLDL by macrophage.
Macrophages were incubated with 125I-VLDL for 5 h in presence or absence of lipoprotein lipase (LPL) inhibitor, benzene boronic acid (BBA). Both the uptake and degradation of 125I-VLDL by macrophages were saturable, and the uptake and degradation curves were virtually identical. When macrophages were incubated with 125I-VLDL for 10 h in presence of BBA, the uptake and degradation of 125I-VLDL were still saturable. However, in absence of BBA, the uptake and degradation were no longer saturable. The results suggest that with macrophages incubated with VLDL for a shorter period, VLDL was taken up predominantly via receptor pathway, with a longer period of incubation, LPL played a striking role in uptake of VLDL.
Deng
Y Z
YZ
Department of Biochemistry, Tongji Medical University, Wuhan.
Feng
Z C
ZC
Wang
H X
HX
Jiang
W G
WG
Zhong
Y Q
YQ
Wang
C F
CF
D000818:Animals; D001897:Boronic Acids / Q000494:pharmacology; D002478:Cells, Cultured; D005260:Female; D008071:Lipoprotein Lipase / Q000502:physiology*; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D011973:Receptors, LDL / Q000502:physiology*; D013552:Swine
D001897:Boronic Acids; D008079:Lipoproteins, VLDL; D011973:Receptors, LDL; C036053:VLDL receptor; D008071:Lipoprotein Lipase; C010686:benzeneboronic acid