[The dynamics of postmortem cellular changes. Ultrastructural-histochemical research].
Lactate dehydrogenase (LDG), glucose-6-phosphate dehydrogenase (G-6-PDG), isocitrate dehydrogenase (ICDG) as well as acid phosphatase (AcPase) activities were assessed in the rat tracheal ciliated epithelial cells using cytophotometry, combined with ultrastructural AcPase demonstration within 1 to 5 hours after animal death. A moderate gradual reduction of LDG, G-6-PDG and AcPase, but not ICDG activities has been detected in the groups of initially unaffected and hypertensive rats. The activities of all dehydrogenases progressively decreased compared to the stable AcPase values in rats which died from acute renal failure. AcPase reaction products were found to be released from the Golgi apparatus and lysosomes into the adjacent cytoplasm and across the plasma membrane. Occasionally AcPase activity emerged in the cis- and intermediate lamellae of the Golgi apparatus. The perturbations in the membrane permeability evidenced by AcPase leakage can be considered as the most likely mechanism for the observed postmortem reduction of some enzymatic activities tested.
Sipovskiĭ
V G
VG
Valeev
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RI
D000135:Acid Phosphatase / Q000378:metabolism; D058186:Acute Kidney Injury / Q000201:enzymology / Q000473:pathology; D000818:Animals; D002923:Cilia / Q000201:enzymology / Q000648:ultrastructure; D004848:Epithelium / Q000201:enzymology / Q000648:ultrastructure; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006651:Histocytochemistry; D006977:Hypertension, Renal / Q000201:enzymology / Q000473:pathology; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D011180:Postmortem Changes; D051381:Rats; D013997:Time Factors; D014132:Trachea / Q000201:enzymology / Q000648:ultrastructure
D007770:L-Lactate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D000135:Acid Phosphatase
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