Potential artifact for the increase of tumor associated antigens in serum samples from patients injected with monoclonal antibodies.
The administration of [131I]B72.3 MAb for both diagnostic and therapeutic purposes is followed by the development of human anti-mouse antibodies (HAMA) in approx. 70% of patients receiving i.v. doses of MAb above 1 mg. The presence of HAMA in circulation interferes in the detection of the tumor-associated glycoprotein TAG-72 using the CA 72-4 RIA. We have reported elsewhere (Ferroni et al., 1990) that heat treatment at 90 degrees C, pH 6.5, for 15 min is capable of precipitating and/or inactivating HAMA without causing any significant loss in antigen recovery. Furthermore, the removal of the interfering activity leads to a more correct evaluation of TAG-72 serum levels in HAMA-positive serum samples and to a continued use of TAG-72 in the follow-up of patients undergoing MAb-based clinical protocols.
Ferroni
P
P
Laboratory of Tumor Immunology and Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Milenic
D E
DE
Roselli
M
M
Carrasquillo
J A
JA
Raubitschek
A
A
Schlom
J
J
Colcher
D
D
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis / Q000097:blood*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000097:blood*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000097:blood; D003110:Colonic Neoplasms / Q000097:blood; D005189:False Positive Reactions; D005260:Female; D006023:Glycoproteins / Q000097:blood; D006358:Hot Temperature; D006801:Humans; D051379:Mice; D010051:Ovarian Neoplasms / Q000097:blood; D011863:Radioimmunoassay
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D006023:Glycoproteins; C047052:tumor-associated antigen 72