Intracellular Ca2+ and Mg2+ regulation for insulin-stimulated glucose uptake into mouse diaphragm muscles.
We investigated whether the changed levels of intracellular Ca2+ and Mg2+ in mouse skeletal muscles affected insulin-stimulated glucose uptake. Insulin alone had no effect on 45Ca2+ efflux and uptake. A23187 at 20 microM increased 45Ca2+ influx and inhibited insulin-stimulated 14C-glucose uptake with normal external concentrations of Ca2+ and Mg2+ or in the absence of both cations. These results suggest that the increase in Ca2+ influx into, and in Mg2+ efflux from, skeletal muscles may inhibit insulin-stimulated glucose uptake.
Naitoh
T
T
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Kobayashi
S
S
Kimura
I
I
Kimura
M
M
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology*; D003964:Diaphragm / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D008274:Magnesium / Q000502:physiology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009132:Muscles / Q000378:metabolism*
D007328:Insulin; D000001:Calcimycin; D008274:Magnesium; D005947:Glucose; D002118:Calcium