The Adh in Drosophila: chromosomal location and restriction analysis in species with different phylogenetic relationships.
Restriction analysis of the genomic region containing the Adh gene and in situ hybridization assays were performed in six Drosophila species belonging to three different subgenera: D. ambigua, D. subobscura, D. madeirensis and D. guanche (sg. Sophophora); D. immigrans (sg. Drosophila); and D. lebanonensis (sg. Pholadoris). In agreement with previous observations, comparison of restriction maps of the Adh region shows that D. subobscura and D. madeirensis are very closely related. Partial homology is also observed with the rest of the obscura group species. Nevertheless, no resemblance at the restriction map level is detected when more distantly related species are compared. In D. ambigua, D. immigrans and D. lebanonensis in situ hybridization assays reveal a single chromosomal location for Adh, which in D. lebanonensis appears to be sex linked. In contrast, in D. subobscura, D. madeirensis and D. guanche multiple sites of hybridization with homologous and heterologous probes are observed. For example, in D. subobscura and D. madeirensis the functional Adh gene is located on the U chromosome and additional homologous retrosequences are found on the E chromosome.
Visa
N
N
Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Spain.
Marfany
G
G
Vilageliu
L
L
Albalat
R
R
Atrian
S
S
Gonzàlez-Duarte
R
R
D000426:Alcohol Dehydrogenase / Q000145:classification / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA; D004330:Drosophila / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D015183:Restriction Mapping; D012469:Salivary Glands / Q000166:cytology; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D004247:DNA; D000426:Alcohol Dehydrogenase