title: A simple method for cloning Giardia duodenalis from cultures and fecal samples.
abstract: Using a novel method for cloning Giardia duodenalis from cultures and fecal samples, 47 clones from 7 isolates were established in vitro. Average colony-forming efficiency in established cultures was 43.2% compared to 11.2% when cloning directly from excystation. The highest success rate of cloning was found with the Portland (P1, ATCC No. 30888) isolate, with a colony-forming efficiency of 92.7%. Cloned and parent populations were compared over a range of 13 enzymes using starch gel electrophoresis. No genetic difference was found between any of the clones and the parent isolates.
authors.affiliation: Division of Veterinary Biology, School of Veterinary Studies, Murdoch University, Western Australia.
authors.forename: N
authors.initials: N
authors.lastname: Binz
authors.forename: R C
authors.initials: RC
authors.lastname: Thompson
authors.forename: B P
authors.initials: BP
authors.lastname: Meloni
authors.forename: A J
authors.initials: AJ
authors.lastname: Lymbery
mesh_terms: D000818:Animals; D005243:Feces / Q000469:parasitology*; D005872:Giardia / Q000254:growth & development*; D008722:Methods