Method for the concentration of infectious pancreatic necrosis virus from hatchery water.
A method was developed for concentrating infectious pancreatic necrosis virus from hatchery water using positively charged 1-MDS filters. The method consists of passing large volumes (Ca. 1001) of hatchery water through 1-MDS microporous filter followed by the elution of the adsorbed virus using a high pH buffer. The virus adsorbed efficiently to 1-MDS filters when the pH of the water was 5.5 and was eluted optimally with 3% beef extract solution (pH 10). This procedure permitted the processing of 100 1 of hatchery water which resulted in a 300-fold reduction in the volume of water and greater than 90% recovery of the seeded virus.
Maheshkumar
S
S
Department of Veterinary Diagnostic Investigation, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
Goyal
S M
SM
Peterson
R B
RB
Economon
P P
PP
D000327:Adsorption; D000535:Aluminum; D000077410:Aluminum Chloride; D017607:Aluminum Compounds; D000818:Animals; D002478:Cells, Cultured; D002712:Chlorides; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D012087:Reoviridae / Q000302:isolation & purification*; D014337:Trout / Q000382:microbiology*; D014871:Water Microbiology
D017607:Aluminum Compounds; D002712:Chlorides; D003470:Culture Media; D000077410:Aluminum Chloride; D000535:Aluminum