title: Evidence for motility and pinocytosis in ramified microglia in tissue culture.
abstract: Ramified microglial cells were investigated in primary cultures of dissociated cerebral cortical tissue from rats. The identification of these cells was confirmed through immunohistochemical staining with 7 monoclonal antibodies selective for microglia. While there was significant variation in staining intensity with different antibodies, all stained the identified ramified cells; the antibodies OX-42 and ED1 yielded the most intense immunoreactivity. Based on distinctive morphological features, the microglia could be identified in living cultures where they were monitored using time-lapse video recording. This technique revealed extremely dynamic features of cellular plasticity and motility. Ramified microglia exhibited constant and rapid alterations in the size and shape of their cell body with an associated extension and retraction of processes; concomitantly, the cells moved about in a circumscribed area. These features of plasticity and motility were unique to this cell type, and correlated with OX-42 immunostaining. The microglia also possessed a differentially high level of pinocytotic activity; this too was correlated with OX-42 staining. From the nature of their morphological plasticity and motility, high pinocytosis, and cellular distribution, it is hypothesized that the ramified microglia specifically function as a system of fluid cleansing in normal brain tissue.
authors.affiliation: Department of Oral Biology, College of Dentistry, Ohio State University, Columbus 43210.
authors.forename: P L
authors.initials: PL
authors.lastname: Booth
authors.forename: W E
authors.initials: WE
authors.lastname: Thomas
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002465:Cell Movement; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000502:physiology*; D005333:Fetus; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D007546:Isoquinolines; D008648:Mesoderm / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D010873:Pinocytosis; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D007546:Isoquinolines; C017475:lucifer yellow