Structural characterization of heme sites in spinach cytochrome b6f complexes: a resonance Raman study.
Resonance Raman spectra of cytochrome b6f complexes isolated from spinach chloroplasts have been obtained. Selective resonance enhancements and partial reductions of the complex by redox mediators were used to isolate and identify the contributions of heme b6 and heme f sites to the observed spectra. Corresponding spectra for turnip cytochrome f have also been obtained. Power-dependent photoreduction was observed in cytochrome f of the complex as well as in the isolated cytochrome f during the course of the Raman experiments.
Hobbs
J D
JD
Department of Chemistry, University of New Mexico, Albuquerque 87131.
Wynn
M
M
Nunez
D J
DJ
Malkin
R
R
Knaff
D B
DB
Ondrias
M R
MR
D003580:Cytochromes / Q000737:chemistry*; D045348:Cytochromes f; D006418:Heme / Q000737:chemistry*; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
D003580:Cytochromes; D010940:Plant Proteins; D006418:Heme; D045348:Cytochromes f