Serological discrimination between HTLV-I and HTLV-II antibodies by ELISA using synthetic peptides as antigens.
Using the peptides from amino acids 100-130 of the HTLV-I gag protein, 175-199 of the HTLV-I env protein and the corresponding peptides of HTLV-II (amino acids 106 to 135 of the gag protein and 171 to 196 of the env protein), we tested for reactivity against antibodies by enzyme immunoassay in sera from HTLV-I and HTLV-II carriers. The peptides derived from the env proteins have high specificity for antibody binding. The peptide based on amino acids 175-199 of HTLV-I reacted with antibodies in sera from all HTLV-I carriers, and the peptide composed of amino acids 171-196 of HTLV-II reacted with antibodies in sera from all HTLV-II carriers. For the peptides derived from the gag proteins, we observed some cross-reactivity in sera from persons with anti-HTLV-I and anti-HTLV-II, due to antibody binding to the peptide corresponding to 12 amino acids from the C-terminal end of the gag protein. Separate enzyme immunoassays that used the four synthetic peptides as antigens clearly distinguished between serum with antibodies to HTLV-I or HTLV-II in various individuals and excluded false positive results using the particle agglutination assay that used a whole-virus lysate of HTLV-I as antigen.
Washitani
Y
Y
Fukuoka Red Cross Blood Center, Japan.
Kuroda
N
N
Shiraki
H
H
Nishimura
Y
Y
Yamaguchi
K
K
Takatsuki
K
K
Fernando
L P
LP
Fang
C T
CT
Kiyokawa
H
H
Maeda
Y
Y
D000595:Amino Acid Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D015686:Gene Products, env / Q000737:chemistry / Q000276:immunology; D015683:Gene Products, gag / Q000737:chemistry / Q000276:immunology; D015481:HTLV-I Antibodies / Q000032:analysis* / Q000737:chemistry; D015486:HTLV-I Antigens / Q000276:immunology; D015490:HTLV-I Infections / Q000175:diagnosis*; D015482:HTLV-II Antibodies / Q000032:analysis* / Q000737:chemistry; D015487:HTLV-II Antigens / Q000276:immunology; D015491:HTLV-II Infections / Q000175:diagnosis*; D006801:Humans; D008969:Molecular Sequence Data
D015686:Gene Products, env; D015683:Gene Products, gag; D015481:HTLV-I Antibodies; D015486:HTLV-I Antigens; D015482:HTLV-II Antibodies; D015487:HTLV-II Antigens