title: [II. Use of antibodies to DNA modified by trans-diaminodichloroplatinum, for identification of specific DNA sequences].
abstract: DNA modified by trans diaminedichlorplatinum-II (transDDP) has been suggested as an effective probe for non-isotopic hybridization and high-specific anti-DNA-transDDP antibodies with horse radish peroxidase or alkaline phosphotase conjugated antibodies to rabbit Ig and protein A-peroxidase - for hybrids visualization. This method allows to detect 2 pg/mm DNA.
authors.forename: V I
authors.initials: VI
authors.lastname: Kiseleva
authors.forename: M F
authors.initials: MF
authors.lastname: Turchinskiĭ
authors.forename: A M
authors.initials: AM
authors.lastname: Poverennyĭ
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001483:Base Sequence; D002417:Cattle; D002945:Cisplatin / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects / Q000276:immunology*; D007124:Immunoenzyme Techniques; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D013237:Stereoisomerism; D013950:Thymus Gland / Q000378:metabolism
chemical_list: D000906:Antibodies; C034697:transplatin; D004247:DNA; D002945:Cisplatin
vernacular_title: II. Ispol'zovanie antitel k DNK, modifitsirovannoĭ trans-diamindikhlorplatinoĭ, dlia identifikatsii spetsificheskikh posledovatel'nosti DNK.