Rapid screening method for the determination of diethylstilbestrol in edible animal tissue by column liquid chromatography with electrochemical detection.
A rapid and sensitive screening method for the determination of residues of diethylstilbestrol in edible animal tissue is described. The analyte was extracted from the tissue with tert.-butyl methyl ether, reextracted with 1 M sodium hydroxide and further cleaned up by solid-phase extraction with C18 cartridges. Analysis was performed by isocratic elution with a phosphate-buffered mobile phase, methanol-0.05 M phosphate buffer pH 3.5 (67:33), on a Nucleosil 5-microns C18 column with electrochemical detection at +0.90 V. The average recovery of trans-diethylstilbestrol in spiked samples is 66%, with a standard deviation of 14% (n = 22) in the range 0.5-2.0 microgram/kg. The detection limit is 0.1-0.2 microgram/kg, although at this level other compounds may interfere and give rise to false positive results.
Reuvers
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Servicio de Bromatología, Centro nacional de Alimentación, majadahonda, Spain.
Perogordo
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Jiménez
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D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004054:Diethylstilbestrol / Q000032:analysis*; D004563:Electrochemistry; D008460:Meat / Q000032:analysis*
D004054:Diethylstilbestrol