title: Rapid antimicrobial susceptibility testing of gram-negative bacilli using Baxter MicroScan rapid fluorogenic panels and autoSCAN-W/A.
abstract: The MicroScan Rapid Neg MIC/Combo panels and autoSCAN-W/A (Walk Away) system utilize automated fluorescence technology for rapid antimicrobial susceptibility testing of Gram-negative bacilli. In a three site clinical study eleven antimicrobial agents were evaluated by comparing results obtained with 741 clinical isolates, using rapid fluorogenic expanded dilution MIC panels and corresponding frozen microdilution reference panels determined visually. Results for 31%, 40%, 12% and 9% of the isolates were available within 3.5, 4.5, 5.5 and 7.0 hours respectively. Results for 7.3% were not available within that time period. For the seven drugs analyzed using a Minimum Inhibitory Concentration range of dilutions, overall agreement (+/- 1 dilution) was 94%, with 1.5% very major, 0.9% major and 2.5% minor errors. For the four drugs analyzed using a Breakpoint range of dilutions, overall agreement (+/- 1 dilution) was 97%, with two percent very major, and one percent major errors. The MicroScan Rapid Neg MIC system is an accurate and rapid method for same day determination of susceptibility of Gram-negative bacilli.
authors.affiliation: Baxter Healthcare corporation, MicroScan Division, Statistical Analysis Group, West Sacramento, California 95691.
authors.forename: J H
authors.initials: JH
authors.lastname: Godsey
authors.forename: S
authors.initials: S
authors.lastname: Bascomb
authors.forename: T
authors.initials: T
authors.lastname: Bonnette
authors.forename: M
authors.initials: M
authors.lastname: Kangas
authors.forename: K
authors.initials: K
authors.lastname: Link
authors.forename: K
authors.initials: K
authors.lastname: Richards
authors.forename: K M
authors.initials: KM
authors.lastname: Tomfohrde
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D066298:In Vitro Techniques; D007769:Lactams; D018942:Macrolides; D008826:Microbial Sensitivity Tests / Q000295:instrumentation* / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D007769:Lactams; D018942:Macrolides