Trace analysis of N-nitrosoureas by their alkylating activity.
N-Nitrosoureas can be analysed indirectly by gas chromatography with chemoluminescence detection after reaction of the corresponding diazoalkanes with the scavenger reagent N-nitroso-N-tert-butylglycine. A lower determination limit of 2 ng N-methyl-N-nitrosourea per sample is achieved. The method is specific for N-nitroso compounds, which release nonpolar diazoalkanes upon alkaline treatment.
Mende
P
P
German Cancer Research Centre, Institute of Toxicology and Chemotherapy, Heidelberg.
Spiegelhalder
B
B
Preussmann
R
R
D000478:Alkylation; D002849:Chromatography, Gas; D009607:Nitrosourea Compounds / Q000032:analysis*
D009607:Nitrosourea Compounds