title: The prevention of adsorption of interferents to radiolabelled protein by Tween 20.
abstract: Radiolabels are often used to quantitatively determine the amount of protein immobilized on chromatographic supports, immunochemical plates and biosensor surfaces. Bovine serum albumin (BSA) was chosen as a model protein for quantitative deposition studies. BSA was radioiodinated (125I-) or fluorescently labelled (fluorescein), then incubated with the following surfaces: quartz, quartz derivatized by 3-aminopropyltriethoxysilane (Qz-APTES), and Qz-APTES reacted with glutaraldehyde or tresyl chloride. The amounts of BSA immobilized to the different surfaces were compared using data from radioactivity and fluorescence assays. Irreproducible results were obtained with radioiodinated BSA due to adsorption/desorption behaviour of an unidentified radioactive species. When the non-ionic detergent Tween 20 was added to the protein/surface incubation mixture, radiolabelled BSA gave reproducible protein binding results which agreed with fluorescent protein binding patterns. The effect of Tween 20 was due to either the binding to BSA displacing the interferent and/or the solubilization of the interferent.
authors.affiliation: Department of Chemistry, University of Toronto, Mississauga, Ontario, Canada.
authors.forename: E T
authors.initials: ET
authors.lastname: Vandenberg
authors.forename: U J
authors.initials: UJ
authors.lastname: Krull
mesh_terms: D000327:Adsorption; D001665:Binding Sites; D005453:Fluorescence; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D011136:Polysorbates; D011485:Protein Binding; D011791:Quartz; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission
chemical_list: D007457:Iodine Radioisotopes; D011136:Polysorbates; D011791:Quartz; D012710:Serum Albumin, Bovine