title: Antioxidative effect of protoporphyrin on lipid peroxidation in tissue homogenates of intravenously administered rats.
abstract: Effect of intravenous administration of protoporphyrin IX (PP) on lipid peroxidation was studied in rats. PP and/or PP-derived porphyrins were found to be mainly distributed in livers, spleen and lungs. Dose-dependent decreases in the Fe2+ and L-ascorbic acid-stimulated lipid peroxidation in homogenates of livers and dose-dependent increases in porphyrin concentration in livers were observed after the PP injection. In the experiments with a 20 mg/kg dose of PP, the peroxidation level in the liver homogenates reached its minimum level during the period of 3 to 24 h accompanying the high porphyrin concentration in livers after the administration. After 96 h, a relatively high porphyrin concentration was still retained, but decreases in the peroxidation levels had ceased. PP administration caused a dose-dependent decrease in the endogenous lipid peroxides in livers within 0.5 h and the low levels were maintained throughout the course of the 168-h study. These results clearly show that the administered PP is distributed in the liver and inhibits the lipid peroxidation in vivo.
authors.affiliation: Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Imai
authors.forename: T
authors.initials: T
authors.lastname: Aimoto
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: R
authors.initials: R
authors.lastname: Kimura
mesh_terms: D000818:Animals; D000975:Antioxidants; D066298:In Vitro Techniques; D007275:Injections, Intravenous; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D009928:Organ Specificity / Q000502:physiology; D011524:Protoporphyrins / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000975:Antioxidants; D011524:Protoporphyrins