Detection of Clostridium perfringens type A enterotoxin in faecal and food samples using immunomagnetic separation (IMS)-ELISA.
A simple, rapid and sensitive immunoassay, based on immunomagnetic particles (Dynabeads M-280) was developed for detection and quantitation of Clostridium perfringens type A enterotoxin from faecal and food extracts. The assay had a detection limit of 2.5 ng/ml enterotoxin in homogenates of faeces and inoculated meat extracts. The specificity was confirmed by both crossed immunoelectrophoresis and Western immunoblotting techniques, using a purified enterotoxin as standard.
Cudjoe
K S
KS
MATFORSK, Norwegian Food Research Institute, As.
Thorsen
L I
LI
Sørensen
T
T
Reseland
J
J
Olsvik
O
O
Granum
P E
PE
D000818:Animals; D015153:Blotting, Western; D003016:Clostridium perfringens; D004768:Enterotoxins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005243:Feces / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods*; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008863:Microspheres; D015203:Reproducibility of Results
D004768:Enterotoxins; C035598:enterotoxin, Clostridium