title: Proto-oncogenes of genomic DNA in clinically normal animals of various species.
abstract: To provide information about oncogenes for molecular biological studies of tumors in domestic animals, the proto-oncogenes homologous to the c-myc, c-erbB-2, c-ros-1, c-yes-1, v-myc, v-Ki-ras, and v-Ha-ras oncogenes of genomic DNA in cattle, horses, pigs, dogs, cats, and chickens were investigated by Southern blot hybridization. High molecular weight genomic DNA in each of the animals contained proto-oncogenes that had a certain homology with the oncogenes used, but the extent of nucleotide homology of the proto-oncogenes differed in number and molecular weight: ie, 1 or 2 bands at 1.6 to 22.0 kilobase (kb) in the c-myc probe, 1 or 2 bands at 1.1 to 16.0 kb in the c-ros-1 probe, 1 to 3 bands at 0.7 to 23.0 kb in the c-erbB-2 probe, 1 to 4 bands at 0.6 to 18.0 kb in the c-yes-1 probe, 1 to 3 bands at 1.6 to 30.0 kb in the v-myc probe, 1 to 7 bands at 1.0 to 36.0 kb in the v-Ki-ras probe, and 1 to 4 bands at 1.0 to 27.0 kb in the v-Ha-ras probe. Furthermore, signal strength of each band, as determined by autoradiography, was not always the same for each probe in the various animals. Our findings indicate that these proto-oncogenes are well conserved with species specificities in each animal.
authors.affiliation: Department of Veterinary Pathology, Faculty of Agriculture, Miyazaki University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Miyoshi
authors.forename: S
authors.initials: S
authors.lastname: Tateyama
authors.forename: K
authors.initials: K
authors.lastname: Ogawa
authors.forename: D
authors.initials: D
authors.lastname: Nosaka
authors.forename: T
authors.initials: T
authors.lastname: Ohashi
authors.forename: B
authors.initials: B
authors.lastname: Sunyasootcharee
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002415:Cats / Q000235:genetics; D002417:Cattle / Q000235:genetics; D002645:Chickens / Q000235:genetics*; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D004285:Dogs / Q000235:genetics; D006736:Horses / Q000235:genetics; D008297:Male; D008322:Mammals / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011519:Proto-Oncogenes; D013552:Swine / Q000235:genetics; D013737:Testis / Q000737:chemistry
chemical_list: D015342:DNA Probes; D004273:DNA, Neoplasm