Quantitative analysis of electron energy-loss spectra from ultrathin-sectioned biological material. I. Optimization of the background-fit with the use of Bio-standards.
A computer program for quantitative spectral analysis is proposed for the elemental analysis of biological material by electron energy-loss spectroscopy in a conventional transmission electron microscope, the Zeiss EM902. Bio-standards are used to test the performance of this program. The application of a simplex optimization method for curve-fitting is proposed to separate the ionization edge from the background. Making use of Ce-, Ca- and Fe-bio-standards, this method is compared with Egerton's well-known two-area method.
Sorber
C W
CW
AEM Unit, Clinical Pathological Institute I, Rotterdam, The Netherlands.
Ketelaars
G A
GA
Gelsema
E S
ES
Jongkind
J F
JF
De Bruijn
W C
WC
D002118:Calcium / Q000737:chemistry; D002563:Cerium / Q000737:chemistry; D007091:Image Processing, Computer-Assisted; D007501:Iron / Q000737:chemistry; D008854:Microscopy, Electron / Q000379:methods*; D008867:Microtomy; D013057:Spectrum Analysis / Q000379:methods*
D002563:Cerium; D007501:Iron; D002118:Calcium