Two genes encoding putative mitochondrial alcohol dehydrogenases are present in the yeast Kluyveromyces lactis.
Four structural genes encoding isozymes of the alcohol dehydrogenase (ADH) system in the yeast Kluyveromyces lactis have been identified by hybridization to ADH2 DNA probes from Saccharomyces cerevisiae. In this paper we report on the isolation of KlADH4 and the complete sequencing of KlADH3 and KlADH4, two genes which show high homology to KlADH1, the ADH gene previously isolated in K. lactis, and to the ADH genes of S. cerevisiae. When compared with KlADH1, both KlADH3 and KlADH4 encode amino-terminal extensions which show the characteristics of the mitochondrial targeting sequences. These extensions are poorly conserved both at the nucleotide and the amino acid level. Surprisingly, the KlADH4 extension shows a higher identity at the amino acid level to the one encoded by ADH3 of S. cerevisiae than to the KlADH3 presequence. KlADH3 and KlADH4, in contrast to the ADH3 gene of S. cerevisiae, show a strong bias in the choice of codons.
Saliola
M
M
Department of Cell and Developmental Biology, University of Rome, La Sapienza Città Universitaria, Italy.
Gonnella
R
R
Mazzoni
C
C
Falcone
C
C
D000426:Alcohol Dehydrogenase / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes; D004271:DNA, Fungal / Q000032:analysis / Q000737:chemistry*; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005822:Genetic Vectors; D007527:Isoenzymes / Q000235:genetics*; D007716:Kluyveromyces / Q000201:enzymology / Q000235:genetics*; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D004271:DNA, Fungal; D007527:Isoenzymes; D000426:Alcohol Dehydrogenase