Evidence for the activity of a phospholipid exchange protein in vivo.
Liposomes containing a self quenching concentration of a fluorescent phosphatidylethanolamine analog, were microinjected into Chinese hamster ovary cells. Immediately after microinjection, little intracellular fluorescence was observed. 10 min post-injection, labeling of the nuclear envelope and mitochondria became evident. Combined with control studies, our results suggest that phospholipid exchange protein(s) facilitates phosphatidylethanolamine movement in vivo.
Sleight
R G
RG
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, OH 45267-0522.
Hopper
K
K
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D008081:Liposomes; D008565:Membrane Proteins / Q000378:metabolism*; D008845:Microinjections; D010714:Phosphatidylethanolamines / Q000737:chemistry; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids / Q000378:metabolism*
D002352:Carrier Proteins; D005456:Fluorescent Dyes; D008081:Liposomes; D008565:Membrane Proteins; D010714:Phosphatidylethanolamines; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids