title: Isolation and characterization of cDNAs encoding beta A2- and beta A4-crystallins: heterologous interactions in the predicted beta A4-beta B2 heterodimer.
abstract: Except for the two acidic chains, beta A2 and beta A4, the primary structures of all bovine beta-crystallins have previously been elucidated, either by direct protein sequencing or prediction from cDNA sequencing. Both beta A2 and beta A4 were found to be synthesized in half-year-old calf lenses and are therefore likely to be present in a cDNA bovine library constructed from mRNA isolated from lenses of that age. A large number of cDNA clones was screened with all available crystallin, actin, vimentin and lens membrane protein MP26 probes and finally with a randomly primed mRNA probe. Clones positive for the latter, but negative for known lens proteins, were isolated and sequenced. beta A2, comprising 197 aa, and beta A4, comprising 209 aa, were identified. Both proteins have a conserved two-domain structure and an N-terminal extension which is variable. A three-dimensional model of the structure of beta A4 was made based on the coordinates of one subunit from the beta B2 dimer which has recently been solved using x-ray diffraction techniques. The resulting heterodimer structure, together with the compiled bovine beta-crystallin sequences, was used to indicate those regions of the sequences which distinguish acidic from basic beta-crystallins with a view to defining structural features necessary for subunit recognition in beta-crystallin aggregates. With the aid of the present data, the complete evolutionary tree of the bovine beta-crystallin family has been constructed, which confirms the early separation of the genes encoding the three acidic and the three basic beta-crystallins.
authors.affiliation: Department of Biochemistry, University of Nijmegen, The Netherlands.
authors.forename: G L
authors.initials: GL
authors.lastname: van Rens
authors.forename: H P
authors.initials: HP
authors.lastname: Driessen
authors.forename: V
authors.initials: V
authors.lastname: Nalini
authors.forename: C
authors.initials: C
authors.lastname: Slingsby
authors.forename: W W
authors.initials: WW
authors.lastname: de Jong
authors.forename: H
authors.initials: H
authors.lastname: Bloemendal
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D003001:Cloning, Molecular; D003459:Crystallins / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D008969:Molecular Sequence Data; D010802:Phylogeny; D011487:Protein Conformation; D016415:Sequence Alignment
chemical_list: D003459:Crystallins; D004247:DNA