Stimulation of pulmonary surfactant secretion by activating neutrophils in rat type II pneumocytes culture.
The influence of activating neutrophils on the secretion of phosphatidylcholine (PC), the predominant component of pulmonary surfactant, was examined using primary culture of rat type II pneumocytes. Simultaneous addition of neutrophils and opsonized zymosan, but not neutrophils or opsonized zymosan alone, to type II pneumocytes caused a significant increase in PC secretion without affecting the release of lactate dehydrogenase, a marker of cytotoxicity. The increase in PC secretion was dependent on the number of activating neutrophils. In addition, pretreatment of culture with the combination of superoxide dismutase and catalase inhibited the increase in PC secretion. These findings indicate that activating neutrophils stimulate the secretion of pulmonary surfactant and that the stimulation is mediated by oxygen radicals.
Oda
Y
Y
Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumato University, Japan.
Kai
H
H
Isohama
Y
Y
Takahama
K
K
Miyata
T
T
D000818:Animals; D002374:Catalase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D008297:Male; D009504:Neutrophils / Q000187:drug effects*; D010713:Phosphatidylcholines / Q000378:metabolism; D011663:Pulmonary Surfactants / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000378:metabolism; D015054:Zymosan / Q000494:pharmacology
D010713:Phosphatidylcholines; D011663:Pulmonary Surfactants; D015054:Zymosan; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase