In vitro cytotoxicity studies with the fish hepatoma cell line, PLHC-1 (Poeciliopsis lucida).
The PLHC-1 fish hepatoma cell line (Poeciliopsis lucida) was used in the neutral red assay to evaluate the acute cytotoxicities of direct-acting (alkylbenzenes, phthalate diesters, and pesticides) and metabolism-mediated (benzo[a]pyrene) toxicants. The sequence of cytotoxic potencies for the alkylbenzenes and phthalate diesters appeared to be a direct function of their hydrophobicity (as described by logarithmic octanol/water partition coefficients). The organochlorine pesticides (alachlor and p,p'-methoxychlor) were more cytotoxic than the organophosphorus pesticides (EPN, diazinon, and malathion). The PLHC-1 cell line apparently maintained sufficient xenobiotic-metabolizing capacity, as the hepatoma cells were able to metabolize benzo[a]pyrene to cytotoxic intermediates. Xenobiotic-metabolizing capacity was temperature dependent, with enzymatic activity increasing as the temperature was increased from 28 to 34 to 37 degrees C, was inducible by Aroclor 1254 (a chemical inducer of cytochrome P450-dependent monooxygenase activity), and was reduced by EPN (an inhibitor of P450 activity).
Babich
H
H
Laboratory Animal Research Center, Rockefeller University, New York, New York 10021.
Rosenberg
D W
DW
Borenfreund
E
E
D015660:7-Alkoxycoumarin O-Dealkylase / Q000378:metabolism*; D000818:Animals; D001555:Benzene Derivatives / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D008114:Liver Neoplasms, Experimental; D010575:Pesticides / Q000633:toxicity*; D010795:Phthalic Acids / Q000633:toxicity*; D011036:Poecilia
D001555:Benzene Derivatives; D010575:Pesticides; D010795:Phthalic Acids; D015660:7-Alkoxycoumarin O-Dealkylase