title: Estradiol-induced vitellogenin gene expression in a teleost fish, Oreochromis aureus.
abstract: Vitellogenesis presents a versatile model for the study of hormone-induced gene expression. We report here the effects of estradiol-17 beta-propionate on vitellogenin gene expression in male Oreochromis aureus, a teleost fish. Vitellogenin mRNA of 6500 nucleotides has been elucidated from the livers of female and estradiol-treated male O. aureus. By hybridization with a specific O. aureus cDNA probe, the vitellogenin mRNA transcript was detected as early as 1 hr following primary and secondary estradiol-stimulations, although for the latter, the rate of accumulation of vitellogenin-specific mRNA was 20-fold higher. The vitellogenin mRNA peaked at 72 and 48 hr, respectively, for primary and secondary stimulations. At the translational level, the increase in plasma vitellogenin was further enhanced during the secondary stimulation. There was a distinct shift in the peak of plasma vitellogenin from Day 14 in the primary induction to Day 3 in the secondary stimulation. The plasma vitellogenin presented in two forms, 300 and 500 kDa, both of which were immunologically confirmed by Western blot analysis to be vitellogenin proteins.
authors.affiliation: Department of Zoology, National University of Singapore.
authors.forename: E H
authors.initials: EH
authors.lastname: Lim
authors.forename: J L
authors.initials: JL
authors.lastname: Ding
authors.forename: T J
authors.initials: TJ
authors.lastname: Lam
mesh_terms: D000818:Animals; D015342:DNA Probes; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005399:Fishes / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects*; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008297:Male; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic; D014819:Vitellogenins / Q000097:blood / Q000235:genetics*
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger; D014819:Vitellogenins; D004958:Estradiol