Detection of the human immunodeficiency virus genome with a biotinylated DNA probe generated by polymerase chain reaction.
A non-radiolabelled DNA probe was developed for detection of the human immunodeficiency virus type 1 (HIV-1) genome using the polymerase chain reaction (PCR) technology. Primers amplifying a 395 base pair segment of a portion of the polymerase region of the HIV-1 genome were used both to amplify sample target DNA and to generate a biotinylated DNA probe used in Southern blot hybridization. This probe performed as well as one produced by nick translation using biotinylated nucleotides or an enzyme labelled oligonucleotide probe.
Forghani
B
B
Viral and Rickettsial Disease Laboratory, California State Department of Health Services, Berkeley 94704.
Hurst
J W
JW
Shell
G R
GR
D001426:Bacterial Proteins; D001483:Base Sequence; D001710:Biotin; D015152:Blotting, Northern; D015139:Blotting, Southern; D015342:DNA Probes; D015751:Genes, pol / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D019809:Streptavidin
D001426:Bacterial Proteins; D015342:DNA Probes; D001710:Biotin; D019809:Streptavidin