[Pharmacological studies on leaf of Arctostaphylos uva-ursi (L.) Spreng. III. Combined effect of arbutin and indomethacin on immuno-inflammation].
A study was carried out to examine the combined effect of arbutin isolated from the leaves of Arctostaphylos uva-ursi (L.) Spreng. (Bearberry leaf) and indomethacin on Type IV allergic reaction-induced immuno-inflammation, carrageenin-induced edema and adjuvant-induced arthritis. Contact dermatitis caused by picryl chloride (PC-CD) and sheep red cell delayed type hypersensitivity (SRBC-DTH) was not inhibited by the oral application of arbutin at 2 divided doses immediately before and 16 h after the application, but arbutin at dose of 50 mg/kg 24 h after the application rapidly decreased the swelling of PC-CD. Arbutin (p.o.) plus indomethacin (s.c.) showed inhibitory effect on the swelling of PC-CD and SRBC-DTH stronger than that of indomethacin alone. When arbutin (p.o.) and indomethacin (s.c.) in a given portion were simultaneously administered, the inhibitory effect was more potent than that of indomethacin alone in both carrageenin-induced edema and adjuvant-induced arthritis. These results suggest that arbutin may increase the inhibitory action of indomethacin on PC-CD, SRBC-DTH, carrageenin-induced edema and adjuvant-induced arthritis, but further investigations are required to understand the mechanism involved.
Matsuda
H
H
Faculty of Pharmaceutical Sciences, Kinki University, Osaka, Japan.
Tanaka
T
T
Kubo
M
M
D000818:Animals; D001104:Arbutin / Q000494:pharmacology / Q000627:therapeutic use*; D001169:Arthritis, Experimental / Q000188:drug therapy; D002351:Carrageenan; D003877:Dermatitis, Contact / Q000188:drug therapy*; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005260:Female; D006968:Hypersensitivity, Delayed / Q000188:drug therapy; D007213:Indomethacin / Q000494:pharmacology / Q000627:therapeutic use*; D051379:Mice; D010853:Picryl Chloride; D051381:Rats; D011919:Rats, Inbred Strains
D004365:Drugs, Chinese Herbal; D002351:Carrageenan; D001104:Arbutin; D007213:Indomethacin; D010853:Picryl Chloride