Imaging of myocardial infarction in dogs and humans using monoclonal antibodies specific for human myosin heavy chains.
The use of three different monoclonal antibodies specific for human ventricular myosin heavy chains in the visualization of the location and extent of necrosis in dogs with experimental acute myocardial infarction and in humans is described. Using a classic immunohistochemical method or ex vivo analysis of heart slices in dogs with acute myocardial infarction subjected to intravenous injection of unlabeled antimyosin antibodies or antimyosin antibodies labeled with indium-111, it was observed that all antibody fragments specifically reached the targeted necrotic zone less than 2 h after antibody injection and remained bound for up to 24 h. In a limited but significant number of cases (5 of the 12 humans and 11 of 43 dogs), it was possible to image the necrotic zone in vivo as early as 2 to 4 h after antibody injection. In other cases, individual blood clearance variations retarded or even prevented in vivo necrosis detection. Higher antimyosin fixation values were obtained in the necrotic zones in dogs with a rapid blood clearance relative to that of the other dogs. It is concluded that antimyosin antibodies always reached necrotic areas within 2 h. If blood clearance was rapid, in vivo imaging of the necrotic area was possible 2 to 6 h after necrosis, even in humans. In some cases, however, uncontrolled individual variations in the timing required for sufficient blood clearance hampered this rapid in vivo detection of myocardial necrosis.
Léger
J
J
INSERM, Faculté de Pharmacie, Montpellier, France.
Chevalier
J
J
Larue
C
C
Gautier
P
P
Planchenault
J
J
Aumaître
E
E
Messner
P
P
Puech
P
P
Saccavini
J C
JC
Pau
B
B
D000368:Aged; D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics; D004285:Dogs; D005260:Female; D006321:Heart / Q000000981:diagnostic imaging*; D006801:Humans; D007205:Indium Radioisotopes; D008297:Male; D009203:Myocardial Infarction / Q000000981:diagnostic imaging*; D009218:Myosins / Q000276:immunology*; D004369:Pentetic Acid; D011877:Radionuclide Imaging; D013997:Time Factors
D000911:Antibodies, Monoclonal; D007205:Indium Radioisotopes; D004369:Pentetic Acid; D009218:Myosins