Purification of radioiodinated monoclonal antibodies using a simple 'closed procedure' device.
An elution device has been constructed which is capable of drawing radiolabelled monoclonal antibody through a Sephadex-G chromatography column under negative pressure. Monoclonal antibodies against ovarian carcinoma and T and B lymphocytes were radioiodinated using the Chloramine-T method. Labelling efficiencies were in the range 75-85%. After passing through the chromatographic eluter no 'free' iodide was detected in the antibody samples. The apparatus was simple to use and safe to the operator.
Sampson
C B
CB
Department of Nuclear Medicine, Addenbrooke's Hospital, Cambridge, UK.
Solanki
C
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000912:Antibodies, Neoplasm / Q000302:isolation & purification; D002850:Chromatography, Gel / Q000295:instrumentation*; D004867:Equipment Design; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes / Q000302:isolation & purification*; D008214:Lymphocytes / Q000276:immunology; D010051:Ovarian Neoplasms / Q000276:immunology; D051381:Rats; D012974:Sodium Iodide
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D007457:Iodine Radioisotopes; D012974:Sodium Iodide