title: Candida albicans and three other Candida species contain an elongation factor structurally and functionally analogous to elongation factor 3.
abstract: A cell-free poly(U)-dependent translation elongation system from Candida albicans is ATP-dependent due to the presence of an elongation factor 3 (EF3)-like activity. Saccharomyces cerevisiae ribosomes added to a C. albicans postribosomal supernatant (PRS) supported poly(U)-dependent elongation, suggesting that the C. albicans lysate contained a soluble translation factor functionally analogous to the S. cerevisiae translation factor EF-3. The presence of EF-3 in C. albicans was confirmed by Western blotting using an antibody raised against S. cerevisiae EF-3. This antibody was also used to screen a selection of Candida species, all of which possessed EF-3 with molecular mass in the range of 110-130 kDa.
authors.affiliation: Biological Laboratory, University of Kent, Canterbury, U.K.
authors.forename: D R
authors.initials: DR
authors.lastname: Colthurst
authors.forename: M
authors.initials: M
authors.lastname: Santos
authors.forename: C M
authors.initials: CM
authors.lastname: Grant
authors.forename: M F
authors.initials: MF
authors.lastname: Tuite
mesh_terms: D002176:Candida albicans / Q000032:analysis* / Q000276:immunology; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D010445:Peptide Elongation Factors / Q000737:chemistry / Q000302:isolation & purification* / Q000502:physiology; D014176:Protein Biosynthesis; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D005656:Fungal Proteins; D010445:Peptide Elongation Factors; D029701:Saccharomyces cerevisiae Proteins; C111584:YEF3 protein, S cerevisiae