[The precursor hematopoietic cell: its origin in ontogeny, proliferative activity and proliferative potential].
Cells responsible for repopulation of irradiated longterm cultures of murine bone marrow and capable of generating CFUs for at least 4-5 weeks after seeding referred here to as primitive hemopoietic stem cells (P-HSC) were assayed by limiting dilution analysis. During development of mice P-HSC can be detected for the first time in the liver of 12-13-day-old embryos and their number is about 10 per organ. At day 17-18 of gestation the number of P-HSC increases ten-fold; however, we could not detect the proliferation of these cells using the technique of hydroxyurea suicide. In the adult mouse P-HSC content is about 100 precursors per femur and their concentration is one P-HSC per 1-2 x 10(5) bone marrow cells. P-HSC content in the spleen is 0.5 per 10(6) cells. In vivo treatment with 5-fluorouracil or hydroxyurea (six injections every 6 h) does not alter significantly the number of P-HSC, although either treatment kills about 99% of CFUs. Several months after reconstitution of lethally irradiated mice with a "small" inoculum of bone marrow cells (0.20-0.35 x 10(6)) the number of bone marrow P-HSC was reduced as compared to that in animals reconstituted by injection of a "large" cell dose (20-35 x 10(6)). These data suggest that P-HSC have limited proliferative potential and are incapable of self-maintenance.
Deriugina
E I
EI
Drize
N I
NI
Olovnikova
N I
NI
Sadovnikova
E Iu
EIu
Chertkov
I L
IL
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000528:radiation effects; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D003114:Colony-Forming Units Assay; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D006918:Hydroxyurea / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000196:embryology / Q000528:radiation effects; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D013997:Time Factors
D005472:Fluorouracil; D006918:Hydroxyurea
Rodonachal'naia krovetvornaia kletka: vozniknovenie v ontogeneze, proliferativnaia aktivnost' i proliferativnyĭ potentsial.