title: In vitro effect of transforming growth factor-beta on progression of HIV-1 infection in primary mononuclear phagocytes.
abstract: In vitro-differentiated monocytes can be infected with the monocytotropic isolate of HIV-1/ADA. The infection is characterized by formation of giant cells and production of virus that can be found in cell supernatants or cell-associated. In this study, we demonstrate that the above described parameters of infection can be enhanced by a factor present in acidified M phi supernatants, suggesting that it might be transforming growth factor beta-1 (TGF-beta 1). When recombinant or purified TGF-beta were examined, similar activities were detected. This effect apparently is not because of changes in the cellular phenotype that could favor infection. The effect of TGF-beta is exerted on cells once infection is established or on cells with active virus production. The activity can be also demonstrated using U-937 cells.
authors.affiliation: CIBA-GEIGY Ltd., Pharma Research, Basel, Switzerland.
authors.forename: J K
authors.initials: JK
authors.lastname: Lazdins
authors.forename: T
authors.initials: T
authors.lastname: Klimkait
authors.forename: K
authors.initials: K
authors.lastname: Woods-Cook
authors.forename: M
authors.initials: M
authors.lastname: Walker
authors.forename: E
authors.initials: E
authors.lastname: Alteri
authors.forename: D
authors.initials: D
authors.lastname: Cox
authors.forename: N
authors.initials: N
authors.lastname: Cerletti
authors.forename: R
authors.initials: R
authors.lastname: Shipman
authors.forename: G
authors.initials: G
authors.lastname: Bilbe
authors.forename: G
authors.initials: G
authors.lastname: McMaster
mesh_terms: D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D016212:Transforming Growth Factor beta