Analysis of oxygen equilibria of the giant hemoglobin from the earthworm Eisenia foetida using the Adair model.
Oxygen equilibrium curves of the giant hemoglobin from the earthworm Eisenia foetida were determined at various concentrations of cations. Using the Adair model of 12 oxygenation steps, we succeeded in fitting the data better than the simple concerted model (MWC model). Analysis of the Adair constants (K1 to K12) indicated that the increase in oxygen affinity occurs in the last six steps (K7 to K12) of the oxygen binding and that it is enhanced by increase in Ca2+ concentration. The Hill coefficient (nmax) at pH 7.5 attained a maximum value of 9.76 at 20 mM CaCl2. In the presence of physiological levels of Ca2+ (5 mM), the Bohr effect was similar to that seen in vertebrates. The data were consistent with the release of two Bohr protons being accompanied by the oxygen-linked binding of one Ca2+. Mg2+ and Na+ exerted a similar effect on the hemoglobin, though to a lesser extent. The stoichiometry of Ca2+ binding of the hemoglobin revealed the presence of two classes of binding sites, of which the affinities are high (Ka = 8.8 x 10(3) +/- 103 M-1) and low. The number of high affinity sites per heme was found to be 0.3, comparable to the number of oxygen-linked Ca2+ binding sites.
Igarashi
Y
Y
Department of Biochemistry, Dokkyo University School of Medicine, Tochigi.
Kimura
K
K
Kajita
A
A
D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000494:pharmacology*; D006454:Hemoglobins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D008954:Models, Biological; D009835:Oligochaeta / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D012964:Sodium / Q000494:pharmacology
D006454:Hemoglobins; D012964:Sodium; D008274:Magnesium; D010100:Oxygen; D002118:Calcium