Influence of antioxidants on SOD activity in bovine sperm.
SOD activity and susceptibility to peroxidation on spermatozoa from frozen and fresh bovine semen were determined either in presence or not of synthetic (BHA) or natural (vitamin E) antioxidants. In sperm suspensions incubated with vitamin E, SOD activity was higher than in the control samples and the ones treated with BHA. It was found a highly significative correlation between malondialdehyde production and SOD activity. SOD activity could be used in bovine spermatozoa as a metabolic indicator of membrane integrity.
Beconi
M T
MT
Cátedra de Química Biológica, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Argentina.
Affranchino
M A
MA
Schang
L M
LM
Beorlegui
N B
NB
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D002417:Cattle; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D013094:Spermatozoa / Q000187:drug effects* / Q000201:enzymology; D013482:Superoxide Dismutase / Q000187:drug effects*; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D014810:Vitamin E; D002083:Butylated Hydroxyanisole; D013482:Superoxide Dismutase