Evidence for sulfite as an essential metabolite for human peripheral lymphocytes.
Sulfite has been identified as an essential metabolite by means of growth studies using a chemically-defined, protein-free medium for culture of human peripheral lymphocytes. Sulfite reduced the amount of cysteine required for optimum growth by at least four-fold. In some subjects, sulfite stimulated growth even in the presence of optimal amounts of cysteine indicating that lymphocytes of some individuals are unable to convert cysteine to sulfite in adequate amounts.
Pettit
F H
FH
Clayton Foundation Biochemical Institute, Department of Chemistry, University of Texas, Austin 78712.
Lyon
D
D
Brown
J R
JR
Shive
W
W
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003545:Cysteine / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction; D013447:Sulfites / Q000378:metabolism / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D013447:Sulfites; D014316:Tritium; D003545:Cysteine; D013936:Thymidine