title: Influence of innervation on molecular forms of acetylcholinesterase in regenerating fast and slow skeletal muscles.
abstract: Nerve-intact muscle regenerates were prepared by ischemic-toxic injury of slow soleus (SOL) and fast extensor digitorum longus (EDL) muscles of the rat. Rapid innervation of regenerating myotubes modified intrinsic patterns of AChE molecular forms, revealed by velocity sedimentation in linear sucrose gradients. Regarding their onset, the effects of innervation can be classified as early and late. The earliest changes in the SOL regenerates appeared a few days after innervation by their motoneurons: the activity of the 13 S AChE form (A 8) increased significantly in comparison to non-innervated regenerates. The pattern of AChE molecular forms became similar to that in the normal SOL muscle during the 2nd week after injury. In contrast, no major differences were observed between 8 day-old innervated and non-innervated EDL regenerates. Their patterns of AChE molecular forms resembled that in the normal EDL. However, the predominance of the 10 S AChE form (G 4) characteristic for the 2-week old non-innervated regenerates was prevented by innervation. Early effect of innervation observed in the SOL regenerates but not in the EDL may be due to intrinsically different response of the regenerating SOL myotubes to innervation. Rather high extrajunctional activity of the asymmetric 16 S (A 12) molecular form of AChE in early regenerates was reduced to adult level in about 3 weeks in the SOL, and nearly completely suppressed in 5 weeks after innervation in the EDL regenerates. This reduction is assumed to be a late effect of innervation, as well as a decrease of the activity of the 4 S AChE form (G 1) in the SOL regenerates. A suppressive mechanism is activated in the extra-junctional regions of the innervated muscle regenerates during their maturation.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Institute of Pathophysiology, School of Medicine, E. Kardelj University, Ljubljana, Yugoslavia.
authors.forename: N
authors.initials: N
authors.lastname: Crne
authors.forename: J
authors.initials: J
authors.lastname: Sketelj
authors.forename: M
authors.initials: M
authors.lastname: Brzin
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis*; D000818:Animals; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D009046:Motor Neurons / Q000502:physiology*; D009119:Muscle Contraction; D009124:Muscle Proteins / Q000032:analysis*; D009132:Muscles / Q000201:enzymology / Q000293:injuries / Q000294:innervation*; D051381:Rats; D011919:Rats, Inbred Strains; D012038:Regeneration
chemical_list: D007527:Isoenzymes; D009124:Muscle Proteins; D000110:Acetylcholinesterase