Specific chromosome change associated with acquisition in vivo of tumorigenicity in carcinogen-induced rat urinary bladder carcinoma cells.
The correlation between chromosomal changes and tumorigenic potential of rat bladder epithelial cells was investigated. Seven cell lines were established from neoplastic urothelial cells derived from heterotopically transplanted rat bladders treated with topical application of one of two carcinogens, N-methyl-N-nitrosourea (MNU) or N-hydroxy-N-glucuronosyl-2-aminofluorene. Loss of the short arm of chromosome 3 was demonstrated in three of four lines tumorigenic in the nude mouse, but in none of three nontumorigenic lines. One of the three nontumorigenic cell lines (D44c) was treated further in vitro with MNU. All six tumorigenic, but none of the four nontumorigenic, morphologically altered cell lines derived from D44c demonstrated loss of the short arm of chromosome 3. These results suggest that chromosome 3 alterations may be associated with the tumorigenicity of carcinogen-induced rat bladder epithelial cells.
Debiec-Rychter
M
M
Department of Chemical Carcinogenesis, Michigan Cancer Foundation, Detroit 48201.
Azuma
M
M
Zukowski
K
K
Oyasu
R
R
Wang
C Y
CY
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D025063:Chromosome Disorders; D004171:Diploidy; D005819:Genetic Markers; D007621:Karyotyping; D008297:Male; D008770:Methylnitrosourea; D009368:Neoplasm Transplantation; D011336:Probability; D051381:Rats; D011916:Rats, Inbred F344; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology
D005819:Genetic Markers; D008770:Methylnitrosourea