title: Subunit structure of the hemocyanins of some of the Muricidae and Fasciolariidae families: Chicoreus florifer dilectus (A. Adams), Muricanthus fulvescens (Sowerby), Urosalpinx cinerea (Say), Fascilaria lilium hunteria (Perry), and Pleuroploca gigantea (Kiener).
abstract: 1. The hemocyanins of the Muricidae and Fasciolariidae families of marine gastropods: Chicoreus florifer dilectus, Muricanthus fulvescens, Urosalpinx cinerea, Fasciolaria lilium hunteria, and Pleuroploca gigantea were investigated by sedimentation velocity, scanning transmission electron microscopy, light-scattering, and other physical techniques. 2. The hemocyanins of these species are characterized by sedimentation coefficients close to 100 S and molecular weights of 8.2 x 10(6)-9.0 x 10(6). 3. The hemocyanins have di-decameric structures, with tail-to-tail arrangement of the decameric halves of the cylindrical particles. Only the hemocyanin of U. cinerea was found to contain about 30% higher, tri-, and tetra-decameric particles, with one or two decameric units added in a tail-to-head manner to a central di-decameric particle of the Mellema and Klug tail-to-tail arrangement. 4. The influence of pH, and the urea and Hofmeister salt series of reagents on the subunit structure and denaturation of P. gigantea hemocyanin were also investigated.
authors.affiliation: Department of Chemistry, Fordham University, Bronx, NY 10458.
authors.forename: T T
authors.initials: TT
authors.lastname: Herskovits
authors.forename: J A
authors.initials: JA
authors.lastname: Gonzalez
authors.forename: M G
authors.initials: MG
authors.lastname: Hamilton
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002942:Circular Dichroism; D006433:Hemocyanins / Q000737:chemistry* / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D008027:Light; D046911:Macromolecular Substances; D008855:Microscopy, Electron, Scanning; D008974:Mollusca / Q000032:analysis*; D011489:Protein Denaturation; D012542:Scattering, Radiation; D014461:Ultracentrifugation; D014508:Urea; D014783:Viscosity
chemical_list: D046911:Macromolecular Substances; D014508:Urea; D006433:Hemocyanins