Highly sensitive detection of beta-2 transferrin in rhinorrhea and otorrhea as a marker for cerebrospinal fluid (C.S.F.) leakage.
As beta-2 transferrin is almost specific to the cerebrospinal fluid, its detection in rhinorrhea or otorrhea fluids demonstrates the occurrence of CSF fistula. We describe a highly sensitive method based on immunoaffinity-mediated capillary blotting for the detection of this cathodic isoform of transferrin in minute amounts (3 microliters) of rhinorrhea or otorrhea fluids. Application of this method in a series of 10 patients with CSF fistula is reported.
Fransen
P
P
Unité de Neurochirurgie, Cliniques Universitaires Saint-Luc, Bruxelles, Belgium.
Sindic
C J
CJ
Thauvoy
C
C
Laterre
C
C
Stroobandt
G
G
D000293:Adolescent; D000328:Adult; D002558:Cerebrospinal Fluid Otorrhea / Q000134:cerebrospinal fluid*; D002559:Cerebrospinal Fluid Rhinorrhea / Q000134:cerebrospinal fluid*; D006801:Humans; D008875:Middle Aged; D014168:Transferrin / Q000134:cerebrospinal fluid*
D014168:Transferrin