Preliminary X-ray crystallographic investigation of human parvovirus B19.
Crystals that diffract X rays to at least 8 A resolution have been grown from human B19 parvovirus empty capsids. These particles consist of VP-2 derived from a baculovirus expression system. This is possibly the first time that a self-assembled empty viral capsid, grown in other than normal host cells, has been crystallized. Partial X-ray diffraction data have been collected using synchrotron radiation. The space group is P2(1)3 with a = 362 A. The particle position in the crystal cell is given, at least roughly, from packing considerations.
Agbandje
M
M
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
McKenna
R
R
Rossmann
M G
MG
Kajigaya
S
S
Young
N S
NS
D000818:Animals; D002460:Cell Line; D005814:Genes, Viral; D006801:Humans; D007313:Insecta; D008854:Microscopy, Electron; D010321:Parvoviridae / Q000235:genetics / Q000648:ultrastructure*; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D014961:X-Ray Diffraction / Q000379:methods
D012333:RNA, Messenger