title: Assay of HIV-1 proteinase: a colorimetric method using small peptide substrates.
abstract: A colorimetric assay for HIV proteinase using small protected peptide substrates is described. Substrates are cleaved to release N-terminal prolyl peptides which react with isatin to form a blue product which is measured spectrophotometrically. The assay is suitable for use with pure enzyme or crude extracts derived from genetically engineered Escherichia coli.
authors.affiliation: Department of Chemotherapy Biology, Roche Products Ltd., Welwyn Garden City, Hertfordshire, United Kingdom.
authors.forename: A V
authors.initials: AV
authors.lastname: Broadhurst
authors.forename: N A
authors.initials: NA
authors.lastname: Roberts
authors.forename: A J
authors.initials: AJ
authors.lastname: Ritchie
authors.forename: B K
authors.initials: BK
authors.lastname: Handa
authors.forename: C
authors.initials: C
authors.lastname: Kay
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology; D016333:HIV Protease / Q000032:analysis*; D007510:Isatin / Q000737:chemistry; D007700:Kinetics; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000032:analysis* / Q000138:chemical synthesis; D011392:Proline / Q000032:analysis; D013379:Substrate Specificity
chemical_list: D009842:Oligopeptides; D007510:Isatin; D011392:Proline; D016333:HIV Protease