title: Variations in the cytoplasmic region account for the heterogeneity of the chicken MHC class I (B-F) molecules.
abstract: Molecular variation among major histocompatibility complex (MHC) class I (B-F) proteins from B-homozygous chickens is apparently caused by C-terminal variation. Analysis of the total B-F protein pool revealed substantial heterogeneity with two or three molecular mass constituents, each being comprised by several isoelectric focusing variants. This heterogeneity could not be reduced by enzymatic deglycosylation. By contrast, proteolytic removal of a small (Mr 1000-4000) fragment from the alpha chain resulted in the generation of a Mr 36,000 fragment, common to all the molecular mass variants. Unlike the parent proteins, the Mr 36,000 fragment derived from isolated variants yielded identical, simple patterns in two-dimensional gel electrophoresis and identical finger prints in peptide mapping. This, together with N-terminal amino acid sequencing, as well as comparison of hydrophobicity properties of fragments obtained by gradual proteolytic digestion, indicated that the small peptide responsible for the major B-F heterogeneity was situated in the intracellular, C-terminal part.
authors.affiliation: Institute for Experimental Immunology, University of Copenhagen, Denmark.
authors.forename: L B
authors.initials: LB
authors.lastname: Møller
authors.forename: J
authors.initials: J
authors.lastname: Kaufman
authors.forename: S
authors.initials: S
authors.lastname: Verland
authors.forename: J
authors.initials: J
authors.lastname: Salomonsen
authors.forename: D
authors.initials: D
authors.lastname: Avila
authors.forename: J D
authors.initials: JD
authors.lastname: Lambris
authors.forename: K
authors.initials: K
authors.lastname: Skjødt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002645:Chickens / Q000276:immunology*; D003593:Cytoplasm / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005805:Genes, MHC Class I; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis
chemical_list: D002241:Carbohydrates; D015395:Histocompatibility Antigens Class I; D010446:Peptide Fragments