Chronic mianserin treatment decreases 5-HT2 receptor binding without altering 5-HT2 receptor mRNA levels.
Rats were injected with 15 mg/kg (i.p.) mianserin or vehicle (saline) for 4, 10 or 21 days and 5-HT2 receptor binding and mRNA levels measured. Treatment with mianserin induced a substantial decrease in 5-HT2 radioligand binding (44-59% decrease; P less than 0.05 vs. control). No changes in the amount of 5-HT2 or, as a control, beta-actin mRNAs were found (P greater than 0.05 vs. control). These results indicate that mianserin decreases 5-HT2 radioligand binding without altering steady-state 5-HT2 mRNA levels.
Roth
B L
BL
Nancy Pritzker Laboratory of Molecular and Developmental Neurobiology, Department of Psychiatry and Behavioural Sciences, Stanford University Medical Center, CA 94305.
Ciaranello
R D
RD
D000818:Animals; D015152:Blotting, Northern; D015342:DNA Probes; D008297:Male; D008803:Mianserin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D011985:Receptors, Serotonin / Q000187:drug effects* / Q000235:genetics
D015342:DNA Probes; D012333:RNA, Messenger; D011985:Receptors, Serotonin; D008803:Mianserin