Determination of proteins in urine by high-performance liquid chromatography with spectrophotometric detection using a pyrogallol red-molybdate complex.
A high-performance liquid chromatographic method with spectrophotometric detection was developed for the determination of proteins in urine. The proteins were separated on an anion-exchange column and eluted with a Tris-HCl buffer with a gradient of sodium chloride concentration and pH. The separated proteins were mixed with a pyrogallol red-molybdate complex reagent and determined spectrophotometrically. Urinary proteins were well separated without desalting the urine. The reproducibility was satisfactory.
Mashige
F
F
Department of Laboratory Medicine, Faculty of Medicine, University of Tokyo, Japan.
Ohkubo
A
A
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008982:Molybdenum; D011507:Proteinuria / Q000378:metabolism*; D011748:Pyrogallol / Q000031:analogs & derivatives; D012177:Retinol-Binding Proteins / Q000652:urine; D012965:Sodium Chloride; D013053:Spectrophotometry / Q000379:methods*; D001613:beta 2-Microglobulin / Q000652:urine
D012177:Retinol-Binding Proteins; D001613:beta 2-Microglobulin; D011748:Pyrogallol; C044659:molybdate; C058731:pyrogallol sulfonphthalein; D012965:Sodium Chloride; D008982:Molybdenum