[The therapeutic use of diucifon-activated immunocytes in adoptive transfer in mice with burn trauma complicated by Pseudomonas aeruginosa infection].
The effectiveness of the therapeutic use of diucyphone-stimulated syngeneic spleen cells was studied in mice with a burn trauma complicated by P. aeruginosa infection. For this purpose CBA mice were subjected to a burn of degree IIIa, covering 30% of their body surface, the wound was infected with P. aeruginosa strain, and 24 hours later spleen cells were taken from some of these animals. The spleen cells, incubated for 3 hours with or without diucyphone at a concentration of 10-100 micrograms/ml, were washed and reinfused intravenously to the remaining animals. The injection of diucyphone-treated spleen cells was shown to greatly increase the survival rate of the animals, to accelerate healing processes and to enhance the proliferative response of spleen cells to mitogens (phytohemagglutinin, concanavalin A) and interleukin-2. A conclusion was made on the efficacy of the therapeutic use of diucyphone-activated immunocompetent cells in cases of immunodeficiency induced by burn infection.
Prozorovskiĭ
N S
NS
Radkevich
S A
SA
Radchenkov
V P
VP
Moroz
A F
AF
Leskov
V P
VP
Gushchin
I S
IS
D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000818:Animals; D002056:Burns / Q000150:complications / Q000276:immunology / Q000401:mortality / Q000628:therapy*; D004338:Drug Combinations; D004353:Drug Evaluation, Preclinical; D016219:Immunotherapy, Adoptive / Q000379:methods*; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000276:immunology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D011552:Pseudomonas Infections / Q000209:etiology / Q000276:immunology / Q000401:mortality / Q000628:therapy*; D013154:Spleen / Q000276:immunology; D013450:Sulfones / Q000627:therapeutic use*; D014498:Uracil / Q000031:analogs & derivatives* / Q000627:therapeutic use
D000276:Adjuvants, Immunologic; D004338:Drug Combinations; D013450:Sulfones; C023367:diucifon; D014498:Uracil
Lechebnoe primenenie aktivirovannykh diutsifonom immunotsitov v usloviiakh adoptivnogo perenosa u mysheĭ s ozhogovoĭ travmoĭ, oslozhnennoĭ sinegnoĭnoĭ infektsieĭ.