title: Immunofluorescent study on the interaction between collagen and calcium oxalate crystals in the renal tubules.
abstract: The interaction of calcium oxalate crystals and renal tubular cells was studied. Rat renal collecting tubular cells were cultured and immunologically stained with anti-type-IV collagen antiserum (type-IV collagen exists in renal tubular basement membrane). When renal tubular cells and calcium oxalate crystals were mixed, clumps were formed. These clumps were examined by immunological staining with anti-type-IV collagen antiserum. In another series of experiments, calcium-containing crystals were found to be adsorbed onto mucous threads and cast-like materials, although no such adsorption was observed on squamous cells. These absorbed materials interacted with anti-type-IV collagen antiserum. These results suggest that collagen in the renal tubular basement membrane may act as matrix in urinary stone formation.
authors.affiliation: Department of Urology, Kinki University School of Medicine, Osaka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kohri
authors.forename: M
authors.initials: M
authors.lastname: Kodama
authors.forename: Y
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Katayama
authors.forename: H
authors.initials: H
authors.lastname: Matsuda
authors.forename: M
authors.initials: M
authors.lastname: Imanishi
authors.forename: M
authors.initials: M
authors.lastname: Takada
authors.forename: Y
authors.initials: Y
authors.lastname: Katoh
authors.forename: K
authors.initials: K
authors.lastname: Kataoka
authors.forename: T
authors.initials: T
authors.lastname: Akiyama
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D002129:Calcium Oxalate / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism*; D003460:Crystallization; D005455:Fluorescent Antibody Technique; D007684:Kidney Tubules / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D051381:Rats; D014545:Urinary Calculi / Q000209:etiology*
chemical_list: D002129:Calcium Oxalate; D003094:Collagen