title: Immunochemical identification of branched-chain 2-oxo acid dehydrogenase kinase.
abstract: Branched-chain 2-oxo acid dehydrogenase kinase was characterized using anti-kinase polyclonal antibodies. The antibodies were purified from rabbit antiserum by an epitope selection method. The antibodies bound only to a 44 kDa polypeptide in the dehydrogenase-kinase complex and inhibited the kinase activity, substantiating that the 44 kDa polypeptide is the catalytic subunit of the kinase. The purified liver dehydrogenase-kinase complex, but not either the purified heart complex or the partially purified liver complex, contained 2 additional polypeptides of lower molecular weight which also reacted with the anti-kinase antibodies, suggesting that the liver kinase is subject to proteolytic degradation during purification.
authors.affiliation: Laboratory of Biochemistry of Exercise and Nutrition, University of Tsukuba, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shimomura
authors.forename: N
authors.initials: N
authors.lastname: Nanaumi
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: R A
authors.initials: RA
authors.lastname: Harris
mesh_terms: D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D007658:Ketone Oxidoreductases / Q000276:immunology / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D009097:Multienzyme Complexes / Q000276:immunology / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology; D011494:Protein Kinases / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal; D009097:Multienzyme Complexes; D007658:Ketone Oxidoreductases; D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D011494:Protein Kinases