The effect of size of polystyrene particles on their retention within the rat peritoneal compartment, and on their interaction with rat peritoneal macrophages in vitro.
Polystyrene particles (size range 300 nm-3 microns diameter) were radioiodinated and their capture by rat peritoneal macrophages measured in vitro. For unmodified particles, most efficient accumulation was observed using a diameter of 600 nm (Endocytic Index (E.I.) = 16.4 +/- 2.9 microliters/10(6) cells/h). Particles (3 microns diameter) which had been modified to become more hydrophilic by hydroxymethylation showed an increased rate of capture (E.I. = 136.6 +/- 91.2 microliters/10(6) cells/h). Following intraperitoneal administration to rats, unmodified 3 micron particles showed selective accumulation in the omentum (18.4% injected dose/g), and this was increased for the hydroxymethylated bead (35.3% dose/g). The smaller (800 nm) particles were better able to leave the peritoneal compartment. Radiolabelled particles isolated from a peritoneal wash after 5 h were mostly cell-associated (72-86%, depending on the type of particle).
Seymour
L
L
Cancer Research Campaign Laboratories, University of Keele, Staffs, UK.
Schacht
E
E
Duncan
R
R
D000818:Animals; D001202:Ascitic Fluid / Q000378:metabolism*; D004337:Drug Carriers; D066298:In Vitro Techniques; D007274:Injections, Intraperitoneal; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D010316:Particle Size; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis; D011137:Polystyrenes / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051381:Rats; D011919:Rats, Inbred Strains
D004337:Drug Carriers; D011137:Polystyrenes