title: Effects of intracerebroventricularly administered neostigmine on sympathetic neural activities of peripheral tissues in rats.
abstract: Sympathetic nervous activity (SNA) in the liver, heart, pancreas and interscapular brown adipose tissue was examined 60 min after the third cerebroventricular injection of neostigmine (5 x 10(-8) mol) in rats. We employed the technique of specific gas chromatography-mass spectrometry for simultaneous analysis of norepinephrine (NE) and its primary neuronal metabolite, 3,4-dihydroxyphenylethyleneglycol (DHPG) and used the ratio DHPG/NE as an index of SNA. Neostigmine produced significant increases in the DHPG/NE ratio in all tissues investigated. Co-administration of atropine with neostigmine completely inhibited this neostigmine-induced effect. These findings suggest that the central cholinergic-muscarinic activation with neostigmine stimulates SNA in the peripheral tissues examined.
authors.affiliation: Garvan Institute of Medical Research, St. Vincent's Hospital, Sydney, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Gotoh
authors.forename: G A
authors.initials: GA
authors.lastname: Smythe
mesh_terms: D002001:Adipose Tissue, Brown / Q000294:innervation; D000818:Animals; D001285:Atropine / Q000008:administration & dosage / Q000494:pharmacology; D002552:Cerebral Ventricles / Q000187:drug effects / Q000502:physiology*; D006329:Heart Conduction System / Q000187:drug effects; D007276:Injections, Intraventricular; D008099:Liver / Q000294:innervation; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000378:metabolism; D009388:Neostigmine / Q000008:administration & dosage / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009638:Norepinephrine / Q000378:metabolism; D009928:Organ Specificity; D010179:Pancreas / Q000294:innervation; D051381:Rats; D011919:Rats, Inbred Strains; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000502:physiology*
chemical_list: D009388:Neostigmine; D008734:Methoxyhydroxyphenylglycol; D001285:Atropine; C010117:3,4-dihydroxyphenylglycol; D009638:Norepinephrine