[Changes in the profile of cytotoxic mediator monocytes in patients with cancer and precancerous conditions of the stomach].
Peripheral blood monocytes from healthy subjects, patients with gastric precancer disease (chronic gastric ulcer, stomach polyps and chronic atrophic gastritis) and different stages of gastric cancer were used. Spontaneous and lipopolysaccharide (LPS)-stimulated TNF-like factors production by monocytes was significantly higher in the precancer gastric disease patients than in the healthy subjects. At the same time the spontaneous capacity of monocytes to produce NTF-like factors was 2.5 lower in the gastric cancer patients compared to the healthy subjects. Moreover, in 5/13 of the gastric cancer patients in TNF-like factors production by the LPS-stimulated and non-stimulated monocytes was 1 unit/ml less. Spontaneous and reactive CL indexes were higher in the cancer patients monocytes than in the healthy subjects. The obtained results suggest that reactive oxygen species production can be an alternative mechanism by which a cytotoxic action of monocytes is regulated.
Shepetkin
I A
IA
Volyntseva
I B
IB
Borunov
E V
EV
Antipova
E A
EA
Naumov
S A
SA
Udut
V V
VV
D000328:Adult; D002469:Cell Separation; D002478:Cells, Cultured / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000276:immunology* / Q000502:physiology; D004926:Escherichia coli; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D016260:Monocytes, Activated Killer / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D010100:Oxygen / Q000097:blood; D011230:Precancerous Conditions / Q000097:blood / Q000276:immunology*; D013274:Stomach Neoplasms / Q000097:blood / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000096:biosynthesis
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D010100:Oxygen
Izmenenie profilia tsitotoksicheskikh mediatorov monotsitov u bol'nykh rakom i predrakovymi sostoianiiami zheludka.