Expression strategy of the potato virus X triple gene block.
The mode of expression of the overlapping genes of the triple block positioned internally in potato virus X (PVX) RNA was examined. The results of In vitro translation of synthetic RNA transcripts and natural PVX-specific methylmercuric hydroxide-denatured dsRNAs suggest that the 25K protein is expressed as a single translation product of the 2.1 kb subgenomic (sg) RNA and that both the 12K and 8K proteins are expressed from the same 1.4 kb sgRNA.
Morozov SYu
A. N. Belozersky Laboratory of Molecular Biology, Moscow State University, U.S.S.R.
Miroshnichenko
N A
NA
Solovyev
A G
AG
Fedorkin
O N
ON
Zelenina
D A
DA
Lukasheva
L I
LI
Karasev
A V
AV
Dolja
V V
VV
Atabekov
J G
JG
D001483:Base Sequence; D004279:DNA, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D015967:Gene Expression Regulation, Viral; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000235:genetics*; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D011198:Solanum tuberosum / Q000382:microbiology
D004279:DNA, Viral; D012367:RNA, Viral