Fluorescence changes of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole bound to thiol groups of gizzard myosin.
The fluorescence intensity of gizzard myosin that incorporated about 2 mol of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-C1) at 0.6 M KC1 was quenched at low concentrations of MgATP or in the presence of F-actin. The observed quenching was reduced at high MgATP concentrations. The half maximal change in quenched fluorescence was shifted from 7.5 microM to 25 microM MgATP for NBD-myosin at 0.15 M KC1. The fluorescent label NBD-C1 can be used to detect changes in the conformation of gizzard myosin.
Bailin
G
G
Department of Molecular Biology UMDNJ-School of Osteopathic Medicine, Stratford 08084.
Huang
J R
JR
D009327:4-Chloro-7-nitrobenzofurazan / Q000737:chemistry* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D005456:Fluorescent Dyes; D000070559:Gizzard, Non-avian; D009218:Myosins / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D000255:Adenosine Triphosphate; D009218:Myosins; D009327:4-Chloro-7-nitrobenzofurazan