title: [Isolation of bilirubin oxidase from Myrothecium verrucaria and the optimum conditions of enzyme production].
abstract: A bilirubin oxidase (EC 1.3.3.5) producing strain, Mv 2.1089, was isolated from several strains of Myrothecium verrucaria by dilution method. The optimum conditions of enzyme production were investigated and the results were as follows: the suitable medium was cultured at 25 degrees C on a rotating shaker glucose and peptone, at pH 6.0. The strain was cultured at 25 degrees C on a rotating shaker (150 r/min) for 96 h. Bilirubin oxidase with 0.5-1.5 u/ml was obtained in the culture medium.
authors.affiliation: National Center for Clinical Laboratory, Beijing.
authors.forename: J
authors.initials: J
authors.lastname: Guo
authors.forename: S
authors.initials: S
authors.lastname: Tao
authors.forename: P S
authors.initials: PS
authors.lastname: Mo
authors.forename: X
authors.initials: X
authors.lastname: Liang
authors.forename: G
authors.initials: G
authors.lastname: Li
mesh_terms: D003904:Mitosporic Fungi / Q000201:enzymology* / Q000302:isolation & purification; D010088:Oxidoreductases / Q000302:isolation & purification*; D044925:Oxidoreductases Acting on CH-CH Group Donors
chemical_list: D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase