title: A quantitative study of the ciliary area of experimental otitis media with effusion in cats.
abstract: A quantitative electron microscopic study was performed to determine the extent of the ciliary surface area of the middle ear epithelium in cats. In normal cats, 97.8% of the middle ear mucosa in the hypotympanum and 45.9% in the upper part of the bulla were covered by cilia. Eustachian tube obstruction (ETO) significantly reduced the ciliary area to 73.1% in the hypotympanum and to 16.2% in the upper part of bulla at 1 week after ETO. Instillation of Escherichia coli lipopolysaccharide (LPS) or cat polymorphonuclear leukocyte (PMN) lysate into the middle ear cavity followed by ETO did not reduce the ciliary area, compared with ETO induced alone. Our results indicate that ETO reduces the ciliary area of the middle ear mucosa and that LPS or neutrophils do not enhance reduction of the ciliary area compared to ETO induced alone in this experimental condition.
authors.affiliation: Department of Otorhinolaryngology, Mie University School of Medicine, Tsu, Japan.
authors.forename: C S
authors.initials: CS
authors.lastname: Jin
authors.forename: Y
authors.initials: Y
authors.lastname: Majima
authors.forename: Y
authors.initials: Y
authors.lastname: Hamaguchi
authors.forename: K
authors.initials: K
authors.lastname: Takeuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Sakakura
authors.forename: S K
authors.initials: SK
authors.lastname: Juhn
mesh_terms: D000818:Animals; D002415:Cats; D002923:Cilia / Q000187:drug effects / Q000648:ultrastructure; D004432:Ear, Middle / Q000648:ultrastructure; D004848:Epithelium / Q000648:ultrastructure; D004926:Escherichia coli; D005064:Eustachian Tube / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D009504:Neutrophils / Q000276:immunology; D010034:Otitis Media with Effusion / Q000209:etiology / Q000473:pathology*
chemical_list: D008070:Lipopolysaccharides