Correlation of biochemical blocks and genetic lesions in leucine auxotrophic strains of the imperfect yeast Candida maltosa.
The four enzymatic steps in the conversion of alpha-ketoisovaleriate to leucine were examined in the wild type and in 13 leucine auxotrophic strains of Candida maltosa. The genetic lesions in the auxotrophs, involve at least five different loci and are correlated with three enzymatic steps. This was confirmed by gene cloning, protoplast fusion, and enzyme assays. The pathway for leucine biosynthesis in C. maltosa shows general similarity to that of other lower eukaryotes but there are individual differences in the numbers of genes responsible for single enzymatic steps. A disomic state of the chromosomes carrying genes coding for alpha-isopropylmalate synthase and beta-isopropyl-malate dehydrogenase was elucidated.
Becher
D
D
Fachrichtung Biologie, Ernst-Moritz-Arndt-Universität Greifswald, FRG.
Wedler
H
H
Schulze
H
H
Bode
R
R
Kasüske
A
A
Samsonova
I
I
D015079:2-Isopropylmalate Synthase / Q000235:genetics; D001345:Autoradiography; D002175:Candida / Q000235:genetics*; D003001:Cloning, Molecular; D015342:DNA Probes; D004271:DNA, Fungal / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005800:Genes, Fungal; D007621:Karyotyping; D007930:Leucine / Q000378:metabolism*; D009154:Mutation; D010957:Plasmids; D015183:Restriction Mapping
D015342:DNA Probes; D004271:DNA, Fungal; D015079:2-Isopropylmalate Synthase; D007930:Leucine