L-alanine transport in isolated cells of interscapular brown adipose tissue in rat.
The pattern of L-alanine uptake in isolated cells of interscapular brown adipose tissue has been determined. The uptake can be divided into the diffusion component (Kd = 0.55 min-1) and a saturable Na(+)-dependent transport (KM = 0.87 mM and Vmax = 155 nmol/min/10(6) cells). The saturable component can be subdivided into MeAIB-sensitive (KM = 1.63 mM and Vmax = 162 nmol/min/10(6) cells) and MeAIB-insensitive (KM = 3.2 mM and Vmax = 39.5 nmol/min/10(6) cells). This kinetic pattern could indicate the presence of transport system (s) that resemble the commonly described transport systems for alanine uptake in several tissues.
Rodríguez-Martín
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Departament de Boquímica i Fisiologia, Universitat de Barcelona, Spain.
Remesar
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D002001:Adipose Tissue, Brown / Q000166:cytology / Q000378:metabolism*; D000409:Alanine / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000494:pharmacology*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D003545:Cysteine / Q000494:pharmacology; D005260:Female; D005973:Glutamine / Q000494:pharmacology; D007700:Kinetics; D007930:Leucine / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012540:Scapula; D012964:Sodium / Q000378:metabolism
D000621:Aminoisobutyric Acids; D005973:Glutamine; C017911:2-(methylamino)isobutyric acid; D012964:Sodium; D007930:Leucine; D003545:Cysteine; D000409:Alanine; D011188:Potassium