title: Testosterone inhibits gonadotropin-releasing hormone pulse frequency in the male sheep.
abstract: The objective was to determine the effect of chronic testosterone (T) treatment on GnRH and LH secretion in wethers. Rams were either castrated only or castrated and immediately treated with Silastic implants containing T. Several weeks later, a device for collecting hypophyseal-portal blood was surgically implanted. Six to seven days later, blood samples were collected simultaneously and continuously from the portal vessels and jugular vein of pairs of conscious animals. Samples were divided at 10-min intervals for 6-12 h. One hour before the end of collection, all animals received i.v. injections of 250 ng of GnRH. In samples collected simultaneously from 6 pairs of animals, T reduced the frequency of both GnRH pulses (1.8 +/- 0.2 vs. 0.9 +/- 0.3/h, p less than 0.03) and LH pulses (1.6 +/- 0.1 vs. 0.8 +/- 0.3/h, p less than 0.03). T did not alter amplitude of either GnRH or LH pulses. Testosterone reduced mean GnRH (9.7 +/- 0.6 vs. 7.9 +/- 0.5 pg/ml, p less than 0.05), whereas mean LH was not significantly reduced (9.6 +/- 1.4 vs. 6.1 +/- 1.8 ng/ml, p = 0.16). These results support the hypothesis that T reduces GnRH pulse frequency.
authors.affiliation: Department of Veterinary Biosciences, University of Illinois, Urbana 61801.
authors.forename: G L
authors.initials: GL
authors.lastname: Jackson
authors.forename: D
authors.initials: D
authors.lastname: Kuehl
authors.forename: T J
authors.initials: TJ
authors.lastname: Rhim
mesh_terms: D000818:Animals; D007987:Gonadotropin-Releasing Hormone / Q000097:blood*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D009919:Orchiectomy; D012756:Sheep / Q000378:metabolism*; D013737:Testis / Q000378:metabolism / Q000502:physiology; D013739:Testosterone / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D007986:Luteinizing Hormone