A highly sensitive method for quantitative analysis of phospholipid molecular species by high-performance liquid chromatography.
A highly sensitive method was developed for quantitative analysis of phospholipid molecular species. Diradylglycerols prepared from phospholipids with phospholipase C were converted to the anthroyl-diradylglycerol derivatives, which could be separated into molecular species and sensitively quantified by reverse-phase HPLC using a fluorescence detector. All the molecular species of the derivatives had the same peak area per mole, and the peak areas were proportional to the amounts of the derivatives. Quantification could be carried out at the femtomole level.
Takamura
H
H
Research Institute for Food Science, Kyoto University.
Kito
M
M
D002851:Chromatography, High Pressure Liquid; D004075:Diglycerides / Q000378:metabolism; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D010743:Phospholipids / Q000737:chemistry*; D012680:Sensitivity and Specificity; D010738:Type C Phospholipases / Q000378:metabolism
D004075:Diglycerides; D010743:Phospholipids; C035561:diarachidonyl diglyceride; D010738:Type C Phospholipases