Cloning of alginate lyase gene (alxM) and expression in Escherichia coli.
The alxM gene encoding a D-mannuronan-specific alginate lyase has been cloned from a marine bacterium isolated as an epiphyte on the brown alga, Sargassum fluitans. Expression of this gene in Escherichia coli provides a source of this enzyme for probing alginate structure and modifying the mannuronan-rich alginate polymers produced by bacterial pathogens.
Brown
B J
BJ
Department of Chemistry, Michigan Technological University, Houghton 49931.
Preston
J F
JF
Ingram
L O
LO
D015139:Blotting, Southern; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D006090:Gram-Negative Bacteria / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D000459:Phaeophyceae; D011133:Polysaccharide-Lyases / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D013379:Substrate Specificity
D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase