Calcium uptake by leukemic and normal T-lymphocytes exposed to low frequency magnetic fields.
Calcium-ion uptake by normal and leukemia lymphocytes increased during a 30-min exposure to a 13.6 Hz, sinusoidal magnetic field at 20 microT peak. The time-varying field was horizontal and parallel to a 16.5 microT component of the ambient static magnetic field. The uptake of 45Ca2+ increased 102% in a line of murine, cytotoxic T-lymphocytes (C57B1/6-derived CTLL-1), increased 126% in freshly-isolated spleen lymphocytes (C57B1/6 mice), and increased 75% in a line of lymphoma cells (C57B1/6-derived EL4). In contrast, there was no effect when the same field was applied for 30 min immediately before--as opposed to during--incorporation of calcium ions. When spleen lymphocytes were exposed during incubation with 45Ca2+ to a 60 Hz magnetic field at 20 microT peak, a small but statistically significant increase (37%) in uptake of the labeled ions occurred. These results indicate that weak, alternating magnetic fields might affect calcium-dependent functions of normal and leukemic lymphocytes.
Lyle
D B
DB
Center for Devices and Radiological Health, Food and Drug Administration, Rockville, Maryland.
Wang
X H
XH
Ayotte
R D
RD
Sheppard
A R
AR
Adey
W R
WR
D000818:Animals; D002118:Calcium / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D008223:Lymphoma / Q000378:metabolism / Q000473:pathology*; D008280:Magnetics; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000378:metabolism*; D013602:T-Lymphocytes, Cytotoxic / Q000378:metabolism*
D002118:Calcium