title: Effect of cold acclimation on GSH, antioxidant enzymes and lipid peroxidation in brown adipose tissue.
abstract: Cold acclimation increased the activities of superoxide dismutase, catalase, total and selenium (Se)-dependent glutathione peroxidases (GPx) and glutathione reductase by 2-4-fold in the brown adipose tissue (BAT) of cold-acclimated rats. Nevertheless, when expressed per unit protein, the antioxidant enzyme activities were unaltered. Sensitivity to lipid peroxidation and GSH levels both increased by one order of magnitude in the cold on a per weight basis and were still 3-5 times greater in the cold when expressed per mg of protein. We suggest that activation of BAT leads to a large increase in the potential for lipid peroxidation and that the tissue responds to this challenge by increasing practically all of its antioxidant defences. Nevertheless, GSH, and possibly GPx activity, seem to be the principal defences involved in adaptation of the tissue to a higher sensitivity to peroxidative damage after activation.
authors.affiliation: Departamento de Biología Animal-II, Fisiología Animal, Facultad de Biología, Universidad Complutense, Madrid, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Barja de Quiroga
authors.forename: M
authors.initials: M
authors.lastname: López-Torres
authors.forename: R
authors.initials: R
authors.lastname: Pérez-Campo
authors.forename: M
authors.initials: M
authors.lastname: Abelenda
authors.forename: M
authors.initials: M
authors.lastname: Paz Nava
authors.forename: M L
authors.initials: ML
authors.lastname: Puerta
mesh_terms: D000064:Acclimatization; D002001:Adipose Tissue, Brown / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D002374:Catalase / Q000378:metabolism*; D003080:Cold Temperature; D005978:Glutathione / Q000031:analogs & derivatives / Q000378:metabolism*; D019803:Glutathione Disulfide; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005980:Glutathione Reductase / Q000378:metabolism*; D015227:Lipid Peroxidation; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
chemical_list: D002374:Catalase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione; D019803:Glutathione Disulfide