Cyclical changes in pH in bovine developing enamel as sequential bands.
Developing enamel was stained with pH indicators and a banded colour pattern exhibiting alternate acidic (5.8-6.0) and neutral (7.0-7.2) staining was clearly visualized. The pH values of the enamel samples scraped from respective bands were confirmed by measuring them in suspensions with a pH meter. Neutral bands corresponded to red stripes of glyoxal bis(2-hydroxyanil) staining and acidic bands to unstained ones, suggested a correlation of the acidic and neutral stripes with the zones of ruffle-ended and smooth-ended ameloblasts.
Sasaki
S
S
Department of Biochemistry, School of Dentistry, Tokyo Medical and Dental University, Japan.
Takagi
T
T
Suzuki
M
M
D000566:Amelogenesis / Q000502:physiology*; D000627:Aminophenols; D000818:Animals; D001391:Azo Compounds; D001979:Bromthymol Blue; D002417:Cattle; D004396:Coloring Agents; D003743:Dental Enamel / Q000033:anatomy & histology / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents
D000627:Aminophenols; D001391:Azo Compounds; D004396:Coloring Agents; D007202:Indicators and Reagents; C008492:methyl red; C007899:glyoxal bis(2-hydroxyanil); D001979:Bromthymol Blue