Reduced levels of mRNA for myofibrillar proteins in skeletal muscle from septic rats.
The influence of sepsis on transcription of myofibrillar proteins in skeletal muscle was studied in rats. Sepsis was induced by cecal ligation and puncture (CLP); control rats were sham-operated. Sixteen hours later, muscle levels of mRNA for myofibrillar proteins were determined by using cDNA probes specific for transcripts for alpha actin and myosin heavy chain. Sepsis resulted in a 2-6 fold decrease in alpha actin mRNA levels and an even more pronounced reduction in myosin heavy chain mRNA levels. Results suggest that sepsis-induced reduction of muscle protein synthesis is at least partly regulated at the transcriptional level.
Hasselgren
P O
PO
Department of Surgery University of Cincinnati, Ohio 45267-0558.
Tricoli
J V
JV
Wieczorek
D
D
Steigerwald
K A
KA
Angerås
U
U
Hall-Angerås
M
M
Fischer
J E
JE
D000199:Actins / Q000235:genetics; D000818:Animals; D001424:Bacterial Infections / Q000235:genetics*; D015152:Blotting, Northern; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis*; D009218:Myosins / Q000235:genetics; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic
D000199:Actins; D015342:DNA Probes; D009124:Muscle Proteins; D012333:RNA, Messenger; D004247:DNA; D009218:Myosins