Cytochemical and immunocytochemical characterization of Yoshida ascites sarcoma cells.
Some cytochemical and immunocytochemical investigations were carried out on actively growing Yoshida ascites sarcoma cells. These cells displayed an intense granular alpha-naphthylacetate esterase (ANAE) staining while the alpha-naphthylbutyrate esterase (ANBE) reaction was in part fluoride-sensitive and marked particularly in the large-size malignant cells. Acid phosphatase as well as peroxidase activities were not detected. The lack of immunoreactive lysozyme and alpha 1-antitrypsin suggested a poor differentiation of the above-mentioned tumor cells, but fibronectin and S-100 protein where highly expressed, as in tumors arising from the mononuclear phagocyte system.
Nicotina
P A
PA
Department of Human Pathology, University of Messina, Italy.
Ruggeri
P
P
Ferlazzo
G
G
Fimiani
V
V
D000818:Animals; D001201:Ascites; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry; D005260:Female; D005353:Fibronectins / Q000737:chemistry; D008297:Male; D009283:Naphthol AS D Esterase / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains; D009418:S100 Proteins / Q000737:chemistry; D012517:Sarcoma, Yoshida / Q000737:chemistry* / Q000276:immunology
D005353:Fibronectins; D009418:S100 Proteins; D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases; C021855:naphthylbutyrate esterase