Determination of 3-hydroxykynurenine in human brain and plasma by high-performance liquid chromatography with electrochemical detection. Increased concentrations in hepatic encephalopathy.
A simple and specific method was developed for the determination of 3-hydroxykynurenine in brain tissue and blood plasma using high-performance liquid chromatography with electrochemical detection. This involved an extraction procedure using strong cation-exchange columns and also permitted the determination of 3-hydroxyanthranilic acid in brain tissue. The method was applied to the investigation of post mortem brain tissue from patients with hepatic encephalopathy. Cortical 3-hydroxykynurenine concentrations were substantially increased in such patients above control values, providing evidence for a dysfunction of tryptophan metabolism in this disease.
Pearson
S J
SJ
Department of Biomedical Science, University of Sheffield, U.K.
Reynolds
G P
GP
D001921:Brain / Q000378:metabolism / Q000473:pathology; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006501:Hepatic Encephalopathy / Q000097:blood / Q000473:pathology; D006801:Humans; D007737:Kynurenine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood
C005045:3-hydroxykynurenine; D007737:Kynurenine