title: Species differences in the infectability of vascular grafts.
abstract: The susceptibility of different species to bacteremia may influence the results of studies on vascular graft infection. The present study compares prosthetic graft infection in canine and porcine models. Thirty-four mongrel dogs and 38 Yorkshire pigs underwent replacement of the infrarenal aorta with a 3-cm segment of a woven Dacron prosthesis. At the time of closure, each animal received an intravenous inoculum of 10(2) to 10(8) Staphylococcus aureus (S. aureus). Graft cultures at 1 week produced a predictable infection rate in dogs, while pigs developed only random infections (dogs: 23/34; pigs: 7/38; p = .0001). The median infective dose (ID50) in dogs was 10(2.9) but pigs did not develop enough infections to determine this value. Electron microscopy revealed a smooth fibrin surface in grafts explanted from pigs, while grafts from dogs demonstrated bacteria enmeshed in an irregular fibrinous lining. Prosthetic vascular grafts in dogs are more susceptible to hematogenous infection than those in pigs. Because hematogenous infection in humans is a rare event, the swine model may be a more appropriate representation of the clinical situation.
authors.affiliation: Cardiovascular Research Laboratory, Royal Victoria Hospital-McGill University Montréal, Québec, Canada.
authors.forename: M A
authors.initials: MA
authors.lastname: Ricci
authors.forename: R J
authors.initials: RJ
authors.lastname: Mehran
authors.forename: D
authors.initials: D
authors.lastname: Petsikas
authors.forename: F
authors.initials: F
authors.lastname: Mohamed
authors.forename: R
authors.initials: R
authors.lastname: Guidoin
authors.forename: Y
authors.initials: Y
authors.lastname: Marois
authors.forename: N V
authors.initials: NV
authors.lastname: Christou
authors.forename: A
authors.initials: A
authors.lastname: Graham
authors.forename: J F
authors.initials: JF
authors.lastname: Symes
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis / Q000009:adverse effects*; D004285:Dogs; D008855:Microscopy, Electron, Scanning; D018805:Sepsis / Q000209:etiology*; D013045:Species Specificity; D013203:Staphylococcal Infections / Q000209:etiology; D013552:Swine