title: A serogroup specific enzyme-linked immunosorbent assay for the detection and identification of African horse sickness viruses.
abstract: A serogroup specific, indirect, sandwich ELISA was developed for the rapid detection of African horse sickness virus and viral antigens in field samples or in infected tissue cultures. The assay was shown to be highly sensitive and capable of providing confirmation of clinical diagnosis within one day. The results demonstrated that this ELISA will be useful for epidemiological surveillance of insect and mammalian host populations.
authors.affiliation: AFRC Institute for Animal Health, Pirbright Laboratory, Woking, U.K.
authors.forename: C
authors.initials: C
authors.lastname: Hamblin
authors.forename: P P
authors.initials: PP
authors.lastname: Mertens
authors.forename: P S
authors.initials: PS
authors.lastname: Mellor
authors.forename: J N
authors.initials: JN
authors.lastname: Burroughs
authors.forename: J R
authors.initials: JR
authors.lastname: Crowther
mesh_terms: D000355:African Horse Sickness / Q000175:diagnosis* / Q000453:epidemiology; D000356:African Horse Sickness Virus / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002478:Cells, Cultured; D006224:Cricetinae; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D006736:Horses; D012680:Sensitivity and Specificity; D013154:Spleen / Q000382:microbiology
chemical_list: D000956:Antigens, Viral