title: Isolation and characterization of a thrombin inhibitor from the tick ixodes ricinus.
abstract: The secretion of the salivary glands of ticks, Ixodes ricinus, contains anticoagulant substances. Some years ago an antithromboplastin, ixodin, was described. The present paper refers the isolation and characterization of a second anticoagulant substance of the ticks named ixin. Ixin proved to be a relatively stable and specific thrombin inhibitor. The multi step procedure for isolation results in a 850-fold purification. The preparation obtained has a specific activity of 250 antithrombin units/mg. It is not homogeneous and still contaminated. The substance was assumed to be a miniprotein.
authors.affiliation: Institute of Pharmacology and Toxicology, Medical Academy Erfurt.
authors.forename: A
authors.initials: A
authors.lastname: Hoffmann
authors.forename: P
authors.initials: P
authors.lastname: Walsmann
authors.forename: G
authors.initials: G
authors.lastname: Riesener
authors.forename: M
authors.initials: M
authors.lastname: Paintz
authors.forename: F
authors.initials: F
authors.lastname: Markwardt
mesh_terms: D000577:Amides / Q000378:metabolism; D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D010450:Endopeptidases / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D011489:Protein Denaturation; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013917:Thrombin / Q000037:antagonists & inhibitors* / Q000737:chemistry; D013987:Ticks / Q000502:physiology*
chemical_list: D000577:Amides; D011506:Proteins; D010450:Endopeptidases; D013917:Thrombin