An improved purification procedure for sulfite oxidase from bovine liver.
Sulfite oxidase (Sulfite:O2 oxidoreductase, EC 1.8.3.1) has been purified 2,440-fold from bovine liver. The procedure developed was used to isolate the enzyme from 1,000 g of liver and permitted the rapid isolation of enzyme with a very high specific activity (40,405 mU/mg). The enzyme preparations obtained have been characterized by electrophoretic and spectrophotometric analysis and the molecular mass and the Stokes radius of the enzyme have been determined.
Cabré
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Department of Biochemistry and Physiology, Faculty of Chemistry, University of Barcelona, Catalonia, Spain.
Cascante
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Canela
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D000096:Acetone; D000645:Ammonium Sulfate; D000818:Animals; D002417:Cattle; D002458:Cell Fractionation; D011232:Chemical Precipitation; D002845:Chromatography; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology*; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000302:isolation & purification*; D013057:Spectrum Analysis; D013696:Temperature
D000096:Acetone; D050862:Oxidoreductases Acting on Sulfur Group Donors; D000645:Ammonium Sulfate