[The spontaneous synaptic activity in a cell culture of chick embryo spinal cord].
Development of spontaneous synaptic activity was investigated during first 2.5 weeks in culture by means of the "hole-cell" version of the patch-clamp technique. Glycine-mediated giant postsynaptic currents (PSCs) were the most prominent property of the synaptic activity pattern. Excitatory PSCs were not observed until 14 days in culture. The role of culture conditions for the maturation of spontaneous activity has been shown. Possible mechanisms of the burst activity generation are discussed.
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D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects / Q000502:physiology; D002642:Chick Embryo; D005998:Glycine / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009433:Neural Inhibition / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010852:Picrotoxin / Q000494:pharmacology; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013331:Strychnine / Q000494:pharmacology; D013569:Synapses / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D005998:Glycine; D010852:Picrotoxin; D013331:Strychnine; D005680:gamma-Aminobutyric Acid
Spontannaia sinapticheskaia aktivnost' v kulture kletok spinnogo mozga kurinogo émbriona.