[Detection of hybridization probes using antibodies to DNA adducts with cis- and trans-diaminodichloroplatinum (II). I. Immunochemical characteristics of DNA modified by cis- and trans-diaminodichloroplatinum (II)].
Rabbit antisera elicited against calf thymus DNA modified cis- and trans-diaminedichlorplatinum II (DDP) [Pt/nucleotide ratio - 0.1] contain antibodies specific for Pt-modified DNA immunogen. A specific antibody against DNA-cisDDP and DNA-transDDP was obtained by affinity chromatography. In the ELISA, 3.10(-1 6) mol Pt per micrograms DNA was determined. The epitope structure was determined by using DNA modified with cisDDP and transDDP.
Kiseleva
V I
VI
Vrana
O
O
Brabets
I
I
Poverennyĭ
A M
AM
D000818:Animals; D002417:Cattle; D002945:Cisplatin / Q000494:pharmacology*; D004249:DNA Damage; D007150:Immunohistochemistry; D009693:Nucleic Acid Hybridization; D013237:Stereoisomerism; D013950:Thymus Gland / Q000378:metabolism
C034697:transplatin; D002945:Cisplatin
Detektsiia gibridizatsionnykh zondov s pomoshch'iu antitel k adduktam DNK s tsis- i trans-diamindikhlorplatinoĭ (II). I. Immunokhimicheskaia kharakteristika DNK, modifitsirovannoĭ tsis- i trans-diamindikhlorplatinoĭ (II).