Microbial models of mammalian metabolism: production of 3'-hydroxywarfarin, a new metabolite of warfarin using Cunninghamella elegans.
Warfarin, an anticoagulant and "metabolic probe" for cytochrome P-450 isozyme multiplicity, was metabolized by the fungus Cunninghamella elegans (ATCC 36112) to yield the previously unreported metabolite 3'-hydroxywarfarin. This metabolite was isolated from cell suspension cultures and characterized by analytical (HPLC) and spectral (EI-MS, PMR) comparisons with synthetic 3'-hydroxywarfarin.
Wong
Y W
YW
Division of Medicinal Chemistry, College of Pharmacy, University of Texas, Austin 78712.
Davis
P J
PJ
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D015394:Molecular Structure; D009090:Mucorales / Q000378:metabolism*; D014859:Warfarin / Q000031:analogs & derivatives* / Q000378:metabolism*
C070225:3'-hydroxywarfarin; D014859:Warfarin