title: The oligomeric structure of rat and human gastric mucins.
abstract: Intact oligomeric gastric mucins were isolated from the fundic part of rat and human stomach. Physicochemical properties of the oligomeric mucins from both species, such as buoyant density, molecular mass, proteinase-resistance, amino acid composition and monosaccharide composition were similar. Biochemical analysis showed that the oligomeric mucins from both species consist of disulphide-linked mucin monomers exclusively: no other covalently attached proteins were detected in purified monomeric mucin. Four major differences were found between the monomeric mucins of these species: (1) the human monomer is larger, (2) the proteolytic-digest peptides derived from proteinase-sensitive portions of the polypeptide backbone displayed no sequence similarity, (3) the human mucin was less sulphated compared with rat mucin, and (4) the proteinase-sensitive part of the human mucin was relatively larger. However, analyses of [3H]galactose-labelled mucin from both species on gel filtration revealed that both gastric mucins were exclusively synthesized as oligomers. The results indicate that the oligomeric structures of human and rat gastric mucin are similar and their biosyntheses are not affected by the differences in the subunits.
authors.affiliation: Laboratory for Cell Biology, Medical School, University of Utrecht, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Dekker
authors.forename: P H
authors.initials: PH
authors.lastname: Aelmans
authors.forename: G J
authors.initials: GJ
authors.lastname: Strous
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D005753:Gastric Mucosa / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay; D046911:Macromolecular Substances; D008970:Molecular Weight; D009077:Mucins / Q000737:chemistry* / Q000302:isolation & purification; D010446:Peptide Fragments / Q000302:isolation & purification; D051381:Rats; D013464:Sulfuric Acids / Q000032:analysis
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D046911:Macromolecular Substances; D009077:Mucins; D010446:Peptide Fragments; D013464:Sulfuric Acids