Endothelin production by amnion and its regulation by cytokines.
Cells derived from human amnion, an avascular tissue, produced significant amounts of endothelin 1 and 2, although the rate of biosynthesis was approximately one tenth of that of human umbilical vein endothelial cells. Endothelin 1 and 2 was not produced in measurable amounts by cells derived from chorion laeve or decidua. Production of endothelin 1 and 2 by amnion cells was increased by treatment with the cytokines interleukin-1 beta, tumor necrosis factor, and interleukin-6. The biosynthesis of endothelin 1 and 2 by an avascular tissue is suggestive of a wider role for endothelins than has been recognized.
Mitchell
M D
MD
Department of Obstetrics and Gynecology, University of Utah, Salt Lake City.
Lundin-Schiller
S
S
Edwin
S S
SS
D000650:Amnion / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media; D016207:Cytokines / Q000494:pharmacology*; D016232:Endothelins / Q000096:biosynthesis*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D008233:Lymphotoxin-alpha / Q000494:pharmacology; D011863:Radioimmunoassay
D003470:Culture Media; D016207:Cytokines; D016232:Endothelins; D007375:Interleukin-1; D015850:Interleukin-6; D008233:Lymphotoxin-alpha