title: Phenotypic diversity in the alpha C protein of group B streptococci.
abstract: Group B streptococci (GBS) is the leading cause of neonatal sepsis and meningitis. C proteins are an immunologically important group of surface-associated antigens in GBS that remain incompletely characterized. Two C proteins have been designated alpha and beta on the basis of protease susceptibility. We recently used a monoclonal antibody to describe a protective epitope of the GBS alpha (or trypsin-resistant) C protein in the prototype Ia/c GBS strain. In the present study, we examined 51 GBS isolates for expression of C-protein alpha and beta antigens. The alpha antigen, as detected with monoclonal antibody in sodium dodecyl sulfate (SDS) extracts, appears as a heterogeneous series of proteins spaced 8 kDa apart on SDS-polyacrylamide gel electrophoresis, but has a maximum molecular mass that varies among strains from 62.5 to 167 kDa. By immunoblotting with human immunoglobulin A, polyclonal antiserum, or monoclonal antibody, the beta antigen, in contrast, appears as a single protein of molecular mass between 124 and 134 kDa. The amount of alpha antigen expressed by each strain was quantified by enzyme immunoassay inhibition and was found to vary markedly from strain to strain. The susceptibility of strains of GBS to opsonization and killing by human polymorphonuclear leukocytes in the presence of either complement alone or complement with alpha-specific monoclonal antibody was examined. Strains expressing the alpha antigen were less readily killed in the absence of specific antibody than were alpha-negative strains. Killing in the presence of alpha-specific monoclonal antibody was found to correlate directly with the maximum molecular mass of the alpha antigen and with the quantity of antigen on the bacterial cell surface. Isolates of GBS that express the alpha C protein vary widely in the quantity and molecular mass of the alpha antigen produced, and this heterogeneity appears to have biologic importance.
authors.affiliation: Channing Laboratory, Brigham and Women's Hospital, Boston, Massachusetts.
authors.forename: L C
authors.initials: LC
authors.lastname: Madoff
authors.forename: S
authors.initials: S
authors.lastname: Hori
authors.forename: J L
authors.initials: JL
authors.lastname: Michel
authors.forename: C J
authors.initials: CJ
authors.lastname: Baker
authors.forename: D L
authors.initials: DL
authors.lastname: Kasper
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000912:Antibodies, Neoplasm / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D014644:Genetic Variation; D006801:Humans; D007106:Immune Sera; D015151:Immunoblotting; D007070:Immunoglobulin A / Q000276:immunology; D009101:Multiple Myeloma / Q000276:immunology; D010587:Phagocytosis; D010641:Phenotype; D013292:Streptococcus agalactiae / Q000235:genetics / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000942:Antigens, Bacterial; D007106:Immune Sera; D007070:Immunoglobulin A