Mismatch-stimulated plasmid integration in yeast.
A single base pair mismatch (G:T or A:C) in the CYC1 gene of the integrative plasmid pAB218 stimulates up to a five-fold integration into the yeast chromosome. Analysis of chromosomal sites of plasmid integration suggests that the mismatch-stimulated integration is not targeted as would be expected if crossovers, localised in the region of the mismatch, were a necessary step in mismatch repair. Instead, the observed mismatch-stimulated plasmid integration could be due to potentially recombinogenic structures formed during mismatch repair, such as single-stranded gaps or denatured DNA regions extending around the plasmid molecule.
Zgaga
Z
Z
Institut Curie-Biologie, Centre Universitaire, Orsay, France.
Chanet
R
R
Radman
M
M
Fabre
F
F
D001482:Base Composition; D004260:DNA Repair; D005800:Genes, Fungal; D005809:Genes, Regulator; D010957:Plasmids; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic