[Detection of strains of E. coli producing STa toxin in the feces of patients with diarrhea using a competitive ELISA system].
We evaluate the presence of heat-stable STa toxin producers E. coli strains from stools of patients with acute diarrhoea. As detection method, we used a commercially-available enzyme-linked competitive assay (ELISA). Over a three months period, 279 E. coli strains were analyzed. Only two (0.71%) were STa toxin producers. Both isolates are from pediatric cases (2 and 4 years of age), without any epidemiologic relationship between them. Therefore, we conclude that this infection could be considered as a non-epidemic community-acquired diarrhoeal disease.
Reina
J
J
Servicio de Microbiología, Hospital Son Dureta, Palma de Mallorca.
Gómez
J
J
Llompart
I
I
Borrell
N
N
Serra
A
A
D000208:Acute Disease; D000328:Adult; D001427:Bacterial Toxins / Q000032:analysis*; D002675:Child, Preschool; D003967:Diarrhea / Q000382:microbiology*; D004768:Enterotoxins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000302:isolation & purification*; D029968:Escherichia coli Proteins; D005243:Feces / Q000382:microbiology; D006801:Humans
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C034151:heat stable toxin (E coli)
Detección de cepas de E. coli productoras de toxina STa en las heces de pacientes con diarrea mediante un sistema ELISA competitivo.