title: Flow cytometric analysis of anti-platelet antibodies in patients with chronic idiopathic thrombocytopenic purpura (ITP) using acid-treated, formalin-fixed platelets.
abstract: Acid treatment of normal washed platelets prepared by albumin density gradient centrifugation followed by formalin fixation effectively eliminates HLA class I antigens from the platelet surface, without altering the immunoreactivity of platelet membrane glycoproteins Ib and IIb/IIIa complex. Using these platelets, flow cytometric analysis was performed on a total of 20 sera from different patients with chronic ITP, and revealed an inverse linear correlation between the platelet count and the mean fluorescence intensity which represents a relative amount of platelet-bound IgG. These results suggest that acid-treated, formalin-fixed platelets can be used as specimens to detect anti-platelet antibodies to differentiate from those to HLA-class I antigens.
authors.affiliation: Department of the Second Internal Medicine, Nara Medical College, Osaka University Hospital, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Mizumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Fujimura
authors.forename: K
authors.initials: K
authors.lastname: Nishikawa
authors.forename: M
authors.initials: M
authors.lastname: Uchida
authors.forename: H
authors.initials: H
authors.lastname: Fukui
authors.forename: T
authors.initials: T
authors.lastname: Morii
authors.forename: N
authors.initials: N
authors.lastname: Narita
authors.forename: Y
authors.initials: Y
authors.lastname: Kurata
mesh_terms: D000143:Acids / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000906:Antibodies / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D001792:Blood Platelets / Q000276:immunology*; D002648:Child; D005260:Female; D005434:Flow Cytometry; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010980:Platelet Membrane Glycoproteins / Q000187:drug effects; D011696:Purpura, Thrombocytopenic / Q000276:immunology*
chemical_list: D000143:Acids; D000906:Antibodies; D000954:Antigens, Surface; D006680:HLA Antigens; D010980:Platelet Membrane Glycoproteins