Determination of diclofenac in plasma using a fully automated analytical system combining liquid-solid extraction with liquid chromatography.
A fully automated analytical system based on liquid-solid extraction combined with column liquid chromatography is described for the determination of diclofenac in plasma. After addition of pH 5 buffer and the internal standard solution to the plasma sample, both sample preparation via a C18 disposable extraction column and injection were performed by a Gilson ASPEC system. Diclofenac and the internal standard were separated on a reversed-phase column, using methanol-pH 7.2 phosphate buffer (56:44, v/v) as mobile phase at a flow-rate of 0.4 ml/min. The reproducibility and accuracy of the method were acceptable over the concentration range 31-3140 nmol/l in plasma.
Sioufi
A
A
Laboratoires Ciba-Geigy, Biopharmaceutical Research Centre, Rueil-Malmaison, France.
Richard
J
J
Mangoni
P
P
Godbillon
J
J
D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004008:Diclofenac / Q000032:analysis*; D006801:Humans; D010949:Plasma / Q000737:chemistry*
D004008:Diclofenac