title: Effect of parathyroid hormone on release of interleukin 1 and interleukin 6 from cultured mouse osteoblastic cells.
abstract: This study was undertaken to examine the possibility that parathyroid hormone promotes the production of interleukin 1 and 6 in MC3T3-E1 osteoblastic cells derived from mouse calvaria. The cells were incubated in serum-free medium with or without synthetic human parathyroid hormone(1-34). The concentrations of interleukin 1 and 6 in the culture medium were determined by using specific bioassay. The cells cultured without parathyroid hormone for 24 hr released both of interleukins, and parathyroid hormone stimulated the release in a dose-dependent manner. When the cells were cultured with 10(-6) M parathyroid hormone, the release of both interleukins from the cells remained higher than control up to 144 hr. These results suggest that interleukin 1 and 6 release stimulated by parathyroid hormone may be involved in the bone resorbing activity of the hormone.
authors.affiliation: Department of Geriatrics, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: N H
authors.initials: NH
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Ouchi
authors.forename: Y
authors.initials: Y
authors.lastname: Okamoto
authors.forename: A
authors.initials: A
authors.lastname: Masuyama
authors.forename: M
authors.initials: M
authors.lastname: Kaneki
authors.forename: A
authors.initials: A
authors.lastname: Futami
authors.forename: T
authors.initials: T
authors.lastname: Hosoi
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Orimo
mesh_terms: D000818:Animals; D000906:Antibodies; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects; D007375:Interleukin-1 / Q000276:immunology / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology*; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D019379:Teriparatide; D013997:Time Factors
chemical_list: D000906:Antibodies; D007375:Interleukin-1; D015850:Interleukin-6; D010281:Parathyroid Hormone; D010446:Peptide Fragments; D019379:Teriparatide