Tension responses to rapid pressure release in glycerinated rabbit muscle fibers.
We have previously shown that the isometric tension of a fully calcium-activated skinned rabbit psoas muscle fiber is reversibly depressed by increased hydrostatic pressure. We report here the characterization of tension transients induced by a rapid (less than 1-ms) release of increased pressure at 12 degrees C. The tension transient consists of three clear phases, an initial further decrease of tension in phase with pressure change followed by two phases of tension increase back to the level recorded at ambient pressure. The mean reciprocal relaxation time for phase 2 (1/tau 2) was approximately 17 s-1 and that for phase 3 (1/tau 3) was 3 s-1. The presence of 20 mM inorganic phosphate markedly increased 1/tau 2 to approximately 52 s-1 and decreased 1/tau 3 to approximately 1.7 s-1. These observations are interpreted in terms of a pressure-sensitive transition between two attached crossbridge states of low (or zero) and higher force. This is compatible with the pressure-sensitive isomerization of actomyosin previously observed in solution. The results presented allow us to propose a coupling between a specific pressure-sensitive isomerization of purified actomyosin, the phosphate release step of the ATPase pathway, and the force-generating event of the cross-bridge cycle.
Fortune
N S
NS
Department of Biochemistry, University of Bristol, School of Medical Sciences, United Kingdom.
Geeves
M A
MA
Ranatunga
K W
KW
D000818:Animals; D006874:Hydrostatic Pressure; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000187:drug effects; D007700:Kinetics; D008954:Models, Biological; D009132:Muscles / Q000187:drug effects / Q000502:physiology*; D010710:Phosphates / Q000494:pharmacology; D011817:Rabbits; D013997:Time Factors
D010710:Phosphates