title: Relationship of VLDL receptor and LPL in metabolism of VLDL by macrophage.
abstract: Macrophages were incubated with 125I-VLDL for 5 h in presence or absence of lipoprotein lipase (LPL) inhibitor, benzene boronic acid (BBA). Both the uptake and degradation of 125I-VLDL by macrophages were saturable, and the uptake and degradation curves were virtually identical. When macrophages were incubated with 125I-VLDL for 10 h in presence of BBA, the uptake and degradation of 125I-VLDL were still saturable. However, in absence of BBA, the uptake and degradation were no longer saturable. The results suggest that with macrophages incubated with VLDL for a shorter period, VLDL was taken up predominantly via receptor pathway, with a longer period of incubation, LPL played a striking role in uptake of VLDL.
authors.affiliation: Department of Biochemistry, Tongji Medical University, Wuhan.
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Deng
authors.forename: Z C
authors.initials: ZC
authors.lastname: Feng
authors.forename: H X
authors.initials: HX
authors.lastname: Wang
authors.forename: W G
authors.initials: WG
authors.lastname: Jiang
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Zhong
authors.forename: C F
authors.initials: CF
authors.lastname: Wang
mesh_terms: D000818:Animals; D001897:Boronic Acids / Q000494:pharmacology; D002478:Cells, Cultured; D005260:Female; D008071:Lipoprotein Lipase / Q000502:physiology*; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D011973:Receptors, LDL / Q000502:physiology*; D013552:Swine
chemical_list: D001897:Boronic Acids; D008079:Lipoproteins, VLDL; D011973:Receptors, LDL; C036053:VLDL receptor; D008071:Lipoprotein Lipase; C010686:benzeneboronic acid