[Optimal chips for megabase DNA sequencing].
The SHOM method (Sequencing by Hybridization with Oligonucleotide Matrix) developed in 1988 is a new approach to nucleic acid sequencing by hybridization to a octanucleotide matrix composed of an array of immobilized oligonucleotides. The original matrix proposed for sequencing by SHOM had to contain at least 65,536 octanucleotides. The present work describes a new family of matrices for sequencing, which allows one to reduce the number of synthesized oligonucleotides 5-15 times without essentially decreasing the resolving power of the method.
Pevzner
P A
PA
Lysov
Iu P
IuP
Khrapko
K R
KR
Beliavskiĭ
A V
AV
Florent'ev
V L
VL
Mirzabekov
A D
AD
D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization
D004247:DNA
Optimal'nye chipy dlia megabaznogo sekvenirovaniia DNK.