Evidence for the involvement of acylglycerides on chitin synthetase activity in Candida albicans.
The effect of a lipase activity (EC 3.1.1.3) on the chitin synthetase from Candida albicans has been studied, both on the active and the trypsin activated enzyme. Removal of fatty acids from acylglycerides by lipase has an inhibitory effect on the activity as well as on the 'in vitro' activation process by trypsin in the membrane-bound enzyme and in the chitosomes. This would indicate that an adequate lipid environment is required for both the activation process and proper function of the synthetase activity.
Gozalbo
D
D
Department of Microbiology, Faculty of Pharmacy, University of Valencia, Spain.
Dubón
F
F
Sentandreu
R
R
D000215:Acylation; D002176:Candida albicans / Q000201:enzymology*; D002687:Chitin Synthase / Q000032:analysis*; D005989:Glycerides / Q000502:physiology*; D008049:Lipase / Q000494:pharmacology
D005989:Glycerides; D002687:Chitin Synthase; D008049:Lipase