Transport of taurine and its regulation by protein kinase C in the JAR human placental choriocarcinoma cell line.
The JAR human placental choriocarcinoma cell line transports taurine, concentrating it over 1000-fold inside the cell. The transport system is energized by a Na+ gradient and exhibits an absolute requirement for Cl-. Neutral beta-amino acids such as beta-alanine and hypotaurine effectively compete with the system, whereas neutral alpha-amino acids such as alanine, leucine and alpha-aminoisobutyric acid do not. The transport system interacts with gamma-aminobutyric acid to an appreciable extent. Kinetic analysis reveals that the taurine transport system in this cell line is of a high-affinity and low-capacity type (apparent dissociation constant 2.3 +/- 0.3 microM; maximal velocity 88.5 +/- 5.0 pmol/3 min per mg of protein). Pretreatment of the JAR choriocarcinoma cells with phorbol 12-myristate 13-acetate results in the inhibition of the taurine transport system in a dose-dependent manner. The inhibition is blocked by co-treatment of the cells with staurosporine, an inhibitor of protein kinase C. The inactive phorbol ester, 4 alpha-phorbol 12,13-didecanoate, has no effect on the transport system. These data show that the choriocarcinoma cells express a taurine transporter with characteristics similar to those of the taurine transporter described in the normal human placenta, and that the activity of the transporter in these cells is under the regulatory control of protein kinase C.
Kulanthaivel
P
P
Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta 30912-2100.
Cool
D R
DR
Ramamoorthy
S
S
Mahesh
V B
VB
Leibach
F H
FH
Ganapathy
V
V
D000470:Alkaloids / Q000494:pharmacology; D000596:Amino Acids / Q000494:pharmacology; D000838:Anions; D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D002822:Choriocarcinoma; D005260:Female; D006801:Humans; D007700:Kinetics; D010703:Phorbol Esters / Q000494:pharmacology; D010920:Placenta; D011247:Pregnancy; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012964:Sodium / Q000494:pharmacology; D019311:Staurosporine; D013379:Substrate Specificity; D013654:Taurine / Q000378:metabolism*; D014594:Uterine Neoplasms
D000470:Alkaloids; D000596:Amino Acids; D000838:Anions; D010703:Phorbol Esters; D013654:Taurine; D012964:Sodium; D011493:Protein Kinase C; D019311:Staurosporine