[The effect of hypocrellin A on the lipid bilayer of erythrocyte membrane].
In this paper, using human erythrocyte membrane, the effect of Hypocrellin A on the lipid bilayer of the membrane was studied by measuring the change of the fluidity of the membrane, the energy transfer of the fluorescent probes, the shift of the fluorescent emission peaks, and the split of band-a of Hypocrellin A. The results showed that in the presence of HA, the fluidity of erythrocyte membrane was increased, the fluorescence intensity of the probes was decreased, and the fluorescence peaks shifted blue. These phenomena took place more seriously with the increment of HA concentration. Meanwhile, the band-a of HA excitation spectra was splitted. It was suggested from all of the results that HA could significantly perturb the lipid bilayer of erythrocyte membrane, there were interactions existing between the Hypocrellin A and the membrane. The HA was mainly located in the middle range of the membrane lipid bilayer when in high concentration (mainly to the 12-16 positions of the long chain fatty acid).
Du
J
J
Institute of Biophysics, Academia Sinica, Beijing.
Cheng
L
L
D004735:Energy Transfer / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000187:drug effects*; D005456:Fluorescent Dyes; D006801:Humans; D008051:Lipid Bilayers; D008560:Membrane Fluidity / Q000187:drug effects; D010569:Perylene / Q000031:analogs & derivatives* / Q000494:pharmacology; D019800:Phenol; D011809:Quinones / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents
D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D011809:Quinones; D011838:Radiation-Sensitizing Agents; D019800:Phenol; D010569:Perylene; C048388:hypocrellin A