Pharmacological modulation of the antioxidant enzymes activities and the concentration of peroxidation products in fibroblasts stimulated with elastin peptides.
1. Elastin peptides (kappa-elastin) prepared by alcoholic potassium hydroxide degradation of insoluble elastin were shown to increase the activities of antioxidant enzymes (SOD, CAT, GSH-Px) and the lipid peroxide concentration within fibroblasts. 2. The preincubation of cells with nifedipine (calcium channel antagonist) and trifluoperazine (calmodulin antagonist) caused the decrease in the activities of studied enzymes and the concentration of TBA-reactive products in fibroblasts stimulated with kappa-elastin. 3. The preincubation with ketotifen (antiallergic drug) has no effect on the activities of SOD, CAT, GSH-Px and the lipid peroxide concentration in stimulated cells. 4. These data suggest the possibilities of pharmacological modulation of the biological effects induced by elastin-derived peptides.
Gmiński
J
J
Department of Biochemistry and Chemistry, Silesian Medical Academy, Katowice, Poland.
Weglarz
L
L
Drózdz
M
M
Goss
M
M
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D004549:Elastin / Q000378:metabolism / Q000494:pharmacology*; D004798:Enzymes / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007665:Ketotifen / Q000494:pharmacology; D008054:Lipid Peroxides / Q000378:metabolism; D051379:Mice; D009543:Nifedipine / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology*; D010545:Peroxides / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D014268:Trifluoperazine / Q000494:pharmacology
D000975:Antioxidants; D004798:Enzymes; D008054:Lipid Peroxides; D010455:Peptides; D010545:Peroxides; D014268:Trifluoperazine; D004549:Elastin; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D009543:Nifedipine; D007665:Ketotifen