High-performance affinity chromatography of a chick lectin on an adsorbent based on hydrophilic polymer gel.
A mouse monoclonal antibody (SIA4-5) which reacts with a chick 14K lectin (C14K) was covalently attached to a new support for high-performance affinity chromatography, TSKgel Tresyl-5PW, which is a preactivated, polymer-based particle. The immobilized antibody (SIA4-5-5PW) thus prepared proved to be useful in measuring not only the molecular properties of C14K but also specific interactions of C14K with SIA4-5 and hapten sugars. The C14K preparation was fractionated according to the oligomeric structure and with slight differences in affinity to SIA4-5 although the former was homogeneous in sodium dodecyl sulphate polyacrylamide gel electrophoresis. Application of the method for quantitative analytical purposes was successful.
Kakita
H
H
Central Research Laboratory, Tosoh Corporation, Yamaguchi, Japan.
Nakamura
K
K
Kato
Y
Y
Oda
Y
Y
Shimura
K
K
Kasai
K
K
D000327:Adsorption; D000818:Animals; D000911:Antibodies, Monoclonal; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002642:Chick Embryo; D002846:Chromatography, Affinity; D005782:Gels; D006241:Haptens / Q000737:chemistry; D037102:Lectins / Q000302:isolation & purification*; D011108:Polymers; D011506:Proteins / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet
D000911:Antibodies, Monoclonal; D005782:Gels; D006241:Haptens; D037102:Lectins; D011108:Polymers; D011506:Proteins