title: Technique for repeated collection of cerebrospinal fluid from cisterna magna of anesthetized strain 13 guinea pigs.
abstract: To study biochemical changes in cerebrospinal fluid (CSF), we developed a reliable technique for repeated collection of CSF in anesthetized strain 13 guinea pigs. The animal's head was mounted in a stereotaxic instrument with ventral tilt at 30 degrees, and cisternal puncture was made with an L-shaped, 23-gauge needle through the shaved skin. Clear CSF was collected in a 1-ml syringe surrounded by crushed ice. Each collection procedure lasted for 3 min, and three consecutive collections produced about 0.2 ml of CSF. Sampling was repeated at 3-hr intervals. With intravenous saline infusion (10 ml/kg.hr), a total volume of 0.6-1.0 ml of CSF was collected over 6 to 12 hr. Animals maintained a mean blood pressure, heart rate, and minute volume, with few changes during CSF sampling for the entire collection.
authors.affiliation: Department of Clinical and Experimental Physiology, United States Army Medical Research Institute of Infectious Disease, Frederick, Maryland 21702-5011.
authors.forename: C T
authors.initials: CT
authors.lastname: Liu
authors.forename: Z M
authors.initials: ZM
authors.lastname: Guo
mesh_terms: D000818:Animals; D002555:Cerebrospinal Fluid; D002946:Cisterna Magna; D006168:Guinea Pigs; D006439:Hemodynamics; D008297:Male; D013129:Spinal Puncture / Q000379:methods*