Complex regulation of TGF beta expression by retinoic acid in the vitamin A-deficient rat.
We report the results of a histochemical study, using polyclonal antipeptide antibodies to the different TGF beta isoforms, which demonstrates that retinoic acid regulates the expression of TGF beta 2 in the vitamin A-deficient rat. Basal expression of TGF beta 2 diminished under conditions of vitamin A deficiency. Treatment with retinoic acid caused a rapid and transient induction of TGF beta 2 and TGF beta 3 in the epidermis, tracheobronchial and alveolar epithelium, and intestinal mucosa. Induction of TGF beta 1 expression was also observed in the epidermis. In contrast to these epithelia, expression of the three TGF beta isoforms increased in vaginal epithelium during vitamin A deficiency, and decreased following systemic administration of retinoic acid. Our results show for the first time the widespread regulation of TGF beta expression by retinoic acid in vivo, and suggest a possible mechanism by which retinoics regulate the functions of both normal and pre-neoplastic epithelia.
Glick
A B
AB
Laboratory of Chemoprevention, National Cancer Institute, Bethesda, MD 20892.
McCune
B K
BK
Abdulkarem
N
N
Flanders
K C
KC
Lumadue
J A
JA
Smith
J M
JM
Sporn
M B
MB
D000818:Animals; D003106:Colon / Q000378:metabolism; D004817:Epidermis / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000235:genetics*; D014212:Tretinoin / Q000494:pharmacology*; D014621:Vagina / Q000378:metabolism; D014802:Vitamin A Deficiency / Q000378:metabolism*
D016212:Transforming Growth Factor beta; D014212:Tretinoin