[Measurement of urinary epidermal growth factor by radioimmunoassay and its application].
Epidermal growth factor (EGF), a polypeptide hormone originally discovered in the mouse submaxillary gland, stimulates growth in a variety of tissues in several species. This hormone has recently been identified in human urine. Herein, radioimmunoassay (RIA) for human EGF (hEGF) has been developed, using human recombinant EGF as reference standard and radioiodinated tracer and antibodies raised against hEGF. Its sensitivity was somewhat higher than that of RIAs reported formerly. This method had no cross reactivity with high molecular weight EGF. Urinary concentrations of hEGF in normal males and females were 30.2 +/- 13.0 ng/mg creatinine and 46.4 +/- 17.4 ng/mg creatinine, respectively. Urinary concentration of hEGF in patients with renal cancer was somewhat low. The patients with bladder cancer had lower urinary excretion of hEGF than normal adults (p less than 0.05). However, the role of hEGF in health and disease remains to be determined.
Fuse
H
H
Department of Urology, Faculty of Medicine, Toyama Medical & Pharmaceutical University, Japan.
Mizuno
I
I
Kurimoto
F
F
Horiuchi
J
J
Katayama
T
T
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004815:Epidermal Growth Factor / Q000652:urine*; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000652:urine; D008297:Male; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000652:urine; D011863:Radioimmunoassay; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D001749:Urinary Bladder Neoplasms / Q000652:urine
D004815:Epidermal Growth Factor