title: Proposal for a procedure for complete inactivation of the Creutzfeldt-Jakob disease agent.
abstract: We have examined complete inactivation conditions on brain homogenates from mice affected with Creutzfeldt-Jakob disease agent, and recommend for routine use a reliable procedure first treating the affected materials with 1 N NaOH for 60 min and then autoclaving at 121 degrees C for 30 min.
authors.affiliation: Department of Microbiology, School of Hygienic Sciences, Kitasato University, Kanagawa, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Taguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Tamai
authors.forename: K
authors.initials: K
authors.lastname: Uchida
authors.forename: R
authors.initials: R
authors.lastname: Kitajima
authors.forename: H
authors.initials: H
authors.lastname: Kojima
authors.forename: T
authors.initials: T
authors.lastname: Kawaguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Ohtani
authors.forename: S
authors.initials: S
authors.lastname: Miura
mesh_terms: D000818:Animals; D001921:Brain / Q000382:microbiology*; D007562:Creutzfeldt-Jakob Syndrome / Q000382:microbiology* / Q000635:transmission; D006358:Hot Temperature; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D012972:Sodium Hydroxide; D013242:Sterilization / Q000379:methods*; D013997:Time Factors
chemical_list: D012972:Sodium Hydroxide