High-performance liquid chromatographic separation of bile acid pyrenacyl esters with cyclodextrin-containing mobile phase.
The high-performance liquid chromatographic separation of bile acid pyrenacyl esters with cyclodextrin-containing mobile phase is presented. Compared with conventional methods, inclusion chromatography gives much more satisfactory separation of derivatized bile acids in a short time. The application of this method to the separation of glycine-conjugated bile acids in human bile is also described.
Shimada
K
K
Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.
Komine
Y
Y
Mitamura
K
K
D001647:Bile Acids and Salts / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003505:Cyclodextrins; D005453:Fluorescence; D005998:Glycine; D006801:Humans
D001647:Bile Acids and Salts; D003505:Cyclodextrins; D005998:Glycine