title: Chloride concentration in macula densa and cortical thick ascending limb cells.
abstract: The Cl- transport through the macula densa (MD) cells is believed to be a link in the tubuloglomerular feedback (TGF) believed to be a link in the tubuloglomerular feedback (TGF) mechanism and MD-mediated renin release. One step in this transport is probably the electroneutral and furosemide-sensitive Na(+)-K(+)-2Cl- contransport on the luminal membrane of MD cells. Another step is transport through basolateral Cl- channels. In the present study the intracellular Cl- concentration, [Cl-]i, was measured in the MD and cortical thick ascending limb (cTAL) cells, and the concentration changes elicited by blocking the Na(+)-K(+)-2Cl- cotransport with furosemide or by lowering the luminal NaCl concentration determined. We also investigated the effects of blocking the basolateral Cl- channels. A preparation consisting of a segment of the cTAL, MD cells, and the attached glomerulus was dissected from rabbit kidneys. The preparation was loaded with the Cl(-)-sensitive fluorophore SPQ, and perfused by using the isolated and perfused tubule technique. The intracellular chloride concentration was determined with a video system using digital imaging that measured the intensity of the emitted SPQ fluorescence. The T 1/2 of the leakage of SPQ was found to be (197 +/- 60) min (n = 9). With 150 mM NaCl in the lumen and bath, [Cl-]i in MD cells was 47 +/- 13 mM (n = 8) and 54 +/- 13 mM (n = 5) in cTAL cells. When furosemide (10(-4) M) was added to the luminal perfusion, the MD cell [Cl-]i was reduced to 6 +/- 2 mM. The corresponding value in cTAL cells was 5 +/- 3 mM.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Physiology and Biophysics, University of Lund, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Salomonsson
authors.forename: E
authors.initials: E
authors.lastname: Gonzalez
authors.forename: P
authors.initials: P
authors.lastname: Westerlund
authors.forename: A E
authors.initials: AE
authors.lastname: Persson
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D002712:Chlorides / Q000378:metabolism*; D005246:Feedback; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000378:metabolism; D007672:Kidney Cortex / Q000166:cytology / Q000378:metabolism; D007686:Kidney Tubules, Distal / Q000166:cytology / Q000378:metabolism*; D008138:Loop of Henle / Q000166:cytology / Q000378:metabolism*; D010477:Perfusion; D011817:Rabbits
chemical_list: D002712:Chlorides