Detection of FGF-beta mRNA in chondrosarcoma cells by a new in situ hybridization technique with synthetic oligonucleotide probes.
Fibroblast growth factor beta (FGF-beta) is a potent mitogenic and angiogenic factor produced by a large number of normal and transformed cells. In this paper we report a new application of the in situ hybridization procedure which has allowed the detection of FGF-beta mRNA in chondrosarcoma cells using 35S-labelled synthetic oligonucleotide probes.
Guthridge
M
M
Department of Biochemistry, Monash University, Clayton, Victoria, Australia.
Bertolini
J
J
Schmitt
J
J
Hearn
M T
MT
D000818:Animals; D001483:Base Sequence; D002813:Chondrosarcoma / Q000235:genetics* / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000235:genetics*; D015870:Gene Expression; D007553:Isotope Labeling; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D014407:Tumor Cells, Cultured
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2