title: Oligonucleotide purification in milligram quantities.
abstract: An inexpensive and simple procedure for the purification of synthetic oligonucleotides is described. Hands-on time is only 30 to 40 minutes and multiple samples can be prepared simultaneously. One- to two-milligram quantities can easily be handled by a single column with no further purification required for the DNA to be used in a wide variety of molecular biological uses.
authors.affiliation: Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202.
authors.forename: B
authors.initials: B
authors.lastname: Freie
authors.forename: S H
authors.initials: SH
authors.lastname: Larsen
mesh_terms: D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D009841:Oligonucleotides