High-performance liquid chromatographic assay for laudanosine in biological fluids and tissue for neurotoxic studies in rats.
A procedure for the determination of laudanosine, the central nervous system active metabolite of the neuromuscular blocking drug atracurium, in serum, cerebrospinal fluid and brain is described. The method uses a readily available internal standard, ethavrine, and a single-step protein precipitation with acetonitrile followed by high-performance liquid chromatographic separation with ultraviolet detection. Norlaudanosine, the major metabolite of laudanosine, can also be quantified. Linearity of detector response was obtained between 1 and 25 micrograms/ml or micrograms/g and the method is suitable for determining neurotoxic concentrations of laudanosine in experimental animals.
Ramzan
I
I
Pharmacy Department, University of Sydney, N.S.W., Australia.
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007546:Isoquinolines / Q000032:analysis* / Q000633:toxicity; D009417:Nerve Tissue / Q000187:drug effects; D051381:Rats
D007546:Isoquinolines; C001522:laudanosine