The anti-inflammatory activity of a low molecular weight component derived from the milk of hyperimmunized cows.
"Immune" milk has been utilized as a source of biologically active compounds for many years. In the present study, a low molecular weight fraction, isolated from the milk of dairy cows hyperimmunized with a multivalent bacterial vaccine (HIMF), has been evaluated for anti-inflammatory activity. Analysis was carried out using the rat hind-paw oedema assay. HIMF was shown to have a marked anti-inflammatory effect in this model and carrageenin-induced oedema was suppressed by up to 80% in individual experiments. The agent was active following oral, subcutaneous, intramuscular, intraperitoneal or intravenous administration. Intravenous injection was particularly effective and amounts as small as 1 mg significantly reduced the inflammatory response to carrageenin. The experiments have established that milk from hyperimmunized cows contains a highly active anti-inflammatory compound and form a basis for further studies, which will attempt to isolate and further characterize the active moiety.
Ormrod
D J
DJ
Department of Medicine, University of Auckland, New Zealand.
Miller
T E
TE
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D001428:Bacterial Vaccines / Q000276:immunology*; D002351:Carrageenan; D002417:Cattle / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D005260:Female; D007114:Immunization; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D008892:Milk / Q000737:chemistry* / Q000276:immunology; D008970:Molecular Weight; D051381:Rats; D014427:Twins
D000893:Anti-Inflammatory Agents; D001428:Bacterial Vaccines; D002351:Carrageenan