title: Net phosphate transport in phosphate-starved Candida utilis: relationships with pH and K+.
abstract: Phosphate transport was studied in phosphate-starved Candida utilis using 31P-NMR and in situ pH and K(+)-specific electrodes. A transient efflux of K+ was associated with the initial net influx of orthophosphate across the plasma membrane and decrease of both the plasma membrane pH gradient and the cytosol pH. Recovery of cytosol pH, and the plasma membrane pH gradient after phosphate uptake, was slow when extracellular K+ was depleted, but rapid when K+ was added to the suspension either with orthophosphate or after phosphate uptake. Net phosphate transport into the vacuole occurs against its concentration gradient and is associated with an increase of the tonoplast pH gradient. It is proposed that transport of orthophosphate into the vacuole is mediated by an electrical uniport and driven by the tonoplast membrane potential.
authors.affiliation: Department of Microbiology, University of Queensland, St. Lucia, Australia.
authors.forename: R M
authors.initials: RM
authors.lastname: Bourne
mesh_terms: D001692:Biological Transport; D002175:Candida / Q000378:metabolism* / Q000648:ultrastructure; D002462:Cell Membrane / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D010710:Phosphates / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*
chemical_list: D003470:Culture Media; D010710:Phosphates; D011188:Potassium