Neuropeptide Y stimulates prostacyclin production in porcine vascular endothelial cells.
We investigated the effects of neuropeptide Y on the prostacyclin production of cultured porcine aortic endothelial cells by measuring the stable metabolite of prostacyclin, 6-keto-prostaglandin F1 alpha, by radioimmunoassay. Neuropeptide Y induced dose- and time-dependent stimulation of prostacyclin production by cultured porcine aortic endothelial cells. The lowest stimulatory concentration of neuropeptide Y was 10(-8) M and maximal response, a 2.8 fold rise, was obtained with 10(-6) M. The stimulation lasted at least 24 h. The effect was associated with the stimulation of arachidonic acid release. Our data suggest that neuropeptide Y may inhibit the development of atherosclerosis by stimulating prostacyclin synthesis.
Kawamura
K
K
Burnsides Research Laboratory, University of Illinois, Urbana 61801.
Smith
T L
TL
Zhou
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Q
Kummerow
F A
FA
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis*; D000818:Animals; D001013:Aorta, Thoracic; D001095:Arachidonic Acids / Q000378:metabolism; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D009478:Neuropeptide Y / Q000494:pharmacology*; D011863:Radioimmunoassay; D013552:Swine
D001095:Arachidonic Acids; D009478:Neuropeptide Y; D015121:6-Ketoprostaglandin F1 alpha