Detection of 19-nortestosterone and its urinary metabolites in miniature pigs by gas chromatography-mass spectrometry.
The metabolism of 19-nortestosterone was investigated in a miniature non-castrated male pig (boar), in a castrated pig (barrow) and in a female pig (sow). Urine samples were taken before and at regular intervals after the injection of 100 mg of Laurabolin (nortestosterone laurate). The sample clean-up consists in preliminary solid-phase extraction, followed by high-performance liquid chromatographic purification and fractionation. Finally, gas chromatography-mass spectrometry is used to identify the 19-nortestosterone metabolites.
Debruyckere
G
G
Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, State University of Ghent, Belgium.
Van Peteghem
C
C
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004962:Estranes / Q000032:analysis / Q000652:urine; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007536:Isomerism; D008297:Male; D009277:Nandrolone / Q000652:urine*; D013552:Swine; D013556:Swine, Miniature
D004962:Estranes; C016431:estrane-3,17-diol; C036015:19-norandrosterone; D009277:Nandrolone