Simplified method for the detection of DNA hydrolysis by enteric campylobacters.
A simplified medium was developed for the detection of DNase produced by enteric campylobacters. Sensitivity and reproducibility of the test were similar to that of the improved toluidine blue DNA agar method. Logistically, the simplified DNA hydrolysis test was cheaper (5.5 times) than the earlier medium. Based on this study we recommend the routine use of the simplified medium to perform the DNase test for biotyping enteric campylobacters.
Bhadra
R K
RK
Microbiology Division, National Institute of Cholera & Enteric Diseases, Calcutta.
Pal
S C
SC
Nair
G B
GB
D002167:Campylobacter / Q000145:classification / Q000201:enzymology*; D003470:Culture Media; D004247:DNA / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis
D003470:Culture Media; D004247:DNA; D003851:Deoxyribonucleases