Clonazepam serum levels in epileptic patients determined simply and rapidly by high-performance liquid chromatography using a solid-phase extraction column.
We studied the use of high-performance liquid chromatography (HPLC), using a solid phase extraction column (Bond Elut cartridge column), for the simple, rapid and sensitive determination of serum clonazepam levels in epileptic patients. Extracted aliquots were analyzed by HPLC, using a reverse phase ODS column (mu-Bondapak C18). The analytical mean recovery of clonazepam added to the blank serum averaged 99.9%. The detection limit was as high as approximately 2 ng/ml in the serum. The reproducibilities were 2.3-8.6 CV % in the within-day assay and 6.5 CV % in the between-day assay, indicating that the analysis method was effective in the determination of clonazepam serum levels. Accordingly, we suggest that the present method, using a solid phase extraction column, may be useful for the routine monitoring of clonazepam serum levels in epileptic patients.
Furuno
K
K
Department of Hospital Pharmacy, Okayama University Medical School, Japan.
Gomita
Y
Y
Araki
Y
Y
Fukuda
T
T
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D002998:Clonazepam / Q000097:blood* / Q000627:therapeutic use; D004827:Epilepsy / Q000097:blood* / Q000188:drug therapy; D006801:Humans; D008991:Monitoring, Physiologic / Q000379:methods; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D002998:Clonazepam