title: Head group precursors modify phospholipid synthesis in Schistosoma mansoni.
abstract: The two predominant phospholipids in schistosomula of Schistosoma mansoni are phosphatidylcholine (PC) and phosphatidylethanolamine (PE) which are found in a molar ratio of 0.52 (PE/PC). The incorporation of four fatty acids (arachidonic, myristic, oleic, and palmitic) and glycerol into phospholipids of schistosomula was measured. In two different media (one containing ethanolamine, the other without), all four fatty acids were predominantly incorporated into PC with a PE/PC ratio of approximately 0.1 in a 90-min label. After a 24-h chase, PC remained the predominant labeled phospholipid but the fatty acid-labeled PE/PC ratio increased slightly, the specific activity of labeled neutral lipids decreased, and the specific activity of labeled PE increased. Glycerol was incorporated with a ratio of 0.55 in the presence of ethanolamine but only 0.19 in its absence. Schistosomula also incorporate fatty acids into phosphatidylmonomethylethanolamine (PMME) and phosphatidyldimethylethanolamine (PDME) at rates intermediate to that into PE and PC in the presence of the respective head group precursor; this incorporation was inhibited by choline. Relative to PC, oleic acid is incorporated into PE, PMME, and PDME at rates higher than for palmitic acid. These results suggest that schistosomula possess acyltransferase(s) with head group specificity and that acyl chains are transferred from neutral lipids to phospholipids over time.
authors.affiliation: Department of Medicine, Harvard Medical School, Boston, MA 02115.
authors.forename: S T
authors.initials: ST
authors.lastname: Furlong
authors.forename: J P
authors.initials: JP
authors.lastname: Caulfield
mesh_terms: D000818:Animals; D002794:Choline / Q000031:analogs & derivatives / Q000494:pharmacology; D019856:Ethanolamine; D004983:Ethanolamines / Q000378:metabolism / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis* / Q000737:chemistry; D012550:Schistosoma mansoni / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D004983:Ethanolamines; D005227:Fatty Acids; D010743:Phospholipids; D019856:Ethanolamine; D002794:Choline; D005990:Glycerol