Quantitative measurement of extra-renal renin mRNA by polymerase chain reaction.
We established a simplified method of the quantitative measurement of extra-renal renin messenger RNA using polymerase chain reaction system. We could detect the renin messenger RNA in the kidney, heart, aorta and adrenal gland from a single RNA sample obtained from a Wistar-Kyoto rat. In the kidney, heart, aorta and adrenal gland, the contents of renin messenger RNA were found to be 55.8 +/- 17.8, 0.15 +/- 0.05, 0.11 +/- 0.03 and 0.16 +/- 0.07 pg/micrograms of total RNA (n = 5, mean +/- s.d.), respectively. The present method is very useful to study the extra-renal renin-angiotensin system.
Okura
T
T
2nd Department of Internal Medicine, Ehime University School of Medicine, Japan.
Kitami
Y
Y
Iwata
T
T
Hiwada
K
K
D000311:Adrenal Glands / Q000201:enzymology; D000818:Animals; D001011:Aorta / Q000201:enzymology; D001483:Base Sequence; D007668:Kidney / Q000201:enzymology; D008297:Male; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D015345:Oligonucleotide Probes; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D011921:Rats, Inbred WKY; D012083:Renin / Q000235:genetics*
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D012083:Renin