Effect of cyclosporin-A treatment on endotoxin-induced airway hyperreactivity in vivo and in vitro in guinea-pigs.
In the present study, the role of T-lymphocytes in the development of histaminergic hyperreactivity of the airways in vivo and in vitro, after intraperitoneal administration of bacterial endotoxin (1 mg/kg) to guinea-pigs was examined. Daily oral administration of the T-lymphocyte-selective immunosuppressive drug cyclosporin A (25 mg/kg) did not prevent the endotoxin-induced airway hyperreactivity in vivo. Cyclosporin A treatment itself induced histaminergic hyperreactivity of guinea-pig trachea in vitro. In cyclosporin A-pretreated guinea-pigs, endotoxin did not further potentiate the histaminergic responsiveness of isolated tracheal spirals. It can be concluded that T-lymphocytes do not play a role in the endotoxin-induced airway hyperreactivity in vivo. Furthermore, no definite conclusion concerning the involvement of T-lymphocytes in the endotoxin-induced hyperreactivity in vitro can yet be drawn.
Van Oosterhout
A J
AJ
Department of Pharmacology, Faculty of Pharmacy, University of Utrecht, The Netherlands.
Woutersen-Van Nijnanten
F M
FM
Nijkamp
F P
FP
D000818:Animals; D001249:Asthma / Q000276:immunology; D003524:Cyclosporins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004731:Endotoxins / Q000008:administration & dosage; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D012129:Respiratory Function Tests; D012130:Respiratory Hypersensitivity / Q000139:chemically induced / Q000276:immunology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D014132:Trachea / Q000187:drug effects
D003524:Cyclosporins; D004731:Endotoxins; D006632:Histamine