Eosinophil differentiation of human umbilical cord mononuclear cells and prolonged survival of mature eosinophils by murine EL-4 thymoma cell conditioned medium.
Umbilical cord mononuclear cells, HL-60 cells, HL-60 clones selected for eosinophil differentiation, and the eosinophil leukemia cell line EoL were tested for their ability to produce eosinophil peroxidase. HL-60 clones selected for eosinophil differentiation produced eosinophil peroxidase, as judged by staining of cells for cyanide-resistant peroxidase activity; however, these cells lost their ability to produce eosinophil peroxidase in long-term culture. In contrast, eosinophil precursors from human umbilical cord blood mononuclear cells stimulated with murine EL-4 conditioned medium (EL-4 CM) were regularly induced to eosinophil protein synthesis, including eosinophil peroxidase, major basic protein, eosinophil cationic protein, and eosinophil-derived neurotoxin, as assessed by cyanide-resistant peroxidase and immunofluorescence staining. This induction by EL-4 CM is either at the level of gene transcription or mRNA stabilization, as shown by the increase of total mRNA for eosinophil peroxidase, major basic protein, and eosinophil-derived neurotoxin by Northern blot analyses. Purified peripheral blood eosinophils incubated for 4 days with EL-4 CM had increased survival over control eosinophils. Moreover, this enhanced survival was specifically blocked by antiserum to interleukin 5. Our results suggest that the effects of EL-4 CM on human umbilical cord mononuclear cells and mature eosinophils are due to the presence of interleukin 5.
Ten
R M
RM
Department of Immunology, Mayo Clinic, Rochester, MN.
Butterfield
J H
JH
Kita
H
H
Weiler
D A
DA
Fischkoff
S
S
Ishizaka
T
T
Sanderson
C J
CJ
Gleich
G J
GJ
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002454:Cell Differentiation; D002470:Cell Survival; D003470:Culture Media; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D015848:Interleukin-5 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000166:cytology; D010544:Peroxidases / Q000378:metabolism; D012260:Ribonucleases; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000276:immunology
D001798:Blood Proteins; D003470:Culture Media; D047091:Eosinophil Granule Proteins; D015848:Interleukin-5; D010544:Peroxidases; D012260:Ribonucleases