Nicotinic cholinoceptive neurons of the frontal cortex are reduced in Alzheimer's disease.
The cellular distribution of nicotinic acetylcholine receptors was studied in the frontal cortex (area 10) of 1) Alzheimer patients and compared to 2) age-matched and 3) middle-aged controls using the monoclonal antibody WF 6 and an immunoperoxidase protocol. Statistical analysis revealed significant differences between the number of labeled neurons among all three groups tested (middle-aged controls greater than aged controls greater than Alzheimer cases). No differences were seen for cresyl violet-stained samples. These findings underline that the nicotinic receptor decrease found with radioligand binding may reflect a postsynaptic in addition to a presynaptic component.
Schröder
H
H
Department of Pharmacology, Southern Illinois University School of Medicine, Springfield.
Giacobini
E
E
Struble
R G
RG
Zilles
K
K
Maelicke
A
A
D000368:Aged; D000375:Aging / Q000378:metabolism; D000544:Alzheimer Disease / Q000378:metabolism*; D000911:Antibodies, Monoclonal; D048588:Benzoxazines; D005260:Female; D005625:Frontal Lobe / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D009474:Neurons / Q000378:metabolism*; D010078:Oxazines; D011978:Receptors, Nicotinic / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D048588:Benzoxazines; D010078:Oxazines; D011978:Receptors, Nicotinic; C028911:cresyl violet