Expression of HLA-DQ antigens in the small-intestinal mucosa of patients with coeliac disease.
Studies at the DNA and product level of B-cell lines of coeliac patients have shown a strong association between coeliac disease and the HLA-DQ alpha 2.3 and HLA-DQ beta 2.7 alleles. The monoclonal antisera SFR20-DQ alpha 5 and XIII-358.4, which specifically react with HLA-DQ alpha 2.3 and with HLA-DQ beta 2.3 and -DQ beta 2.7, respectively, have been used to detect the expression of these specificities in the small-intestinal mucosa of 7 coeliac patients and 11 non-coeliac persons. An immunoperoxidase technique on frozen tissue sections of jejunal biopsy specimens was used. Positive specimens showed immunoperoxidase staining of lymphocytes and histiocytes in the lamina propria. The epithelial cells showed no immunoperoxidase staining. Positive results at the intestinal level correlated with the HLA typing of the patients and controls. The distribution found for the HLA-DQ alleles in the intestinal mucosa makes the role of a HLA-DQ alpha/beta dimer as gliadin receptor at the epithelial cell less probable, but it is compatible with the hypothesis that these DQ molecules are involved in the regulation of the intestinal immune response to gluten.
Schweizer
J J
JJ
Dept. of Pediatrics, University Hospital, Leiden, The Netherlands.
Mearin
M L
ML
Peña
A S
AS
Offerhaus
G J
GJ
Dreef
E J
EJ
Roep
B O
BO
Bontrop
R E
RE
Dooren
L J
LJ
Lamers
C B
CB
Hoedemaeker
P J
PJ
D000328:Adult; D000483:Alleles; D000911:Antibodies, Monoclonal; D001706:Biopsy; D002446:Celiac Disease / Q000276:immunology*; D002675:Child, Preschool; D005260:Female; D006683:HLA-DQ Antigens / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000276:immunology*; D007583:Jejunum / Q000276:immunology*; D008297:Male
D000911:Antibodies, Monoclonal; D006683:HLA-DQ Antigens