Rabbit neuromedin U-25: lack of conservation of a posttranslational processing site.
The rabbit small intestine contains neuromedin U-like immunoreactivity (22 pmol/g wet tissue weight) that was resolved into a single major molecular form by reversed-phase HPLC. The primary structure of the peptide was established as: Phe-Pro-Val-Asp-Glu-Glu-Phe-Gln-Ser-Pro10-Phe-Gly-Ser-Arg-Ser-Arg- Gly-Tyr-Phe- Leu20-Phe-Arg-Pro-Arg-Asn.NH2. In rabbit neuromedin U, the Arg16-Arg17 dibasic residue processing site that is found in pig and dog neuromedin U-25 is replaced by Arg-Gly, but this potential monobasic processing site is not utilized by cleavage enzyme(s) in the intestine.
Kage
R
R
Clinical Research Group for Gastrointestinal Endocrinology of the Max-Planck-Gesellschaft, University of Gottingen, F.R.G.
O'Harte
F
F
Thim
L
L
Conlon
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004064:Digestive System / Q000737:chemistry; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000302:isolation & purification* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011817:Rabbits
D009479:Neuropeptides; C046271:neuromedin U 25