CD9 antigen on acute non-lymphoid leukemia cells: preferential expression by promyelocytic (M3) subtype.
A monoclonal antibody (S17-12), previously described to recognize subsets of myelomonocytic cells, is demonstrated to bind CD9 antigen, a surface marker of B-cell precursors, platelets and several non-hematopoietic tissues. The proportion of S17-12, CD9-positive leukemic cells was determined in 102 patients with acute non-lymphocytic leukemia. It was found to be above 75% in 14/22 M3 cases (including 3/3 of microgranular variant) and only in 1/80 cases of different FAB subtype. Complete reactivity (greater than 95%) of leukemic clonogenic cells with CD9 MoAB was restricted to 4/18 M3 cases. Therefore, a high proportion of CD9-positive cells seems to be peculiar to M3 cases. This may help in the diagnosis of cases that lack typical morphological features.
Ferrero
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D
Cattedra di Ematologia, Universita' di Torino, Italy.
Carlesso
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N
Gallo
E
E
Pregno
P
P
De Fabritiis
P
P
Petti
M C
MC
Mandelli
F
F
D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000378:metabolism / Q000502:physiology*; D001667:Binding, Competitive; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000276:immunology* / Q000473:pathology; D015473:Leukemia, Promyelocytic, Acute / Q000276:immunology* / Q000473:pathology; D008562:Membrane Glycoproteins; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000473:pathology; D060245:Tetraspanin 29; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; C554869:CD9 protein, human; D008562:Membrane Glycoproteins; D060245:Tetraspanin 29