title: Nucleotide sequence and characterization of the gene for secreted alkaline phosphatase from Lysobacter enzymogenes.
abstract: Lysobacter enzymogenes produces an alkaline phosphatase which is secreted into the medium. The gene for the enzyme (phoA) was isolated from a recombinant lambda library. It was identified within a 4.4-kb EcoRI-BamH1 fragment, and its sequence was determined by the chain termination method. The structural gene consists of an open reading frame which encodes a 539-amino-acid protein with a 29-residue signal sequence, followed by a 119-residue propeptide, the 281-residue mature phosphatase, and a 110-residue carboxy-terminal domain. The roles of the propeptide and the carboxy-terminal peptide remain to be determined. A molecular weight of 30,000 was determined for the mature enzyme from sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The amino acid sequence was compared with sequences available in the current protein data base, and a region of the sequence was found to show considerable homology with sequences in mammalian type 5 iron-containing purple acid phosphatases.
authors.affiliation: Department of Microbiology, University of Alberta, Edmonton, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Au
authors.forename: K L
authors.initials: KL
authors.lastname: Roy
authors.forename: R G
authors.initials: RG
authors.lastname: von Tigerstrom
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001482:Base Composition; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D003062:Codon; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D003062:Codon; D000469:Alkaline Phosphatase