[Identification of mucin-secretion characteristics in cells from serous effusions: consideration of histochemical and immunohistochemical aspects].
Fourty-two cell-blocks of serous effusions (thirty malignant and twelve reactive) were tested by anti Secretory Component Ab (policlonal) using the s.-Ab method, and by histochemical tests: PAS, PAS Diastase and Alcian blue pH 2.5. Immunohistochemical and histochemical features were compared to identify their meaning and correlation in epithelial mucin detection. This study suggests the evident correlation between SC and PAS diastase (better than between SC and Alcian blue) and possibility to replace the old traditional test of PAS diastase with anti SC antibody (much more sensible than PAS diastase) in routine mucin detection histochemical tests.
Morea
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F
Servizio di anatomia patologica, U.S.L. Bari.
L'Abbate
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A
Albrizio
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M
D000423:Alcian Blue; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D009077:Mucins / Q000032:analysis*; D010503:Periodic Acid-Schiff Reaction; D016066:Pleural Effusion, Malignant / Q000473:pathology*; D012635:Secretory Component / Q000032:analysis
D009077:Mucins; D012635:Secretory Component; D000423:Alcian Blue
Identificazione di caratteristiche mucosecernenti in cellule da versamenti sierosi: considerazioni su aspetti istochimici ed immunoistochimici.