title: Temperature recording from thermocyclers used for PCR.
abstract: Using a simple electronic circuit, a thermocouple can be connected to a chart recorder to measure the actual temperature inside a PCR tube. This allows accurate inspection of the thermocycle program and comparison between thermoprofiles from different thermocyclers. We found that the recording of temperature cycling enabled us to obtain more reliable and reproducible results.
authors.affiliation: Mt. Sinai School of Medicine, Fishberg Research Center for Neurobiology, New York, NY 10029.
authors.forename: S
authors.initials: S
authors.lastname: Stamm
authors.forename: B
authors.initials: B
authors.lastname: Gillo
authors.forename: J
authors.initials: J
authors.lastname: Brosius
mesh_terms: D001483:Base Sequence; D004247:DNA; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D015203:Reproducibility of Results; D013696:Temperature; D013821:Thermometers
chemical_list: D009841:Oligonucleotides; D004247:DNA