The CpG island in the 5' region of the G6PD gene of man and mouse.
The nucleotide (nt) sequence of the entire CpG island in the 5' region of the human glucose-6-phosphate dehydrogenase-encoding gene (G6PD) and of the corresponding region in mouse was determined. In comparison to the human gene, the 5' region of the mouse G6PD gene has highly reduced G + C and CpG dinucleotide content, but maintains the functional features of a CpG island, as it is differentially methylated on the active vs. the inactive X chromosome. In addition to the expected conservation of exons, nt sequence comparison showed that several boxes are highly conserved between the two species in the 5' flanking DNA and in the first intron. Moreover, the conservation of the position of most CpG dinucleotides in the promoter region and in one of the upstream boxes, at about -900, gives support to the hypothesis that, in each island, specific CpGs play a major role in the regulation of gene expression.
Toniolo
D
D
Istituto di Genetica Biochemica ed Evoluzionistica, CNR, 27100 Pavia, Italy.
Filippi
M
M
Dono
R
R
Lettieri
T
T
Martini
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000378:metabolism; D015226:Dinucleoside Phosphates / Q000235:genetics*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D006801:Humans; D008297:Male; D008745:Methylation; D051379:Mice; D008969:Molecular Sequence Data; D011544:Pseudogenes; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D004247:DNA; D015226:Dinucleoside Phosphates; D005954:Glucosephosphate Dehydrogenase; C015772:CpG dinucleotide