title: Photochemical cis-trans isomerization of furylacryloylpeptides and their different kinetic behavior as substrates for carboxypeptidase Y.
abstract: Furylacryloyl substrates used in kinetic measurements of proteolytic enzymes are shown to cis-trans photoisomerize quickly in plain daylight. The enzymatic transformation of the two forms is sufficiently different to cause problems for such measurements without careful protection against light.
authors.affiliation: Research Center for Medical Biotechnology, H.C. Orsted Institute, Copenhagen, Denmark.
authors.forename: A
authors.initials: A
authors.lastname: Kanstrup
authors.forename: O
authors.initials: O
authors.lastname: Buchardt
mesh_terms: D000179:Acrylates / Q000378:metabolism*; D002268:Carboxypeptidases / Q000378:metabolism*; D004151:Dipeptides / Q000378:metabolism*; D005998:Glycine / Q000031:analogs & derivatives* / Q000378:metabolism; D007700:Kinetics; D010777:Photochemistry; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D000179:Acrylates; D004151:Dipeptides; C070198:3-(2-furylacryloyl)phenylalanylleucine; C070200:3-(2-furylacryloyl)glycine; D002268:Carboxypeptidases; C022633:serine carboxypeptidase; D005998:Glycine