The mature MgPa-adhesin of Mycoplasma genitalium.
A high molecular weight protein of Mycoplasma genitalium (MgPa-protein) was isolated by fractionated solubilization with 1% CHAPS, followed by subsequent extraction with 2% octylglucoside and size exclusion chromatography. The comparison of the N-terminal sequence reported here with published nucleotide sequence data revealed the existence of a signal sequence; the molecular weight of the mature MgPa-protein was calculated to be 153, 134 dalton. The protein shares antigenic determinants with the adhesin of Mycoplasma pneumoniae (P1-protein). Therefore the amino acid sequence of the MgPa-protein was matched to the P1-protein sequence. Five of seven computer predicted hydrophobic regions of both amino acid sequences were located in corresponding regions.
Mader
B
B
Institute for Medical Microbiology and Hygiene, University of Freiburg, Germany.
Hu
P C
PC
Huang
C H
CH
Schilz
E
E
Jacobs
E
E
D018829:Adhesins, Bacterial; D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D015153:Blotting, Western; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009174:Mycoplasma / Q000032:analysis*
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; C035471:adhesin, Mycoplasma pneumoniae