Separation of translationally active mRNAs by reversed-phase ion-pair high-performance liquid chromatography.
An ion-pair high-performance liquid chromatographic method on C4 columns was developed for the separation of mRNAs. The addition of methylmercuric hydroxide markedly influenced the separation according to length of these molecules. A method is given to recover minute amounts of translatable mRNA from the organic phase. The resolution of mRNAs improved with increasing pore size of the column support.
van der Mast
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CA
Department of Molecular Cell Biology, University of Utrecht, The Netherlands.
Hekstra
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Voorma
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HO
D000818:Animals; D002474:Cell-Free System; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004587:Electrophoresis, Agar Gel; D008099:Liver / Q000737:chemistry*; D008767:Methylmercury Compounds; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats
D008767:Methylmercury Compounds; D012333:RNA, Messenger; C020771:methylmercury hydroxide