title: A natural specific intracellular ligand as a reagent for cyclosporine blood level determination.
abstract: We isolated and characterized a non-immunoglobulin fraction from calf thymuses which was found very active in binding [3H]labelled cyclosporine (CsA). It contains the natural, intracellular receptor cyclophilin, specifically binding to the active site of the CsA molecule, which may also play a part in the immunosuppression of human immunocompetent cells. This material with a high selectivity could be used, instead of specific monoclonal or polyclonal antibodies, to determine CsA levels in patient's blood.
authors.affiliation: Institute for Clinical and Experimental Medicine, Praha.
authors.forename: V
authors.initials: V
authors.lastname: Hasková
authors.forename: L
authors.initials: L
authors.lastname: Rozprimová
authors.forename: J
authors.initials: J
authors.lastname: Svobodová
mesh_terms: D000591:Amino Acid Isomerases / Q000378:metabolism*; D001665:Binding Sites; D002352:Carrier Proteins / Q000378:metabolism*; D003524:Cyclosporins / Q000097:blood* / Q000378:metabolism; D006801:Humans; D019696:Peptidylprolyl Isomerase; D013950:Thymus Gland / Q000737:chemistry*; D014462:Ultrafiltration
chemical_list: D002352:Carrier Proteins; D003524:Cyclosporins; D000591:Amino Acid Isomerases; D019696:Peptidylprolyl Isomerase