Evidence for a functional link between profilin and CAP in the yeast S. cerevisiae.
CAP is a component of the S. cerevisiae adenylyl cyclase complex. The N-terminal domain is required for cellular RAS responsiveness. Loss of the C-terminal domain is associated with morphological and nutritional defects. Here we report that cap- cells bud randomly and are defective in actin distribution. The morphological and nutritional defects associated with loss of the CAP C-terminal domain are suppressed by over-expression of PFY, the gene encoding profilin, an actin- and polyphosphoinositide-binding protein. The phenotype of cells lacking PFY resembles that of cells lacking the CAP C-terminal domain. Study of mutated yeast profilins and profilins from Acanthamoeba suggests that the ability of profilin to suppress cap- cells is dependent upon a property other than, or in addition to, its ability to bind actin. This property may be its ability to bind polyphosphoinositides. We propose that CAP and profilin provide a link between growth signals and remodeling of the cellular cytoskeleton.
Vojtek
A
A
Cold Spring Harbor Laboratory, New York 11724-2208.
Haarer
B
B
Field
J
J
Gerst
J
J
Pollard
T D
TD
Brown
S
S
Wigler
M
M
D000199:Actins / Q000502:physiology; D000262:Adenylyl Cyclases / Q000502:physiology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002455:Cell Division; D003001:Cloning, Molecular; D003285:Contractile Proteins; D003599:Cytoskeleton / Q000502:physiology; D004252:DNA Mutational Analysis; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000378:metabolism*; D005800:Genes, Fungal; D008840:Microfilament Proteins / Q000502:physiology*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D051304:Profilins; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000502:physiology*
D000199:Actins; D003285:Contractile Proteins; D004271:DNA, Fungal; D005656:Fungal Proteins; D008840:Microfilament Proteins; D009841:Oligonucleotides; D051304:Profilins; D000262:Adenylyl Cyclases