Effect of anaerobic cecal microflora and dietary lactose on colonization resistance of layer chicks to invasive Salmonella enteritidis.
The effect of oral inoculation with anaerobic cultures of cecal microflora and providing lactose in the feed on colonization resistance to invasive Salmonella enteritidis was evaluated in newly hatched leghorn chicks. Salmonella colonization of the ceca, tissue invasion and organ colonization, horizontal transmission, and seroconversion were significantly decreased (P less than 0.01) in chicks inoculated with cecal flora. The addition of lactose to the feed, in the absence of cecal microflora, failed to provide protection. Dietary lactose enhanced colonization resistance in chicks that were inoculated with anaerobic cultures of cecal flora. The results indicated that establishment of normal cecal flora in layer chicks together with the addition of lactose to the diet markedly increases resistance to cecal colonization and organ invasion, and decreases horizontal transmission of S. enteritidis.
Corrier
D E
DE
U.S. Department of Agriculture, Food Animal Protection Research Laboratory, College Station, Texas 77840.
Hargis
B
B
Hinton
A
A
Lindsey
D
D
Caldwell
D
D
Manning
J
J
DeLoach
J
J
D000821:Animal Feed; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001421:Bacteria, Anaerobic / Q000254:growth & development*; D002432:Cecum / Q000382:microbiology; D002645:Chickens; D004040:Dietary Carbohydrates / Q000494:pharmacology; D007785:Lactose / Q000494:pharmacology*; D008297:Male; D011201:Poultry Diseases / Q000276:immunology / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000382:microbiology; D012477:Salmonella enteritidis / Q000254:growth & development / Q000276:immunology*
D000907:Antibodies, Bacterial; D004040:Dietary Carbohydrates; D007785:Lactose