Simultaneous determination of testosterone and androstadienone (sex attractant) in human plasma by gas chromatography-mass spectrometry with high-resolution selected-ion monitoring.
Androsta-4,16-dien-3-one (androstadienone) and androst-4-en-3-one-17 beta-ol (testosterone) in healthy human plasma were simultaneously determined under several experimental conditions by gas chromatography-mass spectrometry with high-resolution selected-ion monitoring. Internal standards were [2,2,4,6,6-2H5]androstadienone and [2,2,4,6,6-2H5]testosterone. Samples were extracted with an Extrelut column, purified using Lipidex 5000 and converted into hydroxime-trimethylsilyl derivatives for determination. Physiological concentrations of androstadienone and testosterone found in eleven healthy men were 2.05 +/- 0.74 and 18.6 +/- 4.9 pmol/ml in plasma (mean +/- S.D.), respectively. No correlation was observed between these steroid concentrations.
Fukushima
S
S
Department of Legal Medicine, Shimane Medical University, Izumo, Japan.
Akane
A
A
Matsubara
K
K
Shiono
H
H
Morishita
H
H
Nakada
F
F
D000328:Adult; D000730:Androstadienes / Q000097:blood*; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D006801:Humans; D008297:Male; D013739:Testosterone / Q000097:blood*
D000730:Androstadienes; D013739:Testosterone; C003524:4,16-androstadien-3-one; D003903:Deuterium