Interactions between chromosomal omnipotent suppressors and extrachromosomal effectors in Saccharomyces cerevisiae.
Chromosomal omnipotent suppressor mutations recovered in psi+ strains of Saccharomyces cerevisiae were brought into psi- cytoplasm. SUP46, SUP138 and SUP139 acted as dominant omnipotent suppressors in the psi- cytoplasm though their suppressor activity was substantially reduced. SUP46 and SUP138 conferred recessive thermosensitivity and antibiotic sensitivity in psi- cytoplasm as in psi+ cytoplasm. On the other hand, sup111 through sup115, which acted as recessive omnipotent suppressors in the psi+ cytoplasm, manifested no, or very low, suppressor activity in the psi- cytoplasm. They, however, still enhanced the efficiency of the SUP29 tRNA suppressor in psi- cytoplasm. A multicopy plasmid carrying the wild-type SUP35 gene enhanced the efficiency of sup111 in psi- cytoplasm.
Ono
B
B
Laboratory of Environmental Hygiene Chemistry, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Chernoff
Y O
YO
Ishino-Arao
Y
Y
Yamagishi
N
N
Shinoda
S
S
Inge-Vechtomov
S G
SG
D005111:Extrachromosomal Inheritance; D005799:Genes, Dominant; D005800:Genes, Fungal; D005808:Genes, Recessive; D016153:Genes, Suppressor; D005816:Genetic Complementation Test; D010957:Plasmids; D014176:Protein Biosynthesis; D012331:RNA, Fungal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D012331:RNA, Fungal; D012343:RNA, Transfer