title: Histiocytic neoplasias: immunohistochemical evaluation of their frequencies among malignant lymphoma and related conditions in Japan.
abstract: Through histologic review of 1,766 cases with malignant lymphoma and related conditions, 35 cases (2%) were selected as probable histiocytic neoplasias. Proliferating cells in these cases had voluminous, granulated cytoplasm, and round to irregularly shaped nuclei often with bi- or multinucleated forms showing monomorphous or polymorphous proliferation accompanying small lymphocytes, plasma cells, and, less frequently, eosinophils. Cases showing proliferation of convoluted cells with numerous benign-appearing histiocytes or large cells with clear cytoplasm were excluded under a diagnosis of T-cell lymphoma. To evaluate the immunologic character of proliferating cells, immunohistochemistry using antibodies Mx-Pan B, MB-1, MT-1, UCHL-1, lysozyme, alpha 1-antitrypsin, alpha 1-antichymotrypsin, S-100 alpha, S-100 beta, Leu M1, epithelial membrane antigen, and Ki-1 were carried out in 23 cases. Naphthol-ASD-chloracetate reaction and toluidine blue stain were also performed. These procedures revealed that 12 cases (52%) were B-cell type, three cases (13%) T-cell type, six cases (26%) true histiocytic type, and two cases null type. Therefore, the frequency of cases with true histiocytic neoplasias among cases with malignant lymphoma and related conditions in Japan may be 0.5%.
authors.affiliation: Department of Pathology, Nara Medical University, Kashihara, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Aozasa
authors.forename: M
authors.initials: M
authors.lastname: Ohsawa
authors.forename: K
authors.initials: K
authors.lastname: Saeki
authors.forename: K
authors.initials: K
authors.lastname: Horiuchi
authors.forename: M
authors.initials: M
authors.lastname: Tamai
authors.forename: T
authors.initials: T
authors.lastname: Kohro
authors.forename: A
authors.initials: A
authors.lastname: Kurata
mesh_terms: D000911:Antibodies, Monoclonal; D005260:Female; D006644:Histiocytes / Q000648:ultrastructure; D054747:Histiocytic Sarcoma / Q000453:epidemiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007564:Japan / Q000453:epidemiology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000737:chemistry / Q000453:epidemiology*; D008297:Male; D008875:Middle Aged
chemical_list: D000911:Antibodies, Monoclonal