title: [Ultrasonically nebulized hypertonic saline as a provocation agent in asthmatics].
abstract: We investigated nonspecific reactivity to inhaled aerosol of hypertonic saline. Hyperosmotic challenge carried out with NaCl solution of 0.9-fold increasing concentration from 2.7 to 7.2%. Each aerosol inhalation at different concentration was carried out for 5 min. An ultrasonic nebulizer was used to generate aerosol of NaCl solution that was delivered by tubing to a two-way valve. FEV1 was measured 1.0, 1.3 and 2 min after each concentration and did further at 5 min intervals until the value reached a plateau or began to rise. The challenge was terminated when the percentage fall in FEV1 was greater than 20%. The response to HS was expressed as PC20. In the 26 subjects with stable asthma 23 were responsive to HS. The PC20-HS was from 1.65 to 6.06%. The peak bronchoconstrictor effect of HS inhalation occurred 3 min after inhalation and its mean duration was 45 min. We also compared bronchial response to HS with that to methacholine (MCH). There was a significant correlation between PC20-HS and PC20-MCH (r = 0.538, P less than 0.01). The method used for HS challenge is simple, rapid, inexpensive and provide a new means for determination of bronchial hyperreactivity.
authors.affiliation: Department of Respiratory Disease, XiJing Hospital, China.
authors.forename: B
authors.initials: B
authors.lastname: Sun
mesh_terms: D000293:Adolescent; D000328:Adult; D001249:Asthma / Q000175:diagnosis* / Q000503:physiopathology; D001985:Bronchial Provocation Tests / Q000379:methods*; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D008297:Male; D008875:Middle Aged; D009330:Nebulizers and Vaporizers; D012462:Saline Solution, Hypertonic
chemical_list: D012462:Saline Solution, Hypertonic