Glycoprotein patterns in normal and malignant cervical tissue.
Glycoproteins from normal and malignant human cervix were studied using an organ culture system and compared by gel electrophoresis and autoradiography. Five glycoproteins of 178 kDa, 95 kDa, 93 kDa, 82 kDa and 38 kDa and 1 glycolipid (46 kDa) were detected more frequently in squamous carcinomas. Certain glycoproteins were shown to be oncofoetal and some had affinity for Concanavalin A (Con A). The 82 kDa glycoprotein was present in 16/17 squamous carcinomas but in only 1/13 normal cervices. This band represented a glycoprotein containing glucosamine, mannose, small quantities of methionine and no fucose. These preliminary results suggest that these glycoproteins and in particular the 82-kDa glycoprotein are worthy of further investigation and characterisation.
O'Brien
M E
ME
Cancer Research Campaign Clinical Trials Unit, Selly Oak Hospital, Birmingham, U.K.
Souberbielle
B E
BE
Cowan
M E
ME
Allen
C A
CA
Luesley
D M
DM
Mould
J J
JJ
Blackledge
G R
GR
Skinner
G R
GR
D000230:Adenocarcinoma / Q000378:metabolism; D000328:Adult; D000368:Aged; D001706:Biopsy; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D002845:Chromatography; D003208:Concanavalin A; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000302:isolation & purification; D008875:Middle Aged; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*
D006023:Glycoproteins; D037102:Lectins; D003208:Concanavalin A