title: Identification and initial characterization of the eutF locus of Salmonella typhimurium.
abstract: We report the isolation and initial characterization of mutations in the newly described eutF locus of Salmonella typhimurium LT2. Mutations in eutF render a strain unable to utilize ethanolamine as a source of carbon and/or energy and impair growth on ethanolamine as a sole nitrogen source. Strains carrying eutF mutations exhibit a 2-order-of-magnitude decrease in transcription of the unlinked eutDEABCR operon (50 min), which codes for the enzymes needed to catabolize ethanolamine; have only 10% of the ethanolamine ammonia-lyase activity found in the wild type; and show a marked reduction in the rate of ethanolamine uptake. Deletion mapping and three-factor cross analysis results are consistent with the gene order cobA trp eutF tonB at 34 min on the linkage map. We discuss two possible roles for the EutF protein: (i) as an ethanolamine permease or (ii) as a transcription factor required for the expression of the eutDEABCR operon.
authors.affiliation: Department of Bacteriology, University of Wisconsin, Madison 53706.
authors.forename: G A
authors.initials: GA
authors.lastname: O'Toole
authors.forename: J C
authors.initials: JC
authors.lastname: Escalante-Semerena
mesh_terms: D002874:Chromosome Mapping; D003433:Crosses, Genetic; D019856:Ethanolamine; D004981:Ethanolamine Ammonia-Lyase / Q000378:metabolism*; D004983:Ethanolamines / Q000378:metabolism*; D007700:Kinetics; D026901:Membrane Transport Proteins / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D009876:Operon / Q000235:genetics; D010641:Phenotype; D012486:Salmonella typhimurium / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D004983:Ethanolamines; D026901:Membrane Transport Proteins; D014157:Transcription Factors; D019856:Ethanolamine; D004981:Ethanolamine Ammonia-Lyase