[Supermolecular organization of aminotransferases and dehydrogenases of alpha-ketoacid from rat brain mitochondria].
A fraction of coarse mitochondria from the rat brain was deposited after short-term effect of supersound to obtain "metabolones". Activity of dehydrogenases of alpha-ketoacids, succinate dehydrogenase, aspartate-, alanine- and GABA-alpha-ketoglutarate amino-transferases has been determined in the supernatant liquid and in "metabolones". It is shown that dehydrogenase activity is mainly (93-100%) localized in "metabolones", while the level of aminotransferase activity in the latter is lower (72-94%). Nonproportionally high activity of aminotransferases in the supernatant liquid is found to considerably suprpass a decrease in activity of these enzymes in "metabolones" against a background of extremely scanty losses of protein (within 5%) induced by the supersound effect. A hypothetic model of a "metabolone" containing the enzymes of the Krebbs cycle and GABA-shunt is suggested.
Rozanov
V A
VA
Fan Ban T'i
Gerasimiak
G R
GR
Liubarev
A E
AE
Kurganov
B I
BI
Rozanov
A Ia
AIa
D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D000612:4-Aminobutyrate Transaminase / Q000737:chemistry; D000410:Alanine Transaminase / Q000737:chemistry; D000818:Animals; D001219:Aspartate Aminotransferases / Q000737:chemistry; D001921:Brain / Q000201:enzymology*; D007655:Ketoglutarate Dehydrogenase Complex / Q000737:chemistry; D007658:Ketone Oxidoreductases / Q000737:chemistry*; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000737:chemistry*; D011487:Protein Conformation; D011768:Pyruvate Dehydrogenase Complex / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains; D013385:Succinate Dehydrogenase / Q000737:chemistry; D000637:Transaminases / Q000737:chemistry*
D009097:Multienzyme Complexes; D011768:Pyruvate Dehydrogenase Complex; D007658:Ketone Oxidoreductases; D007655:Ketoglutarate Dehydrogenase Complex; D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D013385:Succinate Dehydrogenase; D000637:Transaminases; D001219:Aspartate Aminotransferases; D000612:4-Aminobutyrate Transaminase; D000410:Alanine Transaminase
Hadmolekuliarnaia organizatsiia aminotransferaz i degidrogenaz alpha- ketokislot mitochondriĭ golovnogo mozga krys.