title: Mismatch-stimulated plasmid integration in yeast.
abstract: A single base pair mismatch (G:T or A:C) in the CYC1 gene of the integrative plasmid pAB218 stimulates up to a five-fold integration into the yeast chromosome. Analysis of chromosomal sites of plasmid integration suggests that the mismatch-stimulated integration is not targeted as would be expected if crossovers, localised in the region of the mismatch, were a necessary step in mismatch repair. Instead, the observed mismatch-stimulated plasmid integration could be due to potentially recombinogenic structures formed during mismatch repair, such as single-stranded gaps or denatured DNA regions extending around the plasmid molecule.
authors.affiliation: Institut Curie-Biologie, Centre Universitaire, Orsay, France.
authors.forename: Z
authors.initials: Z
authors.lastname: Zgaga
authors.forename: R
authors.initials: R
authors.lastname: Chanet
authors.forename: M
authors.initials: M
authors.lastname: Radman
authors.forename: F
authors.initials: F
authors.lastname: Fabre
mesh_terms: D001482:Base Composition; D004260:DNA Repair; D005800:Genes, Fungal; D005809:Genes, Regulator; D010957:Plasmids; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic