[Identification of alpha-tropomyosin of the human myocardium by two-dimensional electrophoresis, and sequencing].
Two-dimensional electrophoresis of total cardiac muscle extracts allows the detection of about 200 protein fractions. In preliminary studies the fraction D-10 protein was characterized in terms of relative molecular mass, isoelectric point and quantitative composition as alpha-tropomyosin. The similarity of the protein to human alpha-tropomyosin was confirmed by the results of analysis of the N-terminal sequence of the D-10 protein eluate in a gas-phase sequencer.
Shishkin
S S
SS
Egorov
Ts A
TsA
Kovalev
L I
LI
Laptev
A V
AV
Tsvetkova
M N
MN
Puliaeva
E V
EV
Barbashov
S F
SF
D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D009206:Myocardium / Q000737:chemistry*; D014335:Tropomyosin / Q000737:chemistry* / Q000235:genetics
D014335:Tropomyosin
Identifikatsiia alpha-tropomiozina serdechnoĭ myshtsy cheloveka dvumernym élektroforezom i sekvenirovaniem.