Selective liquid chromatographic assay for propylthiouracil in plasma.
A liquid chromatographic assay was developed to quantitate propylthiouracil in plasma using an internal standard, 5-propyl-2-thiouracil, of similar structure and physical properties. Caffeine, which coelutes with propylthiouracil, was removed by extraction from serum treated with base. No other compounds were found to interfere in the assay. The drug was extracted from plasma with chloroform with a recovery of 59.4% and the intra- and inter-assay coefficients of variation were 5.7 and 3.3%, respectively. The assay was linear to 3 micrograms/ml with a lower detection limit of 40 ng/ml for a sample volume of 1 ml.
Cannell
G R
GR
Conjoint Internal Medicine Laboratory, Royal Brisbane Hospital, Queensland, Australia.
Williams
J P
JP
Yap
A S
AS
Mortimer
R H
RH
D002110:Caffeine / Q000097:blood; D002725:Chloroform; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D006207:Half-Life; D006801:Humans; D011441:Propylthiouracil / Q000097:blood* / Q000493:pharmacokinetics
D002110:Caffeine; D011441:Propylthiouracil; D002725:Chloroform