Human recombinant interleukin 1 inhibits TSH-stimulated morphological changes in thyroid follicles cultured as semi-organs.
To study the effects of human recombinant interleukin-1 on thyrocytes, we cultured thyroid follicles as semi-organs, each consisting of approximately 10-20 follicles, in the presence or absence of IL-1 alpha or beta. Semi-organ culture reproduces the in vivo environment well. After culture for 2 or 4 days, the follicles were incubated with TSH (10 U/l) for 4 h and fixed for light and electron microscopical examinations. Regardless of the presence or absence of IL-1, follicular structure, polarity, and luminal colloid did not change during culture. In thyroid epithelial cells cultured without IL-1, TSH markedly induced elongation of microvilli and formation of reabsorbed colloid droplets. On the other hand, both IL-1 alpha and beta inhibited these TSH-stimulated changes. The degree of inhibition correlated with the concentration of exposure to IL-1. We conclude that IL-1 inhibits TSH-stimulated morphological changes in thyroid follicles cultured as semi-organs, depending on the concentration of IL-1.
Asakawa
H
H
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Hanafusa
T
T
Oda
Y
Y
Katsura
H
H
Miyagawa
J
J
Mashita
K
K
Kono
N
N
Tarui
S
S
D000818:Animals; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D008854:Microscopy, Electron; D009924:Organ Culture Techniques; D009994:Osmolar Concentration; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins; D013961:Thyroid Gland / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D013972:Thyrotropin / Q000494:pharmacology*; D013997:Time Factors
D007375:Interleukin-1; D011994:Recombinant Proteins; D013972:Thyrotropin