Effect of dimethyl sulphoxide and some antibiotics on cultured human T-lymphoma cells as measured by microcalorimetry.
The influence of dimethyl sulphoxide (I), penicillin/streptomycin (II), gentamicin (III), and amphotericin B (IV) on growing human T-lymphoma cells was measured by microcalorimetry. There was a dose-dependent decrease in the heat production rate of the cells after 24 h of incubation with I in concentrations ranging from 0-2% (v/v). At 3.6%, about half of the cells died. II and III had no effect on the cells after incubation for 6 days, at concentrations from 1 to 10 times that of the normal (50-500 IU/ml; 50-500 micrograms/ml). IV was used in combination with II (50 IU/ml; 50 micrograms/ml) and III (50 micrograms/ml), respectively, at concentrations between 0.25 and 7.5 micrograms/ml. After 6 days of incubation, the results were similar to those obtained with II and III separately.
Lönnbro
P
P
Chemical Centre, University of Lund, Sweden.
Wadsö
I
I
D000666:Amphotericin B / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002151:Calorimetry; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D016399:Lymphoma, T-Cell / Q000378:metabolism* / Q000473:pathology; D010400:Penicillin G / Q000494:pharmacology; D013307:Streptomycin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D003470:Culture Media; D005839:Gentamicins; D000666:Amphotericin B; D010400:Penicillin G; D013307:Streptomycin; D004121:Dimethyl Sulfoxide