Inhibition of IL-1 beta expression in THP-1 cells by probucol and tocopherol.
The cytokine interleukin-1, IL-1, likely plays an important role in the early stages of atherogenesis. The possible action of probucol and tocopherol on the expression and secretion of IL-1 beta was investigated using the human monocytic leukemia cell line, THP-1. Both probucol and D-alpha-tocopherol inhibit the phorbol ester-induced release of IL-1 beta without altering differentiation. Analysis of IL-1 beta mRNA levels revealed that probucol and tocopherol had an inhibitory effect on the activation of expression of the IL-1 beta gene. The data suggest that the beneficial effects of probucol may be related to inhibition of IL-1 at an early phase of atherosclerotic plaque formation.
Akeson
A L
AL
Merrell Dow Research Institute, Cincinnati, OH 45215.
Woods
C W
CW
Mosher
L B
LB
Thomas
C E
CE
Jackson
R L
RL
D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism*; D011341:Probucol / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*
D007375:Interleukin-1; D012333:RNA, Messenger; D014810:Vitamin E; D013755:Tetradecanoylphorbol Acetate; D011341:Probucol