Non-isotopic detection of chromosome 1 in human meiosis and demonstration of disomic sperm nuclei.
Nonradioactive in situ hybridization with the biotin-labeled chromosome 1-specific probe pUC1.77 was performed on human mitotic and meiotic chromosomes, and on sperm nuclei. The streptavidine-horseradish-peroxidase and diaminobenzidine detection system demonstrated heteromorphisms in the 1q12 heterochromatic region, not only in mitotic cells but also in mature sperm heads. The localization of chromosome 1 could be traced through all meiotic stages and in the sperm nuclei. The frequency of chromosome 1 disomy in human sperm, as indicated by two distinct hybridization signals, was calculated to be 0.41%.
Guttenbach
M
M
Institut für Humangenetik der Universität, Biozentrum, Würzburg, Federal Republic of Germany.
Schmid
M
M
D000328:Adult; D000368:Aged; D000782:Aneuploidy; D001710:Biotin; D002467:Cell Nucleus / Q000648:ultrastructure*; D002878:Chromosomes, Human, Pair 1; D015342:DNA Probes; D006801:Humans; D007621:Karyotyping; D008297:Male; D008540:Meiosis; D008938:Mitosis; D013091:Spermatogenesis; D013094:Spermatozoa / Q000166:cytology / Q000648:ultrastructure*
D015342:DNA Probes; D001710:Biotin