Primary structure of two forms of gonadotropin-releasing hormone from brains of the American alligator (Alligator mississippiensis).
Two forms of gonadotropin-releasing hormone (GnRH) have been purified from brains of the American alligator, Alligator mississippiensis, using reverse-phase high-pressure liquid chromatography (HPLC). The concentration of total GnRH was 8.8 ng/g of frozen brain tissue or 21.1 ng per brain. The amino acid sequence of each form of GnRH was determined using automated Edman degradation. The presence of the N-terminal pGlu residue was established by digestion studies with bovine pyroglutamyl aminopeptidase and coelution with synthetic forms of the native peptide. The primary structure of alligator GnRH I is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Gln-Pro-Gly-NH2 and alligator GnRH II is pGlu-His-Trp-Ser-His-Gly-Trp-Tyr-Pro-Gly-NH2.
Lovejoy
D A
DA
Biology Department, University of Victoria, B.C., Canada.
Fischer
W H
WH
Parker
D B
DB
McRory
J E
JE
Park
M
M
Lance
V
V
Swanson
P
P
Rivier
J E
JE
Sherwood
N M
NM
D000489:Alligators and Crocodiles; D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002645:Chickens; D002845:Chromatography; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry / Q000302:isolation & purification*; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry
D010446:Peptide Fragments; D007987:Gonadotropin-Releasing Hormone