title: CD9 antigen on acute non-lymphoid leukemia cells: preferential expression by promyelocytic (M3) subtype.
abstract: A monoclonal antibody (S17-12), previously described to recognize subsets of myelomonocytic cells, is demonstrated to bind CD9 antigen, a surface marker of B-cell precursors, platelets and several non-hematopoietic tissues. The proportion of S17-12, CD9-positive leukemic cells was determined in 102 patients with acute non-lymphocytic leukemia. It was found to be above 75% in 14/22 M3 cases (including 3/3 of microgranular variant) and only in 1/80 cases of different FAB subtype. Complete reactivity (greater than 95%) of leukemic clonogenic cells with CD9 MoAB was restricted to 4/18 M3 cases. Therefore, a high proportion of CD9-positive cells seems to be peculiar to M3 cases. This may help in the diagnosis of cases that lack typical morphological features.
authors.affiliation: Cattedra di Ematologia, Universita' di Torino, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Ferrero
authors.forename: N
authors.initials: N
authors.lastname: Carlesso
authors.forename: E
authors.initials: E
authors.lastname: Gallo
authors.forename: P
authors.initials: P
authors.lastname: Pregno
authors.forename: P
authors.initials: P
authors.lastname: De Fabritiis
authors.forename: M C
authors.initials: MC
authors.lastname: Petti
authors.forename: F
authors.initials: F
authors.lastname: Mandelli
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000378:metabolism / Q000502:physiology*; D001667:Binding, Competitive; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000276:immunology* / Q000473:pathology; D015473:Leukemia, Promyelocytic, Acute / Q000276:immunology* / Q000473:pathology; D008562:Membrane Glycoproteins; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000473:pathology; D060245:Tetraspanin 29; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; C554869:CD9 protein, human; D008562:Membrane Glycoproteins; D060245:Tetraspanin 29