[An immunoenzyme method for demonstrating Coxiella burnetii antigens].
Materials on the development of an enzyme immunoassay (EIA) system for the detection of the antigens of C. burnetii, the causative agent of Q rickettsiosis, are presented. The system is highly specific and effective with respect to both corpuscular antigens of phases 1 and 2 and soluble antigen (lipopolysaccharide). The sensitivity of this method varies within the range 5-100 ng/ml. The effectiveness of EIA as a quantitative (semiquantitative) control test used in the process of the production of Coxiella preparations has been demonstrated.
Gorbachev
E N
EN
Tokarevich
N K
NK
Verbov
V N
VN
Kartseva
N A
NA
Artiukhov
A I
AI
Daĭter
A B
AB
D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D002642:Chick Embryo; D003381:Coxiella / Q000276:immunology*; D005069:Evaluation Studies as Topic; D007106:Immune Sera / Q000302:isolation & purification; D007114:Immunization; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D007074:Immunoglobulin G / Q000302:isolation & purification; D051379:Mice; D011817:Rabbits; D012995:Solubility
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007106:Immune Sera; D007074:Immunoglobulin G
Immunofermentnyĭ metod indikatsii antigenov Coxiella burnetii.