Application of ion pair high performance liquid chromatography to the analysis of porphyrins in clinical samples.
Reversed phase ion pair chromatography is a highly selective separation technique for the determination of free porphyrin carboxylic acids from human materials. Isocratic and gradient elution methods can be used to analyse porphyrin isomers and to establish porphyrin profiles for the biochemical diagnosis of porphyrias. Ion pair high performance liquid chromatography led to the discovery of the atypical isomers II and IV of uroporphyrin and coproporphyrin in human urine. Advantages and limitations of the ion pair technique are discussed.
Jacob
K
K
Institute of Clinical Chemistry, University Hospital Grosshadern, University of Munich, Germany.
Luppa
P
P
D002851:Chromatography, High Pressure Liquid; D003306:Coproporphyrins / Q000097:blood / Q000378:metabolism / Q000652:urine; D005243:Feces / Q000737:chemistry; D006801:Humans; D007477:Ions; D011166:Porphyrins / Q000097:blood / Q000378:metabolism* / Q000652:urine; D011524:Protoporphyrins / Q000097:blood / Q000378:metabolism / Q000652:urine; D013050:Spectrometry, Fluorescence; D014578:Uroporphyrins / Q000097:blood / Q000378:metabolism / Q000652:urine
D003306:Coproporphyrins; D007477:Ions; D011166:Porphyrins; D011524:Protoporphyrins; D014578:Uroporphyrins; C017803:zinc protoporphyrin