Interaction between dual NADPH-dependent reactions of paraquat in mouse brain microsomes.
Paraquat stimulated NADPH-dependent lipid peroxidation in mouse brain microsomes, while it suppressed lipid peroxidation induced by NADPH plus ascorbate. The stimulation of lipid peroxidation by paraquat was inhibited by N-ethylmaleimide (NEM). However, NEM attenuated the inhibitory effect of paraquat on lipid peroxidation elicited by NADPH in combination with ascorbate. These results suggest that an NADPH-dependent reaction which is susceptible to NEM might share an electron from NADPH with the alternative reaction, lipid peroxidation, in the presence of paraquat in mouse brain microsomes.
Hara
S
S
Department of Forensic Medicine, Tokyo Medical College, Japan.
Endo
T
T
Kuriiwa
F
F
Kano
S
S
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D009249:NADP / Q000502:physiology*; D010269:Paraquat / Q000494:pharmacology*
D009249:NADP; D010269:Paraquat