title: Rates of protein turnover at specific sites of the rat incisor periodontal ligament.
abstract: Extraction of rat incisor periodontal ligament using neutral salt solutions was carried out on a site-specific basis following administration of 3H-labelled proline, the incorporation of 3H-proline into soluble proteins of different molecular weights was determined by SDS electrophoresis and scintillation counting. Tracer was incorporated primarily into 3 fractions with Mr = greater than 120K, 100K and 45-65K. Appearance of 3H-proline in low molecular weight components which cross-reacted with antibodies to Type 1 collagen (presumably collagen breakdown products) within 30 minutes of administration suggests a pool of collagen which is rapidly turning over. The rate of maturation of collagen from this soluble to insoluble forms may represent a level of post-translational control within the tissue which may be directly related to tissue morphostasis.
authors.affiliation: Department of Oral Biology, University of Leeds, UK.
authors.forename: J
authors.initials: J
authors.lastname: Kirkham
authors.forename: C
authors.initials: C
authors.lastname: Robinson
authors.forename: R C
authors.initials: RC
authors.lastname: Shore
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003094:Collagen / Q000032:analysis / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007180:Incisor; D008297:Male; D008970:Molecular Weight; D010513:Periodontal Ligament / Q000737:chemistry / Q000378:metabolism*; D011392:Proline / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012967:Sodium Dodecyl Sulfate; D014316:Tritium
chemical_list: D011506:Proteins; D014316:Tritium; D012967:Sodium Dodecyl Sulfate; D003094:Collagen; D011392:Proline