Effect of Aeromonas proteases on the binding of Aeromonas hydrophila strains to connective tissue proteins.
125I-labelled connective tissue protein binding to cells of Aeromonas hydrophila, A. caviae, and A. sobria strains isolated from diseased fish, was correlated with the Aeromonas protease degradation of 125I-labelled collagen types I and IV, fibronectin and laminin, immobilized on tissue culture microtitre plates. An inverse relation between 125I-labelled connective tissue protein binding to cells of Aeromonas strains and proteolytic degradation of immobilized connective tissue proteins by Aeromonas proteases was established. Inhibition of the Aeromonas proteolytic activity by protease inhibitors enhances the 125I-labelled connective tissue protein binding to cells of Aeromonas hydrophila strains. Culture conditions were found to influence both expression of proteolytic activity and binding properties.
Ascencio
F
F
Department of Medical Microbiology, University of Lund, Sweden.
Wadström
T
T
D000333:Aeromonas / Q000201:enzymology* / Q000378:metabolism; D000818:Animals; D001422:Bacterial Adhesion; D001424:Bacterial Infections / Q000382:microbiology / Q000662:veterinary; D003094:Collagen / Q000378:metabolism; D003238:Connective Tissue / Q000378:metabolism*; D003470:Culture Media; D010450:Endopeptidases / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D005393:Fish Diseases / Q000382:microbiology; D005399:Fishes; D007797:Laminin / Q000378:metabolism; D011485:Protein Binding; D013696:Temperature
D003470:Culture Media; D005353:Fibronectins; D007797:Laminin; D003094:Collagen; D010450:Endopeptidases