Influence of topically applied adrenergic agents on cochlear blood flow.
This study was designed to assess the role of adrenergic receptors in the control of cochlear blood flow. Laser Doppler flowmetry was used to determine the effects of adrenergic drugs topically applied to the round window membrane of the cochlea. The relative influence of the various receptor types (alpha 1, alpha 2, beta 1, and beta 2) was examined by a selection of agonists and antagonists. The agonists norepinephrine and epinephrine, which have mixed alpha- and beta-receptor effects, and phenylephrine, a strong alpha 1-agonist, all induced a dose-dependent reduction in cochlear blood flow. The agonists isoproterenol (beta-active), salbutamol (alpha 2-active) had no effect on cochlear blood flow. Of the antagonists, when tested alone, only the selective alpha 1-antagonist prazosin had a direct effect on cochlear blood flow, demonstrating an increase in cochlear blood flow. The selective alpha 2-antagonist idazoxan, the beta-antagonist propranolol, and the unselective alpha-antagonist phentolamine had no effect on cochlear blood flow. Interaction studies of agonists and antagonists were performed to specifically define the receptor subclasses responsible for the cochlear blood flow increases with norepinephrine and epinephrine. The results are consistent with the presence of an alpha 1-adrenergic sympathetic control of cochlear blood flow.
Ohlsén
K A
KA
University of Michigan, Kresge Hearing Research Institute, Ann Arbor 48109-0506.
Baldwin
D L
DL
Nuttall
A L
AL
Miller
J M
JM
D000287:Administration, Topical; D000420:Albuterol / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D001381:Azepines / Q000008:administration & dosage / Q000494:pharmacology; D003051:Cochlea / Q000098:blood supply* / Q000187:drug effects; D004146:Dioxanes / Q000008:administration & dosage / Q000494:pharmacology; D004837:Epinephrine / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D019329:Idazoxan; D007545:Isoproterenol / Q000008:administration & dosage / Q000494:pharmacology; D007834:Lasers; D008297:Male; D009638:Norepinephrine / Q000008:administration & dosage / Q000494:pharmacology; D010646:Phentolamine / Q000008:administration & dosage / Q000494:pharmacology; D010656:Phenylephrine / Q000008:administration & dosage / Q000494:pharmacology; D011224:Prazosin / Q000008:administration & dosage / Q000494:pharmacology; D011433:Propranolol / Q000008:administration & dosage / Q000494:pharmacology; D012405:Round Window, Ear; D013566:Sympathomimetics / Q000008:administration & dosage / Q000494:pharmacology*
D001381:Azepines; D004146:Dioxanes; D013566:Sympathomimetics; D010656:Phenylephrine; D011433:Propranolol; C015603:azepexole; D007545:Isoproterenol; D000420:Albuterol; D009638:Norepinephrine; D011224:Prazosin; D019329:Idazoxan; D004837:Epinephrine; D010646:Phentolamine