title: Comparison of phosphate metabolites of the ocular humors.
abstract: Ocular humors (aqueous humor and vitreous body) were obtained from porcine eyes. Perchloric acid extracts were prepared from aqueous and vitreous aspirates, and phosphorus-31 nuclear magnetic resonance spectroscopy 31P-NMR performed. Resonance signals from four high-energy phosphate groups (phosphocreatine, adenosine tri- and diphosphate and the dinucleotides) and nine low-energy phosphate groups [dihydroxyacetone phosphate, hexose-6-phosphate, alpha-glycerophosphate, fructose-1,6-diphosphate, ethanolamine phosphate, choline phosphate, inorganic orthophosphate (Pi), glycerol-3-phosphorylethanolamine and glycerol-3-phosphorylcholine] were present in the aqueous humor and/or vitreous body. Vitreous body phosphates significantly elevated relative to that of the aqueous humor were: DHAP, Hex 6-P, alpha-GP, Fru 1,6-diP, CP, GPE, PCr, ATP, ADP, and the dinucleotides; only Pi was significantly lower. Six metabolic indices (monoesters, diesters, monoesters/diesters, monoesters/Pi, ATP/Pi, energy modulus) were determined; all vitreous body indices were significantly higher than those of the aqueous humor. In particular, the energy modulus, a measure of overall metabolic energy status, was higher in the vitreous body (0.121 +/- 0.047) than in the aqueous humor (0.05 +/- 0.021).
authors.affiliation: Division of Ophthalmology, Beth Israel Hospital, Boston, Mass.
authors.forename: J V
authors.initials: JV
authors.lastname: Greiner
authors.forename: L A
authors.initials: LA
authors.lastname: Chanes
authors.forename: T
authors.initials: T
authors.lastname: Glonek
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009943:Organophosphorus Compounds / Q000032:analysis*; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus / Q000032:analysis; D013403:Sugar Phosphates / Q000032:analysis; D013552:Swine; D014822:Vitreous Body / Q000737:chemistry*
chemical_list: D009943:Organophosphorus Compounds; D010710:Phosphates; D013403:Sugar Phosphates; D010758:Phosphorus