Determination of osteocalcin in serum by an ultramicro-ELISA with alkaline phosphatase as marker enzyme.
A competitive ELISA for osteocalcin in 10 microliters samples has been developed with alkaline phosphatase as a marker enzyme. The assay for osteocalcin has an inter-assay and intra-assay variability of 13% and 12%, respectively; the limit of detection is 3.6 pg per assay. The assay was used to measure osteocalcin serum levels in patients with chronic hemodialysis and several bone diseases. The method for the purification of osteocalcin is also described.
Wenz
I
I
Department of Medicine, Friedrich-Schiller University, Jean, FRG.
Reissmann
R
R
Börnig
H
H
D000469:Alkaline Phosphatase; D000818:Animals; D001847:Bone Diseases / Q000097:blood; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008832:Microchemistry; D015675:Osteocalcin / Q000097:blood* / Q000302:isolation & purification; D006435:Renal Dialysis
D015675:Osteocalcin; D000469:Alkaline Phosphatase