Effect of parathyroid hormone on release of interleukin 1 and interleukin 6 from cultured mouse osteoblastic cells.
This study was undertaken to examine the possibility that parathyroid hormone promotes the production of interleukin 1 and 6 in MC3T3-E1 osteoblastic cells derived from mouse calvaria. The cells were incubated in serum-free medium with or without synthetic human parathyroid hormone(1-34). The concentrations of interleukin 1 and 6 in the culture medium were determined by using specific bioassay. The cells cultured without parathyroid hormone for 24 hr released both of interleukins, and parathyroid hormone stimulated the release in a dose-dependent manner. When the cells were cultured with 10(-6) M parathyroid hormone, the release of both interleukins from the cells remained higher than control up to 144 hr. These results suggest that interleukin 1 and 6 release stimulated by parathyroid hormone may be involved in the bone resorbing activity of the hormone.
Li
N H
NH
Department of Geriatrics, Faculty of Medicine, University of Tokyo, Japan.
Ouchi
Y
Y
Okamoto
Y
Y
Masuyama
A
A
Kaneki
M
M
Futami
A
A
Hosoi
T
T
Nakamura
T
T
Orimo
H
H
D000818:Animals; D000906:Antibodies; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects; D007375:Interleukin-1 / Q000276:immunology / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology*; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D019379:Teriparatide; D013997:Time Factors
D000906:Antibodies; D007375:Interleukin-1; D015850:Interleukin-6; D010281:Parathyroid Hormone; D010446:Peptide Fragments; D019379:Teriparatide