Partial purification of esterases from rabbit serum and their use in regioselective deacylations of sugars.
14C-Labelled methyl 2,6-di-O-pivaloyl-alpha-D-glucopyranoside was used as a substrate for the detection of esterase activity in the isolation of esterase II from rabbit serum. On treatment of methyl 2,6-di-O-pivaloyl-alpha-D-glucopyranoside with rabbit serum and esterase II, the 6-O-pivaloyl group was removed selectively. Likewise, the 6-O-pivaloyl group was removed selectively from methyl 2-acetamido-2-deoxy-3,4,6-tri-O-pivaloyl-alpha-D-glucopyranoside.
Tomić
S
S
Department of Radioimmunology, Institute of Immunology, Zagreb, Yugoslavia.
Trescec
A
A
Ljevaković
D
D
Tomasić
J
J
D000818:Animals; D050260:Carbohydrate Metabolism; D004950:Esterases / Q000097:blood / Q000302:isolation & purification*; D015394:Molecular Structure; D010421:Pentanoic Acids / Q000378:metabolism; D011817:Rabbits
D010421:Pentanoic Acids; C005566:pivalic acid; D004950:Esterases