In-vitro studies on 'spare' human preimplantation embryos in culture.
Methods previously used for the biopsy of preimplantation mouse embryos have been applied to individual 'spare' human embryos. Early cleavage-stage human embryos have been cultured and individual blastomeres removed following zonae thinning or drilling. Embryos have also been cultured to the blastocyst stage for the biopsy of three to five trophectoderm cells. Both the biopsied embryo and the biopsied cells have been allowed to develop and/or grow in vitro.
Muggleton-Harris
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MRC Experimental Embryology and Teratology Unit, St George's Hospital Medical School, Tooting, London, UK.
Findlay
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D000818:Animals; D001706:Biopsy / Q000379:methods*; D001755:Blastocyst / Q000166:cytology*; D046508:Culture Techniques; D005353:Fibronectins / Q000494:pharmacology; D005780:Gelatin / Q000494:pharmacology; D006801:Humans; D007552:Isotonic Solutions / Q000494:pharmacology; D051379:Mice; D011296:Prenatal Diagnosis / Q000379:methods; D015044:Zona Pellucida / Q000187:drug effects
D005353:Fibronectins; D007552:Isotonic Solutions; C037692:Tyrode's solution; D005780:Gelatin