Effects of the lipase inhibitors, Triton WR-1339 and tetrahydrolipstatin, on the synthesis and secretion of lipids by rat hepatocytes.
The lipase inhibitors, Triton WR-1339 and tetrahydrolipstatin, were incubated with rat hepatocytes. Triton WR-1339 increased the recovery of triacylglycerol in the hepatocytes and incubation medium by 31% and 38%, respectively. Tetrahydrolipstatin decreased the accumulation of newly synthesized, and of total triacylglycerol in the medium. This compound might be useful in determining mechanisms involved in intracellular triacylglycerol metabolism and the secretion of very low density lipoproteins.
Hermier
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Department of Biochemistry, Faculty of Medicine, University of Alberta, Edmonton, Canada.
Hales
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Brindley
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DN
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D007783:Lactones / Q000494:pharmacology*; D008049:Lipase / Q000037:antagonists & inhibitors*; D050356:Lipid Metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D000077403:Orlistat; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats
D003470:Culture Media; D007783:Lactones; D011092:Polyethylene Glycols; D000077403:Orlistat; D008049:Lipase; C016811:tyloxapol