Pannorin, a new 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor produced by Chrysosporium pannorum.
Pannorin, a naphthopyrone that inhibits 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, the rate-limiting enzyme in cholesterol synthesis, was isolated from a culture broth of Chrysosporium pannorum M10539 by solvent extraction, Bio-Gel P-6 column chromatography and reverse phase HPLC (Silica ODS). Spectroscopic analyses of the compound yielded 4,8,10-trihydroxy-5-methyl-2H-naphtho[1,2-b]pyran-2-one as the proposed structure. Pannorin inhibited HMG-CoA reductase and in vitro sterol synthesis 50% at a concentration of 160 microM.
Ogawa
H
H
Department of Applied Biological Science, Tokyo Noko University, Japan.
Hasumi
K
K
Sakai
K
K
Murakawa
S
S
Endo
A
A
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002912:Chrysosporium / Q000145:classification / Q000378:metabolism*; D005285:Fermentation; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D008099:Liver / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009284:Naphthols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011753:Pyrones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D013055:Spectrophotometry, Infrared
D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D009284:Naphthols; D011753:Pyrones; C070543:pannorin