Amino acid sequence of a myotoxin from venom of the eastern diamondback rattlesnake (Crotalus adamanteus).
The complete amino acid sequence of a myotoxin isolated from Crotalus adamanteus venom (CAM-toxin) was determined. The total number of amino acid residues was 45, giving a mol. wt of 5202. The amino acid sequence was compared with those of previously determined Crotalus myotoxins. The CAM-toxin shows a surprisingly close relationship to those of peptide c (C. v. helleri), myotoxin a (C. v. viridis), crotamine (C. d. terrificus), myotoxin I and II (C. v. concolor) with homologies of approximately 98, 83, 90, 95 and 91%, respectively. The hydropathy profile of the CAM-toxin is almost identical to peptide c and myotoxin I, as can be assumed from the sequence homology. Despite the absence of any close sequence homology with the myotoxic phospholipase A2 enzymes, CAM-toxin contains a similar cationic myotoxic region at residues 2-10 as found in the other five myotoxic peptides.
Samejima
Y
Y
Faculty of Pharmacy, Hoshi University, Tokyo, Japan.
Aoki
Y
Y
Mebs
D
D
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D003435:Crotalid Venoms / Q000737:chemistry*; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000737:chemistry*; D010446:Peptide Fragments / Q000032:analysis; D030162:Reptilian Proteins; D012689:Sequence Homology, Nucleic Acid; D013329:Structure-Activity Relationship
D000596:Amino Acids; D003435:Crotalid Venoms; D009498:Neurotoxins; D010446:Peptide Fragments; D030162:Reptilian Proteins; C013144:myotoxic component of rattlesnake venom; D003488:Cyanogen Bromide