title: Quantitative analysis of electron energy-loss spectra from ultrathin-sectioned biological material. I. Optimization of the background-fit with the use of Bio-standards.
abstract: A computer program for quantitative spectral analysis is proposed for the elemental analysis of biological material by electron energy-loss spectroscopy in a conventional transmission electron microscope, the Zeiss EM902. Bio-standards are used to test the performance of this program. The application of a simplex optimization method for curve-fitting is proposed to separate the ionization edge from the background. Making use of Ce-, Ca- and Fe-bio-standards, this method is compared with Egerton's well-known two-area method.
authors.affiliation: AEM Unit, Clinical Pathological Institute I, Rotterdam, The Netherlands.
authors.forename: C W
authors.initials: CW
authors.lastname: Sorber
authors.forename: G A
authors.initials: GA
authors.lastname: Ketelaars
authors.forename: E S
authors.initials: ES
authors.lastname: Gelsema
authors.forename: J F
authors.initials: JF
authors.lastname: Jongkind
authors.forename: W C
authors.initials: WC
authors.lastname: De Bruijn
mesh_terms: D002118:Calcium / Q000737:chemistry; D002563:Cerium / Q000737:chemistry; D007091:Image Processing, Computer-Assisted; D007501:Iron / Q000737:chemistry; D008854:Microscopy, Electron / Q000379:methods*; D008867:Microtomy; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D002563:Cerium; D007501:Iron; D002118:Calcium