A Brassica napus mRNA expressed specifically in developing microspores.
The I3 cDNA isolated from a library made from 2-4 mm (immature) anthers of Brassica napus shows microspore-specific expression. Homologous transcripts are detected in buds and anthers of male-fertile plants, but not in green tissues, roots, or in cytoplasmic male-sterile buds. High expression of the transcript is limited to microspores entering and undergoing mitosis. The predicted peptide sequence of the cDNA shows an unusual repeated alanine/proline motif at the C-terminus, which may be of importance in the native protein structure.
Roberts
M R
MR
Department of Botany, University of Leicester, U.K.
Robson
F
F
Foster
G D
GD
Draper
J
J
Scott
R J
RJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005718:Gametogenesis; D015870:Gene Expression; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
D010940:Plant Proteins; D012333:RNA, Messenger; D004247:DNA