Synthetic oligonucleotide probes differentiate respiratory syncytial virus subgroups in a nucleic acid hybridization assay.
A new approach to respiratory syncytial virus subgroup differentiation has been developed on the basis of the hybridization of subgroup-specific synthetic oligonucleotides with viral mRNA. Two oligonucleotide probes were designed from the nucleotide sequences of an A and a B subgroup respiratory syncytial virus glycoprotein G gene. All 28 virus isolates tested were correctly classified by subgroup with these probes.
Sullender
W M
WM
Department of Pediatrics, University of Alabama, Birmingham School of Medicine 35294.
Wertz
G W
GW
D001483:Base Sequence; D015342:DNA Probes; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012136:Respiratory Syncytial Viruses / Q000145:classification / Q000235:genetics*
D015342:DNA Probes; D012333:RNA, Messenger; D012367:RNA, Viral