title: Anthracycline antibiotic 2-hydroxyaclacinomycins. I. 2-Hydroxyaclacinomycin-producing recombinant obtained from aclacinomycin-blocked mutants of Streptomyces galilaeus by a technique of protoplast fusion.
abstract: The technique of protoplast fusion which optimized prototrophic recombination in aclacinomycin-producing Streptomyces galilaeus was studied and applied to the construction of new anthracycline analog-producing recombinant upon genetic cross of two specific mutants blocked in aclacinomycin biosynthesis. Thus, 2-hydroxyaclacinomycin-producing recombinant was obtained by the protoplast fusion.
authors.affiliation: Central Research Laboratories, Mercian Corporation, Kanagawa, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Yoshimoto
authors.forename: O
authors.initials: O
authors.lastname: Johdo
authors.forename: T
authors.initials: T
authors.lastname: Ishikura
authors.forename: T
authors.initials: T
authors.lastname: Takeuchi
mesh_terms: D015250:Aclarubicin / Q000031:analogs & derivatives*; D000903:Antibiotics, Antineoplastic; D009154:Mutation; D011995:Recombination, Genetic; D013302:Streptomyces / Q000235:genetics*
chemical_list: D000903:Antibiotics, Antineoplastic; D015250:Aclarubicin; C003424:2-hydroxyaclacinomycin A