title: Role of the C-terminus in the biological activity of human interleukin 5.
abstract: Alterations of the C-terminal amino acid sequence of recombinant human interleukin 5 (rhIL-5) caused significant changes in its biological activity. Removal of eight amino acids from the C-terminus of rhIL-5 by CNBr treatment led to a complete loss of biological activity as determined by the BCL1 cell IgM-inducing assay. Oxidation of Met residue located at C-terminus also resulted in a loss of activity. These results suggest that the C-terminal amino acids of rhIL-5 are crucial for its biological actions.
authors.affiliation: Suntory Institute for Biomedical Research, Osaka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kodama
authors.forename: N
authors.initials: N
authors.lastname: Tsuruoka
authors.forename: M
authors.initials: M
authors.lastname: Tsujimoto
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002700:Chloramines; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis; D007202:Indicators and Reagents; D015848:Interleukin-5 / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000302:isolation & purification / Q000494:pharmacology; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D014105:Tosyl Compounds; D014162:Transfection
chemical_list: D002700:Chloramines; D007075:Immunoglobulin M; D007202:Indicators and Reagents; D015848:Interleukin-5; D010446:Peptide Fragments; D011994:Recombinant Proteins; D014105:Tosyl Compounds; C016300:chloramine-T; D003488:Cyanogen Bromide