title: Colonization control of lactose-fermenting Salmonella typhimurium in young broiler chickens by use of dietary lactose.
abstract: Inclusion of lactose in the diets of chickens has been determined to reduce cecal colonization with Salmonella typhimurium. We hypothesized, therefore, that dietary lactose may be a practical means for reducing the prevalence of Salmonella contamination of chicken products. Because some strains of Salmonella are atypical and ferment lactose, we investigated the effects of dietary lactose on cecal colonization with lactose-fermenting S typhimurium. Broiler chicks were inoculated intracloacally with Lac+ S typhimurium selected for resistance to novobiocin and rifampicin. The chicks also were inoculated orally with certain anaerobes that do not effectively inhibit colonization by S typhimurium, but do appear essential for lactose mediated inhibition of cecal colonization. Control chicks were not given dietary lactose, and chicks in the experimental group were fed a diet containing 7% lactose. Enumeration of Lac+ S typhimurium in cecal contents revealed dietary lactose to be effective at controlling this organism. Control was correlated with changes in cecal pH and increases in undissociated volatile fatty acids, especially propionic acid.
authors.affiliation: US Department of Agriculture, Agricultural Research Service, College Station, TX 77845.
authors.forename: R L
authors.initials: RL
authors.lastname: Ziprin
authors.forename: M H
authors.initials: MH
authors.lastname: Elissalde
authors.forename: A
authors.initials: A
authors.lastname: Hinton
authors.forename: R C
authors.initials: RC
authors.lastname: Beier
authors.forename: G E
authors.initials: GE
authors.lastname: Spates
authors.forename: D E
authors.initials: DE
authors.lastname: Corrier
authors.forename: T G
authors.initials: TG
authors.lastname: Benoit
authors.forename: J R
authors.initials: JR
authors.lastname: DeLoach
mesh_terms: D000818:Animals; D002432:Cecum / Q000737:chemistry / Q000382:microbiology; D002645:Chickens; D004040:Dietary Carbohydrates / Q000627:therapeutic use; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000032:analysis; D019344:Lactic Acid; D007785:Lactose / Q000378:metabolism / Q000627:therapeutic use*; D008297:Male; D011201:Poultry Diseases / Q000382:microbiology / Q000517:prevention & control*; D011422:Propionates / Q000032:analysis; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000517:prevention & control*; D012486:Salmonella typhimurium / Q000254:growth & development / Q000378:metabolism*
chemical_list: D004040:Dietary Carbohydrates; D007773:Lactates; D011422:Propionates; D019344:Lactic Acid; D007785:Lactose; C029658:propionic acid