title: High-level resistance to cefotaxime and ceftazidime in Klebsiella pneumoniae isolates from Cleveland, Ohio.
abstract: Two isolates of Klebsiella pneumoniae possessing both TEM-1 and SHV-2 beta-lactamases were isolated from patients at the Cleveland Clinic in 1988. The beta-lactamases were discriminated and identified by using substrate hydrolysis data and an isoelectric focusing procedure in which the gel was overlaid with beta-lactamase inhibitors.
authors.affiliation: Department of Medical Microbiology, Creighton University School of Medicine, Omaha, Nebraska 68178.
authors.forename: K S
authors.initials: KS
authors.lastname: Thomson
authors.forename: C C
authors.initials: CC
authors.lastname: Sanders
authors.forename: J A
authors.initials: JA
authors.lastname: Washington
mesh_terms: D002439:Cefotaxime / Q000494:pharmacology*; D002442:Ceftazidime / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D006801:Humans; D007525:Isoelectric Focusing; D007710:Klebsiella Infections / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000201:enzymology; D009820:Ohio; D010957:Plasmids; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D065093:beta-Lactamase Inhibitors; D002442:Ceftazidime; D001618:beta-Lactamases; D002439:Cefotaxime