Experimental hemorrhage and blunt trauma do not increase circulating tumor necrosis factor.
Tumor necrosis factor (TNF) is a potent cytokine mediator of the shock states associated with sepsis and burn injury. This experimental study was done to determine whether circulating TNF plays a major role in the vasomotor collapse seen following experimental hemorrhage and blunt injury. Twenty anesthetized pigs were divided into two groups. Ten animals were bled 60% of their calculated blood volume in 15 minutes. Animals in Group IA (n = 5) had no treatment, and Group IB animals (n = 5) were given twice the shed volume as crystalloid 30 minutes after hemorrhage. The other animals, groups IIa and IIb (n = 5 each), were first subjected to a blunt injury to the thigh sufficient to cause a midshaft femur fracture, then bled and similarly treated. In both groups, mean arterial pressure (MAP), cardiac output (CO), and serum TNF activity by L929 bioassay were measured at 15-minute intervals for 120 minutes after hemorrhage or hemorrhage and blunt injury. An additional three animals were infused with 4 x 10(8)/kg heat-killed E. coli to validate the TNF assay. All bled animals sustained a fall in MAP and CO to a mean of 33% of baseline values, with or without fracture. Group IB and IIB animals responded to fluid resuscitation by restoration of MAP and CO to 85%-97% of the baseline values. Tumor necrosis factor was not detectable before injury and remained undetectable in all these animals during the 120 minutes of the experiment despite hemorrhage alone or combined hemorrhage and blunt trauma, with or without fluid resuscitation. The test animals receiving the E. coli responded with markedly elevated TNF levels, which peaked at 90 minutes after injection.(ABSTRACT TRUNCATED AT 250 WORDS)
Stylianos
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Division of Pediatric Surgery, Tufts University School of Medicine, Boston, MA 02111.
Wakabayashi
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G
Gelfand
J A
JA
Harris
B H
BH
D000818:Animals; D005440:Fluid Therapy; D006470:Hemorrhage / Q000097:blood* / Q000150:complications / Q000628:therapy; D012769:Shock / Q000097:blood / Q000209:etiology; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*; D014949:Wounds, Nonpenetrating / Q000097:blood* / Q000150:complications
D014409:Tumor Necrosis Factor-alpha