Candida albicans and three other Candida species contain an elongation factor structurally and functionally analogous to elongation factor 3.
A cell-free poly(U)-dependent translation elongation system from Candida albicans is ATP-dependent due to the presence of an elongation factor 3 (EF3)-like activity. Saccharomyces cerevisiae ribosomes added to a C. albicans postribosomal supernatant (PRS) supported poly(U)-dependent elongation, suggesting that the C. albicans lysate contained a soluble translation factor functionally analogous to the S. cerevisiae translation factor EF-3. The presence of EF-3 in C. albicans was confirmed by Western blotting using an antibody raised against S. cerevisiae EF-3. This antibody was also used to screen a selection of Candida species, all of which possessed EF-3 with molecular mass in the range of 110-130 kDa.
Colthurst
D R
DR
Biological Laboratory, University of Kent, Canterbury, U.K.
Santos
M
M
Grant
C M
CM
Tuite
M F
MF
D002176:Candida albicans / Q000032:analysis* / Q000276:immunology; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D010445:Peptide Elongation Factors / Q000737:chemistry / Q000302:isolation & purification* / Q000502:physiology; D014176:Protein Biosynthesis; D029701:Saccharomyces cerevisiae Proteins
D005656:Fungal Proteins; D010445:Peptide Elongation Factors; D029701:Saccharomyces cerevisiae Proteins; C111584:YEF3 protein, S cerevisiae