Effect of magnesium ions on the inhibition of S-adenosylmethionine decarboxylase from Escherichia coli by [2-(amino-oxy)ethyl](5'-deoxyadenosin-5'-yl)(methyl)sulphonium .
[2-(Amino-oxy)ethyl](5'-deoxyadenosin-5'-yl)(methyl)sulphonium+ ++, the amino-oxy analogue of decarboxylated S-adenosylmethionine, is a potent irreversible inhibitor of Escherichia coli S-adenosylmethionine decarboxylase [Khomutov, Zavalova, Syrku, Artamonova & Khomutov (1983) Bioorg. Khim. 9, 130-131; Artamonova, Zavalova, Khomutov & Khomutov (1986) Bioorg. Khim. 12, 206-212]. We have shown that Mg2+ ions are required for the irreversible inhibition of the decarboxylase, and that S-adenosylmethionine protects against this inhibition.
Weitkamp
E L
EL
Department of Biochemistry, University of Cambridge, U.K.
Dixon
H B
HB
Khomutov
A R
AR
Khomutov
R M
RM
D012437:Adenosylmethionine Decarboxylase / Q000037:antagonists & inhibitors*; D003839:Deoxyadenosines / Q000494:pharmacology*; D004926:Escherichia coli / Q000201:enzymology; D008274:Magnesium / Q000494:pharmacology*; D011485:Protein Binding; D012436:S-Adenosylmethionine / Q000378:metabolism
D003839:Deoxyadenosines; D012436:S-Adenosylmethionine; C042768:S-(5'-deoxyadenosyl-5')-S-methylthioethylhydroxylamine; D012437:Adenosylmethionine Decarboxylase; D008274:Magnesium