Inositol biosynthesis: Candida albicans and Saccharomyces cerevisiae genes share common regulation.
The Candida albicans inositol biosynthetic gene and its regulation have been studied. The gene, CalNO1, was cloned on a multicopy vector by complementation of a Saccharomyces cerevisae mutant strain. Southern blot analysis established that the cloned DNA was C. albicans genomic DNA in origin; neither rearrangements nor pseudogenes were evident. Blot hybridization analysis using RNA isolated from C. albicans revealed that a single RNA species (1.8 kilobases) was homologous to the cloned DNA fragment. The steady-state levels of these transcripts were shown to be regulated in response to inositol in the growth media. In addition, the steady-state levels of the RNA encoded by the cloned C. albicans DNA present in S. cerevisiae on a plasmid (YRpCalNO1) were regulated in response to exogenously provided inositol. The cloned C. albicans DNA fragment was shown to restore inositol-1-phosphate synthase activity to a S. cerevisiae mutant strain defective in this enzyme. This activity was also shown to be regulated in response to the presence of inositol in the growth media.
Klig
L S
LS
Glaxo Institute for Molecular Biology S.A., Geneva, Switzerland.
Antonsson
B
B
Schmid
E
E
Friedli
L
L
D015152:Blotting, Northern; D015139:Blotting, Southern; D002176:Candida albicans / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003470:Culture Media; D004271:DNA, Fungal / Q000737:chemistry; D015966:Gene Expression Regulation, Fungal; D007294:Inositol / Q000096:biosynthesis* / Q000235:genetics; D007295:Inositol Phosphates / Q000096:biosynthesis / Q000235:genetics; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis / Q000096:biosynthesis; D012331:RNA, Fungal / Q000378:metabolism; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
D003470:Culture Media; D004271:DNA, Fungal; D007295:Inositol Phosphates; D010743:Phospholipids; D012331:RNA, Fungal; C002647:inositol 1-phosphate; D007294:Inositol