Presence of type I and VI collagen mRNAs in endothelial cells in cutaneous neurofibromas.
In situ hybridization and peroxidase anti-peroxidase immunodetection were used in the same tissue sections to elucidate the spatial distribution of collagen gene expression in cutaneous neurofibromas, particularly in relation to blood vessels; the latter structures were identified by the presence of factor VIII-related antigen. The data indicate a clear relationship between the vascular structures and sites of locally elevated expression of type I and VI collagen genes. Specifically, some, but not all, blood vessels were surrounded by stromal cells highly active in expressing pro alpha 1(I) and alpha 2(VI) collagen genes. Furthermore, these genes were expressed by a subpopulation of endothelial cells within the walls of blood vessels traversing the lesion. To quantitate the overall expression of five genetically distinct collagen genes in cutaneous neurofibromas, we performed Northern analyses and slot blot hybridizations with pro alpha 1 (I), pro alpha 2 (I), pro alpha 1 (III), pro alpha 1 (IV) and alpha 2(VI) collagen cDNAs. Although the mRNA levels for all five genes were slightly increased in neurofibroma tissue, only the abundance of alpha 2(VI) collagen mRNAs was significantly elevated, as compared with normal skin. We conclude that endothelial cell populations with different levels of collagen gene expression exist within cutaneous neurofibromas: some are actively expressing type I and VI collagen genes, whereas in other the expression of these genes is effectively down-regulated. The markedly elevated steady-state levels of type VI collagen mRNAs suggest that synthesis of type VI collagen may contribute to the growth and architecture of cutaneous neurofibromas.
Sollberg
S
S
Department of Dermatology, Jefferson Medical College, Philadelphia, Pennsylvania.
Muona
P
P
Lebwohl
M
M
Peltonen
J
J
Uitto
J
J
D003094:Collagen / Q000235:genetics*; D015342:DNA Probes; D004730:Endothelium, Vascular / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D009455:Neurofibroma / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis*; D012878:Skin Neoplasms / Q000235:genetics*
D015342:DNA Probes; D012333:RNA, Messenger; D003094:Collagen