DNA flow cytometry of large-bowel biopsies showing that adenoma tissue predicts the ultimate nature of the lesions.
Ploidy of neoplastic large-bowel cells in lesions from which biopsies showed only adenoma tissue by histopathologic examination was investigated retrospectively by DNA flow cytometry. Biopsy and resected-specimen blocks gave interpretable ploidy profiles from 83 lesions. The expectation that adenomas will show diploidy in biopsies and that adenocarcinomas will show aneuploidy in biopsies was fulfilled in 80% of the lesions. In addition, four lesions with diploid biopsies were polyps with minimal invasion. Thus, diploidy would have correctly predicted that local resection was adequate treatment for 62 lesions and conversely that cancer resection was the appropriate treatment for 8 lesions, for an overall predictive success rate of 87%. In conjunction with endoscopic, radiologic and other data, DNA flow cytometry of biopsies from apparently benign large-bowel lesions could become a useful addition to therapeutic decision-making.
Frei
J V
JV
Department of Pathology, University of Western Ontario, London.
Troster
M M
MM
Cramer
H M
HM
Martinez
V J
VJ
Cook
M
M
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology / Q000601:surgery; D000782:Aneuploidy; D001706:Biopsy; D003111:Colonic Polyps / Q000235:genetics / Q000473:pathology / Q000601:surgery; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology / Q000601:surgery; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D004171:Diploidy; D005434:Flow Cytometry; D006801:Humans; D007417:Intestinal Polyps / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D007420:Intestine, Large / Q000473:pathology*; D011003:Ploidies; D011336:Probability; D012189:Retrospective Studies
D004273:DNA, Neoplasm