Localization of heterogeneous nuclear ribonucleoprotein in the interphase nuclear matrix core filaments and on perichromosomal filaments at mitosis.
Although heterogeneous nuclear RNA (hnRNA) has been localized to the core filament substructure of the nuclear matrix, its precise location in the filament network has been unknown. The fA12 monoclonal antibody can localize, at high resolution, hn ribonucleoproteins (hnRNPs) and, presumably, hnRNA. Gold bead immunolabeling of resinless electron microscopy sections showed the fA12 antigens were in the fibrogranular material enmeshed in the filament network and not in the filaments themselves. At mitosis, hnRNP antigens became dispersed into a halo surrounding the chromosomes and spindle poles. Immunogold staining showed fA12 stained fibrogranular material associated with perichromosomal and pericentriolar filaments distinct from the mitotic spindle fibers. fA12 also labeled the midbody remaining after cytokinesis.
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Department of Biology, Massachuseets Institute of Technology, Cambridge 02139.
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Penman
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D002460:Cell Line; D002877:Chromosomes, Human / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells / Q000166:cytology / Q000648:ultrastructure; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006801:Humans; D007382:Intermediate Filaments / Q000648:ultrastructure*; D007399:Interphase; D016253:Microscopy, Immunoelectron; D008938:Mitosis; D015530:Nuclear Matrix / Q000648:ultrastructure*; D012332:RNA, Heterogeneous Nuclear / Q000032:analysis*; D012261:Ribonucleoproteins / Q000032:analysis*
D034441:Heterogeneous-Nuclear Ribonucleoproteins; D012332:RNA, Heterogeneous Nuclear; D012261:Ribonucleoproteins