title: Apparatus for gel electrophoresis with continuous monitoring of individual DNA molecules by video epifluorescence microscopy.
abstract: Apparatus for gel electrophoresis with continuous scanning by video epifluorescence microscopy was constructed and applied to measurements on individual DNA molecules in the size range of several hundred kilobase pairs. The gel electrophoresis apparatus comprises a horizontal glass cell and several commercially available components--an epifluorescence microscope, an image intensifier, two video cameras, a monitor, videotape recorder and voltmeter. Data in the form of videotapes can be subsequently analyzed on a computer graphics work station.
authors.affiliation: Section on Macromolecular Analysis, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: N J
authors.initials: NJ
authors.lastname: Rampino
authors.forename: A
authors.initials: A
authors.lastname: Chrambach
mesh_terms: D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation*; D008856:Microscopy, Fluorescence; D014743:Videotape Recording
chemical_list: D004247:DNA