Analysis of proteins expressed at the time of murine organogenesis.
Two-dimensional electrophoretograms were prepared from wild-type C57BL/6J embryos from day 7.5 through day 9.0 of development. This time period encompasses a critical window of development as the embryo traverses from an egg cylinder through major organogenesis. Consequently, we term this resource MOPED (for mouse organogenesis protein electrophoresis database). By resolving and analyzing the behavior of approximately 1,000 polypeptides per time point, we were able to track many of these polypeptides through this time period in development. Of special note was a burst of induced protein synthesis that was observed in mouse embryos development. Polypeptides observed in mouse embryos that match those identified previously in mouse fibroblasts were noted. Two of them (the intermediate filament-associated protein and tropomyosin-4) were significantly altered in 8.5 day embryos. As more polypeptides are designated, it will be possible to expand the known proteins in the database. MOPED establishes the patterns of synthesis of a large number of polypeptides during a crucial period of development. Thus MOPED is designed to analyze proteins relevant to mouse embryogenesis in the future.
Van Keuren
M L
ML
Department of Pediatrics, University of Michigan School of Medicine, Ann Arbor 48109-0650.
Iacob
R A
RA
Kurnit
D M
DM
D000818:Animals; D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional; D004622:Embryo, Mammalian / Q000378:metabolism*; D005314:Embryonic and Fetal Development; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000032:analysis*; D011247:Pregnancy; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis*; D014335:Tropomyosin / Q000096:biosynthesis*
D010455:Peptides; D011506:Proteins; D014335:Tropomyosin