Extracellular administration of lactate dehydrogenase and its effects on human plaque pH and acid anion concentrations.
The effect of lactate dehydrogenase (LDH) on human plaque pH and acid anion concentrations in vivo was investigated. Rinsing with sucrose solutions supplemented with LDH (1 or 2 U/ml) gave rise to reduced pH fall, decreased cH area and an increase in the mean minimum pH when compared with rinsing with sucrose only (p less than 0.05). Mean acid anion estimations showed that plaque fluid concentrations of lactate, acetate and proprionate significantly decrease (p less than 0.05) following rinsing with sucrose containing LDH at both levels and decreases in succinate when 1 U/ml LDH was present, whereas formate concentrations were only significantly lower when 2 U/ml LDH was added to sucrose rinses. It is thought that the ability of LDH to produce an alteration in the acid end-products of plaque metabolism may provide some protection against dental caries.
Higham
S M
SM
Department of Clinical Dental Sciences, School of Dentistry, University of Liverpool, UK.
Edgar
W M
WM
D000085:Acetates / Q000032:analysis; D000143:Acids / Q000378:metabolism; D000328:Adult; D003773:Dental Plaque / Q000737:chemistry / Q000378:metabolism / Q000503:physiopathology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000008:administration & dosage / Q000494:pharmacology*; D007773:Lactates / Q000032:analysis; D008297:Male; D010710:Phosphates / Q000032:analysis; D011422:Propionates / Q000032:analysis; D013395:Sucrose / Q000008:administration & dosage / Q000378:metabolism*; D013997:Time Factors
D000085:Acetates; D000143:Acids; D007773:Lactates; D010710:Phosphates; D011422:Propionates; D013395:Sucrose; D007770:L-Lactate Dehydrogenase