Use and detection of nonradioactive iodine-labeled antibodies for immunoassay.
Traditionally radioactively labeled iodine has been used as a reporter group for the detection of antibodies in immunoassay. We have recently developed a microassay system for the detection of very low concentrations of iodide which eliminates the need for the use of radiolabeled iodine (O'Kennedy, R. et al. 1989, Anal. Biochem. 179, 138-144). The successful application of this assay for the detection of mouse IgG is described. The performance of this system compared with enzyme immunoassay was evaluated.
O'Kennedy
R
R
School of Biological Sciences, Dublin City University, Ireland.
Keating
P
P
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis*; D007202:Indicators and Reagents; D007455:Iodine / Q000032:analysis*; D051379:Mice; D013388:Succinimides
D000906:Antibodies; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D013388:Succinimides; C022581:Bolton-Hunter reagent; D007455:Iodine