title: Induction of neurite outgrowth by v-src mimics critical aspects of nerve growth factor-induced differentiation.
abstract: PC12 cells treated with nerve growth factor (NGF) or infected with Rous sarcoma virus differentiate into sympathetic, neuronlike cells. To compare the differentiation programs induced by NGF and v-src, we have established a PC12 cell line expressing a temperature-sensitive v-src protein. The v-src-expressing PC12 cell line was shown to elaborate neuritic processes in a temperature-inducible manner, indicating that the differentiation process was dependent on the activity of the v-src protein. Further characterization of this cell line, in comparison with NGF-treated PC12 cells, indicated that the events associated with neurite outgrowth induced by these two agents shared features but could be distinguished by others. Both NGF- and v-src-induced neurite outgrowths were reversible. In addition, NGF and v-src could prime PC12 cells for NGF-induced neurite outgrowth, and representative early and late NGF-responsive genes were also induced by v-src. However, unlike NGF-induced neurite growth, v-src-induced neurite outgrowth was not blocked at high cell density. A comparison of phosphotyrosine containing-protein profiles showed that v-src and NGF each increase tyrosine phosphorylation of multiple cellular proteins. There was overlap in substrates; however, both NGF-specific and v-src-specific tyrosine phosphorylations were observed. One protein which was found to be phosphorylated in both the NGF- and v-src-induced PC12 cells was phospholipase C-gamma 1. Taken together, these results suggest that v-src's ability to function as an inducing agent may be a consequence of its ability to mimic critical aspects of the NGF differentiation program and raise the possibility that Src-like tyrosine kinases are involved in mediating some of the events triggered by NGF.
authors.affiliation: Howard Hughes Medical Institute, Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104.
authors.forename: S M
authors.initials: SM
authors.lastname: Thomas
authors.forename: M
authors.initials: M
authors.lastname: Hayes
authors.forename: G
authors.initials: G
authors.lastname: D'Arcangelo
authors.forename: R C
authors.initials: RC
authors.lastname: Armstrong
authors.forename: B E
authors.initials: BE
authors.lastname: Meyer
authors.forename: A
authors.initials: A
authors.lastname: Zilberstein
authors.forename: J S
authors.initials: JS
authors.lastname: Brugge
authors.forename: S
authors.initials: S
authors.lastname: Halegoua
mesh_terms: D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D005786:Gene Expression Regulation; D016391:Genes, src; D007700:Kinetics; D009154:Mutation; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D010766:Phosphorylation; D013696:Temperature; D014443:Tyrosine / Q000378:metabolism
chemical_list: D009414:Nerve Growth Factors; D014443:Tyrosine