32P-postlabelling analysis and micronuclei induction in primary Chinese hamster lung cells exposed to tobacco particulate matter.
The genotoxicity of tobacco particulate matter (TPM) derived from a low-tar, low-nicotine cigarette has been examined by measuring micronucleus induction in a primary pulmonary cell line, both in the absence and presence of an exogenous source of metabolic activation. In an attempt to correlate the cytogenetic damage observed with DNA adduct formation, DNA extracted from TPM-treated cells has been analysed with two different modifications of the 32P-postlabelling assay. The results from the 32P-postlabelling analysis taken together with the pattern of micronucleus induction provide strong evidence that bioreactivated aromatic carcinogens, such as benzo[a]pyrene, are unlikely to be responsible for the TPM-induced cytogenetic damage observed in cultured mammalian cells.
Jones
N J
NJ
Molecular Biology Research Group, School of Biological Sciences, University College of Swansea, UK.
Kadhim
M A
MA
Hoskins
P L
PL
Parry
J M
JM
Waters
R
R
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000187:drug effects*; D008168:Lung / Q000187:drug effects*; D015162:Micronucleus Tests; D010761:Phosphorus Radioisotopes; D012907:Smoking / Q000009:adverse effects*
D010761:Phosphorus Radioisotopes; D001564:Benzo(a)pyrene; D004247:DNA