title: Inhibition of IL-1 beta expression in THP-1 cells by probucol and tocopherol.
abstract: The cytokine interleukin-1, IL-1, likely plays an important role in the early stages of atherogenesis. The possible action of probucol and tocopherol on the expression and secretion of IL-1 beta was investigated using the human monocytic leukemia cell line, THP-1. Both probucol and D-alpha-tocopherol inhibit the phorbol ester-induced release of IL-1 beta without altering differentiation. Analysis of IL-1 beta mRNA levels revealed that probucol and tocopherol had an inhibitory effect on the activation of expression of the IL-1 beta gene. The data suggest that the beneficial effects of probucol may be related to inhibition of IL-1 at an early phase of atherosclerotic plaque formation.
authors.affiliation: Merrell Dow Research Institute, Cincinnati, OH 45215.
authors.forename: A L
authors.initials: AL
authors.lastname: Akeson
authors.forename: C W
authors.initials: CW
authors.lastname: Woods
authors.forename: L B
authors.initials: LB
authors.lastname: Mosher
authors.forename: C E
authors.initials: CE
authors.lastname: Thomas
authors.forename: R L
authors.initials: RL
authors.lastname: Jackson
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism*; D011341:Probucol / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D014810:Vitamin E; D013755:Tetradecanoylphorbol Acetate; D011341:Probucol