Role of the C-terminus in the biological activity of human interleukin 5.
Alterations of the C-terminal amino acid sequence of recombinant human interleukin 5 (rhIL-5) caused significant changes in its biological activity. Removal of eight amino acids from the C-terminus of rhIL-5 by CNBr treatment led to a complete loss of biological activity as determined by the BCL1 cell IgM-inducing assay. Oxidation of Met residue located at C-terminus also resulted in a loss of activity. These results suggest that the C-terminal amino acids of rhIL-5 are crucial for its biological actions.
Kodama
S
S
Suntory Institute for Biomedical Research, Osaka, Japan.
Tsuruoka
N
N
Tsujimoto
M
M
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002700:Chloramines; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis; D007202:Indicators and Reagents; D015848:Interleukin-5 / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000302:isolation & purification / Q000494:pharmacology; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D014105:Tosyl Compounds; D014162:Transfection
D002700:Chloramines; D007075:Immunoglobulin M; D007202:Indicators and Reagents; D015848:Interleukin-5; D010446:Peptide Fragments; D011994:Recombinant Proteins; D014105:Tosyl Compounds; C016300:chloramine-T; D003488:Cyanogen Bromide