title: Functional equivalence of cryptococcal and haptene-specific T suppressor factor (TsF). I. Picryl and oxazolone-specific TsF, which inhibit transfer of contact sensitivity, also inhibit phagocytosis by a subset of macrophages.
abstract: Monoclonal and conventional cryptococcal-specific T suppressor factors (TsF) (also called TsFmp) depress phagocytosis by a subset of macrophages, while picryl- and oxazolone-specific TsF depress the passive transfer of contact sensitivity. This paper shows that these haptene-specific TsF also inhibit phagocytosis by a subset of macrophages and, using this assay, that the anti-haptene TsF resemble the anti-cryptococcal TsF in five respects: (i) the need for reexposure to specific antigen to trigger the release of TsF; (ii) genetic restriction in action; (iii) possession of an antigen-binding site; (iv) expression of I-J determinants; and (v) inactivation by reduction and alkylation. Purification of the anti-picryl TsF by sequential affinity chromatography indicates that the inhibition of phagocytosis is due to the TsF itself and not to a TsF-antigen complex. The TsF inhibits phagocytosis by a direct action as macrophages treated with TsF and exposed to antigen do not release a second factor which inhibits phagocytosis. These results and those of the accompanying paper indicate that the anti-cryptococcal and anti-haptene TsF are functionally equivalent, antigen-specific suppressor factors.
authors.affiliation: Division of Immunological Medicine, Clinical Research Centre, Harrow, Great Britain.
authors.forename: R
authors.initials: R
authors.lastname: Blackstock
authors.forename: M
authors.initials: M
authors.lastname: Zembala
authors.forename: G L
authors.initials: GL
authors.lastname: Asherson
mesh_terms: D000478:Alkylation; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology* / Q000378:metabolism; D003454:Cryptococcus / Q000276:immunology*; D003877:Dermatitis, Contact / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology; D006241:Haptens; D007116:Immunization, Passive; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D010081:Oxazolone / Q000276:immunology*; D010084:Oxidation-Reduction; D010587:Phagocytosis; D010851:Picrates / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology; D013491:Suppressor Factors, Immunologic / Q000737:chemistry / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D006183:H-2 Antigens; D006241:Haptens; D010851:Picrates; D011135:Polysaccharides, Bacterial; D013491:Suppressor Factors, Immunologic; D010081:Oxazolone; C005858:picric acid