Determination of local anaesthetics in body fluids by gas chromatography with surface ionization detection.
Ten local anaesthetics were tested for their detection by gas chromatography (GC)-surface ionization detection (SID). Lidocaine, mepivacaine and bupivacaine were detected with the highest sensitivity; their detection limit was 5-10 pg in an injected volume. The sensitivity of other drugs, such as procaine, dibucaine tetracaine and oxybuprocaine, was an order of magnitude lower than that of the above three local anaesthetics. A detailed procedure for isolation of local anaesthetics from human whole blood and cerebrospinal fluid (CSF) by the use of Sep-Pak C18 cartridges, before the GC-SID, is also presented. The recovery of lidocaine, mepivacaine and bupivacaine, which had been added to 1 ml of whole blood or CSF, was close to 100%.
Hattori
H
H
Department of Legal Medicine, Aichi Medical University, Japan.
Yamamoto
S
S
Yamada
T
T
Suzuki
O
O
D000779:Anesthetics, Local / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D001826:Body Fluids / Q000737:chemistry*; D002045:Bupivacaine / Q000097:blood / Q000134:cerebrospinal fluid; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D008012:Lidocaine / Q000097:blood / Q000134:cerebrospinal fluid; D008619:Mepivacaine / Q000097:blood / Q000134:cerebrospinal fluid
D000779:Anesthetics, Local; D008012:Lidocaine; D008619:Mepivacaine; D002045:Bupivacaine