title: Isolation and characterization of zinc coproporphyrin I: a major fluorescent component in meconium.
abstract: We isolated a substance from an organic extract of meconium (in neutral pH) that exhibited a porphyrin-like fluorescence peak at 580 nm on excitation at 405 nm and deduced its structure. Spectrometric data suggest that the substance is not free coproporphyrin I or free coproporphyrin III, but is a chelate of coproporphyrin I with Zn2+. We also detected a chelate of coproporphyrin III with Zn2+ by HPLC. These substances may be useful as new indicators of the presence of meconium.
authors.affiliation: Department of Chemistry, Hamamatsu University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Horiuchi
authors.forename: K
authors.initials: K
authors.lastname: Adachi
authors.forename: Y
authors.initials: Y
authors.lastname: Fujise
authors.forename: H
authors.initials: H
authors.lastname: Naruse
authors.forename: K
authors.initials: K
authors.lastname: Sumimoto
authors.forename: N
authors.initials: N
authors.lastname: Kanayama
authors.forename: T
authors.initials: T
authors.lastname: Terao
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003306:Coproporphyrins / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008470:Meconium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013054:Spectrophotometry, Atomic; D015032:Zinc / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D003306:Coproporphyrins; C026971:coproporphyrin III; C031467:coproporphyrin I; D015032:Zinc