title: Comparative biochemistry of mammalian arylsulfatase C and steroid sulfatase.
abstract: 1. Hepatic arylsulfatase C (ASC) and steroid sulfatase (SS) from six of eleven mammals (rat, dog, baboon, cow, goat, and sheep) coeluted from DEAE-Sephacel as a single anionic species. A minor cationic peak of ASC and SS activity was also recovered from solubilized microsomes derived from the domestic cat. Characterization of the cationic activities indicated they were most likely contributed by a protein structurally related to the anionic isozyme. Properties of ASC and SS activities occurring in these seven species were most consistent with the presence of both activities in the same enzyme. 2. Guinea-pig liver SS activity was partitioned between an alkylsulfatase (hydrolyzing dehydroepiandrosterone sulfate (DHEAS)) and an arylsulfatase (hydrolyzing both estrone sulfate (E1S) and 4-methylumbelliferyl sulfate (4MUS) at a common active site). These enzymes were physically separable by ion-exchange chromatography and possessed distinct immunological and chemical properties. 3. Porcine, squirrel, and human livers possessed a major isozyme of ASC that lacked both E1S- and DHEAS-sulfatase activities. The human hepatic ASC was separable from SS by electrophoresis and was partially resolved from SS by DEAE-Sephacel chromatography. The ASC isozyme lacking SS activity was heat-labile in all three species.
authors.affiliation: Department of Cell and Structural Biology, University of Illinois, Urbana 61801.
authors.forename: B M
authors.initials: BM
authors.lastname: Ruoff
authors.forename: W L
authors.initials: WL
authors.lastname: Daniel
mesh_terms: D000818:Animals; D001188:Artiodactyla; D001192:Arylsulfatases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D002415:Cats; D002845:Chromatography; D004285:Dogs; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007163:Immunosorbent Techniques; D008099:Liver / Q000201:enzymology*; D010215:Papio; D011489:Protein Denaturation; D012377:Rodentia; D013045:Species Specificity; D043266:Steryl-Sulfatase
chemical_list: D001192:Arylsulfatases; D043266:Steryl-Sulfatase