[The relationship of the saturation density of multilayer cell cultures to their mass exchange with the medium].
Chinese hamster fibroblasts (CHF) and NIH 3T3 cells were cultured on a glass substrate at different distances from the porous membrane separating the cells from the perfusing medium. It is shown that with perfusion of medium above the membrane there is no movement of the medium near the cells. In both the types of culture, the cells grow in multilayers, however the multilayer character of growth in CHF is more pronounced than in NIH 3T3 cells. The saturation density of the cultures depends on the cell-membrane separation, and at separations of no more than 0.2 mm exceeds the saturation density in the monolayer by 8-10 fold. The dependences of the saturation density on separation are different for CHE and NIH 3T3 cells, indicating qualitative differences in the inhibition of cell growth in monolayers between these cultures. The results obtained indicate that the inhibition of cell growth in monolayer is due to mass exchange limitations, rather than to intercellular contact interactions.
Akatov
V S
VS
Lavrovskaia
V P
VP
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media / Q000493:pharmacokinetics*; D003584:Cytological Techniques / Q000295:instrumentation; D005347:Fibroblasts / Q000378:metabolism*; D008433:Mathematics; D008567:Membranes, Artificial; D010477:Perfusion / Q000295:instrumentation; D010637:Phenolsulfonphthalein; D051381:Rats
D003470:Culture Media; D008567:Membranes, Artificial; D010637:Phenolsulfonphthalein
Zavisimost' plotnosti nasyshcheniia mul'tisloĭnykh kul'tur kletok ot ikh massoobmena so sredoĭ.