title: Molecular cloning of the ecotin gene in Escherichia coli.
abstract: The nucleotide sequence of a 876 bp region in E. coli chromosome that encodes Ecotin was determined. The proposed coding sequence for Ecotin is 486 nucleotides long, which would encode a protein consisting of 162 amino acids with a calculated molecular weight of 18,192 Da. The deduced primary sequence of Ecotin includes a 20-residue signal sequence, cleavage of which would give rise to a mature protein with a molecular weight of 16,099 Da. Ecotin does not contain any consensus reactive site sequences of known serine protease inhibitor families, suggesting that Ecotin is a novel inhibitor.
authors.affiliation: Department of Molecular Biology, College of National Sciences, Seoul National University, Korea.
authors.forename: H R
authors.initials: HR
authors.lastname: Lee
authors.forename: J H
authors.initials: JH
authors.lastname: Seo
authors.forename: O M
authors.initials: OM
authors.lastname: Kim
authors.forename: C S
authors.initials: CS
authors.lastname: Lee
authors.forename: S W
authors.initials: SW
authors.lastname: Suh
authors.forename: Y M
authors.initials: YM
authors.lastname: Hong
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: A
authors.initials: A
authors.lastname: Ichihara
authors.forename: D B
authors.initials: DB
authors.lastname: Ha
authors.forename: C H
authors.initials: CH
authors.lastname: Chung
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D033903:Periplasmic Proteins; D021382:Protein Sorting Signals / Q000737:chemistry; D015183:Restriction Mapping
chemical_list: D001426:Bacterial Proteins; C039076:Eco protein, E coli; D029968:Escherichia coli Proteins; D033903:Periplasmic Proteins; D021382:Protein Sorting Signals