Analysis of 2-amino-N6-hydroxyadenine-induced mutagenesis in phage M13mp2.
The mechanism of mutagenesis induced by 2-amino-N6-hydroxyadenine (AHA) and its deoxyriboside (AHAdR) was studied by determining the nucleotide sequences of phage M13mp2 mutant DNA samples. Mutations in the lac promoter-lacZ alpha region of the phage were induced by addition of this agent to culture media in which the phage was growing inside the host bacteria. The spectrum of spontaneous mutation was also investigated. The induced sequence changes were mostly base transitions (80% with AHA and 90% with AHAdR). A few single-base deletions and additions were detected, but they were ascribable to spontaneous mutations. These results are consistent with the incorporation type mechanism proposed by Janion (this issue). In the Ames Salmonella assay, both AHA and AHAdR showed strong mutagenicity in strain TA100 but no activity in TA98.
Tsuchiyama
H
H
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Atsumi
G
G
Matsuda
A
A
Negishi
K
K
Hayatsu
H
H
D000225:Adenine / Q000031:analogs & derivatives* / Q000633:toxicity; D001483:Base Sequence; D003090:Coliphages / Q000187:drug effects* / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D007763:Lac Operon; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009152:Mutagenicity Tests; D009153:Mutagens; D011401:Promoter Regions, Genetic; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
D004269:DNA, Bacterial; D004279:DNA, Viral; D009153:Mutagens; C030632:2-amino-N(6)-hydroxyadenine; D000225:Adenine