[The significance of immunoblot in serodiagnosis of cysticercosis cellulosae].
50, 92 and 30 samples collected respectively from cases with cysticercosis cellulosae, other parasitic diseases and normal controls were examined with SDS-gradient (5-20%) polyacrylamide-gel electrophoresis and immunoblot. The results showed that 26 KD protein band of the cysticercus antigen is highly specific for cysticercosis and no cross reaction could be seen in patients with other parasitic diseases, especially hydatid disease, and healthy controls. As we know that the cysticercus antigen usually has a high cross-reaction rate with hydatidosis by ELISA method, the positive rate of this test for cysticercosis is 70% in comparison with 80% and 76% of ELISA and CFT respectively and the difference in the figures of these three tests has no statistical significance. Our study suggested that this method is valuable in clinical diagnosis and epidemiological surveys for research work on cysticercosis cellulosae.
Liu
D
D
Beijing Tropical Medicine Research Institute.
Xu
Z B
ZB
Hu
Y X
YX
D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D015153:Blotting, Western; D003551:Cysticercosis / Q000175:diagnosis*; D003552:Cysticercus / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans
D000947:Antigens, Helminth