An enzyme-linked immunosorbent assay (ELISA) for the detection and quantitation of the tumor marker 1-methylinosine in human urine.
A highly sensitive enzyme-linked immunoassay (ELISA) was developed to detect and quantify the tumor marker, 1-methylinosine (m1I), in human urine. The rabbit antisera was highly specific for m1I with negligible or no inhibition by other nucleosides excreted into urine. Using the competitive ELISA, nanogram amounts of m1I were easily measured directly in urine. The assay agreed with our previous hplc analysis of m1I in urine for identifying those individuals with chronic myelogenous leukemia. Thus, this assay should greatly facilitate the quantitation of m1I as a tumor marker.
D'Ambrosio
S M
SM
Division of Radiobiology, Ohio State University, Columbus 43210.
Gibson-D'Ambrosio
R E
RE
Trewyn
R W
RW
D000918:Antibody Specificity; D014408:Biomarkers, Tumor / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007288:Inosine / Q000031:analogs & derivatives* / Q000276:immunology / Q000652:urine; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000652:urine; D008832:Microchemistry
D014408:Biomarkers, Tumor; D007106:Immune Sera; C012433:1-methylinosine; D007288:Inosine