title: Intrathecal immunoglobulin synthesis. Comparison of various formulae with the 'gold standard' of isoelectric focusing.
abstract: The recent introduction of a further new formula for calculating intrathecal Ig synthesis led us to compare the results of calculations obtained when using it and certain other older formulae with those of isoelectric focusing (IEF), since only the latter proves the intrathecal synthesis of oligoclonal proteins. On comparison, it was found that the results produced by the usual formulae were, without exception, inferior to those of isoelectric focusing. By using Receiver Operating Characteristic (ROC) curves, it is shown that calculations with the Reiber formula agree best with IEF. Virtually the same results are obtained with the "ln IgG index + 1" and "IgG index".
authors.affiliation: Department of Clinical Chemistry, University Hospital Leiden, The Netherlands.
authors.forename: J H
authors.initials: JH
authors.lastname: Souverijn
authors.forename: H M
authors.initials: HM
authors.lastname: Serrée
authors.forename: R
authors.initials: R
authors.lastname: Peet
authors.forename: W
authors.initials: W
authors.lastname: Grenzebach Smit
authors.forename: G W
authors.initials: GW
authors.lastname: Bruyn
mesh_terms: D000465:Algorithms; D000917:Antibody Formation; D001769:Blood / Q000276:immunology; D005189:False Positive Reactions; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid; D007136:Immunoglobulins / Q000096:biosynthesis / Q000134:cerebrospinal fluid*; D007525:Isoelectric Focusing; D012372:ROC Curve
chemical_list: D007074:Immunoglobulin G; D007136:Immunoglobulins