DNA strand break metabolism in human lymphocytes: a reevaluation.
Recent findings concerning the presumed existence of single-strand breaks (SSB) in quiescent human peripheral blood lymphocytes (PBL) are discussed in relation to the role of poly(ADP-ribosyl)ation in DNA strand break metabolism. It is argued that the activation of poly(ADP-ribose)polymerase (ADPRP) by a DNA-damaging agent is not indicative of an obligatory role of poly(ADP-ribosyl)ation in DNA repair. From this it follows that SSB induced by different strand-breaking agents might be removed by either ADPRP-dependent or ADPRP-independent DNA repair pathways.
Boerrigter
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ME
Harvard Medical School, Beth Israel Hospital, Gerontology Division, Boston, Massachusetts 02215.
D004249:DNA Damage; D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*
D004277:DNA, Single-Stranded