Determination of adriamycin in plasma and tissue biopsies.
A simple, rapid and sensitive method for the extraction and high-performance liquid chromatographic analysis of adriamycin in tissue and plasma is described. Tissue (5-100 mg) and plasma (1 ml) samples underwent a C18 Sep-Pak extraction into methanol. Chromatography was performed on a muBondapakphenyl column using a mobile phase of acetonitrile-0.1 M ammonium formate (pH 4.0) with a flow-rate of 2 ml/min. Fluorometric detection was used with an excitation of 480 nm and an emission of 550 nm. The procedure produced a linear curve for the concentration range 25-1000 ng/ml. The development of the assay produced rapid, repeatable and accurate results for both small tissue samples and plasma.
Cox
S K
SK
Department of Environmental Practice, College of Veterinary Medicine, University of Tennessee, Knoxville 37901.
Wilke
A V
AV
Frazier
D
D
D000818:Animals; D001706:Biopsy; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004285:Dogs; D004317:Doxorubicin / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D006801:Humans
D004317:Doxorubicin