Mast cells in the rat gastric mucosa are not primarily responsible for PGD2 generation.
The present study investigates the contribution of gastric mast cells on PGD2 generation in rat gastric mucosa. Cold-restraint induced stress or i.v. carbachol injection methods were used for gastric mast cell degranulation. In 19 stressed, 15 carbachol-infused and 14 control rats, gastric mast cell counts and gastric mucosa PGD2 assay were performed. Gastric mucosal content of PGF2 alpha was also determined in carbachol infused and control rats. The mean number of gastric mast cells was significantly lower in stressed and carbachol infused than in control rats. Despite these differences in gastric mast cell counts, neither PGD2 or PGF2 alpha contents in the gastric mucosa were significantly different in mast cells degranulated rats than in control animals. These results suggest another source of PGD2 in the rat gastric mucosa other than mast cells.
Bulbena
O
O
Department of Pharmacology, Lab. Vinas SA, Barcelona, Spain.
Masana
N
N
Ramis
I
I
Abdalla
S
S
Perez
R
R
Pique
J M
JM
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D015550:Cell Degranulation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D015237:Dinoprost / Q000032:analysis / Q000378:metabolism*; D005260:Female; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D015230:Prostaglandin D2 / Q000032:analysis / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D002217:Carbachol; D015237:Dinoprost; D015230:Prostaglandin D2; D007213:Indomethacin