Determination of xanthenone-4-acetic acid in mouse plasma by high-performance liquid chromatography.
Xanthenone-4-acetic acid (XAA) was synthesised during a search for improved analogues of flavone-8-acetic acid, an antitumour agent with a unique mechanism of action but with a number of pharmacological disadvantages. We describe a simple, selective high-performance liquid chromatographic assay suitable for the detection of XAA in mouse plasma. After addition of an internal standard (3-methyl-XAA), plasma was acidified with trichloroacetic acid and extracted with toluene. After evaporation of solvent, samples were chromatographed on a C18 4-microns Novapak cartridge (mobile phase: water-acetonitrile-acetic acid, 65:35:2, v/v) using fluorescence detection. At the maximum tolerated dose of XAA (725 mumol/kg), nonlinear pharmacokinetics were observed.
Kestell
P
P
Cancer Research Laboratory, University of Auckland Medical School, New Zealand.
McKeage
M J
MJ
Baguley
B C
BC
D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D003110:Colonic Neoplasms / Q000097:blood; D008297:Male; D051379:Mice; D014966:Xanthenes / Q000097:blood* / Q000493:pharmacokinetics
D000970:Antineoplastic Agents; D014966:Xanthenes; C066138:xanthenone-4-acetic acid