[The heterogeneity of collection strains of Yersinia pseudotuberculosis and their molecular biological properties].
The capacity of Y. pseudotuberculosis strains for disassociation with the appearance of S- and P-forms has been studied. Strains 852 and 9547 show high stability in S-forms, their conversion into R-forms occurring at 40-42 degrees C. Strain 6953 shows pronounced polymorphism and instability of its associations at different growth temperatures. Strain 9532 exists in S- and R-forms which retain their stability during numerous subculturings at different growth temperatures and prolonged storage. This strain has plasmids of 130, 72.2, 5.7 kb. All plasmids are retained in S- and R-forms, i. e. the dissociation of the strain is not accompanied by the loss of plasmids. The conversion of the strain from the S-form into the R-form leads to changes in the structure of lipopolysaccharide and the composition of low-molecular (less than 23 kD) proteins in the outer and inner membranes. In tests on guinea pigs the LD50 of the R-form of the strain is tenfold greater than that of its S-form. The dissociants of strain 9532 are transformed by plasmid DNA with equal efficiency and equally inherit them without selective pressure.
Shmelev
V A
VA
Nosova
L Iu
LIu
Galeev
V S
VS
Stavitskiĭ
S B
SB
Lushchikov
S B
SB
Popov
S G
SG
Gerasimov
V N
VN
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000302:isolation & purification; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D006168:Guinea Pigs; D008070:Lipopolysaccharides / Q000032:analysis / Q000302:isolation & purification; D008854:Microscopy, Electron; D010957:Plasmids / Q000235:genetics; D014169:Transformation, Bacterial / Q000235:genetics; D014774:Virulence / Q000187:drug effects; D015011:Yersinia pseudotuberculosis / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity / Q000648:ultrastructure
D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D008070:Lipopolysaccharides
Geterogennost' muzeĭnykh shtammov Yersinia pseudotuberculosis i ikh molekuliarno-biologicheskie svoĭstva.