[Transcortin in rat kidney tissue: distribution in microsomal fractions].
During chromatography of renal tissue cytosolic proteins on DEAE-cellulose the protein specifically binding [3H]corticosterone is eluted within the potassium phosphate concentration range of 0.08-0.10 M. Analysis of kidney slices revealed the synthesis of [3H]transcortin whose electrophoretic mobility was close to that of the blood plasma protein. Using radioimmunochemical methods, it has been found that transcortin-specific [125I]IgG antibodies interact with growing polypeptide chains of membrane-bound polyribosomes. Free polyribosomes do not bind antibodies against transcortin.
Krauialis
K I
KI
Ulinskaĭte
A P
AP
Meĭlus
V I
VI
D000818:Animals; D000906:Antibodies; D002848:Chromatography, DEAE-Cellulose; D003345:Corticosterone / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008861:Microsomes / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D014156:Transcortin / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D014156:Transcortin; D003345:Corticosterone
Transkortin v tkaniakh pochek krys: raspredelenie v mikrosomal'noĭ fraktsii.