Liquid chromatographic determination of chloramphenicol in calf tissues: studies of stability in muscle, kidney, and liver.
A liquid chromatographic method has been developed for the measurement of chloramphenicol (CAP) in muscle, liver, and kidney. The mean recovery levels were 82.6, 75.3, and 79.2% in muscle, liver, and kidney, respectively. The method was repeatable and reproducible for CAP measurement in muscle, with a detection limit of 1 microgram/kg. Investigation of CAP stability in muscle, liver, and kidney showed that CAP stability in muscle was good at -20 degrees C; for spiked liver and kidney, degradation of CAP was observed, and the use of piperonyl butoxide (PB) for metabolism inhibition was recommended for recovery and linearity studies. However, PB was unnecessary for preservation of treated animal tissues if samples were cut into cubes and cooled at -20 degrees C, just after slaughter, pending analysis. With these limitations, CAP can be measured in liver and kidney.
Sanders
P
P
Laboratoire des Medicaments Vétérinaires, Centre National d'Etudes Vétérinaires et Alimentaires, Ministère de l'Agriculture et de la Forêt, La Haute Marche-Javené, Fougères.
Guillot
P
P
Dagorn
M
M
Delmas
J M
JM
D000818:Animals; D002417:Cattle; D002701:Chloramphenicol / Q000032:analysis*; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis*; D007202:Indicators and Reagents; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D010882:Piperonyl Butoxide / Q000737:chemistry; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D007202:Indicators and Reagents; D012997:Solvents; D002701:Chloramphenicol; D010882:Piperonyl Butoxide