Segmental vascular resistance during pulsatile and steady perfusion in 3- to 5-wk-old rabbit lungs.
To determine the mechanisms by which pulsatile perfusion reduces vascular resistance in isolated lungs, we compared the effects of pulsatile versus steady perfusion on total and segmental vascular resistance in isolated lungs of 3- to 5-wk-old rabbits. Lungs were perfused alternately with steady and pulsatile flow for 45-min periods at a constant total arteriovenous pressure drop. Blood flow was adjusted to keep mean pulmonary arterial pressure at 20 cmH2O, when left atrial and airway pressures were 8 and 6 cmH2O, respectively. We partitioned the pulmonary circulation into three longitudinal vascular segments, i.e., arteries, microvessels, and veins, by measuring pressures in 20- to 50-microns-diameter subpleural arterioles and venules by the micropipette servo-nulling method. We found that in the isolated, perfused 3- to 5-wk-old rabbit lung, in which arteries and veins are the main sites of resistance, pulsatile flow results in a 20-36% reduction in total vascular resistance, mainly due to a reduction in arterial and venous resistances. The decrease in total vascular resistance was similar in lungs that were untreated or treated with papaverine, indicating that the effect of pulsatile flow was not due to active vasomotion. The reduction in arterial resistance was greater than that in veins (31-55 vs. 19-22%), especially when pulse amplitude was high (5-10 vs. 20-30 cmH2O). Total vascular resistance was also lower after 45 min of pulsatile perfusion with a pulse rate of 200 pulses/min than 80 pulses/min (0.126 +/- 0.04 vs. 0.154 +/- 0.059 cmH2O.min.ml-1.kg).(ABSTRACT TRUNCATED AT 250 WORDS)
Kaapa
P
P
Department of Pediatrics, Harbor-University of California Los Angeles Medical Center, Torrance 90509.
Usha Raj
J
J
Hillyard
R
R
Anderson
J
J
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D066298:In Vitro Techniques; D010208:Papaverine / Q000494:pharmacology; D010477:Perfusion; D011312:Pressure; D011652:Pulmonary Circulation / Q000187:drug effects; D011673:Pulsatile Flow; D011817:Rabbits; D014655:Vascular Resistance; D014666:Vasomotor System / Q000502:physiology
D010208:Papaverine