Multiple quantum NMR spectroscopy methods for measuring the apparent self-diffusion coefficient of in vivo lactic acid.
Nuclear magnetic resonance spectroscopy methods are presented for measuring the apparent self-diffusion coefficient of lactic acid in vivo. These techniques incorporate diffusion-sensitive gradients into multiple quantum NMR pulse sequences that are employed for spectral editing. Both diffusion-sensitive zero quantum (ZQ) and double quantum (DQ) spectral editing techniques have been developed and the DQ method has been used to measure the apparent self-diffusion coefficient of in vivo lactic acid in H-MESO-1 human tumor xenografts subcutaneously implanted in athymic mice.
Sotak
C H
CH
Department of Biomedical Engineering, Worcester Polytechnic Institute, MA 01609.
D004058:Diffusion; D007773:Lactates / Q000737:chemistry*; D019344:Lactic Acid; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008956:Models, Chemical
D007773:Lactates; D019344:Lactic Acid