RPC19, the gene for a subunit common to yeast RNA polymerases A (I) and C (III).
Yeast RNA polymerases A (I) and C (III) share a subunit called AC19. The gene encoding AC19 has been isolated from yeast genomic DNA using oligonucleotide probes deduced from peptide sequences of the isolated subunit. This gene (RPC19) contains an intron-free open reading frame of 143 amino acid residues. RPC19 is a single copy gene that maps on chromosome II and is essential for cell viability. The amino acid sequence contains a sequence motif common to the Escherichia coli RNA polymerase alpha subunit, the Saccharomyces cerevisiae AC40 and B44.5 subunits, the human hRPB33 product, and the CnjC conjugation-specific gene product of Tetrahymena. The 5'-upstream region contains a sequence element, the PAC box, that has been conserved in at least 10 genes encoding subunits of RNA polymerases A and C.
Dequard-Chablat
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M
Departement de Biologie Cellulaire et Moléculaire-Service de Biochimie et de Génétique Moléculaire, Centre d'Etudes Nucléaires de Saclay, Gif-sur-Yvette, France.
Riva
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Carles
C
C
Sentenac
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D004271:DNA, Fungal / Q000032:analysis; D004926:Escherichia coli; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D012318:RNA Polymerase I / Q000235:genetics*; D012320:RNA Polymerase III / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013768:Tetrahymena
D004271:DNA, Fungal; D012318:RNA Polymerase I; D012320:RNA Polymerase III