Cataract induction by 1,2-naphthoquinone. I. Studies on the redox properties of bovine lens proteins.
Conditions of oxidative stress may lead to cataract formation. Reaction of certain flavoproteins, the NADH: oxidoreductases, with different quinones is well known to form hydrogen-peroxide. This reaction was investigated to get more information on cataract induction by naphthalene and its quinone metabolites. Protein extracts from bovine lens cortex exhibit "diaphorase" activity, indicated as dye reduction in the presence of NADH and dichlorophenol-indophenol (DCPIP) or ferricyanide. Different redox cycling compounds are shown to be active in this "diaphorase" reaction by lens protein extract (LCE): Oxygen consumption can be detected in the presence of pyrroloquinoline quinone and juglone whereas 1,4-naphthoquinone, menadione and paraquat are no redox cyclists in this flavoprotein catalyzed reaction.
Kleber
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Institut für Botanik und Mikrobiologie, Technische Universität München, Bundesrepublik Deutschland.
Kröner
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Elstner
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EF
D000818:Animals; D002386:Cataract / Q000139:chemically induced*; D002417:Cattle; D003459:Crystallins / Q000378:metabolism*; D007908:Lens, Crystalline / Q000187:drug effects / Q000378:metabolism*; D009285:Naphthoquinones / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects*; D011808:Quinone Reductases / Q000378:metabolism; D011809:Quinones / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D003459:Crystallins; D009285:Naphthoquinones; D011809:Quinones; C040756:1,2-naphthoquinone; D011808:Quinone Reductases