Host passage-dependent wheat germ agglutinin-binding proteins of Chlamydia trachomatis.
Wheat germ agglutinin (WGA)-binding proteins in the highly purified elementary bodies (EB) of Chlamydia trachomatis were detected by an ELISA-like assay of immobilized EB. Trypsin-sensitivity of the WGA-binding moieties was detected only in the chlamydiae grown in HeLa cells. A nonionic detergent, beta-octyl-D-glucopyranoside (OG), was used to extract proteins from the purified EB. Proteins in the extract were resolved by SDS-PAGE and probed with 125I-labelled WGA. Several proteins reacted with WGA in a specific manner. Some of these WGA-binding proteins in the EB of serovars L1 and E exhibited altered molecular mass after adaptation in the two alternate hosts, HeLa and McCoy cells. These results suggest that WGA-binding proteins exist in chlamydiae.
Goswami
P C
PC
Department of Microbiology, St. Louis University School of Medicine, MO 63104-1028.
Vretou
E
E
Bose
S K
SK
D002352:Carrier Proteins / Q000032:analysis*; D002692:Chlamydia trachomatis / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D014909:Wheat Germ Agglutinins / Q000378:metabolism*
D002352:Carrier Proteins; D014909:Wheat Germ Agglutinins