Rapid increase in LHRH mRNA levels following NMDA.
To test the hypothesis that changes in LHRH mRNA levels are tightly linked to changes in LHRH secretion, intact male rats were infused with NMDA, a substance which increases LH release by a hypothalamic action. NMDA significantly elevated cellular levels of LHRH mRNA at 15 and 60 min. Similar changes in LH levels were induced by NMDA. These effects of NMDA on LHRH mRNA levels were not due to a generalized excitatory effect in the brain because levels of mRNAs encoding proopiomelanocortin (POMC) and tyrosine hydroxylase (TH) in the arcuate-periarcuate region were not altered by NMDA and levels of TH mRNA were decreased significantly at 15 and 60 min in neurons of the zona incerta. These data strongly support the hypothesis that changes in cellular levels of LHRH mRNA are tightly linked temporally to changes in LHRH secretion.
Petersen
S L
SL
University of Missouri-Columbia, School of Medicine, Columbia 65212.
McCrone
S
S
Keller
M
M
Gardner
E
E
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000187:drug effects / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics*; D007031:Hypothalamus / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008297:Male; D016202:N-Methylaspartate / Q000494:pharmacology*; D015345:Oligonucleotide Probes; D011333:Pro-Opiomelanocortin / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D007987:Gonadotropin-Releasing Hormone; D016202:N-Methylaspartate; D011333:Pro-Opiomelanocortin