[Use of a temporary protective group during the synthesis of oligonucleotides with an unnatural bond].
Methoxygroup has been used for transient P-protection on H-phosphonate oligonucleotide synthesis. Whereas other H-phosphonate linkages can be irreversibly transformed into phosphoramidates by treatment with alkylamines, the protected phosphoralkoxyl groups generate natural phosphodiester linkages after the final deprotection. The method allows for synthesis of oligonucleotides with addressed position of internucleotide phosphoralkylamidate groups.
Samoshin
V V
VV
Kudiakov
Iu E
IuE
Smirnov
V D
VD
D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds / Q000737:chemistry
D009841:Oligonucleotides; D009943:Organophosphorus Compounds
Ispol'zovanie vremennoĭ zashchitnoĭnteze oligonukleotid ov s neprirodnoĭ sviaz'iu.