title: A method for enzyme- and immunohistochemical staining of large frozen specimens.
abstract: A method for large specimen cryosectioning is described. Specimens of pig heart ventricles were lightly fixed by microwave irradiation, embedded in 10% gelatin, frozen in hexan chilled with dry ice, and sectioned using an LKB 2250 PMW cryomicrotome. The sections were collected on transparent film and transferred onto glass slides. Standard histological, enzyme- and immuno-histochemical staining techniques were used. The present method allowed cryosectioning with sections of good quality which could be used for enzyme- and immuno-staining. For studies of experimental myocardial infarction, staining for phosphorylase and Periodic Acid Schiff outlined the ischemic area and antibodies against plasma fibronectin and fibrinogen delineated the infarcted myocardium.
authors.affiliation: Department of Anatomy, University of Umeå, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Holmbom
authors.forename: M
authors.initials: M
authors.lastname: Lindström
authors.forename: U
authors.initials: U
authors.lastname: Näslund
authors.forename: L E
authors.initials: LE
authors.lastname: Thornell
mesh_terms: D000818:Animals; D005340:Fibrinogen / Q000032:analysis; D005353:Fibronectins / Q000032:analysis; D005629:Frozen Sections; D005780:Gelatin; D006651:Histocytochemistry / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice; D008872:Microwaves; D009203:Myocardial Infarction / Q000378:metabolism*; D009206:Myocardium / Q000737:chemistry*; D013552:Swine
chemical_list: D005353:Fibronectins; D005780:Gelatin; D005340:Fibrinogen