Identification of a polypeptide bound to the beta region of the mouse r protein L32 promoter.
Studies have been initiated to identify the protein component(s) which interact with the beta regulatory region of the mouse ribosomal protein L32 gene promoter. By the combined use of the mobility shift assay and UV cross-linking, a factor specific for the upstream transcriptional control sequence of the beta region of the ribosomal protein L32 promoter has been detected in mouse L1210 nuclear extracts. A mutation (GT----TC at -71 to -70) in this sequence eliminates the binding. Beta factor is identified as a 55 kDa polypeptide by UV cross-linking. Addition of excess beta element (double-stranded oligonucleotide) to a cell-free transcription system reduces transcription of the ribosomal protein L32 gene. Our results provide evidence that the interaction between the beta element and the beta factor is involved in ribosomal protein L32 transcription.
Yoganathan
T
T
Department of Molecular Biology and Genetics, College of Biological Science, University of Guelph, Ontario, Canada.
Sells
B H
BH
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D004247:DNA; D004268:DNA-Binding Proteins / Q000032:analysis*; D004586:Electrophoresis; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012269:Ribosomal Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000032:analysis; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D012269:Ribosomal Proteins; D014157:Transcription Factors; C041806:ribosomal protein L32; D004247:DNA