Effect of ethanol on phospholipid turnover and calcium mobilization in chick embryos.
Effects of ethanol on [3H]inositol and [14C]choline incorporation into phosphatidylinositol (PI) and phosphatidylcholine (PC), free intrasynaptosomal Ca2+ ([Ca2+]i) and synaptosomal 45Ca2+ uptake, were investigated in the brain and heart of 17-day-old chick embryos to which a 10% ethanol solution had been injected on the 3rd day of embryogenesis. In brain synaptosomes, ethanol increased the incorporation of [3H]inositol and [14C]choline into PI and PC, increased [Ca2+]i, and decreased 45Ca2+ uptake. On the other hand, in heart synaptosomal membrane, ethanol decreased the incorporation of [3H]inositol and [14C]choline into PI and PC, decreased [Ca2+]i, and increased 45Ca2+ uptake. Ethanol stimulated in vitro [3H]inositol and [14C]choline incorporation into PI and PC in the brain and heart in both the control and ethanol-treated groups. However, addition of ethanol did not affect the release of 45Ca2+ from the synaptosomal membrane of either organ in either group. Addition of ethanol inhibited 45Ca2+ uptake in a dose-dependent manner in the brain but not in the heart. In both organs, there was a relationship between phospholipid turnover and [Ca2+]i after ethanol.
Natsuki
R
R
Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Japan.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000196:embryology; D002118:Calcium / Q000378:metabolism*; D002642:Chick Embryo; D002794:Choline / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects / Q000196:embryology; D007294:Inositol / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis*; D013574:Synaptosomes / Q000187:drug effects
D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D010743:Phospholipids; D000431:Ethanol; D007294:Inositol; D002794:Choline; D002118:Calcium