Study of the identification of enterotoxigenic Escherichia coli by LT-DNA gene hybridization.
Reference strains of enterotoxigenic Escherichia coli (ETEC), non-ETEC, and other enteropathogenic bacteria were used to prove the reliability of LT-DNA gene hybridization. In the test, LT-DNA gene hybridization was compared with plate immunohemolytic test (PIHT) in identifying LT-ETEC. The results obtained by both methods showed no significant differences. 791 strains of E. coli isolated from 1,875 children with acute diarrhea in Taiyuan Children's Hospital were examined for LT-ETEC by LT-DNA gene hybridization and PIHT. 289 strains examined by LT-DNA gene hybridization were LT positive, while 205 strains examined by PIHT were LT positive. Three different assays were done: colony hybridization, PIHT and fecal direct blot hybridization on each of 74 fecal specimens from children with acute diarrhea. It was found that identification of LT-ETEC using fecal direct blot hybridization is a simple, sensitive and more practical method.
Zhu
Q Y
QY
Taiyuan Children's Hospital, Shanxi.
Li
L Q
LQ
Huang
Y T
YT
Lin
W M
WM
Li
Y T
YT
Wang
J
J
D001427:Bacterial Toxins / Q000032:analysis*; D015139:Blotting, Southern; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis*; D003967:Diarrhea / Q000382:microbiology*; D004768:Enterotoxins / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D029968:Escherichia coli Proteins; D006801:Humans; D007223:Infant; D009693:Nucleic Acid Hybridization
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli