The genetic activity of N6-hydroxyadenine and 2-amino-N6-hydroxyadenine in Escherichia coli, Salmonella typhimurium and Saccharomyces cerevisiae.
The genetic activity of 2-amino-N6-hydroxyadenine or 2-amino-N-hydroxylaminopurine (AHA) and N6-hydroxyadenine or 6-N-hydroxylaminopurine (HAP) was studied in S. typhimurium, E. coli and Saccharomyces cerevisiae strains. AHA was a more potent mutagen for bacteria and a less potent mutagen for yeast than HAP. The mutagenic activity of analogs was not influenced by excision, mutagenic or double-strand DNA repair mutations. On the other hand, the uvrBdel mutation has a drastic effect on the mutagenicity and toxicity of both analogs in the Salmonella strains studied. HAP was a very potent mutagen in yeast with a low capability of inducing mitotic recombination contrary to common mutagens, possessed unique intergenic specificity and was able to induce mutations in diploids at rather high frequency.
Pavlov
Y I
YI
Department of Genetics and Breeding, Leningrad State University, U.S.S.R.
Noskov
V N
VN
Lange
E K
EK
Moiseeva
E V
EV
Pshenichnov
M R
MR
Khromov-Borisov
N N
NN
D000225:Adenine / Q000031:analogs & derivatives* / Q000633:toxicity; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D005804:Genes, Lethal; D016296:Mutagenesis; D009152:Mutagenicity Tests; D009153:Mutagens; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics
D009153:Mutagens; C032610:6-N-hydroxylaminopurine; C030632:2-amino-N(6)-hydroxyadenine; D000225:Adenine