title: Measurement of brown adipose tissue uncoupling protein by enzyme linked immunosorbent assay.
abstract: Antibody to uncoupling protein (UCP) purified from rat brown adipose tissue (BAT) was raised in rabbits and an enzyme linked immunosorbent assay was developed. The antiserum did not cross-react with other mitochondrial proteins from BAT and from other tissues but cross-reacted with UCP from hamster, guinea pig and mouse. The assay is capable of detecting 5 ng of UCP. Using this assay and a crude mitochondrial preparation, UCP content of BAT was shown to increase during cold adaptation.
authors.affiliation: Department of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, N.T.
authors.forename: E L
authors.initials: EL
authors.lastname: Chan
authors.forename: R
authors.initials: R
authors.lastname: Swaminathan
mesh_terms: D000222:Adaptation, Physiological; D002001:Adipose Tissue, Brown / Q000737:chemistry*; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins; D003080:Cold Temperature; D006224:Cricetinae; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D007473:Ion Channels; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D051379:Mice; D008928:Mitochondria / Q000737:chemistry*; D008930:Mitochondria, Liver / Q000737:chemistry; D008931:Mitochondria, Muscle / Q000737:chemistry; D024101:Mitochondrial Proteins; D051381:Rats; D011919:Rats, Inbred Strains; D014475:Uncoupling Agents / Q000032:analysis* / Q000276:immunology; D000071256:Uncoupling Protein 1
chemical_list: D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D014475:Uncoupling Agents; D000071256:Uncoupling Protein 1