title: Enterococcus seriolicida sp. nov., a fish pathogen.
abstract: The properties and taxonomic position of bacterial strains isolated from diseased specimens of cultured yellowtail and eels were examined. The isolates were gram-positive, short-chain-forming, catalase-negative, facultatively anaerobic cocci. Growth at 10 and 45 degrees C in 6.5% NaCl (pH 9.6) with 40% bile and in 0.1% methylene blue-milk were both positive. The isolates could be distinguished from other species of the genus Enterococcus by several biochemical characteristics and by Lancefield's group antigen. Guanine-plus-cytosine content of DNA was 44 mol% as determined by the thermal melting temperature. The value for DNA-DNA hybridization was sufficiently low to warrant distinguishing this species from reported. Enterococcus species. The name Enterococcus seriolicida is proposed. The type strain is YT-3 (=ATCC 49156).
authors.affiliation: Fish Disease Laboratory, Faculty of Agriculture, Kochi University, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Kusuda
authors.forename: K
authors.initials: K
authors.lastname: Kawai
authors.forename: F
authors.initials: F
authors.lastname: Salati
authors.forename: C R
authors.initials: CR
authors.lastname: Banner
authors.forename: J L
authors.initials: JL
authors.lastname: Fryer
mesh_terms: D000818:Animals; D004524:Eels / Q000382:microbiology*; D005393:Fish Diseases / Q000382:microbiology; D005399:Fishes / Q000382:microbiology*; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D013291:Streptococcus / Q000145:classification* / Q000302:isolation & purification / Q000648:ultrastructure