[II. Use of antibodies to DNA modified by trans-diaminodichloroplatinum, for identification of specific DNA sequences].
DNA modified by trans diaminedichlorplatinum-II (transDDP) has been suggested as an effective probe for non-isotopic hybridization and high-specific anti-DNA-transDDP antibodies with horse radish peroxidase or alkaline phosphotase conjugated antibodies to rabbit Ig and protein A-peroxidase - for hybrids visualization. This method allows to detect 2 pg/mm DNA.
Kiseleva
V I
VI
Turchinskiĭ
M F
MF
Poverennyĭ
A M
AM
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001483:Base Sequence; D002417:Cattle; D002945:Cisplatin / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects / Q000276:immunology*; D007124:Immunoenzyme Techniques; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D013237:Stereoisomerism; D013950:Thymus Gland / Q000378:metabolism
D000906:Antibodies; C034697:transplatin; D004247:DNA; D002945:Cisplatin
II. Ispol'zovanie antitel k DNK, modifitsirovannoĭ trans-diamindikhlorplatinoĭ, dlia identifikatsii spetsificheskikh posledovatel'nosti DNK.