Splicing pattern of Gs alpha mRNA in human and rat brain.
Gs alpha, the GTP-binding protein that mediates receptor stimulation of adenylyl cyclase, exists in small (Gs alpha-S) and large (Gs alpha-L) molecular forms. Previous work has established that these Gs alpha isoforms are products of alternatively spliced Gs alpha pre-mRNA. The present work examined the splicing pattern of Gs alpha mRNA in rat and human brain using a method based upon the polymerase chain reaction. In rat brain, transcripts encoding Gs alpha-L were most abundant and constituted 92% of total Gs alpha mRNA in hippocampus and cerebral cortex and 84% in striatum. Compared to rat brain, the relative abundance of Gs alpha-L mRNA was less in human brain and constituted 84% of total Gs alpha mRNA in hippocampus and cortex and 66% in striatum. In addition, it was found that approximately 40% of the Gs alpha-S mRNA utilizes an alternative splice site that encodes serine at amino acid 72. No regional variations in this splicing pattern were found.
Granneman
J G
JG
Center for Cell Biology, Sinai Research Institute, Detroit, Michigan.
Bannon
M J
MJ
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism / Q000502:physiology*; D002540:Cerebral Cortex / Q000378:metabolism / Q000502:physiology; D005260:Female; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism / Q000502:physiology; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012326:RNA Splicing / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D014158:Transcription, Genetic / Q000235:genetics / Q000502:physiology
D012333:RNA, Messenger; C034882:guanosine 5'-O-(1-thiotriphosphate); D016244:Guanosine 5'-O-(3-Thiotriphosphate)