Lens autofluorescence in healthy individuals.
We measured blue-green autofluorescence (AF, 495 nm/520 nm) of the lens in 43 random eyes of 43 healthy volunteers aged 6-86 years, five in each decade, using an instrument designed by one of us (HN). The instrument generates an autofluorescence profile, which consists of anterior and posterior juxtacortical peaks and a central plateau. The height of the anterior peak was taken as a maximum autofluorescence value and the square root of the ratio between the posterior and the anterior peak was used as a lens transmission index. The coefficient of variation for the measurement technique was 3.9% for maximum autofluorescence and 2.9% for lens transmission index. Both the maximum autofluorescence and the transmission index were highly correlated with age. Statistically over 90% of the variation in maximum autofluorescence values and almost 70% of the variation in transmission could be attributed to age.
Siik
S
S
Department of Ophthalmology, University of Oulu, Finland.
Airaksinen
P J
PJ
Tuulonen
A
A
Alanko
H I
HI
Nieminen
H
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging; D002648:Child; D004867:Equipment Design; D005260:Female; D005453:Fluorescence; D005470:Fluorometry / Q000295:instrumentation; D006801:Humans; D007908:Lens, Crystalline / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D011897:Random Allocation