Extending effects of phospholipids, cholesterol, and ethanolamines on survival of adult rat hepatocytes in serum-free primary culture.
In a serum-free primary culture, membrane lipids, such as phosphatidylethanolamine, phosphatidylcholine, or cholesterol, effectively prolonged the survival of adult rat hepatocytes. These lipids effectively prevented hepatocytes from morphologic degeneration observed in control cultures, such as enlargement of cell surface, degranulation of cytoplasm, and multinucleation. The maintenance effect of phospholipid precursors, ethanolamine, or phosphoethanolamine on the primary-cultured hepatocytes was similar to that of phospholipids. These effects appear to be due to stabilization of the plasma membrane.
Miyazaki
M
M
Department of Pathology, Okayama University Medical School, Japan.
Bai
L
L
Namba
M
M
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002784:Cholesterol / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology*; D046508:Culture Techniques / Q000379:methods*; D019856:Ethanolamine; D004983:Ethanolamines / Q000494:pharmacology*; D008099:Liver / Q000166:cytology*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000494:pharmacology*; D010714:Phosphatidylethanolamines / Q000494:pharmacology*; D051381:Rats
D003470:Culture Media; D004983:Ethanolamines; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D019856:Ethanolamine; C005448:phosphorylethanolamine; D002784:Cholesterol