Effect of the interferone inducer, polyriboinosinic.polyribocytidylic acid on rat liver regeneration following partial hepatectomy.
The administration of the interferone inducer, polyriboinosinic.polyribocytidylic acid, inhibited the rise of activities of thymidylate synthase and thymidine kinase as well as DNA content in 24 h-regenerating rat liver in a dose dependent manner. The immunoblotting assay showed that the decrease of thymidylate synthase activity was due to inhibition of the induction of the enzyme. Co-administration of putrescine did not affect the inhibitory effect of polyriboinosinic.polyribocytidylic acid. Polyriboinosinic acid did not affect DNA synthesis in rat liver regeneration.
Tsukamoto
I
I
Department of Food Science and Nutrition, Nara Women's University, Japan.
Kojo
S
S
D000818:Animals; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D006498:Hepatectomy; D015151:Immunoblotting; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D011070:Poly I-C / Q000494:pharmacology*; D011700:Putrescine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013937:Thymidine Kinase / Q000378:metabolism; D013940:Thymidylate Synthase / Q000096:biosynthesis
D013940:Thymidylate Synthase; D013937:Thymidine Kinase; D011070:Poly I-C; D011700:Putrescine