Malaria and Leishmania parasites share the knob-associated histidine-rich protein gene sequences.
1. The main coding region of the knob-associated histidine-rich protein (KAHRP) gene of Plasmodium falciparum hydridized with genomic DNA of Leishmania donovani. 2. A total of five EcoRI fragments of various sizes (7.5, 5.5, 3.2, 0.75 and 0.56 Kb) were recognized by this probe, under lower stringent conditions. However, under a higher stringency of washing, two of the smallest fragments were washed away. 3. Out of these EcoRI fragments, the 5.5 Kb band showed a maximum homology with the probe which contains the histidine-rich coding sequences, whereas the 3.2 Kb band showed none. Thus there is a possibility that the Leishmania parasite also contains a KAHRP-like gene.
Sharma
Y D
YD
Department of Biotechnology, All India Institute of Medical Sciences, New Delhi.
D000818:Animals; D016054:DNA, Protozoan / Q000235:genetics; D015246:Deoxyribonuclease EcoRI; D006639:Histidine / Q000235:genetics*; D007893:Leishmania donovani / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000235:genetics*; D010963:Plasmodium falciparum / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D016054:DNA, Protozoan; D010455:Peptides; D015800:Protozoan Proteins; C042968:knob protein, Plasmodium falciparum; D006639:Histidine; D015246:Deoxyribonuclease EcoRI