Modified Western blot assay for confirmation and differentiation of human T cell lymphotropic virus types I and II.
Disease association studies of human T cell lymphotropic virus (HTLV) types I and II are hindered by the need for multiple assays to confirm and differentiate between the viruses. A modified Western blot assay has been developed using HTLV-I viral lysate and unique (MTA-4) and shared (p21E) HTLV recombinant proteins. By defining confirmation of infection as the presence of antibodies to p24 gag protein and to p21E, all 56 HTLV-I and 49 HTLV-II antisera were confirmed by this modified Western blot alone. Differentiation was determined by reactivity to MTA-4. All HTLV-I antisera reacted with MTA-4 and all HTLV-II antisera did not react with MTA-4. These findings indicate the utility of selected HTLV-I recombinant proteins in a single assay format to confirm and differentiate infections with HTLV-I and HTLV-II.
Lipka
J J
JJ
Department of Pathology, Stanford University School of Medicine, Palo Alto, CA.
Santiago
P
P
Chan
L
L
Reyes
G R
GR
Samuel
K P
KP
Blattner
W A
WA
Shaw
G M
GM
Hanson
C V
CV
Sninsky
J J
JJ
Foung
S K
SK
D015153:Blotting, Western; D005189:False Positive Reactions; D015481:HTLV-I Antibodies / Q000097:blood; D015490:HTLV-I Infections / Q000175:diagnosis*; D015482:HTLV-II Antibodies / Q000097:blood; D015491:HTLV-II Infections / Q000175:diagnosis*; D015368:Human T-lymphotropic virus 1 / Q000276:immunology / Q000302:isolation & purification*; D015367:Human T-lymphotropic virus 2 / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D015531:Radioimmunoprecipitation Assay
D015481:HTLV-I Antibodies; D015482:HTLV-II Antibodies; D007106:Immune Sera