Proposal for a procedure for complete inactivation of the Creutzfeldt-Jakob disease agent.
We have examined complete inactivation conditions on brain homogenates from mice affected with Creutzfeldt-Jakob disease agent, and recommend for routine use a reliable procedure first treating the affected materials with 1 N NaOH for 60 min and then autoclaving at 121 degrees C for 30 min.
Taguchi
F
F
Department of Microbiology, School of Hygienic Sciences, Kitasato University, Kanagawa, Japan.
Tamai
Y
Y
Uchida
K
K
Kitajima
R
R
Kojima
H
H
Kawaguchi
T
T
Ohtani
Y
Y
Miura
S
S
D000818:Animals; D001921:Brain / Q000382:microbiology*; D007562:Creutzfeldt-Jakob Syndrome / Q000382:microbiology* / Q000635:transmission; D006358:Hot Temperature; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D012972:Sodium Hydroxide; D013242:Sterilization / Q000379:methods*; D013997:Time Factors
D012972:Sodium Hydroxide