Metabolism in the rat of a model xenobiotic plant metabolite S-benzyl-N-malonyl-L-cysteine.
1. The metabolism of a model xenobiotic plant metabolite S-benzyl-N-malonyl-L-cysteine (BMC) administered to rat at 10 mg/kg has been studied using a combination of radio-t.l.c. and h.p.l.c. 2. The major route of excretion for the administered 14C was via the urine (79% in 3 days). 3. The major metabolite was hippuric acid. The extent of biotransformation of BMC indicated the lability of the N-malonyl bond whose hydrolytic removal initiated a metabolic sequence which involved the action of C-S lyase to produce benzyl thiol. 4. A comparison of the findings from this study with those from experiments with N-acetyl-S-benzyl-L-cysteine and S-benzyl-L-cysteine is made to support the metabolic pathway proposed.
Richardson
K A
KA
Shell Research Ltd, Sittingbourne Research Centre, Kent, UK.
Edwards
V T
VT
Jones
B C
BC
Hutson
D H
DH
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003545:Cysteine / Q000031:analogs & derivatives* / Q000378:metabolism; D005966:Glucuronidase; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D010944:Plants / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D015262:Xenobiotics / Q000378:metabolism*
D015262:Xenobiotics; C070088:S-benzyl-N-malonylcysteine; D005966:Glucuronidase; D003545:Cysteine