In vitro studies on the radiosensitivity of multipotent hemopoietic progenitors in canine bone marrow.
The in vitro radiation response to 280-kV x-rays (does rate 72 cGy/min) of multipotent hemopoietic progenitor cells, mixed colony-forming units (CFU-mix), from canine bone marrow was assayed and compared to the radiation response characteristics of early erythroid progenitors, erythroid burst-forming units (BFU-E). To improve the colony-forming efficiency, the effect of various bone marrow cell separation techniques on colony formation of both progenitors was examined. The separation of bone marrow aspirates by discontinuous buoyant gradient centrifugation using the lymphocyte separation medium Lymphoprep with a density of 1.070 g/ml allowed the establishment of reproducible survival curves. The survival curves for both progenitors were strictly exponential, and CFU-mix were found to be more radiosensitive (D0 = 12 +/- 2 cGy) than BFU-E (D0 = 16 +/- 2 cGy).
Kreja
L
L
Institute for Occupational and Social Medicine, University of Ulm, Federal Republic of Germany.
Weinsheimer
W
W
Nothdurft
W
W
D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects*; D001854:Bone Marrow Cells; D002469:Cell Separation; D002470:Cell Survival / Q000528:radiation effects; D003114:Colony-Forming Units Assay; D004285:Dogs; D015672:Erythroid Precursor Cells / Q000528:radiation effects; D005260:Female; D006412:Hematopoietic Stem Cells / Q000528:radiation effects*; D066298:In Vitro Techniques; D008297:Male; D014965:X-Rays