Production of interleukin-1 beta-like factor with synovial cell growth promoting activity from adult T-cell leukemia cells.
We observed a case of adult T-cell leukemia (ATL) with proliferative synovitis. Culture supernatants from ATL cells (ATL-SN) obtained from the peripheral blood constitutively produced an interleukin-1 (IL-1)-like factor in vitro, as shown by the growth inhibition factor (GIF) assay using the A375 melanoma cell line and the lymphocyte activating factor (LAF) assay using C3H/HeJ thymocytes. Neutralization studies indicated that polyclonal antibodies against IL-1 beta blocked most (80%) of the activity in ATL-SN. In addition, increased amounts of IL-1 beta mRNA were found in the ATL cells by dot blot analysis. Sephacryl S-200 chromatography showed that the molecular weight of this factor was approximately 17.5 kDa, and Western blot analysis revealed that this factor reacted with polyclonal anti-IL-1 beta antibody under the reduced condition. The isoelectric point was 7.5. Furthermore, ATL-SN showed significant activity in promoting the growth of synovial cells in parallel with IL-1 activity. These data suggest that the constitutive production of this IL-1 beta-like factor might be responsible for proliferative synovitis in this case.
Miyasaka
N
N
Department of Virology and Immunology, Tokyo Medical and Dental University, Japan.
Higaki
M
M
Sato
K
K
Hashimoto
J
J
Taniguchi
A
A
Kohsaka
H
H
Yamamoto
K
K
Shichikawa
K
K
Nishioka
K
K
D000368:Aged; D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D007378:Interleukins / Q000032:analysis; D007526:Isoelectric Point; D015458:Leukemia, T-Cell / Q000150:complications / Q000378:metabolism / Q000473:pathology; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D008213:Lymphocyte Activation / Q000187:drug effects; D008546:Melanoma, Experimental / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D009363:Neoplasm Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D013583:Synovial Membrane / Q000187:drug effects*; D013585:Synovitis / Q000209:etiology; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000187:drug effects
D000906:Antibodies; D007375:Interleukin-1; D007378:Interleukins; D009363:Neoplasm Proteins