title: Suppression of murine leukemia virus-mediated 3Y1 cell fusion by expression of mouse MHC class I.
abstract: Rat 3Y1 fibroblasts transformed by adenovirus type 12 or its E1A gene formed syncytia by cocultivation with Friend murine leukemia virus (MuLV)-producing cells. On the other hand, parental 3Y1 cells and those derivatives induced by other tumor viruses or chemical carcinogen showed no MuLV-mediated syncytium formation [N. Momozaki et al. (1990) Arch. Virol. 115: 123-126]. The expression of major histocompatibility complex (MHC) class I mRNA and antigens was significantly reduced in these Ad12- and E1A-transformed 3Y1 cells. In contrast, other tumor virus-and chemical carcinogen-transformed 3Y1 cells expressed MHC class I almost in normal levels as did parental 3Y1 cells. Furthermore, Ad12-transformed 3Y1 cells which started to express the transfected exogenous MHC class I gene, H-2Ld, showed no more MuLV-mediated 3Y1 cell fusion. These results indicate that the expression of MHC class I on the cell membrane is closely related to the inhibition of 3Y1 cell fusion by MuLV.
authors.affiliation: Department of Biochemistry, Saga Medical School, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Momozaki
authors.forename: H
authors.initials: H
authors.lastname: Ogura
authors.forename: J
authors.initials: J
authors.lastname: Miyazaki
authors.forename: S
authors.initials: S
authors.lastname: Matsuhashi
authors.forename: K
authors.initials: K
authors.lastname: Joh
authors.forename: G
authors.initials: G
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Tabuchi
authors.forename: K
authors.initials: K
authors.lastname: Hori
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D002459:Cell Fusion; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D005622:Friend murine leukemia virus / Q000502:physiology*; D006183:H-2 Antigens / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D011401:Promoter Regions, Genetic; D014162:Transfection
chemical_list: D000199:Actins; D006183:H-2 Antigens