title: Purification and biochemical characterization of an invertebrate interleukin 1.
abstract: Interleukin 1 (IL-1) is a major immunoregulatory protein released by macrophages with many host defense related properties. That IL-1 has been found in the invertebrates attests to its importance in homeostasis. The first step in comparing the vertebrate protein to its invertebrate correlate is to purify the protein to study. We have purified to homogeneity IL-1 isolated from the coelomic fluid of the starfish Asterias forbesi. The IL-1 had isoelectric points of 7.4, 5.4 and 4.8. The pI 4.8 species had a molecular weight of 22,000 and the pI 7.4 and 5.4 species both had Mr of 17,000. Higher Mr forms were also found. These molecules were biologically active in the human melanoma A375 cytotoxicity assay for IL-1, and were also able to stimulate murine dermal fibroblast proliferation, protein synthesis, and PGE2 production. The pI 4.8 and 5.4 forms were purified to homogeneity and the amino acid composition was determined. The pI 4.8 and 5.4 species were purified more than 200-fold to specific activities of 3 x 10(6) and 1 x 10(6) units mg-1, respectively. The pI 7.4 form was isolated and partial N-terminal sequence analysis was performed. The similarities of molecular weight, isoelectric points and biological properties between vertebrate and invertebrate IL-1 show that it is an important, evolutionarily stable host defense molecule.
authors.affiliation: Department of Pathology, Health Sciences Center, State University of New York, Stony Brook 11794.
authors.forename: G
authors.initials: G
authors.lastname: Beck
authors.forename: G S
authors.initials: GS
authors.lastname: Habicht
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007375:Interleukin-1 / Q000737:chemistry / Q000302:isolation & purification*; D007526:Isoelectric Point; D008264:Macrophages / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D013215:Starfish / Q000276:immunology*
chemical_list: D007375:Interleukin-1