title: Detection of two abnormal hemoglobins, Hb Manitoba and Hb G-Coushatta, during analysis of glycohemoglobin (A1c) by high-performance liquid chromatography.
abstract: This report concerns the detection of two abnormal hemoglobins (Hb), Hb Manitoba and Hb G Coushatta, during analysis for glycohemoglobin (Hb A1c). Blood samples from two diabetic patients, analyzed for Hb A1c by HPLC, were found to contain additional Hb peaks. The presence of an abnormal Hb was confirmed in both instances by hemoglobinopathy studies. Structural studies determined the two Hb variants to be Hb Manitoba (alpha 2 102 Ser----Arg beta 2) and Hb G-Coushatta (alpha 2 beta 2 22 Glu----Ala). The significance of the presence of an abnormal Hb in Hb A1c analysis is discussed.
authors.affiliation: Department of Laboratory Medicine, Foothills Provincial General Hospital, University of Calgary, Alberta, Canada.
authors.forename: S C
authors.initials: SC
authors.lastname: Wong
authors.forename: M
authors.initials: M
authors.lastname: Tesanovic
authors.forename: M C
authors.initials: MC
authors.lastname: Poon
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003920:Diabetes Mellitus / Q000097:blood; D004588:Electrophoresis, Cellulose Acetate; D006442:Glycated Hemoglobin / Q000032:analysis*; D006455:Hemoglobins, Abnormal / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D014357:Trypsin
chemical_list: D006442:Glycated Hemoglobin; D006455:Hemoglobins, Abnormal; D014357:Trypsin; C033944:hemoglobin G Coushatta; C042249:hemoglobin Manitoba