Phagocytosis of opsonized yeast induces tumor necrosis factor-alpha mRNA accumulation and protein release by human polymorphonuclear leukocytes.
In this report we show that phagocytosis of yeast particles opsonized with IgG (Y-IgG) by human polymorphonuclear cells (PMN) results in the selective induction of tumor necrosis factor (TNF-alpha) messenger RNA (mRNA) and release of its mature protein. Lipopolysaccharide (LPS) was also found able to induce TNF-alpha secretion by PMN, but was a less potent stimulus compared with Y-IgG. There was no evidence of interleukin-6 (IL-6) gene expression in PMN after phagocytosis of Y-IgG or in response to LPS, whereas IL-6 mRNA expression and secretion were induced by either stimulus in monocytes. These findings demonstrate that a physiological function such as phagocytosis modulates the gene expression for a cytokine in PMN and shed new light on the understanding of the pathogenesis of the inflammatory process.
Bazzoni
F
F
Institute of General Pathology, University of Verona, Italy.
Cassatella
M A
MA
Laudanna
C
C
Rossi
F
F
D005656:Fungal Proteins / Q000378:metabolism*; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism*; D009895:Opsonin Proteins / Q000378:metabolism*; D010587:Phagocytosis; D012333:RNA, Messenger; D012441:Saccharomyces cerevisiae / Q000276:immunology* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism
D005656:Fungal Proteins; D015850:Interleukin-6; D009895:Opsonin Proteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha