title: The presence of N-glycosylated proteins in cell nuclei.
abstract: The protein-DNA crosslinking capability of cis-dichloro diammineplatinum has been exploited to check the intranuclear location of N-glycosylated proteins. When intact liver cells were treated with this reagent, a number of glycoproteins, recognized by Concanavalin A, have been shown to become crosslinked to DNA; many of them have been recognized as nuclear matrix components. The recognition by this lectin was abolished by treatment with N-glycosidase F, showing the presence of N-glycosidic bonds between the sugar moiety and the protein. Most of the glycoproteins appeared to have high mannose oligosaccharide chains, but sialic acid containing oligosaccharides were also identified.
authors.affiliation: Department of Biochemical Sciences A. Rossi Fanelli, University La Sapienza, Rome.
authors.forename: A
authors.initials: A
authors.lastname: Ferraro
authors.forename: P
authors.initials: P
authors.lastname: Grandi
authors.forename: M
authors.initials: M
authors.lastname: Eufemi
authors.forename: F
authors.initials: F
authors.lastname: Altieri
authors.forename: L
authors.initials: L
authors.lastname: Cervoni
authors.forename: C
authors.initials: C
authors.lastname: Turano
mesh_terms: D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000378:metabolism*; D002645:Chickens; D002945:Cisplatin / Q000494:pharmacology*; D003208:Concanavalin A; D003432:Cross-Linking Reagents; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D006031:Glycosylation; D008099:Liver / Q000378:metabolism*; D011485:Protein Binding; D013552:Swine
chemical_list: D003432:Cross-Linking Reagents; D006023:Glycoproteins; D003208:Concanavalin A; D004247:DNA; D002945:Cisplatin