Growth and cell kinetics of human hair papilla cells in vitro. An autoradiographic and flow cytometric study.
Hair papilla, a distinct specialized dermal compartment, plays a fundamental role in the biology of hair growth. Recently some attention has been focused on hair papilla cells (HPC) as possible targets for drugs influencing the hair growth. Isolation and cultivation of the HPC facilitates screening for such drugs. In the present work, growth and cell kinetics of human occipital scalp follicle HPC have been studied in vitro. HPC grow according to a Gompertz function, i.e. with considerable growth delay long before becoming confluent cultures, due probably to elongation of the potential doubling time (Tpot) and to a parallel increasing cell loss rate. The [3H]dT labelling index of the HPC strongly depends on the age of the subculture; the cycle time being about 4 days. A potential doubling time of about 93 h, indicative of growth fraction (GF) = 1, and a duration of S phase and G2 + M phase of about 8 h each were found by the combined application of continuous labelling with [3H]dT and DNA flow cytometry.
Bassukas
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Department of Dermatology, University of Erlangen-Nürnberg, Germany.
Arai
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A
Schell
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Hornstein
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OP
D000328:Adult; D001345:Autoradiography; D002455:Cell Division; D002478:Cells, Cultured; D004261:DNA Replication; D005434:Flow Cytometry; D006197:Hair / Q000166:cytology*; D006801:Humans; D007700:Kinetics; D008297:Male; D008433:Mathematics; D008940:Mitotic Index; D008954:Models, Biological; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium
D014316:Tritium; D013936:Thymidine