title: Choline acetyltransferase activity associated with cerebral cortical microvessels does not originate in basal forebrain neurons.
abstract: Cerebral cortical microvessels are innervated by cholinergic fibers that are probably involved in the regulation of local cerebral blood flow and blood-brain barrier permeability. The possibility exists that the cholinergic terminals associated with the cortical microvasculature belong to neurons from the nucleus basalis magnocellularis (NBM), where 70% of the cortical cholinergic projections originate. To test this hypothesis, ibotenic acid (25 nmol) was injected unilaterally in the NBM in rats, and 14 days later, choline acetyltransferase (ChAT) activity was measured in the frontoparietal cortex and in a blood vessel fraction isolated from this region. Lesions of the NBM resulted in a 50% decrease of cortical ChAT as compared with control or sham-operated hemispheres; however, no changes were observed in the ChAT activity associated with cortical microvessels. These results indicate that, in rat cerebral cortex, the perivascular cholinergic terminals do not originate in the basal forebrain.
authors.affiliation: Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Galea
authors.forename: C
authors.initials: C
authors.lastname: Fernández-Shaw
authors.forename: D
authors.initials: D
authors.lastname: Triguero
authors.forename: C
authors.initials: C
authors.lastname: Estrada
mesh_terms: D000818:Animals; D001479:Basal Ganglia / Q000201:enzymology*; D002196:Capillaries / Q000201:enzymology*; D002540:Cerebral Cortex / Q000098:blood supply* / Q000201:enzymology; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002795:Choline O-Acetyltransferase