title: Human serum stimulates endothelin-1 synthesis more potently than prostacyclin production by cultured vascular endothelial cells.
abstract: Human serum stimulated the synthesis of a vasoconstrictive peptide, endothelin-1 (ET-1), and a vasodilatory prostanoid, prostacyclin (PGI2), by cultured human umbilical vein endothelial cells in a concentration- and time-dependent manner. Incubation in 20% concentration of the serum for 24 h stimulated ET-1 synthesis almost six-fold while PGI2 production increased two-fold. In addition, a tumor-promoting phorbol ester, phorbol 12-myristate 13-acetate (PMA), inhibited the serum-induced ET-1 production and stimulated PGI2 synthesis in a concentration- and time-dependent manner. Our results suggest that human serum derived factor(s) stimulate the production of vasoconstrictive ET-1 more potently than the synthesis of vasodilatory PGI2 by human vascular endothelial cells and that the production of these agents is differentially regulated by PMA.
authors.affiliation: Children's Hospital, University of Helsinki, Finland.
authors.forename: A
authors.initials: A
authors.lastname: Ristimäki
authors.forename: R
authors.initials: R
authors.lastname: Renkonen
authors.forename: O
authors.initials: O
authors.lastname: Saijonmaa
authors.forename: O
authors.initials: O
authors.lastname: Ylikorkala
authors.forename: L
authors.initials: L
authors.lastname: Viinikka
mesh_terms: D001769:Blood; D002478:Cells, Cultured; D016232:Endothelins / Q000096:biosynthesis*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D011464:Epoprostenol / Q000096:biosynthesis*; D006801:Humans; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D016232:Endothelins; D011464:Epoprostenol; D013755:Tetradecanoylphorbol Acetate