Signal transduction mechanism of interleukin 6 in cultured rat mesangial cells.
Interleukin 6 (IL-6) is one of the potent autocrine growth factors for mesangial cells. We investigated the signal transduction mechanism of IL-6 in cultured rat mesangial cells. IL-6 induced a transient increase of inositol 1,4,5-trisphosphate (Ins 1,4,5-P3) followed by a transient and sustained increase of intracellular calcium concentration, suggesting that IL-6 stimulates phosphoinositide turnover. IL-6 also stimulated prostaglandin E2 (PGE2) production. The IL-6-concentration dependency in PGE2 production was similar to that in Ins 1,4,5-P3 production. We concluded that the action of IL-6 on mesangial cells is exerted at least partially through the enhancement of phosphoinositide turnover and PGE2 production.
Fukunaga
M
M
First Department of Medicine, Osaka University Medical School, Japan.
Fujiwara
Y
Y
Fujibayashi
M
M
Ochi
S
S
Yokoyama
K
K
Ando
A
A
Hirano
T
T
Ueda
N
N
Kamada
T
T
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism; D005920:Glomerular Mesangium / Q000378:metabolism*; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D015850:Interleukin-6 / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction / Q000502:physiology*; D010738:Type C Phospholipases / Q000378:metabolism
D015850:Interleukin-6; D011994:Recombinant Proteins; D015544:Inositol 1,4,5-Trisphosphate; D010738:Type C Phospholipases; D015232:Dinoprostone; D002118:Calcium