Expression of the GLUT1 glucose transporter increases thymidine uptake in Chinese hamster ovary cells at low glucose concentrations.
An increase in expression of the GLUT1 glucose transporter gene has been observed to be associated with an increase in glucose transport activity upon oncogenic transformation of the cells. Increased expression of this glucose transporter isoform has been also observed in fetal tissues. To investigate the consequences of this phenomenon on cellular metabolism and cell growth, an expression vector containing the GLUT1 glucose transporter complementary DNA was transfected into Chinese hamster ovary cells. Overexpression of this glucose transporter isoform resulted in an increase in not only glucose uptake and utilization but also thymidine uptake when cells were exposed to glucose-deficient conditions. This increase in glucose metabolism and DNA synthesis may play an important role on the growth and/or survival of cancer cells and fetal tissues.
Asano
T
T
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Shibasaki
Y
Y
Lin
J L
JL
Tsukuda
K
K
Katagiri
H
H
Ishihara
H
H
Yazaki
Y
Y
Oka
Y
Y
D019325:3-O-Methylglucose; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004261:DNA Replication / Q000187:drug effects; D003847:Deoxyglucose / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D007106:Immune Sera; D007700:Kinetics; D008757:Methylglucosides / Q000378:metabolism; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D009842:Oligopeptides / Q000138:chemical synthesis; D010053:Ovary; D013936:Thymidine / Q000378:metabolism*; D014162:Transfection
D007106:Immune Sera; D008757:Methylglucosides; D009004:Monosaccharide Transport Proteins; D009842:Oligopeptides; D019325:3-O-Methylglucose; D003847:Deoxyglucose; D005947:Glucose; D013936:Thymidine