title: Characterization of gingival epithelium epidermal growth factor receptor.
abstract: The binding characteristics of gingival epithelium epidermal growth factor (EGF) receptor were investigated using epithelial cell membranes from bovine gingiva. The binding of [125I]EGF was found to be time and protein concentration dependent, reversible, and specific. Unlabeled EGF competed for [125I]EGF binding with IC50 of 0.25nM and maximum displacement of 93% at 0.81nM. Scatchard analysis of the binding data inferred the presence of two binding sites, one of high affinity (Kd = 3.3 nM and Bmax = 47.3fmol/mg protein) and the other of a low affinity (Kd = 1.6 microM and Bmax = 1.9pmol/mg protein). Crosslinking of [125I]EGF to gingival membranes followed by polyacrylamide gel electrophoresis and autoradiography revealed a receptor protein of 170kDa.
authors.affiliation: Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
authors.forename: S
authors.initials: S
authors.lastname: Sengupta
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: S L
authors.initials: SL
authors.lastname: Wang
authors.forename: E
authors.initials: E
authors.lastname: Piotrowski
authors.forename: A
authors.initials: A
authors.lastname: Slomiany
authors.forename: B L
authors.initials: BL
authors.lastname: Slomiany
mesh_terms: D000818:Animals; D001345:Autoradiography; D001667:Binding, Competitive; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004815:Epidermal Growth Factor / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism*; D005881:Gingiva / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D011869:Radioligand Assay
chemical_list: D007328:Insulin; D004815:Epidermal Growth Factor; D066246:ErbB Receptors