Separation of proteins by consecutive sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and reversed phase high performance liquid chromatography.
A procedure for the preparative separation of proteins was developed by using consecutively sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (SDS-PAGE) and reversed phase high performance liquid chromatography (HPLC). The proteins were separated by SDS-PAGE and afterwards extracted from the gel. The extracted proteins were separated from SDS and other small molecular weight contaminants on a Fractogel TSK HW-40 (F) column in acidic aqueous acetonitrile. The proteins eluted from the Fractogel column were fractionated by HPLC. The identity and purity of the recovered proteins was confirmed by SDS-PAGE analysis.
Kaplan
B
B
Heller Institute of Medical Research, Chaim Sheba Medical Center, Tel-Hashomer, Israel.
Pras
M
M
D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007202:Indicators and Reagents; D007328:Insulin / Q000302:isolation & purification; D008970:Molecular Weight; D009113:Muramidase / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D012260:Ribonucleases / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate
D007202:Indicators and Reagents; D007328:Insulin; D011506:Proteins; D012967:Sodium Dodecyl Sulfate; D012260:Ribonucleases; D009113:Muramidase