title: Pregnancies after transfer of sheep embryos produced from oocytes matured and fertilized in vitro.
abstract: The experiments describe simple and effective methods used for the production of sheep embryos in vitro. The oocytes isolated from the ovarian follicles, 2-5 mm in diameter, were matured in culture for 22-24 h. After mixing with ram spermatozoa pretreated with heparin, 77-100% of fertilized oocytes were monospermic. Some of them (60-68.3%) cleaved in culture after 24-50 h to 2-4-cell stage. The transfer of cleaved embryos to the oviducts of 4 synchronized recipients resulted in pregnancies and 4 normal lambs were born at term.
authors.affiliation: Department of Genetics, Czechoslovak Academy of Sciences, Libĕchov.
authors.forename: T
authors.initials: T
authors.lastname: Slavík
authors.forename: J
authors.initials: J
authors.lastname: Fulka
mesh_terms: D000818:Animals; D002970:Cleavage Stage, Ovum; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006493:Heparin / Q000494:pharmacology; D008297:Male; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D011270:Pregnancy, Animal; D012756:Sheep / Q000196:embryology*; D013094:Spermatozoa / Q000502:physiology
chemical_list: D006493:Heparin