title: Method for the concentration of infectious pancreatic necrosis virus from hatchery water.
abstract: A method was developed for concentrating infectious pancreatic necrosis virus from hatchery water using positively charged 1-MDS filters. The method consists of passing large volumes (Ca. 1001) of hatchery water through 1-MDS microporous filter followed by the elution of the adsorbed virus using a high pH buffer. The virus adsorbed efficiently to 1-MDS filters when the pH of the water was 5.5 and was eluted optimally with 3% beef extract solution (pH 10). This procedure permitted the processing of 100 1 of hatchery water which resulted in a 300-fold reduction in the volume of water and greater than 90% recovery of the seeded virus.
authors.affiliation: Department of Veterinary Diagnostic Investigation, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
authors.forename: S
authors.initials: S
authors.lastname: Maheshkumar
authors.forename: S M
authors.initials: SM
authors.lastname: Goyal
authors.forename: R B
authors.initials: RB
authors.lastname: Peterson
authors.forename: P P
authors.initials: PP
authors.lastname: Economon
mesh_terms: D000327:Adsorption; D000535:Aluminum; D000077410:Aluminum Chloride; D017607:Aluminum Compounds; D000818:Animals; D002478:Cells, Cultured; D002712:Chlorides; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D012087:Reoviridae / Q000302:isolation & purification*; D014337:Trout / Q000382:microbiology*; D014871:Water Microbiology
chemical_list: D017607:Aluminum Compounds; D002712:Chlorides; D003470:Culture Media; D000077410:Aluminum Chloride; D000535:Aluminum