title: Antisuppressor mutations reduce misreading of the genetic code in Aspergillus nidulans.
abstract: Antisuppressor mutations were isolated in a strain containing the omnipotent suppressor suaC109. The antisuppressors reduce the activity of translational suppressors in vivo and counteract most aspects of the pleiotropic phenotype associated with the suaC and the suaA suppressor mutations. Using an homologous system for cell-free translation, we have measured translational accuracy in two antisuppressor strains with the genotype suaC109 and either the asuB11 or the asuD14 antisuppressor mutation. Ribosomes from antisuppressor mutants have higher levels of translation accuracy than those from the suppressor strain (suaC109, asu+). Mistranslation levels depended solely on the source of the sucrose-cleaned ribosomes. However, the increased accuracy associated with sucrose-cleaned ribosomes from antisuppressor strains can be nullified by salt-washing, suggesting that the component responsible can be washed off.
authors.affiliation: Biology Department, Birbeck College, University of London, UK.
authors.forename: S D
authors.initials: SD
authors.lastname: Martinelli
authors.forename: S
authors.initials: S
authors.lastname: Perera
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics*; D005815:Genetic Code; D011189:Potassium Chloride; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000378:metabolism; D013489:Suppression, Genetic
chemical_list: D011189:Potassium Chloride