title: Identification of the coat protein gene of bean golden mosaic geminivirus.
abstract: An open-reading frame designated 1R1, on DNA component 1 of bean golden mosaic geminivirus (BGMV), has been identified as the coat protein gene. A DraI restriction fragment of BGMV DNA1 that includes 1R1 was inserted into the SmaI pKK223-3 expression vector. The 32 kD protein expressed in Escherichia coli cells reacted with antibodies to the BGMV capsid polypeptide and behaved identically to purified capsid protein in western blots.
authors.affiliation: NODAI Research Institute, Tokyo University of Agriculture, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Murayama
authors.forename: Y
authors.initials: Y
authors.lastname: Yamaya
authors.forename: M
authors.initials: M
authors.lastname: Ikegami
mesh_terms: D000818:Animals; D002213:Capsid / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D015151:Immunoblotting; D009029:Mosaic Viruses / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D011817:Rabbits; D014162:Transfection
chemical_list: D004279:DNA, Viral