Interleukin-2 stimulation of blood mononuclear cells from patients with inflammatory bowel disease.
The functional capacity of biologically active, high-affinity interleukin-2 receptors (IL-2R) was studied by means of interleukin-2 (IL-2) stimulation of blood mononuclear cells (BMC) from 22 patients with inflammatory bowel disease (IBD) and 24 controls. The spontaneous, as well as the IL-2-induced, proliferative responses were significantly decreased in patients with active IBD, whereas the expressions of biologically inactive, low-affinity IL-2R (i.e. TAC antigen or CD25) were significantly increased in the same BMC cultures. In contrast, no significant differences were seen between patients and controls when BMC were stimulated with a nonspecific mitogen (phytohemagglutinin). The results suggest that a downregulation of IL-2 responsiveness may contribute to decreased BMC proliferation in vitro in active IBD.
Tvede
N
N
Laboratory of Medical Immunology TTA, Copenhagen University Hospital, Denmark.
Brynskov
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D015536:Down-Regulation / Q000502:physiology; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000097:blood* / Q000473:pathology; D007376:Interleukin-2 / Q000378:metabolism / Q000494:pharmacology* / Q000502:physiology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000494:pharmacology; D015375:Receptors, Interleukin-2 / Q000378:metabolism / Q000502:physiology
D007376:Interleukin-2; D010835:Phytohemagglutinins; D015375:Receptors, Interleukin-2