Detection of the illegal use of ethinylestradiol in cattle urine by gas chromatography-mass spectrometry.
A method for the detection of ethinylestradiol in cattle urine is described, based on enzymic hydrolysis of the sample, clean-up by means of disposable octadecyl and amino solid-phase extraction columns, fractionation by reversed-phase high-performance liquid chromatography, and detection by gas chromatography-mass spectrometry (selected-ion monitoring). Identification is based on both gas chromatographic and mass spectrometric data. The method has been tested on urine samples for a collaborative study and all the results found were correct.
Daeseleire
E
E
Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, State University of Ghent, Belgium.
De Guesquière
A
A
Van Peteghem
C
C
D000818:Animals; D002417:Cattle / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004997:Ethinyl Estradiol / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D015813:Substance Abuse Detection
D004997:Ethinyl Estradiol