Interleukin-1 is a mucus secretagogue.
Explant cultures of mouse duodenum were used to show that interleukin-1 (IL-1) causes release of mucus from epithelial goblet cells. Our experiments made use of a newly described enzyme-linked lectin assay (ELLA) which employs enzyme-conjugated soybean agglutinin to detect mucus glycoproteins secreted from explant cultures of mouse duodenum. Supernatants from cultures of lipopolysaccharide-stimulated peritoneal macrophages as well as partially purified rabbit alveolar macrophage-derived IL-1 and human rIL-1 beta all induced mucus release in a rapid and dose-dependent fashion. This observation may be important for investigating a link between the immune response and mucus hypersecretion from inflamed intestinal mucosa.
Cohan
V L
VL
Department of Immunology and Infectious Diseases, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205.
Scott
A L
AL
Dinarello
C A
CA
Prendergast
R A
RA
D000818:Animals; D015153:Blotting, Western; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009093:Mucus / Q000378:metabolism*; D009924:Organ Culture Techniques; D010529:Peritoneal Cavity / Q000166:cytology; D037121:Plant Lectins; D011453:Prostaglandins / Q000494:pharmacology; D011817:Rabbits; D030262:Soybean Proteins; D010835:Phytohemagglutinins
D007375:Interleukin-1; D037102:Lectins; D008070:Lipopolysaccharides; D037121:Plant Lectins; D011453:Prostaglandins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins