[Isolation and identification of N-terminal fragments of the A-alpha chain of bovine fibrinogen].
A method is suggested to obtain and purify N-end fragments of A alpha-chain from bovine fibrinogen. To identify the N-end chain fragments the fluorescent properties of tryptophan and tyrosine composing them as well as hydrolysis by ancistron H, a thrombin-like enzyme of the snake venom, have been used for the first time.
Pekhnik
I V
IV
Kheĭlomskiĭ
A B
AB
Chikurova
E V
EV
D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D005340:Fibrinogen / Q000737:chemistry / Q000302:isolation & purification*; D005344:Fibrinopeptide A / Q000378:metabolism; D005345:Fibrinopeptide B / Q000378:metabolism; D006868:Hydrolysis; D012697:Serine Endopeptidases / Q000737:chemistry; D012910:Snake Venoms; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014364:Tryptophan / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
D012910:Snake Venoms; D005344:Fibrinopeptide A; D005345:Fibrinopeptide B; D014443:Tyrosine; D014364:Tryptophan; D005340:Fibrinogen; D012697:Serine Endopeptidases; C063190:ancistron N
Vydelenie i identifikatsiia N-kontsevykh fragmentov A alpha-tsepi fibrinogena byka.