35S-beta-glucuronidase gene blocks biological effects of cotransferred iaa genes.
The iaaM and iaaH genes of Agrobacterium tumefaciens and Agrobacterium rhizogenes play an important role in crown gall and hairy root disease. The iaaM gene codes for tryptophan monooxygenase which converts tryptophan into indole-3-acetamide (IAM). IAM is converted into the auxin indole-3-acetic acid (IAA) by indoleacetamide hydrolase, encoded by the iaaH gene. In functional studies on the activity of the iaa genes of the TB region of the A. tumefaciens biotype III strain Tm4, the frequently used 35S-beta-glucuronidase (35S-UidA or GUS) marker gene was found to inhibit IAA synthesis and root induction encoded by the TB iaa genes. To exert this inhibition, the 35S-UidA gene must be cotransferred with the iaaH gene. The 35S promoter alone is sufficient to cause the inhibitory effect.
Tinland
B
B
Institut de Biologie Moléculaire des Plantes, Strasbourg, France.
Kares
C
C
Herrmann
A
A
Otten
L
L
D000581:Amidohydrolases / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics*; D007210:Indoleacetic Acids / Q000378:metabolism; D010944:Plants; D010947:Plants, Toxic; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012231:Rhizobium / Q000235:genetics; D014026:Nicotiana; D014170:Transformation, Genetic; D014365:Tryptophan Hydroxylase / Q000235:genetics* / Q000378:metabolism
D007210:Indoleacetic Acids; C030737:indoleacetic acid; C015950:indoleacetamide; D014365:Tryptophan Hydroxylase; D005966:Glucuronidase; D000581:Amidohydrolases; C029145:indoleacetamide hydrolase