Initial characterization of the carbohydrate structure of MCP-1.
MCP-1 is the principle monocyte chemoattractant produced by tumor cells in vitro. We previously identified different isoforms of MCP-1. Here we report that two predominant isoforms of MCP-1 (13 kD and 9 kD) can be distinguished by lectin binding. The results indicate that the disaccharide galactose-beta 1-3D-N-acetyl galactosamine is present on the 13 kD MCP-1 isoform but not the 9 kD isoform. Glycosylation of the 13 kD MCP-1 isoform does not appear to significantly change its antigenic properties and also does not affect its capacity to induce monocyte migration.
Jiang
Y
Y
Department of Oral Biology, Boston University Medical Center, MA 02118.
Tabak
L A
LA
Valente
A J
AJ
Graves
D T
DT
D002240:Carbohydrate Sequence; D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte; D004187:Disaccharides / Q000032:analysis*; D005910:Glioma; D006801:Humans; D015151:Immunoblotting; D037102:Lectins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009000:Monocytes / Q000187:drug effects / Q000502:physiology
D018932:Chemokine CCL2; D002630:Chemotactic Factors; D004187:Disaccharides; D037102:Lectins