PCR amplification of mRNA directly from a crude cell lysate prepared by thermophilic protease digestion.
Fung
M C
MC
Division of Biochemistry and Molecular Biology, John Curtin School of Medical Research, Australian National University, Canberra.
Fung
K Y
KY
D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004277:DNA, Single-Stranded / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D007376:Interleukin-2 / Q000235:genetics; D007377:Interleukin-3 / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*; D019914:Taq Polymerase; D013696:Temperature
D000199:Actins; D004277:DNA, Single-Stranded; D007376:Interleukin-2; D007377:Interleukin-3; D012333:RNA, Messenger; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase