title: Excimer laser ablation of fibrocartilage: an in vitro and in vivo study.
abstract: To date, lasers have found only limited applications in orthopedics. We employed a 308 nm XeCl excimer laser for ablation of fibrocartilage, in order to investigate the feasibility of excimer laser assisted meniscectomy. Experiments were conducted both in vitro and in vivo. For the in vitro study, human menisci, obtained during surgery and autopsy, were irradiated via a 600 microns core fiber at radiant exposures ranging between 20 mj/mm2 and 80 mj/mm2, at 20 Hz. Ablation rate measurements and histological analysis of the samples were performed. The ablation rates were found to range from 3 microns/pulse to 100 microns/pulse depending on the radiant exposure and/or the applied pressure on the fiber delivery system. Thermographic analysis was also performed during pulsed excimer as well as CW Nd:Yag and CW CO2 laser irradiation. Temperatures were lower for excimer laser (Tmax less than 65 degrees) than CW ND: Yag (Tmax less than 210 degrees) or CW CO2 (Tmax less than 202 degrees) laser. For the in vitro study, medial meniscectomy was performed in 15 rabbits with the excimer laser and a CW Nd:Yag laser in the right and left knee respectively. Excimer laser irradiation was performed at 70 mj/mm2. Nd:Yag irradiation was performed via a 600 microns core fiber at power outputs between 20 to 40 W for 10 and 20 seconds duration. The healing response to injury was investigated by histological analysis of the menisci after 1 day, 1, 2, 4, and 8 weeks following the laser procedure. Excimer laser treated menisci showed less inflammatory reaction and noticeable repair with minimal inflammatory response.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Orthopedic Surgery, University of Vienna, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Buchelt
authors.forename: T
authors.initials: T
authors.lastname: Papaioannou
authors.forename: M
authors.initials: M
authors.lastname: Fishbein
authors.forename: W
authors.initials: W
authors.lastname: Peters
authors.forename: C
authors.initials: C
authors.lastname: Beeder
authors.forename: W S
authors.initials: WS
authors.lastname: Grundfest
mesh_terms: D000818:Animals; D001182:Arthroscopy; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation; D053685:Laser Therapy / Q000379:methods*; D008592:Menisci, Tibial / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D009336:Necrosis; D011817:Rabbits; D014945:Wound Healing