Basal cell glycoprotein in pig epidermis closely resembles the beta 1 subunit of the integrin family of cell adhesion molecules.
A 135-kD conA-binding glycoprotein isolated from pig epidermis was previously localized to the surface of basal cells in stratified epithelia using affinity-purified antibodies. Preembedding immunoperoxidase electron microscopy has now shown that this glycoprotein is concentrated on the lateral surfaces of basal cells but is not detectable on those surfaces adjacent to the basement membrane indicating a role in cell-cell rather than cell-substrate interactions. The basal cell glycoprotein was shown to resemble the beta 1 subunit of the integrin family following the generation of a specific monoclonal antibody (M5.25). The epidermal glycoprotein recognized by M5.25 and by antibodies against the beta 1 fibronectin receptor from human placenta co-migrated on SDS gels under both reducing and non-reducing conditions. Its response to disulphide reducing agents was characteristic of beta 1 integrin subunits. In addition, the basal cell glycoprotein was shown to bind to the 120-kD cell-binding fragment of fibronectin in a RGD-dependent manner. It was readily detected by immunoblotting whole cell lysates of cultured pig keratinocytes suggesting increased expression in cultured cells compared to fresh epithelial tissue. The results suggest that beta 1 integrin subunits may be involved in cell-cell interactions between basal keratinocytes in pig epidermis and that these receptors are lost from the cell surface during terminal differentiation. Thus modulation of beta 1 integrin subunit expression may play an important role in regulating differentiation in pig epidermis.
King
I A
IA
Dermatology Research Group, MRC Clinical Research Centre, Middlesex, U.K.
Tabiowo
A
A
Fryer
P R
PR
Purkis
P E
PE
Leigh
I
I
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000737:chemistry*; D016023:Integrins / Q000032:analysis* / Q000737:chemistry; D012867:Skin / Q000737:chemistry / Q000166:cytology*; D013552:Swine
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D016023:Integrins