Measurement of brown adipose tissue uncoupling protein by enzyme linked immunosorbent assay.
Antibody to uncoupling protein (UCP) purified from rat brown adipose tissue (BAT) was raised in rabbits and an enzyme linked immunosorbent assay was developed. The antiserum did not cross-react with other mitochondrial proteins from BAT and from other tissues but cross-reacted with UCP from hamster, guinea pig and mouse. The assay is capable of detecting 5 ng of UCP. Using this assay and a crude mitochondrial preparation, UCP content of BAT was shown to increase during cold adaptation.
Chan
E L
EL
Department of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, N.T.
Swaminathan
R
R
D000222:Adaptation, Physiological; D002001:Adipose Tissue, Brown / Q000737:chemistry*; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins; D003080:Cold Temperature; D006224:Cricetinae; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D007473:Ion Channels; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D051379:Mice; D008928:Mitochondria / Q000737:chemistry*; D008930:Mitochondria, Liver / Q000737:chemistry; D008931:Mitochondria, Muscle / Q000737:chemistry; D024101:Mitochondrial Proteins; D051381:Rats; D011919:Rats, Inbred Strains; D014475:Uncoupling Agents / Q000032:analysis* / Q000276:immunology; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D014475:Uncoupling Agents; D000071256:Uncoupling Protein 1