title: Changes in the kinetics of inositol transport during TPA-induced differentiation of HL60 cells towards monocytes.
abstract: When exposed to the phorbol ester TPA, HL60 cells undergo growth arrest and differentiate towards monocytes. During TPA-induced differentiation there was a 2.6-fold increase in the rate of inositol transport (Vmax), a 2.1-fold increase in intracellular inositol and a 1.5-fold increase in inositol lipid. An increase in the Vmax of inositol transport did not occur when the variant cell line HL60Ast3 was exposed to TPA, which has been shown in this cell line to induce growth arrest but not differentiation. This observation suggests that the change in inositol transport during HL60 monocyte differentiation is specifically associated with the process of cell differentiation as opposed to growth arrest.
authors.affiliation: Department of Medicine, University of Birmingham, Edgbaston, UK.
authors.forename: G
authors.initials: G
authors.lastname: Grafton
authors.forename: C M
authors.initials: CM
authors.lastname: Bunce
authors.forename: M C
authors.initials: MC
authors.lastname: Sheppard
authors.forename: G
authors.initials: G
authors.lastname: Brown
authors.forename: M A
authors.initials: MA
authors.lastname: Baxter
mesh_terms: D001692:Biological Transport; D002454:Cell Differentiation / Q000187:drug effects; D006801:Humans; D007294:Inositol / Q000378:metabolism*; D007700:Kinetics; D007938:Leukemia; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D010587:Phagocytosis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D007294:Inositol; D013755:Tetradecanoylphorbol Acetate