Secretion of interleukin-1 by bovine milk macrophages.
The relative sensitivity of bovine blood monocytes and macrophages isolated from milk to lipopolysaccharide, with respect to interleukin 1 (IL-1) production, was evaluated. Addition of lipopolysaccharide (0 to 30 microgram/ml) to the culture medium resulted in increases in secreted and intracellular IL-1 activity for monocytes and milk macrophages, with maximal stimulation achieved at 30 micrograms of lipopolysaccharide/ml of medium. At this concentration of lipopolysaccharide, monocytes released 76% of the total IL-1, whereas milk macrophages released only 26% of the total IL-1 produced within the cell. Secretion of a small quantity of IL-1 was a common property of macrophages isolated from healthy and mastitic quarters. We concluded that limited secretion of IL-1 may render the milk macrophages less efficient in promoting lymphocyte activation.
Politis
I
I
Department of Animal Science, McGill University, Ste Anne de Bellevue, Quebec.
McBride
B W
BW
Burton
J H
JH
Zhao
X
X
Turner
J D
JD
D000818:Animals; D002417:Cattle / Q000276:immunology*; D002478:Cells, Cultured; D005260:Female; D007111:Immunity, Cellular; D007375:Interleukin-1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000276:immunology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D008414:Mastitis, Bovine / Q000276:immunology*; D008892:Milk / Q000166:cytology* / Q000276:immunology; D009000:Monocytes / Q000276:immunology
D007375:Interleukin-1; D008070:Lipopolysaccharides