Determination of bencyclane in human plasma by means of capillary gas chromatography and nitrogen-phosphorus selective detection.
A sensitive and specific method for the determination of bencyclane in human plasma is presented. Bencyclane was extracted from human plasma with two 3-ml volumes of isooctane and was shaken for 10 min. The organic phase was separated and evaporated to dryness at 40 degrees C under a nitrogen stream. The residue was dissolved and an aliquot was injected into the gas chromatograph. The separation was performed with a DB-17 column with helium as the carrier gas. Nitrogen-selective detection was performed. The quantification was performed with the signal output. The limit of detection was 1 ng/ml.
Lohmann
A
A
R & D, Referat Pharmakokinetik, Thiemann Arzneimittel GmbH, Waltrop, Germany.
Diekmann
N
N
Walters
R
R
Kraft
R
R
D001537:Bencyclane / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D009584:Nitrogen; D009795:Octanes; D010758:Phosphorus
D009795:Octanes; D010758:Phosphorus; D001537:Bencyclane; D009584:Nitrogen; C045798:2,2,4-trimethylpentane