The effect of some immunomodulators administration to rats on palmitic and oleic acids incorporation into the lipids of liver cell organelles.
The incorporation of equimolar doses of [14C]-palmitic and [3H]-oleic acids into the lipid moiety of hepatocytes after muramyl dipeptide (MDP), adamantylamide dipeptide (AdDP) and levamisole (LM) administration were investigated. The utilization of [14C]-palmitic acid for the synthesis of neutral lipids and phospholipids of crude nuclear fraction increased significantly after MDP and AdDP administration and to a lesser extent after LM. While the incorporation of [3H]-oleic acid did not change significantly after MDP and AdDP, LM significantly increased the incorporation of this acid into the phospholipids of nuclear and microsomal fractions. The changes in the incorporation of saturated palmitic and unsaturated oleic acids suggest a possible influence on deacylation-reacylation mechanisms. These changes could alter the physical properties of membrane and affect certain membrane functions, including the activity of membrane bound enzymes and transport mechanisms.
Buchar
E
E
Institute of Pharmacology, Czechoslovak Academy of Sciences, Prague.
Farghali
H
H
Janků
I
I
Masek
K
K
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology*; D000547:Amantadine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D004151:Dipeptides / Q000494:pharmacology*; D007978:Levamisole / Q000494:pharmacology*; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism* / Q000493:pharmacokinetics; D015388:Organelles / Q000378:metabolism*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism* / Q000493:pharmacokinetics; D010743:Phospholipids / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000378:metabolism; D014316:Tritium
D004151:Dipeptides; D008055:Lipids; D009829:Oleic Acids; D010169:Palmitic Acids; D010743:Phospholipids; D014316:Tritium; D007978:Levamisole; D019301:Oleic Acid; D019308:Palmitic Acid; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C044891:adamantylamide-alanyl-isoglutamine; D000547:Amantadine