[Increase in the quantity of highly repetitive sequences in extrachromosomal circular DNAs under inhibition of translation by cycloheximide].
Small polydispersed circular DNA(spcDNA) was isolated from cultivated HeLa cells. Cells were treated by cycloheximide in concentrations of 1 and 50 micrograms/ml. Gradient fractions were dot blotted to nitrocellulose filters and were hybridized with different repetitive DNAs. The pool of repetitive DNA sequences in fraction of spcDNA increased for cycloheximide treated cells. The content of Alu sequences increased by 1.5-2.5 times, "classical" satellite DNA--by 5-7 times, alpha-satellite--by 3-5 times.
Krokhina
T B
TB
Raevskaia
G B
GB
Chernyĭ
D I
DI
Gar'kavtsev
I V
IV
D003513:Cycloheximide / Q000494:pharmacology*; D004247:DNA; D004270:DNA, Circular / Q000187:drug effects / Q000302:isolation & purification; D004276:DNA, Satellite; D005111:Extrachromosomal Inheritance; D006367:HeLa Cells; D006801:Humans; D014176:Protein Biosynthesis / Q000187:drug effects; D012091:Repetitive Sequences, Nucleic Acid
D004270:DNA, Circular; D004276:DNA, Satellite; D004247:DNA; D003513:Cycloheximide
Uvelichenie soderzhaniia vysokopovtoriaiushchikhsia posledovatel'nosteĭ v ékstrakhromosomnykh kol'tsevykh DNK pri tormozhenii transliatsii tsiklogeksimidom.