[The characteristics of the hematopoietic cells forming colonies on cellulose acetate membranes and their distinctions from other clonogenic cells].
In order to characterize hemopoietic cells forming colonies on membranes of cellulose acetate (CFU-acm) implanted into the peritoneal cavity of mice, we studied the effect of factors stimulating and inhibiting granulocytopoiesis on these cells. Proliferation of CFU-acm can be controlled by humoral factors and this allows us to conclude that they are not identical to CFUs and probably belong to the compartment of early hemopoietic cells of the granulocyte series. We also present evidence for fractional composition, ultrastructure and bone marrow origin of cells belonging to the layer providing for hemopoietic microenvironment for the resulting foci of hemopoiesis; we also present evidence for the role of fibroblasts (fibroblast-like cells) in the maintenance of hemopoiesis. Experiments on transplantation of bone marrow from several rodent species to syn-, allo- and xenogenic recipients allowed us to study interactions of hemopoietic elements of colonies with cells of the underlying layer.
Michurina
T V
TV
Satdykova
G P
GP
Balazs
A
A
Butorina
N N
NN
Mushegova
N I
NI
Khrushchov
N G
NG
D000818:Animals; D016026:Bone Marrow Transplantation; D002482:Cellulose / Q000031:analogs & derivatives; D002999:Clone Cells / Q000187:drug effects / Q000648:ultrastructure; D003114:Colony-Forming Units Assay; D006224:Cricetinae; D005260:Female; D006168:Guinea Pigs; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000648:ultrastructure*; D008567:Membranes, Artificial; D008647:Mesocricetus; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008851:Micropore Filters; D019736:Prostheses and Implants; D051381:Rats
D008567:Membranes, Artificial; C005062:acetylcellulose; D002482:Cellulose
Kharakteristika krovetvornykh kletok, obrazuiushchikh kolonoiĭ na atsetat-tselliuloznykh membranakh, ikh otlichiia ot drugkikh klonogennykh kletok.