Inhibitory effects of ovomacroglobulin on bacterial keratitis in rabbits.
We studied the inhibitory effects of chicken egg-white ovomacroglobulin (ovoM) on keratitis induced by 56,000-Da protease (56 KP) of Serratia marcescens and by elastase (PE) and alkaline protease (PAP) of Pseudomonas aeruginosa. The effects of ovoM on the serratial and pseudomonal keratitis in rabbits were also elucidated. In one model, four drops of 56 KP, PE, or PAP (1 mg/ml) were applied to wounded corneas of eight eyes. Thereafter, 80 microliters ovoM (10 mg/ml) was dropped into four eyes and 0.01 M phosphate-buffed 0.15 M saline (pH 7.4) into the other eyes as a control. The other in vivo test system involved intrastromal injection of S. marcescens or P. aeruginosa, by which each sample (10(5)-10(7) colony-forming units) mixed with ovoM was injected into one cornea and the other cornea received organisms without ovoM. OvoM completely inhibited the activity of these bacterial proteases in vitro and reduced corneal destruction in experimental keratitis in rabbits. In addition, greatly accelerated wound healing was observed.
Miyagawa
S
S
Department of Ophthalmology, Kumamoto University Medical School, Japan.
Kamata
R
R
Matsumoto
K
K
Okamura
R
R
Maeda
H
H
D000287:Administration, Topical; D000818:Animals; D001426:Bacterial Proteins; D015169:Colony Count, Microbial; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000473:pathology; D015818:Eye Infections, Bacterial / Q000188:drug therapy* / Q000473:pathology; D007634:Keratitis / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D008259:Macroglobulins / Q000627:therapeutic use*; D008297:Male; D008666:Metalloendopeptidases; D000073718:Pancreatitis-Associated Proteins; D011480:Protease Inhibitors / Q000627:therapeutic use*; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000473:pathology; D011817:Rabbits; D012697:Serine Endopeptidases; D012706:Serratia marcescens / Q000187:drug effects; D000511:alpha-Macroglobulins
D001426:Bacterial Proteins; D008259:Macroglobulins; D000073718:Pancreatitis-Associated Proteins; D011480:Protease Inhibitors; C000612141:REG3A protein, human; D000511:alpha-Macroglobulins; C036828:ovomacroglobulin; C038495:Pseudomonas serine proteinase; D012697:Serine Endopeptidases; D008666:Metalloendopeptidases; C044194:microbial metalloproteinases; C043859:pseudolysin, Pseudomonas aeruginosa