[Production of a fibrinogen concentrate from small amounts of human autologous blood plasma and its short-term characteristics].
Simple procedure was developed for production of fibrinogen concentrate from small amounts of human autogenous blood using precipitation with polyethylene glycol and ammonium sulfate. Maximal yield of fibrinogen was obtained using polyethylene glycol 2,000 and 6,000 daltons at concentrations 7% and 4.5%, respectively. The fibrinogen preparations included fibronectin, inhibitors of proteinases, factor XIII of blood coagulation, plasminogen. Sigma-Aminocapronic acid and contrical should be added during bloodletting as well as into blood plasma before isolation of fibrinogen to avoid possible fibrinogen proteolysis.
Veremeenko
K N
KN
Varetskaia
T V
TV
Kizim
A I
AI
Svital'skaia
L A
LA
D001769:Blood; D004591:Electrophoresis, Polyacrylamide Gel; D005176:Factor XIII / Q000737:chemistry; D005340:Fibrinogen / Q000032:analysis / Q000302:isolation & purification*; D005353:Fibronectins / Q000032:analysis; D006801:Humans; D010958:Plasminogen / Q000032:analysis; D011092:Polyethylene Glycols / Q000737:chemistry; D011480:Protease Inhibitors / Q000032:analysis
D005353:Fibronectins; D011480:Protease Inhibitors; D011092:Polyethylene Glycols; D005340:Fibrinogen; D010958:Plasminogen; D005176:Factor XIII
Poluchenie kontsentrata fibrinogena iz nebol'shikh kolichestv plazmy autogennoĭ krovi cheloveka i ego kratkaia kharakteristika.