[The biochemical and immunological properties of group A type M 29 streptococci cultured in the presence of a bovine blood serum preparation].
The influence of the preparation of cattle blood serum on group A streptococcus, type M 29, has been studied. The study has revealed that the addition of 17% of dialysis water obtained from a fraction of cattle blood serum to the standard culture medium (3% Todd-Hewitt broth) produces changes in the amino acid composition of the cell walls of M+ variant without altering the antiphagocytic resistance of the mutant thus obtained. The dialysate of the pepsin digest of the cell walls of the mutant contains Fc-receptors and receptors to fibrinogen, while the initial strain contains only receptors to fibrinogen which are, in this case, the pepsin fragments of M protein. The study has revealed similarity in the amino acid compositions of these proteins (receptors to fibrinogens) of phenotypes M+ and M2+. Thus, our data confirm that the initial strain and the mutant belong to different phenotypes of group A streptococcus, type M 29.
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D000596:Amino Acids / Q000032:analysis / Q000187:drug effects; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis / Q000187:drug effects; D001798:Blood Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D002417:Cattle; D002473:Cell Wall / Q000737:chemistry / Q000187:drug effects; D003470:Culture Media; D008970:Molecular Weight; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology; D010641:Phenotype; D012692:Serial Passage; D013297:Streptococcus pyogenes / Q000032:analysis / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
D000596:Amino Acids; D001426:Bacterial Proteins; D001798:Blood Proteins; D003470:Culture Media
Biokhimicheskie i immunologicheskie svoĭstva streptokokkov gruppy A tipa M 29, kul'tivirovannykh v prisutstvii preparata iz syvorotki krovi krupnogo rogatogo skota.