Binding specificity of human nuclear protein interacting with the Alu-family DNA repeats.
We have demonstrated earlier that human cells contain nuclear protein interacting with conserved GC-rich sequence motifs of human Alu-family DNA repeats. One of these sequences is located in the region between elements A and B of bipartite RNA polymerase III promoter of Alu (AB-region). In this study we have used a DNase I footprinting assay with an Alu restriction subfragment covering AB-region, as well as a gel mobility shift assay with appropriate synthetic oligonucleotides to analyse in more detail the interaction of the protein with AB-region. We have also used antibodies raised against a zinc-finger peptide to examine the presence of a zinc-finger in the Alu-binding protein. The results indicate that AGG triplets may be important for high-affinity binding of the protein to DNA, and that the Alu-binding protein is a zinc-finger protein.
Chesnokov
I
I
Institute of Cytology USSR Academy of Sciences, Leningrad.
Bozhkov
V
V
Popov
B
B
Tomilin
N
N
D000595:Amino Acid Sequence; D001483:Base Sequence; D001667:Binding, Competitive; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D012320:RNA Polymerase III / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D016335:Zinc Fingers / Q000235:genetics
D009687:Nuclear Proteins; D015345:Oligonucleotide Probes; D004247:DNA; D012320:RNA Polymerase III