title: Effect of acetylpolyamines on in vitro protein synthesis and on the growth of a polyamine-requiring mutant of Escherichia coli.
abstract: The functions of acetylpolyamines were examined with respect to stimulation of protein synthesis and cell growth. Unlike polyamines, acetylpolyamines could not lower the optimal Mg2+ concentration of protein synthesis, and the degree of stimulation of protein synthesis by acetylpolyamines was small. The addition of N1-acetylspermine did not stimulate cell growth of a polyamine-requiring mutant of Escherichia coli MA261, although acetylspermine was accumulated in the cells. Acetylspermine did not interfere with polyamine stimulation of protein synthesis and cell growth of E. coli MA261. The binding of acetylpolyamines to RNA was very weak, and the binding of polyamines to RNA was not disturbed significantly by the presence of acetylpolyamines. When the growth of E. coli MA261 was stimulated by addition of polyamines, significant amounts of acetylpolyamines were also formed in the cells. These results suggest that acetylation of polyamines, together with polyamine excretion, may regulate the intracellular level of the parent polyamines when excess amounts of polyamines accumulate intracellularly.
authors.affiliation: Faculty of Pharmaceutical Sciences, Chiba University.
authors.forename: T
authors.initials: T
authors.lastname: Kakegawa
authors.forename: Y
authors.initials: Y
authors.lastname: Guo
authors.forename: Y
authors.initials: Y
authors.lastname: Chiba
authors.forename: T
authors.initials: T
authors.lastname: Miyazaki
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: S
authors.initials: S
authors.lastname: Hirose
authors.forename: Z N
authors.initials: ZN
authors.lastname: Canellakis
authors.forename: K
authors.initials: K
authors.lastname: Igarashi
mesh_terms: D000107:Acetylation; D002474:Cell-Free System; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005914:Globins / Q000096:biosynthesis* / Q000235:genetics; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D010452:Peptide Biosynthesis; D010455:Peptides; D011073:Polyamines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012358:RNA, Transfer, Met; D012270:Ribosomes / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D010455:Peptides; D011073:Polyamines; D012333:RNA, Messenger; D012346:RNA, Transfer, Amino Acyl; D012358:RNA, Transfer, Met; C024988:tRNA, formylmethionine-; C012001:polyphenylalanine; D005914:Globins; D008274:Magnesium