title: Environmental conditions differentially affect vir gene induction in different Agrobacterium strains. Role of the VirA sensor protein.
abstract: The induction of vir gene expression in different types of Agrobacterium strains shows different pH sensitivity profiles. The pH sensitivity pattern demonstrated by octopine Ti strains was similar to that of a supervirulent leucinopine Ti strain, whereas this was different from that shown by nopaline Ti strains and agropine Ri strains. Data are given which indicate that these differences are due to different properties of the virA genes of these wild types. An exceptional case was formed by strains with the limited-host-range plasmid pTiAG57 which showed AS-dependent vir induction only if reduced inoculum sizes were used and the temperature was 28 degrees C or below.
authors.affiliation: Clusius Laboratory, Leiden University, Netherlands.
authors.forename: S C
authors.initials: SC
authors.lastname: Turk
authors.forename: L S
authors.initials: LS
authors.lastname: Melchers
authors.forename: H
authors.initials: H
authors.lastname: den Dulk-Ras
authors.forename: A J
authors.initials: AJ
authors.lastname: Regensburg-Tuïnk
authors.forename: P J
authors.initials: PJ
authors.lastname: Hooykaas
mesh_terms: D000098:Acetophenones / Q000494:pharmacology; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D010957:Plasmids / Q000235:genetics; D012231:Rhizobium / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D013696:Temperature; D015533:Transcriptional Activation; D037521:Virulence Factors
chemical_list: D000098:Acetophenones; D001426:Bacterial Proteins; D037521:Virulence Factors; C051667:acetosyringone; D001120:Arginine