Rapid identification of specific genes in E. coli by hybridization to membranes containing the ordered set of phage clones.
A simplified protocol for the hybridization of labeled oligonucleotides and other radiolabeled DNAs to membranes is described and used for the detection of specific recombinant phage in the ordered miniset collection representing virtually the entire E. coli genome.
Noda
A
A
Biotechnology Research Labs, Takara Shuzo Co., Ltd., Shiga, Japan.
Courtright
J B
JB
Denor
P F
PF
Webb
G
G
Kohara
Y
Y
Ishihama
A
A
D001483:Base Sequence; D003090:Coliphages / Q000235:genetics; D004274:DNA, Recombinant / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization
D004274:DNA, Recombinant; D004279:DNA, Viral; D008567:Membranes, Artificial