[Isolation of antigen-active biopolymers from Candida maltosa culture fluid for obtaining allergens of diagnostic value for examination of industrial microbiology workers].
The membrane filtration technique and column gel chromatography+ (with sepharose 6B or sephacryl S-200) were used to receive fractions of KJ Candida maltosa, strain VSB 899, which were studied with highly effective liquid chromatography, immunochemical reactions and intracutaneous allergotesting on guinea-pigs sensibilized with VSB 899 live cultures. In the whole KJ strain VSB 899 concentrate, 8 components were identified, six of which had molecular masses from 350,000 to 18,000 and had no analogue in the surface antigen preparation (SAP) from the same strain of yeast-like fungi. It was shown that the SAP-related antigen determinants were present in predominantly polysaccharide-containing fractions. In intracutaneous allergotesting, chromatographic fractions with molecular masses of 50,000 and 18,000-35,000 were most manifesting. It was supposed that still further purification of the allergen will increase its specificity.
Kravtsov
E G
EG
Gukasian
I A
IA
Dolgikh
M S
MS
Ermolaev
A V
AV
Spivak
S M
SM
D000485:Allergens / Q000302:isolation & purification*; D000818:Animals; D000946:Antigens, Fungal / Q000302:isolation & purification*; D002175:Candida / Q000276:immunology*; D002177:Candidiasis / Q000175:diagnosis* / Q000276:immunology; D002850:Chromatography, Gel / Q000379:methods; D003470:Culture Media; D006168:Guinea Pigs; D006801:Humans; D007218:Industrial Microbiology / Q000379:methods*; D009784:Occupational Diseases / Q000175:diagnosis* / Q000276:immunology; D009787:Occupational Medicine / Q000379:methods*
D000485:Allergens; D000946:Antigens, Fungal; D003470:Culture Media
Vydelenie antigenoaktivnykh biopolimerov kul'tural'noĭ zhidkosti Candida maltosa s tsel'iu polucheniia diagnosticheski tsennykh aller- genov dlia obsledovaniia rabochikh mikrobiologicheskikh proizvodstv.