Erythroid progenitors in tumor-bearing mice: erythroid inhibitory factors produced by adenocarcinoma-755.
Adenocarcinoma-755-bearing C57BL/6 mice developed anemia with the growth of the tumors. The numbers of granuloid and monocytoid progenitors (colony-forming unit in culture, CFU-C) of the bone marrow and spleen and the peripheral blood WBC counts increased in tumor-bearing mice. The erythroid progenitors (erythroid colony-forming units, CFU-E; erythroid burst-forming units, BFU-E) of the bone marrow showed a marked decrease, overcoming their increase in the spleen in tumor-bearing mice. Fractionation of conditioned medium of tumor cells led to the isolation of a protein of 80-kd molecular weight that stimulated murine CFU-C growth and inhibited the CFU-E and BFU-E growth in a dose-dependent manner. These results indicate that erythroid inhibitory factors produced by tumors exist in tumor-bearing mice. Erythroid inhibitory factors might also exist in non-hematological malignancies of humans, and they might be one of the mechanisms of anemia.
Aoki
I
I
Second Department of Internal Medicine, Kyorin University School of Medicine, Tokyo, Japan.
Toyama
K
K
Abe
N
N
Yamamoto
M
M
Ishikawa
K
K
D000230:Adenocarcinoma / Q000097:blood / Q000473:pathology* / Q000601:surgery; D000818:Animals; D001772:Blood Cell Count; D001853:Bone Marrow / Q000473:pathology*; D003114:Colony-Forming Units Assay; D003470:Culture Media; D015672:Erythroid Precursor Cells / Q000473:pathology*; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000097:blood / Q000473:pathology* / Q000601:surgery; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D013154:Spleen / Q000166:cytology; D013156:Splenectomy; D013997:Time Factors; D014407:Tumor Cells, Cultured
D003470:Culture Media