title: Identification of profilin as a novel pollen allergen; IgE autoreactivity in sensitized individuals.
abstract: A complementary DNA encoding a pollen allergen from white birch (Betula verrucosa) that was isolated from a pollen complementary DNA library with serum immunoglobulin E from a birch pollen-allergic individual revealed significant sequence homology to profilins. The recombinant protein showed high affinity to poly-L-proline. Immunoglobulin E antibodies from allergic individuals bound to natural and recombinant birch profilin and also to human profilin. In addition, birch and human profilin induced histamine release from blood basophils of profilin-allergic individuals, but not of individuals sensitized to other plant allergens. The structural similarity of conserved proteins might therefore be responsible for maintaining immunoglobulin E antibody titers in type I allergy.
authors.affiliation: Institute of General and Experimental Pathology, University of Vienna, Austria.
authors.forename: R
authors.initials: R
authors.lastname: Valenta
authors.forename: M
authors.initials: M
authors.lastname: Duchêne
authors.forename: K
authors.initials: K
authors.lastname: Pettenburger
authors.forename: C
authors.initials: C
authors.lastname: Sillaber
authors.forename: P
authors.initials: P
authors.lastname: Valent
authors.forename: P
authors.initials: P
authors.lastname: Bettelheim
authors.forename: M
authors.initials: M
authors.lastname: Breitenbach
authors.forename: H
authors.initials: H
authors.lastname: Rumpold
authors.forename: D
authors.initials: D
authors.lastname: Kraft
authors.forename: O
authors.initials: O
authors.lastname: Scheiner
mesh_terms: D000595:Amino Acid Sequence; D003285:Contractile Proteins / Q000276:immunology; D015723:Gene Library; D006801:Humans; D006967:Hypersensitivity; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology*; D008840:Microfilament Proteins / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D011058:Pollen / Q000276:immunology*; D051304:Profilins; D011994:Recombinant Proteins / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D003285:Contractile Proteins; D008840:Microfilament Proteins; D051304:Profilins; D011994:Recombinant Proteins; D007073:Immunoglobulin E