title: Autoradiographic localization of binding sites for second messengers on neurones and astrocytes of cultured rat cerebellum.
abstract: Using autoradiographic techniques, studies on the cellular localization of binding sites for second messengers in explant cultures of rat cerebellum have been performed. Binding of [3H]inositoltrisphosphate ([3H]IP3), [3H]phorbol dibutyrate ([3H]PDBu) occurred to Purkinje cells whereas binding sites for [3H]forskolin were mainly confined to granule cells and outgrowing nerve fibres. A great number of astrocytes was also labelled by all three radio-ligands. Binding of [3H]IP3, [3H]PDBu and [3H]forskolin was displaced by unlabelled compounds, suggesting specific binding. Our observations provide strong evidence that in addition to neurones, astrocytes also express binding sites for second messengers.
authors.affiliation: Department of Physiology, University of Basel, Switzerland.
authors.forename: E
authors.initials: E
authors.lastname: Hösli
authors.forename: L
authors.initials: L
authors.lastname: Hösli
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001345:Autoradiography; D001665:Binding Sites; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D005576:Colforsin / Q000378:metabolism*; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D009412:Nerve Fibers / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D009924:Organ Culture Techniques; D015240:Phorbol 12,13-Dibutyrate / Q000378:metabolism*; D011689:Purkinje Cells / Q000378:metabolism; D051381:Rats; D015290:Second Messenger Systems
chemical_list: D005576:Colforsin; D015240:Phorbol 12,13-Dibutyrate; D015544:Inositol 1,4,5-Trisphosphate