Determination of effector molecules in L-arabinose-induced bulge formation and lysis of Escherichia coli IFO 3545.
L-Ribulose 5-phosphate (L-Ru5P) was identified as the primary effector molecule of L-arabinose-induced bulge formation in Escherichia coli IFO 3545 observed in nutrient broth with 5% (w/v) sodium chloride. Hyperinduction of L-arabinose isomerase was due to exogenous sodium chloride and the resulting alteration in the balance of the L-arabinose-metabolizing enzymes resulted in accumulation of L-Ru5P. L-Ru5P induced the lysis of an L-arabinose-negative, L-Ru5P 4-epimerase-less mutant, ara-207, even when directly added to the medium but was not active against the wild-type strain. Some L-arabinose-utilizing (L-arabinose-resistant) revertants of ara-207 were still sensitive to L-Ru5P, indicating the involvement of another mutation in L-Ru5P-sensitivity other than genetic lack of L-Ru5P 4-epimerase. Among the various pentose phosphate esters tested, only L-Ru5P could induce lysis of ara-207. The lytic activity of L-Ru5P was attributed to its effect on bacterial sugar nucleotide metabolism which caused secondary accumulation of uridine 5'-diphosphate galactose (UDPGal), which provoked lysis induction.
Tanaka
T
T
Department of Biology, Faculty of Science, Osaka City University, Japan.
Muroi
H
H
Sunada
C
C
Taniguchi
M
M
Oi
S
S
D001089:Arabinose / Q000378:metabolism / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D012274:Ribulosephosphates / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014531:Uridine Diphosphate Galactose / Q000378:metabolism; D014882:Water-Electrolyte Balance
D012274:Ribulosephosphates; D014531:Uridine Diphosphate Galactose; C031524:ribulose 5-phosphate; D012965:Sodium Chloride; D001089:Arabinose