Human serum stimulates endothelin-1 synthesis more potently than prostacyclin production by cultured vascular endothelial cells.
Human serum stimulated the synthesis of a vasoconstrictive peptide, endothelin-1 (ET-1), and a vasodilatory prostanoid, prostacyclin (PGI2), by cultured human umbilical vein endothelial cells in a concentration- and time-dependent manner. Incubation in 20% concentration of the serum for 24 h stimulated ET-1 synthesis almost six-fold while PGI2 production increased two-fold. In addition, a tumor-promoting phorbol ester, phorbol 12-myristate 13-acetate (PMA), inhibited the serum-induced ET-1 production and stimulated PGI2 synthesis in a concentration- and time-dependent manner. Our results suggest that human serum derived factor(s) stimulate the production of vasoconstrictive ET-1 more potently than the synthesis of vasodilatory PGI2 by human vascular endothelial cells and that the production of these agents is differentially regulated by PMA.
Ristimäki
A
A
Children's Hospital, University of Helsinki, Finland.
Renkonen
R
R
Saijonmaa
O
O
Ylikorkala
O
O
Viinikka
L
L
D001769:Blood; D002478:Cells, Cultured; D016232:Endothelins / Q000096:biosynthesis*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D011464:Epoprostenol / Q000096:biosynthesis*; D006801:Humans; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D016232:Endothelins; D011464:Epoprostenol; D013755:Tetradecanoylphorbol Acetate