[Induction of differentiation of human T-cell leukemic cell line (CEM) by phorbol ester].
In the present study, we investigated the effect of phorbol 12-myristate 13-acetate (PMA) on induction of differentiation of human T-cell leukemic cell line (CEM). The results showed that uninduced cells were T1+, T4+, T9+, T10+, T3- and TcR-, representing thymus stage II differentiation. When culturing in vitro with PMA for 5 to 7 days, CEM cells expressed mature T-cell marker (T3) whereas the percentage of cells expressing proliferation-association antigen (T9) was markedly decreased. In addition, PMA could significantly inhibit the growth of CEM cells. We also found that after co-culture with PMA for 2 to 3 days, CEM cells showed distinct morphologic changes. These findings indicate that PMA may induce CEM cells to differentiate towards mature stage.
Yao
X R
XR
Institute of Clinical Medicine, China-Japan Friendship Hospital, Beijing.
D000911:Antibodies, Monoclonal / Q000032:analysis; D015704:CD4 Antigens / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000473:pathology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D013755:Tetradecanoylphorbol Acetate