[Prospects for using DNA probes for rapid diagnosis of antibiotic resistance in clinical strains of enteric bacteria].
Aminoglucoside resistance patterns of clinical strains of enteric bacteria isolated from inpatients of Moscow clinics were determined. APH(3')-I and AAC(3)-II were shown to be the most frequent. The aphA1 and aacC2 genes encoding the enzymes were cloned from the R plasmid of the transconjugant of the E. coli clinical strains. DNA probes based on the determined nucleotide sequences of the cloned genes were constructed and used in DNA-DNA hybridization experiments. The results on the occurrence of APH(3')-I and AAC(3)-II in the strains tested were confirmed by the DNA-DNA hybridization. Prospects for developing a set of DNA probes for rapid diagnosis of antibiotic resistance are discussed.
Vakulenko
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D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011815:R Factors / Q000235:genetics
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D015342:DNA Probes; D004269:DNA, Bacterial
Perspektivy ispol'zovaniia DNK-zondov dlia ékspress-diagnostiki antibiotikorezistentnosti klinicheskikh shtammov énterobakteriĭ.