Effect of insulin on the properties of liver carnitine palmitoyltransferase in the starved rat: assessment by the euglycemic hyperinsulinemic clamp.
The effect of insulin on the properties of liver carnitine palmitoyltransferase I (CPT I) was assessed in conscious starved rats with the euglycemic hyperinsulinemic clamp. A 24-hour clamp was necessary to fully reverse the effect of starvation on liver malonyl-CoA concentration, CPT I maximal activity, and apparent km and Ki for malonyl-CoA. Since glucagon was not decreased during the clamp, insulin is the major factor involved in the regulation of CPT I.
Pénicaud
L
L
Centre de Recherche sur la Nutrition, CNRS, Meudon-Bellevue, France.
Robin
D
D
Robin
P
P
Kandé
J
J
Picon
L
L
Girard
J
J
Ferré
P
P
D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism*; D005260:Female; D005934:Glucagon / Q000097:blood; D015309:Glucose Clamp Technique; D007328:Insulin / Q000097:blood / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008316:Malonyl Coenzyme A / Q000378:metabolism; D009994:Osmolar Concentration; D051381:Rats; D011919:Rats, Inbred Strains; D013217:Starvation / Q000378:metabolism*; D013997:Time Factors
D007328:Insulin; D008316:Malonyl Coenzyme A; D005934:Glucagon; D002334:Carnitine O-Palmitoyltransferase