Cloning, disruption and sequence of the gene encoding yeast C-5 sterol desaturase.
The ERG3 gene from Saccharomyces cerevisiae has been cloned by complementation of an erg3-2 mutation. ERG3 is the putative gene encoding the C-5 sterol desaturase required for ergosterol biosynthesis. The functional gene has been localized on a 2.5-kb HindIII-BamHI fragment containing an open reading frame comprising 365 amino acids. Gene disruption resulting from a deletion/substitution demonstrates that ERG3 is not essential for cell viability or the sparking function.
Arthington
B A
BA
Department of Biology, Indiana University-Purdue University at Indianapolis 46205.
Bennett
L G
LG
Skatrud
P L
PL
Guynn
C J
CJ
Barbuch
R J
RJ
Ulbright
C E
CE
Bard
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004875:Ergosterol / Q000096:biosynthesis*; D016368:Frameshift Mutation; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D013261:Sterols / Q000378:metabolism*
D013261:Sterols; D010088:Oxidoreductases; C039306:sterol delta-5 desaturase; D004875:Ergosterol