[Activation of trans-acting transcription factors in T-lymphocytes infected by the intracellular parasitic protozoan Theileria parva].
The intracellular protozoan parasite, Theileria parva, causes a lymphoproliferative disease of T-cells in cattle and uncontrolled lymphocyte proliferation in culture. We have identified and characterized in infected cells the trans-acting transcription factors NF-kappa B, AP1 and NF1 controlling lymphocyte-specific expression of several genes. Theileria infected lymphocytes contain constitutively high levels of active NF-kappa B in nuclear fraction. Factor AP1 had high levels of binding activity in nuclear fraction of these cells only in day 1 after passage. Then AP1 activity decreased rapidly in the course of culture growth. Simultaneously, we observed differential changes in the binding activity of several transcription factors of the NF1 family. Selective killing of the parasite, which leaves the host cells intact, results in a dramatic loss of the transcription factors NF-kappa B, AP1 and NF1 from the nuclear fraction of T-cells. At the same time, the activity of TATA-box binding proteins did not change significantly following the theilericidal drug treatment. We propose that high levels of activated transcription factors NF-kappa B, AP1 and NF1 are maintained by the presence of parasite in infected T-cells.
Ivanov
V N
VN
Villiams
R O
RO
D000818:Animals; D002417:Cattle; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism; D009841:Oligonucleotides / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000378:metabolism / Q000469:parasitology*; D013801:Theileriasis / Q000097:blood / Q000235:genetics*; D014157:Transcription Factors / Q000502:physiology*; D014357:Trypsin
D004268:DNA-Binding Proteins; D009841:Oligonucleotides; D014157:Transcription Factors; D014357:Trypsin
Aktivatsiia transdeĭstvuiushchikh faktorov transkriptsii v t-limfotsitakh, infitsirovannykh vnutrikletochnym paraziticheskim prosteĭshim Theileria parva.