Glucocorticoids "receptors" in human gingiva.
Specific binding of [3H]-dexamethasone by the cytoplasmic fraction of normal human gingiva was carried out. Different concentrations of [3H]-dexamethasone were used with or without cold dexamethasone to determine the binding. Binding data by Scatchard plot yielded a straight line indicating a single class of specific receptors with equilibrium dissociation constant of 1.39 x 10(-9) M and B max of 80 fmol/mg protein. The proteins satisfied the high affinity and low capacity requirements of receptor.
Suresh
R
R
Dept. of Biochemistry, Central Leather Research Institute, Adyar, Madras, India.
Dhar
S C
SC
D000293:Adolescent; D000818:Animals; D002458:Cell Fractionation; D003593:Cytoplasm / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D003907:Dexamethasone / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism; D005260:Female; D005881:Gingiva / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011869:Radioligand Assay; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism
D011965:Receptors, Glucocorticoid; D003907:Dexamethasone; D003851:Deoxyribonucleases; D012260:Ribonucleases; D010450:Endopeptidases