[Evaluation of new system for the detection of IgE antibodies (CAP) in atopic diseases].
An evaluation of a newly developed IgE antibody assay system (CAP) was carried out. There was a clear correlation between IgE antibody titers measured by CAP single and RAST (= 0.642 to 0.979). It turned out that CAP single is more sensitive than RAST and non-specific adsorption of IgE immunoglobulin to the solid phase was assumed to be less in CAP than in RAST. Pathogenic allergens diagnosed either clinically or by in vitro assay systems were compared. The sensitivity and specificity of the CAP system were 94.2% and 87.3%, respectively. CAP multi which binds groups of multiple allergens on the solid phase, was examined for the screening of hypersensitivity to categories of allergens. Statistical sensitivities of CAP multi resided between 63.9% to 86.2%, while the specificities were 98% to 100%, indicating that CAP multi is useful for the exploration of causative allergens. Phadiatop, which fixes multiple inhalant allergens, showed a sensitivity of 89.6%. The specificity of phadiatop was 93.9%, when examined for intrinsic bronchial asthma patients, and 91.2% for normal subjects indicating that phadiatop is more useful than total IgE measurement for the screening of atopic trait.
Okudaira
H
H
Department of Medicine, University of Tokyo.
Ito
K
K
Miyamoto
T
T
Wagatsuma
Y
Y
Matsuyama
R
R
Kobayashi
S
S
Nakazawa
T
T
Okuda
M
M
Otsuka
H
H
Baba
M
M
D000293:Adolescent; D000485:Allergens / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005069:Evaluation Studies as Topic; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D007118:Immunoassay / Q000379:methods* / Q000592:standards; D007073:Immunoglobulin E / Q000032:analysis*; D007223:Infant; D011852:Radioallergosorbent Test; D012882:Skin Tests
D000485:Allergens; D007073:Immunoglobulin E