Induction of acyl-CoA-binding protein and its mRNA in 3T3-L1 cells by insulin during preadipocyte-to-adipocyte differentiation.
The induction of acyl-CoA-binding protein (ACBP) and ACBP mRNA was investigated in 3T3-L1 cells during growth and insulin-induced differentiation. The level of ACBP relative to both total soluble protein and DNA increased during insulin-stimulated conversion of 3T3-L1 cells from preadipocytes into fully developed adipocytes. So did the total rate of lipogenesis, as measured by incorporation of [1-14C]acetate. A similar increase in ACBP mRNA relative to total RNA was observed. These results therefore suggest that ACBP plays a specific role in the lipogenic process. However, this role might be indirect, as the increase in lipogenesis preceded the increase in ACBP. The significance of this finding is discussed.
Hansen
H O
HO
Institute of Biochemistry, University of Odense, Denmark.
Andreasen
P H
PH
Mandrup
S
S
Kristiansen
K
K
Knudsen
J
J
D000085:Acetates / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology* / Q000502:physiology; D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003001:Cloning, Molecular; D003907:Dexamethasone / Q000494:pharmacology; D026261:Diazepam Binding Inhibitor; D015723:Gene Library; D007700:Kinetics; D008055:Lipids / Q000096:biosynthesis; D008099:Liver / Q000378:metabolism; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000085:Acetates; D002352:Carrier Proteins; D026261:Diazepam Binding Inhibitor; D008055:Lipids; D012333:RNA, Messenger; D003907:Dexamethasone