Nucleosomal organization of chromatin in sperm nuclei of the bivalve mollusc Aulacomya ater.
The sperm nuclei of Aulacomya ater, family Mitylidae, contain three proteins (X, Aa5 and Aa6) which are specific to this cell type coexisting with a set of five somatic-type histones. Information about the chromatin structure resulting from this kind of association is scarce. Therefore, we have probed the structure of this sperm chromatin through digestion with micrococcal nuclease in combination with salt fractionation. The data obtained have allowed us to propose a nucleosomal arrangement for this chromatin. However, two types of nucleosomes would be present in agreement with their protein components.
Olivares
C
C
Laboratory of Developmental Biology, Universidad de Talca, Chile.
Ruiz
S
S
D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure*; D002843:Chromatin / Q000737:chemistry / Q000648:ultrastructure*; D008297:Male; D008836:Micrococcal Nuclease; D008974:Mollusca; D009687:Nuclear Proteins / Q000737:chemistry*; D012965:Sodium Chloride; D013094:Spermatozoa / Q000737:chemistry*
D002843:Chromatin; D009687:Nuclear Proteins; D012965:Sodium Chloride; D008836:Micrococcal Nuclease