title: Shaw-like rat brain potassium channel cDNA's with divergent 3' ends.
abstract: The complete amino acid sequence of a potassium channel protein of rat brain, Kv3.2b, plus a partial sequence of a related channel, Kv3.2c, are deduced from molecular cloning of the respective cDNA's. Kv3.2b and Kv3.2c share extensive amino acid sequence identity with a previously identified channel, RKShIIIA[1], before diverging to unique carboxy termini. Probes specific for Kv3.2b and RKShIIIA detect similarly sized mRNA's on Northern blots. These two proteins are encoded by a single gene based on genomic Southern blotting, and therefore arise by alternative splicing. In vitro transcribed mRNA for Kv3.2b induces the expression of outward K+ currents in Xenopus oocytes under voltage-clamp conditions.
authors.affiliation: Department of Pharmacology, Merck, Sharp and Dohme Research Laboratories, West Point, PA 19486.
authors.forename: C
authors.initials: C
authors.lastname: Luneau
authors.forename: R
authors.initials: R
authors.lastname: Wiedmann
authors.forename: J S
authors.initials: JS
authors.lastname: Smith
authors.forename: J B
authors.initials: JB
authors.lastname: Williams
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D004330:Drosophila / Q000235:genetics; D008564:Membrane Potentials / Q000502:physiology; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D015221:Potassium Channels / Q000737:chemistry / Q000235:genetics*; D012326:RNA Splicing / Q000502:physiology*; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014981:Xenopus / Q000378:metabolism
chemical_list: D015221:Potassium Channels