Hybrid formation of rabbit and rat hepatic glutathione S-transferases.
1. A reconstitution experiment resulted in the formation of new proteins between limited combinations of rat and rabbit hepatic glutathione S-transferases: AA(subunit composition: YcYc) and R3b(Y3Y3), Lig(YaYa) and R3b(Y3Y3), and A(Yb1Yb1) and R2(Y2Y2). 2. It was demonstrated that the new protein formed between R2 and A had the subunit composition of Y2Yb1, suggesting a hybrid of rabbit (R2) and rat isozyme (A). 3. This hybrid protein showed intermediate spec. acts between those of R2 and A when either 1-chloro-2,4-dinitrobenzene (CDNB) or 1,2-dichloro-4-nitrobenzene (DCNB) was employed as the substrate.
Miyaura
S
S
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Sasaki
H
H
Isono
H
H
D000818:Animals; D005260:Female; D005982:Glutathione Transferase / Q000737:chemistry* / Q000378:metabolism; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000737:chemistry*; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008297:Male; D055503:Protein Multimerization; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
D007527:Isoenzymes; D046911:Macromolecular Substances; D005982:Glutathione Transferase