title: Temperature cycler evaluation: what do you need to know?
abstract: Which of the 20 different, commercially available, temperature cyclers adequately fulfill all functional requirements? How can an investigator analyze instrument function? This report describes in detail, for the first time, the characteristics of Ericomp temperature cyclers. The data show that the temperature uniformity, accuracy and maximum overshoot is +/- 0.34 degree C, less than 0.5 degree C and 0.17 degree C, respectively. Cycling temperature reproducibility is 0.1%, and cycling time reproducibility is 0.4%. These data compare very favourably to published data describing other temperature cyclers. The techniques used to obtain these data serve as a model for evaluating instruments for well-to-well uniformity, temperature accuracy and within-run reproducibility.
authors.affiliation: Ericomp, Inc., San Diego, CA 92121.
authors.forename: G P
authors.initials: GP
authors.lastname: Tweed
authors.forename: J
authors.initials: J
authors.lastname: Whitney
authors.forename: P L
authors.initials: PL
authors.lastname: Bloch
mesh_terms: D001709:Biotechnology / Q000295:instrumentation*; D005069:Evaluation Studies as Topic; D015203:Reproducibility of Results; D013696:Temperature; D013997:Time Factors; D014867:Water
chemical_list: D014867:Water