Overexpression of the actin gene is associated with the morphogenesis of Candida albicans.
A progressive increase in the synthesis of actin mRNA was observed by Northern blot analysis, when cells were induced to form germ tubes at 37 degrees C by N-acetyl-D-glucosamine. Presence of trifluoperazine, a calmodulin inhibitor, or incubation of cells at 25 degrees C, or by replacing N-acetyl-D-glucosamine with glucose which inhibited germ tube formation lowered this synthesis. Furthermore, in vitro translation of total RNA revealed an increase in the synthesis of actin (45 kDa) during germ tube formation. These results suggest for the first time that the expression of actin gene is regulated during morphogenesis of C. albicans.
Paranjape
V
V
Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Datta
A
A
D000117:Acetylglucosamine / Q000494:pharmacology; D000199:Actins / Q000235:genetics*; D015152:Blotting, Northern; D002176:Candida albicans / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D009024:Morphogenesis / Q000187:drug effects; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology; D013696:Temperature; D013997:Time Factors; D014268:Trifluoperazine / Q000494:pharmacology
D000199:Actins; D012333:RNA, Messenger; D014268:Trifluoperazine; D005947:Glucose; D000117:Acetylglucosamine