P2 purinoceptor regulation of mucin release by airway goblet cells in primary culture.
1. The effects of adenine analogues on mucin release by airway goblet cells have been examined in a hamster primary tracheal epithelial cell culture. 2. Adenosine, a P1 receptor agonist, had no effect on mucin release even at 2 mM, while ATP, a P2 receptor agonist, stimulated mucin release in a dose-dependent fashion with an apparent EC50 of 20 microM. The relative potency order among adenine nucleotides was ATP greater than ADP greater than AMP = adenosine. 3. ATP gamma S, a non-hydrolyzable analogue of ATP, was equipotent with ATP in stimulating mucin release. The potency order among some ATP analogues was ATP greater than 2-methylthio ATP greater than alpha, beta-methylene ATP greater than beta, gamma-methylene ATP. Reactive blue 2, a putative P2 gamma-purinoceptor antagonist, did not block the ATP-induced mucin release. 4. The present results indicate that mucin release by airway goblet cells is stimulated by extracellular ATP via P2 receptor-mediated mechanism. We suggest that this mechanism may be important in the physiological regulation of airway goblet cell mucin release in vivo.
Kim
K C
KC
Department of Pharmacology and Toxicology, School of Pharmacy, University of Maryland, Baltimore 21201.
Lee
B C
BC
D000227:Adenine Nucleotides / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008647:Mesocricetus; D009077:Mucins / Q000378:metabolism*; D011983:Receptors, Purinergic / Q000187:drug effects*; D012137:Respiratory System / Q000166:cytology / Q000378:metabolism*; D014132:Trachea / Q000166:cytology / Q000378:metabolism; D014227:Triazines / Q000494:pharmacology
D000227:Adenine Nucleotides; D009077:Mucins; D011983:Receptors, Purinergic; D014227:Triazines; C012842:Cibacron Blue F 3GA; D008274:Magnesium