title: Automated purification of synthetic oligonucleotides.
abstract: We have automated the trityl-on purification of oligonucleotides by use of an XYZ axis robotic solid-phase extraction system. This greatly decreased the preparation time required for oligonucleotide purification. After about 15 min for set up of the samples and instrument, the oligonucleotides are automatically purified with a 15-min run time per sample. Thus, for example, the purification of 15 oligonucleotides requires only about 15 min of preparation time and 4 h of machine time. Yields and purity are equivalent to manual methods.
authors.affiliation: University of California, San Francisco 94143-0448.
authors.forename: K M
authors.initials: KM
authors.lastname: Ivanetich
authors.forename: J
authors.initials: J
authors.lastname: Akiyama
authors.forename: D V
authors.initials: DV
authors.lastname: Santi
authors.forename: M
authors.initials: M
authors.lastname: Reschenberg
mesh_terms: D000327:Adsorption; D002845:Chromatography / Q000295:instrumentation*; D002851:Chromatography, High Pressure Liquid; D008722:Methods; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000302:isolation & purification*; D012083:Renin; D014320:Triphenylmethyl Compounds
chemical_list: D009841:Oligonucleotides; D014320:Triphenylmethyl Compounds; D012083:Renin