Characterization of gingival epithelium epidermal growth factor receptor.
The binding characteristics of gingival epithelium epidermal growth factor (EGF) receptor were investigated using epithelial cell membranes from bovine gingiva. The binding of [125I]EGF was found to be time and protein concentration dependent, reversible, and specific. Unlabeled EGF competed for [125I]EGF binding with IC50 of 0.25nM and maximum displacement of 93% at 0.81nM. Scatchard analysis of the binding data inferred the presence of two binding sites, one of high affinity (Kd = 3.3 nM and Bmax = 47.3fmol/mg protein) and the other of a low affinity (Kd = 1.6 microM and Bmax = 1.9pmol/mg protein). Crosslinking of [125I]EGF to gingival membranes followed by polyacrylamide gel electrophoresis and autoradiography revealed a receptor protein of 170kDa.
Sengupta
S
S
Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
Liu
J
J
Wang
S L
SL
Piotrowski
E
E
Slomiany
A
A
Slomiany
B L
BL
D000818:Animals; D001345:Autoradiography; D001667:Binding, Competitive; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004815:Epidermal Growth Factor / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism*; D005881:Gingiva / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D011869:Radioligand Assay
D007328:Insulin; D004815:Epidermal Growth Factor; D066246:ErbB Receptors