Synthesis of a fluorescent derivative of cyclosporin A for high-performance liquid chromatography analysis.
A direct assay method for use in studies of cyclosporin binding must be highly sensitive and selective since it must be capable of measuring the concentrations encountered in the protein-free matrix. The failure of current HPLC methods to achieve the sensitivity required for binding studies may be attributed to the use of UV detection, which relies on the relatively weak end-absorption of cyclosporin A. A method involving fluorescence derivatization was sought with the aim of increasing HPLC assay sensitivity. A method is described for producing a fluorescent derivative of cyclosporin A, a compound which has no functional groups which are easily derivatized. However, intramolecular rearrangement of cyclosporin A to form its structural isomer, isocyclosporin A, exposes a secondary amine which can be reacted with dansyl chloride to produce a fluorescent derivative. This two-step derivatization procedure was used as the basis of an HPLC fluorescence assay. Although this assay is not sufficiently sensitive to measure concentrations encountered in the protein-free matrix during plasma binding studies, the method does point to the possible development of a more sensitive assay using a derivatizing reagent other than dansyl chloride.
Fois
R A
RA
Pharmacy Department, University of Sydney, N.S.W., Australia.
Ashley
J J
JJ
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003524:Cyclosporins / Q000032:analysis / Q000138:chemical synthesis*; D004355:Drug Stability; D005453:Fluorescence; D007700:Kinetics; D008969:Molecular Sequence Data
D003524:Cyclosporins; C070227:dansylisocyclosporin A; C070226:isocyclosporin A