[Detection of influenza virus nucleoprotein on the surface of infected cells and in free nonvirion form].
The inner viral nucleoprotein synthesized de novo is shown to be exposed on the surface of the chicken embryo infected with influenza virus. The kinetics of the nucleoprotein located on the surface does not correlate with the kinetics of cell destruction. In culture or allantois virus containing liquids the large number of extracellular viral nucleoprotein prone to antinucleoprotein monoclonal antibodies was found. The accumulation of this nucleoprotein occurs in the period when cell destruction is absent, it is eliminated by the adsorption of the virus on erythrocytes or centrifugation at 70,000 g for 2 hours (20%) or by centrifugation at 10,000 g for 4 hours (50%).
Semenova
N P
NP
Prokudina
E N
EN
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002478:Cells, Cultured; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D009980:Influenza A virus / Q000276:immunology / Q000378:metabolism*; D007700:Kinetics; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000032:analysis* / Q000276:immunology; D016601:RNA-Binding Proteins; D011863:Radioimmunoassay; D015531:Radioimmunoprecipitation Assay; D014758:Viral Core Proteins / Q000032:analysis* / Q000276:immunology; D014771:Virion / Q000378:metabolism
D000911:Antibodies, Monoclonal; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins
Vyiavlenie nukleoproteina virusa grippa na poverkhnosti zarazhennykh kletok i v svobodnoĭ vnevirionnoĭ forme.