title: Distribution and phylogenetic significance of 27-hydroxy-octacosanoic acid in lipopolysaccharides from bacteria belonging to the alpha-2 subgroup of Proteobacteria.
abstract: Lipopolysaccharides (LPSs) from a number of bacteria belonging to the alpha-2 subgroup of the class Proteobacteria were screened for the presence of 27-hydroxy-octacosanoic acid (27-OH-28:0). With few exceptions, most of the bacteria contained 27-OH-28:0 in their lipid A fractions. In addition, some of the bacteria contained other n-2-hydroxylated long-chain fatty acids hitherto not reported. The distribution of 27-OH-28:0 was restricted to the alpha-2 subgroup. LPSs from members of the other subgroups (the beta and gamma subgroups), including some well-characterized enterobacterial LPSs, were devoid of 27-OH-28:0. Our results indicate that the presence of n-2-hydroxylated long-chain fatty acids in LPSs might be used as a chemophylogenetical marker.
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, Athens 30602.
authors.forename: U R
authors.initials: UR
authors.lastname: Bhat
authors.forename: R W
authors.initials: RW
authors.lastname: Carlson
authors.forename: M
authors.initials: M
authors.lastname: Busch
authors.forename: H
authors.initials: H
authors.lastname: Mayer
mesh_terms: D006880:Hydroxy Acids / Q000032:analysis*; D008070:Lipopolysaccharides / Q000032:analysis*; D013058:Mass Spectrometry; D015394:Molecular Structure; D010802:Phylogeny; D012230:Rhizobiaceae / Q000032:analysis* / Q000145:classification
chemical_list: D006880:Hydroxy Acids; D008070:Lipopolysaccharides; C059845:27-hydroxyoctacosanoic acid