Inhibition of proliferation of human immunodeficiency virus type 1 by novel heteropolyoxotungstates in vitro.
Fifteen heteropolyoxotungstates were tested for their effects on the proliferation of human immunodeficiency virus type 1 (HIV-1) using an in vitro system consisting of MT-4 cells and HTLV-IIIb. Eight heteropolyoxotungstates (HPOTs) with the Keggin structure or dimerized deficient Keggin structure proved to be potent inhibitors of HIV-1. In contrast, seven non-Keggin HPOTs including HPA 23 did not have significant effects on HIV-1 proliferation at non-toxic doses. [PTi2W10O40]7- (PM-19) was the most potent inhibitor of HIV-1 among the 15 HPOTs tested. The inhibition of HIV-1 replication by PM-19 presumably results from impaired virus adsorption and/or penetration into target cells. Viral spread of HIV-1 and HIV-2 on cell-to-cell basis was also susceptible to PM-19. In combination, PM-19 and 3'-azido-3'-deoxythymidine were synergistic in inhibiting HIV-1 proliferation.
Take
Y
Y
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Japan.
Tokutake
Y
Y
Inouye
Y
Y
Yoshida
T
T
Yamamoto
A
A
Yamase
T
T
Nakamura
S
S
D000327:Adsorption / Q000187:drug effects; D002461:Cell Line, Transformed; D016264:Dextran Sulfate / Q000494:pharmacology; D004357:Drug Synergism; D005455:Fluorescent Antibody Technique; D015726:Giant Cells / Q000187:drug effects; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008982:Molybdenum / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology; D014414:Tungsten / Q000494:pharmacology*; D017973:Tungsten Compounds; D014779:Virus Replication / Q000187:drug effects; D015215:Zidovudine / Q000494:pharmacology
D011108:Polymers; D017973:Tungsten Compounds; D015215:Zidovudine; D008982:Molybdenum; C064098:polyoxotungstate PM 19; D016264:Dextran Sulfate; D014414:Tungsten