Inhibition of 5S RNA transcription in vitro by nucleosome cores with low or high levels of histone acetylation.
Nucleosomes exert strong inhibitory effects on gene transcription in vitro and in vivo. Since most DNA is packaged in nucleosomes, there must exist mechanisms to alleviate this inhibition during gene activation. Nucleosomes could be destabilized by histone acetylation which is strongly correlated with gene expression. We have compared the effects of nucleosomes cores with low or high levels of histone acetylation on 5S RNA transcription with Xenopus nuclear extracts in vitro. Little or no difference was observed over a range of 1 to 15 nucleosome cores per plasmid template. This result suggests that nucleosomal DNA is not more accessible to transcription factors and to the transcription machinery in acetylated nucleosomes.
Roberge
M
M
Department of Biological Chemistry, Faculty of Medicine, University of California, Davis 95616.
O'Neill
T E
TE
Bradbury
E M
EM
D000107:Acetylation; D000818:Animals; D004275:DNA, Ribosomal / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D006657:Histones / Q000378:metabolism*; D009707:Nucleosomes / Q000378:metabolism*; D010957:Plasmids / Q000502:physiology; D012341:RNA, Ribosomal, 5S / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology; D015533:Transcriptional Activation; D014982:Xenopus laevis / Q000235:genetics
D004275:DNA, Ribosomal; D006657:Histones; D009707:Nucleosomes; D012341:RNA, Ribosomal, 5S