[Influenza virus proteins: preparation of a soluble M1 polypeptide by means of a stepwise deproteination of virions].
Layer by layer uncoating of influenza A and B viruses with non-ionic detergent (NP-40) at fixed pH was developed. Treatment of virions with NP-40 at neutral or alkaline pH solubilized the lipoprotein envelope and the surface glycopolypeptides HA1 and HA2, but the internal core structures containing matrix protein M1 remained. Exposition of the cores in acidic media (pH 4,5 and lower) selectively solubilized protein M1 and released viral ribonucleoprotein (RNP). The resulting M1 sedimented in a glycerol gradient with a coefficient of 2.8 S and most probably exists as a monomer of 27,000 Da polypeptide. Neutralization of protein M1 with Tris-HC1 at pH 7.0 did not cause aggregation of M1 polypeptides. The described method of viron layer by layer uncoating with non-ionic detergent at fixed pH is suitable for isolation of subvirus structures and individual viral proteins.
Zhirnov
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D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D009980:Influenza A virus / Q000378:metabolism*; D009981:Influenza B virus / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000302:isolation & purification*; D014771:Virion / Q000378:metabolism*
D003902:Detergents; C031540:M-protein, influenza virus; C488936:M1 protein, Influenza A virus; D012261:Ribonucleoproteins; D014763:Viral Matrix Proteins
Belki virusa grippa: Poluchenie rastvorimogo polipeptida M1 postredstvom poétapnoĭ deproteinizatsii virionov.