Isolation of histone-like proteins from mitochondria of bovine heart.
Two methods for isolating and purifying histone-like proteins from mitochondria of bovine heart are described. In the first, a sonicated extract of the mitochondria was fractionated in three chromatography steps, including affinity chromatography on DNA-cellulose, to purify a protein that resembles very closely the histone-like protein (HM) of yeast mitochondria. In the second method, an acid extract of the heart mitochondria was the starting material; two other histone-like proteins were separated. Thus, as in mitochondria of Xenopus laevis, several histone-like proteins are present in mitochondria of bovine heart.
Yamada
E W
EW
Department of Biochemistry and Molecular Biology, University of Manitoba, Winnipeg, Canada.
Dotzlaw
H
H
Huzel
N J
NJ
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001426:Bacterial Proteins / Q000302:isolation & purification; D002417:Cattle; D002845:Chromatography / Q000379:methods; D004268:DNA-Binding Proteins / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000302:isolation & purification*; D008929:Mitochondria, Heart / Q000737:chemistry*; D013464:Sulfuric Acids
D000596:Amino Acids; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D006657:Histones; D013464:Sulfuric Acids; C040330:histone-like protein HU, bacteria; C033158:sulfuric acid