Biodegradation potential of some micromycetes for pentachlorophenol.
A first screening was performed upon 100 strains of micromycetes cultivated on solid media (malt extract medium and mineral medium) with pentachlorophenol (PCP) (0.5 g/liter). Under these conditions, 50 strains gave a light blurring around the inoculation spot, indicating some PCP degradation. Later, 50 selected strains were cultivated in liquid synthetic medium with PCP (1 g/liter). After 6 days of cultivation, photodegradation occurred for 25%. On the whole, the consumption of PCP was 25% for Zygomycetes, 3% for yeasts, and 10-15% for Deuteromycetes, except 7% for Tuberculariales. It was shown that glucose repressed the PCP consumption. Among degrading fungi, some could grow with PCP when cultures were initiated with spores, others could not. A more detailed study was done with Phoma glomerata cultivated in liquid synthetic medium (PCP 100 mg/liter) in darkness or with light. Photodegradation increased up to 25% but abiotic degradation occurred also in darkness (8%). Consumption of PCP by Ph. glomerata was 27% after 2 days with light and was lower in darkness (19%).
Seigle-Murandi
F
F
Laboratoire de Botanique, Cryptogamie, Biologie Cellulaire, et Génétique, UFR de Pharmacie, Université Joseph Fourier (Grenoble I), Meylan, France.
Steiman
R
R
Benoit-Guyod
J L
JL
D001673:Biodegradation, Environmental; D005658:Fungi / Q000378:metabolism*; D007700:Kinetics; D010416:Pentachlorophenol / Q000378:metabolism*; D013045:Species Specificity; D013997:Time Factors
D010416:Pentachlorophenol