A rapid method to determine the isotype and specificity of coproantibodies in mice infected with Trichinella or fed cholera toxin.
A method was developed to isolate and test coproantibodies from mice infected with Trichinella or mice fed cholera toxin. It was rapid as results were obtained within 3 h, and it was applicable to a single faecal pellet. It reduced the number of experimental animals required 7-fold, and reduced variability, since samples were obtained from the same experimental animal over time. As it was noninvasive, it did not alter normal gut physiology, nor the course of the parasitic infection. The immune response was monitored over time in individual mice, and specific sIgA and IgG isotypes were detected for both Trichinella and cholera toxin by ELISA.
deVos
T
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Department of Zoology, University of Manitoba, Winnipeg, Canada.
Dick
T A
TA
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000909:Antibodies, Helminth / Q000032:analysis; D000918:Antibody Specificity; D002772:Cholera Toxin / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000737:chemistry*; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D007132:Immunoglobulin Isotypes / Q000032:analysis*; D051379:Mice; D014235:Trichinellosis / Q000276:immunology*
D000906:Antibodies; D000909:Antibodies, Helminth; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D002772:Cholera Toxin