Lipopolysaccharide-instructed, cryopreserved, human monocytes sequentially convert plasma fibrinogen to fibrin and lyse the fibrin formed.
Cryopreserved human monocytes have been examined for their procoagulant and profibrinolytic capacities on lipopolysaccharide (LPS) stimulation, when given the possibility to act upon fibrinogen in heparinized plasma. As shown by the appearance of fibrinopeptide A, indicating thrombin action on the fibrinogen molecule, and by the appearance of D-Dimer, indicating the action of plasmin on fibrin, it is apparent that LPS-instructed monocytes give rise to fibrin which is subsequently lysed. Thus, delineation (fibrin formation) appears to be followed by fibrin removal (restitution).
Kierulf
P
P
Clinical Chemistry Department, Ullevaal University Hospital, Oslo, Norway.
Andersen
A B
AB
D001779:Blood Coagulation Factors / Q000378:metabolism; D015925:Cryopreservation; D005337:Fibrin / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism; D005342:Fibrinolysis; D006801:Humans; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D010960:Plasminogen Activators / Q000378:metabolism
D001779:Blood Coagulation Factors; D008070:Lipopolysaccharides; C045072:macrophage procoagulant activity; D005337:Fibrin; D005340:Fibrinogen; D010960:Plasminogen Activators