Sequence and expression in Escherichia coli of a Mycoplasma hominis gene encoding elongation factor Tu.
We describe the molecular cloning and the complete nucleotide (nt) sequence of a Mycoplasma hominis gene common to a broad range of Mycoplasma species, as defined by hybridization analysis with the cloned gene. Production of M. hominis protein in Escherichia coli was assayed by use of a monoclonal antibody. The nt sequence analysis revealed a 1194-bp open reading frame that could encode a 43 516-Da protein. Computer-aided sequence comparison indicated that the gene codes for elongation factor Tu.
Lüneberg
E
E
Institut für Medizinische Mikrobiologie, Medizinische Hochschule, Hannover, F.R.G.
Kamla
V
V
Hadding
U
U
Frosch
M
M
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D014644:Genetic Variation; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009174:Mycoplasma / Q000235:genetics*; D010444:Peptide Elongation Factor Tu / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D011994:Recombinant Proteins; D010444:Peptide Elongation Factor Tu