title: [Dependence of human erythrocyte methemoglobin reductase on temperature].
abstract: The effect of temperature on the activities of cytoplasmic and membrane-bound fractions of NADH-cytochrome beta 5 reductase on the total activity of methemoglobin reductase in intact human erythrocytes was studied within the temperature range of 20-50 degrees C. The above three activities showed a break in the Arrhenius plots at 42 degrees C which was attributed to irreversible inactivation of the enzymes. Thermal inactivation of methemoglobin reductase in erythrocytes was found to increase the methemoglobin content concomitantly with a decrease in the osmotic stability and activation of spontaneous cell hemolysis.
authors.forename: N M
authors.initials: NM
authors.lastname: Kozlova
authors.forename: E A
authors.initials: EA
authors.lastname: Chernitskiĭ
mesh_terms: D003579:Cytochrome Reductases / Q000378:metabolism; D042966:Cytochrome-B(5) Reductase / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000201:enzymology; D004912:Erythrocytes / Q000201:enzymology*; D006454:Hemoglobins / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D008706:Methemoglobin / Q000378:metabolism; D013696:Temperature
chemical_list: D006454:Hemoglobins; D008706:Methemoglobin; D003579:Cytochrome Reductases; D042966:Cytochrome-B(5) Reductase
vernacular_title: Zavisimost' aktivnost' metgemoglobinreduktazy éritrotsitov cheloveka ot temperatury.