title: O(6)-methylguanine-DNA methyltransferase and uracil DNA glycosylase in human broncho-alveolar lavage cells and peripheral blood mononuclear cells from tobacco smokers and non-smokers.
abstract: Because interindividual variations in the activities of DNA repair enzymes may be a risk factor in the pathogenesis of lung diseases, O(6)-methylguanine-DNA methyltransferase (O(6)-MT) and uracil DNA glycosylase (UDG) were measured in broncho-alveolar lavage cell (BALC) and peripheral blood mononuclear cell (PBM) samples from 57 healthy volunteers (25 smokers and 32 non-smokers). According to cotinine determination in 39 cases where serum for this was available, 38% of the self-acclaimed non-smokers had greater than 10 ng/ml of cotinine in their serum. Whether grouped into smokers and non-smokers according to clinical history or by serum cotinine, there were no statistically significant differences between these groups in O(6)-MT or UDG in either of the cell types. However, a tendency towards lower values in smokers was seen. The highest intraindividual variation in O(6)-MT activity was 7-fold, while the highest interindividual variation reached 18-fold. For UDG, the respective values were 24- and 307-fold. Although the distribution of O(6)-MT in BALC was different from that in PBM, the data are consistent with unimodality in both of the cell types. These findings suggest that exposure to cigarette smoke is not entirely responsible for the wide interindividual variation in O(6)-MT and UDG DNA repair activities.
authors.affiliation: Laboratory of Human Carcinogenesis, National Cancer Institute, NIH, Bethesda, MD 20892.
authors.forename: K
authors.initials: K
authors.lastname: Vähäkangas
authors.forename: G E
authors.initials: GE
authors.lastname: Trivers
authors.forename: S
authors.initials: S
authors.lastname: Plummer
authors.forename: R B
authors.initials: RB
authors.lastname: Hayes
authors.forename: H
authors.initials: H
authors.lastname: Krokan
authors.forename: M
authors.initials: M
authors.lastname: Rowe
authors.forename: R P
authors.initials: RP
authors.lastname: Swartz
authors.forename: H
authors.initials: H
authors.lastname: Yeager
authors.forename: C C
authors.initials: CC
authors.lastname: Harris
mesh_terms: D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000201:enzymology*; D045647:DNA Glycosylases; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D008297:Male; D008780:Methyltransferases / Q000032:analysis*; D009699:N-Glycosyl Hydrolases / Q000032:analysis*; D019853:O(6)-Methylguanine-DNA Methyltransferase; D012907:Smoking / Q000097:blood / Q000378:metabolism; D051981:Uracil-DNA Glycosidase
chemical_list: D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase