An enzyme immunoassay for human defensins.
We developed and optimized an enzyme immunoassay for human neutrophil defensins, cationic cysteine-rich peptides that participate in host defense and inflammation. The assay utilizes a sandwich design with a monoclonal capture antibody and a biotinylated monoclonal detecting antibody. Cetrimonium bromide is employed to obviate non-specific binding of defensins to surfaces. The assay has a sensitivity of 0.04-0.05 ng/ml and a working range of 0.05-10 ng/ml.
Panyutich
A V
AV
Will Rogers Pulmonary Research Laboratory, School of Medicine, University of California, Los Angeles 90024-1736.
Voitenok
N N
NN
Lehrer
R I
RI
Ganz
T
T
D000911:Antibodies, Monoclonal; D001770:Blood Bactericidal Activity; D001798:Blood Proteins / Q000032:analysis* / Q000276:immunology; D023082:Defensins; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D009504:Neutrophils / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D023082:Defensins