Hepoxilin A3 induces heat shock protein (HSP72) expression in human neutrophils.
In this paper we show that hepoxilin A3 induces the expression of heat shock protein expression in human neutrophils at a concentration of 100 nM using Western blotting techniques employing the use of a commercial monoclonal antibody to HSP72. No regiospecificity was observed as the 8S enantiomer of HxA3 was as active as the 8R enantiomer of HxA3. Comparison of the effects of HxA3 with 12S-HETE and PGA1 indicated that HxA3 was as effective as 12S-HETE although PGA1 was essentially inactive at the same concentration used for these 12-lipoxygenase products.
Lin
Z
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Research Institute, Hospital for Sick Children, Toronto, Canada.
Laneuville
O
O
Pace-Asciak
C R
CR
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D015126:8,11,14-Eicosatrienoic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D003600:Cytosol / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000097:blood*; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D011454:Prostaglandins A / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D006360:Heat-Shock Proteins; D006893:Hydroxyeicosatetraenoic Acids; D011454:Prostaglandins A; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; C038243:8-hydroxy-11,12-epoxyeicosa-5,9,14-trienoic acid; D015126:8,11,14-Eicosatrienoic Acid; C100573:prostaglandin A1