title: The nucleotide sequence of the infectious cloned DNA components of potato yellow mosaic virus.
abstract: The complete nucleotide sequence of a Venezuelan isolate of potato yellow mosaic virus (PYMV) has been determined, showing it to be typical of subgroup I geminiviruses in that it is whitefly-transmitted, has a circular, bipartite ssDNA genome and possesses bidirectionally orientated open reading frames (ORFs). The two genomic components have little sequence similarity apart from a common region of 268 nucleotides (nt) which is almost identical. Analysis of ORFs revealed six potential coding regions encoding proteins of Mr greater than 10K, four in PYMV A (2593 nt) and two in PYMV B (2547 nt), which are preceded by regulatory transcription elements and have polyadenylation signals present at the ends. Amino acid sequence alignments of PYMV DNA ORF-encoded proteins with those encoded by other previously sequenced geminivirus ORFs show that PYMV is closely related to those geminiviruses isolated from the New World, especially in the putative coat protein gene regions.
authors.affiliation: Department of Biology, Imperial College of Science, Technology and Medicine, U.K.
authors.forename: R H
authors.initials: RH
authors.lastname: Coutts
authors.forename: R S
authors.initials: RS
authors.lastname: Coffin
authors.forename: E J
authors.initials: EJ
authors.lastname: Roberts
authors.forename: W D
authors.initials: WD
authors.lastname: Hamilton
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry / Q000235:genetics; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry*; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics*; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D012091:Repetitive Sequences, Nucleic Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins