Glycine-like immunoreactivity in synaptic boutons of identified inhibitory interneurons in the mammalian spinal cord.
Glycine is thought to be a major inhibitory neurotransmitter in the mammalian CNS. Two types of physiologically identified interneurons, Renshaw cells and Ia inhibitory interneurons, were intracellularly stained with horseradish peroxidase, and their axon terminals were studied at the electron microscopic level. Post-embedding immunogold procedures were used to reveal the presence of glycine-like immunoreactivity. The synaptic terminals of both types of interneuron were significantly enriched with glycine-like immunoreactivity, providing support for the idea that glycine is a mediator of synaptic transmission in the recurrent and reciprocal inhibitory pathways to motoneurons.
Fyffe
R E
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Department of Physiology, University of North Carolina, Chapel Hill 27599-7545.
D000818:Animals; D000918:Antibody Specificity; D003429:Cross Reactions; D005998:Glycine / Q000276:immunology / Q000494:pharmacology*; D006735:Horseradish Peroxidase; D007395:Interneurons / Q000166:cytology* / Q000187:drug effects; D008839:Microelectrodes; D051381:Rats; D013116:Spinal Cord / Q000166:cytology* / Q000187:drug effects
D006735:Horseradish Peroxidase; D005998:Glycine