Determination of clenbuterol in urine as its cyclic boronate derivative by gas chromatography-mass spectrometry.
A rapid and reliable gas chromatographic-mass spectrometric method for the determination of clenbuterol in urine is described. Penbutolol was used as internal standard. Four derivatization procedures have been tested, of which 1-butaneboronic acid gave the best results. The method includes extraction of the alkalinized urine (3 ml) with tert.-butyl methyl ether-n-butanol (9:1), derivatization with 1-butaneboronic acid (15 min at room temperature), and analysis in the selected-ion monitoring mode of the derivatives of clenbuterol at m/z 243, 327 and 342 and of penbutolol at m/z 342 and 357. The detection limit is 0.5 ng/ml and the recovery better than 90%.
Polettini
A
A
Institute of Legal Medicine, University of Pavia, Italy.
Ricossa
M C
MC
Groppi
A
A
Montagna
M
M
D001897:Boronic Acids / Q000378:metabolism*; D002976:Clenbuterol / Q000378:metabolism / Q000652:urine*; D004300:Doping in Sports; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D015813:Substance Abuse Detection
D001897:Boronic Acids; D002976:Clenbuterol