[Purification of cytochrome B-561 from chromaffin granules and its physico-chemical properties].
A soft method of purification of cytochrome-561 from the membranes of chromaffin granules has been developed. It permits isolating a protein in its natural microsurroundings, i.e. a complex with lipids, provided that a buffer with high ionic force is used without a detergent. This method helps obtaining an electrophoretically homogeneous preparation as a high-molecular lipoprotein hexamer whose molecular weight is about 400 kDa. Basic physicochemical parameters of this preparation (subunit composition, content and composition of lipids, heme content, spectra of optical absorption of the oxidized and reduced forms) are determined. Possible presence of two forms of cytochrome b-561 in the chromaffin granules is discussed.
Petrosian
S A
SA
Boiadzhian
A S
AS
Karagezian
K G
KG
D000818:Animals; D002417:Cattle; D002837:Chromaffin Granules / Q000201:enzymology*; D002850:Chromatography, Gel; D003573:Cytochrome b Group / Q000737:chemistry / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006418:Heme / Q000032:analysis; D008055:Lipids / Q000032:analysis; D008970:Molecular Weight
D003573:Cytochrome b Group; D008055:Lipids; C023646:cytochrome b561; D006418:Heme
Ochistka tsitokhroma B-561 iz membran khromaffinnykh granul i ego fizikko-khimicheskie svoĭstva.