Liver ferrochelatase from normal and hexachlorobenzene porphyric rats. Mechanism of drug action.
1. The action of hexachlorobenzene (HCB) on hepatic ferrochelatase was investigated. 2. A direct action of HCB, pentachlorophenol, porphyrins and haem on this enzyme activity was discarded. 3. In HCB porphyric liver there is probably an activator tightly bound to the enzyme. 4. Pyridoxal phosphate (PPL) may be a cofactor of ferrochelatase from both normal and porphyric rats. 5. The PPL would be involved in the binding site of Fe2+ or at least in the approaching of Fe2+ to the active site of the enzyme. 6. The differences found between normal and porphyric preparations could be attributed to conformational changes elicited by the HCB.
Ríos de Molina
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MC
Departamento de Química Biológica, Faculted de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Billi de Catabbi
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S
San Martín de Viale
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LC
D000818:Animals; D001665:Binding Sites; D002850:Chromatography, Gel; D003300:Copper / Q000494:pharmacology; D019327:Copper Sulfate; D005260:Female; D005294:Ferrochelatase / Q000378:metabolism*; D006418:Heme / Q000494:pharmacology; D006581:Hexachlorobenzene / Q000494:pharmacology*; D006358:Hot Temperature; D008099:Liver / Q000201:enzymology*; D010416:Pentachlorophenol / Q000494:pharmacology; D011164:Porphyrias / Q000139:chemically induced / Q000201:enzymology*; D011166:Porphyrins / Q000494:pharmacology; D011487:Protein Conformation / Q000187:drug effects; D011732:Pyridoxal Phosphate / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry
D011166:Porphyrins; D006418:Heme; D006581:Hexachlorobenzene; D011732:Pyridoxal Phosphate; D003300:Copper; D010416:Pentachlorophenol; D005294:Ferrochelatase; D019327:Copper Sulfate