The fan-wise split preparation method for quantitation of peripheral nerve injuries.
The applicability of the fan-wise split preparation method to the analysis of peripheral nerve injuries was explored in an experimental compression injury of the mouse sciatic nerve. The preservation of myelin sheaths was sufficient to enumerate the damaged myelinated fibers. Because of the simplicity and relative ease of the technique, this method could be a useful adjunct for the assessment of therapeutic modalities using experimental animals.
Gomibuchi
S
S
Department of Orthopedic Surgery, Tohoku University School of Medicine, Sendai, Japan.
Iwasaki
Y
Y
Yamamoto
T
T
Miyasaka
Y
Y
Sakurai
M
M
D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009186:Myelin Sheath / Q000648:ultrastructure; D009408:Nerve Compression Syndromes / Q000473:pathology* / Q000503:physiopathology; D009412:Nerve Fibers / Q000648:ultrastructure; D012584:Sciatic Nerve / Q000473:pathology / Q000648:ultrastructure