Comparative analysis of mitochondrial and amyloplast adenylate translocators.
Structurally intact and metabolically competent mitochondria isolated from liquid-culture cells of sycamore (Acer pseudoplatanus L.) were shown to incorporate ADPglucose. Employing the double silicone oil layer filtering centrifugation method, we examined the kinetic properties of the uptake of various adenylates as well as the inhibitory effects exerted by carboxyatractyloside, atractyloside and bongkrekic acid, known specific inhibitors of the mitochondrial adenylate translocator. Immunoblot patterns of peptides derived from the partial proteolytic digestion of the mitochondrial and plastid adenylate translocators were shown to be essentially the same. We conclude that the molecular entities engaged in the adenylate transport system operating in two different organelles, mitochondria and amyloplasts, are very similar.
Pozueta-Romero
J
J
Research Institute for Biochemical Regulation, School of Agriculture, Nagoya University, Japan.
Viale
A M
AM
Akazawa
T
T
D000227:Adenine Nucleotides / Q000378:metabolism*; D000245:Adenosine Diphosphate Glucose / Q000378:metabolism; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology; D001692:Biological Transport; D001865:Bongkrekic Acid / Q000494:pharmacology; D002478:Cells, Cultured; D015151:Immunoblotting; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology*; D000226:Mitochondrial ADP, ATP Translocases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010944:Plants / Q000648:ultrastructure*
D000227:Adenine Nucleotides; D001865:Bongkrekic Acid; D001278:Atractyloside; D000245:Adenosine Diphosphate Glucose; D000226:Mitochondrial ADP, ATP Translocases; C003853:carboxyatractyloside