Clastogenic and aneuploidizing effects of antiblastic busulphan revealed by kinetochore immunofluorescence in CHO cells.
We utilized, in CHO cells, the cytoplasm preservation technique to evaluate the micronucleus frequency at different busulphan concentrations, and the indirect immunofluorescence technique, using sera obtained from patients with scleroderma (CREST variant), to analyze if busulphan-induced micronuclei have kinetochores. Results show that this alkylating agent is capable of causing a significant increase of micronuclei in vitro, a great part (40%) of them having CREST-positive kinetochores. These findings confirm the clastogenic effect of busulphan and reveal a considerable capability of this agent to induce aneuploidy. These results are examined taking into account the high incidence of secondary neoplasias induced by chemotherapy with alkylating agents utilized against primary neoplasias.
Barbata
G
G
Dipartimento di Biologia Cellulare, Università di Palermo, Italy.
Giglio
M C
MC
Granata
G
G
Anzalone
A
A
Carbone
P
P
D000096:Acetone / Q000633:toxicity; D000782:Aneuploidy; D002066:Busulfan / Q000633:toxicity*; D002460:Cell Line; D002503:Centromere / Q000187:drug effects*; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D005455:Fluorescent Antibody Technique; D006801:Humans; D048629:Micronuclei, Chromosome-Defective / Q000648:ultrastructure; D015162:Micronucleus Tests; D012595:Scleroderma, Systemic / Q000097:blood
D000096:Acetone; D002066:Busulfan