Mononuclear cell (MNC) concentration from the marrow harvest by automatic system.
The availability of reliable and standardizable procedures with low contamination risk and less prominent loss in MNC, would solve the existing perplexities about the systematic use of this passage. We tried to experiment Areman's et al protocol that utilizes the CS 3000 blood cell processor without gradient, making some changes and comparing the results with those achieved with the COBE 2991 on Ficoll. Some aspects should be defined, but the preliminary results are undoubtedly of great interest.
Franchini
E
E
Blood Bank, G. Gaslini Research Children's Hospital, Genoa, Italy.
Porlezza
M
M
D001331:Automation / Q000295:instrumentation; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000379:methods*; D002469:Cell Separation / Q000295:instrumentation*; D005069:Evaluation Studies as Topic; D005362:Ficoll; D006801:Humans; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000637:transplantation
D005362:Ficoll