title: Cyclic AMP-receptor protein activity in rat preputial cells.
abstract: Cells from the rat preputial gland--a type of sebaceous gland--exhibited specific responsiveness of cyclic 3',5'-adenosine monophosphate (AMP) dependent protein kinase to stimulation by agents that elevate intracellular cyclic AMP. Electron microscopy shows that the rat preputial gland resembles the human sebaceous gland, not only in terms of containing a sebocyte-like population of cells in an acinar arrangement at different maturational stages, but also in the morphology of its organelles such as abundant and sometimes atypical mitochondria, many perinuclear lysosomes with crystalline inclusions, lipid droplets of various sizes, and peroxisomes. Other cell types, among them duct and inflammatory cells, were evident in the tissue sections, but constituted a minor component. Responses to stimulation of the adenylate cyclase-protein kinase pathways were determined using preputial cells that had been both freshly dispersed and grown in monolayer culture. Stimulation with isoproterenol (IPR) or forskolin (FS) resulted in both cases in an increase of cyclic AMP binding of the regulatory (R) subunits of cyclic AMP-dependent protein kinase, as determined by photoaffinity labeling of R subunits with an azido analog of cyclic AMP ([32P]-8-azido cyclic AMP). Cells from the epidermis under comparable conditions responded to a lesser degree and with a different distribution of R subunit isoforms. There are, therefore, differences in receptor activity as well as in the transduction pathways between the two types of epithelial cell populations. These results indicate that the preputial gland contains precursor cells that differentiate in culture to retain specific molecular mechanisms of action mediated via cyclic AMP.
authors.affiliation: University of Chicago, Pritzker School of Medicine, Illinois.
authors.forename: M I
authors.initials: MI
authors.lastname: Mednieks
authors.forename: S J
authors.initials: SJ
authors.lastname: Laurent
authors.forename: A R
authors.initials: AR
authors.lastname: Hand
authors.forename: R L
authors.initials: RL
authors.lastname: Rosenfield
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D008297:Male; D008858:Microscopy, Phase-Contrast; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D011953:Receptors, Cyclic AMP / Q000378:metabolism*; D012627:Sebaceous Glands / Q000166:cytology / Q000648:ultrastructure*
chemical_list: D011953:Receptors, Cyclic AMP