Nonmammalian vertebrate skeletal muscles express two triad junctional foot protein isoforms.
Mammalian skeletal muscles express a single triad junctional foot protein, whereas avian muscles have two isoforms of this protein. We investigated whether either case is representative of muscles from other vertebrate classes. We identified two foot proteins in bullfrog and toadfish muscles on the basis of (a) copurification with [3H]epiryanodine binding; (b) similarity to avian muscle foot proteins in native and subunit molecular weights; (c) recognition by anti-foot protein antibodies. The bullfrog and toadfish proteins exist as homooligomers. The subunits of the bullfrog muscle foot protein isoforms are shown to be unique by peptide mapping. In addition, immunocytochemical localization established that the bullfrog muscle isoforms coexist in the same muscle cells. The isoforms in either bullfrog and chicken muscles have comparable [3H]epiryanodine binding capacities, whereas in toadfish muscle the isoforms differ in their levels of ligand binding. Additionally, chicken thigh and breast muscles differ in the relative amounts of the two isoforms they contain, the amounts being similar in breast muscle and markedly different in thigh muscle. In conclusion, in contrast to mammalian skeletal muscle, two foot protein isoforms are present in amphibian, avian, and piscine skeletal muscles. This may represent a general difference in the architecture and/or a functional specialization of the triad junction in mammalian and nonmammalian vertebrate muscles.
Olivares
E B
EB
Department of Pharmacology, University of Nevada, Reno 89557.
Tanksley
S J
SJ
Airey
J A
JA
Beck
C F
CF
Ouyang
Y
Y
Deerinck
T J
TJ
Ellisman
M H
MH
Sutko
J L
JL
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002645:Chickens; D005399:Fishes; D008970:Molecular Weight; D009124:Muscle Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D011487:Protein Conformation; D011817:Rabbits; D011892:Rana catesbeiana; D011950:Receptors, Cholinergic / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D019837:Ryanodine Receptor Calcium Release Channel
D009124:Muscle Proteins; D011950:Receptors, Cholinergic; D019837:Ryanodine Receptor Calcium Release Channel