Cross-linking a maturation-dependent ram sperm plasma membrane antigen induces the acrosome reaction.
ESA152 is a highly hydrophobic 18 kDa sialoglycoprotein, which becomes expressed on ram sperm in the proximal cauda epididymis. ESA 152 is expressed on all regions of the sperm surface, most strongly on the posterior region of the head, most weakly on the anterior region of the head. In this paper, we show that induction of the acrosome reaction with Ca2+ ionophore causes ESA152 to be redistributed from the posterior to the anterior region of the head plasma membrane. Cross-linking ESA152 with bivalent antibody causes similar redistribution and induces the acrosome reaction. Induction of the acrosome reaction with ESA152 antibody requires Ca2+ but is insensitive to (10 ng/ml) pertussis toxin.
McKinnon
C A
CA
Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.
Weaver
F E
FE
Yoder
J A
JA
Fairbanks
G
G
Wolf
D E
DE
D000177:Acrosome / Q000502:physiology*; D000818:Animals; D000937:Antigen-Antibody Reactions; D001427:Bacterial Toxins / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium; D002462:Cell Membrane / Q000276:immunology; D007152:Immunologic Capping; D008297:Male; D008854:Microscopy, Electron; D037342:Pertussis Toxin; D052899:Pore Forming Cytotoxic Proteins; D012795:Sialoglycoproteins / Q000276:immunology / Q000502:physiology*; D013077:Sperm Head / Q000276:immunology* / Q000502:physiology; D013094:Spermatozoa; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D001427:Bacterial Toxins; D052899:Pore Forming Cytotoxic Proteins; D012795:Sialoglycoproteins; D010566:Virulence Factors, Bordetella; D000001:Calcimycin; C032676:aerolysin; D037342:Pertussis Toxin; D002118:Calcium