title: Serum profiles of steroid hormones in patients with Cushing's syndrome determined by a new HPLC/RIA method.
abstract: We developed a method for simultaneously measuring steroid hormones in very small volumes of serum, using a combination of high-performance liquid chromatography (HPLC) and radioimmunoassay (RIA). By this method, aldosterone, cortisol, 11-deoxycortisol, estrone, estradiol, androstenedione, dehydroepiandrosterone, deoxycorticosterone, 17-hydroxyprogesterone, testosterone, pregnenolone, and progesterone could be determined in a single 100-microL aliquot of serum from normal adults and patients with Cushing's syndrome. The steroid profile associated with Cushing's syndrome caused by adrenal adenoma was quite distinct from that associated with the syndrome caused by adrenal hyperplasia. Serum concentrations of androstenedione, dehydroepiandrosterone, estrone, estradiol, 17-hydroxyprogesterone, pregnenolone, and testosterone were significantly higher in patients with adrenal hyperplasia than in those with an adenoma. We compared the results of this HPLC/RIA method with those of 125I RIAs. The use of a HPLC/RIA system to obtain an accurate and sensitive profile of a range of serum steroids, as described here, obviates the need for large volumes of blood.
authors.affiliation: First Department of Internal Medicine, Toho University School of Medicine, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ueshiba
authors.forename: M
authors.initials: M
authors.lastname: Segawa
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Miyachi
authors.forename: M
authors.initials: M
authors.lastname: Irie
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003429:Cross Reactions; D003480:Cushing Syndrome / Q000097:blood*; D005260:Female; D006728:Hormones / Q000097:blood*; D006801:Humans; D008297:Male; D011863:Radioimmunoassay / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D013256:Steroids / Q000097:blood*
chemical_list: D006728:Hormones; D013256:Steroids