title: Determination of lectin-sugar binding constants by microequilibrium dialysis coupled with high performance liquid chromatography.
abstract: We used high performance liquid chromatography to determine the concentrations of free ligands after equilibrium dialysis to examine lectin-sugar interactions. The binding of p-nitrophenyl 1-thio-alpha-mannoside, p-nitrophenyl N-acetyl-1-thio-beta-glucosaminide, and the pyridylamino derivative of Man6GlcNAc2 to concanavalin A and Triticum vulgaris lectin was examined. The binding constant, Ka, and the concentration of total binding sites, [L]t, were calculated from the trace amounts of sugars and lectins using the equation, [S]/[LS] = 1/Ka[L]t + [S]/[L]t, where [S] is the concentration of free ligand and [LS] the concentration of bound ligand.
authors.affiliation: Department of Chemistry, Osaka University College of Science.
authors.forename: T
authors.initials: T
authors.lastname: Mega
authors.forename: S
authors.initials: S
authors.lastname: Hase
mesh_terms: D002241:Carbohydrates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003956:Dialysis / Q000295:instrumentation / Q000379:methods; D007700:Kinetics; D037102:Lectins / Q000737:chemistry*; D008024:Ligands; D008433:Mathematics; D008962:Models, Theoretical; D011485:Protein Binding; D013997:Time Factors
chemical_list: D002241:Carbohydrates; D037102:Lectins; D008024:Ligands