Convergence of Ets- and notch-related structural motifs in a heteromeric DNA binding complex.
Analysis of the heteromeric DNA binding protein GABP has revealed the interaction of two distinct peptide sequence motifs normally associated with proteins located in different cellular compartments. The alpha subunit of GABP contains an 85-amino acid segment related to the Ets family of DNA binding proteins. The ETS domain of GABP alpha facilitates weak binding to DNA and, together with an adjacent segment of 37 amino acids, mediates stable interaction with GABP beta. The beta subunit of GABP contains four imperfect repeats of a sequence present in several transmembrane proteins including the product of the Notch gene of Drosophila melanogaster. These amino-terminal repeats of GABP beta mediate stable interaction with GABP alpha and, when complexed with GABP alpha, directly contact DNA. These observations provide evidence for a distinct biochemical role for the 33-amino acid repeats, and suggest that they may serve as a module for the generation of specific dimerization interfaces.
Thompson
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Howard Hughes Research Laboratories, Carnegie Institution of Washington, Department of Embryology, Baltimore, MD 21210.
Brown
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McKnight
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D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003432:Cross-Linking Reagents; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D051268:GA-Binding Protein Transcription Factor; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry; D011518:Proto-Oncogene Proteins / Q000737:chemistry; D050783:Proto-Oncogene Proteins c-ets; D051381:Rats; D011994:Recombinant Proteins; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism
D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D051268:GA-Binding Protein Transcription Factor; C495649:Gabpa protein, rat; C495653:Gabpb2 protein, rat; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D009841:Oligonucleotides; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D011994:Recombinant Proteins; D014157:Transcription Factors; D004247:DNA