[Rhythmic changes in protein synthesis in mouse pancreatocytes after food stimulation, in alloxan diabetes and in isoproterenol exposure].
Radioautography revealed data on protein synthesis dynamics in pancreacytes of intact mice, subjected to alloxan diabetes and isoproterenol (ISP) injection after synchronized secretion. Rhythmic fluctuation of protein synthesis with maximum uptake of 3H-leucine 40 and 80 min after treatment was observed in intact animals. The above maximums correlated with the increase in the cell size. In alloxan diabetes, injections of ISP and their combination the rhythm of protein synthesis is retained, the total intensity of isotope uptake increases significantly, but at the same time secretion periods, particularly in the latter case decrease. The results obtained revealed the existence of endogenic rhythms in protein synthesis with certain secretion plasticity within pancreacytes.
Khamdan
R
R
Shubnikova
E A
EA
Pogodina
L S
LS
D000818:Animals; D001345:Autoradiography; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005247:Feeding Behavior / Q000187:drug effects / Q000502:physiology*; D007545:Isoproterenol / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010179:Pancreas / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010507:Periodicity; D014176:Protein Biosynthesis; D011506:Proteins / Q000187:drug effects; D014316:Tritium
D011506:Proteins; D014316:Tritium; D007930:Leucine; D007545:Isoproterenol
Ritmicheskie izmeneniia sinteza belka v pankreatsitakh myshi posle pishchevoĭ stimuliatsii, pri alloksanovom diabete i pri vozdeĭstvii izoproterenolom.