Differential solubility behaviour in poly(ethylene glycol) solutions of glucose 6-phosphate and 6-phosphogluconate dehydrogenases from bone marrow, reticulocytes and erythrocytes.
Precipitation of glucose 6-phosphate dehydrogenase by poly(ethylene glycol) depends on both pH and the source of haemolysate. An increase in pH from 5 to 7 leads to an increase in the polymer concentration required for precipitation. At any pH, polymer concentration needed to precipitate the enzyme increases in the order bone marrow less than reticulocytes less than erythrocytes. This differential behaviour seems to be due to variations on the effect of pH on the state of aggregation and/or differences in the intrinsic solubility of the enzyme present in the three haemolysates. In contrast, precipitation profile of 6-phosphogluconate dehydrogenase only slightly moves towards higher concentrations of PEG when raising the pH from 5 to 7, being similar at each single pH in the three haemolysates.
Delgado
C
C
Departamento de Bioquimica y Biologia Molecular, Universidad de Alcala, Madrid, Spain.
Tejedor
M C
MC
Luque
J
J
D000818:Animals; D001853:Bone Marrow / Q000201:enzymology*; D011232:Chemical Precipitation; D004912:Erythrocytes / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D010734:Phosphogluconate Dehydrogenase / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D051381:Rats; D011919:Rats, Inbred Strains; D012156:Reticulocytes / Q000201:enzymology; D012995:Solubility
D011092:Polyethylene Glycols; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase