Altered intracellular calcium regulation in human colorectal cancers and in "normal" adjacent mucosa.
Intracellular calcium [(Ca2+)i] was measured in human colonic epithelia derived from control mucosa from noncancer patients, from grossly "normal" mucosa taken 10-30 cm proximal to primary colorectal cancers, and from colorectal cancers. (Ca2+)i was measured first in cells bathed by calcium-free solution and again after adding 1 mM calcium. Compared to control mucosa, (Ca2+)i was 27% lower in cancer cells in the presence of 1 mM extracellular calcium, whereas (Ca2+)i was elevated more than 3-fold in the adjacent "normal" mucosa from cancer patients in both media. These results suggest altered (Ca2+)i levels in malignant cells and the presence of a "field defect" in (Ca2+)i regulation in "normal" colonic mucosa adjacent to colorectal cancers.
Edelstein
P S
PS
University of California, San Diego 92103.
Thompson
S M
SM
Davies
R J
RJ
D002118:Calcium / Q000378:metabolism*; D003106:Colon; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D005456:Fluorescent Dyes; D016257:Fura-2 / Q000031:analogs & derivatives; D006706:Homeostasis; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D012004:Rectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; C049925:fura-2-am; D002118:Calcium; D016257:Fura-2