Oligonucleotide purification in milligram quantities.
An inexpensive and simple procedure for the purification of synthetic oligonucleotides is described. Hands-on time is only 30 to 40 minutes and multiple samples can be prepared simultaneously. One- to two-milligram quantities can easily be handled by a single column with no further purification required for the DNA to be used in a wide variety of molecular biological uses.
Freie
B
B
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202.
Larsen
S H
SH
D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000302:isolation & purification*; D013997:Time Factors
D009841:Oligonucleotides