title: DNA ploidy of primary breast cancer and local recurrence after breast-conserving therapy.
abstract: The value of DNA-flow cytometry and clinico-pathological prognostic factors for the prediction of local recurrences after breast-conserving therapy (BCT) were evaluated in a retrospective study. Thirty-one patients with a local recurrence were compared with 31 matched patients without a local recurrence. Morphology and DNA-indices of the local recurrences and their corresponding primary tumours were compared. Ductal carcinoma in situ was present significantly more often in the group with a primary recurring tumour, than in the matched group (P less than 0.001), and the same holds for lobular carcinoma (n = 5). Half of the tumours that recurred had macroscopically positive surgical margins compared to about one-fourth of the matched group. Fifty-six per cent of the DNA-aneuploid stemlines in cases with local recurrence were present in the corresponding primary tumour as well (confidence limits 45%-75%), an indication that the majority of local recurrences are true recurrences and not independently developed tumours. The lack of similarity of DNA stemlines between some primary DNA-aneuploid tumours and their local recurrences indicates that these tumours had developed independently. The percentage of DNA-aneuploid cases in the group with local recurrence (89%) did not differ significantly from that in the matched group (70%). However, the findings suggest a selective recurrence of DNA-diploid stemlines. This might indicate increased resistance of DNA-diploid tumour cells to radiotherapy as compared with the resistance level in DNA-aneuploid cells.
authors.affiliation: Department of Pathology, Faculty of Medicine, Leiden University Hospital, University of Leiden, The Netherlands.
authors.forename: H
authors.initials: H
authors.lastname: Beerman
authors.forename: B A
authors.initials: BA
authors.lastname: Bonsing
authors.forename: M J
authors.initials: MJ
authors.lastname: van de Vijver
authors.forename: J
authors.initials: J
authors.lastname: Hermans
authors.forename: P M
authors.initials: PM
authors.lastname: Kluin
authors.forename: R J
authors.initials: RJ
authors.lastname: Caspers
authors.forename: C J
authors.initials: CJ
authors.lastname: van de Velde
authors.forename: C J
authors.initials: CJ
authors.lastname: Cornelisse
mesh_terms: D000782:Aneuploidy; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology* / Q000532:radiotherapy / Q000601:surgery; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D005500:Follow-Up Studies; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D011003:Ploidies; D011879:Radiotherapy Dosage; D012189:Retrospective Studies
chemical_list: D004273:DNA, Neoplasm