Nuclear transplantation in bovine embryo: fine structural and autoradiographic studies.
Nucleolar fine structure, "blebbing" activity of nuclear envelope, and activation of heterogeneous nuclear RNA (hnRNA) synthesis were studied in bovine reconstructed embryos obtained by electrofusion of a single eight-cell blastomere with an enucleated oocyte. Developmental progress of nucleolar fine structure and hnRNA synthesis are arrested during three cell cycles following fusion. The activation of both appears during the eight-cell stage of the reconstructed embryo, after the same number of cell cycles after fusion as in nonmanipulated bovine embryos after fertilization. "Blebbing" activity of nuclear envelope, which is already absent in original blastomeres, reappears after fusion and continues for the next two cell cycles. From the present results, it can be concluded that the donor nuclei are arrested after fusion in morphology and function. Their reactivation corresponds to the developmental pattern typical for normal bovine embryos.
Kanka
J
J
Department of Genetics, Czechoslovak Academy of Sciences, Libĕchov.
Fulka
J
J
Fulka
J
J
Petr
J
J
D000818:Animals; D001345:Autoradiography; D001755:Blastocyst / Q000378:metabolism / Q000648:ultrastructure; D002417:Cattle; D002459:Cell Fusion / Q000502:physiology; D002466:Cell Nucleolus / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000648:ultrastructure*; D002970:Cleavage Stage, Ovum / Q000378:metabolism / Q000648:ultrastructure; D004622:Embryo, Mammalian / Q000378:metabolism / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009685:Nuclear Envelope / Q000648:ultrastructure*; D053652:Nuclear Transfer Techniques; D014158:Transcription, Genetic