A method for mini-immunoblots that allows screening of large numbers of samples.
A method that allows the use of immunoblots as a screening test for large numbers of samples is described. Preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis is run to a separation distance of 2 cm and the samples are blotted onto nitrocellulose. One-millimeter-wide strips are cut and immobilized on double-sided tape. Adjacent strips are separated by plastic rods. After incubation with the first antibody, whole slides are processed together as in histology. The method has been successfully applied to the primary screening of hybridoma supernatants and is generally applicable in situations where large numbers of samples must be tested in a short time.
Kapfhammer
J P
JP
Brain Research Institute, University of Zürich, Switzerland.
D000818:Animals; D003101:Collodion; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas; D015151:Immunoblotting / Q000379:methods*; D009185:Myelin Proteins / Q000032:analysis; D051381:Rats
D003470:Culture Media; D009185:Myelin Proteins; D003101:Collodion