High-performance liquid chromatographic analysis of free palmitic and stearic acids in cerebrospinal fluid.
A relatively simple method for extraction of free fatty acids from cerebrospinal fluid with aminopropyl bonded-phase columns, and the estimation of palmitic acid (C16:0) and stearic acid (C18:0) concentrations by high-performance liquid chromatographic analysis is described. The values of C16:0 and C18:0 in patients with non-neurological disorders lie within a narrow range, with a mean (+/- S.D.) of 4.02 +/- 0.33 micrograms/ml for C16:0 and 2.72 +/- 0.39 micrograms/ml for C18:0.
Zamir
I
I
Department of Inorganic and Analytical Chemistry, Hebrew University, Jerusalem, Israel.
Grushka
E
E
Cividalli
G
G
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D010169:Palmitic Acids / Q000134:cerebrospinal fluid*; D013229:Stearic Acids / Q000134:cerebrospinal fluid*
D010169:Palmitic Acids; D013229:Stearic Acids