Sequence of 18S rDNA of actinorhizal Alnus glutinosa (Betulaceae).
The small subunit ribosomal DNA for a woody actinorhizal, Alnus glutinosa, was isolated by the PCR method. Amplification products were cloned into the Bluescript SK- vector. Full sequence, 1698 bp, was obtained with NS1 to NS8 primers. Sequence alignments were made by UWGCG sequence data analysis computer programs. 18S rDNA sequence of A. glutinosa was compared to analogous segments of four other angiosperms, tomato, rice, maize and soybean. Sequence homologies are discussed and application for the technique is suggested.
Savard
L
L
Département des Sciences Forestières, Faculté de Foresterie et de Géomatique, Université Laval, Québec, Canada.
Lalonde
M
M
D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012984:Software
D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S