Rifampin-resistant replication of pBR322 derivatives in Escherichia coli cells induced for the SOS response.
Replication of plasmid pBR322 in Escherichia coli cells normally requires RNA synthesis and thus is sensitive to rifampin, an inhibitor of RNA polymerase. In cells induced for the SOS response, however, derivatives of pBR322 were found to replicate in the presence of rifampin. This rifampin-resistant replication of pBR322 requires the insertion of certain sequences of DNA. The replication depends on recF+ and DNA polymerase I.
Magee
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TR
Department of Biology, University of New Mexico, Albuquerque 87131.
Kogoma
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T
D002876:Chromosomes, Bacterial; D004256:DNA Polymerase I / Q000378:metabolism; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D010957:Plasmids / Q000187:drug effects*; D012093:Replicon; D012293:Rifampin / Q000494:pharmacology*; D013014:SOS Response, Genetics / Q000187:drug effects*; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial; D004256:DNA Polymerase I; D012293:Rifampin