title: A sandwich enzyme-linked immunosorbent assay for beta 2-glycoprotein I.
abstract: A solid-phase sandwich enzyme-linked immunosorbent assay for determining beta 2-glycoprotein I in urine has been developed. It has a working concentration range of 5-40 micrograms/L and a detection limit of approximately 1.4 micrograms/L. The within-plate coefficient of variation (CV) falls between 1.4% and 2.1%, and the between-batch CV ranges from 5.2 to 6.0%. Recovery of beta 2-glycoprotein I added to urine varies between 96 and 110%. The assay can also be used for determining beta 2-glycoprotein I in serum.
authors.affiliation: Department of Chemical Pathology, University College and Middlesex School of Medicine, London, UK.
authors.forename: P A
authors.initials: PA
authors.lastname: Sansom
authors.forename: C T
authors.initials: CT
authors.lastname: Marlow
authors.forename: M
authors.initials: M
authors.lastname: Lapsley
authors.forename: F V
authors.initials: FV
authors.lastname: Flynn
mesh_terms: D000328:Adult; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006023:Glycoproteins / Q000652:urine*; D006801:Humans; D007674:Kidney Diseases / Q000175:diagnosis; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053482:beta 2-Glycoprotein I
chemical_list: D006023:Glycoproteins; D053482:beta 2-Glycoprotein I