Changes in fibronectin mRNA splicing with in vitro passage.
Senescent human fibroblasts produce larger fibronectin molecules with altered binding properties. To determine if this change could involve alternative splicing of fibronectin precursor mRNA, we developed an approach using reverse transcription and the polymerase chain reaction to study fibronectin mRNA splicing at each of the three alternatively spliced regions. Two of the three regions showed changes with in vitro passage incorporation of the ED-A region increased 8 fold.
Burke
E M
EM
Laboratory of Molecular Genetics, National Institute on Aging, Baltimore, MD 21224.
Danner
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DB
D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005353:Fibronectins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*
D005353:Fibronectins; D012333:RNA, Messenger; D004247:DNA