Simplified sample preparation for fluoride determination in biological material.
A simple and rapid preparation method for the determination of fluoride in biological materials (blood and food) of various origins, is described. A homogenized sample was placed in a plastic diffusion cell and calcium phosphate added, it was then dried at 55 degrees C and treated with 70% HClO4 and 40% AgClO4. After digestion for 24 h at 55 degrees C, the fluorides released were fixed on the upper part of a diffusion cell containing a thin layer of NaOH. The analyses of the diffused fluoride were carried out with an ion-selective electrode. The proposed microdiffusion method, without mineralization, enables quantitative separation of the fluoride from the biological samples.
Nedeljković
M
M
Department of Toxicological Chemistry, School of Pharmacy, University of Belgrade, Yugoslavia.
Antonijević
B
B
Matović
V
V
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002417:Cattle; D004566:Electrodes; D005459:Fluorides / Q000032:analysis* / Q000097:blood; D005504:Food Analysis; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008892:Milk / Q000737:chemistry
D007202:Indicators and Reagents; D005459:Fluorides