Plasma membrane properties regulating the sensitivity of leukemia, lymphoma, and solid tumor cells to merocyanine 540-sensitized photoirradiation.
Merocyanine 540 (MC 540) is a photosensitizing dye that has been used in a phase I clinical trial for the purging of leukemia and lymphoma cells from autologous bone marrow grafts. In this paper we examine the role of plasma membrane negative charge, plasma membrane fluidity, and plasma membrane hydrophobicity in the regulation of a cell's susceptibility to MC 540-sensitized photoirradiation. Among solid tumor cells, we found an inverse correlation between surface electronegativity, affinity for dye molecules, and susceptibility to MC 540-sensitized photoinactivation. That is, the least electronegative cells bound the highest amount of dye and were the most susceptible to dye-sensitized photoirradiation. By contrast, no such correlations were found among leukemia/lymphoma cells. This suggested that dye binding and susceptibility to MC 540-mediated photodynamic damages are regulated differently in hematopoietic/lymphopoietic and solid tumor cells.
Smith
O M
OM
Department of Pediatrics, Medical College of Wisconsin, Milwaukee 53226.
Gaffney
D K
DK
Anderson
M S
MS
McOlash
L
L
Schober
S L
SL
Sieber
F
F
D001853:Bone Marrow / Q000187:drug effects* / Q000528:radiation effects; D001854:Bone Marrow Cells; D002462:Cell Membrane / Q000187:drug effects*; D002469:Cell Separation; D006801:Humans; D007938:Leukemia / Q000188:drug therapy*; D008027:Light; D008223:Lymphoma / Q000188:drug therapy*; D008560:Membrane Fluidity; D009369:Neoplasms / Q000188:drug therapy*; D009439:Neuraminidase / Q000494:pharmacology; D011744:Pyrimidinones / Q000633:toxicity*; D011838:Radiation-Sensitizing Agents; D012995:Solubility; D013499:Surface Properties; D014357:Trypsin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D011744:Pyrimidinones; D011838:Radiation-Sensitizing Agents; C003954:merocyanine dye; D009439:Neuraminidase; D014357:Trypsin