A rapid and simple method for the preparation of a monospecific antibody to ovine haptoglobin.
Antisera specific to ovine haptoglobin were obtained by the immunization of rabbits with their own insolubilized haemoglobin after it had been allowed to react with a pool of haptoglobin-rich ovine serum. This method exploits the unique property of haptoglobin to bind with great specificity and affinity to both homologous and heterologous haemoglobin. The procedure is rapid, simple, inexpensive and could be readily applied to the production of antisera against the haptoglobins of other species.
Levieux
D
D
I.N.R.A., Station de Recherches sur la Viande, Centre de Recherches de Clermont-Fd-Theix, Saint-Genès-Champanelle, France.
Venien
A
A
D000327:Adsorption; D000818:Animals; D000918:Antibody Specificity; D006242:Haptoglobins / Q000276:immunology*; D006454:Hemoglobins / Q000276:immunology; D007106:Immune Sera / Q000276:immunology*; D007114:Immunization; D008863:Microspheres; D011817:Rabbits; D012756:Sheep; D012995:Solubility
D006242:Haptoglobins; D006454:Hemoglobins; D007106:Immune Sera