title: Modulation of elastase-like activity in fibroblasts stimulated with elastin peptides.
abstract: Elastin-derived peptides, kappa-elastin, prepared by chemical degradation of insoluble elastin from bovine ligamentum nuchae, were shown to increase the elastase-like activity in the culture medium and cell fractions in fibroblasts. Preincubation of cells with nifedipine (calcium channel blocker) and trifluoperazine (calmodulin antagonist) induced a decrease in the activities of the enzyme under study. These data suggest the possibility of pharmacological modulation of the biological effects induced by elastin-derived peptides.
authors.affiliation: Department of Biochemistry and Chemistry, Silesian Medical Academy, Katowice, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Gmiński
authors.forename: L
authors.initials: L
authors.lastname: Weglarz
authors.forename: M
authors.initials: M
authors.lastname: Drózdz
authors.forename: P
authors.initials: P
authors.lastname: Sułkowski
authors.forename: M
authors.initials: M
authors.lastname: Goss
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004549:Elastin / Q000302:isolation & purification / Q000494:pharmacology*; D005347:Fibroblasts / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology; D009543:Nifedipine / Q000494:pharmacology; D010196:Pancreatic Elastase / Q000378:metabolism*; D010446:Peptide Fragments / Q000302:isolation & purification / Q000494:pharmacology*; D014268:Trifluoperazine / Q000494:pharmacology
chemical_list: D010446:Peptide Fragments; D014268:Trifluoperazine; D004549:Elastin; D010196:Pancreatic Elastase; D009543:Nifedipine