Vitamin C analysis in whole blood, plasma and cells using reduced glutathione as preservative (stabilizer): losses and redistribution.
The losses and redistribution of vitamin C in blood fractions (stabilized with reduced glutathione) during 4 hours of storage at room temperature in the dark was studied among 27 elderly women. Storage did not have a statistically significant (p greater than 0.05) effect on the mean vitamin C levels in cells, plasma and whole blood. The results point to a redistribution of vitamin C among blood fractions. It is concluded that vitamin C concentrations in cells rather than in plasma should be the principal indicator of the vitamin C status when blood (stabilized with reduced glutathione) is stored at room temperature before specimen handling.
Löwik
M R
MR
TNO-CIVO Toxicology and Nutrition Institute, Zeist, The Netherlands.
Schrijver
J
J
Wedel
M
M
D000368:Aged; D000369:Aged, 80 and over; D001205:Ascorbic Acid / Q000097:blood*; D001773:Blood Cells / Q000737:chemistry*; D001793:Blood Preservation / Q000379:methods; D005260:Female; D005978:Glutathione; D006801:Humans; D009752:Nutritional Status; D010949:Plasma / Q000737:chemistry*; D013696:Temperature
D005978:Glutathione; D001205:Ascorbic Acid