Rapid screening for cell surface binding monoclonal antibodies.
A procedure is described which allows for rapid detection of cell surface binding or cytoskeleton binding monoclonal antibodies. At the same time this procedure ensures that selected antibodies will be useful in Western blot analysis. This procedure including the cytochemistry and Western blot analysis requires only 100 microliters of supernatant and can be done directly from the original 96 well plates into which the fusion was plated. One person can easily assay several hundred supernatants in one day for the ability to stain both cells and selected proteins in Western blot analysis.
Wheelock
M J
MJ
University of Toledo, Ohio 43606.
D000469:Alkaline Phosphatase / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000096:biosynthesis; D000954:Antigens, Surface / Q000276:immunology*; D015153:Blotting, Western; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D013499:Surface Properties
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000469:Alkaline Phosphatase