Process monitoring by parallel column gradient elution chromatography.
A system was developed that generates two gradients simultaneously, 180 degrees out of phase. Essential elements of the system were a pump delivering solvent A at a constant velocity, a pump delivering solvent B at a constant velocity, two mixing chambers, and a ten-port valve. By use of this gradient device and two reversed-phase columns packed with 1000-A pore diameter, 8-microns particle size macroporous poly-(styrene-divinylbenzene) resin, six proteins were separated in 40 s in a single gradient cycle.
Thévenon-Emeric
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Department of Chemistry, Purdue University, West Lafayette, Indiana 47907.
Regnier
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D002845:Chromatography / Q000295:instrumentation*; D007202:Indicators and Reagents; D011506:Proteins / Q000032:analysis
D007202:Indicators and Reagents; D011506:Proteins