GDP beta S antagonizes whole-cell current responses to excitatory amino acids.
Guanine nucleotides have been predicted to be NMDA antagonists in tissue binding studies. Using the patch-clamp technique, we now show that the guanine nucleotide GDP beta S produces a rapidly reversible antagonism of NMDA, kainate, and, less potently, quisqualate whole-cell current responses. Furthermore, it does not appear that this antagonism is effected intracellularly. Our results suggest a novel extracellular role for guanine nucleotides apart from their traditional intracellular actions on G-proteins.
Budson
A E
AE
Department of Neurology, Children's Hospital, Boston, MA 02115.
Jackson
P S
PS
Lipton
S A
SA
D000818:Animals; D006153:Guanosine Diphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D066298:In Vitro Techniques; D007608:Kainic Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D016202:N-Methylaspartate / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D016318:Quisqualic Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051381:Rats; D012165:Retinal Ganglion Cells / Q000187:drug effects / Q000502:physiology*; D013477:Superior Colliculi / Q000502:physiology*; D013873:Thionucleotides / Q000494:pharmacology*
D013873:Thionucleotides; D006153:Guanosine Diphosphate; D016202:N-Methylaspartate; C023427:guanosine 5'-O-(2-thiodiphosphate); D016318:Quisqualic Acid; D007608:Kainic Acid