title: Protein structure and cDNA nucleotide sequence of the Japanese quail alpha A globin.
abstract: The primary structure of the alpha polypeptide chain (alpha A) of the major component (QII) of Japanese quail hemoglobin was determined by protein and cDNA sequence analysis. The amino-acid sequences of all the soluble tryptic peptides were determined by the conventional protein sequencing technology. The sequence of the remaining portion, which contained an insoluble "core region", was determined through determination of the cDNA nucleotide sequence. The cDNA clones coding for the alpha A globin were isolated from the quail reticulocyte cDNA library, mapped by restriction enzyme digestion, and the nucleotide sequence was determined completely. The primary structure of quail alpha A globin shows a close similarity to that of chicken alpha A globin.
authors.affiliation: Department of Biochemistry, University of the Ryukyus Faculty of Medicine, Okinawa.
authors.forename: Y
authors.initials: Y
authors.lastname: Eguchi
authors.forename: H
authors.initials: H
authors.lastname: Takei
authors.forename: M
authors.initials: M
authors.lastname: Oshiro
authors.forename: T
authors.initials: T
authors.lastname: Toda
authors.forename: Y
authors.initials: Y
authors.lastname: Nakashima
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003370:Coturnix; D004247:DNA / Q000737:chemistry*; D015698:Genomic Library; D005914:Globins / Q000737:chemistry / Q000235:genetics*; D006454:Hemoglobins / Q000235:genetics*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics*; D012156:Reticulocytes / Q000737:chemistry
chemical_list: D006454:Hemoglobins; D010446:Peptide Fragments; C070077:alpha(A) globin; D005914:Globins; D004247:DNA