Primary structures of the bombesin-like neuropeptides in frog brain show that bombesin is not the amphibian gastrin-releasing peptide.
Peptides displaying gastrin-releasing peptide/bombesin-like immunoreactivity were isolated in pure form from an extract of the brain of the European green frog, Rana ridibunda. The primary structure of the more abundant peptide was established as: Gly-Ser-His-Trp-Ala-Val-Gly-His-Leu-Met. NH2. This sequence shows one substitution (Ser for Asn) compared with mammalian gastrin-releasing peptide (18-27) (neuromedin C). The extract also contained gastrin-releasing peptide but bombesin was absent. The data indicate that bombesin is not the amphibian counterpart of gastrin-releasing peptide.
Conlon
J M
JM
Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178.
O'Harte
F
F
Vaudry
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001839:Bombesin / Q000737:chemistry*; D001923:Brain Chemistry; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D019886:Gastrin-Releasing Peptide; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry* / Q000302:isolation & purification; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D011895:Rana ridibunda; D012689:Sequence Homology, Nucleic Acid; D013056:Spectrophotometry, Ultraviolet; D013552:Swine
D009479:Neuropeptides; D010455:Peptides; D019886:Gastrin-Releasing Peptide; D001839:Bombesin