Protein B: a versatile bacterial Fc-binding protein selective for human IgA.
Protein B, a selective bacterial IgA Fc-binding protein isolated from group B streptococci, has been used to quantify fluid phase and immobilized human IgA. Protein B detects both human IgA1 and IgA2 subclasses and is also reactive with secretory IgA. Protein B can be used immobilized to microtiter plates to capture IgA or following biotinylation as a tracer for fluid phase or immobilized human IgA. The studies presented here suggest protein B will prove to be a valuable reagent for quantitative immunochemical procedures involving human IgA antibodies and facilitate a variety of studies of IgA responses in man.
Faulmann
E L
EL
Department of Microbiology, Medical College of Ohio, Toledo 43699-0008.
Duvall
J L
JL
Boyle
M D
MD
D000942:Antigens, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007070:Immunoglobulin A / Q000032:analysis* / Q000302:isolation & purification; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism; D013292:Streptococcus agalactiae / Q000276:immunology*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007070:Immunoglobulin A; D007141:Immunoglobulin Fc Fragments; D001710:Biotin