Comparison between strains of human T lymphotropic virus type I isolated from inhabitants of the Solomon Islands and Papua New Guinea.
Strains of human T lymphotropic virus type I (HTLV-I) isolated from T cell lines (SI-1, SI-3, and SI-5) from three individuals in separate regions of the Solomon Islands were compared with a variant (PNG-1) isolated from a healthy person in Papua New Guinea and a prototype strain from Japan (MT-2). The SI-1, SI-3, and SI-5 cell lines were predominantly CD8+. Expression of gag- and env-encoded virus-specific proteins was detected in SI-1, SI-3, and SI-5 and in MT-2 cells by immunofluorescence and Western immunoblot; gag proteins p19 and p24 were absent in PNG-1 cells. HTLV-1 gag and pol gene sequences were detected in DNA extracted from SI-1, SI-3, and SI-5 cells by polymerase chain reaction; env sequences were not found in SI-5 cells. Genomic Southern analysis of PstI digests of SI-1, SI-3, and SI-5 DNA exhibited the three moderate-sized fragments typical of prototype HTLV-I. By contrast, PstI digests of PNG-1 DNA yielded two larger fragments.
Yanagihara
R
R
Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892.
Nerukar
V R
VR
Ajdukiewicz
A B
AB
D000328:Adult; D000956:Antigens, Viral / Q000276:immunology; D015139:Blotting, Southern; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D005814:Genes, Viral; D015490:HTLV-I Infections / Q000382:microbiology*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure; D006801:Humans; D008542:Melanesia; D008875:Middle Aged; D010219:Papua New Guinea; D010641:Phenotype; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D013045:Species Specificity
D000956:Antigens, Viral