The application of the statistical study in the morphometric characterization of a Colombian strain of Trypanosoma cruzi.
After morphological and histopathological characterization, the Cali strain of T. cruzi isolated from a patient in Cali (Colombia), was also submitted to a morphometrical characterization. For this we estimated 16 cellular parameters in 30 samples of each evolutive form of T. cruzi in blood in NNN medium through planimetry by the IBAS-1 (Image Basic Analytic System). This morphometrical study was completed with a statistical one through the application of the variance (ANOVA-1). This made it possible to evidence a great number of significant differences between the slender, broad and stout forms and also between the short and large epimastigotes from the NNN medium. These data have a real interest for classifying some features of the biological cycle of T. cruzi as well as the future development of diagnosis techniques or therapeutical and prophylactic experiments.
De Diego
J A
JA
Dpto. de Medicina Preventiva y Salud Pública, Facultad de Medicina, Universidad Autónoma de Madrid, España.
Penin
P
P
Mayer
F
F
Delrey
J
J
Gamallo
C
C
D000704:Analysis of Variance; D000818:Animals; D006801:Humans; D013030:Spain; D014349:Trypanosoma cruzi / Q000302:isolation & purification* / Q000648:ultrastructure