The Id gene is activated by serum but is not required for de-differentiation in rat vascular smooth muscle cells.
Primary rat vascular smooth muscle cells cultured on fibronectin in the absence of serum lost smooth-muscle-specific myosin heavy chain but did not enter the cell cycle and proliferate until they were stimulated by serum. Under these conditions accumulation of Id mRNA occurred only in response to serum and was maximal during the G1 phase of the cycle.
Kemp
P R
PR
Department of Biochemistry, University of Cambridge, U.K.
Grainger
D J
DJ
Shanahan
C M
CM
Weissberg
P L
PL
Metcalfe
J C
JC
D000818:Animals; D001011:Aorta / Q000166:cytology; D001769:Blood; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media; D004261:DNA Replication; D005786:Gene Expression Regulation; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D009218:Myosins / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D003470:Culture Media; D009218:Myosins