title: Characterization of glucosylceramide from plasma membranes of plant root cells.
abstract: Plasma membranes of oat root cells were isolated from intracellular membranes by subfractionation of the microsomal fraction using an aqueous polymer two-phase system. The plasma membranes originated from oat plants which were acclimated to dehydration by exposure to a repeated water-deficit stress program. Glucosylceramides was a major component of the plasma membrane lipids and amounted to 9% of the lipid of control plants and 5% of the lipid of acclimated plants. Structural analysis using FAB-MS showed only one type of glucosylceramides. The constituent monosaccharide was exclusively glucose and the sphingosine base was 4,8-sphingadienine. The fatty acid composition was determined to 24:1-OH, with only trace levels of non-hydroxy acids. The decrease in the level of glucosylceramides during acclimation to dehydration was accompanied by a corresponding decrease in phospholipids and increase in free sterols.
authors.affiliation: Department of Plant Physiology, University of Göteberg, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Norberg
authors.forename: J E
authors.initials: JE
authors.lastname: Månsson
authors.forename: C
authors.initials: C
authors.lastname: Liljenberg
mesh_terms: D002462:Cell Membrane / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002523:Edible Grain / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005947:Glucose / Q000032:analysis; D005963:Glucosylceramides / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry; D008861:Microsomes / Q000737:chemistry; D010944:Plants / Q000032:analysis*; D013110:Sphingosine / Q000032:analysis
chemical_list: D005227:Fatty Acids; D005963:Glucosylceramides; D008563:Membrane Lipids; D005947:Glucose; D013110:Sphingosine