title: Competitive effect of anti-HLA class I monoclonal antibody (01.65) and N-N-staurosporine on prolipherative response of PHA activated T-lymphocytes.
abstract: N-N-Staurosporine (STAR) inhibits in a dose dependent manner Tritiated-Thymidine (3H-TdR) incorporation in phytohemagglutinin (PHA) activated Peripheral Blood Mononuclear Cells (PBMC) treated with anti-HLA class I monoclonal antibody (MAb) 01.65 and its effect results competitive with MAb 01.65. Cytosolic and particulate Protein Kinase C (PKC) have been studied. Only when PKC particulate activity is no more detectable, the effect of STAR on 3H-TdR incorporation is evident.
authors.affiliation: Istituto di Genetica Medica, Università di Ferrara, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Selvatici
authors.forename: P
authors.initials: P
authors.lastname: Orlando
authors.forename: M
authors.initials: M
authors.lastname: Rubini
authors.forename: A
authors.initials: A
authors.lastname: Balboni
authors.forename: S
authors.initials: S
authors.lastname: Balugani
authors.forename: E
authors.initials: E
authors.lastname: Gandini
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D002455:Cell Division; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004306:Dose-Response Relationship, Immunologic; D006680:HLA Antigens / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors* / Q000378:metabolism; D019311:Staurosporine; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
chemical_list: D000470:Alkaloids; D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D010835:Phytohemagglutinins; D011493:Protein Kinase C; D019311:Staurosporine