Molecular cloning and expression of human Ca(2+)-sensitive cytosolic phospholipase A2.
Phospholipases A2 (PLA2s) play a key role in inflammatory processes through production of precursors of eicosanoids and platelet-activating factor. Recently, we described the purification of a novel approximately 100-kDa cytosolic PLA2 (cPLA2) from human monoblast U937 cells that is activated by physiological (intracellular) concentrations of Ca2+ (Kramer, R. M., Roberts, E. F., Manetta, J., and Putnam, J. E. (1991) J. Biol. Chem. 266, 5268-5272). Here we report the isolation of the complementary DNA encoding human cPLA2 and confirm its identity by expression in bacteria and in hamster cells. The predicted 749-amino acid cPLA2 protein has no similarity to the well known secretory PLA2s, but contains a structural element homologous to the C2 region of protein kinase C. The molecular cloning of cPLA2 will allow further studies defining the structure, function, and regulation of this novel PLA2.
Sharp
J D
JD
Lilly Research Laboratories, Indianapolis, Indiana 46285.
White
D L
DL
Chiou
X G
XG
Goodson
T
T
Gamboa
G C
GC
McClure
D
D
Burgett
S
S
Hoskins
J
J
Skatrud
P L
PL
Sportsman
J R
JR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism*; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000235:genetics* / Q000378:metabolism; D054467:Phospholipases A2; D010957:Plasmids; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment
D012333:RNA, Messenger; D004247:DNA; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium