Immunobinding assay for the speciation of avian mycoplasmas adapted for use with a 96-well filtration manifold.
An immunobinding assay capable of distinguishing among Mycoplasma synoviae, M. gallisepticum, M. gallopavonis, and M. meleagridis was developed. A low-protein-binding membrane filter served as the solid support, and a 96-well microsample filtration manifold was used. The assay detected 50 ng of mycoplasmal protein, or approximately 3 x 10(4) colony-forming units, from pure or mixed cultures. No cross-reactions were observed among the mycoplasma species tested. The assay was inexpensive, easy to interpret, and required approximately 3 hr to perform. The 96-well format allowed screening of numerous cultures and use of several antisera in a single assay.
Sharp
P
P
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706.
Van Ess
P
P
Ji
B
B
Thomas
C B
CB
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D003429:Cross Reactions; D003720:Densitometry; D005374:Filtration; D007106:Immune Sera / Q000276:immunology; D007118:Immunoassay; D009174:Mycoplasma / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D011237:Predictive Value of Tests
D000942:Antigens, Bacterial; D007106:Immune Sera