title: Postnatal changes in lambs of two pathways for thyroxine 5'-monodeiodination in brown adipose tissue.
abstract: We have recently shown that ovine fetal brown adipose tissue (BAT) contains two distinct iodothyronine 5'-monodeiodinase (5'MDI) activities, one with a high Km (type I) and another with a low Km (type II). Both activities increased to maximum levels near term (150 days gestation). BAT plays a major role in neonatal temperature regulation in lambs, and available evidence suggests that BAT 5'MDI activity is closely linked to thermogenic capacity. To better characterize the changes in 5'MDI after birth, we studied both type I and type II 5'MDI in lamb BAT from the time of birth to 30 days of postnatal age. Type I 5'MDI activity [pmol 3,5,3'-triiodothyronine (T3).mg protein-1.h-1] showed no significant changes during the first 11 days after birth [newborn (NB), 95 +/- 16; 1 day, 83 +/- 20; 3-4 days, 80 +/- 11; 10-11 days, 92 +/- 28]. Activity decreased significantly at 30 days (24 +/- 8.9, P less than 0.05). On the other hand, the type II 5'MDI activity (fmol I- released.mg protein-1.h-1) increased significantly (P less than 0.01) during the first 4 days, (NB, 348 +/- 23; 1 day, 679 +/- 37; 3-4 days, 785 +/- 199), decreased toward NB values (401 +/- 87) at 10-11 days of age, and fell to 66 +/- 31 at 30 days (P less than 0.05 vs. NB). Kinetic analysis of BAT type II thyroxine 5'MDI revealed a rise in maximum velocity from NB to 1 and 3-4 days of age without a change in the enzymatic activity Km.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Nuclear Medicine and Medical Services, Veteran Administration Medical Center, Long Beach 90822.
authors.forename: S Y
authors.initials: SY
authors.lastname: Wu
authors.forename: J K
authors.initials: JK
authors.lastname: Kim
authors.forename: I J
authors.initials: IJ
authors.lastname: Chopra
authors.forename: Y
authors.initials: Y
authors.lastname: Murata
authors.forename: D A
authors.initials: DA
authors.lastname: Fisher
mesh_terms: D002001:Adipose Tissue, Brown / Q000033:anatomy & histology / Q000201:enzymology*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development*; D001835:Body Weight; D007453:Iodide Peroxidase / Q000145:classification / Q000378:metabolism*; D007668:Kidney; D007700:Kinetics; D009929:Organ Size; D009994:Osmolar Concentration; D011506:Proteins / Q000378:metabolism; D012756:Sheep; D014284:Triiodothyronine / Q000097:blood
chemical_list: D011506:Proteins; D014284:Triiodothyronine; D007453:Iodide Peroxidase