Lytic activity from schistosomes: interactions with blood cells.
1. The schistosome lytic agent hemolyzed animal red blood cells (RBCs) containing high concentrations of membrane phosphatidyl choline (dog, mouse, and rat) more efficiently than RBCs having no phosphatidyl choline (goat and sheep). 2. Human mononuclear cells lost viability in the presence of the schistosome lytic agent. 3. Preincubating the lytic agent with phosphatidyl choline or bovine serum albumin reduced its lytic activity. 4. Extracellular albumin protected the RBCs from schistosome induced hemolysis. 5. Pretreatment of the RBCs with various proteases enhanced lysis by 10-30%.
Kasschau
M R
MR
Department of Biological and Allied Health Sciences, University of Houston-Clear Lake, TX 77058.
Gentry
D L
DL
Byam-Smith
M P
MP
D000017:ABO Blood-Group System; D000818:Animals; D006224:Cricetinae; D004285:Dogs; D004910:Erythrocyte Membrane / Q000502:physiology; D005260:Female; D006041:Goats; D006461:Hemolysis; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000502:physiology; D008297:Male; D008647:Mesocricetus; D051379:Mice; D010713:Phosphatidylcholines / Q000494:pharmacology; D051381:Rats; D012550:Schistosoma mansoni / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D012756:Sheep; D013045:Species Specificity
D000017:ABO Blood-Group System; D010713:Phosphatidylcholines; D012710:Serum Albumin, Bovine