title: The primary structure of glucagon-like peptide but not insulin has been conserved between the American eel, Anguilla rostrata and the European eel, Anguilla anguilla.
abstract: Insulin was isolated from the pancreas of the American eel, Anguilla rostrata, and its primary structure was established as (Formula: see text). Eel insulin contains unusual substitutions at B-21, B-22, and B-26 in the putative receptor-binding region of the molecule compared with other mammalian and fish insulins. The A-chain of insulin from the European eel contains an asparagine rather than a serine residue at position A-12. Similarly, amino acid composition data indicate the B-chain of insulin from the European eel is appreciably different from that from the American eel. The primary structure of glucagon-like peptide (GLP) from the American eel is identical to that from the European eel, Anguilla anguilla. The primary structure of the peptide was established as (Formula: see text). Fast-atom bombardment mass spectrometry demonstrated that the COOH-terminal arginyl residue is alpha-amidated. The strong evolutionary pressure to conserve the structure of GLP provides further support for the assertion that the peptide plays an important regulatory role in teleost fish.
authors.affiliation: Department of Biomedical Sciences, Creighton University Medical School, Omaha, Nebraska 68178.
authors.forename: J M
authors.initials: JM
authors.lastname: Conlon
authors.forename: P C
authors.initials: PC
authors.lastname: Andrews
authors.forename: L
authors.initials: L
authors.lastname: Thim
authors.forename: T W
authors.initials: TW
authors.lastname: Moon
mesh_terms: D000595:Amino Acid Sequence; D000811:Anguilla; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004763:Glucagon-Like Peptides; D007328:Insulin / Q000737:chemistry* / Q000302:isolation & purification; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010179:Pancreas / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D013045:Species Specificity; D014357:Trypsin
chemical_list: D007328:Insulin; D010446:Peptide Fragments; D010455:Peptides; D004763:Glucagon-Like Peptides; C015236:glucagon-like-immunoreactivity; D014357:Trypsin