title: Antibody to Pseudomonas pseudomallei exotoxin in sheep exposed to natural infection.
abstract: Specific antibody to Pseudomonas pseudomallei exotoxin was detected in sheep sera exposed to natural infection. An enzyme-linked immunosorbent assay (ELISA) was used. Serum antitoxin was present in 49.3% of sera obtained from a flock of sheep naturally exposed to P. pseudomallei infection. Among these sera, 17.0% gave titers of 10,000. In contrast, serum antitoxin was present in only 6.0% of sera collected from sheep kept on a melioidosis-free farm. The ELISA reactivity of all positive sera could be completely absorbed with purified P. pseudomallei exotoxin. Similarly, preincubation of the exotoxin-coated wells with specific antiserum inhibited the ELISA reactivity of sheep sera. The results indicate that exotoxin is produced in vivo during infection by P. pseudomallei.
authors.affiliation: Faculty of Science and Natural Resources, Universiti Kebangsaan, Sabah, Malaysia.
authors.forename: G
authors.initials: G
authors.lastname: Ismail
authors.forename: R
authors.initials: R
authors.lastname: Mohamed
authors.forename: S
authors.initials: S
authors.lastname: Rohana
authors.forename: H S
authors.initials: HS
authors.lastname: Sharifah
authors.forename: N
authors.initials: N
authors.lastname: Embi
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000992:Antitoxins / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005098:Exotoxins / Q000276:immunology*; D008554:Melioidosis / Q000276:immunology / Q000662:veterinary*; D011549:Pseudomonas / Q000276:immunology*; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000992:Antitoxins; D005098:Exotoxins