title: Detection of the human immunodeficiency virus genome with a biotinylated DNA probe generated by polymerase chain reaction.
abstract: A non-radiolabelled DNA probe was developed for detection of the human immunodeficiency virus type 1 (HIV-1) genome using the polymerase chain reaction (PCR) technology. Primers amplifying a 395 base pair segment of a portion of the polymerase region of the HIV-1 genome were used both to amplify sample target DNA and to generate a biotinylated DNA probe used in Southern blot hybridization. This probe performed as well as one produced by nick translation using biotinylated nucleotides or an enzyme labelled oligonucleotide probe.
authors.affiliation: Viral and Rickettsial Disease Laboratory, California State Department of Health Services, Berkeley 94704.
authors.forename: B
authors.initials: B
authors.lastname: Forghani
authors.forename: J W
authors.initials: JW
authors.lastname: Hurst
authors.forename: G R
authors.initials: GR
authors.lastname: Shell
mesh_terms: D001426:Bacterial Proteins; D001483:Base Sequence; D001710:Biotin; D015152:Blotting, Northern; D015139:Blotting, Southern; D015342:DNA Probes; D015751:Genes, pol / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D019809:Streptavidin
chemical_list: D001426:Bacterial Proteins; D015342:DNA Probes; D001710:Biotin; D019809:Streptavidin