title: Transformation of 3-chlorodibenzofuran by Pseudomonas sp. HH69.
abstract: The dibenzofuran-degrading bacterial strain Pseudomonas sp. HH69 showed high oxidative activity towards 3-chlorodibenzofuran (3CDF). During the co-metabolic turnover of 3CDF large amounts of 4-chlorosalicylate and temporarily small amounts of salicylate were excreted. Simultaneously a yellow colour appeared due to the excretion of two polar products. Conversion of 3CDF by a mutant, derived from Pseudomonas sp. HH69 and defective in 2,3-dihydroxybiphenyl-1,2-dioxygenase led to the formation of equal quantities of 4'-chloro-2,2',3-trihydroxybiphenyl (4'CTHBP) and 4-chloro-2,2',3-trihydroxybiphenyl (4CTHBP). Crude extracts of the wild type transformed 4'CTHBP to 4-chlorosalicylate, whilst 4CTHBP was transformed to salicylate. Hence, we propose a non-selective initial attack on both aromatic rings of 3CDF and a degradative pathway for the resulting chlorotrihydroxybiphenyls.
authors.affiliation: Abteilung Mikrobiologie, Universität Hamburg, F.R.G.
authors.forename: H
authors.initials: H
authors.lastname: Harms
authors.forename: H
authors.initials: H
authors.lastname: Wilkes
authors.forename: V
authors.initials: V
authors.lastname: Sinnwell
authors.forename: R M
authors.initials: RM
authors.lastname: Wittich
authors.forename: K
authors.initials: K
authors.lastname: Figge
authors.forename: W
authors.initials: W
authors.lastname: Francke
authors.forename: P
authors.initials: P
authors.lastname: Fortnagel
mesh_terms: D001572:Benzofurans / Q000493:pharmacokinetics*; D001711:Biotransformation; D011549:Pseudomonas / Q000378:metabolism*
chemical_list: D001572:Benzofurans; C068119:3-chlorodibenzofuran