Recombinant human MCP-1/JE induces chemotaxis, calcium flux, and the respiratory burst in human monocytes.
The JE gene was first described as a platelet-derived growth factor (PDGF)-inducible gene in mouse 3T3 cells. The human homologue of JE encodes a protein whose predicted amino acid sequence is identical to that of the monocyte chemoattractant MCP-1 (also called MCAF and SMC-CF), which belongs to a recently identified family of small secretory proteins with cytokine properties. We purified recombinant human MCP-1/JE (hMCP-1/JE) produced in COS cells and demonstrated that it is chemotactic for human monocytes with a specific activity similar to natural MCP-1. In addition, pure recombinant hMCP-1/JE stimulates monocytes, inducing an increase in cytosolic free calcium and the respiratory burst, but is completely inactive on human neutrophils. These results help to define functionally a well-known growth factor-inducible gene and a member of a new family of cytokines.
Rollins
B J
BJ
Division of Medicine, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts.
Walz
A
A
Baggiolini
M
M
D000818:Animals; D002118:Calcium / Q000097:blood*; D002460:Cell Line; D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood; D008163:Luminescent Measurements; D051379:Mice; D009000:Monocytes / Q000502:physiology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009247:NADH, NADPH Oxidoreductases / Q000097:blood; D019255:NADPH Oxidases; D010101:Oxygen Consumption; D011994:Recombinant Proteins / Q000494:pharmacology; D014162:Transfection
D018932:Chemokine CCL2; D002630:Chemotactic Factors; D011994:Recombinant Proteins; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006861:Hydrogen Peroxide; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D002118:Calcium