title: Efficient gene transfer and expression in primary B lymphocytes.
abstract: In this study we have used a panel of vectors expressing the chloramphenicol acetyltransferase (CAT) reporter gene under the control of different regulatory elements to optimize gene transfer and expression in primary B lymphocytes. The Moloney murine leukemia virus long terminal repeat (MoMLV LTR) and the SV40 early region promoters, while functional in transfected plasmacytoma cell lines, did not give rise to detectable CAT activity following transfection into primary activated mouse or human B lymphocytes. In contrast, the human cytomegalovirus immediate-early (HCMV-IE) enhancer/promoter functioned in both established and primary B cells. The highest expression levels in the primary cells were obtained with vectors containing the Adenovirus 2 major late promoter or the HCMV-IE enhancer/promoter in combination with the Adenovirus 2 tripartite leader and VA genes. These latter expression cassettes were placed in a retroviral vector with the aim of combining their capacity for high-level gene expression with the efficient stable gene transfer afforded by retroviral infection. Several retroviral constructs were made, some of which were able to generate high virus titers. However all of these underwent deletions during the process of retroviral infection, as judged by Southern analysis of infected cells, indicating that they were not optimal gene transfer vectors. The HCMV enhancer/promoter, which was the most active of the other expression cassettes tested in the primary B cells, was inserted into a retroviral vector which also expressed the hph gene under the transcriptional control of the retroviral LTR. This vector did not undergo rearrangement during the process of retroviral infection, as judged by Southern analysis. The CAT gene was inserted downstream of the HCMV promoter in this vector, and a high-titer retroviral stock was generated. Primary B lymphocytes infected with this vector gave high levels of CAT activity, under conditions in which parallel experiments with the hph drug resistance marker showed that one in 20 of the cells were infected. These experiments demonstrate efficient gene transfer and expression in primary B lymphocytes in vitro.
authors.affiliation: Department of Molecular Biology, Immunex Corporation, Seattle, WA 98101.
authors.forename: R W
authors.initials: RW
authors.lastname: Overell
authors.forename: K E
authors.initials: KE
authors.lastname: Weisser
authors.forename: B W
authors.initials: BW
authors.lastname: Hess
authors.forename: S F
authors.initials: SF
authors.lastname: Ziegler
authors.forename: C M
authors.initials: CM
authors.lastname: Pleiman
authors.forename: C
authors.initials: C
authors.lastname: Maliszewski
authors.forename: K H
authors.initials: KH
authors.lastname: Grabstein
mesh_terms: D000818:Animals; D001402:B-Lymphocytes; D002460:Cell Line; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D010957:Plasmids; D012190:Retroviridae / Q000235:genetics; D014162:Transfection
chemical_list: D015500:Chloramphenicol O-Acetyltransferase