Determination of diltiazem hydrochloride enantiomers in dog plasma using chiral stationary-phase liquid chromatography.
The separation and determination of d- and l-diltiazem hydrochloride in dog plasma by a two-column high-performance liquid chromatographic technique are described. Diltiazem hydrochloride and its metabolites were extracted from dog plasma and analyzed on a conventional column (Nucleosil 5C18) with a volatile buffer system. The column effluent of diltiazem hydrochloride was collected and evaporated. The enantiomeric ratio of the collected diltiazem was determined using a chiral column (Chiralcel OC). The method was accurate and sensitive.
Ishii
K
K
Analytical Chemistry Research Laboratory, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
Banno
K
K
Miyamoto
T
T
Kakimoto
T
T
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004110:Diltiazem / Q000097:blood* / Q000737:chemistry; D004285:Dogs; D015394:Molecular Structure; D013237:Stereoisomerism
D004110:Diltiazem