title: Transfer of resistance determinants from a multi-resistant Staphylococcus aureus isolate.
abstract: The clinical isolate Staphylococcus aureus WBG1024 was resistant to cadmium, benzyl penicillin, kanamycin, neomycin, streptomycin, tetracycline and trimethoprim and harboured a conjugative plasmid pWBG637 (34.5 kb) and non-conjugative plasmids of 23.8, 4.4, 2.8 and 1.9 kb. Transduction and mixed-culture transfer experiments demonstrated that the 4.4-kb plasmid (pWBG632) encoded resistance to tetracycline and the 23.8-kb plasmid (pWBG628) encoded resistance to cadmium, benzyl penicillin, kanamycin, neomycin and streptomycin. The conjugative plasmid pWBG637 was able to mobilise a further 4.4-kb plasmid (pWBG633) encoding streptomycin resistance and recombined with the multiresistance plasmid pWBG628 to produce transconjugantes of various resistance phenotypes.
authors.affiliation: School of Medical Technology, Curtin University of Technology, Perth, Western Australia.
authors.forename: E E
authors.initials: EE
authors.lastname: Udo
authors.forename: W B
authors.initials: WB
authors.lastname: Grubb
mesh_terms: D015139:Blotting, Southern; D002104:Cadmium / Q000494:pharmacology; D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial / Q000235:genetics*; D007612:Kanamycin / Q000494:pharmacology; D009355:Neomycin / Q000494:pharmacology; D010403:Penicillin Resistance / Q000235:genetics; D010957:Plasmids; D011995:Recombination, Genetic; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*; D013307:Streptomycin / Q000494:pharmacology; D013753:Tetracycline Resistance / Q000235:genetics; D014161:Transduction, Genetic; D014295:Trimethoprim / Q000494:pharmacology
chemical_list: D002104:Cadmium; D007612:Kanamycin; D014295:Trimethoprim; D009355:Neomycin; D013307:Streptomycin