A major substrate of maturation promoting factor identified as elongation factor 1 beta gamma delta in Xenopus laevis.
Protein synthesis is believed to be under control of the cell cycle during meiosis and mitosis. Any relationship between substrates for cdc2 kinase and components of the protein synthetic apparatus would therefore be of prime importance. During meiosis of Xenopus laevis oocytes one of the substrates for this kinase is a p47 protein, which is complexed to two other proteins, P36 and P30. Judged from partial amino acid sequence data on P47 and P30, the P30 and P47 proteins were reported to resemble the protein synthetic elongation factors (EF) 1 beta and 1 gamma from Artemia salina (Bellé, R., Derancourt, J., Poulhe, R., Capony, J.P., Ozon, R., and Mulner-Lorillon, O. (1989) FEBS Lett. 255, 101-104). This paper shows that the complex composed of P30, P47, and P36 from Xenopus is identical to the complex of EF-1 beta, EF-1 gamma, and EF-1 delta from Artemia according to two criteria. 1) Both stimulate elongation factor 1 alpha-mediated transfer RNA binding to ribosomes and exchange of guanine nucleotides on elongation factor 1 alpha to a comparable degree. 2) Each of the three subunits of the protein complex P30.P47.P36 from Xenopus shows a structural homology with one of the corresponding subunits of EF-1 beta gamma delta from Artemia. Presumably the phosphorylation of EF-1 gamma, which associates with tubulin at least in vitro, is important in processes following the onset of meiosis which is accompanied by a rise of protein synthesis.
Janssen
G M
GM
Department of Medical Biochemistry, Sylvius Laboratory, State University of Leiden, The Netherlands.
Morales
J
J
Schipper
A
A
Labbé
J C
JC
Mulner-Lorillon
O
O
Bellé
R
R
Möller
W
W
D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia; D016203:CDC2 Protein Kinase / Q000378:metabolism; D015977:Eukaryotic Initiation Factor-1 / Q000378:metabolism*; D007525:Isoelectric Focusing; D016200:Maturation-Promoting Factor / Q000378:metabolism*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D016415:Sequence Alignment; D013379:Substrate Specificity; D014982:Xenopus laevis
D015977:Eukaryotic Initiation Factor-1; D016203:CDC2 Protein Kinase; D016200:Maturation-Promoting Factor