title: Induction of metallothionein and stimulation of calcification by dexamethasone in cultured clonal osteogenic cells, MC3T3-E1.
abstract: To clarify the effects of dexamethasone (Dex) on metallothionein (MT) synthesis and calcification in osteoblastic cells, a clonal osteogenic cell line (MC3T3-E1) was used in the present study. Under culture conditions designed not to cause calcification, MT synthesis of cells at 3 days after inoculation increased with increasing concentration of Dex (2.5-50 nM) for a 24-h culture period. Cells at 6 or 9 days after inoculation also synthesized MT by a 24-h exposure to Dex. These show that undifferentiated osteoblasts have the ability to synthesize MT by Dex. Under culture conditions designed to cause calcification, cells at 6 days after inoculation were cultured with 50 nM Dex in the presence of 7 mM beta-glycerophosphate (beta-GP) for 7 days. Ca content of Dex-treated cells was about 7.5 times as high as that of untreated cells. Dex-treated cells showed a high activity of alkaline phosphatase (ALP). The Zn content of the MT fraction in Dex-treated cells was about 8 times as high as that of untreated cells. These results show that Dex has the ability to induce MT synthesis in osteoblastic cells and to cause a high calcification which is due at least partly to an enhanced activity of ALP.
authors.affiliation: Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Miyahara
authors.forename: M
authors.initials: M
authors.lastname: Nemoto
authors.forename: S
authors.initials: S
authors.lastname: Tukamoto
authors.forename: H
authors.initials: H
authors.lastname: Yamada
authors.forename: H
authors.initials: H
authors.lastname: Kozuka
authors.forename: S
authors.initials: S
authors.lastname: Kuze
authors.forename: H
authors.initials: H
authors.lastname: Sudo
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D002113:Calcification, Physiologic / Q000187:drug effects*; D002454:Cell Differentiation; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D003907:Dexamethasone / Q000494:pharmacology*; D008668:Metallothionein / Q000096:biosynthesis*; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D015032:Zinc / Q000378:metabolism
chemical_list: D003907:Dexamethasone; D004247:DNA; D008668:Metallothionein; D000469:Alkaline Phosphatase; D015032:Zinc