A simple method for cloning Giardia duodenalis from cultures and fecal samples.
Using a novel method for cloning Giardia duodenalis from cultures and fecal samples, 47 clones from 7 isolates were established in vitro. Average colony-forming efficiency in established cultures was 43.2% compared to 11.2% when cloning directly from excystation. The highest success rate of cloning was found with the Portland (P1, ATCC No. 30888) isolate, with a colony-forming efficiency of 92.7%. Cloned and parent populations were compared over a range of 13 enzymes using starch gel electrophoresis. No genetic difference was found between any of the clones and the parent isolates.
Binz
N
N
Division of Veterinary Biology, School of Veterinary Studies, Murdoch University, Western Australia.
Thompson
R C
RC
Meloni
B P
BP
Lymbery
A J
AJ
D000818:Animals; D005243:Feces / Q000469:parasitology*; D005872:Giardia / Q000254:growth & development*; D008722:Methods