title: Rhinosporidium seeberi: light, phase contrast, fluorescent and scanning electron microscopic study.
abstract: Phase contrast microscopic study indicated the multilayered structure of the sporangial wall of R. seeberi while the scanning electronmicroscopic study revealed a trilaminated wall compared to a thick double walled light microscopic structure. The scanning electronmicroscopy revealed the spores of varying sizes which were found either discretely or in groups interconnected and seen attached to the inner aspect of the sporangial wall. Autofluorescence of sporangia and spores was observed under microscope. Acridine orange staining revealed the presence of DNA materials in the spore and sporangia.
authors.affiliation: Madras Veterinary College, India.
authors.forename: J S
authors.initials: JS
authors.lastname: Moses
authors.forename: C
authors.initials: C
authors.lastname: Balachandran
authors.forename: B
authors.initials: B
authors.lastname: Singh
authors.forename: A
authors.initials: A
authors.lastname: Shanmugan
authors.forename: D
authors.initials: D
authors.lastname: Moses
mesh_terms: D000165:Acridine Orange; D000818:Animals; D005629:Frozen Sections; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D012228:Rhinosporidium / Q000166:cytology / Q000648:ultrastructure*; D013172:Spores, Fungal / Q000648:ultrastructure
chemical_list: D000165:Acridine Orange