Alpha/beta inversion of the Euplotes and Oxytricha tubulins.
Immunoblotting studies, using a polyclonal antibody specific for the alpha-tubulin of ciliates and an anti-beta-tubulin monoclonal antibody, demonstrated that the tubulins of the ciliates Euplotes and Oxytricha show alpha/beta inversion although less accentuated than that observed in Paramecium and Tetrahymena. Results suggest that (a) the alpha/beta inversion may be a common characteristic within the Phylum Ciliophora; and (b) the electrophoretic behaviour of the alpha-tubulin may be useful for establishing evolutionary relatedness between the ciliates.
Delgado
P
P
Departamento de Microbiologia, Facultad de Biologia, Universidad de Sevilla, Spain.
Romero
M R
MR
Torres
A
A
D000818:Animals; D000918:Antibody Specificity; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera; D015151:Immunoblotting; D010247:Paramecium / Q000032:analysis / Q000378:metabolism*; D013768:Tetrahymena / Q000032:analysis / Q000378:metabolism*; D014404:Tubulin / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*
D007106:Immune Sera; D014404:Tubulin