Hepatic intracellular pH in vivo using F-quene 1 and 19F NMR spectroscopy.
Intracellular pH (pHi) has been determined in vivo in livers from anaesthetized rats using both 31P NMR and 19F NMR spectroscopy. In the 31P NMR study pHi, determined from the chemical shift of endogenous Pi, was found to be 7.26 +/- 0.02. In the 19F NMR study on a separate group of animals pHi was determined from the chemical shift of F-Quene 1 infused via the portal vein and found to be 7.18 +/- 0.01. In approximately half of the 19F NMR studies no 19F signal could be detected in the liver probably because of the two probes could be caused by different intra- or intercellular distributions or by unknown effects of F-Quene 1 on metabolism.
Beech
J S
JS
Medical Unit (Cellular Mechanisms Research Group), London Hospital Medical College, Whitechapel.
Iles
R A
RA
D000634:Aminoquinolines / Q000378:metabolism; D000818:Animals; D005456:Fluorescent Dyes; D005461:Fluorine; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010758:Phosphorus; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000634:Aminoquinolines; D005456:Fluorescent Dyes; C070618:F-Quene 1; D010758:Phosphorus; D005461:Fluorine; C038108:8-(bis(ethoxycarbonylmethyl)amino)-6-methoxy-2-(bis(ethoxycarbonylmethyl)amino)styrylquinoline