High-performance liquid chromatographic analysis of 5-ethylpyrimidines and 5-methylpyrimidines in plasma.
A high-performance liquid chromatographic (HPLC) method employing a C18 reversed-phase column, a mobile phase of sodium acetate and methanol, and an ultraviolet detector was developed for the analysis of 5-ethylpyrimidines and 5-methylpyrimidines in plasma. Samples were prepared for HPLC by sequential cation-exchange and anion-exchange column chromatography. Linear standard curves were obtained for samples containing 0.05-50 micrograms/ml 5-ethyl-2'-deoxyuridine and 5-ethyluracil, 0.05-10 micrograms ml 5-(1-hydroxyethyl)uracil, and 0.1-50 micrograms/ml thymidine, thymine and 5-hydroxymethyluracil. Applicability of the method to determination of the kinetics of 5-ethyl-2'-deoxyuridine elimination by the isolated perfused rat liver was demonstrated; clearance of the drug was 1.29 ml/min.
Joly
J M
JM
Department of Pharmacology and Toxicology, University of Louisville School of Medicine, KY 40292.
Williams
W M
WM
D000818:Animals; D000998:Antiviral Agents / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D013936:Thymidine / Q000097:blood*; D013941:Thymine / Q000097:blood; D014498:Uracil / Q000031:analogs & derivatives / Q000097:blood
D000998:Antiviral Agents; C022811:edoxudin; C048011:5-ethyluracil; D014498:Uracil; D013941:Thymine; D013936:Thymidine; D003857:Deoxyuridine