[Rearrangements of yeast chromosomes revealed by pulsed field gel electrophoresis].
Electrophoretic karyotypes of yeast Saccharomyces cerevisiae integrant strains containing the pYF91 plasmid integrated into the chromosomes I, III, VI, IX, XI were studied. A possibility was demonstrated of visual identification of the chimaeric chromosomes via the molecular weight increase by 13200 bp (the plasmid size) determined by pulsed field gel electrophoresis. Several gamma-rays induced rearrangements of the yeast chimaeric chromosome I causing instability in hybrids were also studied. The deletions induced in the I chromosome were analysed and their size estimated. The technique of pulsed field gel electrophoresis is recommended for determination of insertions and deletions in the chromosomes of yeast Saccharomyces cerevisiae.
Iarovoĭ
B F
BF
Mezhevaia
E v
Ev
Stepanova
V L
VL
Zakharov
I A
IA
D002872:Chromosome Deletion; D015825:Chromosomes, Fungal / Q000502:physiology*; D004586:Electrophoresis / Q000379:methods; D015321:Gene Rearrangement / Q000235:genetics*; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000235:genetics*
Perestroĭki drozhzhevykh khromosom, vyiavliaemye metodom pul'soforeza.