Pathogenesis of experimental combined infections with Isospora suis and rotavirus in conventional and gnotobiotic piglets.
51 gnotobiotic and 63 conventional, one-, or two-days-old piglets were divided into five groups and infected orally either with Isospora suis or rotavirus alone, or with both agents simultaneously or successively with alternative sequences and various intervals. 15 gnotobiotic and 10 conventional piglets served as controls. The development of small intestinal lesions after infection with I. suis was biphasic. The dominant alteration resulting from rotavirus infection was villus atrophy, considerably more pronounced and extensive in gnotobiotic than in conventional piglets. Synergistic action of I. suis and rotavirus was manifested both clinically, and morphologically. This action culminated at the time of the actual, or presumed development of merogony of I. suis, i.e. on DPI 3 to 5. The action develops only if the intestinal epithelium is damaged functionally and morphologically by a preceding rotavirus infection. It is concluded that the synergistic action is based on a competition of rotavirus and I. suis for mature, enzymatically active absorptive cells.
Vítovec
J
J
Institut of Parasitology, Czechoslovak Academy of Sciences, Ceské Budĕjovice.
Koudela
B
B
Kudweis
M
M
Stĕpánek
J
J
Smíd
B
B
Dvorák
R
R
D000818:Animals; D003048:Coccidiosis / Q000150:complications / Q000473:pathology / Q000662:veterinary*; D005856:Germ-Free Life; D007422:Intestines / Q000473:pathology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D012400:Rotavirus Infections / Q000150:complications / Q000473:pathology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000473:pathology*