title: Lymphocyte subsets in bone marrow lymphoid nodules and malignant lymphoma nodular involvement.
abstract: The lymphocyte subsets in 67 cases of bone marrow benign or reactive lymphoid nodules (LN) and 23 cases of nodular involvement by B malignant lymphoma (B-ML) have been immunohistochemically characterized on paraffin embedded trephine biopsies utilizing a panel of 10 monoclonal antibodies. LCA was positive in 90% LN and B-ML lymphocytes; LN2, MB2 stained more than 50% LN and B-ML lymphocytes; MT2 stained more than 50% LN and less than 50% B-ML lymphocytes; UCHL1 stained the 20% of LN lymphocytes; LN1 stained only B cells of the rare germinal centers; MT1 only myeloid cells, L26 only plasma cells. DF-T1 and Leu 22 failed to stain LN or B-ML lymphocytes. While anti-T lymphocyte antibodies reacted inconstantly with lymphoid cells in decalcified and embedded specimens, LCA and some anti-B lymphocyte antibodies gave constantly reproducible results for bone marrow LN and B-ML. They permitted an easy recognition and exact evaluation of the size of LN, the identification of scattered B cells, the detection of the residual or minimal involvement by B-ML and the exact burden of the invasion, but could not allow a convincing differential diagnosis between LN and small cell B-ML nodular involvement.
authors.affiliation: Dipartimento di Scienze Biomediche e Oncologia Umana dell'Università di Torino, Italia.
authors.forename: A
authors.initials: A
authors.lastname: Pich
authors.forename: M
authors.initials: M
authors.lastname: Gastaldi
authors.forename: G
authors.initials: G
authors.lastname: Tragni
authors.forename: R
authors.initials: R
authors.lastname: Navone
mesh_terms: D000911:Antibodies, Monoclonal; D001853:Bone Marrow / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000473:pathology*; D016131:Lymphocyte Subsets
chemical_list: D000911:Antibodies, Monoclonal