Stimulation of airway ciliary motility by immunologically activated canine pulmonary macrophages: role of leukotrienes.
To elucidate a possible interaction between alveolar macrophages and airway epithelial cells in allergic conditions, we studied the effect of immunologically stimulated macrophages on ciliary beat frequency (CBF) of cultured canine tracheal epithelium by a photoelectric method. Administration of supernatants from macrophages incubated with anti-dinitrophenyl (DNP) IgE antibody and anti-dinitrophenyl-human serum albumin dose-dependently increased ciliary beat frequency, the maximal increase from the baseline being 30.4 +/- 5.0% (mean +/- SE, P less than 0.01), an effect that was accompanied by the release of leukotriene (LT) C4 and leukotriene D4. This ciliostimulation was not affected by pretreatment of macrophages with indomethacin but was inhibited by that with nordihydroguaiaretic acid. Addition of FPL 55712 abolished the response of ciliary beat frequency to the stimulated macrophages, and exogenously administered leukotriene C4 and leukotriene D4 dose-dependently increased ciliary beat frequency. These results suggest that macrophages increase respiratory ciliary motility through the IgE-mediated release of leukotrienes and may modulate mucociliary transport function in the airway.
Tamaoki
J
J
First Department of Medicine, Tokyo Women's Medical College, Japan.
Sakai
N
N
Kobayashi
K
K
Kanemura
T
T
Takizawa
T
T
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D002923:Cilia / Q000502:physiology; D004285:Dogs; D007073:Immunoglobulin E / Q000276:immunology; D007213:Indomethacin / Q000494:pharmacology; D015289:Leukotrienes / Q000502:physiology*; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology / Q000502:physiology*; D009637:Masoprocol / Q000494:pharmacology; D011650:Pulmonary Alveoli / Q000276:immunology / Q000502:physiology*; D013189:SRS-A / Q000502:physiology
D001095:Arachidonic Acids; D015289:Leukotrienes; D013189:SRS-A; D007073:Immunoglobulin E; D009637:Masoprocol; D007213:Indomethacin