Primary dissociated cell culture of embryonic rat metencephalon: presence of GABA in serotonergic neurons.
This study was performed to determine whether neurons, where gamma-aminobutyric acid (GABA) and serotonin (5-HT) coexist, represent neuronal entities which can survive in vitro. In dissociated cultures from 18-day-old embryonic rat metencephalon, it was possible to develop glial and neuronal cells. Among the neurons, some of them, which contain glutamate decarboxylase or are capable of accumulating [3H]GABA are GABAergic; others, containing tryptophan hydroxylase or 5-HT are serotoninergic. By combining radioautography and immunocytochemistry, it was possible to observe neurons where 5-HT and GABA coexist. Cultures might be a suitable model to study the functioning (release or synthesis of both neurotransmitters) of neurons where two classical neurotransmitters coexist.
Belin
M F
MF
INSERM CJF 9010, CNRS URA 1195, Laboratoire d'Anatomie Pathologique, Hôpital Neurologique, Lyons, France.
Fevre-Montange
M
M
Reboul
A
A
Didier-Bazes
M
M
Ehret
M
M
Maitre
M
M
Tardy
M
M
D000818:Animals; D001345:Autoradiography; D001692:Biological Transport; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D007150:Immunohistochemistry; D008858:Microscopy, Phase-Contrast; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D011149:Pons / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D012701:Serotonin / Q000032:analysis*; D014316:Tritium; D005680:gamma-Aminobutyric Acid / Q000032:analysis* / Q000378:metabolism
D014316:Tritium; D012701:Serotonin; D005680:gamma-Aminobutyric Acid