DNA probes for the detection of Babesia caballi.
A genomic library of Babesia caballi DNA was constructed in the plasmid vector pUC13. The specificity of the clones for B. caballi was established by the lack of hybridization to Babesia equi, Babesia bovis, Babesia bigemina and equine DNA. Two probes, pBC11 and pBC191, were isolated that could detect 0.25 ng and 0.125 ng of B. caballi DNA, corresponding to a parasitaemia of 0.12% and 0.06% respectively. pBC191 could detect B. caballi parasites in the blood of an experimentally infected horse as well as in naturally infected horses.
Posnett
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Molecular Biology Section, Veterinary Research Institute, South Africa.
Ambrosio
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D000818:Animals; D001403:Babesia / Q000235:genetics* / Q000302:isolation & purification; D001404:Babesiosis / Q000175:diagnosis*; D015139:Blotting, Southern; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D015723:Gene Library; D005822:Genetic Vectors; D006734:Horse Diseases / Q000175:diagnosis*; D006736:Horses; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011237:Predictive Value of Tests; D015183:Restriction Mapping; D013045:Species Specificity
D015342:DNA Probes; D016054:DNA, Protozoan