Electrophoretic separation of S. pombe chromosomes in polyacrylamide solutions using a constant field.
Previous electrophoretic separations of megabase (Mb) sized DNA have been achieved in pulsed electric fields, using agarose gel as a matrix. The present study demonstrates separations of Mb sized DNA due to a retardation of migration in proportion to the concentration of uncrosslinked polyacrylamide of 5 x 10(6) molecular weight, using a constant electric field. Potentially, the method should be applicable to large DNA in general, greatly reducing the instrumental complexity of such separations and rendering them compatible with capillary electrophoresis apparatus.
Guszczynski
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T
Section of Macromolecular Analysis, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
Chrambach
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A
D015825:Chromosomes, Fungal / Q000648:ultrastructure*; D004271:DNA, Fungal / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007202:Indicators and Reagents; D008970:Molecular Weight; D012568:Schizosaccharomyces / Q000235:genetics*
D004271:DNA, Fungal; D007202:Indicators and Reagents