Presence and localization of proteins immunologically related to erythrocyte protein 4.1 in human skin.
Analogues of human erythrocyte protein 4.1 have been examined in the human skin by immunochemical techniques using anti-human erythrocyte protein 4.1 antibodies. Immunoblot analysis revealed that human epidermis contains 4.1-like proteins of 80 kDa and 78 kDa that cross react with anti-protein 4.1 antibodies. Analysis with immunofluorescence microscopy revealed that the plasma membrane of the human epidermal keratinocyte was stained intensively in the basal cells, whereas spinous cells were moderately stained. It is noted that eccrine sweat gland cells and ductal cells were also stained in the peripheral cytoplasma. Taken together, these results demonstrate that 4.1-like proteins are present in human epidermal keratinocytes, eccrine sweat gland cells and ductal cells. The present findings enable us to suggest that a membrane skeletal protein lattice might exist in these cells.
Shimizu
T
T
Department of Dermatology, Hokkaido University School of Medicine, Sapporo, Japan.
Takakuwa
Y
Y
Koizumi
H
H
Ishibashi
T
T
Ohkawara
A
A
D000906:Antibodies; D001666:Binding Sites, Antibody; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins; D003599:Cytoskeleton / Q000737:chemistry; D004817:Epidermis / Q000737:chemistry; D006801:Humans; D015151:Immunoblotting; D015603:Keratinocytes / Q000737:chemistry; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D008856:Microscopy, Fluorescence; D009479:Neuropeptides; D012867:Skin / Q000737:chemistry* / Q000648:ultrastructure; D013545:Sweat Glands / Q000737:chemistry* / Q000648:ultrastructure
D000906:Antibodies; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D009479:Neuropeptides; C028841:erythrocyte membrane band 4.1 protein; C120557:erythrocyte membrane protein band 4.1-like 1