Thrombospondin and a 140 kd fragment promote adhesion and neurite outgrowth from embryonic central and peripheral neurons and from PC12 cells.
The ability of thrombospondin (TSP), an extracellular matrix glycoprotein, and two proteolytic fragments to support adhesion and neurite outgrowth from embryonic dorsal root ganglia, spinal cord neurons, and PC12 cells was examined. Anti-TSP antibodies or a synthetic peptide (GRGDS) containing an RGD cell-binding region was also added to cells plated on TSP. TSP and its 140 kd fragment were more efficient than laminin controls in supporting adhesion. Neurites formed on laminin, on varying concentrations of TSP, and particularly the 140 kd fragment. The amino-terminal heparin-binding domain supported little adhesion and outgrowth. Both adhesion and process outgrowth on TSP were inhibited by addition of anti-TSP antibodies, but not GRGDS.
O'Shea
K S
KS
Department of Anatomy and Cell Biology, University of Michigan Medical School Ann Arbor 48109.
Liu
L H
LH
Dixit
V M
VM
D000310:Adrenal Gland Neoplasms / Q000473:pathology* / Q000503:physiopathology / Q000648:ultrastructure; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001369:Axons / Q000187:drug effects / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology* / Q000196:embryology / Q000648:ultrastructure; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000196:embryology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D009842:Oligopeptides / Q000276:immunology / Q000494:pharmacology; D010446:Peptide Fragments / Q000276:immunology / Q000494:pharmacology*; D010525:Peripheral Nerves / Q000166:cytology* / Q000196:embryology / Q000648:ultrastructure; D010673:Pheochromocytoma / Q000473:pathology* / Q000503:physiopathology / Q000648:ultrastructure; D010980:Platelet Membrane Glycoproteins / Q000276:immunology / Q000494:pharmacology*; D051381:Rats; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology / Q000648:ultrastructure; D019699:Thrombospondins; D014407:Tumor Cells, Cultured
D000906:Antibodies; D009842:Oligopeptides; D010446:Peptide Fragments; D010980:Platelet Membrane Glycoproteins; D019699:Thrombospondins; C049554:glycyl-arginyl-glycyl-aspartyl-serine