title: Isolation of a major form of pepsinogen from gastric mucosa of horses.
abstract: In mammalian species studied previously, pepsinogen consisted of biochemically different groups of isozymogens. By use of gel filtration chromatography and electrophoresis, we isolated a predominant pepsinogen from the gastric mucosa of a horse. Peptide mapping with V8 protease revealed differences with its porcine homologue. However, porcine and equine pepsinogens, when activated to pepsin, had a similar pattern of activity when hemoglobin was used as substrate. Those results suggest that differences must exist in the primary structure of the pepsinogens of the 2 species.
authors.affiliation: Department of Anatomy and Physiology, Faculty of Veterinary Medicine, University of Montreal, Quebec, Canada.
authors.forename: G
authors.initials: G
authors.lastname: Khittoo
authors.forename: L
authors.initials: L
authors.lastname: Vermette
authors.forename: G
authors.initials: G
authors.lastname: Nappert
authors.forename: N
authors.initials: N
authors.lastname: Lariviere
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism; D005753:Gastric Mucosa / Q000737:chemistry*; D006454:Hemoglobins / Q000378:metabolism; D006736:Horses / Q000378:metabolism*; D010435:Pepsinogens / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D010449:Peptide Mapping; D013552:Swine
chemical_list: D006454:Hemoglobins; D010435:Pepsinogens; D010450:Endopeptidases