title: 32P-postlabeling analysis of DNA adducts in rat livers after treatment with genotoxic and non-genotoxic 4-aminoazobenzene derivatives.
abstract: Formation of hepatic DNA adducts was studied in rats following intraperitoneal administration of a hepatocarcinogen, 3-methoxy-4-aminoazobenzene (3-MeO-AAB) and a non-hepatocarcinogen, 2-methoxy-4-aminoazobenzene (2-MeO-AAB). The 32P-post-labeling assay revealed 3-MeO-AAB to give more than 20-fold higher amounts of DNA adducts than did 2-MeO-AAB. Furthermore, five adducts, one of which accounted for over 70% of the total modified bases, were found in DNA from 3-MeO-AAB-treated rats, whereas only one adduct was apparent in 2-MeO-AAB-treated DNA. Our data thus suggested that the difference in hepatocarcinogenic activity between 3-MeO-AAB and 2-MeO-AAB might be, at least in part, dependent on quantitative and qualitative differences in their azo dye-DNA adduct formation in the rat liver.
authors.affiliation: Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Kanokoden, Nagoya, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kojima
authors.forename: M
authors.initials: M
authors.lastname: Degawa
authors.forename: Y
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: M
authors.initials: M
authors.lastname: Tada
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010761:Phosphorus Radioisotopes; D051381:Rats; D011916:Rats, Inbred F344; D010128:p-Aminoazobenzene / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D002273:Carcinogens; D010761:Phosphorus Radioisotopes; C053767:2-methoxy-4-aminoazobenzene; D010128:p-Aminoazobenzene; C044436:3-methoxy-4-aminoazobenzene; D004247:DNA