title: A Brassica napus mRNA expressed specifically in developing microspores.
abstract: The I3 cDNA isolated from a library made from 2-4 mm (immature) anthers of Brassica napus shows microspore-specific expression. Homologous transcripts are detected in buds and anthers of male-fertile plants, but not in green tissues, roots, or in cytoplasmic male-sterile buds. High expression of the transcript is limited to microspores entering and undergoing mitosis. The predicted peptide sequence of the cDNA shows an unusual repeated alanine/proline motif at the C-terminus, which may be of importance in the native protein structure.
authors.affiliation: Department of Botany, University of Leicester, U.K.
authors.forename: M R
authors.initials: MR
authors.lastname: Roberts
authors.forename: F
authors.initials: F
authors.lastname: Robson
authors.forename: G D
authors.initials: GD
authors.lastname: Foster
authors.forename: J
authors.initials: J
authors.lastname: Draper
authors.forename: R J
authors.initials: RJ
authors.lastname: Scott
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005718:Gametogenesis; D015870:Gene Expression; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D004247:DNA