title: Analysis of non-heme iron in arachidonate 12-lipoxygenase of porcine leukocytes.
abstract: Arachidonate 12-lipoxygenase of porcine leukocytes, which was purified to homogeneity by immunoaffinity chromatography, was analyzed for iron content by atomic absorption spectrophotometry. The enzyme contained 0.70 +/- 0.09 g atom of iron per mol of enzyme (mean +/- S.D., n = 4). Inorganic iron, which was added to the enzyme solution as an internal standard, was recovered in almost 100% yield. Among various iron chelators tested, only 2,2'-dipyridyl at 1 mM inactivated the enzyme by 87%, but the enzyme was not reactivated by the addition of excess ferrous or ferric iron.
authors.affiliation: Department of Biochemistry, Tokushima University School of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Matsuda
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Yoshimoto
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
authors.forename: A
authors.initials: A
authors.lastname: Miyatake
mesh_terms: D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D007501:Iron / Q000032:analysis*; D007962:Leukocytes / Q000201:enzymology*; D013054:Spectrophotometry, Atomic; D013552:Swine
chemical_list: D007501:Iron; D001092:Arachidonate 12-Lipoxygenase