The nucleotide sequence and luteovirus-like nature of RNA 1 of an aphid non-transmissible strain of pea enation mosaic virus.
An examination of the genomic strategy of pea enation mosaic virus (PEMV) RNA 1 has verified strong organizational and sequence relationships between PEMV and the beet western yellows-potato leafroll luteovirus subgroup. Sequence analysis of RNA 1 demonstrated five predominant open reading frames (ORFs). The extreme 5' ORF encodes a 34K product of unknown function. The second ORF encodes an 84K product which overlaps 90% of ORF 1 (in a unique reading frame) and is expressed by internal initiation beginning at the second start codon from the 5' terminus. This protein contains a protease-like motif characteristic of serine- and cysteine-based proteases, suggesting involvement in post-translational processing of viral translation products. The third ORF is characterized by a number of RNA polymerase motifs and a helicase-like motif typical of RNA-dependent RNA polymerases. It overlaps (out of frame) the ORF 2 product and is proposed to be expressed by a frameshift fusion of the ORF 2 and ORF 3 products. The fourth ORF encodes the viral coat protein, and is immediately followed in frame by a 33K ORF thought to represent the aphid transmission subunit of the PEMV virion. Northern blot analysis of polysome-associated RNA suggests that both products are expressed from an 1800 nucleotide subgenomic mRNA, with the 33K product expressed as a read-through fusion with the coat protein monomer.
Demler
S A
SA
Department of Plant Pathology, University of Wisconsin, Madison 53706.
de Zoeten
G A
GA
D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000382:microbiology; D001483:Base Sequence; D015152:Blotting, Northern; D007887:Fabaceae / Q000382:microbiology; D015870:Gene Expression; D007438:Introns; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D016366:Open Reading Frames; D010946:Plants, Medicinal; D012367:RNA, Viral / Q000502:physiology; D016415:Sequence Alignment; D015678:Viral Structural Proteins / Q000235:genetics
D012367:RNA, Viral; D015678:Viral Structural Proteins