Characterization of monoclonal anti-rabbit apolipoprotein E antibodies and chemical composition of lipoproteins separated by anti-apolipoprotein E immuno-affinity chromatography.
Six mouse monoclonal antibodies against rabbit apolipoprotein E (apo E) have been developed. Of these monoclonal antibodies, clone 5 revealed a high affinity for purified apo E, very low density lipoprotein (VLDL) and beta-VLDL. This monoclonal antibody was used to prepare an immunoaffinity column. Coupled to Sepharose 4B, this antibody allowed complete removal of lipoproteins containing apo E from plasma of New Zealand white (NZW) rabbits; 62, 46, 14, and 3% of VLDL-, IDL-, LDL-, and HDL-protein, respectively, were bound to the anti-apo E affinity column. The bound VLDL was significantly rich in free cholesterol (FC) and cholesteryl esters (CE) relative to the unbound VLDL, whereas bound IDL, LDL and HDL were significantly rich in FC only. All of the bound fractions were characterized by significantly increased ratios of FC/phospholipids (PL). These results indicate that the two lipoprotein populations with and without apo E have different lipid compositions. The relatively high content of cholesterol in lipoproteins containing apo E suggests a contribution of apo E to plasma cholesterol transport.
Yoon
T H
TH
3rd Department of Internal Medicine, University of Tokyo.
Mori
N
N
Kitamura
K
K
Ishibashi
S
S
Shimano
H
H
Mokuno
H
H
Gotoda
T
T
Takaku
F
F
Yamada
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001057:Apolipoproteins E / Q000276:immunology*; D001692:Biological Transport; D002784:Cholesterol / Q000097:blood; D002846:Chromatography, Affinity; D005260:Female; D006493:Heparin; D008074:Lipoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010743:Phospholipids / Q000378:metabolism; D011973:Receptors, LDL / Q000276:immunology
D000911:Antibodies, Monoclonal; D001057:Apolipoproteins E; D008074:Lipoproteins; D010743:Phospholipids; D011973:Receptors, LDL; D006493:Heparin; D002784:Cholesterol