title: Primary structure of bovine cathepsin S. Comparison to cathepsins L, H, B and papain.
abstract: The primary structure of bovine cathepsin S was determined by combining results of protein and peptide sequencing with the sequence deduced from nucleic acid sequencing. Using polymerase chain reaction (PCR) technology, cDNA clones commencing at amino acid 22 of the mature enzyme and continuing through the 3' untranslated region of bovine cathepsin S mRNA were isolated and sequenced. The open reading frame in these overlapping clones correctly predicts the determined amino acid sequence of 13 tryptic peptides derived from purified bovine spleen cathepsin S. The deduced amino acid sequence shows that mature bovine cathepsin S consists of 217 amino acids corresponding to a molecular weight of 23.7 kDa. Cathepsin S belongs to the papain superfamily of lysosomal cysteine proteinases and shares 41% identity with papain. Amino acid sequence identities of bovine cathepsin S to human cathepsins L, H, and B are 56%, 47% and 31% respectively.
authors.affiliation: Institute of Biochemistry, Faculty of Medicine, University of Halle, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Wiederanders
authors.forename: D
authors.initials: D
authors.lastname: Broemme
authors.forename: H
authors.initials: H
authors.lastname: Kirschke
authors.forename: N
authors.initials: N
authors.lastname: Kalkkinen
authors.forename: A
authors.initials: A
authors.lastname: Rinne
authors.forename: T
authors.initials: T
authors.lastname: Paquette
authors.forename: P
authors.initials: P
authors.lastname: Toothman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002401:Cathepsin B / Q000737:chemistry; D056655:Cathepsin H; D056668:Cathepsin L; D002403:Cathepsins / Q000737:chemistry / Q000235:genetics*; D002417:Cattle; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D006801:Humans; D008969:Molecular Sequence Data; D010206:Papain / Q000737:chemistry; D011487:Protein Conformation; D016415:Sequence Alignment
chemical_list: D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; C534281:CTSL protein, human; D056668:Cathepsin L; C534126:CTSH protein, human; D056655:Cathepsin H; D010206:Papain; C028217:cathepsin S