[Effects of cernitin pollen extract (CN-009) on the isolated bladder smooth muscles and the intravesical pressure].
Cernitin pollen extract (CN-009), extract from several pollen species, has been used for urinary dysfunction. As its mode of action has not been clarified, we investigated the action of CN-009 on the isolated bladder smooth muscles of rats, guinea pigs and cats and the intravesical pressure in female rats. CN-009 contracted isolated detrusor muscles of rats, guinea pigs and cats in a concentration-dependent manner. In the guinea pig detrusor muscle, the contractile effect of CN-009 was depressed by atropine, diphenhydramine and increased by cimetidine. In the rat detrusor muscle, the CN-009-induced contraction was depressed by atropine. In adult rats (11-23 weeks old) and aged rats (2 years old), CN-009 showed a dose-dependent increase of intravesical pressure to the same extent in spite of the fact that the aged rats had a lower responsiveness to acetylcholine. In adult rats, the CN-009-induced increase of intravesical pressure was reduced completely by atropine and partly reduced by phentolamine and guanethidine. Three weeks consecutive oral administration of CN-009 tended to increase the basal intravesical pressure and tended to elevate the isoproterenol-induced decrease and serotonin-induced increase in the intravesical pressure. These results suggest that CN-009 contracts the detrusor muscle, a process that is mainly mediated by muscarinic receptor activation. The contraction induced by CN-009 of detrusor muscle causes the increase of intravesical pressure.
Onodera
S
S
Department of Pharmacology, Toho University School of Medicine, Tokyo, Japan.
Yoshinaga
M
M
Takenaga
K
K
Toyoshima
A
A
Uchiyama
T
T
D000375:Aging; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002415:Cats; D002927:Cimetidine / Q000494:pharmacology; D004155:Diphenhydramine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects*; D010936:Plant Extracts; D011058:Pollen; D011312:Pressure; D051381:Rats; D011976:Receptors, Muscarinic / Q000378:metabolism; D012434:Secale; D012701:Serotonin / Q000494:pharmacology; D013045:Species Specificity; D001743:Urinary Bladder / Q000187:drug effects*
D010936:Plant Extracts; D011976:Receptors, Muscarinic; D012701:Serotonin; D001285:Atropine; D002927:Cimetidine; C010070:cernilton; D004155:Diphenhydramine; D007545:Isoproterenol