[The results of using direct solid-phase immunoenzyme analysis for assessing the specific activity of rabies vaccines].
The effectiveness of the solid-phase enzyme immunoassay (EIA) in the determination of the specific activity of rabies vaccines is evaluated in comparison with that of the protective test in mice. Inactivated tissue-culture, concentrated tissue-culture and purified cerebral tissue vaccines for human use were studied. The methods for performing two EIA variants and evaluating the results are described. The average level of correlation between the results of EIA and the protective test for vaccines of different groups was revealed (0.546), the highest correlation index being obtained for tissue-culture vaccines: 0.753. On the basis of the data obtained in this study the expediency of using EIA for the determination of the specific activity of rabies vaccines has been substantiated.
Chernov
S M
SM
Tsetlin
E M
EM
Botvinkin
A D
AD
Romanova
L N
LN
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity / Q000276:immunology*; D005069:Evaluation Studies as Topic; D007124:Immunoenzyme Techniques; D051379:Mice; D009316:National Institutes of Health (U.S.); D011819:Rabies Vaccines / Q000276:immunology*; D011820:Rabies virus / Q000276:immunology*; D014481:United States; D015164:Vaccines, Inactivated / Q000276:immunology
D000914:Antibodies, Viral; D011819:Rabies Vaccines; D015164:Vaccines, Inactivated
Rezul'taty ispol'zovaniia priamogo tverdofaznogo immunofermentnogo analiza dlia otsenki spetsificheskoĭ aktivnosti antirabicheskikh vaktsin.