Requirements for the inactivation of ribosomes by gelonin.
Inactivation of Artemia salina and rabbit ribosomes by gelonin requires ATP and a high-Mr factor present in the rabbit reticulocyte-lysate post-ribosomal supernatant. The kinetic constants of the gelonin-catalysed release of adenine from A. salina ribosomes are Km = 4.35 microM and Kcat. = 0.1 min-1 in the absence of cofactors, and Km = 1.15 microM and Kcat. = 108 min-1 in their presence. The last two values are similar to those measured for ricin A chain in the absence of cofactors (Km = 2.02 microM and Kcat. = 317 min-1).
Sperti
S
S
Dipartimento di Patologia sperimentale dell'Università di Bologna, Italy.
Brigotti
M
M
Zamboni
M
M
Carnicelli
D
D
Montanaro
L
L
D000225:Adenine / Q000378:metabolism; D000818:Animals; D001156:Artemia; D007700:Kinetics; D010940:Plant Proteins / Q000494:pharmacology*; D011072:Poly U / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000187:drug effects* / Q000378:metabolism; D012276:Ricin / Q000494:pharmacology
D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D054789:Ribosome Inactivating Proteins, Type 1; D011072:Poly U; C025674:GEL protein, Gelonium multiflorum; D012276:Ricin; D000225:Adenine