Molecular assignment of a translocation breakpoint in acute myeloid leukemia with t(8;21).
An 8;21 translocation is a common chromosome abnormality associated with acute myeloblastic leukemia with maturation (M2 of French-American-British (FAB) classification). We have isolated chromosome 21 Notl linking clones; pulsed field gel electrophoresis analysis with one clone (LL263) detected an altered fragment of Notl-digested leukemic cell DNA carrying t(8;21). The altered fragment was shown to be produced by the 8;21 translocation. The breakpoint in chromosome 21 was located about 13 kb to 100 kb proximal to the LL263 Notl site. Because the LL263 clone has a CpG island and is a short distance from the breakpoint, the clone itself may be considered as a candidate for part of the t(8;21) associated gene.
Shimizu
K
K
Department of Immunology and Virology, Saitama Cancer Center Research Institute, Japan.
Ichikawa
H
H
Miyoshi
H
H
Ohki
M
M
Kobayashi
H
H
Maseki
N
N
Kaneko
Y
Y
D000208:Acute Disease; D015139:Blotting, Southern; D001853:Bone Marrow / Q000473:pathology; D002460:Cell Line; D002891:Chromosomes, Human, Pair 21; D002898:Chromosomes, Human, Pair 8; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007951:Leukemia, Myeloid / Q000097:blood / Q000235:genetics* / Q000473:pathology; D015183:Restriction Mapping; D014178:Translocation, Genetic
D004273:DNA, Neoplasm