Detection of 14-kDa group II phospholipase A2 in human seminal plasma.
About 90% of phospholipase A2 activity detected in human seminal plasma reacted with monoclonal antibodies raised against human synovial fluid phospholipase A2. The crude seminal plasma yielded a pure immuno-cross-reactive phospholipase A2 preparation in a single purification step using immuno-affinity chromatography. The amino acid sequence of the N-terminal 20 residues of this seminal enzyme was determined and found to be identical with that of human synovial phospholipase A2. Thus, it is suggested that human seminal plasma contains phospholipase A2, belonging to the 14-kDa group II enzyme family, as the major isoenzyme.
Takayama
K
K
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Hara
S
S
Kudo
I
I
Inoue
K
K
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity; D003429:Cross Reactions; D006801:Humans; D015151:Immunoblotting; D007527:Isoenzymes / Q000276:immunology / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010741:Phospholipases A / Q000276:immunology / Q000302:isolation & purification*; D054467:Phospholipases A2; D012661:Semen / Q000201:enzymology*; D012689:Sequence Homology, Nucleic Acid; D013582:Synovial Fluid / Q000201:enzymology
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D010741:Phospholipases A; D054467:Phospholipases A2