Prostaglandin D2 metabolite stimulates collagen synthesis by human osteoblasts during calcification.
We investigated the effect of the prostaglandin D2 metabolite delta 12-PGJ2 (9-Deoxy-delta 9, delta 12-13,14-dihydroprostaglandin 2D) on collagen synthesis in human osteoblasts. delta 12-PGJ2 at 10(-5) M enhanced collagen synthesis in the presence of 2 mM alpha-glycerophosphate-2Na. The stimulative effect appeared as early as 3 days after addition and continued until 22 days. The enhancement of type I collagen synthesis was confirmed by polyacrylamide gel electrophoresis. The potency was the same as 10(-8) M 1 alpha, 25 dihydroxy vitamin D3 (1,25(OH)2D3). Northern blot analysis showed that 10(-5) M delta 12-PGJ2 and 10(-8) M 1,25(OH)2D3 enhanced the transcription of type I procollagen (alpha 1) mRNA levels in osteoblasts.
Tasaki
Y
Y
Department of Pharmacology, Tokyo Metropolitan Institute of Gerontology, Japan.
Takamori
R
R
Koshihara
Y
Y
D015152:Blotting, Northern; D002113:Calcification, Physiologic / Q000187:drug effects*; D002117:Calcitriol / Q000494:pharmacology; D002118:Calcium / Q000032:analysis; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D015232:Dinoprostone / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005994:Glycerophosphates / Q000494:pharmacology; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D010521:Periosteum / Q000166:cytology; D010758:Phosphorus / Q000032:analysis; D015230:Prostaglandin D2 / Q000378:metabolism / Q000494:pharmacology / Q000031:analogs & derivatives
D005994:Glycerophosphates; D010758:Phosphorus; C037112:9-deoxy-delta-9-prostaglandin D2; D003094:Collagen; C029620:alpha-glycerophosphoric acid; D002117:Calcitriol; D015232:Dinoprostone; D015230:Prostaglandin D2; D002118:Calcium