[A method for assessing the microfloral status of the human intestines by the quantity of adhesion-active bacteria and by the type of adhesins].
The method for the evaluation of the state of intestinal microflora in the diagnosis of enteric diseases by the determination of the number of actively adhesive bacteria and the type of adhesins in the primary culture of human microflora has been developed. This method permits the simultaneous analysis of all colonies grown in a given Petri dish by the sign of their adhesion, which eliminates the possibility of mistakes due to the occasional choice of colonies, as it may happen in the agglutination test. The time necessary for the study of adhesive properties is reduced, as the result is evaluated as early as on day 2 after the inoculation of the seed material. It is also of importance that the method is simple in realization, needs no special equipment, preparation and staining of the material. To determine the type of adhesins, a series of identical inoculations may be obtained by replication with the use of red blood cells of different animals. The proposed method may be used for the confirmation of the etiological role of enterobacteria as evidenced by the presence of high percentage of their actively adhesive colonies.
Gizatulina
S S
SS
Birger
M O
MO
Kulinich
L I
LI
Fish
N G
NG
Mazitova
O P
OP
Biriukova
N V
NV
D001419:Bacteria / Q000302:isolation & purification*; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000032:analysis*; D001431:Bacteriological Techniques; D002648:Child; D015169:Colony Count, Microbial; D003967:Diarrhea / Q000382:microbiology; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D006801:Humans; D007422:Intestines / Q000382:microbiology*
D001426:Bacterial Proteins
Sposob otsenki sostoianiia mikroflory kishechnika cheloveka po kolichestvu adgezivno-aktivnykh bakteriĭ i tipu adgezinov.