[Comparative study of minimal inhibitory concentration (MIC) and minimal lethal concentration (MLC) values for tetracycline, monocycline, erythromycin and rokitamycin against eleven strains of Chlamydia trachomatis].
We evaluated the efficacy of tetracycline, minocycline, erythromycin and rokitamycin (rikamycine, TMS-19Q) in controlling in vitro propagation of Chlamydia trachomatis in HeLa 229 cells. Ten recent clinical isolates of Chlamydia trachomatis and one fast-growing strain were tested with inocula of 100-1,000 inclusion forming units per well of a 96-wheel microculture plate. Chlamydia trachomatis inclusions were detected by an immunoperoxidase-antiperoxidase procedure (PAP), including a genus-specific monoclonal antibody. Minimal inhibitory concentration (MIC) geometric means and ranges were respectively 0.128, 0.015-0.25 mg/l tetracycline, 0.001, less than or equal to 0.001-0.004 mg/l minocycline, 0.187, 0.031-0.5 mg/l erythromycin, and 0.005, less than or equal to 0.001-0.062 mg/l rokitamycin; minimal lethal concentration (MLC) geometric means and ranges were 6.79, 0.125-32 mg/l tetracycline, 0.225, 0.062-2 mg/l minocycline, 3.37, 1-32 mg/l erythromycin, and 0.112, 0.031-1 mg/l rokitamycin. Since rokitamycin appears to be the more bactericidal from the four antibiotics tested, clinical studies in sexually transmitted diseases are indicated.
Bianchi
A
A
Laboratoire de Bactériologie-Virologie, Hôpital Saint-Louis, Université Paris VII.
Scieux
C
C
Robache
S
S
Vassias
I
I
Pérol
Y
Y
D002692:Chlamydia trachomatis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004917:Erythromycin / Q000494:pharmacology*; D066298:In Vitro Techniques; D008911:Minocycline / Q000494:pharmacology*; D015644:Miocamycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology*
D015644:Miocamycin; D004917:Erythromycin; D013752:Tetracycline; D008911:Minocycline; C033383:rokitamycin
Etude comparative des concentrations minimales inhibitrices (CMI) et des concentrations minimales léthales (CML) de la tétracycline, de la minocycline, de l'érythromycine et de la rokitamycine sur onze souches de Chlamydia trachomatis.