Sex identification by polymerase chain reaction using X-Y homologous primer.
A method of sex identification using the polymerase chain reaction technique is described. Using a pair of nucleotide primers from an X-Y homologous region, both the X and the Y sequences can be amplified simultaneously, and more importantly, they result in fragments of different lengths. The success of the procedure is therefore monitored by the presence of a X-specific band while sex is identified by the presence or absence of a Y-specific band.
Nakahori
Y
Y
Department of Congenital Abnormalities Research, National Children's Medical Research Center, Tokyo, Japan.
Hamano
K
K
Iwaya
M
M
Nakagome
Y
Y
D001483:Base Sequence; D004277:DNA, Single-Stranded; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D012732:Sex Determination Analysis; D014960:X Chromosome; D014998:Y Chromosome
D004277:DNA, Single-Stranded