Platelet factor 4 mRNA expression in human erythroleukemic cells: regulation by phorbol esters and certain cytokines.
Developing megakaryocytes are distinguished from progenitor cells by the appearance of platelet proteins such as platelet factor 4 (PF 4). The human erythroleukemic cell line HEL can also be induced to produce PF 4 by incubation in phorbol esters. HEL cells were used here as a model system in which to study the phenomenon of inducible PF 4 production at both the mRNA and protein levels. The cytokines interleukin 1 beta (IL-1 beta), interleukin 3 (IL-3), interleukin 6 (IL-6), granulocyte-macrophage colony-stimulating factor (GM-CSF), erythropoietin (EPO), and transforming growth factor-beta (TGF-beta) were also evaluated for their effects on PF 4 mRNA induction in HEL cells.
Hunt
P
P
Laboratory of Stem Cell Biology, Amgen Incorporated, Thousand Oaks, California 91320.
Hokom
M M
MM
Fisher
C A
CA
Jacobsen
F W
FW
Wiemann
B
B
Martin
F H
FH
D000954:Antigens, Surface / Q000032:analysis; D001792:Blood Platelets / Q000276:immunology; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D016207:Cytokines / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics*; D010978:Platelet Factor 4 / Q000235:genetics* / Q000378:metabolism; D010980:Platelet Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D000954:Antigens, Surface; D016207:Cytokines; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D016212:Transforming Growth Factor beta; D010978:Platelet Factor 4; D004247:DNA; D003513:Cycloheximide; D013755:Tetradecanoylphorbol Acetate