Effect on N1,N14-bis-(ethyl)-homospermine (BE-4-4-4) on the growth of U-251 MG and SF-188 human brain tumor cells.
We studied the effects of the spermine analogue N1,N14-bis-(ethyl)-homospermine (BE-4-4-4) on growth, survival and polyamine levels in cultured U-251 MG and SF-188 human brain tumor cells. After 48 hr of treatment at concentrations of 1 microM or higher, BE-4-4-4 accumulated in cells with a concomitant decrease in intracellular putrescine, spermidine and spermine concentrations. Growth inhibition by 10 microM BE-4-4-4 began at 6 hr and peaked between 16 and 24 hr. The analogue was also increasingly cytotoxic with doses between 1 and 10 microM and with treatment times between 16 and 48 hr. Polyamines added 1 day after BE-4-4-4 lowered the intracellular concentrations of the analogue but did not reverse its growth-inhibitory activity. When added simultaneously with the analogue, however, polyamines caused a decrease in analogue concentration that was accompanied by a block to the growth inhibition. BE-4-4-4 has a higher affinity for DNA than spermine has, but is less able to aggregate DNA. Its growth-inhibitory and cytotoxic effects support our hypothesis that polyamine analogues that enter cells and replace natural polyamines at DNA binding sites, without fulfilling their biologic functions, should act as antiproliferative agents.
Basu
H S
HS
Department of Neurological Surgery, School of Medicine, University of California, San Francisco 94143.
Pellarin
M
M
Feuerstein
B G
BG
Deen
D F
DF
Marton
L J
LJ
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001932:Brain Neoplasms; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism / Q000494:pharmacology; D013095:Spermidine / Q000378:metabolism / Q000494:pharmacology; D013096:Spermine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology
D000970:Antineoplastic Agents; D011073:Polyamines; C058306:N(1),N(14)-bis(ethyl)homospermine; D013096:Spermine; D013095:Spermidine; D011700:Putrescine