Demonstration of calcium in dermal melanocytes of Xenopus laevis and Poecilia reticulata with electron energy-loss spectroscopy and electron spectroscopic imaging.
The subcellular distribution of calcium in dermal melanocytes of Xenopus laevis and Poecilia reticulata has been analysed. Using two cytochemical methods, phosphate precipitation and a combined oxalate-pyroantimonate technique, electron energy-loss spectroscopy and electron spectroscopic imaging have been applied for elemental analysis. Both precipitation techniques revealed a high calcium content in the melanosomes of both species. Calcium was also located in the vicinity of collagen fibrils and in the plasma membrane.
Müller
T
T
Cinematic Cell Research Group, J. W. Goethe-University, Frankfurt, Germany.
Bereiter-Hahn
J
J
D000818:Animals; D000965:Antimony; D002118:Calcium / Q000032:analysis*; D011232:Chemical Precipitation; D006651:Histocytochemistry; D007091:Image Processing, Computer-Assisted; D008544:Melanocytes / Q000737:chemistry* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010070:Oxalates; D010710:Phosphates; D011036:Poecilia; D012867:Skin / Q000737:chemistry*; D013057:Spectrum Analysis; D014982:Xenopus laevis
D010070:Oxalates; D010710:Phosphates; C009002:potassium antimonate; D000965:Antimony; D002118:Calcium