title: Primary structure of two forms of gonadotropin-releasing hormone from brains of the American alligator (Alligator mississippiensis).
abstract: Two forms of gonadotropin-releasing hormone (GnRH) have been purified from brains of the American alligator, Alligator mississippiensis, using reverse-phase high-pressure liquid chromatography (HPLC). The concentration of total GnRH was 8.8 ng/g of frozen brain tissue or 21.1 ng per brain. The amino acid sequence of each form of GnRH was determined using automated Edman degradation. The presence of the N-terminal pGlu residue was established by digestion studies with bovine pyroglutamyl aminopeptidase and coelution with synthetic forms of the native peptide. The primary structure of alligator GnRH I is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Gln-Pro-Gly-NH2 and alligator GnRH II is pGlu-His-Trp-Ser-His-Gly-Trp-Tyr-Pro-Gly-NH2.
authors.affiliation: Biology Department, University of Victoria, B.C., Canada.
authors.forename: D A
authors.initials: DA
authors.lastname: Lovejoy
authors.forename: W H
authors.initials: WH
authors.lastname: Fischer
authors.forename: D B
authors.initials: DB
authors.lastname: Parker
authors.forename: J E
authors.initials: JE
authors.lastname: McRory
authors.forename: M
authors.initials: M
authors.lastname: Park
authors.forename: V
authors.initials: V
authors.lastname: Lance
authors.forename: P
authors.initials: P
authors.lastname: Swanson
authors.forename: J E
authors.initials: JE
authors.lastname: Rivier
authors.forename: N M
authors.initials: NM
authors.lastname: Sherwood
mesh_terms: D000489:Alligators and Crocodiles; D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002645:Chickens; D002845:Chromatography; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry / Q000302:isolation & purification*; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry
chemical_list: D010446:Peptide Fragments; D007987:Gonadotropin-Releasing Hormone