Substratum-growth factor collaborations are required for the mitogenic activities of activin and FGF on embryonal carcinoma cells.
When P19 mouse embryonal carcinoma cells are grown in a serum-free N2 medium on surfaces of tissue culture plastic, they die within two days. The death of these P19 cells is prevented by activin A and basic FGF (bFGF). The cells do not divide under these conditions. However, when P19 cells are cultured on substrata of extracellular matrix proteins such as laminin and fibronectin, activin A and bFGF are potent mitogens. These data show that the substratum to which cells are exposed can regulate their mitogenic response to growth factors.
Schubert
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Salk Institute, San Diego, California 92186-5800.
Kimura
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D028341:Activins; D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D003094:Collagen / Q000494:pharmacology*; D003470:Culture Media; D005109:Extracellular Matrix / Q000502:physiology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005353:Fibronectins / Q000494:pharmacology*; D007265:Inhibins / Q000494:pharmacology*; D007700:Kinetics; D007797:Laminin / Q000494:pharmacology*; D008934:Mitogens; D013724:Teratoma
D003470:Culture Media; D005353:Fibronectins; D007797:Laminin; D008934:Mitogens; D016222:Fibroblast Growth Factor 2; D028341:Activins; D007265:Inhibins; D003094:Collagen