title: The effect of dietary zinc deficiency on the lipid composition of the rat erythrocyte membrane.
abstract: The effect of dietary zinc deficiency in the rat on the lipid composition of the erythrocyte membrane was determined. Weanling male Wistar rats were fed an egg white-based diet containing less than 1.0 mg Zn/kg diet ad libitum. Control rats were either pair-fed or ad libitum-fed the basal diet supplemented with 100 mg Zn/kg diet. A zinc refed group was fed the -Zn diet until day 18 and then pair-fed the +Zn diet until day 21. The voluntary feed restriction associated with dietary zinc deficiency resulted in erythrocyte membranes that had depressed phospholipid/protein and elevated cholesterol/phospholipid ratios. Similarly, all feed restricted groups had elevated 22-carbon n-3 polyunsaturated fatty acids (PUFA) and depressed 22-carbon n-6 PUFA concentrations in alkenyl-acyl and diacyl glycerophosphoethanolamine, phosphatidylserine and phosphatidylcholine; they also had depressed 24:2n-6 levels in sphingomyelin. The relative concentrations of phospholipids in the membrane was similar between -Zn and +Zn (ad libitum) groups; however, the -Zn group had significantly less phosphatidyl-serine relative to +Zn (pair-fed) controls.
authors.affiliation: Department of Nutritional Sciences, College of Biological Science, University of Guelph, Ontario, Canada.
authors.forename: E R
authors.initials: ER
authors.lastname: Driscoll
authors.forename: W J
authors.initials: WJ
authors.lastname: Bettger
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000097:blood; D004032:Diet; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005227:Fatty Acids / Q000097:blood; D005229:Fatty Acids, Monounsaturated / Q000097:blood; D005231:Fatty Acids, Unsaturated / Q000097:blood; D005508:Food Deprivation; D008297:Male; D008563:Membrane Lipids / Q000097:blood*; D008565:Membrane Proteins / Q000097:blood; D010713:Phosphatidylcholines / Q000097:blood; D010714:Phosphatidylethanolamines / Q000097:blood; D010718:Phosphatidylserines / Q000097:blood; D010743:Phospholipids / Q000097:blood; D051381:Rats; D011919:Rats, Inbred Strains; D013109:Sphingomyelins / Q000097:blood; D015032:Zinc / Q000172:deficiency*
chemical_list: D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; D008563:Membrane Lipids; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D010743:Phospholipids; D013109:Sphingomyelins; C002449:glycerophosphoethanolamine; D002784:Cholesterol; D015032:Zinc