[A double-labeling study of serotonin neurons that project to the visual cortex of developing rat brain].
Immunohistochemical studies of developing rat brains revealed aggregation of serotonin terminal fibers in the visual cortex at from 7 to 14 postnatal days. This aggregation is transient, disappearing 2 weeks after birth. The cells of origin of these serotonin terminals were investigated using double-labeling with retrogradely transported WGA-apoHRP-Au and serotonin immunohistochemistry. WGA-apoHRP-Au was injected into the rat visual cortex on the 9th day after birth. The rats were allowed to survive for 2 days and sacrificed with perfusion. Cryostat sections of the brain were processed with silver-enhancement to develop the retrogradely transported WGA-apoHRP-Au, and then serotonin immunohistochemistry was performed. Double-labeled cells that were labeled with WGA-apoHRP-Au and serotonin-immunoreactive were found mostly in the raphe medianus and raphe dorsalis in rostral and caudal sections of the mesencephalon, respectively.
Koh
T
T
Department of Ophthalmology, Shiga University of Medical Science, Ohtsu, Japan.
Maeda
T
T
Nakazawa
M
M
Kani
K
K
D000818:Animals; D001921:Brain / Q000254:growth & development*; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D009474:Neurons / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*; D014793:Visual Cortex / Q000166:cytology / Q000378:metabolism*; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins
D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins; C062844:wheat germ agglutinin-apohorseradish peroxidase conjugate gold complex; D012701:Serotonin; D006735:Horseradish Peroxidase