Gene cloning and nucleotide sequence of SM22 alpha from the chicken gizzard smooth muscle.
A full length of cDNA encoding SM22 alpha from chicken gizzard smooth muscle was cloned and sequenced. Cloned cDNA had a total length of 1214bp and contained a single open reading frame which encodes 200 amino acids with a calculated molecular weight 22214. The predicted amino acid sequence was in complete agreement with the sequence determined by Pearlstone et al. using Edman degradation method (J. Biol. Chem. 262:5985, 1987) except for two additional residues, isoleucine and serine at the C-terminus.
Nishida
W
W
Second Department of Internal Medicine, Ehime University School of Medicine, Japan.
Kitami
Y
Y
Abe
M
M
Hiwada
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D005895:Gizzard, Avian / Q000378:metabolism; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D009130:Muscle, Smooth / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics
D008840:Microfilament Proteins; D009124:Muscle Proteins; C058992:Tagln protein, mouse; C051615:transgelin