Tandem arrangement of tRNA(Asp)-encoding genes in Phytophthora spp.
We have cloned a region of repetitive DNA from the phytopathogenic fungus, Phytophthora parasitica. The cloned region consists of 17 highly homologous units arranged in tandem. The consensus sequence is 562 bp long and carries the information for a tRNA(Asp). All sequence motifs required for efficient RNA polymerase III transcription are present, and the tRNA derived from the nucleotide sequence is able to form a complete cloverleaf structure with high homology to previously characterized tRNA(Asp) molecules. The isolated tRNA(Asp) gene cluster is located at a distance of 20 kb from the TRP1 gene of P. parasitica. It comprises about 0.1% of the total genomic DNA. Similar clusters were detected in four other Phytophthora species.
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Gesellschaft für Strahlen- und Umweltforschung mbH München, Abteilung für Molekulare Genetik, Göttingen, F.R.G.
Karlovsky
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D001483:Base Sequence; D016384:Consensus Sequence; D004271:DNA, Fungal / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010838:Phytophthora / Q000235:genetics*; D012320:RNA Polymerase III / Q000378:metabolism; D012331:RNA, Fungal / Q000235:genetics; D012349:RNA, Transfer, Asp / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000502:physiology
D004271:DNA, Fungal; D012331:RNA, Fungal; D012349:RNA, Transfer, Asp; D012320:RNA Polymerase III