Isolation and characterization of an immunogen from the louse Polyplax spinulosa.
The reaction of Polyplax spinulosa protein components with antigen-specific rat antisera and with five plant lectins is described. In immunoblotting, the antisera from rats infested with the louse P. spinulosa specifically recognized from 7 to 11 antigenic components of louse whole-body homogenates. Affinoblotting analysis with lectins showed that 10 of these components were glycoproteins with different types of glycan structure. Only one immunogenic antigen was isolated using immunoaffinity chromatography with the gammaglobulin fraction of an antigen-specific antiserum as immunospecific ligand. Further electrophoretic and immunochemical analyses revealed that it was a glycoprotein with relative molecular weight (RMW) 31 kDa and with high mannose type of oligosaccharide.
Volf
P
P
Department of Parasitology and Hydrobiology, Faculty of Sciences, Charles University, Prague, Czechoslovakia.
Grubhoffer
L
L
D000818:Animals; D000941:Antigens / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis*; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D015151:Immunoblotting; D008003:Phthiraptera / Q000276:immunology*; D051381:Rats; D011918:Rats, Inbred SHR; D011919:Rats, Inbred Strains
D000941:Antigens; D006023:Glycoproteins; D000949:Histocompatibility Antigens Class II