[The effect of the bacterial genome on the expression and behavior of the plasmid determining the Ca2+-dependence of the plague pathogen].
The conjugative cointegrate containing the 47 Md plasmid of Yersinia pestis has been transferred into the strains of the different Yersinia (Yersinia pestis, Yersinia pseudotuberculosis, Yersinia enterocolitica) and Escherichia coli CA. There appeared in the populations of recombinant Yersinia under the conditions of Ca2+ deficit at 37 degrees C the cells coming into the stasis stage or dying. It was shown on the model of Yersinia enterocolitica that bacterial lethality might be prevented by exclusion of the sheep blood from Ca2+ deficient medium. Ca(2+)-dependence was not expressed in Escherichia coli cells in which the cointegrates were prone to deletions although the cad-genes were preserved intact. The latter conclusion is based on the positive reciprocal transfer of the Cad(+)-marker into Yersinia pestis cells.
Kuznetsova
L S
LS
Lebedeva
S A
SA
Grebtsova
N N
NN
Cherniavskaia
A S
AS
Zarenkov
M I
MI
D002118:Calcium / Q000378:metabolism*; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D010957:Plasmids; D014162:Transfection; D015008:Yersinia enterocolitica / Q000235:genetics; D015010:Yersinia pestis / Q000235:genetics* / Q000378:metabolism; D015011:Yersinia pseudotuberculosis / Q000235:genetics
D002118:Calcium
Vliianie genoma bakterii na ékspressiiu i povedenie plazmidy, opredeliaiiushcheĭ Ca2+-zavisimost' chumnogo mikroba.