Effects of mercury and lead on rubidium uptake and efflux in cultured rat astrocytes.
Astrocytes readily sequester lead and mercury (8, 10, 19, 22). Accordingly, studies were undertaken to assess the effects of lead and mercury on homeostatic functions in neonatal rat brain primary astrocyte cultures. Both inorganic and organic mercury, but not lead, significantly inhibited the initial rate (5 min) of uptake of 86RbCl, used as a tracer for K+, at concentrations of 10-100 microM. Mercury and to a lesser extent lead also stimulated the efflux of intracellular 86Rb+ at 10-500 microM. These observations suggest that the astrocyte plasma membrane may be an important target for lead and mercury, and that relatively low concentrations of these heavy metals should inhibit the ability of astrocytes to maintain a transmembrane K+ gradient.
Aschner
M
M
Department of Pharmacology and Toxicology, Albany Medical College, NY 12208.
Chen
R
R
Kimelberg
H K
HK
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism*; D007700:Kinetics; D007854:Lead / Q000494:pharmacology*; D008627:Mercuric Chloride / Q000494:pharmacology*; D008767:Methylmercury Compounds / Q000494:pharmacology*; D051381:Rats; D012413:Rubidium / Q000378:metabolism*
D002712:Chlorides; D008767:Methylmercury Compounds; D007854:Lead; C029891:lead chloride; D008627:Mercuric Chloride; D012413:Rubidium; C032710:rubidium chloride