title: Variability of penicillin-binding proteins from penicillin-sensitive Streptococcus pneumoniae.
abstract: Penicillin-binding proteins (PBPs) from penicillin-susceptible strains of Streptococcus pneumoniae are believed to be fairly similar in contrast to PBPs occurring in resistant isolates. The antigenic variation of PBPs 1a and 2b in 65 penicillin-susceptible strains from different geographic areas and a wide variety of isolation sites was analyzed using a set of specific antisera and monoclonal antibodies. Three strains contained different antigenic variants of PBP 1a, and 50 strains contained one of three antigenic variants found in PBP 2b. Seven patterns of antibody reactivity could be defined; all but one were distinct from those found recently in resistant strains. In addition, electrophoretic mobilities of all six PBPs, compared after conventional SDS-PAGE and fluorography, revealed an unexpected variation of PBP-profiles even for strains of one sero-group. Few strains appeared identical to each other or to the laboratory reference strain R6.
authors.affiliation: Max-Planck Institut für Molekulare Genetik, Berlin, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Hakenbeck
authors.forename: T
authors.initials: T
authors.lastname: Briese
authors.forename: L
authors.initials: L
authors.lastname: Chalkley
authors.forename: H
authors.initials: H
authors.lastname: Ellerbrok
authors.forename: R
authors.initials: R
authors.lastname: Kalliokoski
authors.forename: C
authors.initials: C
authors.lastname: Latorre
authors.forename: M
authors.initials: M
authors.lastname: Leinonen
authors.forename: C
authors.initials: C
authors.lastname: Martin
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000940:Antigenic Variation; D000942:Antigens, Bacterial / Q000032:analysis; D001426:Bacterial Proteins; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006602:Hexosyltransferases; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D002267:Muramoylpentapeptide Carboxypeptidase / Q000032:analysis* / Q000276:immunology; D010403:Penicillin Resistance; D046915:Penicillin-Binding Proteins; D010406:Penicillins / Q000494:pharmacology*; D010458:Peptidyl Transferases; D013296:Streptococcus pneumoniae / Q000145:classification / Q000187:drug effects* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D007106:Immune Sera; D046915:Penicillin-Binding Proteins; D010406:Penicillins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase