Identification of the coat protein gene of bean golden mosaic geminivirus.
An open-reading frame designated 1R1, on DNA component 1 of bean golden mosaic geminivirus (BGMV), has been identified as the coat protein gene. A DraI restriction fragment of BGMV DNA1 that includes 1R1 was inserted into the SmaI pKK223-3 expression vector. The 32 kD protein expressed in Escherichia coli cells reacted with antibodies to the BGMV capsid polypeptide and behaved identically to purified capsid protein in western blots.
Murayama
A
A
NODAI Research Institute, Tokyo University of Agriculture, Japan.
Yamaya
Y
Y
Ikegami
M
M
D000818:Animals; D002213:Capsid / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D015151:Immunoblotting; D009029:Mosaic Viruses / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D011817:Rabbits; D014162:Transfection
D004279:DNA, Viral