title: Enhancement of in vivo and in vitro T cell response against measles virus haemagglutinin after its incorporation into liposomes: effect of the phospholipid composition.
abstract: Artificial phospholipid bilayer vesicles were tested for their capacity to enhance the priming and the restimulation of mouse T cells against the haemagglutinin (H) glycoprotein of the measles virus in vivo and in vitro. H glycoprotein was purified and incorporated into liposomes made of cholesterol, dicetylphosphate and dilauroylphosphatidylcholine (DLPC) or distearoylphosphatidylcholine (DSPC). H in DLPC or DSPC-liposomes was found to be a potent in vivo stimulator of lymph node T cells harvested from mice immunized with measles virus, whereas H glycoprotein in free form did not elicit any proliferative T cell response. When used to immunize naive mice, only H in DSPC-liposomes was able to prime T cells as evidenced by the capacity of lymph node cells to proliferate in the presence of H in liposomes or measles virus as secondary stimulating agents in vitro. H-specific T cell clones derived from animals immunized with H in DSPC-liposomes were able to recognize H glycoprotein both in free form and incorporated into liposomes in the presence of naive spleen cells as APC. However, compared with the liposome forms, 20-fold more H protein in free form was required to elicit a T cell clone response at a similar level. This liposome immune enhancing effect on the T cell clone recognition of H glycoprotein was also observed when peritoneal exudate cells were used as APC. These data demonstrate that the insertion of a membrane-derived antigen into artificial membranes may be a prerequisite for the priming and stimulation of specific T cells both in vivo and in vitro. In addition, the nature of the phospholipid used to build the liposomes appears to be a critical parameter.
authors.affiliation: INSERM U218, Centre Léon Bérard, Lyon, France.
authors.forename: F
authors.initials: F
authors.lastname: Garnier
authors.forename: F
authors.initials: F
authors.lastname: Forquet
authors.forename: P
authors.initials: P
authors.lastname: Bertolino
authors.forename: D
authors.initials: D
authors.lastname: Gerlier
mesh_terms: D000818:Animals; D004337:Drug Carriers; D006389:Hemagglutinins, Viral / Q000008:administration & dosage / Q000276:immunology*; D066298:In Vitro Techniques; D008081:Liposomes; D008213:Lymphocyte Activation; D008458:Measles Vaccine / Q000008:administration & dosage; D008459:Measles virus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010743:Phospholipids / Q000276:immunology; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D004337:Drug Carriers; D006389:Hemagglutinins, Viral; D008081:Liposomes; D008458:Measles Vaccine; D010743:Phospholipids