Human complement component factor B rescues HIV-1-infected leukemic T cells from cytopathic death.
The addition of 5-10% of fresh normal human serum (NHS) from normal individuals into a culture of primarily HIV-1-infected CD4+ leukemic T-cell lines CEM and MT4 was found to rescue the infected cells from cytopathic death, enabling the cells to achieve growth within 10 days. The HIV-1-infected cells cultured in ordinary medium with fetal calf serum (FCS) all died within 10 days. The effect of NHS was ascribed to human complement component factor B and one or more factor B-dependent heat-labile co-factors. The cells which survived in the presence of NHS rapidly lost surface expression of CD4 and became completely resistant to rechallenge by HIV-1. Viral genomes were dramatically reduced in surviving cells within 30 days, and one cell-line CEM completely expelled them during this period. The results suggest that factor B has protective and potential therapeutic significance in HIV-1 infection.
Nozaki-Renard
J
J
Department of Microbiology, Tokyo Medical College, Japan.
Kim
T
T
Iino
T
T
Tsukamoto
T
T
Furutani-Seiki
M
M
Tada
T
T
D015704:CD4 Antigens / Q000378:metabolism; D011415:Complement Factor B / Q000276:immunology*; D003588:Cytopathogenic Effect, Viral; D015658:HIV Infections / Q000276:immunology* / Q000628:therapy; D015497:HIV-1; D006801:Humans; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000276:immunology
D015704:CD4 Antigens; D011415:Complement Factor B