Opposite effect of cytochalasin B on agonist-induced respiratory burst in neutrophils and monocytes.
The effects of cytochalasin B on the respiratory burst, calcium transients and cell shape change of neutrophils and monocytes has been studied. Cytochalasin B enhanced fMLP-induced respiratory burst in neutrophils whereas the opposite effect, i.e. an inhibition close to 50%, was elicited in fMLP-stimulated monocytes. The differences did not depend on calcium homeostasis. On the basis of cell shape changes, and the well known effect of cytochalasin B on actin polymerization, the opposite effects of cytochalasin B could stem from differences between both cell types concerning the pattern of cytoskeleton-membrane interaction which could affect the recruitment and assembly of membrane-bound or cytosolic components of NADPH-oxidase.
Lucas
M
M
Dpto. de Bioquímica, Hospital Universitario V. Macarena, Facultad de Medicina, Sevilla, Spain.
Solano
F
F
D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003571:Cytochalasin B / Q000494:pharmacology*; D003599:Cytoskeleton / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D019255:NADPH Oxidases; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism
D003571:Cytochalasin B; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D010100:Oxygen; D002118:Calcium