Simultaneous high-performance liquid chromatographic determination of ascorbic acid and dehydroascorbic acid in biological samples.
The ascorbic acid (AA)-dehydroascorbic acid redox couple is an important component of many biological systems, and various physiological roles have been described for this vitamin. Simultaneous measurement of both AA and dehydroascorbate using high-performance liquid chromatography (HPLC) has proven difficult owing to detection problems. A simple, single-step HPLC assay for the simultaneous detection of both AA and dehydroascorbate was developed without the burden of derivatization of either compounds. This has proven to be a reliable technique and should be applicable to a wide variety of biological samples.
Cammack
J
J
Department of Pharmacology, School of Pharmacy, University of Kansas, Lawrence 66045.
Oke
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A
Adams
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D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis*; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003683:Dehydroascorbic Acid / Q000032:analysis*; D006801:Humans
D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid