Determination of urinary 2,5-hexanedione concentration by an improved analytical method as an index of exposure to n-hexane.
2,5-Hexanedione is a main metabolite of n-hexane and is considered as the cause of n-hexane polyneuropathy. Therefore, it is useful to measure 2,5-hexanedione for biological monitoring of exposure to n-hexane. The analytical methods existing for n-hexane metabolites, however, were controversial and not established enough. Hence, a simple and precise method for determination of urinary 2,5-hexanedione has been developed. Five ml of urine was acidified to pH 0.5 with concentrated hydrochloric acid and heated for 30 minutes at 90-100 degrees C. After cooling in water, sodium chloride and dichloromethane containing internal standard were added. The sample was shaken and centrifuged. 2,5-Hexanedione concentration in an aliquot of dichloromethane extract was quantified by gas chromatography using a widebore column (DB-1701). Urinary concentration of 2,5-hexanedione showed a good correlation with exposure to n-hexane (n = 50, r = 0.973, p less than 0.001). This method is simple and precise for analysis of urinary 2,5-hexanedione as an index of exposure to n-hexane.
Saito
I
I
Aichi Prefectural Institute of Public Health, Nagoya, Japan.
Shibata
E
E
Huang
J
J
Hisanaga
N
N
Ono
Y
Y
Takeuchi
Y
Y
D000269:Adhesives; D000818:Animals; D001336:Automobiles; D002849:Chromatography, Gas; D004355:Drug Stability; D005260:Female; D006586:Hexanes / Q000032:analysis*; D006588:Hexanones / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007221:Industry; D008297:Male; D016273:Occupational Exposure; D051381:Rats; D013696:Temperature; D013997:Time Factors
D000269:Adhesives; D006586:Hexanes; D006588:Hexanones; C026385:n-hexane; C011269:2,5-hexanedione