Transient expression of beta-glucuronidase in Arabidopsis thaliana leaves and roots and Brassica napus stems using a pneumatic particle gun.
Successful transient expression of beta-glucuronidase (GUS) in Arabidopsis thaliana leaves and roots and Brassica napus stems was obtained after gene delivery with a pneumatic particle gun driven by compressed air. Effects of the pneumatic pressure used to accelerate the particles (accelerating pressure; 85 to 200 kg/cm2) and of preculture periods of plant tissues (0 to 6 days) on the efficiency of gene delivery were studied. In A. thaliana leaves, best results were obtained at 115 kg/cm2 of accelerating pressure and 3 days of preculture. In A. thaliana roots, the optimum was at 200 kg/cm2 of accelerating pressure and 3 days of preculture. These results indicate that both preculture period and accelerating pressure are vital factors that determine the efficiency of gene delivery by particle gun.
Seki
M
M
Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, Japan.
Komeda
Y
Y
Iida
A
A
Yamada
Y
Y
Morikawa
H
H
D001274:Atmospheric Pressure; D001937:Brassica / Q000235:genetics*; D015870:Gene Expression; D005966:Glucuronidase / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D011994:Recombinant Proteins / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
D011994:Recombinant Proteins; D005966:Glucuronidase