Apoptosis of murine BW 5147 thymoma cells induced by dexamethasone and gamma-irradiation.
The mode and the kinetics of the death of T-thymoma cells upon dexamethasone treatment and gamma-irradiation (10Gy) have been studied using flow cytometry and biochemical analysis. It has been shown that the hormone and gamma-irradiation induce cell death by apoptosis. In both cases the cells are initially blocked in G2/M and die only after overcoming the blockage and cytokinesis. A short exposure to dexamethasone results in a cytostatic effect, whereas a cytotoxic effect is absent. Reducing serum concentration to 2% causes more rapid death both following gamma-irradiation and dexamethasone. These results are discussed in relation to cell death and proliferation.
Kruman
I I
II
Institute of Biological Physics, USSR Academy of Sciences, Moscow Region.
Matylevich
N P
NP
Beletsky
I P
IP
Afanasyev
V N
VN
Umansky
S R
SR
D000818:Animals; D001769:Blood; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D003470:Culture Media; D004273:DNA, Neoplasm / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D016195:G2 Phase / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays; D008938:Mitosis / Q000187:drug effects / Q000528:radiation effects; D013945:Thymoma / Q000473:pathology*; D014407:Tumor Cells, Cultured
D003470:Culture Media; D004273:DNA, Neoplasm; D003907:Dexamethasone