Platelet activating factor acetylhydrolase activity in the urine of patients with renal disease.
Platelet activating factor has been demonstrated in blood and urine and has a broad range of effects on kidney function. The kidney possesses the enzymes responsible for PAF synthesis as well as the specific acetylhydrolase which deactivates PAF. We used a radioactive assay to measure PAF-acetylhydrolase activity in urine samples obtained from normal individuals and patients with various nephropathies. Activity was detected in the majority of normal urines with a mean + 2 SD = 0.70 nmol/30 min/ml. Activity exceeding this value was measured in the urines of 11 of 14 patients with diabetes mellitus, 14 of 22 with glomerulonephritis, and 5 of 16 with hypertensive renal disease. Further study is necessary to relate urine hydrolase activity to specific functional and structural abnormalities of the kidney.
Kirschbaum
B
B
Department of Medicine, Medical College of Virginia, Richmond.
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000328:Adult; D003928:Diabetic Nephropathies / Q000201:enzymology; D005260:Female; D005921:Glomerulonephritis / Q000201:enzymology; D006801:Humans; D006977:Hypertension, Renal / Q000201:enzymology; D007674:Kidney Diseases / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D010741:Phospholipases A / Q000652:urine*; D012016:Reference Values
D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase