Ba2+ current oscillation evoked by bradykinin in ras-transformed fibroblasts.
By voltage-clamp recording, we show a novel inward current which oscillates after activation with bradykinin or serum in v-Ki-ras-transformed NIH/3T3 cells. The current oscillation was infrequently observed in control NIH/3T3 fibroblasts. The same stimulation evokes Ca2+ oscillations in the ras-transformed cells but not in parental cells (Fu et al., FEBS Lett. 281, 263-266, 1991). The results suggest that the oscillatory currents are generated by influxes of divalent cations to maintain Ca2+ oscillations in ras-transformed NIH/3T3 cells.
Higashida
H
H
Department of Biophysics, Kanazawa University School of Medicine, Japan.
Hoshi
N
N
Hashii
M
M
Fu
T
T
Noda
M
M
Nozawa
Y
Y
D000818:Animals; D001464:Barium / Q000378:metabolism*; D001920:Bradykinin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D011905:Genes, ras; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D009532:Nickel / Q000494:pharmacology
D001464:Barium; D009532:Nickel; D001920:Bradykinin; D002118:Calcium