title: Attachment of boar sperm to mucosal explants of oviduct in vitro: possible role in formation of a sperm reservoir.
abstract: Ejaculated boar sperm were incubated with explants of porcine oviductal mucosa that had been dissected from the isthmic and ampullar regions of gilts. Sperm bound within minutes to the epithelial surfaces of the explants. Binding was not affected by region (isthmus or ampulla) nor day of estrous cycle (Day 0 or Day 10), but was increased by addition of 70 pg/ml 17 beta-estradiol to the medium. Scanning electron micrographs indicated that sperm bound, via the acrosomal region, to ciliated cells. After 24 h, the numbers of bound sperm dropped significantly, but the motility of the bound sperm did not. A mucous material that entrapped sperm was observed on the epithelial surfaces of 23/32 isthmic and only 4/32 ampullar explants. These results indicate that sperm sticking to ciliated cells and mucus can create a sperm reservoir in the isthmus, but the means by which sperm are released remain unknown.
authors.affiliation: Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville 32610.
authors.forename: S
authors.initials: S
authors.lastname: Suarez
authors.forename: K
authors.initials: K
authors.lastname: Redfern
authors.forename: P
authors.initials: P
authors.lastname: Raynor
authors.forename: F
authors.initials: F
authors.lastname: Martin
authors.forename: D M
authors.initials: DM
authors.lastname: Phillips
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002923:Cilia / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D008855:Microscopy, Electron, Scanning; D009092:Mucous Membrane / Q000502:physiology / Q000648:ultrastructure; D010057:Oviducts / Q000502:physiology* / Q000648:ultrastructure; D013094:Spermatozoa / Q000502:physiology* / Q000648:ultrastructure; D013552:Swine; D013997:Time Factors