[Anti-antibody responses of rabbit to mouse monoclonal antibody against schistosome "target antigen"].
The supernatant of protective McAb (isotype IgG2a) secreted by 1E2 hybridoma line was purified by protein A-sepharose CL-4B column and rabbits were immunized with purified 1E2 McAb. Dot-ELISA, immunodiffusion assay, Western blotting and ELISA were used to observe the presence of anti-McAb and anti-anti-McAb. The main results are: 1. anti-McAb antibody could be detected as early as 20 days post immunization in the immune sera and would be decreased gradually without boosting; otherwise, it would be increased quickly and the titers of anti-McAb were increased within 5 days post-boosting and could be maintained at a plateau for 30-50 days, and then decreased gradually to the normal; 2. there were different anti-anti-McAbs in the immune sera. Anti-anti-McAb, like anti-anti-idiotype antibody, can recognize different schistosome antigens and 90, 68 or 45 kDa antigenic determinants. The level of anti-anti-McAbs varied from titers 1:100 to 1:1600 at different time post-immunization with 1E2. The results provide evidence to understand the appearance of anti-McAb antibody and the way to develop anti-idiotype and anti-anti-idiotype McAb for research purpose.
Liu
S X
SX
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine.
Song
G C
GC
Chen
C Y
CY
Ding
L Y
LY
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000909:Antibodies, Helminth / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D011817:Rabbits; D012549:Schistosoma japonicum / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal