Effects of intracerebroventricularly administered neostigmine on sympathetic neural activities of peripheral tissues in rats.
Sympathetic nervous activity (SNA) in the liver, heart, pancreas and interscapular brown adipose tissue was examined 60 min after the third cerebroventricular injection of neostigmine (5 x 10(-8) mol) in rats. We employed the technique of specific gas chromatography-mass spectrometry for simultaneous analysis of norepinephrine (NE) and its primary neuronal metabolite, 3,4-dihydroxyphenylethyleneglycol (DHPG) and used the ratio DHPG/NE as an index of SNA. Neostigmine produced significant increases in the DHPG/NE ratio in all tissues investigated. Co-administration of atropine with neostigmine completely inhibited this neostigmine-induced effect. These findings suggest that the central cholinergic-muscarinic activation with neostigmine stimulates SNA in the peripheral tissues examined.
Gotoh
M
M
Garvan Institute of Medical Research, St. Vincent's Hospital, Sydney, Australia.
Smythe
G A
GA
D002001:Adipose Tissue, Brown / Q000294:innervation; D000818:Animals; D001285:Atropine / Q000008:administration & dosage / Q000494:pharmacology; D002552:Cerebral Ventricles / Q000187:drug effects / Q000502:physiology*; D006329:Heart Conduction System / Q000187:drug effects; D007276:Injections, Intraventricular; D008099:Liver / Q000294:innervation; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000378:metabolism; D009388:Neostigmine / Q000008:administration & dosage / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009638:Norepinephrine / Q000378:metabolism; D009928:Organ Specificity; D010179:Pancreas / Q000294:innervation; D051381:Rats; D011919:Rats, Inbred Strains; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000502:physiology*
D009388:Neostigmine; D008734:Methoxyhydroxyphenylglycol; D001285:Atropine; C010117:3,4-dihydroxyphenylglycol; D009638:Norepinephrine