[The inhibition of mitosis in tissue culture cells by blue light].
Blue light (wavelength 350-480 nm) irradiation of the early mitotic (prophase and prometaphase) tissue culture cells at the dose of 50-3000 J/cm2 delay mitosis or completely block it at the metaphase. Cell sensitivity to the near UV light (wavelength 360 nm) was few times more as compared with the sensitivity to the visible light (wavelength 400-480 nm). Mitotic cells irradiated with the green light (wavelength more than 500 nm; dose up to 7500 J/cm2) completed division normally. The effect of the blue light did not depend on the presence of phenol red in tissue culture medium. Rhodamin 123 staining did not show any changes in the mitochondrial system in the irradiated mitotic cells. Blue light irradiation with the dose enough for the induction of mitotic delay appears to be insufficient to affect the proliferation of interphase cells.
Gorgidze
L A
LA
Oshemkova
S A
SA
Vorob'ev
I A
IA
D000818:Animals; D002455:Cell Division / Q000528:radiation effects; D002478:Cells, Cultured / Q000166:cytology / Q000528:radiation effects; D003116:Color; D008027:Light; D008856:Microscopy, Fluorescence; D008938:Mitosis / Q000528:radiation effects*; D014466:Ultraviolet Rays
Ingibirovanie mitoza v kletkakh kul'tury tkani sinim svetom.