[Dependence of human erythrocyte methemoglobin reductase on temperature].
The effect of temperature on the activities of cytoplasmic and membrane-bound fractions of NADH-cytochrome beta 5 reductase on the total activity of methemoglobin reductase in intact human erythrocytes was studied within the temperature range of 20-50 degrees C. The above three activities showed a break in the Arrhenius plots at 42 degrees C which was attributed to irreversible inactivation of the enzymes. Thermal inactivation of methemoglobin reductase in erythrocytes was found to increase the methemoglobin content concomitantly with a decrease in the osmotic stability and activation of spontaneous cell hemolysis.
Kozlova
N M
NM
Chernitskiĭ
E A
EA
D003579:Cytochrome Reductases / Q000378:metabolism; D042966:Cytochrome-B(5) Reductase / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000201:enzymology; D004912:Erythrocytes / Q000201:enzymology*; D006454:Hemoglobins / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D008706:Methemoglobin / Q000378:metabolism; D013696:Temperature
D006454:Hemoglobins; D008706:Methemoglobin; D003579:Cytochrome Reductases; D042966:Cytochrome-B(5) Reductase
Zavisimost' aktivnost' metgemoglobinreduktazy éritrotsitov cheloveka ot temperatury.