title: Use of a collagen coated vicryl tube in reconstruction of the porcine esophagus.
abstract: This experimental study assessed the use of a collagen-coated vicryl mesh tube to reconstruct the esophagus in growing piglets. Initial experiments, in which a segment of thoracic esophagus was excised and replaced by this prosthetic tube, resulted in all the animals succumbing to mediastinitis within the first 3-4 days. This was shown, at post-mortem, to be due to leakage of the prosthesis, secondary to acid reflux, resulting in dissolution of the prosthesis. The collagen-coated vicryl mesh was thereafter treated with glutaraldehyde and in-vitro studies showed that the glutaraldehyde-treated material exhibited a higher resistance to 0.01 M HCl at pH 2.0, when compared with untreated material. In addition, the glutaraldehyde-treated material also showed an increased resistance to digestion by bacterial collagenase. Further experiments to reconstruct the cervical esophagus in growing piglets were performed using the revised glutaraldehyde cross-linked collagen coated vicryl mesh tube. All the animals survived the procedure and the prosthesis were leakproof immediately post-operatively. The animals, however, developed severe stenosis at a mean of 11 days post-operatively. Attempts at neo-epithelialization of the graft were seen histologically. There was considerable granulation tissue and scar tissue formation on the mediastinal aspect of the graft. It is suggested that collagen coated vicryl mesh tube may find applications in the treatment of esophageal atresia only if the problem of stenosis of the prosthesis can be solved.
authors.affiliation: Department of Pediatric Surgery, Royal Hospital for Sick Children, Yorkhill, Glasgow.
authors.forename: A D
authors.initials: AD
authors.lastname: Purushotham
authors.forename: R
authors.initials: R
authors.lastname: Carachi
authors.forename: S D
authors.initials: SD
authors.lastname: Gorham
authors.forename: D A
authors.initials: DA
authors.lastname: French
authors.forename: A A
authors.initials: AA
authors.lastname: Shivas
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D003094:Collagen / Q000627:therapeutic use*; D004933:Esophageal Atresia / Q000601:surgery; D004944:Esophagoplasty / Q000379:methods*; D011098:Polyglactin 910; D011183:Postoperative Complications; D019736:Prostheses and Implants; D013526:Surgical Mesh; D013552:Swine
chemical_list: C048470:glutaraldehyde-cross-linked collagen; D011098:Polyglactin 910; D003094:Collagen