title: Solid-phase gene assembly.
abstract: New technologies for multiple chemical synthesis of oligonucleotides and stepwise hybridization on a solid-phase support enable the rapid and cost-effective preparation of long duplex DNA regions. Will these new technologies usher in a new era in protein engineering?
authors.affiliation: Center for Biotechnology, Baylor College of Medicine, The Woodlands, Texas 77381.
authors.forename: K L
authors.initials: KL
authors.lastname: Beattie
authors.forename: R F
authors.initials: RF
authors.lastname: Fowler
mesh_terms: D004247:DNA / Q000138:chemical synthesis* / Q000235:genetics; D005813:Genes, Synthetic; D005818:Genetic Engineering / Q000379:methods*; D016045:Human Genome Project; D006801:Humans; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis; D015202:Protein Engineering / Q000379:methods
chemical_list: D009841:Oligonucleotides; D004247:DNA