Promoters of auxin-induced genes from tobacco can lead to auxin-inducible and root tip-specific expression.
In previous studies we have identified several mRNAs which accumulate after addition of 2,4-dichlorophenoxyacetic-acid (2,4-D) to auxin-starved tobacco cells. The mRNAs corresponding to cDNA clone pCNT103 were found to accumulate transiently prior to the cell division response due to auxin treatment. In this study we determined the sequences of three 103-like cDNAs and two 103-like genes, GNT1 and GNT35. To further study the regulation of the expression of these genes their 5' regions were translationally fused with the beta-D-glucuronidase reporter gene (GUS). The GNT1 5' region led to GUS expression only in the root tips of transgenic plants. By using transgenic hairy-root cultures and transformed cell suspension cultures it was shown that the 5' regions of both GNT1 and GNT35 lead to 2,4-D-inducible expression of GUS activity. The homology of the 103-like genes with other auxin-regulated genes is evaluated.
van der Zaal
E J
EJ
Department of Plant Molecular Biology, Leiden University, Netherlands.
Droog
F N
FN
Boot
C J
CJ
Hensgens
L A
LA
Hoge
J H
JH
Schilperoort
R A
RA
Libbenga
K R
KR
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000187:drug effects*; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics* / Q000502:physiology
D011993:Recombinant Fusion Proteins; D015084:2,4-Dichlorophenoxyacetic Acid; D005966:Glucuronidase