High-performance liquid chromatographic determination of 4,4'-methylenedianiline in human urine.
A method is described for the determination of urinary 4,4'-methylenedianiline (MDA) by high-performance liquid chromatography (HPLC). MDA was extracted from hydrolyzed urine using C18 solid-phase extraction columns. The extract was analyzed by reversed-phase HPLC with electrochemical detection at a cell potential of 0.8 V. The method was very sensitive (detection limit 2.5 micrograms/l) and quantitation using 4,4'-ethylenedianiline as an internal standard correlated well with results by gas chromatography-mas spectrometry. Run-to-run precision (n = 25) averaged 8.9%. In analysis of more than 160 potentially exposed workers, MDA was detected in less than 20% of the urines and concentrations ranged up to 210 micrograms MDA per g of creatinine.
Peterson
J C
JC
Pacific Toxicology Laboratories, Los Angeles, CA 90025.
Estiva
E C
EC
Lyttle
D S
DS
Harris
R M
RM
D000814:Aniline Compounds / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006868:Hydrolysis; D001559:Benzhydryl Compounds
D000814:Aniline Compounds; C009505:4,4'-diaminodiphenylmethane; D001559:Benzhydryl Compounds