title: Structural dynamics and functional domains of the fur protein.
abstract: Proteolytic enzymes were used to detect metal-induced conformational changes in the ferric uptake regulation (Fur) protein of Escherichia coli K12. Metal binding results in enhanced cleavage of the N-terminal region of Fur by trypsin and chymotrypsin. Activation of both trypsinolysis sensitivity and DNA binding have similar metal ion specificity and concentration dependencies, suggesting that the conformational change detected is required for operator DNA binding. Isolation and characterization of biochemically generated fragments of Fur as well as other data indicate that the N-terminal region is necessary for the interaction of the repressor with DNA and that a C-terminal domain is sufficient for binding to metal ions.
authors.affiliation: Department of Molecular and Cellular Biology, University of California, Berkeley 94720.
authors.forename: M
authors.initials: M
authors.lastname: Coy
authors.forename: J B
authors.initials: JB
authors.lastname: Neilands
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002104:Cadmium / Q000494:pharmacology; D002712:Chlorides; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000032:analysis; D006868:Hydrolysis; D007501:Iron / Q000378:metabolism*; D008345:Manganese / Q000494:pharmacology; D017895:Manganese Compounds; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014357:Trypsin; D015032:Zinc / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D002712:Chlorides; D004268:DNA-Binding Proteins; D017895:Manganese Compounds; D010446:Peptide Fragments; D012097:Repressor Proteins; C068477:ferric uptake regulating proteins, bacterial; D002104:Cadmium; D008345:Manganese; D007501:Iron; D014357:Trypsin; D015032:Zinc; C025340:manganese chloride