Quantitative analysis of limonin in grapefruit juice using an enzyme-linked immunoassay: interlaboratory study.
Eleven laboratories participated in an interlaboratory study of an enzyme-linked immunoassay for limonin in grapefruit juice. Participating laboratories received training and practice samples until familiar with the procedure. Laboratories then received 8 sample pairs of grapefruit juice as blind duplicates. Samples were refrigerated and analyzed within 4 days of receipt. Results from 1 laboratory and 6 individual samples were excluded from the statistical analysis. Method performance was measured by the one-way component of variance analysis. Repeatability relative standard deviations (RSDr) ranged from 7.0 to 15.6%. Reproducibility relative standard deviations (RSDR) ranged from 12.9 to 29.4%.
Widmer
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Florida Department of Citrus, Lake Alfred 33850.
Rouseff
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RL
D001628:Beverages / Q000032:analysis*; D002957:Citrus / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D036701:Limonins; D014315:Triterpenes / Q000032:analysis*
D007074:Immunoglobulin G; D007202:Indicators and Reagents; D036701:Limonins; D014315:Triterpenes; C001546:limonin