Synchronized meiosis and recombination in fission yeast: observations with pat1-114 diploid cells.
The mutation pat1-114 has been used to synchronize meiosis in the fission yeast Schizosaccharomyces pombe. We have investigated several aspects of such synchronized meiotic cultures. In both pat1-114 and pat1+ diploids, meiotic landmark events are initiated at the same time after meiosis induction, but synchrony is much more pronounced in the pat1-114-driven meiosis. Commitment to recombination and to meiosis have been timed at 2 h after meiotic induction. Due to a seven-fold reduction of intragenic recombination frequency in the ade6 region of pat1-114 diploids, physical analysis of recombination has not been possible. We have distinguished three factors that influence intragenic recombination frequencies: temperature, azygotic versus zygotic meiosis, and the nature of the pat1 allele. Differences and similarities in the timing of meiotic landmarks in S. cerevisiae and S. pombe are discussed.
Bähler
J
J
Institute of General Microbiology, University of Berne, Switzerland.
Schuchert
P
P
Grimm
C
C
Kohli
J
J
D002453:Cell Cycle; D004171:Diploidy; D008540:Meiosis / Q000235:genetics; D011995:Recombination, Genetic; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development