title: Cloning of alginate lyase gene (alxM) and expression in Escherichia coli.
abstract: The alxM gene encoding a D-mannuronan-specific alginate lyase has been cloned from a marine bacterium isolated as an epiphyte on the brown alga, Sargassum fluitans. Expression of this gene in Escherichia coli provides a source of this enzyme for probing alginate structure and modifying the mannuronan-rich alginate polymers produced by bacterial pathogens.
authors.affiliation: Department of Chemistry, Michigan Technological University, Houghton 49931.
authors.forename: B J
authors.initials: BJ
authors.lastname: Brown
authors.forename: J F
authors.initials: JF
authors.lastname: Preston
authors.forename: L O
authors.initials: LO
authors.lastname: Ingram
mesh_terms: D015139:Blotting, Southern; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D006090:Gram-Negative Bacteria / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D000459:Phaeophyceae; D011133:Polysaccharide-Lyases / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D013379:Substrate Specificity
chemical_list: D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase