title: Characterization of N- and C-terminal deletion mutants of the rat serotonin HT2 receptor in Xenopus laevis oocytes.
abstract: Deletion mutants of the serotonin HT2 receptor have been constructed in which the N- and C-terminal sequences have been gradually removed. The mutant constructs were assayed for their biological activity by electrophysiological measurements in Xenopus oocytes previously injected with the respective in vitro synthesized cRNAs. No significant loss of biological activity was observed when either the extracellular N-terminal sequence, including all potential glycosylation sites, up to the beginning of the first transmembrane domain or the C-terminal sequence up to cystein residue 397, which is conserved in most G-protein coupled receptor known so far, was deleted from the serotonin HT2 receptor constructs.
authors.affiliation: Institut für Zellbiochemie, UKE, Universität Hamburg, Hamburg, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Buck
authors.forename: W
authors.initials: W
authors.lastname: Meyerhof
authors.forename: H
authors.initials: H
authors.lastname: Werr
authors.forename: D
authors.initials: D
authors.lastname: Richter
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D002872:Chromosome Deletion; D004594:Electrophysiology; D006031:Glycosylation; D008564:Membrane Potentials / Q000187:drug effects; D008969:Molecular Sequence Data; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011985:Receptors, Serotonin / Q000235:genetics* / Q000502:physiology; D012701:Serotonin / Q000494:pharmacology; D014158:Transcription, Genetic; D014982:Xenopus laevis
chemical_list: D012333:RNA, Messenger; D011985:Receptors, Serotonin; D012701:Serotonin