Isolation, physical map and gene map of mitochondrial DNA from the cryptomonad Pyrenomonas salina.
Mitochondrial DNA (mtDNA) from the cryptomonad Pyrenomonas salina was isolated by CsCl-buoyant density centrifugation of whole-cell DNA in the presence of Hoechst dye 33258. mtDNA consists of circular molecules about 47 kb in size as estimated from restriction enzyme analysis. A physical map for six restriction enzymes (Bam HI, Bge I, Eco RI, Pst I, Sac I and Sal I) has been constructed. Genes coding for the small subunit of rRNA, cytochrome oxidase subunits I and II, and apocytochrome b were localized on this map using Southern blot hybridization with heterologous gene probes from Oenothera. Genes for 5S rRNA and NADH dehydrogenase subunit 5 are absent from P. salina mtDNA. The mitochondrial genome, being the first analysed to this extent in chromophytic algae, should be valuable for taxonomic and phylogenetic studies.
Maerz
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Institut für Biologie II, Lehrstuhl für Zellbiologie, Freiburg, Germany.
Sitte
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D002499:Centrifugation, Density Gradient; D004247:DNA / Q000302:isolation & purification; D004270:DNA, Circular / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics; D056890:Eukaryota / Q000235:genetics*; D009245:NADH Dehydrogenase / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D015183:Restriction Mapping
D004270:DNA, Circular; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D012341:RNA, Ribosomal, 5S; D004247:DNA; D009245:NADH Dehydrogenase