Differences between embryonic and adult Torpedo acetylcholine receptor gamma subunit.
Antibodies to a synthetic peptide corresponding to residues 346-359 of the Torpedo acetylcholine receptor (AChR) gamma subunit, were employed to compare the adult and embryonic receptor. This peptide contains a consensus phosphorylation site for cAMP-dependent protein kinase (PKA). The anti-peptide antibodies discriminated between adult and embryonic AChRs, and reacted preferentially with the adult gamma form. These observed immunological differences did not seem to stem from different phosphorylation states. Our results suggest that the embryonic Torpedo AChR may have a gamma-like subunit that differs from the known adult form of this subunit, at least in the specific region that contains the phosphorylation site for PKA.
Souroujon
M C
MC
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Carmon
S
S
Safran
A
A
Fuchs
S
S
D000595:Amino Acid Sequence; D000818:Animals; D004557:Electric Organ / Q000737:chemistry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry*; D010766:Phosphorylation; D011950:Receptors, Cholinergic / Q000737:chemistry*; D016415:Sequence Alignment; D014101:Torpedo / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*
D010446:Peptide Fragments; D011950:Receptors, Cholinergic