title: In vivo antigen expression by Pasteurella multocida.
abstract: Pasteurella multocida was purified from the blood of turkeys affected with acute fowl cholera, and membrane preparations from those bacteria were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and visualized on immunoblots. Antigens were detected in the membranes of these in vivo-propagated bacteria that were not detected in membrane preparations of the same P. multocida strain grown in vitro. The unique antigens were detected in the detergent-insoluble phase and were enriched to various degrees by different detergents.
authors.affiliation: Department of Avian Medicine, University of Georgia, Athens 30602.
authors.forename: J R
authors.initials: JR
authors.lastname: Glisson
authors.forename: I H
authors.initials: IH
authors.lastname: Cheng
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D010325:Pasteurella / Q000276:immunology*; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary*; D011201:Poultry Diseases / Q000382:microbiology*; D014422:Turkeys
chemical_list: D000956:Antigens, Viral