[Changes in cell kinetics of rat lens epithelial cells with aging].
Whole-mount preparations of developing rat lens epithelium were made and 3H-thymidine autoradiography was performed on them. The distribution of 3H-thymidine-labeled (DNA-synthesizing) epithelial cells was examined by the above combined methods. Labeled cells were evenly distributed in the epithelial cell layer (not the definitely germinal zone) until 1 week after birth. In 3- or 7-week-old rat lens, labeled cells were mainly distributed in the germinal zone, and the number of labeled cells localized in the anterior subcapsular epithelial cell layer decreased according to age of the rat. The 24-week-old rat showed localization of labeled cells at the germinal zone, which seemed to be similar to the human adult lens.
Terubayashi
H
H
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan.
Tsuji
T
T
Ibaraki
N
N
Mori
K
K
Ikebe
H
H
Akagi
Y
Y
D000375:Aging / Q000473:pathology*; D000818:Animals; D001345:Autoradiography; D002453:Cell Cycle; D004848:Epithelium / Q000473:pathology; D007908:Lens, Crystalline / Q000473:pathology*; D008297:Male; D051381:Rats