A new monoclonal antibody to human subcapsular thymic epithelial cells.
A monoclonal antibody, termed K-20, was generated against an anaplastic thymic carcinoma cell line, Ty-82. Subcapsular thymic epithelial cells of the thymus and blood vessels in various organs were shown to react with the K-20 monoclonal antibody by immunohistochemical staining. Immunofluorescent study revealed that various haematopoietic fresh cells and cell lines did not show any significant reactivity with K-20, except for one Epstein-Barr-virus-carrying lymphoma cell line (SP-50B). Western immunoblotting and affinity purification procedure revealed that K-20 was directed to a protein with a molecular weight of 28 kDa. K-20 is unique in its restrictive reactivity with human subcapsular thymic epithelial cells.
Takeuchi
T
T
Department of Internal Medicine, Kochi Medical School, Japan.
Kubonishi
I
I
Ohtsuki
Y
Y
Miyoshi
I
I
D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000032:analysis*; D002460:Cell Line; D004848:Epithelium / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology; D013953:Thymus Neoplasms / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens