Rapid isolation of a neurohormone from mosquito heads by high-performance liquid chromatography.
Methods were developed for the isolation of the egg development neurosecretory hormone, EDNH, from heads of the mosquito Aedes aegypti. This hormone stimulates ecdysone production by ovaries. Methods used for the successful isolation of insulin-like peptides from vertebrate tissues were modified to develop a four-step procedure involving extraction in acidified ethanol, precipitation by neutralization, followed by sequential separation on size-exclusion, ion-exchange and reversed-phase high-performance liquid chromatography columns.
Wheelock
G D
GD
Department of Entomology, Cornell University, Ithaca, NY 14853.
Sieber
K P
KP
Hagedorn
H H
HH
D000330:Aedes / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006257:Head; D007301:Insect Hormones / Q000302:isolation & purification* / Q000494:pharmacology; D007814:Larva / Q000032:analysis; D009865:Oocytes / Q000187:drug effects
D007301:Insect Hormones