Effect of nutritive elements on the extracellular protein of different Bacillus strains, toxic to mosquito larvae.
The effect of 5 nutritional elements (glucose, peptone, yeast extract, beef extract and lactic acid) on the growth of the 4 tested Bacillus strains (Bacillus thuringiensis Bactimos 78, B. thuringiensis IPS 82, B. sphaericus Solvay 85 and B. sphaericus Rb 80) as well as on the concentration of their extracellular proteins were studied. The nutritive elements were individually tested either dissolved in distilled water or in a buffer base. Results indicated that the bacterial growth was higher when the nutritive elements were dissolved in a buffer base than in dist. water. The concentration of the extracellular proteins were always higher in the absence of the inorganic minerals, although the toxicity of these extracellular proteins against 3rd instar Culex pipiens larvae increased in the presence of these inorganic elements.
Rady
M H
MH
Department of Entomology, Faculty of Science, Ain Shams University, Abbassia, Cairo.
el-Deen
A F
AF
D000818:Animals; D001407:Bacillus / Q000254:growth & development*; D001413:Bacillus thuringiensis / Q000254:growth & development*; D001427:Bacterial Toxins / Q000096:biosynthesis*; D003465:Culex; D003470:Culture Media; D007814:Larva
D001427:Bacterial Toxins; D003470:Culture Media