[Effect of pH and preparation method on thermal denaturation of thymus DNA in salt-free solutions].
Thermal denaturation of calf thymus DNA was studied by scanning microcalorimetry. It has been shown that long dialysis of DNA against water causes a sharp decrease of DNA thermal stability due to biopolymer protonation. The literature data on the properties of DNA salt-free solutions obtained by dialysis are discussed.
Vlasov
A P
AP
Andrianov
V T
VT
D000818:Animals; D001704:Biopolymers; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D009691:Nucleic Acid Denaturation; D012996:Solutions
D001704:Biopolymers; D012996:Solutions; D004247:DNA
Vliianie pH i metoda polucheniia na termicheskuiu denaturatsiiu timusnoĭ DNK v bessolevykh rastvorakh.