title: [Long-term culture and a morphological study of endothelial cells from human aorta].
abstract: Endothelial cells from human aorta were successfully cultured first in this Lab in our country. Cells survived and passed through 10-15 generations. Long term cultured endothelial cells from human aorta were observed under phase-contrast microscope, scanning, transmission electron microscope, and investigated immunocytochemically by immunofluorescence of specific antibody against Factor-VIII related surface antigen, and ABC method using monoclonal antibody 9B9 against human angiotensin-converting enzyme. Medium RPMI-1640 supplemented with 20% human serum, endothelial cell growth factor 200 micrograms/ml, heparin 100 micrograms/ml and gelatin coated flasks were very important conditions for long term culture of human endothelial cell.
authors.affiliation: Laboratory of Experimental Pathology, China Medical University, Shenyang.
authors.forename: Y
authors.initials: Y
authors.lastname: Dong
mesh_terms: D001011:Aorta / Q000166:cytology*; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D013997:Time Factors
chemical_list: D003470:Culture Media