Human spermatozoal FA-1 binds with ZP3 of porcine zona pellucida.
Experiments were carried out to study the binding of the major porcine zona pellucida glycoprotein ZP3 (Mr = 55,000) with human FA-1, a fertilization-related antigen isolated from human male germ cell plasma membranes and with GA-1, a germ cell antigen isolated from murine male germ cell plasma membranes. FA-1 showed a dose-dependent binding with ZP3 in an enzyme-linked immunosorbent assay (ELISA) and by the Western blot procedure. FA-1 also bioneutralized the sperm ligand activity of ZP3 in a boar sperm/porcine zona pellucida attachment assay. In contrast, GA-1 displayed no reactivity for ZP3 using any of these procedures. These results indicate that FA-1 but not GA-1 has zona pellucida binding activity in the porcine system.
Naz
R K
RK
Department of Obstetrics and Gynecology, Albert Einstein College of Medicine, Bronx, NY 10461.
Sacco
A G
AG
Yurewicz
E C
EC
D000941:Antigens / Q000276:immunology*; D003269:Contraception, Immunologic; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005306:Fertilization / Q000276:immunology; D006801:Humans; D008297:Male; D013084:Sperm-Ovum Interactions / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology; D015044:Zona Pellucida / Q000276:immunology*
D000941:Antigens; C054151:fertilization antigen