title: 31P NMR spectroscopy of 9L cell line in culture: differential effects of high temperature on anchored cells and spheroids.
abstract: The differential effects of high temperature on 9L rat gliosarcoma cells cultured in two distinctive forms, namely, anchored monolayer cells and multicellular spheroids, were investigated using 31P nuclear magnetic resonance spectroscopy. The critical temperatures resulting in irreversible changes in cellular energetics were found to be significantly different: 45 degrees C for anchored cells and 43 degrees C for multicellular spheroids, respectively. Phosphomonoester levels in multicellular spheroids were found to be consistently higher than those in anchored monolayer cells regardless of the temperature to which they were exposed. The study demonstrates that tissue derived from a single cell line may exhibit different metabolic properties depending on its growth pattern.
authors.affiliation: Neurochemistry Research Laboratory, Department of Veterans Affairs Medical Center, Martinez, California 94553.
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
authors.forename: I L
authors.initials: IL
authors.lastname: Kwee
authors.forename: L B
authors.initials: LB
authors.lastname: Tamas
authors.forename: T
authors.initials: T
authors.lastname: Nakada
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004734:Energy Metabolism; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006358:Hot Temperature; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008863:Microspheres; D010477:Perfusion; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus; D051381:Rats; D013997:Time Factors
chemical_list: D010710:Phosphates; D010758:Phosphorus