Determination of pentoxifylline in serum by high-performance thin-layer chromatography.
An analytical method to study the bioavailability of newly developed pentoxifylline sustained-release tablets has been developed and assessed in experiments on dogs. For the isolation of pentoxifylline and its metabolites from serum solid-liquid extraction was applied by involving the internal standard probe. HPTLC plates with a preconcentration zone were used for separation of the analysed substances, using chloroform-methanol (95:5, v/v). Quantification was by densitometric detection. The detector response was linear in the concentration range investigated for pentoxifylline: 0.02-1.5 micrograms ml-1 of serum.
Bauerová
K
K
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Soltés
L
L
Kállay
Z
Z
Schmidtová
K
K
D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D002855:Chromatography, Thin Layer; D003692:Delayed-Action Preparations; D004285:Dogs; D006207:Half-Life; D007275:Injections, Intravenous; D010431:Pentoxifylline / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics
D003692:Delayed-Action Preparations; D010431:Pentoxifylline