[The use of microdot immunoenzyme analysis with visual detection for the determination of tularemia antibodies].
The possibility of using the micropoint enzyme immunoassay (EIA) on a nitrocellulose membrane with the visual evaluation of results for the detection of tularemia IgG antibodies in hamadryas baboons at the postvaccinal period has been studied. The sensitivity of this assay has been compared with that of the passive hemagglutination (PHA) test, the microagglutination (MA) test and EIA with the spectrophotometric evaluation of results in plates. As shown in this study, EIA in the above-mentioned modification can be successfully used for the detection of tularemia antibodies in the blood serum. The sensitivity of micropoint EIA has proved to be not inferior to that of EIA in plates, while exceeding the sensitivity of the PHA test 10- to 20-fold and the sensitivity of the MA test 10- to 1,000-fold. This method is simple, reliable, highly sensitive, economic and requires no special equipment, which makes it highly promising for the diagnosis of tularemia and the evaluation of humoral immunity at the postvaccinal period.
Iurov
S V
SV
Pchelintsev
S Iu
SIu
Afanas'ev
S S
SS
Vorob'ev
A A
AA
Urakov
N N
NN
Cherenkova
G V
GV
Fedortsov
K K
KK
Krasnoproshina
L I
LI
Vlasov
G S
GS
Denisova
N B
NB
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001428:Bacterial Vaccines / Q000276:immunology; D003101:Collodion; D005069:Evaluation Studies as Topic; D005260:Female; D005604:Francisella tularensis / Q000276:immunology*; D007114:Immunization / Q000379:methods; D015151:Immunoblotting / Q000295:instrumentation / Q000379:methods*; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D008297:Male; D008567:Membranes, Artificial; D010215:Papio; D013997:Time Factors
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D008567:Membranes, Artificial; D003101:Collodion
Ispol'zovanie mikrotochechnogo immunofermentnogo analiza s vizual'noĭ indikatsieĭ dlia opredeleniia tuliaremiĭnykh antitel.