title: Comparison of the antimicrobial effect of the application of chlorhexidine gel, amine fluoride gel and stannous fluoride gel in debrided periodontal pockets.
abstract: The clinical and microbiological effect of locally-applied chlorhexidine gel 2%, amine fluoride gel 1.25%, stannous fluoride gel 4% or placebo gel in 40 periodontal pockets of 10 patients were studied. The gels were applied 3 x within 10 min. after mechanical debridement of the pockets. The treatment effect on the subgingival microflora was evaluated by microscopic and culture studies of the subgingival plaque samples. In addition, supragingival plaque, bleeding after probing and probing pocket depth were scored. Examination were carried out before and during a period of 36 weeks after treatment. At the start, the cultured microflora consisted mainly of anaerobic Gram-positive bacteria. Following treatment, the clinical parameters were significantly reduced. Concomitantly, the %s of spirochetes, motile rods and non-motile rods decreased significantly. A significant decrease was also found in the total anaerobic count, whereas the facultative counts remained at the same level found before treatment. This suggested that the treatment resulted in a mainly facultative subgingival microflora. The % Gram-negative rods showed a significant reduction after treatment, but returned to base line at week 12. Statistical analysis of the bacteriological and clinical examinations failed to demonstrate any significant differences between the 4 treatment groups. Thus, in comparison to the placebo gel, subgingival application of chlorhexidine gel 2%, amine fluoride gel 1.25% or stannous fluoride gel 4% did not augment the effect of mechanical debridement on bacteriological and clinical parameters during the experimental period of 36 weeks. However the indicated treatments resulted in a facultative subgingival microflora which is compatible with the host.
authors.affiliation: Department of Periodontology and Preventive Dentistry, University of Nijmegen, The Netherlands.
authors.forename: P J
authors.initials: PJ
authors.lastname: Oosterwaal
authors.forename: F H
authors.initials: FH
authors.lastname: Mikx
authors.forename: M A
authors.initials: MA
authors.lastname: van 't Hof
authors.forename: H H
authors.initials: HH
authors.lastname: Renggli
mesh_terms: D000328:Adult; D000588:Amines / Q000008:administration & dosage / Q000627:therapeutic use*; D001419:Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D002710:Chlorhexidine / Q000008:administration & dosage / Q000627:therapeutic use*; D003773:Dental Plaque / Q000382:microbiology / Q000517:prevention & control; D003959:Diamines; D004311:Double-Blind Method; D005260:Female; D005459:Fluorides / Q000008:administration & dosage / Q000627:therapeutic use*; D005782:Gels; D005884:Gingival Hemorrhage / Q000517:prevention & control; D006801:Humans; D008297:Male; D008875:Middle Aged; D010514:Periodontal Pocket / Q000188:drug therapy* / Q000382:microbiology; D010518:Periodontitis / Q000188:drug therapy / Q000382:microbiology; D010919:Placebos; D013357:Subgingival Curettage; D014002:Tin Fluorides / Q000008:administration & dosage / Q000627:therapeutic use*; D014464:Ultrasonic Therapy
chemical_list: D000588:Amines; D003959:Diamines; D005782:Gels; D010919:Placebos; D014002:Tin Fluorides; C495399:Olaflur; D005459:Fluorides; D002710:Chlorhexidine