Isolation of exfoliative toxin from Staphylococcus hyicus subsp. hyicus and its exfoliative activity in the piglet.
Exfoliative toxin was isolated from the sterile cell-free filtrate of 24 h culture of Staphylococcus hyicus subsp. hyicus strain P-1. The partial purification of exfoliative toxin produced by S. hyicus (shET) was performed by precipitation with 50-80% saturated ammonium sulfate, gel filtration on a Sephadex G-75 column and column chromatography on DEAE-cellulose. Partially purified shET (pp-shET) caused exfoliation in piglets at 8 to 12 h after intradermal or subcutaneous injection. However, heat-treated pp-shET did not cause exfoliation in piglets for up to 24 h after injection. On histopathological examination of the skin at 12 h after injection of pp-shET, an intraepidermal cleavage plane was shown between the stratum corneum and stratum granulosum and at the stratum granulosum.
Sato
H
H
Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
Tanabe
T
T
Kuramoto
M
M
Tanaka
K
K
Hashimoto
T
T
Saito
H
H
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005083:Exfoliatins / Q000008:administration & dosage / Q000302:isolation & purification* / Q000633:toxicity; D006358:Hot Temperature; D007271:Injections, Intradermal / Q000662:veterinary; D007279:Injections, Subcutaneous / Q000662:veterinary; D009336:Necrosis; D012867:Skin / Q000473:pathology*; D013210:Staphylococcus; D013552:Swine
D005083:Exfoliatins