The effects of saikosaponin-d on yeast phagocytosis and degradation in peritoneal macrophages: related increase in Fc receptor expression and altered cytoplasmic organization.
The effects of saikosaponin-d (ssd), isolated from Bupleurum radix, on phagocytic functions of mouse peritoneal macrophages were investigated in macrophage cultures after treatment in vivo. We also compared the ultrastructural appearances of macrophages from ssd-treated mice with those from control mice. The macrophages from ssd-treated mice showed a significant increase in phagocytosis, intracellular killing of yeast and acid phosphatase activity. Treatment of mice with ssd also induced the Fc receptor expression in macrophages. The macrophages from ssd-treated mice showed more intense spreading on a glass surface than those from the control mice after a 60 min-incubation. Furthermore, the macrophages from ssd-treated mice seemed to possess a well-developed Golgi apparatus and the large vacuoles. The data suggest that the functional changes in the macrophages from ssd-treated mice are brought about by a modification in the surface membrane and the intracellular distribution.
Ushio
Y
Y
Research Institute of Oriental Medicine, Kinki University, Osaka, Japan.
Abe
H
H
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D003593:Cytoplasm / Q000648:ultrastructure; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis / Q000187:drug effects*; D011961:Receptors, Fc / Q000032:analysis*; D012502:Sapogenins / Q000008:administration & dosage / Q000494:pharmacology*; D012503:Saponins; D015003:Yeasts / Q000502:physiology*
D011961:Receptors, Fc; D012502:Sapogenins; D012503:Saponins; D009828:Oleanolic Acid; D000135:Acid Phosphatase; C025759:saikosaponin D