[The space-time distribution of the mRNA of the nuclear proteins c-myc and P-53 in the development of the clawed toad studied by hybridization in situ].
We studied distribution of mRNA for nuclear protooncogene c-myc and nuclear protein P-53 in mature oocytes and embryos of Xenopus laevis from the stage of fertilization up to the stage of hatching by in situ hybridization with histological sections. mRNA for c-myc was present in all cells of the embryo at all studied developmental stages. Between the stage of fertilization and up to the late blastula, mRNA concentration for c-myc decreased progressively in all embryonic cells. During gastrulation a local increase in the concentration of this messenger was found in dorsal mesoderm and ectoderm. At the stage of neurula increased concentration of mRNA for c-myc was observed in all cells of the embryo but the hybridization signal increased particularly distinctly in cells of the neural tube. In studies of P-53 mRNA distribution hybridization signal was detected only in brain cells after stage 20 of development (after closure of the neural folds) and up to the stage of hatching.
Luk'ianov
S A
SA
Zaraĭskiĭ
A G
AG
D000818:Animals; D001345:Autoradiography; D001757:Blastomeres / Q000378:metabolism; D009028:Morula / Q000378:metabolism; D009693:Nucleic Acid Hybridization / Q000502:physiology*; D009865:Oocytes / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology / Q000378:metabolism*
D016271:Proto-Oncogene Proteins c-myc; D015347:RNA Probes; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53
Izuchenie prostranstvenno-vremennogo raspredeleniia mRNK iadernykh belkov c-myc i P-53 v razvitii shportsevoĭ liagushki metodom gibridizatsii in situ.