title: Dominant and recessive suppressors that restore glucose transport in a yeast snf3 mutant.
abstract: The SNF3 gene of Saccharomyces cerevisiae encodes a high-affinity glucose transporter that is homologous to mammalian glucose transporters. To identify genes that are functionally related to SNF3, we selected for suppressors that remedy the growth defect of snf3 mutants on low concentrations of glucose or fructose. We recovered 38 recessive mutations that fall into a single complementation group, designated rgt1 (restores glucose transport). The rgt1 mutations suppress a snf3 null mutation and are not linked to snf3. A naturally occurring rgt1 allele was identified in a laboratory strain. We also selected five dominant suppressors. At least two are tightly linked to one another and are designated RGT2. The RGT2 locus was mapped 38 cM from SNF3 on chromosome IV. Kinetic analysis of glucose uptake showed that the rgt1 and RGT2 suppressors restore glucose-repressible high-affinity glucose transport in a snf3 mutant. These mutations identify genes that may regulate or encode additional glucose transport proteins.
authors.affiliation: Department of Genetics and Development, Columbia University College of Physicians and Surgeons, New York, New York 10032.
authors.forename: L
authors.initials: L
authors.lastname: Marshall-Carlson
authors.forename: L
authors.initials: L
authors.lastname: Neigeborn
authors.forename: D
authors.initials: D
authors.lastname: Coons
authors.forename: L
authors.initials: L
authors.lastname: Bisson
authors.forename: M
authors.initials: M
authors.lastname: Carlson
mesh_terms: D004252:DNA Mutational Analysis; D005799:Genes, Dominant / Q000235:genetics; D005808:Genes, Recessive / Q000235:genetics; D016153:Genes, Suppressor / Q000235:genetics*; D005816:Genetic Complementation Test; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
chemical_list: D009004:Monosaccharide Transport Proteins; D005947:Glucose