Phospholipase C isozymes in neurons and glial cells in culture: an immunocytochemical and immunochemical study.
Expression of 3 brain isozymes of phospholipase (PLC-beta, PLC-gamma and PLC-delta) was investigated in relation to cell types found in rat CNS cultures. Immunoreactivity of cultured neurons, astrocytes and oligodendrocytes was demonstrated for all of the 3 isozymes by immunocytochemical staining and immunoblotting, with some differences in reaction intensity. Immunoblotting revealed that the level of expression was neurons greater than oligodendrocytes greater than astrocytes for PLC-beta and PLC-gamma, and astrocytes greater than oligodendrocytes greater than neurons for PLC-delta.
Mizuguchi
M
M
Department of Medicine, University of British Columbia, Vancouver, Canada.
Yamada
M
M
Kim
S U
SU
Rhee
S G
SG
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002478:Cells, Cultured; D005333:Fetus; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis*; D009457:Neuroglia / Q000166:cytology / Q000201:enzymology*; D009474:Neurons / Q000166:cytology / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000201:enzymology*; D010738:Type C Phospholipases / Q000032:analysis*
D007527:Isoenzymes; D010738:Type C Phospholipases