Protein-secretory patterns of normal and abnormal human placentas with special reference to human chorionic gonadotropin.
[35S]Methionine-labeled protein-secretory patterns resolved by two-dimensional polyacrylamide gel electrophoresis in abnormal hydatidiform-mole placentas were compared with those in normal full-term placentas with special reference to human chorionic gonadotropin (hCG) by means of immunoblotting and immunoelectron-microscopic techniques. Although basic protein-secretory patterns of both placentas were similar to each other, four polypeptide spots appeared and one spot disappeared in the hydatidiform-mole samples. Among four newly synthesized and secreted spots, three were immunoreacted with anti-hCG serum by an immunoblotting experiment. Ultrastructural localization of hCG showed that the labeling intensity of anti-hCG serum in hydatidiform-mole placentas was much heavier than that in full-terms ones. Particularly, the Golgi apparatus, middle-sized granules and large bodies were highly immunoreactive. The present study reveals that hydatidiform-mole placentas have different protein-secretory functions especially in hCG synthesis and secretion from those of normal pregnancy.
Fukui
Y
Y
Department of Anatomy, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Ueda
H
H
Hamasaki
K
K
Fujimoto
S
S
D001345:Autoradiography; D006063:Chorionic Gonadotropin / Q000032:analysis* / Q000276:immunology; D003470:Culture Media / Q000032:analysis; D046508:Culture Techniques; D003593:Cytoplasm / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006056:Golgi Apparatus / Q000737:chemistry; D006801:Humans; D006828:Hydatidiform Mole / Q000737:chemistry* / Q000648:ultrastructure; D015151:Immunoblotting; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D010920:Placenta / Q000737:chemistry* / Q000648:ultrastructure; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000737:chemistry* / Q000648:ultrastructure
D006063:Chorionic Gonadotropin; D003470:Culture Media