title: Genetic interactions at the FLA10 locus: suppressors and synthetic phenotypes that affect the cell cycle and flagellar function in Chlamydomonas reinhardtii.
abstract: Through the isolation of suppressors of temperature-sensitive flagellar assembly mutations at the FLA10 locus of Chlamydomonas reinhardtii, we have identified six other genes involved in flagellar assembly. Mutations at these suppressor loci, termed SUF1-SUF6, display allele specificity with respect to which fla10- mutant alleles they suppress. An additional mutation, apm1-122, which confers resistance to the plant herbicides amiprophos-methyl and oryzalin, was also found to interact with mutations at the FLA10 locus. The apm1-122 mutation in combination with three fla10- mutant alleles results in synthetic cold-sensitive cell division defects, and in combination with an additional pseudo-wild-type fla10- allele yields a synthetic temperature-sensitive flagellar motility phenotype. Based upon the genetic interactions of these loci, we propose that the FLA10 gene product interacts with multiple components of the flagellar apparatus and plays a role both in flagellar assembly and in the cell cycle.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309-0347.
authors.forename: F G
authors.initials: FG
authors.lastname: Lux
authors.forename: S K
authors.initials: SK
authors.lastname: Dutcher
mesh_terms: D002453:Cell Cycle / Q000235:genetics*; D002696:Chlamydomonas / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D004136:Dinitrobenzenes / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics; D005407:Flagella / Q000378:metabolism* / Q000502:physiology; D016153:Genes, Suppressor / Q000235:genetics*; D007700:Kinetics; D009154:Mutation / Q000235:genetics; D009578:Nitrobenzenes; D009946:Organothiophosphorus Compounds / Q000494:pharmacology; D010641:Phenotype; D013424:Sulfanilamides; D013696:Temperature
chemical_list: D004136:Dinitrobenzenes; D009578:Nitrobenzenes; D009946:Organothiophosphorus Compounds; D013424:Sulfanilamides; C017823:amiprophos methyl; C012465:oryzalin