Improved procedure for the second-derivative spectrophotometric analysis of carboxyhaemoglobin.
We describe a modification of the second-derivative spectrophotometric assay for carboxyhaemoglobin in blood. Using the original procedure we have often observed a time-dependent change in the signal leading to unreliable results. By using phosphate-buffered-saline (pH 7.4) instead of ammonia to dissolve the deoxygenating agent (sodium dithionite), we obtained stable and reproducible second-derivative signals from which the percentage carboxyhaemoglobin in the patients' samples can be calculated.
Verweij
H
H
Department of Clinical Chemistry, Streekziekenhuis Midden-Twente, Hengelo, The Netherlands.
Bonte
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D001774:Blood Chemical Analysis; D002263:Carboxyhemoglobin / Q000032:analysis*; D005069:Evaluation Studies as Topic; D006801:Humans; D013053:Spectrophotometry
D002263:Carboxyhemoglobin