Separation and purification of oligonucleotides using a new bonded-phase packing material.
We describe a new bonded-phase packing material, based upon surface-stabilised microparticulate silica, suitable for the rapid separation and purification of oligonucleotides. Columns packed with this material were demonstrated to give rapid separations of individual oligonucleotide species of up to 44 base units with high purity; agarose gel electrophoresis showed that the products were essentially single bands, with only trace quantities of the (n-1)-mer present. Baseline resolution of the desired oligomer from (n +/- 1)-mer was achieved under preparative loading conditions, where up to 200-300 micrograms of oligonucleotide could be separated. The separation was essentially independent of structure or sequence of the oligonucleotides. The retention mechanism of the oligonucleotides was investigated, and the results used to determine the optimum column configuration and separation conditions.
Edwardson
P A
PA
Division of Biotechnology, Centre for Applied Microbiology and Research, Salisbury, Wiltshire, UK.
Collins
I J
IJ
Scawen
M D
MD
Atkinson
T
T
Cox
G B
GB
Sivakoff
S
S
Stout
R W
RW
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000302:isolation & purification*; D013696:Temperature
D009841:Oligonucleotides