Improved method for the determination of serotonin in plasma by high-performance liquid chromatography using on-line sample pre-treatment.
An improved method for the determination of serotonin in platelet-rich plasma (PRP) and platelet-poor plasma (PPP), by reversed-phase high-performance liquid chromatography with electrochemical detection and direct plasma injection, is described. The chromatographic system comprises a strong cation-exchange pre-column and a C18 analytical column. The method is selective, rapid, simple and sensitive, and offers good reproducibility and recovery. Reference values for serotonin concentrations in healthy adults (n = 10) are 31 nM for PPP and 6 nmol per 10(9) platelets for PRP. The conditions used for the preparation of PRP and PPP may influence the serotonin concentration in PRP.
Takkenberg
B
B
Department of Clinical Chemistry, University of Amsterdam, The Netherlands.
Endert
E
E
van Ingen
H E
HE
Ackermans
M
M
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012701:Serotonin / Q000097:blood*
D012701:Serotonin