The glycosomes of trypanosomes: number and distribution as revealed by electron spectroscopic imaging and 3-D reconstruction.
Computer-aided 3-D reconstruction of trypanosomes from 0.35-micron-thick sections imaged on the Zeiss 902 electron microscope are being used to study the dynamics of cell organization. Segregation of glycolytic enzymes into glycosomes raises questions concerning the distribution and biogenesis of these organelles. Direct counts of glycosomes from Trypanosoma evansi indicate 30-40 per cell and for the closely related T. brucei, 65 per cell. These figures contrast with the estimates of others who have used model-based morphometric methods to obtain a value of 230 per cell.
Tetley
L
L
Department of Zoology, University of Glasgow, U.K.
Vickerman
K
K
D000818:Animals; D007091:Image Processing, Computer-Assisted; D008854:Microscopy, Electron; D015388:Organelles / Q000648:ultrastructure; D014345:Trypanosoma / Q000648:ultrastructure*; D014346:Trypanosoma brucei brucei / Q000648:ultrastructure*