SF-B that binds to a negative element in glutathione transferase P gene is similar or identical to trans-activator LAP/IL6-DBP.
We have previously identified a silencer (negative enhancer) in glutathione transferase P (GST-P) gene which is strongly and specifically induced during hepatocarcinogenesis of the rat. At least three trans-acting factors bind to multiple cis-elements located in this silencer. One of these factors, SF-B (Silencer Factor B) specifically binds to GPS1 (GST-P Silencer 1) and has been cloned by a Southwestern protocol. Analysis of DNA and deduced amino acid sequence reveals that SF-B clone is most likely identical to an IL-6 inducible trans-activator LAP/IL6-DBP. Binding efficiency of SF-B to GPS1 is indistinguishable from that to IL6-responsive element found in C-reactive protein gene. The possibility that SF-B/LAP/IL6-DBP functions as a dual positive and negative regulator is discussed.
Imagawa
M
M
Department of Biochemistry, University of Tokyo Faculty of Medicine, Japan.
Osada
S
S
Koyama
Y
Y
Suzuki
T
T
Hirom
P C
PC
Diccianni
M B
MB
Morimura
S
S
Muramatsu
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA; D005982:Glutathione Transferase / Q000235:genetics*; D015151:Immunoblotting; D015850:Interleukin-6 / Q000494:pharmacology; D007527:Isoenzymes / Q000235:genetics*; D008099:Liver / Q000502:physiology; D008114:Liver Neoplasms, Experimental; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010957:Plasmids; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism
D015850:Interleukin-6; D007527:Isoenzymes; D015345:Oligonucleotide Probes; D015534:Trans-Activators; C070649:silencer factor B; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase; D005982:Glutathione Transferase