[The use of melamine-formaldehyde latex in the automated analysis of immunocompetent cells].
The optimization of the loading of monodispersed melamine-formaldehyde latex with fluorochrome has permitted its use as indicator material, equally suitable both for the visual evaluation of the reaction of phagocytosis and for the automated evaluation of this reaction by means of a scanning fluorescent microscope. The study has shown the possibility of using fluorescent melamine formaldehyde latex for the evaluation of the phagocytosis of mouse peritoneal macrophages and human blood cells, as well as the analysis of the surface receptors of immunocompetent cells.
Ovcharova
E N
EN
Denisov
L A
LA
Sukhomudrenko
A G
AG
D000818:Animals; D003592:Cytophotometry / Q000295:instrumentation; D005557:Formaldehyde; D006801:Humans; D007121:Immunocompetence / Q000276:immunology*; D007202:Indicators and Reagents; D007840:Latex; D007841:Latex Fixation Tests / Q000295:instrumentation / Q000379:methods; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008856:Microscopy, Fluorescence; D010316:Particle Size; D010586:Phagocytes / Q000276:immunology; D011754:Pyronine; D012117:Resins, Synthetic; D014227:Triazines
D007202:Indicators and Reagents; D007840:Latex; D012117:Resins, Synthetic; D014227:Triazines; D005557:Formaldehyde; C011907:melamine; D011754:Pyronine
Ispol'zovanie melaminoformal'degidnogo lateksa v avtomatizirovannom analize immunokompetentnykh kletok.