[Evaluation of the Mycoscreen technique for the detection and quantification of genital mycoplasmas].
A total of 161 samples from genital sources were evaluated using two different methods for genital Mycoplasma isolation: inoculation to urea broth, arginine broth and A7 solid media (standard method) and a new enzymatic method (Mycoscreen Oxoid). The isolation rates for Ureaplasma urealitycum were 42% (standard method) and 49% (Mycoscreen) (sensitivity: 100%; specificity: 88%). The isolation rates for Mycoplasma hominis were 13% (standard method) and 14% (Mycoscreen) (sensitivity: 80%; specificity: 95%). Using the enzymatic method, manufacturer's positive result is defined as a count greater than 10,000 UCC-ml at 24 hours. When we compare the 24 hours results, the concordance rates was 84% (Ureaplasma urealyticum) and 62% (Mycoplasma hominis). Mycoscreen method is a valid and fast technique for the isolation and identification of genital Mycoplasma. It also allows a fast screening of pathologic counts of Ureaplasma urealyticum in genital exudates.
Pascual
A
A
Departamento de Microbiología, Facultad de Medicina, Sevilla.
Arbeloa
M
M
Aznar
J
J
D001431:Bacteriological Techniques; D002584:Cervix Uteri / Q000382:microbiology*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008297:Male; D009178:Mycoplasmataceae / Q000302:isolation & purification*; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D014521:Urethra / Q000382:microbiology*; D014626:Vaginal Smears
D011933:Reagent Kits, Diagnostic
Evaluación del método Mycoscreen para la detección y cuantificación de micoplasmas genitales.