title: O-hydroxyphenylacetaldehyde: a major novel metabolite of coumarin formed by rat, gerbil and human liver microsomes.
abstract: A major novel coumarin metabolite was isolated from rat hepatic microsomal incubations by high-performance liquid chromatography. In the presence of a rat liver cytosolic fraction and NADH it was rapidly metabolized to O-hydroxyphenylethanol. The metabolite co-chromatographed with an authentic sample of O-hydroxyphenylacetaldehyde and its identity was confirmed by mass spectral analysis. The formation of O-hydroxyphenylacetaldehyde from coumarin was NADPH-dependent. It was the major metabolite formed by rat, gerbil and human liver microsomes at a coumarin concentration of 1mM.
authors.affiliation: Department of Physiology & Pharmacology, University of Nottingham, U.K.
authors.forename: J H
authors.initials: JH
authors.lastname: Fentem
authors.forename: J R
authors.initials: JR
authors.lastname: Fry
authors.forename: D A
authors.initials: DA
authors.lastname: Whiting
mesh_terms: D000079:Acetaldehyde / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005849:Gerbillinae; D006801:Humans; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D008862:Microsomes, Liver / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
chemical_list: D003374:Coumarins; D009243:NAD; C070647:2-hydroxyphenylacetaldehyde; C030123:coumarin; D000079:Acetaldehyde