title: Bacteriology of the anal wound after open hemorrhoidectomy. Qualitative and quantitative analysis.
abstract: The purpose of this study is to analyze the size of the bacterial colonies in anal wounds after open hemorrhoidectomy. Twenty patients were studied during predetermined postoperative time periods. Material was collected from the surface and from within the tissue of each patient's three open wounds, intraoperatively, on the 6th, 13th and 20th postoperative days for bacteriologic examination in aerobic, microaerophilic, and anaerobic media. The bacterium most commonly identified was Escherichia coli, followed by Staphylococcus aureus and Staphylococcus epidermidis. Pseudomonas aeruginosa, Enterococcus faecalis, Klebsiella pneumoniae, Proteus vulgaris, and Proteus mirabilis were also identified. Critical indexes of colonization were present since the intraoperative stage (greater than 10(5) bacteria/g of tissue and greater than 10(6) bacteria/ml); obligate anaerobic bacteria were not identified; neither the species nor the number of bacteria, even when critical indexes were present, prevented proper healing. The same bacteria were not necessarily present on the surface and in the tissue; the bacterial load observed among the three wounds (left lateral, right posterior, and right anterior), was the same.
authors.affiliation: Paulista Medical School, São Paulo, Brazil.
authors.forename: P R
authors.initials: PR
authors.lastname: de Paula
authors.forename: M B
authors.initials: MB
authors.lastname: Speranzini
authors.forename: H C
authors.initials: HC
authors.lastname: Hamzagic
authors.forename: D G
authors.initials: DG
authors.lastname: Bassi
authors.forename: M A
authors.initials: MA
authors.lastname: Chacon-Silva
authors.forename: N F
authors.initials: NF
authors.lastname: Novo
authors.forename: S
authors.initials: S
authors.lastname: Goldenberg
mesh_terms: D000328:Adult; D000368:Aged; D001003:Anal Canal / Q000382:microbiology* / Q000601:surgery*; D000704:Analysis of Variance; D001419:Bacteria / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D005260:Female; D006484:Hemorrhoids / Q000601:surgery*; D006801:Humans; D008297:Male; D008875:Middle Aged; D013530:Surgical Wound Infection / Q000382:microbiology* / Q000503:physiopathology; D013997:Time Factors; D014945:Wound Healing