Fluorescent and photoaffinity labeling derivatives of rhizoxin.
A fluorescent probe (D-RZX) and a photoreactive fluorescent probe (AD-RZX) for studying the rhizoxin binding site on tubulin were prepared by the derivatization of rhizoxin (RZX). D-RZX consists of a rhizoxin moiety and a dansyl moiety. AD-RZX has a 5-azidonaphthalene-1-sulfonyl moiety instead of the dansyl moiety of D-RZX. Both D-RZX and AD-RZX bound tubulin in a mutually competitive manner with rhizoxin, indicating their binding to the rhizoxin site on tubulin. AD-RZX bound the rhizoxin site covalently after UV-irradiation, thus showing its usefulness as a photo-affinity probe for labeling of the rhizoxin site.
Sawada
T
T
Institute of Applied Microbiology, University of Tokyo, Japan.
Hashimoto
Y
Y
Li
Y
Y
Kobayashi
H
H
Iwasaki
S
S
D000345:Affinity Labels / Q000138:chemical synthesis / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D005456:Fluorescent Dyes; D007700:Kinetics; D007783:Lactones / Q000378:metabolism; D018942:Macrolides; D008868:Microtubule Proteins / Q000378:metabolism*; D013552:Swine; D014404:Tubulin / Q000378:metabolism*
D000345:Affinity Labels; D005456:Fluorescent Dyes; D007783:Lactones; D018942:Macrolides; D008868:Microtubule Proteins; D014404:Tubulin; C041443:rhizoxin