Characteristic features of the sonicated DNA of Agama agama agama L. (Reptilia, Agamidae) on hydroxyapatite columns, using mouse DNA as a reference.
Hydroxyapatite column chromatography has been used to study some properties of the extensively sheared DNA of the Rainbow lizard, Agama agama agama. Reassociation studies show that the genome has a Cot1/2 of 370. Approximately 15% of the genome is highly repetitive in nature. This repetitive fraction is resolved into thermally stable and less stable fractions. The stable fraction has a base composition of 47% GC, higher than the 40.2% GC for the native DNA. This stable fraction is believed to be of recent origin. Chromatography of the total DNA of the lizard with linear gradients of phosphate buffer containing 1 M urea resolves it into two components which were shown by thermal fractionation, also in the presence of 1 M urea, to vary in base composition. This behaviour may be characteristic of reptilian genomes and may be used as a basis for studying the structural organisation of the reptilian genome.
Adegoke
J A
JA
Department of Zoology, Obafemi Awolowo University, Ile-Ife, Nigeria.
Ighavini
B O
BO
Onuigbo
R O
RO
D000818:Animals; D001482:Base Composition / Q000235:genetics; D002848:Chromatography, DEAE-Cellulose; D004247:DNA / Q000737:chemistry*; D004269:DNA, Bacterial / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D006882:Hydroxyapatites / Q000737:chemistry; D008116:Lizards / Q000235:genetics*; D051379:Mice; D009695:Nucleic Acid Renaturation; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D013010:Sonication; D013696:Temperature; D014508:Urea / Q000737:chemistry
D004269:DNA, Bacterial; D006882:Hydroxyapatites; D014508:Urea; D004247:DNA