Psoralen-photosensitized damage of rat peritoneal exudate cells.
Psoralen-sensitized photodamage (PUVA) of rat peritoneal exudate cells was investigated. Quartz-activated luminol-dependent chemiluminescence (ChL) was registered and the amount of trypan-positive cells was determined. Irradiation of peritoneal exudate cells in the presence of psoralen resulted in a dose-dependent monotonous inhibition of ChL. The reciprocity law of irradiation intensity and duration of irradiation was not valid for the observed inhibition of ChL: the inhibition increased with higher intensity. When psoralen previously photooxidized in ethanol (POP) was added to peritoneal exudate cell suspension, a double-phase response depending on psoralen irradiation dose was obtained: ChL activation was observed at low doses of UVA, ChL inhibition at high doses. Chemiluminescence inhibition correlated well with the increase in the number of trypan-positive cells. It may be supposed that the observed effects of PUVA or POP treatment are caused by cell cytoplasmic membrane damage.
Kyagova
A A
AA
Department of Medical and Biological Physics, 2nd Moscow Medical Institute, USSR.
Korkina
L G
LG
Snigireva
T V
TV
Lysenko
E P
EP
Tomashaeva
S K
SK
Potapenko AYa
D000818:Animals; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D004912:Erythrocytes / Q000187:drug effects* / Q000528:radiation effects; D011564:Furocoumarins / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D010529:Peritoneal Cavity / Q000166:cytology*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D051381:Rats; D014466:Ultraviolet Rays
D011564:Furocoumarins; D011838:Radiation-Sensitizing Agents