Plasmids with easily excisable cat gene cartridges.
New cat-cassette-containing vectors based on kanamycin-resistance-encoding plasmids, pHSG298/299, were constructed. They were designed to facilitate the subcloning of the promoterless cat gene into frequently used plasmids, including those with an ampicillin-resistance marker. In plasmids pCAT10-pCAT40, the cat gene cartridges are flanked by polylinker sequences and can be excised by double digestion with pairs of appropriate restriction enzymes. Translation stop condons in all three reading frames are located upstream from the AUG start codon of the pCAT40 cartridge; the latter can also be excised by a single digestion with the enzymes, SalI, PstI, or HindIII.
Dobrowolski
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Institute of Biotechnology, Leipzig, F.R.G.
D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics*; D003062:Codon / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D007613:Kanamycin Resistance / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*
D003062:Codon; D015500:Chloramphenicol O-Acetyltransferase