Assay of HIV-1 proteinase: a colorimetric method using small peptide substrates.
A colorimetric assay for HIV proteinase using small protected peptide substrates is described. Substrates are cleaved to release N-terminal prolyl peptides which react with isatin to form a blue product which is measured spectrophotometrically. The assay is suitable for use with pure enzyme or crude extracts derived from genetically engineered Escherichia coli.
Broadhurst
A V
AV
Department of Chemotherapy Biology, Roche Products Ltd., Welwyn Garden City, Hertfordshire, United Kingdom.
Roberts
N A
NA
Ritchie
A J
AJ
Handa
B K
BK
Kay
C
C
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology; D016333:HIV Protease / Q000032:analysis*; D007510:Isatin / Q000737:chemistry; D007700:Kinetics; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000032:analysis* / Q000138:chemical synthesis; D011392:Proline / Q000032:analysis; D013379:Substrate Specificity
D009842:Oligopeptides; D007510:Isatin; D011392:Proline; D016333:HIV Protease