Enzyme-linked immunoassays for the detection of Salmonella spp.: a comparison with other methods.
The first enzyme immunoassay for Salmonella was reported in 1977 and since that time several enzyme-linked immuno assays (ELISAs) have been developed, using either polyclonal or monoclonal antibodies that will detect most Salmonella serotypes. Two of these kits have been declared official first status by the Association of Official Analytical Chemists (AOAC). In comparison with a culture method used in collaborative studies, the total assay time is reduced by 2 days and statistical analysis of the data indicated no significant difference. The main problem related to all methods other than traditional culture methods is the occurrence of false-positive and/or false-negative results. False-positive ELISA results can be eliminated by using (combinations of) highly specific monoclonal antibodies. Good enrichment procedures are very important to be sure that the detection limit of approx. 10(5) cells/ml will be reached. In the future even better limits of detection may be achieved by using enzyme amplification or chemiluminescence to decrease the number of false-negative results.
Beumer
R R
RR
Agricultural University, Laboratory of Food Microbiology, Wageningen, The Netherlands.
Brinkman
E
E
Rombouts
F M
FM
D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D005189:False Positive Reactions; D007124:Immunoenzyme Techniques; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012475:Salmonella / Q000302:isolation & purification*; D013997:Time Factors
D011933:Reagent Kits, Diagnostic