High-performance liquid chromatographic assay for the determination of 5-aminosalicylic acid and acetyl-5-aminosalicylic acid concentrations in endoscopic intestinal biopsy in humans.
A high-performance liquid chromatographic method for the simultaneous determination of 5-amino-salicylic acid (5-ASA) and N-acetyl-5-ASA (Ac-5-ASA) concentrations in endoscopic mucosal biopsy homogenates is presented. The mean recoveries of 5-ASA and Ac-5-ASA from spiked blank biopsy homogenates ranged from 95.9 to 120% and from 92.5 to 100%, respectively. The coefficients of variation for 5-ASA and Ac-5-ASA were 0.7-8.6% and 1.4-12.9%, respectively. This method is useful for direct determination of topical availability of 5-ASA and Ac-5-ASA and probably an accurate parameter of drug bioavailability.
De Vos
M
M
University Hospital Ghent, Department of Gastroenterology, Belgium.
Verdievel
H
H
Schoonjans
R
R
Beke
R
R
De Weerdt
G A
GA
Barbier
F
F
D000636:Aminosalicylic Acids / Q000032:analysis*; D001706:Biopsy; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D016099:Endoscopy, Gastrointestinal; D006801:Humans; D007413:Intestinal Mucosa / Q000737:chemistry*; D019804:Mesalamine
D000636:Aminosalicylic Acids; D019804:Mesalamine; C028836:N-acetyl-5-aminosalicylic acid