title: Verapamil and the synthesis of prostacyclin in human veins.
abstract: The synthesis of the vasodilator prostacyclin (PGI2) depends on the intracellular calcium concentration. For this reason the purpose of this study was to document the effects of verapamil (1), a slow calcium channel blocking agent on venous tissue. Human varicose veins were studied which were removed by a stripping operation. The veins were investigated ex vivo and in vitro. For ex vivo studies 11 patients were given 0.01 g 1 i.v. during the operation. In vitro experiments were carried out by an addition of 22 mumol/l 1 to the incubate. The synthesis of PGI2 was studied according to the inhibition of the ADP induced platelet aggregation without and with addition of substrate (prostaglandin endoperoxide H2). 1 did not induce changes in the synthesis of PGI2, neither after administration to patients nor after addition to the incubation fluid compared to control vessels without addition of 1.
authors.affiliation: Martin Luther University Halle-Wittenberg, School of Medicine, Department of Pharmacology and Toxicology.
authors.forename: A
authors.initials: A
authors.lastname: Fahr
authors.forename: R
authors.initials: R
authors.lastname: Langer
authors.forename: W
authors.initials: W
authors.lastname: Sziegoleit
authors.forename: J
authors.initials: J
authors.lastname: Blauschmidt
authors.forename: H
authors.initials: H
authors.lastname: Albert
authors.forename: H
authors.initials: H
authors.lastname: Liebal
authors.forename: H J
authors.initials: HJ
authors.lastname: Mest
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D011464:Epoprostenol / Q000096:biosynthesis*; D006801:Humans; D066298:In Vitro Techniques; D010974:Platelet Aggregation / Q000187:drug effects; D014648:Varicose Veins / Q000378:metabolism; D014680:Veins / Q000187:drug effects / Q000378:metabolism*; D014700:Verapamil / Q000494:pharmacology*
chemical_list: D000244:Adenosine Diphosphate; D014700:Verapamil; D011464:Epoprostenol