Flow cytometric DNA analysis with use of normal lymph node cells as an internal reference standard in ovarian cancer.
Fresh frozen and paraffin-embedded tumor specimens of 21 patients with ovarian cancer were investigated by means of flow cytometry. In 4 patients, the fresh frozen tumor specimens (fresh tumor specimens) of ovarian tumors were measured for the DNA ploidy and index. We used the paraffin-embedded normal lymph node tissue specimens which were acquired from retroperitoneal lymphadenectomy as the internal reference standard (IS). In all 21 patients, the paraffin-embedded tumor specimens mixed with a lymph node specimen as an IS (tumor specimen with IS) were measured. In addition, all 21 paraffin-embedded tumor specimens without a lymph node tissue specimen (tumor specimens without IS) were measured. In 4 cases which were measured with the fresh tumor specimens, all 4 tumor specimens with IS had the same patterns of DNA ploidy as the fresh tumor specimens. Of 21 patients, 6 patients showed different DNA ploidy patterns between the tumor specimens with and without IS. Furthermore, all of these 6 patients showed diploidy in the tumor specimens without IS while showing aneuploidy in the tumor specimens with IS. Fourteen (66.7%) of 21 tumor specimens with IS were aneuploidy despite aneuploidy of only 8 (38.1%) tumor specimens without IS. All 4 patients with stage I had diploidy in the tumor specimens with IS, and 13 (81.3%) of 16 patients with stage III or IV had aneuploidy. All 8 patients with diploidy in the tumor specimens with IS had survived for 1-2 years.(ABSTRACT TRUNCATED AT 250 WORDS)
Takahashi
Y
Y
Department of Obstetrics and Gynecology, Shiga University of Medical Science, Japan.
Chiba
T
T
Matsumoto
H
H
Ishiguro
T
T
Yoshida
Y
Y
D000782:Aneuploidy; D004247:DNA / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008198:Lymph Nodes / Q000166:cytology*; D010051:Ovarian Neoplasms / Q000235:genetics*; D012015:Reference Standards; D012187:Retroperitoneal Space
D004247:DNA