Cloning and sequence analysis of cDNA encoding two crustacean hyperglycemic hormones from the lobster Homarus americanus.
Using the polymerase chain reaction with degenerated oligonucleotides, we have isolated cDNA clones that encode two structurally different (92% identity) crustacean hyperglycemic hormones (CHH) from the lobster Homarus americanus. The deduced amino acid sequences fully agree with previously published data on partial amino acid sequences, amino acid compositions and molecular masses of hyperglycemic peptides in the lobster. A comparative analysis between the deduced primary structure of two lobster CHH and the crab CHH sequence reveals a phylogenetic relationship and allows the prediction of biologically important regions within the structures of these novel neuropeptides.
Tensen
C P
CP
Department of Experimental Zoology, Faculty of Sciences, Catholic University of Nijmegen, The Netherlands.
De Kleijn
D P
DP
Van Herp
F
F
D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins; D001483:Base Sequence; D005075:Biological Evolution; D003386:Brachyura; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry*; D007447:Invertebrate Hormones; D008969:Molecular Sequence Data; D008121:Nephropidae; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009841:Oligonucleotides; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
D060829:Arthropod Proteins; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D009841:Oligonucleotides; C015947:hyperglycemic hormone, crustacean; D004247:DNA