Determination of fendiline in human plasma by means of capillary gas chromatography and nitrogen-phosphorus selective detection.
A sensitive and specific method for the determination of fendiline in human plasma is presented. Fendiline was extracted from human plasma after the addition of phosphate buffer two times with 4 ml of n-hexane. The organic phase was separated and evaporated to dryness at 40 degrees C under a stream of nitrogen. The residue was dissolved and an aliquot was injected into the gas chromatograph. Chromatographic separation was performed with a DB-1 column with helium as carrier gas. Nitrogen-selective detection was performed. Quantification was performed with the signal output. The limit of detection was 1 ng/ml of plasma.
Lohmann
A
A
R & D, Referat Pharmakokinetik, Thiemann Arzneimittel GmbH, Waltrop, Germany.
Diekmann
N
N
D002849:Chromatography, Gas / Q000379:methods* / Q000706:statistics & numerical data; D005275:Fendiline / Q000097:blood*; D006801:Humans; D009584:Nitrogen; D010758:Phosphorus
D010758:Phosphorus; D009584:Nitrogen; D005275:Fendiline