Renal mitochondrial glutathione transport.
Freshly isolated tightly coupled rabbit renal cortical mitochondria rapidly accumulated glutathione (GSH) against an electrical and concentration gradient, and in the presence and absence of pyruvate/malate, succinate, antimycin A, or FCCP. Mitochondrial GSH uptake was dependent on medium GSH concentration, was not saturable, and reached equilibrium within 1 min of addition. Mitochondrial GSH uptake was partially inhibited by glycine, ophthalmic acid, and serine but not glutamate, cysteine, gamma-glutamyl-glutamate, or proline. These results show that 1) mitochondrial GSH uptake is by both a carrier-mediated process and by diffusion, and 2) the GSH carrier system has structural specificity with the glycine residue being a recognition site.
Schnellmann
R G
RG
Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens 30602.
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D004579:Electron Transport; D005978:Glutathione / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008293:Malates / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D011773:Pyruvates / Q000494:pharmacology; D011817:Rabbits; D013386:Succinates / Q000494:pharmacology; D019802:Succinic Acid; D013696:Temperature
D008293:Malates; D011773:Pyruvates; D013386:Succinates; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D000968:Antimycin A; C030298:malic acid; D019802:Succinic Acid; D005978:Glutathione