Relationship between the neural dysgenesis and increased production of class I MHC H-2Kk mRNA and protein in neurons of murine trisomy 16 fetuses.
Neuronal cells from murine trisomy 16 fetuses have increased levels of class I MHC H-2Kk. To determine whether this increased level of H-2Kk protein product resulted from an increased synthesis of mRNA, a 33 base antisense cDNA probe complementary to a region in exon 2 of the H-2Kk sequence (nucleotide 392-424) was synthesized. This probe was used to examine, by in situ hybridization and immunohistochemistry, the neural distribution of H-2Kk mRNA and protein product. A marked elevation of the H-2Kk mRNA and protein were localized in mts16 neuronal populations that were susceptible to dysgenesis. The results implicate the expression of the H-2Kk in the neuropathology of mts16 and its human counterpart, Down syndrome.
Kornguth
S
S
Department of Physiological Chemistry, University of Wisconsin-Madison 53706.
Mack
K J
KJ
Bersu
E
E
D000818:Animals; D001921:Brain / Q000196:embryology / Q000276:immunology*; D002874:Chromosome Mapping; D015342:DNA Probes; D005260:Female; D005333:Fetus; D005865:Gestational Age; D006183:H-2 Antigens / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D014178:Translocation, Genetic; D014314:Trisomy
D015342:DNA Probes; D006183:H-2 Antigens; C024755:H-2K(K) antigen; D015395:Histocompatibility Antigens Class I; D012333:RNA, Messenger