Presence of meprobamate-like molecules in rat neuromuscular junction. Immunohistochemical demonstration at light- and electron-microscopic levels.
The localization of meprobamate-like (MPB-like) molecules in the neuromuscular junction of rats has been investigated at light- and electron- microscopic levels with the peroxidase-antiperoxidase (PAP) immunohistochemical method, using a purified antiserum obtained from rabbits immunized with a meprobamate-bovine serum albumin (MPB-BSA) conjugate. The immunoreaction was found surrounding synaptic vesicles and in protuberant deposits situated in the post-synaptic membrane. These facts suggest the existence of endogenous MPB-like molecules in neuromuscular junction and that the immunostained protuberant deposits should mark the receptors of those molecules.
Tonda
A
A
Departmento de Biología Celular, Facultad de Biología, Universidad Complutense, Madrid, España.
Garcia-Cordovilla
R
R
Martinez-Rodriguez
R
R
D000818:Animals; D007124:Immunoenzyme Techniques; D008297:Male; D008620:Meprobamate / Q000032:analysis*; D008854:Microscopy, Electron; D009469:Neuromuscular Junction / Q000737:chemistry* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013570:Synaptic Membranes / Q000737:chemistry; D013572:Synaptic Vesicles / Q000737:chemistry
D008620:Meprobamate