The external Ca(2+)-dependence of acetylcholine-induced contracture in single innervated and denervated skeletal muscle cells in mice.
We examined morphological differences and compared the Ca(2+)-dependence between acetylcholine (ACh)-contractures in normal and denervated skeletal muscle cells. ACh (1 microM - 1 mM) contracted the normal cells into a jackknife-shape. The higher the concentration of external Ca2+, the greater the ratio of responding cells/total cells observed and the sharper the angles of the "jackknives". ACh contracted the denervated cells into a compression-form, and the contraction was dependent on the external Ca2+. These results indicate that ACh-contractures in both normal and denervated cells are external Ca(2+)-dependent.
Nojima
H
H
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Kimura
I
I
Kimura
M
M
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009119:Muscle Contraction / Q000187:drug effects*; D009121:Muscle Denervation; D009132:Muscles / Q000294:innervation; D010784:Photomicrography
D000109:Acetylcholine; D002118:Calcium