title: The signal peptide of human preproendothelin-1.
abstract: Synthetic mRNAs were produced using either the complete coding sequence of a human preproendothelin-1 cDNA clone or a truncated form in which the portion encoding the first 17 amino acids, representing a putative signal peptide for insertion into the endoplasmic reticulum, was replaced with a methionine codon. The mRNAs were translated in vitro in the presence or in the absence of microsomal membranes. Protection from trypsin digestion demonstrated that the full-length polypeptide, but not the truncated form, could be inserted into the membranes. Sequence analysis revealed that membrane insertion is accompanied by removal of the first 17 amino acids. These results indicate that the first 17 amino acids of human preproendothelin-1 are a functional signal peptide which allows the protein to enter the secretory pathway.
authors.affiliation: Istituto Biosintesi Vegetali, Consiglio Nazionale delle Richerche, Milano, Italy.
authors.forename: M S
authors.initials: MS
authors.lastname: Fabbrini
authors.forename: B
authors.initials: B
authors.lastname: Valsasina
authors.forename: G
authors.initials: G
authors.lastname: Nitti
authors.forename: L
authors.initials: L
authors.lastname: Benatti
authors.forename: A
authors.initials: A
authors.lastname: Vitale
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA; D019332:Endothelin-1; D016232:Endothelins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D021382:Protein Sorting Signals / Q000235:genetics / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism
chemical_list: D019332:Endothelin-1; D016232:Endothelins; D011498:Protein Precursors; D021382:Protein Sorting Signals; D004247:DNA; D014357:Trypsin