(N-succinimidyl 4-pentynoate)hexacarbonyldicobalt: a transition-metal carbonyl complex having similar uses to the Bolton-Hunter reagent.
The synthesis of the transition-metal carbonyl complex (N-succinimidyl 4-pentynoate)hexacarbonyldicobalt [[(C4H4O2N)O(CO)CH2CH2C identical to CH]Co2(CO)6] is described. This cobalt carbonyl complex is structurally similar to the Bolton-Hunter conjugation reagent and has been successfully employed as a nonradioactive tracer for labeling the drug carbamazepine. The metal carbonyl tracer can be detected at extremely low concentrations (ca. 1 pmol) by FT-IR spectroscopy in the v(CO) region (2150-1800 cm-1). The cobalt carbonyl labeled carbamazepine retains good recognition for anti-carbamazepine antibodies. This novel labeling procedure, which can be broadly termed carbonylmetalloimmunoassay (CMIA), has considerable potential for assaying a wide range of biological materials.
Salmain
M
M
Ecole Nationale Supérieure de Chimie de Paris, U.A. C.N.R.S 403, France.
Vessières
A
A
Butler
I S
IS
Jaouen
G
G
D003035:Cobalt / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D013388:Succinimides / Q000737:chemistry*
D009942:Organometallic Compounds; D013388:Succinimides; C069220:(N-succinimidyl 4-pentynoate)hexacarbonyldicobalt; D003035:Cobalt; C022581:Bolton-Hunter reagent