Characterization of seven DA/DAPI-positive bisatellited marker chromosomes by in situ hybridization.
Seven dicentric bisatellited marker chromosomes, ascertained at amniocentesis, chorionic villus sampling, and in blood from an abnormal liveborn were characterized cytogenetically. All seven markers demonstrated brilliant bands by the DA/DAPI technique corresponding to C-band positive regions. Although some dicentric DA/DAPI-positive bisatellited markers have been identified as inverted duplicated 15s, recent literature has suggested that DA/DAPI lacks specificity for chromosome 15. Our evaluation of DA/DAPI-positive bisatellited marker chromosomes by in situ hybridization shows that some originate from chromosome 15 whereas DA/DAPI negative bisatellited markers may not be derived from 15. The morphological variations noted in our studies are discussed with respect to nomenclature.
Plattner
R
R
Department of Medical Genetics, Indiana School of Medicine, Indianapolis 46202.
Heerema
N A
NA
Patil
S R
SR
Howard-Peebles
P N
PN
Palmer
C G
CG
D000649:Amniocentesis; D002675:Child, Preschool; D015193:Chorionic Villi Sampling; D002871:Chromosome Banding; D002884:Chromosomes, Human, Pair 15; D015342:DNA Probes; D004276:DNA, Satellite / Q000032:analysis*; D004214:Distamycins; D005260:Female; D005456:Fluorescent Dyes; D005819:Genetic Markers; D006801:Humans; D007211:Indoles; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D011247:Pregnancy
D015342:DNA Probes; D004276:DNA, Satellite; D004214:Distamycins; D005456:Fluorescent Dyes; D005819:Genetic Markers; D007211:Indoles; C007293:DAPI; C030000:stallimycin