Determination of amidepin in human plasma by reversed-phase high-performance liquid chromatography with fluorescence detection.
An isocratic reversed-phase high-performance liquid chromatographic method for the determination of amidepin has been developed. The method is based on the extraction of alkaline plasma with diethyl ether-dichloromethane, and the injection into the Supelcosil LC-18 column of the evaporated and reconstituted organic phase. After separation, detection is carried out by a fluorescence detector (excitation at 195 nm with no filter). The limit of detection is 10 ng/ml of plasma. The mean coefficient of variation is 12%. The plasma levels after oral administration and after intravenous administration are shown.
Stehlík
P
P
Research Institute for Pharmacy and Biochemistry, Prague, Czechoslovakia.
Houbová
H
H
D000284:Administration, Oral; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003988:Dibenzothiepins / Q000008:administration & dosage / Q000097:blood*; D005453:Fluorescence; D006801:Humans; D007275:Injections, Intravenous; D008790:Metoprolol
D003988:Dibenzothiepins; C056660:amidepine; D008790:Metoprolol