A recombinant clone of Wuchereria bancrofti with DNA specificity for human lymphatic filarial parasites.
In order to understand the immune response to Wuchereria bancrofti and to aid in the diagnosis of W. bancrofti infections, recombinant antigens were identified from a W. bancrofti genomic expression library made in lambda gt11 using a pool of sera from infected Indian patients. One of the recombinant clones, lambda WbN1, containing a 2.5-kb insert, reacted strongly to a pool of sera from patients with lymphatic filariasis but not to normal human sera. In addition, this clone showed restricted specificity at the genomic level to the major lymphatic filarial parasites W. bancrofti and Brugia malayi but not to the closely related filarial parasite Brugia pahangi or to other filarial and non-filarial species tested. Nucleotide sequence analysis indicated the cloned DNA to have homology to myosin-like myofibrillar proteins. Polymerase chain reaction amplification initiated by specific synthetic oligomers amplified DNA in a species-specific manner from as little as 16 pg of isolated DNA or from one microfilaria.
Raghavan
N
N
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
McReynolds
L A
LA
Maina
C V
CV
Feinstone
S M
SM
Jayaraman
K
K
Ottesen
E A
EA
Nutman
T B
TB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D004274:DNA, Recombinant / Q000302:isolation & purification; D004605:Elephantiasis, Filarial / Q000235:genetics / Q000469:parasitology*; D015870:Gene Expression; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D014958:Wuchereria bancrofti / Q000235:genetics* / Q000276:immunology
D004274:DNA, Recombinant; D004247:DNA