Casein kinase I in bovine sperm: purification and characterization.
A highly purified preparation of sperm casein kinase I was obtained by sequential chromatography with phosphocellulose, gel filtration on sephacryl S-300, Affi-gel blue and DEAE-Cellulose. The chromatographic behavior and properties of the enzyme suggest that the sperm enzyme is similar to casein kinase I from other tissues. Antibodies against calf thymus casein kinase I cross-react with the sperm enzyme. A special feature of the sperm enzyme is that the activity is stimulated by spermine.
Chaudhry
P S
PS
Department of Chemistry, New Mexico State University, Las Cruces 88003.
Newcomer
P A
PA
Casillas
E R
ER
D000818:Animals; D047388:Casein Kinases; D002417:Cattle; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D007700:Kinetics; D008297:Male; D009994:Osmolar Concentration; D010766:Phosphorylation; D011494:Protein Kinases / Q000302:isolation & purification* / Q000378:metabolism; D013094:Spermatozoa / Q000201:enzymology*; D013096:Spermine / Q000494:pharmacology; D013379:Substrate Specificity
D013096:Spermine; D011494:Protein Kinases; D047388:Casein Kinases