Structural and functional studies on the human hepatic interleukin-6 receptor. Molecular cloning and overexpression in HepG2 cells.
cDNAs coding for the human hepatic interleukin-6 receptor (IL-6-R) have been isolated from a library made from poly(A) RNA of dexamethasone-treated human hepatoma cells (HepG2). We found the hepatic IL-6-R to be identical to the one expressed by leucocytes. A polyclonal antiserum was raised in rabbits against the IL-6-R protein expressed in Escherichia coli. Although the entire IL-6-R protein was used for immunization, only antibodies to the cytoplasmic domain of the IL-6-R were obtained. It is demonstrated by affinity cross-linking and subsequent immunoprecipitation with antibodies against the ligand as well as against the receptor that the cloned cDNA codes for the functional IL-6-R on HepG2 cells. When the hepatic IL-6-R cDNA was overexpressed in HepG2 cells, these cells became more sensitive to low concentrations of IL-6 with respect to the induction of gamma-fibrinogen mRNA.
Schooltink
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H
Institut für Biochemie der RWTH Aacehn, Federal Republic of Germany.
Stoyan
T
T
Lenz
D
D
Schmitz
H
H
Hirano
T
T
Kishimoto
T
T
Heinrich
P C
PC
Rose-John
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005340:Fibrinogen / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D015850:Interleukin-6 / Q000502:physiology*; D008099:Liver / Q000502:physiology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D011971:Receptors, Immunologic / Q000737:chemistry* / Q000276:immunology; D019947:Receptors, Interleukin-6; D011994:Recombinant Proteins / Q000276:immunology; D015183:Restriction Mapping; D013329:Structure-Activity Relationship
D015850:Interleukin-6; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D019947:Receptors, Interleukin-6; D011994:Recombinant Proteins; D005340:Fibrinogen; D004247:DNA