The porcine tumor necrosis factor-encoding genes: sequence and comparative analysis.
We have cloned and sequenced a 10.22-kb fragment of the genomic locus of the porcine tumor necrosis factor-encoding genes, TNF-alpha and TNF-beta. A liver genomic DNA library, partially digested with Sau3AI, was cloned into the phage lambda EMBL4 and screened with a porcine TNF-alpha cDNA probe. Analysis showed that both the TNF-alpha and TNF-beta genes were present on the cloned fragment. In addition, the cloned fragment contained about 2 kb of repetitive sequences 5' to the TNF-beta gene. The TNF genes are arranged in a tandem repeat, as is the case for the human, mouse and rabbit TNF genes. The comparison of both genes with their human homologues displayed a considerable degree of conservation (80%), suggesting an equal evolution rate.
Kuhnert
P
P
Institute of Veterinary Virology, University of Berne, Switzerland.
Wüthrich
C
C
Peterhans
E
E
Pauli
U
U
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D005091:Exons; D005786:Gene Expression Regulation; D007438:Introns; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D014409:Tumor Necrosis Factor-alpha; D004247:DNA