Molecular cloning and nucleotide sequence of complementary DNA encoding rabbit alpha 1-acid glycoprotein.
Alpha 1-acid glycoprotein (AGP) complementary DNA clone has been isolated from an acute phase rabbit liver library. Complete and full-length nucleotide sequence of this cDNA has been determined. The cDNA contains 35 nucleotides 5' untranslated region followed by 606 nucleotides of coding region and a 112 nucleotides 3' untranslated region. Comparison of this sequence with that of human, rat and mouse reveals a high degree of homology with human alpha 1-AGP. Northern blot analysis of messenger RNA for alpha 1-AGP in normal and acute livers demonstrates remarkable induction of transcription of this gene in rabbit in response to acute inflammation by the administration of turpentine. Such a response of AGP gene expression makes it a major member of the acute phase responsive genes in rabbit liver.
Ray
B K
BK
Department of Veterinary Microbiology, University of Missouri, Columbia 65211.
Ray
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D008099:Liver / Q000502:physiology; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D009961:Orosomucoid / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011817:Rabbits
D015345:Oligonucleotide Probes; D009961:Orosomucoid; D004247:DNA