[Cloning of the Arthrobacter globiformis fcbA gene for dehalogenase and construction of a hybrid pathway of 4-chlorobenzoic acid degradation in Pseudomonas putida].
The Artrobacter globiformis KZT1 fcbA gene responsible for dehalogenase (4-chlorobenzoate-4-hydroxylase) activity was cloned in Escherichia coli and Pseudomonas putida cells. The character of the fcbA gene expression was studied. Notwithstanding amplification of the gene dose and control of the inducible Plac promoter, the level of substrate dehalogenation by recombinant E. coli strains was lower, as compared with that in the original KZT1 strain. Cloning of the fcbA gene in P. putida KZ6R cells utilizing 4HBA resulted in a recombinant pathway of 4CBA degradation, which proved more effective for substrate consumption, in comparison with the original KZT1 strain.
Plotnkova
E G
EG
Tsoĭ
T V
TV
Grishchenkov
V G
VG
Zaĭtsev
G M
GM
Nagaeva
M V
MV
Boronin
A M
AM
D001173:Arthrobacter / Q000235:genetics*; D002723:Chlorobenzoates / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism*; D015870:Gene Expression / Q000502:physiology; D005798:Genes, Bacterial / Q000235:genetics*; D005818:Genetic Engineering; D006867:Hydrolases / Q000235:genetics*; D015394:Molecular Structure; D010957:Plasmids / Q000235:genetics; D011549:Pseudomonas / Q000378:metabolism*; D011995:Recombination, Genetic / Q000502:physiology
D002723:Chlorobenzoates; D006867:Hydrolases; C042846:4-chlorobenzoate dehalogenase; C040768:4-chlorobenzoic acid
Klonirovanie gena degalogenazy fcbA Arthrobacter globiformis i konstruirovanie gibridnogo puti degradatsii 4-khlorbenzoata v Pseudomonas putida.