title: Quantitative analysis of limonin in grapefruit juice using an enzyme-linked immunoassay: interlaboratory study.
abstract: Eleven laboratories participated in an interlaboratory study of an enzyme-linked immunoassay for limonin in grapefruit juice. Participating laboratories received training and practice samples until familiar with the procedure. Laboratories then received 8 sample pairs of grapefruit juice as blind duplicates. Samples were refrigerated and analyzed within 4 days of receipt. Results from 1 laboratory and 6 individual samples were excluded from the statistical analysis. Method performance was measured by the one-way component of variance analysis. Repeatability relative standard deviations (RSDr) ranged from 7.0 to 15.6%. Reproducibility relative standard deviations (RSDR) ranged from 12.9 to 29.4%.
authors.affiliation: Florida Department of Citrus, Lake Alfred 33850.
authors.forename: W W
authors.initials: WW
authors.lastname: Widmer
authors.forename: R L
authors.initials: RL
authors.lastname: Rouseff
mesh_terms: D001628:Beverages / Q000032:analysis*; D002957:Citrus / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D036701:Limonins; D014315:Triterpenes / Q000032:analysis*
chemical_list: D007074:Immunoglobulin G; D007202:Indicators and Reagents; D036701:Limonins; D014315:Triterpenes; C001546:limonin