Distribution of anionic sites in gut tissue: an electron-microscopical study.
A new one-step incubation method using cationic gold colloid was applied to reveal anionic moieties in rat colonic mucosa. Gold particles were detected in all cellular nuclei, basement membranes, mast cell granules and collagen fibres, while the luminal surfaces of all vascular endothelial cells were devoid of gold label. Application of the method for detection of anionic domains under various conditions is discussed.
Dikranian
K
K
Department of Anatomy and Histology, Institute of Medicine, Varna, Bulgaria.
Petrov
M
M
Georgieva
K
K
Burnstock
G
G
D000818:Animals; D000838:Anions; D003106:Colon / Q000378:metabolism* / Q000648:ultrastructure; D006046:Gold; D006651:Histocytochemistry; D007413:Intestinal Mucosa / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D051381:Rats; D011921:Rats, Inbred WKY
D000838:Anions; D006046:Gold