title: [Identification of alpha-tropomyosin of the human myocardium by two-dimensional electrophoresis, and sequencing].
abstract: Two-dimensional electrophoresis of total cardiac muscle extracts allows the detection of about 200 protein fractions. In preliminary studies the fraction D-10 protein was characterized in terms of relative molecular mass, isoelectric point and quantitative composition as alpha-tropomyosin. The similarity of the protein to human alpha-tropomyosin was confirmed by the results of analysis of the N-terminal sequence of the D-10 protein eluate in a gas-phase sequencer.
authors.forename: S S
authors.initials: SS
authors.lastname: Shishkin
authors.forename: Ts A
authors.initials: TsA
authors.lastname: Egorov
authors.forename: L I
authors.initials: LI
authors.lastname: Kovalev
authors.forename: A V
authors.initials: AV
authors.lastname: Laptev
authors.forename: M N
authors.initials: MN
authors.lastname: Tsvetkova
authors.forename: E V
authors.initials: EV
authors.lastname: Puliaeva
authors.forename: S F
authors.initials: SF
authors.lastname: Barbashov
mesh_terms: D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D009206:Myocardium / Q000737:chemistry*; D014335:Tropomyosin / Q000737:chemistry* / Q000235:genetics
chemical_list: D014335:Tropomyosin
vernacular_title: Identifikatsiia alpha-tropomiozina serdechnoĭ myshtsy cheloveka dvumernym élektroforezom i sekvenirovaniem.