title: [Effectiveness of chemical mutagenesis in multiple damage to one or both strands of plasmid DNA].
abstract: Methods for site-directed multiple modification of DNA have been developed and used for modification of either one or two strands of plasmid DNA. Plasmid DNAs modified in the region of the tet gene were transformed into Escherichia coli cells and Tet colonies were screened. It was shown that multiple lesions in one DNA strand performed using either N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or sodium bisulfite were effectively repaired in the cell by error-free mechanism. In contrast, modification of two DNA strands led to induction of mutations. The efficiency of mutagenesis in the case of modification of a local region of one DNA strand with sodium bisulfite and modification of the other strand with MNNG was 1.1-7.9%. Mutations were analysed by restriction mapping and sequencing. All of them were G----A transitions.
authors.forename: T V
authors.initials: TV
authors.lastname: Karamysheva
authors.forename: A V
authors.initials: AV
authors.lastname: Mazin
authors.forename: M K
authors.initials: MK
authors.lastname: Saparbaev
authors.forename: G L
authors.initials: GL
authors.lastname: Dianov
authors.forename: R I
authors.initials: RI
authors.lastname: Salganik
mesh_terms: D004249:DNA Damage / Q000235:genetics*; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D016297:Mutagenesis, Site-Directed / Q000235:genetics*; D009153:Mutagens / Q000633:toxicity*; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics*; D015183:Restriction Mapping; D013447:Sulfites / Q000633:toxicity; D014170:Transformation, Genetic / Q000235:genetics
chemical_list: D009153:Mutagens; D013447:Sulfites; D008769:Methylnitronitrosoguanidine; C009279:sodium bisulfite
vernacular_title: Effektivnost' khimicheskogo mutageneza pri mnozhestvennykh povrezhdeniiakh odnoĭ i dvukh niteĭ plazmidnoĭ DNK.