title: Arginine vasopressin stimulation of chloride secretion in Madin-Darby canine renal epithelial layers.
abstract: Arginine vasopressin (AVP) at a concentration of 0.1 microM stimulates an inward short-circuit current (SCC) in voltage-clamped Madin-Darby canine kidney epithelial layers with a mean peak of 3.1 +/- 0.8 microA cm-2 (n = 6). The response to AVP is markedly attenuated when inward SCC is stimulated with forskolin or prostaglandin E1, in Cl(-)-free media and by the Cl(-)-transport inhibitors, bumetanide and 5-nitro-2-(3-phenylpropylamino)-benzoic acid, consistent with an AVP-stimulated basal to apical Cl- secretion. Endogenous release of prostaglandins may contribute to AVP stimulation of Cl- secretion.
authors.affiliation: Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne.
authors.forename: N L
authors.initials: NL
authors.lastname: Simmons
authors.forename: C D
authors.initials: CD
authors.lastname: Brown
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000527:Alprostadil / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001127:Arginine Vasopressin / Q000494:pharmacology*; D002460:Cell Line; D002712:Chlorides / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology; D004285:Dogs; D004594:Electrophysiology; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000201:enzymology; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D002712:Chlorides; D001127:Arginine Vasopressin; D005576:Colforsin; D000262:Adenylyl Cyclases; D000527:Alprostadil