title: One-step chromogenic equivalent of activated partial thromboplastin time evaluated for clinical application.
abstract: We evaluated the clinical usefulness of a recently developed semi-automated one-step chromogenic equivalent of activated partial thromboplastin time (APTT; Behring). This simple test is easily adaptable for automation. Generally, the results with this chromogenic one-step APTT were at least as precise as those obtained with comparative coagulometric methods. The chromogenic one-step APTT showed, both in vitro and in vivo, adequate sensitivity to congenital intrinsic factor deficiency but no sensitivity to Factor VII deficiency. Unlike a two-step coagulometric APTT (Dade), the one-step chromogenic APTT seemed sensitive to activation products of the contact system, which are present in immunoadsorbed factor-deficient plasma. The in vitro sensitivity of the chromogenic APTT to heparin was comparable with that of a coagulometric APTT, but the sensitivity to heparin in patients' samples differed slightly. The chromogenic APTT is relatively insensitive to anomalies in the fibrinogen-fibrin conversion. Finally, we observed discrepancies between the chromogenic and coagulometric APTT results for plasma of patients with disseminated intravascular coagulation. We conclude that this one-step chromogenic APTT warrants further evaluation for possible use as a routine test for the clinical laboratory.
authors.affiliation: Clinical Laboratories, St. Joseph Hospital, Veldhoven, The Netherlands.
authors.forename: G A
authors.initials: GA
authors.lastname: Ponjee
authors.forename: H L
authors.initials: HL
authors.lastname: Vader
authors.forename: P J
authors.initials: PJ
authors.lastname: de Wild
authors.forename: G W
authors.initials: GW
authors.lastname: Janssen
authors.forename: F
authors.initials: F
authors.lastname: van der Graaf
mesh_terms: D000595:Amino Acid Sequence; D001663:Bilirubin / Q000097:blood; D001779:Blood Coagulation Factors / Q000032:analysis; D002863:Chromogenic Compounds; D005069:Evaluation Studies as Topic; D006454:Hemoglobins / Q000032:analysis; D006493:Heparin / Q000097:blood; D006801:Humans; D008055:Lipids / Q000097:blood; D008969:Molecular Sequence Data; D009842:Oligopeptides; D010314:Partial Thromboplastin Time; D010743:Phospholipids; D012016:Reference Values; D013433:Sulfoglycosphingolipids
chemical_list: D001779:Blood Coagulation Factors; D002863:Chromogenic Compounds; D006454:Hemoglobins; D008055:Lipids; D009842:Oligopeptides; D010743:Phospholipids; D013433:Sulfoglycosphingolipids; D006493:Heparin; D001663:Bilirubin