title: [Rearrangements of yeast chromosomes revealed by pulsed field gel electrophoresis].
abstract: Electrophoretic karyotypes of yeast Saccharomyces cerevisiae integrant strains containing the pYF91 plasmid integrated into the chromosomes I, III, VI, IX, XI were studied. A possibility was demonstrated of visual identification of the chimaeric chromosomes via the molecular weight increase by 13200 bp (the plasmid size) determined by pulsed field gel electrophoresis. Several gamma-rays induced rearrangements of the yeast chimaeric chromosome I causing instability in hybrids were also studied. The deletions induced in the I chromosome were analysed and their size estimated. The technique of pulsed field gel electrophoresis is recommended for determination of insertions and deletions in the chromosomes of yeast Saccharomyces cerevisiae.
authors.forename: B F
authors.initials: BF
authors.lastname: Iarovoĭ
authors.forename: E v
authors.initials: Ev
authors.lastname: Mezhevaia
authors.forename: V L
authors.initials: VL
authors.lastname: Stepanova
authors.forename: I A
authors.initials: IA
authors.lastname: Zakharov
mesh_terms: D002872:Chromosome Deletion; D015825:Chromosomes, Fungal / Q000502:physiology*; D004586:Electrophoresis / Q000379:methods; D015321:Gene Rearrangement / Q000235:genetics*; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000235:genetics*
vernacular_title: Perestroĭki drozhzhevykh khromosom, vyiavliaemye metodom pul'soforeza.