title: [Detection of hybridization probes using antibodies to DNA adducts with cis- and trans-diaminodichloroplatinum (II). I. Immunochemical characteristics of DNA modified by cis- and trans-diaminodichloroplatinum (II)].
abstract: Rabbit antisera elicited against calf thymus DNA modified cis- and trans-diaminedichlorplatinum II (DDP) [Pt/nucleotide ratio - 0.1] contain antibodies specific for Pt-modified DNA immunogen. A specific antibody against DNA-cisDDP and DNA-transDDP was obtained by affinity chromatography. In the ELISA, 3.10(-1 6) mol Pt per micrograms DNA was determined. The epitope structure was determined by using DNA modified with cisDDP and transDDP.
authors.forename: V I
authors.initials: VI
authors.lastname: Kiseleva
authors.forename: O
authors.initials: O
authors.lastname: Vrana
authors.forename: I
authors.initials: I
authors.lastname: Brabets
authors.forename: A M
authors.initials: AM
authors.lastname: Poverennyĭ
mesh_terms: D000818:Animals; D002417:Cattle; D002945:Cisplatin / Q000494:pharmacology*; D004249:DNA Damage; D007150:Immunohistochemistry; D009693:Nucleic Acid Hybridization; D013237:Stereoisomerism; D013950:Thymus Gland / Q000378:metabolism
chemical_list: C034697:transplatin; D002945:Cisplatin
vernacular_title: Detektsiia gibridizatsionnykh zondov s pomoshch'iu antitel k adduktam DNK s tsis- i trans-diamindikhlorplatinoĭ (II). I. Immunokhimicheskaia kharakteristika DNK, modifitsirovannoĭ tsis- i trans-diamindikhlorplatinoĭ (II).