title: c-Ki-ras gene mutations in dysplasia and carcinomas complicating ulcerative colitis.
abstract: One hundred and nine samples comprising carcinomas, adenomas, dysplastic, inflamed and normal mucosa from patients with sporadic colon cancer and ulcerative colitis (UC) were analysed for c-Ki-ras mutations. DNA was extracted from archival paraffin-embedded material, amplified using the polymerase chain reaction (PCR) and the PCR products analysed using restriction enzyme digestion. Forty-two per cent (14/33) of the sporadic carcinoma controls contained Ki-ras codon 12 mutations in contrast to 24% (8/33) of ulcerative colitis carcinomas. A significantly higher c-Ki-ras mutation rate was observed in rectal carcinomas (72%) in comparison to colonic carcinomas (28%) in control patients (P less than 0.04), while the opposite was observed in UC patients. The difference between the incidence of c-Ki-ras mutations in rectal carcinomas in UC (9%) and in sporadic rectal carcinomas (72%) was also significant (P less than 0.01). This lower prevalence rate and different site distribution of c-Ki-ras mutations in UC carcinomas compared to sporadic carcinomas suggests that specific genetic differences may underlie the causation of carcinomas arising in these situations.
authors.affiliation: Department of Pathology, University of Leeds, UK.
authors.forename: S M
authors.initials: SM
authors.lastname: Bell
authors.forename: S A
authors.initials: SA
authors.lastname: Kelly
authors.forename: J A
authors.initials: JA
authors.lastname: Hoyle
authors.forename: F A
authors.initials: FA
authors.lastname: Lewis
authors.forename: G R
authors.initials: GR
authors.lastname: Taylor
authors.forename: H
authors.initials: H
authors.lastname: Thompson
authors.forename: M F
authors.initials: MF
authors.lastname: Dixon
authors.forename: P
authors.initials: P
authors.lastname: Quirke
mesh_terms: D003062:Codon / Q000235:genetics; D003093:Colitis, Ulcerative / Q000150:complications / Q000235:genetics* / Q000473:pathology; D003106:Colon / Q000473:pathology; D003110:Colonic Neoplasms / Q000150:complications / Q000235:genetics* / Q000473:pathology; D004273:DNA, Neoplasm / Q000235:genetics; D011905:Genes, ras; D006801:Humans; D009154:Mutation; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D012004:Rectal Neoplasms / Q000150:complications / Q000235:genetics* / Q000473:pathology; D015183:Restriction Mapping
chemical_list: D003062:Codon; D004273:DNA, Neoplasm