A centrifugation method for the isolation of plasmid DNA without the use of an ultracentrifuge.
This paper describes the use of a high-performance high-speed centrifuge, a Sorvall RC-28S, for the preparation of plasmid DNA using standard CsCl-ethidium bromide gradients. If the sample is layered into the bottom of the gradient, the plasmid DNA can be banded in 24 hours; this is comparable with the time required for ultracentrifuge separations. Alternatively, plasmids can be banded in a fixed-angle rotor in 36 hours when the sample is mixed throughout the gradient solution.
Abeyasekara
G
G
Department of Biology, University of Essex, Colchester, UK.
Rickwood
D
D
D002498:Centrifugation / Q000379:methods*; D002586:Cesium; D002712:Chlorides; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D004996:Ethidium; D010957:Plasmids
D002712:Chlorides; D004269:DNA, Bacterial; D002586:Cesium; D004996:Ethidium; C028019:cesium chloride