Binding of the anticodon domain of tRNA(fMet) to Escherichia coli methionyl-tRNA synthetase.
A stem and loop RNA domain carrying the methionine anticodon (CAU) was designed from the tRNA(fMet) sequence and produced in vitro. This domain makes a complex with methionyl-tRNA synthetase (Kd = 38(+/- 5) microM; 25 degrees C, pH 7.6, 7 mM-MgCl2). The formation of this complex is dependent on the presence of the cognate CAU anticodon sequence. Recognition of this RNA domain is abolished by a methionyl-tRNA synthetase mutation known to alter the binding of tRNA(Met).
Meinnel
T
T
Laboratoire de Biochimie, Unité Associée 240 du Centre National de la Recherche Scientifique Ecole Polytechnique, Palaiseau, France.
Mechulam
Y
Y
Blanquet
S
S
Fayat
G
G
D000926:Anticodon; D001483:Base Sequence; D002151:Calorimetry; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D008718:Methionine-tRNA Ligase / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D012346:RNA, Transfer, Amino Acyl / Q000138:chemical synthesis / Q000235:genetics / Q000378:metabolism*; D012358:RNA, Transfer, Met
D000926:Anticodon; D012346:RNA, Transfer, Amino Acyl; D012358:RNA, Transfer, Met; C024988:tRNA, formylmethionine-; D008718:Methionine-tRNA Ligase