Confocal scanning laser microscopy of mitochondria: a possible tool in the diagnosis of mitochondrial disorders.
This paper describes a non-invasive method for the study of mitochondrial morphology in cultured human skin fibroblasts by confocal scanning laser microscopy after staining the mitochondria with 2-[4-(dimethylaminostyryl]-1-methylpyridinium iodide. This method is applied to compare mitochondria in fibroblasts from healthy individuals and from patients with mitochondrial myopathy. In most cases there is a striking swelling of the patient's mitochondria and a loss of fine structure. The results with respect to the potential of this method as a diagnostic tool are discussed.
Ruiters
M H
MH
Laboratory of Physiological Chemistry, University of Groningen, The Netherlands.
van Spronsen
E A
EA
Skjeldal
O H
OH
Stromme
P
P
Scholte
H R
HR
Pzyrembel
H
H
Smit
G P
GP
Ruitenbeek
W
W
Agsteribbe
E
E
D006801:Humans; D007773:Lactates / Q000097:blood; D007834:Lasers; D008661:Metabolism, Inborn Errors / Q000175:diagnosis*; D008853:Microscopy / Q000295:instrumentation*; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000648:ultrastructure*; D011773:Pyruvates / Q000097:blood
D007773:Lactates; D011773:Pyruvates