Expression of AP 3, 4 and 5 isophytochromes in etiolated oat seedlings (Avena sativa L.).
Type 1 phytochrome from etiolated oat seedlings was digested with V-8 protease. Microsequencing of a 13 kDa fragment yielded a sequence of 31 amino acids. The fragment starting with the alanine residue at position 427 of the entire phytochrome amino acid sequence revealed a heterogeneity (threonine, alanine and asparagine) at position 10. This demonstrates that the phytochrome type A genes AP3, 4 and 5 are expressed as proteins.
Grimm
R
R
Institut für Biologie 2, Freiburg, Germany.
Eckerskorn
C
C
D000595:Amino Acid Sequence; D002523:Edible Grain / Q000235:genetics / Q000528:radiation effects*; D015870:Gene Expression / Q000528:radiation effects; D014644:Genetic Variation / Q000528:radiation effects*; D008027:Light; D008969:Molecular Sequence Data; D010834:Phytochrome / Q000235:genetics*
D010834:Phytochrome