title: The mature MgPa-adhesin of Mycoplasma genitalium.
abstract: A high molecular weight protein of Mycoplasma genitalium (MgPa-protein) was isolated by fractionated solubilization with 1% CHAPS, followed by subsequent extraction with 2% octylglucoside and size exclusion chromatography. The comparison of the N-terminal sequence reported here with published nucleotide sequence data revealed the existence of a signal sequence; the molecular weight of the mature MgPa-protein was calculated to be 153, 134 dalton. The protein shares antigenic determinants with the adhesin of Mycoplasma pneumoniae (P1-protein). Therefore the amino acid sequence of the MgPa-protein was matched to the P1-protein sequence. Five of seven computer predicted hydrophobic regions of both amino acid sequences were located in corresponding regions.
authors.affiliation: Institute for Medical Microbiology and Hygiene, University of Freiburg, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Mader
authors.forename: P C
authors.initials: PC
authors.lastname: Hu
authors.forename: C H
authors.initials: CH
authors.lastname: Huang
authors.forename: E
authors.initials: E
authors.lastname: Schilz
authors.forename: E
authors.initials: E
authors.lastname: Jacobs
mesh_terms: D018829:Adhesins, Bacterial; D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D015153:Blotting, Western; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009174:Mycoplasma / Q000032:analysis*
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; C035471:adhesin, Mycoplasma pneumoniae