title: Secretion of interleukin-1 by bovine milk macrophages.
abstract: The relative sensitivity of bovine blood monocytes and macrophages isolated from milk to lipopolysaccharide, with respect to interleukin 1 (IL-1) production, was evaluated. Addition of lipopolysaccharide (0 to 30 microgram/ml) to the culture medium resulted in increases in secreted and intracellular IL-1 activity for monocytes and milk macrophages, with maximal stimulation achieved at 30 micrograms of lipopolysaccharide/ml of medium. At this concentration of lipopolysaccharide, monocytes released 76% of the total IL-1, whereas milk macrophages released only 26% of the total IL-1 produced within the cell. Secretion of a small quantity of IL-1 was a common property of macrophages isolated from healthy and mastitic quarters. We concluded that limited secretion of IL-1 may render the milk macrophages less efficient in promoting lymphocyte activation.
authors.affiliation: Department of Animal Science, McGill University, Ste Anne de Bellevue, Quebec.
authors.forename: I
authors.initials: I
authors.lastname: Politis
authors.forename: B W
authors.initials: BW
authors.lastname: McBride
authors.forename: J H
authors.initials: JH
authors.lastname: Burton
authors.forename: X
authors.initials: X
authors.lastname: Zhao
authors.forename: J D
authors.initials: JD
authors.lastname: Turner
mesh_terms: D000818:Animals; D002417:Cattle / Q000276:immunology*; D002478:Cells, Cultured; D005260:Female; D007111:Immunity, Cellular; D007375:Interleukin-1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000276:immunology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D008414:Mastitis, Bovine / Q000276:immunology*; D008892:Milk / Q000166:cytology* / Q000276:immunology; D009000:Monocytes / Q000276:immunology
chemical_list: D007375:Interleukin-1; D008070:Lipopolysaccharides