title: The Chlorella H+/hexose cotransporter gene.
abstract: The complete genomic sequence of the inducible Chlorella kessleri H+/hexose cotransporter (HUP1) has been obtained from two overlapping clones isolated from a lambda gt10 library. The HUP1 gene is interrupted by 14 introns with the first intron being located in the 5'-untranslated part of the gene. The average intron length is 220 bp, yielding a very regular intron/exon pattern in the gene. The codon usage in this gene is strongly biased with a clear preference for C and a strong suppression of A. A consensus sequence for a putative algal polyadenylation sequence is shown and compared with other algal cDNA sequences.
authors.affiliation: Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, Federal Republic of Germany.
authors.forename: K
authors.initials: K
authors.lastname: Wolf
authors.forename: W
authors.initials: W
authors.lastname: Tanner
authors.forename: N
authors.initials: N
authors.lastname: Sauer
mesh_terms: D001483:Base Sequence; D002708:Chlorella / Q000235:genetics* / Q000378:metabolism; D003062:Codon / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005796:Genes; D015698:Genomic Library; D007438:Introns; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D010957:Plasmids; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D003062:Codon; D009004:Monosaccharide Transport Proteins; D004247:DNA