title: A new monoclonal antibody to human subcapsular thymic epithelial cells.
abstract: A monoclonal antibody, termed K-20, was generated against an anaplastic thymic carcinoma cell line, Ty-82. Subcapsular thymic epithelial cells of the thymus and blood vessels in various organs were shown to react with the K-20 monoclonal antibody by immunohistochemical staining. Immunofluorescent study revealed that various haematopoietic fresh cells and cell lines did not show any significant reactivity with K-20, except for one Epstein-Barr-virus-carrying lymphoma cell line (SP-50B). Western immunoblotting and affinity purification procedure revealed that K-20 was directed to a protein with a molecular weight of 28 kDa. K-20 is unique in its restrictive reactivity with human subcapsular thymic epithelial cells.
authors.affiliation: Department of Internal Medicine, Kochi Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takeuchi
authors.forename: I
authors.initials: I
authors.lastname: Kubonishi
authors.forename: Y
authors.initials: Y
authors.lastname: Ohtsuki
authors.forename: I
authors.initials: I
authors.lastname: Miyoshi
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000032:analysis*; D002460:Cell Line; D004848:Epithelium / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology; D013953:Thymus Neoplasms / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens