Characterization of human 5S rRNA genes.
The human 5S rRNA genes are found in clusters of tandem repeated units. We have cloned and partially characterized six restriction fragments from two clusters of 2.3 kb and 1.6 kb repeats, respectively. Four fragments from the cluster of 2.3 kb repeats contain a 5S rRNA gene and one fragment contains a gene variant with an additional nucleotide in the internal control region. A fragment from the 1.6 kb cluster contains a gene and is highly homologous to the 2.3 kb repeats, except for a large deletion in the 3'-flanking region starting 12 bp downstream of the gene. The number of genes and closely related gene variants is found to be 300-400 per haploid human genome. 100-150 of these are found in 2.3 kb repeats and 5-10 are found in 1.6 kb repeats. The total number of 5S rRNA sequences, including pseudogenes, is 1700-2000 per haploid genome. The genes and the gene variant are transcribed equally efficient in a HeLa cell extract. If 5'-flanking sequences, including a GC-motif in the -40 region, are removed from the genes, transcription is reduced with a factor 10 or more, suggesting that sequences upstream of the coding region are important for the level of transcription.
Sørensen
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PD
Department of Biochemistry B, University of Copenhagen, Denmark.
Frederiksen
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D001483:Base Sequence; D015139:Blotting, Southern; D002457:Cell Extracts; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D011544:Pseudogenes / Q000235:genetics; D015347:RNA Probes / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000502:physiology
D002457:Cell Extracts; D004275:DNA, Ribosomal; D015347:RNA Probes; D012341:RNA, Ribosomal, 5S