Modulation of elastase-like activity in fibroblasts stimulated with elastin peptides.
Elastin-derived peptides, kappa-elastin, prepared by chemical degradation of insoluble elastin from bovine ligamentum nuchae, were shown to increase the elastase-like activity in the culture medium and cell fractions in fibroblasts. Preincubation of cells with nifedipine (calcium channel blocker) and trifluoperazine (calmodulin antagonist) induced a decrease in the activities of the enzyme under study. These data suggest the possibility of pharmacological modulation of the biological effects induced by elastin-derived peptides.
Gmiński
J
J
Department of Biochemistry and Chemistry, Silesian Medical Academy, Katowice, Poland.
Weglarz
L
L
Drózdz
M
M
Sułkowski
P
P
Goss
M
M
D000818:Animals; D002478:Cells, Cultured; D004549:Elastin / Q000302:isolation & purification / Q000494:pharmacology*; D005347:Fibroblasts / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology; D009543:Nifedipine / Q000494:pharmacology; D010196:Pancreatic Elastase / Q000378:metabolism*; D010446:Peptide Fragments / Q000302:isolation & purification / Q000494:pharmacology*; D014268:Trifluoperazine / Q000494:pharmacology
D010446:Peptide Fragments; D014268:Trifluoperazine; D004549:Elastin; D010196:Pancreatic Elastase; D009543:Nifedipine