Influence of surface texture of polymeric sheets on peripheral nerve regeneration in a two-compartment guidance system.
Synthetic guidance channels are useful tools to study the mechanisms underlying peripheral nerve regeneration. In the present study, the lumen of silicone elastomer tubes was divided into two compartments by a polymer strip 10 mm long placed along the tube length. The influence of varying the surface texture of hydrophilic and hydrophobic polymer strips on the morphology of the regenerated neural tissue was analysed. Hydrophilic nitrocellulose (NC) and hydrophobic polyvinylidene fluoride (PVDF) films with smooth (S-NC and S-PVDF) or rough (R-NC, R-PVDF) surface texture were used. Five channels of each type were used to repair transected rat sciatic nerves and analysed after 4 wk. Tissue strips bridged the nerve stumps in all R-NC and R-PVDF tubes, in five of the S-NC and three of the S-PVDF tubes. In R-NC and R-PVDF tubes, bell-shaped tissue adhering to the polymer strip was observed, whereas in S-NC and S-PVDF tubes round, free-floating nerve cables were seen. All the cables contained myelinated and unmyelinated axons and Schwann cells grouped in microfascicles and surrounded by an epineurial layer. For both rough strips, the initial cell layer consisted of macrophages adhering to the polymer surface. The epineurial nerve tissue contacting the rough surface was significantly thinner for PVDF compared with NC strips. No difference in epineurial thickness was observed for nerves facing the silicone tube or for smooth NC and PVDF strips. S-PVDF tubes contained significantly more myelinated axons than S-NC tubes.(ABSTRACT TRUNCATED AT 250 WORDS)
Guénard
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Section of Artificial Organs, Biomaterials and Cellular Technology, Brown University, Providence, RI 02912.
Valentini
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RF
Aebischer
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D000818:Animals; D001369:Axons / Q000473:pathology; D001672:Biocompatible Materials; D002448:Cell Adhesion; D008297:Male; D009413:Nerve Fibers, Myelinated / Q000473:pathology; D009416:Nerve Regeneration / Q000502:physiology*; D010525:Peripheral Nerves / Q000473:pathology / Q000502:physiology*; D011108:Polymers; D051381:Rats; D011916:Rats, Inbred F344; D012583:Schwann Cells / Q000473:pathology; D012584:Sciatic Nerve / Q000473:pathology / Q000502:physiology; D012826:Silicone Elastomers; D013499:Surface Properties
D001672:Biocompatible Materials; D011108:Polymers; D012826:Silicone Elastomers