A method for enzyme- and immunohistochemical staining of large frozen specimens.
A method for large specimen cryosectioning is described. Specimens of pig heart ventricles were lightly fixed by microwave irradiation, embedded in 10% gelatin, frozen in hexan chilled with dry ice, and sectioned using an LKB 2250 PMW cryomicrotome. The sections were collected on transparent film and transferred onto glass slides. Standard histological, enzyme- and immuno-histochemical staining techniques were used. The present method allowed cryosectioning with sections of good quality which could be used for enzyme- and immuno-staining. For studies of experimental myocardial infarction, staining for phosphorylase and Periodic Acid Schiff outlined the ischemic area and antibodies against plasma fibronectin and fibrinogen delineated the infarcted myocardium.
Holmbom
B
B
Department of Anatomy, University of Umeå, Sweden.
Lindström
M
M
Näslund
U
U
Thornell
L E
LE
D000818:Animals; D005340:Fibrinogen / Q000032:analysis; D005353:Fibronectins / Q000032:analysis; D005629:Frozen Sections; D005780:Gelatin; D006651:Histocytochemistry / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice; D008872:Microwaves; D009203:Myocardial Infarction / Q000378:metabolism*; D009206:Myocardium / Q000737:chemistry*; D013552:Swine
D005353:Fibronectins; D005780:Gelatin; D005340:Fibrinogen