title: Quality of red cell concentrates in relation to the volume of the buffy coat removed by automated processing in a top and bottom system.
abstract: The effect of automated removal of increasing volumes of buffy coat in a 'top and bottom' system on the composition of red cell concentrates (RCC) was investigated. The volume of the buffy coat was adjusted to group 1:50 ml (n = 31), group 2: 70 ml (n = 31) and group 3: 100 ml (n = 31), respectively. The numbers of platelets and leukocytes in the buffy coats were comparable between the groups, whereas the red cell volumes in the buffy coats showed a significant difference (17 +/- 3.6 ml group 1, versus 22 +/- 4.1 ml group 2 and 26 +/- 3.88 ml group 3; p less than 0.001). The volumes, hematocrits and cell counts of the RCC were not significantly different. The plasma volumes were inversely correlated with the volume of buffy coat removed, i.e. 268 +/- 19 ml group 1, versus 257 +/- 15 ml group 2 and 233 +/- 20 ml group 3 (p less than 0.001). We conclude that in the 'top and bottom' system an increase of the volume of the buffy coat from 50 to 100 ml did not improve the quality of the RCC regarding contamination with leukocytes and platelets.
authors.affiliation: Red Cross Blood Bank Amsterdam, The Netherlands.
authors.forename: R N
authors.initials: RN
authors.lastname: Pietersz
authors.forename: W J
authors.initials: WJ
authors.lastname: Dekker
authors.forename: H A
authors.initials: HA
authors.lastname: Reesink
mesh_terms: D001331:Automation; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D004906:Erythrocyte Count; D004912:Erythrocytes; D006801:Humans; D007958:Leukocyte Count; D010976:Platelet Count