A thiostrepton-inducible expression vector for use in Streptomyces spp.
A shuttle expression vector containing the thiostrepton-inducible Streptomyces lividans promoter, ptipA, and the origin of transfer from plasmid RP4 was constructed. Cassettes containing a promoterless xylE gene upstream from a hyg gene were used to demonstrate thiostrepton-inducible expression from ptipA in both S. lividans and Streptomyces ambofaciens, ptipA was estimated to be induced 60-fold or more in Streptomyces ambofaciens.
Kuhstoss
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Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, IN 46285.
Rao
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D000595:Amino Acid Sequence; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005822:Genetic Vectors / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D013302:Streptomyces / Q000235:genetics*; D013883:Thiostrepton / Q000494:pharmacology*
D013883:Thiostrepton