[A comparative radioautographic analysis of DNA synthesis by enterocytes of the small intestine in in vivo and in vitro experiments].
A method of radioautography was used to study DNA synthesis in mice enterocytes as an index of their proliferative activity. Cells were labelled by means of incorporation [3H]thymidine in vivo and in vitro. The program was worked out to computed analysis of the reflected light intensity of obtained radioautographs and estimation of reliability data. Our experiments showed that thymidine diffused very slowly.
stinova
T S
TS
Pal'tsyn
A A
AA
Timin
E N
EN
D000818:Animals; D001345:Autoradiography; D001706:Biopsy; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured / Q000166:cytology / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D013936:Thymidine / Q000008:administration & dosage / Q000378:metabolism; D014316:Tritium / Q000008:administration & dosage
D014316:Tritium; D004247:DNA; D013936:Thymidine
Sravnitel'nyĭ radioavtograficheskiĭ analiz sinteza DNK énterotsitami tonkoĭ kishki v éksperimentakh in vivo i in vitro.