Use of a whole chromosomal probe for identification of Treponema hyodysenteriae.
A whole chromosomal DNA probe labelled with photobiotin was used in a dot blot hybridisation to identify DNA from isolates of Treponema hyodysenteriae, the aetiological agent of swine dysentery. The probe was evaluated using DNA from 13 isolates of T hyodysenteriae and 13 isolates of non-T hyodysenteriae spirochaetes recovered from pigs. The initial test had both a sensitivity and specificity of 92.3 per cent, although when it was repeated the specificity fell to 84.6 per cent. The test was helpful in distinguishing between T hyodysenteriae and other morphologically similar treponemes that are part of the normal flora in the large intestine of pigs. The probe could also be used to detect as little as 10 ng of purified DNA from T hyodysenteriae, or DNA from 2 x 10(6) bacterial cells lysed directly onto nitrocellulose.
Combs
B G
BG
School of Veterinary Studies, Murdoch University, Western Australia.
Hampson
D J
DJ
D000818:Animals; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology*; D014208:Treponema / Q000235:genetics / Q000302:isolation & purification*; D014211:Treponemal Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*
D015342:DNA Probes; D004269:DNA, Bacterial