title: Influence of disodium aurothiomalate on the activities of xanthine dehydrogenase and xanthine oxidase in endothelial cells.
abstract: The effect of aurothiomalate in modulating the conversion of xanthine dehydrogenase to its superoxide producing oxidase form in rat and human liver cytosolic preparations has been investigated. Low concentrations (10(-8)-10(-5) mol.dm-3) of this second-line agent were found to inhibit the conversion of the dehydrogenase to its corresponding oxidase form. High concentrations (10(-4) mol.dm-3), however, accelerated this conversion. It is possible that the influence of aurothiomalate on the relative proportions of xanthine dehydrogenase and xanthine oxidase is a reflection of the gold(I) blockage of critical thiol(ate) or sulphido ligands present in this enzymatic system. These effects may form the basis of aurothiomalate's anti-proliferative action on endothelial cells.
authors.affiliation: Inflammation Group, London Hospital Medical College, UK.
authors.forename: T
authors.initials: T
authors.lastname: Sahinoglu
authors.forename: M
authors.initials: M
authors.lastname: Grootveld
authors.forename: C R
authors.initials: CR
authors.lastname: Stevens
authors.forename: S J
authors.initials: SJ
authors.lastname: Thompson
authors.forename: A W
authors.initials: AW
authors.lastname: Claxson
authors.forename: D R
authors.initials: DR
authors.lastname: Blake
mesh_terms: D000818:Animals; D003600:Cytosol / Q000201:enzymology; D004727:Endothelium / Q000187:drug effects / Q000201:enzymology; D006052:Gold Sodium Thiomalate / Q000494:pharmacology*; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014968:Xanthine Dehydrogenase / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism*
chemical_list: D006052:Gold Sodium Thiomalate; D014968:Xanthine Dehydrogenase; D014969:Xanthine Oxidase