title: Clean-up and determination of sodium valproate in serum by high-performance liquid chromatography with fluorimetric detection.
abstract: A method is reported for the determination of the anticonvulsant sodium valproate in serum by fluorimetric detection. HPLC is performed on a reversed-phase column with a mobile phase of acetonitrile/water (65:35, v/v) with cyclohexane carboxylic acid as an internal standard. Samples were derivatized with 4-bromomethyl-7-methoxycoumarin and the optimized conditions are reported.
authors.affiliation: Istituto di Chimica Farmaceutica e Tossicologica, Facoltá di Farmacia, Università di Catania, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Bousquet
authors.forename: S
authors.initials: S
authors.lastname: Tirendi
authors.forename: N A
authors.initials: NA
authors.lastname: Santagati
authors.forename: M
authors.initials: M
authors.lastname: Salvo
authors.forename: P
authors.initials: P
authors.lastname: Moncada Paterno Castello
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D013050:Spectrometry, Fluorescence; D014635:Valproic Acid / Q000097:blood*
chemical_list: D007202:Indicators and Reagents; D014635:Valproic Acid