In vitro cytotoxicity of Haemophilus influenzae lipopolysaccharides for bovine aortal endothelial cells.
The cytotoxicity of purified lipopolysaccharide (LPS) from a prototype Haemophilus influenzae type b (Hib) strain (Eagan) and three transformants, differing in their LPS phenotype, for bovine aortal endothelial cells (BAOEC) was investigated. All LPS preparations caused cell disruption and release of lactate dehydrogenase (LDH), an indicator of cytotoxicity, from BAOEC monolayers but to differing extents. There was no correlation between the cytotoxicity of purified Hib LPS to BAOEC monolayers and potential to cause bacteraemia in experimental animals.
Langford
P R
PR
Department of Paediatrics, John Radcliffe Hospital, Oxford, U.K.
Szabo
M
M
Moxon
E R
ER
D000818:Animals; D001011:Aorta; D002417:Cattle; D002470:Cell Survival; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D006193:Haemophilus influenzae; D008070:Lipopolysaccharides / Q000633:toxicity*
D008070:Lipopolysaccharides