title: Amphibian prolactins: activity in the eft skin transepithelial potential bioassay.
abstract: The effects of purified prolactins isolated from frogs (fPRL; Rana catesbeiana) and newts (nPRL; Cynops pyrrhogaster) were compared with those of ovine prolactin (oPRL) and thyrotropin releasing hormone (TRH) in the eft (Notophthalmus viridescens) skin transepithelial potential (TEP) bioassay. At total doses as low as 0.4 micrograms/animal, both fPRL and nPRL were as effective as oPRL in reducing eft skin TEP. By contrast, TRH at total doses as high as 400 micrograms/animal was ineffective in altering eft skin TEP. These data provide the first direct evidence that amphibian PRLs can exert significant physiological control over salamandrid integumental sodium transport.
authors.affiliation: Department of Biological Sciences, State University of New York, Albany 12222.
authors.forename: S C
authors.initials: SC
authors.lastname: Brown
authors.forename: P S
authors.initials: PS
authors.lastname: Brown
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Matsuda
authors.forename: S
authors.initials: S
authors.lastname: Kikuyama
mesh_terms: D000818:Animals; D001681:Biological Assay; D004594:Electrophysiology; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D009673:Notophthalmus viridescens; D010903:Pituitary Gland, Anterior / Q000737:chemistry; D011388:Prolactin / Q000302:isolation & purification / Q000494:pharmacology*; D011892:Rana catesbeiana; D012455:Salamandridae; D012756:Sheep; D012867:Skin / Q000187:drug effects; D012879:Skin Physiological Phenomena; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology
chemical_list: D013973:Thyrotropin-Releasing Hormone; D011388:Prolactin