Expression of HIV-1 in the cerebrospinal fluid detected by the polymerase chain reaction and its correlation with central nervous system disease.
The polymerase chain reaction (PCR) was used to detect HIV-1 sequences (gag, pol, and env) in the cerebrospinal fluid (CSF) and serum samples from 53 HIV-1-positive patients and the results correlated with clinical evidence of neurological disease. Twenty-three out of 24 patients with neurological disease had HIV-1-specific sequences in CSF compared with four out of 20 asymptomatic patients who had no evidence of neurological involvement. The detection of HIV RNA sequences by PCR in the CSF of HIV-positive patients may provide early, rapid and direct evidence of neurological involvement in asymptomatic subjects.
Goswami
K K
KK
Department of Medical Microbiology, University College and Middlesex School of Medicine, London, UK.
Miller
R F
RF
Harrison
M J
MJ
Hamel
D J
DJ
Daniels
R S
RS
Tedder
R S
RS
D000328:Adult; D001483:Base Sequence; D015331:Cohort Studies; D003854:Deoxyribonucleotides; D005260:Female; D006679:HIV Seropositivity / Q000134:cerebrospinal fluid* / Q000150:complications / Q000453:epidemiology; D015497:HIV-1 / Q000302:isolation & purification*; D006716:Homosexuality; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid* / Q000150:complications; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D012367:RNA, Viral / Q000134:cerebrospinal fluid*
D003854:Deoxyribonucleotides; D012367:RNA, Viral