Duodenal rapeseed oil infusion in early and midlactation cows. 5. Milk fatty acids and adipose tissue lipogenic activities.
Lipogenic activities of perirenal adipose tissue were investigated in early (wk 3) and midlactation (wk 19 to 26) cows that received a duodenal rapeseed oil infusion (1.0 to 1.1 kg/d). In midlactation, oil infusion resulted in a decreased rate of fatty acid synthesis from acetate and a decreased rate of the activities of fatty acid synthetase and glucose-6-phosphate dehydrogenase, whereas lipoprotein lipase activity tended to increase. The rate of glucose incorporation into glyceride-glycerol and the activities of glycerol-3-phosphate dehydrogenase and malic enzyme were not significantly affected. Fatty acid C14:0 content of perirenal adipose tissue was decreased, and fatty acid C18:2 and C18:3 contents were increased in oil-infused cows. In early lactation, rates of acetate incorporation into fatty acids and activities of fatty acid synthetase and lipoprotein lipase were very low. Activities of glucose-6-phosphate dehydrogenase and glycerol-3-phosphate dehydrogenase were lower in the early than in the midlactation trial. Oil infusion did not change the measured parameters. In both trials, percentages and yields of milk fatty acids C18:1, C18:2, and C18:3 were increased, whereas those of C14:0 and C16:0 were decreased by oil. Calculated transfer rates of absorbed fatty acid C18:2 from oil to milk fat were 16 to 26%. Results suggested that oil fatty acids affected adipose and mammary de novo lipogenesis in a direct way without affecting fatty acid esterification in adipose tissue or total fat secretion in mammary tissue.
Chilliard
Y
Y
Laboratoire de la Lactation, Institut National de la Recherche Agronomique, Saint-Genès Champanelle, France.
Gagliostro
G
G
Flechet
J
J
Lefaivre
J
J
Sebastian
I
I
D000085:Acetates / Q000378:metabolism; D000273:Adipose Tissue / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D004386:Duodenum; D004734:Energy Metabolism; D064429:Fatty Acid Synthases / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D005229:Fatty Acids, Monounsaturated; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D007774:Lactation / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis*; D008071:Lipoprotein Lipase / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D008892:Milk / Q000737:chemistry; D010938:Plant Oils / Q000008:administration & dosage*; D000074262:Rapeseed Oil
D000085:Acetates; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D008055:Lipids; D010938:Plant Oils; D000074262:Rapeseed Oil; D005993:Glycerolphosphate Dehydrogenase; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D064429:Fatty Acid Synthases; D008071:Lipoprotein Lipase