title: Isolation of two antigens from the culture filtrates of Mycobacterium tuberculosis and their applications in the laboratory diagnosis of the tuberculous meningitis.
abstract: Two antigens were isolated from the culture filtrates of H37Ra Mycobacterium tuberculosis by immunoabsorbent affinity chromatography, M. tuberculosis antigen 5 and immunoabsorbent affinity column-purified antigen (IAP). The potential application of these two mycobacterial antigens in the laboratory diagnosis of tuberculous meningitis was evaluated by indirect enzyme-linked immunoabsorbent assay in cerebrospinal fluid specimens. IAP antigen was more sensitive than antigen 5, although antigen 5 was more specific than IAP antigen in detecting tuberculous aetiology. Technical aspects of immunoabsorbent affinity chromatography have been highlighted in this study.
authors.affiliation: Department of Pathology Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
authors.forename: V V
authors.initials: VV
authors.lastname: Krishnan
authors.forename: A
authors.initials: A
authors.lastname: Mathai
mesh_terms: D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007122:Immunoelectrophoresis; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D014390:Tuberculosis, Meningeal / Q000175:diagnosis*
chemical_list: D000942:Antigens, Bacterial