title: [The homogeneity of a population of production strain 205 of the tick-borne encephalitis virus].
abstract: Tick-borne encephalitis virus strain 205 was characterized by such sign as the presence of negative colonies. In the swine fibroblast cell line the population of the strain was represented mainly by large plaque (67.3%). Medium-sized plaques prevailed in chick embryo fibroblast cell culture. Protamine sulfate and magnesium chloride reduced the period of incubation, increased the resolving capacity of the plaque method and the proportion of medium- and small-sized plaques. Agarose B rendered the virus colonies less contrasting. The production strain and the clones of its population were found to have pronounced reproductive and protective properties. The S+ variants of the virus exhibited the highest reproductiveness.
authors.forename: I V
authors.initials: IV
authors.lastname: Kotykhov
authors.forename: I P
authors.initials: IP
authors.lastname: Mal'chikova
authors.forename: N N
authors.initials: NN
authors.lastname: Kiseleva
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Fedorov
mesh_terms: D000818:Animals; D004669:Encephalitis Viruses, Tick-Borne / Q000187:drug effects / Q000254:growth & development* / Q000276:immunology; D015636:Magnesium Chloride / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011479:Protamines / Q000494:pharmacology; D012692:Serial Passage; D015164:Vaccines, Inactivated; D010948:Viral Plaque Assay; D014765:Viral Vaccines; D014776:Virus Cultivation
chemical_list: D011479:Protamines; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D015636:Magnesium Chloride
vernacular_title: Odnorodnost' populiatsii proizvodstvennogo shtamma 205 virusa kleshchevogo éntsefalita.