Presynaptic localization of omega-conotoxin-sensitive calcium channels at the frog neuromuscular junction.
By the use of anti-omega-CTx antibodies in indirect immunofluorescence we demonstrated the presence of omega-CTx binding sites in the presynaptic compartment of frog nerve-muscle preparations. The images we obtained indicate that omega-CTx-sensitive channels are clustered at discrete sites corresponding in distribution to active zones.
Torri Tarelli
F
F
Department of Medical Pharmacology, Bruno Ceccarelli Center for the Study of Peripheral Neuropathies and Neuromuscular Diseases, Milan, Italy.
Passafaro
M
M
Clementi
F
F
Sher
E
E
D000818:Animals; D001001:Anura; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D066298:In Vitro Techniques; D009469:Neuromuscular Junction / Q000737:chemistry / Q000187:drug effects*; D010456:Peptides, Cyclic / Q000494:pharmacology*; D020960:omega-Conotoxins
D002121:Calcium Channel Blockers; D010456:Peptides, Cyclic; D020960:omega-Conotoxins; C053423:Conus magus toxin