title: Escherichia coli orfE (upstream of pyrE) encodes RNase PH.
abstract: RNase PH from extracts of Escherichia coli was purified to homogeneity and subjected to NH2-terminal sequencing. Comparison of this sequence with all open reading frames in the GenBank data base revealed at least 95% identity to an unidentified open reading frame (orfE) upstream of pyrE at 81.7 min on the E. coli chromosome. Clones of orfE overexpress RNase PH activity, verifying that orfE encodes this ribonuclease. We suggest that orfE be renamed rph.
authors.affiliation: Department of Biochemistry, University of Connecuticut Health Center, Farmington 06030.
authors.forename: K A
authors.initials: KA
authors.lastname: Ost
authors.forename: M P
authors.initials: MP
authors.lastname: Deutscher
mesh_terms: D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005095:Exoribonucleases / Q000235:genetics* / Q000302:isolation & purification; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids
chemical_list: C056502:ribonuclease PH; D005095:Exoribonucleases