Clean-up and determination of sodium valproate in serum by high-performance liquid chromatography with fluorimetric detection.
A method is reported for the determination of the anticonvulsant sodium valproate in serum by fluorimetric detection. HPLC is performed on a reversed-phase column with a mobile phase of acetonitrile/water (65:35, v/v) with cyclohexane carboxylic acid as an internal standard. Samples were derivatized with 4-bromomethyl-7-methoxycoumarin and the optimized conditions are reported.
Bousquet
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Istituto di Chimica Farmaceutica e Tossicologica, Facoltá di Farmacia, Università di Catania, Italy.
Tirendi
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Santagati
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Salvo
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Moncada Paterno Castello
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P
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D013050:Spectrometry, Fluorescence; D014635:Valproic Acid / Q000097:blood*
D007202:Indicators and Reagents; D014635:Valproic Acid