title: Plasmid incidence rate and conjugative chloramphenicol and tetracycline resistance plasmids in Malaysian isolates of Salmonella typhi.
abstract: Seven (6.1%) of 115 strains of Salmonella typhi isolated from Malaysian patients harbored a single large plasmid of 71 to 166 mD. Two of the seven plasmid-bearing strains were resistant to chloramphenicol (Cm) and tetracycline (Tc) and they transferred Cm and Tc resistance traits to Escherichia coli K12 at frequencies from 1.6 x 10(-7) to 1.9 x 10(-6). Agarose gel electrophoresis provided evidence that the resistance traits were cotransferred on a conjugative plasmid. The significance and importance of these results are discussed.
authors.affiliation: Institute for Advanced Studies, University of Malaya, Kuala Lumpur.
authors.forename: M
authors.initials: M
authors.lastname: Phipps
authors.forename: T
authors.initials: T
authors.lastname: Pang
authors.forename: C L
authors.initials: CL
authors.lastname: Koh
authors.forename: S
authors.initials: S
authors.lastname: Puthucheary
mesh_terms: D002702:Chloramphenicol Resistance / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008296:Malaysia; D010957:Plasmids; D012485:Salmonella typhi / Q000235:genetics*; D013753:Tetracycline Resistance / Q000235:genetics*