Immunogold labelling of the intermediate filament-lamina-nuclear matrix system in HeLa and BHK-21 cells.
Whole-mount, sequentially extracted cells combined with immunogold electron microscopy were developed to demonstrate the intermediate filaments, lamina, and nuclear matrix (IF-L-NM) and to identify their protein components. The IFs of HeLa cells were reacted both with keratin and vimentin monoclonal antibodies; meanwhile, the IF network of BHK-21 cell was reacted only with vimentin monoclonal antibody. The lamina and nuclear matrices of both HeLa and BHK-21 cell were labelled, respectively, with lamin monoclonal antibody-gold complex and 280 Kd nuclear matrix protein monoclonal antibody-gold complex. The monoclonal antibody to keratin could cross-react with the lamina both of HeLa and BHK-21 cells.
Jiao
R J
RJ
Department of Biology, Peking University, Beijing, People's Republic of China.
Wu
D L
DL
Zhang
B
B
Cai
S T
ST
Zhai
Z H
ZH
D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure*; D002478:Cells, Cultured; D006224:Cricetinae; D006046:Gold; D006367:HeLa Cells; D006801:Humans; D007382:Intermediate Filaments / Q000648:ultrastructure*; D016253:Microscopy, Immunoelectron / Q000379:methods*; D015530:Nuclear Matrix / Q000648:ultrastructure*
D006046:Gold