Protective immunity induced in Aotus monkeys by recombinant SERA proteins of Plasmodium falciparum.
We describe the vaccination of Panamanian monkeys (Aotus sp.) with two recombinant blood stage antigens that each contain a portion of the N-terminal region of the SERA (serine repeat antigen) protein of the malaria parasite Plasmodium falciparum. We immunized with either a 262-amino-acid SERA fragment (SERA I) that contains amino acids 24 to 285 of the 989-amino-acid protein or a 483-amino-acid SERA fragment (SERA N) that contains amino acids 24 to 506 as part of a fusion protein with human gamma interferon. The recombinant proteins were shown to stimulate protective immunity when administered with complete and incomplete Freund adjuvant. Four of six immunized monkeys challenged by intravenous inoculation with blood stage P. falciparum developed parasitemias that were reduced by at least 1,000-fold. Two of six immunized monkeys developed parasitemias which were comparable to the lowest parasitemia in one of four controls and were 50- to 1,000-fold lower than in the other three controls.
Inselburg
J
J
Dartmouth Medical School, Hanover, New Hampshire 03756.
Bzik
D J
DJ
Li
W B
WB
Green
K M
KM
Kansopon
J
J
Hahm
B K
BK
Bathurst
I C
IC
Barr
P J
PJ
Rossan
R N
RN
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D000953:Antigens, Protozoan / Q000276:immunology*; D001029:Aotus trivirgatus; D005620:Freund's Adjuvant / Q000276:immunology; D007114:Immunization; D007371:Interferon-gamma / Q000276:immunology; D008288:Malaria / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D010963:Plasmodium falciparum / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D010446:Peptide Fragments; D011994:Recombinant Proteins; D007371:Interferon-gamma; D005620:Freund's Adjuvant