title: Two-dimensional crystals of streptavidin on biotinylated lipid layers and their interactions with biotinylated macromolecules.
abstract: Streptavidin forms two-dimensional crystals when specifically bound to layers of biotinylated lipids at the air/water interface. The three-dimensional structure of streptavidin determined from the crystals by electron crystallography corresponds well with the structure determined by x-ray crystallography. Comparison of the electron and x-ray crystallographic structures reveals the occurrence of free biotin-binding sites on the surface of the two-dimensional crystals facing the aqueous solution. The free biotin-binding sites could be specifically labeled with biotinylated ferritin. The streptavidin/biotinylated lipid system may provide a general approach for the formation of two-dimensional crystals of biotinylated macromolecules.
authors.affiliation: Department of Cell Biology, Stanford University School of Medicine, California 94305.
authors.forename: S A
authors.initials: SA
authors.lastname: Darst
authors.forename: M
authors.initials: M
authors.lastname: Ahlers
authors.forename: P H
authors.initials: PH
authors.lastname: Meller
authors.forename: E W
authors.initials: EW
authors.lastname: Kubalek
authors.forename: R
authors.initials: R
authors.lastname: Blankenburg
authors.forename: H O
authors.initials: HO
authors.lastname: Ribi
authors.forename: H
authors.initials: H
authors.lastname: Ringsdorf
authors.forename: R D
authors.initials: RD
authors.lastname: Kornberg
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D001665:Binding Sites; D001710:Biotin / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008081:Liposomes; D008854:Microscopy, Electron; D008961:Models, Structural; D008968:Molecular Conformation; D011485:Protein Binding; D011487:Protein Conformation; D019809:Streptavidin; D013861:Thiocyanates
chemical_list: D001426:Bacterial Proteins; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008081:Liposomes; D013861:Thiocyanates; D001710:Biotin; D019809:Streptavidin; D016650:Fluorescein-5-isothiocyanate