title: Characterization of 20 Entamoeba histolytica strains isolated from patients with HIV infection.
abstract: Twenty Entamoeba histolytica strains from patients with HIV-1 infection were isolated and compared with E. histolytica strains from patients without HIV infection. The isoenzyme pattern of the hexokinase as well as the hybridization with known DNA-probes were used as markers for pathogenicity. According to these markers, all 20 strains could be regarded as being nonpathogenic. Direct measurements of the virulence were carried out: destruction of monolayer tissue culture cells, capacity of phagocytosis and the ability to induce liver abscesses in the hamster. The virulence of strains from HIV patients was comparable to that of E. histolytica strains which had been isolated from HIV-negative asymptomatic carriers. In agreement with this, none of the HIV-positive patients showed symptoms of an invasive amebiasis. By PRC, no HIV-1 proviral DNA could be evidenced in the E. histolytica strains which had been isolated from HIV patients.
authors.affiliation: Bernhard-Nocht-Institut für Tropenmedizin, Klin. Abteilung, Hamburg, Germany.
authors.forename: G D
authors.initials: GD
authors.lastname: Burchard
authors.forename: F T
authors.initials: FT
authors.lastname: Hufert
authors.forename: D
authors.initials: D
authors.lastname: Mirelman
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D000818:Animals; D015342:DNA Probes / Q000235:genetics; D004748:Entamoeba histolytica / Q000201:enzymology / Q000302:isolation & purification* / Q000472:pathogenicity; D004749:Entamoebiasis / Q000150:complications; D015497:HIV-1; D006593:Hexokinase / Q000032:analysis; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D009693:Nucleic Acid Hybridization / Q000235:genetics; D013045:Species Specificity; D014774:Virulence
chemical_list: D015342:DNA Probes; D007527:Isoenzymes; D006593:Hexokinase