title: Rapid preparation of lysate for immunoprecipitation using a low protein-binding micro-filter.
abstract: A simple method to prepare cell lysate for immunoprecipitation is described. The procedure utilizes filtration of cell lysates by using a low protein-binding filter. This filtration method gave an equivalent result to that of the centrifugation method.
authors.affiliation: Center for Biotechnology, Baylor College of Medicine, The Woodlands, Texas 77381.
authors.forename: M
authors.initials: M
authors.lastname: Ayres
authors.forename: H B
authors.initials: HB
authors.lastname: Marshall
authors.forename: J
authors.initials: J
authors.lastname: Ishikawa
authors.forename: R
authors.initials: R
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D002498:Centrifugation; D004591:Electrophoresis, Polyacrylamide Gel; D005374:Filtration; D011233:Precipitin Tests / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal