title: Activation of the prophenoloxidase cascade and initiation of nodule formation in locusts by bacterial lipopolysaccharides.
abstract: The activation of the prophenoloxidase (proPO) system of the locusts, Schistocerca gregaria and Locusta migratoria, by several bacterial lipopolysaccharides (LPS) is described. Activation of proPO by LPS occurred only in the presence of whole blood homogenates and not with hemocyte lysate preparations alone. Levels of phenoloxidase generated by the different LPSs in vitro were also correlated with numbers of nodules formed in vivo by injection of these LPSs. This further strengthens the evidence for the involvement of proPO activation in the insect cellular defenses. Finally, the wisdom in using anticoagulants in order to stabilize fragile hemocytes in studies on the proPO system is discussed.
authors.affiliation: Biomedical and Physiological Research Group, School of Biological Sciences, University College of Swansea, Wales, UK.
authors.forename: N A
authors.initials: NA
authors.lastname: Ratcliffe
authors.forename: J L
authors.initials: JL
authors.lastname: Brookman
authors.forename: A F
authors.initials: AF
authors.lastname: Rowley
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D001419:Bacteria / Q000032:analysis; D004156:Catechol Oxidase / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D004792:Enzyme Precursors / Q000378:metabolism*; D005549:Foreign-Body Reaction / Q000503:physiopathology; D006110:Grasshoppers / Q000276:immunology* / Q000378:metabolism; D006434:Hemocytes / Q000187:drug effects / Q000201:enzymology*; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000925:Anticoagulants; D004792:Enzyme Precursors; D008070:Lipopolysaccharides; C038451:pro-phenoloxidase; D004156:Catechol Oxidase