Production and characterization of a human hyperimmune intravenous immunoglobulin against Pseudomonas aeruginosa and Klebsiella species.
A hyperimmune globulin for intravenous use (H-IVIG) was prepared from the plasma of donors immunized with Pseudomonas aeruginosa and Klebsiella vaccines. H-IVIG preparations contained substantially higher IgG antibody levels to all nine P. aeruginosa vaccine antigens and to 22 of the 24 Klebsiella vaccine antigens than did commercial IVIG. The H-IVIG was more effective at promoting the opsonophagocytic killing of P. aeruginosa and Klebsiella vaccine serotype strains than normal IVIG. The H-IVIG neutralized greater than 20 times more toxin A than commercial IVIG. Only the H-IVIG offered significant protection against Klebsiella K2 sepsis. The H-IVIG provided significantly better protection against six of the eight P. aeruginosa vaccine serotypes than normal IVIG when compared in a murine burn wound sepsis model. The H-IVIG also protected mice against an Enterobacter aerogenes challenge, whereas normal IVIG was ineffective.
Cryz
S J
SJ
Swiss Serum and Vaccine Institute, Bern.
Fürer
E
E
Sadoff
J C
JC
Fredeking
T
T
Que
J U
JU
Cross
A S
AS
D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D004754:Enterobacter; D004756:Enterobacteriaceae Infections / Q000517:prevention & control; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification; D007136:Immunoglobulins / Q000276:immunology* / Q000302:isolation & purification; D016756:Immunoglobulins, Intravenous; D007709:Klebsiella / Q000276:immunology*; D007710:Klebsiella Infections / Q000517:prevention & control; D051379:Mice; D009895:Opsonin Proteins / Q000276:immunology; D010587:Phagocytosis; D011552:Pseudomonas Infections / Q000517:prevention & control; D011550:Pseudomonas aeruginosa / Q000276:immunology*
D001428:Bacterial Vaccines; D007106:Immune Sera; D007074:Immunoglobulin G; D007136:Immunoglobulins; D016756:Immunoglobulins, Intravenous; D009895:Opsonin Proteins