title: Voltage-dependent inactivation of the potassium current of embryonic chick hepatocytes.
abstract: The whole-cell patch electrode voltage clamp technique was used to study the inactivation properties of the delayed rectifying potassium current of single cultured embryonic chick hepatocytes at 20 degrees C. The potassium current activates maximally within 250-500 ms of membrane depolarization, after which it decays with a monoexponential time course. Both steady-state activation and inactivation are voltage dependent. Steady-state inactivation declines from 100% at -5 mV to 0 near -70 mV. with half inactivation at -41 mV. At the resting potential (EM) of these cells (-21.5 +/- 6.0 mV, n = 36) 6-18% of the IK channels are not inactivated and less than 5% are open. Development and removal of inactivation follow single exponential time courses. The inactivation time constant attains a maximum of around 30 s at -35 mV and is sharply voltage dependent at the EM of these cells. Measurement of EM under current clamp shows random oscillations of 5-10 mV amplitude. We suggest that the voltage- and time-dependent properties of IK, in tandem with a time- and voltage-independent, non-selective current also seen here, would provide the mechanism for a fluctuating EM.
authors.affiliation: Department of Biology, Concordia University, Montréal, Qué, Canada.
authors.forename: C E
authors.initials: CE
authors.lastname: Hill
authors.forename: A
authors.initials: A
authors.lastname: Shrier
mesh_terms: D000818:Animals; D002642:Chick Embryo; D004594:Electrophysiology; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008564:Membrane Potentials / Q000502:physiology; D015221:Potassium Channels / Q000502:physiology*
chemical_list: D015221:Potassium Channels