Liposome-encapsulated antibiotics: preparation, drug release and antimicrobial activity against Pseudomonas aeruginosa.
Ticarcillin- and tobramycin-resistant strains of Pseudomonas aeruginosa were shown to have a markedly increased sensitivity to antibiotics enclosed in liposomes. This was demonstrated by growth inhibition of two resistant P. aeruginosa strains in the presence of the liposome-enclosed ticarcillin and tobramycin at 2 per cent and 20 per cent of their respective minimum inhibitory concentration. The liposome-enclosed antibiotic was as effective against the beta-lactamase-producing strain as against the non-beta-lactamase producing strain. Entrapment efficiency of the two antibiotics with the dehydration-rehydration vesicle (DRV) method was largely superior to other methodologies used. Kinetic studies with DRV demonstrated that tobramycin and ticarcillin-loaded liposomes still contained 83 per cent and 67 per cent of drug respectively after 24 h at 37 degrees C.
Lagacé
J
J
Department of Microbiology and Immunology, Faculty of Medicine, University of Montreal, Quebec, Canada.
Dubreuil
M
M
Montplaisir
S
S
D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004337:Drug Carriers; D004339:Drug Compounding; D004352:Drug Resistance, Microbial; D004355:Drug Stability; D008081:Liposomes; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D013982:Ticarcillin / Q000008:administration & dosage / Q000494:pharmacology; D014031:Tobramycin / Q000008:administration & dosage / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D008081:Liposomes; D013982:Ticarcillin; D014031:Tobramycin