Diverse forms of stress result in changes in cellular levels of osteonectin/SPARC without altering mRNA levels in osteoligament cells.
The osteonectin/SPARC gene has been shown to possess motifs for a heat shock element and metal responsiveness. Also, the expression of the protein has been associated with culture stress in endothelial cells. In the present study, osteoligament (OL) cells derived from the patellar ligament were subjected to diverse forms of stress that included (a) exposure to sodium arsenite, (b) heat shock, (c) cadmium ion, and (d) the amino acid analog, AZC. Osteonectin/SPARC levels in OL cells were determined by Western blot analyses, and immunoprecipitation using antiosteonectin antibodies. Expression of osteonectin/SPARC mRNA was determined by Northern analysis using a 1.5 kb EcoRI restriction fragment of bovine osteonectin cDNA. These studies reveal that osteonectin/SPARC is produced following diverse forms of stress, however, the levels are lower than observed in unchallenged OL cells. In all instances, the mRNA levels were comparable to control cells. These studies indicate that expression of osteonectin/SPARC mRNA is tightly controlled in OL cells and that the protein may be regulated at the level of protein translation.
Sauk
J J
JJ
Department of Oral Pathology, University of Maryland Dental School, Baltimore 21201.
Norris
K
K
Kerr
J M
JM
Somerman
M J
MJ
Young
M F
MF
D000818:Animals; D001151:Arsenic / Q000494:pharmacology; D018053:Arsenites; D001383:Azetidinecarboxylic Acid / Q000494:pharmacology; D001483:Base Sequence; D002104:Cadmium / Q000494:pharmacology; D002478:Cells, Cultured; D004285:Dogs; D015870:Gene Expression; D006358:Hot Temperature; D008022:Ligaments / Q000166:cytology* / Q000378:metabolism; D008969:Molecular Sequence Data; D015676:Osteonectin / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D017670:Sodium Compounds
D018053:Arsenites; D015676:Osteonectin; D012333:RNA, Messenger; D017670:Sodium Compounds; D002104:Cadmium; C017947:sodium arsenite; D001383:Azetidinecarboxylic Acid; D001151:Arsenic