title: Cytokine stimulated endothelin release from endothelial cells.
abstract: Endothelin release from bovine endothelial cells of the aorta, pulmonary artery, and retinal microvessels was measured in response to various cytokines. Transforming growth factor beta (0.05-5 ng/ml) was found to be a potent stimulator (3-4 fold increase) of endothelin secretion in all three cell types. Tumour necrosis factor alpha (0.1-10 ng/ml) and interferon gamma (8-800 U/ml) had a small (1.5-2 fold increase) but significant effect on endothelin secretion from endothelial cells of large vessels but not the retinal microvessels. Interleukin-1 beta, Interleukin-6 and interleukin-8 at various doses did not affect endothelin secretion. These effects were observed at various time points from 6-24 hrs and indicate that of the cytokines tested, only transforming growth factor beta has a potent effect on endothelin release from endothelial cells of different organs.
authors.affiliation: Department of Endocrinology, Royal Postgraduate Medical School, London, United Kingdom.
authors.forename: S M
authors.initials: SM
authors.lastname: Kanse
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: H C
authors.initials: HC
authors.lastname: Lam
authors.forename: A
authors.initials: A
authors.lastname: Rees
authors.forename: J B
authors.initials: JB
authors.lastname: Warren
authors.forename: M
authors.initials: M
authors.lastname: Porta
authors.forename: P
authors.initials: P
authors.lastname: Molinatti
authors.forename: M
authors.initials: M
authors.lastname: Ghatei
authors.forename: S R
authors.initials: SR
authors.lastname: Bloom
mesh_terms: D001011:Aorta / Q000166:cytology; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D016232:Endothelins / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism*; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology; D007378:Interleukins / Q000494:pharmacology; D011651:Pulmonary Artery / Q000166:cytology; D012171:Retinal Vessels / Q000166:cytology; D012636:Secretory Rate / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D003470:Culture Media; D016232:Endothelins; D007378:Interleukins; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma