title: The gene encoding the Act-2 cytokine. Genomic structure, HTLV-I/Tax responsiveness of 5' upstream sequences, and chromosomal localization.
abstract: Act-2 is a cytokine that belongs to a superfamily of structurally related proteins. Act-2 expression is rapidly induced in T cells, B cells, and monocytes upon mitogenic stimulation. The Act-2 genomic locus is on chromosome 17q. The exons and exon/intron splice junctions have been sequenced, as have the sequences upstream of exon 1. A classical TATA box is located immediately upstream of the transcription initiation site. The upstream sequences possess promoter activity and can be functionally activated after treatment of Jurkat T cells with phythohemagglutinin plus phorbol myristrate acetate. In addition, Act-2 promoter chloramphenicol acetyltransferase constructs are expressed in human T cell lymphotropic virus type I (HTLV-I)-infected MT-2 cells and in Jurkat cells which can be induced to express the transactivator gene (tax) product of HTLV-I.
authors.affiliation: Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: M
authors.initials: M
authors.lastname: Napolitano
authors.forename: W S
authors.initials: WS
authors.lastname: Modi
authors.forename: S J
authors.initials: SJ
authors.lastname: Cevario
authors.forename: J R
authors.initials: JR
authors.lastname: Gnarra
authors.forename: H N
authors.initials: HN
authors.lastname: Seuanez
authors.forename: W J
authors.initials: WJ
authors.lastname: Leonard
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D054407:Chemokine CCL4; D019742:Chemokines, CC; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D016207:Cytokines / Q000235:genetics*; D004279:DNA, Viral; D005091:Exons; D015967:Gene Expression Regulation, Viral; D016355:Genes, pX; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D007438:Introns; D019402:Macrophage Inflammatory Proteins; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: C066507:CCL4 protein, human; D054407:Chemokine CCL4; D019742:Chemokines, CC; D016207:Cytokines; D004279:DNA, Viral; D019402:Macrophage Inflammatory Proteins; D011506:Proteins