Affinity purification of plasminogen by radial-flow affinity chromatography.
A method for the purification of plasminogen using immobilized L-lysine on a membrane, the whole system being constructed in a radial flow cartridge, is described. Human plasma was applied to the cartridge at 20 ml/min. The results showed that under the chromatographic conditions chosen, in a single pass, greater than 85% recovery of plasminogen was attained with a 110-fold increase in specific activity.
Planques
Y
Y
Institut Pasteur, Laboratoire de Microbiologie Moléculaire, Lille, France.
Pora
H
H
Menozzi
F D
FD
D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008239:Lysine / Q000378:metabolism; D010958:Plasminogen / Q000302:isolation & purification* / Q000378:metabolism
D010958:Plasminogen; D008239:Lysine