title: Phycoerythrins of marine unicellular cyanobacteria. III. Sequence of a class II phycoerythrin.
abstract: The genes for the alpha and beta subunits of a novel six bilin-bearing (class II) phycoerythrin were cloned from Synechococcus sp. WH8020 and sequenced. The cloned genes (mpeA and mpeB) were detected by homology with the genes for C-phycoerythrin from Pseudanabaena sp. PCC7409. The mpe locus occurs once in the genome and is arranged similarly to that of many other phycobiliproteins, with mpeA shortly 3' of mpeB. Sequence comparison suggests that this phycoerythrin (and perhaps all class II phycoerythrins) occupy a branch of the phycoerythrin family separate from five-chromophore per alpha beta (class I) phycoerythrins, C-phycoerythrin, and B-phycoerythrin. The position of the sixth chromophore of the class II phycoerythrin of WH8020 was determined by comparison of the amino acid sequence of the chromopeptides (Ong, L. J., and Glazer, A. N. (1991) J. Biol Chem. 266, 9515-9527) with the sequence deduced from the gene. This located the chromophore at residue 75 of the alpha subunit, very close to the alpha-83 chromophore in the primary structure and, presumably, in the three-dimensional structure.
authors.affiliation: Division of Biochemistry and Molecular Biology, University of California, Berkeley 94720.
authors.forename: S M
authors.initials: SM
authors.lastname: Wilbanks
authors.forename: R
authors.initials: R
authors.lastname: de Lorimier
authors.forename: A N
authors.initials: AN
authors.lastname: Glazer
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D000458:Cyanobacteria / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010799:Phycoerythrin / Q000235:genetics*; D011487:Protein Conformation; D015183:Restriction Mapping; D016415:Sequence Alignment
chemical_list: D010799:Phycoerythrin