Omeprazole treatment does not affect the metabolism of caffeine.
This study was performed to investigate the possible influence of repeated omeprazole dosing on the metabolism of caffeine, which has been shown to reflect the activity of one specific enzyme within the hepatic cytochrome P450 family, P450IA2. Ten healthy, nonsmoking young men participated in this placebo-controlled double-blind trial. Each subject was given omeprazole, 20 mg, every morning for 1 week and placebo every morning for 1 week in random order and separated by a 2-3 week washout period. On the sixth and seventh days of each period urine was collected twice daily, and urinary metabolites of caffeine were determined by high-performance liquid chromatography. The urinary metabolite ratio of three paraxanthine 7-demethylation products relative to a paraxanthine-hydroxylation product corresponds to caffeine clearance and, therefore, to P450IA2 activity. This calculated ratio was 4.8 (95% confidence interval, 3.9-5.6) in the placebo and 4.6 (95% confidence interval, 3.6-5.5) in the omeprazole period. These results show that the metabolism of caffeine was unaltered following omeprazole treatment, indicating that omeprazole treatment has no influence on cytochrome P450IA2 activity in the clinical situation.
Andersson
T
T
Research Laboratories, AB Hässle, Mölndal, Sweden.
Bergstrand
R
R
Cederberg
C
C
Eriksson
S
S
Lagerström
P O
PO
Skånberg
I
I
D000328:Adult; D001191:Arylamine N-Acetyltransferase / Q000378:metabolism; D002110:Caffeine / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D019388:Cytochrome P-450 CYP1A2; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004311:Double-Blind Method; D004790:Enzyme Induction; D006801:Humans; D008297:Male; D009853:Omeprazole / Q000494:pharmacology*; D010088:Oxidoreductases / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism
D002110:Caffeine; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D019388:Cytochrome P-450 CYP1A2; D014969:Xanthine Oxidase; D001191:Arylamine N-Acetyltransferase; D009853:Omeprazole