Role of the cytoskeleton in Ca2+ release from the intracellular Ca store of rat peritoneal mast cells.
In order to study the role of the cytoskeleton in histamine release from mast cells, the effects of cytochalasin D, cholchicine and vinblastine on Ca2+ release from the intracellular Ca store induced by compound 48/80 were investigated by means of a video-intensified microscopy system. When the quin 2-loaded mast cells were stimulated by 0.35 micrograms/ml of compound 48/80, a rapid increase in intracellular Ca2+ was observed. At concentrations higher than 10(-6) M, both colchicine and vinblastine pretreatments significantly inhibited the increase in intracellular Ca2+ concentrations caused by compound 48/80, although cytochalasin D had no effect. When permeabilized mast cells were exposed to potassium-antimonate solution, microtubules became attached to the endoplasmic reticulum, where many dots of Ca-antimonate were observed; in some areas, the microtubules interconnected the endoplasmic reticulum and granules in the mast cells. From the results of the present study, it was assumed that microtubules play some important role in the processes leading to Ca2+ release from the intracellular Ca store.
Tasaka
K
K
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Mio
M
M
Akagi
M
M
Fujisawa
K
K
Aoki
I
I
D000634:Aminoquinolines; D000818:Animals; D000965:Antimony; D002118:Calcium / Q000378:metabolism*; D002463:Cell Membrane Permeability; D003078:Colchicine / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology*; D005456:Fluorescent Dyes; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D008854:Microscopy, Electron; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D014747:Vinblastine / Q000494:pharmacology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D000634:Aminoquinolines; D005456:Fluorescent Dyes; C009002:potassium antimonate; D015638:Cytochalasin D; D003189:p-Methoxy-N-methylphenethylamine; D014747:Vinblastine; D000965:Antimony; C035739:Quin2; D003078:Colchicine; D002118:Calcium