A specific fluorogenic assay for N-acetylgalactosamine-4-sulphatase activity using immunoadsorption.
A method combining immune capture and enzyme detection by fluorochemistry has been developed for the diagnostic assay of N-acetylgalactosamine-4-sulphatase (4-sulphatase). The procedure uses a monoclonal antibody 4-S 4.1 to immunoadsorb 4-sulphatase specifically from complex protein samples containing other sulphatases, and 4-methylumbelliferyl sulphate to detect captured 4-sulphatase. The assay provides an accurate and simple method for the diagnosis of Maroteaux-Lamy syndrome (Mucopolysaccharidosis type VI).
Brooks
D A
DA
Department of Chemical Pathology, Adelaide Medical Centre for Women and Children, Australia.
Gibson
G J
GJ
McCourt
P A
PA
Hopwood
J J
JJ
D000911:Antibodies, Monoclonal; D002803:Chondro-4-Sulfatase / Q000032:analysis*; D006801:Humans; D006923:Hymecromone / Q000031:analogs & derivatives; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D009085:Mucopolysaccharidosis IV / Q000175:diagnosis / Q000276:immunology; D013050:Spectrometry, Fluorescence
D000911:Antibodies, Monoclonal; C019492:4-methylumbelliferyl sulfate; D006923:Hymecromone; D002803:Chondro-4-Sulfatase