Monoclonal anti-A and anti-A,B antibodies from a mouse immunized with A secretor saliva.
Using spleen cells from a mouse immunized with blood group A secretor saliva, we obtained 3 monoclonal anti-A antibodies (3H9, 5C6, 4D8) and 1 monoclonal anti-A,B antibody (2D10). In agglutination tests against red cells of A subgroups, 3H9 gave similar results to human anti-A serum, and 2D10 reacted with both A and B cells. 5C6 agglutinated especially A1 cells, while 4D8 showed stronger agglutination of A2 cells than the other antibodies. Using these antibodies, it is possible to distinguish the subgroups of A. The 4 antibodies had no reactivity with the red cells of 6 animal species and nonhuman primates except for group A anthropoid apes. All 4 antibodies were negative with Cad-positive, sheep and papain-treated O red cells that are agglutinated by the A-cell-reactive lectins. The activity of 3H9 was inhibited by A secretor saliva and that of 2D10 by both A and B secretor salivas. 4D8 showed a weak reaction with A secretor saliva. Antibody 5C6 was inhibited rather strongly even by A nonsecretor saliva. Thus, 5C6 is useful for application to test specimens in legal medicine, such as examination of blood or salivary stains for A antigens.
Miyazaki
S
S
Department of Legal Medicine, Gunma University School of Medicine, Maebashi, Japan.
Nakajima
T
T
Furukawa
K
K
D000017:ABO Blood-Group System / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000941:Antigens / Q000737:chemistry; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007114:Immunization; D051379:Mice; D008969:Molecular Sequence Data; D012463:Saliva / Q000276:immunology
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000941:Antigens; D002241:Carbohydrates