A primary encephalocele culture yields a pure population of human astrocytes.
In order to develop a reliable source of human astrocytes for in vitro studies, we established a primary explant culture of a human encephalocele. This culture yielded a population of cells which were karyotypically normal, morphologically resembled astrocytes, expressed glial fibrillary acid protein, and responded mitogenically to exogenous growth factors. We conclude that white matter derived from human encephaloceles can be used to generate pure populations of normal astrocytes.
Alterman
R L
RL
Leo M. Davidoff Department of Neurological Surgery, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY 10467.
Morrison
R S
RS
Goodrich
J T
JT
Papenhausen
P
P
Moskal
J R
JR
D001253:Astrocytes / Q000166:cytology* / Q000187:drug effects / Q000473:pathology; D015415:Biomarkers; D001921:Brain / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D004677:Encephalocele / Q000235:genetics / Q000473:pathology*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D007231:Infant, Newborn; D007621:Karyotyping; D008297:Male; D010751:Phosphopyruvate Hydratase / Q000032:analysis
D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D010751:Phosphopyruvate Hydratase