Macrophage chemotactic factor partially purified from granulomatous inflammation.
Pathophysiological roles of macrophage chemotactic factor (MCF) in granulomatous inflammation were investigated. MCF was extracted in 10 mM phosphate-buffered saline, pH 7.4, from experimentally produced epithelioid cell granulomas in the liver and skin of mice. MCF activity reached a peak in the lesions prior to the time when granulomatous inflammation became maximal. MCF was then purified from 10-week-old hepatic granulomas and 2-week-old skin lesions by gel filtration, ion exchange column chromatography, and HPLC gel filtration. MCF from either liver or skin had a molecular weight about 650 kDa. MCF from hepatic granulomas was coupled to Affi-Gel beads and transplanted subcutaneously into naive mice. In vivo macrophage chemotaxis was observed around the beads and the cells formed a sheet, but organization of macrophages into granulomas did not occur with the MCF-active fractions. Macrophage chemotaxis alone is insufficient to elicit granulomatous inflammation.
Sasaki
Y
Y
Department of Dermatology, University of California, San Francisco 94143-0536.
Iida
T
T
Sato
N
N
Fukuyama
K
K
Epstein
W L
WL
D000818:Animals; D002630:Chemotactic Factors / Q000302:isolation & purification*; D006099:Granuloma / Q000276:immunology* / Q000473:pathology; D008107:Liver Diseases / Q000276:immunology / Q000473:pathology; D008264:Macrophages / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000473:pathology; D012871:Skin Diseases / Q000276:immunology / Q000473:pathology
D002630:Chemotactic Factors