[Fusion of artificial lipid membranes induced by synthetic "fusion peptide" of arenaviruses].
The fusing capacity of lipid membranes of a synthetic 23-member peptide was studied. This hydrophobic peptide represents an analog of a predicted functional site ("fusion peptide") of the GP2 envelope protein of the Lassa virus (family Arenaviridae). Fusion of small monolayer liposomes was detected by the method of resonance energy transfer between the fluorescent derivatives of the lipid, NBD-PE (donor) and Rd-PE (acceptor). Using this peptide, the pH-dependent fusing activity was found in liposomes having different phospholipid composition. The rate and efficiency of liposome fusion increased with a decrease in pH and the lipid/peptide ratio as well as with a temperature increase. The increase in the ionic strength and Ca2+ concentration in the reaction mixture led to the inhibition of the peptide-induced fusion of liposomes. Neither the phospholipid charge, nor the transmembrane proton gradient of liposomes had any appreciable effect on the kinetics of the peptide-induced fusion. Neutralization of the medium in the course of the fusion reaction sharply decelerated, whereas repeated acidification activated this process. This finding suggests that peptide protonation plays a role in fusion reactions. It was suggested that acidification causes conformational changes in the peptide structure, thus activating the peptide-induced fusion of liposomes. The fusing capacity of the predicted Lassa virus fusion peptide is similar to that of viruses characterized by a pH-dependent step at the initial stages of the viral infection.
Glushakova
S E
SE
Omel'ianenko
V G
VG
Lukashevich
I S
IS
Bogdanov
A A
AA
Moshnikova
A B
AB
Kozhich
A T
AT
Torchilin
V P
VP
D000595:Amino Acid Sequence; D005454:Fluorescence Polarization; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007836:Lassa virus / Q000378:metabolism*; D008081:Liposomes; D008563:Membrane Lipids / Q000378:metabolism*; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D010455:Peptides / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D014759:Viral Envelope Proteins / Q000378:metabolism; D014760:Viral Fusion Proteins / Q000378:metabolism*
D008081:Liposomes; D008563:Membrane Lipids; D010455:Peptides; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins
Sliianie iskusstvennykh lipidnykh membran indutsiruemoe sinteticheskim "peptidom sliianiia" arenavirusov.