High-sensitivity laser-induced fluorescence detection for capillary electrophoresis.
Capillary electrophoresis provides very high efficiency separations of biological molecules. Laser-induced fluorescence produces very high sensitivity detection. The combination of the two techniques results in an analytical tool with unprecedented properties: separations with more than a million theoretical plates and detection limits of a few hundred analyte molecules. This paper considers the design of high-sensitivity laser-induced fluorescence detection for capillary zone electrophoresis separation of labeled amino acids and capillary gel electrophoresis separation of DNA sequencing fragments.
Zhang
J Z
JZ
Department of Chemistry, University of Alberta, Edmonton, Canada.
Chen
D Y
DY
Wu
S
S
Harke
H R
HR
Dovichi
N J
NJ
D000596:Amino Acids / Q000032:analysis; D004247:DNA / Q000032:analysis; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D007834:Lasers; D009696:Nucleic Acids / Q000032:analysis; D013050:Spectrometry, Fluorescence
D000596:Amino Acids; D009696:Nucleic Acids; D004247:DNA