[Incidence of hepatitis C virus antibodies in blood donors, high risk patient groups, liver diseases and health professionals. Multicenter study of the ABBOT ELISA method, comparison with the ORTHO test].
Serum samples from 1185 individuals (blood donors, health care workers, patients on haemodialysis or from other high risk groups or with non-A, non-B [NANB] hepatitis and other liver diseases) were examined for antibody to a recombinant antigen of hepatitis c virus (anti-HCV). A new ABBOTT HCV EIA system was used and a parallel study with ORTHO HCV ELISA was also done for 380 samples to compare the two anti-HCV tests. A confirmatory neutralizing ABBOTT ELISA probe was also performed in 45 cases. Anti-HCV seropositivity was found in 1.60% of accepted healthy blood donors, while among subjects excluded from donation for elevated aminotransferase the rate was 8.95%. In patients on haemodialysis 47.15% anti-HCV prevalence was found, in other high risk group subjects 32.5%. Patients with acute post-transfusion (PT) NANB hepatitis showed 40% prevalence, this rate in chronic PT-NANB was 77.8%. The two ELISA tests revealed 95% agreement in the parallel determinations. Serial dilution studies of anti-HCV positive sera showed that ABBOTT test was of superior sensitivity. The results of the confirmatory test suggest that reactive (positive) samples of low optical density near to the cut-off value require a confirmation with the neutralization test. In conclusion HCV infection in Hungary seems to be a common aetiologic factor in PT-NANB hepatitis and the screening of blood donors for anti-HCV may be useful. However, because of financial difficulties, cost/benefit calculations are recommended before the introduction of this preventive measure.
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Pécsi Orvostudományi Egyetem, I. Belklinika, Kántor Iidikó, ABBOTT Képviselet, Budapest.
D001782:Blood Donors; D004797:Enzyme-Linked Immunosorbent Assay; D006305:Health Status Indicators; D006280:Health Workforce; D006508:Hepatitis Antibodies / Q000276:immunology*; D006526:Hepatitis C / Q000276:immunology*; D006801:Humans; D006814:Hungary; D007159:Immunologic Tests; D008107:Liver Diseases / Q000276:immunology*; D012307:Risk Factors
D006508:Hepatitis Antibodies
Hepatitis C vírus antitest elöfordulása véradókban, nagy kockázatú betegscoportokban, májbetegekben és egészségügyi dolgozókban. Multicentrikus vizsgálat ABBOTT ELISA technikával, összehasonlítás ORTHO-teszttel.