Isolation and characterization of an established endothelial cell line from transgenic mouse hemangiomas.
A murine endothelial cell line was isolated from hemangiomas induced by expression of the polyoma early region gene in transgenic mice. After two cell sortings using acetylated low-density lipoprotein with a fluorescent label (Dil-Ac-LDL), a pure population of endothelial cells has been carried for more than 60 passages from the animal. The cells retain endothelial cell properties such as a characteristic cobblestone appearance at confluency, contact-inhibited growth, and active uptake of Ac-LDL. Expression analysis shows that the cells express both the polyoma transgene and the von Willebrand factor, an endothelial cell marker. Subcutaneous injection of the cultured endothelial cells into nontransgenic histocompatible mice or nude mice led to hemangioma formation, and endothelial cells were re-isolated by cell sorting from these secondary hemangiomas. This cell line represents a renewable source of murine endothelial cells derived from transgenic mice that can be studied both in vitro and by reintroduction into a host.
Dubois
N A
NA
Department of Biology, University of North Carolina, Chapel Hill 27599.
Kolpack
L C
LC
Wang
R
R
Azizkhan
R G
RG
Bautch
V L
VL
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D004727:Endothelium / Q000473:pathology; D005260:Female; D005800:Genes, Fungal; D006391:Hemangioma / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic; D009368:Neoplasm Transplantation; D016133:Polymerase Chain Reaction; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification
D000952:Antigens, Polyomavirus Transforming; D012334:RNA, Neoplasm