[Effects of gonadotropin and estrogen on endogenous growth factor synthesis in the porcine theca].
In order to investigate the effects of FSH, LH and estradiol (E2) on the synthesis of endogenous growth factor from the thecal tissue, porcine thecal tissues obtained from follicles (greater than 3mm) were cultured for 24 hours in serum-free media in the presence of various concentrations of FSH, LH and E2. After the incubation, thecal tissues and thecal conditioned media (CM) were collected. The growth-promoting activity of thecal CM and thecal cytosol of each conditioned group was assayed by measuring 3H-DNA synthesis in mouse BALB/c 3T3 cells. E2 increased the growth-promoting activity of thecal CM significantly in comparison with controls, whereas neither FSH nor LH showed any significant effects. However, the growth promoting activity in the cytosols was almost the same in all conditioned groups. These results indicate that E2 stimulates the synthesis and secretion of thecal growth factor without changing the original properties.
Yasumizu
T
T
Department of Obstetrics and Gynecology, Yamanashi Medical School.
Kato
J
J
D000818:Animals; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology* / Q000502:physiology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology* / Q000502:physiology; D006133:Growth Substances / Q000096:biosynthesis*; D007986:Luteinizing Hormone / Q000494:pharmacology* / Q000502:physiology; D013552:Swine; D013799:Theca Cells / Q000378:metabolism*
D006133:Growth Substances; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone