title: Protection and enhancement of ribulose 1,5 bisphosphate carboxylase activity by exogenous proteins.
abstract: When assayed in vitro, the activity of the photosynthetic enzyme ribulose 1,5 bisphosphate carboxylase oxygenase is both enhanced and protected from spontaneous decay by exogenous proteins such as hemoglobin, serum albumin, and aldolase. Other proteins and amino acids tested are either ineffective (lysozyme, ferritin, lysine, and cysteine) or afford only partial protection (catalase, glycine, and phenylalanine). Protective proteins do not bind to, or exchange disulfides with, ribulose 1.5 bisphosphate carboxylase/oxygenase. Since their effect can be mimicked by reductively treated detergents such as Triton X-100, it appears that proteins protect from decay by quenching the spontaneous oxidative degradation and inhibiting surface adsorption which could lead to enzyme unfolding. Release of adsorbed molecules from the container surface is likely to be the cause of carboxylase activity enhancement.
authors.affiliation: Departament de Bioquímica i Biologia Molecular, Facultats de Ciencies, Universitat de Valencia, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Peñarrubia
authors.forename: J
authors.initials: J
authors.lastname: Moreno
mesh_terms: D003902:Detergents / Q000494:pharmacology; D004220:Disulfides / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000494:pharmacology; D006454:Hemoglobins / Q000494:pharmacology; D007700:Kinetics; D017830:Octoxynol; D010047:Ovalbumin / Q000494:pharmacology; D010944:Plants / Q000201:enzymology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D011506:Proteins / Q000494:pharmacology*; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism*; D012709:Serum Albumin / Q000494:pharmacology; D013954:Thyroglobulin / Q000494:pharmacology; D014197:Trees
chemical_list: D003902:Detergents; D004220:Disulfides; D006454:Hemoglobins; D011506:Proteins; D012709:Serum Albumin; D011092:Polyethylene Glycols; D017830:Octoxynol; D010047:Ovalbumin; D013954:Thyroglobulin; D012273:Ribulose-Bisphosphate Carboxylase; D005634:Fructose-Bisphosphate Aldolase