Intracellular dCTP/ara-CTP ratio and the cytotoxic effect of ara-C.
In this study, the relationship between the dCTP/ara-CTP ratio and the cytotoxic effect of cytosine arabinoside (ara-C) was investigated. Intracellular levels of dNTPs and ara-CTP were analyzed by high-performance liquid chromatography. Although doubling time and intracellular dCTP levels were different in each of the cells, there was a consistent relation between the intracellular dCTP/ara-CTP ratio and the cytotoxic concentration of ara-C. These data suggest that the intracellular dCTP/ara-CTP ratio is one of the important factors for considering the cytotoxic action of ara-C.
Kawasaki
H
H
Department of Pediatrics, Mie University School of Medicine, Japan.
Kuwabara
H
H
Hori
H
H
Higashigawa
M
M
Ohkubo
T
T
Sakurai
M
M
D001085:Arabinofuranosylcytosine Triphosphate / Q000032:analysis* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry; D003561:Cytarabine / Q000378:metabolism / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000378:metabolism; D003845:Deoxycytosine Nucleotides / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism
D003470:Culture Media; D004273:DNA, Neoplasm; D003845:Deoxycytosine Nucleotides; D003561:Cytarabine; D001085:Arabinofuranosylcytosine Triphosphate; C024107:2'-deoxycytidine 5'-triphosphate