title: Identification and organization of carbon dioxide fixation genes in Xanthobacter flavus H4-14.
abstract: The genes encoding the large (cfxL) and small (cfxS) subunits of ribulose-1,5-bisphosphate carboxylase (RuBisC/O) from Xanthobacter flavus H4-14 were identified and characterized. The RuBisC/O genes are separated by 11 bp and cotranscribed in Escherichia coli from the lac promoter in the order cfxLS. Primer extension and R-loop experiments with RNA isolated from autotrophically grown X. flavus H4-14 showed that transcription of cfxL and cfxS initiated 22 bp upstream from cfxL and resulted in a mRNA of at least 2.3 kb. DNA sequence analysis identified the start of an open reading frame transcribed divergently from cfxL, and displaying significant similarities with genes belonging to the lysR family of transcriptional activators. Downstream from cfxS an additional open reading frame was identified with unknown function. Expression studies showed that the genes encoding fructosebisphosphatase (cfxF) and phosphoribulokinase (cfxP) are located downstream from cfxLS. The cfxF and cfxP genes are cotranscribed in the same direction as cfxLS in the order cfxFP.
authors.affiliation: Department of Microbiology, University of Groningen, The Netherlands.
authors.forename: W G
authors.initials: WG
authors.lastname: Meijer
authors.forename: A C
authors.initials: AC
authors.lastname: Arnberg
authors.forename: H G
authors.initials: HG
authors.lastname: Enequist
authors.forename: P
authors.initials: P
authors.lastname: Terpstra
authors.forename: M E
authors.initials: ME
authors.lastname: Lidstrom
authors.forename: L
authors.initials: L
authors.lastname: Dijkhuizen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002245:Carbon Dioxide / Q000378:metabolism*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D005798:Genes, Bacterial; D006088:Gram-Negative Aerobic Bacteria / Q000201:enzymology / Q000235:genetics* / Q000648:ultrastructure; D007700:Kinetics; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D002245:Carbon Dioxide; D012273:Ribulose-Bisphosphate Carboxylase