Different effects of DNA adducts induced by carcinogenic and noncarcinogenic azo dyes on in vitro DNA synthesis.
M13mp10 phage DNA modified with the carcinogen 3-methoxy-4-aminoazobenzene (3-MeO-AAB) or the noncarcinogen 2-methoxy-4-aminoazobenzene (2-MeO-AAB) was used as a template for E.coli DNA polymerase I. Analysis of the reaction products on DNA sequencing gels showed that with both types of compound the induced lesions blocked DNA synthesis, mainly at one base prior to guanine adducts, but that the inhibition by 3-MeO-AAB-adducts was substantially greater than that by 2-MeO-AAB-adducts. Thus different effects on DNA replication between 3-MeO-AAB- and 2-MeO-AAB-adducts might be a reflection of differences in their carcinogenic potency.
Kojima
M
M
Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Japan.
Degawa
M
M
Hashimoto
Y
Y
Tada
M
M
D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D002273:Carcinogens / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D004256:DNA Polymerase I / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects; D004277:DNA, Single-Stranded / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D008969:Molecular Sequence Data; D010128:p-Aminoazobenzene / Q000031:analogs & derivatives* / Q000494:pharmacology
D002273:Carcinogens; D004277:DNA, Single-Stranded; C053767:2-methoxy-4-aminoazobenzene; D010128:p-Aminoazobenzene; C044436:3-methoxy-4-aminoazobenzene; D004247:DNA; D004256:DNA Polymerase I