title: Detection and identification of loline and its analogues in horse urine.
abstract: Several kinds of loline-type alkaloids, norloline, loline, N-acetylnorloline, N-acetylloline, N-formylnorloline, N-formylloline and N-methylloline were detected in the urine of race-horses. Furthermore, a new compound of the alkaloids, N-senecioylnorloline, was also found and identified. These compounds were mainly identified by means of gas chromatography-mass spectrometry (GC-MS) and gas chromatography-fourier transform-infrared spectrometry (GC-FT-IR). A certain plant of Gramineae containing four kinds of loline-type alkaloids was found in a bale of hay used for the horse forage. The taxonomic feature of the plant was different from known plants containing loline-type alkaloids. The common fragmentation of loline-type alkaloids under electron ionization was briefly discussed.
authors.affiliation: Laboratory of Racing Chemistry, Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Takeda
authors.forename: E
authors.initials: E
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Kamei
authors.forename: H
authors.initials: H
authors.lastname: Nakata
mesh_terms: D000470:Alkaloids / Q000652:urine*; D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D006736:Horses / Q000652:urine*; D010944:Plants / Q000032:analysis; D013055:Spectrophotometry, Infrared
chemical_list: D000470:Alkaloids; C059478:loline