title: Development of an enzyme-linked immunosorbent assay of apolipoprotein E-AII complex in plasma.
abstract: Measurement of apolipoprotein (apo) E-AII complex in human plasma is important in determining the role of apoE in lipoprotein metabolism. In this paper, we demonstrate a new and simple method to determine apoE-All complex by using an enzyme-linked immunosorbent assay. Anti-apoE IgG (goat) was used as a capture antibody, and captured apoE-All complexes were detected by an anti-apoAll (rabbit) horseradish peroxidase-conjugated anti-rabbit IgG (goat) system. With this method, apoE-All complex was specifically determined without the interference of apoAll and was not affected by apoE monomer less than 250 mg/L. The content of the complex in reference serum, a normolipidemic serum pooled from five subjects with phenotype E3/E3, was arbitrarily defined as 100%. The coefficients of variation were 3.5%-6.3% within assay and 8.8%-11.6% between assays.
authors.affiliation: Central Clinical Laboratories, Shinshu University Hospital, Matsumoto, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tozuka
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshida
authors.forename: J
authors.initials: J
authors.lastname: Tanigami
authors.forename: M
authors.initials: M
authors.lastname: Miyachi
authors.forename: T
authors.initials: T
authors.lastname: Katsuyama
authors.forename: M
authors.initials: M
authors.lastname: Kanai
mesh_terms: D001057:Apolipoproteins E / Q000097:blood*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015203:Reproducibility of Results
chemical_list: D001057:Apolipoproteins E