title: Biosynthesis of marsupial milk oligosaccharides: characterization and developmental changes of two galactosyltransferases in lactating mammary glands of the tammar wallaby, Macropus eugenii.
abstract: Tammar wallaby (Macropus eugenii) mammary glands contain two galactosyltransferases of which the first, 4 beta GalT, is a UDP-galactose:N-acetylglucosaminyl beta 1----4-galactosyltransferase equivalent to the A protein of the lactose synthase of eutherian mammals. The second enzyme, 3 beta GalT, is a UDP-galactose:lactose beta 1----3-galactosyltransferase, not previously identified in mammary glands of any species, which catalyses the formation of Gal beta 1----3 Gal beta 1----4 Glc from lactose. The two enzyme activities, as well as the lactose synthase activity, have been characterised with respect to the effects of pH, apparent Km values, effects of bovine and tammar alpha-lactalbumins, heat sensitivity and identity of products. Studies on the substrate specificity and heat sensitivity of the 3 beta GalT activity suggest that this enzyme may catalyse the beta-galactosylation of Gal beta 1----3Gal beta 1----4Glc as well as of lactose. The activity of the 3 beta GalT, unlike that of the 4 beta GalT, changes dramatically during the course of lactation in parallel with similar changes in the carbohydrate content of tammar milk.
authors.affiliation: Department of Biochemistry, University of Sydney, N.S.W., Australia.
authors.forename: M
authors.initials: M
authors.lastname: Messer
authors.forename: K R
authors.initials: KR
authors.lastname: Nicholas
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002417:Cattle; D005260:Female; D005700:Galactosyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007768:Lactalbumin / Q000494:pharmacology; D007774:Lactation / Q000378:metabolism*; D007614:Macropodidae / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000201:enzymology / Q000502:physiology*; D008892:Milk / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000302:isolation & purification; D013379:Substrate Specificity
chemical_list: D009844:Oligosaccharides; D007768:Lactalbumin; D005700:Galactosyltransferases