[The induction of the monooxygenase system and the incorporation of the radioactive label from 2-14C-lysine into the liver microsome fraction of rats exposed to phenobarbital against a background of deficiencies in lysine, methionine, threonine and vitamins A, C and E].
The effect of diet on induction of monooxygenases and distribution of label from 2-14C-lysine in fractions of liver homogenate, muscle homogenate and blood of male rats treated with phenobarbital (80 mg/kg, three days) was studied. 2-14C-lysine was injected intraperitoneally 24 h before the first injection of phenobarbital. It was demonstrated that monoxygenase induction, increase of relative liver weight and incorporation of label from 2-14C-lysine into fractions of liver homogenate in phenobarbital-treated rats were more pronounced as compared with the similarly treated rats that were fed a balanced diet. The possibility of mobilization of deficient essential components to liver from other organs and tissues for maintenance of monoxygenase induction is discussed.
Nurmagambetov
T Zh
TZh
Amirov
B B
BB
Kuanysheva
T K
TK
Sharmanov
T Sh
TSh
D000596:Amino Acids / Q000172:deficiency*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001206:Ascorbic Acid Deficiency / Q000201:enzymology; D001361:Avitaminosis / Q000201:enzymology*; D002250:Carbon Radioisotopes / Q000493:pharmacokinetics*; D004790:Enzyme Induction / Q000187:drug effects / Q000502:physiology; D008239:Lysine / Q000172:deficiency / Q000378:metabolism*; D008297:Male; D008715:Methionine / Q000172:deficiency; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D010105:Oxygenases / Q000096:biosynthesis / Q000187:drug effects*; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013912:Threonine / Q000172:deficiency; D014802:Vitamin A Deficiency / Q000201:enzymology; D014811:Vitamin E Deficiency / Q000201:enzymology
D000596:Amino Acids; D002250:Carbon Radioisotopes; D013912:Threonine; D008715:Methionine; D010105:Oxygenases; D008239:Lysine; D010634:Phenobarbital
Induktsiia monooksigenaznoĭ sistemy i vkliuchenie radioaktivnoĭ metki iz 2-14C-lizina vo fraktsiiu mikrosom pecheni krys pri vozdeĭstvii fenobarbitala na fone defitsita lizina, metionina, treonina i vitaminov A, C i E.