title: Analysis of chloramphenicol residues in swine tissues and milk: comparative study using different screening and quantitative methods.
abstract: Both screening and quantitative methods for chloramphenicol residues in swine tissues and milk were compared, using samples from animals treated with chloramphenicol. For screening purposes a previously developed streptavidin-biotin enzyme-linked immunosorbent assay and a commercially available immunochemical card test were used. For quantitative purposes two previously developed high-performance liquid chromatographic procedures were applied using antibody-mediated clean-up and solid-phase extraction. Some improvements in both methods were also described. The results obtained with the screening tests and those obtained with the quantitative methods correspond well with each other. Using a combination of these methods, an effective control of residues of chloramphenicol can be performed in milk from the 1 microgram/kg level and in swine tissues from the 10 micrograms/kg level.
authors.affiliation: Faculty of Veterinary Medicine, Department of the Science of Food and Animal Origin, University of Utrecht, The Netherlands.
authors.forename: C
authors.initials: C
authors.lastname: van de Water
authors.forename: N
authors.initials: N
authors.lastname: Haagsma
mesh_terms: D000818:Animals; D001426:Bacterial Proteins; D001710:Biotin; D002701:Chloramphenicol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D007158:Immunologic Techniques; D008099:Liver / Q000737:chemistry; D008892:Milk / Q000737:chemistry*; D019809:Streptavidin; D013552:Swine / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D001426:Bacterial Proteins; D002701:Chloramphenicol; D001710:Biotin; D019809:Streptavidin