The case for a cause-effect linkage between environmental contamination and development in eggs of the common snapping turtle (Chelydra S.serpentina) from Ontario, Canada.
Concentrations of polychlorinated biphenyls (PCBs), dibenzo-p-dioxins, and dibenzofurans, organochlorine pesticides, and their metabolites were measured in eggs of the common snapping turtle (Chelydra s.serpentina) collected from four wetlands on the shorelines of Lakes Ontario, and Erie, and one control location in central Ontario, Canada. Snapping turtle eggs from these sites were also artificially incubated to determine hatching success, and incidence of deformities in embryo and hatchling turtles. The hypothesis that elevated incidences of egg death and/or deformities of hatchling turtles would occur in populations with high concentrations of organochlorine contaminants in eggs was tested. The results were elevated using epidemiological criteria. Unhatched eggs and deformities occurred at significantly higher rates in eggs from Lake Ontario wetlands. Two of three sites from Lake Ontario had substantially higher levels of PCBs, dioxins, and furans compared to eggs from Lake Erie and the control site. It could not be shown that contamination of eggs preceded the occurrence of poor development of eggs, although excellent hatching success and low numbers of deformities in eggs from the control site were considered representative of development in healthy eggs. The statistical association between contaminant levels in eggs and poor development of these eggs supported the hypothesis that eggs from sites with the greatest contamination had the highest rates of abnormalities. PCBs were the most strongly associated chemicals, although possible effects due to the presence of other chemicals in eggs was a confounding factor. The deformities and rates of unhatched eggs were similar to those occurring in other vertebrates collected from highly contaminated areas of the Great Lakes. There were several chemicals present in the eggs that can cause similar reproductive effects in other species; therefore a specific chemical effect was not identified. Results were coherent with known statistical and biological information. Theoretical and factual evidence of PCB contamination in wild-caught snapping turtles supported and hypothesis. However, lack of controlled studies of reproductive effects of polychlorinated hydrocarbons upon this species hindered the agreement of all factual and theoretical evidence with the hypothesis.
Bishop
C A
CA
Canadian Wildlife Service, Ontario Region, Canada.
Brooks
R J
RJ
Carey
J H
JH
Ng
P
P
Norstrom
R J
RJ
Lean
D R
DR
D000818:Animals; D015984:Causality; D004531:Eggs / Q000032:analysis*; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D005618:Fresh Water / Q000032:analysis; D007306:Insecticides / Q000032:analysis / Q000633:toxicity*; D009864:Ontario / Q000453:epidemiology; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000633:toxicity*; D012989:Soil Pollutants / Q000032:analysis*; D014426:Turtles / Q000002:abnormalities / Q000502:physiology*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
D007306:Insecticides; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls