Removal of psoralen monoadducts and crosslinks by human cell free extracts.
Human cell free extracts are capable of carrying out damage-induced DNA synthesis in response to DNA damage by UV, psoralen, and cisplatin. We show that this damage-induced DNA synthesis is associated with removal of psoralen adducts and therefore is 'repair synthesis' and not an aberrant DNA synthesis reaction potentiated by DNA deformed by adducts. By comparing the denaturable fraction of psoralen adducted DNA which becomes labeled in the repair reaction to that of terminally labeled DNA (without repair) we have found that all DNA synthesis induced by psoralen monoadducts is the consequence of removal of these adducts. By the same approach we have obtained preliminary evidence that this in vitro system is capable of removing psoralen crosslinks as well.
Reardon
J T
JT
Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599.
Spielmann
P
P
Huang
J C
JC
Sastry
S
S
Sancar
A
A
Hearst
J E
JE
D002474:Cell-Free System; D002945:Cisplatin / Q000494:pharmacology; D003432:Cross-Linking Reagents; D004247:DNA / Q000096:biosynthesis* / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage / Q000502:physiology*; D004260:DNA Repair / Q000502:physiology*; D004277:DNA, Single-Stranded / Q000032:analysis; D005363:Ficusin / Q000378:metabolism / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D004277:DNA, Single-Stranded; D004247:DNA; D005363:Ficusin; D002945:Cisplatin