Characterization of muscarinic receptor subtype of rat eccrine sweat gland by autoradiography.
The muscarinic cholinergic receptor of rat eccrine sweat gland was characterized using quantitative autoradiography and [3H]QNB as radioligand. The distribution of radioligand was maximal in the secretory coil. Autoradiographic competition binding studies were performed using selective antagonists to M1 (pirenzepine), M2 (AF-DX 116), and M3 (4-DAMP) and the classical nonselective antagonist atropine. pKi for pirenzepine, AF-DX 116, 4-DAMP, and atropine was 6.58, 5.47, 8.50, and 8.66 respectively indicating that the eccrine sweat gland muscarinic receptor was predominantly M3.
Torres
N E
NE
Department of Neurology, Mayo Foundation, Rochester, MN 55905.
Zollman
P J
PJ
Low
P A
PA
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001345:Autoradiography / Q000379:methods; D001667:Binding, Competitive; D004439:Eccrine Glands / Q000166:cytology / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D010276:Parasympatholytics / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D010890:Pirenzepine / Q000031:analogs & derivatives / Q000494:pharmacology; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D014316:Tritium
D010276:Parasympatholytics; D010880:Piperidines; D011976:Receptors, Muscarinic; D014316:Tritium; D010890:Pirenzepine; D011813:Quinuclidinyl Benzilate; D001285:Atropine; C042375:4-diphenylacetoxy-1,1-dimethylpiperidinium; C048593:otenzepad