Gas chromatographic analysis of malonaldehyde and 4-hydroxy-2-(E)-nonenal produced from arachidonic acid and linoleic acid in a lipid peroxidation model system.
Malonaldehyde (MA) and 4-hydroxynonenal (4-HN) formed upon oxidation with Fe2+/H2O2 from arachidonic acid and linoleic acid, and their ethyl esters were analyzed by gas chromatography (GC). The MA and 4-HN produced were reacted with N-methylhydrazine (NMH) to give 1-methylpyrazole and 5(1'-hydroxyhexyl)-1-methyl-2-pyrazoline, respectively. The derivatives were analyzed by GC on a fused silica capillary column using a nitrogen-phosphorus detector. With arachidonic acid, more MA and 4-HN were formed from the ester (88 nmol/mg and 23 nmol/mg, respectively) than from the free acid (25 nmol/mg and 9 nmol/mg, respectively). In contrast, with linoleic acid, more MA and 4-HN were produced from the free acid (53 nmol/mg and 13 nmol/mg, respectively) than from the ester (39 nm/mg and 8 nmol/mg, respectively).
Tamura
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Department of Environmental Toxicology, University of California, Davis 95616.
Shibamoto
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D000447:Aldehydes / Q000032:analysis*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000737:chemistry*; D002849:Chromatography, Gas; D006861:Hydrogen Peroxide; D007501:Iron; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000737:chemistry*; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000032:analysis*; D008954:Models, Biological; D015394:Molecular Structure
D000447:Aldehydes; D001095:Arachidonic Acids; D008041:Linoleic Acids; D016718:Arachidonic Acid; D008315:Malondialdehyde; D019787:Linoleic Acid; D006861:Hydrogen Peroxide; D007501:Iron; C027576:4-hydroxy-2-nonenal