Antibodies against Tetrahymena 14-nm filament-forming protein recognize the replication band in Euplotes.
Tetrahymena 14-nm filament-forming protein (49K protein) is a structural protein which is involved in activity of the pronuclei during conjugation (O. Numata, T. Sugai, and Y. Watanabe (1985) Nature (London) 314, 192-194). Using monoclonal and polyclonal antibodies, we here demonstrate the presence of a cross-reactive protein (CRP-49) within the macronuclear replication bands of Euplotes harpa and E. eurystomus which is recognized by anti-49K protein antibodies. Immunoblotting reveals that both monoclonal and polyclonal antibodies cross-react to a protein with an apparent molecular mass of 50 kDa in an E. harpa cell extract and to a protein of 49 kDa in a macronuclear extract of E. eurystomus. The antibodies used in this study have no effect upon in vitro DNA synthesis in the replication band of E. eurystomus.
Numata
O
O
Institute of Biological Sciences, University of Tsukuba, Japan.
Tomiyoshi
T
T
Kurasawa
Y
Y
Fu
Z X
ZX
Takahashi
M
M
Kosaka
T
T
Chiba
J
J
Watanabe
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D002467:Cell Nucleus / Q000737:chemistry; D016798:Ciliophora / Q000032:analysis* / Q000235:genetics / Q000648:ultrastructure; D003429:Cross Reactions; D004261:DNA Replication / Q000502:physiology*; D008840:Microfilament Proteins / Q000032:analysis*; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000032:analysis*; D013768:Tetrahymena / Q000032:analysis* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D008840:Microfilament Proteins; D015800:Protozoan Proteins