Activation of rainbow trout complement by C-reactive protein.
The activation of the complement system of rainbow trout by trout C-reactive protein (CRP) was investigated. Complement fixation tests were performed by using rabbit hemolysin-sensitized sheep erythrocytes and rainbow trout complement. Purified CRP increased the consumption of complement in the presence of Streptococcus pneumoniae C-polysaccharide (CPS), indicating the activation of the complement system. In contrast to this, acute phase serum activated the complement in the absence of CPS. Consumption of the complement by acute-phase serum was depressed when CRP was removed from acute-phase serum by CPS-sepharose 4B affinity chromatography. The acute-phase serum, as well as CRP plus CPS, suppressed in vitro growth of Vibrio anguillarum in the presence of complement, and enhanced the phagocytosis of the bacteria by glass-adherent peritoneal exudate cells. These results indicated that CRP has a role in host defense during acute-phase response through the activation of the complement system, enhancement of phagocytosis, and suppression of bacterial growth.
Nakanishi
Y
Y
Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Kodama
H
H
Murai
T
T
Mikami
T
T
Izawa
H
H
D000210:Acute-Phase Reaction / Q000276:immunology / Q000662:veterinary; D000818:Animals; D002097:C-Reactive Protein / Q000494:pharmacology*; D002448:Cell Adhesion; D002846:Chromatography, Affinity; D003167:Complement Activation / Q000187:drug effects*; D003168:Complement Fixation Tests; D005393:Fish Diseases / Q000276:immunology; D006461:Hemolysis; D010587:Phagocytosis; D014337:Trout / Q000276:immunology*; D014733:Vibrio / Q000276:immunology
D002097:C-Reactive Protein