T cell receptor V gene usage of islet beta cell-reactive T cells is not restricted in non-obese diabetic mice.
Five islet-reactive T cell clones were established from islet-infiltrating T cells of non-obese diabetic (NOD) mice. All clones expressed CD4, but not CD8, and responded to islet cells from various strains of mice in the context of I-ANOD. They could induce insulitis when transferred into disease-resistant I-E+ transgenic NOD mice. The T cell receptor (TCR) sequences utilized by the clones were determined. Their usage of TCR V and J segments was not restricted but was rather diverse. One of the clones utilized V beta 16. The expression of V beta 16 was significantly reduced in I-E+ transgenic NOD, suggesting the possibility that the islet-reactive T cell clone expressing V beta 16 may be deleted or inactivated by I-E molecules. This clone might be one of the candidates that triggers insulitis.
Nakano
N
N
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Kikutani
H
H
Nishimoto
H
H
Kishimoto
T
T
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D001402:B-Lymphocytes / Q000378:metabolism / Q000502:physiology*; D001483:Base Sequence; D015704:CD4 Antigens / Q000378:metabolism; D016827:CD8 Antigens; D002478:Cells, Cultured; D002872:Chromosome Deletion; D004247:DNA / Q000235:genetics; D003921:Diabetes Mellitus, Experimental / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D005260:Female; D015870:Gene Expression; D005808:Genes, Recessive / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000502:physiology; D013601:T-Lymphocytes / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure
D000945:Antigens, Differentiation, T-Lymphocyte; D015704:CD4 Antigens; D016827:CD8 Antigens; D011948:Receptors, Antigen, T-Cell; D004247:DNA