Steroid determinations in human ovarian follicular fluid using reversed phase liquid chromatography.
A method is presented, based on high performance liquid chromatography (HPLC) with u.v. absorbance detection, to simultaneously analyse all major unconjugated steroids in ovarian follicular fluids. The total analysis time is only 30 min. The use of a 3 mm i.d. column allows us to obtain detection limits for 3-oxo-4-ene steroids of 2 ng/ml. Calibration curves are linear in the 10-20,000 ng range per injection. Excellent agreement is obtained with the results using a previously published gaschromatography method.
Vanluchene
E
E
Department of Gynaecology, State University of Ghent, Belgium.
De Sutter
P
P
Dhont
M
M
Vandekerckhove
D
D
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry*; D006801:Humans; D013256:Steroids / Q000032:analysis*
D013256:Steroids