Interleukin-1 suppresses mesangial cell growth via inhibition of Ca2+ entry.
We have investigated the effect of interleukin-1 (IL-1) on the cell growth and Ca2+ homeostasis of rat mesangial cells in culture. DNA synthesis measured by [3H]thymidine uptake by mesangial cells was significantly inhibited by IL-1 (10 U/ml) and the calcium channel antagonist nicardipine (5 x 10(-6) M). 45Ca2+ uptake by mesangial cells was also significantly inhibited by IL-1 and nicardipine. The above observations support the premise that IL-1 suppresses the growth of mesangial cells via inhibition of extracellular Ca2+ entry to the cytosol.
Ikeda
M
M
Department of Medicine, Jichi Medical School, Tochigi, Japan.
Ikeda
U
U
Kano
S
S
D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects*; D004347:Drug Interactions; D005920:Glomerular Mesangium / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D009529:Nicardipine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013936:Thymidine / Q000378:metabolism
D007375:Interleukin-1; D000804:Angiotensin II; D009529:Nicardipine; D002118:Calcium; D013936:Thymidine