Ki-67 and bromodeoxyuridine labeling of human choroidal melanoma cells.
Understanding tumor growth patterns has implications for prognosis as well as for response and susceptibility to treatment. The antibody Ki-67 was used as a marker of cycling cells and bromodeoxyuridine (BrdUrd) was used as a marker of proliferating cells to characterize the cycling and proliferative rates of cells from human choroidal melanoma. The BrdUrd labeling indices varied from 0-1.1% and the Ki-67 labeling indices ranged from 0-3.0.3%. Linear regression modeling showed good correlation defined by the equation: Ki-67 index = 0.237 + 1.63 x BrdUrd labeling index with r = 0.919. Correlations between these indices and clinical and histologic parameters were not significant.
Bardenstein
D S
DS
Department of Ophthalmology, University of California, San Francisco.
Char
D H
DH
Kaleta-Michaels
S
S
Kroll
S M
SM
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D001973:Bromodeoxyuridine; D002830:Choroid Neoplasms / Q000175:diagnosis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D019394:Ki-67 Antigen; D008545:Melanoma / Q000175:diagnosis*; D009687:Nuclear Proteins; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; D001973:Bromodeoxyuridine