Effects of insulin-like growth factor I and growth hormone in cultured human granulosa cells.
Human granulosa cells, obtained either from natural or stimulated cycles, were cultured, and the response to IGF-I and GH was analyzed. It was found that IGF-I alone stimulated thymidine incorporation in both types of granulosa cells. Furthermore, IGF-I alone and in combination with FSH or LH enhanced estradiol and progesterone production. In a limited series of experiments, GH in combination with FSH was found to stimulate steroidogenesis in granulosa cells obtained from natural, but not from stimulated, cycles.
Bergh
C
C
Department of Obstetrics and Gynecology, University of Göteborg, Sweden.
Carlsson
B
B
Olsson
J H
JH
Billig
H
H
Hillensjö
T
T
D000328:Adult; D002478:Cells, Cultured; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D011374:Progesterone / Q000096:biosynthesis; D013256:Steroids / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism
D013256:Steroids; D011374:Progesterone; D004958:Estradiol; D007334:Insulin-Like Growth Factor I; D005640:Follicle Stimulating Hormone; D013006:Growth Hormone; D013936:Thymidine