In situ hybridization of Lilium whole mount synaptonemal complex chromosomal preparations.
Whole mount meiotic preparations of the synaptonemal complex complement of Lilium have been used for in situ hybridization experiments. A probe of the maize ribosomal DNA gene cluster has been successfully hybridized to the lily preparations. Three strong signals, corresponding to the three known lily nucleolus organizer regions, have been seen in most of the chromosome preparations. In situ hybridization experiments using meiotic preparations should be useful for identifying specific chromosomes, and for investigating the role of particular DNA molecules important to meiotic function.
Hasenkampf
C A
CA
Division of Life Sciences, University of Toronto, Ontario, Canada.
D006652:Histological Techniques; D008540:Meiosis; D009693:Nucleic Acid Hybridization; D015341:Nucleic Acid Probes; D009697:Nucleolus Organizer Region; D059828:Plant Cells; D013573:Synaptonemal Complex
D015341:Nucleic Acid Probes