title: [Study by NMR and circular dichroism of synthetic "zinc finger" peptide of viral Gag protein from HIV-2].
abstract: We report the synthesis in solid phase of an 18-amino acid peptide that contains the cysteine-rich region of the structural Gag protein of HIV-2. The characterization of this fragment and of its interaction with Zn2+ has been made by one- (1 D) and two-dimensional (2D) NMR techniques and by circular dichroism. Our results suggest that in aqueous solution the complexation produces a significant perturbation in the conformation of this peptide.
authors.affiliation: Laboratoire de Chimie de Coordination du C.N.R.S., Toulouse.
authors.forename: J P
authors.initials: JP
authors.lastname: Laussac
authors.forename: M T
authors.initials: MT
authors.lastname: Cung
authors.forename: M
authors.initials: M
authors.lastname: Erard
authors.forename: H
authors.initials: H
authors.lastname: Mazarguil
mesh_terms: D002942:Circular Dichroism; D015683:Gene Products, gag / Q000138:chemical synthesis*; D015498:HIV-2 / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D016335:Zinc Fingers
chemical_list: D015683:Gene Products, gag
vernacular_title: Etude par RMN et dichroïsme circulaire du peptide synthétique à "doigt de zinc" de la protéine virale Gag de VIH-2.