title: Recombinant DNA approaches to study the role of the regulatory light chains (RLC) using scallop myosin as a test system.
abstract: The ability to exchange reversibly the regulatory light chains (RLCs) from scallop myosin has provided us with a test system to probe the mechanisms of regulation mediated by the RLCs from vertebrate skeletal, vertebrate smooth and molluscan myosins. The cloning and expression of these RLCs, together with domain-swapping and site-directed mutagenesis approaches, has allowed us to explore further the mechanisms involved and identify the functional importance of specific regions of the RLC molecule; for example, the presence of a high affinity metal binding site in the N-terminal domain and its interaction with the intact C-terminal domains are required for regulation.
authors.affiliation: MRC Laboratory of Molecular Biology, Cambridge, UK.
authors.forename: J
authors.initials: J
authors.lastname: Kendrick-Jones
authors.forename: A C
authors.initials: AC
authors.lastname: Rasera da Silva
authors.forename: F C
authors.initials: FC
authors.lastname: Reinach
authors.forename: N
authors.initials: N
authors.lastname: Messer
authors.forename: T
authors.initials: T
authors.lastname: Rowe
authors.forename: P
authors.initials: P
authors.lastname: McLaughlin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000235:genetics*; D008969:Molecular Sequence Data; D008974:Mollusca; D009218:Myosins / Q000235:genetics*
chemical_list: D004274:DNA, Recombinant; D009218:Myosins