Transient expression of foreign DNA during embryonic and larval development of the medaka fish (Oryzias latipes).
Species of small fish are becoming useful tools for studies on vertebrate development. We have investigated the developing embryo of the Japanese medaka for its application as a transient expression system for the in vivo analysis of gene regulation and function. The temporal and spatial expression patterns of bacterial chloramphenicol acetyltransferase and galactosidase reporter genes injected in supercoiled plasmid form into the cytoplasm of one cell of the two-cell stage embryo was promoter-specific. The transient expression was found to be mosaic within the tissue and organs reflecting the unequal distribution of extrachromosomal foreign DNA and the intensive cell mixing movements that occur in fish embryogenesis. The expression data are consistent with data on DNA fate. Foreign DNA persisted during embryogenesis and was still detectable in some 3- and 9-month-old adult fish; it was found in high molecular weight form as well as in circular plasmid conformations. The DNA was replicated during early and late embryogenesis. Our data indicate that the developing medaka embryo is a powerful in vivo assay system for studies of gene regulation and function.
Winkler
C
C
Genzentrum, Max-Planck-Institut für Biochemie, Martinsried, FRG.
Vielkind
J R
JR
Schartl
M
M
D000818:Animals; D015139:Blotting, Southern; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D046508:Culture Techniques; D004247:DNA / Q000378:metabolism; D005786:Gene Expression Regulation; D008845:Microinjections; D009990:Oryzias / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D010957:Plasmids; D001616:beta-Galactosidase / Q000235:genetics
D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase; D001616:beta-Galactosidase