title: [Biofreezer BF 80--a universally employed apparatus in preservation at very low temperatures].
abstract: A computer-controlled freezing apparatus is described which uses liquid nitrogen (LN2) as coolant. The freezing equipment BF 80 consists of a big cryochamber (volume 24 l), a LN2 tank and a computer for the control of the freezing procedure. The biofreezer functions in the range from 40 to -180 degrees C. A complete cooling curve can be composed of up 15 linear segments with freezing rates between 0 and 45 K.min-1. In the computer up to 80 different freezing curves can be stored and activated by simple operations. The reliability of the biofreezer BF 80 was successfully tested for the cryopreservation of lymphocytes.
authors.affiliation: Forschungsabteilung Experimentelle Immunologie, Medizinischen Akademie Magdeburg.
authors.forename: U
authors.initials: U
authors.lastname: Küster
authors.forename: M
authors.initials: M
authors.lastname: Kunert
authors.forename: I
authors.initials: I
authors.lastname: Schönborn
authors.forename: P
authors.initials: P
authors.lastname: Weber
authors.forename: G
authors.initials: G
authors.lastname: Heyer
authors.forename: H W
authors.initials: HW
authors.lastname: Mansfeld
authors.forename: A
authors.initials: A
authors.lastname: Ittenson
authors.forename: S
authors.initials: S
authors.lastname: Ansorge
mesh_terms: D002470:Cell Survival; D003201:Computers; D015925:Cryopreservation / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology
vernacular_title: Biofreezer BF 80--eine universell einsetzbare Tieftemperaturkonservierungsapparatur.