Isolation and developmentally regulated expression of an Aspergillus nidulans phenol oxidase-encoding gene, ivoB.
Ivory (ivo) mutants of Aspergillus nidulans lack conidiophore pigmentation. We have cloned ivoB which codes for a conidiophore-specific phenol oxidase (AHTase) via the adjacent selectable ureD gene. Gene-library transformants of a ureD4 strain proved defective for the vector, but we recovered both ureD and ivoB from a lambda library of transformant DNA. The ivoB transcription unit was localized to a SalI-XbaI 3-kb fragment and its 5' end was located by hybridization with an oligodeoxyribonucleotide corresponding to the N-terminal polypeptide sequence of AHTase. Expression of the ivoB 1.4-kb mRNA corresponded temporally with AHTase in conidiating cultures, and the levels of both mRNA and AHTase in leaky brlA mutants implied transcriptional control by brlA. A second developmentally regulated locus of unknown function adjacent to ivoB was also transcriptionally dependent on brlA, but was expressed 4 h later.
Birse
C E
CE
Department of Genetics, Glasgow University, Scotland, U.K.
Clutterbuck
A J
AJ
D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D004156:Catechol Oxidase / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004271:DNA, Fungal; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010858:Pigmentation / Q000235:genetics; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D014157:Transcription Factors; D014158:Transcription, Genetic; D014170:Transformation, Genetic
C063497:BRLA protein, Emericella nidulans; D004271:DNA, Fungal; D005656:Fungal Proteins; D014157:Transcription Factors; D004156:Catechol Oxidase