Dermal and serological response against Pseudomonas aeruginosa in sheep bred for resistance and susceptibility to fleece-rot.
Genetically select lines of Merino sheep have been bred at Trangie (NSW Agriculture and Fisheries) for resistance (R) or susceptibility (S) to fleece-rot and flystrike. It is believed that fleece characters are primarily responsible for the R or S phenotype. When transferred to the wetter coastal environment of Sydney, R and S sheep with no more than 6 weeks wool cover, continued to show significant differences in the incidence and severity of fleece-rot dermatitis. To test the hypothesis that these sheep might also exhibit differences in their local skin reactions and immune responsiveness, 3 intradermal injections of killed Pseudomonas aeruginosa were administered at monthly intervals. After primary intradermal challenge, R sheep had a higher incidence of skin induration and a stronger inflammatory response (increased induration diameter) than S sheep. Compared to S sheep, R sheep also developed higher levels of circulating antibodies against whole cell antigen and both inner and outer membrane proteins of P. aeruginosa. These responses were maintained in R sheep with each consecutive challenge while S sheep showed a decline in their immune responsiveness. Differences in antibody response against outer membrane proteins were also detected when antigenically naive sheep from each genetic line were sensitised by epicutaneous challenge with P. aeruginosa under experimental wetting conditions. Intradermal challenge of these animals 6 months later with outer membrane proteins, revealed a late maximum (72 h) in the development of induration diameters for R sheep while S animals showed maximal induration diameters by 24 h. However, there was no significant difference in induration response between 24 h and 72 h within each group of sheep.(ABSTRACT TRUNCATED AT 250 WORDS)
Chin
J C
JC
Elizabeth Macarthur Agricultural Institute, Camden, New South Wales.
Watts
J E
JE
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001947:Breeding; D003872:Dermatitis / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D005260:Female; D020022:Genetic Predisposition to Disease; D007111:Immunity, Cellular; D007113:Immunity, Innate / Q000235:genetics; D007428:Intradermal Tests / Q000662:veterinary; D008297:Male; D011552:Pseudomonas Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D011550:Pseudomonas aeruginosa / Q000276:immunology; D012756:Sheep; D012757:Sheep Diseases / Q000235:genetics / Q000276:immunology*; D014935:Wool
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins