[The effect of low doses of ionizing radiation on the intermediate filaments and the Ca2+-activated proteolysis system in the rat brain].
The immunochemical methods were used to study the effect of low-level radiation (0.00645 C/kg and 0.0129 C/kg) on the content and polypeptide composition of glial intermediate filament proteins (GIFP) in different rat brain areas. Changes in glial fibrillar acidic protein (GFAP) concentration were more significant with the dose of 0.0129 C/kg than 0.00645 C/kg. It is suggested that soluble GIFP is more susceptible to the effect of Ca(2+)-dependent proteinases, calpains, than the filament one is, and degrades as early as the first few hours following irradiation. However, low radiation doses were ineffective with respect to calpains activity in the animal brain. The increased Ca2+ concentration enhances considerably GFAP degradation under the effect of calpains I and II. It is suggested that with low radiation doses the rearrangements of glial intermediate filaments may occur due to activation of calpains by releasing Ca ions.
Nedzvetskiĭ
V S
VS
Ushakova
G A
GA
Busygina
S G
SG
Berezin
V A
VA
Dvoretskiĭ
A I
AI
D000818:Animals; D001921:Brain / Q000378:metabolism / Q000528:radiation effects*; D002154:Calpain / Q000096:biosynthesis* / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D011829:Radiation Dosage; D051381:Rats; D011919:Rats, Inbred Strains
D005904:Glial Fibrillary Acidic Protein; D002154:Calpain
Vliianie malykh doz ioniziruiushcheĭ radiatsii na promezhutochnye filamenty i Ca2+-aktiviruemuiu sistemy proteoliza golovnogo mozga krysy.