title: Demonstration of concurrent dengue 1 and dengue 3 infection in six patients by the polymerase chain reaction.
abstract: A dual viremia resulting from naturally acquired dengue 1 and dengue 3 infections in six patients experiencing a dengue-like syndrome during the epidemic in New Caledonia in 1989 is reported. Serotype identification was first based on virus isolation in mosquito cells and immunofluorescence using type-specific monoclonal antibodies. The double infection was confirmed directly in blood samples by the polymerase chain reaction (PCR) on genomic RNA and hybridization of the amplified cDNA fragments with type-specific DNA probes.
authors.affiliation: Laboratoire des Arbovirus, Institut Pasteur de Nouvelle Calédonie, Nouméa, New Caledonia.
authors.forename: M
authors.initials: M
authors.lastname: Laille
authors.forename: V
authors.initials: V
authors.lastname: Deubel
authors.forename: F F
authors.initials: FF
authors.lastname: Sainte-Marie
mesh_terms: D001483:Base Sequence; D001769:Blood / Q000382:microbiology; D015342:DNA Probes; D004279:DNA, Viral / Q000737:chemistry; D003715:Dengue / Q000097:blood / Q000175:diagnosis*; D003716:Dengue Virus / Q000145:classification*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D015342:DNA Probes; D004279:DNA, Viral