The construction of a monoclonal diagnostic system for the field detection of Vibrio cholerae.
An enzyme-based double monoclonal field diagnostic system detecting both serotypes of Vibrio cholerae has been developed. The system uses nitrocellulose as a solid support, 1.25% skimmed dried milk as blocking reagent, water as washing reagent, and alkaline phosphatase cross-linked to antibody by means of glutaraldehyde as detecting reagent. The sensitivity of the system was 10(5) vibrios/ml. The biotin-avidin system gave sensitivity an order of magnitude weaker. There were no cross-reactions with the range of other bacteria tested.
al-Riyami
A
A
Department of Biochemistry, University of Glasgow, U.K.
Haynes
L G
LG
Campbell
A M
AM
D000469:Alkaline Phosphatase; D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001360:Avidin; D001710:Biotin; D003101:Collodion; D003429:Cross Reactions; D003432:Cross-Linking Reagents; D004797:Enzyme-Linked Immunosorbent Assay; D005976:Glutaral; D008892:Milk; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014734:Vibrio cholerae / Q000276:immunology* / Q000302:isolation & purification
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D011933:Reagent Kits, Diagnostic; D001360:Avidin; D001710:Biotin; D003101:Collodion; D000469:Alkaline Phosphatase; D005976:Glutaral