Preparation of lyophilized heat-treated cryoprecipitates from a small pool of plasma obtained by apheresis.
A method was developed for the batch-wise production of small-pool lyophilized heat-treated cryoprecipitates in a regional Blood Bank. Ten vials of final product were derived from twenty 600 g apheresis plasma donations. We found 384 IU of factor VIII per vial and a specific activity of 0.20 IU per mg total protein. Production recovery was 340 IU of factor VIII per kg plasma. The method was easy to perform and gave a product of good quality.
Allersma
D P
DP
Central Pharmacy of the Hospitals of The Hague, The Netherlands.
Roeffen
E
E
Van der Does
J A
JA
Briët
E
E
D001781:Blood Component Removal / Q000379:methods*; D005169:Factor VIII / Q000302:isolation & purification*; D005340:Fibrinogen / Q000302:isolation & purification*; D005612:Freeze Drying; D006358:Hot Temperature; D006801:Humans; D010956:Plasmapheresis; D011786:Quality Control
C026912:cryoprecipitate coagulum; D005169:Factor VIII; D005340:Fibrinogen