Isolation and characterization of nitrogen mustard-sensitive mutants of Chinese hamster ovary cells.
Three nitrogen mustard-sensitive lines of Chinese hamster ovary cells were isolated from mutagenized cultures using the procedure of Thompson et al. (1980). The lines, designated NM1, NM2 and NM3, were 2.1-, 17- and 6.8-fold more sensitive to nitrogen mustard, respectively, than their parent, wild-type, line as determined by the dose required to kill 90% of the cells, IC90. Patterns of cross-sensitivity to other DNA-damaging agents including ultraviolet light, cis-diamminedichloroplatinum, and other alkylating agents were determined for each line. Analysis of these results suggests that the phenotypes of the mutant lines are different from those lines reported previously.
Meyn
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Department of Experimental Radiotherapy, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Murray
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vanAnkeren
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Bernard
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GS
Mellard
D N
DN
Hobbs
M L
ML
D000585:Aminacrine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D002945:Cisplatin / Q000494:pharmacology; D006224:Cricetinae; D003429:Cross Reactions; D004247:DNA / Q000187:drug effects* / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005720:Gamma Rays / Q000009:adverse effects; D066298:In Vitro Techniques; D008466:Mechlorethamine / Q000494:pharmacology*; D008558:Melphalan / Q000494:pharmacology; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology
D008937:Mitomycins; D009153:Mutagens; D009588:Nitrogen Mustard Compounds; D008466:Mechlorethamine; D016685:Mitomycin; D000585:Aminacrine; D004247:DNA; C004294:acridine half-mustard; D002945:Cisplatin; D008558:Melphalan