title: The solution structure of a leucine-zipper motif peptide.
abstract: We report the complete structure determination of a 34 residue synthetic peptide with the amino acid sequence of the dimerization domain (leucine zipper) of GCN4. A high resolution structure in solution was obtained by 1H-NMR studies and distance geometry calculations followed by restrained energy minimization. A set of 20 final structures was obtained with an average root mean square deviation of 1.3 A for the backbone atoms (excluding the first and the last two residues). The structure contains an uninterrupted helix. A comparison with a structure previously determined for a larger peptide containing both the DNA-binding region (basic region) and the leucine-zipper motif shows the structural independence of the leucine-zipper domain from the contiguous DNA binding region.
authors.affiliation: Marion Merrell Dow Research Institute, Strasbourg, France.
authors.forename: V
authors.initials: V
authors.lastname: Saudek
authors.forename: A
authors.initials: A
authors.lastname: Pastore
authors.forename: M A
authors.initials: MA
authors.lastname: Morelli
authors.forename: R
authors.initials: R
authors.lastname: Frank
authors.forename: H
authors.initials: H
authors.lastname: Gausepohl
authors.forename: T
authors.initials: T
authors.lastname: Gibson
mesh_terms: D000595:Amino Acid Sequence; D004268:DNA-Binding Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D005656:Fungal Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D016350:Leucine Zippers; D009682:Magnetic Resonance Spectroscopy; D008433:Mathematics; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D011487:Protein Conformation; D011494:Protein Kinases; D029701:Saccharomyces cerevisiae Proteins; D012996:Solutions; D014157:Transcription Factors / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D010455:Peptides; D029701:Saccharomyces cerevisiae Proteins; D012996:Solutions; D014157:Transcription Factors; D011494:Protein Kinases