Actin-independent maturation of rabies virus in neuronal cultures.
This study outlines the effects of a modification of the actin-based cytoskeleton on the maturation of rabies virus in human neuroblastoma cell and primary rat cortical neuron cultures. In a Ca(2+)-depleted or an EGTA-containing medium, disruption of microfilaments did not affect intracellular viral nucleoprotein synthesis, as demonstrated by dual-immunofluorescence microscopy, and caused no change in the extracellular titre of rabies virus. Furthermore, the continuous presence of the anti-calmodulin drugs trifluoperazine (1 to 20 microM) and chlorpromazine (1 to 30 microM), or the L-type Ca2+ channel antagonist nifedepine (1 to 10 microM) or the Ca(2+)-specific ionophore A23187 (0.05 to 1.0 microM), did not modify the extracellular titre of rabies virus significantly over a 48 h period. The inference from these studies is that the maturation of rabies virus is independent of the integrity of the microfilament structures and calmodulin-dependent processes of neuronal cells.
Lockhart
B P
BP
Rabies Unit, Pasteur Institute, Paris, France.
Tsiang
H
H
D000199:Actins / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008854:Microscopy, Electron; D009447:Neuroblastoma; D009474:Neurons / Q000382:microbiology*; D011820:Rabies virus / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats; D014407:Tumor Cells, Cultured
D000199:Actins