title: Tyrosinase expression and melanogenesis in melanotic and amelanotic B16 mouse melanoma cells.
abstract: Tyrosinase activity, abundance, and mRNA transcription were examined in three sublines of the B16 mouse melanoma. Tyrosinase activity and melanin content were highest in the B16-F1 cells, slightly less in the B16-F10, and markedly lower in the B16-F10-DD cells. No differences in the level of tyrosinase mRNA or protein were found in the three different sublines. Thus, the differences in tyrosinase expression arise from the post-translational modification of the enzyme causing its activation or inhibition.
authors.affiliation: Department of Dermatology, University of Newcastle upon Tyne, UK.
authors.forename: S A
authors.initials: SA
authors.lastname: Burchill
authors.forename: D C
authors.initials: DC
authors.lastname: Bennett
authors.forename: A
authors.initials: A
authors.lastname: Holmes
authors.forename: A J
authors.initials: AJ
authors.lastname: Thody
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division; D015870:Gene Expression; D008543:Melanins / Q000032:analysis / Q000378:metabolism*; D008546:Melanoma, Experimental / Q000737:chemistry / Q000201:enzymology / Q000473:pathology*; D051379:Mice; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000378:metabolism*; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000201:enzymology / Q000473:pathology
chemical_list: D008543:Melanins; D012333:RNA, Messenger; D014442:Monophenol Monooxygenase