[The concept of lung and heart-lung preservation within the scope of multiple organ procurement].
Preservation of the lung is still one of the most challenging problems, because due to limited procurement time not all organs available can be used. The most common procurement technique is flush perfusion of the pulmonary artery system. Alternative methods in clinical use are either the autologous working heart-lung preparation or donor core-cooling (DCC). The own concept presented here, modified to the special demands of multi-organ-procurement, combines DCC and interstitial equilibration adapted to intracellular ion concentration. DCC is induced by extracorporeal circulation (ECC) using a transportable heart lung machine including a highly effective cooling system: cooling circuit based on two parallel heat exchangers with ice-water cooling produced by a high-pressure overflow of a low-temperature ice block (-40 degrees C). While cooling by ECC stepwise hemodilution is achieved by priming volume and incorporation of the cardioplegic solution (Bretschneider-HTK). The aim of equilibration is to lower the extracellular levels of sodium and calcium, and to increase the level of potassium. Additionally, the buffer capacity of donor blood is increased by the incorporated histidine-buffer system (alpha-stat). To avoid donor organ edema the time of ECC should be as short as possible. Using our system donor organ temperatures below 10 degrees C are reached within less than 30 min. In addition to ECC, lung surface cooling is achieved by external overflow with cold arterial blood (internal mammary artery). Besides lung preservation the main advantage of this concept is the profound precooling of all visceral organs before their individual flush perfusion.
Zerkowski
H R
HR
Abteilung für Thorax- und kardiovaskuläre Chirurgie, Universitätsklinikum Essen, Bundesrepublik Deutschland.
Doetsch
N
N
Hellinger
A
A
Reidemeister
J C
JC
D000818:Animals; D005947:Glucose; D016041:Heart-Lung Transplantation / Q000295:instrumentation*; D006801:Humans; D007036:Hypothermia, Induced / Q000295:instrumentation; D016040:Lung Transplantation / Q000295:instrumentation*; D008353:Mannitol; D009926:Organ Preservation / Q000295:instrumentation*; D011189:Potassium Chloride; D011343:Procaine; D012756:Sheep; D013552:Swine; D013556:Swine, Miniature; D014022:Tissue Survival / Q000502:physiology; D009927:Tissue and Organ Procurement / Q000379:methods
C039984:Bretschneider cardioplegic solution; D008353:Mannitol; D011343:Procaine; D011189:Potassium Chloride; D005947:Glucose
Konzept zur Lungen- und Herz-Lungen-Präservation im Rahmen der Multiorganentnahme.