title: Generation of novel sequence tagged sites (STSs) from discrete chromosomal regions using Alu-PCR.
abstract: Human DNA segments from discrete chromosomal regions were generated by utilizing Alu-element-based polymerase chain reaction (Alu-PCR) of an irradiation-fusion hybrid containing approximately 10 to 15 Mb of human DNA. Following cloning into a plasmid vector, a subset of the clones was used to generate sequence tagged sites (STSs) de novo. By means of a panel of hybrids containing portions of the human X chromosome, the STSs were shown to localize to two chromosomal regions, Xq24-Xq26 and Xcen-Xq13, reflecting the presence in the irradiation-fusion hybrid of two human chromosome fragments. These results demonstrate that high densities of STSs can be rapidly and efficiently generated from defined regions of the human genome using Alu-PCR.
authors.affiliation: Paediatric Research Unit, United Medical School, Guy's Hospital, London, United Kingdom.
authors.forename: C G
authors.initials: CG
authors.lastname: Cole
authors.forename: P N
authors.initials: PN
authors.lastname: Goodfellow
authors.forename: M
authors.initials: M
authors.lastname: Bobrow
authors.forename: D R
authors.initials: DR
authors.lastname: Bentley
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D005819:Genetic Markers; D015894:Genome, Human; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D016324:Sequence Tagged Sites; D014960:X Chromosome
chemical_list: D005819:Genetic Markers