Different expression time of the 105-kDa protein and 90-kDa heat-shock protein in rat testis.
To understand the physiological functions of the 105-kDa protein which is testis-specific and HSP90 (90-kDa heat-shock protein) related protein, the appearance of it in the testis has been followed during the development of rat. On immunoblotting analysis, the 105-kDa protein did not appear until after the age of five weeks, while HSP90 could be detected at three weeks. In the spermatozoa, the 105-kDa protein was much abundant but not in the LC-540 cells (a cell line from Leydig cell tumor in rat testis) cytosol. This finding has attracted much attention to the relationship between this protein and sperm functions.
Itoh
H
H
Department of Biochemistry, Akita University School of Medicine, Japan.
Tashima
Y
Y
D000818:Animals; D015153:Blotting, Western; D002470:Cell Survival; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000502:physiology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000254:growth & development / Q000378:metabolism*
D006360:Heat-Shock Proteins