Superoxide dismutases of virulent and avirulent strains of Brucella abortus.
Extracts of Brucella abortus strains 2308,RB51,45/20 and ST 19 had no significant differences in superoxide dismutase (SOD) activity as measured by the epinephrine assay. These B. abortus strains represent smooth, intermediate and rough colony forms. SOD activity was inhibited 60 to 75% by 2 mM KCN and suggests the presence of Cu/Zn SOD. The SOD activities were similar when the strains were grown in trypticase soy broth containing either 0.5% glucose or erythritol. There were two distinct SOD activity bands in native polyacrylamide gel electrophoresis with identical mobilities for each of the strains. When the native gel was stained for SOD activities in the presence of 2 mM KCN, the SOD band that co-migrated with the bovine erythrocyte Cu/Zn SOD activity disappeared. The band of SOD activity that migrated similar to E. coli iron SOD activity was unaffected by KCN. There were no significant differences in either the total SOD or Cu/Zn SOD activities among the strains. As the Brucella strains represent ranges of virulence, it is difficult to associate any primary role for SOD as a virulence factor.
Sriranganathan
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N
Department of Pathobiology, Va-Md Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Boyle
S M
SM
Schurig
G
G
Misra
H
H
D000704:Analysis of Variance; D002003:Brucella abortus / Q000201:enzymology* / Q000254:growth & development / Q000472:pathogenicity; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D004896:Erythritol / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D011190:Potassium Cyanide / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000032:analysis* / Q000037:antagonists & inhibitors; D014774:Virulence
D013482:Superoxide Dismutase; D005947:Glucose; D011190:Potassium Cyanide; D004896:Erythritol