title: Endothelial albumin permeability is shear dependent, time dependent, and reversible.
abstract: Altered permeability of vascular endothelium to macromolecules may play a role in vascular disease as well as vascular homeostasis. Because the shear stress of flowing blood on the vascular wall is known to influence many endothelial cell properties, an in vitro system to measure transendothelial permeability (Pe) to fluorescein isothiocyanate conjugated bovine serum albumin under defined physiological levels of steady laminar shear stress was developed. Bovine aortic endothelial cells grown on polycarbonate filters pretreated with gelatin and fibronectin constituted the model system. Onset of 1 dyn/cm2 shear stress resulted in a Pe rise from 5.1 +/- 1.3 x 10(-6) cm/s to 21.9 +/- 4.6 X 10(-6) cm/s at 60 min (n = 6); while 10 dyn/cm2 shear stress increased Pe from 4.8 +/- 1.5 X 10(-6) cm/s to 50.2 +/- 6.8 X 10(-6) cm/s at 30 min and 49.6 +/- 8.9 X 10(-6) cm/s at 60 (n = 9). Pe returned to preshear values within 120 and 60 min after removal of 1 and 10 dyn/cm2 shear stress, respectively. The data show that endothelial cell Pe in vitro is acutely sensitive to shear stress.
authors.affiliation: Department of Biology, Pennsylvania State University, University Park 16802.
authors.forename: H
authors.initials: H
authors.lastname: Jo
authors.forename: R O
authors.initials: RO
authors.lastname: Dull
authors.forename: T M
authors.initials: TM
authors.lastname: Hollis
authors.forename: J M
authors.initials: JM
authors.lastname: Tarbell
mesh_terms: D000418:Albumins / Q000493:pharmacokinetics*; D000818:Animals; D002417:Cattle; D002463:Cell Membrane Permeability / Q000502:physiology*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D013861:Thiocyanates
chemical_list: D000418:Albumins; D005452:Fluoresceins; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate