Use of a lacZ fusion to study transcriptional regulation of the Rhodobacter capsulatus hemA gene.
An EcoRI fragment containing the Rhodobacter capsulatus hemA promoter has been cloned into a lacZ translational fusion vector. The resulting plasmid produced a hemA-lacZ fusion protein with a molecular mass of 147,000. Expression of the hemA-lacZ fusion, as measured by production of beta-galactosidase, was regulated 2- to 3-fold by oxygen tension. The unexpectedly small change in beta-galactosidase levels suggests that transcriptional regulation of the hemA gene is not the major factor in oxygen-mediated control of porphyrin synthesis.
Wright
M S
MS
Department of Microbiology, Louisiana State University, Baton Rouge 70803.
Eckert
J J
JJ
Biel
S W
SW
Biel
A J
AJ
D000624:5-Aminolevulinate Synthetase / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010100:Oxygen / Q000494:pharmacology; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016354:Rhodobacter capsulatus / Q000235:genetics*; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D004269:DNA, Bacterial; D011993:Recombinant Fusion Proteins; D000624:5-Aminolevulinate Synthetase; D001616:beta-Galactosidase; D010100:Oxygen