Distinct cellular expression of pertussis toxin-sensitive GTP-binding proteins in rat cerebellum.
Immunohistochemical localization of pertussis toxin-sensitive GTP-binding proteins in rat cerebellum was investigated using antibodies raised against purified G alpha o and synthetic peptides corresponding to specific sequences of G alpha i1, G alpha i2 and G alpha o was detected mostly in the molecular layer but not in the cell body of the Purkinje cells. G alpha i1 and G alpha i2 were found predominantly in the molecular layer and in addition in the cell body of the Purkinje cells. G alpha i3 was not detected in rat cerebellum. Immunoblot analysis demonstrated that in the cerebellum membrane G alpha 0 was most abundant and surprisingly the amount of G alpha i2 was much higher than that of G alpha i1. Thus, each pertussis toxin substrate was found to be expressed differently in rat cerebellum. The different patterns of expression imply that each subtype of GTP-binding proteins may have a specific function in modulating the neuronal activity in rat cerebellum.
Nishida
A
A
Department of Psychiatry and Neuroscience, Kure National Hospital, Japan.
Kaiya
H
H
Kanaho
Y
Y
Nozawa
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000187:drug effects; D008969:Molecular Sequence Data; D037342:Pertussis Toxin; D011689:Purkinje Cells / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015398:Signal Transduction; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins