Effects of hachimijiogan, tokishakuyakusan, keishibukuryogan, ninjinto and unkeito on estrogen and progesterone secretion in preovulatory follicles incubated in vitro.
Ovarian follicles, removed from 10-week old rats at 1630 hours diestrus-2, 1100 and 2300 hours proestrus, were incubated for 120 minutes with various doses of Hachimijiogan (HJ), Tokishakuyakusan (TS), Keishibukuryogan (KB), Ninjinto (NT) and Unkeito (UT). The estradiol-17 beta (E2) and progesterone concentrations in the incubation medium were measured. The concentrations of E2 were significantly decreased with TS and KB by growing follicles and with HJ, TS and KB by preovulatory follicles before a LH surge. In contrast, the levels of progesterone were significantly increased with HJ, TS, KB and UT by preovulatory follicles before a LH surge. These results suggest that HJ, TS, KB or UT stimulates preovulatory follicles before a LH surge to secrete progesterone, but TS or KB suppresses E2 secretion by growing preovulatory follicles before a LH surge.
Usuki
S
S
Department of Obstetrics and Gynecology, University of Tsukuba, Ibaraki, Japan.
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D005498:Follicular Phase / Q000187:drug effects*; D011374:Progesterone / Q000378:metabolism*; D051381:Rats
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004365:Drugs, Chinese Herbal; D004967:Estrogens; C054591:hachimijiogan; C413220:toki-shakuyaku-san; C058415:unkei-to; D011374:Progesterone