title: Rel-associated pp40: an inhibitor of the rel family of transcription factors.
abstract: The Rel-associated protein pp40 is functionally related to I kappa B, an inhibitor of the transcription factor NF-kappa B. Purified pp40 inhibits the DNA binding activity of the NF-kappa B protein complex (p50:p65 heterodimers), p50:c-Rel heteromers, and c-Rel homodimers. The sequence of the complementary DNA encoding pp40 revealed similarity to the gene encoding MAD-3, a protein with mammalian I kappa B-like activity. Protein sequencing of I kappa B purified from rabbit lung confirmed that MAD-3 encodes a protein similar to I kappa B. The sequence similarity between MAD-3 and pp40 includes a casein kinase II and consensus tyrosine phosphorylation site, as well as five repeats of a sequence found in the human erythrocyte protein ankyrin. These results suggest that rel-related transcription factors, which are capable of cytosolic to nuclear translocation, may be held in the cytosol by interaction with related cytoplasmic anchor molecules.
authors.affiliation: Department of Microbiology, University of Texas, Austin 78712.
authors.forename: N
authors.initials: N
authors.lastname: Davis
authors.forename: S
authors.initials: S
authors.lastname: Ghosh
authors.forename: D L
authors.initials: DL
authors.lastname: Simmons
authors.forename: P
authors.initials: P
authors.lastname: Tempst
authors.forename: H C
authors.initials: HC
authors.lastname: Liou
authors.forename: D
authors.initials: D
authors.lastname: Baltimore
authors.forename: H R
authors.initials: HR
authors.lastname: Bose
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002642:Chick Embryo; D003001:Cloning, Molecular; D015342:DNA Probes; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D015345:Oligonucleotide Probes; D020572:Oncogene Proteins v-rel; D016366:Open Reading Frames; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D012333:RNA, Messenger / Q000235:genetics; D015682:Retroviridae Proteins, Oncogenic / Q000037:antagonists & inhibitors*; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000037:antagonists & inhibitors*
chemical_list: D015342:DNA Probes; D016328:NF-kappa B; D015345:Oligonucleotide Probes; D020572:Oncogene Proteins v-rel; D010750:Phosphoproteins; D012333:RNA, Messenger; D015682:Retroviridae Proteins, Oncogenic; D014157:Transcription Factors; C070928:Rel-associated protein pp40; D011505:Protein-Tyrosine Kinases