title: Bioassay for siderophore utilization by Candida albicans.
abstract: A convenient plate assay which is sensitive to medium pH has been developed to evaluate potential siderophores of Candida albicans. Adding a siderophore to a filter paper disk on chemically defined Lee's agar (final pH 7.2) seeded with the test strain reversed the growth inhibitory effects of the supplemented (25-100 micrograms/ml) iron chelator ethylenediaminedi(o-hydroxyphenylacetic acid), to provide a zone of growth stimulation. This bioassay has been used to demonstrate the structure-activity relationships of ferrichrome and several water-soluble hydroxamate peptide building blocks of this natural siderophore. Of all compounds so evaluated, ferrichrome exhibited the best activity.
authors.affiliation: Department of Chemistry and Biochemistry, University of Notre Dame, Indiana 46556.
authors.forename: A A
authors.initials: AA
authors.lastname: Minnick
authors.forename: L E
authors.initials: LE
authors.lastname: Eizember
authors.forename: J A
authors.initials: JA
authors.lastname: McKee
authors.forename: E K
authors.initials: EK
authors.lastname: Dolence
authors.forename: M J
authors.initials: MJ
authors.lastname: Miller
mesh_terms: D002176:Candida albicans / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D004492:Edetic Acid / Q000494:pharmacology / Q000031:analogs & derivatives; D005291:Ferrichrome / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism*; D008670:Metals / Q000378:metabolism; D008722:Methods; D010455:Peptides / Q000378:metabolism
chemical_list: D008670:Metals; D010455:Peptides; D005291:Ferrichrome; C026058:EDDA; D004492:Edetic Acid; D007501:Iron