Hormone-associated variation of the glycan microheterogeneity pattern of human sex steroid-binding protein (hSBP).
hSBP is a steroid-binding protein (human) whose serum concentration is increased by estrogens and decreased by androgens. This regulation is independent of a direct effect on the hSBP gene transcription. The purpose of this work was to study the glycan microheterogeneous composition of the mature protein under physiological estrogen stimulation, by means of crossed affinoimmunoelectrophoresis using concanavalin-A. In men hSBP always divided into 2 fractions, both retarded. In women hSBP showed two other components, still more retarded. An explanation for these differences is given and the role of the glycan moiety of hSBP is discussed.
Tardivel-Lacombe
J
J
Laboratoire de Biochimie Endocrinienne, Faculté de Médecine, Paris, France.
Degrelle
H
H
D003208:Concanavalin A; D005260:Female; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007700:Kinetics; D046911:Macromolecular Substances; D008297:Male; D011134:Polysaccharides / Q000032:analysis; D011247:Pregnancy; D012016:Reference Values; D012738:Sex Hormone-Binding Globulin / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D046911:Macromolecular Substances; D011134:Polysaccharides; D012738:Sex Hormone-Binding Globulin; D003208:Concanavalin A