Analysis of immunoglobulin heavy chain delta transcription termination in the production of delta S or delta M mRNA.
mRNA encoding secreted immunoglobulin is synthesized either by termination of transcription 3' to secreted terminus sequences and 5' to the membrane terminus sequences or by cleavage of a pre-mRNA transcript containing both secreted and membrane sequences at the appropriate polyadenylation site 5' to the membrane sequences. In vitro "run-on" transcription analysis was used to examine the delta transcription termination patterns in resting membrane IgD expressing B lymphocytes, in KWD2, an IgD-secreting hybridoma, and in TEPC 1017, an IgD-secreting plasmacytoma. In resting B cells, transcription terminated in a region 4 to 7 kilobases 3' to the delta M exons. Transcription in the secreting cells continued through the delta M exons, but terminated at more upstream sites. Additionally, an increased loading of polymerases in the region of the delta S exon and its 5' flanking sequence was detected in the secreting cells and was particularly pronounced in TEPC 1017. It is hypothesized that this peak correlates with high delta S mRNA production.
Weiss
E A
EA
Graduate Program in Immunology, University of Texas, Southwestern Medical Center, Dallas 75235.
Tucker
P W
PW
Finkelman
F D
FD
Yuan
D
D
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D015152:Blotting, Northern; D015139:Blotting, Southern; D002460:Cell Line; D005260:Female; D007072:Immunoglobulin D / Q000235:genetics* / Q000378:metabolism; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007138:Immunoglobulin delta-Chains / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D015335:Molecular Probes; D011061:Poly A / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis*; D015183:Restriction Mapping; D013154:Spleen / Q000166:cytology; D013728:Terminator Regions, Genetic
D000546:Amanitins; D007072:Immunoglobulin D; D007143:Immunoglobulin Heavy Chains; D007138:Immunoglobulin delta-Chains; D015335:Molecular Probes; D012333:RNA, Messenger; D011061:Poly A