title: Thrombin decreases glycosaminoglycans content of endothelial cells in culture.
abstract: The effect of thrombin on the metabolism of glycosaminoglycans (GAG) in cultured bovine aortic endothelial cells was investigated. Incubation of confluent endothelial cell cultures with thrombin at 1.0 NIH U/ml for 8 h and above caused a significant decrease in the accumulation of [35S]sulfate-labeled GAG (35S-GAG) in the cell layer. After a 24 h incubation, thrombin at 0.5 U/ml and above decreased the accumulation of 35S-GAG in both cell layer and medium. The percentage of 35S-GAG released into the medium during the last 3 h of 24 h incubation was significantly increased by thrombin. In thrombin-treated cell layer, both heparan sulfate and the other GAG was significantly decreased at the same degree. The incorporation of both [3H]thymidine and [14C]leucine was significantly increased by thrombin. Gabexate mesilate, a serine protease inhibitor, suppressed the thrombin inhibition. From these results, it was suggested that thrombin decreased 35S-GAG in the endothelial cell layer without a nonspecific damage of the cells through mainly an inhibition of GAG production and additionally a stimulation of GAG release.
authors.affiliation: Department of Environmental Science, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kaji
authors.forename: T
authors.initials: T
authors.lastname: Akai
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
authors.forename: N
authors.initials: N
authors.lastname: Sakuragawa
mesh_terms: D000818:Animals; D001011:Aorta; D002417:Cattle; D002478:Cells, Cultured / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D016670:Gabexate; D006025:Glycosaminoglycans / Q000378:metabolism*; D006146:Guanidines / Q000494:pharmacology*; D014176:Protein Biosynthesis; D013917:Thrombin / Q000494:pharmacology*
chemical_list: D006025:Glycosaminoglycans; D006146:Guanidines; D016670:Gabexate; D004247:DNA; D013917:Thrombin