Analysis of pH-induced population oscillations of Saccharomyces cerevisiae and Escherichia coli using photon correlation spectroscopy.
Following a recent successful application of p.c.s. to liquid chromatography in the biotechnology industry, its usefulness as a contamination monitor in the fermentation industry was assessed. It was found that: (i) the intensity bias of the technique limits its uses to the detection of contaminants when they are larger than the host; (ii) the inherent heterogeneity of microbial cultures prevents the use of multiangle studies, and (iii) the large size of bacteria make the use of p.c.s. in flowing, on-line systems impractical.
Jepras
R I
RI
Division of Biotechnology, P.H.L.S. Centre for Applied Microbiology and Research, Porton Down, Wilts, U.K.
Rarity
J
J
Perkins
E A
EA
Clarke
D J
DJ
Carr
R J
RJ
Atkinson
T
T
D015502:Absorptiometry, Photon / Q000379:methods; D004926:Escherichia coli / Q000254:growth & development*; D005285:Fermentation; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000254:growth & development*; D013997:Time Factors