title: A geranylgeranyltransferase for rhoA p21 distinct from the farnesyltransferase for ras p21S.
abstract: We have clarified that rhoA p21 purified from bovine aortic smooth muscle is geranylgeranylated at the cysteine residue in the C-terminal CAAX motif (A is an aliphatic amino acid and X is any amino acid). In this paper, a geranylgeranyltransferase for rhoA p21 (rhoA p21 GGT) was partially purified from bovine brain cytosol. This enzyme transferred a geranylgeranyl moiety from geranylgeranyl pyrophosphate to rhoA p21 having the CAAX motif (rhoA p21-CAAX) but not to rhoA p21 lacking the AAX portion. rhoA p21 GGT was separated from the previously reported farnesyltransferase for ras p21s (ras p21 FT) by column chromatographies and did not geranylgeranylate or farnesylate c-Ha-ras p21-CAAX. ras p21 FT did not geranylgeranylate or farnesylate rhoA p21-CAAX. These results indicate that rhoA p21 GGT distinct from ras p21 FT is present in bovine brain cytosol.
authors.affiliation: Department of Biochemistry, Kobe University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Kawata
authors.forename: M
authors.initials: M
authors.lastname: Katayama
authors.forename: H
authors.initials: H
authors.lastname: Horiuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Kita
authors.forename: Y
authors.initials: Y
authors.lastname: Takai
mesh_terms: D019883:Alkyl and Aryl Transferases; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D003600:Cytosol / Q000201:enzymology; D019204:GTP-Binding Proteins / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism*; D013379:Substrate Specificity; D014166:Transferases / Q000302:isolation & purification / Q000378:metabolism*; D020742:rhoA GTP-Binding Protein
chemical_list: D014166:Transferases; D019883:Alkyl and Aryl Transferases; C064911:p21(ras) farnesyl-protein transferase; C068462:rhoA p21 geranylgeranyltransferase; D019204:GTP-Binding Proteins; D016283:Proto-Oncogene Proteins p21(ras); D020742:rhoA GTP-Binding Protein