c-FOS-like immunoreactivity in rat brainstem neurons following noxious chemical stimulation of the nasal mucosa.
It has previously been shown that noxious and non-noxious peripheral stimuli induce c-fos expression in spinal dorsal horn neurons. In the present study we have examined the expression of c-fos in brainstem neurons following noxious chemical stimulation of the respiratory region of the nasal mucosa. In urethane-anaesthetized rats we injected mustard oil or applied CO2 pulses to the right nasal cavity. In control animals we applied paraffin oil or a continuous flow of air. A further group of control animals was anaesthetized and not subjected to any experimental treatment. Two hours after the first stimulus the rats were perfused with 4% phosphate-buffered paraformaldehyde. Brainstem sections were incubated with primary antiserum against the FOS protein and processed according to the ABC method. Only the mustard oil-treated rats had obvious signs of rhinitis and displayed FOS-positive cells in laminae I and II of the subnucleus caudalis and in the subnucleus interpolaris of the trigeminal brainstem nuclear complex as well as in the medullary lateral reticular nucleus. These areas are known to be involved in the processing of nociceptive information. Although CO2 pulses applied to the nasal mucosa are known to evoke pain sensations in man we did not observe any FOS-positive neurons in trigeminal and reticular brainstem areas of CO2-treated rats. This lack of c-fos expression probably results from the fact that unlike mustard oil, CO2 did not induce any apparent inflammatory reactions. In all animals c-fos expression was found in the nucleus of the solitary tract and in the area postrema. Staining in these areas might partly result from factors related to anaesthesia, changed respiration parameters and stress. Since the mustard oil-treated rats displayed the highest levels of immunoreactivity in the nucleus of the solitary tract and in the area postrema, additional effects specifically related to nociceptive input are very likely.
Anton
F
F
Institut für Physiologie und Biokybernetik, Erlangen, F.R.G.
Herdegen
T
T
Peppel
P
P
Leah
J D
JD
D000344:Afferent Pathways / Q000502:physiology*; D000818:Animals; D001933:Brain Stem / Q000502:physiology*; D002245:Carbon Dioxide / Q000494:pharmacology; D017879:Isothiocyanates; D008297:Male; D009297:Nasal Mucosa / Q000294:innervation*; D009474:Neurons / Q000502:physiology*; D009619:Nociceptors / Q000187:drug effects / Q000502:physiology; D011505:Protein-Tyrosine Kinases / Q000032:analysis; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D016760:Proto-Oncogene Proteins c-fos; D011519:Proto-Oncogenes; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013861:Thiocyanates / Q000494:pharmacology; D014278:Trigeminal Nuclei / Q000187:drug effects / Q000502:physiology*
D017879:Isothiocyanates; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D013861:Thiocyanates; D002245:Carbon Dioxide; C004471:allyl isothiocyanate; D011505:Protein-Tyrosine Kinases