Phosrestins I and II: arrestin homologs which undergo differential light-induced phosphorylation in the Drosophila photoreceptor in vivo.
The 49-kDa phosphoprotein gene and Dmarrestin gene encode two distinct arrestin homologs in the Drosophila photoreceptor. We find that two DNA fragments representing the Dmarrestin gene hybrid-selected a mRNA the in vitro translation of which produced a protein corresponding to the 39-kDa phosphoprotein previously reported by us. We propose to name these phosphorylated homologs of arrestin phosrestin I (49-kDa protein) and phosrestin II (39-kDa protein or the Dmarrestin gene product). We find that phosrestins I and II follow different time courses of phosphorylation in vivo; in the time period (approximate seconds) during which 43% of phosrestin I became phosphorylated, the phosphorylated state of phosrestin II remained unchanged from that of the nonilluminated flies. These results indicate that phosrestins I and II probably occupy different functional roles in the Drosophila photoreceptor.
Matsumoto
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H
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City 73190.
Yamada
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T
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000235:genetics*; D019393:Arrestin; D019390:Arrestins; D001483:Base Sequence; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005136:Eye Proteins / Q000235:genetics*; D007301:Insect Hormones / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008027:Light; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015345:Oligonucleotide Probes; D010750:Phosphoproteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010766:Phosphorylation; D010786:Photoreceptor Cells / Q000502:physiology*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
D000941:Antigens; C504665:Arr1 protein, Drosophila; D019393:Arrestin; D019390:Arrestins; D029721:Drosophila Proteins; D005136:Eye Proteins; D007301:Insect Hormones; D008565:Membrane Proteins; D015345:Oligonucleotide Probes; D010750:Phosphoproteins; D012333:RNA, Messenger