Quality control testing of surfaces for mammalian cell culture using cells propagated in a protein-free medium.
Mouse NCTC clone 929 L (L-929) cells were propagated continuously for 3 years as monolayers in a protein-free chemically-defined medium. These cells, designated L-929-WS, were used for quality control testing of the surfaces of commercially available cell culture plastic flasks. Differences in attachment and saturation density of L-929-WS cells in a protein-free culture medium were taken to define various levels of quality of the culture vessels tested. The rate of attachment and growth of L-929-WS cells on a surface of a given quality correlated directly with that of human embryonal fibroblasts and embryonal epithelial cells grown in a serum-free medium supplemented with growth factors and hormones. L-929-WS cells propagated continuously in a protein-free medium provide a simple and sensitive assay system for more general quality control testing of surfaces used for the culture of monolayer cells.
Cinatl
J
J
Centre of Hygiene, Department of Virology, Clinics of the J.W. Goethe University Frankfurt a. M., Federal Republic of Germany.
Cinatl
J
J
Rabenau
H
H
Ahn
J W
JW
Doerr
H W
HW
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002999:Clone Cells / Q000166:cytology*; D003470:Culture Media; D003584:Cytological Techniques / Q000295:instrumentation / Q000592:standards*; D006801:Humans; D011786:Quality Control; D013499:Surface Properties
D003470:Culture Media