An SfiI restriction map of the Bacillus subtilis 168 genome.
A restriction map of 24 SfiI (GGCCN4/NGGCC) restriction fragments has been constructed for the Bacillus subtilis genome. The combined sizes of the fragments indicate a genome size of approx. 4.2 Mb. The SfiI fragments range in size from 7-730 kb. Genetic markers have been located on 19 of the SfiI fragments, and 69 genetic markers have been assigned to the SfiI restriction map.
Amjad
M
M
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140.
Castro
J M
JM
Sandoval
H
H
Wu
J J
JJ
Yang
M
M
Henner
D J
DJ
Piggot
P J
PJ
D001412:Bacillus subtilis / Q000235:genetics*; D015139:Blotting, Southern; D015252:Deoxyribonucleases, Type II Site-Specific; D004586:Electrophoresis; D005798:Genes, Bacterial; D005819:Genetic Markers; D015183:Restriction Mapping
D005819:Genetic Markers; C041749:endodeoxyribonuclease SfiI; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases