Reduction of mono(ADP-ribosyl)ation of 20 kDa protein with maturation in rat testis: involvement of guanine nucleotides.
When the homogenate prepared from immature rat testes was incubated with [32P]NAD, several proteins (90, 39 and 20 kDa) were ADP-ribosylated in the absence of bacterial toxins. This observation suggested the existence of an endogenous ADP-ribosyltransferase and substrates. The data that the digested product by phosphodiesterase of ADP-ribosylated 20 kDa protein was 5'-AMP suggested that 20 kDa protein was mono(ADP-ribosyl)ated. In addition, the mono(ADP-ribosyl)ation of 20 kDa protein was enhanced by guanine nucleotides such as GTP, GDP and GTP[gamma S], and decreased by the concentrations of 10 mM Mg2+. In contrast, the incorporation of ADP-ribose moiety from NAD to both 90 and 39 kDa proteins was not changed by guanine nucleotides. On the other hand, mono(ADP-ribosyl)ation of 20 kDa protein was not observed in the homogenate prepared from other tissues of the same rats. Furthermore, we found that mono(ADP-ribosyl)ation of 20 kDa protein was decreased with the maturation of the rats and that an endogenous mono(ADP-ribosyl)transferase and 20 kDa protein were located in the nuclei.
Yamashita
A
A
Department of Physiological Chemistry, Hiroshima University School of Medicine, Japan.
Sato
E
E
Yasuda
H
H
Kurokawa
T
T
Ishibashi
S
S
D036002:ADP Ribose Transferases / Q000378:metabolism; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000375:Aging / Q000378:metabolism; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism; D006150:Guanine Nucleotides / Q000502:physiology*; D008297:Male; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009928:Organ Specificity; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000378:metabolism; D013737:Testis / Q000254:growth & development* / Q000378:metabolism
D006150:Guanine Nucleotides; D009687:Nuclear Proteins; D009243:NAD; D000246:Adenosine Diphosphate Ribose; D036002:ADP Ribose Transferases