Collagens of the chicken eggshell membranes.
An immunohistochemical analysis of the eggshell membranes shows the occurrence of type X collagen while type I collagen was not detected by using an appropriate monoclonal antibody with untreated shell membranes. A positive immuno-reaction for type I collagen was obtained after digestion of the shell membranes with pepsin. These observations indicate the possibility that type I collagen epitope was masked by type X collagen and that type X collagen may serve as an inhibitory boundary for biomineralization.
Arias
J L
JL
Department of Animal Biological Sciences, University of Chile, Santiago.
Fernandez
M S
MS
Dennis
J E
JE
Caplan
A I
AI
D000085:Acetates / Q000494:pharmacology; D019342:Acetic Acid; D000818:Animals; D000911:Antibodies, Monoclonal; D002642:Chick Embryo; D003094:Collagen / Q000032:analysis*; D004528:Egg Shell / Q000737:chemistry*; D005455:Fluorescent Antibody Technique; D006026:Glycoside Hydrolases; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D008566:Membranes / Q000737:chemistry; D008903:Minerals / Q000378:metabolism; D010434:Pepsin A / Q000494:pharmacology; D001616:beta-Galactosidase / Q000494:pharmacology
D000085:Acetates; D000911:Antibodies, Monoclonal; D008903:Minerals; D003094:Collagen; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase; D006821:Hyaluronoglucosaminidase; D010434:Pepsin A; D019342:Acetic Acid