title: Biological functions of the water-insoluble fraction of mouse seminal vesicle fluid. I. Suppression of the blastogenic response of lymphocytes.
abstract: The effects of the water-insoluble fraction of mouse seminal vesicle fluid (WIF-SVF) on lymphocytes was investigated to clarify its role in reproductive immunity. WIF-SVF inhibited the blastogenic response of T-cells to concanavalin-A (Con-A), but it did not inhibit the blastogenic response of B-cells to lipopolysaccharide (LPS). Pretreatment of splenocytes with WIF-SVF did not suppress the blastogenic response of splenocytes to Con-A when treated cells were washed prior to culture. WIF-SVF did not inhibit the proliferation of Con-A activated splenocytes, the response of listeria-immune splenocytes to listerial antigen, or the proliferation of IL 2-dependent HT-2 cells, or the growth of tumour cells (Yac 1 cells, Ehrlich ascites carcinoma cells, EL-4 cells). A listerial antigen-specific immune response was not induced after mice were immunized with both listerial antigen and WIF-SVF. WIF-SVF is mainly composed of protein and its suppressive activity was enhanced by heating at 100 degrees C. These results suggest that WIF-SVF inhibits the responsiveness of T-cells to antigens or mitogens non-specifically at the initial stage.
authors.affiliation: Department of Bacteriology, Nara Medical University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Emoto
authors.forename: F
authors.initials: F
authors.lastname: Nishikawa
authors.forename: A
authors.initials: A
authors.lastname: Hamuro
authors.forename: D
authors.initials: D
authors.lastname: Oku
authors.forename: E
authors.initials: E
authors.lastname: Kita
authors.forename: S
authors.initials: S
authors.lastname: Kashiba
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000276:immunology; D002478:Cells, Cultured; D003208:Concanavalin A; D006358:Hot Temperature; D007108:Immune Tolerance; D007114:Immunization; D007376:Interleukin-2 / Q000378:metabolism; D008089:Listeria monocytogenes / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012669:Seminal Vesicles / Q000737:chemistry / Q000276:immunology*; D012995:Solubility; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D007376:Interleukin-2; D003208:Concanavalin A