Isolation and characterization of a temperature-sensitive conditional mutant of Escherichia coli altered for the control of phosphate-regulated proteins.
We have identified a conditional mutation which confers a pleïotropic phenotype to Escherichia coli cells: no growth at temperature higher than 36 degrees C, an altered control of the synthesis of several phosphate-regulated polypeptides (including alkaline phosphatase, sn-glycerol-3-phosphate binding protein, phosphate binding protein and outer membrane porin protein PhoE) after growth at 36 degrees C and a wild-type phenotype at 30 degrees C. This mutation was located at minute 89.5 on the E. coli chromosome in a gene we have called cpr for conditional phosphate-regulated.
Uribellarea
J L
JL
Centre de Recherche de Biochimie et de Génétique Cellulaire du CNRS, Toulouse, France.
Heyde
M
M
Portalier
R
R
D000469:Alkaline Phosphatase / Q000032:analysis; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002874:Chromosome Mapping; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005798:Genes, Bacterial; D009154:Mutation; D010710:Phosphates / Q000494:pharmacology*; D013696:Temperature; D001616:beta-Galactosidase / Q000032:analysis
D001426:Bacterial Proteins; D010710:Phosphates; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase