The expression of the Ig H chain repertoire in developing bone marrow B lineage cells.
Analyses of rearranged Ig H chain V region genes of bone marrow pre-B cells demonstrate extensive sequence diversity, particularly within the third hypervariable region (HCDR3). This diversity is constrained, however, through preferential utilization of certain D gene segments and possibly VH gene segments and a preponderance of productive rearrangements, primarily those expressing D gene segments in a preferred reading frame. The predominance of productively rearranged V genes with D regions translated in a preferred frame, is, at least in part, the consequence of selective clonal expansion encompassing at least five to six divisions subsequent to VH-D-JH rearrangement. Selection for clonal expansion appears to be dependent on recognition of the nascent H chain product of certain productively rearranged genes.
Decker
D J
DJ
Department of Immunology, Scripps Clinic & Research Foundation, La Jolla, CA 92037.
Boyle
N E
NE
Koziol
J A
JA
Klinman
N R
NR
D000916:Antibody Diversity; D001402:B-Lymphocytes / Q000166:cytology* / Q000502:physiology; D001483:Base Sequence; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002999:Clone Cells; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D006410:Hematopoiesis; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region