T cell non-MHC-restricted antigen-binding molecules secreted or associated with the cell membrane are antigenically distinct.
Some T cells produce membrane-associated or soluble molecules which bind nominal antigen specifically (TABM) and effect immunoregulation or events similar to cell-mediated hypersensitivity. We have used polyclonal antisera raised against an azobenzene arsonate (ABA)-specific TABM secreted by an ABA-specific T cell hybrid or against TNP-specific polypeptides produced by immunoregulatory T cells to identify the expression of soluble (secreted) or membrane-associated TABM. Ascites fluid or culture medium containing a T cell hybrid or T cell lines, respectively, contain TABM recognized only by an antiserum specific for the secreted T cell hybrid (ABA-specific) derived TABM. Conversely, an antiserum that recognized the TNP-specific polypeptides detected cell-membrane associated TABM but did not bind TABM secreted by the T cell hybrid or cell lines.
Cone
R E
RE
Department of Pathology, University of Connecticut Health Center, Farmington 06030.
Clark
R B
RB
Kristie
J
J
Wong
R
R
Rellahan
B L
BL
D000818:Animals; D000941:Antigens / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D066298:In Vitro Techniques; D007526:Isoelectric Point; D051379:Mice; D008970:Molecular Weight; D011485:Protein Binding; D012995:Solubility; D013491:Suppressor Factors, Immunologic / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology; D010132:p-Azobenzenearsonate / Q000276:immunology
D000941:Antigens; D002352:Carrier Proteins; D013491:Suppressor Factors, Immunologic; D014301:Trinitrobenzenes; D010132:p-Azobenzenearsonate