title: UGA can be decoded as tryptophan at low efficiency in Bacillus subtilis.
abstract: Replacement of cat-86 codon 7 or 144 with the UGA codon permitted the gene to confer chloramphenicol resistance in wild-type Bacillus subtilis. UAA replacements of the same codons resulted in a chloramphenicol-sensitive phenotype in wild-type B. subtilis and a chloramphenicol-resistant phenotype in suppressor-positive strains. N-terminal sequencing showed that UGA at codon 7 was decoded as tryptophan in wild-type cells, at an efficiency of about 6%.
authors.affiliation: Department of Biological Sciences, University of Maryland-Baltimore County, Catonsville 21228-5398.
authors.forename: P S
authors.initials: PS
authors.lastname: Lovett
authors.forename: N P
authors.initials: NP
authors.lastname: Ambulos
authors.forename: W
authors.initials: W
authors.lastname: Mulbry
authors.forename: N
authors.initials: N
authors.lastname: Noguchi
authors.forename: E J
authors.initials: EJ
authors.lastname: Rogers
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D002702:Chloramphenicol Resistance / Q000235:genetics; D002874:Chromosome Mapping; D003062:Codon / Q000235:genetics*; D013489:Suppression, Genetic; D014169:Transformation, Bacterial; D014364:Tryptophan
chemical_list: D003062:Codon; D014364:Tryptophan