title: Three-dimensional structure of recombinant human interferon-gamma.
abstract: The x-ray crystal structure of recombinant human interferon-gamma has been determined with the use of multiple-isomorphous-replacement techniques. Interferon-gamma, which is dimeric in solution, crystallizes with two dimers related by a noncrystallographic twofold axis in the asymmetric unit. The protein is primarily alpha helical, with six helices in each subunit that comprise approximately 62 percent of the structure; there is no beta sheet. The dimeric structure of human interferon-gamma is stabilized by the intertwining of helices across the subunit interface with multiple intersubunit interactions.
authors.affiliation: Department of Pharmacology, University of Alabama, Birmingham 35294.
authors.forename: S E
authors.initials: SE
authors.lastname: Ealick
authors.forename: W J
authors.initials: WJ
authors.lastname: Cook
authors.forename: S
authors.initials: S
authors.lastname: Vijay-Kumar
authors.forename: M
authors.initials: M
authors.lastname: Carson
authors.forename: T L
authors.initials: TL
authors.lastname: Nagabhushan
authors.forename: P P
authors.initials: PP
authors.lastname: Trotta
authors.forename: C E
authors.initials: CE
authors.lastname: Bugg
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D006031:Glycosylation; D006801:Humans; D007371:Interferon-gamma / Q000737:chemistry*; D046911:Macromolecular Substances; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011994:Recombinant Proteins; D012689:Sequence Homology, Nucleic Acid; D014961:X-Ray Diffraction
chemical_list: D046911:Macromolecular Substances; D011994:Recombinant Proteins; D007371:Interferon-gamma