title: Effects of cholesterol on the function and thermotropic properties of pure UDP-glucuronosyltransferase.
abstract: The effects of cholesterol on the activity and thermal properties of a pure, delipidated isoform of UDP-glucuronosyltransferase were examined after incorporation of enzyme into unilamellar bilayers of distearoylphosphatidylcholine (DSPC) or dioleoylphosphatidylcholine (DOPC). Cholesterol, in bilayers of DSPC, decreased enzyme activity and lowered the temperature (from 37 to 30 degrees C) for a reversible transition from the active form of the enzyme to a less active form. These effects could be separated from each other in that the effect on reversible inactivation of the enzyme occurred at lower concentrations of cholesterol than the effect on activity of the active form of the enzyme. In addition, cholesterol in bilayers of DSPC stabilized UDP-glucuronosyltransferase against irreversible thermal inactivation. The extent of stabilization increased with increasing concentration of cholesterol in the bilayers. The effects of cholesterol on UDP-glucuronosyltransferase depended, however, on the nature of the bilayer containing cholesterol. Cholesterol had small effects, if any, on the properties of UDP-glucuronosyltransferase in bilayers of DOPC.
authors.affiliation: Department of Medicine, Cornell University Medical College, New York, New York 10021.
authors.forename: M
authors.initials: M
authors.lastname: Rotenberg
authors.forename: D
authors.initials: D
authors.lastname: Zakim
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000737:chemistry*; D014453:Glucuronosyltransferase / Q000737:chemistry* / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008081:Liposomes; D008862:Microsomes, Liver / Q000201:enzymology; D010713:Phosphatidylcholines / Q000737:chemistry; D013552:Swine; D013696:Temperature
chemical_list: D008081:Liposomes; D010713:Phosphatidylcholines; D002784:Cholesterol; D014453:Glucuronosyltransferase