Differential effects of tumor necrosis factor on the growth and differentiation of neuroblastoma and glioma cells.
We have studied the effect of tumor necrosis factor (TNF-alpha) on transformed neural and glial-derived cell lines. TNF-alpha at physiological doses was able to arrest the growth and inhibit DNA synthesis of N103 neuroblastoma cells. This phenomenon was accompanied by a morphological cell differentiation characterized by the outgrowth of neurites. By contrast, TNF-alpha induced an increase in the growth rate of C6 glioma cells and upon cytokine addition a higher number of C6 cells were found in the S + G2 phase of the cell cycle. C6 cells did not show morphological changes under this treatment. Analogous results were obtained with IFN-gamma. These neurotrophic and mitogenic effects of TNF-alpha suggest a putative role of this cytokine in the regeneration of brain tissue upon brain injury.
Muñoz-Fernández
M A
MA
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
Armas-Portela
R
R
Díaz-Nido
J
J
Alonso
J L
JL
Fresno
M
M
Avila
J
J
D000818:Animals; D001369:Axons / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000473:pathology*; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D007371:Interferon-gamma / Q000494:pharmacology; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D004247:DNA