title: Single cell analysis of mesoderm formation in the Xenopus embryo.
abstract: We have examined the developmental specification of individual cells in the Xenopus blastula using a new in vitro culture system. Regional differences are apparent at the mid-blastula stage when animal hemisphere cells form only ectodermal cell types, while many clones from below the pigment boundary contain mesodermal cell types. A number of clones give rise to more than one differentiated cell type indicating that the initial steps of mesoderm induction are potentially reversible. Animal hemisphere cells can be induced to form mesoderm by fibroblast growth factor (FGF). Different cell types predominate at different FGF concentrations and the neighbours in this sequence are also the pairs of cell types most usually associated in mixed clones derived from the marginal zone. We propose that the specification of individual cells depends upon both the concentration of inducing factor and on stochastic intracellular events.
authors.affiliation: Department of Zoology, University of Oxford, UK.
authors.forename: S F
authors.initials: SF
authors.lastname: Godsave
authors.forename: J M
authors.initials: JM
authors.lastname: Slack
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology* / Q000648:ultrastructure; D002478:Cells, Cultured; D004627:Embryonic Induction / Q000502:physiology*; D005346:Fibroblast Growth Factors / Q000502:physiology; D008648:Mesoderm / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D008854:Microscopy, Electron; D014981:Xenopus / Q000196:embryology*
chemical_list: D005346:Fibroblast Growth Factors