Buffy coat from families of Alzheimer's disease patients produces intracytoplasmic neurofilament accumulation in hamster brain.
Buffy coat of three members from a family with Alzheimer's disease was inoculated into hamster brains. Eighteen months after the inoculation, all experimental animals were sacrificed for the neuropathological study. Hematoxylin-eosin staining showed no gross vacuolar degeneration, or neuronal loss in the cortex. The spongiform degeneration was minimum. Immunostaining with antibodies against neurofilament 200 kDa subunit protein revealed massive immuno-positive intracytoplasmic inclusion bodies within neurons of the brainstem nuclei. By electron microscopy, the intracytoplasmic inclusion body was shown to be composed of proliferated 10 nm neurofilaments. The intra-cytoplasmic neurofilament proliferation was observed with the hamsters inoculated with the buffy coat from Alzheimer's disease patients as well as an apparently normal member of the family.
Takeda
M
M
Department of Neuropsychiatry, Osaka University Medical School, Japan.
Nishimura
T
T
Kudo
T
T
Tanimukai
S
S
Tada
K
K
D000544:Alzheimer Disease / Q000097:blood*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D006224:Cricetinae; D003593:Cytoplasm / Q000378:metabolism*; D006801:Humans; D007267:Injections; D008647:Mesocricetus; D016900:Neurofilament Proteins / Q000378:metabolism*; D012016:Reference Values
D016900:Neurofilament Proteins