Effects of substrate texture and curvature on the morphology of cultured cells.
The ultrastructural characteristics of several growth matrices were examined using two cell types chosen for their distinct growth habits. Chinese hamster ovary cells and Balb-c 3T3 mouse fibroblasts were grown on flat substrates (glass, tissue culture plastic, Millipore filters) as well as spherical (glass, tissue culture plastic, cross-linked dextran) substrates. Cells were plated maintaining equal densities and growth surface area. Once the majority of the cells reached confluency, the cell's morphology on each matrix was examined using scanning electron microscopy. Digital analysis was performed on cell attachment area to compare the effect of each matrix on cell spreading. Variation in cell shape was dramatic between matrices, being most noticeable between a textured surface (filter, dextran bead) and that of a smooth (glass) surface. Even within smooth surfaces, some variation was observed. There was also an effect of matrix curvature on cell attachment area, the greatest being in the 3T3-c Balb cells, causing an overall decrease in the area of attachment between cell and matrix. The changes seen could also be related to the particular cell type used. Hamster ovary cells tended to be cylindrical and showed little effect between matrices, whereas the mouse fibroblasts, which are more flattened, showed the matrix effect to a greater degree. This study demonstrates the necessity of being aware of substrate-induced cell changes in tissue culture, where some variation in cell shape may be due to the surface on which the cells are grown as opposed to the experimental procedure.
Hogan
M E
ME
Center for Electron Optics, Michigan State University, East Lansing 48824.
Degaetano
D H
DH
Klomparens
K L
KL
D000703:Analog-Digital Conversion; D000818:Animals; D002478:Cells, Cultured / Q000648:ultrastructure*; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning
D003470:Culture Media