A rare GC mutant in horses disclosed by isoelectric focusing and subsequent immunoprinting.
An apparent incompatibility in the GC/DBP system in a mare and her colt was found after classification by polyacrylamide gel electrophoresis. Because of this observation an analysis of the equine GC/DBP system by isoelectric focusing and subsequent immunoprinting was initiated. Further GC subtypes, however, were not encountered: the three phenotypes GC F, FS and S were also delineated by this method. The GC types in the case of disputed descent were dissolved: GC S was found in the mare and GC FS in her colt as well as in another of her male offspring. It is proposed that a rare GC mutant is responsible for this observation, namely a GC S* component which is readily classified by isoelectric focusing and immunoprinting and difficult to disclose by polyacrylamide gel electrophoresis.
Cleve
H
H
Institut für Anthropologie und Humangenetik, Universität München, BRD.
Schmid
D O
DO
D000818:Animals; D005260:Female; D006736:Horses / Q000235:genetics*; D007525:Isoelectric Focusing; D008297:Male; D009154:Mutation; D010375:Pedigree; D010641:Phenotype; D014809:Vitamin D-Binding Protein / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification
D014809:Vitamin D-Binding Protein