Inhibition of thymidylate synthase by glyceraldehyde 3-phosphate.
1. A number of common metabolites which had carbonyl and/or phosphate groups were tested for their ability to alter the activity of thymidylate synthase from Lactobacillus casei. Glyceraldehyde 3-phosphate was found to be an effective inhibitor of thymidylate synthase. 2. Glyceraldehyde 3-phosphate reversibly inhibited thymidylate synthase with a K1 of 12-13 microM; the inhibition was competitive with dUMP and noncompetitive with 5,10-methylenetetrahydrofolate which is consistent with an ordered addition of substrates.
Bures
A K
AK
Department of Chemistry, Alabama Agricultural Experiment Station, Auburn University 36849-5312.
Daron
H H
HH
Aull
J L
JL
D001667:Binding, Competitive; D003856:Deoxyuracil Nucleotides / Q000494:pharmacology; D005986:Glyceraldehyde 3-Phosphate / Q000494:pharmacology*; D007700:Kinetics; D007780:Lacticaseibacillus casei / Q000201:enzymology; D013763:Tetrahydrofolates / Q000494:pharmacology; D013940:Thymidylate Synthase / Q000037:antagonists & inhibitors*
D003856:Deoxyuracil Nucleotides; D013763:Tetrahydrofolates; C013123:5,10-methylenetetrahydrofolic acid; D005986:Glyceraldehyde 3-Phosphate; C007267:2'-deoxyuridylic acid; D013940:Thymidylate Synthase