[Certain biochemical characteristics of an insulin-like substance from the bivalve mollusk Anodonta cygnea].
An insulin-like substance (ILS) was isolated from the visceral organs of the bivalve mollusc Anodonta cygnea by chromatography on a sulfocationite CU-23 and purified by reverse phase liquid chromatography. ILS was shown to be made up to several fractions with Mr ranging from 9 to 20 kDa which have identical amino acid composition but different hydrophobicity and N-terminal amino acids. It was supposed that the heterogeneity of ILS fractions is due to its genetical or posttranslational polymorphism. ILS has a low (0.02%) affinity for the mammalian insulin receptor and a low immune affinity for mammalian insulin and possesses a mitogenic activity which is commensurate with that of the epidermal growth factor. The data obtained suggest that Anodonta cygnea ILS represents a separate branch of a relatively ancient family of insulin-like hormones and growth factors responsible for metabolism and proliferation of invertebrate tissues.
Rusakov
Iu N
IuN
Bondareva
V M
VM
Karasev
V S
VS
Leĭbush
B N
BN
Barkan
R S
RS
Pertseva
M N
MN
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004247:DNA / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D007328:Insulin / Q000031:analogs & derivatives* / Q000378:metabolism; D051379:Mice; D008974:Mollusca / Q000378:metabolism*; D051381:Rats
D000596:Amino Acids; D007328:Insulin; D004247:DNA
Nekotorye biokhimicheskie kharakteristiki insulinopodobnogo veshchestvadvustvorchatogo molliuska Anodonta cygnea.