title: Comparison of acute and chronic protein-energy malnutrition on host antitumor immune mechanisms.
abstract: Protein-calorie malnutrition (PCM) is prevalent in cancer patients. However, the effect of PCM on anti-tumor immunity is unclear and critically important in an era of improving results with adoptive immunotherapy. This study examined the effect of short- and long-term PCM on tumor-specific and natural immune effector mechanisms in a murine neuroblastoma (C1300 NRB) model. A/J mice received an isocaloric 2.5% or 24% casein diet for 3 or 8 weeks before inoculation with tumor. Three weeks later lymphocytes from tumor-bearing mice were harvested for determination of cytotoxic T lymphocyte (CTL) generation and natural killer (NK) cell cytotoxicity. Both 3 and 8 weeks of PCM significantly reduced mean total body weight by 25% (p less than 0.001) and 41% (p less than 0.001), respectively, compared with regularly nourished mice. Short-term PCM did not inhibit CTL or NK cytotoxicity, whereas long-term PCM significantly diminished CTL generation (p less than 0.001) but preserved NK cytotoxic function. These results indicate that CTL development against autologous tumor, in contrast to basal NK function, is dependent on host nutritional status. Mean tumor growth, determined by tumor-weight to carcass-weight ratio, was unchanged for both short- and long-term protein-energy deprived groups compared with results in regularly nourished mice. These findings suggest that NK function is the predominant effector mechanism inhibiting C1300 NRB growth and that NK tumoricidal capacity is preserved during PCM.
authors.affiliation: Division of Surgical Oncology, University of Pennsylvania School of Medicine, Philadelphia.
authors.forename: M D
authors.initials: MD
authors.lastname: Lieberman
authors.forename: J
authors.initials: J
authors.lastname: Reynolds
authors.forename: H P
authors.initials: HP
authors.lastname: Redmond
authors.forename: P
authors.initials: P
authors.lastname: Leon
authors.forename: J
authors.initials: J
authors.lastname: Shou
authors.forename: J M
authors.initials: JM
authors.lastname: Daly
mesh_terms: D000208:Acute Disease; D000818:Animals; D001835:Body Weight; D002908:Chronic Disease; D004195:Disease Models, Animal; D007694:Killer Cells, Natural / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D009447:Neuroblastoma / Q000150:complications / Q000276:immunology* / Q000473:pathology; D009752:Nutritional Status; D011502:Protein-Energy Malnutrition / Q000150:complications / Q000276:immunology*; D011897:Random Allocation; D012709:Serum Albumin / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D012709:Serum Albumin