title: A field evaluation of the Coulter STKS.
abstract: The performance of the Coulter STKS (Coulter, Hialeah, FL) was evaluated in a busy computerized teaching hospital laboratory. The STKS was compared with a Coulter S Plus IV and manually performed 400 white blood-cell differentials. The measured blood-count parameters (i.e., white blood cells [WBCs], red blood cells [RBCs], hemoglobulin [Hb], mean corpuscular volume [MCV], and platelets [PLTs]), compared very well between the two aperture impedance-based systems; precision, linearity, and lack of carryover were excellent. The STKS WBC differential (DIFF), derived from a combination of aperture impedance, aperture conductance, and laser light scatter, also was precise; linear and carryover were insignificant. The DIFFs (n = 424) compared well to the manual WBC differentials, with r values of 0.97, 0.97, 0.73, and 0.86 for neutrophils, lymphocytes, monocytes, and eosinophils, respectively. The DIFF and Suspect Flagging system produced 6.2% false negatives and 2.6% false positives when compared with the manual technique. These were further investigated and discussed. STKS DIFFs were stable for 18 to 24 hours in normal samples anticoagulated with K2EDTA and stored at 20 degrees C prior to analysis. Storage in the same anticoagulant at 4 degrees C and immediate aspiration preserved the DIFF analysis for considerably longer than 24 hours. These performance characteristics make the STKS a significant advancement in automated hematology.
authors.affiliation: Department of Hematology, Addenbrooke's Hospital, Cambridge, England.
authors.forename: B A
authors.initials: BA
authors.lastname: Warner
authors.forename: D M
authors.initials: DM
authors.lastname: Reardon
mesh_terms: D001322:Autoanalysis / Q000592:standards / Q000706:statistics & numerical data; D001772:Blood Cell Count / Q000295:instrumentation*; D001793:Blood Preservation; D004492:Edetic Acid; D004804:Eosinophils / Q000166:cytology; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005189:False Positive Reactions; D006801:Humans; D007958:Leukocyte Count / Q000295:instrumentation; D008214:Lymphocytes / Q000166:cytology; D009000:Monocytes / Q000166:cytology; D009504:Neutrophils / Q000166:cytology; D011786:Quality Control; D012044:Regression Analysis
chemical_list: D004492:Edetic Acid