title: Changes in organization and axonal transport of cytoskeletal proteins during regeneration.
abstract: Changes in solubility and transport rate of cytoskeletal proteins during regeneration were studied in the motor fibers of the rat sciatic nerve. Nerves were injured by freezing at the midthigh level either 1-2 weeks before (experiment I) or 1 week after radioactive labeling of the spinal cord with L-[35S]methionine (experiment II). Labeled proteins in 6-mm consecutive segments of the nerve 2 weeks after labeling were analyzed following fractionation into soluble and insoluble populations with 1% Triton at 4 degrees C. When axonal transport of newly synthesized cytoskeleton was examined in the regenerating nerve in experiment I, a new faster component enriched in soluble tubulin and actin was observed that was not present in the control nerve. The rate of the slower main component containing most of the insoluble tubulin and actin together with neurofilament proteins was not affected. A smaller but significant peak of radioactivity enriched in soluble tubulin and actin was also detected ahead of the main peak when the response of the preexisting cytoskeleton was examined in experiment II. It is thus concluded that during regeneration changes in the organization take place in both the newly synthesized and the preexisting axonal cytoskeleton, resulting in a selective acceleration in rate of transport of soluble tubulin and actin.
authors.affiliation: Department of Molecular and Cellular Neurobiology, Gunma University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tashiro
authors.forename: Y
authors.initials: Y
authors.lastname: Komiya
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001369:Axons / Q000378:metabolism*; D001692:Biological Transport; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008297:Male; D009416:Nerve Regeneration; D016900:Neurofilament Proteins; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000293:injuries / Q000378:metabolism / Q000648:ultrastructure; D014404:Tubulin / Q000378:metabolism
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D014404:Tubulin