Human chromosome 16 encodes a factor involved in induction of class II major histocompatibility antigens by interferon gamma.
Interferon gamma (IFN-gamma) induces expression of class II major histocompatibility complex (MHC)-encoded antigens in immunocompetent cells. To gain further insight into the mechanism of this induction, we prepared somatic cell hybrids between different human cell lines and a murine cell line, RAG, that does not express murine class II MHC antigens before or after treatment with murine IFN-gamma. Some of the resulting cell hybrids express murine class II MHC antigens when treated with murine IFN-gamma. This inducible phenotype is correlated with the presence of human chromosome 16. It has been shown previously that the induction of class I MHC antigens by human IFN-gamma in human-rodent hybrids requires the presence of species-specific factors encoded by chromosome 6, which bears the gene for the human IFN-gamma receptor, and chromosome 21, whose product(s) is necessary for the transduction of human IFN-gamma signals. In this report, we show that the induction of murine class II MHC antigens by human IFN-gamma in the human-RAG cell hybrids requires, likewise, the presence of human chromosomes 6 and 21, in addition to chromosome 16. In some of these hybrids, when all three of these human chromosomes were present, induction of cell-surface HLA-DR antigens was also observed. Our results demonstrate that human chromosome 16 encodes a non-species-specific factor involved in the induction of class II MHC antigens by IFN-gamma.
Bono
M R
MR
Institut National de la Santé et de la Recherche Médicale, U.276, Université Paris, France.
Alcaïde-Loridan
C
C
Couillin
P
P
Letouzé
B
B
Grisard
M C
MC
Jouin
H
H
Fellous
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D015152:Blotting, Northern; D002460:Cell Line; D002885:Chromosomes, Human, Pair 16; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D005802:Genes, MHC Class II / Q000187:drug effects*; D006681:HLA-D Antigens / Q000032:analysis / Q000235:genetics*; D015395:Histocompatibility Antigens Class I; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology / Q000276:immunology; D007371:Interferon-gamma / Q000494:pharmacology*; D051379:Mice; D011994:Recombinant Proteins; D014162:Transfection
D000911:Antibodies, Monoclonal; D006681:HLA-D Antigens; D015395:Histocompatibility Antigens Class I; D011994:Recombinant Proteins; D007371:Interferon-gamma