Cross-reactions between crude antigens of larval Taenia solium (Cysticercus cellulosae) and other helminths of pigs.
A crude antigen extract of larval Taenia solium was shown by immunodiffusion (ID) and immunoelectrophoresis (IEP) to cross-react with rabbit antisera against pig serum proteins and larval T. hydatigena, and by enzyme-linked immunosorbent assay (ELISA) with antisera against pig serum proteins, Fasciolopsis buski, larval T. hydatigena, hydatid cyst, Hymenolepis diminuta and Dipylidium caninum. Immunoblotting demonstrated that the crude antigens extract contained epitopes of pig serum proteins of 48 and 66 kDa. The crude extract also contained a subunit of antigen B (95 kDa) which was also found in T. hydatigena and H. diminuta. Immunoperoxidase and indirect immunofluorescence studies showed that cross-reacting antigens were distributed mainly on the tegument of T. solium.
Cheng
R W
RW
Department of Zoology, University of Hong Kong.
Ko
R C
RC
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D001798:Blood Proteins / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006376:Helminths / Q000276:immunology*; D015151:Immunoblotting; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007124:Immunoenzyme Techniques; D007814:Larva / Q000276:immunology; D013552:Swine; D013621:Taenia / Q000276:immunology*
D000947:Antigens, Helminth; D001798:Blood Proteins