Activation of class II gene transcription by regulatory factors is potentiated by a novel activity.
A novel activity (USA) stimulated activator-dependent transcription in a reconstituted system in conjunction with natural TFIID, resulting in 10- to 50-fold levels of induction by regulatory factors. USA mediated a modest induction by USF in conjunction with either recombinant human TFIID, intact yeast TFIID, or the evolutionarily conserved C-terminal portion of yeast TFIID. Upon further purification, USA was resolved into two components that had opposite effects on core promoter activity and that in combination potentiated activator function. Gel mobility shift experiments indicated physical interactions between the inhibitory activity and TFIID, suggesting that the additional components (cofactors) associate with the preinitiation complex both to reduce promoter activity in the absence and to increase promoter activity in the presence of transcriptional activators.
Meisterernst
M
M
Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.
Roy
A L
AL
Lieu
H M
HM
Roeder
R G
RG
D000260:Adenoviruses, Human / Q000235:genetics; D004268:DNA-Binding Proteins / Q000502:physiology; D005786:Gene Expression Regulation; D015497:HIV-1 / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D004268:DNA-Binding Proteins; D014157:Transcription Factors