Increment of alpha B-crystallin mRNA in the brain of patient with infantile type Alexander's disease.
To estimate the expression level of alpha B-crystallin in the brain of infantile type Alexander's disease, the amounts of protein and mRNA of alpha B-crystallin were measured by enzyme immunoassay (EIA) and Northern blot analysis, respectively, in the brain of patient and controls, and in the tissues from glioblastoma and astrocytoma. The alpha B-crystallin protein in the brain of patient was remarkably increased as compared with those of controls. The amount of alpha B-crystallin mRNA of patient was increased about 7-fold compared to the mean value of the control group and higher than that of glioblastoma tissue. These data suggest that increment of alpha B-crystallin mRNA in astrocytes leads to the overexpression of this protein and may be one of the main causes of infantile type Alexander's disease.
Ochi
N
N
Department of Pediatrics, Nagoya University School of Medicine, Japan.
Kobayashi
K
K
Maehara
M
M
Nakayama
A
A
Negoro
T
T
Shinohara
H
H
Watanabe
K
K
Nagatsu
T
T
Kato
K
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D001254:Astrocytoma / Q000378:metabolism; D001483:Base Sequence; D015152:Blotting, Northern; D001927:Brain Diseases / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D003459:Crystallins / Q000378:metabolism*; D015342:DNA Probes; D004802:Eosinophilia / Q000378:metabolism*; D005910:Glioma / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism*
D003459:Crystallins; D015342:DNA Probes; D012333:RNA, Messenger