Kinetic determination of talin-actin binding.
Smooth muscle talin prepared from chicken gizzard binds to skeletal muscle actin in vitro. The stoichiometry of 1:3 for talin:fluorescent labelled G-actin was confirmed by steady state titration and viscosity measurements under non-polymerizing conditions. The binding constant (Kd) of talin and G-actin was determined by continuous fluorescence titration and gave a value of approx 0.3 microM. The association rate constant of talin and fluorescent labelled G-actin of approx 7 x 10(6) M-1 x s-1 was ascertained by the stopped flow method; the dissociation rate constant was calculated at approx 2-3 s-1.
Goldmann
W H
WH
Dept. of Biophysics, Technical University of Munich, Garching, FRG.
Isenberg
G
G
D000199:Actins / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D003598:Cytoskeletal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D005895:Gizzard, Avian / Q000378:metabolism; D007700:Kinetics; D008433:Mathematics; D008565:Membrane Proteins / Q000378:metabolism; D009130:Muscle, Smooth / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D011485:Protein Binding; D011817:Rabbits; D016608:Talin; D014783:Viscosity
D000199:Actins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D016608:Talin