Elongation factor 2 from Artemia salina embryos and its affinity for ribosomes.
Crude extracts from Artemia salina undeveloped embryos do not contain detectable elongation-factor-2 (EF2) kinase and endogenous ADP-ribosylating activities. Accordingly, EF2 purified from this source is an enzyme relatively free from phosphorylated and ADP-ribosylated forms. Endogenous ADP-ribosyltransferase activity appears only after purification of EF2. The affinities of EF2 and of ADP-ribosyl-EF2 for ribosomes from A. salina undeveloped embryos have been calculated by measuring the ability of the factors to inhibit the N-glycosidase activity of ricin on ribosomes.
Zamboni
M
M
Dipartimento di Patologia sperimentale dell'Università di Bologna, Italy.
Brigotti
M
M
Montanaro
L
L
Sperti
S
S
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000818:Animals; D001156:Artemia / Q000201:enzymology; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D004591:Electrophoresis, Polyacrylamide Gel; D054736:Elongation Factor 2 Kinase; D005775:Gastrula / Q000201:enzymology; D007526:Isoelectric Point; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000302:isolation & purification / Q000378:metabolism*; D010766:Phosphorylation; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D012270:Ribosomes / Q000378:metabolism*; D012685:Sepharose / Q000031:analogs & derivatives / Q000378:metabolism
D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; C037405:heparin-sepharose; D000246:Adenosine Diphosphate Ribose; D012685:Sepharose; D011065:Poly(ADP-ribose) Polymerases; D011494:Protein Kinases; C517691:Eef2k protein, rat; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D054736:Elongation Factor 2 Kinase