title: Quantification of plasma phenylethylamine by combined gas chromatography/electron capture negative ion mass spectrometry of the N-acetyl-N-pentafluorobenzoyl derivative.
abstract: An ultrasensitive method capable of detection and quantification of beta-phenylethylamine in 1 ml of human plasma has been developed using gas chromatography/electron capture negative ion mass spectrometry. Phenylethylamine and tetra-deutero phenylethylamine internal standard in plasma were acetylated, extracted into organic solvent and then further acylated with pentafluorobenzoyl chloride. The N-acetyl-N-pentafluorobenzoyl-phenylethylamines were detected by high-resolution single ion monitoring of the molecular ions. Normal plasma levels were found to be 41.5 +/- 10.7 pg ml-1, in accordance with results of a previous high-performance liquid chromatographic method.
authors.affiliation: Neuropsychiatric Research Unit, University of Saskatchewan, Saskatoon, Canada.
authors.forename: D A
authors.initials: DA
authors.lastname: Durden
authors.forename: B A
authors.initials: BA
authors.lastname: Davis
authors.forename: A A
authors.initials: AA
authors.lastname: Boulton
mesh_terms: D002849:Chromatography, Gas; D006801:Humans; D066298:In Vitro Techniques; D013058:Mass Spectrometry; D010627:Phenethylamines / Q000097:blood*; D012016:Reference Values
chemical_list: D010627:Phenethylamines; C029261:phenethylamine