A limited number of Bacillus subtilis strains carry a tetracycline-resistance determinant at a site close to the origin of replication.
Several strains of Bacillus subtilis, e.g., 168 derivatives and R, were found to carry a single copy of a tetracycline-resistance (TcR) determinant (named tetBS908) at a site close to the origin of replication on the chromosome. This gene is highly homologous (80% identical) to the TcR determinant of plasmids widely dispersed among aerobic spore-forming bacilli. B. subtilis RM125 (168 strain) transformants which carry a varying number of tetBS908 sequences in a tandem array on the chromosome were constructed and examined for their TcR level. A nearly proportional relationship between the TcR level and copy number of tetBS908 existed.
Amano
H
H
Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.
Ives
C L
CL
Bott
K F
KF
Shishido
K
K
D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004261:DNA Replication; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011815:R Factors; D015183:Restriction Mapping; D013753:Tetracycline Resistance / Q000235:genetics*