Activated macrophages in highly irradiated cercariae-induced immunity to Schistosoma japonicum in rats.
The results of studies on the schistosomulicidal activity of activated peritoneal and alveolar macrophages (pM phi and aM phi) from rats immunized with highly irradiated (50 krad.) Schistosoma japonicum cercariae are reported. The authors have examined the activation of these macrophages in terms of spreading, adhesion and ingestion of sheep erythrocytes and pinocytosis of horse-radish peroxidase. Using three criteria, peritoneal macrophages and alveolar macrophages from immunized rats and from rats intraperitoneally injected with BCG were significantly more active than those from normal rats or rats stimulated with 10% proteose-peptone or 1% sodium thioglycolate. A significantly higher percentage of adhesion and ingestion was obtained with the sheep erythrocytes that were co-opsonized by heat-inactivated rat anti-sheep erythrocyte serum and fresh normal rat serum. Schistosomulicidal effects were observed with macrophages from irradiated cercariae-immunized rats in two activation systems: in vitro activation in the presence of macrophage-activating factor (MAF), and in vivo activation by the intraperitoneal challenge with sonicated cercarial antigens.
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Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences.
Xu
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D000818:Animals; D002448:Cell Adhesion; D003602:Cytotoxicity, Immunologic; D005260:Female; D006790:Host-Parasite Interactions; D007814:Larva / Q000276:immunology / Q000528:radiation effects; D008262:Macrophage Activation; D016215:Macrophage-Activating Factors / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology*; D010529:Peritoneal Cavity; D010587:Phagocytosis; D010873:Pinocytosis; D011650:Pulmonary Alveoli; D051381:Rats; D011919:Rats, Inbred Strains; D012549:Schistosoma japonicum / Q000276:immunology* / Q000528:radiation effects; D012554:Schistosomiasis japonica / Q000276:immunology*
D016215:Macrophage-Activating Factors