Cloning and sequencing of cDNAs encoding a pathogen-induced putative peroxidase of wheat (Triticum aestivum L.).
We report here the complete amino acid sequence of a pathogen-induced putative peroxidase from wheat (Triticum aestivum L.) as deduced from cDNA clones representing mRNA from leaves infected with the powdery mildew fungus Erysiphe graminis. The protein consists of 312 amino acids, of which the first 22 form a putative signal sequence, and has a calculated pI of 5.7. Sequence comparison revealed that the putative wheat peroxidase is most similar to the turnip (Brassica rapa) peroxidase, with which it shares 57% identical and 13% conserved amino acids.
Rebmann
G
G
Institute for Plant Biology, University of Zürich, Switzerland.
Hertig
C
C
Bull
J
J
Mauch
F
F
Dudler
R
R
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000502:physiology; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D015698:Genomic Library; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics*; D010935:Plant Diseases; D010940:Plant Proteins / Q000235:genetics*; D016415:Sequence Alignment; D014908:Triticum / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology
D010940:Plant Proteins; D004247:DNA; D010544:Peroxidases; C070865:wheat peroxidase