Determination of serum apolipoprotein B by competitive enzyme-linked immunoassay.
An enzyme-linked immunoassay was used to determine apolipoprotein B (apoB) in serum samples from 104 healthy adults aged 18-26 years. The method was compared to radial immunodiffusion (RID). Mean apoB values were 103 +/- 30 mg/dl and 105 +/- 31 mg/dl respectively. The correlation coefficient for apoB between the two methods was r = 0.70, p less than 0.001; the correlation coefficient between LDL-cholesterol and apoB measured by the ELISA was r = 0.73, p less than 0.001. This ELISA technique seems to be an appropriate method for measuring apoB in plasma, in the clinical laboratory and for detecting individuals with hyperapobetalipoproteinaemia.
Reisinger
H
H
Department of Paediatrics, University of Vienna, Austria.
Strobl
W
W
Hubrich
B
B
Widhalm
K
K
D000293:Adolescent; D000328:Adult; D001055:Apolipoproteins B / Q000097:blood*; D001667:Binding, Competitive; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000706:statistics & numerical data; D006801:Humans; D005779:Immunodiffusion; D008297:Male; D012016:Reference Values
D001055:Apolipoproteins B; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL