The HIV core protein p24 inhibits interferon-gamma-induced increase of HLA-DR and cytochrome b heavy chain mRNA levels in the human monocyte-like cell line THP1.
Cells from the human monocytic cell-line THP1 were incubated prior to activation with IFN-gamma or LPS with varying amounts of p24, the main product of the HIV gag gene and the major component of the virus core. The IFN-gamma-dependent increase of mRNA for HLA-DR and for the heavy chain of cytochrome b was markedly decreased by p24 but not by gp120. This effect was abrogated by anti-p24 antibodies. On the other hand, preincubation of THP1 cells with p24 did not affect the accumulation of the LPS-dependent mRNA for TNF alpha and IL1-beta. These results indicate that p24 at concentrations similar to those found in the serum of HIV-infected individuals specifically affects IFN-gamma-induced activation markers.
Nong
Y
Y
Department of Rheumatology and Immunology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115.
Kandil
O
O
Tobin
E H
EH
Rose
R M
RM
Remold
H G
HG
D015152:Blotting, Northern; D002460:Cell Line; D003573:Cytochrome b Group / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D015683:Gene Products, gag / Q000494:pharmacology*; D016655:HIV Core Protein p24; D015497:HIV-1 / Q000276:immunology*; D006684:HLA-DR Antigens / Q000235:genetics*; D006801:Humans; D007371:Interferon-gamma / Q000037:antagonists & inhibitors*; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation; D009000:Monocytes / Q000382:microbiology / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D014758:Viral Core Proteins / Q000494:pharmacology*
D003573:Cytochrome b Group; D015683:Gene Products, gag; D016655:HIV Core Protein p24; D006684:HLA-DR Antigens; D007375:Interleukin-1; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D014758:Viral Core Proteins; D007371:Interferon-gamma