title: Enzyme-linked immunosorbent assay using monoclonal antibodies for identification of mycobacteria from early cultures.
abstract: A simple enzyme-linked immunosorbent assay (ELISA) for the identification of cultured mycobacteria belonging to the Mycobacterium tuberculosis complex, the Mycobacterium avium complex, and Mycobacterium kansasii has been developed (R. Schöningh, C. P. H. J. Verstijnen, S. Kuijper, and A. H. J. Kolk. J. Clin. Microbiol. 28:708-713, 1990). The test for the routine identification of cultured mycobacteria was introduced in five clinical laboratories located in Tanzania, Thailand, Vietnam, and The Netherlands. The ELISA can be conducted without an ELISA reader since the test can be read visually. The results of identification of 255 strains of the M. tuberculosis complex by microbiological means and by ELISA were compared; the specificity and the sensitivity were 100%. For the M. avium complex, the specificity was 100% and the sensitivity was 64%. All 26 M. kansasii strains tested could be identified as M. kansasii. The ELISA described here proved to be useful in both well- and modestly equipped laboratories and may replace the microbiological method of identification of M. tuberculosis and M. kansasii.
authors.affiliation: Laboratory of Tropical Hygiene, Royal Tropical Institute, Amsterdam, The Netherlands.
authors.forename: C P
authors.initials: CP
authors.lastname: Verstijnen
authors.forename: H M
authors.initials: HM
authors.lastname: Ly
authors.forename: K
authors.initials: K
authors.lastname: Polman
authors.forename: C
authors.initials: C
authors.lastname: Richter
authors.forename: S P
authors.initials: SP
authors.lastname: Smits
authors.forename: S Y
authors.initials: SY
authors.lastname: Maselle
authors.forename: P
authors.initials: P
authors.lastname: Peerbooms
authors.forename: D
authors.initials: D
authors.lastname: Rienthong
authors.forename: N
authors.initials: N
authors.lastname: Montreewasuwat
authors.forename: S
authors.initials: S
authors.lastname: Koanjanart
mesh_terms: D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D006801:Humans; D009161:Mycobacterium / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000175:diagnosis; D015269:Mycobacterium avium Complex / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification; D009170:Nontuberculous Mycobacteria / Q000302:isolation & purification; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal