The separation of chondroitin sulfate disaccharides and hyaluronan oligosaccharides by capillary zone electrophoresis.
We have developed techniques for the separation of unsulfated (2-acetamido-2-deoxy-3-O-(4-deoxy-alpha-L-threo- hex-4-enopyranosyluronicacid)-D-galactose and -D-glucose), monosulfated (2-acetamido-2-deoxy-3- O-(4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranosyluronic acid)-D-galactose and 2-acetamido-2-deoxy-3-O-(4-deoxy-alpha-L-threo-hex- 4-enopyranosyluronic acid)-4-sulfo-D-galactose and -6-sulfo-D-galactose),disulfated (2-acetamido-2-deoxy-3-O-(4-deoxy-2-O-sulfo-alpha-L-threo-hex-4- enopyranosyluronic acid)-4-sulfo-D-galactose and -6-sulfo-D-galactose and 2-acet-amido-2-deoxy-3-O-(4-deoxy-alpha-L-threo-hex-4-enopy- ranosyluronic acid)-4,6-di-O-sulfo-D-galactose), and trisulfated (2-acetamido-2-deoxy-3-O-(4-deoxy-2-O- sulfo-alpha-L-threo-hex-4-enopyranosyluronic acid)-4,6-di-O-sulfo-D-galactose) isomers of chondroitin using capillary zone electrophoresis. In addition, it is possible to separate oligomers of hyaluronan by similar protocols. These techniques represent a rapid, sensitive, and reproducible technique for the assay of these molecules from digests of connective tissues.
Carney
S L
SL
Eli Lilly and Co., Lilly Research Centre Ltd., Windlesham, Surrey, United Kingdom.
Osborne
D J
DJ
D001888:Boric Acids / Q000494:pharmacology; D001896:Boron Compounds; D001897:Boronic Acids; D002021:Buffers; D002240:Carbohydrate Sequence; D002809:Chondroitin Sulfates / Q000737:chemistry*; D004187:Disaccharides / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods; D006820:Hyaluronic Acid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000302:isolation & purification*; D009994:Osmolar Concentration; D013464:Sulfuric Acids
D001888:Boric Acids; D001896:Boron Compounds; D001897:Boronic Acids; D002021:Buffers; D004187:Disaccharides; D009844:Oligosaccharides; D013464:Sulfuric Acids; C030433:n-butylboronic acid; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; C010686:benzeneboronic acid