Determination of morphine and 6-acetylmorphine in plasma by high-performance liquid chromatography with fluorescence detection.
A method is described for the simultaneous determination of morphine and 6-acetylmorphine in small volumes of human plasma by normal-phase high-performance liquid chromatography using solid-phase extraction, dansyl derivatisation and fluorescence detection. The lower limits of quantitation in a 0.1-ml plasma sample are 10 ng/ml for morphine and 25 ng/ml for 6-acetylmorphine. The method has been applied to determine concentrations of morphine and 6-acetylmorphine in plasma samples from premature babies administered an intravenous infusion of diamorphine.
Barrett
D A
DA
Department of Pharmaceutical Sciences, University Park, University of Nottingham, U.K.
Shaw
P N
PN
Davis
S S
SS
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003619:Dansyl Compounds; D004355:Drug Stability; D003932:Heroin / Q000097:blood / Q000627:therapeutic use; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature / Q000097:blood; D008832:Microchemistry; D009020:Morphine / Q000097:blood*; D009022:Morphine Derivatives / Q000097:blood*
D003619:Dansyl Compounds; D009022:Morphine Derivatives; D003932:Heroin; D009020:Morphine; C026979:6-O-monoacetylmorphine