Analysis of a Corynebacterium glutamicum hom gene coding for a feedback-resistant homoserine dehydrogenase.
From a Corynebacterium glutamicum mutant possessing a homoserine dehydrogenase resistant to feedback inhibition by L-threonine, the corresponding gene (homFBR) was analyzed and compared with the wild-type hom gene. DNA fragment exchange experiments between both genes showed that a 0.23-kb region close to the 3' terminus of homFBR was responsible for deregulation. Nucleotide sequence analysis revealed a single transition from G to A in homFBR leading to replacement of glycine-378 by glutamate in the mutant homoserine dehydrogenase.
Reinscheid
D J
DJ
Institut für Biotechnologie, 1 des Forschungszentrums Jülich GmbH, Federal Republic of Germany.
Eikmanns
B J
BJ
Sahm
H
H
D000595:Amino Acid Sequence; D003352:Corynebacterium / Q000235:genetics*; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000235:genetics; D005246:Feedback; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D006715:Homoserine Dehydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D013045:Species Specificity; D013329:Structure-Activity Relationship; D013912:Threonine / Q000494:pharmacology
D004269:DNA, Bacterial; D013912:Threonine; D006715:Homoserine Dehydrogenase