Intracerebral distribution of Gp135, a new human brain glycoprotein.
The intracerebral distribution of Gp135, a new human membrane glycoprotein, defined by monoclonal antibodies, was determined by a quantitative enzyme-linked immunosorbent assay (ELISA) and the presence in different brain regions was shown by immunohistochemistry. The gray matter of frontal cortex and cerebellum were found to express this antigen in high amounts, whereas the subcortical white matter, pons and medulla oblongata displayed low levels. The presence of Gp135 was visualized in regions in which high density of neuronal dendrites and unmyelinated axons are found. Gp135 may participate in cell adhesion processes, in which cell-cell contacts and tissue integrity are fundamental.
Berglund
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Department of Medical Chemistry and Biophysics, Umeå University, Sweden.
Thornell
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Stigbrand
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001710:Biotin / Q000378:metabolism; D001921:Brain / Q000033:anatomy & histology; D001923:Brain Chemistry; D002448:Cell Adhesion; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009419:Nerve Tissue Proteins / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D001710:Biotin