title: Immunological characterization of peptidyl-glycine-alpha-amidating monooxygenases.
abstract: Antibodies to the soluble form of the copper-containing enzyme, peptidyl-glycine-alpha-amidating monooxygenase isolated from secretory granules of bovine pituitary anterior lobes were found to belong to immunoglobulin G1. The antibodies were used to study the subcellular distribution of the enzyme in this tissue, and positive tests were found only for granular and cytosol fractions. The antibodies do not crossreact with other copper-containing systems of secretory granules, such as neurocuprein and dopamine-beta-monooxygenase. It was shown that the antibodies give the crossreaction with the enzyme isolated from secretory granules of bovine pituitary anterior lobes, cardiac atria, pancreas and adrenal medulla, indicating the antigenic identity of the enzyme from secretory granules of different glands.
authors.affiliation: Institute of Biochemistry, Academy of Sciences, Armenian Republic, Yerevan, USSR.
authors.forename: S G
authors.initials: SG
authors.lastname: Sharoyan
authors.forename: R M
authors.initials: RM
authors.lastname: Nalbandyan
mesh_terms: D000313:Adrenal Medulla / Q000201:enzymology; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions; D003594:Cytoplasmic Granules / Q000201:enzymology; D006325:Heart Atria / Q000201:enzymology; D005779:Immunodiffusion; D006899:Mixed Function Oxygenases / Q000276:immunology*; D009097:Multienzyme Complexes; D010179:Pancreas / Q000201:enzymology; D010903:Pituitary Gland, Anterior / Q000201:enzymology
chemical_list: D000906:Antibodies; D009097:Multienzyme Complexes; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase