title: Differentiation of Mycobacterium tuberculosis and Mycobacterium bovis BCG by a polymerase chain reaction assay.
abstract: Mycobacterium tuberculosis and Mycobacterium bovis are closely related species which carry different numbers of the repetitive DNA element IS6110. A polymerase chain reaction assay was developed to assess the copy number of IS6110 in a strain and thereby differentiate these two important human pathogens.
authors.affiliation: Division of Bacterial Diseases, Centers for Disease Control, Atlanta GA 30333.
authors.forename: B B
authors.initials: BB
authors.lastname: Plikaytis
authors.forename: K D
authors.initials: KD
authors.lastname: Eisenach
authors.forename: J T
authors.initials: JT
authors.lastname: Crawford
authors.forename: T M
authors.initials: TM
authors.lastname: Shinnick
mesh_terms: D001483:Base Sequence; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000145:classification* / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid