title: cysB and cysE mutants of Escherichia coli K12 show increased resistance to novobiocin.
abstract: Mutations in the cysB and cysE genes of Escherichia coli K12 cause an increase in resistance to the gyrase inhibitor novobiocin but not to coumermycin, acriflavine and rifampicin. This unusual relationship was also observed among spontaneous novobiocin resistant (Novr) mutants: 10% of Novr mutants isolated on rich (LA) plates with novobiocin could not grow on minimal plates, and among those approximately half were cysB or cysE mutants. Further analyses demonstrated that cysB and cysE negative alleles neither interfere with transport of novobiocin nor affect DNA supercoiling.
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia.
authors.forename: J
authors.initials: J
authors.lastname: Rakonjac
authors.forename: M
authors.initials: M
authors.lastname: Milic
authors.forename: D J
authors.initials: DJ
authors.lastname: Savic
mesh_terms: D000123:Acetyltransferases; D000167:Acriflavine / Q000494:pharmacology; D049933:Aminocoumarins; D001426:Bacterial Proteins / Q000235:genetics*; D002470:Cell Survival / Q000187:drug effects; D003374:Coumarins / Q000494:pharmacology; D004268:DNA-Binding Proteins; D004305:Dose-Response Relationship, Drug; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D009154:Mutation; D009675:Novobiocin; D012293:Rifampin / Q000494:pharmacology; D051050:Serine O-Acetyltransferase
chemical_list: D049933:Aminocoumarins; D001426:Bacterial Proteins; D003374:Coumarins; C062886:CysB protein, Bacteria; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D009675:Novobiocin; D000167:Acriflavine; D000123:Acetyltransferases; C070785:CysE protein, E coli; D051050:Serine O-Acetyltransferase; C004628:coumermycin; D012293:Rifampin