Effect of long-term feeding of nivalenol on aflatoxin B1-initiated hepatocarcinogenesis in mice.
Nivalenol, a trichothecene, occurs widely in cereals and foods; our current two-year feeding trial has revealed no tumorigenic activity in female mice. To investigate whether dietary nivalenol modulates the development of aflatoxin B1 (AFB1)-initiated hepatocarcinogenesis, one-week old C57Bl/6 x C3H F1 mice were injected intraperitoneally with 6 mg/kg bw AFB1 and six weeks later fed diets containing 0, 6 or 12 ppm nivalenol for one year. Male mice in all three groups developed hepatocellular carcinomas and adenomas, while the incidences in females were 31% in those given AFB1 alone and 20% and 0 in those given AFB1 with 6 and 12 ppm nivalenol, respectively. These findings indicate that dietary nivalenol suppresses AFB1-initiated hepatocarcinogenesis in female mice, presumably by acting on the promotion step.
Ueno
Y
Y
Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Kobayashi
T
T
Yamamura
H
H
Kato
T
T
Tashiro
F
F
Nakamura
K
K
Ohtsubo
K
K
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens; D004032:Diet; D005260:Female; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced* / Q000517:prevention & control; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009183:Mycotoxins / Q000494:pharmacology*; D012737:Sex Factors; D014255:Trichothecenes / Q000494:pharmacology*
D000348:Aflatoxins; D002273:Carcinogens; D009183:Mycotoxins; D014255:Trichothecenes; C038405:nivalenol; D016604:Aflatoxin B1