Differential silver enhanced double labeling in immunoelectron microscopy.
A two-sided double labeling method using protein A gold was used to demonstrate the presence of two hormones within the same anterior pituitary cell granule. A single probe size was used for both section faces but one side of the grid was silver enhanced. The use of a single probe size reduced the cost of the study and eliminated the variations in labeling efficiency that result from the use of different probe sizes.
Slater
M
M
Electron Microscope Unit, Prince Henry Hospital, Little Bay, Sydney, NSW, Australia.
D005640:Follicle Stimulating Hormone / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron / Q000379:methods*; D010903:Pituitary Gland, Anterior / Q000648:ultrastructure*; D013972:Thyrotropin / Q000032:analysis
D005640:Follicle Stimulating Hormone; D013972:Thyrotropin