Tumor cytokinetic response to total parenteral nutrition in patients with head and neck cancers.
Refeeding of patients with malignant tumors may induce tumor-cell DNA synthesis. The present study was aimed at evaluating whether induction of altered cell-cycle kinetics could be induced by intravenous total parenteral nutrition (TPN) in tumor biopsies from head and neck cancers. Nine malnourished patients with squamous cell carcinoma in the head-and-neck area were investigated before and after 5-7 d of continuous TPN. Tumor biopsies were taken in both fasted and fed states for determination of 1) ornithine decarboxylase (ODC) activity, which is rate limiting for polyamine synthesis; 2) flow-cytometric-DNA-distribution measurements; and 3) the fraction of proliferating cells expressed as immunohistochemical reactivity with the monoclonal antibody Ki-67. The histopathological differentiation, the fraction of aneuploidic cells, ODC activity, and Ki-67 reactivity were not significantly related to each other, although the number of aneuploidic cells in replicative phases correlated with the number of cells expressing the Ki-67 antigen (r = 0.86, P less than 0.01). Tumor cytokinetics showed no evidence of being changed by TPN administration.
Westin
T
T
Department of Otolaryngology, Sahlgrenska Hospital, University of Göteborg, Sweden.
Stein
H
H
Niedobitek
G
G
Lundholm
K
K
Edström
S
S
D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000473:pathology*; D002453:Cell Cycle; D004171:Diploidy; D005260:Female; D005434:Flow Cytometry; D006258:Head and Neck Neoplasms / Q000150:complications / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009748:Nutrition Disorders / Q000150:complications / Q000628:therapy*; D009752:Nutritional Status; D009955:Ornithine Decarboxylase / Q000032:analysis; D010289:Parenteral Nutrition, Total / Q000009:adverse effects*
D009955:Ornithine Decarboxylase