title: Functional limits of conformation, hydrophobicity, and steric constraints in prokaryotic signal peptide cleavage regions. Wild type transport by a simple polymeric signal sequence.
abstract: These experiments examine the role of conformation, hydrophobicity, and steric constraints in the function of the prokaryotic signal peptide cleavage region. The experimental strategy involves replacement of the wild type Escherichia coli alkaline phosphatase signal peptide cleavage region with a series of idealized model sequences designed to epitomize the particular structural and physical variables under study. By analyzing model sequences whose conformations have been determined by physical studies, we have demonstrated that efficient transport does not depend on the structural preference of the cleavage region. Although previous studies based on Chou-Fasman analysis have suggested that the cleavage region forms a beta-turn which is required for transport, our results demonstrate that either a beta-turn- or alpha-helix-fostering sequence in the cleavage region functions indistinguishably from wild type. Furthermore, the presence of a proline residue between the core and cleavage region, although common in natural sequences, is not essential for export. Cleavage regions of varying hydrophobicities can support translocation across the inner membrane, but the placement of bulky residues at positions -1 and -3 upstream of the cleavage site abolishes processing and transport to the periplasm. By reducing the signal peptide to simplified, idealized segments, this study has identified a largely polymeric sequence, MKQST(L10)-(A6), that functions equivalently to the wild type alkaline phosphatase signal peptide. This work starts to provide a basis for the design of a universal prokaryotic signal peptide that incorporates all the critical physical and structural characteristics required for transport function.
authors.affiliation: Laboratory of Bioorganic Chemistry and Biochemistry, Rockefeller University, New York, New York 10021.
authors.forename: G A
authors.initials: GA
authors.lastname: Laforet
authors.forename: D A
authors.initials: DA
authors.lastname: Kendall
mesh_terms: D000595:Amino Acid Sequence; D001345:Autoradiography; D001483:Base Sequence; D001692:Biological Transport; D002458:Cell Fractionation; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009113:Muramidase; D010957:Plasmids; D011487:Protein Conformation; D021382:Protein Sorting Signals / Q000235:genetics / Q000378:metabolism*
chemical_list: D021382:Protein Sorting Signals; D004492:Edetic Acid; D009113:Muramidase; D010450:Endopeptidases