Stabilization of clathrin coated vesicles by amantadine, tromantadine and other hydrophobic amines.
Amantadine and related compounds stabilized the structure of purified pig brain clathrin coated vesicles (CCV) at biologically relevant concentrations. Incubation of purified CCV for 30 min at 25 degrees C or 37 degrees C caused the release of clathrin, as determined by a centrifugation assay, and a reduction in the number of coated vesicles, by electron microscopy. Amantadine (10 mM), tromantadine (1 mM), amidine D295 (cyclohexylcarboximidamide-(N-benzyl)hydrochloride (10 mM), chloroquine (0.1 mM) and monodansylcadaverine (10 mM) significantly reduced the extent of dissociation.
Phonphok
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Department of Microbiology and Immunology, N.E. Ohio Universities College of Medicine, Rootstown 44272.
Rosenthal
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KS
D000547:Amantadine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000588:Amines / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000378:metabolism; D002966:Clathrin / Q000378:metabolism*; D003034:Coated Pits, Cell-Membrane / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D013329:Structure-Activity Relationship; D013552:Swine
D000588:Amines; D002966:Clathrin; D000547:Amantadine; C004279:tromantadine