Applications of monoclonal antibodies and recombinant cytokines for the treatment of human colorectal and other carcinomas.
Monoclonal antibodies (MAbs) which recognize a human tumor antigen, termed tumor-associated glycoprotein-72 (TAG-72), have successfully been used to localize primary as well as metastatic colorectal tumor lesions in patients. The localization of the anti-TAG-72 MAbs has also been exploited intraoperatively using a hand-held gamma probe. That procedure, termed radioimmunoguided surgery (RIGS), has identified occult tumors which were not detected using standard external imaging techniques. In another clinical trial, interferon-gamma (IFN-gamma) was administered intraperitoneally to patients diagnosed with either gastrointestinal or ovarian carcinoma with secondary ascites. Analysis of the tumor cells isolated from the malignant ascites revealed a substantial increase in TAG-72 expression on the surface of tumor cells isolated from seven of eight patients. The results provide evidence that the combination of an anti-carcinoma MAb with the administration of a cytokine, such as IFN-gamma, may be an effective approach for the detection and subsequent treatment, of colorectal carcinoma.
Greiner
J W
JW
Laboratory of Tumor Immunology and Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
Smalley
R V
RV
Borden
E C
EC
Martin
E W
EW
Guadagni
F
F
Roselli
M
M
Schlom
J
J
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000276:immunology*; D015179:Colorectal Neoplasms / Q000000981:diagnostic imaging* / Q000601:surgery; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000000981:diagnostic imaging; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma; D007430:Intraoperative Care; D007457:Iodine Radioisotopes; D008297:Male; D010051:Ovarian Neoplasms / Q000000981:diagnostic imaging; D011877:Radionuclide Imaging; D011994:Recombinant Proteins
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D006023:Glycoproteins; D007457:Iodine Radioisotopes; D011994:Recombinant Proteins; C047052:tumor-associated antigen 72; D007371:Interferon-gamma