title: Sgs-3 chromatin structure and trans-activators: developmental and ecdysone induction of a glue enhancer-binding factor, GEBF-I, in Drosophila larvae.
abstract: The transcription of the Drosophila melanogaster 68C salivary gland glue gene Sgs-3 involves the interaction of a distal and a proximal regulatory region. These are marked in vivo by a specific chromatin structure which is established sequentially during development, starting early in embryogenesis. The distal region is characterized by a stage- and tissue-specific DNase I hypersensitive site. A stage- and tissue-specific factor, GEBF-I, binds in this region and is missing in 2B5 mutant larvae which lack Sgs-3 transcripts. This binding involves the simultaneous interaction with two distinct DNA sequences which induces conformational changes in the protein. Salivary glands acquire competence to respond to ecdysone in the mid-third larval instar, whereafter the hormone rapidly induces both the GEBF-I protein and Sgs-3 transcription.
authors.affiliation: Laboratoire de Génétique Moléculaire des Eucaryotes du Centre National de la Recherche Scientifique, Unité 184 de Biologie Moléculaire, Strasbourg, France.
authors.forename: P
authors.initials: P
authors.lastname: Georgel
authors.forename: P
authors.initials: P
authors.lastname: Ramain
authors.forename: A
authors.initials: A
authors.lastname: Giangrande
authors.forename: G
authors.initials: G
authors.lastname: Dretzen
authors.forename: G
authors.initials: G
authors.lastname: Richards
authors.forename: M
authors.initials: M
authors.lastname: Bellard
mesh_terms: D000367:Age Factors; D000818:Animals; D001483:Base Sequence; D002843:Chromatin / Q000648:ultrastructure; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development; D004440:Ecdysone / Q000494:pharmacology; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D005796:Genes; D005967:Glue Proteins, Drosophila / Q000235:genetics*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D011485:Protein Binding; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D005967:Glue Proteins, Drosophila; D009841:Oligonucleotides; D014157:Transcription Factors; D004440:Ecdysone