Sulfated O-glycoproteins secreted by guinea pig trachea in organ culture.
Organ culture of guinea pig trachea was performed in the presence of [35S]sulfate in order to characterize the sulfated glycoproteins released from the respiratory epithelium and mucosa. The sulfated macromolecules that were synthesized during a 6-h incorporation were separated by CsBr density-gradient centrifugation and gel-filtration chromatography successively. Most of the sulfated secreted macromolecules corresponded to a population of glycoproteins sensitive to reductive beta-elimination but resistant to both chondroitinase ABC and heparinase. These glycoproteins had different buoyant densities (ranging from 1.48 g/ml to 1.16 g/ml) and could be subfractionated according to molecular mass. A major part of the radioactivity was incorporated into high-molecular-mass mucins that were excluded from a Sepharose CL-2B column and did not penetrate into polyacrylamide gel in PAGE. However, a mixture of sulfated O-glycoproteins of much lower molecular mass was also characterized in addition to low amounts of chondroitin sulfate. Epithelial goblet cells are the predominant mucin-containing cells of the respiratory guinea pig trachea. Our results suggest that a wide range of sulfated O-glycoproteins are secreted by the guinea pig tracheal mucosa.
Rahmoune
H
H
Unité INSERM No. 16, Lille, France.
Rounding
H P
HP
McDonald-Gibson
W J
WJ
Lamblin
G
G
Hall
R L
RL
Roussel
P
P
D000818:Animals; D002499:Centrifugation, Density Gradient; D002808:Chondroitin Lyases / Q000378:metabolism; D002809:Chondroitin Sulfates / Q000378:metabolism; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006001:Glycoconjugates / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006026:Glycoside Hydrolases; D006168:Guinea Pigs; D019763:Heparin Lyase; D006863:Hydrogen-Ion Concentration; D008297:Male; D009092:Mucous Membrane / Q000378:metabolism; D009924:Organ Culture Techniques; D011133:Polysaccharide-Lyases / Q000378:metabolism; D014132:Trachea / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
D006001:Glycoconjugates; D006023:Glycoproteins; D002809:Chondroitin Sulfates; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase; D002808:Chondroitin Lyases; D011133:Polysaccharide-Lyases; D019763:Heparin Lyase