Sequencing of peptides containing alanine, asparagine, histidine, isoleucine and tryptophan by partial methanolysis and fast atom bombardment mass spectrometry.
Five peptides containing the amino acids alanine, asparagine, histidine, isoleucine and tryptophan were investigated by partial methanolysis and fast atom bombardment mass spectrometry in order to examine the behaviour of these amino acid residues under the conditions employed in the methanolytic step. The results obtained confirm that partial methanolysis prior to mass analysis increases considerably the content of sequence information in the mass spectra and that no secondary reactions occur in the residues of the amino acids now investigated, with the exception of the esterification of the glutamic acid carboxyl group and partial conversion of the asparagine amide group in the corresponding methyl ester.
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Dipartimento di Scienze Chimiche dell'Università, Catania, Italy.
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D000595:Amino Acid Sequence; D006851:Hydrochloric Acid; D000432:Methanol; D008969:Molecular Sequence Data; D010455:Peptides; D016339:Spectrometry, Mass, Fast Atom Bombardment
D010455:Peptides; D006851:Hydrochloric Acid; D000432:Methanol