Rapid and sensitive method for the determination of albendazole and albendazole sulphoxide in biological fluids.
A sensitive and selective reversed-phase high-performance liquid chromatographic method for the determination of albendazole and its active metabolite albendazole sulphoxide in plasma has been developed. It involves single-step extraction of plasma with dichloromethane, evaporation of the solvent and chromatography on a muBondapak phenyl column with a mobile phase of water containing 1% (v/v) triethylamine-methanol-acetonitrile (70:10:20, v/v) at pH 3.1. Run time is 12 min. The assay satisfies all of the criteria required for use in clinical pharmacokinetic studies and possesses important advantages, notably speed and expense, over current methods.
Hoaksey
P E
PE
Department of Pharmacology and Therapeutics, University of Liverpool, U.K.
Awadzi
K
K
Ward
S A
SA
Coventry
P A
PA
Orme
M L
ML
Edwards
G
G
D000328:Adult; D015766:Albendazole / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D008752:Methylene Chloride; D008875:Middle Aged; D009855:Onchocerciasis / Q000097:blood
D008752:Methylene Chloride; D015766:Albendazole; C027186:albendazole sulfoxide