title: Cloning and nucleotide sequence of the anaerobically regulated pepT gene of Salmonella typhimurium.
abstract: The anaerobically regulated pepT gene of Salmonella typhimurium has been cloned in pBR328. Strains carrying the pepT plasmid, pJG17, overproduce peptidase T by approximately 70-fold. The nucleotide sequence of a 2.5-kb region including pepT has been determined. The sequence codes for a protein of 44,855 Da, consistent with a molecular weight of approximately 46,000 for peptidase T (as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration). The N-terminal amino acid sequence of peptidase T purified from a pJG17-containing strain matches that predicted by the nucleotide sequence. A plasmid carrying an anaerobically regulated pepT::lacZ transcriptional fusion contains only 165 bp 5' to the start of translation. This region contains a sequence highly homologous to that identified in Escherichia coli as the site of action of the FNR protein, a positive regulator of anaerobic gene expression. A region of the deduced amino acid sequence of peptidase T is similar to segments of Pseudomonas carboxypeptidase G2, the E. coli peptidase encoded by the iap gene, and E. coli peptidase D.
authors.affiliation: Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
authors.forename: C G
authors.initials: CG
authors.lastname: Miller
authors.forename: J L
authors.initials: JL
authors.lastname: Miller
authors.forename: D A
authors.initials: DA
authors.lastname: Bagga
mesh_terms: D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D001616:beta-Galactosidase; D000626:Aminopeptidases; C039576:peptidase T