Determination of bovine butterfat triacylglycerols by reversed-phase liquid chromatography and gas chromatography.
Triacylglycerols (TGs) from a sample of summer butterfat (bovine milk) were analysed and fractionated by reversed-phase liquid chromatography (RPLC). Fatty acid and TG composition of eac of the 47 RPLC fractions ranging from 0.1 to 6.9% were determined by capillary gas chromatography. The data were used together to determine the quantitative composition of the molecular species of TGs. A large number of TG species, accounting for 80% of the total, could be unequivocally identified and individually determined. The combination of the chromatographic methods used proved to be a powerful and accurate approach for the determination of molecular species of TGs in a complex fat, but also a difficult and time-consuming task.
Maniongui
C
C
Laboratoire de Physiologie Animale et de la Nutrition, Faculte des Sciences Mirande, Dijon, France.
Gresti
J
J
Bugaut
M
M
Gauthier
S
S
BezardJ
D000818:Animals; D002417:Cattle; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D005227:Fatty Acids / Q000032:analysis; D008892:Milk / Q000737:chemistry*; D014280:Triglycerides / Q000032:analysis*
D005227:Fatty Acids; D014280:Triglycerides