In vitro activity of the nitrogen fixation regulatory protein FIXJ from Rhizobium meliloti.
Cell extracts of an Escherichia coli strain that overproduces the regulatory protein FIXJ from Rhizobium meliloti promoted transcription of fixK, a known FIXJ-dependent gene, in a coupled transcription-translation assay. Activation by FIXJ was dependent on the sigma 70 holoenzyme form of RNA polymerase.
Batut
J
J
Department of Plant Pathology, University of California, Berkeley 94720.
Santero
E
E
Kustu
S
S
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation / Q000235:genetics*; D009841:Oligonucleotides / Q000737:chemistry; D011401:Promoter Regions, Genetic; D012231:Rhizobium / Q000235:genetics*; D012808:Sigma Factor / Q000235:genetics; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D009841:Oligonucleotides; D012808:Sigma Factor; C069289:FixJ protein, Bacteria