Fourier transform infrared spectroscopic studies of Ca(2+)-binding proteins.
The secondary structures of calmodulin and parvalbumin are well established from X-ray diffraction and nuclear magnetic resonance spectroscopic studies, which indicate that these proteins are predominantly alpha-helical in character. Recent infrared studies have nevertheless suggested that the helical structures present in these proteins in solution are not the standard alpha-helix but rather some kind of distorted helices [Trewhella, J., et al. (1989) Biochemistry 28, 1294]. The evidence for this was the unusually low amide I frequency for calmodulin and troponin C in 2H2O solution. The studies presented here, however, suggest that the helical structures in these proteins are not significantly distorted, for two reasons. First, distorted helical structures have weaker hydrogen bonds than the standard alpha-helix and would therefore be expected to absorb at a higher rather than a lower frequency. Second, distorted helical structures would absorb at an unusual frequency in H2O solutions which is not the case for the proteins studied here. The band frequency of these proteins is observed to occur at a frequency observed with other proteins known to contain predominantly alpha-helical structures. Quantitative analysis of the FT-IR spectra of calmodulin (67% alpha-helix) and parvalbumin (68% alpha-helix) in H2O in the presence of Ca2+ gives helical contents similar to those reported by X-ray studies. This raises the question as to why these proteins in H2O show a normal frequency for the presence of alpha-helical structures and an abnormal frequency in 2H2O. Addition of deuterated glycerol to the proteins in 2H2O solutions results in a significant shift of absorbance to higher frequency.(ABSTRACT TRUNCATED AT 250 WORDS)
Jackson
M
M
Steacie Institute for Molecular Sciences, National Research Council of Canada, Ottawa, Ontario.
Haris
P I
PI
Chapman
D
D
D002118:Calcium / Q000737:chemistry; D002135:Calcium-Binding Proteins / Q000737:chemistry*; D002147:Calmodulin / Q000737:chemistry; D002942:Circular Dichroism; D003903:Deuterium; D005583:Fourier Analysis; D005990:Glycerol; D006358:Hot Temperature; D010320:Parvalbumins / Q000737:chemistry; D011487:Protein Conformation; D013055:Spectrophotometry, Infrared; D014867:Water
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D010320:Parvalbumins; D014867:Water; D003903:Deuterium; D005990:Glycerol; D002118:Calcium