Application of the monodimensional electrophoresis for the separation of glycosaminoglycans on Cellogel cellulose acetate strip.
A monodimensional electrophoretic method for the separation of glycosaminoglycans on Titan III Zip Zone cellulose acetate plate based on their different electrophoretic mobilities in barium acetate and different solubilities in ethanol was applied to the Chemetron electrophoretic equipment. Improved timing of individual steps of electrophoretic run, additional cooling and pressure must be introduced for optimal separation of glycosaminoglycans mixture (dermatan sulphate, heparan sulphate, hyaluronic acid, chondroitin-4-sulphate, chondroitin-6-sulphate and keratan sulphate) resulting in five well separated sharp bands. By all these changes in the original procedure of Hopwood and Harrison, the separation of chondroitin-4-sulphate and chondroitin-6-sulphate was not achieved. The modified procedure on Cellogel strip is suitable for the screening of mucopolysaccharidoses.
Juretić
D
D
Institute of Medical Biochemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, Yugoslavia.
Bingulac-Popović
J
J
Lipovac
K
K
Ligutić
I
I
D004588:Electrophoresis, Cellulose Acetate / Q000295:instrumentation / Q000379:methods; D006025:Glycosaminoglycans / Q000302:isolation & purification*; D007202:Indicators and Reagents
D006025:Glycosaminoglycans; D007202:Indicators and Reagents