title: Estrogen target cells during the early stage of medullary bone osteogenesis: immunohistochemical detection of estrogen receptors in osteogenic cells of estrogen-treated male Japanese quail.
abstract: The localization of estrogen receptors (ER) in osteogenic cells during the early stage of medullary bone osteogenesis was studied immunohistochemically in the femurs of estrogen-treated male Japanese quail. Alkaline phosphatase (ALP) activity was used as a marker for osteogenic cells. ER immunostaining was observed in the nuclei of weak ALP-positive bone lining cells on the endosteal bone surface of nontreated birds. After 24 hours of estrogen treatment, nuclear immunostaining was detected in ALP-positive preosteoblasts on the endosteal bone surface. After 48 hours, the medullary bone appeared to some degree along the endosteal surface. ER immunostaining was observed in the nuclei of ALP-positive osteoblasts on the medullary bone surface. This study demonstrates that ER are present in osteogenic cells, and suggests that estrogen directly acts on medullary bone osteogenesis.
authors.affiliation: Graduate School of Science and Technology, Faculty of Agriculture, Niigata University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohashi
authors.forename: S
authors.initials: S
authors.lastname: Kusuhara
authors.forename: K
authors.initials: K
authors.lastname: Ishida
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003370:Coturnix / Q000378:metabolism*; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D005269:Femur / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D011960:Receptors, Estrogen / Q000032:analysis*
chemical_list: D011960:Receptors, Estrogen; D004958:Estradiol; D000469:Alkaline Phosphatase