Interferon-gamma binds to heparan sulfate by a cluster of amino acids located in the C-terminal part of the molecule.
Using three different approaches (domain mapping with monoclonal antibodies, limited enzymatic digestion and competition with synthetic peptides), we demonstrated that a cluster of basic amino acids on interferon-gamma is involved in its binding to heparan sulfate. This cluster (Lys125-Arg131) is localized in the C-terminal part of IFN-gamma. Once bound to heparin sulfate, IFN-gamma is protected against protease attack.
Lortat-Jacob
H
H
Laboratoire de Pathologie Cellulaire, CNRS URA 602, Institut Pasteur, Lyon, France.
Grimaud
J A
JA
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D001667:Binding, Competitive; D006497:Heparan Sulfate / Q000276:immunology / Q000378:metabolism*; D007371:Interferon-gamma / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D010449:Peptide Mapping
D000911:Antibodies, Monoclonal; D006497:Heparan Sulfate; D007371:Interferon-gamma