Separation of glutaraldehyde and some of its aldol condensation products by hydroxyl-aldehyde group affinity chromatography.
Glutaraldehyde exists in aqueous solution in equilibrium with monomers and polymers of cyclic and open-chain hemialdals and hydrates. At alkaline pH oligomeric and polymeric alpha,beta-unsaturated aldehyde derivatives are formed from primarily produced aldol condensation products. This communication reports a method for separation of such aldol condensates by means of a new high performance liquid chromatography technique based on the affinity of aldehyde groups for hydroxyl groups of a hydroxylated polyether matrix (Bio-Gel SEC-10). Five peaks corresponding to different aldol condensates of glutaraldehyde were obtained from the affinity column. They have been distinguished by 1H-NMR and UV spectroscopy. Kinetic measurements yielded formation rates for the different aldol condensates.
Holmquist
L
L
Kabi Pharmacia, Plasma Products, Stockholm, Sweden.
Lewin
M
M
D000438:Alcohols / Q000302:isolation & purification*; D000447:Aldehydes / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005976:Glutaral / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D011108:Polymers; D013056:Spectrophotometry, Ultraviolet
D000438:Alcohols; D000447:Aldehydes; D011108:Polymers; D005976:Glutaral