Cloning of the protein D2 gene of Pseudomonas aeruginosa and its functional expression in the imipenem-resistant host.
Protein D2 forms the water-filled pore across the outer membrane of Pseudomonas aeruginosa and allows the penetration of imipenem. We cloned the protein D2 gene by the antibody screening technique. When the imipenem-resistant mutant lacking protein D2 harbored the plasmid with the cloned D2 gene, the mutant overproduced protein D2 in the outer membrane. These transformants exhibited fully-restored imipenem susceptibility. The results prove unequivocally that protein D2 forms the imipenem-permeable pore in the P. aeruginosa outer membrane.
Yoneyama
H
H
Department of Cellular Information Sciences, Tokai University, School of Medicine, Isehara, Japan.
Nakae
T
T
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002463:Cell Membrane Permeability; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005798:Genes, Bacterial; D015378:Imipenem / Q000378:metabolism / Q000494:pharmacology*; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D012266:Ribose / Q000378:metabolism
D001426:Bacterial Proteins; D012266:Ribose; D015378:Imipenem