title: Jun-B gene expression mediated by the surface immunoglobulin receptor of primary B lymphocytes.
abstract: Stimulation of primary B lymphocytes induces the nuclear expression of TPA response element binding proteins that are recognized by anti-Jun antisera. To evaluate the profile of jun gene expression, RNA was extracted from B cells and probed for c-jun. Surprisingly, c-jun mRNA was not detected either before or after stimulation with anti-Ig. Instead, stimulation through the sIg antigen receptor, or with phorbol ester containing regimens, rapidly induced expression of the related jun-B. This demonstrates a lack of coordinate regulation for jun-B and c-jun expression in these primary B cells. The role of Jun-containing TRE binding proteins in promoting B cell cycle progression remains uncertain inasmuch as Jun-B has been associated with transcriptional inhibition of the TPA response element, rather than activation as produced by c-Jun.
authors.affiliation: Evans Memorial Department of Clinical Research, Boston University Medical Center, MA 02118.
authors.forename: J F
authors.initials: JF
authors.lastname: Tilzey
authors.forename: T C
authors.initials: TC
authors.lastname: Chiles
authors.forename: T L
authors.initials: TL
authors.lastname: Rothstein
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D005786:Gene Expression Regulation; D051379:Mice; D008807:Mice, Inbred BALB C; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics; D011971:Receptors, Immunologic / Q000502:physiology*; D013154:Spleen / Q000276:immunology; D014157:Transcription Factors / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D014157:Transcription Factors; D011505:Protein-Tyrosine Kinases