Antidyslipaemic action and role of CoA in lipid metabolism of mitochondria and peroxisomes.
Our study has evaluated the effect of the parenteral administration of CoA on the pattern of haematic lipids and on palmitate oxidation in liver mitochondria and peroxisomes of rats made hyperlipaemic with a high fat diet with or without CoA. Lipid fractions, total cholesterol, triacylglycerols, total lipids and nonesterified fatty acids (NEFA) were determined in blood. Palmitate oxidation was determined in liver peroxisomes and mitochondria incubated in modified Krebs-Henseleit solution with 100 microM 1-14C palmitate in the presence and absence of some cofactors. Our results show that in rats fed with a high fat diet there was an increase of all lipid fractions. The increase of all lipid components was lower in animals treated with CoA. In liver peroxisomes of rats fed with high fat diet an increase in palmitate oxidation, that is higher when CoA is parenterally administered, was observed. In addition, palmitate oxidation in mitochondria of rats treated with CoA reached values higher than those of control and of rats fed with a high fat diet without CoA.
Conte
A
A
Institute of Biochemistry, University of Pisa, Italy.
Palmieri
L
L
Segnini
D
D
Ronca
G
G
D000818:Animals; D002784:Cholesterol / Q000097:blood; D003065:Coenzyme A / Q000494:pharmacology* / Q000502:physiology; D004041:Dietary Fats / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008830:Microbodies / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D010168:Palmitates / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000097:blood
D004041:Dietary Fats; D005230:Fatty Acids, Nonesterified; D008055:Lipids; D010168:Palmitates; D014280:Triglycerides; D002784:Cholesterol; D003065:Coenzyme A