title: A technique for detecting antifungal activity of proteins separated by polyacrylamide gel electrophoresis.
abstract: A technique was developed for the detection of antifungal activity of proteins after discontinuous polyacrylamide gel electrophoresis under native conditions. The antifungal activity is detected as growth inhibition zones in a homogeneous fungal lawn, grown in an agar layer spread on top of the polyacrylamide gel. The position of proteins with antifungal activity can be determined on a diffusion blot prepared from the same gel. The technique is illustrated for three antifungal plant proteins, i.e. alpha-purothionin, Urtica dioica agglutinin, and tobacco chitinase.
authors.affiliation: F. A. Janssens Laboratory of Genetics, Catholic University of Leuven, Heverlee, Belgium.
authors.forename: M F
authors.initials: MF
authors.lastname: De Bolle
authors.forename: I J
authors.initials: IJ
authors.lastname: Goderis
authors.forename: F R
authors.initials: FR
authors.lastname: Terras
authors.forename: B P
authors.initials: BP
authors.lastname: Cammue
authors.forename: W F
authors.initials: WF
authors.lastname: Broekaert
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides; D002688:Chitinases / Q000737:chemistry / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D037102:Lectins / Q000737:chemistry / Q000494:pharmacology; D037121:Plant Lectins; D010940:Plant Proteins / Q000737:chemistry* / Q000494:pharmacology; D010947:Plants, Toxic; D014026:Nicotiana / Q000201:enzymology
chemical_list: D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; C016085:purothionin; D002688:Chitinases