Recombinant human stem cell factor, a c-kit ligand, stimulates hematopoiesis in primates.
Recombinant human stem cell factor (SCF) is homologous with recombinant rat SCF (rrSCF) and is a ligand for c-kit. We determined the influence of SCF on hematopoiesis in vitro and in vivo in baboons. In vitro, SCF alone stimulated little growth of hematopoietic colony-forming cells from baboon marrow, but did increase the number of colonies formed in response to erythropoietin (Epo), interleukin-3 (IL-3), and granulocyte-macrophage colony-stimulating factor (GM-CSF). In vivo, SCF caused an increase in the peripheral blood of the number of erythrocytes, neutrophils, lymphocytes, monocytes, eosinophils, and basophils. In marrow, it caused an increase in marrow cellularity and in the absolute number of colony-forming unit-granulocyte-monocyte (CFU-GM) and burst-forming unit-erythroid (BFU-E) in marrow following infusion of SCF. The in vivo stimulation of multiple lymphohematopoietic lineages corroborates previous in vitro studies and suggests a potentially important clinical role for SCF.
Andrews
R G
RG
Department of Pediatric, Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Knitter
G H
GH
Bartelmez
S H
SH
Langley
K E
KE
Farrar
D
D
Hendren
R W
RW
Appelbaum
F R
FR
Bernstein
I D
ID
Zsebo
K M
KM
D000818:Animals; D001854:Bone Marrow Cells; D006410:Hematopoiesis / Q000187:drug effects; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology*; D010215:Papio; D010641:Phenotype; D011994:Recombinant Proteins / Q000494:pharmacology
D016298:Hematopoietic Cell Growth Factors; D011994:Recombinant Proteins