title: [Immunoturbidimetric determination of C-3 components of complement systems using nonlinear calibration].
abstract: A method for the immunoturbidimetric analysis of the C-3-component in the complement system was elaborated by means of the antiserum Q-SwAHu/C3 USOL (SEVAG) Praha. A diluted human control serum USOL (SEVAG) Praha with declared values of plasma proteins was applied as a standard solution. The relation between concentration and absorption in an eight step calibration series is well described by a parabola of the 2nd degree. The precision in series and the accuracy of the method are mentioned. The proposed technique is in a relatively good correlation with the radial immunodiffusion according to MANCINI.
authors.affiliation: Abteilung der Klinischen Biochemie, CSFR.
authors.forename: J
authors.initials: J
authors.lastname: Rohácek
authors.forename: V
authors.initials: V
authors.lastname: Semrád
authors.forename: E
authors.initials: E
authors.lastname: Klierová
authors.forename: M
authors.initials: M
authors.lastname: Zápotocná
mesh_terms: D001798:Blood Proteins / Q000032:analysis; D003176:Complement C3 / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D005779:Immunodiffusion / Q000379:methods; D007223:Infant; D009391:Nephelometry and Turbidimetry / Q000379:methods; D012015:Reference Standards
chemical_list: D001798:Blood Proteins; D003176:Complement C3
vernacular_title: Immunoturbidimetrische Bestimmung der C-3-Komponente des Komplementsystems bei nichtlinearer Eichung.