title: Burkitt lymphoma cell lines are prone to recombination in the switch region of the Ig mu heavy chain locus.
abstract: Different recombinations have been found at the Ig heavy chain gene loci in a number of sublines of the Burkitt lymphoma (BL) cell line Namalwa, following prolonged in vitro culture. The Namalwa sublines examined are DNA fingerprint-identical and derived from a monoclonal source. Recombinant DNA clones were used to map the Ig heavy chain gene mutations to a region between the VDJ and C mu segment of the locus. This region is associated with Ig heavy chain class switching in normal B cells. Of 24 clones established from one subline, three were found to have additional VDJ-C mu region mutations, indicating a high frequency of mutation at this locus.
authors.affiliation: Leukaemia Research Fund Laboratories, Medical School, University of Newcastle upon Tyne, U.K.
authors.forename: P G
authors.initials: PG
authors.lastname: Middleton
authors.forename: I K
authors.initials: IK
authors.lastname: Williamson
authors.forename: J J
authors.initials: JJ
authors.lastname: Taylor
authors.forename: D
authors.initials: D
authors.lastname: Rowe
authors.forename: K
authors.initials: K
authors.lastname: Guy
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism; D001483:Base Sequence; D015139:Blotting, Southern; D002051:Burkitt Lymphoma / Q000235:genetics*; D003001:Cloning, Molecular; D015321:Gene Rearrangement; D006801:Humans; D007133:Immunoglobulin Joining Region / Q000235:genetics; D007075:Immunoglobulin M / Q000235:genetics; D007134:Immunoglobulin Switch Region / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007148:Immunoglobulin mu-Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic / Q000235:genetics*; D015183:Restriction Mapping; D014407:Tumor Cells, Cultured
chemical_list: D007133:Immunoglobulin Joining Region; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D007148:Immunoglobulin mu-Chains