A dihydrofolate reductase gene from Candida albicans: molecular cloning.
The dihydrofolate reductase gene from Candida albicans has been cloned and partially characterized. A genomic bank from C. albicans strain 10127/5 was constructed in Escherichia coli and screened for trimethoprim resistance. A plasmid pMF1, carrying the resistance marker was isolated and characterized by restriction mapping and Southern blotting. Cells harbouring pMF1 were as sensitive as the parental cells to a wide spectrum of antibacterial agents, except for trimethoprim; the dihydrofolate reductase activity from these cells was trimethoprim resistant.
Franceschi
M
M
Department of Animal Biology and Genetics, University of Firenze, Italy.
Denaro
M
M
Irdani
T
T
Lorenzetti
R
R
Mastromei
G
G
Skarmoutsou
E
E
Polsinelli
M
M
D002176:Candida albicans / Q000235:genetics*; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D010957:Plasmids; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D014295:Trimethoprim / Q000494:pharmacology
D014295:Trimethoprim; D013762:Tetrahydrofolate Dehydrogenase