Alpha 2,6 sialylation of N-acetyllactosaminic sequences in human colorectal cancer cell lines. Relationship with non-adherent growth.
In a previous work we found that human colorectal cancer tissues express increased levels of an alpha 2,6 sialyltransferase (alpha 2,6 ST) acting on N-acetyllactosaminic sequences (E.C. 2.4.99.1). In this study we have taken advantage of the known specificity of elderberry bark lectin (Sambucus nigra agglutinin, SNA) for NeuAc alpha 2, 6Gal/GalNAc structures to investigate the relationship between expression of alpha 2,6 sialyltransferase activity and occurrence of alpha 2,6-sialylated oligosaccharide sequences in human colorectal cancer cell lines. Three cell lines with opposite adhesion properties were used in this study: SW 948 cells grow adherent to the culture flask surface and express very low levels of enzyme activity; COLO 205 cells grow in non-adherent form and express the highest levels of alpha 2,6 ST activity; A non-adherent subline of SW 948 cells (SW 948 FL) was isolated and found to express high levels of alpha 2,6 ST activity. By using SNA-Sepharose chromatography we found that expression of alpha 2,6 ST activity correlates with the extent of alpha 2,6-sialylation of N-linked chains of glycoproteins but not with the presence of alpha 2,6-sialylated glycolipids.
Dall'Olio
F
F
Dipartimento di Patologia Sperimentale dell'Università di Bologna, Italy.
Malagolini
N
N
Di Stefano
G
G
Ciambella
M
M
Serafini-Cessi
F
F
D000606:Amino Sugars / Q000378:metabolism*; D002448:Cell Adhesion; D002455:Cell Division; D002846:Chromatography, Affinity; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000473:pathology; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005944:Glucosamine / Q000378:metabolism; D006801:Humans; D037102:Lectins; D008856:Microscopy, Fluorescence; D019158:N-Acetylneuraminic Acid; D037121:Plant Lectins; D054788:Ribosome Inactivating Proteins; D012794:Sialic Acids / Q000378:metabolism*; D012799:Sialyltransferases / Q000378:metabolism*; D013861:Thiocyanates; D014407:Tumor Cells, Cultured; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D000606:Amino Sugars; D005452:Fluoresceins; D005456:Fluorescent Dyes; D037102:Lectins; D037121:Plant Lectins; C050600:Sambucus nigra lectins; D012794:Sialic Acids; D013861:Thiocyanates; C000458:N-acetyllactosamine; D012799:Sialyltransferases; D054788:Ribosome Inactivating Proteins; D019158:N-Acetylneuraminic Acid; D016650:Fluorescein-5-isothiocyanate; D005944:Glucosamine; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase