Developmental analysis of the facets, a group of intronic mutations at the Notch locus of Drosophila melanogaster that affect postembryonic development.
The activity of the Notch locus of Drosophila melanogaster during embryogenesis is necessary for the correct segregation of neural from epidermal lineages. The action of Notch is not confined to embryogenesis but is also essential for normal development during the postembryonic stages. Its action is pleiotropic, as revealed by the existence of several classes of mutations which affect various imaginal structures. Here, we examine a group of six recessive mutations, the facets (fa, fa3, fag, fag-2, fafx and fasw), which affect eye and optic lobe morphology and have been previously shown to be associated with the insertion of transposable elements into an intronic region of Notch. Using both somatic recombination and gynandromorph analysis, we find that their behavior in a mosaic analysis is not identical. While in the majority of alleles abnormal Notch function in the retina is sufficient to induce optic lobe abnormalities, in the case of fag-2, a considerable number of individuals having mosaic retinas exhibit normal optic lobe structure. All the facet alleles appear to behave in a cell-autonomous manner. A developmental analysis of the eye and optic lobe defects associated with the facet mutations support the contention that Notch may be involved not only in the formation of certain structures but also in their maintenance.
Markopoulou
K
K
Department of Biology, Yale University, New Haven, Connecticut 06511.
Artavanis-Tsakonas
S
S
D000483:Alleles; D000818:Animals; D003433:Crosses, Genetic; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D005123:Eye / Q000196:embryology*; D005260:Female; D007438:Introns; D008297:Male; D008855:Microscopy, Electron, Scanning; D009030:Mosaicism; D009154:Mutation; D009420:Nervous System / Q000196:embryology; D009899:Optic Lobe, Nonmammalian / Q000196:embryology*; D011679:Pupa / Q000235:genetics / Q000254:growth & development; D012733:Sex Differentiation / Q000235:genetics