Cytokine production by the bladder carcinoma cell line 5637: rapid analysis of mRNA expression levels using a cDNA-PCR procedure.
We have studied cytokine expression by the human bladder carcinoma cell line 5637 using a cDNA-PCR procedure. Transcripts for interleukin-1 alpha (IL-1 alpha), IL-1 beta, IL-6, IL-7, IL-8, granulocyte-macrophage colony-stimulating factor (GM-CSF), G-CSF, M-CSF, tumor-necrosis factor-alpha (TNF-alpha), and TNF-beta were constitutively present, whereas IL-3, IL-4, IL-5, and IL-9 mRNA sequences could not be detected. This expression pattern was not altered after 12-O-tetradecanoyl-phorbol-13-acetate (TPA) stimulation (4 and 8 h) of 5637 cells. Relative expression levels of cytokines were assessed by limiting dilution of the cDNA pool. This procedure proved to be a semiquantitative technique when compared to Northern blot analysis.
Kaashoek
J G
JG
Department of Hematology, University Medical Center, Leiden, The Netherlands.
Mout
R
R
Falkenburg
J H
JH
Willemze
R
R
Fibbe
W E
WE
Landegent
J E
JE
D000199:Actins / Q000235:genetics; D015152:Blotting, Northern; D002460:Cell Line; D016207:Cytokines / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics*; D005091:Exons; D015870:Gene Expression; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics*; D001749:Urinary Bladder Neoplasms
D000199:Actins; D016207:Cytokines; D004273:DNA, Neoplasm; D012333:RNA, Messenger