Identification of an interferon-gamma-responsive element of a class II major histocompatibility gene in rat type 1 astrocytes.
Interferon-gamma(IFN-gamma) has been shown to induce class II major histocompatibility complex (MHC) antigens on several cell types. Previous analysis of cell lines including a glioblastoma multiforme line by our laboratory has mapped an IFN-gamma-responsive element to the upstream - 141 to - 109 base pair (bp) region of the DRA promoter. Using deletion mutants, this report shows that this same general region (-135 to -109 bp) is important for IFN-gamma induction in two other human glioma lines and more importantly in primary astrocytes. We have confirmed that this regulatory region of the HLA-DRA gene is necessary for IFN-gamma inducibility in astrocytes using a substitution mutant. Sequences beyond -135 bp do not appear to have any additional positive or negative elements.
Moses
H
H
Curriculum in Neurobiology, Immunology, University of North Carolina, Chapel Hill 27599.
Sasaki
A
A
Ting
J P
JP
D000818:Animals; D001253:Astrocytes / Q000276:immunology*; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D005786:Gene Expression Regulation / Q000187:drug effects; D005910:Glioma / Q000276:immunology; D006684:HLA-DR Antigens / Q000235:genetics*; D007371:Interferon-gamma / Q000494:pharmacology*; D009154:Mutation; D011401:Promoter Regions, Genetic; D051381:Rats; D011919:Rats, Inbred Strains; D014162:Transfection
D006684:HLA-DR Antigens; D007371:Interferon-gamma; D015500:Chloramphenicol O-Acetyltransferase