Time- and dose-dependent induction of invasive urinary bladder cancers by N-ethyl-N-(4-hydroxybutyl)nitrosamine in B6C3F1 mice.
A sequential investigation of N-ethyl-N-(4-hydroxybutyl)nitrosamine (EHBN) bladder carcinogenesis was performed in male B6C3F1 mice maintained ad libitum on tap water containing 0.025% EHBN for 4, 12, 20, 28 and 36 weeks. A total of 81 invasive tumors, comprising 55 squamous cell carcinomas (SCCs) (68%), 25 transitional cell carcinomas (TCCs) (31%) and 1 adenocarcinoma (1%) were found. Of these, 23 (22 SCCs and 1 TCC) demonstrated invasion to the prostate, 3 metastasized to the lung, and 2 spread by peritoneal seeding. The anaplastic grade and extent of invasion of the SCCs significantly exceeded those of the TCCs. The results suggested a histogenetic pathway from simple dysplasia through papillary or nodular dysplasia and/or carcinoma in situ to eventual development of invasive carcinomas.
Tamano
S
S
First Department of Pathology, Nagoya City University Medical School.
Hagiwara
A
A
Suzuki
E
E
Okada
M
M
Shirai
T
T
Fukushima
S
S
D000818:Animals; D002085:Butylhydroxybutylnitrosamine / Q000031:analogs & derivatives* / Q000633:toxicity; D002273:Carcinogens / Q000633:toxicity*; D002278:Carcinoma in Situ / Q000139:chemically induced / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000473:pathology; D002295:Carcinoma, Transitional Cell / Q000139:chemically induced / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009361:Neoplasm Invasiveness; D013997:Time Factors; D001743:Urinary Bladder / Q000187:drug effects / Q000473:pathology*; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced* / Q000473:pathology
D002273:Carcinogens; D002085:Butylhydroxybutylnitrosamine; C010723:N-ethyl-N-(4-hydroxybutyl)nitrosamine