title: Enzyme polymorphism of freshwater fish trypanosomes and its use for strain identification.
abstract: Sixteen strains of trypanosomes isolated from the blood of 10 freshwater fish species (all but one from South Bohemia) were characterized by enzyme analysis using disc electrophoresis in 5% polyacrylamide gel. Six enzymes were selected: ADH, LDH, MDH, ME, G6PDH and GPI. The most variable were ADH and LDH, whereas MDH, G6PDH and GPI showed the same pattern in all strains analysed. Cluster analysis indicated a very high degree of similarity among strains, even those isolated from phylogenetically distant hosts. Moreover, no correlation between enzyme pattern and host species was found.
authors.affiliation: Institute of Parasitology, Czechoslovak Academy of Sciences, Ceské Budĕjovice.
authors.forename: P
authors.initials: P
authors.lastname: Zajícek
mesh_terms: D000426:Alcohol Dehydrogenase / Q000032:analysis / Q000235:genetics; D000818:Animals; D004589:Electrophoresis, Disc; D004798:Enzymes / Q000032:analysis* / Q000235:genetics; D005393:Fish Diseases / Q000469:parasitology*; D005399:Fishes; D014644:Genetic Variation; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis / Q000235:genetics; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis / Q000235:genetics; D006651:Histocytochemistry; D007770:L-Lactate Dehydrogenase / Q000032:analysis / Q000235:genetics; D008291:Malate Dehydrogenase / Q000032:analysis / Q000235:genetics; D011110:Polymorphism, Genetic; D011528:Protozoan Infections / Q000469:parasitology; D011529:Protozoan Infections, Animal; D014351:Trypanosomatina / Q000145:classification* / Q000201:enzymology / Q000235:genetics
chemical_list: D004798:Enzymes; D000426:Alcohol Dehydrogenase; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005956:Glucose-6-Phosphate Isomerase