title: In vitro activity of the nitrogen fixation regulatory protein FIXJ from Rhizobium meliloti.
abstract: Cell extracts of an Escherichia coli strain that overproduces the regulatory protein FIXJ from Rhizobium meliloti promoted transcription of fixK, a known FIXJ-dependent gene, in a coupled transcription-translation assay. Activation by FIXJ was dependent on the sigma 70 holoenzyme form of RNA polymerase.
authors.affiliation: Department of Plant Pathology, University of California, Berkeley 94720.
authors.forename: J
authors.initials: J
authors.lastname: Batut
authors.forename: E
authors.initials: E
authors.lastname: Santero
authors.forename: S
authors.initials: S
authors.lastname: Kustu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation / Q000235:genetics*; D009841:Oligonucleotides / Q000737:chemistry; D011401:Promoter Regions, Genetic; D012231:Rhizobium / Q000235:genetics*; D012808:Sigma Factor / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D009841:Oligonucleotides; D012808:Sigma Factor; C069289:FixJ protein, Bacteria