title: Molecular cloning of protozoan myosin I heavy chain genes.
abstract: Myosins I are ubiquitous, nonfilamentous, actin-based mechanoenzymes originally discovered in protozoa. The extensive in vitro biochemical studies of purified protozoan myosins I are now being complemented with in vivo studies using cloned myosin I heavy chain genes and gene targeting techniques. Here we review briefly the systems and methods being used in these efforts to dissect protozoan myosin I structure and function using molecular genetic approaches.
authors.affiliation: Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
authors.forename: J A
authors.initials: JA
authors.lastname: Hammer
authors.forename: G
authors.initials: G
authors.lastname: Jung
mesh_terms: D000048:Acanthamoeba / Q000235:genetics; D000818:Animals; D003001:Cloning, Molecular; D004023:Dictyostelium / Q000235:genetics; D008722:Methods; D009218:Myosins / Q000235:genetics* / Q000378:metabolism
chemical_list: D009218:Myosins