title: Swine immunity to an attenuated Salmonella typhimurium mutant containing a recombinant plasmid which codes for production of a 31-kilodalton protein of Brucella abortus.
abstract: Salmonella typhimurium chi 4064, an attenuated delta cya delta crp mutant of S. typhimurium SR-11, was shown to be avirulent in swine. S. typhimurium chi 4064 was used as a carrier for plasmid pBA31-R7, which codes for the expression of a 31-kDa protein from Brucella abortus (BCSP31). Given orally, S. typhimurium chi 4064(pBA31-R7) colonized the intestine and mesenteric lymph nodes of 5- to 6-week-old crossbred swine. Orally immunized animals developed serum and intestinal antibody responses to the B. abortus 31-kDa protein and to salmonella endotoxin as measured by enzyme-linked immunosorbent assay. Similarly immunized swine did not develop delayed-type hypersensitivity following a subcutaneous injection of recombinant BCSP31. However, swine parenterally immunized with recombinant BCSP31 incorporated in Freund incomplete adjuvant did develop a delayed-type hypersensitivity response to the homologous antigen. The data indicated that oral presentation of antigen to swine in the context of recombinant S. typhimurium effective stimulated mucosal and systemic antibody-mediated immunity but failed to sensitize swine for either an antigen-specific delayed-type hypersensitivity or a blastogenic response to the cloned BCSP31.
authors.affiliation: Department of Veterinary Microbiology and Preventative Medicine, Iowa State University, Ames 50011.
authors.forename: T J
authors.initials: TJ
authors.lastname: Stabel
authors.forename: J E
authors.initials: JE
authors.lastname: Mayfield
authors.forename: L B
authors.initials: LB
authors.lastname: Tabatabai
authors.forename: M J
authors.initials: MJ
authors.lastname: Wannemuehler
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002003:Brucella abortus / Q000235:genetics*; D015169:Colony Count, Microbial; D007109:Immunity; D007421:Intestine, Small / Q000276:immunology / Q000382:microbiology; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D010957:Plasmids / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology*; D012463:Saliva / Q000276:immunology; D012481:Salmonella Infections, Animal / Q000276:immunology*; D012486:Salmonella typhimurium / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D013552:Swine; D014613:Vaccines, Attenuated / Q000008:administration & dosage
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D011994:Recombinant Proteins; D014613:Vaccines, Attenuated