title: Effects of temperature, amebic strain, and carbohydrates on Acanthamoeba adherence to corneal epithelium in vitro.
abstract: An in vitro coincubation assay was used to measure adhesion of radiolabeled Acanthamoeba trophozoites to corneal epithelium. Adhesion of amebae to corneal epithelium was higher at 25 degrees C than at 37 or 4 degrees C, did not consistently correlate with the reported pathogenicity of the strain of Acanthamoeba, and was inhibited by mannose and by methyl-alpha-D-mannopyranoside.
authors.affiliation: Department of Veterinary Pathobiology, University of Illinois, Urbana 61801.
authors.forename: L D
authors.initials: LD
authors.lastname: Morton
authors.forename: G L
authors.initials: GL
authors.lastname: McLaughlin
authors.forename: H E
authors.initials: HE
authors.lastname: Whiteley
mesh_terms: D000048:Acanthamoeba / Q000472:pathogenicity / Q000502:physiology*; D000268:Adhesiveness; D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology*; D002478:Cells, Cultured; D003315:Cornea / Q000382:microbiology*; D004848:Epithelium / Q000382:microbiology; D066298:In Vitro Techniques; D007634:Keratitis / Q000209:etiology; D008358:Mannose / Q000494:pharmacology; D011817:Rabbits; D013696:Temperature
chemical_list: D002241:Carbohydrates; D008358:Mannose