title: Penicillin-binding protein 1B of Escherichia coli exists in dimeric forms.
abstract: A high-molecular-weight band has been detected in Western immunoblots of nonboiled Escherichia coli samples incubated with polyclonal antiserum against penicillin-binding protein 1B (PBP 1B). This band was shown to be a dimer of PBP 1B. The dimer was more strongly associated with the envelope than the monomer, and it was still able to bind penicillin G. Analysis of the binding of fusion proteins of PBP 1B and beta-lactamase showed that the part of PBP 1B necessary for complex formation lies in the amino-terminal half of the protein.
authors.affiliation: Department of Molecular Cell Biology, University of Amsterdam, The Netherlands.
authors.forename: C A
authors.initials: CA
authors.lastname: Zijderveld
authors.forename: M E
authors.initials: ME
authors.lastname: Aarsman
authors.forename: T
authors.initials: T
authors.lastname: den Blaauwen
authors.forename: N
authors.initials: N
authors.lastname: Nanninga
mesh_terms: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D015153:Blotting, Western; D002352:Carrier Proteins; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000032:analysis* / Q000378:metabolism / Q000648:ultrastructure; D006602:Hexosyltransferases / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D046911:Macromolecular Substances; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D002267:Muramoylpentapeptide Carboxypeptidase; D010400:Penicillin G / Q000378:metabolism; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding; D011993:Recombinant Fusion Proteins; D012995:Solubility; D013329:Structure-Activity Relationship
chemical_list: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D002352:Carrier Proteins; D046911:Macromolecular Substances; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D011993:Recombinant Fusion Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D010400:Penicillin G