An unusual class I (Schiff base) fructose-1,6-bisphosphate aldolase from the halophilic archaebacterium Haloarcula vallismortis.
An electrophoretically homogeneous class I (Schiff base) alsolase has been isolated for the first time from the archaebacterial halophile Haloarcula (Halobacterium) vallismortis. The aldolase was characterized with respect to its molecular mass, amino acid composition, salt dependency, immunological cross-reactivity and kinetic properties. The subunit mass of aldolase is 27 kDa, which is much smaller than other class I aldolases. By the gel filtration method, the molecular mass of the halobacterial enzyme was estimated as 280 +/- 10 kDa, suggesting a decameric nature. In contrast to many halobacterial proteins, the H. vallismortis aldolase, though a halophilic enzyme, did not show an excess of acidic residues. Unlike the eukaryotic aldolases, the activity of the halobacterial enzyme was not affected by carboxypeptidase digestion. The general catalytic features of the enzyme were similar to its counterparts from other sources. No antigenic similarity could be detected between the H. vallismortis aldolase and class I aldolase from eubacteria and eukaryotes or class II halobacterial aldolases.
Krishnan
G
G
Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
Altekar
W
W
D000596:Amino Acids / Q000032:analysis; D001105:Archaea / Q000201:enzymology* / Q000276:immunology; D001894:Borohydrides / Q000494:pharmacology; D002268:Carboxypeptidases / Q000494:pharmacology; D043422:Carboxypeptidases A; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D004795:Enzyme Stability / Q000187:drug effects; D005634:Fructose-Bisphosphate Aldolase / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D006217:Halobacterium / Q000201:enzymology* / Q000276:immunology; D006868:Hydrolysis; D007700:Kinetics; D008970:Molecular Weight; D012492:Salts / Q000494:pharmacology; D012545:Schiff Bases; D013329:Structure-Activity Relationship
D000596:Amino Acids; D001894:Borohydrides; D012492:Salts; D012545:Schiff Bases; C025364:sodium borohydride; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; D005634:Fructose-Bisphosphate Aldolase