Comparison of a single-injection technique and inulin clearance for determining glomerular filtration rate in the sheep.
Glomerular filtration rate (GFR) was assessed over a 2 h period by the clearance of inulin (Cin) and in the subsequent 2 h period by a single-injection technique using radiolabelled sodium iothalamate (Ciot) or chromium-EDTA (CEDTA). Regression analysis yielded a significant correlation (P less than 0.05) between the standard clearance technique and the single-injection method, with the relationship Ciot = -10.67 + 1.25Cin with a significant correlation coefficient of r = 0.71 (P less than 0.02). There was better agreement between clearance values of inulin measured on successive days than between values determined by the single-injection technique on successive days. Cin remains the method of choice in sheep.
Wholohan
T
T
Department of Physiology and Pharmacology, University of Queensland, Australia.
Yesberg
N E
NE
Cross
R B
RB
D000818:Animals; D004492:Edetic Acid / Q000008:administration & dosage / Q000493:pharmacokinetics; D005069:Evaluation Studies as Topic; D005260:Female; D005919:Glomerular Filtration Rate; D007444:Inulin / Q000493:pharmacokinetics; D007483:Iothalamic Acid / Q000008:administration & dosage / Q000493:pharmacokinetics; D008657:Metabolic Clearance Rate; D012756:Sheep
D007483:Iothalamic Acid; D007444:Inulin; D004492:Edetic Acid