title: Time-course effects of ethanol pretreatment on hepatic necrosis and fat accumulation induced by aflatoxin B1 in the rat.
abstract: Effect of ethanol pretreatment on acute hepatotoxicity and hepatic fat accumulation induced by aflatoxin B1 (AFB1) was followed up to 120 h in male Wistar rats. Pretreatment with 4 oral doses of ethanol (4.0 g/kg body wt. each) at 48, 45, 24 and 21 h prior to AFB1 (2.0 mg/kg body wt.) single intraperitoneal administration caused a significant increase in the activity of plasma glutamic oxaloacetic transaminase (PGOT, 2.4-fold), plasma glutamic pyruvic transaminase (PGPT, 2.8-fold), liver triglycerides (2.3-fold) and the severity of liver necrosis at 72 h after AFB1 administration. The effect of ethanol pretreatment on an increase in the accumulation of liver cholesterol and cholesterol esters induced by AFB1 is additive in nature. In a time-course study, it was shown that liver necrosis and triglyceride, cholesterol and cholesterol ester accumulation occurred simultaneously in both groups of rats treated with AFB1 and ethanol-AFB1. These results suggest that fat accumulation per se is not a primary cause of liver necrosis induced by AFB1 and ethanol-AFB1.
authors.affiliation: Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thialand.
authors.forename: C
authors.initials: C
authors.lastname: Toskulkao
authors.forename: P
authors.initials: P
authors.lastname: Lohakachonpan
authors.forename: T
authors.initials: T
authors.lastname: Glinsukon
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000284:Administration, Oral; D016604:Aflatoxin B1; D000348:Aflatoxins / Q000633:toxicity*; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002273:Carcinogens / Q000633:toxicity*; D002784:Cholesterol / Q000378:metabolism; D004347:Drug Interactions; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism
chemical_list: D000348:Aflatoxins; D002273:Carcinogens; D014280:Triglycerides; D000431:Ethanol; D002784:Cholesterol; D016604:Aflatoxin B1; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase