Effects of vanadate in testicular capsule of the rat.
1. The effects of sodium orthovanadate (vanadate. 10(-5) to 3 x 10(-4) M) on testicular capsule of the rat, and the modifications of these effects by the calcium chelator EGTA (2mM), the calcium entry blockers verapamil (5 X 10(-5) M), nifedipine (10(-5 M) and diltiazem (5 x 10(-5) M), the (Na+ + K+)-ATPase inhibitor ouabain, the Na+/Ca2+ exchange inhibitor amiloride, and the calmodulin antagonists trifluoperazine (10(-4) M) and W-7 (5 x 10(-5) M) have been studied. 2. Vanadate induced contraction of the rat testicular capsule in a dose-dependent way (ED50: 82.8 +/- 7.4 x 10(-6) M). 3. The contraction induced by vanadate (3 and 30 x 10(-5) M) were abolished by EGTA and not modified by verapamil, nifedipine, flunarizine or diltiazem. 4. Amiloride (1 and 5 x 10(-5) M), but not ouabain (5 x 10(-5) and 10(-4) M), inhibit in a dose-dependent way the contraction induced by two doses of vanadate (3 and 30 x 10(-5) M). 5. Trifluoperazine and W-7 significantly inhibit the contraction of testicular capsule to 3 and 30 x 10(-5) M vanadate.
Sanchez
M
M
Laboratorio de Farmacologia (Departamento de Medicina), Facultad de Medicina, Universidad de Oviedo, Spain.
Andrés-Trelles
F
F
Hidalgo
A
A
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002147:Calmodulin / Q000037:antagonists & inhibitors; D004533:Egtazic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D014638:Vanadates / Q000494:pharmacology*
D002121:Calcium Channel Blockers; D002147:Calmodulin; D014638:Vanadates; D004533:Egtazic Acid; D010042:Ouabain; D000584:Amiloride; D002118:Calcium