Purification of merozoites of Theileria sergenti from infected bovine erythrocytes.
A simple and rapid method for purifying merozoites of Theileria sergenti from infected bovine erythrocytes was developed. Infected erythrocytes were lysed by the use of a cytolytic toxin produced by Aeromonas hydrophila and the lysate was subjected to ultracentrifugation in a Percoll discontinuous density gradient. Pure and morphologically intact merozoites free of erythrocyte membrane were recovered from a band formed at the interface of 40% and 60% (by vol.) Percoll solutions. In this merozoite fraction, contamination of erythrocyte membrane proteins was not detected as examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Sugimoto
C
C
National Institute of Animal Health, Ibaraki, Japan.
Sato
M
M
Kawazu
S
S
Kamio
T
T
Fujisaki
K
K
D000333:Aeromonas; D000818:Animals; D016782:Apicomplexa / Q000302:isolation & purification* / Q000648:ultrastructure; D002417:Cattle; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000469:parasitology*; D006460:Hemolysin Proteins / Q000378:metabolism; D006461:Hemolysis; D008854:Microscopy, Electron; D015800:Protozoan Proteins / Q000032:analysis; D013801:Theileriasis / Q000469:parasitology
D006460:Hemolysin Proteins; D015800:Protozoan Proteins