Opioidergic control of gonadotrophin secretion in the prepubertal gilt during restricted feeding and realimentation.
Prepubertal gilts, having undergone a 7-day period of feed restriction to a maintenance ration, were allocated to one of 4 treatments; restricted feeding at 09:00 and 17:00 h for an 8th day both with (Group RN) and without (Group R) administration of the opioid antagonist naloxone hydrochloride (1 mg.kg-1 at 09:30 h followed by 0.5 mg.kg-1 at hourly intervals for 7 h), or feed to appetite with (Group ALN) and without (Group AL) naloxone administration. Gilts were bled at 10-min intervals on Day 8 from morning to evening feed and plasma LH, FSH and prolactin concentrations were measured by radioimmunoassay. Compared with Group R gilts, Group AL gilts exhibited significantly (P less than or equal to 0.05) higher mean and maximum LH concentrations and pulsatility, lower prolactin concentrations (P less than 0.05) but no significant difference in FSH secretion. Naloxone significantly depressed the increase in LH after re-feeding (Group ALN) (P less than 0.05). Once again there were no significant effects on FSH secretion. Naloxone also significantly depressed prolactin secretion in feed-restricted gilts (P less than 0.05). These results confirm that re-feeding of feed-restricted prepubertal gilts stimulates an immediate increase in LH secretion and that this elevation is not mediated via a suppression of inhibitory endogenous opioidergic tone. Rather, naloxone treatment appeared to expose a latent inhibition of LH secretion. The control of LH secretion is distinct from that of FSH in this model.
Cosgrove
J R
JR
AFRC Research Group on Hormones and Farm Animal Reproduction, University of Nottingham Faculty of Agricultural and Food Science, Loughborough, Leics, UK.
Booth
P J
PJ
Foxcroft
G R
GR
D000821:Animal Feed; D000818:Animals; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D005508:Food Deprivation / Q000502:physiology*; D006065:Gonadotropins, Pituitary / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000097:blood; D009270:Naloxone / Q000494:pharmacology*; D011388:Prolactin / Q000097:blood; D012741:Sexual Maturation / Q000502:physiology*; D013552:Swine / Q000097:blood / Q000502:physiology*
D006065:Gonadotropins, Pituitary; D009270:Naloxone; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone