Biochemical characterization and tissue distribution of hamster complement C1s.
Several mAb (PG11, NG7, and ED4) against hamster complement C1s were obtained. PG11 and NG7 were shown to cross-react with human and rat C1s. By using an immunohistochemical method, we examined localization of C1s in tissues of hamsters and rats. Present results revealed a widespread yet specific staining of hamster C1s which is associated with endoderm-, mesoderm-, and neuroectoderm-derived cells. For example, chondrocyte of hyaline cartilage and surface epithelium of the stomach were strongly positive. Intestinal epithelium, muscle cells, pia mater and epithelium of the choroid plexus of the ventricle, and hepatocytes were also stained. The synthesis of hamster C1s in these organs was confirmed by RNA blot hybridization. Secretion of C1s into the culture medium was revealed by immunoblot analysis in cell lines of hepatocytes, kidney cells, and myoblasts of rat or hamster.
Sakiyama
H
H
Division of Physiology and Pathology, National Institute of Radiological Sciences, Chiba-shi, Japan.
Yamaguchi
K
K
Chiba
K
K
Nagata
K
K
Taniyama
C
C
Matsumoto
M
M
Suzuki
G
G
Tanaka
T
T
Tomoasawa
T
T
Yasukawa
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D002478:Cells, Cultured; D003173:Complement C1s / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D006224:Cricetinae; D015870:Gene Expression; D006026:Glycoside Hydrolases / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008970:Molecular Weight; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; D006026:Glycoside Hydrolases; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D003173:Complement C1s