Glycosphingolipid specificity of the human sulfatide activator protein.
The interaction of the sulfatide activator protein with different glycosphingolipids have been studied in detail. The following findings were made. 1. The sulfatide activator protein forms water-soluble complexes with sulfatides [Fischer, G. and Jatzkewitz, H. (1977) Hoppe-Seyler's Z. Physiol. Chem. 356, 6588-6591] and various other glycospingolipids. 2. In the absence of degrading enzymes the activator protein acts in vitro as a glycosphingolipid transfer protein, transporting glycosphingolipids from donor to acceptor liposomes. Lipids having less than three hexoses, e.g. galactosylceramide, sulfatide and ganglioside GM3 were transferred at very slow rates, whereas complex lipids such as gangliosides GM2, GM1 and GD1a were transferred much faster than the former. The transfer rate increased with increasing length of the carbohydrate chain of the lipid molecules. 3. Both the acyl residue in the ceramide moiety and the nature of the carbohydrate chain are significant for recognition of the glycosphingolipids by the sulfatide activator protein. Apparently, both residues serve as an anchor and the longer they are the better they are recognized by the protein. 4. In the absence of activator protein, degradation rates of sulfatide derivatives by arylsulfatase A, and of ganglioside GM1 derivatives by beta-galactosidase, increase with decreasing length of acyl residues in their hydrophobic ceramide moiety. Addition of activator protein stimulates the degradation of only those GM1 and sulfatide derivatives that have long-chain fatty acids in their hydrophobic ceramide anchor.
Vogel
A
A
Intitut für Organische Chemie und Biochemie, Universität Bonn, Federal Republic of Germany.
Schwarzmann
G
G
Sandhoff
K
K
D002518:Ceramides / Q000378:metabolism; D005679:G(M3) Ganglioside / Q000378:metabolism; D005699:Galactosylceramides / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006028:Glycosphingolipids / Q000378:metabolism*; D006801:Humans; D008081:Liposomes; D049231:Saposins; D049128:Sphingolipid Activator Proteins; D014461:Ultracentrifugation
D002518:Ceramides; D005679:G(M3) Ganglioside; D005699:Galactosylceramides; D006023:Glycoproteins; D006028:Glycosphingolipids; D008081:Liposomes; C483345:PSAP protein, human; D049231:Saposins; D049128:Sphingolipid Activator Proteins