title: [Comparative studies of the filtration behavior of bacteria and organic particles in porous groundwater conductors. Fundamentals and methods].
abstract: In subsurface aquatic environments two groups of micro-organisms are observed: allochthonous bacteria and viruses, which as contaminants are eliminated from water after some time, and autochthonous groundwater micro-organisms, which belong to the natural subsurface environment and may reach very high abundances under favourable conditions--especially in the presence of a high nutrient supply. The migration of micro-organisms is controlled by flow length dependent transport processes (advection--dispersion, adsorption--desorption), and predominantly by filtration. This can be described on the basis of an expanded advection--dispersion concept. The filter effects in a certain porous aquifer can be quantified by the filter efficiency (filter factor) as a measure of the specific decrease of an initial concentration on a certain flow length. Recent laboratory experiments show that for sand the filter factor depends on the respective microbial species and is highly correlated to the effective grain diameter of the porous material, which is routinely determined in hydrogeology. Experiments with columns filled with quartz sand using the bacteria species Escherichia coli ATCC 11229, Pseudomonas cepacia DSM 50181, Streptococcus faecalis ATCC 6569, and polystyrene beads with similar density and diameters show that the filter factor is controlled by the grain size of filter material, the flow velocity, the diameter of the particle and the ionic strength of the water: The filter factor is specific for each microbial species for the same conditions of the aquatic environment. The filter factor decreases one order of magnitude if the flow velocity increases in the same order. A major control of the filter factor is the grain size. Conventionally the grain size is used as characteristic length instead of the pore size which, although it should be the real reference date, is relatively difficult to measure. For the assessment of the filter factor, the grain size d10, taken from the grain size distribution curve, can be used. The influence of the particle diameter on the filter factor, which was predicted by the filtration theory, was confirmed. The minimum values of the filter factor were encountered at particle diameters of about 1 micron, which is about the size of bacteria. The filter factor is influenced strongly by the ionic strength in water with low ionic strength, whereas in water of higher ionic strength its influence can be neglected. These relationships can be formulated into empirical equations, which allow prediction of the filter factor for given hydraulic conditions.
authors.affiliation: Geologisch-Paläontologisches Institut und Museum der Christian-Albrechts-Universität Kiel.
authors.forename: G
authors.initials: G
authors.lastname: Matthess
authors.forename: E
authors.initials: E
authors.lastname: Bedbur
authors.forename: K O
authors.initials: KO
authors.lastname: Gundermann
authors.forename: M
authors.initials: M
authors.lastname: Loof
authors.forename: D
authors.initials: D
authors.lastname: Peters
mesh_terms: D018407:Bacterial Physiological Phenomena; D013293:Enterococcus faecalis / Q000502:physiology; D004926:Escherichia coli / Q000502:physiology; D005374:Filtration; D005618:Fresh Water; D008863:Microspheres; D011549:Pseudomonas / Q000502:physiology; D012987:Soil; D014871:Water Microbiology
chemical_list: D012987:Soil
vernacular_title: Vergleichende Untersuchung zum Filtrationsverhalten von Bakterien und organischen Partikeln in Porengrundwasserleitern. I. Grundlagen und Methoden.