title: Changes in temporal and spatial patterns of Gi protein expression in postimplantation mouse embryos.
abstract: We previously demonstrated the presence of GTP-binding proteins, G proteins, in the preimplantation mouse embryo (Jones and Schultz, 1990. Dev. Biol. 139, 250-262). These studies have been extended to the Day 6.5, 7.5, and 8.5 gestation embryo by employing PT-catalyzed ADP-ribosylation and immunoblotting techniques. We report here that the amount of embryonic alpha i increases from Day 6.5 to Day 7.5 of gestation, and remains at about the same level at Day 8.5. In contrast, the extent of PT-catalyzed ADP-ribosylation of Gi alpha protein(s) decreases between Days 6.5 and 7.5--this decrease is global and not restricted to a particular germ layer of the Day 7.5 embryo--and then dramatically increases by Day 8.5 of gestation. In the Day 8.5 gestation embryo, the extent of PT-catalyzed ADP-ribosylation of Gi alpha proteins increases along the anterior-posterior axis, whereas the amount of immunoreactive alpha i subunit decreases along this axis. By using a combination of PT-catalyzed ADP-ribosylation and immunoprecipitation with antisera specific for alpha i1, alpha i2, or alpha i3, we report that all three alpha i subtypes are present in the Day 8.5 gestation mouse embryo. Results of these experiments suggest that an activation of Gi proteins occurs between Days 6.5 and 7.5 of gestation in the postimplantation embryo, a time during which the embryo is gastrulating, and that a decreasing gradient of activation exists along the anterior to posterior axis in the Day 8.5 gestation embryo. Last, we report that oocytes, eggs, and preimplantation embryos possess all three subtypes of alpha i.
authors.affiliation: Department of Biology, University of Pennsylvania, Philadelphia 19104-6018.
authors.forename: J
authors.initials: J
authors.lastname: Jones
authors.forename: C Y
authors.initials: CY
authors.lastname: Logan
authors.forename: R M
authors.initials: RM
authors.lastname: Schultz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001755:Blastocyst / Q000502:physiology; D004622:Embryo, Mammalian / Q000502:physiology*; D005314:Embryonic and Fetal Development; D005260:Female; D019204:GTP-Binding Proteins / Q000032:analysis / Q000096:biosynthesis*; D007106:Immune Sera; D015151:Immunoblotting; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009028:Morula / Q000502:physiology; D009243:NAD / Q000378:metabolism; D009865:Oocytes / Q000502:physiology; D037342:Pertussis Toxin; D011247:Pregnancy; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D007106:Immune Sera; D046911:Macromolecular Substances; D010566:Virulence Factors, Bordetella; D009243:NAD; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins