title: Queuine metabolism and cadmium toxicity in Drosophila melanogaster.
abstract: Queuine can replace guanine in the anticodon of certain tRNAs and is a hypermodified guanine derivative that can be synthesized by bacteria but not by mice. The study demonstrates that Drosophila can incorporate dietary queuine into tRNA but cannot synthesize it de novo for this purpose. Since an earlier study had shown that dietary CdCl2 caused Drosophila to increase greatly the proportion of queuine-containing tRNA over non-queuine tRNA the ability of dietary queuine to counteract cadmium toxicity was evaluated. When queuine was present in the cadmium-containing medium more pupae matured into adults than when queuine was absent. Other studies had demonstrated that the transglycosylase enzyme, that catalyzes the replacement of guanine in the anticodon of tRNA by queuine, is present in Drosophila larvae but the tRNA is virtually devoid of queuine. This study shows that in the presence of dietary queuine the larval tRNA contains abundant amounts of queuine. Therefore, we postulate a significant role for bacteria in supplying queuine to Drosophila for its incorporation into tRNA and that the control of this process by Drosophila is passive, i.e. is not an essential feature in differentiation.
authors.affiliation: University of Tennessee, College of Veterinary Medicine, Knoxville.
authors.forename: T J
authors.initials: TJ
authors.lastname: Siard
authors.forename: K B
authors.initials: KB
authors.lastname: Jacobson
authors.forename: W R
authors.initials: WR
authors.lastname: Farkas
mesh_terms: D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000378:metabolism*; D006147:Guanine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism; D012343:RNA, Transfer / Q000096:biosynthesis
chemical_list: D002104:Cadmium; D006147:Guanine; C019549:queuine; D012343:RNA, Transfer