Expression of a chimaeric granule-bound starch synthase-GUS gene in transgenic potato plants.
Granule-bound starch synthase is the key enzyme in amylose synthesis. The regulation of this gene was investigated using a chimaeric gene consisting of a 0.8 kb 5' upstream sequence of the granule-bound starch synthase gene from potato and the beta-glucuronidase gene which was introduced into potato using an Agrobacterium tumefaciens binary vector system. The chimaeric gene was highly expressed in stolons and tubers, whereas the expression in leaves, stems or roots from greenhouse-grown plants was relatively low. However, leaves from in vitro grown plantlets exhibited an elevated GUS expression. The expression of the chimaeric gene was inducible in leaves by growth on relatively high concentrations of sucrose, fructose and glucose and was about 30- to 50-fold higher than in leaves from greenhouse-grown plants. The granule-bound starch synthase gene is expressed organ-specifically since stolons and tubers showed GUS activities 125- to 3350-fold higher than in leaves. The activities in these two organs are 3- to 25-fold higher than the expression of the CaMV-GUS gene. Histochemical analysis of different tissues showed that only certain regions of leaves and roots express high GUS activities. Stolons and tubers show high expression.
Visser
R G
RG
Department of Plant Breeding, Wageningen Agricultural University, Netherlands.
Stolte
A
A
Jacobsen
E
E
D016960:Agrobacterium tumefaciens / Q000235:genetics; D015139:Blotting, Southern; D002241:Carbohydrates / Q000494:pharmacology; D002678:Chimera / Q000235:genetics; D046508:Culture Techniques; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D005966:Glucuronidase / Q000235:genetics*; D010944:Plants / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000378:metabolism; D011198:Solanum tuberosum / Q000201:enzymology / Q000235:genetics*; D013214:Starch Synthase / Q000235:genetics*; D014162:Transfection
D002241:Carbohydrates; D011993:Recombinant Fusion Proteins; D013214:Starch Synthase; D005966:Glucuronidase