Use of monoclonal antibodies to recognise osteoclasts in routinely processed bone biopsy specimens.
In decalcified (5% nitric acid) and undecalcified (glycol-methacrylate or resin embedded) routinely processed bone specimens osteoclasts against resorbing surfaces were identified with monoclonal antibodies directed against leucocyte common antigen (LCA) (PD7/26, 2B11), CD68 (KP1), and gpIIIa (Y2/51) but not against HLA-DR (CR3/43 and Ta11B5). Mononuclear cells on resorbing surfaces and occasional mononuclear cells against or near resting surfaces showed a similar pattern of reactivity. This study shows that immunohistochemistry is a sensitive and useful technique for identifying osteoclasts in routinely processed bone specimens. It also suggests a role for mononuclear cells (possibly pre-osteoclasts) in bone resorption.
Athanasou
N A
NA
Nuffield Department of Pathology, University of Oxford, John Radcliffe Hospital, Headington.
Puddle
B
B
Quinn
J
J
Woods
C G
CG
D000911:Antibodies, Monoclonal; D001706:Biopsy; D001847:Bone Diseases / Q000473:pathology; D001842:Bone and Bones / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D010003:Osteoarthritis / Q000473:pathology; D010010:Osteoclasts / Q000473:pathology*
D000911:Antibodies, Monoclonal