title: [Listeria monocytogenes contamination of raw milk from different regions of Anatolia and pasteurized milk sold in Ankara].
abstract: In this study 77 raw milk samples from different regions of Anatolia and 22 pasteurized milk samples sold in Ankara were investigated for isolation of L. monocytogenes. For the isolation of Listeria, each sample was plated directly onto McBride's Listeria Agar (MLA) and on the other hand four processing methods (2 long cold enrichment and 2 shortened warm enrichment procedures, followed by plating) were used. Listeria colonies on MLA medium were examined by the Henry Method of oblique lighting. Suspected colonies from MLA were subjected to biochemical tests to confirm identity. 14 samples (18.2%) of all examined samples raw milk were determined to be positive for Listeria monocytogenes. We found that Tryptose Phosphate Broth enrichment procedure gave significantly better results than others and allows L. monocytogenes isolation from milk most frequently. All of tested pasteurized milk samples were negative for L. monocytogenes.
authors.affiliation: Ankara Universitesi, Ziraat Fakültesi, Gida Bilimi ve Teknolojisi Bölümü, Biyoteknoloji Anabilim Dali.
authors.forename: A
authors.initials: A
authors.lastname: Sharif
authors.forename: N
authors.initials: N
authors.lastname: Tunail
mesh_terms: D000818:Animals; D003470:Culture Media; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D008892:Milk / Q000382:microbiology*; D014421:Turkey
chemical_list: D003470:Culture Media
vernacular_title: Ceşitli yörelere ait çiğ sütler ile Ankara piyasasinda satilan pastörize sütlerde Listeria monocytogenes kontaminasyonunun araştirilmasi.