Elastolytic activity of human monocytes from synovial fluid and blood of patients with arthritis. Relations to levels of interleukin 6 and soluble interleukin 2 receptor.
Synovial fluid (SF) and blood from 24 patients with non-traumatic, sterile hydarthron were examined for monocyte elastolysis (MøE) and for levels of interleukin 6 (IL-6) and of soluble interleukin 2 receptor (sIL-2R). Six patients had osteoarthrosis (OA) and 18 patients had inflammatory hydarthron (IH), 10 of whom had rheumatoid arthritis (RA). Blood MøE was lower in OA than in IH, both measured as basal MøE activity and after in vitro stimulation with immune complexes and phorbol myristate acetate (PMA). SF MøE was higher than MøE in blood (p less than 0.01). This increase in SF MøE could be mimicked in vitro by prestimulation of blood Mø with low levels of IC. SF IL-6 and sIL-2R were also elevated (p less than 0.01). All three parameters correlated to the degree of joint inflammation evaluated by SF leucocyte level, complement activation, blood C Reactive Protein, and to the clinical evaluation of the joint. The increase in SF MøE, IL-6 and sIL-2R in patients with IH, points to a stimulation of Mø and lymphocytes in the joint.
Jensen
H S
HS
Department of rheumatology 232, Hvidovre University Hospital, Denmark.
Tvede
N
N
Diamant
M
M
Mogensen
H H
HH
Hansen
M B
MB
Thomsen
B S
BS
Pedersen
P B
PB
Bendtzen
K
K
D000328:Adult; D000368:Aged; D001168:Arthritis / Q000097:blood / Q000378:metabolism*; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000378:metabolism; D004547:Elastic Tissue / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000097:blood / Q000502:physiology; D015850:Interleukin-6 / Q000097:blood* / Q000502:physiology; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism / Q000502:physiology*; D010003:Osteoarthritis / Q000097:blood / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000097:blood* / Q000502:physiology; D013582:Synovial Fluid / Q000737:chemistry / Q000166:cytology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism / Q000502:physiology
D015850:Interleukin-6; D015375:Receptors, Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma