Immunization by gamma-IFN-treated B16-F10.9 melanoma cells protects against metastatic spread of the parental tumor.
B16-F10.9 is a highly metastatic clone of the B16-F10 melanoma line, that expresses low levels of MHC class-I antigens. F10.9 cells transfected with H-2Kb are highly immunogenic and consequently exhibit a low metastatic phenotype. Treatment with gamma-IFN elevated H-2Kb and H-2Db cell surface expression of F10.9 cells to levels much higher than did transfection of these genes. Yet, following intravenous injection, the gamma-IFN treated cells generated high loads of lung metastases. However, when tested for their immunogenic effect, they elicited CTL and were sensitive to CTL. Immunization with both the positive transfectant KI and the gamma-IFN-treated F10.9 cells protected in vivo against metastatic spread of a subsequent transplant of parental F10.9 cells. The protection elicited by KI transfectants was more effective than the protection by gamma-IFN-treated cells.
Porgador
A
A
Department of Cell Biology, Weizmann Institute of Science, Rehovot, Isreal.
Brenner
B
B
Vadai
E
E
Feldman
M
M
Eisenbach
L
L
D000818:Animals; D000911:Antibodies, Monoclonal; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000032:analysis* / Q000235:genetics; D007114:Immunization; D007371:Interferon-gamma / Q000627:therapeutic use*; D008546:Melanoma, Experimental / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis; D011994:Recombinant Proteins; D013154:Spleen / Q000276:immunology
D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; D011994:Recombinant Proteins; D007371:Interferon-gamma