title: Interpretive criteria and quality control guidelines for Neisseria gonorrhoeae susceptibility test standardization for cefotetan.
abstract: Cefotetan was tested in a multilaboratory study to standardize susceptibility testing criteria and quality control guidelines for Neisseria gonorrhoeae. Cefotetan was most active against penicillinase-producing and penicillin-susceptible strains (MIC for 50% of strains tested, 0.5 micrograms/ml) and was least active against the chromosomally resistant isolates (MIC for 50% of strains tested, 2 micrograms/ml). The recommended 30-micrograms disk cefotetan interpretive criteria were as follows: susceptible at greater than or equal to 26 mm (less than or equal to 2 micrograms/ml), intermediate at 20 to 25 mm (4 micrograms/ml), and resistant at less than or equal to 19 mm (greater than or equal to 8 micrograms/ml). Quality control guidelines for agar dilution and disk diffusion tests were established by using numerous GC agar lots, three cefotetan 30-micrograms disk lots, two quality control organisms, and a volume of tests consistent with National Committee for Clinical Laboratory Standards M23-T guidelines.
authors.affiliation: University of Iowa College of Medicine, Iowa City.
authors.forename: R N
authors.initials: RN
authors.lastname: Jones
authors.forename: E H
authors.initials: EH
authors.lastname: Gerlach
authors.forename: F P
authors.initials: FP
authors.lastname: Koontz
authors.forename: P R
authors.initials: PR
authors.lastname: Murray
authors.forename: M A
authors.initials: MA
authors.lastname: Pfaller
authors.forename: J A
authors.initials: JA
authors.lastname: Washington
authors.forename: M E
authors.initials: ME
authors.lastname: Erwin
authors.forename: C C
authors.initials: CC
authors.lastname: Knapp
mesh_terms: D015313:Cefotetan / Q000494:pharmacology*; D005069:Evaluation Studies as Topic; D006069:Gonorrhea / Q000188:drug therapy / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards*; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D010403:Penicillin Resistance / Q000235:genetics; D010405:Penicillinase / Q000096:biosynthesis; D011786:Quality Control
chemical_list: D015313:Cefotetan; D010405:Penicillinase