In vitro assembly of the functional porin trimer from dissociated monomers in Pseudomonas aeruginosa.
The molecular weights of monomeric and oligomeric forms of the newly identified porins, protein D2, of the outer membrane of Pseudomonas aeruginosa appeared to be 47,000 and 137,000, respectively, as determined by the light scattering technique. Presence of the trimeric aggregates of the homologous subunits in the intact outer membrane, the liposome membrane, and the non-ionic surfactant were confirmed through cross-linking experiments and immunoblotting techniques. The protein D2 monomers prepared in 0.1% of sodium dodecyl sulfate at 23 degrees C spontaneously reassembled into the trimeric aggregate when the surfactant dropped below critical concentration. The diffusion rates of saccharides and beta-lactam antibiotics through the liposome membranes reconstituted from the reassembled protein D2 trimers were indistinguishable from those of the native protein D2. This study shed some light on the porin trimer assembly as well as on the mechanism of carbapenem diffusion through the protein D2 pores.
Yoshihara
E
E
Department of Cellular Information Sciences, Tokai University School of Medicine, Isehara, Japan.
Yoneyama
H
H
Nakae
T
T
D000900:Anti-Bacterial Agents / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D015153:Blotting, Western; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D007769:Lactams; D008970:Molecular Weight; D009005:Monosaccharides / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D003432:Cross-Linking Reagents; D007769:Lactams; D009005:Monosaccharides