Fibrinogen does not protect von Willebrand factor against proteolysis by human cathepsin G.
The susceptibility of von Willebrand factor /vWF/ to digestion by human neutrophil cathepsin G in highly purified FVIII/vWF concentrate /BHS/ and in cryoprecipitate has been studied. In contrast to human neutrophil elastase, cathepsin G inactivates and degrades vWF not only in BHS but also in cryoprecipitate. Fibrinogen added in excess to purified FVIII/vWF concentrate does not protect vWF against cathepsin G proteolysis. Biological significance of this phenomena are discussed.
Jeleńska
M
M
Institute of Hematology, Warsaw, Poland.
Kopeć
M
M
Sopata
I
I
Vigh
Z
Z
Scharrer
I
I
Breddin
K
K
D056649:Cathepsin G; D002403:Cathepsins / Q000494:pharmacology* / Q000502:physiology; D005169:Factor VIII / Q000187:drug effects / Q000378:metabolism; D005340:Fibrinogen / Q000494:pharmacology*; D006801:Humans; D011487:Protein Conformation / Q000187:drug effects; D012697:Serine Endopeptidases; D014841:von Willebrand Factor / Q000187:drug effects* / Q000378:metabolism
D014841:von Willebrand Factor; D005169:Factor VIII; D005340:Fibrinogen; D002403:Cathepsins; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G