title: UVA-induced lipid peroxidation in cultured human fibroblasts.
abstract: The UVA irradiation of cultured human fibroblasts leads to the formation and to the release of thiobarbituric acid-reactive substances in the supernatant. The major thiobarbituric acid-reactive substance is identified by fluorescence spectroscopy and HPLC, as malondialdehyde or malondialdehyde-forming substances under the thiobarbituric acid assay conditions. Malondialdehyde formation strongly suggests a UVA-induced lipid peroxidation. Lipid peroxidation is also supported by the inhibitory effect of D,L-alpha-tocopherol, the well-known chain breaking antioxidant, by the additional malondialdehyde formation in the dark after the photooxidative stress and by membrane damage revealed by lactate dehydrogenase leakage.
authors.affiliation: Laboratoire de Dermatologie, INSERM U. 312, Hôpital Henri Mondor, Créteil, France.
authors.forename: P
authors.initials: P
authors.lastname: Morlière
authors.forename: A
authors.initials: A
authors.lastname: Moysan
authors.forename: R
authors.initials: R
authors.lastname: Santus
authors.forename: G
authors.initials: G
authors.lastname: Hüppe
authors.forename: J C
authors.initials: JC
authors.lastname: Mazière
authors.forename: L
authors.initials: L
authors.lastname: Dubertret
mesh_terms: D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005347:Fibroblasts / Q000201:enzymology / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000528:radiation effects*; D008315:Malondialdehyde / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013858:Thiobarbiturates / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D013858:Thiobarbiturates; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; C029684:thiobarbituric acid