Separation of carbohydrates and carbohydrate derivatives by HPLC with cation-exchange columns at high pH.
Cation-exchange columns were found to be stable when used at high pH and high temperature for high-performance liquid chromatographic separations of carbohydrates and carbohydrate derivatives. Pulsed amperometric detection and refractive index detection were found to be suitable detection modes with these cation-exchange columns. Significant differences in carbohydrate separation selectivity were observed between cation-exchange and anion-exchange columns.
Togami
D W
DW
Interaction Chemicals Inc., Mountain View, CA 94043.
Poulsen
B J
BJ
Batalao
C W
CW
Rolls
W A
WA
D002241:Carbohydrates / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D013696:Temperature
D002241:Carbohydrates