Enterostatin (Val-Pro-Asp-Pro-Arg), the activation peptide of procolipase, selectively reduces fat intake.
Valine-proline-aspartate-proline-arginine (VPDPR), the amino terminal pentapeptide of pancreatic procolipase, produced a dose-dependent reduction in food intake when injected intraperitoneally into Osborne-Mendel rats that had been starved overnight. This inhibition of feeding was observed when the rats were fed a high-fat diet but not in rats fed a high-carbohydrate, low-fat diet. At higher doses of VPDPR, the inhibition of feeding was maintained for over 6 hours. An equimolar mixture of the free amino acids had no effect on food intake. In rats adapted to a three-choice macronutrient diet, VPDPR inhibited fat intake but had no effect on carbohydrate or protein intake. This selective inhibition of fat intake was observed in both overnight-fasted rats presented with food and in ad-lib-fed rats at the beginning of the dark-onset feeding period. It is suggested that this peptide may be a feedback signal to regulate the intake of dietary fat.
Okada
S
S
Pennington Biomedical Research Center, Baton Rouge, LA 70808.
York
D A
DA
Bray
G A
GA
Erlanson-Albertsson
C
C
D000818:Animals; D001835:Body Weight / Q000502:physiology; D002940:Circadian Rhythm / Q000502:physiology; D003089:Colipases / Q000502:physiology*; D004041:Dietary Fats / Q000008:administration & dosage*; D004789:Enzyme Activation / Q000502:physiology; D004792:Enzyme Precursors; D005247:Feeding Behavior / Q000502:physiology; D005518:Food Preferences / Q000502:physiology*; D011498:Protein Precursors / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
D003089:Colipases; D004041:Dietary Fats; D004792:Enzyme Precursors; D011498:Protein Precursors; C037107:procolipase