Maintenance of CNS tissues in vitro for subsequent pharmacologic evaluation: a simple and inexpensive superfusion chamber.
A new simple, inexpensive holding chamber is described for maintaining brain slices in a viable condition for long periods of time. The advantages of its superfusion-type operation and application of this chamber to in vitro electrophysiologic studies of the pharmacologic action of serotonin in the midbrain dorsal raphe nucleus are discussed.
Black
E W
EW
Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston 77550.
Lakoski
J M
JM
D000818:Animals; D002490:Central Nervous System / Q000187:drug effects*; D004594:Electrophysiology; D066298:In Vitro Techniques; D010477:Perfusion / Q000295:instrumentation*; D011903:Raphe Nuclei / Q000187:drug effects; D051381:Rats; D012701:Serotonin / Q000008:administration & dosage / Q000494:pharmacology
D012701:Serotonin