Post-transcriptional regulation in higher eukaryotes: the role of the reporter gene in controlling expression.
We have investigated whether reporter genes influence cytoplasmic regulation of gene expression in tobacco and Chinese hamster ovary (CHO) cells. Two genes, uidA encoding beta-glucuronidase (GUS) from Escherichia coli and Luc, encoding firefly luciferase (LUC), were used to analyze the ability of a cap, polyadenylated tail, and the 5'- and 3'-untranslated regions (UTR) from tobacco mosaic virus (TMV) to regulate expression. The regulation associated with the 5' cap structure and the TMV 5'-UTR, both of which enhance translational efficiency, was reporter gene-independent. The poly(A) tail and the TMV 3'-UTR, which is functionally equivalent to a poly(A) tail, increase translational efficiency as well as mRNA stability. The regulation associated with these 3' ends was highly reporter gene-dependent; their effect on GUS expression was almost an order of magnitude greater than that on LUC expression. In tobacco, the tenfold reporter gene effect on poly(A) tail or TMV 3'-UTR function could not be explained by a differential impact on mRNA stability; GUS and LUC mRNA half-life increased only twofold when either the poly(A) tail or TMV 3'-UTR was present. In CHO cells, however, GUS mRNA was stabilized to a greater extent by a poly(A) tail or the TMV 3'-UTR than was LUC mRNA.
Gallie
D R
DR
Department of Biological Sciences, Stanford University, CA 94305-5020.
Feder
J N
JN
Schimke
R T
RT
Walbot
V
V
D000818:Animals; D001483:Base Sequence; D006224:Cricetinae; D003593:Cytoplasm / Q000502:physiology; D005819:Genetic Markers; D005966:Glucuronidase / Q000096:biosynthesis; D006207:Half-Life; D008156:Luciferases / Q000096:biosynthesis; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D010957:Plasmids; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism; D012808:Sigma Factor / Q000502:physiology; D014026:Nicotiana; D014027:Tobacco Mosaic Virus
D005819:Genetic Markers; D012333:RNA, Messenger; D012808:Sigma Factor; D008156:Luciferases; D005966:Glucuronidase