Effects of detergents on the sulfation of chondroitin sulfate by sulfotransferase from chicken embryo epiphyseal cartilage.
Homogenates of chicken embryo epiphyseal cartilage were prepared in buffered saline. The bulk of the sulfotransferase was found in the supernatant. However, small amounts of sulfotransferase were consistently found in the particulate fraction. Detergents (Triton X-100 and C12E8) added to the incubation mixture activated the sulfation of exogenous sulfate acceptor by the particulate fraction, whereas detergent treatment during homogenization increased sulfotransferase activity in the supernatant at the expense of that in the particulate fraction. Since sulfotransferase activities of the supernatant and particulate fractions had similar properties concerning specificity, affinity for chondroitin with different degrees of sulfation, thermal stability and activation by protamine, we conclude that the same enzyme is present in both fractions and that detergent activates indirectly, by releasing it to the medium.
Salac
M L
ML
Departamento de Bioquimica, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Brazil.
Mourão
P A
PA
D000818:Animals; D002642:Chick Embryo; D002809:Chondroitin Sulfates / Q000378:metabolism*; D003902:Detergents / Q000494:pharmacology*; D006132:Growth Plate / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D007700:Kinetics; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011479:Protamines / Q000494:pharmacology; D012995:Solubility; D015238:Sulfotransferases / Q000187:drug effects / Q000378:metabolism*
D003902:Detergents; D011479:Protamines; D011092:Polyethylene Glycols; D017830:Octoxynol; D002809:Chondroitin Sulfates; D015238:Sulfotransferases