The synthetic peptide from HIV increases functional activity of granulocytes in healthy subjects.
The influence of HIV lysate and eight synthetic peptides which are fragments of HIV proteins on the functional activity of polymorphonuclear neutrophils (PMN) was tested in 12 healthy subjects. PMN activity in nitroblue tetrazolium reduction (NBT test) and PMN chemiluminescence (CL) was studied. Only one peptide was found to result in a significant increase in NBT test on the whole blood. This was the oligopeptide (G-97) from the CD4-binding site of HIV-1 gp120. The increase of CL response of PMN in the presence of G-97 was revealed after only 15 min preincubation. The same effect in the presence of sera from healthy or infected patients at the persistent generalized lymphadenopathy stage was achieved by increasing the time of preincubation to 30 min. G-97 did not influence the proliferative activity of lymphocytes.
Gabrilovich
D I
DI
Laboratory of Epidemiology and Prevention of AIDS, Central Institute of Epidemiology USSR Ministry of Public Health, Moscow.
Kozhich
A T
AT
Rosly
I M
IM
Suvorova
Z K
ZK
Ivanov
V S
VS
Moshnikov
S A
SA
Tchikin
L D
LD
Melichov
V I
VI
Pokrovsky
V V
VV
D000328:Adult; D002478:Cells, Cultured; D005260:Female; D006098:Granulocytes / Q000276:immunology* / Q000378:metabolism; D015488:HIV Antigens / Q000138:chemical synthesis / Q000276:immunology*; D006801:Humans; D008163:Luminescent Measurements; D008213:Lymphocyte Activation; D008297:Male; D009580:Nitroblue Tetrazolium / Q000378:metabolism
D015488:HIV Antigens; D009580:Nitroblue Tetrazolium