Difficulties of molecular dissociation of Clostridium botulinum type G progenitor toxin.
Clostridium botulinum type G progenitor toxin was chromatographed on DEAE-Sephadex and Q-Sepharose equilibrated with 0.05 M Tris-HCl buffer, pH 8.0, containing 0.2 M urea. The toxin was eluted in a single protein peak from DEAE-Sephadex, but it was eluted in four protein peaks from Q-Sepharose; the third peak was toxic and the others were nontoxic. The third peak, appearing to be the toxic component, had a molecular mass of 150,000. In SDS-polyacrylamide gel electrophoresis, purified type G progenitor toxin migrated in six bands, with molecular masses of 150,000, 140,000, 58,000, 10,800, 10,600, and 10,400. Type G progenitor toxin may be composed of a toxin component with a molecular mass of 150,000 and a nontoxic component in a manner similar to progenitor toxins of other types. Type G toxic component, whether it was reduced or not, migrated in a single band to the same relative positions in SDS-PAGE; type A toxic component reduced with 2-mercaptoethanol migrated in two bands.
Nukina
M
M
Public Health Research Institute of Kobe City, Hyogo, Japan.
Mochida
Y
Y
Sakaguchi
S
S
Sakaguchi
G
G
D001905:Botulinum Toxins / Q000737:chemistry* / Q000633:toxicity; D002847:Chromatography, Agarose; D002852:Chromatography, Ion Exchange; D003014:Clostridium botulinum / Q000032:analysis* / Q000472:pathogenicity; D004229:Dithiothreitol; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D046911:Macromolecular Substances; D008970:Molecular Weight
D046911:Macromolecular Substances; D001905:Botulinum Toxins; D004229:Dithiothreitol