Hydrophobic membrane thickness and lipid-protein interactions of the leucine transport system of Lactococcus lactis.
The effect of the phospholipid acyl chain carbon number on the activity of the branched-chain amino acid transport system of Lactococcus lactis has been investigated. Major fatty acids identified in a total lipid extract of L. lactis membranes are palmitic acid (16:0), oleic acid (18:1) and the cyclopropane-ring containing lactobacillic acid (19 delta). L. lactis membrane vesicles were fused with liposomes prepared from equimolar mixtures of synthetic phosphatidylethanolamine (PE) and phosphatidylcholine (PC) with cis mono-unsaturated acyl chains. The activity of the branched-chain amino acid carrier is determined by the bulk properties of the membrane (Driessen, A.J.M., Zheng, T., In 't Veld, G., Op den Kamp, J.A.F. and Konings, W.N. (1988) Biochemistry 27, 865-872). PE acts as an activator and PC is ineffective. Counterflow and protonmotive-force driven transport of leucine is sensitive to changes in the acyl chain carbon number of both phospholipids and maximal with dioleoyl-PE/dioleoyl-PC. Above the gel to liquid-crystalline phase transition temperature of the lipid species, membrane fluidity decreased with increasing acyl chain carbon number. Our data suggest that the carbon number of the acyl chains of PE and PC determine to a large extent the activity of the transport system. This might be relevant for the interaction of PE with the transport protein. Variations in the acyl chain composition of PC exert a more general effect on transport activity. The acyl chain composition of phospholipids determines the membrane thickness (Lewis, B.A. and Engelman, D.M. (1983) J. Mol. Biol. 166, 211-217). We therefore propose that the degree of matching between the lipid-bilayer and the hydrophobic thickness of the branched-chain amino acid carrier is an important parameter in lipid-protein interactions.
In 't Veld
G
G
Department of Microbiology, University of Groningen, Haren, The Netherlands.
Driessen
A J
AJ
Op den Kamp
J A
JA
Konings
W N
WN
D001692:Biological Transport; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure*; D002463:Cell Membrane Permeability; D004161:Diphenylhexatriene / Q000031:analogs & derivatives; D005227:Fatty Acids / Q000032:analysis; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D005614:Freeze Fracturing; D007700:Kinetics; D013294:Lactococcus lactis / Q000378:metabolism* / Q000648:ultrastructure; D007930:Leucine / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism; D008560:Membrane Fluidity; D008854:Microscopy, Electron; D019301:Oleic Acid; D009829:Oleic Acids / Q000032:analysis; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000032:analysis; D010713:Phosphatidylcholines / Q000032:analysis / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000032:analysis / Q000378:metabolism
D005227:Fatty Acids; D005456:Fluorescent Dyes; D008081:Liposomes; D009829:Oleic Acids; D010169:Palmitic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D004161:Diphenylhexatriene; D019301:Oleic Acid; D019308:Palmitic Acid; C033628:1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene; D007930:Leucine; C028716:phytomonic acid