Regulation of HLA class I gene expression in human colorectal carcinoma.
The under-representation of HLA antigens in human tumors is usually associated with a poor prognosis. In this report, the expression of HLA class I genes in human colorectal carcinomas was studied using HLA-A and HLA-B locus-specific probes. Over 50% of the colorectal carcinomas studied showed a reduction in the amount of steady state HLA class I mRNA. For some carcinomas, non-coordinated regulation of the HLA-A and HLA-B genes was observed. The mechanism of HLA suppression was investigated and is most likely due to the presence of transcriptional regulatory elements.
Soong
T W
TW
Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore.
Oei
A A
AA
Hui
K M
KM
D015152:Blotting, Northern; D015139:Blotting, Southern; D002272:Carcinoembryonic Antigen / Q000096:biosynthesis; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D003110:Colonic Neoplasms / Q000235:genetics / Q000276:immunology*; D015536:Down-Regulation; D005260:Female; D015234:HLA-A Antigens / Q000235:genetics / Q000378:metabolism; D015235:HLA-B Antigens / Q000235:genetics / Q000378:metabolism; D006649:Histocompatibility Antigens / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008297:Male; D008745:Methylation; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012004:Rectal Neoplasms / Q000235:genetics / Q000276:immunology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D002272:Carcinoembryonic Antigen; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006649:Histocompatibility Antigens; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D007371:Interferon-gamma; D015500:Chloramphenicol O-Acetyltransferase