Immunohistochemical detection of chlamydiae in formalin-fixed tissue sections: comparison of a monoclonal antibody with yolk derived antibodies (IgY).
Immunohistological detection of chlamydiae in formalin-fixed and paraffin-embedded sections of various organs from several species is described. In a retrospective study, two antisera, a commercially available monoclonal murine antibody (IgMur) and vitelline immunoglobulins (IgY), extracted from the egg yolk of immunized hens, were compared and tested for their applicability under routine condition. Both antisera were applied to tissues from which chlamydiae had been isolated or in which the presence of chlamydiae had been suspected in specially stained sections. Antigen labelling was optimal with the monoclonal antibody. Vitelline immunoglobulins produced some unspecific reactions, especially in lung tissue sections. Because of the antigenic relationship between the vitelline antibodies and tissues of birds, IgY are not suitable for the detection of psittacosis on avian substrates, when using an indirect immunological method. Staining in other tissues e.g. intestine or placenta was of equal quality as that attained with monoclonal antibodies. Depending on the advantages and disadvantages in every individual case, one of the two antibodies may be chosen for further studies. Vitelline antibodies should be preferred with respect to animal welfare.
Kunz
U S
US
Department of Veterinary Pathology, University of Zurich.
Pospischil
A
A
Paccaud
M F
MF
D000818:Animals; D000911:Antibodies, Monoclonal; D002645:Chickens; D002689:Chlamydia / Q000302:isolation & purification*; D002690:Chlamydia Infections / Q000382:microbiology*; D004530:Egg Yolk; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins; D007150:Immunohistochemistry
D000911:Antibodies, Monoclonal; C016512:IgY; D007136:Immunoglobulins