Detection of gelatinolytic enzyme activities after sodium dodecyl sulfate-electrophoresis and protein blotting.
Visualization of proteases with gelatinolytic activity in sodium dodecyl sulfate gels is described. After conventional sodium dodecyl sulfate-polyacrylamide gel electrophoresis, proteins are transferred onto nitrocellulose membranes preincubated with 0.3% gelatine. During the protein electrotransfer, the proteases are renaturated and their enzymatic activity is restored. After nonspecific protein staining, bands with proteolytic activity appear as white areas on a dark background.
Moos
J
J
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
D000176:Acrosin / Q000737:chemistry; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases; D005780:Gelatin / Q000378:metabolism*; D006868:Hydrolysis; D008297:Male; D012967:Sodium Dodecyl Sulfate; D013552:Swine
D012967:Sodium Dodecyl Sulfate; D005780:Gelatin; D010450:Endopeptidases; D000176:Acrosin