title: Expression of D-mannose binding sites on human spermatozoa: comparison of fertile donors and infertile patients.
abstract: OBJECTIVE
  The hypothesis that defective expression of D-mannose binding sites (presumptive elements of the sperm-zona pellucida binding mechanism) is related to male infertility was tested.
  
  
  METHODS
  Experiments were performed on sperm samples from two groups of men classified, respectively, as fertile and infertile, based on their reproductive history.
  
  
  METHODS
  The study was carried out in an andrologic laboratory of a University Hospital.
  
  
  METHODS
  Fertile men were healthy sperm donors; infertile men were patients with fertility problems.
  
  
  METHODS
  D-mannose binding sites were visualized by fluorescence microscopy using a mannosylated neoglycoprotein probe.
  
  
  METHODS
  The hypothesis as reported in the objective section was formulated before data collection and was not modified thereafter.
  
  
  RESULTS
  Sperm from fertile men displayed a characteristic pattern of changes in the expression of D-mannose binding sites during in vitro capacitation. This pattern was altered in sperm from infertile men.
  
  
  CONCLUSIONS
  If the relationship between defective expression of D-mannose binding sites and decreased sperm fertilizing ability is validated by parallel tests of sperm-zona binding, it may be used for development of chemical tests replacing the current ones using human zonae pellucidae.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM) U-187, Clamart, France.
authors.forename: J
authors.initials: J
authors.lastname: Tesarik
authors.forename: C
authors.initials: C
authors.lastname: Mendoza
authors.forename: A
authors.initials: A
authors.lastname: Carreras
mesh_terms: D001665:Binding Sites; D005404:Fixatives; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism*; D008297:Male; D008358:Mannose / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D012709:Serum Albumin; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013075:Sperm Capacitation; D013094:Spermatozoa / Q000378:metabolism*; D013861:Thiocyanates
chemical_list: D005404:Fixatives; D005452:Fluoresceins; D005456:Fluorescent Dyes; D012709:Serum Albumin; D013861:Thiocyanates; C023840:mannose-bovine serum albumin conjugate; D012710:Serum Albumin, Bovine; D016650:Fluorescein-5-isothiocyanate; D008358:Mannose