title: Change in the amount of epsilon-hexosyllysine, UV absorbance, and fluorescence of collagen with age in different animal species.
abstract: Skin and aorta collagen specimens of Wistar rats, white mice, beagle dogs, cats, horses, and human necropsies of different ages were examined with respect to the content of glycated products. The data presented show that (a) glycation and accumulation of the chromophore(s) are comparable in collagen samples from different species of comparable age; (b) glycation and pigmented accumulation increase markedly during the first 5-10 years of age; (c) the extent of glycation is different in different tissues (in particular, glycation of aortal collagen is about twice that of skin collagen); and (d) collagen pigmentation as followed by fluorescence is comparable in aortal and skin collagen (except below 10 years); pigmentation measured by absorbance at 350 nm is, on the contrary, lower in aortal than in skin collagen. Based on the assumption of constant blood glucose level during the life span, it appears feasible to conclude that the degree of nonenzymatic collagen glycation reflects the time period for which the protein was exposed to the action of sugars. This period, because of increased cross-linking, is likely to be extended in older animals. Other factors, such as differences in collagen turnover between different tissues and the intensity of the removal process of the glycated products, should be taken into consideration as well.
authors.affiliation: Institute of Physiology, Czechoslovak Academy of Sciences, Prague.
authors.forename: I
authors.initials: I
authors.lastname: Miksík
authors.forename: Z
authors.initials: Z
authors.lastname: Deyl
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000378:metabolism; D002415:Cats; D002846:Chromatography, Affinity; D003094:Collagen / Q000378:metabolism*; D004285:Dogs; D005662:Furaldehyde / Q000031:analogs & derivatives / Q000378:metabolism; D006031:Glycosylation; D006736:Horses; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism; D051379:Mice; D012867:Skin / Q000378:metabolism; D013045:Species Specificity; D013050:Spectrometry, Fluorescence; D013400:Sugar Acids / Q000378:metabolism*; D013858:Thiobarbiturates; D014466:Ultraviolet Rays
chemical_list: D013400:Sugar Acids; D013858:Thiobarbiturates; C008046:5-hydroxymethylfurfural; D003094:Collagen; D005662:Furaldehyde; D008239:Lysine; C029684:thiobarbituric acid