Abbott prism: a multichannel heterogeneous chemiluminescence immunoassay analyzer.
We describe a multichannel heterogeneous immunoassay analyzer in which a sample is split between disposable reaction trays in a group of linear tracks. The system's pipettor uses noninvasive sensing of the sample volume and disposable pipet tips. Each assay track has (a) a conveyor belt for moving reaction trays to predetermined functional stations, (b) temperature-controlled tunnels, (c) noncontact transfer of the reaction mixture between incubation and detection wells, and (d) single-photon counting to detect a chemiluminescence (CL) signal from the captured immunochemical product. A novel disposable reaction tray, with separate reaction and detection wells and self-contained fluid removal, is used in conjunction with the transfer device on the track to produce a carryover-free system. The linear immunoassay track has nine predetermined positions for performing individual assay steps. Assay step sequence and timing is selected by changing the location of the assay modules between these predetermined positions. The assay methodology, a combination of microparticle capture and direct detection of a CL signal on a porous matrix, offers excellent sensitivity, specificity, and ease of automation. Immunoassay configurations have been tested for hepatitis B surface antigen and for antibodies to hepatitis B core antigen, hepatitis C virus, human immunodeficiency virus I and II, and human T-cell leukemia virus I and II.
Khalil
O S
OS
Diagnostics Division, Abbott Laboratories, Abbott Park, IL 60064.
Zurek
T F
TF
Tryba
J
J
Hanna
C F
CF
Hollar
R
R
Pepe
C
C
Genger
K
K
Brentz
C
C
Murphy
B
B
Abunimeh
N
N
D001667:Binding, Competitive; D015483:HIV Antibodies / Q000032:analysis; D015481:HTLV-I Antibodies / Q000032:analysis; D015482:HTLV-II Antibodies / Q000032:analysis; D006512:Hepatitis B Core Antigens / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D008163:Luminescent Measurements; D012588:Scintillation Counting; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013972:Thyrotropin / Q000032:analysis
D015483:HIV Antibodies; D015481:HTLV-I Antibodies; D015482:HTLV-II Antibodies; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens; D013972:Thyrotropin