title: Multiple forms of NADPH-cytochrome P450 reductase in higher plants.
abstract: We report on the presence of multiple forms of NADPH-cyt P450 reductase in microsomes from higher plants. This contrasts with the animal cyt P450 monooxygenases, where the numerous cyt P450 isoforms are reduced by a single form of reductase. Three NADPH-cyt c reductases have been resolved from Jerusalem artichoke tuber microsomes by chromatography on Reactive Red Agarose and Concanavalin A-Sepharose. Their molecular weights, determined by sodium dodecylsulfate-gel electrophoresis, are 80,000, 82,000 and 84,000. The three proteins share common epitopes and are dependent upon FMN for catalytic activity. They are highly selective for NADPH as electron donor, and allowed effective reconstitution of trans-cinnamic acid and 3,9-dihydroxypterocarpan 6a-hydroxylase activities with purified cyt P450 fractions from Helianthus tuberosus and Glycine max, respectively. As such, they appear as true isoenzyme forms of NADPH-cyt P-450 reductase.
authors.affiliation: Laboratoire d'Enzymologie Cellulaire et Moléculaire, Institut de Biologie Moléculaire des Plantes, Strasbourg, France.
authors.forename: I
authors.initials: I
authors.lastname: Benveniste
authors.forename: A
authors.initials: A
authors.lastname: Lesot
authors.forename: M P
authors.initials: MP
authors.lastname: Hasenfratz
authors.forename: G
authors.initials: G
authors.lastname: Kochs
authors.forename: F
authors.initials: F
authors.lastname: Durst
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005486:Flavin Mononucleotide / Q000378:metabolism; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology; D008970:Molecular Weight; D009251:NADPH-Ferrihemoprotein Reductase / Q000302:isolation & purification / Q000378:metabolism*; D010944:Plants / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; D005486:Flavin Mononucleotide; D009251:NADPH-Ferrihemoprotein Reductase