Bone-inducing implants in head and neck surgery: an experimental study.
The use of autologous bone for head and neck reconstruction requires a separate harvesting procedure which provides limited quantities of bone that may become infected or undergo resorption after being implanted. In this study, a collagen/ceramic carrier containing osteoinductive factor extract (OFE) was used in a rabbit facial augmentation model. Bone-inducing activity of these implants were evaluated in subcutaneous, intramuscular, and subperiosteal sites. Implants with (test) and without OFE (control) were placed on opposite sides of the face in 40 rabbits, and were harvested at 21 days. Bone formation was evaluated by implant alkaline phosphatase determinations and histomorphometry. Osteoblastic activity, bone formation, and preservation of facial augmentation were noted in the OFE implants, showing maximal bone formation when implanted subperiosteally. Control (no OFE) and demineralized bone implants showed no bone formation. Before these implants can be used clinically, novel bone-inducing factors must be manufactured by recombinant deoxyribonucleic acid (DNA) methodology to verify activity of the homogeneous molecule which would be free of other proteins or infectious agents.
Toriumi
D M
DM
Department of Otolaryngology-Head and Neck Surgery, University of Illinois College of Medicine, Chicago 60612.
East
C A
CA
Rosen
D M
DM
Chu
G
G
Liu
C C
CC
Larrabee
W F
WF
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002113:Calcification, Physiologic; D002516:Ceramics; D003094:Collagen; D005147:Facial Bones / Q000201:enzymology / Q000473:pathology / Q000601:surgery*; D006023:Glycoproteins / Q000008:administration & dosage*; D006133:Growth Substances / Q000008:administration & dosage*; D019736:Prostheses and Implants; D011817:Rabbits
D006023:Glycoproteins; D006133:Growth Substances; D003094:Collagen; D000469:Alkaline Phosphatase