[Assessment of conformation changes of immunoglobulin G molecules using a fluorescent probe].
A proximate method for conformational changes detection in immunoglobulin G preparations used in research and medical practice is introduced. This method is based on the measurement of IgG-connected 1,8-anilinenaphtalenesulphonate fluorescent probe (ANS) time of damping. When the protein structure is broken fluorescence damping time of IgG-connected ANS rises up.
Pupyrev
N P
NP
Matveeva
N A
NA
Naumenko
V Iu
VIu
Karpov
F F
FF
D000817:Anilino Naphthalenesulfonates / Q000737:chemistry; D005456:Fluorescent Dyes; D007074:Immunoglobulin G / Q000737:chemistry*; D011487:Protein Conformation
D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; C027132:1-anilino-8-naphthalenesulfonate
Otsenka konformatsionnykh izmeneniĭ molekul immunoglobulina G s pomoshch'iu fluorestsentnogo zonda.