title: Quality control testing of surfaces for mammalian cell culture using cells propagated in a protein-free medium.
abstract: Mouse NCTC clone 929 L (L-929) cells were propagated continuously for 3 years as monolayers in a protein-free chemically-defined medium. These cells, designated L-929-WS, were used for quality control testing of the surfaces of commercially available cell culture plastic flasks. Differences in attachment and saturation density of L-929-WS cells in a protein-free culture medium were taken to define various levels of quality of the culture vessels tested. The rate of attachment and growth of L-929-WS cells on a surface of a given quality correlated directly with that of human embryonal fibroblasts and embryonal epithelial cells grown in a serum-free medium supplemented with growth factors and hormones. L-929-WS cells propagated continuously in a protein-free medium provide a simple and sensitive assay system for more general quality control testing of surfaces used for the culture of monolayer cells.
authors.affiliation: Centre of Hygiene, Department of Virology, Clinics of the J.W. Goethe University Frankfurt a. M., Federal Republic of Germany.
authors.forename: J
authors.initials: J
authors.lastname: Cinatl
authors.forename: J
authors.initials: J
authors.lastname: Cinatl
authors.forename: H
authors.initials: H
authors.lastname: Rabenau
authors.forename: J W
authors.initials: JW
authors.lastname: Ahn
authors.forename: H W
authors.initials: HW
authors.lastname: Doerr
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002999:Clone Cells / Q000166:cytology*; D003470:Culture Media; D003584:Cytological Techniques / Q000295:instrumentation / Q000592:standards*; D006801:Humans; D011786:Quality Control; D013499:Surface Properties
chemical_list: D003470:Culture Media