title: DNase I-hypersensitive sites and transcription factor-binding motifs within the mouse E beta meiotic recombination hot spot.
abstract: The second intron of the E beta gene in the mouse major histocompatibility complex is the site of a meiotic recombination hot spot. We detected two DNase I-hypersensitive sites in this intron in meiotic cells isolated from mouse testes. One site appears to be constitutive and is found in other tissues regardless of whether or not they express the E beta gene. Near this hypersensitive site are potential binding motifs for H2TF1/KBF1, NF kappa B, and octamer transcription factors. Gel retardation studies with mouse lymphoma cell nuclear extracts confirmed that each of these motifs is capable of binding protein. The binding of transcription factors may contribute to the enhancement of recombination potential by altering chromatin structure and increasing the accessibility of the DNA to the recombination machinery.
authors.affiliation: Molecular Biology Section, University of Southern California, Los Angeles 90089-1340.
authors.forename: R
authors.initials: R
authors.lastname: Shenkar
authors.forename: M H
authors.initials: MH
authors.lastname: Shen
authors.forename: N
authors.initials: N
authors.lastname: Arnheim
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D003850:Deoxyribonuclease I / Q000378:metabolism*; D005260:Female; D005796:Genes; D005802:Genes, MHC Class II; D007438:Introns; D008297:Male; D008540:Meiosis; D008745:Methylation; D051379:Mice; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D015183:Restriction Mapping; D013737:Testis / Q000166:cytology / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D014157:Transcription Factors; D003850:Deoxyribonuclease I