Galactosyltransferase activity is restricted to the plasma membranes of equine and bovine sperm.
beta 1, 4-Galactosyltransferase (GalTase) is localized to the plasma membrane of mouse sperm, in which it mediates the binding of sperm to glycoconjugate residues in the egg zona pellucida. In this study, the presence of subcellular distribution of sperm GalTase were determined in two other mammalian species that yield sufficient sperm for subcellular fractionation. Equine and bovine semen were collected, and the plasma membranes (PM), outer acrosomal membranes (OAM), and inner acrosomal membranes (IAM) were sequentially removed. The purities of the isolated membrane preparations were determined by transmission electron microscopy and found to be greater than or equal to 90%, 96%, and 98% for equine PM, OAM, and IAM, respectively, and greater than or equal to 80%, 94%, and 97% for bovine PM, OAM, and IAM, respectively. GalTase activity was assayed under optimal conditions in all membrane preparations and was preferentially localized to the isolated PM both in equine and in bovine spermatozoa. The selective localization of GalTase to the sperm PM in two other species suggest that it may serve as a generalized gamete receptor during initial sperm-egg binding in mammals.
Fayrer-Hosken
R A
RA
Department of Large Animal Medicine; College of Veterinary Medicine, University of Georgia, Athens 30602.
Caudle
A B
AB
Shur
B D
BD
D000818:Animals; D002417:Cattle / Q000378:metabolism*; D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D005700:Galactosyltransferases / Q000378:metabolism*; D006736:Horses / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008854:Microscopy, Electron; D013094:Spermatozoa / Q000201:enzymology* / Q000648:ultrastructure
D005700:Galactosyltransferases