Radio-immuno binding test for anti-sperm antibody detection: analysis and critical revision of various methodological steps.
The authors report the results of a methodological study to optimize a radioimmunoassay to detect anti-sperm antibodies. They describe here the definitive methodology with all the data relating to the laboratory trials conducted at each step of the technique. Results seem to demonstrate that this radioimmunological method is sufficiently simple, specific, sensitive and reliable for use in a highly specialized laboratory of reproductive immunology. Furthermore, this type of analytical approach, whilst indicating once more the difficulty of the technical study of anti-sperm autoimmunity, confirms (with concrete data) the necessity of being extremely "prudent" in evaluating the results of this kind of anti-sperm antibody test.
Gandini
L
L
Laboratory of Immunology of Reproduction and Seminology, University of Rome La Sapienza, Italy.
Lombardo
F
F
Lenzi
A
A
Dondero
F
F
D000372:Agglutination Tests; D000906:Antibodies / Q000097:blood*; D000888:Antibodies, Anti-Idiotypic; D000941:Antigens / Q000276:immunology; D001323:Autoantibodies / Q000097:blood; D006801:Humans; D008297:Male; D011863:Radioimmunoassay / Q000379:methods*; D013094:Spermatozoa / Q000276:immunology*; D013205:Staphylococcal Protein A; D013696:Temperature
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D001323:Autoantibodies; D013205:Staphylococcal Protein A