Suitable survival and half-life of red cells after frozen storage in excess of 10 years.
To examine the appropriateness of the Food and Drug Administration's 10-year storage time for previously frozen red cells, 24-hour posttransfusion survival studies were performed, and the half-life of 3 units of autologous red cells that had been stored for 13.5, 14, and 17 years, respectively, was measured. The units had acceptable freeze-thaw-wash recovery (83.3-91.4%). When a 51Cr label was used for the previously frozen red cells and a simultaneous 52Cr label for freshly drawn autologous red cells was used as a comparison, it was seen that the previously frozen cells had normal 24-hour posttransfusion survival (75.1-88.4%) as well as normal half-life (23-33.7 days). These findings support further extension of the maximum allowable storage time for previously frozen red cells.
Umlas
J
J
Department of Pathology, Mount Auburn Hospital, Cambridge, Massachusetts.
Jacobson
M
M
Kevy
S V
SV
D001793:Blood Preservation; D001804:Blood Transfusion, Autologous; D015925:Cryopreservation; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000502:physiology*; D006207:Half-Life; D006801:Humans; D013997:Time Factors