An in vitro bacterial model of cytotoxicity to living cells caused by dopamine and 6-hydroxydopamine oxidation at physiological pH.
The cytotoxicity of dopamine (DA) and 6-hydroxydopamine (6-OHDA) on living cells, in vitro, has been previously deeply investigated in neuroblastoma cells. This study was designed to explore the possibility to use bacteria as targets for studying DA and 6-HODA cytotoxicity. Both DA and 6-HODA oxidize when added to bacteriological media. The rate of autoxidation of 6-HODA was greater than DA within the first hours. The oxidation-dependent cytotoxicity caused bacterial growth-inhibition and killing at concentration of 10(-4)M. All the bacterial strains tested were slightly more susceptible to DA than to 6-HODA. Antioxidants (sodium metabisulfite, cysteine) prevented the oxidation and abolished the growth-inhibitory activity. The addition of exogenous catalase protected the cells against the effect of the oxidation of both the catecholamines up to the concentration of 5 mM, while the addition of exogenous superoxide dismutase protected the cells only at the minimal inhibitory concentrations. Taking into account that some of the results obtained are similar to those previously reported using neuroblastoma cells as targets, the use of bacteria for studying oxygen toxicity from these catecholamines seems to be a potentially useful model system.
Giunta
S
S
Laboratorio Clinico, I.N.R.C.A. Sede di Roma, Italy.
Galeazzi
L
L
Groppa
G
G
D001419:Bacteria / Q000187:drug effects*; D002374:Catalase / Q000633:toxicity; D004298:Dopamine / Q000633:toxicity*; D016166:Free Radical Scavengers; D006863:Hydrogen-Ion Concentration; D006892:Hydroxydopamines / Q000633:toxicity*; D008826:Microbial Sensitivity Tests; D008954:Models, Biological; D016627:Oxidopamine; D010100:Oxygen / Q000633:toxicity; D013482:Superoxide Dismutase / Q000633:toxicity
D016166:Free Radical Scavengers; D006892:Hydroxydopamines; D016627:Oxidopamine; D002374:Catalase; D013482:Superoxide Dismutase; D010100:Oxygen; D004298:Dopamine