Identification of Treponema hyodysenteriae and Treponema innocens using two four-hour identification systems.
Two 4-hour identification systems, the RapID-ANAII (Innovative Diagnostic Systems) and the ANI card (Vitek Systems), were used to identify isolates of Treponema hyodysenteriae and T. innocens. Twenty-one isolates of T. innocens and 53 isolates of T. hyodysenteriae were tested with both systems. With the ANI system, alpha-galactosidase was the only test that differentiated the two species. With the RapID-ANAII system, alpha-galactosidase and indole tests allowed differentiation of the two species. Treponema hyodysenteriae was alpha-galactosidase negative and indole positive, whereas T. innocens produces the opposite reactions. Three isolates were alpha-galactosidase negative and indole negative. These isolates represent a group intermediate between the two officially described species. The two species of swine Treponema can be identified by commercial identification kits and a third group of isolates intermediate between the two species was identified.
Achacha
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M
Groupe de Recherches sur les Maladies Infectieuses du Porc, Faculté de Médecine vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada.
Messier
S
S
D000818:Animals; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014208:Treponema / Q000302:isolation & purification*; D014211:Treponemal Infections / Q000382:microbiology / Q000662:veterinary*
D011933:Reagent Kits, Diagnostic