The macaque model for in vitro fertilization: superovulation techniques and ultrasound-guided follicular aspiration.
Prior methods for macaque in vitro fertilization (IVF) have incorporated laparoscopy and/or laparotomy as the primary means for oocyte recovery. Sonographic techniques, as used with human IVF, have been applied to the macaque, both for monitoring the response to hyperstimulation and for follicular aspiration prior to ovulation. Pergonal (hMG) was administered for 7 or 8 days beginning on cycle day 1 or 2 or for 6 days beginning on cycle day 3. This was followed by Pregnyl (hCG) prior to follicular aspiration. The quality of oocytes recovered from the 6-day treatment group was considerably better than those treated for greater than or equal to 7 days. It was concluded that ultrasound can provide a reliable means for documenting the response to ovarian stimulation and the successful transabdominal aspiration of multiple follicles.
VandeVoort
C A
CA
California Primate Research Center, University of California, Davis.
Tarantal
A F
AF
D000818:Animals; D004970:Estrone / Q000652:urine; D005260:Female; D005307:Fertilization in Vitro; D008252:Macaca fascicularis / Q000502:physiology*; D009865:Oocytes / Q000502:physiology; D006080:Ovarian Follicle / Q000166:cytology / Q000000981:diagnostic imaging*; D010053:Ovary / Q000000981:diagnostic imaging; D013396:Suction / Q000662:veterinary; D013480:Superovulation; D014463:Ultrasonography; D014599:Uterus / Q000000981:diagnostic imaging
D004970:Estrone