Genetic mapping by single sperm typing.
The polymerase chain reaction makes it possible to analyse DNA sequences in a single cell and has led to a new approach for constructing genetic maps. We describe a procedure called 'sperm typing' which can accurately classify individual meiotic products as recombinant of non-recombinant. This permits the linkage relationships among DNA polymorphisms to be determined without pedigree analysis.
Arnheim
N
N
Department of Biological Sciences, University of Southern California, Los Angeles 90089-1340.
Li
H
H
Cui
X
X
D002874:Chromosome Mapping / Q000379:methods*; D005914:Globins / Q000235:genetics; D006801:Humans; D008297:Male; D010281:Parathyroid Hormone / Q000235:genetics; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D013094:Spermatozoa / Q000166:cytology*
D010281:Parathyroid Hormone; D005914:Globins