Production of polyclonal and monoclonal antibodies for specific detection of nitrosation-proficient denitrifying bacteria in biological fluids.
Polyclonal and monoclonal antibodies were raised against the nitrosating enzyme previously isolated and purified from a denitrifying bacteria Pseudomonas aeruginosa. Using the polyclonal antibodies, a preliminary ELISA test was set up which allowed the detection of the nitrosating enzyme in 2 ml urine samples with a minimal total bacteria count of greater than or equal to 10(5) cells/ml. The use of such a rapid immunological screening test in clinical settings should ascertain whether individual subjects at higher risk for cancer of the stomach or bladder harbour more nitrosation-proficient microorganisms in their microflora. The availability of monoclonal antibodies provides a tool for studying the mechanisms of bacteria-mediated nitrosamine formation from precursor amines.
Venezia
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ND
International Agency for Research on Cancer, Lyon, France.
Calmels
S
S
Bartsch
H
H
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D001419:Bacteria / Q000201:enzymology / Q000302:isolation & purification*; D001826:Body Fluids / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D010088:Oxidoreductases / Q000032:analysis* / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000302:isolation & purification*; D011817:Rabbits / Q000276:immunology; D014556:Urine / Q000382:microbiology
D000906:Antibodies; D000911:Antibodies, Monoclonal; D010088:Oxidoreductases; C065724:nitrosating enzyme