[Expression of the glow worm luciferase gene in mammalian cells using vectors based on vaccinia viruses].
The transient expression of the two reporter genes, the genes for luciferase and bacterial beta-galactosidase, were used for comparative estimation of vaccinia viral promoters and for characterizing of the constructed plasmids. The recombinant clones of vaccinia virus expressing simultaneously and with high efficiency the luciferase and beta-galactosidase were used for studying the reproduction of vaccinia virus in mammalian cells. The advantages of the luciferase gene in using it as a reporter gene are discussed.
Krauzova
V I
VI
Kopylova-Sviridova
T N
TN
Timiriasova
T M
TM
Fodor
I I
II
D000818:Animals; D002478:Cells, Cultured; D001517:Coleoptera / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D008156:Luciferases / Q000235:genetics* / Q000378:metabolism; D008322:Mammals; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014162:Transfection; D014616:Vaccinia virus / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D008156:Luciferases; D001616:beta-Galactosidase
Ekspressiia gena liutsiferazy svetliachka v kletkakh mlekopitaiushchikhs ispol'zovaniem vektorov na osnove virusa ospovaktsiny.