31P and 3-fluoro-3-deoxy-D-glucose 19F in vivo NMR spectroscopy of aged rat brain.
31P and 3-fluoro-3-deoxy-D-glucose (3-FDG) 19F nuclear magnetic resonance (NMR) spectroscopic investigations were performed on aged rat brain in vivo. Phosphodiester (PDE) was found to be significantly higher (p less than 0.05) in aged brain (18 months, n = 10) than adult controls (6 months, n = 10) suggesting accelerated phospholipid catabolism in aged brain. Inorganic phosphate (Pi) levels were significantly lower (p less than 0.05) in aged brain (n = 5) than in normal adult controls (n = 5). The sorbitol index, a measure of aldose reductase activities in vivo, determined by the 3-FDG 19F NMR method, revealed that aldose reductase activities were significantly higher (p less than 0.05) in aged brain.
Kwee
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IL
Neurochemistry Research Laboratory, Department of Veterans Affairs Medical Center, Martinez, CA 94553.
Nakada
T
T
Suzuki
N
N
D000375:Aging / Q000378:metabolism*; D000449:Aldehyde Reductase / Q000032:analysis; D000818:Animals; D001921:Brain / Q000378:metabolism*; D003847:Deoxyglucose / Q000031:analogs & derivatives*; D009682:Magnetic Resonance Spectroscopy; D010761:Phosphorus Radioisotopes; D051381:Rats; D011919:Rats, Inbred Strains
D010761:Phosphorus Radioisotopes; C007253:3-deoxy-3-fluoro-D-glucose; D003847:Deoxyglucose; D000449:Aldehyde Reductase