Relationship between total magnesium concentration and free intracellular magnesium in sheep red blood cells.
The cellular free magnesium concentration of ionophore A23187 permeabilized high potassium sheep erythrocytes was measured by 31P nuclear magnetic resonance spectroscopy, and the total cellular magnesium concentration was determined by atomic absorption spectroscopy. The free versus total cellular magnesium concentrations yield a linear relationship on a log-log scale in the concentration range from 0.3 to 1.92 mmol Mg/liter cells. Thus, free intracellular magnesium concentrations can be calculated from atomic absorption data. The method permits the estimation of physiologically or experimentally induced variations of intracellular free magnesium concentrations between 7 and 405 microM magnesium in cell water. This range encompasses the free magnesium concentration of 335 +/- 60 microM in cell water determined for untreated erythrocytes.
Fujise
H
H
Department of Physiology and Biophysics, Wright State University School of Medicine, Dayton, OH 45435.
Cruz
P
P
Reo
N V
NV
Lauf
P K
PK
D000255:Adenosine Triphosphate / Q000097:blood; D000818:Animals; D002474:Cell-Free System; D004912:Erythrocytes / Q000378:metabolism*; D006454:Hemoglobins / Q000032:analysis; D008274:Magnesium / Q000097:blood*; D009682:Magnetic Resonance Spectroscopy; D012756:Sheep; D013054:Spectrophotometry, Atomic
D006454:Hemoglobins; D000255:Adenosine Triphosphate; C100003:deoxyhemoglobin; D008274:Magnesium