title: Filtration coefficient in isolated preserved and reperfused canine lung.
abstract: The filtration coefficient (Kf) in Starling's equation for fluid exchange was estimated in isolated reperfused canine lobes to evaluate the effect of ischemia-reperfusion injury on alveolar-capillary permeability quantitatively and to determine the inhibitory effects of a high dose of methylprednisolone (MPS) or dimethylthiourea (DMTU), a potent hydroxyl radical scavenger, on this injury. We reperfused isolated canine left lower lobes (LLLs) with blood at a constant flow after 3 hr of warm (38 degrees C) or cold (4 degrees C) ischemia and measured Kf after 1 hr of reperfusion. The mean value of Kf (+/- 1 SD) in the cold ischemic lobes (COLD, n = 7), 0.13 +/- 0.04 g.min-1.cmH2O-1.100 g-1, was not different from that in the control nonischemic lobes (CONT, n = 6), 0.10 +/- 0.04 g.min-1.cmH2O-1.100 g-1. In contrast, the mean value of the Kf in the warm ischemic lobes (WARM, n = 7), 0.38 +/- 0.17 g.min-1.cmH2O-1.100 g-1, was significantly (P less than 0.001) higher than in CONT.MPS (30 mg/Kg) or DMTU (0.75 g/kg) administered before isolation of LLL and before reperfusion reduced the increase in Kf in warm ischemic lobes to 0.19 +/- 0.09 and 0.19 +/- 0.05 g.min-1.cmH2O-1.100 g-1, respectively (P less than 0.005 WARM vs MPS, and P less than 0.01 WARM vs DMTU). MPS and DMTU also attenuated the impairment of gas exchange. We conclude that (1) reperfusion after 3 hr of warm ischemia increases Kf but after cold ischemia does not, and (2) MPS and DMTU prevent the increase in Kf.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: First Department of Surgery, Osaka University Medical School, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Matsumura
authors.forename: K
authors.initials: K
authors.lastname: Nakahara
authors.forename: S
authors.initials: S
authors.lastname: Miyoshi
authors.forename: T
authors.initials: T
authors.lastname: Mizuta
authors.forename: A
authors.initials: A
authors.lastname: Akashi
authors.forename: Y
authors.initials: Y
authors.lastname: Kawashima
mesh_terms: D000818:Animals; D002199:Capillary Permeability; D004285:Dogs; D005260:Female; D006439:Hemodynamics; D066298:In Vitro Techniques; D008168:Lung / Q000098:blood supply*; D008297:Male; D008775:Methylprednisolone / Q000494:pharmacology; D008955:Models, Cardiovascular; D009926:Organ Preservation; D010100:Oxygen / Q000097:blood; D011652:Pulmonary Circulation / Q000187:drug effects; D015424:Reperfusion; D013890:Thiourea / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: C038983:1,3-dimethylthiourea; D013890:Thiourea; D010100:Oxygen; D008775:Methylprednisolone