[Penetration of oligo/polynucleotides and their polyalkylating derivatives into rat cells transformed by simian adenovirus DNA].
The transport regularity of the [32P]-oligo/polynucleotides and their polyalkylating derivatives into SH2 rat cells transformed with SA7 adenovirus DNA was investigated. Derivatives penetrate the SH2 cells and their distribution in the subcellular fractions are proportional to the concentration of reagent in the medium. The transport efficiency of the derivatives is inhibited sharply with cell concentration increase and practically does not depend on the action of cell metabolism inhibitors. The data obtained assumes the mechanism of the derivatives transport to be liquid endocytosis. Being distributed in the cell components the polyalkylating derivatives were accumulated by the cell nuclei up to 10(5)-10(7) molecules per nucleus. Transport efficiency is much greater in the anchored cells than in the suspended ones. Though essential dephosphorylation of the utilized substances is observed in the SH2 cells, part of them maintain native chain length and the 5'-phosphate group after 1 h incubation in nucleic acids obtained from the cell nuclei.
Pantin
V I
VI
Sats
N V
NV
Surin
V L
VL
Egorov
L V
LV
Solov'ev
G Ia
GIa
Borovkova
T V
TV
Grineva
N I
NI
D000261:Adenoviruses, Simian; D000478:Alkylation; D000818:Animals; D001692:Biological Transport; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000378:metabolism*; D004705:Endocytosis; D009153:Mutagens / Q000493:pharmacokinetics; D009841:Oligonucleotides / Q000378:metabolism*; D051381:Rats
D004279:DNA, Viral; D009153:Mutagens; D009841:Oligonucleotides
Proniknovenie oligo/polinukleotidov i ikh poliakriliruiushchikh proizvodnykh v kletki krysy, trnasformirovannye DNK adenovirusa obez'ian.