Stimulation of Agrobacterium tumefaciens T-DNA transfer by overdrive depends on a flanking sequence but not on helical position with respect to the border repeat.
T-DNA transfer by Agrobacterium tumefaciens depends on the right border repeat of the T-DNA and is greatly stimulated by overdrive, an adjacent sequence. We report that the function of overdrive does not depend on helical position with respect to the border repeat. A synthetic 24-bp overdrive and a 12-bp region containing a fully conserved 8-bp core overdrive sequence stimulated virulence equally, but full function required additional bases to the left of the 24-bp sequence.
Shurvinton
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CE
Department of Agricultural Chemistry, Oregon State University, Corvallis 97331-6502.
Ream
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D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010935:Plant Diseases; D012091:Repetitive Sequences, Nucleic Acid; D012231:Rhizobium / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence
D004269:DNA, Bacterial; C036483:T-DNA