Comparison of mammalian, chicken and Xenopus brain-derived neurotrophic factor coding sequences.
We have used the polymerase chain reaction (PCR) to amplify and clone coding sequences of the mature region of brain-derived neurotrophic factor (BDNF) from monkey, rat, chicken and Xenopus genomic DNA. Consistent with previous reports, the predicted amino acid sequences obtained in this manner from monkey and rat were identical to other mammalian BDNF sequences. The chicken and Xenopus BDNF sequences are also highly conserved, but contain 7 and 8 amino acid substitutions, respectively, compared to mammalian BDNF. Comparison of these sequences with the homologous NGF and NT3 coding regions provides further insight into amino acid residues that may be responsible for the different receptor specificities of these factors.
Isackson
P J
PJ
Department of Anatomy and Neurobiology, University of California, Irvine 92717.
Towner
M D
MD
Huntsman
M M
MM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019208:Brain-Derived Neurotrophic Factor; D002645:Chickens / Q000235:genetics; D003001:Cloning, Molecular; D014644:Genetic Variation / Q000235:genetics*; D000882:Haplorhini / Q000235:genetics; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D020933:Neurotrophin 3; D016133:Polymerase Chain Reaction; D051381:Rats; D011917:Rats, Inbred Lew; D012689:Sequence Homology, Nucleic Acid; D014982:Xenopus laevis
D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D020933:Neurotrophin 3