Quantitative localization of human brain monoamine oxidase B by large section autoradiography using L-[3H]deprenyl.
The distribution of monoamine oxidase B (MAO-B) in the human brain was studied by quantitative autoradiography using L-[3H]deprenyl as a ligand. Two postmortem brains from patients without any known neurological diseases were used in this study. Cryosections of 100 microns thickness were taken on tape/paper and transferred to gelatinized glass plates. The sections were incubated with 10 nM L-[3H]deprenyl for 1 h and exposed to a film at 4 degrees C for 4 weeks. The autoradiograms were analyzed by computerized densitometry. High L-[3H]deprenyl binding was observed in caudate nucleus, putamen, cingulate gyrus and insula cortex. Moderate to low binding was seen in globus pallidus, temporal and parietal cortex and in various thalamus nuclei. Occipital cortex showed the lowest binding among the cortex regions and white matter the lowest among all the regions studied. All the regions in case 2 (aged 67) showed higher degree of binding when compared with case 1 (aged 58), which is in agreement with previous results showing an increase in MAO-B activity with age. When the specific binding of L-[3H]deprenyl was plotted against the MAO-B activities estimated biochemically in punches from the same areas, a high positive correlation was found.
Jossan
S S
SS
Department of Pharmacology, University of Uppsala, Sweden.
Gillberg
P G
PG
d'Argy
R
R
Aquilonius
S M
SM
Långström
B
B
Halldin
C
C
Oreland
L
L
D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism; D001345:Autoradiography; D001921:Brain / Q000201:enzymology*; D002421:Caudate Nucleus / Q000187:drug effects / Q000201:enzymology; D005260:Female; D006801:Humans; D008297:Male; D008995:Monoamine Oxidase / Q000032:analysis*; D012642:Selegiline / Q000494:pharmacology*; D014055:Tomography, Emission-Computed
D012642:Selegiline; D008995:Monoamine Oxidase