A rapid and efficient method for site-directed mutagenesis by PCR, using biotinylated universal primers and streptavidin-coated magnetic beads.
Hall
L
L
Department of Biochemistry, University of Bristol, UK.
Emery
D C
DC
D001426:Bacterial Proteins; D001710:Biotin; D008280:Magnetics; D008863:Microspheres; D016297:Mutagenesis, Site-Directed; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D019809:Streptavidin
D001426:Bacterial Proteins; D015345:Oligonucleotide Probes; D001710:Biotin; D019809:Streptavidin