title: Immunogold labelling of the intermediate filament-lamina-nuclear matrix system in HeLa and BHK-21 cells.
abstract: Whole-mount, sequentially extracted cells combined with immunogold electron microscopy were developed to demonstrate the intermediate filaments, lamina, and nuclear matrix (IF-L-NM) and to identify their protein components. The IFs of HeLa cells were reacted both with keratin and vimentin monoclonal antibodies; meanwhile, the IF network of BHK-21 cell was reacted only with vimentin monoclonal antibody. The lamina and nuclear matrices of both HeLa and BHK-21 cell were labelled, respectively, with lamin monoclonal antibody-gold complex and 280 Kd nuclear matrix protein monoclonal antibody-gold complex. The monoclonal antibody to keratin could cross-react with the lamina both of HeLa and BHK-21 cells.
authors.affiliation: Department of Biology, Peking University, Beijing, People's Republic of China.
authors.forename: R J
authors.initials: RJ
authors.lastname: Jiao
authors.forename: D L
authors.initials: DL
authors.lastname: Wu
authors.forename: B
authors.initials: B
authors.lastname: Zhang
authors.forename: S T
authors.initials: ST
authors.lastname: Cai
authors.forename: Z H
authors.initials: ZH
authors.lastname: Zhai
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure*; D002478:Cells, Cultured; D006224:Cricetinae; D006046:Gold; D006367:HeLa Cells; D006801:Humans; D007382:Intermediate Filaments / Q000648:ultrastructure*; D016253:Microscopy, Immunoelectron / Q000379:methods*; D015530:Nuclear Matrix / Q000648:ultrastructure*
chemical_list: D006046:Gold