Evidence for the functional similarity between tumour cell surface guanidinobenzoatase and tissue type plasminogen activator.
Tumour cells possess a cell surface protease referred to as guanidinobenzoatase (GB). The active centre of GB binds the fluorescent probe 9-amino acridine (9-AA) and this binding enables cells possessing active GB to be located by fluorescent microscopy. GB binding of 9-AA was inhibited by prior treatment of sections of tumour tissue with a specific polyclonal antibody recognising the tumour associated protease tissue plasminogen activator (t-PA). GB binding of 9-AA was also inhibited by prior treatment of sections of tumour tissues with PAI-I, a protein inhibitor of plasminogen activatory. We conclude from these studies and kinetic analyses that GB and t-PA are very similar both in structure and function.
Steven
F S
FS
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, U.K.
Griffin
M M
MM
Cederholm-Williams
S A
SA
Mangel
W F
WF
Maier
H
H
D000585:Aminacrine / Q000378:metabolism; D001665:Binding Sites; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology; D002265:Carboxylic Ester Hydrolases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology; D002846:Chromatography, Affinity; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000473:pathology; D010450:Endopeptidases; D005260:Female; D005456:Fluorescent Dyes; D006258:Head and Neck Neoplasms / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D008856:Microscopy, Fluorescence; D010959:Tissue Plasminogen Activator / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*
D005456:Fluorescent Dyes; D000585:Aminacrine; D002265:Carboxylic Ester Hydrolases; C037288:guanidinobenzoate esterase; D010450:Endopeptidases; D010959:Tissue Plasminogen Activator