title: Anti-tumor-promotion by principles obtained from Angelica keiskei.
abstract: Potent anti-tumor promoter activity has been found in the nonpolar extracts of the root of "Ashita-Ba", Angelica keiskei Koidz. (Umbelliferae), which is eaten as a vegetable in Japan. From this active fraction, two angular furanocoumarins, archangelicin (1) and 8(S),9(R)-9-angeloyloxy-8,9-dihydrooroselol (2), three linear furanocoumarins, psoralen (3), bergapten (4) and xanthotoxin (5), and three chalcones, 4-hydroxyderricin (6), xanthoangelol (7) and a novel chalcone named ashitaba-chalcone (8), were isolated. Among these compounds, two angular type furanocoumarins, 1 and 2, and three chalcones, 6-8, suppressed 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated 32Pi-incorporation into phospholipids of cultured cells, whereas coumarins 3-5 were less effective. In addition, chalcones 6 and 7 were proved to have anti-tumor-promoting activity in mouse skin carcinogenesis induced by 7,12-dimethylbenz[a]anthracene (DMBA) plus TPA. Since chalcones 6 and 7 showed calmodulin-interacting property, both chalcones may reveal anti-tumor-promoting activity via the modulation of calmodulin involved systems. These chalcones may be useful to develop the effective method for cancer prevention.
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Meiji College of Pharmacy, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Okuyama
authors.forename: M
authors.initials: M
authors.lastname: Takata
authors.forename: J
authors.initials: J
authors.lastname: Takayasu
authors.forename: T
authors.initials: T
authors.lastname: Hasegawa
authors.forename: H
authors.initials: H
authors.lastname: Tokuda
authors.forename: A
authors.initials: A
authors.lastname: Nishino
authors.forename: H
authors.initials: H
authors.lastname: Nishino
authors.forename: A
authors.initials: A
authors.lastname: Iwashima
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification; D002147:Calmodulin / Q000494:pharmacology; D002599:Chalcone / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal; D005260:Female; D006367:HeLa Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D010743:Phospholipids / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002147:Calmodulin; D003374:Coumarins; D004365:Drugs, Chinese Herbal; D010743:Phospholipids; C068243:4-hydroxyderricin; D002599:Chalcone; C068244:xanthoangelol