Arachidonate 5-lipoxygenase of porcine pancreas: its localization in acinar cells.
Arachidonate 5-lipoxygenase has been found so far in various types of leukocyte. When a homogenate of porcine pancreas was incubated with arachidonic acid, 5-hydroxy-6,8,11,14-eicosatetraenoic acid was predominantly produced concomitant with small amounts of compounds derived from leukotriene A4. After differential centrifugation of the homogenate, the 5-lipoxygenase activity was found predominantly in the 1000 x g pellet and 105,000 x g supernatant. When porcine pancreas was investigated immunohistochemically with anti-5-lipoxygenase antibody, Langerhans islets were unstained, and infiltration of 5-lipoxygenase-positive leukocytes was hardly observed. In contrast, acinar cells were positively stained. Immunoelectron microscopy demonstrated the localization of the enzyme along the nuclear membranes of the acinar cells.
Natsui
K
K
Department of Biochemistry, Tokushima University School of Medicine, Japan.
Ueda
N
N
Yamamoto
S
S
Komatsu
N
N
Watanabe
K
K
D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D015151:Immunoblotting; D007150:Immunohistochemistry; D015289:Leukotrienes / Q000096:biosynthesis; D016253:Microscopy, Immunoelectron; D010179:Pancreas / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D013552:Swine
D015289:Leukotrienes; D001094:Arachidonate 5-Lipoxygenase