title: Control of doublesex alternative splicing by transformer and transformer-2 in Drosophila.
abstract: Sex-specific alternative processing of doublesex (dsx) precursor messenger RNA (pre-mRNA) regulates somatic sexual differentiation in Drosophila melanogaster. Cotransfection analyses in which the dsx gene and the female-specific transformer (tra) and transformer-2 (tra-2) complementary DNAs were expressed in Drosophila Kc cells revealed that female-specific splicing of the dsx transcript was positively regulated by the products of the tra and tra-2 genes. Furthermore, analyses of mutant constructs of dsx showed that a portion of the female-specific exon sequence was required for regulation of dsx pre-messenger RNA splicing.
authors.affiliation: Department of Biophysics, Faculty of Science, Kyoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hoshijima
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: I
authors.initials: I
authors.lastname: Higuchi
authors.forename: H
authors.initials: H
authors.lastname: Sakamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Shimura
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004331:Drosophila melanogaster / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes / Q000502:physiology; D005809:Genes, Regulator / Q000502:physiology; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012333:RNA, Messenger / Q000096:biosynthesis; D012091:Repetitive Sequences, Nucleic Acid; D012733:Sex Differentiation / Q000502:physiology*; D014162:Transfection
chemical_list: D012333:RNA, Messenger