[Increase of accessibility of murine lens protein fluorophores to water in the development of radiation-induced cataract].
The accessibility of tryptophanyl fluorophores in crystalline proteins to water molecules was estimated by measuring the enhancement of the fluorescence of lens homogenates in 70% D2O as compared to 100% H2O. Assuming that two sorts of fluorophores exist in the proteins, one entirely accessible to H2O and D2O and the other--absolutely not, we have calculated the portion of either group in the protein fluorescence (alpha and 1-alpha, correspondingly). Measurement of murine lens homogenates fluorescence at different stages of radiation-induced cataract, initiated with total gamma irradiation in a dose 5 Gy have shown an increased accessibility of tryptophanyl for water with cataract development. At earlier stages of cataract (appearance of scattered dot opacities) the portion of water-accessible tryptophanyl increased from 0.14 to 0.18, i.e. by a factor of 1.3. The data obtained suggest that protein globules unfold in the coarse of cataract development.
Aĭtmagambetov
M T
MT
Deev
A I
AI
Vladimirov
Iu A
IuA
D000818:Animals; D002386:Cataract / Q000209:etiology / Q000378:metabolism*; D003459:Crystallins / Q000032:analysis / Q000378:metabolism*; D005453:Fluorescence; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011829:Radiation Dosage; D011833:Radiation Injuries, Experimental; D013050:Spectrometry, Fluorescence; D014867:Water / Q000378:metabolism*
D003459:Crystallins; D014867:Water
Uvelichenie dostupnosti belkovykh fluoroforov khrustalika myshi dlia vody pri razvitii radiatsionnoĭ katarakty.