title: Identification of B2 bradykinin binding sites on cultured cortical astrocytes.
abstract: Bradykinin was found to bind to specific high-affinity sites in cultured cortical astrocytes from rat brain, and this binding appeared to be specific for the B2 bradykinin receptor subtype. Nonlinear regression analysis of saturation experiments using a computer programme revealed a single KD of 16.6 +/- 2.6 nM and a Bmax of 352.2 +/- 30.7 fmol/mg of protein. These results indicate that astrocytes possess bradykinin receptors and that these are predominantly of the B2 subtype.
authors.affiliation: Biochemistry Department, Imperial College of Science, Technology and Medicine, London, England, U.K.
authors.forename: A J
authors.initials: AJ
authors.lastname: Cholewinski
authors.forename: G
authors.initials: G
authors.lastname: Stevens
authors.forename: A M
authors.initials: AM
authors.lastname: McDermott
authors.forename: G P
authors.initials: GP
authors.lastname: Wilkin
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001665:Binding Sites; D001667:Binding, Competitive; D001920:Bradykinin / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*
chemical_list: D001920:Bradykinin