title: Autonomous growth and tumorigenicity induced by P40/interleukin 9 cDNA transfection of a mouse P40-dependent T cell line.
abstract: To test the transforming potential of deregulated P40/Interleukin 9 expression, we transfected a mouse P40-dependent T cell line with P40 cDNA, and examined the tumorigenicity of the resulting transfectants. When the cells, which grew autonomously in vitro, were injected intraperitoneally or subcutaneously into syngeneic mice, a very high tumor incidence was observed with as few as 10(4) cells per inoculum. Animals died as a result of widespread dissemination of lymphomatous tissue to abdominal and thoracic organs. The same P40-dependent cell line transfected with a control construct did not form tumors even after injection of 10(7) cells. These results indicate that uncontrolled expression of P40 can support T cell proliferation in vivo, and may be a transforming event involved in the development of certain T cell tumors.
authors.affiliation: Ludwig Institute for Cancer Research, Brussels Branch, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Uyttenhove
authors.forename: C
authors.initials: C
authors.lastname: Druez
authors.forename: J C
authors.initials: JC
authors.lastname: Renauld
authors.forename: M
authors.initials: M
authors.lastname: Hérin
authors.forename: H
authors.initials: H
authors.lastname: Noël
authors.forename: J
authors.initials: J
authors.lastname: Van Snick
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D015870:Gene Expression; D016906:Interleukin-9; D007378:Interleukins / Q000235:genetics / Q000502:physiology*; D008198:Lymph Nodes / Q000473:pathology; D051379:Mice; D009368:Neoplasm Transplantation; D013601:T-Lymphocytes; D014162:Transfection
chemical_list: D016906:Interleukin-9; D007378:Interleukins