title: pH dependence of 5-fluorouracil uptake observed by in vivo 31P and 19F nuclear magnetic resonance spectroscopy.
abstract: Multinuclear nuclear magnetic resonance spectroscopy was used to follow the metabolism and kinetics of 5-fluorouracil (5FU) after i.v. administration at a dose of 100 mg/kg on Wistar rats. 31P spectra allow one to determine both the energetic status and the pH of the tissues under investigation, while serial 19F spectra reveal the drug clearance. Analyses of 5FU kinetics show that the half-life of 5FU elimination is about 35 min in tissue with a pH of 7.3. However, this half-life increases 2.5-fold when the local pH decreases below 6.9. Thus, acidification seems to induce a local retention of 5FU, which tends to prove the existence of active transport. This retention of the drug may have significant clinical implications for assessing and improving chemotherapy alone or in combination.
authors.affiliation: Institut Curie-Biologie, INSERM U 219, Orsay, France.
authors.forename: J L
authors.initials: JL
authors.lastname: Guerquin-Kern
authors.forename: F
authors.initials: F
authors.lastname: Leteurtre
authors.forename: A
authors.initials: A
authors.lastname: Croisy
authors.forename: J M
authors.initials: JM
authors.lastname: Lhoste
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D005354:Fibrosarcoma / Q000139:chemically induced / Q000378:metabolism*; D005461:Fluorine / Q000378:metabolism; D005472:Fluorouracil / Q000008:administration & dosage / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D010758:Phosphorus / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014508:Urea / Q000031:analogs & derivatives / Q000378:metabolism; D015091:beta-Alanine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D015091:beta-Alanine; D010758:Phosphorus; D005461:Fluorine; C032348:alpha-fluoro-beta-alanine; C045635:alpha-fluoro-beta-ureidopropionic acid; D015127:9,10-Dimethyl-1,2-benzanthracene; D014508:Urea; D005472:Fluorouracil