Murine recombinant leukemia inhibitory factor modulates inhibitory effect of 1,25 dihydroxyvitamin D3 on alkaline phosphatase activity in MC3T3-E1 cells.
We demonstrated murine leukemia inhibitory factor (mLIF) mRNA in osteoblastic MC3T3-E1 cells, but not mLIF in their conditioned medium. Recombinant mLIF had an inhibitory effect on alkaline phosphatase (ALP) activity, but not on DNA synthesis, in these mLIF-free cells. This inhibitory effect was not prostaglandin E2 dependent. mLIF also modulated the inhibitory effect of 1,25 dihydroxyvitamin D3 [1,25(OH)2D3] on ALP activity, partly via down regulation of 1,25(OH)2D3 binding sites. These results suggest that LIF may play a role in regulating osteoblast differentiation.
Hakeda
Y
Y
Department of Oral Anatomy, Meikai University School of Dentistry, Saitama, Japan.
Sudo
T
T
Ishizuka
S
S
Tanaka
K
K
Higashino
K
K
Kusuda
M
M
Kodama
H
H
Kumegawa
M
M
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D000818:Animals; D015152:Blotting, Northern; D002117:Calcitriol / Q000494:pharmacology*; D002460:Cell Line; D003001:Cloning, Molecular; D015870:Gene Expression; D006131:Growth Inhibitors; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins
D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000469:Alkaline Phosphatase; D002117:Calcitriol; D007213:Indomethacin