Subcellular distribution of adenylyl cyclase and Gs alpha in rat brown adipose tissue.
The subcellular distribution of Gs alpha (the alpha-subunit of guanine nucleotide-binding stimulatory protein of adenylyl cyclase) was examined in interscapular brown adipose tissue (IBAT) to determine (1) if Gs alpha is completely colocalized with adenylyl cyclase in the plasma membrane, and (2) whether cold exposure, which increases adenylyl cyclase activity, changes the subcellular distribution of Gs alpha. Subcellular fractions were prepared from IBAT by differential centrifugation and analyzed for Gs alpha by immunoblotting. Adenylyl cyclase activity and Gs alpha were detected in all the subcellular fractions except the cytosol. The plasma membrane fraction showed the greatest enrichment of adenylyl cyclase and Gs alpha. However, the enrichment of adenylyl cyclase in the plasma membrane fraction was greater than that for Gs alpha, which was also associated to a large degree with the mitochondrial fraction. Thus, compared with the mitochondrial fraction, both 5' nucleotidase and adenylyl cyclase were enriched by over 200% in the plasma membrane fraction, but Gs alpha was enriched by only 50%. Exposure of rats to 4 degrees C for 3 days increased fluoride-stimulated adenylyl cyclase activity, but did not increase the amount of immunoreactive Gs alpha in any of the subcellular fractions examined. The above results demonstrate that not all Gs alpha in IBAT is colocalized with adenylyl cyclase in the plasma membrane. The finding that cold exposure did not change the subcellular distribution of Gs alpha indicates that the cold-induced increase in adenylyl cyclase activity is not due to translocation of Gs alpha from subcellular compartments to the plasma membrane.
Chaudhry
A
A
Center for Cell Biology, Sinai Research Institute, Detroit, MI 48235.
Granneman
J G
JG
D015720:5'-Nucleotidase / Q000378:metabolism; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism*; D002001:Adipose Tissue, Brown / Q000378:metabolism / Q000648:ultrastructure*; D000818:Animals; D002458:Cell Fractionation; D002462:Cell Membrane / Q000378:metabolism; D002772:Cholera Toxin / Q000378:metabolism; D003080:Cold Temperature; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000378:metabolism*
D000246:Adenosine Diphosphate Ribose; D002772:Cholera Toxin; D015720:5'-Nucleotidase; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases