The expression of actin mRNA in megakaryocytes demonstrated in paraffin sections, and epoxy resin semi-thin and thin sections by in situ hybridization.
Megakaryocytes of dog bone marrow were utilized as target cells for identifying actin mRNA expressing cells on semi-thin and thin sections. After in situ hybridization with radioisotope-labeled probes was performed on paraffin sections, gelatin capsules containing freshly prepared epoxy resin were placed on the sections. The resin was solidified and detached from the slide glass, and semi-thin and thin sections were obtained. The signals showing actin mRNA expression were detected on megakaryocytes in these sections by light and electron microscopy.
Sasaki
J
J
Department of Anatomy, Okayama University Medical School, Japan.
Nomura
T
T
Watanabe
S
S
Kanda
S
S
Watanabe
H
H
Miake
K
K
Noji
S
S
Taniguchi
S
S
D000199:Actins / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D001853:Bone Marrow / Q000378:metabolism / Q000648:ultrastructure; D001854:Bone Marrow Cells; D004285:Dogs; D015870:Gene Expression; D008533:Megakaryocytes / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism
D000199:Actins; D015347:RNA Probes; D012333:RNA, Messenger