Recombinant DNA approaches to study the role of the regulatory light chains (RLC) using scallop myosin as a test system.
The ability to exchange reversibly the regulatory light chains (RLCs) from scallop myosin has provided us with a test system to probe the mechanisms of regulation mediated by the RLCs from vertebrate skeletal, vertebrate smooth and molluscan myosins. The cloning and expression of these RLCs, together with domain-swapping and site-directed mutagenesis approaches, has allowed us to explore further the mechanisms involved and identify the functional importance of specific regions of the RLC molecule; for example, the presence of a high affinity metal binding site in the N-terminal domain and its interaction with the intact C-terminal domains are required for regulation.
Kendrick-Jones
J
J
MRC Laboratory of Molecular Biology, Cambridge, UK.
Rasera da Silva
A C
AC
Reinach
F C
FC
Messer
N
N
Rowe
T
T
McLaughlin
P
P
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000235:genetics*; D008969:Molecular Sequence Data; D008974:Mollusca; D009218:Myosins / Q000235:genetics*
D004274:DNA, Recombinant; D009218:Myosins