Chemical properties and anti-complementary activities of heteroglycans from the leaves of Panax ginseng.
Four anti-complementary neutral (GL-NIa and GL-NIb) and acidic (GL-AIa and GL-AIb) polysaccharides were purified from the leaves of Panax ginseng C. A. Meyer. Only GL-NIa and GL-AIa exhibited potent anti-complementary activities at low concentrations probably through the alternative complement pathway. Glycosyl linkage analysis demonstrated that GL-NIa mainly consisted of arabinogalactan moieties, whereas GL-NIb contained large amounts of (1----4)-linked glucosyl residues. Beta-Elimination indicated that GL-AIa and GL-AIb were pectic polysaccharides consisting of a rhamnogalacturonan core with neutral side chains. GL-AIa reacted strongly with beta-glucosyl-Yariv antigen, GL-NIa reacted weakly, whereas GL-NIb and GL-AIb showed no reaction with the antigen.
Gao
Q P
QP
Oriental Medicine Research Center, Kitasato Institute, Tokyo, Japan.
Kiyohara
H
H
Cyong
J C
JC
Yamada
H
H
D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D003169:Complement Inactivator Proteins / Q000737:chemistry / Q000302:isolation & purification; D008969:Molecular Sequence Data; D005894:Panax / Q000032:analysis*; D010946:Plants, Medicinal; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D002241:Carbohydrates; D003169:Complement Inactivator Proteins; D011134:Polysaccharides