The role of the SOS response in bacteria exposed to zidovudine or trimethoprim.
Trimethoprim was more potent than zidovudine as an inducer of the SOS response in Escherichia coli. The level of induction by each compound initially increased with rising drug concentration and then fell; this effect was less marked with zidovudine than with trimethoprim. The SOS response did not appear to be involved in the inhibition of bacterial multiplication as the MICs of trimethoprim or zidovudine for recA430 and lexA3 mutants, which are unable to induce the SOS response, were identical to the MICs for the parent strains. However, the bactericidal activity of each compound against strains deficient in the SOS response was reduced. This suggest that induction of the DNA repair system contributes to the bactericidal activity of the drugs.
Lewin
C S
CS
Department of Bacteriology, University of Edinburgh Medical School.
Amyes
S G
SG
D002470:Cell Survival / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli; D013014:SOS Response, Genetics / Q000187:drug effects*; D013936:Thymidine / Q000494:pharmacology; D014295:Trimethoprim / Q000494:pharmacology*; D015215:Zidovudine / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000096:biosynthesis
D015215:Zidovudine; D014295:Trimethoprim; D001616:beta-Galactosidase; D013936:Thymidine