DNA replication of wheat dwarf virus, a geminivirus, requires two cis-acting signals.
Replication of the single-stranded DNA genome of wheat dwarf virus (WDV) leads to the accumulation of covalently closed double-stranded DNA of genome length in infected cells. By studying the replication properties of a naturally occurring deletion mutant of WDV isolated from infected plants and of deletion mutants constructed in vitro, we have defined cis-acting regions required for viral DNA replication. The results show that two distinct regions are required in cis to yield the normal replicative forms of WDV-DNA.
Kammann
M
M
Max-Planck-Institut für Züchtungsforschung, Köln, FRG.
Schalk
H J
HJ
Matzeit
V
V
Schaefer
S
S
Schell
J
J
Gronenborn
B
B
D015139:Blotting, Southern; D002872:Chromosome Deletion; D004252:DNA Mutational Analysis; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics; D010942:Plant Viruses / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014908:Triticum; D014779:Virus Replication
D004279:DNA, Viral