Characterization of the opposite-strand genes from the mouse bidirectionally transcribed HTF9 locus.
The mouse HTF9 locus contains two genes that are bidirectionally transcribed with opposite polarity from a shared CpG-rich island. Both genes were previously shown to be expressed in a housekeeping fashion in mouse. We have now determined the molecular organization of the genes over 12 kb surrounding the island. In addition, we show that the HTF9 locus resides in the proximal region of mouse chromosome 16. We have sequenced the cDNAs corresponding to both divergent transcripts. Both genes appear to code for novel proteins that are structurally unrelated to each other. Finally, we show that both genes are highly conserved and efficiently expressed in human cells.
Bressan
A
A
Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, Rome, Italy.
Somma
M P
MP
Lewis
J
J
Santolamazza
C
C
Copeland
N G
NG
Gilbert
D J
DJ
Jenkins
N A
NA
Lavia
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003433:Crosses, Genetic; D005091:Exons / Q000235:genetics; D015870:Gene Expression / Q000502:physiology; D005807:Genes, Overlapping / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D007438:Introns / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011506:Proteins / Q000235:genetics; D016601:RNA-Binding Proteins; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D020931:ran GTP-Binding Protein
D009687:Nuclear Proteins; D011506:Proteins; D016601:RNA-Binding Proteins; C091775:ran-binding protein 1; C070847:Htf9c protein, mouse; D020931:ran GTP-Binding Protein