title: Cocontamination of swine diets by aflatoxin and diacetoxyscirpenol.
abstract: The effects of dietary aflatoxin (AF) and diacetoxyscirpenol (DAS), singly and in combination, were evaluated in growing crossbred barrows. The experimental design consisted of 4 treatments of 9 barrows each fed diets containing 1) 0 mg AF and 0 mg DAS/kg feed (control), 2) 2.5 mg AF/kg feed, 3) 2.0 mg DAS/kg feed, or 4) 2.5 mg AF + 2.0 mg DAS/kg feed for 28 days (10-14 weeks of age). Production performance, serum biochemical, hematologic, and pathologic measurements were made. Body weight and body weight gain were significantly decreased by each toxin but more so by the combination treatment. The effects were additive in nature. Liver and spleen weights, as percentages of body weight, were increased by the AF and AF + DAS treatments, and AF or AF + DAS treatments induced diffuse hepatocellular vacuolar change, early portal fibrosis, and early bile duct hyperplasia. Aflatoxin increased serum values of creatinine and gamma glutamyl transferase, cholinesterase, and alkaline phosphatase activities; increased packed cell volume and hemoglobin; and decreased urea nitrogen and total iron binding capacity. DAS reduced serum iron binding capacity. The AF + DAS treatment increased serum gamma glutamyl transferase and alkaline phosphatase activities, increased hemoglobin, and decreased serum iron binding capacity. Generally, the combination treatment could be described as additive or less than additive, with most of the effects attributable to AF. Under the conditions and parameters monitored in this study, AF and DAS had no synergistic toxic effects when incorporated into diets of growing barrows.
authors.affiliation: Food Animal Protection Research Laboratory, US Department of Agriculture, College Station, TX 77840.
authors.forename: R B
authors.initials: RB
authors.lastname: Harvey
authors.forename: L F
authors.initials: LF
authors.lastname: Kubena
authors.forename: M H
authors.initials: MH
authors.lastname: Elissalde
authors.forename: D E
authors.initials: DE
authors.lastname: Corrier
authors.forename: W E
authors.initials: WE
authors.lastname: Huff
authors.forename: G E
authors.initials: GE
authors.lastname: Rottinghaus
authors.forename: B A
authors.initials: BA
authors.lastname: Clement
mesh_terms: D000348:Aflatoxins / Q000633:toxicity*; D000818:Animals; D004435:Eating / Q000187:drug effects; D005506:Food Contamination; D008099:Liver / Q000187:drug effects; D008297:Male; D009183:Mycotoxins / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D011897:Random Allocation; D013154:Spleen / Q000187:drug effects; D013552:Swine; D013553:Swine Diseases / Q000139:chemically induced*; D014255:Trichothecenes / Q000633:toxicity*; D015430:Weight Gain / Q000187:drug effects
chemical_list: D000348:Aflatoxins; D009183:Mycotoxins; D014255:Trichothecenes; C001809:diacetoxyscirpenol