Quantitative determination of aflatoxin B1 in chick liver.
A method for the rapid and quantitative determination of aflatoxin B1 from small quantities of liver, around 1-2 g, is described. The extraction procedure involves acidification to pH 2 of the aqueous liver homogenates, extraction with chloroform: acetone and HPLC-fluorimetric detection after derivatization with trifluoroacetic acid. Quantitative recovery of aflatoxin B1 from chick liver was achieved and detection at levels of 0.2-1 ppb was proved feasible. The aflatoxin B1 concentration in chick liver after oral administration is also shown.
Espada
Y
Y
Department of Veterinary Medicine, Faculty of Veterinary, Autonomous University of Barcelona, Bellaterra, Spain.
Guitart
R
R
Arboix
M
M
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D002138:Calibration; D002273:Carcinogens / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D008099:Liver / Q000737:chemistry*; D015203:Reproducibility of Results; D014269:Trifluoroacetic Acid
D000348:Aflatoxins; D002273:Carcinogens; D016604:Aflatoxin B1; D014269:Trifluoroacetic Acid