[Quantitative imaging of the heterogeneity of membrane activation of mammalian neurons and glial cells].
By using quantitative imaging with an ultra-high sensitivity, it was possible to observe the simultaneous action of multiple patches unevenly distributed over the membranes of neurons and glial cells in culture. We used a voltage-sensitive probe to stain vitally the cells. The instrumentation consisted of a liquid-nitrogen cooled matrix of 222,530 photodetectors with a spatial resolution of 0.25 microns 2, a photodynamic range of 10(5), a detection level of a few tens of photons and a maximum time resolution of 500 microseconds. Electrical and pharmacological stimulations were applied to produce the activation of the cells which was accompanied by large variations of the level of fluorescence, giving a precise spatial localization of active domains over the soma-neuritic membranes. These images of fluorescent signals are interpreted as corresponding to the plasmalemmal localization of voltage-dependent channels. This finding, which had not been previously observed with voltage-sensitive probes in fluorescent dye imaging indicates the possibility of measuring the activity of independently functioning domains in single neurons.
Gogan
P
P
Unité de Neurocybernétique cellulaire, C.N.R.S.-U.P.R. 418 et I.N.S.E.R.M. no. U6, Marseille.
Ternaux
J P
JP
Tyc-Dumont
S
S
D000818:Animals; D002462:Cell Membrane / Q000502:physiology*; D005724:Ganglia / Q000166:cytology*; D008856:Microscopy, Fluorescence / Q000379:methods; D009474:Neurons / Q000648:ultrastructure*; D055095:Optics and Photonics; D010783:Photometry / Q000379:methods; D051381:Rats
Visualisation quantitative de l'hétérogénéité de l'activation membranaire des neurones et de la glie de mammifère.