Effect of an adjacent base on detection of a point mutation by restriction enzyme digestion.
While routinely mapping point mutations within the arginase locus of a collection of hyperargininemic patients, we discovered that a base immediately outside a restriction endonuclease recognition site (TaqI) can eliminate cleavage of this site by this enzyme. The genetic lesion lay in a base immediately flanking a TaqI recognition site within exon 8 of the arginase locus and abolished cutting by approximately 80%. We wish to emphasize the necessity of heeding subtle cues frequently encountered while generating restriction enzyme data, because neither Southern blot maps nor endonuclease digestion of polymerase chain reaction amplified products of exon 8 accurately predicted where the point mutation lay. To our knowledge, this is the first instance of inhibition of cleavage by flanking bases occurring on natural (nonsynthetic) DNA substrates, i.e., within the clinical setting of characterization of a human genetic disorder.
Klein
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D
Department of Psychiatry, UCLA School of Medicine, Los Angeles, California 90024.
Dodson
A E
AE
Tabor
D E
DE
Cederbaum
S D
SD
Grody
W W
WW
D001483:Base Sequence; D015139:Blotting, Southern; D003720:Densitometry; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D006868:Hydrolysis; D008661:Metabolism, Inborn Errors / Q000175:diagnosis / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases