title: 13C NMR spectroscopic analysis of phospholipid metabolism in adrenal chromaffin cells.
abstract: Phosphatidylcholine (PC) metabolism in bovine adrenal chromaffin cells has been studied in vivo with 100 MHz 13C NMR spectroscopy. The incorporation of 13[CH3]choline was examined in cells embedded in 1% agarose strands and perfused at 37 degrees C. The pattern of 13C NMR spectroscopy. The incorporation of [13CH3]choline incorporation into PC of suspension cultures of chromaffin cells. The chemical shifts of labelled carbons in [13CH3]choline before and after their incorporation into PC are identical (ca 56.4 ppm). Incorporated [13CH3]choline can be released from the cells by phospholipase D which reduces the cellular PC signal by greater than 85%, indicating that a major fraction of the labelled PC is on the external membrane surface. This application of NMR spectroscopy provides a basis for direct measurement of the in vivo metabolism of PC in functioning adrenal chromaffin cells.
authors.affiliation: Program of Neurosciences, Baylor College of Medicine, Houston, TX 77030.
authors.forename: C J
authors.initials: CJ
authors.lastname: Rivera
authors.forename: G A
authors.initials: GA
authors.lastname: Plishker
authors.forename: J D
authors.initials: JD
authors.lastname: Morrisett
authors.forename: J
authors.initials: J
authors.lastname: Moore
authors.forename: A K
authors.initials: AK
authors.lastname: Percy
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002794:Choline / Q000378:metabolism; D002837:Chromaffin Granules / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010713:Phosphatidylcholines / Q000378:metabolism*
chemical_list: D010713:Phosphatidylcholines; D002794:Choline