Detection of HTLV-1 gag related sequences in leucocyte DNA from patients with polyendocrinopathies (Basedow-Graves' disease and insulin-dependent diabetes).
Relationships with retroviruses have recently been found in different human pathologies as autoimmune diseases which would be associated with the presence and eventually the expression of retroviral sequences. Detection of the presence of HTLV-1 and HIV-1 homologous sequences and their expression was realised on lymphocytes of 14 patients with polyendrocrinopathies (Basedow-Graves' disease and insulin-dependent diabetes) and four relatives of one index case. No antibodies to HTLV-1 and HIV-1 could be detected by Western blot and Elisa tests. HTLV-1 related sequences were revealed by Southern blot (SB) in 5 out of 18 subjects' DNA. Analyses of all DNA were performed by polymerase chain reaction (PCR). Seven DNA, including the 5 previously positive in SB, and two relatives (father and grandfather), negative in SB, contained HTLV-1-gag related sequences, but neither pol nor pX regions. Concerning HIV-1, all 18 DNA examined were negative by both methods. DNA of ten clinically healthy donors were found to be negative with the same tests.
Lagaye
S
S
U.P.R. A0043, C.N.R.S. Rétrovirus et Rétrotransposons des Vertébrés, Hôpital Saint-Louis, Paris.
Vexiau
P
P
Morozov
V
V
Saal
F
F
Gony
J
J
Poorters
A M
AM
Loosfeld-Poirot
Y
Y
Hors
J
J
Cathelineau
G
G
Emanoil-Ravier
R
R
D000368:Aged; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000737:chemistry*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000150:complications*; D015750:Genes, gag / Q000235:genetics*; D006111:Graves Disease / Q000097:blood / Q000150:complications*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D007962:Leukocytes; D008875:Middle Aged; D008969:Molecular Sequence Data
D004247:DNA