Development of a luminescence immunoassay for follitropin suitable for clinical routine.
We developed a luminescence immunoassay (LIA) for follitropin, based on the synthesis of a follitropin-N-(4-aminobutyl)-N-ethylisoluminol conjugate. The luminescence tracer was purified by gel chromatography. Antibody-bound and non-bound tracer fractions were separated by using a second antibody reagent bound to magnetic particles. The assay can be performed within 24 hours and is sufficiently sensitive for the measurement of all clinically relevant follitropin concentrations including the subnormal range.
Biro
G
G
Medizinische Klinik und Poliklinik, Innere Medizin II, Universität des Saarlandes, Germany.
Samira
A
A
Butz
H
H
Leicht
E
E
Weinges
K F
KF
D000704:Analysis of Variance; D003955:Diagnostic Tests, Routine / Q000379:methods; D005640:Follicle Stimulating Hormone / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007202:Indicators and Reagents; D008163:Luminescent Measurements
D007202:Indicators and Reagents; D005640:Follicle Stimulating Hormone