Activation of human T lymphocytes by Leishmania lipophosphoglycan.
This study describes Leishmania antigen-induced activation of lymphocytes isolated from Kenyan donors, previously treated for visceral leishmaniasis, and from Danish and Kenyan controls. Peripheral blood mononuclear cells (PBMC) from cured Kala-Azar patients proliferated and produced Interferon-gamma in vitro in response to lipophosphoglycan (LPG) isolated from Leishmania major. The proliferative response was mainly due to activation of CD2-positive T cells. PBMC from controls did not respond to LPG, but to sonicates prepared from both L. major and L. donovani promastigotes. The surface glycoprotein GP 63 failed to activate PBMC from any of the donors tested. These results show that the individuals cured from visceral leishmaniasis had expanded T-cell clones recognizing LPG, conceivably as a result of Leishmania infection. The LPG preparation was without detectable protein contamination. Thus, the results suggest that human T lymphocytes can respond to glycolipid antigens.
Kemp
M
M
Department of Infectious Diseases, University Hospital, University of Copenhagen, Denmark.
Theander
T G
TG
Handman
E
E
Hey
A S
AS
Kurtzhals
J A
JA
Hviid
L
L
Sørensen
A L
AL
Were
J O
JO
Koech
D K
DK
Kharazmi
A
A
D000818:Animals; D000953:Antigens, Protozoan / Q000494:pharmacology; D000969:Antinematodal Agents / Q000627:therapeutic use; D006028:Glycosphingolipids / Q000494:pharmacology*; D006801:Humans; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000378:metabolism; D007895:Leishmania tropica / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000188:drug therapy / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D008666:Metalloendopeptidases; D015800:Protozoan Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*
D000953:Antigens, Protozoan; D000969:Antinematodal Agents; D006028:Glycosphingolipids; D015800:Protozoan Proteins; C008290:lipophosphonoglycan; D007371:Interferon-gamma; D008666:Metalloendopeptidases; C055146:glycoprotein gp63, Leishmania