title: Normal unstimulated lymphocytes produce granulopoietic inhibitory activity.
abstract: Normal unstimulated lymphocytes cocultured with normal bone marrow will inhibit day-7 colony- forming units in culture. We have shown that this phenomenon has a molecular basis and attempted to characterize it further. Using specific assays and neutralizing monoclonal antibodies we have found that this granulopoietic inhibitory activity (GIA) is not due to alpha- or gamma-interferon, tumor necrosis factor, or acidic or basic isoferritins. Biochemical studies suggest that it is a glycoprotein with a molecular weight greater than 100,000 daltons. It appears to act on cells in S phase, although it may not be S-phase specific. GIA represents a novel inhibitor that merits further investigation.
authors.affiliation: Department of Haematology, Queen's University of Belfast, Northern Ireland.
authors.forename: A E
authors.initials: AE
authors.lastname: Irvine
authors.forename: M A
authors.initials: MA
authors.lastname: French
authors.forename: J M
authors.initials: JM
authors.lastname: Bridges
authors.forename: A D
authors.initials: AD
authors.lastname: Crockard
authors.forename: Z R
authors.initials: ZR
authors.lastname: Desai
authors.forename: T C
authors.initials: TC
authors.lastname: Morris
mesh_terms: D001853:Bone Marrow / Q000502:physiology; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D006023:Glycoproteins / Q000378:metabolism / Q000502:physiology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000037:antagonists & inhibitors; D006098:Granulocytes / Q000502:physiology*; D006410:Hematopoiesis / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007370:Interferon Type I / Q000502:physiology; D007371:Interferon-gamma / Q000502:physiology; D008213:Lymphocyte Activation / Q000502:physiology; D008214:Lymphocytes / Q000378:metabolism / Q000502:physiology*; D016196:S Phase / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
chemical_list: D006023:Glycoproteins; D007370:Interferon Type I; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor