Macrophage NO2- production as a sensitive and rapid assay for the quantitation of murine IFN-gamma.
Macrophage production of arginine-derived NO2- provides a simple method for detection and quantitation of interferon-gamma (IFN-gamma) in murine cell culture fluids. When the macrophage cell line RAW 264 is cultured overnight with IFN-gamma in the presence of 10 ng/ml LPS, NO2- release, as determined by a simple colorimetric assay, is proportional to the concentration of IFN-gamma and is inhibited by monoclonal antibody to IFN-gamma. A high degree of correlation was obtained when antigen stimulated T lymphocyte supernatants were tested for IFN-gamma by ELISA and the NO2- production assay.
Migliorini
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P
Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
Corradin
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G
Corradin
S B
SB
D000818:Animals; D001681:Biological Assay; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D007371:Interferon-gamma / Q000032:analysis* / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009573:Nitrites / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D008070:Lipopolysaccharides; D009573:Nitrites; D016212:Transforming Growth Factor beta; D007371:Interferon-gamma