L-tyrosine induces tyrosinase expression via a posttranscriptional mechanism.
Exposure of hamster amelanotic melanoma cells to L-tyrosine caused a time-dependent increase of tyrosinase protein concentrations, tyrosinase activity and level of cell pigmentation. In contrast, Northern blot analysis using mouse tyrosinase cDNA showed a steady level of tyrosinase mRNA. Thus in hamster melanoma cells the stimulation of intracellular tyrosinase concentration by L-tyrosine is mediated mainly via a posttranscriptional mechanism.
Slominski
A
A
Department of Immunology and Microbiology, Albany Medical College, New York 12208.
Costantino
R
R
D000818:Animals; D008543:Melanins / Q000378:metabolism*; D008545:Melanoma; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000494:pharmacology*
D008543:Melanins; D012333:RNA, Messenger; D014443:Tyrosine; D014442:Monophenol Monooxygenase