title: High-sensitivity laser-induced fluorescence detection for capillary electrophoresis.
abstract: Capillary electrophoresis provides very high efficiency separations of biological molecules. Laser-induced fluorescence produces very high sensitivity detection. The combination of the two techniques results in an analytical tool with unprecedented properties: separations with more than a million theoretical plates and detection limits of a few hundred analyte molecules. This paper considers the design of high-sensitivity laser-induced fluorescence detection for capillary zone electrophoresis separation of labeled amino acids and capillary gel electrophoresis separation of DNA sequencing fragments.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Canada.
authors.forename: J Z
authors.initials: JZ
authors.lastname: Zhang
authors.forename: D Y
authors.initials: DY
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: Wu
authors.forename: H R
authors.initials: HR
authors.lastname: Harke
authors.forename: N J
authors.initials: NJ
authors.lastname: Dovichi
mesh_terms: D000596:Amino Acids / Q000032:analysis; D004247:DNA / Q000032:analysis; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D007834:Lasers; D009696:Nucleic Acids / Q000032:analysis; D013050:Spectrometry, Fluorescence
chemical_list: D000596:Amino Acids; D009696:Nucleic Acids; D004247:DNA