Toxicity of cyst extract of Sarcocystis fusiformis from buffalo in rabbits and mice.
The toxic effect of cysts of Sarcocystis fusiformis (of buffalo origin) in rabbits and mice have been studied. It is shown that inoculation of a 2 mg ml-1 concentration of protein of the macrosarcocyst extract (ME) into rabbits and mice was lethal. The toxic substance was thermolabile at greater than or equal to 60 degrees C for 30 min. Protease and trypsin caused inactivation, while papain did not affect the toxicity. ME was found to be non-dialysable, but precipitable. There was also no effect of a pH of 6-9, while pH 4-5 altered the toxicity and pH 10 only partially affected the toxic effect.
Saleque
A
A
Department of Parasitology, College of Veterinary Sciences, G.P. Pant University of Agriculture and Technology, India.
Bhatia
B B
BB
Juyal
P D
PD
Rahman
H
H
D000818:Animals; D002020:Buffaloes / Q000469:parasitology*; D010450:Endopeptidases / Q000494:pharmacology; D005260:Female; D006358:Hot Temperature; D008297:Male; D051379:Mice; D010206:Papain / Q000494:pharmacology; D015800:Protozoan Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000633:toxicity*; D011817:Rabbits; D012522:Sarcocystis / Q000502:physiology*; D012523:Sarcocystosis / Q000469:parasitology / Q000662:veterinary*; D014118:Toxins, Biological / Q000737:chemistry / Q000633:toxicity*; D014357:Trypsin / Q000494:pharmacology
D015800:Protozoan Proteins; D014118:Toxins, Biological; D010450:Endopeptidases; D014357:Trypsin; D010206:Papain