Biological sulfane sulfur.
A voltammetric method for determining cyanide-reactive sulfane sulfur in biological materials is described. Samples are incubated with a sulfurtransferase, a thiolic cofactor, and cyanide. Thiocyanate formed and/or residual cyanide may then be determined electrochemically with either a silver rotating disk electrode or a dropping mercury electrode in differential pulse mode to provide estimates of sulfane sulfur content. The thiocyanate-based procedure is preferable, particularly when samples contain either serum albumin or inorganic sulfide.
Westley
A M
AM
Department of Biochemistry, University of Chicago, Illinois 60637.
Westley
J
J
D000818:Animals; D002417:Cattle; D003486:Cyanides / Q000032:analysis; D006801:Humans; D012044:Regression Analysis; D013455:Sulfur / Q000032:analysis*; D013861:Thiocyanates / Q000032:analysis
D003486:Cyanides; D013861:Thiocyanates; D013455:Sulfur