Effects of antisurfactant antibodies on the course of mild respiratory distress syndrome.
The role of surfactant-associated proteins in surfactant function was studied by selectively blocking these proteins with monoclonal antibodies. Four monoclonal antibodies, M1, M2, M3, and M4 were identified and their reactivities examined by Western blot analysis. M1, M2, and M4 bind, respectively, 8-, 10- and both 10- and 34-kD proteins. M3 antibody did not recognize a protein as assayed by this technique. These antibodies were then administered intratracheally to adult rats that had been partially depleted of lung surfactant by broncholavage. None of these antibodies had any deleterious effect on pulmonary function. On the other hand, M4 antibody significantly improved gas exchange. Possible mechanisms by which antibody may effect such improvement are discussed.
Eijking
E P
EP
Department of Anesthesiology, Erasmus University, Rotterdam, The Netherlands.
Strayer
D S
DS
van Daal
G J
GJ
Lachmann
B
B
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015153:Blotting, Western; D002245:Carbon Dioxide / Q000097:blood; D010100:Oxygen / Q000097:blood; D011659:Pulmonary Gas Exchange; D011663:Pulmonary Surfactants / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012128:Respiratory Distress Syndrome / Q000276:immunology / Q000473:pathology*
D000911:Antibodies, Monoclonal; D011663:Pulmonary Surfactants; D002245:Carbon Dioxide; D010100:Oxygen