title: Construction of a family of lactococcal vectors for gene cloning and translational fusion.
abstract: A family of stable lactococcal vectors have been constructed based on the pFX1 replicon using either the alpha fragment or the complete Escherichia coli lacZ gene as a selective marker. These vectors also incorporate multiple cloning sites and examples are given of their use for gene cloning and translational fusion studies in lactococci.
authors.affiliation: Department of Biotechnology, Massey University, Palmerston North, New Zealand.
authors.forename: F F
authors.initials: FF
authors.lastname: Xu
authors.forename: L E
authors.initials: LE
authors.lastname: Pearce
authors.forename: P L
authors.initials: PL
authors.lastname: Yu
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005822:Genetic Vectors; D007763:Lac Operon; D013294:Lactococcus lactis / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D014176:Protein Biosynthesis; D012093:Replicon; D015183:Restriction Mapping