Autonomous growth and tumorigenicity induced by P40/interleukin 9 cDNA transfection of a mouse P40-dependent T cell line.
To test the transforming potential of deregulated P40/Interleukin 9 expression, we transfected a mouse P40-dependent T cell line with P40 cDNA, and examined the tumorigenicity of the resulting transfectants. When the cells, which grew autonomously in vitro, were injected intraperitoneally or subcutaneously into syngeneic mice, a very high tumor incidence was observed with as few as 10(4) cells per inoculum. Animals died as a result of widespread dissemination of lymphomatous tissue to abdominal and thoracic organs. The same P40-dependent cell line transfected with a control construct did not form tumors even after injection of 10(7) cells. These results indicate that uncontrolled expression of P40 can support T cell proliferation in vivo, and may be a transforming event involved in the development of certain T cell tumors.
Uyttenhove
C
C
Ludwig Institute for Cancer Research, Brussels Branch, Belgium.
Druez
C
C
Renauld
J C
JC
Hérin
M
M
Noël
H
H
Van Snick
J
J
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D015870:Gene Expression; D016906:Interleukin-9; D007378:Interleukins / Q000235:genetics / Q000502:physiology*; D008198:Lymph Nodes / Q000473:pathology; D051379:Mice; D009368:Neoplasm Transplantation; D013601:T-Lymphocytes; D014162:Transfection
D016906:Interleukin-9; D007378:Interleukins