title: An immunocytochemical analysis of a class-specific antibody response against Trichinella spiralis in humans.
abstract: The response of different classes of antibodies against antigens of the muscle larvae of Trichinella spiralis was tested using an immunocytochemical approach. Ultrathin sections of resin-embedded larvae were treated with sera from patients with trichinosis, then exposed to a biotinated second antibody and stained with avidin-gold complex. Antibody of the M-class was a major component in the response against a slow-responding group of antigens that included stichocyte granules, the cuticle surface, and the esophagus-occupying substance; a minor component in the response against antigens of the rapid-responding group that included cuticle inner layers, hypodermis, hemolymph, and intestinal gland granules. The response of G-class antibody against the rapid-responding group of antigens was detected in all patients tested, while against the slow-responding group of antigens it was detected in only half of the patients, suggesting that an antibody shift from the M to the G class occurred in some patients. The results, obtained in humans, were similar to those we obtained previously in rats (J. Parasitology, 76,230-239, 1990), suggesting that the rat immune system can serve as an experimental model of human trichinosis.
authors.affiliation: Department of Parasitology, Nara Medical University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Uno
authors.forename: N
authors.initials: N
authors.lastname: Mizuno
authors.forename: C
authors.initials: C
authors.lastname: Tokuda
authors.forename: K
authors.initials: K
authors.lastname: Shimazu
authors.forename: T
authors.initials: T
authors.lastname: Araki
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology* / Q000378:metabolism; D000917:Antibody Formation / Q000502:physiology*; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000276:immunology; D006046:Gold; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D007075:Immunoglobulin M / Q000276:immunology / Q000378:metabolism; D007150:Immunohistochemistry; D007814:Larva / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D009132:Muscles / Q000276:immunology / Q000378:metabolism / Q000469:parasitology; D014234:Trichinella / Q000166:cytology / Q000276:immunology* / Q000648:ultrastructure; D014235:Trichinellosis / Q000175:diagnosis / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D006046:Gold