title: Quantitative determination of albumin in urine by on-line immunoadsorptive cleanup and reversed-phase chromatography.
abstract: Albumin in urine is selectively adsorbed on an immunoadsorber (human serum albumin-specific antibodies coupled covalently with a silica stationary phase) and after elution with 0.1% HCl is quantitatively determined by reversed-phase chromatography with detection of native fluorescence. The optimization of sample preparation and characteristics of the method such as recovery, linearity, reproducibility, detection limit, and selectivity are discussed.
authors.affiliation: Boehringer-Mannheim GmbH, Department DB-VA, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Reh
authors.forename: M
authors.initials: M
authors.lastname: Kratzer
authors.forename: D
authors.initials: D
authors.lastname: Zdunek
authors.forename: F
authors.initials: F
authors.lastname: Lang
mesh_terms: D000419:Albuminuria; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007163:Immunosorbent Techniques; D015203:Reproducibility of Results