Collagen mRNA expression detected by in situ hybridization in keloid tissue.
The keloid fibroblasts exhibited increased extracellular matrix gene expression, and prominent elevated type I procollagen mRNA when compared to control fibroblasts cultured from the uninvolved skin of normal people. It also showed markedly elevated type I/III procollagen mRNA ratios, but no synthesis of type IV procollagen mRNA by keloid fibroblasts was observed. By in situ hybridization in keloid tissue, high levels of type I and type III procollagen mRNAs were detected in most of the fibroblasts, suggesting the presence of a subpopulation responsible for the increased collagen production. The levels of type I and type III procollagen mRNAs in these fibroblasts were clearly elevated compared to control skin specimens. And concentration of type I procollagen mRNA was found more predominantly than was type III. These results suggest that deposition of collagen in keloid could result from activation of certain fibroblasts responsible for type I procollagen production.
Lee
K S
KS
Department of Dermatology, Keimyung University School of Medicine, Taegu, Korea.
Song
J Y
JY
Suh
M H
MH
D000328:Adult; D002478:Cells, Cultured; D003094:Collagen / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D007627:Keloid / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D003094:Collagen