A technique for detecting antifungal activity of proteins separated by polyacrylamide gel electrophoresis.
A technique was developed for the detection of antifungal activity of proteins after discontinuous polyacrylamide gel electrophoresis under native conditions. The antifungal activity is detected as growth inhibition zones in a homogeneous fungal lawn, grown in an agar layer spread on top of the polyacrylamide gel. The position of proteins with antifungal activity can be determined on a diffusion blot prepared from the same gel. The technique is illustrated for three antifungal plant proteins, i.e. alpha-purothionin, Urtica dioica agglutinin, and tobacco chitinase.
De Bolle
M F
MF
F. A. Janssens Laboratory of Genetics, Catholic University of Leuven, Heverlee, Belgium.
Goderis
I J
IJ
Terras
F R
FR
Cammue
B P
BP
Broekaert
W F
WF
D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides; D002688:Chitinases / Q000737:chemistry / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D037102:Lectins / Q000737:chemistry / Q000494:pharmacology; D037121:Plant Lectins; D010940:Plant Proteins / Q000737:chemistry* / Q000494:pharmacology; D010947:Plants, Toxic; D014026:Nicotiana / Q000201:enzymology
D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; C016085:purothionin; D002688:Chitinases