title: Complement subcomponent C1q stimulates Ig production by human B lymphocytes.
abstract: The regulation of Ig production by human B lymphocytes is a complex process involving interactions among B cells, APC, T lymphocytes and soluble factors including activation, growth, and differentiation factors. Components of the complement system, including C3a, C3b, C3d, and C5a, have been shown to influence various stages in this process. In this study, we demonstrate that the C1q subcomponent of complement binds to both small resting and large activated B cells and stimulates immunoglobulin production by Staphylococcus aureus Cowan-activated tonsillar B lymphocytes. This effect is present whether C1q is added to the B cells either at the beginning or near the end of a 7-day culture period and is not associated with enhancement of proliferation. The C1q stimulation of Ig production is, however, associated with increased steady state levels of mRNA for the mu Ig H chain. Furthermore, C1q stimulated IgM production by the human B cell line SKW 6.4, which is capable of secreting IgM in response to B cell differentiation factors (BCDF). SLE is a disorder frequently associated with polyclonal activation of B lymphocytes. We studied the effect of C1q on B cells from two patients with this disorder and one with an SLE-like illness, all selected for the predominance of either IgM or IgG in serum. Spontaneous or BCDF-stimulated Ig secretion was of the isotype predominant in vivo, whereas C1q selectively stimulated B cells to produce the other isotype (IgG vs IgM). Thus, C1q interacts with B lymphocytes in a manner distinct from that of BCDF found in mixed lymphocyte supernatants. C1q may be an important factor influencing the production of Ig by B lymphocytes in normal individuals and in patients with abnormalities of B cell activity.
authors.affiliation: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
authors.forename: K R
authors.initials: KR
authors.lastname: Young
authors.forename: J L
authors.initials: JL
authors.lastname: Ambrus
authors.forename: A
authors.initials: A
authors.lastname: Malbran
authors.forename: A S
authors.initials: AS
authors.lastname: Fauci
authors.forename: A J
authors.initials: AJ
authors.lastname: Tenner
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D015922:Complement C1q / Q000502:physiology*; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007148:Immunoglobulin mu-Chains / Q000096:biosynthesis; D007136:Immunoglobulins / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D012333:RNA, Messenger / Q000032:analysis; D013211:Staphylococcus aureus / Q000276:immunology
chemical_list: D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains; D007136:Immunoglobulins; D012333:RNA, Messenger; D015922:Complement C1q