Scanning and transmission electron microscopic observations of chick primordial germ cells with special reference to the extravasation in their migration course.
Chick primordial germ cells (PGCs) in the final course of their migration were observed by scanning (SEM) and transmission (TEM) electron microscopy, with an emphasis on their extravasation. The profile of the PGCs leaving blood vessels were first revealed under SEM. Chick embryos at stages 16 and 17 (about 2.5 days of incubation) were employed for the present study, since the PGCs emerging out of the vessels in teh gonadal area could be observed in high frequency at this developmental period. PGCs in the vessels showed a round profile, possessing many microvilli, while extravasating PGCs were rather oligovillous except the one side of the cell, where long filopodia extended toward the wall of the vessels. These filopodia seem to adhere to the wall of the vessels prior to emerging out. After extravasation, PGCs moved toward adjacent prospective gonadal epithelium and invaded it by amoeboidism. Following the settling down in the epithelium, PGCs showed rugged surface with few microvilli.
Ukeshima
A
A
College of Medical Science Kumamoto University, Japan.
Yoshinaga
K
K
Fujimoto
T
T
D000818:Animals; D001808:Blood Vessels / Q000166:cytology / Q000648:ultrastructure; D002465:Cell Movement; D002642:Chick Embryo; D004847:Epithelial Cells; D004848:Epithelium / Q000648:ultrastructure; D005854:Germ Cells / Q000166:cytology / Q000648:ultrastructure*; D006066:Gonads / Q000166:cytology / Q000196:embryology / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008871:Microvilli / Q000648:ultrastructure; D009024:Morphogenesis