title: Mutant analysis of interaction of the Bacillus subtilis transcription regulator AbrB with the antibiotic biosynthesis gene tycA.
abstract: The AbrB protein of B. subtilis represses the transcription of various postexponentially expressed genes, such as the antibiotic biosynthesis gene tycA. Recently, we have shown that ArbB binds to the tycA promoter region at two A + T-rich sites; the 'promoter site' (-60 to -35) and the 'leader site' (+169 to +231). In this study we demonstrate that a Ptyc-lacZ fusion missing the leader region is constitutively expressed in wild-type B. subtilis cells and in B. subtilis cells carrying spoOA or abrB mutations. We also show that substitution mutations within the recently reported potential helix-turn-helix DNA binding motif of AbrB did not affect its specific DNA binding ability.
authors.affiliation: Institut für Biochemie und Molekulare Biologie, Berlin, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Fürbass
authors.forename: M A
authors.initials: MA
authors.lastname: Marahiel
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D004269:DNA, Bacterial / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D011487:Protein Conformation; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: C060762:AbrB protein, Bacillus subtilis; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C080950:Spo0A protein, Bacillus subtilis; D014157:Transcription Factors