High-resolution banding in chromosomes of B lymphoblastoid cells and cultured skin fibroblasts.
High-resolution banded chromosomes were obtained in Epstein-Barr virus-transformed lymphoblastoid cells and cultured skin fibroblasts by aphidicolin synchronization, followed by inhibition of chromosome condensation by ethidium bromide. With this method, the chromosomes of 33.3% of lymphoblastoid cells and 48.7% of skin fibroblasts were high-resolution banded, and their mitotic indices were 1.5 and 2.7 times higher than those of control cultures.
Kuwano
A
A
Department of Pediatrics, Yamaguchi University School of Medicine, Ube, Japan.
Kajii
T
T
D016590:Aphidicolin; D001402:B-Lymphocytes / Q000648:ultrastructure*; D002478:Cells, Cultured; D002871:Chromosome Banding / Q000379:methods*; D004224:Diterpenes / Q000494:pharmacology; D004996:Ethidium / Q000494:pharmacology; D005347:Fibroblasts / Q000648:ultrastructure*; D006801:Humans; D066298:In Vitro Techniques
D004224:Diterpenes; D016590:Aphidicolin; D004996:Ethidium