Immunoelectron localization of mitochondrial F1 factor in cryosections of heart muscle cells.
Immunocytochemistry was used to investigate the localization of F1 ATPase in mitochondria of cryosections of adult mouse heart muscle cells. The initial aldehyde fixation was the only denaturation step for antigens. The fine structure was preserved with contrast enhancement as the sections were maintained hydrated, with the advantage that the entire procedure is completed in one working day. The reaction was highly specific, and entire mitochondria were labeled with the Protein A-gold complex. A new analytical technique, electron spectroscopic imaging (ESI), contributed to a better visualization of the localization of the F1 factor.
Real
S C
SC
Departamento de Ultraestrutura e Biologia Celular, Instituto Oswaldo Cruz-Fiocruz, Avenida Brasil, Rio de Janeiro.
de Meirelles
M N
MN
D000818:Animals; D004577:Electron Probe Microanalysis; D005404:Fixatives; D051379:Mice; D016253:Microscopy, Immunoelectron; D008867:Microtomy; D008929:Mitochondria, Heart / Q000737:chemistry* / Q000648:ultrastructure; D009206:Myocardium / Q000648:ultrastructure; D011506:Proteins / Q000032:analysis* / Q000648:ultrastructure; D013048:Specimen Handling; D000097795:ATPase Inhibitory Protein
D005404:Fixatives; D011506:Proteins; D000097795:ATPase Inhibitory Protein