[Synthesis of small peptides containing hydroxy-amino-acid, and its effects on progesterone production].
Eleven peptides containing hydroxy-amino-acid have been synthesized manually by stepwise solid-phase method. Three of them were started on BHA-resin, the others on Merrifield-resin. TFMSA/TFA/p-cresol were used as cleaving reagent in all peptide-resin (1-11) cleavage. Furthermore, HF cleaving procedure was also used parallelly to five of those peptide-resins for contrast. No conspicuous difference in yield was found between TFMSA and HF. The purity of all products was checked by the profiles of analytical reversed phase HPLC (C-18) and the data of amino acid analysis. All synthetic peptides were tested for the effect on progesterone production by rat corpus luteum in vitro. Among them, three peptides, GlyTyr-NH2, LysTyr-NH2 and GlySer Lys-OH, showed significant effect (p less than 0.01) on inhibiting hCG-induced progesterone production.
Wang
D X
DX
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Lu
G S
GS
Liu
W
W
Wang
N G
NG
Guan
M Z
MZ
Zhao
Y L
YL
Wang
X N
XN
Cheng
Z P
ZP
D000596:Amino Acids / Q000138:chemical synthesis* / Q000494:pharmacology; D000818:Animals; D003283:Contraceptives, Postcoital, Synthetic / Q000138:chemical synthesis*; D005260:Female; D008184:Luteal Cells / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013329:Structure-Activity Relationship
D000596:Amino Acids; D003283:Contraceptives, Postcoital, Synthetic; D011374:Progesterone