Effects of rrSCF on multiple cytokine responsive HPP-CFC generated from SCA+Lin- murine hematopoietic progenitors.
We have previously defined a subset of High Proliferative Potential Colony Forming Cells (HPP-CFC), derived from murine marrow purified for early progenitors expressing the Stem Cell Antigen (SCA+) and lacking terminal lineage markers (Lin-), which are responsive to multiple cytokines in combination. Stem Cell Factor (SCF), a multipotent growth factor which is the ligand for c-kit, synergizes with these multiple factor combinations to increase colony number and size. SCF is thus a potent mitogen with direct action on early hematopoietic progenitor cells. These data are consistent with a model of stromal control of hematopoiesis via elaboration of multiple cytokines in locally high concentration, with SCF playing a central role.
Lowry
P A
PA
Department of Internal Medicine (Hematology/Oncology), University of Virginia Health Sciences Center, Charlottesville 22908.
Zsebo
K M
KM
Deacon
D H
DH
Eichman
C E
CE
Quesenberry
P J
PJ
D000818:Animals; D000941:Antigens / Q000502:physiology*; D001854:Bone Marrow Cells; D003114:Colony-Forming Units Assay; D004357:Drug Synergism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D007375:Interleukin-1 / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology*; D051379:Mice
D000941:Antigens; D007375:Interleukin-1; D007377:Interleukin-3; D016178:Granulocyte-Macrophage Colony-Stimulating Factor