Crush and smear technique for rapid detection and semiquantitation of amyloid deposition.
A method using Congo red to rapidly identify and semiquantitate amyloid deposits in tissues for experimental research and clinical medicine is described. Examination by polarization microscopy revealed amyloid deposits as bright green birefringent clumps on a dark red background. On semiquantitative evaluation, good correlation was found between this technique and the conventional histological one, the present technique being more sensitive. The method described saves time and expense.
Shtrasburg
S
S
Heller Institute of Medical Research, Sheba Medical Center, Tel Hashomer, Israel.
Gal
R
R
Pras
M
M
D000682:Amyloid / Q000032:analysis*; D000818:Animals; D001921:Brain / Q000033:anatomy & histology; D003224:Congo Red; D006321:Heart / Q000033:anatomy & histology; D006652:Histological Techniques; D006801:Humans; D007668:Kidney / Q000737:chemistry; D008297:Male; D051379:Mice; D008859:Microscopy, Polarization; D013154:Spleen / Q000737:chemistry*
D000682:Amyloid; D003224:Congo Red