Effect of hyaluronic acid on postoperative intraperitoneal adhesion formation in the rat model.
OBJECTIVE
The aim of this study was to determine the effectiveness of hyaluronic acid solution in preventing intraperitoneal (IP) adhesions.


METHODS
The study design was prospective, randomized and blinded and involved 83 rats.


METHODS
Measured serosal injury was inflicted using a CO2 laser on the right uterine horn of the rat. Animals randomized to groups 1 and 2 received either 0.4% hyaluronic acid or its diluent phosphate-buffered saline (PBS) intraperitoneally before and after the injury. In groups 3 and 4, the same solutions were used only after the injury. Postoperative adhesions were assessed at second-look laparotomy. Histologic assessment of the fresh laser injury was carried out on uteri pretreated with hyaluronic acid, PBS, or nothing.


RESULTS
Pretreatment with hyaluronic acid was associated with a significant reduction in postoperative adhesions and a significantly decreased crater depth.


CONCLUSIONS
Hyaluronic acid appears to reduce postoperative IP adhesion formation by coating the serosal surfaces and decreasing the extent of initial tissue injury.
Urman
B
B
Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, Canada.
Gomel
V
V
Jetha
N
N
D000818:Animals; D005260:Female; D006820:Hyaluronic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D007834:Lasers; D010532:Peritoneal Diseases / Q000517:prevention & control*; D011183:Postoperative Complications; D011292:Premedication; D011833:Radiation Injuries, Experimental / Q000150:complications; D051381:Rats; D011919:Rats, Inbred Strains; D000267:Tissue Adhesions / Q000517:prevention & control*; D014599:Uterus / Q000293:injuries / Q000473:pathology / Q000528:radiation effects
D006820:Hyaluronic Acid