Arachidonic acid suppression of fatty acid synthase gene expression in cultured rat hepatocytes.
Rat hepatocytes were maintained in a serum-free, hormonally defined medium supplemented with 50-500 microM albumin-bound 20:1 (n-9) vs 20:4 (n-6). The induction of fatty acid synthase mRNA by a mix of insulin/dexamethasone/T3 was inhibited in a dose dependent fashion by 20:4 (n-6). The abundance of beta-actin mRNA was not suppressed by 20:4 (n-6). The expression of fatty acid synthase was actually stimulated 2-fold by 20:1 (n-9). It would appear that the in vivo inhibition of fatty acid synthase gene expression by dietary polyunsaturated fatty acids is a specific hepatocelluar event.
Armstrong
M K
MK
Unit of Performance Enhancement, Upjohn Company, Kalamazoo, MI 49001.
Blake
W L
WL
Clarke
S D
SD
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology*; D015152:Blotting, Northern; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D064429:Fatty Acid Synthases / Q000235:genetics*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D014284:Triiodothyronine / Q000494:pharmacology
D001095:Arachidonic Acids; D005231:Fatty Acids, Unsaturated; D007328:Insulin; D012333:RNA, Messenger; D014284:Triiodothyronine; D016718:Arachidonic Acid; D003907:Dexamethasone; D064429:Fatty Acid Synthases