Application of schlieren optics to real-time monitoring of protein electrophoresis in crosslinker-free linear polyacrylamide solution.
Molecular sieving effect of crosslinker-free linear polyacrylamide solutions could be visualized in a real-time mode using schlieren optics for electrophoresis of proteins. Since linear polymer solutions are going to find application in capillary electrophoresis (Zhu, M., et al., J. Chromatogr. 1989, 480, 311-319), the above approach may be useful for obtaining basic data of electrophoresis of proteins and nucleic acids in such media under conditions free from the predominant wall effect pertinent to the use of a capillary.
Takagi
T
T
Institute for Protein Research, Osaka University, Japan.
Kubota
H
H
Oishi
S
S
D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods; D055095:Optics and Photonics; D011506:Proteins / Q000737:chemistry*
D003432:Cross-Linking Reagents; D011506:Proteins