Regulation of the apolipoprotein AI gene by ARP-1, a novel member of the steroid receptor superfamily.
Apolipoprotein AI (apoAI) is a lipid-binding protein that participates in the transport of cholesterol and other lipids in the plasma. A complementary DNA clone for a protein that bound to regulatory elements of the apoAI gene was isolated. This protein, designated apoAI regulatory protein-1 (ARP-1), is a novel member of the steroid hormone receptor superfamily. ARP-1 bound to DNA as a dimer, and its dimerization domain was localized to the COOH-terminal region. ARP-1 also bound to a thyroid hormone-responsive element and to regulatory regions of the apoB, apoCIII, insulin, and ovalbumin genes. In cotransfection experiments, ARP-1 downregulated the apoAI gene. The involvement of ARP-1 in the regulation of apoAI gene expression suggests that it may participate in lipid metabolism and cholesterol homeostasis.
Ladias
J A
JA
Department of Cardiology, Children's Hospital, Boston, MA.
Karathanasis
S K
SK
D000595:Amino Acid Sequence; D000818:Animals; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000235:genetics*; D001483:Base Sequence; D051837:COUP Transcription Factor II; D051836:COUP Transcription Factors; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D008075:Lipoproteins, HDL / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011987:Receptors, Steroid / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D051837:COUP Transcription Factor II; D051836:COUP Transcription Factors; D004247:DNA; D004268:DNA-Binding Proteins; D008075:Lipoproteins, HDL; D015345:Oligonucleotide Probes; D011987:Receptors, Steroid; D014157:Transcription Factors; C095569:NR2F2 protein, human