title: Prospective multicenter study on isolation of human immunodeficiency virus type 1 from homosexual men after seroconversion.
abstract: A prospective multicenter study was undertaken to isolate human immunodeficiency virus type 1 (HIV-1) from 45 homosexual men for a period of 30 months after seroconversion. Efficiency of HIV-1 isolation from peripheral blood mononuclear cells was relatively stable over time, ranging from 64% at the time of seroconversion to more than 82% after 18 months of seroconversion. However, Kaplan-Meier analysis of HIV-1 culture data indicates that the cumulative proportion of HIV-1 culture positivity at 3, 6, 12, and 18 months after seroconversion was 62, 65, 84, and 92%, respectively. No significant correlation was observed between the presence of HIV-1 p24 antigen in serum, or numbers of CD4+ and CD8+ blood lymphocytes, and HIV-1 isolation within this period of time. These data suggest that HIV-1 viremia in homosexual men gradually increases to almost 100% culture positivity by 18 months after seroconversion.
authors.affiliation: Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pennsylvania 15261.
authors.forename: P
authors.initials: P
authors.lastname: Gupta
authors.forename: H
authors.initials: H
authors.lastname: Farzadegan
authors.forename: D
authors.initials: D
authors.lastname: Imagawa
authors.forename: M
authors.initials: M
authors.lastname: Lee
authors.forename: L
authors.initials: L
authors.lastname: Kingsley
authors.forename: S
authors.initials: S
authors.lastname: Zhou
authors.forename: J
authors.initials: J
authors.lastname: Armstrong
authors.forename: C
authors.initials: C
authors.lastname: Rinaldo
mesh_terms: D006679:HIV Seropositivity / Q000097:blood / Q000276:immunology / Q000382:microbiology*; D015497:HIV-1 / Q000302:isolation & purification*; D006716:Homosexuality; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D011446:Prospective Studies; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013997:Time Factors