Fluorescence polarization immunoassay employing immobilized antibody.
The use of an antibody immobilized on latex or silver colloid in fluorescence polarization immunoassay (FPI) is assessed. In FPI it is possible to detect antigens of high molecular weight because the molecular weight of the antibody is effectively increased. In the assay for rabbit immunoglobulin G a limit of detection lower by two orders of magnitude and an assay range wider by one order of magnitude can be obtained in comparison with conventional FPI. The detection limit is 10(-10) mol l-1 and the total assay time for one sample is 8 min. This assay combines a low detection limit with a short assay time.
Tsuruoka
M
M
TOYOBO Co. Ltd Research Center, Shiga, Japan.
Tamiya
E
E
Karube
I
I
D000818:Animals; D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000941:Antigens / Q000032:analysis / Q000737:chemistry; D015374:Biosensing Techniques; D005069:Evaluation Studies as Topic; D016231:Fluorescence Polarization Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis; D008970:Molecular Weight; D011817:Rabbits
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D007074:Immunoglobulin G