Expression of Staphylococcus aureus enterotoxin type D in Escherichia coli X1776.
The Staphylococcus aureus enterotoxin type D was cloned and expressed in Escherichia coli X1776 either as mature toxin or as a fusion with E. coli beta-galactosidase. Regulated expression was obtained and levels of toxin produced were in the order of 10(-3)-fold higher than in S. aureus.
Brown
R C
RC
PHLS Centre for Applied Microbiology and Research, Porton, Salisbury, Wiltshire, U.K.
Bingham
A H
AH
D001483:Base Sequence; D003001:Cloning, Molecular; D004768:Enterotoxins / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D013211:Staphylococcus aureus / Q000378:metabolism*; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D004768:Enterotoxins; D011993:Recombinant Fusion Proteins; C019368:enterotoxin D, Staphylococcal; D001616:beta-Galactosidase