Dual-column high-performance liquid chromatographic cleanup procedure for the determination of polychlorinated dibenzo-p-dioxins and dibenzofurans in fish tissue.
A high-performance liquid chromatographic cleanup procedure employing normal-phase alumina and carbon--silica separations was developed for isolating polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs) from other polychlorinated pollutants present in fish tissue. The method utilizes a column-switching step where the dioxins and furans are trace enriched onto a carbon-silica column as they are eluted from the alumina column. Interfering components such as polychlorinated biphenyls and chlorinated diphenyl ethers elute through the carbon--silica column. The PCDDs and PCDFs are subsequently recovered by backflushing the carbon--silica column using toluene.
Thompson
T S
TS
Ministry of the Environment, Dioxin Laboratory, Rexdale, Ontario, Canada.
Kolic
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TM
MacPherson
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KA
D000818:Animals; D001572:Benzofurans / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004147:Dioxins / Q000032:analysis*; D005399:Fishes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000032:analysis; D011108:Polymers / Q000032:analysis*; D013053:Spectrophotometry; D014874:Water Pollutants, Chemical / Q000032:analysis*
D001572:Benzofurans; D004147:Dioxins; D000072317:Polychlorinated Dibenzodioxins; D011108:Polymers; D014874:Water Pollutants, Chemical; C016068:polychlorodibenzofuran; C020887:dibenzo(1,4)dioxin