Basic fibroblast growth factor upregulates steady-state levels of laminin B1 and B2 chain mRNA in cultured neuroepithelial cells.
The growth of purified populations of murine neuroepithelial cells isolated from 10 day embryonic (E10) telencephalon and mesencephalon can be specifically enhanced by supplementing growth culture media with basic fibroblast growth factor (bFGF). One effect of bFGF on cultured neuroepithelial cells was to enhance the amount of laminin expressed at the protein level as detected by immunofluorescence. This was correlated with significant upregulation of steady-state levels of laminin B1 and B2 chain expression as analyzed at the mRNA level. When E12 neuroepithelial cells were split into precursor neuronal or glial subpopulations on the basis of differential expression of major histocompatibility class-1 antigens, only the glial progenitor fraction was found to be capable of detectable laminin synthesis. It is thus possible that a primary action of FGF is to increase the synthesis and release of extracellular matrix molecules from neural cells which act back in a paracrine manner to stimulate differentiation.
Drago
J
J
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
Nurcombe
V
V
Pearse
M J
MJ
Murphy
M
M
Bartlett
P F
PF
D000818:Animals; D002478:Cells, Cultured; D004475:Ectoderm / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D004622:Embryo, Mammalian; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D007797:Laminin / Q000235:genetics*; D046911:Macromolecular Substances; D051379:Mice; D008808:Mice, Inbred CBA; D010641:Phenotype; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
D007797:Laminin; D046911:Macromolecular Substances; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2