Anti-idiotype-induced, lipopolysaccharide-specific antibody response to Pseudomonas aeruginosa. II. Isotype and functional activity of the anti-idiotype-induced antibodies.
We recently developed a murine anti-idiotypic mAb that functioned as a molecular mimic of the O-specific polysaccharide side chain (Ps) of Pseudomonas aeruginosa LPS in vitro, and which induced Ps-specific antibodies in syngeneic BALB/c ByJ mice. In the current studies, we demonstrate that these anti-Id-induced, Ps-specific antibodies fix complement to the bacterial cell surface, and protect neutropenic mice from fatal P. aeruginosa sepsis. The isotypic distribution of the anti-Id-induced antibodies, however, resembles the restricted pattern (IgM and IgG3) seen after administration of purified Ps to mice. The immunogenicity and number of isotypes of Ps-specific antibody produced could be enhanced by conjugating the anti-Id to keyhole limpet hemocyanin. Finally, the anti-Id administered before immunization with purified Ps, primed BALB/c ByJ mice for production of other Ps-specific antibody isotypes (IgG1). These studies show that this anti-Id induces functional anti-Ps antibodies in syngeneic mice, and when used in conjugate form or as a priming agent before Ps immunization, yields an antibody response consistent with "T cell dependence." These immunization strategies may be useful for the induction of polysaccharide-specific antibodies in man.
Schreiber
J R
JR
Department of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, OH 44106.
Nixon
K L
KL
Tosi
M F
MF
Pier
G B
GB
Patawaran
M B
MB
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000276:immunology*; D003168:Complement Fixation Tests; D006433:Hemocyanins / Q000276:immunology; D007116:Immunization, Passive; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007132:Immunoglobulin Isotypes / Q000276:immunology; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011552:Pseudomonas Infections / Q000517:prevention & control*; D011550:Pseudomonas aeruginosa / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D007130:Immunoglobulin Idiotypes; D007132:Immunoglobulin Isotypes; D008070:Lipopolysaccharides; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin