Use of nitrocellulose membrane to activate and measure insect prophenol oxidase.
A method for the activation and measurement of insect prophenol oxidase using nitrocellulose membrane is presented. Using this method we were able to conveniently activate both crude and purified prophenol oxidase from insects belonging to three different orders. This rapid method allows for prophenol oxidase activation, in the absence of a prophenol oxidase-activating system, and in the presence of high ionic strength, protease inhibitors, or chelator.
Brey
P T
PT
Biochemistry-Laboratory, Hokkaido University, Sapporo, Japan.
Stoltz
D B
DB
Cook
D I
DI
Ashida
M
M
D000330:Aedes / Q000201:enzymology; D000818:Animals; D012831:Bombyx / Q000201:enzymology; D004156:Catechol Oxidase / Q000378:metabolism*; D003101:Collodion / Q000378:metabolism*; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000378:metabolism*; D006110:Grasshoppers / Q000201:enzymology; D007313:Insecta / Q000201:enzymology*; D007700:Kinetics; D008567:Membranes, Artificial
D004792:Enzyme Precursors; D008567:Membranes, Artificial; D003101:Collodion; C038451:pro-phenoloxidase; D004156:Catechol Oxidase