Widespread distribution of Gq alpha/G11 alpha detected immunologically by an antipeptide antiserum directed against the predicted C-terminal decapeptide.
Antisera were raised to a synthetic peptide which represents the predicted C-terminal decapeptide of the alpha subunit of the G-proteins Gq and G11. Competitive ELISA indicated that antiserum CQ2 displayed strong reactivity against this peptide. Antiserum CQ2 identified an apparently single polypeptide of 42 kDa which was expressed widely. The mobility of this polypeptide in SDS-PAGE was not modified by pretreatment of cells with pertussis toxin, indicating that it was not a substrate for this toxin. Furthermore, the levels and mobility of this polypeptide were unaltered by treatment of cells with cholera toxin, defining that it was not related to Gs alpha.
Mitchell
F M
FM
Department of Biochemistry, University of Glasgow, Scotland, UK.
Mullaney
I
I
Godfrey
P P
PP
Arkinstall
S J
SJ
Wakelam
M J
MJ
Milligan
G
G
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D002540:Cerebral Cortex / Q000737:chemistry; D002772:Cholera Toxin / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000737:chemistry; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000276:immunology; D005910:Glioma / Q000737:chemistry; D006801:Humans; D006822:Hybrid Cells / Q000737:chemistry; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D008969:Molecular Sequence Data; D009447:Neuroblastoma / Q000737:chemistry; D010446:Peptide Fragments / Q000276:immunology*; D037342:Pertussis Toxin; D051381:Rats; D014407:Tumor Cells, Cultured; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D007106:Immune Sera; D010446:Peptide Fragments; D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D002772:Cholera Toxin; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins