Unusual topography of bovine rhodopsin promoter-lacZ fusion gene expression in transgenic mouse retinas.
To define the cis-acting DNA elements required for rhodopsin expression, we generated lines of transgenic mice carrying sequences upstream of the bovine rhodopsin gene fused to the E. coli beta-galactosidase gene (lacZ). Upstream sequences extending from -2174 to +70 bp, from -734 to +70 bp, and from -222 to +70 bp direct photoreceptor-specific expression. All three -2174 lines demonstrate a superior-temporal to inferior-nasal gradient of expression across the retina, whereas lines carrying the shorter constructs demonstrate either spatially continuous expression across the retina, discrete clusters of expression, or both. As a complementary approach to defining regulatory elements, we compared DNA sequences 5' of the murine, bovine, and human rhodopsin genes. Significant homology between all three species was found just upstream of the transcription start site and at approximately 1.5 kb upstream.
Zack
D J
DJ
Department of Molecular Biology and Genetics, Johns Hopkins Medical School, Baltimore, Maryland 21205.
Bennett
J
J
Wang
Y
Y
Davenport
C
C
Klaunberg
B
B
Gearhart
J
J
Nathans
J
J
D000375:Aging / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle / Q000235:genetics*; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D008969:Molecular Sequence Data; D010786:Photoreceptor Cells / Q000502:physiology; D011401:Promoter Regions, Genetic; D012160:Retina / Q000502:physiology*; D012243:Rhodopsin / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D001616:beta-Galactosidase / Q000235:genetics* / Q000378:metabolism
D012243:Rhodopsin; D001616:beta-Galactosidase