Protein phosphorylation in isolated nuclei from etiolated Avena seedlings. Effects of red/far-red light and cholera toxin.
We have studied the phosphorylation/dephosphorylation of several nuclear proteins in isolated nuclei from etiolated Avena seedlings as a function of red/far-red light. The effect of stimulatory (ADP-ribosylation by cholera toxin) or inhibitory (GDP beta S) conditions for GTP-binding proteins was also studied. Red or far-red light enhanced the phosphorylation level of 2 nuclear proteins with molecular masses of 75 and 60 kDa. The phosphorylation pattern was affected by the addition of cholera toxin or GDP beta S to the isolated nuclei. At least 2 proteins with molecular masses of 24 and 75 kDa cross-reacted by Western blot with GTP-binding protein antibodies.
Romero
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Institute for Cellular and Molecular Photobiology, University of Nebraska, Lincoln 68588-0304.
Biswal
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Song
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D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism; D002772:Cholera Toxin / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000502:physiology; D006153:Guanosine Diphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D008027:Light; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D006109:Poaceae / Q000378:metabolism*; D013873:Thionucleotides / Q000494:pharmacology
D009687:Nuclear Proteins; D010750:Phosphoproteins; D010940:Plant Proteins; D013873:Thionucleotides; D006153:Guanosine Diphosphate; C023427:guanosine 5'-O-(2-thiodiphosphate); D002772:Cholera Toxin; D019204:GTP-Binding Proteins