Metastatic T-cell hybridoma cells display target preference for invasion ('homing') in tissue culture models.
BW-O-Li1 murine T-lymphoma cells display target preference for invasion in two different in vitro models. In the precultured chick heart fragment (PHF) assay, BW-O-Li1 preferentially invaded into aggregates of MCF-7 mammary carcinoma cells rather than into PHFs. In a monolayer invasion assay (MIA), BW-O-Li1 cells preferentially invaded under 10 T1/2 mouse embryo cell monolayers rather than under MCF-7 monolayers. Thus, although BW-O-Li1 cells were perfectly able to invade into any of the targets presented, they migrated and accumulated preferentially in one of the targets when a choice was offered. We suggest that this in vitro 'homing' phenomenon can be exploited to investigate certain aspects of organ-specific metastasis.
Verschueren
H
H
Pasteur Instituut van Brabant, Brussels, Belgium.
De Bruyne
G K
GK
Dewit
J
J
De Baetselier
P
P
Mareel
M M
MM
D000818:Animals; D002465:Cell Movement; D002645:Chickens; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000473:pathology*; D016399:Lymphoma, T-Cell / Q000473:pathology*; D051379:Mice; D009206:Myocardium / Q000473:pathology; D009361:Neoplasm Invasiveness; D014407:Tumor Cells, Cultured