title: Protein kinase C activity and intracellular distribution in surgically excised human epileptic neocortex.
abstract: Protein kinase C (PKC) activity assayed by phosphorylation of exogenous histone, was measured in neocortex obtained from 32 patients following surgery for focal epilepsy and from 6 non-epileptic patients. PKC activity was not significantly different in either the particulate or cytosolic fraction from epileptic foci (n = 17) versus samples from non-spiking regions (n = 22) or neocortex from non-epileptic patients (n = 6). From 67% to 70% of total PKC activity was present in the cytosolic fraction. Phosphorylation of endogenous cytosolic substrate proteins was also not significantly different in epileptic foci.
authors.affiliation: Montreal Neurological Institute, McGill University, Qué, Canada.
authors.forename: O
authors.initials: O
authors.lastname: Vernet
authors.forename: V W
authors.initials: VW
authors.lastname: Yong
authors.forename: K
authors.initials: K
authors.lastname: Rostworowski
authors.forename: M
authors.initials: M
authors.lastname: Fadich
authors.forename: A
authors.initials: A
authors.lastname: Olivier
authors.forename: A L
authors.initials: AL
authors.lastname: Sherwin
mesh_terms: D001706:Biopsy; D002540:Cerebral Cortex / Q000201:enzymology* / Q000473:pathology; D004827:Epilepsy / Q000201:enzymology*; D006801:Humans; D011493:Protein Kinase C / Q000378:metabolism*
chemical_list: D011493:Protein Kinase C