Delta 5-desaturation of dihomogammalinolenic acid (20:3(n-6)) into arachidonic acid (20:4(n-6)) by rat liver microsomes and incorporation of fatty acids in microsome phospholipids.
Liver microsomes of rats fed an essential fatty acid (EFA)-deficient diet or a commercial balanced diet were used to study the effect of incubation time on the delta 5-desaturation of [14C]dihomogammalinolenic acid (20:3(n-6)) into arachidonic acid (20:4(n-6)) and incorporation of the two acids into microsomal phospholipids. The EFA-deficient diet highly increased the desaturation rate of 20:3(n-6). Incorporation of the formed 20:4(n-6) into microsomal phospholipids was also increased but at saturating concentration of substrate only. At early times of incubation, the precursor 20:3(n-6) was rapidly incorporated into phospholipids. Formation and incorporation of 20:4(n-6) into phospholipids proceeded more progressively. Data suggest that desaturation of 20:3(n-6) and incorporation of both 20:3(n-6) and 20:4(n-6) into phospholipids occur concomitantly and independently.
Blond
J P
JP
Unité de Nutrition Cellulaire et Métabolique, Université de Bourgogne, Dijon, France.
Bézard
J
J
D015126:8,11,14-Eicosatrienoic Acid / Q000378:metabolism*; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D005228:Fatty Acids, Essential / Q000008:administration & dosage / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
D001095:Arachidonic Acids; D004041:Dietary Fats; D005228:Fatty Acids, Essential; D010743:Phospholipids; D044943:Fatty Acid Desaturases; D015126:8,11,14-Eicosatrienoic Acid