title: Toxicity of cyst extract of Sarcocystis fusiformis from buffalo in rabbits and mice.
abstract: The toxic effect of cysts of Sarcocystis fusiformis (of buffalo origin) in rabbits and mice have been studied. It is shown that inoculation of a 2 mg ml-1 concentration of protein of the macrosarcocyst extract (ME) into rabbits and mice was lethal. The toxic substance was thermolabile at greater than or equal to 60 degrees C for 30 min. Protease and trypsin caused inactivation, while papain did not affect the toxicity. ME was found to be non-dialysable, but precipitable. There was also no effect of a pH of 6-9, while pH 4-5 altered the toxicity and pH 10 only partially affected the toxic effect.
authors.affiliation: Department of Parasitology, College of Veterinary Sciences, G.P. Pant University of Agriculture and Technology, India.
authors.forename: A
authors.initials: A
authors.lastname: Saleque
authors.forename: B B
authors.initials: BB
authors.lastname: Bhatia
authors.forename: P D
authors.initials: PD
authors.lastname: Juyal
authors.forename: H
authors.initials: H
authors.lastname: Rahman
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000469:parasitology*; D010450:Endopeptidases / Q000494:pharmacology; D005260:Female; D006358:Hot Temperature; D008297:Male; D051379:Mice; D010206:Papain / Q000494:pharmacology; D015800:Protozoan Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000633:toxicity*; D011817:Rabbits; D012522:Sarcocystis / Q000502:physiology*; D012523:Sarcocystosis / Q000469:parasitology / Q000662:veterinary*; D014118:Toxins, Biological / Q000737:chemistry / Q000633:toxicity*; D014357:Trypsin / Q000494:pharmacology
chemical_list: D015800:Protozoan Proteins; D014118:Toxins, Biological; D010450:Endopeptidases; D014357:Trypsin; D010206:Papain