Gene transfer into human leukemia cell lines by electroporation: experience with exponentially decaying and square wave pulse.
The efficiency of gene transfer into human leukemia cell lines by electroporation was investigated. For both transient expression (beta-galactosidase gene) and stable transformation (neomycin resistance gene), the transfer efficiency into leukemia cell lines using a square wave pulse was superior to that using an exponentially decaying wave. The transfer rate of pMoZtk (containing beta-galactosidase gene) into K562 by electroporation using a square wave was approximately 5%, compared with 1% by an exponentially decaying pulse. Whereas the transfer rate of pMAM-neo into K562 by electroporation using an exponentially decaying pulse was less than 10(-5), a square wave generated much more efficient introduction rate of nearly 10(-3). In the other leukemia cell lines also, some square wave yields were better than exponential yields and all square wave yields were at least as good as the exponential yields.
Takahashi
M
M
First Department of Internal Medicine, Niigata University, School of Medicine, Japan.
Furukawa
T
T
Saitoh
H
H
Aoki
A
A
Koike
T
T
Moriyama
Y
Y
Shibata
A
A
D015139:Blotting, Southern; D002455:Cell Division; D002470:Cell Survival; D004273:DNA, Neoplasm / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D004560:Electricity; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D007942:Leukemia, Experimental / Q000235:genetics*; D007951:Leukemia, Myeloid / Q000235:genetics*; D009355:Neomycin; D014162:Transfection; D014407:Tumor Cells, Cultured; D001616:beta-Galactosidase / Q000235:genetics
D004273:DNA, Neoplasm; D001616:beta-Galactosidase; D009355:Neomycin