In vitro and in vivo analysis of somatic and germline mutability of 2-amino-N6-hydroxyadenine in Drosophila melanogaster.
Two complementary assays were employed to examine the mutagenicity of 2-amino-N6-hydroxyadenine (AHA) in Drosophila melanogaster. A lambda phage-based shuttle vector system, utilizing the supF transfer RNA gene of Escherichia coli, questioned the mutagenicity of AHA in established cell cultures derived from somatic tissue while the standard sex-linked recessive lethal assay measured mutational events in vivo. Consistent with studies in other systems, AHA appears strongly mutagenic when cells are exposed directly. Conversely, in vivo studies suggest that AHA is not a strong mutagen. Further studies will determine if AHA is weakly or not mutagenic in vivo and, using the supF system, what the nature of the mutational events at the molecular level is.
Smith
P D
PD
Department of Biological Sciences, Wayne State University, Detroit, MI 48202.
Lee-Chen
S F
SF
Liljestrand
C A
CA
Dusenbery
R L
RL
D000225:Adenine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity; D000818:Animals; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics; D005804:Genes, Lethal; D016153:Genes, Suppressor; D005854:Germ Cells; D066298:In Vitro Techniques; D009152:Mutagenicity Tests; D009153:Mutagens; D012343:RNA, Transfer / Q000235:genetics; D013056:Spectrophotometry, Ultraviolet
D009153:Mutagens; C030632:2-amino-N(6)-hydroxyadenine; D012343:RNA, Transfer; D000225:Adenine