Hormone-responsive adenylyl cyclase in the human fallopian tube.
Hormone-responsive adenylyl cyclase (AC) activity in biopsies from normal human Fallopian tubes was studied. Enzyme activity with a Km of 0.15 mmol/L and a maximum velocity of 13.8 pmol/mg.min in the basal condition was demonstrated. The addition of prostaglandin E1 (PGE1), PGE2, PGF2a, vasoactive intestinal polypeptide, isoproterenol, and terbutaline increased enzyme activity, with no change in the Km. Maximum stimulation of AC activity was obtained with PGE1, resulting in a 2- to 8-fold increase in AC activity. The response of AC to PGE1 revealed a possible topographical variation, with lowest responses to PGE1 in the isthmus. No such segmental variation in AC activity and response was seen after stimulation with PGF2a, vasoactive intestinal polypeptide, or isoproterenol.
Tanbo
T
T
Department of Obstetrics and Gynecology, National Hospital, Oslo, Norway.
Bjørnerheim
R
R
Abyholm
T
T
Hansson
V
V
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000527:Alprostadil / Q000494:pharmacology; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D015237:Dinoprost / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology; D005187:Fallopian Tubes / Q000201:enzymology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D005934:Glucagon / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology*; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D013726:Terbutaline / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D006063:Chorionic Gonadotropin; D006728:Hormones; D014660:Vasoactive Intestinal Peptide; D005640:Follicle Stimulating Hormone; D005934:Glucagon; D015237:Dinoprost; D000262:Adenylyl Cyclases; D000527:Alprostadil; D015232:Dinoprostone; D007545:Isoproterenol; D013726:Terbutaline