Structural relationship between the vancomycin resistance protein VanH and 2-hydroxycarboxylic acid dehydrogenases.
Sequencing upstream from the vanA gene of enterococcal plasmid pIP816 that confers Vm resistance revealed the presence of an ORF which could code for a protein of 322 aa designated VanH. Extensive aa similarity was detected between VanH and 2-hydroxycarboxylic acid dehydrogenase. We discuss possible roles for VanH in the synthesis of a novel type of peptidoglycan precursors with lower affinity for Vm.
Arthur
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Unité des Agents Antibactériens, Institut Pasteur, Paris, France.
Molinas
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Dutka-Malen
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Courvalin
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D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D002264:Carboxylic Acids / Q000378:metabolism; D004352:Drug Resistance, Microbial; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010457:Peptidoglycan / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D016415:Sequence Alignment; D014640:Vancomycin / Q000378:metabolism / Q000494:pharmacology
D001426:Bacterial Proteins; D002264:Carboxylic Acids; D010457:Peptidoglycan; C070700:VanH protein, Enterococcus faecium; D014640:Vancomycin; D000429:Alcohol Oxidoreductases