The giemsa banding pattern of canine chromosomes, using a cell synchronization technique.
Cytogenetic investigations of the domestic dog, Canis familiaris, were performed on the Doberman pinscher and two Boxer dogs. Conventional homogeneously stained and G-banded metaphases from peripheral blood lymphocyte cultures synchronized with amethopterin and bromodeoxyuridine were studied. These procedures permitted the unequivocal identification of all canine chromosomes. A canine chromosome idiogram was constructed on the basis of the G-banding pattern at the haploid 327-band resolution level. The secondary constrictions and tapering of the telomeric regions characteristic of several canine chromosomes are described. Q-, C-, and NOR-banding were also performed and the salient features are described. This karyotype should enhance the value of the canine species in cytogenetic investigations.
Stone
D M
DM
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.
Jacky
P B
PB
Prieur
D J
DJ
D000818:Animals; D001399:Azure Stains; D002478:Cells, Cultured; D002871:Chromosome Banding / Q000379:methods; D003582:Cytogenetics / Q000379:methods; D004285:Dogs; D005260:Female; D007621:Karyotyping; D008297:Male; D008677:Metaphase; D014960:X Chromosome
D001399:Azure Stains