Functional water channels and proton pumps are in separate populations of endocytic vesicles in toad bladder granular cells.
Functional water channels are retrieved by endocytosis from the apical membrane of toad bladder granular cells in response to vasopressin [Shi, L.-B., & Verkman, A.S. (1989) J. Gen. Physiol. 94, 1101-1115]. To examine whether endocytic vesicles which contain the vasopressin-sensitive water channel fuse with acidic vesicles for entry into a lysosomal pathway, ATP-dependent acidification and osmotic water permeability were measured in endosomes from control bladders and bladders treated with vasopressin (VP) and/or phorbol myristate acetate (PMA). Endosomes were labeled with the fluid-phase markers 6-carboxyfluorescein or fluorescein-dextran. Osmotic water permeability (Pf) was measured by stopped-flow fluorescence quenching and proton ATPase activity by ATP-dependent, N-ethylmaleimide-inhibitable acidification. In a microsomal pellet, Pf was low (less than 0.002 cm/s, 20 degrees C) in labeled endocytic vesicles from control bladders but high (0.05-0.1 cm/s) in a subpopulation (50-70%) of vesicles from VP- and PMA-treated bladders. Following ATP addition, the average drop in pH was 0.1 (control), 0.3 (VP), and 0.2 (PMA) unit. Measurement of pH in individual endocytic vesicles by quantitative image analysis showed that less than 20% of vesicles from VP-treated bladders acidified by greater than 0.5 pH unit. To examine whether water channels and proton pumps were present in the same endocytic vesicles, the pH of endosomes with high and low water permeability was measured from the effect of ATP on the amplitude of the fluorescence quenching signal in response to an osmotic gradient.(ABSTRACT TRUNCATED AT 250 WORDS)
Wang
Y X
YX
Department of Medicine, University of California, San Francisco 94143.
Shi
L B
LB
Verkman
A S
AS
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002024:Bufo marinus; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D004705:Endocytosis / Q000187:drug effects; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008247:Lysosomes / Q000378:metabolism / Q000648:ultrastructure; D009550:Nigericin / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013861:Thiocyanates; D001743:Urinary Bladder / Q000166:cytology / Q000378:metabolism*; D014667:Vasopressins / Q000494:pharmacology; D014867:Water
D005452:Fluoresceins; D005456:Fluorescent Dyes; D013861:Thiocyanates; D014867:Water; D014667:Vasopressins; D000255:Adenosine Triphosphate; D016650:Fluorescein-5-isothiocyanate; D013755:Tetradecanoylphorbol Acetate; D009550:Nigericin