Avian CD4 and CD8 interact with a cellular tyrosine protein kinase homologous to mammalian p56lck.
Functional as well as immunochemical studies have indicated that chicken T lymphocytes express surface antigens homologous to mammalian CD4 and CD8. The results presented in this report demonstrate that the avian CD4 and CD8 homologs are physically associated with a cellular tyrosine protein kinase related to mammalian p56lck. Furthermore, they show that engagement of either avian CD4 or CD8 is associated with a rapid intracellular tyrosine phosphorylation signal, suggesting that the function of these surface molecules may be mediated through alterations of the associated tyrosine protein kinase. Taken together, these findings demonstrate that the associations of CD4 and CD8 with an intracellular tyrosine protein kinase are evolutionarily conserved, further enhancing the likelihood that they are critical for normal immune functions.
Veillette
A
A
Department of Biochemistry, McGill Cancer Centre, Montreal, Canada.
Ratcliffe
M J
MJ
D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology*; D015704:CD4 Antigens / Q000502:physiology*; D016827:CD8 Antigens; D002645:Chickens / Q000276:immunology*; D003488:Cyanogen Bromide; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D010449:Peptide Mapping; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000502:physiology*; D012697:Serine Endopeptidases; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D015704:CD4 Antigens; D016827:CD8 Antigens; D011505:Protein-Tyrosine Kinases; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D003488:Cyanogen Bromide