title: Characterization of a new bioactive protein from bovine seminal fluid.
abstract: A new acidic seminal fluid protein (aSFP) was purified from bovine seminal fluid, using anion exchange chromatography and FPLC on MonoQ. The purified aSFP displays a pI of 4.8 and an apparent molecular weight of 14 kDa. Homogeneity of aSFP was demonstrated by FPLC and SDS-polyacrylamide gel electrophoresis. Monospecific anti-aSFP IgGs were employed to characterize aSFP in bovine seminal plasma and seminal vesicle secretion by immuno blot analysis. Proteinchemical characterization of aSFP included amino acid analysis as well as determination of 23 amino acid residues of the N-terminal sequence of aSFP. According to this sequence, aSFP appears to represent a hitherto unknown protein. aSFP stimulated cell division and progesterone secretion of bovine granulosa cells in vitro in a potent and dose dependent manner. aSFP appears to be a potent growth factor with effects on ovarian granulosa cells.
authors.affiliation: Lehrstuhl für Physiologie der Fortpflanzung und Laktation, Technische Universität München, Freising, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Einspanier
authors.forename: A
authors.initials: A
authors.lastname: Einspanier
authors.forename: F
authors.initials: F
authors.lastname: Wempe
authors.forename: K H
authors.initials: KH
authors.lastname: Scheit
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002455:Cell Division; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011374:Progesterone / Q000378:metabolism; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins; D012669:Seminal Vesicles / Q000737:chemistry*
chemical_list: D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; D011374:Progesterone