Feasibility of using an oleate-rich diet to reduce the susceptibility of low-density lipoprotein to oxidative modification in humans.
Oxidized low-density lipoprotein (LDL) is more atherogenic than native LDL. The initial step in the oxidation is the peroxidation of polyunsaturated fatty acids. Thus, decreasing the concentration of polyunsaturated fatty acids should reduce the susceptibility of LDL to oxidation. Therefore, we tested the possibility that diets enriched in oleate might result in LDL that is less susceptible to oxidative modification. LDL isolated from subjects consuming an oleate-enriched diet, compared with LDL from subjects on a linoleate-enriched diet, contained significantly more oleate (28.7% vs 11.5%) and less linoleate (31.9% vs 50.9%). Generation of conjugated dienes was significantly lower in the LDL from the oleate group. Most important, after incubation with endothelial cells, LDL from the oleate group underwent less degradation by macrophages. These studies demonstrate the feasibility of altering the diet in a way that will not raise LDL cholesterol concentrations and yet will decrease the susceptibility of LDL to oxidative modification.
Reaven
P
P
Department of Medicine, University of California, San Diego, La Jolla 92093-0613.
Parthasarathy
S
S
Grasse
B J
BJ
Miller
E
E
Almazan
F
F
Mattson
F H
FH
Khoo
J C
JC
Steinberg
D
D
Witztum
J L
JL
D000328:Adult; D001835:Body Weight; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D015930:Diet Records; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D005227:Fatty Acids / Q000032:analysis; D006368:Helianthus; D006801:Humans; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000008:administration & dosage; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000378:metabolism*; D009829:Oleic Acids / Q000008:administration & dosage*; D010084:Oxidation-Reduction; D010349:Patient Compliance; D010938:Plant Oils / Q000008:administration & dosage; D011897:Random Allocation; D000074242:Sunflower Oil
D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D008041:Linoleic Acids; D008077:Lipoproteins, LDL; D009829:Oleic Acids; D010938:Plant Oils; D000074242:Sunflower Oil; D002784:Cholesterol; D019787:Linoleic Acid