Regulation of ketone formation and phosphoenolpyruvate carboxykinase activity in the small intestinal mucosa of infant rats.
We studied the effect of different hormones added in vivo or in vitro on ketogenesis and phosphoenolpyruvate carboxykinase (PEPCK) activity in the small intestinal mucosa of suckling rats. Injection of insulin or dexamethasone in vivo or of an antiglucagon antiserum decreased the rate of ketone formation in the mucosa whereas injection of anti-insulin antiserum led to increased mucosal ketogenesis. PEPCK activity in the mucosa was decreased by the antiglucagon serum but was not affected by insulin or anti-insulin serum injections. Both liver and brown fat PEPCK responded as expected with the activity being elevated by anti-insulin serum and depressed by both insulin and antiglucagon serum. In the in vitro experiments, no effect of any of the agents on PEPCK was found. Ketone formation was suppressed in vitro by insulin or dexamethasone addition to the medium.
Hahn
P
P
Department of Obstetrics and Gynaecology, University of British Columbia, Vancouver, Canada.
Taller
M
M
Srubiski
L
L
Kirby
L
L
D000818:Animals; D000831:Animals, Newborn; D003907:Dexamethasone / Q000494:pharmacology; D005934:Glucagon / Q000502:physiology; D006885:Hydroxybutyrates / Q000378:metabolism; D007106:Immune Sera; D007328:Insulin / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000378:metabolism; D007421:Intestine, Small / Q000201:enzymology* / Q000378:metabolism; D007657:Ketone Bodies / Q000096:biosynthesis*; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism*; D051381:Rats
D006885:Hydroxybutyrates; D007106:Immune Sera; D007328:Insulin; D007657:Ketone Bodies; D003907:Dexamethasone; D005934:Glucagon; D010729:Phosphoenolpyruvate Carboxykinase (GTP)