Characterization of human interferon-gamma and human interleukin-2 from recombinant mammalian cell lines and peripheral blood lymphocytes.
Human interleukin-2 (IL-2) and human interferon-gamma (IF-gamma) isolated from transfected heterologous cell lines were structurally compared with their natural counterparts isolated from peripheral blood lymphocytes (PBL). Several forms of IL-2 and IFN-gamma that were primarily due to differential glycosylation were produced by all cell types studied. Comparable molecular weights and sugar compositions were found for variants of natural and mammalian-derived recombinant IL-2, and variants of natural and mammalian-derived recombinant IFN-gamma. Furthermore, the specific biological activities of the natural and mammalian-derived recombinant lymphokines were similar and comparable to the specific activity of the Escherichia coli-derived recombinant proteins.
Riske
F J
FJ
Department of Molecular Genetics, Roche Research Center, Hoffman-La Roche Inc., Nutley, NJ 07110.
Cullen
B R
BR
Chizzonite
R
R
D001681:Biological Assay; D015153:Blotting, Western; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D007376:Interleukin-2 / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D008214:Lymphocytes / Q000276:immunology*; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D014162:Transfection
D007376:Interleukin-2; D011994:Recombinant Proteins; D007371:Interferon-gamma