Replication of human immunodeficiency virus type 1 in primary dendritic cell cultures.
The ability of the human immunodeficiency virus type 1 (HIV-1) to replicate in primary blood dendritic cells was investigated. Dendritic cells compose less than 1% of the circulating leukocytes and are nondividing cells. Highly purified preparations of dendritic cells were obtained using recent advances in cell fractionation. The results of these experiments show that dendritic cells, in contrast to monocytes and T cells, support the active replication of all strains of HIV-1 tested, including T-cell tropic and monocyte/macrophage tropic isolates. The dendritic cell cultures supported much more virus production than did cultures of primary unseparated T cells, CD4+ T cells, and adherent as well as nonadherent monocytes. Replication of HIV-1 in dendritic cells produces no noticeable cytopathic effect nor does it decrease total cell number. The ability of the nonreplicating dendritic cells to support high levels of replication of HIV-1 suggests that this antigen-presenting cell population, which is also capable of supporting clonal T-cell growth, may play a central role in HIV pathogenesis, serving as a source of continued infection of CD4+ T cells and as a reservoir of virus infection.
Langhoff
E
E
Division of Human Retrovirology, Dana-Farber Cancer Institute, Boston, MA 02115.
Terwilliger
E F
EF
Bos
H J
HJ
Kalland
K H
KH
Poznansky
M C
MC
Bacon
O M
OM
Haseltine
W A
WA
D002469:Cell Separation; D003713:Dendritic Cells / Q000166:cytology / Q000382:microbiology*; D015683:Gene Products, gag / Q000378:metabolism; D016655:HIV Core Protein p24; D015497:HIV-1 / Q000254:growth & development*; D006681:HLA-D Antigens / Q000378:metabolism; D006801:Humans; D008855:Microscopy, Electron, Scanning; D014758:Viral Core Proteins / Q000378:metabolism; D014779:Virus Replication
D015683:Gene Products, gag; D016655:HIV Core Protein p24; D006681:HLA-D Antigens; D014758:Viral Core Proteins