An improved method for the detection of differential survival between normal and xeroderma pigmentosum lymphoblastoid cell lines in culture with 4-nitroquinoline-1-oxide.
The effects of the UV-mimetic chemical 4-nitroquinoline-1-oxide (4-NQO) upon cell lines heterozygous or homozygous for the recessive mutant xeroderma pigmentosum (XP) were investigated. Human lymphoblastoid cell lines, which were established from 4 XP homozygote patients (XPL15, XPL17, XPL19 and XPL20). 2 XP heterozygote individuals (XPPL17 and XPML17) and 58 normal individuals, were cultured in the presence of 4-NQO at doses of 0, 2, 4 and 8 x 10(-6) M. Then the total cell number was counted and the viability of the cells was measured by the dye exclusion method using trypan blue and a newly devised fluorometric method with fluorescein diacetate. Results showed that 4-NQO affected, in increasing order of impairment, the cell lines: normal less than XP heterozygote less than XP homozygote.
Kiyohara
C
C
Department of Public Health, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Hirohata
T
T
Kuratsune
M
M
Nagayama
J
J
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007945:Leukemia, Lymphoid / Q000473:pathology; D009154:Mutation; D014983:Xeroderma Pigmentosum / Q000235:genetics / Q000473:pathology*
D015112:4-Nitroquinoline-1-oxide