Metaphase chromosomes from mammalian cells stimulate fusion of artificial phospholipid vesicles.
Isolated mitotic chromosomes are able to form complexes with phosphatidylcholine liposomes in the presence and absence of Ca2+ ions, in the latter case in the presence of polyamines. Interactions with chromosomes stimulates liposome fusion. The fusion is promoted by condensed and EDTA-decondensed chromosomes.
Balakireva
L A
LA
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences.
Belyaev
N D
ND
Budker
V G
VG
Kiseleva
E V
EV
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000378:metabolism; D000327:Adsorption; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002843:Chromatin / Q000378:metabolism; D002875:Chromosomes / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D004735:Energy Transfer; D008081:Liposomes / Q000378:metabolism*; D008561:Membrane Fusion; D008677:Metaphase; D051379:Mice; D008854:Microscopy, Electron; D010743:Phospholipids / Q000378:metabolism; D013095:Spermidine / Q000494:pharmacology
D002843:Chromatin; D008081:Liposomes; D010743:Phospholipids; D015060:1,2-Dipalmitoylphosphatidylcholine; D004247:DNA; D004492:Edetic Acid; D002118:Calcium; D013095:Spermidine