title: Mutational dissociation of the positive and negative regulatory properties of the Spo0A sporulation transcription factor of Bacillus subtilis.
abstract: The Spo0A regulatory protein controls the onset of stationary phase and sporulation by controlling transcription in both a negative and a positive manner depending on the promoter affected. Missense mutations, e.g., spo0A9V, which result in alterations in the eleventh amino acid preceding the C terminus of the Spo0A protein, give rise to a protein active as a negative regulator of the abrB gene but unable to activate transcription of the spoIIA gene. Second-site suppressors of spo0A9V occurred within the spo0A gene at codons 162 and 174. These suppressors did not suppress a spo0F mutation, indicating that the suppressed protein still requires phosphorylation for activity. The results suggest that the C terminus of Spo0A interacts with the transcription complex to activate transcription.
authors.affiliation: Department of Molecular and Experimental Medicine, Research Institute of Scripps Clinic, La Jolla, CA 92037.
authors.forename: M
authors.initials: M
authors.lastname: Perego
authors.forename: J J
authors.initials: JJ
authors.lastname: Wu
authors.forename: G B
authors.initials: GB
authors.lastname: Spiegelman
authors.forename: J A
authors.initials: JA
authors.lastname: Hoch
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D016153:Genes, Suppressor; D005838:Genotype; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009876:Operon; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D013171:Spores, Bacterial / Q000502:physiology; D013489:Suppression, Genetic; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; C080950:Spo0A protein, Bacillus subtilis; D014157:Transcription Factors