title: Isolation of a coenzyme M-auxotrophic mutant and transformation by electroporation in Methanococcus voltae.
abstract: An auxotrophic mutant of Methanococcus voltae was isolated that required coenzyme M (CoM) for growth. With the mutant as a recipient, conditions were developed that allowed the introduction of wild-type CoM+ DNA into the mutant methanogen via electroporation. This method also allowed the rescue of both a histidine and purine auxotroph as well as the introduction of DNA determining resistance to the CoM analog 2-bromoethanesulfonic acid. Electroporation of the CoM(+)-determining DNA was 50- to 80-fold more efficient than natural transformation.
authors.affiliation: Department of Microbiology, University of Illinois, Urbana 61801.
authors.forename: P A
authors.initials: PA
authors.lastname: Micheletti
authors.forename: K A
authors.initials: KA
authors.lastname: Sment
authors.forename: J
authors.initials: J
authors.lastname: Konisky
mesh_terms: D019605:Euryarchaeota / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D015080:Mesna / Q000378:metabolism*; D014170:Transformation, Genetic
chemical_list: D015080:Mesna