Elevated 18-hydroxy-corticosterone in inbred salt-sensitive rats.
Rats susceptible to the hypertensive effect of dietary salt (SS/Jr) have excess 18-hydroxydeoxycorticosterone (18-OH-DOC) and 19-nor-DOC compared to control rats (SR/Jr). This may be caused by an abnormal adrenal 11 beta-hydroxylase, which catalyzes the 11 beta, 18, and 19-hydroxylations of DOC. A comparison of the urinary products of this enzyme including 18-OH-DOC, 19-nor-DOC, corticosterone (B), and 18-OH-B have not been described in the SS/Jr. Therefore, these steroid products were measured at 7 and 12 weeks of age in 36 weanling male and female, SS/Jr and SR/Jr (n = 9 in each group), on a low-salt diet. In both the male and female SS/Jr urinary free levels of 18-OH-DOC, 19-nor-DOC, and 18-OH-B were elevated, while B was not different at 6 and 10 weeks of age. The largest increases were in 18-OH-B levels, and these levels correlated with 18-OH-DOC and B but not 19-nor-DOC. The high degree of correlation between these steroids probably reflects their closely related dependence on adrenal 11 beta-hydroxylase biosynthesis.
Griffing
G T
GT
Evans Memorial Department of Clinical Research, Boston University Medical Center, Massachusetts 02118.
Melby
J C
JC
Holbrook
M
M
Wilson
T
T
Azar
S
S
Delaney
M
M
Weiss
S
S
D015069:18-Hydroxycorticosterone / Q000652:urine*; D000311:Adrenal Glands / Q000201:enzymology; D000818:Animals; D003900:Desoxycorticosterone / Q000031:analogs & derivatives / Q000652:urine; D004351:Drug Resistance; D005260:Female; D006973:Hypertension / Q000201:enzymology / Q000209:etiology* / Q000652:urine; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride / Q000008:administration & dosage; D013252:Steroid 11-beta-Hydroxylase / Q000378:metabolism
C042509:18-hydroxydeoxycorticosterone; D003900:Desoxycorticosterone; D012965:Sodium Chloride; C019026:19-nordeoxycorticosterone; D015069:18-Hydroxycorticosterone; D013252:Steroid 11-beta-Hydroxylase