title: The cholinergic regulation of potassium (86Rb+) permeability in sweat glands isolated from patients with cystic fibrosis.
abstract: Sweat glands isolated from skin obtained from normal subjects and patients with cystic fibrosis (CF) were pre-loaded with 86Rb+ and superfused with a physiological salt solution and the rate of 86Rb+ efflux was measured as an indicator of cellular potassium permeability. Acetylcholine always evoked a permeability increase in the glands from control subjects and this response could be resolved into calcium-dependent and calcium-independent components. Sweat glands from CF patients did not show such consistent responses. In three individuals the glands were abnormally insensitive to acetylcholine but normal responsiveness was seen in a fourth case. It is proposed that CF can induce dysfunction of calcium-dependent control processes in sweat glands.
authors.affiliation: Institute of Physiology, University of Glasgow.
authors.forename: S M
authors.initials: SM
authors.lastname: Wilson
authors.forename: J D
authors.initials: JD
authors.lastname: Pediani
authors.forename: F
authors.initials: F
authors.lastname: Cockburn
authors.forename: D L
authors.initials: DL
authors.lastname: Bovell
authors.forename: D M
authors.initials: DM
authors.lastname: Jenkinson
authors.forename: J Y
authors.initials: JY
authors.lastname: Paton
authors.forename: J
authors.initials: J
authors.lastname: Coutts
authors.forename: R
authors.initials: R
authors.lastname: Davidson
authors.forename: J
authors.initials: J
authors.lastname: Lambert
authors.forename: G
authors.initials: G
authors.lastname: Morris
mesh_terms: D000109:Acetylcholine / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D003550:Cystic Fibrosis / Q000378:metabolism*; D006801:Humans; D010539:Permeability / Q000187:drug effects; D011188:Potassium / Q000493:pharmacokinetics*; D012414:Rubidium Radioisotopes; D013545:Sweat Glands / Q000378:metabolism*
chemical_list: D012414:Rubidium Radioisotopes; D000109:Acetylcholine; D011188:Potassium; D002118:Calcium