title: An essential gene of Escherichia coli that has sequence similarity to a chloroplast gene of unknown function.
abstract: The dedB gene of Escherichia coli has sequence similarity to the zfpA gene of the chloroplast chromosome. The functions of dedB and zfpA are unknown. We constructed derivatives of temperature-sensitive polA strains into whose chromosomes a plasmid containing the disrupted dedB gene was integrated by homologous recombination. These strains contained normal and disrupted dedB genes in their chromosomes. We then selected plasmid-segregated strains and found no cells containing the disrupted dedB gene, indicating that disruption of the dedB gene was lethal in polA strains of E. coli.
authors.affiliation: Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nagano
authors.forename: R
authors.initials: R
authors.lastname: Matsuno
authors.forename: Y
authors.initials: Y
authors.lastname: Sasaki
mesh_terms: D015139:Blotting, Southern; D002470:Cell Survival / Q000235:genetics; D002736:Chloroplasts; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D010944:Plants / Q000235:genetics*; D010957:Plasmids