title: Effect of isometamidium on Trypanosoma congolense infectivity.
abstract: Isometamidium chloride (Samorin, RMB, England) is a widely used and highly effective trypanocide for the treatment of bovine trypanosomiases. However, the appearance of isometamidium-resistant populations of T. congolense in Africa makes it necessary to develop methods for the rapid and reliable detection of drug resistance in the laboratory. Currently available tests are time-consuming and/or expensive. In the present study, the short-term in vitro incubation of trypanosomes in a range of isometamidium concentrations and the infectivity of the parasites in mice has been assessed. A series of T. congolense isolates were used which were known to differ in their in vivo sensitivity to the drug. The results showed a close correlation between the known level of resistance and the capability of trypanosomes to remain infective after incubation in isometamidium. Thus isolates displaying a high level of resistance in vivo remained infective following incubation in higher concentrations of drug. This assay may provide a simple and reliable method for detecting drug resistance in T. congolense.
authors.affiliation: Department of Veterinary Physiology, University of Glasgow Veterinary School, UK.
authors.forename: I A
authors.initials: IA
authors.lastname: Sutherland
authors.forename: A
authors.initials: A
authors.lastname: Mounsey
authors.forename: P H
authors.initials: PH
authors.lastname: Holmes
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D051379:Mice; D010617:Phenanthridines / Q000494:pharmacology*; D014344:Trypanocidal Agents / Q000494:pharmacology*; D014348:Trypanosoma congolense / Q000187:drug effects* / Q000472:pathogenicity; D014353:Trypanosomiasis, African / Q000469:parasitology / Q000662:veterinary; D014354:Trypanosomiasis, Bovine / Q000469:parasitology*
chemical_list: D010617:Phenanthridines; D014344:Trypanocidal Agents; C000702:isometamidium chloride