UGA can be decoded as tryptophan at low efficiency in Bacillus subtilis.
Replacement of cat-86 codon 7 or 144 with the UGA codon permitted the gene to confer chloramphenicol resistance in wild-type Bacillus subtilis. UAA replacements of the same codons resulted in a chloramphenicol-sensitive phenotype in wild-type B. subtilis and a chloramphenicol-resistant phenotype in suppressor-positive strains. N-terminal sequencing showed that UGA at codon 7 was decoded as tryptophan in wild-type cells, at an efficiency of about 6%.
Lovett
P S
PS
Department of Biological Sciences, University of Maryland-Baltimore County, Catonsville 21228-5398.
Ambulos
N P
NP
Mulbry
W
W
Noguchi
N
N
Rogers
E J
EJ
D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D002702:Chloramphenicol Resistance / Q000235:genetics; D002874:Chromosome Mapping; D003062:Codon / Q000235:genetics*; D013489:Suppression, Genetic; D014169:Transformation, Bacterial; D014364:Tryptophan
D003062:Codon; D014364:Tryptophan