Metabolism of arachidonic acid in nervous system of marine mollusk Aplysia californica.
Studies of the marine mollusk Aplysia californica indicate that products of the 12-lipoxygenase pathway may be involved in neuronal intracellular signaling. The nervous tissue of Aplysia has a 12-lipoxygenase activity that converts both exogenous and endogenous arachidonic acid to an array of products, which include 12-hydroperoxyeicosatetraenoic acid (12-HPETE) and its metabolites hepoxilin A3, hepoxilin B3, 12-ketoeicosatetraenoic acid, and 12-oxododecatrienoic acid. These eicosanoids were identified using a combination of high-performance liquid chromatography, ultraviolet spectrometry and gas chromatography-mass spectrometry. Generation of 12-lipoxygenase products was stimulated by application of the neurotransmitters, histamine and FMRF-amide, or by stimulation of identified neural cells. In electrophysiological studies of identified L14 and sensory neurons it was found that 12-HPETE and its metabolic products exert physiological actions that resemble those of histamine and FMRF-amide. These results suggest that products of 12-HPETE metabolism may act as second messengers in Aplysia neurons.
Piomelli
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Laboratory of Molecular and Cellular Neurosciences, Rockefeller University, New York, New York 10021.
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D015126:8,11,14-Eicosatrienoic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D001048:Aplysia / Q000378:metabolism*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology; D006893:Hydroxyeicosatetraenoic Acids / Q000032:analysis; D009420:Nervous System / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism
C054994:12-keto-5,8,11,13-eicosatetraenoic acid; D001095:Arachidonic Acids; D005227:Fatty Acids; D006893:Hydroxyeicosatetraenoic Acids; D016718:Arachidonic Acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D006632:Histamine; C038243:8-hydroxy-11,12-epoxyeicosa-5,9,14-trienoic acid; D015126:8,11,14-Eicosatrienoic Acid