The genotoxicity of nitrilotriacetic acid (NTA) in a somatic mutation and recombination test in Drosophila melanogaster.
The genotoxicity of a chelating agent, the trisodium salt of nitrilotriacetic acid (NTA), was assessed in a somatic mutation and recombination test (SMART) in Drosophila melanogaster employing the wing hair markers mwh and flr3. The experiments were performed in parallel in two different laboratories (Padua, Italy and Schwerzenbach, Switzerland). The effectively absorbed doses of NTA, which was administered by feeding to larvae, were determined by a sensitive method employing [3H]leucine which allowed individual consumption levels to be measured. The particular pattern of clone induction produced by this compound suggests that NTA is active in inducing mitotic recombination and possibly aneuploidy in somatic cells of Drosophila. This is discussed in relation to the data present in the literature regarding the genotoxicity of NTA in a variety of experimental systems.
Zordan
M
M
Department of Biology, University of Padua, Italy.
Graf
U
U
Singer
D
D
Beltrame
C
C
Dalla Valle
L
L
Osti
M
M
Costa
R
R
Levis
A G
AG
D000818:Animals; D002614:Chelating Agents / Q000633:toxicity*; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics; D004435:Eating; D005787:Gene Frequency; D005819:Genetic Markers; D009152:Mutagenicity Tests; D009154:Mutation; D009571:Nitrilotriacetic Acid / Q000633:toxicity*; D012680:Sensitivity and Specificity
D002614:Chelating Agents; D005819:Genetic Markers; D009571:Nitrilotriacetic Acid