Long-term in vivo expression of retrovirus-mediated gene transfer in mouse fibroblast implants.
Toward the goal of gene therapy, we have been attempting to establish model somatic cell systems with the potential of sustained expression of the foreign gene. We report here that long-term expression of foreign genes in mouse embryo fibroblast implants can be achieved if a housekeeping gene promoter is used to drive transcription. Specifically, we have shown that in implants containing a beta-galactosidase gene linked to either an immediate early promoter of cytomegalovirus or a dihydrofolate reductase (DHFR) gene promoter, only the DHFR promoter allows long-term expression. We propose that choice of the promoter manifests significant influence on the long-term expression of genes introduced in fibroblast implants by retroviral vectors.
Scharfmann
R
R
Molecular Biology and Virology Laboratory, Salk Institute, San Diego, CA 92186-5800.
Axelrod
J H
JH
Verma
I M
IM
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004285:Dogs; D004622:Embryo, Mammalian; D005164:Factor IX / Q000235:genetics*; D005347:Fibroblasts / Q000201:enzymology / Q000637:transplantation; D015870:Gene Expression; D005822:Genetic Vectors; D006378:Helper Viruses / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012190:Retroviridae / Q000235:genetics*; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D005164:Factor IX; D001616:beta-Galactosidase