title: Electrochemical method for direct measurement of oxygen concentration and diffusivity in the intervertebral disc: electrochemical characterization and tissue-sensor interactions.
abstract: A method was developed for the in vivo measurement of oxygen concentration and diffusivity in the intervertebral disc using a bare gold cathode. The effects of surface adsorption were quantified using electrochemical methods and it was demonstrated that valid data can be obtained despite interactions between the electrode and the macromolecules of the intervertebral disc. The technique is used to show the dependence of the oxygen transport properties on tissue water content.
authors.affiliation: Physiological Flow Studies Unit, Imperial College, London.
authors.forename: D
authors.initials: D
authors.lastname: O'Hare
authors.forename: C P
authors.initials: CP
authors.lastname: Winlove
authors.forename: K H
authors.initials: KH
authors.lastname: Parker
mesh_terms: D000327:Adsorption; D001834:Body Water / Q000378:metabolism; D004058:Diffusion; D004553:Electric Conductivity; D004563:Electrochemistry / Q000379:methods*; D006046:Gold; D006801:Humans; D066298:In Vitro Techniques; D007403:Intervertebral Disc / Q000378:metabolism*; D008839:Microelectrodes; D010100:Oxygen / Q000378:metabolism*; D013499:Surface Properties
chemical_list: D006046:Gold; D010100:Oxygen