title: A DNA primer/probe system for the rapid and sensitive detection of Mycobacterium tuberculosis-complex pathogens.
abstract: A 1.5 kb EcoRI-BamHI restriction fragment from Mycobacterium tuberculosis was found to hybridize specifically with genomic DNA from M. tuberculosis-complex organisms. Primers were designed from the terminal sequences of this fragment and used to amplify uniquely M. tuberculosis-group DNA in a polymerase chain reaction. It is suggested that a combination of these primers and probe will prove a useful tool for the early diagnosis of tuberculous infections.
authors.affiliation: Department of Microbiology, University of Leicester, UK.
authors.forename: N T
authors.initials: NT
authors.lastname: Ralphs
authors.forename: S
authors.initials: S
authors.lastname: Garrett
authors.forename: R
authors.initials: R
authors.lastname: Morse
authors.forename: J B
authors.initials: JB
authors.lastname: Cookson
authors.forename: P W
authors.initials: PW
authors.lastname: Andrew
authors.forename: G J
authors.initials: GJ
authors.lastname: Boulnois
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics*; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI