title: Heterologously expressed serotonin 1A receptors couple to muscarinic K+ channels in heart.
abstract: In cardiac atrial cells, muscarinic acetylcholine receptors activate a K+ current directly via a guanine nucleotide-binding protein (G protein). Serotonin type 1A receptors may activate a similar pathway in hippocampal neurons. To develop a system in which receptor/G protein/K+ channel coupling can be experimentally manipulated, we have used a highly efficient recombinant vaccinia virus vector system to express human serotonin 1A receptors in primary cultures of rat atrial myocytes. The expressed 1A receptors activated the inwardly rectifying K+ conductance that is normally activated by the endogenous muscarinic acetylcholine receptors. Maximal responses to either agonist occluded further activation by the other agonist. The average activation time constants for serotonin were about 5 times slower than for acetylcholine. The data support suggestions that the intracellular signaling pathway from seven-helix receptors to G proteins and directly to ion channels is widespread in excitable cells. After a fraction of the G proteins are activated irreversibly by guanosine 5'-[gamma-thio]triphosphate, subsequent transduction proceeds more efficiently. One possible interpretation is that multiple G-protein molecules are required to activate each channel. Vaccinia virus expression vectors are thus useful for expressing seven-helix receptors in primary cultures of postmitotic cells and have provided a heterologous expression system for the signaling pathway from seven-helix receptors to G proteins and directly to ion channels.
authors.affiliation: Division of Biology 156-29, California Institute of Technology, Pasadena 91125.
authors.forename: A
authors.initials: A
authors.lastname: Karschin
authors.forename: B Y
authors.initials: BY
authors.lastname: Ho
authors.forename: C
authors.initials: C
authors.lastname: Labarca
authors.forename: O
authors.initials: O
authors.lastname: Elroy-Stein
authors.forename: B
authors.initials: B
authors.lastname: Moss
authors.forename: N
authors.initials: N
authors.lastname: Davidson
authors.forename: H A
authors.initials: HA
authors.lastname: Lester
mesh_terms: D000109:Acetylcholine / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004553:Electric Conductivity; D019204:GTP-Binding Proteins / Q000502:physiology; D066298:In Vitro Techniques; D008564:Membrane Potentials; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D015221:Potassium Channels / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D011940:Receptor Aggregation; D011976:Receptors, Muscarinic / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000378:metabolism*; D011994:Recombinant Proteins; D012701:Serotonin / Q000502:physiology; D013997:Time Factors; D014616:Vaccinia virus
chemical_list: D015221:Potassium Channels; D011976:Receptors, Muscarinic; D011985:Receptors, Serotonin; D011994:Recombinant Proteins; D012701:Serotonin; D019204:GTP-Binding Proteins; D000109:Acetylcholine