Formation of contacts between mast cells and sympathetic neurons in vitro.
Functional interactions between mast cells and peripheral nerves may occur at sites of association seen in vivo. To study the interactions, we developed a tissue culture model of murine sympathetic neurons co-cultured with rat basophilic leukaemia (RBL-2H3) cells (homologues of mucosal mast cells) or rat peritoneal mast cells. In co-cultures of up to 3 days, light microscopy identified neurite contacts with peritoneal mast cells or RBL-2H3 cells, but not with glial cells or fibroblasts. Electron microscopy confirmed membrane-membrane contact between neurites and RBL-2H3 cells. Time-lapse analysis of interactions between neurons and RBL-2H3 cells showed that 60-100% of the cells in a given field acquired neurite contact within 17 h. In matching control studies, there was no increase in the frequency of neurite contact with cells of the rat plasmacytoma line (YB2/0): these were not selected as targets, and contacts were broken if formed. Time-lapse records of the derivation of neurites from their path suggested a neurotropic effect of mast cells, with neurite contact ensuing when the intervening distance was less than 36 +/- 4 microns. Once formed, contacts were invariably maintained throughout the period of examination (up to 72 h), in contrast to YB2/O or fibroblast contacts. We conclude that neurons selectively form and maintain connections with cells representative of rat connective tissue-type and mucosal mast cells in vitro. Similar interactions in vivo could promote nerve/mast cell contacts, which may allow bidirectional communication between the nervous and immune systems.
Blennerhassett
M G
MG
Department of Pathology, McMaster University Health Sciences Center, Hamilton, Ontario, Canada.
Tomioka
M
M
Bienenstock
J
J
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D002450:Cell Communication / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D002478:Cells, Cultured; D005347:Fibroblasts / Q000473:pathology / Q000648:ultrastructure; D066298:In Vitro Techniques; D007942:Leukemia, Experimental / Q000473:pathology; D008407:Mast Cells / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D051379:Mice; D008808:Mice, Inbred CBA; D008854:Microscopy, Electron; D009474:Neurons / Q000502:physiology / Q000648:ultrastructure*; D010954:Plasmacytoma / Q000473:pathology; D051381:Rats; D013564:Sympathetic Nervous System / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D013997:Time Factors