title: The secretion genes of Pseudomonas aeruginosa alkaline protease are functionally related to those of Erwinia chrysanthemi proteases and Escherichia coli alpha-haemolysin.
abstract: The extracellular alkaline protease produced by Pseudomonas aeruginosa is secreted by a specific pathway, independent of the pathway used by most of the other extracellular proteins of this organism. Secretion of this protease is dependent on the presence of several genes located adjacent to the apr gene. Complementation studies have shown that PrtD, E, and F, the three secretion functions for Erwinia chrysanthemi proteases B and C (Létoffé et al., 1990), can mediate the secretion of the alkaline protease by Escherichia coli. The secretion functions involved in alpha-haemolysin secretion in E. coli (hlyB, hlyD, tolC) can also be used to complement alkaline protease secretion by E. coli, although less efficiently. These data indicate that protease secretion mechanisms in Pseudomonas and Erwinia are very similar and are homologous to that of E. coli alpha-haemolysin.
authors.affiliation: Unité de Génétique Moléculaire, Institut Pasteur, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Guzzo
authors.forename: F
authors.initials: F
authors.lastname: Duong
authors.forename: C
authors.initials: C
authors.lastname: Wandersman
authors.forename: M
authors.initials: M
authors.lastname: Murgier
authors.forename: A
authors.initials: A
authors.lastname: Lazdunski
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004885:Erwinia / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006460:Hemolysin Proteins; D015151:Immunoblotting; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*; D012697:Serine Endopeptidases / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D012697:Serine Endopeptidases; C032923:microbial serine proteinases