4-(N,N-dimethylaminosulphonyl)-7-fluoro-2,1,3-benzoxadiazole as chemilumigenic reagent for high performance liquid chromatographic peroxyoxalate chemiluminescence detection.
4-(N,N-Dimethylaminosulphonyl)-7-fluoro-2,1,3-benzoxadiazole (DBD-F), presented as a fluorogenic labelling reagent for amines and amino acids, is preferred for peroxyoxalate chemiluminescence (PO-CL) detection in high performance liquid chromatography. Amino acids and epinephrine derivatized with DBD-F were separated on a reversed phase column and detected at the femtomole level by the PO-CL detection system.
Uzu
S
S
Branch Hospital Pharmacy, University of Tokyo, Japan.
Imai
K
K
Nakashima
K
K
Akiyama
S
S
D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004837:Epinephrine / Q000032:analysis; D008163:Luminescent Measurements; D010070:Oxalates / Q000032:analysis*; D010080:Oxazoles; D013449:Sulfonamides
D000596:Amino Acids; D010070:Oxalates; D010080:Oxazoles; D013449:Sulfonamides; C044622:peroxyoxalate; C060548:4-(N,N-dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole; D004837:Epinephrine