Free radical-induced alterations of myocardial membrane proteins.
Rat myocardial membranes exposed to the free radical-generating systems, Fe2+/ascorbate, Cu2+/t-butylhydro-peroxide, linoleic acid hydroperoxide, and soybean lipoxygenase (Type I) undergo lipid peroxidation. This is evidenced by the accumulation of thiobarbituric acid-reactive substances and the loss of both extractable phospholipids and their polyunsaturated acyl groups. Lipid peroxidation is accompanied by alterations of membrane proteins including the general loss of polypeptides and accumulation of high-molecular weight material. The most sensitive protein is a polypeptide with a molecular weight of 28 kDa. At low levels of oxidation, this protein moves incrementally to slightly higher apparent molecular weight. At higher oxidant levels or longer periods of oxidation, the protein disappears completely from the SDS-PAGE gel. The "28K reaction" occurs prior to the massive, oxidant-induced lipid alterations and may thus indicate specific adduct formation between this protein and certain peroxidized membrane phospholipids.
Parinandi
N L
NL
Hormel Institute, University of Minnesota, Austin 55912.
Zwizinski
C W
CW
Schmid
H H
HH
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002413:Cations, Divalent; D003300:Copper / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology; D005609:Free Radicals; D006321:Heart / Q000187:drug effects*; D008041:Linoleic Acids / Q000494:pharmacology; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000494:pharmacology; D008084:Lipoxygenase / Q000494:pharmacology; D008563:Membrane Lipids / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D009206:Myocardium / Q000378:metabolism*; D010545:Peroxides / Q000494:pharmacology; D051381:Rats; D013025:Glycine max / Q000201:enzymology; D020122:tert-Butylhydroperoxide
D002413:Cations, Divalent; D005296:Ferrous Compounds; D005609:Free Radicals; D008041:Linoleic Acids; D008054:Lipid Peroxides; D008563:Membrane Lipids; D008565:Membrane Proteins; D010545:Peroxides; C008285:linoleic acid hydroperoxide; D003300:Copper; D020122:tert-Butylhydroperoxide; D008084:Lipoxygenase; D001205:Ascorbic Acid