title: The cortical actomyosin system of cytochalasin D-treated lymphoblasts.
abstract: Global cytoskeleton dynamics is likely to exist in animal cells and some experimental evidence for this has recently been obtained in cells from the human lymphoblastic cell line KE37. We have further investigated the dramatic and reversible microtubule-dependent cell elongation which occurs upon treatment of KE37 cells with cytochalasin D. This phenomenon results in a non-locomotory cell with definite polarity. It involves a sustained equatorial myosin II-dependent contraction of cortical, most of the myosin II being accumulated on segments of the main cellular extension. We report here that such a cell lengthening is energy-dependent and can be inhibited, or suppressed, by surface ligands such as wheat germ agglutinin but not by concanavalin A. Suppression of the cytochalasin D effect by wheat germ agglutinin is rapid and appears to be collapse of the cell extension and relocalization of the contracted actomyosin as a whole. It suggests that the binding of the wheat germ agglutinin to the cell surface results in the transient disassembly of microtubules, a possibility also raised by the potent antagonist effect of taxol on wheat germ agglutinin action. Taken together, the data are consistent with a specific role of microtubules in the control of the activity of the cortical actomyosin system.
authors.affiliation: Centre de Genetique Moléculaire, CNRS, Gif-sur-Yvette, France.
authors.forename: E
authors.initials: E
authors.lastname: Bailly
authors.forename: C
authors.initials: C
authors.lastname: Celati
authors.forename: M
authors.initials: M
authors.lastname: Bornens
mesh_terms: D000205:Actomyosin / Q000502:physiology*; D001386:Azides / Q000494:pharmacology; D002460:Cell Line; D015638:Cytochalasin D / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D015739:Nocodazole / Q000494:pharmacology; D019810:Sodium Azide; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects; D014909:Wheat Germ Agglutinins / Q000494:pharmacology
chemical_list: D001386:Azides; D014909:Wheat Germ Agglutinins; D015638:Cytochalasin D; D000205:Actomyosin; D019810:Sodium Azide; D015739:Nocodazole