title: Glucan structure in a fragile mutant of Saccharomyces cerevisiae.
abstract: The phenotype of VY1160 fragile Saccharomyces cerevisiae mutant is characterized by cell lysis upon transfer to hypotonic solutions and increased permeability of cells growing in osmotically stabilized media. Two mutations, srb1 and ts1, have been identified in VY1160 cells and previous studies have shown that the increased permeability is due to the ts1 mutation which causes a shortening of mannan side-chains. Here we report that the srb1 mutation, which is the genetic determinant of cell lysis, is responsible for quantitative and structural changes of glucans. Experiments with isogenic single mutation strains, genetic studies coupled with quantitative measurements of glucan content per cell, and methylation analysis of glucans provide evidence that srb1 mutation leads to i) formation of mechanically unstable cell wall network made of insoluble glucan fibrils which are shorter and contain beta(1-6) inter-residue linkages and ii) insufficient filling of the space between the fibrils due to a shortage of the alkali-soluble glucan. Although growing exponentially in osmotically stabilized media, the srb1 cells cannot resist an osmotic shock and, hence, burst immediately.
authors.affiliation: Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
authors.forename: J
authors.initials: J
authors.lastname: Blagoeva
authors.forename: G
authors.initials: G
authors.lastname: Stoev
authors.forename: P
authors.initials: P
authors.lastname: Venkov
mesh_terms: D002849:Chromatography, Gas; D002850:Chromatography, Gel; D005936:Glucans / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D008745:Methylation; D008970:Molecular Weight; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000032:analysis* / Q000235:genetics
chemical_list: D005936:Glucans