[Study of oligohemoglobin solutions in vitro and in vivo].
Solutions of oligohemoglobins (OHb) with middle molecular mass 100 x 10(3) = 400 x 10(3), obtained after polycondensation of hemoglobin with glutaric aldehyde as well as oligohemoglobin modified with pyridoxal-5-phosphate, were studied in vivo and in vitro (plethoric administration, isovolemic metabolic substitution, hemorrhagic shock; at a dose of 0.5-1.8 g/kg of body mass; dogs, rabbits, rats). With increase of the OHb molecular mass period of its circulation in blood was elevated; blood plasma protected OHb from autooxidation; half-life of OHb was about 12-18 hrs in isovolemic metabolic substitution and in hemorrhagic shock and about 5 hrs--in plethoric administration. During circulation molecular mass selection of OHb occurred, where low molecular fraction decreased and high molecular fraction--accumulated; sign and value of human erythrocyte charge were similar both in OHb solutions with concentration up to 5% and in physiological solution.
Kuznetsova
N P
NP
Gudkin
L R
LR
Mishaeva
R N
RN
Stragovich
L M
LM
Samsonov
G V
GV
Selivanov
E A
EA
Bystrova
I M
IM
Gerbut
K A
KA
Molokovskaia
I E
IE
Kochetygov
N I
NI
D000818:Animals; D004285:Dogs; D004912:Erythrocytes / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011732:Pyridoxal Phosphate / Q000737:chemistry; D012771:Shock, Hemorrhagic / Q000097:blood; D012996:Solutions
D006454:Hemoglobins; D012996:Solutions; D011732:Pyridoxal Phosphate; D005976:Glutaral
Issledovanie rastvorov oligogemoglobina in vitro i in vivo.