title: Antioxidants stimulate endothelial cell proliferation in culture.
abstract: The effect of antioxidants on the proliferation of cultured endothelial cells (ECs) were studied. Probucol, a lipid-lowering drug with antioxidant properties, added to the growth medium stimulated the proliferation of ECs. D1-alpha-tocopherol (tocopherol), a natural antioxidant, and butylated hydroxytoluene, a synthetic antioxidant also had the same effect on the proliferation of ECs. There was no significant difference in the content of thiobarbituric acid-reacting substances between the probucol-containing growth medium and control medium during the growth assay. Pretreatment of ECs with probucol or tocopherol also enhanced ECs proliferation in medium without any added antioxidants. The addition of free radical scavengers superoxide dismutase, catalase and mannitol to the growth medium did not enhance the proliferation of ECs. These findings indicate that the stimulatory effect on ECs proliferation of antioxidants in the growth medium appears to be unrelated to preventing free radical reactions in the medium itself, but rather may be related to the uptake of antioxidants by ECs.
authors.affiliation: Department of Geriatrics, Nagoya University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kuzuya
authors.forename: M
authors.initials: M
authors.lastname: Naito
authors.forename: C
authors.initials: C
authors.lastname: Funaki
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
authors.forename: K
authors.initials: K
authors.lastname: Yamada
authors.forename: K
authors.initials: K
authors.lastname: Asai
authors.forename: F
authors.initials: F
authors.lastname: Kuzuya
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001685:Biological Factors / Q000032:analysis; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002374:Catalase / Q000494:pharmacology; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D003470:Culture Media / Q000032:analysis; D004730:Endothelium, Vascular / Q000187:drug effects*; D008353:Mannitol / Q000494:pharmacology; D011341:Probucol / Q000378:metabolism / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology; D013858:Thiobarbiturates / Q000032:analysis; D014810:Vitamin E / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D001685:Biological Factors; D003470:Culture Media; D013858:Thiobarbiturates; D014810:Vitamin E; D002084:Butylated Hydroxytoluene; D008353:Mannitol; D002374:Catalase; D013482:Superoxide Dismutase; D011341:Probucol