title: Uptake of extracellular Ca2+ is a requirement for production of interferon-gamma by cord blood mononuclear cells.
abstract: We studied the response of adult peripheral and cord blood mononuclear cells (MNCs) to graded concentrations of phytohemagglutinin (PHA) to characterize the requirements for proliferation and interferon-gamma (IFN-gamma) production. As the concentration of PHA was decreased, the proliferative response of both adult and cord blood MNCs decreased in parallel. At high concentrations of PHA (a: 120) cord blood MNCs display normal high proliferation but IFN-gamma production was greatly diminished compared with adult MNCs. Addition of the calcium ionophore, A23187, to PHA-stimulated adult MNCs had no effect on proliferation or IFN-gamma production at any of the concentrations of PHA tested. Furthermore, A23187 alone had no effect on proliferation or IFN-gamma secretion. Similarly, the addition of A23187 to PHA-stimulated cord-blood MNCs had no effect on proliferation. However, when A23187 was added to high dose (1:120) PHA-stimulated cord blood MNCs IFN-gamma production increased to levels comparable with adult PHA-stimulated MNCs. A similar pattern of response was seen when exogenous calcium chloride was added in place of A23187.
authors.affiliation: Department of Pediatrics, UCLA School of Medicine 90024-1745.
authors.forename: A M
authors.initials: AM
authors.lastname: Kesson
authors.forename: Y J
authors.initials: YJ
authors.lastname: Bryson
mesh_terms: D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000097:blood*; D002122:Calcium Chloride / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology
chemical_list: D010835:Phytohemagglutinins; D000001:Calcimycin; D007371:Interferon-gamma; D002122:Calcium Chloride; D002118:Calcium