Phototoxicity induced by nalidixic and oxolinic acids: decrease in cell survival of chick embryo fibroblasts and Hep-2 cells.
The phototoxic effects of nalidixic and oxolinic acids were evaluated in two types of cultured cells: chick embryo fibroblast and Hep-2 (human laryngo carcinoma cell line). In order to evaluate the phototoxicity induced by nalidixic and oxolinic acids, both cell types were irradiated for 5 min in the presence of each drug. The results showed an inverse relationship between cell survival and the concentration of the drug added to the culture medium. The concentrations of nalidixic and oxolinic acids necessary to induce a phototoxic effect were in the range of therapeutic blood levels. Both chick embryo fibroblasts and Hep-2 cells were more sensitive to the phototoxic effect induced by nalidixic acid than oxolinic acid.
Cortés
M P
MP
Laboratorio de Fotobiología y Fotoquímica, Facultad de Medicina, Universidad de Valparaíso, Chile.
Cárdenas
A M
AM
Navarrete
E
E
Fernández
E
E
D000818:Animals; D002277:Carcinoma / Q000473:pathology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002642:Chick Embryo; D005347:Fibroblasts / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D008027:Light; D009268:Nalidixic Acid / Q000633:toxicity*; D010093:Oxolinic Acid / Q000633:toxicity*; D014407:Tumor Cells, Cultured
D009268:Nalidixic Acid; D010093:Oxolinic Acid