Reaction tray and noncontact transfer method for heterogeneous chemiluminescence immunoassays.
We describe a reaction tray for a heterogeneous chemiluminescence (CL) immunoassay having the following features: separate sample incubation and signal detection wells; a design that allows for noncontact transfer of the reaction mixture from incubation wells to detection wells; surface features to mate with a detector and create a light-tight seal for CL detection; and self-contained means for liquid removal. The reaction mixture is transferred by injecting a wash solution from a group of nozzles into the incubation well. Quantitative transfer of microparticles (transfer efficiencies greater than 95% and CV less than 5%) is achieved by injecting two 300-microL pulses of transfer solution at a rate of 2.1 m/s. The performance of the tray and method of transfer is tested by determining the precision of CL signal for a sample containing a concentration of anti-hepatitis B core antigen (anti-HBc) or hepatitis B surface antigen (HBsAg) close to the cutoff value for the assay.
Khalil
O S
OS
Diagnostics Division, Abbott Laboratories, Abbott Park, IL 60064.
Hanna
C F
CF
Huff
D
D
Zurek
T F
TF
Murphy
B
B
Pepe
C
C
Genger
K
K
D001667:Binding, Competitive; D006512:Hepatitis B Core Antigens / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D008163:Luminescent Measurements
D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens