title: Genetic recombination in Streptomyces michiganensis DSM 40,015 revealed three genes responsible for the formation of melanin.
abstract: By UV and NTG mutagenesis numerous auxotrophic, antibiotic-resistant and melanin-negative mutants were isolated from Streptomyces michiganensis DSM 40,015 which proved to possess a efficient photoreactivation system. Using a mel- test strain with three auxotrophic markers and a antibiotic resistance for crosses with numerous prototrophic mel- strains three classes of mutants (melA, melB and melC) could be found. This classification was further supported by a series of crosses. The melC locus seemed to correspond to the melC locus of S. glaucescens which contains the tyrosinase structural gene.
authors.affiliation: Institut für Mikrobiologie, Technische Hochschule Darmstadt, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Held
authors.forename: H J
authors.initials: HJ
authors.lastname: Kutzner
mesh_terms: D002874:Chromosome Mapping; D005798:Genes, Bacterial; D008543:Melanins / Q000096:biosynthesis*; D014442:Monophenol Monooxygenase / Q000096:biosynthesis; D009154:Mutation; D011995:Recombination, Genetic; D013302:Streptomyces / Q000235:genetics*
chemical_list: D008543:Melanins; D014442:Monophenol Monooxygenase