Plasma neutrophil-activating peptide-1/interleukin-8 and neutrophil elastase in a primate bacteremia model.
A hyperdynamic sepsis model was set up in seven adult baboons to evaluate neutrophil-activating peptide-1/interleukin (IL)-8 (NAP-1/IL-8), IL-1 beta, IL-6, tumor necrosis factor-alpha (TNF alpha), and IFN-gamma in plasma. By continuous intravenous administration of 10(10) cfu/kg live Escherichia coli over 8 h with additional infusion therapy (less than or equal to 50 ml/kg/h), endotoxin plasma levels of 2.7-22.3 ng/ml were observed. In plasma the kinetics of NAP-1/IL-8 and IL-6 were similar to those of IL-1 at the end of the experiment (8 h) (peak median values, 34, 4197, and 230 ng/ml, respectively). Differences were greatest for IL-6. Monocyte activation during sepsis was confirmed by elevated plasma neopterin levels (91-139 mumol/mmol of creatine). Granulocyte activation was evident from both incipient neutropenia and the massive release of neutrophil elastase into the plasma as measured by a new immunoassay (peak level, 374 ng/ml). Thus, in primate bacteremia, early TNF release is followed by a concomitant increase of NAP-1/IL-8 with plasma kinetics similar to those of IL-6 and IL-1 and accompanied by massive activation of neutrophils.
Redl
H
H
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria.
Schlag
G
G
Bahrami
S
S
Schade
U
U
Ceska
M
M
Stütz
P
P
D000818:Animals; D001708:Biopterins / Q000031:analogs & derivatives / Q000097:blood; D004195:Disease Models, Animal; D004731:Endotoxins / Q000097:blood; D004927:Escherichia coli Infections / Q000097:blood* / Q000201:enzymology; D007371:Interferon-gamma / Q000097:blood; D007375:Interleukin-1 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood*; D019272:Leukocyte Elastase; D008297:Male; D019798:Neopterin; D010196:Pancreatic Elastase / Q000097:blood*; D010215:Papio; D018805:Sepsis / Q000097:blood* / Q000201:enzymology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D004731:Endotoxins; D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D001708:Biopterins; D019798:Neopterin; D007371:Interferon-gamma; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase