title: Identification and sequence analysis of the hupR1 gene, which encodes a response regulator of the NtrC family required for hydrogenase expression in Rhodobacter capsulatus.
abstract: The hupR1 gene from Rhodobacter capsulatus was cloned and sequenced. It can encode a protein of 53,843 Da which shares significant similarity with several transcriptional regulators and activates transcription of the structural hupSL genes of [NiFe]hydrogenase, as shown by the use of a translational fusion of lacZ with the hupSL promoter. A Hup- mutant having a point mutation in the hupR1 gene is described.
authors.affiliation: Laboratoire de Biochimie Microbienne (Centre National de la Recherche Scientifique Unité 1130 alliée à l'Institut National de la Santé de la Recherche Médicale), Grenoble, France.
authors.forename: P
authors.initials: P
authors.lastname: Richaud
authors.forename: A
authors.initials: A
authors.lastname: Colbeau
authors.forename: B
authors.initials: B
authors.lastname: Toussaint
authors.forename: P M
authors.initials: PM
authors.lastname: Vignais
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006864:Hydrogenase / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D015183:Restriction Mapping; D016354:Rhodobacter capsulatus / Q000235:genetics*; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D014157:Transcription Factors; C070743:hupR1 protein, Rhodobacter capsulatus; D006864:Hydrogenase