[Gangliosides modulate lipoxygenase oxidation in human lymphocytes].
Using reverse phase high performance chromatography with UV-detection, the arachidonic acid cascade in human peripheral blood lymphocytes (PBL) was studied. It was found that PBL oxidized arachidonic acid via the lipoxygenase pathway, 12-hydroxyeicosatetraenoic acid (12-HETE) being the major metabolite of endogenous arachidonic acid. Exogenous arachidonic acid added to human PBL suspensions increased 12-HETE synthesis 5-7 times. In another experimental series the effects of gangliosides (GD3, GM1 and GM3) on lipoxygenase-catalyzed oxidation of arachidonic acid in human lymphocytes were investigated. All the gangliosides tested stimulated PBL to secrete 12-HETE both from endogenous and exogenous arachidonic acid. In most cases the stimulating effect of GD3 was much more apparent that those of GM1 and GM3.
Bezuglov
V V
VV
Fomina-Ageeva
E V
EV
Gretskaia
N M
NM
Kriukova
E V
EV
Diatlovitskaia
E V
EV
Bergel'son
L D
LD
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005732:Gangliosides / Q000378:metabolism*; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000276:immunology; D008084:Lipoxygenase / Q000378:metabolism*; D008214:Lymphocytes / Q000201:enzymology*; D010084:Oxidation-Reduction; D013056:Spectrophotometry, Ultraviolet
D001095:Arachidonic Acids; D005732:Gangliosides; D006893:Hydroxyeicosatetraenoic Acids; D016718:Arachidonic Acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D008084:Lipoxygenase
Gangliozidy moduliruiut lipoxsigenaznoe okislenie v limfotsitakh cheloveka.