title: Contribution of the B oligomer to the protective activity of genetically attenuated pertussis toxin.
abstract: An enzymatically deficient recombinant S1 subunit, in which Arg-9 was replaced by Lys, was combined with native B oligomer to form a mutant holotoxin molecule. This molecule exhibited decreased leukocytosis-promoting and histamine-sensitizing activities compared with those of the native toxin, supporting the view that the B oligomer is not responsible for these activities. The protective activity of this genetically attenuated pertussis toxin was compared with that of B oligomer alone. The mutant pertussis toxin and B oligomer were similarly capable of protecting mice against a respiratory infection with Bordetella pertussis, suggesting that the B oligomer makes a significant contribution to the protection afforded by the genetically attenuated holotoxin.
authors.affiliation: Division of Bacterial Products, Center for Biologics Evaluation and Research, Bethesda, Maryland 20892.
authors.forename: J L
authors.initials: JL
authors.lastname: Arciniega
authors.forename: R D
authors.initials: RD
authors.lastname: Shahin
authors.forename: W N
authors.initials: WN
authors.lastname: Burnette
authors.forename: T D
authors.initials: TD
authors.lastname: Bartley
authors.forename: D W
authors.initials: DW
authors.lastname: Whiteley
authors.forename: V L
authors.initials: VL
authors.lastname: Mar
authors.forename: D L
authors.initials: DL
authors.lastname: Burns
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D006224:Cricetinae; D005260:Female; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D037342:Pertussis Toxin; D010567:Pertussis Vaccine / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*; D010566:Virulence Factors, Bordetella / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D010567:Pertussis Vaccine; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin