title: Isotachophoretic determination of 2-5A phosphodiesterase.
abstract: Together with 2-5A synthetase and ribonuclease L, 2-5A phosphodiesterase belongs to the 2-5A system, which plays an important role in the action of interferon. Analytical capillary isotachophoresis was used for the determination of 2-5A phosphodiesterase activity. Enzyme assay was optimized using snake venom phosphodiesterase as a source of 2-5A phosphodiesterase activity. The 2-5A trimer core was used as a substrate. Enzyme activity was determined in time- and concentration-dependent reactions. In addition, 2-5A phosphodiesterase activity was determined in lysates of mononuclear blood cells.
authors.affiliation: Children's Hospital, University of Tübingen, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Bruchelt
authors.forename: M
authors.initials: M
authors.lastname: Buedenbender
authors.forename: K H
authors.initials: KH
authors.lastname: Schmidt
authors.forename: B
authors.initials: B
authors.lastname: Jopski
authors.forename: J
authors.initials: J
authors.lastname: Treuner
authors.forename: D
authors.initials: D
authors.lastname: Niethammer
mesh_terms: D000227:Adenine Nucleotides / Q000737:chemistry / Q000378:metabolism; D004586:Electrophoresis; D005095:Exoribonucleases / Q000378:metabolism*; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000201:enzymology; D015394:Molecular Structure; D009843:Oligoribonucleotides / Q000737:chemistry / Q000378:metabolism
chemical_list: D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; C023505:2',5'-oligoadenylate; D005095:Exoribonucleases; C029364:2'-phosphodiesterase