Accumulation of a poly(hydroxyalkanoate) copolymer containing primarily 3-hydroxyvalerate from simple carbohydrate substrates by Rhodococcus sp. NCIMB 40126.
A number of taxonomically-related bacteria have been identified which accumulate poly(hydroxyalkanoate) (PHA) copolymers containing primarily 3-hydroxyvalerate (3HV) monomer units from a range of unrelated single carbon sources. One of these, Rhodococcus sp. NCIMB 40126, was further investigated and shown to produce a copolymer containing 75 mol% 3HV and 25 mol% 3-hydroxybutyrate (3HB) from glucose as sole carbon source. Polyesters containing both 3HV and 3HB monomer units, together with 4-hydroxybutyrate (4HB), 5-hydroxyvalerate (5HV) or 3-hydroxyhexanoate (3HHx), were also produced by this organism from certain accumulation substrates. With valeric acid as substrate, almost pure (99 mol% 3HV) poly(3-hydroxyvalerate) was produced. N.m.r. analysis confirmed the composition of these polyesters. The thermal properties and molecular weight of the copolymer produced from glucose were comparable to those of PHB produced by Alcaligenes eutrophus.
Haywood
G W
GW
Department of Applied Biology, University of Hull, UK.
Anderson
A J
AJ
Williams
D R
DR
Dawes
E A
EA
Ewing
D F
DF
D005947:Glucose / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D010421:Pentanoic Acids / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D012240:Rhodococcus / Q000378:metabolism*; D013816:Thermodynamics; D000094122:Polyhydroxybutyrates
D005947:Glucose; D010421:Pentanoic Acids; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C013056:beta-hydroxyvaleric acid