title: Characterization of a novel erythropoiesis-inhibiting human protein.
abstract: We have isolated an erythropoiesis-inhibiting protein, DIP (differentiation-inhibiting protein), from the blood of a 60-year-old woman suffering from pure red cell aplasia. This protein inhibits the growth and differentiation of normal human and murine BFU-E, but not CFU-E, cells as well as dimethyl sulfoxide-induced hemoglobin synthesis by Friend murine erythroleukemia cells. It appears that DIP primarily affects differentiation rather than proliferation, because it does not inhibit the proliferation of untreated Friend erythroleukemia cells. DIP seems to function like a recently described 45-kDa autocrine differentiation-inhibiting protein factor (ADIF) which is secreted by tsAEV-transformed chicken erythroleukemia cells. Both proteins selectively block the differentiation of normal human and murine BFU-E cells as well as the differentiation (but not the proliferation) of Friend murine erythroleukemia cells. However, the human DIP is not an autocrine product of the patient's bone marrow cells, nor does it affect chicken erythroid cells.
authors.affiliation: Institute for Biological Sciences, National Research Council of Canada, Ottawa.
authors.forename: J P
authors.initials: JP
authors.lastname: Durkin
authors.forename: J M
authors.initials: JM
authors.lastname: Biquard
authors.forename: J P
authors.initials: JP
authors.lastname: Blanchet
authors.forename: J F
authors.initials: JF
authors.lastname: Whitfield
authors.forename: R
authors.initials: R
authors.lastname: Doyonnas
authors.forename: P
authors.initials: P
authors.lastname: MacDonald
authors.forename: N
authors.initials: N
authors.lastname: Morardet
authors.forename: J
authors.initials: J
authors.lastname: Royer
authors.forename: R
authors.initials: R
authors.lastname: Tremblay
authors.forename: V
authors.initials: V
authors.lastname: Krsmanovic
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002645:Chickens; D004920:Erythropoiesis / Q000187:drug effects; D005260:Female; D006131:Growth Inhibitors / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D051379:Mice; D008875:Middle Aged; D012010:Red-Cell Aplasia, Pure / Q000097:blood
chemical_list: D001798:Blood Proteins; D006131:Growth Inhibitors; C007627:erythropoiesis inhibiting factors