Isolation and chemical study of the glycosaminoglycans from squid cornea.
1. Oversulphated chondroitin sulphate (ca 93% of tissue glycosaminoglycans) with average molecular weight 72,500, chondroitin sulphate (5%) and small amounts of lowsulphated chondroitin sulphate were isolated from squid cornea. 2. The sulphation pattern of oversulphated chondroitin sulphate was delta di-4S (52%), delta di-diSD (28%), delta di-6S (9%) and delta di-OSCS (11%) and that of chondroitin sulphate 49, 1, 20 and 30% respectively. 3. All glycosaminoglycans contained neutral monosaccharides, glucose being the predominant neutral monosaccharide in oversulphated chondroitin sulphate and chondroitin sulphate and fucose in low-sulphated chondroitin sulphate. 4. Although L-iduronic acid was not detected, the digestion of oversulphated chondroitin sulphate with chondroitinases ABC and AC gave unexpected results.
Karamanos
N K
NK
Department of Chemistry, University of Patras, Greece.
Manouras
A
A
Tsegenidis
T
T
Antonopoulos
C A
CA
D000818:Animals; D002809:Chondroitin Sulfates / Q000032:analysis* / Q000302:isolation & purification; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D003315:Cornea / Q000737:chemistry; D049832:Decapodiformes / Q000032:analysis*; D006025:Glycosaminoglycans / Q000032:analysis* / Q000302:isolation & purification; D009005:Monosaccharides / Q000032:analysis
D006025:Glycosaminoglycans; D009005:Monosaccharides; D002809:Chondroitin Sulfates