Effect of chronic exposure of PCB (Aroclor 1254) on specific and nonspecific immune parameters in the rhesus (Macaca mulatta) monkey.
The immunomodulatory effects of low-level, chronic polychlorinated biphenyl PCB; (Aroclor 1254) exposure were investigated in female rhesus (Macaca mulatta) monkeys. Five groups of monkeys (initially 16 monkeys/group) were orally administered PCB at levels of 0, 5, 20, 40 or 80 micrograms/kg body wt/day. Tests for immunomodulation were initiated after 55 months of exposure to PCBs. Statistically significant observed immune changes included a dose-related decrease in the anamnestic (IgM and IgG) response to sheep red blood cells. Conversely, the antibody response to pneumococcus antigen did not differ significantly across the test groups. A statistically significant dose-related decrease in lymphoproliferation was noted with increasing doses of PCBs when phytohemagglutinin and Concanavalin A, but not when pokeweed mitogen, were used as mitogens. A trend toward reduced peak chemiluminescence (mV/min) was observed in zymosan-activated peripheral blood monocytes. The time to peak chemiluminescence of phorbol myristate acetate activation was statistically increased in a dose-response fashion. Flow cytometric analysis results of peripheral blood lymphocytes using the markers CD4, CD8, and CD20 were similar across the test groups. The mean percentage levels for the CD2 marker in the treated groups were statistically lower than the mean in the control, while absolute numbers for CD2 were similar across the test groups. Serum hydrocortisone levels did not differ among the test groups. Taken together these results indicate that low-level, chronic PCB exposure alters a number of rhesus monkey immune system components and that these effects may be due to altered T-cell and/or macrophage function. These data may be of use in extrapolating potential human health effects following chronic PCB exposure.
Tryphonas
H
H
Toxicology Research Division, Health and Welfare, Ottawa, Ontario, Canada.
Luster
M I
MI
Schiffman
G
G
Dawson
L L
LL
Hodgen
M
M
Germolec
D
D
Hayward
S
S
Bryce
F
F
Loo
J C
JC
Mandy
F
F
D000818:Animals; D001140:Aroclors / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D020111:Chlorodiphenyl (54% Chlorine); D004912:Erythrocytes / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006854:Hydrocortisone / Q000097:blood; D007109:Immunity / Q000187:drug effects*; D007375:Interleukin-1 / Q000096:biosynthesis; D008163:Luminescent Measurements; D008214:Lymphocytes / Q000187:drug effects; D008253:Macaca mulatta; D009000:Monocytes / Q000187:drug effects; D012756:Sheep; D013296:Streptococcus pneumoniae / Q000276:immunology
D001140:Aroclors; D007375:Interleukin-1; D020111:Chlorodiphenyl (54% Chlorine); D006854:Hydrocortisone