Some taxonomical characteristics of encapsulated Lactobacillus sp. KPB-167B isolated from kefir grains and characterization of its extracellular polysaccharide.
A capsular polysaccharide-producing strain KPB-167B isolated from kefir grains was identified as a homofermentative Lactobacillus. The carbohydrate fermentation pattern and DNA base composition of the strain were different from those of other capsular Lactobacillus species previously isolated from kefir grains. The polysaccharide produced by Lactobacillus sp. KPB-167B was found similar to kefiran by 13C-NMR and methylation analysis. Lactobacillus sp. KPB-167B could grow and produce capsular polysaccharide in MRSL medium with better yield than L. kefiranofaciens, which suggested that it is suitable for kefiran production.
Yokoi
H
H
Hiratsuka Research Laboratory, Sumitomo Heavy Industries, Ltd., Kanagawa, Japan.
Watanabe
T
T
Fujii
Y
Y
Mukai
T
T
Toba
T
T
Adachi
S
S
D000818:Animals; D001482:Base Composition; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004269:DNA, Bacterial / Q000032:analysis; D005516:Food Microbiology; D007778:Lactobacillus / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008892:Milk / Q000382:microbiology*; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000737:chemistry*
D004269:DNA, Bacterial; D011135:Polysaccharides, Bacterial