Duodenal rapeseed oil infusion in early and midlactation cows. 4. In vivo and in vitro adipose tissue lipolytic responses.
In vitro glycerol and FFA releases from adipose tissue were studied in early (wk 3, trial 1) and midlactation (wk 19 to 26, trial 2) multiparous Holstein Friesian cows receiving a duodenal rapeseed oil infusion (1.0 to 1.1 kg/d). In trial 2, in vitro basal FFA release, basal FFA: glycerol ratio, and isoproterenol-stimulated FFA and glycerol releases were higher in perirenal adipose tissue from oil-infused cows. Plasma FFA concentration also was higher in oil-infused cows before and after intravenous isoproterenol injection. In trail 1, basal and stimulated glycerol and FFA releases from perirenal (but not subcutaneous) adipose tissue tended to be lower in oil-infused cows. This was probably linked to a lower milk production potential of oil-infused than of control cows which introduced a bias in energy balance. The basal FFA:glycerol ratio tended to be higher in oil-infused cows in both adipose tissues, suggesting a lower rate of reesterification inducted by oil, as was the case in trial 2. The alpha 2-agonist clonidine decreased perirenal adipose tissue glycerol release in cows and treatments in which the responses to 4 x 10(-7) M isoproterenol were higher. In vivo and in vitro lipolytic responses were lower in trial 2 than in trial 1, except the in vitro maximally stimulated lipolytic rate, which probably reflected a long lasting teleophoretic adaptation to ensure energy needs of lactation. This study indicated that oil infusion affected both beta- and alpha 2-adrenergic responses and that postpartum lipid mobilization did not seem to be reduced by the exogenous unsaturated fatty acid supply.
Gagliostro
G
G
Laboratoire de la Lactation, Institut National de la Recherche Agronomique, Saint Genès Champanelle, France.
Chilliard
Y
Y
D000243:Adenosine Deaminase / Q000494:pharmacology; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001937:Brassica; D002417:Cattle / Q000502:physiology*; D003000:Clonidine / Q000494:pharmacology; D004386:Duodenum / Q000378:metabolism*; D004734:Energy Metabolism; D005229:Fatty Acids, Monounsaturated; D005230:Fatty Acids, Nonesterified / Q000097:blood / Q000378:metabolism; D005260:Female; D005990:Glycerol / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology; D007774:Lactation / Q000502:physiology*; D008066:Lipolysis; D010938:Plant Oils / Q000008:administration & dosage*; D000074262:Rapeseed Oil; D013806:Theophylline / Q000494:pharmacology
D005229:Fatty Acids, Monounsaturated; D005230:Fatty Acids, Nonesterified; D010938:Plant Oils; D000074262:Rapeseed Oil; D013806:Theophylline; D000243:Adenosine Deaminase; D007545:Isoproterenol; D003000:Clonidine; D005990:Glycerol