Selection of sequences recognized by a DNA binding protein using a preparative southwestern blot.
We have tested the feasibility of using a preparative scale Southwestern blot to select DNA sequences specifically recognized by a DNA binding protein. We constructed a library of random 15-mer oligonucleotides and screened it using a preparative Southwestern blot with the transcriptional repressor, PRDI-BF1. Several sequences specifically recognized by PRDI-BF1 were isolated. All of these sequences are similar to PRDI, a regulatory element previously demonstrated to bind PRDI-BF1.
Keller
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AD
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
Maniatis
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T
D001483:Base Sequence; D015342:DNA Probes / Q000235:genetics / Q000378:metabolism; D007370:Interferon Type I / Q000235:genetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors
D015342:DNA Probes; D007370:Interferon Type I; D009838:Oligodeoxyribonucleotides; D012097:Repressor Proteins; D014157:Transcription Factors