Isolation and characterization of a cDNA clone encoding the anti-viral protein from Phytolacca americana.
Phytolacca anti-viral protein (PAP) was purified from Phytolacca leaves and the N-terminal was sequenced. A cDNA library was made from mRNAs isolated from Phytolacca leaves and cDNA clones for PAP were identified using oligonucleotide probes derived from the N-terminal amino acid sequence. The PAP-cDNA clone was sequenced from both directions. The predicted amino acid sequence of PAP was compared with the amino acid sequences of other ribosome-inactivating proteins. The identities of these proteins to PAP ranged from 29 to 38%, and a region was found in each with a sequence similar to the PAP sequence (AIQMVSEAARFKYI). Southern blot analysis indicates that PAP is encoded by a multi-gene family.
Lin
Q
Q
Department of Plant Pathology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts, UK.
Chen
Z C
ZC
Antoniw
J F
JF
White
R F
RF
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D015342:DNA Probes / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009699:N-Glycosyl Hydrolases; D009841:Oligonucleotides / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D054789:Ribosome Inactivating Proteins, Type 1; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D009841:Oligonucleotides; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; C024275:pokeweed antiviral protein