title: [3H]acetazolamide binding to carbonic anhydrase in normal and transformed cells.
abstract: The binding of [3H]acetazolamide (AZ), a carbonic anhydrase (CA) inhibitor, to soluble and particulate forms of CA was investigated. Sources for the assays were purified CA II, adult rat cortical, oligodendrocyte and neuronal enriched preparations; cultured murine glial cells, rat C-6 glioma, rat hepatoma and human glioblastoma cells. CA enzyme activity in the same preparations was also assayed by following change in pH during incubation. A gel permeation chromatographic method was developed to assess [3H]AZ binding to soluble CA, while glass fiber filter vacuum filtration was used for particulate CA binding. Saturable specific binding of [3H]AZ to rat cortical soluble and particulate CA preparations was demonstrated. Computer-assisted data analysis estimated the binding parameters of [3H]AZ to soluble rat cortical CA to be Bmax = 0.38 +/- 0.13 pmol/mg protein and Kd = 34.7 +/- 17.5 nM. The rat cortical particulate fraction Bmax was 2.05 +/- 0.28 pmol/mg protein with a Kd of 107.1 +/- 24.2 nM. Purified bovine CA-II bound 1.15 +/- 0.19 pmol [3H]AZ/mg protein with a Kd of 54.0 +/- 3.4 nM. The pH optima for [3H]AZ binding to soluble and particulate CA was between 6.5 and 7.5. Binding was linear with respect to protein up to 1.0 mg/mL. The particulate fraction bound 3-4 times more [3H]ligand per unit protein than the soluble fraction. Interestingly, no detectable CA enzyme activity or [3H]AZ binding was observed in the soluble or particulate fractions of human glioblastoma, rat C-6 glioma or rat hepatoma cells. Binding of [3H]AZ to other soluble enzymes or proteins was negligible. In competition binding experiments, a rank order of inhibition of [3H]AZ binding to rat cortical CA by established CA inhibitors was: dichlorphenamide greater than acetazolamide greater than or equal to benzolamide greater than methazolamide greater than hydrochlorothiazide greater than or equal to sulfanilamide. [3H]AZ binding was not affected by other classes of pharmacologic characterizing agents. The binding of [3H]AZ to the CA enzyme molecule is highly specific and sensitive and may prove useful in vitro or in situ as a probe for this enzyme.
authors.affiliation: American Cyanamid Company, Medical Research Division, Lederle Laboratories, Pearl River, NY 10965.
authors.forename: L R
authors.initials: LR
authors.lastname: Meyerson
authors.forename: D
authors.initials: D
authors.lastname: Nesta
mesh_terms: D000086:Acetazolamide / Q000378:metabolism* / Q000493:pharmacokinetics; D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000201:enzymology*; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002417:Cattle; D002460:Cell Line / Q000201:enzymology; D002461:Cell Line, Transformed / Q000201:enzymology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D011506:Proteins / Q000378:metabolism / Q000494:pharmacology; D051381:Rats
chemical_list: D011506:Proteins; D002256:Carbonic Anhydrases; D000086:Acetazolamide