title: A dihydrofolate reductase gene from Candida albicans: molecular cloning.
abstract: The dihydrofolate reductase gene from Candida albicans has been cloned and partially characterized. A genomic bank from C. albicans strain 10127/5 was constructed in Escherichia coli and screened for trimethoprim resistance. A plasmid pMF1, carrying the resistance marker was isolated and characterized by restriction mapping and Southern blotting. Cells harbouring pMF1 were as sensitive as the parental cells to a wide spectrum of antibacterial agents, except for trimethoprim; the dihydrofolate reductase activity from these cells was trimethoprim resistant.
authors.affiliation: Department of Animal Biology and Genetics, University of Firenze, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Franceschi
authors.forename: M
authors.initials: M
authors.lastname: Denaro
authors.forename: T
authors.initials: T
authors.lastname: Irdani
authors.forename: R
authors.initials: R
authors.lastname: Lorenzetti
authors.forename: G
authors.initials: G
authors.lastname: Mastromei
authors.forename: E
authors.initials: E
authors.lastname: Skarmoutsou
authors.forename: M
authors.initials: M
authors.lastname: Polsinelli
mesh_terms: D002176:Candida albicans / Q000235:genetics*; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D010957:Plasmids; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D014295:Trimethoprim / Q000494:pharmacology
chemical_list: D014295:Trimethoprim; D013762:Tetrahydrofolate Dehydrogenase