Toxicity and activity of purified trichosanthin.
Trichosanthin was purified from fresh Chinese root tubers of Trichosanthes kirilowii and evaluated for anti-HIV activity. Trichosanthin inhibited syncytium formation between infected H9 cells and uninfected Sup-T1 cells from 0.5 to 4 micrograms/ml. Trichosanthin also inhibited HIV replication in H9 and CEM-SS cells at 1 microgram/ml, but was toxic for MT-4 cells (HTLV-I-positive), at doses greater than 0.25 microgram/ml. This new purification procedure confirms the anti-HIV activity of trichosanthin on some cell lines in different biological assays.
Ferrari
P
P
Laboratories Cassenne, Centre de Recherches Immunologiques Roussel-Uclaf, Cergy Pontoise, France.
Trabaud
M A
MA
Rommain
M
M
Mandine
E
E
Zalisz
R
R
Desgranges
C
C
Smets
P
P
D000596:Amino Acids / Q000032:analysis; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line / Q000187:drug effects; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D015726:Giant Cells / Q000166:cytology / Q000187:drug effects; D015497:HIV-1 / Q000502:physiology; D006801:Humans; D013601:T-Lymphocytes / Q000166:cytology; D013936:Thymidine / Q000378:metabolism; D015978:Trichosanthin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects
D000596:Amino Acids; D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D015978:Trichosanthin; D013936:Thymidine