title: Chlamydia pneumoniae elementary body antigenic reactivity with fluorescent antibody is destroyed by methanol.
abstract: Methanol, used for fixing chlamydia organisms to microscopic slides, was shown to eliminate the antigenic reactivity of Chlamydia pneumoniae elementary bodies with fluorescent antibodies. Acetone fixation had no adverse effect. Chlamydia trachomatis and Chlamydia psittaci were not affected by methanol fixation. The effect of methanol on C. pneumoniae is another characteristic that differentiates this species from the other two chlamydia species.
authors.affiliation: Department of Pathobiology, University of Washington, Seattle 98195.
authors.forename: S P
authors.initials: SP
authors.lastname: Wang
authors.forename: J T
authors.initials: JT
authors.lastname: Grayston
mesh_terms: D000942:Antigens, Bacterial; D002689:Chlamydia / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D005404:Fixatives; D005455:Fluorescent Antibody Technique; D006801:Humans; D000432:Methanol; D013045:Species Specificity
chemical_list: D000942:Antigens, Bacterial; D005404:Fixatives; D000432:Methanol