title: Trimerization of the reovirus cell attachment protein (sigma 1) induces conformational changes in sigma 1 necessary for its cell-binding function.
abstract: The implications of reovirus sigma l protein trimerization on its cell-binding function were investigated. Both monomeric and trimeric forms of sigma l were found to be present when full-length type 3 reovirus Sl transcripts prepared in vitro were translated in rabbit reticulocyte lysates. Pulse-chase experiments demonstrated that monomers were precursors of trimers. However, only the trimeric form was capable of binding to cell surface receptors. Protease and antibody recognition analyses revealed significant structural differences between these two sigma l forms at both the N- and C-termini. Our results suggest that trimerization of protein sigma l is accompanied by extensive conformational changes necessary for its cell attachment function.
authors.affiliation: Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, Alberta, Canada.
authors.forename: G
authors.initials: G
authors.lastname: Leone
authors.forename: R
authors.initials: R
authors.lastname: Duncan
authors.forename: P W
authors.initials: PW
authors.lastname: Lee
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000936:Antigen-Antibody Complex; D036022:Capsid Proteins; D066298:In Vitro Techniques; D007739:L Cells; D046911:Macromolecular Substances; D051379:Mice; D011487:Protein Conformation; D011991:Receptors, Virus / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D012087:Reoviridae / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014764:Viral Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D036022:Capsid Proteins; D046911:Macromolecular Substances; D011991:Receptors, Virus; D011994:Recombinant Proteins; D014764:Viral Proteins; C031728:sigma 1 protein, reovirus