Structure, function and flexibility of human lactoferrin.
X-ray structure analyses of four different forms of human lactoferrin (diferric, dicupric, an oxalate-substituted dicupric, and apo-lactoferrin), and of bovine diferric lactoferrin, have revealed various ways in which the protein structure adapts to different structural and functional states. Comparison of diferric and dicupric lactoferrins has shown that different metals can, through slight variations in the metal position, have different stereochemistries and anion coordination without any significant change in the protein structure. Substitution of oxalate for carbonate, as seen in the structure of a hybrid dicupric complex with oxalate in one site and carbonate in the other, shows that larger anions can be accommodated by small side-chain movements in the binding site. The multidomain nature of lactoferrin also allows rigid body movements. Comparison of human and bovine lactoferrins, and of these with rabbit serum transferrin, shows that the relative orientations of the two lobes in each molecule can vary; these variations may contribute to differences in their binding properties. The structure of apo-lactoferrin demonstrates the importance of large-scale domain movements for metal binding and release and suggests that in solution an equilibrium exists between open and closed forms, with the open form being the active binding species. These structural forms are shown to be similar to those seen for bacterial periplasmic binding proteins, and lead to a common model for the various steps in the binding process.
Baker
E N
EN
Department of Chemistry and Biochemistry, Massey University, Palmerston North, New Zealand.
Anderson
B F
BF
Baker
H M
HM
Haridas
M
M
Jameson
G B
GB
Norris
G E
GE
Rumball
S V
SV
Smith
C A
CA
D000818:Animals; D001059:Apoproteins / Q000737:chemistry; D001426:Bacterial Proteins / Q000378:metabolism; D002417:Cattle; D006801:Humans; D007501:Iron / Q000378:metabolism; D007781:Lactoferrin / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D015394:Molecular Structure; D011487:Protein Conformation; D013329:Structure-Activity Relationship; D014961:X-Ray Diffraction
D001059:Apoproteins; D001426:Bacterial Proteins; C039223:apolactoferrin; D007501:Iron; D007781:Lactoferrin