cDNA sequence and deduced amino acid sequence of human preprocolipase.
Complementary DNA clones for human pancreatic colipase were identified in human pancreatic cDNA libraries by hybridization with a pool of synthetic oligonucleotides containing all possible coding sequences for amino acids 75 to 80 of the partial human colipase protein sequence (Sternby, et al. Biochim Biophys Acta 1984;784:75). Alignment of overlapping cDNA clones yielded an mRNA sequence of 504 nucleotides [not including the poly(A) tail] encoding a polypeptide of 112 amino acids. The prepeptide comprised 17 amino acids, with an amino-terminal cluster of charged residues followed by a hydrophobic core of 12 residues typical of leader sequences. The deduced human procolipase sequence comprised 95 residues, including a propeptide of 5 residues. It was in complete agreement with the partial sequence previously obtained by protein sequencing. Northern blot analysis revealed that the polyadenylated preprocolipase transcript had a length of approximately 680 nucleotides.
Renaud
W
W
U315 INSERM de Physiologie et Pathologie Digestives, Marseille, France.
Dagorn
J C
JC
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003089:Colipases / Q000737:chemistry / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004792:Enzyme Precursors / Q000737:chemistry / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics
D003089:Colipases; D004792:Enzyme Precursors; D012333:RNA, Messenger; C070702:preprocolipase; D004247:DNA