Acetate catabolism in the dissimilatory iron-reducing isolate GS-15.
Acetate-grown GS-15 whole-cell suspensions were disrupted with detergent and assayed for enzymes associated with acetate catabolism. Carbon monoxide dehydrogenase and formate dehydrogenase were not observed in GS-15. Catabolic levels of acetokinase and phosphotransacetylase were observed. Enzyme activities of the citric acid cycle, i.e., isocitrate dehydrogenase, 2-oxoglutarate sythase, succinate dehydrogenase, fumarase, and malate dehydrogenase, were observed.
Champine
J E
JE
Department of Microbiology, University of Massachusetts, Amherst 01003.
Goodwin
S
S
D000084:Acetate Kinase / Q000032:analysis; D000085:Acetates / Q000378:metabolism*; D000105:Acetyl Coenzyme A / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D002248:Carbon Monoxide / Q000378:metabolism; D002952:Citric Acid Cycle; D004579:Electron Transport; D019605:Euryarchaeota / Q000201:enzymology / Q000378:metabolism; D005649:Fumarate Hydratase; D007521:Isocitrate Dehydrogenase / Q000032:analysis; D007658:Ketone Oxidoreductases / Q000032:analysis; D008291:Malate Dehydrogenase / Q000032:analysis; D010709:Phosphate Acetyltransferase / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000032:analysis
D000085:Acetates; D000105:Acetyl Coenzyme A; D002248:Carbon Monoxide; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D007658:Ketone Oxidoreductases; C023096:2-oxoglutarate synthase; D013385:Succinate Dehydrogenase; D010709:Phosphate Acetyltransferase; D000084:Acetate Kinase; D005649:Fumarate Hydratase