title: The proteolytic system of the fission yeast Schizosaccharomyces pombe.
abstract: Proteinase and peptidase activities of the fission yeast Schizosaccharomyces pombe were investigated. Several intracellular proteolytic enzymes were found: two endoproteinases, one carboxypeptidase, one aminopeptidase and one dipeptidyl-aminopeptidase. In addition, proteinase inhibitors were detected. In fresh crude extracts an activation procedure is needed to measure maximal activities of endoproteinases and carboxypeptidase, whose level is markedly dependent on growth medium composition and on growth phase, while aminopeptidase and dipeptidyl-aminopeptidase activities are very little, if at all, regulated by the carbon source.
authors.affiliation: Departamento de Biología Funcional, Universidad de Oviedo, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Suárez-Rendueles
authors.forename: L
authors.initials: L
authors.lastname: Villa
authors.forename: M J
authors.initials: MJ
authors.lastname: Arbesú
authors.forename: B
authors.initials: B
authors.lastname: Escudero
mesh_terms: D000626:Aminopeptidases / Q000378:metabolism; D002268:Carboxypeptidases / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism*; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D012568:Schizosaccharomyces / Q000201:enzymology*; D013696:Temperature
chemical_list: D002268:Carboxypeptidases; D010450:Endopeptidases; D000626:Aminopeptidases