title: Effect of dietary fish oil on development and selected functions of murine inflammatory macrophages.
abstract: Inflammatory macrophages from mice fed diets containing menhaden fish oil (MFO) have a reduced capacity for cytotoxicity of mastocytoma cells upon activation with interferon-gamma (IFN gamma) and lipopolysaccharide due to an altered responsiveness to IFN gamma. In an effort to elucidate further how dietary MFO effects macrophage function, we have studied the maturation of inflammatory macrophages from mice fed MFO compared with mice fed safflower oil (SFO) using several processes that serve as markers of the activational state. No significant differences in the recruitment or percentage of peritoneal exudate cells as macrophages after thioglycollate injection and no differences in spreading, binding, or phagocytosis of sheep erythrocytes or phagocytosis of yeast by inflammatory macrophages were observed when the dietary groups were compared. However, MFO macrophages had an altered capacity for peroxide release when stimulated with unopsonized zymosan (10-200 micrograms/ml). Furthermore, to elucidate how MFO feeding could alter IFN gamma-induced responses of inflammatory macrophages, we assessed phorbol-12-myristate-13-acetate-induced hydrogen peroxide production and expression of class II MHC determinants (Ia). There were no differences between macrophages from mice fed the two diets with respect to the production of peroxide when they were preincubated with 0.1-10 U/ml of IFN gamma. However, MFO macrophages had greater peroxide production after enhancement with 100 U/ml of IFN gamma. With respect to Ia induction, the percentage of macrophages responding to IFN gamma was not altered by diet, and there were no differences in expression of Ia induced by 24 hr exposure to IFN gamma. Thus the differential effect of MFO compared with SFO is probably mediated not by an alteration in the maturation of inflammatory macrophages but rather through the alteration of IFN gamma-induced functions such as peroxide production.
authors.affiliation: Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis 95616.
authors.forename: N E
authors.initials: NE
authors.lastname: Hubbard
authors.forename: S D
authors.initials: SD
authors.lastname: Somers
authors.forename: K L
authors.initials: KL
authors.lastname: Erickson
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage / Q000494:pharmacology*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007249:Inflammation / Q000473:pathology* / Q000503:physiopathology; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D051379:Mice; D010545:Peroxides / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology; D012450:Safflower Oil / Q000008:administration & dosage / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
chemical_list: D004042:Dietary Fats, Unsaturated; D005395:Fish Oils; D000949:Histocompatibility Antigens Class II; D008070:Lipopolysaccharides; D010545:Peroxides; D012450:Safflower Oil; D007371:Interferon-gamma; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate