title: Apolipoprotein A-I-containing lipoproteins in human umbilical cord blood. Relation to proapolipoprotein A-I and lecithin:cholesterol acyltransferase.
abstract: Lipids, apolipoproteins, lipoproteins, as well as lipoproteins containing both apo A-I and apo A-II (Lp A-I:A-II) or apo A-I but no apo A-II (Lp A-I), proapolipoprotein (proapo) A-I and the activity of lecithin:cholesterol acyltransferase (LCAT), were investigated in umbilical cord sera of 67 term human neonates (30 females and 37 males). Lp A-I and Lp A-I:A-II were present in umbilical cord sera with levels of 0.26 +/- 0.1 and 0.33 +/- 0.15 g/l, respectively. Furthermore, the absolute amount of proapo A-I was lower in cord blood than in adult plasma, but in view of the lower apo A-I levels in umbilical cord sera it comprised 10.48 +/- 3.86% of total apo A-I and was thus significantly higher than in adult plasma (7.1 +/- 0.9%). Proapo A-I was highly correlated with HDL cholesterol and apo A-I. Total serum LCAT activity was about 50% of adult plasma and was highly correlated with Lp A-I, but not with Lp A-I:A-II. We conclude that human umbilical cord serum contains both Lp A-I and Lp A-I:A-II particles and that the LCAT activity is predominantly related with the Lp A-I subfraction. The higher percentage in umbilical cord sera of proapo A-I may indicate a higher turnover of apo A-I or a lower activity of the proapo A-I cleaving enzyme which is still not identified.
authors.affiliation: SERLIA, INSERM U325, Institut Pasteur, Lille, France.
authors.forename: A
authors.initials: A
authors.lastname: Barkia
authors.forename: E
authors.initials: E
authors.lastname: Thiemann
authors.forename: P
authors.initials: P
authors.lastname: Czekelius
authors.forename: A
authors.initials: A
authors.lastname: Wiegel
authors.forename: A
authors.initials: A
authors.lastname: Leroy
authors.forename: J C
authors.initials: JC
authors.lastname: Fruchart
authors.forename: A
authors.initials: A
authors.lastname: Steinmetz
mesh_terms: D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000097:blood*; D008076:Cholesterol, HDL / Q000097:blood; D005260:Female; D005312:Fetal Blood / Q000378:metabolism*; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood; D011498:Protein Precursors / Q000097:blood
chemical_list: D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D008076:Cholesterol, HDL; D008074:Lipoproteins; D011498:Protein Precursors; D007862:Phosphatidylcholine-Sterol O-Acyltransferase