title: The effect of alpha-tocopherol as an antioxidant on the oxidation of membrane protein thiols induced by free radicals generated in different sites.
abstract: Azo compounds enable us to generate peroxyl radicals by thermal decomposition at a constant rate and at a desired site, that is, water-soluble compounds produce initiating radicals in an aqueous phase and lipid-soluble compounds initiate the oxidation within the membrane-lipid layer. Using these radicals generated in different sites, we oxidized red blood cell ghost membranes to study the relationships between alpha-tocopherol depletion, initiation of lipid peroxidation, and protein damage. When radicals were generated in the aqueous phase, the loss of membrane protein thiols was observed concurrently with the consumption of membrane tocopherol and after tocopherol was exhausted the peroxidation of membrane lipids occurred. On the other hand, when radicals were initiated within the lipid region, the oxidation of thiols and the formation of thiobarbituric acid-reactive substances were suppressed to give an induction period until tocopherol fell below a critical level. Our results indicate that the surface thiols of extrinsic proteins may compete with alpha-tocopherol for trapping aqueous radicals and spare tocopherol to some extent, whereas the oxidation of intrinsic buried thiols may commence due to lipid-derived radicals produced after tocopherol was consumed. In conclusion, alpha-tocopherol in the membrane can break the free radical chain efficiently to inhibit the lipid peroxidation. However, the effect of tocopherol on the inhibition of membrane protein damage, exhibited by the loss of thiols and the formation of high-molecular-weight proteins, would be different depending on the site of initial radical generation.
authors.affiliation: Department of Pediatrics, Osaka Medical College, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Takenaka
authors.forename: M
authors.initials: M
authors.lastname: Miki
authors.forename: H
authors.initials: H
authors.lastname: Yasuda
authors.forename: M
authors.initials: M
authors.lastname: Mino
mesh_terms: D000578:Amidines / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001391:Azo Compounds / Q000494:pharmacology; D001798:Blood Proteins / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D005609:Free Radicals; D007700:Kinetics; D015227:Lipid Peroxidation; D008297:Male; D009570:Nitriles / Q000494:pharmacology; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D013438:Sulfhydryl Compounds / Q000097:blood*; D013858:Thiobarbiturates; D014810:Vitamin E / Q000097:blood / Q000494:pharmacology*
chemical_list: D000578:Amidines; D000975:Antioxidants; D001391:Azo Compounds; D001798:Blood Proteins; D005609:Free Radicals; D009570:Nitriles; D013438:Sulfhydryl Compounds; D013858:Thiobarbiturates; C055792:2,2'-azobis(2,4-dimethylvaleronitrile); D014810:Vitamin E; C046728:2,2'-azobis(2-amidinopropane); C029684:thiobarbituric acid