Identity of mucin's "118-kDa link protein" with fibronectin fragment.
Human and rat intestinal mucin was purified by equilibrium density gradient centrifugation and Sepharose 2B chromatography according to M. Mantle, D. Mantle, and A. Allen (1981, Biochem. J. 195, 277-285) and analyzed using mucin, DNA, and fibronectin-specific antibodies in dot-blot, ELISA, and Western blotting. The 118-kDa component of the mucins and the 118-kDa fragment of fibronectin from the same source displayed affinity for concanavalin A and immunoreacted with fibronectin antibodies. The amino acid and carbohydrate compositions of the 118-kDa peptide electroeluted by gel electrophoresis of mucin and fibronectin preparations were identical within each pair of glycopeptides and closely resembled the "link protein component" of human and rat intestinal mucin preparations of R. E. F. Fahim, R. D. Specian, G. G. Forstner, and J. F. Forstner (1987, Biochem. J. 243, 631-640) and M. Mantle and G. Stewart (1989, Biochem. J. 259, 631-640). We therefore conclude that the "link protein" claimed to be an integral part of mucus glycoproteins in actuality is the 118-kDa fragment of fibronectin.
Slomiany
A
A
Research Center, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
Okazaki
K
K
Tamura
S
S
Slomiany
B L
BL
D000596:Amino Acids / Q000032:analysis; D000906:Antibodies; D002241:Carbohydrates / Q000032:analysis; D003550:Cystic Fibrosis / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000737:chemistry*; D006020:Glycopeptides / Q000302:isolation & purification; D006801:Humans; D007413:Intestinal Mucosa / Q000737:chemistry*; D007421:Intestine, Small; D008970:Molecular Weight; D009077:Mucins / Q000737:chemistry* / Q000302:isolation & purification
D000596:Amino Acids; D000906:Antibodies; D002241:Carbohydrates; D005353:Fibronectins; D006020:Glycopeptides; D009077:Mucins