title: Methanogenic pathways in Methanosphaera stadtmanae.
abstract: Methanosphaera stadtmanae reduces methanol to CH4 in a similar way as Methanosarcina barkeri. Low activities of 5,10-methylenetetrahydromethanopterin dehydrogenase (MTDH) and reductase (MTR) were found. From studies on formaldehyde oxidation and reduction it was concluded that most likely the inability to reduce CO2 to CH4 was due to the lack of an active or the presence of an inactive CO2 reductase system and methyltetrahydromethanopterin (methyl-H4MPT): coenzyme M methyltransferase. Methanofuran was not detected, while the presence of a pterin, analogous to H4MPT, could be substantiated from its degradation products in boiled extracts.
authors.affiliation: Department of Microbiology, Faculty of Science, University of Nijmegen, The Netherlands.
authors.forename: W M
authors.initials: WM
authors.lastname: van de Wijngaard
authors.forename: J
authors.initials: J
authors.lastname: Creemers
authors.forename: G D
authors.initials: GD
authors.lastname: Vogels
authors.forename: C
authors.initials: C
authors.lastname: van der Drift
mesh_terms: D002245:Carbon Dioxide / Q000378:metabolism; D019605:Euryarchaeota / Q000378:metabolism*; D008697:Methane / Q000378:metabolism; D000432:Methanol / Q000378:metabolism*
chemical_list: D002245:Carbon Dioxide; D008697:Methane; D000432:Methanol