[Site-directed mutagenesis in the uracil-repair system. Preparation of mutated forms of human alpha 2 interferon].
The mutant forms of human IFN-alpha 2 gene are obtained by oligonucleotide-directed mutagenesis with the use of uracil-repair system. To intensify the process the procedure of the uracil-containing DNA template preparation is modified. It was determined that when mutagenesis is performed in the uracil-repair system the yield of the process depends on the mutant DNA-strand in vitro synthesis efficiency. It is shown that the stability of the 5'-end primer-template complex and the level of the endogenic primers elongation are the basis factors, that determine induction mutations.
Shekhter
I I
II
Veĭko
V P
VP
Bulenkov
M T
MT
Ratmanova
K I
KI
Debabov
V G
VG
D000818:Animals; D001483:Base Sequence; D003090:Coliphages / Q000235:genetics; D004247:DNA / Q000235:genetics; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015967:Gene Expression Regulation, Viral; D005798:Genes, Bacterial; D005814:Genes, Viral; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011994:Recombinant Proteins; D013552:Swine; D013698:Templates, Genetic; D014498:Uracil / Q000378:metabolism*
D007370:Interferon Type I; D011994:Recombinant Proteins; D014498:Uracil; D004247:DNA
Saĭt-napravlennyĭ mutagenez v uratsil-reparatsionnoĭ sisteme. Poluchenie mutantnykh form interferon alpha2 cheloveka.