Detection of neutral sugars in purified type G botulinum progenitor toxin and the effects of some glycolytic enzymes on its molecular dissociation and oral toxicity.
Arabinose and galactose were detected in purified type G botulinum toxin (Mr about 500,000) of Clostridium argentinense. The i.p. LD50/mg N of type G progenitor toxin was one-tenth, but the oral LD50/mg N twice that of type A-L toxin. The lysozyme-, endo-beta-galactosidase-, and N-glucanase-treated toxins each had a molecular mass of about 300,000. The oral toxicity of the endo-beta-galactosidase or N-glucanase-treated toxin was one-fifth that of untreated progenitor toxin. On DEAE-Sephadex chromatography, the N-glucanase-treated toxin dissociated into two fractions, nontoxic and toxic. SDS-PAGE of the toxic fraction showed a single band with a Mr of about 150,000, and after dithiothreitol treatment, two bands with Mr of 100,000 and 50,000.
Nukina
M
M
Public Health Institute of Kobe City, Hyogo, Japan.
Miyata
T
T
Sakaguchi
S
S
Sakaguchi
G
G
D000284:Administration, Oral; D000818:Animals; D001089:Arabinose / Q000378:metabolism*; D001905:Botulinum Toxins / Q000737:chemistry* / Q000633:toxicity; D002473:Cell Wall / Q000378:metabolism; D002849:Chromatography, Gas; D003013:Clostridium / Q000032:analysis* / Q000472:pathogenicity; D004229:Dithiothreitol / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005690:Galactose / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D051379:Mice; D009113:Muramidase / Q000378:metabolism; D010457:Peptidoglycan / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D010457:Peptidoglycan; D001089:Arabinose; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D009113:Muramidase; D001616:beta-Galactosidase; D001905:Botulinum Toxins; D004229:Dithiothreitol; D005690:Galactose