title: High-performance liquid chromatographic determination of glyphosate and (aminomethyl)phosphonic acid in human serum after conversion into p-toluenesulphonyl derivatives.
abstract: We have developed a simple, highly sensitive and fast assay method for determining glyphosate and its major metabolite, (aminomethyl)phosphonic acid (AMPA), in serum by high-performance liquid chromatography with ultraviolet detection. Both compounds were successfully extracted with an anion-exchange resin column and allowed to react with p-toluenesulphonyl chloride. The detection limits were 0.3 microgram/ml for glyphosate and 0.2 microgram/ml for AMPA. Recoveries of glyphosate and AMPA spiked to serum were ca. 75% and ca. 88%, respectively. We are convinced that this procedure, in practice, allows medical examiners to analyse both compounds in the serum of poisoned patients within a short time.
authors.affiliation: Department of Legal Medicine, Kawasaki Medical School, Kurashiki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tomita
authors.forename: T
authors.initials: T
authors.lastname: Okuyama
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: B
authors.initials: B
authors.lastname: Uno
authors.forename: S
authors.initials: S
authors.lastname: Kawai
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005998:Glycine / Q000031:analogs & derivatives* / Q000097:blood; D006540:Herbicides / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008832:Microchemistry; D009943:Organophosphorus Compounds / Q000097:blood*; D014105:Tosyl Compounds; D000097797:Glyphosate
chemical_list: D005998:Glycine; D006540:Herbicides; D009943:Organophosphorus Compounds; D014105:Tosyl Compounds; D000097797:Glyphosate; C025506:4-toluenesulfonyl chloride; C036423:1-aminomethylphosphonic acid