Formaldehyde-resistance in Enterobacteriaceae and Pseudomonas aeruginosa: identification of resistance genes by DNA-hybridization.
A 4.1. Kb large DNA fragment of a E. coli plasmid pVU 3695, on which the genes for formaldehyde-resistance are located, was used as a DNA probe to identify bacteria that carry this segment among formaldehyde-resistant bacteria. It was shown by Southern Blot-, Dot Blot-, and Colony Blot- Hybridization studies that the DNA of all formaldehyde-resistant E. coli, Serratia marcescens, Enterobacter cloacae, Citrobacter freundii and Klebsiella pneumoniae strains tested hybridize with the DNA probe from E. coli. In contrast the E. coli DNA probe does not hybridize with the DNA from formaldehyde-resistant Pseudomonas aeruginosa strains.
Wollmann
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A
Institut für Med. Mikrobiologie und Immunologie der Universität Hamburg.
Kaulfers
P M
PM
D015139:Blotting, Southern; D002954:Citrobacter / Q000187:drug effects / Q000235:genetics; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D004352:Drug Resistance, Microbial / Q000235:genetics; D004754:Enterobacter / Q000187:drug effects / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005557:Formaldehyde / Q000494:pharmacology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics; D009693:Nucleic Acid Hybridization; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics; D011815:R Factors; D015183:Restriction Mapping; D012706:Serratia marcescens / Q000187:drug effects / Q000235:genetics
D015342:DNA Probes; D004269:DNA, Bacterial; D005557:Formaldehyde