Relationship of the uptake and beta-oxidation of 18-carbon fatty acids with stimulation of murine mammary tumor cell growth.
The effects of stearic (18:0), oleic (18:1) and linoleic (18:2) acid on the in vitro growth of murine mammary tumor cell line 4526 were compared to 4526 uptake and beta-oxidation of those fatty acids. Both 18:1 and 18:2 stimulated, while 18:0 inhibited growth. Likewise, uptake and oxidation rates were much lower for 18:0 than for 18:1 and 18:2. The influence of glucose, insulin and fatty acid-depletion on 4526 fatty acid incorporation and oxidation was also determined. The correlation between effects on in vitro growth and ability to assimilate 18:0, 18:1 and 18:2 may partially explain the stimulation by unsaturated and inhibition by saturated fatty acids of in vivo 4526 tumorigenesis.
Buckman
D K
DK
Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis 95616-8643.
Erickson
K L
KL
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002455:Cell Division; D005227:Fatty Acids / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010084:Oxidation-Reduction; D014407:Tumor Cells, Cultured
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D007328:Insulin; D005947:Glucose