Cure with low-dose total-body irradiation of the hematological disorder induced in mice with the Friend virus: possible mechanism involving interferon-gamma and interleukin-2.
The effects of split low-dose total-body irradiation (TBI; 150 cGy) on production of interferon (IFN)-gamma and Interleukin-2 (IL-2) and on the growth characteristics of erythroid progenitor cells (BFU-E) have been assessed in normal mice, normal mice receiving TBI only, mice infected with the polycythemia-inducing strain of the Friend virus complex (FVC-P), and FVC-P infected mice receiving 150 cGy TBI on days 5 and 12. It was found that lymphocytes from the spleens of TBI-treated mice previously infected with FVC-P produced in response to phytohemagglutinin (PHA) and concanavalin A (Con A) stimulation up to 15 times greater amounts of IFN-gamma than cells from untreated FVC-P-infected mice. IL-2 production in Con A-stimulated spleen cell cultures also increased when cells were isolated from FVC-P-infected mice treated by low-dose TBI compared to untreated FVC-P-infected mice. TBI treatment was associated with greater than 99% ablation of "erythropoietin-independent" BFU-E colony formation. The results suggest that the cure of FVC-P-infected mice by low-dose TBI may result from activation of the IFN-gamma system and IL-2 production.
Shen
R N
RN
Department of Medicine (Hematology/Oncology), Indiana University School of Medicine, Indianapolis 46202.
Lu
L
L
Feng
G S
GS
Miller
J
J
Taylor
M W
MW
Broxmeyer
H E
HE
D000818:Animals; D003114:Colony-Forming Units Assay; D004306:Dose-Response Relationship, Immunologic; D015672:Erythroid Precursor Cells / Q000166:cytology; D004921:Erythropoietin / Q000502:physiology; D005260:Female; D005622:Friend murine leukemia virus; D007371:Interferon-gamma / Q000032:analysis / Q000502:physiology*; D007376:Interleukin-2 / Q000032:analysis / Q000502:physiology*; D007942:Leukemia, Experimental / Q000276:immunology / Q000382:microbiology / Q000532:radiotherapy*; D051379:Mice; D008811:Mice, Inbred DBA; D014916:Whole-Body Irradiation / Q000379:methods*
D007376:Interleukin-2; D004921:Erythropoietin; D007371:Interferon-gamma