Hepatic transport of the magnetic resonance imaging contrast agent Fe(III)-N-(3-phenylglutaryl)desferrioxamine B.
We have studied the hepatic transport of Fe(III)-N-(3-phenylglutaryl)desferrioxamine B (Fe-PGDF). Using 59Fe-PGDF in biliary cannulated rats we have shown that 32 +/- 2% of an iv bolus dose was excreted into the bile. In animals pretreated with a saturating dose of taurocholate, oxyphenonium, or bromosulfophthalein (BSP), 25 +/- 6%, 23 +/- 1%, or 1.6 +/- 0.8% of the dose, respectively, was excreted into the bile. Magnetic resonance images indicated that BSP blocked uptake of Fe-PGDF by the hepatocytes. Possible enterohepatic recycling of 59Fe-PGDF was investigated in linked rat experiments. All of the material excreted into the bile of the donor, 28 +/- 10% of the dose, was recovered in the gastrointestinal tract of the recipient, but none was found in its bile or urine. These results suggested that uptake of Fe-PGDF by the hepatocytes occurred via the BSP transporter and that no enterohepatic recycling of the contrast agent occurred.
Hoener
B A
BA
Department of Pharmacy, School of Pharmacy, University of California, San Francisco 94143.
Tzika
A A
AA
Englestad
B L
BL
White
D L
DL
D000818:Animals; D001646:Bile / Q000737:chemistry / Q000378:metabolism; D001652:Bile Ducts / Q000378:metabolism; D003287:Contrast Media; D003676:Deferoxamine / Q000378:metabolism / Q000031:analogs & derivatives; D004386:Duodenum / Q000473:pathology; D007089:Image Enhancement; D007422:Intestines / Q000473:pathology; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008279:Magnetic Resonance Imaging; D008297:Male; D009942:Organometallic Compounds / Q000378:metabolism*; D010115:Oxyphenonium / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000473:pathology; D013448:Sulfobromophthalein / Q000378:metabolism; D013656:Taurocholic Acid / Q000378:metabolism; D001743:Urinary Bladder / Q000473:pathology
D003287:Contrast Media; D009942:Organometallic Compounds; D013448:Sulfobromophthalein; C069592:ferric-N-(3-phenylglutaryl)desferrioxamine B; D013656:Taurocholic Acid; D010115:Oxyphenonium; D003676:Deferoxamine