title: Variable inactivation of human factor VIII from different sources by human factor VIII inhibitors.
abstract: The source of human factor VIIII (FVIII) had a marked effect on the inhibitory activity of a panel of eight human FVIII inhibitors. Use of conventional FVIII concentrates gave lower inhibitor titres whereas a monoclonal antibody purified FVIII concentrate gave titres similar to or greater than those with plasma. Addition of phospholipid (PL) protected highly purified FVIII against inhibition. The content of PL-bound FVIII in concentrates may account for the observed differences.
authors.affiliation: Department of Clinical Veterinary Medicine, University of Cambridge.
authors.forename: J D
authors.initials: JD
authors.lastname: Littlewood
authors.forename: S A
authors.initials: SA
authors.lastname: Bevan
authors.forename: G
authors.initials: G
authors.lastname: Kemball-Cook
authors.forename: R J
authors.initials: RJ
authors.lastname: Evans
authors.forename: T W
authors.initials: TW
authors.lastname: Barrowcliffe
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000096:biosynthesis / Q000502:physiology*; D004285:Dogs; D005169:Factor VIII / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D010743:Phospholipids / Q000378:metabolism / Q000494:pharmacology; D015203:Reproducibility of Results; D014841:von Willebrand Factor / Q000502:physiology
chemical_list: D001323:Autoantibodies; D010743:Phospholipids; D014841:von Willebrand Factor; D005169:Factor VIII