The Xenopus YB3 protein binds the B box element of the class III promoter.
We have isolated a Xenopus cDNA encoding the YB3 protein which binds specifically to the B box promoter element of class III genes. Northern analysis shows YB3 is expressed in a variety of adult tissues. Fractionation of oocyte S150 extracts demonstrates YB3 is present in phosphocellulose fraction IIIC, as well as in the fraction isolated by B box DNA affinity chromatography. Silver staining indicates that YB3, or a protein of the same mobility in SDS gels, is the most abundant component in either fraction.
Cohen
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La Jolla Cancer Research Foundation, CA 92037.
Reynolds
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D000256:Adenoviridae / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002458:Cell Fractionation; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D009865:Oocytes / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics / Q000502:physiology*; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors; D014981:Xenopus / Q000235:genetics*; D029867:Xenopus Proteins
D004268:DNA-Binding Proteins; D014157:Transcription Factors; D029867:Xenopus Proteins; C070854:YB3 protein, Xenopus