title: Aminoacetone synthase from goat liver. Involvement of arginine residue at the active site and on the stability of the enzyme.
abstract: The arginine-specific reagents phenylglyoxal and butane-2,3-dione inactivated goat liver aminoacetone synthase with pseudo-first-order kinetics, with the rate dependent on modifier concentration. Phenylglyoxal and butane-2,3-dione appeared to react with one arginine residue per enzyme molecule. The inactivated enzyme could be re-activated by Tris, suggesting additional evidence of modification of the arginine residue. Acetyl-CoA, one of the substrates, completely protected the enzyme from inactivation. Glycine gave partial protection. Protection by substrates against inactivation by phenylglyoxal and butane-2,3-dione suggested the presence of an essential arginine residue at the substrate-binding region. Experiments with [7-14C]phenylglyoxal in the presence of acetyl-CoA showed that only the arginine residue at the active site could be modified by phenylglyoxal. The stability of the enzyme is dependent on the presence of both EDTA and Mg2+.
authors.affiliation: Department of Biochemistry, University College of Science, University of Calcutta, India.
authors.forename: S
authors.initials: S
authors.lastname: Ray
authors.forename: D
authors.initials: D
authors.lastname: Sarkar
authors.forename: M
authors.initials: M
authors.lastname: Ray
mesh_terms: D000105:Acetyl Coenzyme A / Q000494:pharmacology; D000123:Acetyltransferases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001120:Arginine; D001665:Binding Sites; D003931:Diacetyl / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D004793:Enzyme Reactivators; D004795:Enzyme Stability; D005998:Glycine / Q000494:pharmacology; D006041:Goats / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D010658:Phenylglyoxal / Q000494:pharmacology
chemical_list: D004793:Enzyme Reactivators; D000105:Acetyl Coenzyme A; D001120:Arginine; D004492:Edetic Acid; D000123:Acetyltransferases; C020679:glycine acetyltransferase; D008274:Magnesium; D003931:Diacetyl; D010658:Phenylglyoxal; D005998:Glycine