Radioimmunoassay of parathyroid hormone in cats.
A radioimmunoassay for measurement of midmolecule parathyroid hormone (PTH) concentration in serum from dogs was validated for use on serum from cats. The assay detected an increase in serum concentration of PTH after IV infusion of Na2 EDTA in healthy cats. Infusion of calcium chloride caused a decrease in measured PTH. Accuracy of the assay was demonstrated by quantitative recovery of a feline parathyroid gland extract added to pooled feline sera. Mean interassay and intra-assay coefficients of variation were 0.13 and 0.07, respectively. Sensitivity of the assay was 0.1 ng of PTH/ml. The median PTH concentration measured in 40 adult cats was 3.5 ng/ml, with a range of 1.16 to 11.0 ng/ml.
Flanders
J A
JA
Department of Clinical Sciences, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Reimers
T J
TJ
D000818:Animals; D002118:Calcium / Q000097:blood; D002122:Calcium Chloride / Q000008:administration & dosage / Q000494:pharmacology; D002415:Cats / Q000097:blood*; D004492:Edetic Acid / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D007262:Infusions, Intravenous / Q000662:veterinary; D008297:Male; D010281:Parathyroid Hormone / Q000097:blood*; D011237:Predictive Value of Tests; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D012016:Reference Values; D015203:Reproducibility of Results
D010281:Parathyroid Hormone; D011933:Reagent Kits, Diagnostic; D004492:Edetic Acid; D002122:Calcium Chloride; D002118:Calcium