title: A device for freeze-substituting a large number of samples under controlled conditions.
abstract: An inexpensive device for reproducible freeze-substitution is described. The equipment consists of a reciprocating shaker, holding vials of substitution fluid, mounted in the gas phase of a large liquid nitrogen refrigerator. The shaker temperature is controlled by a programmable microprocessor.
authors.affiliation: Department of Zoology, La Trobe University, Bundoora, Victoria, Australia.
authors.forename: A T
authors.initials: AT
authors.lastname: Marshall
authors.forename: M
authors.initials: M
authors.lastname: Kent
mesh_terms: D000818:Animals; D005615:Freezing; D008099:Liver / Q000648:ultrastructure*; D009584:Nitrogen; D051381:Rats; D015203:Reproducibility of Results; D013048:Specimen Handling
chemical_list: D009584:Nitrogen