Molecular cloning of cDNA for antimycin A-inducible mRNA and its role in cyanide-resistant respiration in Hansenula anomala.
A cDNA for mRNA induced by antimycin A in Hansenula anomala was cloned. The mRNA for the cDNA was expressed in the yeast under the conditions expressing the cyanide-resistant respiration activity. The nucleotide sequence revealed a long open reading frame of 342 codons encoding a protein with a molecular weight of 40,282 in the cDNA. An antibody recognizing the protein encoded by the open reading frame was produced. Immunoblotting of H. anomala proteins with this antibody showed that a 36 kDa protein localized in mitochondria was a mature form of the protein encoded by the cDNA. It is suggested that the cloned cDNA encodes a protein involved in the cyanide-resistant respiratory pathway.
Sakajo
S
S
Department of Biochemistry, Niigata College of Pharmacy, Japan.
Minagawa
N
N
Komiyama
T
T
Yoshimoto
A
A
D000595:Amino Acid Sequence; D000968:Antimycin A / Q000494:pharmacology*; D001483:Base Sequence; D003001:Cloning, Molecular; D003486:Cyanides / Q000494:pharmacology; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D015151:Immunoblotting; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics; D010101:Oxygen Consumption; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D015183:Restriction Mapping
D003486:Cyanides; D005656:Fungal Proteins; D012333:RNA, Messenger; D000968:Antimycin A; D010088:Oxidoreductases