[Immunoturbidimetric determination of C-3 components of complement systems using nonlinear calibration].
A method for the immunoturbidimetric analysis of the C-3-component in the complement system was elaborated by means of the antiserum Q-SwAHu/C3 USOL (SEVAG) Praha. A diluted human control serum USOL (SEVAG) Praha with declared values of plasma proteins was applied as a standard solution. The relation between concentration and absorption in an eight step calibration series is well described by a parabola of the 2nd degree. The precision in series and the accuracy of the method are mentioned. The proposed technique is in a relatively good correlation with the radial immunodiffusion according to MANCINI.
Rohácek
J
J
Abteilung der Klinischen Biochemie, CSFR.
Semrád
V
V
Klierová
E
E
Zápotocná
M
M
D001798:Blood Proteins / Q000032:analysis; D003176:Complement C3 / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D005779:Immunodiffusion / Q000379:methods; D007223:Infant; D009391:Nephelometry and Turbidimetry / Q000379:methods; D012015:Reference Standards
D001798:Blood Proteins; D003176:Complement C3
Immunoturbidimetrische Bestimmung der C-3-Komponente des Komplementsystems bei nichtlinearer Eichung.