Identification of a telomeric fragment from the right arm of chromosome III of Aspergillus nidulans.
A minimum of 11 bands hybridising to an oligonucleotide complementary to the putative telomeric repeat sequence (TTAGGG)n was visible in a Southern blot of EcoRI-digested Aspergillus nidulans genomic DNA. All 11 were sensitive to BAL 31 exonuclease digestion, consistent with telomeric locations. Blots of DNA from aneuploid strains deleted for a dispensable, extreme distal region on the right arm of chromosome III lack a 1.3-kb EcoRI band, indicating that this fragment is located at or near the chromosome III right arm telomere.
Connelly
J C
JC
Department of Bacteriology, Royal Postgraduate Medical School, London, U.K.
Arst
H N
HN
D001230:Aspergillus / Q000235:genetics*; D001483:Base Sequence; D015825:Chromosomes, Fungal; D004269:DNA, Bacterial / Q000032:analysis*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid
D004269:DNA, Bacterial