title: Application of an ovomucoid-conjugated polymer column for the enantiospecific determination of chlorprenaline concentrations in plasma.
abstract: An ovomucoid-conjugated polymer column was prepared for the liquid chromatographic resolution of racemic compounds. The column showed strong retention of acidic solutes, a characteristic attributed to the structure of the stationary phase support gel. Although the efficiency of the column was lower than that of an ovomucoid-conjugated silica gel column, enantiospecific chlorprenaline determination in plasma was achieved with solute amounts from 1.0 ng to 0.1 microgram.
authors.affiliation: Eisai Co., Ltd., Pharmaceutical Research Laboratory, Gifu, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Miwa
authors.forename: S
authors.initials: S
authors.lastname: Sakashita
authors.forename: H
authors.initials: H
authors.lastname: Ozawa
authors.forename: J
authors.initials: J
authors.lastname: Haginaka
authors.forename: N
authors.initials: N
authors.lastname: Asakawa
authors.forename: Y
authors.initials: Y
authors.lastname: Miyake
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D000431:Ethanol; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007545:Isoproterenol / Q000031:analogs & derivatives* / Q000097:blood; D008832:Microchemistry; D010059:Ovomucin; D011108:Polymers; D013237:Stereoisomerism
chemical_list: D011108:Polymers; D010059:Ovomucin; D000431:Ethanol; C004350:clorprenaline; D007545:Isoproterenol