Amino acid sequence determination of the novel forms of Go alpha purified from bovine brain membranes.
Previously we have reported that there are at least four different forms of Go alpha in bovine brain membranes which can be distinguished by their elution profiles from Mono Q column and their immunological reactivities. The four alpha-subunits are referred to as alpha o1, alpha o2, alpha o3 and alpha o4 in their elution orders from the column. Partial amino acid sequences of the purified alpha o1 and alpha o2 were determined and compared with the predicted sequences of two classes of Go alpha cDNAs, termed Go alpha-1 and Go alpha-2. There were at least two unique fragments corresponding with the predicted amino acid sequence of the Go alpha-2 cDNA but different from that of the Go alpha-1 cDNA upon tryptic digestion of alpha o1- or alpha o2-subunit. The alpha o3- and alpha o4-subunits, but not alpha o1-and alpha o2-subunits, were recognized by an antibody raised against a unique amino acid sequence predicted from Go alpha-1 cDNA. These results suggest that alpha o1,2 subunits and alpha o3,4 subunits are encoded by Go alpha-2 cDNA and Go alpha-1 cDNA, respectively.
Shibasaki
H
H
Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.
Kozasa
T
T
Takahashi
K
K
Inanobe
A
A
Kaziro
Y
Y
Ui
M
M
Katada
T
T
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D001923:Brain Chemistry; D002417:Cattle; D002462:Cell Membrane / Q000737:chemistry; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D014357:Trypsin / Q000378:metabolism
D046911:Macromolecular Substances; D014357:Trypsin; D019204:GTP-Binding Proteins