Nucleoside uptake in macrophages from various murine strains: a short-time and a two-step stimulation model.
Kinetics of [3H]-uridine uptake by murine peritoneal macrophages (pM phi) is early altered after exposure to a variety of stimuli. Alterations caused by Candida albicans, lipopolysaccharide (LPS) and recombinant interferon-gamma (rIFN-gamma) were similar in SAVO, C57BL/6, C3H/HeN and C3H/HeJ mice, and were not correlated with an activation process as shown by the amount of tumor necrosis factor-alpha (TNF-alpha) being released. Short-time exposure to all stimuli resulted in an increased nucleoside uptake by SAVO pM phi, suggesting that the tumoricidal function of this cell either depends from the type of stimulus or the time when the specific interaction with the cell receptor is taking place. Experiments with priming and triggering signals confirmed the above findings, indicating that the increase or the decrease of nucleoside uptake into the cell depends essentially on the chemical nature of the priming stimulus. The triggering stimulus, on the other hand, is only able to amplify the primary response.
Busolo
F
F
Institute of Microbiology of Padua University, Faculty of Medicine, Italy.
Conventi
L
L
Grigolon
M
M
Palù
G
G
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002176:Candida albicans; D002250:Carbon Radioisotopes; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D011865:Radioisotope Dilution Technique; D011994:Recombinant Proteins; D013045:Species Specificity; D014316:Tritium; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014529:Uridine / Q000378:metabolism*
D002250:Carbon Radioisotopes; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D014316:Tritium; D007371:Interferon-gamma; D007930:Leucine; D014529:Uridine