Heterogeneity of the breakpoint localization in malignant cells with a 9p11 chromosomal abnormality.
The p11 band of the short arm of chromosome 9 is involved in various cytogenetic alterations occurring in several malignant diseases. Using probes isolated from the 9p11 band in the study of a case of alpha-heavy-chain disease (MAL) with t(9;14)(p11;q32), we studied the DNA from seven malignant cell samples, including four cases of acute lymphoblastic leukemia with tdic(9;12)(p11;p12). Using pulsed-field electrophoresis analysis we demonstrated that the breakpoints were 3-300 kb distant from the original MAL breakpoint without clustering within the subset of leukemias with the tdic(9;12).
Pellet
P
P
Laboratory of Immunochemistry and Immunology (INSERM U 108), Hôpital Saint-Louis, Paris, France.
Hillion
J
J
Carroll
A J
AJ
Crist
W M
WM
Brouet
J C
JC
Tsapis
A
A
D002869:Chromosome Aberrations; D002873:Chromosome Fragility; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D002883:Chromosomes, Human, Pair 14; D002899:Chromosomes, Human, Pair 9; D004586:Electrophoresis / Q000379:methods; D006362:Heavy Chain Disease / Q000235:genetics; D006801:Humans; D007137:Immunoglobulin alpha-Chains; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D014178:Translocation, Genetic
D007137:Immunoglobulin alpha-Chains