In vitro effects of polychlorinated biphenyls on human platelets.
Incubation of human platelets with polychlorinated biphenyls (PCB) induced and modulated cellular responses to a different degree. 3,3',4,4'-tetrachlorobiphenyl (TCB) was a more potent inducer of platelet aggregation, serotonin release and 12-HETE generation compared to the other PCB [2,2',3,3'-TCB,3,3'-dichlorobiphenyl (DCB),2,2',4,5,5'-pentachlorobiphenyl (PCB)]. 3,3',4,4'-TCB showed synergistic effects, in combination with other PCB, such as an enhanced formation of 12-HETE, when 3,3'-DCB and 2,2',3,3'-TCB were applied simultaneously. The combined incubation of platelets with PCB and sodium fluoride (NaF), an activator of G-proteins, resulted in synergistic 12-HETE generation compared to stimulation with NaF or PCB alone. Furthermore, when platelets were incubated with the PCB the enzymatic steps controlling the metabolism of the platelet-activating factor (PAF) were modulated. A direct relationship between the extent of platelet activation and the chloro-substitution pattern of PCB exists.
Raulf
M
M
Institut Med. Mikrobiologie & Immunologie, AG Infektabwehrmechnismen, Ruhr-Universität Bochum, Germany.
König
W
W
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000097:blood; D066298:In Vitro Techniques; D010972:Platelet Activating Factor / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D012701:Serotonin / Q000097:blood
D006893:Hydroxyeicosatetraenoic Acids; D010972:Platelet Activating Factor; D012701:Serotonin; C068319:2,2',3,3'-tetrachlorobiphenyl; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; C029906:3,3'-dichlorobiphenyl; C009828:2,4,5,2',5'-pentachlorobiphenyl; D011078:Polychlorinated Biphenyls; C028451:3,4,3',4'-tetrachlorobiphenyl