title: Synthesis of a radioactive labeled undecagoldcluster for application in X-ray structure analysis of ribosomes.
abstract: In order to quantitate and to determine the extent of binding of a heavy atom derivative to ribosomal particles and other components of protein biosynthesis, we prepared a 14C-radioactive labeled monofunctional undecagoldcluster. This cluster can be bound either to exposed sulfhydryl groups at the surface of the ribosomes and its subunits, or to high nucleophilic amino groups of nucleic acids as well as to proteins.
authors.affiliation: Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin, F.R.G.
authors.forename: T
authors.initials: T
authors.lastname: Boeckh
authors.forename: H G
authors.initials: HG
authors.lastname: Wittmann
mesh_terms: D002250:Carbon Radioisotopes; D004591:Electrophoresis, Polyacrylamide Gel; D006046:Gold / Q000737:chemistry*; D007553:Isotope Labeling / Q000379:methods*; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D050607:Organogold Compounds; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000648:ultrastructure*; D014961:X-Ray Diffraction
chemical_list: D002250:Carbon Radioisotopes; D008024:Ligands; D050607:Organogold Compounds; D009942:Organometallic Compounds; D012269:Ribosomal Proteins; C035797:undecagold; D006046:Gold