Bacterial toxins induce heat shock proteins in human neutrophils.
We studied the influence of different bacterial toxins (alveolysin; toxic shock syndrome toxin 1, TSST-1 and erythrogenic toxin A, ETA) on the expression of heat shock proteins (hsps) in isolated human polymorphonuclear granulocytes (PMNs). As was shown by Western blotting (anti-hsp72) ETA and TSST-1 were potent inducers of hsps at low toxin concentrations (10 ng/ml). Alveolysin led to the expression of hsps at hemolytic concentrations (1 HU; 700 ng/ml) whereas at subhemolytic concentrations (7 ng/ml) no heat shock response was observed. The induction of heat shock proteins was also accompanied by increased mRNA levels for hsp70 as was determined by PCR-analysis.
Hensler
T
T
Lehrstuhl für Med. Mikrobiologie und Immunologie, Ruhr-Universität Bochum, F.R.G.
Köller
M
M
Alouf
J E
JE
König
W
W
D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000494:pharmacology*; D001483:Base Sequence; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000494:pharmacology; D005098:Exotoxins / Q000494:pharmacology; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000494:pharmacology; D006801:Humans; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000187:drug effects*; D009930:Organic Chemicals; D016133:Polymerase Chain Reaction; D011749:Pyrogens / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D013211:Staphylococcus aureus; D018089:Superantigens
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; D005098:Exotoxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D008565:Membrane Proteins; D009930:Organic Chemicals; D011749:Pyrogens; D012333:RNA, Messenger; C117980:SpeA protein, Streptococcus pyogenes; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal; C027951:erythrogenic toxin; C014963:alveolysin