Characterisation of a series of human immunodeficiency virus isolates derived sequentially from a single patient.
Five HIV-1 isolates were obtained sequentially from a single seropositive individual during the later stages of AIDS. Four of these isolates were adapted to grow in a continuous human T-lymphocytic cell line. Comparative biological and biochemical studies of the virus isolates were made using persistently infected cultures or virus derived from these systems respectively. The data obtained clearly shows that viruses with different biological properties can be isolated from the same individual at different times during the course of clinical AIDS.
Farrar
G H
GH
Division of Pathology, Public Health Laboratory Service, Centre for Applied Microbiology and Research, Salisbury, Wiltshire, United Kingdom.
Roff
M A
MA
Amin
T
T
Ball
J
J
Parrett
A M
AM
Battacharyya
U
U
Booth
J
J
Wansbrough-Jones
M H
MH
Greenaway
P J
PJ
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D002460:Cell Line; D004706:Endodeoxyribonucleases / Q000378:metabolism; D015726:Giant Cells; D015699:HIV Envelope Protein gp120 / Q000032:analysis / Q000737:chemistry; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D011533:Proviruses / Q000235:genetics; D012191:Retroviridae Proteins / Q000737:chemistry; D013601:T-Lymphocytes / Q000382:microbiology; D013997:Time Factors; D014779:Virus Replication
D015699:HIV Envelope Protein gp120; D012191:Retroviridae Proteins; D004706:Endodeoxyribonucleases