title: A new technique, requiring small amounts of cells, for the parallel study of chemiluminescence and phagocytosis via different receptors in the same cell population.
abstract: An assay permitting the parallel assessment of phagocytosis and chemiluminescence in the same cell population has been developed. The method is based on the phagocytosis of fluorescein isothiocyanate-conjugated yeast particles, either unopsonized or opsonized with complement factor C3 or IgG, by purified cells in suspension in a luminometer. Only a small number of cells (2 x 10(4)-1 x 10(5)) is required, and the reproducibility is high. Moreover, the technique permits phagocytosis to be related to oxygen-dependent killing activity in the same cell population. Since phagocytosis, degranulation and oxygen radical formation as a consequence of well-defined receptor recognition mechanisms can be characterized in very small cell populations, the method is suitable for monitoring the phagocytic function of cells from extravascular sites.
authors.affiliation: Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: C H
authors.initials: CH
authors.lastname: Sandgren
authors.forename: K
authors.initials: K
authors.lastname: Nordling
authors.forename: I
authors.initials: I
authors.lastname: Björk
mesh_terms: D000818:Animals; D002417:Cattle; D015550:Cell Degranulation; D003176:Complement C3 / Q000276:immunology; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005609:Free Radicals; D007074:Immunoglobulin G / Q000276:immunology; D008163:Luminescent Measurements; D009504:Neutrophils / Q000502:physiology*; D010100:Oxygen; D010587:Phagocytosis; D011951:Receptors, Complement / Q000502:physiology; D011961:Receptors, Fc / Q000502:physiology; D015203:Reproducibility of Results; D013861:Thiocyanates; D013997:Time Factors
chemical_list: D003176:Complement C3; D005452:Fluoresceins; D005609:Free Radicals; D007074:Immunoglobulin G; D011951:Receptors, Complement; D011961:Receptors, Fc; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate; D010100:Oxygen