Autoradiographic mapping of the glucose transporter with cytochalasin B in the mammalian eye.
The anatomic localization of the glucose transport protein in the eyes of rats, rabbits, baboons, marmosets, and humans with [3H]cytochalasin B using in vitro autoradiography showed high densities of glucose carrier densities. These densities were seen in the ciliary body, especially ciliary processes, iris, retina, and in some species, the trabecular meshwork and lens. In the lens, specific [3H]cytochalasin B-binding sites were mainly concentrated in the lens nucleus. Lower concentrations were found in the cortex. During aging, glucose transporter concentration increases up to the age of 8 yr in the marmoset lens nucleus, but decreases in the cortex and retina. Moderate amounts of carrier are located in the corneal endothelium and epithelium. The enrichment of glucose carrier protein in the trabecular meshwork suggests a high metabolic activity and a possible relationship in the regulation of intraocular pressure.
Kaulen
P
P
Department of Ophthalmology, Universitätsklinikum Rudolf Virchow, Standort Charlottenburg, Berlin, FRG.
Kahle
G
G
Keller
K
K
Wollensak
J
J
D000375:Aging / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D002143:Callitrichinae; D002924:Ciliary Body / Q000378:metabolism; D003571:Cytochalasin B / Q000378:metabolism*; D005123:Eye / Q000378:metabolism*; D006801:Humans; D007498:Iris / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D010215:Papio; D011817:Rabbits; D051381:Rats; D012160:Retina / Q000378:metabolism
D009004:Monosaccharide Transport Proteins; D003571:Cytochalasin B