title: Envelope alteration of Escherichia coli HB101 carrying pEAP31 caused by Kil peptide and its involvement in the extracellular release of periplasmic penicillinase from an alkaliphilic Bacillus.
abstract: The plasmid pEAP31 contains the colicin E1 kil gene. Peptidoglycan and outer-membrane components (lipopoly-saccharide, proteins and phosphatidylethanolamine) decreased concurrently in the envelope fraction from Escherichia coli HB101 carrying pEAP31 during the stationary phase of growth. At almost the same time. D-alanine residues in peptidoglycan decreased. The Kil peptide is suggested to affect, directly or indirectly, the turnover of peptidoglycan in stationary phase and, as a result, to cause partial exfoliation of the outer membrane. Periplasmic proteins are liberated from E. coli HB101 (pEAP31) probably because of the exfoliation of outer membrane.
authors.affiliation: Research Institute of Fermentation, Yamanashi University, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Aono
mesh_terms: D000409:Alanine / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D001409:Bacillus cereus / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000648:ultrastructure*; D029968:Escherichia coli Proteins; D008070:Lipopolysaccharides / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D010405:Penicillinase / Q000235:genetics / Q000378:metabolism*; D010457:Peptidoglycan / Q000032:analysis / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D010957:Plasmids
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D010457:Peptidoglycan; D010743:Phospholipids; C069003:kil protein, E coli; D010405:Penicillinase; D000409:Alanine