title: The YDp plasmids: a uniform set of vectors bearing versatile gene disruption cassettes for Saccharomyces cerevisiae.
abstract: The YDp plasmids (Yeast Disruption plasmids) are pUC9 vectors bearing a set of yeast gene disruption cassettes, all uniform in structure and differing only in the selectable marker used (HIS3, LEU2, LYS2, TRP1 or URA3). The markers, surrounded by translational termination codons, are embedded in the slightly modified sequence of the pUC9 multiple cloning sites.
authors.affiliation: Unité de Microbiologie, Faculté des Sciences Agronomiques de Gembloux, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Berben
authors.forename: J
authors.initials: J
authors.lastname: Dumont
authors.forename: V
authors.initials: V
authors.lastname: Gilliquet
authors.forename: P A
authors.initials: PA
authors.lastname: Bolle
authors.forename: F
authors.initials: F
authors.lastname: Hilger
mesh_terms: D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry; D005819:Genetic Markers; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D005819:Genetic Markers