Infection of SCID mice with lactate dehydrogenase-elevating virus stimulates B-cell activation.
Mice of the C.B-17 strain homozygous for the scid mutation (SCID mice) were infected with lactate dehydrogenase-elevating virus (LDV), and plasma samples obtained at intervals up to 42 days postinfection were analyzed for total immunoglobulins, anti-LDV antibodies, virus-specific immune complexes, and viremia levels. The mice responded to LDV infection with transient increases in total blood IgM, production of IgM-antigen complexes and IgM anti-LDV, as well as increased blood IgG2a. However, SCID mice failed to make a specific IgG2a anti-LDV immune response, and their blood LDV levels were elevated about 100-fold relative to those of control mice. The results suggest a role for IgG antibodies in the regulation of viremia and demonstrate a viral pathway of B-cell differentiation in SCID mice.
Bradley
D S
DS
Department of Microbiology, University of South Dakota School of Medicine, Vermillion.
Broen
J J
JJ
Cafruny
W A
WA
D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D015153:Blotting, Western; D015870:Gene Expression; D005803:Genes, Immunoglobulin / Q000276:immunology; D007136:Immunoglobulins / Q000032:analysis; D007371:Interferon-gamma / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D007772:Lactate dehydrogenase-elevating virus / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008817:Mice, Mutant Strains; D013154:Spleen / Q000378:metabolism; D014766:Viremia / Q000201:enzymology / Q000276:immunology*
D000936:Antigen-Antibody Complex; D007136:Immunoglobulins; D007371:Interferon-gamma; D007770:L-Lactate Dehydrogenase