title: Autonomous FSH synthesis in vitro in anterior pituitary glands and grafts from female rats.
abstract: When pituitary glands from intact female, but not from ovariectomized rats, are incubated for 8 h in medium TC199 without further additives, FSH is synthesized. This LHRH-independent (or autonomous) FSH synthesis is prevented when bovine follicular fluid (bFF) is added to the incubation medium. Results from preliminary experiments, however, indicate no clear autonomous FSH synthesis after long-term absence of LHRH. To investigate the regulatory mechanisms involved in autonomous FSH synthesis and release, pituitary glands (exposed to endogenous LHRH) and pituitary grafts (not exposed to endogenous LHRH) from intact and ovariectomized rats were incubated for 8 h in medium TC199. Total FSH content (FSH released plus FSH remaining in the tissue) was compared with that in non-incubated glands or grafts, giving an indication of FSH synthesis. In addition, some of the animals were given LHRH pulses for 40 h before incubation. When pituitary tissue was taken from intact female rats, FSH synthesis occurred in the animals' own glands and in grafts from LHRH-pretreated rats. No FSH synthesis was seen in ovariectomized rats with or without pretreatment with bFF and/or LHRH. However, when ovariectomized rats had been pretreated with oestrogen, FSH synthesis was measured in vitro after pulsatile LHRH treatment in vivo. The results indicate that autonomous FSH synthesis in vitro is dependent upon previous (in vivo) exposure of the glands to both oestrogen and LHRH.
authors.affiliation: Division of Medical Pharmacology, Sylvius Laboratory, University of Leiden, The Netherlands.
authors.forename: J A
authors.initials: JA
authors.lastname: van Dieten
authors.forename: J
authors.initials: J
authors.lastname: de Koning
authors.forename: G P
authors.initials: GP
authors.lastname: van Rees
mesh_terms: D000818:Animals; D004967:Estrogens / Q000494:pharmacology / Q000502:physiology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000096:biosynthesis*; D015571:Follicular Fluid / Q000502:physiology; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology / Q000502:physiology*; D009924:Organ Culture Techniques; D010052:Ovariectomy; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004967:Estrogens; D007987:Gonadotropin-Releasing Hormone; D005640:Follicle Stimulating Hormone