Stereospecific high-performance liquid chromatographic determination of tocainide.
A sensitive high-performance liquid chromatographic assay was developed for the determination of tocainide enantiomers in plasma. Following extraction of tocainide from plasma, the enantiomers were derivatized with S-(+)-1-(1-naphthyl)ethylisocyanate. The resulting diastereomers were separated and quantified using normal-phase chromatography with fluorescence detection set at 220/345 nm (excitation/emission). The peaks, resolved with a resolution factor greater than 1.5, were free from interference. Linearity was established over the concentration range 0.25-10.0 mg/l for each enantiomer in plasma (r2 greater than 0.998). The inter-assay variability was less than 10% at all concentrations examined. The method can be used to determine the pharmacokinetics of tocainide enantiomers in man.
Carr
R A
RA
Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Canada.
Foster
R T
RT
Freitag
D
D
Pasutto
F M
FM
D000328:Adult; D000889:Anti-Arrhythmia Agents / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003485:Cyanates; D006801:Humans; D017953:Isocyanates; D008012:Lidocaine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D008297:Male; D009281:Naphthalenes; D013237:Stereoisomerism; D016677:Tocainide
D000889:Anti-Arrhythmia Agents; D003485:Cyanates; D017953:Isocyanates; D009281:Naphthalenes; D016677:Tocainide; C054308:1-(1-naphthyl)ethyl isocyanate; D008012:Lidocaine