Characterization of eugonic fermenters group EF-4 by polyacrylamide gel electrophoresis and protein immunoblot analysis.
Whole-cell lysates and proteinase K-extracted lipopolysaccharide (LPS) of 19 strains of the group eugonic fermenter-4 (EF-4) were analyzed by electrophoresis and protein immunoblotting. These strains were isolated from dog- and cat-bite abscesses in human beings, ferret and human gastric lesions, and cat-lung infections. These strains represent 2 biovar groupings; EF-4a biovars ferment glucose and possess arginine dihydrolase activity, whereas EF-4b biovars do not. Electrophoresis of whole-cell lysates could distinguish between these biovars groups. Electrophoresis of LPS extracts revealed that all strains of EF-4 possess smooth chemotypes. Two strains of EF-4a reacted weekly in protein immunoblots and revealed distinct LPS profiles. These studies suggests that subgroups of EF-4 biovars may exist.
Hanner
T L
TL
Department of Laboratory Animal Science, North Carolina Agricultural and Technical State University, Greensboro 27411.
Allen
J W
JW
Robertson-Byers
A
A
Hurley
S L
SL
D000818:Animals; D001424:Bacterial Infections / Q000382:microbiology*; D001426:Bacterial Proteins / Q000032:analysis*; D001733:Bites and Stings / Q000382:microbiology; D002371:Cat Diseases / Q000382:microbiology; D002415:Cats; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D005289:Ferrets; D006090:Gram-Negative Bacteria / Q000032:analysis*; D006801:Humans; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000032:analysis*; D008171:Lung Diseases / Q000382:microbiology / Q000662:veterinary; D013276:Stomach Ulcer / Q000382:microbiology / Q000662:veterinary
D001426:Bacterial Proteins; D008070:Lipopolysaccharides