[Molecular biological study of the vaccinia virus genome. IV. The late nonstructural 36K protein of vaccinia virus is vitally important].
Two genetics markers: the herpes simplex virus thymidine kinase and Escherichia coli beta-galactosidase genes were inserted into the 36K protein gene of vaccinia virus located in a HindIII-P DNA fragment. An unstability of recombinant viruses with Lac(+)-phenotype were discovered. A mechanism of viruses unstable variants formation was proposed, it was confirmed by the results of hybridisation analyses of virus recombinant genomes. The importance of a late nonstructural 36K protein gene for virus reproduction was demonstrated.
Shchelkunov
S N
SN
Riazankina
O I
OI
Gashnikov
P B
PB
Totmenin
A B
AB
Chizhikov
V E
VE
Malygin
E G
EG
D001345:Autoradiography; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology; D005814:Genes, Viral; D005819:Genetic Markers; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010957:Plasmids; D011995:Recombination, Genetic; D013937:Thymidine Kinase / Q000235:genetics; D014616:Vaccinia virus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
D005819:Genetic Markers; D014764:Viral Proteins; D013937:Thymidine Kinase; D001616:beta-Galactosidase
Molekuliarno-biologicheskoe izuchenie genoma virusa ospovaktsiny. IV Gen pozdnego nestrukturnogo belka 36K virusa ospovaktsiny iavliaetsia zhiznenno vazhnym.