[SDS-PAGE analysis of surface proteins and antigens evidences high heterogenity in natural clones of Trypanosoma cruzi, correlated with isoenzyme variability].
Surface protein and surface antigen patterns of 19 Trypanosoma cruzi laboratory clones, representing 17 different isozymic profiles (zymodemes), were compared by SDS-PAGE analysis. Surface protein patterns were found to be complex and heterogeneous. According to the number of common bands, we calculated similarity coefficients of surface protein patterns on the one hand, and of surface antigen patterns on the other hand, for 33 stock pairwise comparisons. In both cases, these coefficients were statistically correlated to the isozyme index of genetic identity. Such a correlation between independent genetic markers favours the clonal structure of T. cruzi natural populations previously evidenced. Moreover, we did not observe any notable differences in the surface antigen pattern among 4 T. cruzi cloned stocks precipitated by homologous as well as heterologous hyperimmune sera. The immunological significance of the molecular weight variability in surface antigen patterns among different zymodemes is discussed.
Brenière
S F
SF
Laboratoire de Génétique des Parasites et des Vecteurs, ORSTOM, Montpellier.
Araniki
I
I
Le Ray
D
D
Tibayrenc
M
M
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D002999:Clone Cells / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry*; D014349:Trypanosoma cruzi / Q000032:analysis* / Q000166:cytology / Q000201:enzymology / Q000276:immunology
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D007527:Isoenzymes; D008565:Membrane Proteins
L'analyse SDS-PAGE des protéines et antigènes de surface révèle une forte hétérogénéité chez les clones naturels de Trypanosoma cruzi, corrélée à la variabilité isoenzymatique.