Primary structure and expression of bovine poly(A) polymerase.
Poly(A) polymerase has a critical role in the synthesis of messenger RNA in eukaryotic cells. The isolation and characterization of complementary DNAs encoding bovine poly(A) polymerase is described here. The predicted sequences of the mRNA and protein reveal features that provide insights into how the enzyme functions and how it might be regulated. Poly(A) polymerase expressed from a cloned cDNA is fully functional in in vitro assays, and mutational analyses have identified a putative regulatory domain that enhances, but is not essential for, activity.
Raabe
T
T
Department of Biological Sciences, Columbia University, New York, New York 10027.
Bollum
F J
FJ
Manley
J L
JL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D015870:Gene Expression; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011062:Polynucleotide Adenylyltransferase / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D011062:Polynucleotide Adenylyltransferase