[Identification of twenty-eight species mycobacteria with their cellular fatty acids by capillary gas chromatography].
Cellular fatty acid (FA) of 28 species of mycobacteria were analysed by capillary gas chromatography (GC). There were more than sixty volatile FA before C12:0 have no any difference between species both in quality and quantity. The identification scheme of Mycobacterium based on cellular FA was presented here. According to this scheme, all the tested species could be divided into 8 groups by the FA peak height order of C18:1, C18:0 and TBSA which were quite stable; after that, 17 species (60%) might be identified to species level with help of some distinctive FA such as C19:0, C21:0. Most pathogenic bacteria could be separated by the scheme (M. tuberculosis, M. bovis and some complexes). This scheme was also demonstrated to be objective rationality by computer cluster analysis with cellular FA components.
Zhang
Y
Y
Institute of Microbiology, Academia Sinica, Beijing.
Zhuang
Y
Y
Liu
Z
Z
Ruan
J
J
D002849:Chromatography, Gas / Q000379:methods; D016000:Cluster Analysis; D005227:Fatty Acids / Q000032:analysis*; D009161:Mycobacterium / Q000032:analysis / Q000302:isolation & purification*; D009163:Mycobacterium bovis / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D013045:Species Specificity
D005227:Fatty Acids