title: Regulation of gene expression and cellular localization of prostaglandin synthase by oestrogen and progesterone in the ovine uterus.
abstract: Expression of the gene for prostaglandin synthase (PGS) was examined in whole endometrial tissue derived from ewes during the oestrous cycle (Days 4-14), on Day 15 of pregnancy and following ovariectomy and treatment with ovarian steroid hormones. Whilst no significant differences were seen in PGS mRNA concentrations analysed by Northern blot analysis in endometrial tissue during the oestrous cycle or in early pregnancy, treatment of ovariectomized (OVX) ewes with oestradiol-17 beta markedly reduced endometrial PGS mRNA concentration. There was no difference in PGS mRNA concentration in ewes treated with progesterone, either alone or in conjunction with oestrogen, from that in OVX controls. In contrast, differences in immunolocalization of PGS observed in uterine tissue from OVX-steroid-treated ewes were much more marked and reflected similar changes seen previously in the immunocytochemical distribution of endometrial PGS during the oestrous cycle. In OVX ewes and those treated with oestrogen, immunocytochemical staining for PGS was seen in stromal cells, but little immunoreactive PGS was located in the endometrial epithelial cells. However, in ewes treated with progesterone alone or with oestrogen plus progesterone, PGS was found in luminal and glandular epithelial cells and in stromal cells. Intensity of immunostaining for PGS in endothelial cells and myometrium did not differ between the treatments. Thus, whilst oestrogen lowers PGS mRNA in the endometrium, presumably in stroma, it may also increase the stability of the enzyme itself in the stromal cells. Although oestradiol-17 beta has no effect on PGS in endometrial epithelium, progesterone stimulates the production of PGS in endometrial epithelial cells without altering the overall abundance of PGS mRNA in the endometrium as a whole. Conceptus-induced changes in PGF-2 alpha release by ovine endometrium would not appear to be mediated via effects on PGS gene expression or protein synthesis.
authors.affiliation: Prince Henry's Institute of Medical Research, South Melbourne, Vic., Australia.
authors.forename: L A
authors.initials: LA
authors.lastname: Salamonsen
authors.forename: A L
authors.initials: AL
authors.lastname: Hampton
authors.forename: J A
authors.initials: JA
authors.lastname: Clements
authors.forename: J K
authors.initials: JK
authors.lastname: Findlay
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D004958:Estradiol / Q000494:pharmacology / Q000502:physiology*; D004971:Estrus / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D010052:Ovariectomy; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology / Q000502:physiology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D012756:Sheep / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; D011374:Progesterone; D004958:Estradiol; D011451:Prostaglandin-Endoperoxide Synthases