A vector for analysis of promoters in the cyanobacterium Anabaena sp. strain PCC 7120.
A plasmid vector containing a multiple-cloning site followed by a promoterless chloramphenicol acetyltransferase (cat) gene, protected by transcription terminators and mobilizable by conjugation from Escherichia coli into Anabaena sp. strain PCC 7120, was constructed. The utility of the vector was shown by deletion analysis of the promoter region of the Anabaena psbB gene.
Lang
J D
JD
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Haselkorn
R
R
D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000235:genetics*; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*