title: Cold preservation injury of NADH-ubiquinone oxidoreductase.
abstract: Mitochondrial injury caused by cold preservation without oxygenation was studied polarographically. Respiration activity with glutamate as substrate was impaired after 6 hours preservation with Euro-Collin's solution at 4 degrees C, while that with succinate as substrate was maintained at the control level after 24 hours preservation. Membrane potential across mitochondrial membrane in state 4 was not damaged after 24 hours preservation. These results indicate that NADH-ubiquinone oxidoreductase is impaired during cold and simple preservation.
authors.affiliation: Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
authors.forename: G Y
authors.initials: GY
authors.lastname: Meng
authors.forename: A
authors.initials: A
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Ozawa
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism; D003080:Cold Temperature; D015925:Cryopreservation; D007425:Intracellular Membranes / Q000502:physiology; D008099:Liver / Q000166:cytology / Q000201:enzymology; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D008930:Mitochondria, Liver / Q000201:enzymology* / Q000473:pathology; D016660:NAD(P)H Dehydrogenase (Quinone); D010085:Oxidative Phosphorylation; D010619:Phenazines / Q000378:metabolism; D011808:Quinone Reductases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry / Q000379:methods
chemical_list: D000970:Antineoplastic Agents; D010619:Phenazines; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases; C009195:safranine T