Interactions of 5-hydroxytryptamine with oxidative enzymes.
Peroxidase (EC 1.11.1.7)/H2O2, ceruloplasmin (human type X)/O2, and tyrosinase (EC 1.14.18.1)/O2 all oxidized the indolic neurotransmitter 5-hydroxytryptamine (5-HT) in the physiological pH domain. Peroxidase/H2O2 oxidized 5-HT at pH values down to about 2.5. All oxidation reactions generated complex mixtures of products which included at least one known neurotoxin, tryptamine-4,5-dione. In general, the enzymatic oxidation pathways paralleled the in vitro electrochemical oxidation of 5-HT which has permitted suggestions to be made concerning the probable mechanisms of the enzyme-mediated reactions.
Wrona
M Z
MZ
Department of Chemistry and Biochemistry, University of Oklahoma, Norman 73019.
Dryhurst
G
G
D002570:Ceruloplasmin / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*; D012701:Serotonin / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*
D012701:Serotonin; D010544:Peroxidases; D014442:Monophenol Monooxygenase; D002570:Ceruloplasmin