Alterations in the accumulation of adenylylated nucleotides in heavy-metal-ion-stressed and heat-stressed Synechococcus sp. strain PCC 6301, a cyanobacterium, in light and dark.
Heavy-metal-ion- (Cd2+, Cu2+, Pb2+, Hg2+ and Zn2+) or heat (50 degrees C)-stress treatments of the unicellular cyanobacterium Synechococcus sp., strain PCC 6301, under both light and dark conditions led to the accumulation of bis(5'-nucleosidyl)oligophosphates: Ap4A, Ap4G, Ap3A, Ap3G and Ap3Gp2. Under light regimens, the accumulation of Ap4A and Ap4G is more characteristic of heavy-metal-ion-stressed cells, whereas the accumulation of Ap3A, Ap3G and Ap3Gp2 is the dominant feature of heavy-metal-ion or heat-shock treatment during energy deprivation (i.e. in the dark). This accumulation of bisnucleoside oligophosphates supports a model whereby the adenylylated nucleotides are synthesized by the backward reaction of tRNA-aminoacyl synthetases. These nucleotides may also act to switch or modulate cyanobacterial responses under various environmental stress conditions.
Pálfi
Z
Z
Institute of Plant Physiology, Biological Research Center, Szeged, Hungary.
Surányi
G
G
Borbély
G
G
D000227:Adenine Nucleotides / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D002104:Cadmium / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D000458:Cyanobacteria / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D003624:Darkness; D006358:Hot Temperature; D007700:Kinetics; D007854:Lead / Q000494:pharmacology; D008027:Light; D008628:Mercury / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology*; D015032:Zinc / Q000494:pharmacology
D000227:Adenine Nucleotides; D008670:Metals; D002104:Cadmium; D007854:Lead; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D003300:Copper; D000255:Adenosine Triphosphate; D008628:Mercury; D015032:Zinc