Identity of hemolysins produced by Bacillus thuringiensis and Bacillus cereus.
A hemolysin (Bt-hemolysin) produced by Bacillus thuringiensis var. kurstaki HD-1 producing crystalline toxin(s) was purified by successive treatments of ammonium sulfate (45-65%) and column chromatography using DEAE-cellulose, Sephadex G-75 and KB-002 (a hydroxyapatite column for fast protein liquid chromatography). A hemolysin (Bc-hemolysin) produced by B. cereus HG-6A was also purified by the same procedure. The purified Bt-hemolysin and Bc-hemolysin, both of which are thiol-activated hemolysins, were biologically, physicochemically and immunologically identical. These findings provide further evidence of the similarity of B. thuringiensis, which is being used as a biological insecticide, to B. cereus, a toxigenic organism of food poisoning.
Honda
T
T
Research Institute for Microbial Diseases, Osaka University, Japan.
Shiba
A
A
Seo
S
S
Yamamoto
J
J
Matsuyama
J
J
Miwatani
T
T
D001409:Bacillus cereus / Q000032:analysis* / Q000472:pathogenicity; D001413:Bacillus thuringiensis / Q000032:analysis* / Q000472:pathogenicity; D004229:Dithiothreitol / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005517:Foodborne Diseases / Q000382:microbiology; D006460:Hemolysin Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D006461:Hemolysis; D006358:Hot Temperature; D006801:Humans; D005779:Immunodiffusion; D007564:Japan
D006460:Hemolysin Proteins; D004229:Dithiothreitol