title: Sulfonylureas activate glucose transport and protein kinase C in rat adipocytes.
abstract: Glyburide and tolbutamide, at concentrations of 20 to 40 mumol/L and 1 to 2 mmol/L, respectively, stimulated glucose transport in rat adipocytes. Concomitantly, protein kinase C was activated, as evidenced by translocation of immunoreactive enzyme from cytosol to membranes. Glucose transport effects of the sulfonylureas were blocked by three inhibitors of protein kinase C (H-7, staurosporine, and sangivamycin), and by phorbol ester-induced down-regulation of protein kinase C. These findings suggest that sulfonylureas may stimulate glucose transport in rat adipocytes through activation of protein kinase C.
authors.affiliation: Veterans' Administration Hospital, Tampa, FL 33612.
authors.forename: R V
authors.initials: RV
authors.lastname: Farese
authors.forename: T
authors.initials: T
authors.lastname: Ishizuka
authors.forename: M L
authors.initials: ML
authors.lastname: Standaert
authors.forename: D R
authors.initials: DR
authors.lastname: Cooper
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000201:enzymology*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003847:Deoxyglucose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005905:Glyburide / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008297:Male; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013453:Sulfonylurea Compounds / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014044:Tolbutamide / Q000494:pharmacology
chemical_list: D007328:Insulin; D013453:Sulfonylurea Compounds; D014044:Tolbutamide; D003847:Deoxyglucose; D011493:Protein Kinase C; D005947:Glucose; D013755:Tetradecanoylphorbol Acetate; D005905:Glyburide