Radioprotection of whole-body gamma-irradiation-induced alteration in some haematological parameters by cysteine, vitamin E and their combination in rats.
Radioprotective effect of cysteine, vitamin E and their combination on gamma-irradiation-induced alteration in some haematological parameters in male rats has been studied 24 and 48 hrs after whole-body gamma-irradiation at a dose level of 7.5 Gy. The results of this study reveal that gamma-irradiation caused a significant decrease in red blood cells (RBCs) count with insignificant change in hemoglobin level, 24 and 48 hrs postirradiation, gamma-irradiated rats showed as well a progressive decrease in their blood ATP, and serum-SH levels with a significant increase in blood glutathione (GSH) level. Administration of cysteine or vitamin E preceding gamma-radiation exposure gave a significant radioprotection to the above haematological parameters. However, combination of both agents afforded a better protection, so that most of the measured parameters were restored to the pre-irradiated values. Finally, the date demonstrate that the radioprotection provided by combined administration of vitamin E and cysteine is feasible and perhaps, even more efficient against radiation injury to RBCs. This will appreciate the usage of such combination in protecting the patient during radiotherapy.
Shaheen
A A
AA
Biochemistry Department, Faculty of Pharmacy, Cairo University, Egypt.
Hassan
S M
SM
D000818:Animals; D001769:Blood / Q000187:drug effects* / Q000528:radiation effects*; D003545:Cysteine / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004359:Drug Therapy, Combination; D004906:Erythrocyte Count / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays / Q000009:adverse effects; D005978:Glutathione / Q000097:blood / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D011833:Radiation Injuries, Experimental / Q000097:blood / Q000517:prevention & control; D011837:Radiation-Protective Agents; D051381:Rats; D011919:Rats, Inbred Strains; D013438:Sulfhydryl Compounds / Q000097:blood / Q000528:radiation effects; D013997:Time Factors; D014810:Vitamin E / Q000627:therapeutic use*; D014916:Whole-Body Irradiation / Q000009:adverse effects*
D011837:Radiation-Protective Agents; D013438:Sulfhydryl Compounds; D014810:Vitamin E; D005978:Glutathione; D003545:Cysteine