Identification of endogenous sugar-binding proteins in the accessory sex glands of NMRI mice. A histochemical and biochemical study.
In the present study we report on the histotopographical distribution of carbohydrate-binding proteins in the prostate and seminal vesicle of sexually mature NMRI mice using a panel of fluorescein-isothiocyanate labelled neoglycoproteins and asialoglycoproteins. Additionally, biochemical analysis using affinity chromatography and SDS-gel electrophoresis was performed to purify and characterize the respective proteins from the tissue. Our histochemical results clearly demonstrate the presence of endogenous receptors for the carbohydrate part of glycoconjugates in both glands. In the prostate a distinct staining was seen after incubation with melibiose-BSA-FTC, glucuronic acid-BSA-FTC and asialofetuin-FTC (only in the ventral prostate). In the epithelium of the seminal vesicle a weak staining occurred after incubation with asialofetuin-FTC and maltose-FTC. In the stroma of both accessory sex glands a distinct binding of several (neo)glycoproteins specific for beta-galactoside-binding proteins was observed which could be attributed to a beta-galactoside-binding lectin. Indeed biochemical analysis ascertained presence of such a histochemically detectable activity. We assume that the carbohydrate-binding proteins of the stroma, which were obviously linked to the elastic fibers, could play a role in the organisation of the extracellular matrix in the interstitium of the glands.
Sinowatz
F
F
Institute of Veterinary Anatomy, University of Munich, FRG.
Gabius
H J
HJ
Hauke
C
C
Breipohl
W
W
Amselgruber
W
W
D000818:Animals; D001212:Asialoglycoproteins / Q000378:metabolism; D002352:Carrier Proteins / Q000032:analysis*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005109:Extracellular Matrix; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006023:Glycoproteins / Q000378:metabolism; D006651:Histocytochemistry; D037102:Lectins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011467:Prostate / Q000737:chemistry* / Q000166:cytology; D011956:Receptors, Cell Surface; D012669:Seminal Vesicles / Q000737:chemistry* / Q000166:cytology; D013861:Thiocyanates
D001212:Asialoglycoproteins; D002352:Carrier Proteins; D005452:Fluoresceins; D006023:Glycoproteins; D037102:Lectins; D011956:Receptors, Cell Surface; D013861:Thiocyanates; C055213:saccharide-binding proteins; D016650:Fluorescein-5-isothiocyanate