Analysis of the spontaneous in vitro anti-HIV-1 antibody secretion by peripheral blood mononuclear cells in HIV-1 infection.
We studied the spontaneous in vitro secretion of anti-HIV-1 antibodies by peripheral blood mononuclear cells (PBMC) from HIV-1-infected patients. Specific antibody production was detected in supernatants of PBMC cultures using an ELISA; HIV-1 specificity was confirmed by antigen adsorption and Western blotting. This antibody secretion was found to be an active phenomenon and was not due to a release of plasma antibodies passively adsorbed onto the cell membranes. In all positive supernatants, anti-HIV-1-secreted antibodies were directed against env-encoded antigens and many supernatants also contained antibodies to pol- and gag-encoded antigens. PBMC from all HIV-1-infected patients tested (140 adults and 18 infants) secreted anti-HIV-1 antibodies. This production was found during all the clinical stages of HIV-1 infection. Our results suggest that this spontaneous HIV-1-specific antibody secretion represents a marker of HIV-1 infection. Detection of these antibodies could be a valuable tool for early confirmation of HIV-1 infection in neonates born to HIV-1-seropositive mothers.
Vendrell
J P
JP
Institut National de la Santè et la Recherche Médicale (INSERM), Unité 249, Institut de Biologie, Montpellier, France.
Segondy
M
M
Ducos
J
J
Reynes
J
J
Huguet
M F
MF
Nicolas
J C
JC
Serre
A
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D015683:Gene Products, gag / Q000032:analysis; D015483:HIV Antibodies / Q000032:analysis*; D016655:HIV Core Protein p24; D015658:HIV Infections / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008875:Middle Aged; D014758:Viral Core Proteins / Q000032:analysis
D015683:Gene Products, gag; D015483:HIV Antibodies; D016655:HIV Core Protein p24; D014758:Viral Core Proteins