title: Purification of a hybrid plasminogen activator protein.
abstract: Recombinant DNA technology has been employed to produce a hybrid gene in which the kringle and serine protease domains of tissue plasminogen activator are linked to the heavy-chain Fd region of a fibrin-specific antibody. The hybrid gene is co-expressed with antibody light chains. This communication describes a purification procedure for the hybrid protein, involving affinity and ion-exchange chromatography. The purified hybrid protein has been used in vivo and in vitro clot lysis experiments and has been shown to be effective at clot dissolution.
authors.affiliation: Celltech Limited, Slough, Berks, U.K.
authors.forename: J
authors.initials: J
authors.lastname: Deistung
authors.forename: D M
authors.initials: DM
authors.lastname: Forde
authors.forename: J P
authors.initials: JP
authors.lastname: O'Connell
authors.forename: K A
authors.initials: KA
authors.lastname: Proudfoot
authors.forename: D
authors.initials: D
authors.lastname: Eaton
authors.forename: F
authors.initials: F
authors.lastname: Willenbrock
authors.forename: R O
authors.initials: RO
authors.lastname: Kingaby
authors.forename: B
authors.initials: B
authors.lastname: Hughes
authors.forename: S
authors.initials: S
authors.lastname: Angal
authors.forename: C
authors.initials: C
authors.lastname: Catterall
mesh_terms: D000818:Animals; D001777:Blood Coagulation; D002478:Cells, Cultured; D002846:Chromatography, Affinity / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005337:Fibrin / Q000276:immunology; D006824:Hybridization, Genetic / Q000235:genetics*; D010053:Ovary / Q000737:chemistry / Q000166:cytology; D011995:Recombination, Genetic; D010959:Tissue Plasminogen Activator / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D005337:Fibrin; D010959:Tissue Plasminogen Activator