Germline transcripts of the immunoglobulin heavy-chain and T cell receptor genes in a murine hematopoietic stem cell line LyD9 and its derivative cell lines.
We compared germline transcript levels of immunoglobulin heavy chain and T cell receptor (TcR) genes in a murine hematopoietic stem cell line, LyD9, and its derivative cell lines. LyD9 cells can be induced to differentiate into at least three lineages, namely, B lymphocyte, macrophage, and granulocyte lineages. Although C mu transcripts were found in stem cells to B lymphocytes, other myeloid-committed cells also expressed significant amounts of C mu transcripts. Germline TcR transcripts did not show good correlation with differentiation potential and stages of hematopoietic cells. During this search we identified a novel germline transcript containing the JH-C microliter sequence in LyD9 and some of its derivative cells. Expression of mRNAs for immunoglobulin- and TcR-associated molecules (lambda 5, MB1 and CD3 delta) was widespread except for lambda 5 mRNA. Among three mRNAs encoding putative recombinase proteins, RAG-1 and RAG-2 mRNAs were not expressed in any cell lines tested, while RBP-2 mRNA was expressed ubiquitously.
Tashiro
K
K
Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.
Kinashi
T
T
Anderson
S J
SJ
Lee
K H
KH
Tohyama
K
K
Ogawa
M
M
Takahashi
M
M
Kawaichi
M
M
Honjo
T
T
D000818:Animals; D001402:B-Lymphocytes; D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004254:DNA Nucleotidyltransferases / Q000235:genetics; D005803:Genes, Immunoglobulin; D006098:Granulocytes; D006133:Growth Substances / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000378:metabolism*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D019426:Integrases; D008264:Macrophages; D051379:Mice; D008808:Mice, Inbred CBA / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D045522:Recombinases
D006133:Growth Substances; D007143:Immunoglobulin Heavy Chains; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D045522:Recombinases; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C115006:integron integrase IntI1