Transcriptional and posttranscriptional regulation of class I major histocompatibility complex genes following transformation with human adenoviruses.
Transformation of rodent cells by human adenoviruses is a well-established model system for studying the expression, regulation, and function of class I antigens. In this report, we demonstrate that the highly oncogenic adenovirus type 12 operates at the transcriptional and posttranscriptional levels in regulating the activity of major histocompatibility complex class I genes and products in transformed cells. Adenovirus type 12 suppresses the cell surface expression of class I antigens in most cell lines. Nevertheless, in a number of cell lines suppression is the result of reduction in the amount of stable specific mRNA, while in another group of cell lines suppression involves interference with processing of a posttranscriptional product. The two mechanisms operate both for the endogenous H-2 genes and for a miniature swine class I transgene that is expressed in the cells.
Shemesh
J
J
Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
Rotem-Yehudar
R
R
Ehrlich
R
R
D000260:Adenoviruses, Human / Q000235:genetics* / Q000276:immunology; D000818:Animals; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D005805:Genes, MHC Class I; D006183:H-2 Antigens / Q000032:analysis / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D013489:Suppression, Genetic; D013552:Swine; D013556:Swine, Miniature; D014158:Transcription, Genetic
D006183:H-2 Antigens