B subunit of cholera toxin produced in Escherichia coli.
An engineered E. coli strain containing high expression level of CT-B subunits has been obtained by the application of recombinant DNA techniques. The B subunit can be secreted into the medium and reaches 20-40 micrograms/ml when this strain is incubated in a 50 l fermentation tank. The CT-B subunit purified with affinity chromatography in E. coli has the same characters as the natural CT-B subunit in molecular weight, N terminal amino acid analysis and antigenicity. The CT-B subunit has good immunogenicity and can be used as a preparation for protecting against diarrhea caused by V. cholera and enterotoxigenic E. coli. It can also be used as a vector for hepatins.
Ma
Q J
QJ
Bioengineering Institute, Academy of Military Medical Sciences, Beijing, PRC.
Liu
C X
CX
Xiong
L S
LS
Yu
X Q
XQ
D000818:Animals; D002771:Cholera / Q000517:prevention & control; D002772:Cholera Toxin / Q000096:biosynthesis* / Q000276:immunology; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000502:physiology*; D005818:Genetic Engineering; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008297:Male; D010446:Peptide Fragments / Q000096:biosynthesis* / Q000276:immunology; D011817:Rabbits; D014611:Vaccination
D004274:DNA, Recombinant; D010446:Peptide Fragments; C050310:cholera toxin, B subunit (50-64); D002772:Cholera Toxin