Bispecific antibodies that mediate killing of cells infected with human immunodeficiency virus of any strain.
Although AIDS patients lose human immunodeficiency virus (HIV)-specific cytotoxic T cells, their remaining CD8-positive T lymphocytes maintain cytotoxic function. To exploit this fact we have constructed bispecific antibodies that direct cytotoxic T lymphocytes of any specificity to cells that express gp120 of HIV. These bispecific antibodies comprise one heavy/light chain pair from an antibody to CD3, linked to a heavy chain whose variable region has been replaced with sequences from CD4 plus a second light chain. CD3 is part of the antigen receptor on T cells and is responsible for signal transduction. In the presence of these bispecific antibodies, T cells of irrelevant specificity effectively lyse HIV-infected cells in vitro.
Berg
J
J
Department of Microbiology and Immunology, University of California, San Francisco 94143-0414.
Lötscher
E
E
Steimer
K S
KS
Capon
D J
DJ
Baenziger
J
J
Jäck
H M
HM
Wabl
M
M
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D015704:CD4 Antigens / Q000235:genetics / Q000276:immunology*; D016827:CD8 Antigens; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D005455:Fluorescent Antibody Technique; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies / Q000276:immunology*; D006367:HeLa Cells / Q000276:immunology; D006801:Humans; D010957:Plasmids; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D015704:CD4 Antigens; D016827:CD8 Antigens; D015483:HIV Antibodies