Synergism between electricity and ionizing radiation.
Weak direct electric currents which produce little (or no) lethal damage to Escherichia coli bacteria are shown to act synergistically with ionizing radiation, both electromagnetic radiation (X-ray) and charged particles (beta radiation). This synergism greatly enhances the lethal effect of ionizing radiation on bacteria. This is possibly due to increased single-strand breaks in DNA, as detected by the alkaline sucrose gradient method. It is also shown that in cells with thymidine-3H incorporated into their DNA and treated with electricity, the radioactivity is released from the acid-insoluble fraction to the acid-soluble fraction, so that the ratio of radioactivity in the soluble fraction to that in the insoluble fraction increases from 0.47 in the non-treated control cells to 3.46 in the cells treated with an electric current of 1.0 mA (3.0 V) for 30 min, which indicates extensive degradation of cellular DNA. No synergism is detected between electricity and 254 nm UV radiation nor between electricity and X-rays, when these two agents are used sequentially in any order. Electricity alone produces lesions in cell membranes, as shown by electron microscopy.
Capella
M A
MA
Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, UFRJ, Rio de Janeiro, Brazil.
Fonseca
M E
ME
Menezes
S
S
D001610:Beta Particles; D004249:DNA Damage; D004261:DNA Replication / Q000528:radiation effects; D004269:DNA, Bacterial / Q000235:genetics / Q000528:radiation effects; D004560:Electricity; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects* / Q000648:ultrastructure; D007700:Kinetics; D008854:Microscopy, Electron; D014466:Ultraviolet Rays; D014965:X-Rays
D004269:DNA, Bacterial