Automated aflatoxin analysis of foods and animal feeds using immunoaffinity column clean-up and high-performance liquid chromatographic determination.
A commercially available system is described for the fully automated clean-up and high-performance liquid chromatographic (HPLC) analysis of aflatoxins in foods and animal feeds. The system marketed primarily for handling solid-phase extraction columns has modified software to facilitate use with immunoaffinity columns. Sample extract clean-up followed by injection onto an HPLC column with post-column iodination and fluorescence detection is carried out completely unattended. A coefficient of variation of 5.1% for aflatoxin B1 analysis was obtained, and the accuracy of the system was demonstrated by the analysis of peanut butter certified reference material.
Sharman
M
M
Ministry of Agriculture, Fisheries and Food, Food Science Laboratory, Norwich, U.K.
Gilbert
J
J
D000348:Aflatoxins / Q000032:analysis* / Q000276:immunology; D000821:Animal Feed / Q000032:analysis*; D010367:Arachis / Q000032:analysis; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis; D005506:Food Contamination; D005638:Fruit / Q000032:analysis; D007120:Immunochemistry; D009754:Nuts / Q000032:analysis; D011786:Quality Control
D000348:Aflatoxins