Utilization of human cultured epidermal keratinocytes: irreversibility of the inhibition of proliferation induced in stored detached cultures.
In order to look for the best conditions required for the utilization of cultured epithelium in the treatment of burn wounds, some experiments have been performed studying the storage of dispase-detached cultures. The viability of keratinocytes and, after trypsin dissociation of the cultures, the adhesion and proliferative potential of living keratinocytes were measured. Our laboratory investigations suggest that the storage period of detached cultures has to be kept as short as possible to preserve the keratinocytes' growth potential.
Boucher
F
F
Department of Histology-Embryology, Facultés Universitaires Notre-Dame de la Paix, Namur, Belgium.
Poumay
Y
Y
Degen
A
A
Paye
M
M
Leloup
R
R
D002056:Burns / Q000601:surgery*; D002448:Cell Adhesion / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D006801:Humans; D015603:Keratinocytes / Q000637:transplantation*; D012867:Skin / Q000166:cytology; D016038:Skin Transplantation / Q000379:methods; D014021:Tissue Preservation