Two cationic amino acid transport systems in human placental basal plasma membranes.
Transport of cationic amino acids in basal (fetal facing) plasma membranes was investigated by characterization of L-[3H]lysine and L-[3H]arginine uptake in membrane vesicles isolated from term human placentas. At least two Na(+)-independent systems were present. Lysine concentration dependence data were fit by a two-system model with Km values of 1.0 +/- 0.8 and 223 +/- 57 microM and Vmax values of 0.06 +/- 0.03 and 24.0 +/- 5.8 pmol.mg protein-1.min-1. In the presence of either 10 mM L-leucine or Na+ plus 10 mM L-homoserine, the data were fit by single system models with kinetic parameters similar to the higher and lower Km systems seen in the absence of inhibitors. Uptake of 10 or 20 microM L-lysine in the absence of Na+ showed the higher Km system was inhibited completely by L-arginine, L-homoarginine, and L-histidine. In the presence of Na+, the higher Km system was inhibited completely by L-alanine, L-homoserine, L-leucine, L-phenylalanine, and L-norleucine. The lower Km system was inhibited completely by L-arginine, L-homoarginine, L-histidine, L-leucine, and L-methionine. Time course studies of uptake demonstrated that uptake by either system alone filled the total vesicular space. The basal membrane of human placental syncytiotrophoblast possesses two transport systems for lysine and arginine, resembling the ubiquitous y+ system and the bo,+ system previously described in mouse blastocysts. The higher Vmax of the y+ system suggests that in utero it may mediate transfer of cationic amino acids from the syncytiotrophoblast to the fetus. The role of the high-affinity low-capacity bo,+ system remains to be determined.
Furesz
T C
TC
Department of Pediatrics, Washington University School of Medicine, St. Louis Children's Hospital, Missouri 63110.
Moe
A J
AJ
Smith
C H
CH
D000596:Amino Acids / Q000493:pharmacokinetics*; D001120:Arginine / Q000493:pharmacokinetics / Q000494:pharmacology; D001692:Biological Transport; D002412:Cations / Q000493:pharmacokinetics*; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D006801:Humans; D008239:Lysine / Q000037:antagonists & inhibitors / Q000493:pharmacokinetics; D009994:Osmolar Concentration; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D013997:Time Factors
D000596:Amino Acids; D002412:Cations; D001120:Arginine; D008239:Lysine