[Localization and modulation of galactosyltransferase during in vitro proliferation of a human ovarian adenocarcinoma cell line].
A cell line, IGROV1, originating from a human ovarian cancer, releases a galactosyltransferase activity in its culture medium during proliferation. The proliferating IGROV1 cells appear as two populations: some cells grow in floating clusters whereas the greater part of them adhere to the culture substrate. The study of galactose transfer by intact cells onto an exogenous glycoprotein acceptor (ovomucoid) shows the presence of surface-associated galactosyltransferase onto the two cellular sub-populations. Opposite to intracellular activity, surface-associated and released galactosyltransferase activities depend on cellular adhesion and proliferation.
Sichel
F
F
Centre régional de Lutte contre le Cancer F. Baclesse, Caen.
Bar
E
E
Gauduchon
P
P
Malas
J P
JP
Benard
J
J
Le Talaer
J Y
JY
D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology; D002448:Cell Adhesion; D002455:Cell Division; D002461:Cell Line, Transformed; D005260:Female; D005700:Galactosyltransferases / Q000378:metabolism* / Q000493:pharmacokinetics; D006801:Humans; D066298:In Vitro Techniques; D010051:Ovarian Neoplasms / Q000201:enzymology* / Q000473:pathology
D005700:Galactosyltransferases
Localisation et modulation de la galactosyltransférase au cours de la prolifération in vitro d'une lignée cellulaire issue d'un adénocarcinome ovarien humain.