The taming of immunohistochemistry: the new era of quality control.
The most critical factor for interpreting the results of immunohistochemistry is verification of antibody sensitivity and specificity. While some manufacturers supply material data sheets with this information, many do not. This paper describes a well-defined quality assurance program for testing immune reagents. This program can be used to provide commercial suppliers of antisera with analyses of their products destined for government licensure applications. This paper illustrates the protocol and explains the testing philosophy developed over the last eight years.
Herman
G E
GE
Sinai Hospital, Department of Laboratory Medicine, Detroit, Michigan 48235.
Elfont
E A
EA
D007150:Immunohistochemistry / Q000592:standards*; D007202:Indicators and Reagents / Q000592:standards; D011786:Quality Control; D012680:Sensitivity and Specificity
D007202:Indicators and Reagents