A method for sequencing polymerase chain reaction products can be used to sequence Bacillus subtilis "miniprep" plasmid DNA.
Several methods for sequencing double-stranded plasmid DNA isolated from E. coli have been described. These methods are usually not effective when used to sequence plasmid DNA isolated from Bacillus subtilis. In the course of developing a simplified version of a previously published protocol for polymerase chain reaction product sequencing, it was found that this protocol could be used for sequencing plasmid DNA isolated from Bacillus subtilis.
Bechhofer
D H
DH
Department of Biochemistry, Mount Sinai School of Medicine, New York, NY 10029.
D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*
D004269:DNA, Bacterial