title: Preparation of lyophilized heat-treated cryoprecipitates from a small pool of plasma obtained by apheresis.
abstract: A method was developed for the batch-wise production of small-pool lyophilized heat-treated cryoprecipitates in a regional Blood Bank. Ten vials of final product were derived from twenty 600 g apheresis plasma donations. We found 384 IU of factor VIII per vial and a specific activity of 0.20 IU per mg total protein. Production recovery was 340 IU of factor VIII per kg plasma. The method was easy to perform and gave a product of good quality.
authors.affiliation: Central Pharmacy of the Hospitals of The Hague, The Netherlands.
authors.forename: D P
authors.initials: DP
authors.lastname: Allersma
authors.forename: E
authors.initials: E
authors.lastname: Roeffen
authors.forename: J A
authors.initials: JA
authors.lastname: Van der Does
authors.forename: E
authors.initials: E
authors.lastname: Briët
mesh_terms: D001781:Blood Component Removal / Q000379:methods*; D005169:Factor VIII / Q000302:isolation & purification*; D005340:Fibrinogen / Q000302:isolation & purification*; D005612:Freeze Drying; D006358:Hot Temperature; D006801:Humans; D010956:Plasmapheresis; D011786:Quality Control
chemical_list: C026912:cryoprecipitate coagulum; D005169:Factor VIII; D005340:Fibrinogen