Interleukin-9 expression in human malignant lymphomas: unique association with Hodgkin's disease and large cell anaplastic lymphoma.
To test the possibility that interleukin-9 (IL-9), the human homologue of the mouse T-cell growth factor P40, may be involved in the pathogenesis of human lymphomas, we examined IL-9 expression in a variety of tumors both by Northern blot analysis and by in situ hybridization. Of 18 B-cell non-Hodgkin's lymphomas and 11 peripheral T-cell lymphomas, none expressed IL-9 message. By contrast, IL-9 message was found in two of six cases of large cell anaplastic lymphoma (LCAL) and in 6 of 13 cases of Hodgkin's disease (HD). In HD the strongest signals were observed in Hodgkin (H) and Sternberg-Reed (SR) cells, but IL-9 mRNA was also detected in small lymphocytic cells. A search for IL-9 message in a panel of 20 cell lines derived both from hematopoietic and nonhematopoietic tumors confirmed the unique association of IL-9 expression with HD and LCAL in as much as the only two cell lines with IL-9 message were derived from cases of HD and LCAL. These results suggest that IL-9 is not involved as an autocrine growth factor in the pathogenesis of most B- and T-cell lymphomas, but that it may play a role in HD and LCAL.
Merz
H
H
Department of Pathology, University of Würzburg, Germany.
Houssiau
F A
FA
Orscheschek
K
K
Renauld
J C
JC
Fliedner
A
A
Herin
M
M
Noel
H
H
Kadin
M
M
Mueller-Hermelink
H K
HK
Van Snick
J
J
D015152:Blotting, Northern; D006689:Hodgkin Disease / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D016906:Interleukin-9; D007378:Interleukins / Q000096:biosynthesis*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000378:metabolism* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism
D016906:Interleukin-9; D007378:Interleukins; D012333:RNA, Messenger; D012334:RNA, Neoplasm