Trypanosoma cruzi: properties of a clone isolated from CL strain.
BALB/c mice injected i.p. with 2 x 10(6) metacyclic forms of CL-14, a clone isolated from the CL strain of Trypanosoma cruzi, did not show parasitemia as evaluated by direct blood microscopy examination, hemoculture and xenodiagnosis. Moreover, new-born mice (1-2 days old) injected with culture- or insect-derived CL-14 trypomastigotes also displayed negative parasitemia. No mortality was observed in either group of animals. However, despite this apparent non-infectivity, mice injected with clone 14 developed high resistance against a lethal challenge with virulent trypomastigotes. All challenged mice survived and the parasitemia was negative. These results indicate that clone 14 is a very good antigen for the study of acquired immunity in T. cruzi infection and, therefore, a potential candidate for the development of a vaccine against this parasite.
Lima
M T
MT
Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Brazil.
Jansen
A M
AM
Rondinelli
E
E
Gattass
C R
CR
D000818:Animals; D000831:Animals, Newborn; D014355:Chagas Disease / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D007110:Immunity, Active; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D014349:Trypanosoma cruzi / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*