Immunological and HPLC detection of aflatoxin adducts in human tissues after an acute poisoning incident in S.E. Asia.
Acid hydrolysed, purified DNA, extracted from formalin fixed human tissues from persons acutely exposed to aflatoxins during a poisoning incident, was found to inhibit antibody binding in a competitive aflatoxin inhibition ELISA both before and after immunoaffinity column purification. HPLC analysis of acid hydrolysates of the DNA revealed a peak with a retention time 3 min earlier than 8,9-dihydro-2-(N7-guanyl)-9-hydroxy AFB1 synthesized either by peracid activation or direct reaction of the 8,9-oxide with DNA. The major peak seen when DNA was extracted from formalin fixed tissues from rats treated with aflatoxin B1 was identical to that seen in the formalin fixed human tissues. Adduct levels ranged from 0 to 170 x 10(6) nucleotides depending on tissue type and individual examined.
Harrison
J C
JC
Department of Biology, University of York, Heslington, UK.
Garner
R C
RC
D000293:Adolescent; D000328:Adult; D016604:Aflatoxin B1; D000348:Aflatoxins / Q000032:analysis*; D000368:Aged; D001210:Asia, Southeastern; D002273:Carcinogens / Q000032:analysis; D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives* / Q000032:analysis; D006801:Humans; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D011041:Poisoning / Q000378:metabolism*; D000096702:Aflatoxin Poisoning
D000348:Aflatoxins; D002273:Carcinogens; D006147:Guanine; D004247:DNA; D016604:Aflatoxin B1