Microheterogeneity of urinary albumin and tubular proteinuria in juvenile diabetes mellitus.
We studied differential urinary albumin excretion by a double one-dimensional gel electrophoresis with decyl sodium sulphate-polyacrylamide gel electrophoresis in the first, and isoelectric focusing in the second dimension in 37 diabetic children and 20 healthy subjects. In addition, total proteins, albumin, beta 2-microglobulin and molecular size distribution of urinary proteins were measured, the latter using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Whilst albuminuria was not significantly different from controls we found an increased microheterogeneity of urinary albumin in 38% of patients. In addition, low molecular weight protein (P less than 0.05) and beta 2-microglobulin excretion (P less than 0.01) were elevated. It is suggested that the appearance of highly heterogenous albumin in the pI range of 5.3-5.9 is the result of a decreased tubular reabsorption.
Ries
M
M
Division of Pediatric Nephrology, University Children's Hospital, Heidelberg, Federal Republic of Germany.
Schärer
K
K
Wartha
R
R
Schmidt
H
H
Gekle
D
D
D000293:Adolescent; D000328:Adult; D000419:Albuminuria / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000652:urine*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D008970:Molecular Weight; D011507:Proteinuria / Q000652:urine*; D001613:beta 2-Microglobulin / Q000652:urine
D001613:beta 2-Microglobulin