title: Decline in cholesteryl ester hydrolase activity of rat brain with progress of experimental allergic encephalomyelitis followed by rebound during recovery.
abstract: Cholesteryl ester hydrolase and cholesteryl esters were measured in brain homogenates from rats in various stages of experimental allergic encephalomyelitis (EAE), a model for multiple sclerosis, and in age-matched controls. Cholesteryl esters were elevated approximately 280-507% in EAE rats with clinical indices of 1-3, corresponding to increasing severity of symptoms, but were not significantly different from controls upon disappearance of symptoms during the recovery period (stage = R2). Cholesteryl ester hydrolase declined progressively 12-34% during stages 1-3, rebounded to a level 16% greater than controls during the initial stage of recovery (R1) and remained significantly elevated by 6% over controls upon disappearance of symptoms (R2). A highly significant (P less than 0.001) correlation of reduction in cholesteryl ester hydrolase activity with clinical index was confirmed by linear regression analysis (R = 0.91). It is concluded that a decline in cholesteryl ester hydrolase activity is an early event in EAE which leads to accumulation of cholesteryl esters and may play a role in induction and progression of this demyelinating disorder.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0614.
authors.forename: S
authors.initials: S
authors.lastname: Ghosh
authors.forename: W M
authors.initials: WM
authors.lastname: Grogan
mesh_terms: D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000201:enzymology* / Q000503:physiopathology; D005260:Female; D051381:Rats; D011917:Rats, Inbred Lew; D012075:Remission, Spontaneous; D002787:Sterol Esterase / Q000378:metabolism*
chemical_list: D002787:Sterol Esterase