The immunobiology of murine interleukin-1 alpha encoded by recombinant vaccinia virus.
Recombinant vaccinia viruses were constructed which encoded murine interleukin-1 alpha (IL-1 alpha) (VV-IL1). One virus also encoded the hemagglutinin (HA) gene of influenza virus (VV-HA-IL1). Mice were infected with these viruses and the effects of co-expressed IL-1 on various immune parameters were assessed. The growth of VV-IL1 in vivo was less than that of the control virus, and this was reflected in the reduced virus-induced cell-mediated immune responses. However, specific antibody responses generated after challenge with vaccinia or influenza viruses were significantly higher when VV-HA-IL1 was used to prime mice, compared to the control virus (VV-HA-TK). This study demonstrates that co-expressed cytokines may be useful for selective alteration of immune reactivity.
Ruby
J
J
Division of Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra.
Fordham
S
S
Kasprzak
A
A
Osvath
S
S
Ramshaw
I
I
D000818:Animals; D000917:Antibody Formation; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D005260:Female; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000235:genetics*; D007111:Immunity, Cellular; D007375:Interleukin-1 / Q000235:genetics / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D008819:Mice, Nude; D011994:Recombinant Proteins / Q000276:immunology; D014162:Transfection; D014616:Vaccinia virus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral; D007375:Interleukin-1; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins