Effects of TZI-41127, a novel selective 5-lipoxygenase inhibitor, on A23187-induced pleurisy in rats.
Intrapleural injection of the ionophore A23187 in rats induced leukotriene (LT) production, prostaglandin E2 production, plasma exudation and leukocyte accumulation in the pleural cavity. We evaluated the inhibitory effects of orally administered drugs on 5-lipoxygenase activity by their ability to reduce the content of both peptido-LTs (LTC4, LTD4 and LTE4) and LTB4 in this model. TZI-41127, a novel selective 5-lipoxygenase inhibitor, significantly reduced the peptido-LTs and LTB4 content with ED50 values of 4.2 and 6.1 mg/kg, respectively, whereas it only reduced the prostaglandin E2 content (cyclooxygenase activity) by 31.1% even after 100 mg/kg. Phenidone inhibited 5-lipoxygenase activity more selectively than cyclooxygenase activity. BW755C inhibited cyclooxygenase activity more selectively than 5-lipoxygenase activity. Indomethacin selectively inhibited cyclooxygenase activity. These results suggest that: (1) A23187-induced pleurisy is an convenient in vivo model for studying drug effects on 5-lipoxygenase and cyclooxygenase activities and (2) TZI-41127 is an orally active and comparatively selective inhibitor of 5-lipoxygenase activity.
Hagiwara
M
M
Pharmacological Research Department, Teikoku Hormone Mfg. Co., Ltd., Kawasaki, Japan.
Mikami
T
T
Iwamura
S
S
Miyazawa
K
K
Kobayashi
M
M
Miyasaka
K
K
D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine / Q000494:pharmacology; D000818:Animals; D000001:Calcimycin; D002851:Chromatography, High Pressure Liquid; D016861:Cyclooxygenase Inhibitors; D015232:Dinoprostone / Q000378:metabolism; D007211:Indoles / Q000627:therapeutic use*; D007962:Leukocytes / Q000187:drug effects; D015289:Leukotrienes / Q000378:metabolism; D016859:Lipoxygenase Inhibitors; D008297:Male; D010994:Pleura / Q000473:pathology; D010998:Pleurisy / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D016861:Cyclooxygenase Inhibitors; D007211:Indoles; D015289:Leukotrienes; D016859:Lipoxygenase Inhibitors; C063918:2-(4-hydroxy-3,5-dimethylphenyl)-5-methoxy-3-methylindole; D000001:Calcimycin; D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D015232:Dinoprostone