Bombesin stimulates a high affinity GTPase activity in membranes of Swiss 3T3 fibroblasts.
Peptides of the bombesin family are mitogenic for Swiss 3T3 fibroblasts and in these cells stimulate the turnover of polyphosphoinositides. Recent studies have suggested that G protein(s) may be involved in the signal transduction pathway triggered by bombesin. In this study we have found and characterized a high affinity GTPase activity stimulated by bombesin in membranes of Swiss 3T3 fibroblasts. Our results support the involvement of a G protein in the response of Swiss 3T3 cells to bombesin.
Morello
L
L
Dipartimento di Fisiologia e Biochimica Generali, Università di Milano, Italy.
Sturani
E
E
Cattaneo
M G
MG
Brambilla
R
R
Vicario
A
A
Alberghina
L
L
Zippel
R
R
D000818:Animals; D001839:Bombesin / Q000494:pharmacology*; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology*; D005347:Fibroblasts; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism; D019886:Gastrin-Releasing Peptide; D006160:Guanosine Triphosphate / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D051379:Mice; D010455:Peptides / Q000494:pharmacology; D010710:Phosphates / Q000032:analysis; D015398:Signal Transduction
D007295:Inositol Phosphates; D010455:Peptides; D010710:Phosphates; D019886:Gastrin-Releasing Peptide; D006160:Guanosine Triphosphate; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D008274:Magnesium; D001839:Bombesin