Demonstration of murine pancreas elastase and its interstrain variation by isoelectric focusing.
Isoelectric focusing between pH 9 and 11 was used for separation of murine pancreas proteases. One of these proteases is characterized by its preference for N-acetyl-L-alanine-alpha-naphthylester as substrate and by its genetic linkage to bt, a coat color marker of chromosome 15. This protease was identified as elastase, and is probably elastase-1 (ELA-1). Because of the simple procedure and the excellent reproducibility of the focusing pattern, ELA-1 is recommended as a useful marker for mouse chromosome 15.
von Deimling
O H
OH
Abteilung für Chemische Pathologie, Universität Freiburg, Germany.
Gaa
A
A
Hameister
H
H
D000818:Animals; D014644:Genetic Variation; D007525:Isoelectric Focusing; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB; D010179:Pancreas / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000235:genetics*; D013045:Species Specificity
D010196:Pancreatic Elastase