Structure of laminin variants. The 300-kDa chains of murine and bovine heart laminin are related to the human placenta merosin heavy chain and replace the a chain in some laminin variants.
A variant of laminin has previously been isolated from murine heart and shown to be distinct from laminin purified from a traditional source, the murine Engelbreth-Holm-Swarm (EHS) tumor (Paulsson, M., and Saladin, K. (1989) J. Biol. Chem. 264, 18726-18732). It contains a novel polypeptide chain designated as 300 kDa, which is not found in laminin from the EHS tumor. In the present study, heart laminin was purified from bovine tissue and shown to be structurally and immunochemically closely related to the murine protein. Further, heart laminins were compared with merosin, a laminin-like protein isolated from human placenta (Ehrig, K., Leivo, I., Argraves, W. S., Ruoslahti, E., and Engvall, E. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 3264-3268). The 300-kDa chain of bovine heart laminin cross-reacted with the heavy chain of merosin, showing that these polypeptides are closely related, albeit from different species. Heart laminin is more resistant to proteolysis than laminin derived from the EHS tumor. A large fragment could be prepared by digestion with thermolysin, which consisted of an almost intact long arm structure and variably long, residual short arm structures. Analysis of its structure shows that the 300-kDa heavy chain is disulfide-bonded to the B1 and B2 chains in the center of the laminin cross and forms the long arm together with these chains. It thereby replaces the A chain, well known from tumor sources, in the laminin structure.
Paulsson
M
M
M. E. Müller-Institute for Biomechanics, University of Bern, Switzerland.
Saladin
K
K
Engvall
E
E
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D007797:Laminin / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure; D008565:Membrane Proteins / Q000737:chemistry*; D051379:Mice; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009206:Myocardium / Q000737:chemistry*; D010196:Pancreatic Elastase; D010920:Placenta / Q000737:chemistry*; D011487:Protein Conformation; D013820:Thermolysin
D007797:Laminin; D008565:Membrane Proteins; D010196:Pancreatic Elastase; D013820:Thermolysin