title: Imipenem resistance in pseudomonas aeruginosa PAO: mapping of the OprD2 gene.
abstract: Carbapenem antibiotics have been shown to penetrate the outer membrane of Pseudomonas aeruginosa through a unique porin protein, OprD2. We mapped the OprD2 gene by conjugation using plasmid FP2 and by transduction using phage F116L. This gene maps between 71 and 75 min on the PAO1 chromosome.
authors.affiliation: Department of Medicine, Michael Reese Hospital, Chicago, Illinois 60616.
authors.forename: J P
authors.initials: JP
authors.lastname: Quinn
authors.forename: A
authors.initials: A
authors.lastname: Darzins
authors.forename: D
authors.initials: D
authors.lastname: Miyashiro
authors.forename: S
authors.initials: S
authors.lastname: Ripp
authors.forename: R V
authors.initials: RV
authors.lastname: Miller
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D001435:Bacteriophages; D002874:Chromosome Mapping; D003470:Culture Media; D004352:Drug Resistance, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D015378:Imipenem / Q000494:pharmacology*; D009154:Mutation; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics; D014161:Transduction, Genetic
chemical_list: D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D015378:Imipenem