Isotachophoretic determination of 2-5A phosphodiesterase.
Together with 2-5A synthetase and ribonuclease L, 2-5A phosphodiesterase belongs to the 2-5A system, which plays an important role in the action of interferon. Analytical capillary isotachophoresis was used for the determination of 2-5A phosphodiesterase activity. Enzyme assay was optimized using snake venom phosphodiesterase as a source of 2-5A phosphodiesterase activity. The 2-5A trimer core was used as a substrate. Enzyme activity was determined in time- and concentration-dependent reactions. In addition, 2-5A phosphodiesterase activity was determined in lysates of mononuclear blood cells.
Bruchelt
G
G
Children's Hospital, University of Tübingen, Germany.
Buedenbender
M
M
Schmidt
K H
KH
Jopski
B
B
Treuner
J
J
Niethammer
D
D
D000227:Adenine Nucleotides / Q000737:chemistry / Q000378:metabolism; D004586:Electrophoresis; D005095:Exoribonucleases / Q000378:metabolism*; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000201:enzymology; D015394:Molecular Structure; D009843:Oligoribonucleotides / Q000737:chemistry / Q000378:metabolism
D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; C023505:2',5'-oligoadenylate; D005095:Exoribonucleases; C029364:2'-phosphodiesterase