A serum-free medium for the culture of insect cells and production of recombinant proteins.
A low protein aqueous lipid supplement (Ex-Cyte VLE), in combination with pluronic polyol, is an effective replacement for fetal bovine serum for insect Sf-9 cells. Serum-free medium with lipid supplement and pluronic (SFM-LP) supported higher cell viability and maximum cell populations than serum-supplemented medium. No adaptation procedures are required when switching cells from serum-containing medium to SFM-LP, and growth rates remain constant during continued passages in SFM-LP. The amounts of recombinant proteins produced, which is the major use for the Sf-9 cells, are better or equal in SFM-LP compared to serum-supplemented medium. SFM-LP also supports growth of the TN-368 cell line but IPLB-SF-21AE or IZD-Mb0503 lines grow poorly in this medium.
Hink
W F
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Ohio State University, Department of Entomology, Columbus 43210.
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001769:Blood; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D006801:Humans; D007700:Kinetics; D008055:Lipids / Q000494:pharmacology; D008747:Methylcellulose / Q000494:pharmacology; D009036:Moths / Q000166:cytology* / Q000187:drug effects; D010958:Plasminogen / Q000235:genetics; D011060:Poloxalene / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis*; D001616:beta-Galactosidase / Q000235:genetics
D003470:Culture Media; D008055:Lipids; D011994:Recombinant Proteins; D010958:Plasminogen; D011060:Poloxalene; D008747:Methylcellulose; D001616:beta-Galactosidase