[The role of gamma-interferon in blister formation of bullous pemphigoid].
Recently it has been reported that cell-mediated immune (CMI) reaction is related to the blister formation as well as the reaction of autoantibody against the basement membrane zone (BMZ-Ab) in bullous pemphigoid (BP). Previously we reported that T-cells infiltrated were producing gamma-interferon (IFN-gamma) and that high levels of IFN-gamma were detected in the blister fluids of BP. In the present study, in order to find the effect of IFN-gamma on the skin, we have done the organ culture of normal skin explants with IFN-gamma. The dermal-epidermal separation (DES) was histologically observed in skin explants which were incubated with high level of IFN-gamma after 24 hours. The DES was found to be located between the basal layer and the site of laminin and type IV collagen. It is considered that IFN-gamma alters the antigenicity and mediates to release some other cytokines in CMI reaction. In addition to these, IFN-gamma seems to directly work on the DES in normal skin. Around the DES of the skin explants, plasminogen activator was accelerated immunohistologically. The results suggest that IFN-gamma mediated by CMI response also plays an important role as well as the autoantibody in the blister formation of BP.
Takiguchi
Y
Y
Department of Dermatology, Fukushima Medical College, Japan.
Itoh
N
N
Suzuki
M
M
Kaneko
F
F
Minagawa
T
T
D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000276:immunology; D001768:Blister / Q000276:immunology; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000494:pharmacology / Q000502:physiology*; D008875:Middle Aged; D009924:Organ Culture Techniques; D010391:Pemphigoid, Bullous / Q000276:immunology*; D011994:Recombinant Proteins
D001323:Autoantibodies; D011994:Recombinant Proteins; D007371:Interferon-gamma