A device for freeze-substituting a large number of samples under controlled conditions.
An inexpensive device for reproducible freeze-substitution is described. The equipment consists of a reciprocating shaker, holding vials of substitution fluid, mounted in the gas phase of a large liquid nitrogen refrigerator. The shaker temperature is controlled by a programmable microprocessor.
Marshall
A T
AT
Department of Zoology, La Trobe University, Bundoora, Victoria, Australia.
Kent
M
M
D000818:Animals; D005615:Freezing; D008099:Liver / Q000648:ultrastructure*; D009584:Nitrogen; D051381:Rats; D015203:Reproducibility of Results; D013048:Specimen Handling
D009584:Nitrogen