title: Three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina.
abstract: The three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina has been determined at a resolution of 2 nm in the plane and 4 nm in the vertical direction by electron microscopy and computerized image processing on microcrystals of the enzyme. The enzyme forms a large ring-shaped complex containing six each of the large (62-kDa) and small (26-kDa) subunits. The complex is very open, with six well-separated dumbbell-shaped masses surrounding a large cylindrical hole. Each dumbbell is interpreted as consisting of one large and one small subunit.
authors.affiliation: Institute of Crystallography, USSR Academy of Sciences, Moscow.
authors.forename: M B
authors.initials: MB
authors.lastname: Sherman
authors.forename: E V
authors.initials: EV
authors.lastname: Orlova
authors.forename: E A
authors.initials: EA
authors.lastname: Smirnova
authors.forename: S
authors.initials: S
authors.lastname: Hovmöller
authors.forename: N A
authors.initials: NA
authors.lastname: Zorin
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D006091:Gram-Negative Chemolithotrophic Bacteria / Q000201:enzymology*; D006864:Hydrogenase / Q000737:chemistry*; D008854:Microscopy, Electron; D008958:Models, Molecular; D008968:Molecular Conformation
chemical_list: D006864:Hydrogenase