title: Eukaryotic initiation factor 5A: the molecular form of the hypusine-containing protein from human erythrocytes.
abstract: Eukaryotic initiation factor 5A (eIF-5A, formerly known as eIF-4D) purified from human erythrocytes has been found to have a monomeric molecular weight between 17,500 and 18,000. In this study, using exclusion chromatography and analytical ultracentrifugation, we demonstrate that eIF-5A normally exists as a dimer in solution and appears to be capable of undergoing reversible association to form higher polymers.
authors.affiliation: National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892.
authors.forename: S I
authors.initials: SI
authors.lastname: Chung
authors.forename: M H
authors.initials: MH
authors.lastname: Park
authors.forename: J E
authors.initials: JE
authors.lastname: Folk
authors.forename: M S
authors.initials: MS
authors.lastname: Lewis
mesh_terms: D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000032:analysis; D008970:Molecular Weight; D010448:Peptide Initiation Factors / Q000097:blood* / Q000302:isolation & purification; D011489:Protein Denaturation; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A
chemical_list: D008239:Lysine; D010448:Peptide Initiation Factors; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A; C100028:hypusine