title: A simple handling technique for mammalian oocytes and embryos during preparation for transmission electron microscopy.
abstract: Due to their small size, mammalian oocytes and embryos pose unique problems during preparation for transmission electron microscopy. This paper outlines a method which combines protein embedding with centrifugation to locate the specimens on the face of a Beem capsule mould. This method facilitates both the processing of oocytes with minimal loss and rapid location of the specimens within the block for simultaneous sectioning, staining and examination.
authors.affiliation: Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, Canada.
authors.forename: A P
authors.initials: AP
authors.lastname: Britton
authors.forename: Y S
authors.initials: YS
authors.lastname: Moon
authors.forename: B H
authors.initials: BH
authors.lastname: Yuen
mesh_terms: D000818:Animals; D002498:Centrifugation; D004622:Embryo, Mammalian / Q000648:ultrastructure*; D005260:Female; D005976:Glutaral; D006652:Histological Techniques; D008854:Microscopy, Electron; D009865:Oocytes / Q000648:ultrastructure*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005976:Glutaral