Repeat sequences from complex ds DNA viruses can be used as minisatellite probes for DNA fingerprinting.
In a search for new fingerprinting probes for use with sheep, repeat sequences derived from five poxviruses, an iridovirus and a baculovirus were screened against DNA from sheep pedigrees. Probes constructed from portions of the parapox viruses, orf virus and papular stomatitis virus and the baculovirus from the alfalfa looper, Autographa californica, nuclear polyhedrosis virus all gave fingerprint patterns. Probes from three other poxviruses and an iridovirus did not give useful banding patterns.
Crawford
A M
AM
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Buchanan
F C
FC
Fraser
K M
KM
Robinson
A J
AJ
Hill
D F
DF
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods / Q000662:veterinary*; D015342:DNA Probes; D004267:DNA Viruses / Q000235:genetics*; D004279:DNA, Viral; D007497:Iridoviridae / Q000235:genetics; D008969:Molecular Sequence Data; D011212:Poxviridae / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012756:Sheep / Q000235:genetics*
D015342:DNA Probes; D004279:DNA, Viral