title: Cell surface expression of the short immunoglobulin mu chain (D mu protein) in murine pre-B cells is differently regulated from that of the intact mu chain.
abstract: Pre-B cells carrying DJH rearrangements in an appropriate reading frame produce a short mu protein consisting of a DJH and the C mu & sequence (D mu protein). We analyzed a D mu-producing Abelson-murine leukemia virus-transformed murine pre-B line, 300-19, and demonstrated that D mu proteins are expressed on the cell surface in association with surrogate L chain proteins (lambda 5 and VpreB). However, when we introduced an expression vector coding for the D mu protein into the null pre-B line P17-27, which produce lambda 5 and VpreB but no Ig molecules, most of the cells did not express D mu proteins on the cell surface although D mu proteins were produced intracellularly. On the other hand, P17-27 brings intact mu chains on the cell surface, when a vector coding for the intact mu chain is introduced. Thus, cell surface expression of the D mu protein has different requirements from that of the intact mu chain. A possible role of the VH protein encoded by germ-line VH transcripts is discussed.
authors.affiliation: Max-Planck-Institute für Immunobiologie, Freiburg, FRG.
authors.forename: T
authors.initials: T
authors.lastname: Tsubata
authors.forename: R
authors.initials: R
authors.lastname: Tsubata
authors.forename: M
authors.initials: M
authors.lastname: Reth
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D007148:Immunoglobulin mu-Chains / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D051379:Mice; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*
chemical_list: D007148:Immunoglobulin mu-Chains; D011947:Receptors, Antigen, B-Cell