Acid phosphatase response in murine rhabdomyosarcoma for various tumour volumes and after different doses of neutron irradiation, alone or combined with exogenous ATP.
Acid phosphatase activity measured in a methylocholanthrene-induced murine rhabdomyosarcoma showed a monotonically increasing relation between enzyme activity and tumour volume. This could be related to the lytic activity of the enzyme in large tumours which become more hypoxic and necrotic, and hence enhance degradation and turnover of damaged tumour cells. The tumours were also subjected to irradiation using doses of 2.0, 3.8 and 6.0 Gy from a neutron therapy facility p(66MeV)/Be. The correlation between different doses and response of acid phosphatase activity could reflect the relation of magnitude of damage from metabolic disturbances, with dose. Furthermore exogenous ATP was shown to provide radioprotective action against neutron irradiation in two different experiments. The ATP reduced the activity of this lytic enzyme in irradiated tumours and also decreased tumour growth delay. This radioprotective role of exogenous ATP in a murine tumour could be related to physiological regulatory processes during defence mechanisms to maintain self-organisation in response to the radiation damage.
Szeinfeld
D
D
Research Institute for Medical Biophysics, S A Medical Research Council, Tygerberg, South Africa.
de Villiers
N
N
D000135:Acid Phosphatase / Q000032:analysis / Q000187:drug effects / Q000528:radiation effects*; D000255:Adenosine Triphosphate / Q000627:therapeutic use*; D000818:Animals; D003131:Combined Modality Therapy; D004354:Drug Screening Assays, Antitumor; D004735:Energy Transfer; D005214:Fast Neutrons; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011879:Radiotherapy Dosage; D012208:Rhabdomyosarcoma / Q000188:drug therapy / Q000201:enzymology / Q000532:radiotherapy*; D013997:Time Factors
D000255:Adenosine Triphosphate; D000135:Acid Phosphatase