Influence of major histocompatibility complex haplotype on the mitogenic response of T cells to staphylococcal enterotoxin B.
The abilities of antigen-presenting cells (APC) from nine independent major histocompatibility complex haplotypes and a number of intra-H-2 recombinant congenic strains of mice to present staphylococcal enterotoxin B (SEB) and induce proliferation in murine T-cell receptor V beta 8+ T-cell clones were compared. SEB presented by APC of all haplotypes tested induced significant responses in each of the T-cell clones. The magnitude of response was similar for most haplotypes, but there were limited quantitative differences between certain haplotypes. SEB presented by APC from H-2b mice as well as the intra-H-2 recombinant strains B10.GD and B10.A(4R), which do not express cell surface I-E (designated I-E-), induced the poorest T-cell responses. However, APC from AfE-, AsE-, and AqE- mice were as potent in SEB presentation as APC expressing both I-A and I-E. Antibodies against I-E were more effective than anti-I-A antibodies at inhibiting responses to SEB presented by APC expressing both I-A and I-E, whereas responses induced by APC expressing I-A but not I-E were blocked by antibodies against I-A. Thus, our results show that I-A can present SEB efficiently but that expression of both I-A and I-E on the same APC results in presentation of SEB predominantly by I-E. In addition, experiments using four distinct I-E- strains of mice indicate that I-A alleles differ in their ability to present SEB.
Robinson
J H
JH
Department of Immunology, Medical School, University of Newcastle upon Tyne, United Kingdom.
Pyle
G
G
Kehoe
M A
MA
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D002874:Chromosome Mapping; D004768:Enterotoxins / Q000633:toxicity*; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000235:genetics*; D006239:Haplotypes; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D013211:Staphylococcus aureus / Q000472:pathogenicity*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D004768:Enterotoxins; D006183:H-2 Antigens; C031818:enterotoxin B, staphylococcal