Control of components of bacterial polysaccharide vaccines by physical methods.
Analysis of polysaccharide components of meningococcal- and pneumococcal vaccines was carried out by proton n.m.r. spectroscopy and gas chromatography. The meningococcal polysaccharides were of high purity but showed differences in degree and position of O-acetylation between manufacturers. The level of contamination of the pneumococcal polysaccharides by C-substance was quantified. These methods provide an alternative to immunological methods for determining serotype and purity.
Jones
C
C
Chemistry Division, National Institute for Biological Standards and Control, South Mimms, Herts, U.K.
Currie
F
F
D001428:Bacterial Vaccines / Q000032:analysis*; D002240:Carbohydrate Sequence; D005410:Flame Ionization; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000032:analysis / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000032:analysis / Q000276:immunology
D001428:Bacterial Vaccines; D011135:Polysaccharides, Bacterial