Stoichiometric interaction of smg p21 with its GDP/GTP exchange protein and its novel action to regulate the translocation of smg p21 between membrane and cytoplasm.
We have previously purified a GDP/GTP exchange protein for smg p21A and -B, members of a ras p21/ras p21-like small GTP-binding protein superfamily. This regulatory protein, named smg p21 GDP dissociation stimulator (GDS), stimulates the dissociation of both GDP and GTP from and the subsequent binding of both GDP and GTP to smg p21s. We show here that smg p21 GDS forms a complex with both the GDP- and GTP-bound forms of smg p21B at a molar ratio of about 1:1. Both the GDP- and GTP-bound forms of smg p21B bound to membranes. smg p21 GDS inhibited this binding and moreover induced the dissociation of the prebound smg p21B from the membranes. These results indicate that smg p21 GDS stoichiometrically interacts with smg p21B and thereby regulates its GDP/GTP exchange reaction and its translocation between membranes and cytoplasm.
Kawamura
S
S
Department of Biochemistry, Kobe University School of Medicine, Japan.
Kaibuchi
K
K
Hiroyoshi
M
M
Hata
Y
Y
Takai
Y
Y
D000818:Animals; D001667:Binding, Competitive; D001792:Blood Platelets / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D002417:Cattle; D019204:GTP-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances; D020559:Monomeric GTP-Binding Proteins; D011485:Protein Binding; D051381:Rats; D013570:Synaptic Membranes / Q000378:metabolism; D020668:rap GTP-Binding Proteins
D046911:Macromolecular Substances; C062744:RAB3B protein, Bos taurus; D019204:GTP-Binding Proteins; D020559:Monomeric GTP-Binding Proteins; D020668:rap GTP-Binding Proteins