Detection and identification of loline and its analogues in horse urine.
Several kinds of loline-type alkaloids, norloline, loline, N-acetylnorloline, N-acetylloline, N-formylnorloline, N-formylloline and N-methylloline were detected in the urine of race-horses. Furthermore, a new compound of the alkaloids, N-senecioylnorloline, was also found and identified. These compounds were mainly identified by means of gas chromatography-mass spectrometry (GC-MS) and gas chromatography-fourier transform-infrared spectrometry (GC-FT-IR). A certain plant of Gramineae containing four kinds of loline-type alkaloids was found in a bale of hay used for the horse forage. The taxonomic feature of the plant was different from known plants containing loline-type alkaloids. The common fragmentation of loline-type alkaloids under electron ionization was briefly discussed.
Takeda
A
A
Laboratory of Racing Chemistry, Tokyo, Japan.
Suzuki
E
E
Kamei
K
K
Nakata
H
H
D000470:Alkaloids / Q000652:urine*; D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D006736:Horses / Q000652:urine*; D010944:Plants / Q000032:analysis; D013055:Spectrophotometry, Infrared
D000470:Alkaloids; C059478:loline