title: Lectin reactivity of murine fibrosarcoma lines with a different metastatic potential.
abstract: Lectins are suitable tools for investigating the glycoconjugate characteristics of metastatic cells. In the present study, we investigated whether there were differences between high metastatic T3 cells and a low metastatic isolate in their reactivities to several lectins specific for galactosyl and sialyl groups. Analysis of reactivity of the two cell lines to wheat germ agglutinin revealed a complex pattern. In fact, T3 cells had high-affinity, neuraminidase-resistant as well as low-affinity, neuraminidase-sensitive receptors. Instead, the low metastatic isolate showed only high-affinity receptors, both neuraminidase-resistant and neuraminidase-sensitive The two cell lines reacted similarly to galactose-specific lectins. These findings indicate that sialyl groups, rather than galactosyl groups, affect the metastatic behavior of our cell system.
authors.affiliation: Institute of General Pathology, University of Florence, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Mannori
authors.forename: G
authors.initials: G
authors.lastname: Mugnai
authors.forename: S
authors.initials: S
authors.lastname: Ruggieri
mesh_terms: D000818:Animals; D005354:Fibrosarcoma / Q000378:metabolism / Q000556:secondary*; D007457:Iodine Radioisotopes; D037102:Lectins / Q000378:metabolism*; D051379:Mice; D009439:Neuraminidase; D011975:Receptors, Mitogen / Q000032:analysis; D014407:Tumor Cells, Cultured
chemical_list: D007457:Iodine Radioisotopes; D037102:Lectins; D011975:Receptors, Mitogen; D009439:Neuraminidase