Characterization of a heparan sulfate and a peculiar chondroitin 4-sulfate proteoglycan from platelets. Inhibition of the aggregation process by platelet chondroitin sulfate proteoglycan.
A high molecular weight chondroitin sulfate proteoglycan (Mr 240,000) is released from platelet surface during aggregation induced by several pharmacological agents. Some details on the structure of this compound are reported. beta-Elimination with alkali and borohydride produces chondroitin sulfate chains with a molecular weight of 40,000. The combined results indicate a proteoglycan molecule containing 5-6 chondroitin sulfate chains and a protein core rich in serine and glycine residues. Degradation with chondroitinase AC shows that a 4-sulfated disaccharide is the only disaccharide released from this chondroitin sulfate, characterizing it as a chondroitin 4-sulfate homopolymer. It is shown that this proteoglycan inhibits the aggregation of platelets induced by ADP. Analysis of the sulfated glycosaminoglycans not released during aggregation revealed the presence of a heparan sulfate in the platelets. Degradation by heparitinases I and II yielded the four disaccharide units of heparan sulfates: N,O-disulfated disaccharide, N-sulfated disaccharide, N-acetylated 6-sulfated disaccharide, and N-acetylated disaccharide. The possible role of the sulfated glycosaminoglycans on cell-cell interaction is discussed in view of the present findings.
Nader
H B
HB
Departmento de Bioquímica, Escola Paulista de Medicina, São Paulo, Brazil.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001792:Blood Platelets / Q000737:chemistry* / Q000187:drug effects; D002809:Chondroitin Sulfates / Q000378:metabolism*; D002810:Chondroitinases and Chondroitin Lyases / Q000737:chemistry; D002850:Chromatography, Gel; D004187:Disaccharides / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D006025:Glycosaminoglycans / Q000302:isolation & purification; D006497:Heparan Sulfate / Q000737:chemistry*; D008322:Mammals; D010975:Platelet Aggregation Inhibitors; D013045:Species Specificity
D000244:Adenosine Diphosphate; D000596:Amino Acids; D002809:Chondroitin Sulfates; D002810:Chondroitinases and Chondroitin Lyases; D004187:Disaccharides; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D010975:Platelet Aggregation Inhibitors