Development of an enzyme-linked immunosorbent assay of apolipoprotein E-AII complex in plasma.
Measurement of apolipoprotein (apo) E-AII complex in human plasma is important in determining the role of apoE in lipoprotein metabolism. In this paper, we demonstrate a new and simple method to determine apoE-All complex by using an enzyme-linked immunosorbent assay. Anti-apoE IgG (goat) was used as a capture antibody, and captured apoE-All complexes were detected by an anti-apoAll (rabbit) horseradish peroxidase-conjugated anti-rabbit IgG (goat) system. With this method, apoE-All complex was specifically determined without the interference of apoAll and was not affected by apoE monomer less than 250 mg/L. The content of the complex in reference serum, a normolipidemic serum pooled from five subjects with phenotype E3/E3, was arbitrarily defined as 100%. The coefficients of variation were 3.5%-6.3% within assay and 8.8%-11.6% between assays.
Tozuka
M
M
Central Clinical Laboratories, Shinshu University Hospital, Matsumoto, Japan.
Yoshida
Y
Y
Tanigami
J
J
Miyachi
M
M
Katsuyama
T
T
Kanai
M
M
D001057:Apolipoproteins E / Q000097:blood*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015203:Reproducibility of Results
D001057:Apolipoproteins E