Significance of variation in turnover of glycated albumin on indices of diabetic control.
We measured the half-time of disappearance of 125I-labelled glycated albumins in a rat model of diabetes with continuous infusion of physiological saline and insulin. Our results indicate that (i) in non-diabetic rats, continuous infusion of saline per se did not affect the concentrations of glucose or of fructosamine, and the half-time of disappearance of albumin was unaffected by degree of glycation; (ii) hyperglycaemia (mean plasma glucose concentration of 18-27 mmol/l) caused a small but significant increase in half-time of labelled glycated albumin disappearance from a mean of 42 h to a mean of 47 h; (iii) this effect of hyperglycaemia outweighed any effect of increase in albumin excretion detected in poorly controlled diabetic rats without infusion. We conclude that the effect of hyperglycaemia in slowing turnover of glycated albumin is likely to be insignificant in relation to its effect in promoting glycation, and may be species-dependent. However, in nondiabetics, variation of turnover of glycated albumin may well be significant in explaining the wide interindividual variation in concentration of glycated protein.
Johnson
R N
RN
Department of Clinical Biochemistry, Green Lane Hospital, Auckland, New Zealand.
Easdale
R W
RW
Tatnell
M
M
Baker
J R
JR
D000419:Albuminuria / Q000652:urine; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D002850:Chromatography, Gel; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000652:urine; D005260:Female; D019270:Fructosamine; D017127:Glycation End Products, Advanced; D006029:Glycosuria / Q000652:urine; D006031:Glycosylation; D006595:Hexosamines / Q000097:blood / Q000652:urine; D006943:Hyperglycemia / Q000097:blood* / Q000652:urine; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000378:metabolism*; D013045:Species Specificity; D000093423:Glycated Serum Albumin
D001786:Blood Glucose; D017127:Glycation End Products, Advanced; D006595:Hexosamines; D012709:Serum Albumin; D019270:Fructosamine; D000093423:Glycated Serum Albumin