Diastereoselective hydrolysis of peptide esters by alkaline protease. Preparation of racemization-free peptides.
A new and practical enzymatic procedure for preparative diastereoselective hydrolysis of peptide esters using the alkaline protease alcalase as a catalyst was developed. This procedure has been successfully applied to the resolution of peptide diastereomers and synthesis of racemization free peptides.
Chen
S T
ST
Graduate Institute of Biochemical Sciences, National Taiwan University.
Wu
S H
SH
Wang
K T
KT
D000595:Amino Acid Sequence; D002384:Catalysis; D004952:Esters / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity; D013381:Subtilisins / Q000378:metabolism*
D004952:Esters; D010455:Peptides; D013381:Subtilisins