title: Crystallization and preliminary X-ray studies of a Bacillus subtilis and Thermus thermophilus HB8 chimeric 3-isopropylmalate dehydrogenase.
abstract: A chimeric gene was constructed by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, and expressed in Escherichia coli. The chimeric enzyme was crystallized in a size suitable for X-ray structure analysis. The crystal has a space group of P3(1)21 or P3(2)21, a = b = 77.1 A and c = 158.3 A, which is isomorphous with that of the native enzyme from T. thermophilus.
authors.affiliation: Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Kanagawa.
authors.forename: K
authors.initials: K
authors.lastname: Onodera
authors.forename: H
authors.initials: H
authors.lastname: Moriyama
authors.forename: A
authors.initials: A
authors.lastname: Takenaka
authors.forename: N
authors.initials: N
authors.lastname: Tanaka
authors.forename: N
authors.initials: N
authors.lastname: Akutsu
authors.forename: M
authors.initials: M
authors.lastname: Muro
authors.forename: T
authors.initials: T
authors.lastname: Oshima
authors.forename: K
authors.initials: K
authors.lastname: Imada
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Katsube
mesh_terms: D050539:3-Isopropylmalate Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics; D003001:Cloning, Molecular; D003460:Crystallization; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013824:Thermus / Q000201:enzymology / Q000235:genetics; D014961:X-Ray Diffraction
chemical_list: D011994:Recombinant Proteins; D000429:Alcohol Oxidoreductases; D050539:3-Isopropylmalate Dehydrogenase