title: Solution structure of human growth hormone-releasing factor fragment (1-29) by CD: characteristic conformational change on phospholipid membrane.
abstract: The conformations of synthetic human growth hormone-releasing factor fragment (1-29) in the presence and the absence of 1,2-dimyristoyl-sn-glycero-3-phosphorylglycerol liposome as well as in aqueous 2,2,2-trifluoroethanol solution were investigated by CD spectroscopy. The secondary structure of the peptide in each solution was analyzed by two methods. Both results show that the peptide has an unordered structure in the aqueous solution, whereas it folds into helical structure in the aqueous alcohol and in the phospholipid solution. In addition, although the peptide exists as almost complete helix in the 50 vol% aqueous alcohol (80-90% helicity), it does not reach full helicity even in the solution containing excess amount of phospholipid liposome (maximum 65-70% helicity). The conformational difference is explained by the characteristic amphipathy of the peptide, i.e., the necessity to twist the separated amphipathic helical parts in the interaction with the phospholipid membrane probably makes the helicity of the peptide decrease.
authors.affiliation: Research Institute for Polymers and Textiles, Ibaraki, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Honda
authors.forename: S
authors.initials: S
authors.lastname: Ohashi
authors.forename: H
authors.initials: H
authors.lastname: Morii
authors.forename: H
authors.initials: H
authors.lastname: Uedaira
mesh_terms: D000595:Amino Acid Sequence; D002942:Circular Dichroism; D013007:Growth Hormone-Releasing Hormone / Q000737:chemistry*; D006801:Humans; D008563:Membrane Lipids / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry*; D010743:Phospholipids / Q000378:metabolism / Q000494:pharmacology; D011487:Protein Conformation; D012996:Solutions
chemical_list: D008563:Membrane Lipids; D010446:Peptide Fragments; D010743:Phospholipids; D012996:Solutions; D013007:Growth Hormone-Releasing Hormone