title: Structural differences in solution and crystalline forms of met-myoglobin.
abstract: For several decades X-ray diffraction studies have been the paragon of biological structure studies at atomic resolution. Diffraction provides three-dimensional structure information, which is essential to our fundamental understanding of protein function. However, since X-ray diffraction cannot be done to atomic resolution on proteins in their native solution or membrane-bound state, the possibility exists that the conformations of the protein in crystals are slightly different from the conformations in solution, and attempts to interpret details of the structure may be misleading and without physiological relevance. In this paper, we show that this concern is justified for a familiar protein, myoglobin. Performing X-ray absorption fine structure experiments on both solution and crystalline met-myoglobin (met-Mb), we find significant differences in the local environment of the iron between the two states. Specifically, the average iron-nearest neighbor atom distance in the crystalline form is 0.05 A shorter than that in the solution form, and the iron-nearest neighbor bond is more rigid in the crystalline met-Mb. Possible artifactual explanations for the differences have been ruled out.
authors.affiliation: Institute for Structural and Functional Studies, Philadelphia, Pennsylvania 19104.
authors.forename: K
authors.initials: K
authors.lastname: Zhang
authors.forename: B
authors.initials: B
authors.lastname: Chance
authors.forename: K S
authors.initials: KS
authors.lastname: Reddy
authors.forename: I
authors.initials: I
authors.lastname: Ayene
authors.forename: E A
authors.initials: EA
authors.lastname: Stern
authors.forename: G
authors.initials: G
authors.lastname: Bunker
mesh_terms: D000818:Animals; D003460:Crystallization; D008297:Male; D008786:Metmyoglobin / Q000737:chemistry*; D012996:Solutions; D013057:Spectrum Analysis; D013094:Spermatozoa / Q000737:chemistry; D014907:Whales; D014965:X-Rays
chemical_list: D012996:Solutions; D008786:Metmyoglobin