A new technique facilitating studies of scant cell specimens.
A simple method is described by which multiple cytological and cytochemical studies can be done on a clinical sample that contains relatively few cells. The cells are concentrated by centrifugation. The cell pellet is fixed, frozen and embedded in plastic. Thin (2-microns) sections are cut from the plastic. Thus, each cell may appear in several sections and many slides can be made from a single specimen. The advantages of this method over cytospins and Millipore filter preparations of cell suspensions are optimal utilization of all cells, excellent morphological and immunological preservation and ease and reproducibility of this technique.
Duarte
L
L
Department of Pathology, University of California, San Francisco 94143.
D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D008867:Microtomy; D007507:Therapeutic Irrigation; D014461:Ultracentrifugation; D001743:Urinary Bladder; D014556:Urine / Q000166:cytology*