Lysosomal and plasma membrane ganglioside GM3 sialidases of cultured human fibroblasts. Differentiation by detergents and inhibitors.
Cultured human fibroblasts contain two sialidases that degrade gangliosides such as GM3: a lysosomal activity that appears identical with the activity towards water-soluble substrates and that is deficient in the genetic lysosomal disorder sialidosis, and another enzyme that seems localized on the external surface of the plasma membrane. In this report we show that both enzymes can be differentiated in the presence of each other by choice of the detergent used for activation, and also by the inhibitory action of some polyanionic compounds such as sulphated glycosaminoglycans. The lysosomal ganglioside GM3 sialidase is greatly stimulated by sodium glycodeoxycholate and, to lesser degrees, by sodium glycocholate and sodium cholate. The ganglioside GM3 sialidase of the plasma membrane is not measurably active under the conditions of the lysosomal enzyme but is specifically activated by the non-ionic detergent Triton X-100. The glycodeoxycholate-stimulated, but not the Triton-activated, ganglioside GM3 sialidase activity was profoundly diminished in cell lines from patients with the lysosomal disorders sialidosis and galactosialidosis; however, both activities were normal in fibroblasts from patients with mucolipidosis IV, previously thought to be a ganglioside sialidase deficiency disorder. Both the lysosomal and the plasma membrane ganglioside GM3 sialidases were inhibited by sialic acids, suramin, dextran sulphate and sulphated glycosaminoglycans. Among the latter, heparin and heparan sulphate showed a much higher inhibitory potency towards the plasma membrane ganglioside GM3 sialidase than towards the lysosomal onw.(ABSTRACT TRUNCATED AT 250 WORDS)
Schneider-Jakob
H R
HR
Institut für Pathochemie und Allgemeine Neurochemie, Universität Heidelberg.
Cantz
M
M
D002239:Carbohydrate Metabolism, Inborn Errors / Q000201:enzymology*; D002462:Cell Membrane / Q000201:enzymology*; D002478:Cells, Cultured; D003902:Detergents / Q000494:pharmacology; D005347:Fibroblasts / Q000201:enzymology; D006002:Glycodeoxycholic Acid / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007700:Kinetics; D008247:Lysosomes / Q000201:enzymology*; D009083:Mucopolysaccharidoses / Q000201:enzymology*; D009439:Neuraminidase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012016:Reference Values; D012867:Skin / Q000201:enzymology
D003902:Detergents; D007527:Isoenzymes; D006002:Glycodeoxycholic Acid; D006493:Heparin; D009439:Neuraminidase