Contribution to the mode of action of univalent gold.
Au+ binds to the collagen structure and, therefore, the authors studied the kinetics of the interaction between sodium gold thiosulphate (SGTS) and C1q complement subcomponent, the structure of is partially collagenous. The kinetics was evaluated densitometrically and compared with that of collagen and SGTS. On the basis of those results, the effect of SGTS upon collagen type-II-induced arthritis (CIA) was investigated in Wistar rats. SGTS (20 mg per kg of body weight weekly) was administered i.m. starting i) with the first immunization dose, ii) with the second immunization dose, and iii) after the onset of arthritis. Regardless of the timing of drug administration the manifestation of arthritis was decreased, but the decrease was more expressed in the first two groups. It was concluded that SGTS administration is capable of inhibiting CIA, provided that the drug is applied sufficiently early. Since formation of antibodies to type-II collagen remained unaffected, it is feasible that the mode of action of gold complexes is based mainly on blocking the activation of complement system.
Novotná
J
J
Research Institute of Rheumatic Diseases, Czechoslovak Academy of Sciences, Prague, Czechoslovakia.
Miterová
L
L
Krabcová
M
M
Deyl
Z
Z
Adam
M
M
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D001169:Arthritis, Experimental / Q000276:immunology* / Q000473:pathology; D011232:Chemical Precipitation; D003094:Collagen / Q000276:immunology*; D015922:Complement C1q / Q000378:metabolism; D005260:Female; D012971:Gold Sodium Thiosulfate / Q000493:pharmacokinetics / Q000494:pharmacology*; D007596:Joints / Q000276:immunology* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains
D015922:Complement C1q; D003094:Collagen; D012971:Gold Sodium Thiosulfate