Autonomous DNA replication in human cells is affected by the size and the source of the DNA.
We previously developed short-term and long-term assays for autonomous replication of DNA in human cells. This study addresses the requirements for replication in these assays. Sixty-two random human genomic fragments ranging in size from 1 to 21 kb were cloned in a prokaryotic vector and tested for their replication ability in the short-term assay. We found a positive correlation between replication strength and fragment length, indicating that large size is favored for efficient autonomous replication in human cells. All large fragments replicated efficiently, suggesting that signals which can direct the initiation of DNA replication in human cells are either very abundant or have a low degree of sequence specificity. Similar results were obtained in the long-term assay. We also used the same assays to test in human cells a random series of fragments derived from Escherichia coli chromosomal DNA. The bacterial fragments supported replication less efficiently than the human fragments in the short-term and long-term assays. This result suggests that while the sequence signals involved in replication in human cells are found frequently in human DNA, they are uncommon in bacterial DNA.
Heinzel
S S
SS
Department of Genetics, Stanford University School of Medicine, California 94305.
Krysan
P J
PJ
Tran
C T
CT
Calos
M P
MP
D000818:Animals; D002460:Cell Line; D002934:Cinnamates; D004247:DNA / Q000096:biosynthesis / Q000737:chemistry*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000737:chemistry; D000882:Haplorhini; D006801:Humans; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D013045:Species Specificity
D002934:Cinnamates; D004269:DNA, Bacterial; D006921:Hygromycin B; C026273:hygromycin A; D004247:DNA