Determination of the branch location of extra N-acetyllactosamine units in sialo N-linked tetraantennary oligosaccharides.
An approach is presented for the determination of the branch location of 1 or 2 extra N-acetyllactosamine units in sialo N-linked carbohydrate chains from glycoproteins. Tetraantennary oligosaccharides containing extra N-acetyllactosamine units were digested with endo-beta-galactosidase, followed by treatment with N-acetyl-beta-glucosaminidase, yielding products which could be analysed by 1H-NMR spectroscopy, thereby giving conclusive data about the location of the extra units in the intact structures.
Hokke
C H
CH
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
Kamerling
J P
JP
van Dedem
G W
GW
Vliegenthart
J F
JF
D000118:Acetylglucosaminidase / Q000378:metabolism; D000606:Amino Sugars / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002240:Carbohydrate Sequence; D002460:Cell Line; D006224:Cricetinae; D004921:Erythropoietin / Q000737:chemistry; D006026:Glycoside Hydrolases; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D000606:Amino Sugars; D009844:Oligosaccharides; C016735:sialooligosaccharides; D004921:Erythropoietin; C000458:N-acetyllactosamine; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase; D000118:Acetylglucosaminidase