A new procedure for gangliosidic N-acetylneuraminic acid analysis in serum.
In this paper a method is presented which is suitable for the extraction, purification and analysis of serum gangliosides. The advantage in comparison with other previously published procedures is the complete extraction of sialoglycolipids without contamination of sialoglycoproteins and/or sialoglycopeptides. The method could be used as a second-level test for the diagnosis and follow-up of cancer patients, and also could be potentially used for pharmaco-kinetic studies after ganglioside treatment.
Gornati
R
R
Institute of General Physiology and Biological Chemistry, School of Pharmacy, University of Milano, Italy.
Rapelli
S
S
Montorfano
G
G
Cattaneo
C
C
Berra
B
B
D002725:Chloroform; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D005663:Furans; D005732:Gangliosides / Q000097:blood* / Q000302:isolation & purification*; D006801:Humans; D000432:Methanol; D019158:N-Acetylneuraminic Acid; D011402:Pronase; D012794:Sialic Acids / Q000097:blood* / Q000302:isolation & purification*
D005663:Furans; D005732:Gangliosides; D012794:Sialic Acids; C018674:tetrahydrofuran; D002725:Chloroform; D011402:Pronase; D019158:N-Acetylneuraminic Acid; D000432:Methanol