Influence of various adjuvants on the synthesis of specific antibodies of chicken, sheep and rabbit following immunization with an hapten.
In order to obtain high amounts of specific antibodies against the hapten methylphosphoric acid, para-aminophenyl-1,2,2-trimethyl-propyldiester (MATP) different animals were immunized with MATP coupled to the carrier protein human serum albumin (MATP12-HSA) using several adjuvants. The best specific immune response in sheep, rabbit and chicken was reached with Freund's complete adjuvant with animal specific differences being tested by an ELISA. The adjuvants aluminium hydroxide (5% and 10%) and diphosphoryl lipid A showed no significant difference compared to the control group (NaCl with MATP12-HSA). In rabbits and chickens MATP12-HSA can be used to reach an immune response without the help of an adjuvant.
Erhard
M
M
Institut für Physiologie, Physiologische Chemie und Ernährungsphysiologie, Tierärztliche Fakultät, Universität München, Germany.
Kellner
J
J
Kühlmann
R
R
Lösch
U
U
D000276:Adjuvants, Immunologic; D000818:Animals; D002645:Chickens / Q000276:immunology*; D005260:Female; D006241:Haptens / Q000276:immunology; D009943:Organophosphorus Compounds / Q000276:immunology*; D011817:Rabbits / Q000276:immunology*; D012756:Sheep / Q000276:immunology*
D000276:Adjuvants, Immunologic; D006241:Haptens; D009943:Organophosphorus Compounds; C056499:4-aminophenylphosphonic acid methyl-1,2,2-trimethylpropyl diester