Evidence for idiotypic sharing between conventional and naturally activated B cells.
In the primary immune response to hen egg-white lysozyme (HEL), approximately 50% of the primary anti-HEL antibody-forming cells (AFC) express IdXE, an idiotype absent from the secondary response. Spontaneous IgM enzyme-linked immunosorbent assay (ELISA)-AFC in spleen cells from naive A/J mice were analyzed for the occurrence of IdXE by use of two affinity-purified rabbit antisera, R213 and R8, each raised against a different primary IdXE+, anti-HEL mAb. Two ELISA-AFC assay methods were used: direct coating of immunoplates with R213 or development of IgM-producing ELISA-AFC with biotinylated R8. From ages 7 days until 6 months, 10-20% of spontaneous IgM AFC were found to be IdXE+. IdXE+ anti-HEL IgG1 monoclonal antibodies, 2F4 or 3C11 (100 micrograms/ml), completely inhibited binding of biotinylated R8 (0.5 micrograms/ml) to spontaneous IgM-AFC while IdXE-, anti-HEL IgG1, 5E11 and 2C7, showed no significant inhibition. Greater than 90% of IdXE+ spontaneous IgM-AFC were not HEL specific. We conclude that a dominant set of idiotopes found in a conventional antigen-driven immune response can also play a major role in the spontaneously activated B cell repertoire. Our data argue against a bifurcation of the immune system into a compartment of idiotypic network-related cells and an independent set of non-network cells subject to antigenic stimulation.
Song
C H
CH
Department of Pediatrics, UCLA School of Medicine, Torrance 90509.
Calandra
G B
GB
Miller
A
A
Sercarz
E E
EE
Keller
M A
MA
D000375:Aging / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000917:Antibody Formation; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000096:biosynthesis; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A / Q000276:immunology; D009113:Muramidase / Q000276:immunology; D012217:Rheumatoid Factor / Q000096:biosynthesis / Q000276:immunology
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M; D012217:Rheumatoid Factor; D009113:Muramidase