Killing of intracellular Mycobacterium tuberculosis by receptor-mediated drug delivery.
p-Aminosalicylic acid (PAS) conjugated to maleylated bovine serum albumin (MBSA) was taken up efficiently through high-affinity MBSA-binding sites on macrophages. Binding of the radiolabeled conjugate to cultured mouse peritoneal macrophages at 4 degrees C was competed for by MBSA but not by PAS. At 37 degrees C, the radiolabeled conjugate was rapidly degraded by the macrophages, leading to release of acid-soluble degradation products in the medium. The drug conjugate was nearly 100 times as effective as free PAS in killing the intracellular mycobacteria in mouse peritoneal macrophages infected in culture with Mycobacterium tuberculosis. The killing of intracellular mycobacteria mediated by the drug conjugate was effectively prevented by simultaneous addition of excess MBSA (100 micrograms/ml) or chloroquine (3 microM) to the medium, whereas these agents did not affect the microbicidal action of free PAS. These results suggest that (i) uptake of the PAS-MBSA conjugate was mediated by cell surface receptors on macrophages which recognize MBSA and (ii) lysosomal hydrolysis of the internalized conjugate resulted in intracellular release of a pharmacologically active form of the drug, which led to selective killing of the M. tuberculosis harbored by mouse macrophages infected in culture. This receptor-mediated modality of delivering drugs to macrophages could contribute to greater therapeutic efficacy and minimization of toxic side effects in the management of tuberculosis and other intracellular mycobacterial infections.
Majumdar
S
S
Institute of Microbial Technology, Chandigarh, India.
Basu
S K
SK
D000418:Albumins / Q000008:administration & dosage* / Q000378:metabolism / Q000493:pharmacokinetics; D010131:Aminosalicylic Acid / Q000008:administration & dosage* / Q000378:metabolism / Q000493:pharmacokinetics; D000818:Animals; D001665:Binding Sites; D004337:Drug Carriers; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism; D007457:Iodine Radioisotopes; D008264:Macrophages / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D010529:Peritoneal Cavity / Q000166:cytology; D014677:Pharmaceutical Vehicles; D012710:Serum Albumin, Bovine; D014376:Tuberculosis / Q000188:drug therapy
D000418:Albumins; D004337:Drug Carriers; D007457:Iodine Radioisotopes; D014677:Pharmaceutical Vehicles; C049877:maleylalbumin; D012710:Serum Albumin, Bovine; D010131:Aminosalicylic Acid