ELISA screening of staphylococcal enterotoxins by means of a specially developed test kit.
The development of a new test kit for the detection of staphylococcal enterotoxins is described. This polyclonal antibody-based enzyme-linked immunosorbent assay (ELISA) is designed for qualitative and quantitative rapid detection of toxin serotypes A, B, C (subgroup 1), D and E in one test system. Results are obtained in 60 min. The lower limit of detection is 0.5 ng/ml; the precision is 90%.
Kraatz-Wadsack
G
G
Institute for Microbiology, Federal Armed Forces Medical Academy, Munich, F.R.G.
Ahrens
W
W
von Stuckrad
J
J
D000907:Antibodies, Bacterial; D003470:Culture Media; D004768:Enterotoxins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus
D000907:Antibodies, Bacterial; D003470:Culture Media; D004768:Enterotoxins; D011933:Reagent Kits, Diagnostic