[An injury of the liver caused by ischemia-reperfusion in rat liver].
Cellular damage of various organs by ischemia following reperfusion is assumed to be at least in part due to lipid peroxidation in biomembranes, and oxygen-derived free radicals play a major role. The level of lipid peroxides in liver tissue increased during 90-min ischemia. When reflow of hepatic blood was allowed, a greater increase in the lipid peroxides was observed. Similar increases were obtained in several serum markers (GOT, GPT and LDH) during the period of ischemia or ischemia-reperfusion. In addition, levels of cytochrome p-450 and NADPH cyt. c reductase activity decreased in proportion to the decrease in microsomal proteins during ischemia or ischemia-reperfusion. On the other hand, superoxide dismutase in blood was significantly increased by ischemia-reperfusion. Rats died within 2 days after liver ischemia of 90 min, while all animals subjected to 30-min ischemia survived. Histopathological examinations indicated that extensive coagulation with erythrocytes occurred and the extent was dependent on the time of ischemia. The liver injury by ischemia-reperfusion could be a useful experimental model for studying liver injury induced by free radicals, for developing hepatoprotective drugs, or for investigating liver transplantation.
Egashira
T
T
Department of Pharmacology, Medical College of Oita, Japan.
Nagai
T
T
Kimba
Y
Y
Murayama
F
F
Goto
S
S
Kudo
Y
Y
Sudo
S
S
Kono
T
T
Yamanaka
Y
Y
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004195:Disease Models, Animal; D005609:Free Radicals; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000378:metabolism; D008107:Liver Diseases / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D013482:Superoxide Dismutase / Q000097:blood; D013997:Time Factors
D005609:Free Radicals; D008054:Lipid Peroxides; D003577:Cytochrome P-450 Enzyme System; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; D009251:NADPH-Ferrihemoprotein Reductase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase