title: Stabilization of clathrin coated vesicles by amantadine, tromantadine and other hydrophobic amines.
abstract: Amantadine and related compounds stabilized the structure of purified pig brain clathrin coated vesicles (CCV) at biologically relevant concentrations. Incubation of purified CCV for 30 min at 25 degrees C or 37 degrees C caused the release of clathrin, as determined by a centrifugation assay, and a reduction in the number of coated vesicles, by electron microscopy. Amantadine (10 mM), tromantadine (1 mM), amidine D295 (cyclohexylcarboximidamide-(N-benzyl)hydrochloride (10 mM), chloroquine (0.1 mM) and monodansylcadaverine (10 mM) significantly reduced the extent of dissociation.
authors.affiliation: Department of Microbiology and Immunology, N.E. Ohio Universities College of Medicine, Rootstown 44272.
authors.forename: Y
authors.initials: Y
authors.lastname: Phonphok
authors.forename: K S
authors.initials: KS
authors.lastname: Rosenthal
mesh_terms: D000547:Amantadine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000588:Amines / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000378:metabolism; D002966:Clathrin / Q000378:metabolism*; D003034:Coated Pits, Cell-Membrane / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D013329:Structure-Activity Relationship; D013552:Swine
chemical_list: D000588:Amines; D002966:Clathrin; D000547:Amantadine; C004279:tromantadine