title: Isolation and characterization of catabolite repression control mutants of Pseudomonas aeruginosa PAO.
abstract: Independently controlled, inducible, catabolic genes in Pseudomonas aeruginosa are subject to strong catabolite repression control by intermediates of the tricarboxylic acid cycle. Mutants which exhibited a pleiotropic loss of catabolite repression control of multiple pathways were isolated. The mutations mapped in the 11-min region of the P. aeruginosa chromosome near argB and pyrE and were designated crc. Crc- mutants no longer showed repression of mannitol and glucose transport, glucose-6-phosphate dehydrogenase, glucokinase, Entner-Doudoroff dehydratase and aldolase, and amidase when grown in the presence of succinate plus an inducer. These activities were not expressed constitutively in Crc- mutants but exhibited wild-type inducible expression.
authors.affiliation: Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond 23298.
authors.forename: J A
authors.initials: JA
authors.lastname: Wolff
authors.forename: C H
authors.initials: CH
authors.lastname: MacGregor
authors.forename: R C
authors.initials: RC
authors.lastname: Eisenberg
authors.forename: P V
authors.initials: PV
authors.lastname: Phibbs
mesh_terms: D000577:Amides / Q000378:metabolism; D000581:Amidohydrolases / Q000096:biosynthesis / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001692:Biological Transport; D002952:Citric Acid Cycle; D003227:Conjugation, Genetic; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism; D008353:Mannitol / Q000378:metabolism; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D012097:Repressor Proteins / Q000235:genetics*; D013386:Succinates / Q000378:metabolism; D014161:Transduction, Genetic
chemical_list: D000577:Amides; D001426:Bacterial Proteins; D012097:Repressor Proteins; D013386:Succinates; D008353:Mannitol; C013083:lactamide; D000581:Amidohydrolases; D005947:Glucose