Leiomyoma cells with 12q15 aberrations can be transformed in vitro and show a relatively stable karyotype during precrisis period.
Tumor cells from three uterine leiomyomas showing translocations involving 12q14-15 were transformed by transfection using the "early regions" of the SV40 genome. The cells had a higher proliferative capacity, were able to form colonies in soft agar, and showed an increased growth potential. Karyotype analyses of these transformed leiomyoma cells showed that the cells had retained the initial t(12;14) and t(12;15).
Stern
C
C
Center of Human Genetics and Genetic Counselling, University of Bremen, Fed. Republic of Germany.
Kazmierczak
B
B
Thode
B
B
Rommel
B
B
Bartnitzke
S
S
Dal Cin
P
P
van de Ven
W
W
Van den Berghe
H
H
Bullerdiek
J
J
D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D015139:Blotting, Southern; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology*; D002869:Chromosome Aberrations / Q000235:genetics / Q000473:pathology*; D002871:Chromosome Banding; D025063:Chromosome Disorders; D002881:Chromosomes, Human, Pair 12; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006801:Humans; D007889:Leiomyoma / Q000235:genetics / Q000473:pathology*; D014162:Transfection; D014594:Uterine Neoplasms / Q000235:genetics / Q000473:pathology
D000952:Antigens, Polyomavirus Transforming; D004273:DNA, Neoplasm