Influence of beta-myrcene on sister-chromatid exchanges induced by mutagens in V79 and HTC cells.
The influence of beta-myrcene (MC) on sister-chromatid exchanges (SCE) in V79 cells induced by 4 S9 mix-activated indirect mutagens was studied. The mutagens used were cyclophosphamide (CP), benzo[a]pyrene (BP), aflatoxin B1 (AFB) and 9,10-dimethyl-1,2-benz[a]anthracene (DMBA). MC effectively inhibited SCEs induced by CP and AFB in a dose-dependent manner, but it had no effect on SCE induction by BP and DMBA. MC also reduced CP-induced SCE frequencies in a hepatic tumor cell line (HTC). These cells are metabolically competent and activate CP into its biologically active metabolites. Our results support the suggestion that MC modulates the genotoxicity of indirect-acting mutagens by inhibiting certain forms of the cytochrome P-450 enzymes required for activation of premutagens like CP and AFB.
Röscheisen
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Abteilung Klinische Genetik, Universität Ulm, F.R.G.
Zamith
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H
Paumgartten
F J
FJ
Speit
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D015127:9,10-Dimethyl-1,2-benzanthracene / Q000378:metabolism / Q000633:toxicity; D000080462:Acyclic Monoterpenes; D016604:Aflatoxin B1; D000348:Aflatoxins / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000633:toxicity; D000818:Animals; D001564:Benzo(a)pyrene / Q000494:pharmacology; D001711:Biotransformation; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D003520:Cyclophosphamide / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000633:toxicity; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D039821:Monoterpenes; D009153:Mutagens / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013729:Terpenes / Q000494:pharmacology*
D000080462:Acyclic Monoterpenes; D000348:Aflatoxins; D065607:Cytochrome P-450 Enzyme Inhibitors; D039821:Monoterpenes; D009153:Mutagens; D013729:Terpenes; C008574:beta-myrcene; D001564:Benzo(a)pyrene; D015127:9,10-Dimethyl-1,2-benzanthracene; D003520:Cyclophosphamide; D003577:Cytochrome P-450 Enzyme System; D016604:Aflatoxin B1