Ribosomal DNA fingerprinting of Listeria monocytogenes using a digoxigenin-labeled DNA probe.
A nonisotopic ribosomal DNA fingerprinting technique was developed for the characterization of Listeria monocytogenes. Plasmid pKK3535 (a pBR322-derived plasmid containing rrnB ribosomal RNA operon of Escherichia coli) was labeled with digoxigenin-11-dUTP by random priming and used to probe EcoRI fragments of L. monocytogenes chromosomal DNA on nylon filters. The method was successfully applied to the characterization of two sets of patient and food isolates of L. monocytogenes.
Graves
L M
LM
Meningitis and Special Pathogens Branch, Centers for Disease Control, Atlanta, Georgia 30333.
Swaminathan
B
B
Reeves
M W
MW
Wenger
J
J
D016172:DNA Fingerprinting / Q000379:methods*; D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004076:Digoxigenin; D005517:Foodborne Diseases / Q000382:microbiology; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics*; D008088:Listeriosis / Q000382:microbiology
D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004076:Digoxigenin