Cell cycle kinetics of human anagen scalp hair bulbs in thyroid disorders determined by DNA flow cytometry.
In both, 6 hyperthyroid and 6 hypothyroid patients as well as 10 healthy volunteers, cell cycle kinetics of dissected anagen scalp hair bulbs were determined by means of DNA flow cytometry (DNA-FCM). Compared with the healthy control group in patients with thyroid disorders striking differences of cell kinetic data were evaluated. In hyperthyroidism a significant increase (30%) and in hypothyroidism a significant decrease (15%) of S and G2+M phase cell percentages was found. The proliferation index (S+G2+M %) calculated revealed similar results. A correlation between the height of S phase percentages and plasma T3 levels was recognizable but could not be proven statistically. By means of DNA-FCM the study demonstrates for the first time the influence of thyroid hormones on in vivo cell cycle kinetics of human scalp hair bulbs.
Schell
H
H
Department of Dermatology, University of Erlangen-Nürnberg, FRG.
Kiesewetter
F
F
Seidel
C
C
von Hintzenstern
J
J
D000328:Adult; D001706:Biopsy; D002453:Cell Cycle; D002455:Cell Division; D004247:DNA; D005434:Flow Cytometry; D006197:Hair / Q000166:cytology*; D006801:Humans; D006980:Hyperthyroidism / Q000473:pathology*; D007037:Hypothyroidism / Q000473:pathology*; D016196:S Phase; D013974:Thyroxine / Q000097:blood; D013975:Thyroxine-Binding Proteins / Q000032:analysis; D014284:Triiodothyronine / Q000097:blood
D013975:Thyroxine-Binding Proteins; D014284:Triiodothyronine; D004247:DNA; D013974:Thyroxine