title: Corticosteroid effects on lipid lateral diffusion in CEM-C1 and CEM-C7 acute lymphoblastic leukemia cells.
abstract: We have examined glucocorticoid effects on CEM-C7 and CEM-C1 subclones of a leukemic human T-cell line using fluorescence photobleaching recovery techniques. Incubation with 10(-5) M triamcinolone acetonide (TA) increased lipid lateral diffusion on steroid-sensitive CEM-C7 cells but had no effect on steroid-resistant CEM-C1 cells. CEM-C7 cells incubated in serum-free medium responded only to TA but, when fetal calf serum was added to the incubation medium, would also respond to 10(-5) M dexamethasone and hydrocortisone. Thus, glucocorticoids can cause increased lipid lateral diffusion in CEM-C7 cells, while having no effect on steroid-resistant CEM-C1 cells.
authors.affiliation: University of Colorado Health Sciences Center School of Medicine, Denver.
authors.forename: C J
authors.initials: CJ
authors.lastname: Bulva
authors.forename: J
authors.initials: J
authors.lastname: Printen
authors.forename: G
authors.initials: G
authors.lastname: Melnykovych
authors.forename: L
authors.initials: L
authors.lastname: Cutts
authors.forename: B G
authors.initials: BG
authors.lastname: Barisas
authors.forename: D A
authors.initials: DA
authors.lastname: Roess
mesh_terms: D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology; D004058:Diffusion; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D007945:Leukemia, Lymphoid / Q000188:drug therapy / Q000378:metabolism*; D050356:Lipid Metabolism; D014222:Triamcinolone Acetonide / Q000494:pharmacology
chemical_list: D005938:Glucocorticoids; D003907:Dexamethasone; D014222:Triamcinolone Acetonide; D006854:Hydrocortisone