title: Histochemistry of tartrate-resistant acid phosphatase and carbonic anhydrase isoenzyme II in osteoclast-like giant cells in bone tumours.
abstract: Using routinely processed, paraffin-embedded tissue specimens, osteoclast-like giant cells in giant cell tumour of bone (GCT), chondroblastoma, osteoblastoma and osteoblastic osteosarcoma were examined histochemically for osteoclast-specific enzymes tartrate-resistant acid phosphatase (TRAP) and carbonic anhydrase isoenzyme II (CA-II). Osteoclast-like giant cells and some mononuclear cells possessed TRAP activity. These were further classified with respect to CA-II immunoreactivity, i.e. cells with CA-II were seen in GCT and chondroblastoma, while those in osteoblastoma and osteoblastic osteosarcoma were negative for CA-II. All the cellular components in malignant fibrous histiocytoma and various extraosseous inflammatory lesions including malignant giant cells and macrophage polykaryons were negative for both TRAP and CA-II. These results indicate that osteoclast-like giant cells in GCT, chondroblastoma, osteoblastoma and osteoblastic osteosarcoma are all osteoclasts and generated by fusion of mononuclear cells with the same histochemical characteristics as osteoclast-like giant cells. The difference in CA-II immunoreactivity suggests the functional or maturational difference between osteoclast-like giant cells in GCT and chondroblastoma and those in osteoblastoma and osteosarcoma.
authors.affiliation: Department of Oral Pathology, Osaka University Faculty of Dentistry, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Toyosawa
authors.forename: Y
authors.initials: Y
authors.lastname: Ogawa
authors.forename: C K
authors.initials: CK
authors.lastname: Chang
authors.forename: S S
authors.initials: SS
authors.lastname: Hong
authors.forename: T
authors.initials: T
authors.lastname: Yagi
authors.forename: H
authors.initials: H
authors.lastname: Kuwahara
authors.forename: K
authors.initials: K
authors.lastname: Wakasa
authors.forename: M
authors.initials: M
authors.lastname: Sakurai
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology; D002256:Carbonic Anhydrases / Q000378:metabolism*; D004351:Drug Resistance; D015726:Giant Cells / Q000378:metabolism* / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D010010:Osteoclasts / Q000378:metabolism* / Q000473:pathology; D013644:Tartrates / Q000494:pharmacology*
chemical_list: D013644:Tartrates; D000135:Acid Phosphatase; D002256:Carbonic Anhydrases; C029768:tartaric acid