Localization of CD44 (P80) on the external surface of a human astrocytoma cell.
A brain antigen, originally identified by a MAb 44D10, has been shown to be a glycoprotein with an Mr of 80 kDa. Cellular localization studies of sections of brain showed that the antigen was associated with the membrane of astrocytes. In the present study we demonstrate its localization to the membrane of an astrocytoma cell line by fluorescence and electron microscopic immunogold methods. Heavy labelling with immunogold was found along cellular processes. Labelling of the smooth surfaces of cells and the microvilli was also observed.
Girgrah
N
N
Department of Biochemistry, Hospital for Sick Children, Toronto, Ontario, Canada.
Ackerley
C A
CA
Moscarello
M A
MA
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis*; D001254:Astrocytoma; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure*; D016650:Fluorescein-5-isothiocyanate; D005455:Fluorescent Antibody Technique; D006801:Humans; D016253:Microscopy, Immunoelectron; D016201:Receptors, Lymphocyte Homing / Q000032:analysis*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D016201:Receptors, Lymphocyte Homing; D016650:Fluorescein-5-isothiocyanate