title: Separation of carbohydrates and carbohydrate derivatives by HPLC with cation-exchange columns at high pH.
abstract: Cation-exchange columns were found to be stable when used at high pH and high temperature for high-performance liquid chromatographic separations of carbohydrates and carbohydrate derivatives. Pulsed amperometric detection and refractive index detection were found to be suitable detection modes with these cation-exchange columns. Significant differences in carbohydrate separation selectivity were observed between cation-exchange and anion-exchange columns.
authors.affiliation: Interaction Chemicals Inc., Mountain View, CA 94043.
authors.forename: D W
authors.initials: DW
authors.lastname: Togami
authors.forename: B J
authors.initials: BJ
authors.lastname: Poulsen
authors.forename: C W
authors.initials: CW
authors.lastname: Batalao
authors.forename: W A
authors.initials: WA
authors.lastname: Rolls
mesh_terms: D002241:Carbohydrates / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D013696:Temperature
chemical_list: D002241:Carbohydrates