Primitive erythropoiesis of mouse teratocarcinoma stem cells PCC3/A/1 in serum-free medium.
Mouse teratocarcinoma stem cells PCC3/A/1 differentiated into various types of cells, such as red cells, when they were grown in serum-free medium containing transferrin and bovine serum albumin on a KCF cell feeder layer. These red cells were stained well with 2,7-diaminofluorene (DAF), and therefore were erythroid cells. They were nucleated and contained embryonic globin chains, immunologically identified with antiembryonic hemoglobin antisera after acid urea Triton X-100 polyacrylamide gel electrophoresis (UT-PAGE). The addition of erythropoietin to the culture medium enhanced the production of both embryonic and adult globin chains. The addition of interleukin-3 also enhanced the production of embryonic globin chains, but not the production of adult globin chains. These results indicated that primitive erythropoiesis of PCC3/A/1 teratocarcinoma cells did not require exogenous addition of any hematopoietic factor such as erythropoietin or interleukin-3. This culture system will be a new model system for investigating the factors regulating the primitive erythropoiesis in yolk sac blood islands.
Miwa
Y
Y
Laboratory of Molecular Oncology, Tsukuba Life Science Center, Institute of Physical and Chemical Research, Ibaraki, Japan.
Atsumi
T
T
Imai
N
N
Ikawa
Y
Y
D000818:Animals; D002454:Cell Differentiation; D003470:Culture Media; D004920:Erythropoiesis; D005914:Globins / Q000032:analysis; D051379:Mice; D008954:Models, Biological; D013724:Teratoma / Q000648:ultrastructure; D014407:Tumor Cells, Cultured / Q000502:physiology* / Q000648:ultrastructure
D003470:Culture Media; D005914:Globins