Fibroblast growth factor-induced increased survival of cholinergic mesopontine neurons in culture.
Basic fibroblast growth factor (bFGF) was found to increase the survival of immunocytochemically-identified cholinergic mesopontine neurons in dissociated cell cultures of embryonic rat midbrain. In contrast, cultures exposed to, (a) bFGF and an antibody to bFGF, (b) antibody to bFGF alone, or (c) untreated, contained approximately half the number of cholinergic neurons compared to bFGF-treated cultures.
Garcia-Rill
E
E
Department of Anatomy, University of Arkansas for Medical Sciences, Little Rock 72205.
Davies
D L
DL
Skinner
R D
RD
Biedermann
J A
JA
McHalffey
C
C
D000818:Animals; D000911:Antibodies, Monoclonal; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002795:Choline O-Acetyltransferase / Q000032:analysis*; D004622:Embryo, Mammalian; D016222:Fibroblast Growth Factor 2 / Q000276:immunology / Q000494:pharmacology*; D005865:Gestational Age; D007150:Immunohistochemistry; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D011149:Pons / Q000166:cytology* / Q000201:enzymology; D051381:Rats
D000911:Antibodies, Monoclonal; D016222:Fibroblast Growth Factor 2; D002795:Choline O-Acetyltransferase