title: Pleiotropic changes resulting from depletion of Era, an essential GTP-binding protein in Escherichia coli.
abstract: Phenotypic analysis of a temperature-sensitive era mutant strain indicates that Escherichia coli cells depleted of Era undergo many physiological changes. At 43 degrees C, a completely non-permissive temperature, growth is arrested because of loss of the gene and depletion of the Era protein. Depletion of Era at 43 degrees C results in depressed synthesis of heat-shock proteins DnaK, GroEL/ES, D33.4 and C62.5, lack of thermal induction of ppGpp pool levels, and increased capacity for carbon source metabolism through the citric acid cycle. Thus, in addition to inhibition of cell growth and viability, loss of Era function results in pleiotropic changes including abnormal adaptation to thermal stress.
authors.affiliation: Department of Biochemistry, Robert Wood Johnson Medical School, Rutgers University of Medicine and Dentistry of New Jersey, Piscataway 08854-5635.
authors.forename: C G
authors.initials: CG
authors.lastname: Lerner
authors.forename: M
authors.initials: M
authors.lastname: Inouye
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002455:Cell Division; D002952:Citric Acid Cycle; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D029968:Escherichia coli Proteins; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006159:Guanosine Tetraphosphate / Q000096:biosynthesis; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D007700:Kinetics; D009154:Mutation; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D016601:RNA-Binding Proteins; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D016601:RNA-Binding Proteins; C068027:era protein, E coli; D006159:Guanosine Tetraphosphate; D019204:GTP-Binding Proteins