Detection apparatus for multiple heterogeneous chemiluminescence immunoassay configurations.
We describe an apparatus for measuring signals emanated from two heterogeneous chemiluminescence immunoassay (CLIA) configurations: antibody-coated polystyrene beads, in reaction tray wells, and microparticles captured by a porous matrix. An optics and fluidics design which allows the use of a common detection head for these two different assay configurations is described. The detection head moves along three Cartesian coordinates to create a localized light-tight compartment around each individual disposable reaction vessel. Reproducibility of the light seal, trigger solution delivery, and mixing is achieved for acridinium-labeled CLIA. The coated polystyrene beads configuration is tested using beta HCG, CEA, and TSH assays. The microparticle-capture configuration is tested using beta HCG and HBsAg assays. The microparticle capture CLIA has shorter incubation times and the potential for ease of automation.
Khalil
O S
OS
Diagnostics Division, Abbott Laboratories, Abbott Park, Illinois 60064.
Zurek
T F
TF
Pepe
C
C
Genger
K
K
Huff
D G
DG
Coleman
C
C
Hanna
C
C
Hu
R
R
Mackowiack
J
J
Bennett
L
L
D003627:Data Interpretation, Statistical; D004867:Equipment Design; D006511:Hepatitis B Antigens / Q000032:analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D008163:Luminescent Measurements; D011137:Polystyrenes; D012044:Regression Analysis
D006511:Hepatitis B Antigens; D011137:Polystyrenes