Isolation of a cathepsin B-encoding cDNA from murine osteogenic cells.
Cathepsin B-encoding cDNA (CTSB) clones have been isolated from a lambda gt10 library of a murine osteosarcoma by differential screening during a search for genes which are typically expressed during osteogenic differentiation in mouse mandibular condyles in vitro. Sequencing of the CTSB 3' end revealed that the isolated sequence contained an 825-bp 3'-noncoding region, the polyadenylation signal and the poly(A) tail. The enhanced CTSB expression during the early stages of the enchondral ossification-like process in mandibular condyles in vitro suggests that CTSB participates in the degradation of cartilage matrix prior to the synthesis of bone matrix proteins.
Freimert
C
C
GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Abteilung für Molekulare Zellpathologie, Neuherberg F.R.G.
Closs
E I
EI
Silbermann
M
M
Erfle
V
V
Strauss
P G
PG
D000818:Animals; D001483:Base Sequence; D002401:Cathepsin B / Q000235:genetics*; D002454:Cell Differentiation / Q000502:physiology*; D015870:Gene Expression / Q000502:physiology*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010012:Osteogenesis / Q000502:physiology*; D011061:Poly A / Q000235:genetics; D051381:Rats; D015183:Restriction Mapping; D016415:Sequence Alignment; D014407:Tumor Cells, Cultured
D011061:Poly A; D002401:Cathepsin B