title: Nucleotide sequence of the secY gene from Lactococcus lactis and identification of conserved regions by comparison of four SecY proteins.
abstract: Sec Y is an integral membrane protein which participates in the translocation of proteins through the bacterial cell membrane. We have cloned the sec Y gene of Lactococcus lactis, and found its deduced protein sequence, 439 amino acids long, to be similar in length to the previously determined Sec Y proteins of Escherichia coli, Bacillus subtilis and Mycoplasma capricolum. Comparison of the L. lactis Sec Y to the 3 other Sec Y proteins revealed 90 conserved amino acid residues (21%). Nearly half of the conserved residues are clustered in 2 of the 10 transmembrane segments, and in 2 of the 6 cytoplasmic regions. Some of the conserved regions are apparently responsible for the interactions of Sec Y with signal sequences, and the proteins SecE and SecA.
authors.affiliation: Institute of Biotechnology, University of Helsinki, Finland.
authors.forename: T
authors.initials: T
authors.lastname: Koivula
authors.forename: I
authors.initials: I
authors.lastname: Palva
authors.forename: H
authors.initials: H
authors.lastname: Hemilä
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001692:Biological Transport / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D013294:Lactococcus lactis / Q000235:genetics*; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics; D000069816:SEC Translocation Channels; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D000069816:SEC Translocation Channels; C057841:SecY protein, E coli