New trends in the use of Al(OH)3-conjugated endotoxins and their subunits from the S- and R-forms of Shigella dysenteriae serovar 1 for model vaccination purposes.
The lipopolysaccharides, lipopolysaccharide-protein complexes and their lipids A, isolated from Shigella dysenteriae 1, exhibited lethal toxicity (LD50 300-400 micrograms per mouse), pyrogenicity (0.01-1.0 microgram), activity in the Limulus test (10(-3)-10(-12) mg ml-1) and produced a positive local Shwartzman reaction. It was found that these substances bound to Al(OH)3 were capable of successfully protecting mice from challenge with both homologous and heterologous infections (up to 90% of mice survived a challenge with LD100), whereas immunization with substances with no biological carrier exhibited very low or zero protective effects. Furthermore, the positive cross-reactivity of lipids A in the agar immunodiffusion test confirms the wide applicability for vaccination purposes.
Sourek
J
J
Institute of Hygiene and Epidemiology, Prague, Czechoslovakia.
Levin
J
J
Trnka
T
T
Zelenková
L
L
D000536:Aluminum Hydroxide; D000818:Animals; D001428:Bacterial Vaccines / Q000302:isolation & purification* / Q000633:toxicity; D004405:Dysentery, Bacillary / Q000517:prevention & control; D004731:Endotoxins / Q000302:isolation & purification / Q000633:toxicity; D005779:Immunodiffusion; D007928:Lethal Dose 50; D008033:Limulus Test; D051379:Mice; D008813:Mice, Inbred ICR; D011749:Pyrogens; D011817:Rabbits; D012762:Shigella dysenteriae / Q000145:classification / Q000276:immunology*; D012790:Shwartzman Phenomenon
D001428:Bacterial Vaccines; D004731:Endotoxins; D011749:Pyrogens; D000536:Aluminum Hydroxide