title: Effect of basal and pulsatile LH release on FSH-stimulated follicle growth in ewes chronically treated with gonadotrophin-releasing hormone agonist.
abstract: Ewes chronically treated with gonadotrophin-releasing hormone (GnRH) agonist were used to investigate the importance of the peripheral concentration of LH in FSH-stimulated follicular development. Twenty-four Welsh Mountain ewes were treated with two agonist implants containing 3.3 mg buserelin. During week 6 of treatment all the ewes were given a 72-h continuous infusion of ovine FSH alone (3 micrograms/h) or FSH with large (7.5 micrograms)- or small (2.5 micrograms) amplitude pulses of ovine LH delivered at 4-hourly intervals. The importance of baseline LH throughout the FSH infusion was evaluated in six animals which were treated with a specific antiserum against bovine LH (LH-AS) 15-20 h before the start of FSH treatment. In the absence of LH-AS, infusion of FSH alone or with large or small pulses of LH stimulated the development of a normal number of small follicles (less than or equal to 2.5 mm in diameter) and large follicles (greater than 2.5 mm in diameter). These follicles had normal diameter and steroid secretion compared with control ewes on day 8 of the luteal phase. In contrast, the animals pretreated with LH-AS developed no follicles greater than 2.0 mm in diameter but the number of small follicles per ewe was significantly (P less than 0.05) increased. These results support the hypothesis that FSH in the absence of pulsatile LH release stimulates preovulatory follicular development in ewes treated with GnRH agonist. The follicular response to LH pulses of different amplitude is dependent on both the stage of development of the follicle and the peripheral concentration of FSH.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: MRC Reproductive Biology Unit, University of Edinburgh Centre for Reproductive Biology.
authors.forename: H M
authors.initials: HM
authors.lastname: Picton
authors.forename: A S
authors.initials: AS
authors.lastname: McNeilly
mesh_terms: D000818:Animals; D002064:Buserelin / Q000494:pharmacology*; D004334:Drug Administration Schedule; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood / Q000494:pharmacology*; D007106:Immune Sera; D007986:Luteinizing Hormone / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D006080:Ovarian Follicle / Q000254:growth & development*; D012636:Secretory Rate / Q000502:physiology; D012756:Sheep
chemical_list: D007106:Immune Sera; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D002064:Buserelin