Assembly of combinatorial antibody libraries on phage surfaces: the gene III site.
A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carboxyl-terminal domain of the gene III protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 10(3)- to 10(5)-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.
Barbas
C F
CF
Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.
Kang
A S
AS
Lerner
R A
RA
Benkovic
S J
SJ
D000907:Antibodies, Bacterial / Q000235:genetics; D000916:Antibody Diversity; D001483:Base Sequence; D001666:Binding Sites, Antibody; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D015870:Gene Expression; D015723:Gene Library; D005822:Genetic Vectors; D007140:Immunoglobulin Fab Fragments / Q000235:genetics*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D011993:Recombinant Fusion Proteins; D013745:Tetanus Toxoid / Q000276:immunology
D000907:Antibodies, Bacterial; D007140:Immunoglobulin Fab Fragments; D009841:Oligonucleotides; D011993:Recombinant Fusion Proteins; D013745:Tetanus Toxoid