Induction of transplantable tumors by repeated subcutaneous injections of natural and synthetic vitamin E in mice and rats.
Natural vitamin E and synthetic vitamin E (dl-alpha-tocopheryl acetate) were tested for their tumorigenicity in rodents. Transplantable tumors, at the site of injection, were induced by repeated injections of these compounds in two strains of mice, NFS/N and C57BL/6N x C3H/He F1, and in a strain of rats, Fischer 344. Natural vitamin E was tumorigenic in both strains of female mice only when injected with soya oil. In contrast, dl-alpha-tocopheryl acetate alone was capable of inducing tumors in Fischer 344 rats. Only one out of 5 male NFS/N mice given dl-alpha-tocopheryl acetate developed a tumor. Therefore, Fischer 344 rats were more susceptible to tumor formation by dl-alpha-tocopheryl acetate than NFS/N mice. dl-alpha-Tocopheryl acetate with soya oil or with palm oil also resulted in the formation of transplantable tumors in NFS/N mice and Fischer 344 rats. There was no difference in the tumor incidence between mice treated with dl-alpha-tocopheryl acetate alone and dl-alpha-tocopheryl acetate plus soya oil or palm oil. However, in rats, the incidence was lower for a group treated with dl-alpha-tocopheryl acetate plus palm oil than for those with dl-alpha-tocopheryl acetate alone and with dl-alpha-tocopheryl acetate plus soya oil.
Nitta
Y
Y
Department of Pathology, Research Institute for Nuclear Medicine and Biology, Hiroshima University.
Kamiya
K
K
Tanimoto
M
M
Sadamoto
S
S
Niwa
O
O
Yokoro
K
K
D000818:Animals; D005354:Fibrosarcoma / Q000139:chemically induced* / Q000235:genetics / Q000473:pathology; D005784:Gene Amplification; D016259:Genes, myc; D007279:Injections, Subcutaneous; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D000073878:Palm Oil; D010938:Plant Oils / Q000008:administration & dosage; D051381:Rats; D011916:Rats, Inbred F344; D012983:Soft Tissue Neoplasms / Q000139:chemically induced* / Q000235:genetics / Q000473:pathology; D013024:Soybean Oil / Q000008:administration & dosage; D024505:Tocopherols; D014810:Vitamin E / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity*; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
D010938:Plant Oils; D014810:Vitamin E; D000073878:Palm Oil; D013024:Soybean Oil; D024502:alpha-Tocopherol; D024505:Tocopherols