title: Response of insulin-like growth factor I and renal hemodynamics to a high- and low-protein diet in the rat.
abstract: An increase in plasma insulin-like growth factor I (IGF-I) levels by growth hormone injection or IGF-I infusion can raise renal plasma flow and glomerular filtration rate. However, it is not known whether a more physiological stimulus for IGF-I will also increase IGF-I in the kidney and whether the increase in renal or serum IGF-I is correlated with the increase in renal plasma flow and glomerular filtration rate. Male rats were pair fed either a high-protein (36% protein, N = 9) or a low-protein but isocaloric diet (9% protein, N = 9) for 10 to 14 days. Renal plasma flow and glomerular filtration rate were then estimated by clearance measurements, and IGF-I was measured in extracted serum, liver, renal cortical tissue, and glomeruli. Body weight gain and combined kidney weight were higher in high-protein rats as compared with low-protein animals (0.86 +/- 0.02 SEM versus 0.77 +/- 0.02 g/100 g body wt; P less than 0.05), but liver weights were not different. Serum, liver, and glomerular IGF-I levels were also higher in the high-protein rats as compared with the low-protein animals (serum, 1.12 +/- 0.03 versus 0.80 +/- 0.06 U/mL, P less than 0.05; liver, 183 +/- 17 versus 117 +/- 16 mU/g wet wt, P less than 0.05; glomeruli, 7.43 +/- 0.73 versus 4.81 +/- 0.59 mU/mg of protein, P less than 0.05). In contrast, the renal cortical IGF-I levels were not different in high-protein versus low-protein rats.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Division of Nephrology and Hypertension, Harbor-UCLA Medical Center, Torrance 90505.
authors.forename: R
authors.initials: R
authors.lastname: Hirschberg
authors.forename: J D
authors.initials: JD
authors.lastname: Kopple
mesh_terms: D000818:Animals; D004044:Dietary Proteins / Q000008:administration & dosage*; D005919:Glomerular Filtration Rate / Q000502:physiology; D006984:Hypertrophy; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012079:Renal Circulation / Q000502:physiology*; D014655:Vascular Resistance / Q000502:physiology
chemical_list: D004044:Dietary Proteins; D007334:Insulin-Like Growth Factor I