Construction of a family of lactococcal vectors for gene cloning and translational fusion.
A family of stable lactococcal vectors have been constructed based on the pFX1 replicon using either the alpha fragment or the complete Escherichia coli lacZ gene as a selective marker. These vectors also incorporate multiple cloning sites and examples are given of their use for gene cloning and translational fusion studies in lactococci.
Xu
F F
FF
Department of Biotechnology, Massey University, Palmerston North, New Zealand.
Pearce
L E
LE
Yu
P L
PL
D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005822:Genetic Vectors; D007763:Lac Operon; D013294:Lactococcus lactis / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D014176:Protein Biosynthesis; D012093:Replicon; D015183:Restriction Mapping