High-performance liquid chromatographic separation of racemic and diastereomeric mixtures of 2,4-pentadienoate-iron tricarbonyl derivatives.
beta-Cyclodextrin chiral stationary phase facilitates the chiral separation of the (+/-)-methyl-5-formyl-2,4-pentadienoate-iron tricarbonyl (1) racemic mixture. The separation of oxazolidine derivatives 2 and 3 diastereomers were achieved with a C18 column but the compounds underwent in-column hydrolysis to give (-)- and (+)-1, respectively. This hydrolysis was exploited for the determination of 2 and 3 by the beta-cyclodextrin column, namely 2 and 3 were initially and completely hydrolyzed in the column to give (-)- and (+)-1 and this racemic mixture was then separated by this chiral column.
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Department of Chemistry, Marquette University, Milwaukee, WI 53233.
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D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000302:isolation & purification*; D005290:Ferric Compounds / Q000302:isolation & purification*; D007202:Indicators and Reagents; D013237:Stereoisomerism
D003505:Cyclodextrins; D005231:Fatty Acids, Unsaturated; D005290:Ferric Compounds; D007202:Indicators and Reagents; C070663:methyl 5-formyl-2,4-pentadienoate-iron tricarbonyl*