title: Neither the endogenous nor a functional steroid hormone receptor binding site transactivate the ribosomal RNA gene promoter in vitro.
abstract: The mammalian ribosomal RNA gene promoters exhibit a conserved sequence between positions +1 and +16 that shows a high degree of homology to the response element for glucocorticoids and progestins (GRE/PRE). These sequences bind specifically the glucocorticoid receptor and the progesterone receptor (PR) albeit with lower affinity than a canonical GRE/PRE. Because steroid hormones are known to affect expression of the ribosomal genes, we tested the influence of hormone receptors on the activity of the ribosomal RNA gene promoter in a cell-free transcription assay. Preparations of PR that induce transcription from the mouse mammary tumour virus (MMTV) promoter do not stimulate but slightly inhibit transcription from the ribosomal RNA gene promoter. This weak negative effect is not mediated through binding to the hypothetical GRE/PRE as a mutant promoter that does not bind receptor is equally repressed. Introduction of the functional MMTV GRE/PRE upstream of the basal ribosomal RNA gene promoter does not enhance its transcription in the presence of an active PR. Thus, RNA polymerase I transcription cannot be stimulated in vitro by cis elements and regulatory proteins that are active in RNA polymerase II transcription.
authors.affiliation: Institut für Molekularbiologie und Tumorforschung, Philipps-Universität, Marburg, Fed. Rep. Germany.
authors.forename: A
authors.initials: A
authors.lastname: Misseyanni
authors.forename: C
authors.initials: C
authors.lastname: Scheidereit
authors.forename: M
authors.initials: M
authors.lastname: Kalff
authors.forename: M
authors.initials: M
authors.lastname: Beato
mesh_terms: D000818:Animals; D016375:Antisense Elements (Genetics); D001483:Base Sequence; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics*; D003850:Deoxyribonuclease I; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015347:RNA Probes; D012335:RNA, Ribosomal / Q000235:genetics*; D011817:Rabbits; D011987:Receptors, Steroid / Q000378:metabolism*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014599:Uterus / Q000502:physiology
chemical_list: D016375:Antisense Elements (Genetics); D004275:DNA, Ribosomal; D015347:RNA Probes; D012335:RNA, Ribosomal; D011987:Receptors, Steroid; D003850:Deoxyribonuclease I