GM-CSF and IL-3 stimulate diacylglycerol generation in murine bone marrow-derived macrophages.
In murine bone marrow-derived macrophages, prelabeled with either [3H]myristic acid or [3H]arachidonic acid, the mitogenic colony stimulating factors GM-CSF and IL-3 stimulated a transient increase in [3H]diacylglycerol generation. Maximum [3H]diacylglycerol levels were detected at 10-15 min. The stimulation of [3H]diacylglycerol generation was dependent on the concentration of CSF and correlated with their ability to activate a variety of processes in the macrophage, including DNA synthesis. This is the first report to demonstrate that GM-CSF elevates diacylglycerol levels in macrophages and also to show that diacylglycerol generation may be an important signaling mechanism for IL-3 action. In conjunction with our recent demonstration that the mitogenic agents CSF-1, 12-0-tetradecanoylphorbol-13-acetate and exogenous phospholipase C also stimulate diacylglycerol generation in the macrophage (Veis and Hamilton, J.Cell.Physiol., 147, 298-305, 1991), our findings suggest that an increase in diacylglycerol levels is necessary but not sufficient for macrophage proliferation.
Veis
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University of Melbourne, Department of Medicine, Royal Melbourne Hospital, Parkville, Victoria, Australia.
Hamilton
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JA
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001853:Bone Marrow / Q000378:metabolism*; D002478:Cells, Cultured; D004075:Diglycerides / Q000096:biosynthesis* / Q000302:isolation & purification; D004305:Dose-Response Relationship, Drug; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology*; D007700:Kinetics; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology
D001095:Arachidonic Acids; D004075:Diglycerides; D007377:Interleukin-3; D009227:Myristic Acids; D011994:Recombinant Proteins; D019814:Myristic Acid; D016718:Arachidonic Acid; D016178:Granulocyte-Macrophage Colony-Stimulating Factor