Construction of scrA::lacZ gene fusions to investigate regulation of the sucrose PTS of Streptococcus mutans.
The scrA gene coding for sucrose EnzymeII of the phosphoenolpyruvate dependent phosphotransferase system previously isolated from Streptococcus mutans was fused in vitro to the promoterless lacZ' gene to monitor the expression of the scrA gene. The scrA::lacZ gene fusion was introduced back into S. mutans GS-5IS3 by two independent transformation procedures involving either linear or plasmid DNA to produce both scrA and scrA+ mutants. These mutants should prove useful for analyzing the regulation of sucrose transport in S. mutans.
Sato
Y
Y
Department of Biochemistry, Tokyo Dental College, Chiba, Japan.
Yamamoto
Y
Y
Suzuki
R
R
Kizaki
H
H
Kuramitsu
H K
HK
D001693:Biological Transport, Active; D015139:Blotting, Southern; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics; D015183:Restriction Mapping; D013295:Streptococcus mutans / Q000235:genetics* / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism*; D014169:Transformation, Bacterial; D001616:beta-Galactosidase / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D013395:Sucrose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C055701:phosphoenolpyruvate-sucrose phosphotransferase; D001616:beta-Galactosidase