title: A coat subunit of Golgi-derived non-clathrin-coated vesicles with homology to the clathrin-coated vesicle coat protein beta-adaptin.
abstract: Four high-molecular-weight proteins form the main subunits of the coat of Golgi-derived (non-clathrin) coated vesicles. One of these coat proteins, beta-COP, is identical to a Golgi-associated protein of relative mass 110,000 (110K) that shares homology with the adaptin proteins of clathrin-coated vesicles. This connection, and the comparable molecular weights of the coat proteins of Golgi-derived and clathrin-coated vesicles, indicates that they may be structurally related. The identification of beta-COP as the 110K protein explains the blocking of secretion by the drug brefeldin A.
authors.affiliation: Department of Biochemistry, Stanford University, California 94305.
authors.forename: T
authors.initials: T
authors.lastname: Serafini
authors.forename: G
authors.initials: G
authors.lastname: Stenbeck
authors.forename: A
authors.initials: A
authors.lastname: Brecht
authors.forename: F
authors.initials: F
authors.lastname: Lottspeich
authors.forename: L
authors.initials: L
authors.lastname: Orci
authors.forename: J E
authors.initials: JE
authors.lastname: Rothman
authors.forename: F T
authors.initials: FT
authors.lastname: Wieland
mesh_terms: D033966:Adaptor Protein Complex beta Subunits; D000595:Amino Acid Sequence; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002966:Clathrin / Q000737:chemistry*; D003034:Coated Pits, Cell-Membrane / Q000737:chemistry* / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
chemical_list: D033966:Adaptor Protein Complex beta Subunits; D002966:Clathrin; D008565:Membrane Proteins; D011506:Proteins