An NAD-specific glutamate dehydrogenase from cyanobacteria. Identification and properties.
The unicellular cyanobacterium Synechocystis sp. PCC 6803 presents a hexameric NAD-specific glutamate dehydrogenase with a molecular mass of 295 kDa. The enzyme differs from the NADP-glutamate dehydrogenase found in the same strain and is coded by a different gene. NAD-glutamate dehydrogenase shows a high coenzyme specificity, catalyzes preferentially glutamate formation and presents Km values for ammonium, NADH and 2-oxoglutarate of 4.5 mM, 50 microM and 1.8 mM respectively. An animating role for the enzyme is discussed.
Chávez
S
S
Departamento de Bioquimica Vegetal y Biologia Molecular, Universidad de Sevilla y CSIC, Spain.
Candau
P
P
D000641:Ammonia / Q000378:metabolism*; D002850:Chromatography, Gel; D000458:Cyanobacteria / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005969:Glutamate Dehydrogenase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007527:Isoenzymes; D007700:Kinetics; D009243:NAD / Q000378:metabolism
D007527:Isoenzymes; D009243:NAD; D000641:Ammonia; D005969:Glutamate Dehydrogenase