title: Low incidence of Ha-ras oncogene mutations in human epidermal tumors.
abstract: Activation of the Ha-ras oncogene by point mutations has been suggested to play a role in animal skin carcinogenesis models. In this study, we investigated the significance of the Ha-ras mutations in human epidermal tumors. DNAs from paraffin-embedded tissues of benign and malignant human epidermal tumors (27 samples from 25 patients) were prepared and examined for point mutations of codons 12, 13 and 61 of Ha-ras gene by polymerase chain reaction and oligonucleotide hybridization. Only one sample of basal cell carcinoma and one sample of keratoacanthoma were found to carry an A to T transversion at the second position of codon 61. This low incidence of Ha-ras mutations suggests that the mutational activation of the gene may not be primarily involved in human epidermal tumorigenesis.
authors.affiliation: Department of Dermatology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: F M
authors.initials: FM
authors.lastname: Lieu
authors.forename: K
authors.initials: K
authors.lastname: Yamanishi
authors.forename: K
authors.initials: K
authors.lastname: Konishi
authors.forename: S
authors.initials: S
authors.lastname: Kishimoto
authors.forename: H
authors.initials: H
authors.lastname: Yasuno
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D004252:DNA Mutational Analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry; D009857:Oncogenes; D016133:Polymerase Chain Reaction; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics*; D012878:Skin Neoplasms / Q000235:genetics*
chemical_list: D009841:Oligonucleotides; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)