Isolation of human brain protein kinase C: evidence for kinase C catalytic fragment modulating G protein-GTPase activity.
Protein kinase C from human brain was isolated and characterized. A protein kinase M like kinase of molecular weight 63 kDa was also partially purified and identified by its immunological properties similar to those of kinase C. The kinase M like kinase activity, devoid of Ca2+ and phospholipids dependency, was also characterized by its inhibition profile by several ligands. Since this kinase phosphorylates a G protein (M.W. 36 kDa) and decreases its GTPase activity which could be restored by alkaline phosphatase, it is concluded that this kinase M like kinase could interact with G protein mediated events of neuronal responses.
Sauvage
C
C
Centre de Recherche De la lande, Rueil-Malmaison, France.
Cash
C D
CD
Maitre
M
M
D000469:Alkaline Phosphatase / Q000378:metabolism; D001921:Brain / Q000201:enzymology*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D007700:Kinetics; D008970:Molecular Weight; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*
D011493:Protein Kinase C; D000469:Alkaline Phosphatase; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins