title: Isolation and characterization of carnitine acetyltransferase from S. cerevisiae.
abstract: Carnitine acetyltransferase was isolated from yeast Saccharomyces cerevisiae with an apparent molecular weight of 400,000. The enzyme contains identical subunits of 65,000 Da. The Km values of the isolated enzyme for acetyl-CoA and for carnitine were 17.7 microM and 180 microM, respectively. Carnitine acetyltransferase is an inducible enzyme, a 15-fold increase in the enzyme activity was found when the cells were grown on glycerol instead of glucose. Carnitine acetyltransferase, similarly to citrate synthase, has a double localization (approx. 80% of the enzyme is mitochondrial), while acetyl-CoA synthetase was found only in the cytosol. In the mitochondria carnitine acetyltransferase is located in the matrix space. The incorporation of 14C into CO2 and in lipids showed a similar ratio, 2.9 and 2.6, when the substrate was [1-14C]acetate and [1-14C]acetylcarnitine, respectively. Based on these results carnitine acetyltransferase can be considered as an enzyme necessary for acetate metabolism by transporting the activated acetyl group from the cytosol into the mitochondrial matrix.
authors.affiliation: University Medical School, Institute of Biochemistry, Pecs, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Kispal
authors.forename: J
authors.initials: J
authors.lastname: Cseko
authors.forename: I
authors.initials: I
authors.lastname: Alkonyi
authors.forename: A
authors.initials: A
authors.lastname: Sandor
mesh_terms: D000108:Acetylcarnitine / Q000378:metabolism; D002332:Carnitine O-Acetyltransferase / Q000302:isolation & purification / Q000378:metabolism*; D015151:Immunoblotting; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D000108:Acetylcarnitine; D002332:Carnitine O-Acetyltransferase