Appraisal of rabies vaccine potency by determination of in vitro, specific interleukin-2 production.
The potency of different rabies vaccines was measured via cell mediated immunity (CMI) assessed by the production of interleukin-2 (IL-2) by CD4+CD8- lymphocytes. IL-2 production by splenocytes from mice immunized with various vaccines was measured following in vitro stimulation with antigens from different rabies and rabies-related strains. IL-2 production was specific, reproducible and correlated with the vaccine protective activity as determined by the pre-exposure NIH test. Our results suggest that measurement of IL-2 production could be used for the appraisal of rabies vaccine potency.
Joffret
M L
ML
Unité de la Rage, Institut Pasteur, Paris, France.
Zanetti
C
C
Morgeaux
S
S
Leclerc
C
C
Sureau
P
P
Perrin
P
P
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D005260:Female; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D009500:Neutralization Tests; D011818:Rabies / Q000517:prevention & control; D011819:Rabies Vaccines / Q000494:pharmacology / Q000592:standards*; D051381:Rats; D013045:Species Specificity; D015164:Vaccines, Inactivated / Q000494:pharmacology / Q000592:standards
D000914:Antibodies, Viral; D007376:Interleukin-2; D011819:Rabies Vaccines; D015164:Vaccines, Inactivated