title: WF-2421, a new aldose reductase inhibitor produced from a fungus, Humicola grisea.
abstract: WF-2421 is a novel aldose reductase inhibitor produced by Humicola grisea. WF-2421 was purified from the culture filtrate by successive ion exchange chromatography and the chemical structure was assigned to be alpha-formamido-5'-(2-formamido-1-hydroxyethyl)- beta,2',6-trihydroxy-3-biphenylpropanoic acid (1) on the basis of spectroscopic evidence. The IC50 value of WF-2421 was 3 x 10(-8) M against partially purified aldose reductase of rabbit lens.
authors.affiliation: Exploratory Research Laboratoires, Fujisawa Pharmaceutical Co., Ltd., Ibaraki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nishikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Tsurumi
authors.forename: H
authors.initials: H
authors.lastname: Murai
authors.forename: K
authors.initials: K
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Okamoto
authors.forename: S
authors.initials: S
authors.lastname: Takase
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Hirota
authors.forename: M
authors.initials: M
authors.lastname: Hashimoto
authors.forename: M
authors.initials: M
authors.lastname: Kohsaka
mesh_terms: D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D001713:Biphenyl Compounds / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D003904:Mitosporic Fungi / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D015394:Molecular Structure; D013055:Spectrophotometry, Infrared
chemical_list: D001713:Biphenyl Compounds; C068085:WF 2421; D000449:Aldehyde Reductase