Corticosteroid effects on lipid lateral diffusion in CEM-C1 and CEM-C7 acute lymphoblastic leukemia cells.
We have examined glucocorticoid effects on CEM-C7 and CEM-C1 subclones of a leukemic human T-cell line using fluorescence photobleaching recovery techniques. Incubation with 10(-5) M triamcinolone acetonide (TA) increased lipid lateral diffusion on steroid-sensitive CEM-C7 cells but had no effect on steroid-resistant CEM-C1 cells. CEM-C7 cells incubated in serum-free medium responded only to TA but, when fetal calf serum was added to the incubation medium, would also respond to 10(-5) M dexamethasone and hydrocortisone. Thus, glucocorticoids can cause increased lipid lateral diffusion in CEM-C7 cells, while having no effect on steroid-resistant CEM-C1 cells.
Bulva
C J
CJ
University of Colorado Health Sciences Center School of Medicine, Denver.
Printen
J
J
Melnykovych
G
G
Cutts
L
L
Barisas
B G
BG
Roess
D A
DA
D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology; D004058:Diffusion; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D007945:Leukemia, Lymphoid / Q000188:drug therapy / Q000378:metabolism*; D050356:Lipid Metabolism; D014222:Triamcinolone Acetonide / Q000494:pharmacology
D005938:Glucocorticoids; D003907:Dexamethasone; D014222:Triamcinolone Acetonide; D006854:Hydrocortisone