A microassay for proteolytic activity.
A quantitative procedure for measuring proteolytic activity, utilizing azoalbumin as substrate, has been developed for use in microtiter plates. An enzyme-linked immunosorbent assay reader is used to measure absorbance. The procedure is sensitive, as well as being both rapid and economical. It is particularly convenient for measuring large numbers of samples, such as fractions from column chromatography.
Plantner
J J
JJ
Lorand V. Johnson Laboratory for Eye Research, Department of Surgery, Case Western Reserve University, Cleveland, Ohio 44106.
D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation; D008666:Metalloendopeptidases / Q000032:analysis; D008832:Microchemistry; D010447:Peptide Hydrolases / Q000032:analysis*; D013053:Spectrophotometry
D010447:Peptide Hydrolases; D008666:Metalloendopeptidases