Characterization of plasmids from Listeria sp.
Out of 139 isolates of Listeria sp. (mainly L. monocytogenes) 107 (78%) contained extrachromosomal DNA. Plasmids from 51 of these isolates were investigated further and covered a range of 8 different-sized molecules as shown by agarose gel electrophoresis. Only one of the 107 isolates contained more than one plasmid. With the exception of one plasmid from an isolate of L. seeligeri, restriction analysis and hybridization experiments showed a high degree of homology between the different plasmids.
Kolstad
J
J
Department of Food Hygiene, Norwegian College of Veterinary Medicine, Oslo.
Rørvik
L M
LM
Granum
P E
PE
D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis*; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D005516:Food Microbiology; D006801:Humans; D008087:Listeria / Q000235:genetics*; D008089:Listeria monocytogenes / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015183:Restriction Mapping
D004269:DNA, Bacterial; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; C038485:GGGCCC-specific type II deoxyribonucleases