On the reaction of wheat lipoxygenase with arachidonic acid and its oxygenated derivatives.
Lipoxygenase was purified from wheat kernels by means of ammonium sulfate precipitation, gel chromatography on Sephadex G-200 and anion exchange chromatography on DEAE-Sephadex A-50. Arachidonic acid was mainly converted by the wheat lipoxygenase to 5D-hydroperoxy-6E,8Z,11Z,14Z-eicosatetraenoic acid (5D8-HPETE) with other HPETE isomers including 8-HPETE being minor products. At higher concentrations of lipoxygenase, multiple oxygenation products such as 5,15-dihydroxyeicosatetraenoic acid (5,15-diHETE) and, to a lower extent, 8,15-diHETE and lipoxin isomers were detected after reduction of the hydroperoxy derivatives primarily formed. Similar results were obtained with 5D8- or 15L8-hydroxyeicosatetraenoic acid as substrate. Moreover, evidence was obtained for leukotriene A4 synthase activity of the wheat lipoxygenase.
Heydeck
D
D
Institute of Biochemistry, Medical School Charité of Humboldt University, Berlin, FRG.
Wiesner
R
R
Kühn
H
H
Schewe
T
T
D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism*; D015289:Leukotrienes / Q000378:metabolism; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D014908:Triticum
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D015289:Leukotrienes; D008041:Linoleic Acids; D016718:Arachidonic Acid; C025348:arachidonic acid 5-hydroperoxide; D019787:Linoleic Acid; D008084:Lipoxygenase; D001094:Arachidonate 5-Lipoxygenase