[The effect of DNAse I on bacterial growth depending on the cultivation conditions and on the physiological characteristics of the inoculate].
The stimulating effect of DNAase 1 on Escherichia coli reproduction has been studied depending on the content of slowly growing cells in the inoculation culture in the phase of delayed growth. Three cell fractions of E. coli have been obtained using the stepwise separation of the population in terms of buoyant density in the phase of delayed growth. In contrast to fractions I and II the fraction III contains cells with delayed growth, permeable to exogenous DNAase 1 and sensitive to the action of this enzyme. The faster growth of bacterial cells has been shown to be caused by the direct ferment action on the slowly growing cells. The autometabolites and autolysates don't take part in this process.
Egorov
C Iu
CIu
Kupriianova
F G
FG
Khaĭbullina
S A
SA
Kolpakov
A I
AI
D001412:Bacillus subtilis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000187:drug effects; D003850:Deoxyribonuclease I / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013268:Stimulation, Chemical
D003470:Culture Media; D004269:DNA, Bacterial; D003850:Deoxyribonuclease I
Vliianie DNKazy I na rost bakteriĭ v zavisimosti ot usloviĭ kul'tivirovaniia i fiziologicheskikh osobennosteĭ inokuliata.