Rapid preparation of lysate for immunoprecipitation using a low protein-binding micro-filter.
A simple method to prepare cell lysate for immunoprecipitation is described. The procedure utilizes filtration of cell lysates by using a low protein-binding filter. This filtration method gave an equivalent result to that of the centrifugation method.
Ayres
M
M
Center for Biotechnology, Baylor College of Medicine, The Woodlands, Texas 77381.
Marshall
H B
HB
Ishikawa
J
J
Takahashi
R
R
D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D002498:Centrifugation; D004591:Electrophoresis, Polyacrylamide Gel; D005374:Filtration; D011233:Precipitin Tests / Q000379:methods*
D000911:Antibodies, Monoclonal