Detection of Listeria monocytogenes by direct colony hybridization on hydrophobic grid-membrane filters by using a chromogen-labeled DNA probe.
A DNA probe specific for Listeria monocytogenes was isolated from a beta-hemolytic recombinant clone of an L. monocytogenes gene bank. It was labeled with horseradish peroxidase and used in a direct colony hybridization method on hydrophobic grid-membrane filters for the detection of the organism. Following color development of the chromogen, a commercial counter (HGMF Interpreter) was able to detect and count the organisms electronically. The method gave a positive reaction with 70 L. monocytogenes strains, while showing a negative reaction with 10 strains of other Listeria spp. and with 20 organisms of other genera.
Peterkin
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Bureau of Microbial Hazards, Health and Welfare Canada, Ottawa, Ontario.
Idziak
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Sharpe
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D002863:Chromogenic Compounds; D015169:Colony Count, Microbial / Q000295:instrumentation; D003124:Colorimetry; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D005069:Evaluation Studies as Topic; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification*; D015336:Molecular Probe Techniques / Q000295:instrumentation; D009693:Nucleic Acid Hybridization; D010957:Plasmids
D002863:Chromogenic Compounds; D015342:DNA Probes; D004269:DNA, Bacterial