Identification and initial utilization of a portion of the smaller plasmid of Anabaena variabilis ATCC 29413 capable of replication in Anabaena sp. strain M-131.
Anabaena variabilis ATCC 29413 contains two cryptic plasmids. Clones of the smaller (41 kb) plasmid, designated pRDS1, in cosmid vectors were used to construct a physical map. A clone bank of pRDS1 constructed by ligating fragments from a XhoII digest of a pRDS1 cosmid clone into a mobilizable plasmid was used to locate an origin of replication of pRDS1. Because we were unable to cure A. variabilis of pRDS1, the clone bank was transferred by conjugation to another strain of Anabaena sp., strain M-131. A 5.3 kb fragment of pRDS1 contained all of the sequences necessary for replication in Anabaena sp. strain M-131 as judged by the ability to rescue the hybrid vector from exconjugants in unchanged from after many generations. Hybrid plasmids derived from pRDS1, one bearing genes for luciferase, were also transferred by conjugation to A. variabilis, where they appeared to recombine with pRDS1.
Murry
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MSU-DOE Plant Research Laboratory, Michigan-State University, East Lansing 48824.
Wolk
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D002872:Chromosome Deletion; D003001:Cloning, Molecular; D003360:Cosmids; D000458:Cyanobacteria / Q000235:genetics*; D004261:DNA Replication; D005822:Genetic Vectors; D010957:Plasmids; D015183:Restriction Mapping; D013045:Species Specificity