[Study of the dependence of gene expression of plague pathogen plasmid 65MD on the host bacterium genome].
The conjugative cointegrate containing Yersinia pestis 65 Md plasmid coding for the production of traction I antigen and mouse toxin has been transferred into Yersinia pseudotuberculosis, Yersinia enterocolitica and Escherichia coli cells. Analysis of the transconjugants obtained has confirmed the connection of the genetical determinants for the mentioned bacterial products with the 65 Md plasmid. The similar level of fra and tox-genes expression has been found in Yersinia cells while fraction I was not produced in Escherichia coli CA cells. The data on the increased synthesis of fraction I at 40 degrees C as compared with the one at 37 degrees C has been obtained. In some cases the production remained at the same level characteristic of the 37 degrees C. The possibility of formation of different Yersinia Fra+ recombinants in the mixed natural epizootic foci and their role in epizootic infections are discussed.
Lebedeva
S A
SA
Kuznetsova
L S
LS
Bichul'
O K
OK
Cherniavskaia
A S
AS
Zarenkov
M I
MI
D003227:Conjugation, Genetic; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005779:Immunodiffusion; D010957:Plasmids; D013696:Temperature; D014162:Transfection; D015008:Yersinia enterocolitica / Q000235:genetics*; D015010:Yersinia pestis / Q000235:genetics*
Izuchenie zavisimosti ékspressii genov plazmidy 65 MD chumnogo mikroba ot genoma bakterii-khoziaina.