Replication factor-A from Saccharomyces cerevisiae is encoded by three essential genes coordinately expressed at S phase.
Replication factor-A (RF-A) is a three-subunit protein complex originally purified from human cells as an essential component for SV40 DNA replication in vitro. We have previously identified a functionally homologous three-subunit protein complex from the yeast Saccharomyces cerevisiae. Here we report the cloning and characterization of the genes encoding RF-A from S. cerevisiae. Each of the three subunits is encoded by a single essential gene. Cells carrying null mutations in any of the three genes arrest as budded and multiply budded cells. All three genes are expressed in a cell-cycle-dependent manner; the mRNA for each subunit peaks at the G1/S-phase boundary. A comparison of protein sequences indicates that the human p34 subunit is 29% identical to the corresponding RFA2 gene product. However, expression of the human protein fails to rescue the rfa2::TRP1 disruption.
Brill
S J
SJ
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724.
Stillman
B
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004261:DNA Replication / Q000235:genetics*; D004271:DNA, Fungal; D004268:DNA-Binding Proteins / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D010641:Phenotype; D010957:Plasmids; D051716:Replication Protein A; D015183:Restriction Mapping; D016196:S Phase; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D016415:Sequence Alignment
D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C497167:RPA1 protein, human; D051716:Replication Protein A