Influence of nonfermented dairy products containing bacterial starter cultures on lactose maldigestion in humans.
The effect of nonfermented dairy products containing yogurt or acidophilus cultures on lactose utilization by lactose-maldigesting humans was investigated. Yogurt and acidophilus milk containing 10(7) or 10(8) of Streptococcus thermophilus and Lactobacillus bulgaricus, or Lactobacillus acidophilus, respectively, were prepared using commercially processed 2% low fat milk. Immediately following inoculation, products were refrigerated. Lactose maldigestion was monitored by measuring breath hydrogen excretion at hourly intervals for 8 h following consumption of 400 ml of each test meal containing approximately 20 g of lactose. The yogurt milk containing 10(8) cfu/ml was shown to contain significant concentrations of microbial beta-galactosidase (EC 3.2.1.23; approximately 3 U/ml), which remained stable for at least 14 d at refrigerator temperatures. Breath hydrogen peaks were delayed and significantly lower (approximately 20 ppm at 5 to 7 h) than control values (approximately 70 ppm at 4 h), and intolerance symptoms were eliminated in all subjects. Yogurt milk containing 10(7) cfu/ml demonstrated intermediate breath hydrogen values and was marginally significantly different from control values. Lactobacillus acidophilus strains with varying resistance to bile and total beta-galactosidase-producing potential were also tested. Only one strain, LA-1, which demonstrated low bile resistance and intermediate beta-galactosidase activity, was capable of significantly decreasing breath hydrogen values when 10(8) cfu/ml of milk was consumed.
Lin
M Y
MY
Department of Food Science and Nutrition, University of Minnesota, St. Paul 55108.
Savaiano
D
D
Harlander
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D000328:Adult; D000818:Animals; D001646:Bile / Q000378:metabolism; D003611:Dairy Products; D004063:Digestion; D004795:Enzyme Stability; D005260:Female; D005516:Food Microbiology; D006801:Humans; D007778:Lactobacillus / Q000378:metabolism*; D007779:Lactobacillus acidophilus / Q000378:metabolism; D007785:Lactose / Q000378:metabolism; D007787:Lactose Intolerance / Q000178:diet therapy*; D008297:Male; D008892:Milk / Q000382:microbiology; D013291:Streptococcus / Q000378:metabolism*; D015014:Yogurt; D001616:beta-Galactosidase / Q000378:metabolism
D001616:beta-Galactosidase; D007785:Lactose