title: Quantitative determination of aflatoxin B1 in chick liver.
abstract: A method for the rapid and quantitative determination of aflatoxin B1 from small quantities of liver, around 1-2 g, is described. The extraction procedure involves acidification to pH 2 of the aqueous liver homogenates, extraction with chloroform: acetone and HPLC-fluorimetric detection after derivatization with trifluoroacetic acid. Quantitative recovery of aflatoxin B1 from chick liver was achieved and detection at levels of 0.2-1 ppb was proved feasible. The aflatoxin B1 concentration in chick liver after oral administration is also shown.
authors.affiliation: Department of Veterinary Medicine, Faculty of Veterinary, Autonomous University of Barcelona, Bellaterra, Spain.
authors.forename: Y
authors.initials: Y
authors.lastname: Espada
authors.forename: R
authors.initials: R
authors.lastname: Guitart
authors.forename: M
authors.initials: M
authors.lastname: Arboix
mesh_terms: D016604:Aflatoxin B1; D000348:Aflatoxins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D002138:Calibration; D002273:Carcinogens / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D008099:Liver / Q000737:chemistry*; D015203:Reproducibility of Results; D014269:Trifluoroacetic Acid
chemical_list: D000348:Aflatoxins; D002273:Carcinogens; D016604:Aflatoxin B1; D014269:Trifluoroacetic Acid