Carbon dioxide-induced hypoxia causes selective loss of rat brain protein kinase C-gamma.
Protein kinase C purified from the brains of rats killed by decapitation contained isozymes -alpha, beta and -gamma, whereas brain from animals killed by CO2-anoxia contained only the -alpha and -beta forms. Immunoblotting experiments established that exposure to CO2 resulted in a selective loss of protein kinase C-gamma protein.
Clark
K J
KJ
School of Biological Sciences, Flinders University, Bedford Park, South Australia.
Isaac
E L
EL
Murray
A W
AW
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002245:Carbon Dioxide / Q000494:pharmacology*; D002458:Cell Fractionation; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D000860:Hypoxia / Q000201:enzymology*; D015151:Immunoblotting; D007527:Isoenzymes / Q000032:analysis / Q000187:drug effects; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats
D007527:Isoenzymes; D002245:Carbon Dioxide; D011493:Protein Kinase C