Striatal A2 receptor regulates apomorphine-induced turning in rats with unilateral dopamine denervation.
The effect of the purine agonist N-ethylcarboxamido-adenosine (NECA) on apomorphine-induced rotation was investigated in rats with unilateral 6-hydroxydopamine lesions of the nigrostriatal pathway. Intrastriatal administration of NECA on the denervated side caused a dose-dependent inhibition of contralateral rotation. This inhibition was prevented by prior intrastriatal injection of theophylline. The adenosine A1 receptor antagonist 8-cyclopentyltheophylline was ineffective at concentrations which block this receptor, but effective in preventing the action of NECA at concentrations which block the adenosine A2 receptor. In the absence of apomorphine, NECA had no effect on behaviour. It is concluded that A2 receptor activation counteracts apomorphine effects in the striatum. Since the A2 receptor may be localized to striatal cholinergic neurones, the possible role of these neurones in purine-induced behaviours is discussed.
Brown
S J
SJ
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, UK.
Gill
R
R
Evenden
J L
JL
Iversen
S D
SD
Richardson
P J
PJ
D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D019830:Adenosine-5'-(N-ethylcarboxamide); D000818:Animals; D001058:Apomorphine / Q000494:pharmacology*; D001923:Brain Chemistry / Q000187:drug effects; D003342:Corpus Striatum / Q000378:metabolism* / Q000502:physiology; D003714:Denervation; D004298:Dopamine / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D006892:Hydroxydopamines / Q000494:pharmacology; D016627:Oxidopamine; D051381:Rats; D011983:Receptors, Purinergic / Q000378:metabolism*; D013239:Stereotyped Behavior / Q000187:drug effects*; D013378:Substantia Nigra / Q000502:physiology; D013563:Sympathectomy, Chemical; D013806:Theophylline / Q000031:analogs & derivatives / Q000494:pharmacology
D006892:Hydroxydopamines; D011983:Receptors, Purinergic; C053907:8-cyclopentyl-1,3-dimethylxanthine; D019830:Adenosine-5'-(N-ethylcarboxamide); D016627:Oxidopamine; D013806:Theophylline; D000241:Adenosine; D001058:Apomorphine; D004298:Dopamine