A computer program for molecular weight determination of DNA fragments (HOWBIG).
The computer program HOWBIG has been developed based on the reciprocal correlation of size to migration distance of DNA in high voltage gradient gel systems (Southern, 1979). For calculation the program automatically chooses three marker bands migrating closest to the calculated band, reducing the error down to approximately 0.5% or less (reciprocal method, local form of calculation). The big advantages of the completely menu-driven program are high accuracy, error detectability, speed and ease of data handling. Management of marker-DNA molecular weights, data and analyses as files included in the menu driven program speeds up every-day lab work.
Grabner
M
M
Institut für Molekularbiologie, Wien, Austria.
Hofbauer
R
R
D004247:DNA / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008838:Microcomputers; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry; D012984:Software
D010446:Peptide Fragments; D004247:DNA