title: Comparison of the new MicroScan Pos MIC Type 6 panel and AMS-Vitek Gram Positive Susceptibility Card (GPS-TA) for detection of high-level aminoglycoside resistance in Enterococcus species.
abstract: We compared the MicroScan Pos MIC Type 6 panel and AMS-Vitek Gram Positive Susceptibility Card (GPS-TA) to agar dilution screen plates for the detection of high-level aminoglycoside resistance in 182 enterococcal isolates. The specificity of the two commercial systems was 100%, with the exception of one susceptible isolate found to be streptomycin resistant by the Vitek system. The MicroScan and Vitek systems had comparable sensitivities for the detection of gentamicin resistance (90 and 95% respectively) and streptomycin resistance (85 and 78%, respectively). These results suggest that screening tests such as agar dilution screen plates, broth dilution, or disk diffusion should continue to be used to detect high-level gentamicin and streptomycin resistance.
authors.affiliation: Department of Microbiology, Mount Sinai Hospital, University of Toronto, Ontario, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Szeto
authors.forename: M
authors.initials: M
authors.lastname: Louie
authors.forename: D E
authors.initials: DE
authors.lastname: Low
authors.forename: M
authors.initials: M
authors.lastname: Patel
authors.forename: A E
authors.initials: AE
authors.lastname: Simor
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D013293:Enterococcus faecalis / Q000187:drug effects / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000295:instrumentation*; D013291:Streptococcus / Q000187:drug effects* / Q000302:isolation & purification; D013307:Streptomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D013307:Streptomycin