Detection of antibodies to p24 and gp41 epitopes of HIV by ELISA using the recombinant core protein (p24) and envelope synthetic protein (gp41).
We have developed a system consisting of two separate ELISA, one designed to detect antibodies to HIV gag gene (p24) and the other to detect antibodies to HIV env gene (gp41). The antigen used in these ELISA was produced as recombinant DNA-derived proteins expressed in E. coli for HIV gag gene (p24) and synthetic peptide for the HIV env gene (gp41). These HIV (env-gag) ELISA, that provide independent determinations of the antibody response to the core and envelope proteins, are highly specific and sensitive. In this work we have demonstrated that determinations of antibodies such as those to p24 and gp41 by HIV (env-gag) ELISA are among the criteria for a confirmation procedure, and sensitivity one (gp41) and/or both these determination should be equal or greater than the sensitivity of W.B. In addition, the procedure should be objective and standardized and the antigen source used should be different from that adopted in the "classical" W.B. and screening test. In view of these considerations, this HIV (env-gag) ELISA could be used as a reliable alternative to W.B. for confirmation of antibody detection.
Filice
G
G
IRCCS Policlinico S. Matteo, Department of Infectious Diseases, University of Pavia.
Soldini
L
L
Orsolini
P
P
Razzini
E
E
Chiapparoli
L
L
Gulminetti
R
R
Cattaneo
E
E
Achilli
G
G
D015492:AIDS Serodiagnosis / Q000379:methods*; D000386:AIDS-Related Complex / Q000276:immunology; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D005189:False Positive Reactions; D015683:Gene Products, gag / Q000276:immunology*; D015483:HIV Antibodies / Q000097:blood*; D016655:HIV Core Protein p24; D015700:HIV Envelope Protein gp41 / Q000276:immunology*; D006801:Humans; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D019966:Substance-Related Disorders / Q000150:complications; D014758:Viral Core Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015683:Gene Products, gag; D015483:HIV Antibodies; D016655:HIV Core Protein p24; D015700:HIV Envelope Protein gp41; D011994:Recombinant Proteins; D014758:Viral Core Proteins