[Use of the Powell method to optimize the composition of HPLC mobile phase--the separation of ephedrine and pseudoephedrine].
The isomers ephedrine and pseudoephedrine are difficult to be separated by HPLC. In this paper, the Powell method was successfully introduced to optimize the composition of mobile phase in HPLC. On a mu Bondapak C18 column (8-10 microns, 3.9 mm x 30 cm), KH2PO4 solution methanol mobile phase system was optimized for separating ephedrine and pseudoephedrine by the Powell method. The optimized composition of the mobile phase was found as: 0.01 mol/L KH2PO4 -MeOH = 94: 6 (V/V). The Powell method was carried out in a process illustrated by tables and figures and 0.95 peak separation function was achieved.
Liang
H X
HX
China Pharmaceutical University, Nanjing.
Yu
R G
RG
Yang
Q H
QH
Ni
K Y
KY
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004809:Ephedrine / Q000302:isolation & purification*; D013237:Stereoisomerism
D004809:Ephedrine