title: pH-induced reorganization of synaptic membrane as revealed by fluorescence anisotropy and energy transfer.
abstract: Two fluorescent probes, 2-(9-anthroyloxy)stearate and 12-(9-anthroyloxy)stearate, were used to investigate the effects of the neutralization of membrane charges on the organization of synaptic plasma membrane. Steady state fluorescence anisotropy measurements showed that a pH decrease provoked a rigidification of the synaptic membrane surface, whereas the bilayer core remained unaffected. The same effect was observed with negatively charged lipid vesicles. The relative distribution of proteins and the probes was estimated by fluorescence energy transfer from protein tryptophans to fluorescent probes: a pH decrease provoked an increase of the energy transfer, which was most pronounced with the surface probe, indicating an average closer packing between proteins and the probes. The modifications induced by a pH decrease were temperature dependent and were most marked at low temperatures. The results suggest that neutralization of the membrane charges provoked a redistribution of both membrane lipids and proteins. These findings are discussed in terms of a heterogeneous distribution of these membrane components.
authors.affiliation: Centre de Neurochimie CNRS, Strasbourg, France.
authors.forename: C
authors.initials: C
authors.lastname: Burgun
authors.forename: A
authors.initials: A
authors.lastname: Waksman
authors.forename: G
authors.initials: G
authors.lastname: Crémel
mesh_terms: D000818:Animals; D001921:Brain / Q000502:physiology; D004735:Energy Transfer; D005454:Fluorescence Polarization / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008081:Liposomes; D051381:Rats; D013570:Synaptic Membranes / Q000502:physiology*; D013816:Thermodynamics
chemical_list: D008081:Liposomes