title: Increment of alpha B-crystallin mRNA in the brain of patient with infantile type Alexander's disease.
abstract: To estimate the expression level of alpha B-crystallin in the brain of infantile type Alexander's disease, the amounts of protein and mRNA of alpha B-crystallin were measured by enzyme immunoassay (EIA) and Northern blot analysis, respectively, in the brain of patient and controls, and in the tissues from glioblastoma and astrocytoma. The alpha B-crystallin protein in the brain of patient was remarkably increased as compared with those of controls. The amount of alpha B-crystallin mRNA of patient was increased about 7-fold compared to the mean value of the control group and higher than that of glioblastoma tissue. These data suggest that increment of alpha B-crystallin mRNA in astrocytes leads to the overexpression of this protein and may be one of the main causes of infantile type Alexander's disease.
authors.affiliation: Department of Pediatrics, Nagoya University School of Medicine, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ochi
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Maehara
authors.forename: A
authors.initials: A
authors.lastname: Nakayama
authors.forename: T
authors.initials: T
authors.lastname: Negoro
authors.forename: H
authors.initials: H
authors.lastname: Shinohara
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Nagatsu
authors.forename: K
authors.initials: K
authors.lastname: Kato
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D001254:Astrocytoma / Q000378:metabolism; D001483:Base Sequence; D015152:Blotting, Northern; D001927:Brain Diseases / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D003459:Crystallins / Q000378:metabolism*; D015342:DNA Probes; D004802:Eosinophilia / Q000378:metabolism*; D005910:Glioma / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism*
chemical_list: D003459:Crystallins; D015342:DNA Probes; D012333:RNA, Messenger