[Modification of the phospholipid composition of the cardiomyocyte sarcolemma in izadrin-induced myocarditis].
A modification of phospholipid composition in rat cardiomyocyte sarcolemma occurring during isadrin myocarditis has been investigated. The fractions of sphingomyelin (SphM), phosphatidylserine (PhS), phosphatidylinositol (PhI), phosphatidylcholine (PhCh), phosphatidylethanolamine (PhEA) have been isolated. The shifts in phospholipid composition during isadrin myocarditis expressing in increase of the amount of choline-containing (PhCh + SphM) and decrease of acid (PhS + PhI) phospholipids have been revealed. During the isadrin myocarditis the incorporation of DL-[3-14C] serine in the fraction of total protein increases and in the fraction of total lipids decreases. The maximum incorporation of the label into total lipids in normal occurs faster than in case of myocarditis. The dynamics of the label distribution between metabolically related PhS, PhEA and PhCh indicates that PhS is the precursor of PhEA, the later being the precursor of PhCh. The label redistribution between PhS, PhEA and PhCh occurs by the sequence of metabolic transformations and by the direct base-exchange reactions. The intensity of the latter has been estimated by the incorporation of radioactive serine into phospholipids of the sarcolemma in vitro. The data obtained demonstrate the inhibitory action of isadrin myocarditis.
Osadchaia
L M
LM
Stefanov
V E
VE
D000818:Animals; D002250:Carbon Radioisotopes; D002384:Catalysis / Q000187:drug effects; D006321:Heart / Q000187:drug effects*; D007545:Isoproterenol / Q000633:toxicity*; D008563:Membrane Lipids / Q000378:metabolism*; D009205:Myocarditis / Q000139:chemically induced* / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D012508:Sarcolemma / Q000187:drug effects* / Q000378:metabolism
D002250:Carbon Radioisotopes; D008563:Membrane Lipids; D010743:Phospholipids; D007545:Isoproterenol
Modifikatsiia fosfolipidnogo sostava sarkolemmy kardiomiotsitov pri izadrinovom miokardite.