Effect of route of exposure and repeated doses on the acute toxicity in mice of the cyanobacterial nicotinic alkaloid anatoxin-a.
Anatoxin-a (ANTX-a) is a potent nicotinic cholinergic alkaloid produced by some toxigenic strains of cyanobacteria. We have examined i.p. and oral LD50 values of two ANTX-a producing Anabaena sp. and synthetic (+)-ANTX-a in outbred Swiss Webster mice. All three appeared equivalent by i.p. injection, but Anabaena flos-aquae NRC-44-1 was approximately twice as potent orally as the other two. Additionally, we were unable to demonstrate development of any biologically significant tolerance to acute ANTX-a exposure using two different repeated dose regimens.
Stevens
D K
DK
Pharmacology/Toxicology Program, College of Pharmacy, Washington State University, Pullman 99164.
Krieger
R I
RI
D000284:Administration, Oral; D000818:Animals; D001427:Bacterial Toxins; D001681:Biological Assay; D000458:Cyanobacteria; D000087522:Cyanobacteria Toxins; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D008297:Male; D008387:Marine Toxins / Q000008:administration & dosage / Q000633:toxicity*; D051379:Mice; D052998:Microcystins; D014326:Tropanes
D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D052998:Microcystins; D014326:Tropanes; C509783:anatoxin a