Avian reovirus S1133 can replicate in mouse L cells: effect of pH and cell attachment status on viral infection.
Previous reports have suggested that avian reovirus S1133 fails to replicate in mouse L cells. In this article, we report that replication does occur under certain culture conditions. The avian reovirus was found to grow in mouse L cells at pH 6.4 and 7.2 but not at pH 8.2. Culture medium with a basic pH directly inhibited viral transcription and genome replication. As a result, viral protein synthesis was also affected. At permissive pH levels, avian reovirus grew better in monolayers than in suspension cultures of L cells because of the influence of cell attachment status on viral macromolecular synthesis. Our results not only show that avian reovirus can replicate in mouse L cells but also help to explain why it did not in previous studies.
Mallo
M
M
Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Santiago de Compostela, Spain.
Martínez-Costas
J
J
Benavente
J
J
D000818:Animals; D001717:Birds; D002448:Cell Adhesion; D002472:Cell Transformation, Viral; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007739:L Cells / Q000502:physiology; D008715:Methionine / Q000378:metabolism; D051379:Mice; D012330:RNA, Double-Stranded / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis / Q000187:drug effects; D012087:Reoviridae / Q000502:physiology*; D014764:Viral Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D014779:Virus Replication
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins; D008715:Methionine