title: Heterologous expression of active manganese peroxidase from Phanerochaete chrysosporium using the baculovirus expression system.
abstract: The cDNA encoding Mn peroxidase isozyme H4 from Phanerochaete chrysosporium was recombined into a baculovirus and heterologously expressed in Sf9 cells. The recombinant Mn peroxidase has the same molecular weight as the native enzyme as determined by SDS-PAGE and cross-reacts with a Mn peroxidase-specific antibody. The recombinant enzyme has a slightly lower pI than the native fungal isozyme H4 indicating some differences in post-translational modification. Phenol red, guaiacol, and vanillylacetone, substrates of the native Mn peroxidase, are oxidized by the recombinant enzyme. All of the activities are dependent on both Mn (II) and H2O2.
authors.affiliation: Department of Molecular and Cell Biology, Pennsylvania State University University Park 16802.
authors.forename: E A
authors.initials: EA
authors.lastname: Pease
authors.forename: S D
authors.initials: SD
authors.lastname: Aust
authors.forename: M
authors.initials: M
authors.lastname: Tien
mesh_terms: D016367:Baculoviridae / Q000235:genetics; D002912:Chrysosporium / Q000201:enzymology; D004271:DNA, Fungal / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D007526:Isoelectric Point; D007527:Isoenzymes / Q000235:genetics; D008970:Molecular Weight; D010544:Peroxidases / Q000096:biosynthesis / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D004271:DNA, Fungal; D007527:Isoenzymes; D011994:Recombinant Proteins; D010544:Peroxidases; C051129:manganese peroxidase