title: RecA-DNA helical filaments in genetic recombination.
abstract: Paul Howard-Flanders et al proposed a molecular model of RecA-mediated recombination reaction six years ago. How does this model stand at present? In answering this question, we focus on two leading ideas of the original model, namely the proposal of the coaxial arrangement of the aligned DNA molecules within helical RecA filaments and the proposal of the ATP independence of the pairing stage of the recombination reaction. Results obtained after the model was proposed are reviewed and compared with these original assumptions and postulates of the model. EM visualization of recombining DNA molecules, studies of the energetics of the RecA-mediated recombination reaction and biochemical analysis of deproteinized joint molecules are fully consistent with a triple-stranded DNA arrangement during the RecA-mediated recombination reaction and demonstrate the ATP independence of the pairing stage of the reaction.
authors.affiliation: Laboratoire d'Analyse Ultrastructurale, Université de Lausanne, Lausanne-Dorigny, Switzerland.
authors.forename: A Z
authors.initials: AZ
authors.lastname: Stasiak
authors.forename: W
authors.initials: W
authors.lastname: Rosselli
authors.forename: A
authors.initials: A
authors.lastname: Stasiak
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D008854:Microscopy, Electron; D008954:Models, Biological; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000378:metabolism; D011938:Rec A Recombinases / Q000378:metabolism*; D011995:Recombination, Genetic
chemical_list: D004277:DNA, Single-Stranded; D009692:Nucleic Acid Heteroduplexes; D000255:Adenosine Triphosphate; D004247:DNA; D011938:Rec A Recombinases