Antibiotic GE2270 a: a novel inhibitor of bacterial protein synthesis. I. Isolation and characterization.
A novel antibiotic, GE2270 A, was isolated from the fermentation broth of a strain of Planobispora rosea. The product was found to inhibit bacterial protein synthesis. Structural characteristics showed similarities between GE2270 A and thiazolyl peptides such as micrococcin which is known to inhibit protein synthesis by acting directly on the ribosome. Despite this similarity GE2270 A showed functional analogy to kirromycin-like antibiotics and pulvomycin, as its molecular target was found to be elongation factor Tu (EF-Tu). GE2270 A is active against Gram-positive microorganism and anaerobes and differs from the other EF-Tu inhibitors in its spectrum of antimicrobial activity.
Selva
E
E
MMDRI, Lepetit Research Center, Gerenzano, Varese.
Beretta
G
G
Montanini
N
N
Saddler
G S
GS
Gastaldo
L
L
Ferrari
P
P
Lorenzetti
R
R
Landini
P
P
Ripamonti
F
F
Goldstein
B P
BP
D000192:Actinomycetales / Q000145:classification / Q000166:cytology / Q000502:physiology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D001421:Bacteria, Anaerobic / Q000187:drug effects* / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis*; D001441:Bacteroides fragilis / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D001936:Moraxella catarrhalis / Q000187:drug effects; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D010456:Peptides, Cyclic / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D011425:Propionibacterium acnes / Q000187:drug effects; D011500:Protein Synthesis Inhibitors / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D013203:Staphylococcal Infections / Q000188:drug therapy; D013844:Thiazoles / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D010455:Peptides; D010456:Peptides, Cyclic; D011500:Protein Synthesis Inhibitors; D013844:Thiazoles; C068122:GE 2270 A