A comparison of the effects of hyperbaric oxygen culture on survival of murine and canine thyroid gland grafts.
Canine thyroid tissue (CTy) was subjected to hyperbaric oxygen culture (HOC) under conditions that affect immunoalteration in murine thyroid tissue (MTy). Survival of autografts and allografts implanted under the kidney capsule was determined after 21 days by 125I uptake and histology. Unlike MTy, autograft CTy subjected to normothermic HOC (95% O2, 5% CO2; 1.76 kg/cm2) for 48 h did not survive (0/8) whereas decrease of culture duration to 24 h resulted in autograft CTy survival (3/3). Under hypothermia (5 degrees C), HOC could be extended to 7 days with autograft CTy survival (3/3 after 4 days and 3/3 after 7 days). Allograft CTy after 24 h of normothermic HOC and 7 days of hypothermic HOC was rejected. Indicators of oxygen free radical injury were determined:catalase activity was comparable in MTy and CTy (means 14.82 and 6.3-10.8 mm/mg protein, respectively) but superoxide dismutase activity was low in CTy (means 0.01-0.29 and 4.75 U/mg protein, respectively). Malondialdehyde content after 48 h of normothermic HOC was higher in CTy than in MTy (means 2215 and 1275 nmol/g, respectively). The results show that CTy is injured by HOC under conditions tolerated by MTy, and that this difference is related to the greater sensitivity of CTy to oxygen free radical injury.
van Gulik
T M
TM
Department of Surgery, University of Amsterdam, The Netherlands.
Boudjema
K
K
Platz
K
K
Hullett
D A
DA
Starling
J R
JR
Southard
J H
JH
Sollinger
H W
HW
Belzer
F O
FO
D000818:Animals; D002374:Catalase / Q000378:metabolism; D004285:Dogs; D005260:Female; D006085:Graft Survival / Q000502:physiology*; D006931:Hyperbaric Oxygenation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000378:metabolism; D013961:Thyroid Gland / Q000033:anatomy & histology / Q000378:metabolism*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase