High-performance liquid chromatographic analysis of 2',3'-dideoxyinosine in biological samples.
A high-performance liquid chromatographic analysis for the anti-AIDS drug 2',3'-dideoxyinosine (ddI) in rat plasma and urine, with a limit of detection of 0.2 microgram/ml and requiring a sample size of 100 microliters is described. Diluted plasma or urine samples were extracted using a C18 solid-phase extraction column. Retention of ddI on more polar solid-phase extraction columns was insufficient for sample clean-up. This method is useful for pharmacokinetic studies of ddI in small rodents.
Wientjes
M G
MG
Division of Urology, Ohio State University, Columbus 43210.
Au
J L
JL
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016049:Didanosine / Q000008:administration & dosage / Q000097:blood* / Q000652:urine; D005260:Female; D007275:Injections, Intravenous; D051381:Rats; D011916:Rats, Inbred F344
D016049:Didanosine