title: Crystallization and preliminary X-ray crystallographic studies of CO-hemoglobin from the North Atlantic salmon (Salmo salar).
abstract: Purification of hemoglobin from North Atlantic salmon (Salmo salar) gave three different types. The CO-complexes of types I and III have been crystallized by the batch method at 4 degrees C from solutions 18% (w/v) in polyethylene glycol 2000, 50 mg/ml in hemoglobin and 0.05 M in phosphate buffer (pH 8.3). Orthorhombic crystals, space group P2(1)2(1)2(1), were obtained for both, with cell dimensions a = 53.9 A, b = 80.4 A, c = 132.4 A, and a = 58.7 A, b = 95.0 A, c = 107.4 A, for types I and III, respectively.
authors.affiliation: Department of Chemistry, University of Tromsø, Norway.
authors.forename: R L
authors.initials: RL
authors.lastname: Larsen
authors.forename: A
authors.initials: A
authors.lastname: Hordvik
authors.forename: E
authors.initials: E
authors.lastname: Hough
authors.forename: K
authors.initials: K
authors.lastname: Jynge
authors.forename: L K
authors.initials: LK
authors.lastname: Hansen
mesh_terms: D000818:Animals; D002245:Carbon Dioxide; D003461:Crystallography; D006454:Hemoglobins / Q000648:ultrastructure*; D012474:Salmon; D014961:X-Ray Diffraction
chemical_list: D006454:Hemoglobins; D002245:Carbon Dioxide