Expression of rat jejunal cystine carrier in Xenopus oocytes.
Functional interactive cystine-lysine carriers have been expressed in Xenopus oocytes following the injection of RNA extracted from rat intestinal mucosa. Lysine-inhibitable cystine uptake was able to be measured 16 h after oocyte injection with RNA. The longer the oocytes were maintained after injection, the more cystine transport capability was induced. Uninjected or water-injected oocytes showed virtually no lysine-inhibitable cystine uptake, and no system developed after the oocytes had been isolated and maintained in vitro. The cystine uptake expressed after RNA injection was selectively inhibited by dibasic amino acids and phenylalanine but not by other amino acids or alpha-methyl-D-glucoside. Expression of the interactive cystine-lysine system was induced only by RNA isolated from intestinal tissue and not by RNA from rat liver. The Km for cystine uptake in RNA-injected oocytes was 0.01 mM and appears identical to the single system found in the RNA source tissue.
McNamara
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Division of Biochemical Development and Molecular Diseases, Children's Hospital of Philadelphia, Philadelphia 19104.
Rea
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Segal
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D000818:Animals; D001692:Biological Transport; D003553:Cystine / Q000378:metabolism*; D005260:Female; D007583:Jejunum / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D008297:Male; D008845:Microinjections; D010063:Ovum / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014981:Xenopus
D012333:RNA, Messenger; D003553:Cystine; D008239:Lysine