Increased osmiophilia of glycosaminoglycan-like structures after fixation with cetylpyridinium chloride in human colonic mucosa.
The effects on tissue ultrastructural appearance of fixation with cetylpyridinium chloride (CPC)-additioned formaldehyde were studied at the ultrastructural level in human colonic mucosa. We found that CPC did not impair tissue morphology and induced a remarkable osmiophilia in glycosamino-glycan-like structures, which did not require further staining.
Malchiodi-Albedi
F
F
Laboratorio di Ultrastrutture, Istituto Superiore di Sanità, Roma, Italia.
Ciaralli
F
F
Giuliani
A
A
D002594:Cetylpyridinium / Q000494:pharmacology*; D003106:Colon / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure; D005404:Fixatives / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000032:analysis*; D006801:Humans; D007413:Intestinal Mucosa / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure; D008854:Microscopy, Electron; D009992:Osmium / Q000032:analysis*
D005404:Fixatives; D006025:Glycosaminoglycans; D009992:Osmium; D002594:Cetylpyridinium