title: Lipoxygenase-mediated secretory effect of canatoxin the toxic protein from Canavalia ensiformis seeds.
abstract: Canatoxin was shown to induce serotonin release from rabbit platelets and rat brain synaptosomes, as well as to release insulin from isolated pancreatic islets. All these effects were dose-dependent and were inhibited by lipoxygenase inhibitors, such as nordihydroguaiaretic acid and esculetin, but not by indomethacin, a cyclooxygenase inhibitor. The data suggest that canatoxin-induced secretory effect results from the activation of the lipoxygenase pathway which would elicit exocytosis. Thus, canatoxin might be a useful tool for the study of biological events that involve lipoxygenase mediation.
authors.affiliation: Dept of Pharmacology, Universidade do Estado do Rio de Janeiro, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Barja-Fidalgo
authors.forename: J A
authors.initials: JA
authors.lastname: Guimarães
authors.forename: C R
authors.initials: CR
authors.lastname: Carlini
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D007887:Fabaceae / Q000032:analysis; D005260:Female; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism; D037102:Lectins / Q000494:pharmacology*; D008084:Lipoxygenase / Q000502:physiology*; D016859:Lipoxygenase Inhibitors; D009637:Masoprocol / Q000494:pharmacology; D037121:Plant Lectins; D010940:Plant Proteins; D010946:Plants, Medicinal; D011817:Rabbits; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects; D012701:Serotonin / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism; D014118:Toxins, Biological / Q000494:pharmacology*; D014468:Umbelliferones / Q000494:pharmacology
chemical_list: D007328:Insulin; D037102:Lectins; D016859:Lipoxygenase Inhibitors; D037121:Plant Lectins; D010940:Plant Proteins; D014118:Toxins, Biological; D014468:Umbelliferones; D012701:Serotonin; D009637:Masoprocol; C032815:canatoxin; D008084:Lipoxygenase; C007628:esculetin; D007213:Indomethacin