Enzyme activities associated with carcinogen metabolism in liver and nonhepatic tissues of rats maintained on high fat and food-restricted diets.
The influence of high fat or food-restricted diets on key enzymes associated with polycyclic aromatic hydrocarbon metabolism was assessed in liver, lung, kidney and stomach of rats. Animals had access ad libitum to the AIN-76A purified diet (control) or were given 65% of the intake of controls for 3 wk. The high fat diet was isoenergetic to the control diet by substituting corn oil for equal energy from carbohydrate and addition of cellulose to obtain equal energy density. Activities of arylhydrocarbon hydroxylase and 7-ethoxycoumarin O-deethylase were significantly increased in lungs of food-restricted rats and decreased by the high fat diet but were not altered in liver. Both diets increased arylhydrocarbon hydroxylase approximately twofold in kidney. Glucose 6-phosphate and 6-phosphogluconate dehydrogenase were lowered in lung, kidney and liver by the high fat diet. Hepatic glutathione S-transferase was increased by high fat feeding. Food restriction decreased activities of arylsulfatase and beta-glucuronidase about 40% in lung. Hepatic arylsulfatase was also decreased about 40% by this treatment. Changes in hydrolase activities in livers and lungs of animals maintained on restricted diets raises in the interesting possibility that net production of glucuronide and sulfate conjugates of carcinogens by the liver and their hydrolysis in lung is altered by food restriction.
Kwei
G Y
GY
Department of Pharmacology and Toxicology, Rutgers University, Piscataway, NJ 08854.
Zaleski
J
J
Thurman
R G
RG
Kauffman
F C
FC
D015660:7-Alkoxycoumarin O-Dealkylase / Q000378:metabolism; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D001192:Arylsulfatases / Q000378:metabolism; D002273:Carcinogens / Q000378:metabolism*; D004041:Dietary Fats / Q000008:administration & dosage* / Q000494:pharmacology; D005508:Food Deprivation; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D008168:Lung / Q000201:enzymology; D008297:Male; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D011083:Polycyclic Compounds / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000201:enzymology
D002273:Carcinogens; D004041:Dietary Fats; D011083:Polycyclic Compounds; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D015660:7-Alkoxycoumarin O-Dealkylase; D001189:Aryl Hydrocarbon Hydroxylases; D014453:Glucuronosyltransferase; D005982:Glutathione Transferase; D001192:Arylsulfatases; D005966:Glucuronidase