Immaturity and aneuploidy in human oocytes after different stimulation protocols.
OBJECTIVE
To study immaturity and aneuploidy in human oocytes after two different stimulation protocols.


METHODS
Retrospective.


METHODS
Outpatient IVF clinic/laboratory.


METHODS
One hundred forty-three patients of whom 65 were stimulated with clomiphene citrate (CC)/human menopausal gonadotropin (hMG) and 78 were stimulated with gonadotropin-releasing hormone agonist (GnRH-a)/hMG. Only patients with at least one oocyte unfertilized were included in this study.


RESULTS
Stimulation with GnRH-a/hMG, as compared with CC/hMG stimulation, resulted in larger numbers of oocytes (P less than 0.00001), a higher fertilization rate (P less than 0.02), and oocyte retrieval at a later average cycle day (P less than 0.000005). Cytogenetic findings of immaturity were observed in 33.9% of unfertilized oocytes after CC/hMG stimulation, compared with only 17.8% after GnRH-a/hMG stimulation (P less than 0.0005). Aneuploidy findings were the same for both groups.


CONCLUSIONS
In GnRH-a/hMG stimulation, oocytes approach the normal day of ovulation more closely. This may allow for better oocyte maturation and higher fertilization and pregnancy rates.
Pieters
M H
MH
Academische Ziekenhuis Maastricht, The Netherlands.
Dumoulin
J C
JC
Engelhart
C M
CM
Bras
M
M
Evers
J L
JL
Geraedts
J P
JP
D000782:Aneuploidy; D002064:Buserelin / Q000008:administration & dosage; D002985:Clinical Protocols; D002996:Clomiphene / Q000008:administration & dosage; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008596:Menotropins / Q000008:administration & dosage; D008597:Menstrual Cycle; D009865:Oocytes / Q000166:cytology* / Q000502:physiology; D010062:Ovulation Induction / Q000379:methods*; D011247:Pregnancy; D012189:Retrospective Studies
D002996:Clomiphene; D008596:Menotropins; D002064:Buserelin