title: Specific targeting of mitomycin C to tumors by anti-gastric cancer monoclonal antibody.
abstract: In the present study, an anti-gastric cancer monoclonal antibody, MGb2, was chosen to prepare antibody-mitomycin C (MMC) conjugate with dextran T-40 as intermediary. Twenty molecules of MMC were introduced into each molecule of antibody while the antigen-binding capacity of the antibody was kept well. The conjugate showed selective cytotoxicity upon human gastric cancer cell line SGC-7901. Radioimmunoimaging and biodistribution studies indicated that after conjugation with MMC via dextran T-40 as intermediary, the tumor localization capacity of the antibody was well retained. When tested in nude mice, inoculated with human gastric carcinoma SGC-7901 in bilateral subrenal capsules, intraperitoneal injection of the conjugate daily for 6 days at a dose of 1 mg/kg gave a tumor inhibitory rate of 68.67%, which was far better than that of free MMC or irrelevant conjugate. No synergetic effect was found in regard to the mixture of MGb2 with MMC.
authors.affiliation: Laboratory of Gastroenterology, Xijing Hospital, Fourth Military Medical University, Xi'an.
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.forename: X Y
authors.initials: XY
authors.lastname: Zhang
authors.forename: X T
authors.initials: XT
authors.lastname: Chen
authors.forename: D M
authors.initials: DM
authors.lastname: Fan
authors.forename: S Y
authors.initials: SY
authors.lastname: Zhan
authors.forename: L J
authors.initials: LJ
authors.lastname: Chen
authors.forename: Y H
authors.initials: YH
authors.lastname: Shu
authors.forename: J L
authors.initials: JL
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D000912:Antibodies, Neoplasm / Q000627:therapeutic use; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D006801:Humans; D000922:Immunotoxins / Q000627:therapeutic use*; D051379:Mice; D008819:Mice, Nude; D016685:Mitomycin; D008937:Mitomycins / Q000627:therapeutic use*; D009368:Neoplasm Transplantation; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology; D013370:Subrenal Capsule Assay; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000970:Antineoplastic Agents; D000922:Immunotoxins; D008937:Mitomycins; D016685:Mitomycin