Development of a rapid latex enhanced turbidimetric assay for retinol binding protein in urine.
We describe a simple, rapid and sensitive homogeneous immunoassay for urinary retinol-binding protein (RBP) using latex particle-enhanced turbidimetric immunoassay. Rabbit anti-human RBP is covalently coupled to 40 nm latex particles and the assay performed on the IL Monarch 2000 centrifugal analyser, with a 20 microL sample volume and the reaction monitored at 340 nm over an 8 min period. The assay range is 0-6 mg/L with a detection limit of 25 micrograms/L. The within and between assay coefficients of variation are less than 1.5% and less than 2.5%, respectively. Comparison with radioimmunoassay for RBP showed good agreement.
Thakkar
H
H
Department of Clinical Biochemistry, London Hospital Medical College, UK.
Cornelius
J
J
Dronfield
D M
DM
Medcalf
E A
EA
Newman
D J
DJ
Price
C P
CP
D007118:Immunoassay / Q000379:methods*; D008863:Microspheres; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation / Q000379:methods*; D011507:Proteinuria / Q000175:diagnosis; D011863:Radioimmunoassay; D012044:Regression Analysis; D012177:Retinol-Binding Proteins / Q000652:urine*; D012680:Sensitivity and Specificity
D012177:Retinol-Binding Proteins