Leukemia inhibitory factor can potentiate murine megakaryocyte production in vitro.
Receptors for murine leukemia inhibitory factor (LIF) were demonstrated on immature and mature murine megakaryocytes. LIF alone had no effects in culture on the survival or proliferation of normal murine megakaryocytes or their precursors. However, combination of LIF with multipotential-colony-stimulating factor (Multi-CSF) (interleukin-3) enhanced the megakaryocyte colony formation able to be stimulated by Multi-CSF; the enhancement involved all types of megakaryocyte colony and resulted in the formation of increased numbers of megakaryocytes. These observations provide a possible basis for the observation that, when LIF is injected in vivo, elevations are observed in megakaryocyte numbers and platelet levels.
Metcalf
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D
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
Hilton
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D
Nicola
N A
NA
D000818:Animals; D001772:Blood Cell Count; D001792:Blood Platelets; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D004357:Drug Synergism; D006131:Growth Inhibitors; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology; D007377:Interleukin-3 / Q000494:pharmacology; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008222:Lymphokines / Q000494:pharmacology*; D008533:Megakaryocytes / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D018121:Receptors, Cytokine; D011971:Receptors, Immunologic / Q000378:metabolism; D053606:Receptors, OSM-LIF
D006131:Growth Inhibitors; D007377:Interleukin-3; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; C507838:Lif protein, mouse; C507841:Lifr protein, mouse; D008222:Lymphokines; D018121:Receptors, Cytokine; D011971:Receptors, Immunologic; D053606:Receptors, OSM-LIF