Germ-line origin of functional idiotypic interactions: identification of two idiotypically connected, natural antibodies that are encoded by germ-line gene elements.
Two monoclonal, natural IgM antibodies derived from normal BALB/c mice were selected on the basis of being idiotypically complementary and functionally connected. Nucleotide sequence analysis of their respective heavy and light chain V regions showed that both of the clones expressed VH, D, JH, VL and JL gene segments of germ-line origin. Furthermore, none of the clones displayed N-sequence additions. These data suggest a germ-line origin of a functional idiotypic network and confirm a minimized contribution of somatic diversification through template-independent addition of N-nucleotides in neonatal B cell repertoires.
Carlsson
L
L
Unit for Applied Cell and Molecular Biology, University of Umeå, Sweden.
Andersson
A
A
Holmberg
D
D
D000818:Animals; D001483:Base Sequence; D005803:Genes, Immunoglobulin; D006825:Hybridomas / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007130:Immunoglobulin Idiotypes / Q000235:genetics*; D007133:Immunoglobulin Joining Region / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007075:Immunoglobulin M / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D015347:RNA Probes / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007133:Immunoglobulin Joining Region; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D015347:RNA Probes