title: Improved site-directed mutagenesis method using PCR.
authors.affiliation: Department of Biophysical Chemistry, Netherlands Institute for Dairy Research (NIZO), Ede.
authors.forename: O P
authors.initials: OP
authors.lastname: Kuipers
authors.forename: H J
authors.initials: HJ
authors.lastname: Boot
authors.forename: W M
authors.initials: WM
authors.lastname: de Vos
mesh_terms: D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D009838:Oligodeoxyribonucleotides / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides