title: Collaborative study of the International Office of Cocoa, Chocolate and Sugar Confectionery on Salmonella detection from cocoa and chocolate processing environmental samples.
abstract: A comparative collaborative study was performed in 13 laboratories to evaluate the use of motility enrichment on Modified Semisolid Rappaport-Vassiliadis medium for rapid Salmonella detection from food-processing environmental samples. Artificially contaminated chocolate scrapings and naturally contaminated cocoa bean dust samples were used in the study. Pre-enrichment was performed in buffered peptone water with added casein and malachite green oxalate. Motility enrichment was compared with a conventional cultural procedure using Rappaport-Vassiliadis broth and selenite cystine broth as selective enrichment. The productivity of motility enrichment was 93.5% compared to a productivity of the cultural procedure of 92%. Statistical analysis showed that there was no significant difference between the two procedures. Modified Semisolid Rappaport-Vassiliadis medium is a sensitive and simple diagnostic tool for the microbiological safety evaluation of food-processing environments.
authors.affiliation: Jacobs Suchard, Herentals, Belgium.
authors.forename: J M
authors.initials: JM
authors.lastname: De Smedt
authors.forename: S
authors.initials: S
authors.lastname: Chartron
authors.forename: J L
authors.initials: JL
authors.lastname: Cordier
authors.forename: E
authors.initials: E
authors.lastname: Graff
authors.forename: H
authors.initials: H
authors.lastname: Hoekstra
authors.forename: J P
authors.initials: JP
authors.lastname: Lecoupeau
authors.forename: M
authors.initials: M
authors.lastname: Lindblom
authors.forename: J
authors.initials: J
authors.lastname: Milas
authors.forename: R M
authors.initials: RM
authors.lastname: Morgan
authors.forename: R
authors.initials: R
authors.lastname: Nowacki
mesh_terms: D002099:Cacao / Q000382:microbiology*; D015169:Colony Count, Microbial; D003470:Culture Media; D005511:Food Handling; D005516:Food Microbiology; D012475:Salmonella / Q000302:isolation & purification*
chemical_list: D003470:Culture Media