[The role of malate in regulating the rate of mitochondrial respiration in vitro].
Depletion of endogenous malate by preincubation of mitochondria at 30 degrees C in substrate-free media sharply decreases the rate of citrate oxidation and inhibits mitochondrial respiration in the presence of pyruvate and alpha-ketoglutarate. Addition of catalytic amounts of endogenous malate and its production via succinate oxidation promote rapid oxidation of citrate and pyruvate in the mitochondria and abolishes the lag period with alpha-ketoglutarate Malate increases the rate of membrane potential generation after addition of citrate, pyruvate or alpha-ketoglutarate to mitochondrial suspensions. Studies with controlled malate concentrations revealed that the changes in malate concentrations observed in the mitochondria in the presence of gluconeogenesis-inducing hormones may be due to the influence of these hormones on mitochondrial oxidation.
Vovyleva-Guarriero
V B
VB
Wehbie
R S
RS
Muscatello
U
U
Lardi
G A
GA
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000502:physiology; D002951:Citrates / Q000378:metabolism; D019343:Citric Acid; D007656:Ketoglutaric Acids / Q000378:metabolism; D008293:Malates / Q000378:metabolism*; D008297:Male; D008564:Membrane Potentials; D008930:Mitochondria, Liver / Q000378:metabolism* / Q000502:physiology; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D051381:Rats; D011919:Rats, Inbred Strains; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid
D002951:Citrates; D007656:Ketoglutaric Acids; D008293:Malates; D011773:Pyruvates; D013386:Succinates; D019343:Citric Acid; D019289:Pyruvic Acid; D019802:Succinic Acid
Rol' malata v reguliatsii skorosti mitokhondrial'nogo dykhaniia in vitro.