title: Subcellular distribution of enzymes involved in the biosynthesis of cyanelle murein in the protist Cyanophora paradoxa.
abstract: Cyanelle containing organisms, notably Cyanophora paradoxa, the best studied among them, are unique with respect to the occurrence of peptidoglycan (murein) within an eukaryotic cell. Enzyme activities involved in the biosynthesis of UDP-N-acetyl-muramylpentapeptide could be localized within the cyanelle compartment. Some of the enzymes performing later steps of murein biosynthesis were detected in the postcyanelle supernatant rather than in the cyanelle lysate. This is taken to reflect a 'periplasmic' location of these enzymes that are partially liberated upon rupture of the cyanelle outer membrane.
authors.affiliation: Institut für Allgemeine Biochemie der Universität Wien, Vienna, Austria.
authors.forename: B
authors.initials: B
authors.lastname: Plaimauer
authors.forename: B
authors.initials: B
authors.lastname: Pfanzagl
authors.forename: J
authors.initials: J
authors.lastname: Berenguer
authors.forename: M A
authors.initials: MA
authors.lastname: de Pedro
authors.forename: W
authors.initials: W
authors.lastname: Löffelhardt
mesh_terms: D002268:Carboxypeptidases / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D056890:Eukaryota / Q000201:enzymology* / Q000648:ultrastructure; D015388:Organelles / Q000201:enzymology*; D010457:Peptidoglycan / Q000096:biosynthesis*; D014538:Uridine Diphosphate N-Acetylmuramic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis
chemical_list: D010457:Peptidoglycan; D014538:Uridine Diphosphate N-Acetylmuramic Acid; C002225:UDP-N-acetylmuramic acid pentapeptide; D002268:Carboxypeptidases; D010450:Endopeptidases