title: Decreased DNA polymerase sensitivity to 1-beta-D-arabinofuranosylcytosine 5'-triphosphate in P388 murine leukemic cells resistant to vincristine.
abstract: P388 murine leukemic cells 28.6-fold resistant to vincristine were cross-resistant to doxorubicin and etoposide. Although the intracellular ara-CTP in P388 murine leukemic cells resistant to vincristine (P388/VCR) was significantly higher than that in the parent cells, the cytotoxic effect of ara-C was not significantly different between the parent and resistant cells. Therefore, we investigated the DNA synthesis in P388/VCR and its parent cell line using the permeable cell system. The DNA synthesis in P388/VCR cells was less sensitive to ara-CTP and dTTP than that in P388 parent cells. These results suggested that the characteristics of DNA polymerase in P388/VCR cells might change when the cells developed multiple drug resistance.
authors.affiliation: Department of Pediatrics, Mie University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Higashigawa
authors.forename: M
authors.initials: M
authors.lastname: Ido
authors.forename: Y
authors.initials: Y
authors.lastname: Nagao
authors.forename: H
authors.initials: H
authors.lastname: Kuwabara
authors.forename: H
authors.initials: H
authors.lastname: Hori
authors.forename: T
authors.initials: T
authors.lastname: Ohkubo
authors.forename: H
authors.initials: H
authors.lastname: Kawasaki
authors.forename: M
authors.initials: M
authors.lastname: Sakurai
mesh_terms: D000818:Animals; D001085:Arabinofuranosylcytosine Triphosphate / Q000378:metabolism*; D015153:Blotting, Western; D003561:Cytarabine / Q000633:toxicity; D004259:DNA-Directed DNA Polymerase / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology; D004351:Drug Resistance; D005047:Etoposide / Q000494:pharmacology; D007424:Intracellular Fluid / Q000378:metabolism; D007941:Leukemia P388 / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D019384:Nucleic Acid Synthesis Inhibitors; D013942:Thymine Nucleotides / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014750:Vincristine / Q000494:pharmacology*
chemical_list: D019384:Nucleic Acid Synthesis Inhibitors; D013942:Thymine Nucleotides; D003561:Cytarabine; D001085:Arabinofuranosylcytosine Triphosphate; D014750:Vincristine; D005047:Etoposide; D004317:Doxorubicin; D004259:DNA-Directed DNA Polymerase; C024157:thymidine 5'-triphosphate