In Lactococcus lactis subsp. cremoris SK110 protein, instead of lipoteichoic acid, reacts with the group-N-specific antiserum.
The reaction between cell-surface components, isolated from two Lactococcus lactis subsp. cremoris strains, with their Group-specific antiserum were studied. No reaction between purified lipoteichoic acid and the antiserum was observed. Both strains, however, did belong to the lactococci (Group-N streptococci), as was demonstrated by the positive reaction between the antiserum and an acid- (Lancefield) or alkaline-extract. Experiments with proteolytic enzymes demonstrated the involvement of protein in the antigenic material in the latter reaction.
Sijtsma
L
L
Biotechnology Centre, University of Amsterdam, The Netherlands.
Hellingwerf
K J
KJ
D000942:Antigens, Bacterial / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D015153:Blotting, Western; D007106:Immune Sera / Q000378:metabolism*; D013294:Lactococcus lactis / Q000276:immunology* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D011233:Precipitin Tests; D013682:Teichoic Acids / Q000378:metabolism
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007106:Immune Sera; D008070:Lipopolysaccharides; D013682:Teichoic Acids; C009900:lipoteichoic acid