Detection of G proteins in bovine brain clathrin coated vesicles with common alpha and beta subunits antibodies.
Clathrin coated vesicles (CV) from bovine brain were analyzed via Western blots for the presence of the alpha and beta subunits of guanine nucleotide regulatory proteins. The results with the common alpha antibody GA/1 revealed the presence of apparently undissociated G-protein subunits migrating at approximately 80 kDa. The predominant band was of about 39-41 kDa, with minor labeling observed in the 41-52 kDa range. Western blot analysis for G beta subunit(s) revealed the presence of a single band of about 35-36 kDa.
Silva
W I
WI
Department of Pharmacology, Universidad Central del Caribe, School of Medicine, Bayamón, Puerto Rico 00621-6032.
D000818:Animals; D000906:Antibodies; D001923:Brain Chemistry; D002417:Cattle; D003034:Coated Pits, Cell-Membrane / Q000737:chemistry*; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000276:immunology; D015151:Immunoblotting
D000906:Antibodies; D019204:GTP-Binding Proteins