title: Measurement of prostaglandin G/H synthase and lipoxygenase activity in the stomach wall by HPLC.
abstract: A method for the determination of prostaglandin G/H synthase and lipoxygenase activities in tissues was developed and employed with rat gastric mucosa samples. Tissues and microsomes were incubated in a buffer containing nonionic detergent and 1.32 mM arachidonic acid for 10 min. Following extraction with ethyl acetate, the oxidation products of arachidonic acid were derivatized with panacyl bromide. A reversed-phase column and a quaternary mobile phase were used to separate and quantitate the panacyl bromide esters of prostaglandin E2 and leukotriene C4/D4. Prostaglandin G/H synthase and lipoxygenase activities were determined in gastric mucosa and were 371 +/- 66 and 173 +/- pg/mg/min, respectively.
authors.affiliation: Long Island Jewish Medical Center.
authors.forename: T A
authors.initials: TA
authors.lastname: Stein
authors.forename: B
authors.initials: B
authors.lastname: Bailey
authors.forename: L J
authors.initials: LJ
authors.lastname: Auguste
authors.forename: L
authors.initials: L
authors.lastname: Wise
mesh_terms: D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005753:Gastric Mucosa / Q000201:enzymology*; D015289:Leukotrienes / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D051381:Rats
chemical_list: D001095:Arachidonic Acids; D015289:Leukotrienes; D016718:Arachidonic Acid; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases