title: Cytotoxicity of T-2 toxin and its metabolites determined with the neutral red cell viability assay.
abstract: The neutral red (NR) cell viability assay was used with various cell types of human origin to quantitate the potency of T-2 mycotoxin and its metabolites. The human melanoma SK-Mel/27 cell line was the most sensitive, with a midpoint cytotoxicity value of 2.8 ng of T-2 per ml. With the human hepatoma cell line, HepG2, the sequence of potency for a series of mycotoxins was T-2 greater than HT-2 greater than T-2 triol greater than T-2 tetraol.
authors.affiliation: Laboratory Animal Research Center, Rockefeller University, New York, New York 10021.
authors.forename: H
authors.initials: H
authors.lastname: Babich
authors.forename: E
authors.initials: E
authors.lastname: Borenfreund
mesh_terms: D000826:Animal Testing Alternatives; D002460:Cell Line; D002470:Cell Survival; D006801:Humans; D008545:Melanoma; D009499:Neutral Red; D013605:T-2 Toxin / Q000378:metabolism / Q000633:toxicity*; D014407:Tumor Cells, Cultured
chemical_list: D009499:Neutral Red; D013605:T-2 Toxin