Development and application of a nonradioactive phosphorescent autoradiograph marker.
A simple and rapid method for permanently marking autoradiographs is described. This procedure is based on the phosphorescence of light-activated zinc sulfide and the subsequent exposure of x-ray films by this light emission. A lacquer-based carrier allows the zinc sulfide to remain in suspension and permits permanent marking onto diverse laboratory substrates such as x-ray films, paper, plastic wraps and nitrocellulose- and nylon-based membranes. An analogous wax-based carrier allows marking onto paper and dried acrylamide gels, which is useful for processing large numbers of radioactive DNA sequencing gels on a high-throughput scale. These inexpensive and nonhazardous markers will be useful in protocols that use x-ray films, regardless of whether radioactive or nonradioactive detection systems are used.
Seto
D
D
Division of Biology, California Inst. of Technology, Pasadena 91125.
Rohrabacher
C
C
D001345:Autoradiography / Q000379:methods*; D008163:Luminescent Measurements; D013440:Sulfides; D014962:X-Ray Film; D015032:Zinc; D017967:Zinc Compounds
D013440:Sulfides; D017967:Zinc Compounds; D015032:Zinc; C031238:zinc sulfide