title: Proteoglycan synthesis and content in articular cartilage and cartilage repair tissue in horses.
abstract: Hexosamine concentration, DNA concentration, and [35S]sulfate incorporation for articular cartilage obtained from various sites in the metacarpophalangeal and carpal joints of horses were measured. The same measurements were made on the repair tissue filling full-thickness articular defects in the intermediate carpal bone and on cartilage surrounding partial-thickness defects 6 weeks after the defects were created arthroscopically. Cellularity (measured as DNA concentration), proteoglycan content (measured as hexosamine concentration), and proteoglycan synthesis (measured as [35S]sulfate incorporation) varied according to the site sampled. Cartilage from the transverse ridge of the head of the third metacarpal bone and the radial facet of the third carpal bone had the lowest hexosamine concentration, whereas rate of proteoglycan synthesis was lowest in cartilage from the transverse ridge of the head of the third metacarpal bone and the distal articular surface of the radial carpal bone. Repair tissue filling a full-thickness cartilage defect at 6 weeks was highly cellular. It was low in proteoglycan content, but was actively synthesizing these macromolecules. In contrast, the cartilage surrounding a partial-thickness defect was unchanged 6 weeks after the original defect was made.
authors.affiliation: Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, New Bolton Center, Kennett Square 19348.
authors.forename: D W
authors.initials: DW
authors.lastname: Richardson
authors.forename: C C
authors.initials: CC
authors.lastname: Clark
mesh_terms: D000818:Animals; D002356:Cartilage / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D002358:Cartilage, Articular / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D002455:Cell Division; D004247:DNA / Q000032:analysis; D006595:Hexosamines / Q000032:analysis / Q000096:biosynthesis; D006736:Horses / Q000378:metabolism*; D011509:Proteoglycans / Q000096:biosynthesis*; D013431:Sulfates / Q000378:metabolism
chemical_list: D006595:Hexosamines; D011509:Proteoglycans; D013431:Sulfates; D004247:DNA