Chemopreventive efficacy of betel leaf extract and its constituents on 7,12-dimethylbenz(a)anthracene induced carcinogenesis and their effect on drug detoxification system in mouse skin.
Effects of topically applied betel leaf extract (BLE) and its constituents. beta-carotene, alpha-tocopherol, eugenol and hydroxychavicol on 7,12-dimethylbenz(a)anthracene (DMBA) induced skin tumors were evaluated in two strains of mice. BLE, beta-carotene and alpha-tocopherol, significantly inhibited the tumor formation by 83, 86, 86% in Swiss mice and 92, 94 and 89% in male Swiss bare mice respectively. Hydroxychavicol showed 90% inhibition in Swiss bare mice at 24 weeks of treatment. Eugenol showed minimal protection in both strains of mice. The mean latency period and survivors in BLE, beta-carotene, alpha-tocopherol and hydroxychavicol treated groups were remarkably high as compared to DMBA alone treated group. Intraperitoneal injection of betal leaf constituents showed a significant effect on both glutathione and glutathione S-transferase levels in the Swiss mouse skin.
Azuine
M A
MA
Carcinogenesis Division & Bio-organic Unit, Cancer Research Institute, Bombay, India.
Amonkar
A J
AJ
Bhide
S V
SV
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic; D001114:Areca / Q000032:analysis*; D005260:Female; D008658:Inactivation, Metabolic; D008297:Male; D051379:Mice; D010946:Plants, Medicinal; D012878:Skin Neoplasms / Q000139:chemically induced / Q000517:prevention & control*
D000972:Antineoplastic Agents, Phytogenic; D015127:9,10-Dimethyl-1,2-benzanthracene