title: Influence of temperature and gas atmosphere on in-vitro fertilization and embryo development in domestic cats.
abstract: The influence of culture temperature and gas atmosphere on in-vitro fertilization and embryo development was examined in the domestic cat. In Exp. 1, eggs were fertilized and cultured in 5% CO2 in air at 37, 38 or 39 degrees C. Experiment 2 evaluated the effects of 5% CO2 in air; 5% CO2, 5% O2 and 90% N2; and 10% CO2 in air. Fertilization (cleavage) and development to the morula/blastocyst stage were not influenced (P greater than 0.05) by variations in temperature and gas composition. Despite changing these culture conditions, egg cleavage averaged approximately 75% and greater than 80% of the 2-cell embryos proceeded to morulae in vitro. However, the partial in-vitro morula-to-blastocyst developmental block normally observed in this species was not removed.
authors.affiliation: National Zoological Park, Smithsonian Institution, Washington, DC 20008.
authors.forename: L A
authors.initials: LA
authors.lastname: Johnston
authors.forename: A M
authors.initials: AM
authors.lastname: Donoghue
authors.forename: S J
authors.initials: SJ
authors.lastname: O'Brien
authors.forename: D E
authors.initials: DE
authors.lastname: Wildt
mesh_terms: D000818:Animals; D002245:Carbon Dioxide; D002415:Cats; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005740:Gases; D009584:Nitrogen; D010100:Oxygen; D013696:Temperature
chemical_list: D005740:Gases; D002245:Carbon Dioxide; D009584:Nitrogen; D010100:Oxygen