A simple method for introducing a thiol group at the 5'-end of synthetic oligonucleotides.
Kumar
A
A
National Institute of Immunology, New Delhi, India.
Advani
S
S
Dawar
H
H
Talwar
G P
GP
D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D007460:Iodoacetamide / Q000031:analogs & derivatives / Q000138:chemical synthesis; D008301:Maleimides / Q000737:chemistry; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis*; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013439:Sulfhydryl Reagents / Q000737:chemistry
D008301:Maleimides; D009841:Oligonucleotides; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; C003944:N-(3-pyrene)maleimide; D007460:Iodoacetamide