Gamma-interferon effects on human fibroblasts from Tenon's capsule.
Gamma-interferon (G-IFN) exhibits antiproliferative effects, inhibits collagen synthesis by fibroblasts, and may help to regulate the turnover of fibrous tissue. Fibroblasts from Tenon's capsule are cellular components that contribute to unsuccessful glaucoma filtration surgery. Therefore, the authors studied the effects of G-IFN on growth, wound closure, collagen synthesis, and extracellular matrix in cultured human Tenon's capsule fibroblasts (HTCF). HTCF incubated with G-IFN at doses of 1 to 1000 U/ml showed cellular heterogeneity, with some cells showing morphologic changes characteristic of senescence, and a dose-dependent inhibition of wound closure. At 1000 U/ml, G-IFN reduced collagen synthesis by 57%, reduced immunofluorescent staining of collagen type I and fibronectin network, and altered the organization of actin microfilaments into large cable-like structures. Cell proliferation and viability were not affected at any concentration of G-IFN. These results suggest that G-IFN may be useful to modulate wound healing after filtration surgery.
Latina
M A
MA
Wellman Laboratories, Massachusetts General Hospital, Boston 02114.
Belmonte
S J
SJ
Park
C
C
Crean
E
E
D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005123:Eye / Q000166:cytology*; D005205:Fascia / Q000166:cytology*; D005347:Fibroblasts / Q000187:drug effects*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D011994:Recombinant Proteins; D013285:Strabismus / Q000601:surgery; D014945:Wound Healing / Q000187:drug effects
D003598:Cytoskeletal Proteins; D011994:Recombinant Proteins; D007371:Interferon-gamma; D003094:Collagen