Use of an A-T-rich DNA clone for identification and detection of Peronosclerospora sorghi.
A recombinant plasmid, pMLY12-1, screened from a Peronosclerospora sorghi library hybridizes only to DNA of P. sorghi, or to DNA from leaves infected with P. sorghi, not to DNA of P. sorghi Thailand isolate, P. philippinensis, P. sacchari, or P. maydis. The terminal sequences of the 1.3-kb insert, which appears to contain mitochondrial DNA, are 85% A and T. No polymorphisms were detected when the probe was hybridized to Southern blots containing DNA from P. sorghi pathotype 1, pathotype 3, or a Botswana isolate digested with any of the eight restriction endonucleases tested. The banding patterns were the same whether DNA was extracted directly from the fungus or from infected leaves.
Yao
C L
CL
Department of Plant Pathology, Texas A&M University, College Station 77843.
Magill
C W
CW
Frederiksen
R A
RA
D001482:Base Composition; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D004274:DNA, Recombinant; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009868:Oomycetes / Q000235:genetics / Q000302:isolation & purification*
D015342:DNA Probes; D004271:DNA, Fungal; D004274:DNA, Recombinant