Interleukin-3 enhances cytokine production by LPS-stimulated macrophages.
In addition to its hematopoietic activities, interleukin-3 (IL-3) can modulate macrophage functions. We have studied the production of interleukin-1 (IL-1), interleukin-6 (IL-6) and tumor necrosis factor (TNF) by mouse peritoneal macrophages triggered by lipopolysaccharide (LPS) in the presence or absence of IL-3. Interleukin-3 at the concentration used (i.e., 100 U/ml) did not induce the production of any cytokines, whereas it enhanced significantly the secretion of IL-1, IL-6 and TNF by LPS-stimulated macrophages. The synergistic activity of IL-3 was observed over a wide range of Escherichia coli or Salmonella enteritidis LPS concentrations. No additive effect was noticed between IL-3 and granulocyte/macrophage colony-stimulating factor (GM-CSF), another factor able to enhance LPS-induced IL-1 production. Thus, IL-3 can potentiate the inflammatory response induced by endotoxin from Gram-negative bacteria through a potentiation of cytokine production.
Cohen
L
L
Unité d'Immuno-Allergie, Institut Pasteur, Paris, France.
David
B
B
Cavaillon
J M
JM
D000818:Animals; D016207:Cytokines / Q000096:biosynthesis* / Q000378:metabolism; D004347:Drug Interactions; D004731:Endotoxins / Q000494:pharmacology*; D004926:Escherichia coli; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000494:pharmacology; D012477:Salmonella enteritidis; D013268:Stimulation, Chemical
D016207:Cytokines; D004731:Endotoxins; D007377:Interleukin-3; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor