[High functional activity of mitochondrial creatine kinase of the myocardium before ischemia is tied with the worst restoration of contractility after reperfusion].
Isolated Langendorff-perfused guinea pig hearts were arrested with a cardioplegic solution containing 10 mM phosphocreatine + 15 mM glutamate (PG group) or not containing them (control group). Total normothermic ischemia lasted 45 min followed by 30 min reperfusion. Mitochondrial respiration in the absence and presence of different concentrations of ADP and creatine was studied in biopsy samples after saponin treatment. The samples were taken before and after ischemia as well as after the reperfusion period. A slightly better relative recovery of developed pressure (RRDP) in PG group was associated with higher mitochondrial acceptor control ratio after reperfusion. When results in both groups were taken together, marked negative correlations between the preischemic mitochondrial indices (particularly, those related to creatine kinase activity) and RRDP were revealed. Relative changes in these indices after ischemia demonstrated tight positive correlations with RRDP. Thus, the hearts having higher functional activity of mitochondrial creatine kinase are more sensitive to ischemia, other conditions being equal.
Veksler
V I
VI
Khatkevich
A N
AN
Kapel'ko
V I
VI
D000818:Animals; D001706:Biopsy; D003327:Coronary Disease / Q000201:enzymology*; D003402:Creatine Kinase / Q000032:analysis*; D006168:Guinea Pigs; D006324:Heart Arrest, Induced; D008929:Mitochondria, Heart / Q000201:enzymology*; D009200:Myocardial Contraction; D015425:Myocardial Reperfusion
D003402:Creatine Kinase
Vysokaia funktsional'naia aktivnost' mitochondrial'noĭ kreatinkinazy miokarda do ishemii sochetaetsia s khudshim vosstanovleniem sokratimosti posle reperfuzii.