Differential reactivity of V beta 9 T cells to minor lymphocyte stimulating antigen in vitro and in vivo.
The monoclonal antibody (mAb) MR10-2 is specific for mouse T cells expressing the V beta 9 chain of T cell antigen receptor. This mAb reacts with 2% of peripheral T cells in a number of different strains of mice, but in mice expressing the minor lymphocyte stimulating (Mls)-1a antigen, V beta 9+ T cells recognized by MR10-2 are deleted. However, we failed to demonstrate the Mls-1 a antigen-specific response of naive, non-activated V beta 9 T cells in vitro. On the other hand, T cells activated initially with anti-V beta 9 (MR10-2) mAb in vitro are capable of responding to the Mls-1a antigen. These results demonstrate that Mls-1a reactivity of V beta 9 T cells depends on the activation state of the T cells, suggesting a differential activation requirement between naive, non-activated T cells and previously activated T cells.
Utsunomiya
Y
Y
Department of Medicine, Washington University School of Medicine, St. Louis. MO 63110.
Kosaka
H
H
Kanagawa
O
O
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D016747:Minor Lymphocyte Stimulatory Antigens; D011948:Receptors, Antigen, T-Cell / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D016747:Minor Lymphocyte Stimulatory Antigens; D011948:Receptors, Antigen, T-Cell