Quantitative assessment of the genotoxicity of fecapentaenes.
Fecapentaenes are a group of fecal mutagens of microbial origin isolated from human stools. Fecapentaene-12 (F-12) and fecapentaene-14 (F-14), differing only in two carbon atoms in the side chain, are glyceryl ethers with a highly reactive chromophoric aliphatic side chain incorporating a conjugated pentaene moiety. Although these compounds are known for their genotoxicity, no test systems have been developed to precisely assess their relative genotoxicity. In this study F-12 and F-14 were assayed for their genotoxicity using the SOS Chromotest in which the induction of beta-galactosidase in E. coli PQ37 was used as a quantitative measure of biological activity. The activity obtained with F-12 and F-14 was compared with that of 4-nitroquinoline oxide (4-NQO) as the reference standard of a direct acting mutagen. While F-14 was almost as active as 4-NQO, F-12 was only about 25% as active as F-14, the higher analog.
Nair
P P
PP
Lipid Nutrition Laboratory, Beltsville Human Nutrition Research Center, U.S. Department of Agriculture, MD 20705.
Shami
S
S
Sainz
E
E
Judd
J T
JT
Taylor
P R
PR
Schatzkin
A
A
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D004790:Enzyme Induction; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D006801:Humans; D009152:Mutagenicity Tests; D009153:Mutagens; D011090:Polyenes / Q000633:toxicity*; D013014:SOS Response, Genetics / Q000187:drug effects; D013056:Spectrophotometry, Ultraviolet; D001616:beta-Galactosidase / Q000096:biosynthesis
D009153:Mutagens; D011090:Polyenes; D015112:4-Nitroquinoline-1-oxide; C037358:1-(1-glycero)tetradeca-1,3,5,7,9-pentaene; C037357:1-(1-glycero)dodeca-1,3,5,7,9-pentaene; D001616:beta-Galactosidase