title: [Hybrid human lymphokine genes. I. Design of recombinant plasmids coding hybrids of immune interferon and human tumor necrosis factor].
abstract: Recombinant plasmids coding for hybrid proteins between human interferon gamma and human tumour necrosis factor alpha or beta have been constructed using site-directed mutagenesis. The genes were fused via a synthetic oligonucleotide linker coding for tetrapeptide Pro-Val-Gly-Pro. The fused genes were expressed in Escherichia coli under control of early promoters of bacteriophage T7. E. coli cells harbouring the plasmids with the hybrid genes gave rather high level of the fused proteins biosynthesis. The hybrid recombinant proteins proved to be unstable in E. coli cells.
authors.forename: V G
authors.initials: VG
authors.lastname: Korobko
authors.forename: I V
authors.initials: IV
authors.lastname: Davydov
authors.forename: L N
authors.initials: LN
authors.lastname: Shingarova
authors.forename: S A
authors.initials: SA
authors.lastname: Filippov
authors.forename: D S
authors.initials: DS
authors.lastname: Esipov
authors.forename: N P
authors.initials: NP
authors.lastname: Berkova
authors.forename: V N
authors.initials: VN
authors.lastname: Dobrynin
mesh_terms: D001483:Base Sequence; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011995:Recombination, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma
vernacular_title: Geny gibridnykh limfokinov cheloveka. I. Konstruirovanie rekombinantnykh plazmid, kodiruiushchikh gibridy immunnogo interferona i faktorov nekroza opukholeĭ cheloveka.