title: Immunocytochemical localization of neutrophil elastase in term placenta decidua and myometrium in pregnancy-induced hypertension.
abstract: OBJECTIVE
  The aim of the study was to determine whether there was evidence of elastase containing neutrophils at the materno-fetal interface in women with pregnancy-induced hypertension (PIH).
  
  
  METHODS
  An observational prospective study.
  
  
  METHODS
  The Simpson Memorial Maternal Pavilion Edinburgh.
  
  
  METHODS
  Placentas were obtained at vaginal or abdominal delivery from 51 consecutive women, 23 had normal pregnancies (13 caesarean sections) and 28 had PIH (18 caesarean sections). An immunocytochemical technique was used to localize elastase containing neutrophils in the placenta, decidua and myometrium.
  
  
  METHODS
  The numbers of positively stained cells, estimated subjectively as minimal, moderate or heavy, in subchorionic plate, perivillous fibrin and decidua.
  
  
  RESULTS
  In both normal and PIH pregnancies neutrophils were absent from the myometrium. However, elastase containing neutrophils were located in areas of fibrin in the subchorionic plate and around the villi although there was no significant difference between the normal and PIH group. Neutrophils were also located in the fibrin of the decidua and in this case the number was significantly greater in the PIH group than in the normal group and correlated with plasma urate.
  
  
  CONCLUSIONS
  The release of neutrophil elastase in the decidua could contribute to the vascular damage evident in PIH.
authors.affiliation: University Department of Obstetrics, Centre for Reproductive Biology, Edinburgh.
authors.forename: B H
authors.initials: BH
authors.lastname: Butterworth
authors.forename: I A
authors.initials: IA
authors.lastname: Greer
authors.forename: W A
authors.initials: WA
authors.lastname: Liston
authors.forename: N G
authors.initials: NG
authors.lastname: Haddad
authors.forename: T A
authors.initials: TA
authors.lastname: Johnston
mesh_terms: D000328:Adult; D003656:Decidua / Q000201:enzymology*; D005260:Female; D006801:Humans; D006973:Hypertension / Q000201:enzymology*; D007150:Immunohistochemistry; D009215:Myometrium / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000032:analysis*; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D011249:Pregnancy Complications, Cardiovascular / Q000201:enzymology*
chemical_list: D010196:Pancreatic Elastase