Protein analysis of Theileria sergenti/buffeli/orientalis piroplasms by two-dimensional polyacrylamide gel electrophoresis.
Proteins of the piroplasms of Theileria sergenti, T. buffeli and T. orientalis were analysed by two-dimensional polyacrylamide gel electrophoresis. Protein spot patterns of T. buffeli and T. orientalis were identical except for a few minor proteins, whereas spot patterns of two T. sergenti stocks were differentiated from those of T. buffeli and T. orientalis by a characteristic set of proteins including a major protein of molecular weight 33-34 kDa. This result indicates that Japanese T. sergenti can be phenotypically distinguishable from European and Australia Theileria species; T. orientalis and T. buffeli.
Sugimoto
C
C
National Institute of Animal Health, Ibaraki, Japan.
Kawazu
S
S
Kamio
T
T
Fujisaki
K
K
D000818:Animals; D016782:Apicomplexa / Q000032:analysis*; D001798:Blood Proteins / Q000032:analysis; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000737:chemistry; D007525:Isoelectric Focusing; D008565:Membrane Proteins / Q000032:analysis; D015800:Protozoan Proteins / Q000032:analysis*; D013801:Theileriasis / Q000469:parasitology
D001798:Blood Proteins; D008565:Membrane Proteins; D015800:Protozoan Proteins