Tyrosinase expression and melanogenesis in melanotic and amelanotic B16 mouse melanoma cells.
Tyrosinase activity, abundance, and mRNA transcription were examined in three sublines of the B16 mouse melanoma. Tyrosinase activity and melanin content were highest in the B16-F1 cells, slightly less in the B16-F10, and markedly lower in the B16-F10-DD cells. No differences in the level of tyrosinase mRNA or protein were found in the three different sublines. Thus, the differences in tyrosinase expression arise from the post-translational modification of the enzyme causing its activation or inhibition.
Burchill
S A
SA
Department of Dermatology, University of Newcastle upon Tyne, UK.
Bennett
D C
DC
Holmes
A
A
Thody
A J
AJ
D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division; D015870:Gene Expression; D008543:Melanins / Q000032:analysis / Q000378:metabolism*; D008546:Melanoma, Experimental / Q000737:chemistry / Q000201:enzymology / Q000473:pathology*; D051379:Mice; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000378:metabolism*; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000201:enzymology / Q000473:pathology
D008543:Melanins; D012333:RNA, Messenger; D014442:Monophenol Monooxygenase