[The Mopeia virus induces pH-dependent "fusion from within" in a BHK-21 cell culture].
The capacity of BHK-21 cell culture to produce Mopeia virus (Arenaviridae family) to form syncytium upon acidification of the culture medium to pH 5.5 and lower was demonstrated. The cell fusion requires their active virus production: a virus titre in the culture medium must be at least 10(5) PEU/ml. The inhibition of virus multiplication with ammonium chloride as well as treatment of the cells before the medium acidification with immune serum reduced syncytium formation markedly. No cell fusion was observed upon acidification of the medium immediately after virus adsorption to cells. Thus, the observed cell fusion under the influence of the virus is an "internal fusion" and confirms our previous data on the endocytosis mode of arenavirus penetration into the cell.
Glushakova
S E
SE
Vasiuchkova
A D
AD
Erokhina
I R
IR
Pyzhik
E V
EV
Kurash
T P
TP
D000327:Adsorption; D000535:Aluminum / Q000494:pharmacology; D000077410:Aluminum Chloride; D017607:Aluminum Compounds; D000818:Animals; D001116:Arenaviridae / Q000187:drug effects / Q000276:immunology / Q000472:pathogenicity*; D002459:Cell Fusion / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000382:microbiology; D002712:Chlorides / Q000494:pharmacology; D006224:Cricetinae; D015726:Giant Cells / Q000166:cytology / Q000187:drug effects / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera / Q000494:pharmacology; D014776:Virus Cultivation
D017607:Aluminum Compounds; D002712:Chlorides; D007106:Immune Sera; D000077410:Aluminum Chloride; D000535:Aluminum
Virus Mopeĭia indutsiruet pH-zavisimoe "sliianie iznutri" kul'tury kletok BHK-21.