Rapid enzymatic method for the measurement of mannitol in urine.
A previously described method for mannitol in urine has been modified and improved. End product inhibition by fructose in the mannitol dehydrogenase method for mannitol has been minimized; the assay is linear over a sample mannitol concentration range of 0-12 mmol/L; no significant interference from other sugars or sugar alcohols could be demonstrated. The method is precise (within-batch CV less than 1%), rapid and shows excellent recovery of mannitol in spiked samples. Comparison with gas liquid chromatography shows excellent correlation (r = 0.994) between the two methods.
Blood
J
J
Department of Chemical Pathology, Derbyshire Royal Infirmary, Derby, UK.
Ingle
A R
AR
Allison
N
N
Davies
G R
GR
Hill
P G
PG
D002849:Chromatography, Gas; D015510:Clinical Medicine / Q000379:methods*; D006593:Hexokinase / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008353:Mannitol / Q000652:urine*; D008354:Mannitol Dehydrogenases
D008353:Mannitol; D008354:Mannitol Dehydrogenases; D006593:Hexokinase