Inhibitory effects of coffee on the genotoxicity of carcinogens in mice.
The mouse bone marrow micronucleus test was carried out to evaluate the possible inhibitory effects of 3 doses (125, 250 and 500 mg/kg) of standard instant coffee on the in vivo genotoxicity of 7,12-dimethylbenz[a]anthracene (DMBA), benzo[a]pyrene (BP), aflatoxin B1 (AFB1) and urethane (UR). Coffee was orally administered twice, 2 and 20 h before the carcinogens were injected intraperitoneally. From the results obtained, it was evident that the administration of 250 and 500 mg coffee/kg body weight could significantly inhibit the in vivo genotoxicity of these carcinogens. A linear dose response was observed for the inhibitory effect of coffee. Furthermore, inhibition of genotoxicity by coffee was observed in bone marrow cells which were sampled at 6-h intervals (48, 54, 60, 66 and 72 h) from the time of peak induction of micronuclei by DMBA.
Abraham
S K
SK
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D016604:Aflatoxin B1; D000348:Aflatoxins / Q000633:toxicity; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D001853:Bone Marrow / Q000187:drug effects; D002273:Carcinogens / Q000633:toxicity*; D003069:Coffee; D003520:Cyclophosphamide / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects*; D014520:Urethane / Q000633:toxicity
D000348:Aflatoxins; D002273:Carcinogens; D003069:Coffee; D001564:Benzo(a)pyrene; D014520:Urethane; D015127:9,10-Dimethyl-1,2-benzanthracene; D003520:Cyclophosphamide; D016604:Aflatoxin B1