title: Activated neutrophils inhibit cerebrovascular endothelium-dependent relaxations in vitro.
abstract: The effect of formyl-Met-Leu-Phe- (fMLP-) activated feline neutrophil granulocytes on endothelium-dependent and independent relaxations was studied in the middle cerebral artery of the cat in vitro. Endothelium-dependent relaxations caused by acetylcholine and ATP were markedly inhibited after 30 minutes of incubation of the vessels with neutrophils (5000 cells/microliter) in the presence of 5 microM fMLP, followed by a replacement of the bath solution in order to remove the neutrophils from the medium. Direct vasorelaxations in response to the nitric oxide donor compound SIN-1, however, remained unchanged. Both neutrophils and fMLP caused transient contractions during the incubation period. The present study provides direct evidence for the ability of activated neutrophils to cause an inhibition of vascular endothelium-dependent responses in vitro.
authors.affiliation: Department of Neurology, University of Pennsylvania, Philadelphia 19104-6063.
authors.forename: C
authors.initials: C
authors.lastname: Csaki
authors.forename: C
authors.initials: C
authors.lastname: Szabo
authors.forename: Z
authors.initials: Z
authors.lastname: Benyo
authors.forename: M
authors.initials: M
authors.lastname: Reivich
authors.forename: A G
authors.initials: AG
authors.lastname: Kovach
mesh_terms: D000818:Animals; D002415:Cats; D002536:Cerebral Arteries / Q000502:physiology; D004730:Endothelium, Vascular / Q000502:physiology*; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000187:drug effects; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000502:physiology*; D009569:Nitric Oxide / Q000502:physiology; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology*; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D014665:Vasodilator Agents; D009569:Nitric Oxide; D009240:N-Formylmethionine Leucyl-Phenylalanine