Immunological recognition of larval Taenia crassiceps glycolipids by sera from parasite-infected mice.
The isolation and purification of a neutral glycolipid fraction from Taenia crassiceps metacestodes (KBS strain), harvested from both male and female NMRI mice at 70-80 days following intraperitoneal infection, revealed 24 thin-layer chromatography-designated glycolipid bands. The glycolipids were defined as ceramide mono- (n = 3), di- (n = 3), tri- (n = 4), tetra- (n = 5), and greater than tetrasaccharides (n = 9) according to their running properties as defined by thin-layer chromatography against standards of known structure. The defined glycolipids were tested for immunoreactivity with sera from noninfected and T. crassiceps-infected NMRI mice (intraperitoneal injection or implantation of 15 larvae/animal) using the enzyme-linked immunosorbent assay (ELISA) until day 33 p.i. (IgM and IgG reaction) and high-performance thin-layer chromatography (HPTLC) combined with immunostaining (IgG reaction) until day 7 p.i. ELISA-determined IgM and IgG titres were significantly elevated from day 5 p.i. Immunostaining revealed early reactivity for certain ceramide tetra- and greater than tetrasaccharides (n = 6) on day 3 p.i. From day 5 p.i. onwards, nearly all glycolipids, including ceramide mono- and disaccharides, were recognized by the sera from metacestode-challenged mice. On day 7 p.i., a total of 22 bands were serologically active; of these, a considerable number (n = 10) showed increased staining intensity. Remarkably, in many cases (10 of 20), 3 glycolipids (tetra- and greater than tetrasaccharides) were weakly recognized by mouse sera taken before infection.
Kunz
J
J
Fachbereich Biologie, Philipps-Universität Marburg, Federal Republic of Germany.
Baumeister
S
S
Dennis
R D
RD
Küytz
B
B
Wiegandt
H
H
Geyer
E
E
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006017:Glycolipids / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D008297:Male; D051379:Mice; D013621:Taenia / Q000276:immunology*; D013622:Taeniasis / Q000276:immunology*
D000909:Antibodies, Helminth; D006017:Glycolipids; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M