title: Purification, crystallization and preliminary crystallographic analysis of porcine aldose reductase.
abstract: Large crystals of porcine aldose reductase have been grown from polyethylene glycol solutions. The crystals are triclinic, space-group P1, with a = 81.3 A, b = 85.9 A, c = 56.6 A, alpha = 102.3 degrees, beta = 103.3 degrees and gamma = 79.0 degrees. The crystals grow within ten days to dimensions of 0.6 mm x 0.4 mm x 0.2 mm and diffract to at least 2.5 A. There are four molecules in the unit cell related by a set of three mutually perpendicular non-crystallographic 2-fold axes.
authors.affiliation: University of Alabama, Center for Macromolecular Crystallography, Birmingham 35294.
authors.forename: O
authors.initials: O
authors.lastname: el-Kabbani
authors.forename: S V
authors.initials: SV
authors.lastname: Narayana
authors.forename: Y S
authors.initials: YS
authors.lastname: Babu
authors.forename: K M
authors.initials: KM
authors.lastname: Moore
authors.forename: T G
authors.initials: TG
authors.lastname: Flynn
authors.forename: J M
authors.initials: JM
authors.lastname: Petrash
authors.forename: E M
authors.initials: EM
authors.lastname: Westbrook
authors.forename: L J
authors.initials: LJ
authors.lastname: DeLucas
authors.forename: C E
authors.initials: CE
authors.lastname: Bugg
mesh_terms: D000449:Aldehyde Reductase / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D011487:Protein Conformation; D013552:Swine; D014961:X-Ray Diffraction
chemical_list: D000449:Aldehyde Reductase