[Use of DNA amplification by PCR in the study of the hypervariable region (VNTR) in a forensic medicine setting. Experience with 2 systems: Apo B and YNZ 22].
The PCR method has been applied to amplify two Variable-number-Tandem-Repeat (VNTR) sequences. The high polymorphism of these VNTR systems can be usefully applied in medical legal fields such as paternity testing and individual identification. The VNTR systems utilized were: ApoB and YNZ 22. The study was conducted on a three-generation family of thirteen members, whose relationship was previously established using conventional blood systems. The results confirm the Mendelian inheritance of the alleles found and the suitability of the PCR method for forensic purposes.
Giorgetti
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Istituto di Medicina Legale, l'Università di Ancona.
Tagliabracci
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A
Cingolani
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Ferrara
S D
SD
D000483:Alleles; D001055:Apolipoproteins B / Q000235:genetics*; D001483:Base Sequence; D004276:DNA, Satellite / Q000032:analysis; D005069:Evaluation Studies as Topic; D005554:Forensic Medicine / Q000379:methods*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010334:Paternity; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
D001055:Apolipoproteins B; D004276:DNA, Satellite; D009838:Oligodeoxyribonucleotides
La utilizzazione dell' amplificazione del DNA mediante PCR nello studio di regioni ipervariabili VNTR in ambito medico-forense. Esperienza con due sistemi: ApoB e YNZ 22.