Recognition of HLA-B27 by mouse cytotoxic T-cell clones: a transgenic mouse model.
As a basis for the characterization of mouse T cells involved in the recognition of xenogeneic HLA molecules, a panel of HLA-B27-reactive cytotoxic T-cell clones was generated upon stimulation by cells from HLA-B27-transgenic mice. The HLA-B27-induced T-cell response was found to comprise two categories of clones: some recognizing HLA-B27 independent of H-2 molecules expressed by the target cells (unrestricted clones), others recognizing HLA-B27 in an H-2-restricted manner. The unrestricted clones exhibited diverse specificities, as judged from their various cross-reactivities with other xenogeneic (HLA) or allogeneic (H-2) molecules. In addition, although most of the unrestricted clones were able to react with both mouse and human HLA-B27-transgenic mice. The HLA-B27-induced T-cell which reacted only with HLA-B27-positive mouse, and not human cells. These findings illustrate that both H-2-restricted and unrestricted T cells with diverse specificities contribute to HLA-B27-xenorecognition.
Reboul
M
M
Mouse Immunogenetics, U93 INSERM, Saint-Louis Hospital, Paris, France.
Frangoulis
B
B
Rocca
A
A
Degos
L
L
Pla
M
M
D000818:Animals; D002999:Clone Cells; D006183:H-2 Antigens / Q000276:immunology; D015796:HLA-B27 Antigen / Q000235:genetics / Q000276:immunology*; D006801:Humans; D051379:Mice; D008809:Mice, Inbred C3H; D008822:Mice, Transgenic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D001613:beta 2-Microglobulin / Q000276:immunology
D006183:H-2 Antigens; D015796:HLA-B27 Antigen; D001613:beta 2-Microglobulin