In vivo and in vitro effect of Cantastim, an immunomodulatory agent extracted from a highly pathogenic Pseudomonas aeruginosa strain.
Cantastim is a biological product consisting of distinct molecules of phospholipids obtained from a peculiar Pseudomonas aeruginosa strain. It enhanced the IgM and especially the IgG responses of mice pretreated with Cantastim and then intraperitoneally inoculated with T-dependent antigens such as sheep erythrocytes; restored cellular immunocompetence as evidenced by an increase in the in vitro proliferative responses to T-cell mitogens and allogeneic stimuli of murine splenocytes from in vivo pretreated mice activated NK cytotoxicity, and inhibited in vivo growth of the Ehrlich ascites tumor. In vitro it stimulated the mitogenesis of mouse lymphocytes but did not exert such stimulatory effect on guinea pig or human lymphocytes. All these findings allow to define Cantastim as an immunomodulating agent influencing mainly the cell-mediated immune response.
Olinescu
A
A
Department of Cellular Immunology, Cantacuzino Institute, Bucharest, Romania.
Hristescu
S
S
Sålågeanu
A
A
Manda
G
G
Neagu
M
M
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation; D000970:Antineoplastic Agents; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D010743:Phospholipids; D010835:Phytohemagglutinins / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000032:analysis*
D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010743:Phospholipids; D010835:Phytohemagglutinins; C049675:cantastim