An Anaplasma centrale DNA probe that differentiates between Anaplasma ovis and Anaplasma marginale DNA.
An Anaplasma centrale genomic library was constructed in pUC13. Two clones pAC5 and pAC137 hybridising to A. centrale and A. marginale DNA were isolated from this library. One of these, pAC5, also hybridised to DNA from A. ovis. The total insert of pAC5 was subcloned into pBR322. This subclone, pAC5-12, could detect 1 ng A. centrale, 0.5 ng A. marginale and 3.9 ng A. ovis DNA. The hybridisation pattern obtained with pAC5-12 on digests of A. centrale, A. marginale and A. ovis DNA suggests that this probe detects EcoR1 and Hind111-polymorphisms. Probe pAC5-12 could detect A. ovis DNA in 36% of blood samples tested compared to the 33% detectability obtained with microscopy.
Visser
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Molecular Biology Section, Veterinary Research Institute, Onderstepoort, South Africa.
Ambrosio
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de Waal
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D000709:Anaplasma / Q000235:genetics* / Q000302:isolation & purification; D000712:Anaplasmosis / Q000175:diagnosis*; D000818:Animals; D015139:Blotting, Southern; D002417:Cattle; D015342:DNA Probes; D004269:DNA, Bacterial / Q000097:blood*; D015723:Gene Library; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping
D015342:DNA Probes; D004269:DNA, Bacterial