NADH:ubiquinone oxidoreductase from bovine heart mitochondria. Complementary DNA sequence of the import precursor of the 10 kDa subunit of the flavoprotein fragment.
The amino acid sequence of the 10 kDa subunit of the flavoprotein (FP) fragment of complex I from bovine heart mitochondria has been determined by protein sequence analysis, thereby completing the sequence of the FP fragment. The calculated molecular weight of the 10 kDa subunit agrees exactly with the value of 8438 determined by electrospray mass spectrometry, and further confirmation of the sequence has been obtained by sequencing cDNAs amplified from total bovine heart cDNA by the polymerase chain reaction, using mixed oligonucleotides based upon the protein sequence as primers and hybridization probes. The sequence of the 10 kDa subunit is not related to that of any known protein. Being devoid of cysteine residues, it has none of the characteristic features of known iron-sulfur proteins and it is improbable that it is involved in liganding Fe-S centers in the FP fragment.
Skehel
J M
JM
Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
Pilkington
S J
SJ
Runswick
M J
MJ
Fearnley
I M
IM
Walker
J E
JE
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003545:Cysteine / Q000737:chemistry; D004247:DNA; D005420:Flavoproteins / Q000737:chemistry / Q000235:genetics*; D007506:Iron-Sulfur Proteins / Q000737:chemistry; D013058:Mass Spectrometry; D008929:Mitochondria, Heart / Q000201:enzymology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016660:NAD(P)H Dehydrogenase (Quinone); D010446:Peptide Fragments / Q000235:genetics; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D011808:Quinone Reductases / Q000235:genetics* / Q000378:metabolism
D000596:Amino Acids; D005420:Flavoproteins; D007506:Iron-Sulfur Proteins; D010446:Peptide Fragments; D011498:Protein Precursors; D004247:DNA; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases; D003545:Cysteine