High-performance capillary electrophoresis of gangliosides.
Gangliosides are sialic acid-containing glycosphingolipids. In aqueous media, these glycolipids have been shown to exist as stable micelles. Ganglioside micelles could be analyzed by high-performance zonal capillary electrophoresis in uncoated fused-silica capillaries within 10 min. The mass sensitivity determined by monitoring the absorption of ultraviolet light at 195 nm was in the order of 10(-11) mol. Increasing the pH of the running buffer from 3.0 to 7.4 or the voltage from 10 to 30 kV increased the relative mobilities of gangliosides. By contrast, increasing the ionic strength of the buffer decreased the migration and broadened the elution peak widths of gangliosides. Ganglioside* micelles including GM1, GD1b, and GT1b were resolved into separate peaks by capillary electrophoresis at physiological pH shortly after mixing. Upon prolonged incubation, the ganglioside peaks merged to form a single species. The fusion process was temperature-dependent. At 50 degrees C, formation of mixed micelles between polysialogangliosides GD1b and GT1b was complete within 30 min. In contrast, no fusion of the ganglioside peaks was observed at 0 degrees C even after 75 h. Formation of mixed micelles between GD1b and other polysialogangliosides including GD1a, GT1b, and GQ1b at 37 degrees C required 1.5, 3.0, and 2.0 h, respectively. Formation of mixed micelles between monosialoganglioside GM1 and polysialogangliosides were 6- to 36-fold slower. No fusion was observed between monosialogangliosides GM1 and GM2 after 2 days of incubation. These findings indicate that polysialogangliosides may have higher propensities than monosialoganglioside to form mixed micelles.
Liu
Y B
YB
Laboratory of Experimental Neuropathology, National Institute of Neurological Disorders and Stroke, Bethesda, MD.
Chan
K F
KF
D004586:Electrophoresis / Q000379:methods; D005677:G(M1) Ganglioside / Q000737:chemistry; D005678:G(M2) Ganglioside / Q000737:chemistry; D005679:G(M3) Ganglioside / Q000737:chemistry; D005732:Gangliosides / Q000737:chemistry*; D007700:Kinetics; D008823:Micelles
D005679:G(M3) Ganglioside; D005732:Gangliosides; D008823:Micelles; D005678:G(M2) Ganglioside; D005677:G(M1) Ganglioside