Protein kinase C activity and intracellular distribution in surgically excised human epileptic neocortex.
Protein kinase C (PKC) activity assayed by phosphorylation of exogenous histone, was measured in neocortex obtained from 32 patients following surgery for focal epilepsy and from 6 non-epileptic patients. PKC activity was not significantly different in either the particulate or cytosolic fraction from epileptic foci (n = 17) versus samples from non-spiking regions (n = 22) or neocortex from non-epileptic patients (n = 6). From 67% to 70% of total PKC activity was present in the cytosolic fraction. Phosphorylation of endogenous cytosolic substrate proteins was also not significantly different in epileptic foci.
Vernet
O
O
Montreal Neurological Institute, McGill University, Qué, Canada.
Yong
V W
VW
Rostworowski
K
K
Fadich
M
M
Olivier
A
A
Sherwin
A L
AL
D001706:Biopsy; D002540:Cerebral Cortex / Q000201:enzymology* / Q000473:pathology; D004827:Epilepsy / Q000201:enzymology*; D006801:Humans; D011493:Protein Kinase C / Q000378:metabolism*
D011493:Protein Kinase C