Analysis of Bordetella pertussis cya operon regulation by use of cya-lac fusions.
In Bordetella pertussis virulence-associated genes, including adenylate cyclase toxin (Cya), are coordinately regulated in response to environmental signals by proteins coded by the bvg-locus. We have constructed cya-lac fusions in Escherichia coli and have shown that the cya operon is not expressed in E. coli, neither is it activated by bvg, when introduced in trans. The cya-lac fusion is fully active when returned to B. pertussis by homologous recombination and responds to bvg-dependent activation and environmental regulation. These results indicate that in B. pertussis the activation of the cya operon by bvg is indirect.
Goyard
S
S
Département de Biochimie et Génétique Moléculaire, Institut Pasteur, Paris, France.
Ullmann
A
A
D037361:Adenylate Cyclase Toxin; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001886:Bordetella pertussis / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D009876:Operon; D010957:Plasmids; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014774:Virulence / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism
D037361:Adenylate Cyclase Toxin; D001426:Bacterial Proteins; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase; D001618:beta-Lactamases