Application of FRIT fast atom bombardment liquid chromatography/mass spectrometry for the determination of acetylcholine levels in rat brain regions.
This report describes the use of FRIT fast atom bombardment (FAB) liquid chromatography/mass spectrometry for the analysis of acetylcholine in rat brain regions. Direct assessment of acetylcholine levels is possible without the need for either derivatization or extensive sample preparation. Quantification is accomplished by monitoring intact molecular cations of acetylcholine and a deuterated internal standard. The results are compared with those obtained by conventional pyrolysis gas chromatography/mass spectrometry and by liquid chromatography with electrochemical detection.
Ikarashi
Y
Y
Department of Neuropsychopharmacology (Tsumura), Gunma University, School of Medicine, Japan.
Itoh
K
K
Maruyama
Y
Y
D000109:Acetylcholine / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D016339:Spectrometry, Mass, Fast Atom Bombardment
D000109:Acetylcholine