[Effect of the artificial electron acceptor reumycin on the nature of an intermediate accumulated by Pseudomonas aeruginosa bacteria].
We investigated the effect of the autooxidizable electron acceptor reumicin on the oversynthesis of intermediates by the bacterium Pseudomonas aeruginosa during its growth limitation by a nitrogen source. Addition of reumicin which accepted the reducing equivalents from NADH(+)-dehydrogenase of the respiratory chain was shown to reduce the rate of alpha-ketoglutarate formation and yield, and to induce the synthesis and excretion of pyruvate into the cultivation medium. The maximal accumulation of pyruvate was observed when reumicin was added at a concentration of 0.4-0.5 mM. It is concluded that the nature of the overproduced intermediate, the rate of its biosynthesis and yield depend on the extent of reducibility of the pyridine nucleotide pool. The oversynthesis of alpha-ketoglutarate is suppressed by chloramphenicol, a protein synthesis inhibitor, which points to a possible involvement of inducible systems including the carriers of the acids via the cytoplasmic membrane.
Trutko
S M
SM
Akimenko
V K
VK
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004579:Electron Transport / Q000502:physiology; D007656:Ketoglutaric Acids / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000378:metabolism; D010084:Oxidation-Reduction; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D014227:Triazines / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D007656:Ketoglutaric Acids; D011773:Pyruvates; D014227:Triazines; C009169:reumycin; D019289:Pyruvic Acid; D009245:NADH Dehydrogenase
Vliianie reumitsina--iskusstvennogo aktseptora élektronov na prirodu intermediata, nakaplivaemogo bakteriiami Pseudomonas aeruginosa.