Further characterization of the 5'-flanking DNA of the gene encoding human plasminogen activator inhibitor-1.
Previous nucleotide (nt) sequence analysis of the 5'-flanking DNA of the gene (PAI-1) encoding plasminogen activator inhibitor-1 revealed an extensive region of shared nt sequence identity with the 5'-flanking region of the gene (t-PA) encoding tissue-type plasminogen activator [Bosma et al., J. Biol. Chem. 263 (1988) 9129-9141]. Additional sequence (1642 bp) from the PAI-1 gene 5'-flanking region reveals that these 'PAI-1/t-PA' sequence elements share an alignment that contains a total of 575 positions. This additional PAI-1 5'-flanking sequence also contains two Alu elements that form inverted repeats. Southern-blot analysis using the PAI-1/t-PA element as a probe indicates that this element is repeated in the human genome. which supports the classification of this element as a medium reiteration frequency sequence [Jurka, Nucleic Acids Res. 18 (1990) 137-141].
Bosma
P J
PJ
Gaubius Institute TNO, Leiden The Netherlands.
Kooistra
T
T
Siemieniak
D R
DR
Slightom
J L
JL
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics*; D016208:Databases, Factual; D005796:Genes; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D015849:Plasminogen Inactivators; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D010959:Tissue Plasminogen Activator / Q000235:genetics
D015849:Plasminogen Inactivators; D004247:DNA; D010959:Tissue Plasminogen Activator