Apparent role of adenosine diphosphoribosyl transferase in the development of Mytilus edulis and the inhibition of differentiation by ligands of the enzyme protein.
The poly(ADP-ribose) polymerase or transferase (ADPRT) activity of developing embryos of Mytilus edulis increases with the progression of larval growth. ADPRT protein was partially purified from 2-hr-old embryos and identified by gel electrophoresis and immunotransblot, demonstrating cross-reactivity with anti-ADPRT IgG produced against the calf thymus enzyme. Two inhibitors of ADPRT, benzamide, competing with NAD at the nicotinamide binding site, and 6-amino-1,2-benzopyrone, which competes with DNA at the DNA binding site(s), both selectively arrest differentiation at the prodissoconch stage. The DNA site-oriented inhibitor, 6-amino-1,2-benzopyrone, has a much larger differentiation arresting effect than benzamide. The arrest of differentiation by 6-amino-1,2-benzopyrone is reversible. A probable ecotoxicity of ADPRT ligands on mussel differentiation is proposed.
Bauer
P I
PI
Laboratory for Environmental Toxicology and Chemistry, Romberg Tiburon Center, San Francisco State University, Tiburon, California 94920.
Kline
K
K
Kun
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E
D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D001667:Binding, Competitive; D049872:Bivalvia / Q000196:embryology*; D002454:Cell Differentiation / Q000187:drug effects; D003374:Coumarins / Q000494:pharmacology*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D008297:Male; D010063:Ovum / Q000378:metabolism; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000502:physiology*; D013094:Spermatozoa / Q000378:metabolism
D001549:Benzamides; D003374:Coumarins; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C037689:benzamide; D011065:Poly(ADP-ribose) Polymerases; C067496:6-amino-1,2-benzopyrone