[Measuring of intracellular Ca2+ concentration in macrophages. Effects of platelet activation factor].
In experiments on mice resident and stimulated thioglycolate macrophages the changes in cytoplasmic Ca2+ concentration Ca2+ have been studied by the use of the fluorescent probe fura-2. PAF acether (10(-7) M) raised Ca2+ by 300-400 nM within 1 min only in the stimulated macrophages. In the resident cells this increase was much less. In the presence of 2mM EGTA, PAF raised Ca2+ to a lesser extent. This suggests that PAF causes influx of exogenous Ca2+ through the receptor-mediated channels as well as releasing Ca2+ from intracellular stores.
Pinelis
V G
VG
Zagulova
D V
DV
Galkin
A A
AA
Baskakov
M B
MB
Markov
Kh M
KhM
D000818:Animals; D002118:Calcium / Q000032:analysis*; D003593:Cytoplasm / Q000737:chemistry; D005456:Fluorescent Dyes; D008264:Macrophages / Q000737:chemistry*; D051379:Mice; D010972:Platelet Activating Factor / Q000494:pharmacology*
D005456:Fluorescent Dyes; D010972:Platelet Activating Factor; D002118:Calcium
Izmerenie vnutrikletochnoĭ kontsentratsii Ca2+ v makrofagakh, vliianie faktora aktivatsii trombotsitov.