Effect of subchronic aflatoxin exposure on growth and progression of Ehrlich's ascites tumor in mice.
The present investigation was undertaken to assess whether aflatoxin B1 (AFB1) has any modulatory effect on the growth and progression of Ehrlich's ascites tumor (EAT) in mice or not. Male Swiss albino mice were treated with 0, 70, 350 and 700 micrograms AFB1/kg body weight in 0.2 ml corn oil on alternate days, orally, for two weeks. Treated animals were challenged with 1 x 10(6) cells of Ehrlich's ascites tumor. Animals were monitored for the appearance of palpable tumor, body weight gain as a measure of tumor burden, mortality profile and tumor cell population. Some parameters of cell mediated immunity (CMI), humoral immunity and non-specific immunity were also studied in aflatoxin treated animals in order to find out the mechanism of action of AFB1 on host immunity. It was observed that AFB1 treatment resulted in an early appearance of tumor, enhanced mortality, appreciable increase in body weight gain and EAT cell population following tumor challenge, in comparison with the control animals. Aflatoxin treatment caused suppression of CMI including an impairment of macrophage function, while humoral immunity was not much affected. It may be concluded that impaired CMI and macrophage function might be contributing to the increased growth of EAT in the AFB1 treated animals. Our findings may be relevant in that when a host is exposed to aflatoxins concomitantly with other carcinogens and cancer causing situations.
Raisuddin
S
S
Preventive Toxicology Division, Industrial Toxicology Research Centre, Lucknow, India.
Zaidi
S I
SI
Singh
K P
KP
Ray
P K
PK
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology / Q000401:mortality / Q000473:pathology*; D007109:Immunity / Q000187:drug effects; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced; D008221:Lymphoid Tissue / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000502:physiology; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D015430:Weight Gain / Q000187:drug effects
D000348:Aflatoxins; D002273:Carcinogens; D016604:Aflatoxin B1