Improved neutrophil separation from anti-D-treated Rh(D)-positive whole blood by a discontinuous gradient method.
Neutrophil separation in Rh(D)-positive whole blood with a discontinuous gradient method (Ficoll 1077-1119 and sodium diatrizoate) was improved by adding anti-D reagent to ethylene diaminetetraacetic acid anticoagulated blood (1 drop/mL). The mean yield was raised more than two times (x2.6) without red blood cell contamination. Anti-D reagent did not impair neutrophil viability, purity, nitroblue tetrazolium-formazan production, or shape change response to N-formyl-methionyl-leucinyl-phenyl-alanine.
Schinella
M
M
Laborator o di Chimica Clinica ed Ematologia dell'Ospedale Civile Maggiore, Verona, Italy.
D000328:Adult; D001773:Blood Cells / Q000166:cytology; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D009504:Neutrophils / Q000166:cytology*; D012016:Reference Values; D012204:Rh-Hr Blood-Group System
D012204:Rh-Hr Blood-Group System