Molecular analysis of MHC-class-I alterations in human tumor cell lines.
Molecular characterization of HLA-class-I expression was investigated in human tumor cell lines at the protein and mRNA levels using locus-specific monoclonal antibodies (MAbs) and probes. Some cell lines exhibited a differential expression of HLA-A and HLA-B products and also showed differences in the inducibility of HLA-class-I genes by gamma-IFN. Thus, gamma-IFN stimulation induced predominantly HLA-B mRNA in the HeP-2 cell line, which showed imbalances in basal levels of HLA-A and HLA-B expression. This unequal inducibility of HLA genes may imply that locus-specific regulatory mechanisms are involved in the expression of individual HLA products. The specific mechanism controlling the differential expression of HLA subsets appears to be independent of c-myc activity. Northern blot analysis found no relationship between c-myc mRNA levels and specific mRNA for HLA-A and HLA-B antigens.
Ruiz-Cabello
F
F
Servicio de Análisis Clínicos e Inmunología, Hospital Virgen de las Nieves, Universidad de Granada, Spain.
Perez-Ayala
M
M
Gomez
O
O
Redondo
M
M
Concha
A
A
Cabrera
T
T
Garrido
F
F
D000911:Antibodies, Monoclonal; D015152:Blotting, Northern; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005805:Genes, MHC Class I; D015234:HLA-A Antigens / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000235:genetics*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D009369:Neoplasms / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects
D000911:Antibodies, Monoclonal; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015395:Histocompatibility Antigens Class I; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate