title: Diastereoselective hydrolysis of peptide esters by alkaline protease. Preparation of racemization-free peptides.
abstract: A new and practical enzymatic procedure for preparative diastereoselective hydrolysis of peptide esters using the alkaline protease alcalase as a catalyst was developed. This procedure has been successfully applied to the resolution of peptide diastereomers and synthesis of racemization free peptides.
authors.affiliation: Graduate Institute of Biochemical Sciences, National Taiwan University.
authors.forename: S T
authors.initials: ST
authors.lastname: Chen
authors.forename: S H
authors.initials: SH
authors.lastname: Wu
authors.forename: K T
authors.initials: KT
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D002384:Catalysis; D004952:Esters / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity; D013381:Subtilisins / Q000378:metabolism*
chemical_list: D004952:Esters; D010455:Peptides; D013381:Subtilisins