title: In vivo response of secretory component in the rat uterus to antigen, IFN-gamma, and estradiol.
abstract: Intrauterine immunization of ovariectomized rats with SRBC is known to elicit pronounced IgA and IgG antibody responses in uterine secretions of immunized uteri. To determine whether secretory component (SC), the receptor for transporting polymeric IgA from tissues to mucosal surfaces, was also influenced by Ag, ovariectomized rats were immunized and boosted by placing SRBC into the lumena of individual uterine horns. In response to Ag, the levels of polymeric IgA, as well as free SC and SC bound to polymeric IgA, increased in uterine secretions. When ovariectomized animals were treated with estradiol, a fivefold increase in SC levels was observed in the immunized horns, indicating that a hormone response is superimposed on the Ag-induced stimulation of uterine SC. To determine whether IFN-gamma influences the presence of SC in uterine secretions, IFN-gamma was placed in the uterine lumena of ovariectomized nonimmunized rats. When uterine secretions were analyzed, significantly higher levels of SC were found in IFN-gamma-exposed uteri than were present in saline treated control animals. In contrast, intrauterine instillation of IFN-gamma had no effect on the levels of IgA in uterine secretions. This response was specific for IFN-gamma in that IFN-alpha/beta had no effect on uterine SC or IgA levels. These results indicate that intrauterine instillation of Ag, in addition to evoking pronounced antibody responses, stimulates the production of SC, which may be responsible for the transport of polymeric IgA from tissue to uterine secretions. Furthermore, they indicate that IFN-gamma placed in the uterine lumen stimulates SC production and suggest that the uterine SC response to Ag may be mediated by the action of IFN-gamma on uterine epithelial cells.
authors.affiliation: Department of Physiology, Dartmouth Medical School, Hanover, NH 03756.
authors.forename: C R
authors.initials: CR
authors.lastname: Wira
authors.forename: J E
authors.initials: JE
authors.lastname: Bodwell
authors.forename: R H
authors.initials: RH
authors.lastname: Prabhala
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D002417:Cattle; D004912:Erythrocytes / Q000276:immunology; D004958:Estradiol / Q000502:physiology*; D005260:Female; D007070:Immunoglobulin A / Q000032:analysis; D007371:Interferon-gamma / Q000502:physiology*; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D012635:Secretory Component / Q000096:biosynthesis*; D012756:Sheep; D014599:Uterus / Q000276:immunology* / Q000378:metabolism
chemical_list: D000941:Antigens; D007070:Immunoglobulin A; D012635:Secretory Component; D004958:Estradiol; D007371:Interferon-gamma