Low copy number vectors for expression of fused genes to beta-galactosidase in Escherichia coli.
A series of six expression vectors, pXM184Lac.A, B, C, pXM184Z.A, B, C, based on the low copy plasmid pACYC184 that allow for expression of proteins fused to beta-galactosidase in Escherichia coli is described. A level of 50,000 units of beta-galactosidase is routinely observed and is easily identifiable on protein gels. This paper also reports the tight regulation of expression of the Trc promoter in these vectors using the LacIq repressor.
Bingham
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AH
Division of Biologics, PHLS Centre for Applied Microbiology and Research, Salisbury, Wiltshire, U.K.
D000595:Amino Acid Sequence; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D016153:Genes, Suppressor; D005822:Genetic Vectors; D007700:Kinetics; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase