Immobilisation of beta-D-galactosidase from Escherichia coli on cellulose beads and its use for the synthesis of disaccharide derivatives.
beta-D-Galactosidase, isolated from cloned E. coli, was immobilised on cellulose beads via oxidation with sodium periodate, activation by cyanuric chloride, or diazotisation. beta-D-Galactosidase immobilised via azo bonds showed the highest relative activity and thermostability, and was used for synthesis of disaccharide methyl glycosides.
Kéry
V
V
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Kucár
S
S
Matulová
M
M
Haplová
J
J
D002482:Cellulose; D004187:Disaccharides / Q000096:biosynthesis*; D004355:Drug Stability; D004800:Enzymes, Immobilized; D004926:Escherichia coli / Q000201:enzymology; D006358:Hot Temperature; D001616:beta-Galactosidase / Q000302:isolation & purification / Q000378:metabolism*
D004187:Disaccharides; D004800:Enzymes, Immobilized; D002482:Cellulose; D001616:beta-Galactosidase