Differences in the effects of piperine and piperonyl butoxide on hepatic drug-metabolizing enzyme system in rats.
An i.p. administration of rats with piperine (100 mg/kg) and piperonyl butoxide (400 mg/kg) produced a significant decrease in hepatic cytochrome P-450, and activities of benzphetamine N-demethylase, aminopyrine N-demethylase and aniline hydroxylase 1 hr after the treatment. Twenty-four hr later, these parameters along with cytochrome b5 and NADPH-cytochrome c reductase remained depressed only in piperine-treated rats. In contrast, piperonyl butoxide caused a significant induction of these parameters with the exception of cytochrome b5 and aminopyrine N-demethylase, which were up by 36 and 33% over their respective controls but not significantly. These results point up that effect of piperine on hepatic mixed-function oxidases is monophasic while that of piperonyl butoxide is biphasic.
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Toxicology Laboratory School of Veterinary Medicine, Tuskegee University, Alabama 36088.
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D000470:Alkaloids; D000818:Animals; D052117:Benzodioxoles; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D006899:Mixed Function Oxygenases / Q000032:analysis*; D010880:Piperidines / Q000494:pharmacology*; D010882:Piperonyl Butoxide / Q000494:pharmacology*; D053284:Polyunsaturated Alkamides; D051381:Rats; D011919:Rats, Inbred Strains
D000470:Alkaloids; D052117:Benzodioxoles; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D010882:Piperonyl Butoxide; C008922:piperine