Expression of epidermal growth factor mRNA in the developing mouse mandibular process.
Reverse transcription and cDNA amplification (polymerase chain reaction) of total RNA preparations were used to characterize the expression of EGF mRNA in the mandibular arch of day 9-17 mouse embryos. EGF mRNA was present in mandibles at day 9 and 10 but not at days 11-17. Separate RNA preparations from epithelium and mesenchyme at day 10 revealed EGF mRNA in both components.
Kronmiller
J E
JE
Department of Biostructure and Function, School of Dental Medicine, University of Connecticut Health Center, Farmington 06032.
Upholt
W B
WB
Kollar
E J
EJ
D000818:Animals; D004270:DNA, Circular; D004815:Epidermal Growth Factor / Q000235:genetics*; D004848:Epithelium / Q000737:chemistry / Q000196:embryology; D005784:Gene Amplification; D015870:Gene Expression; D005865:Gestational Age; D008334:Mandible / Q000737:chemistry / Q000196:embryology*; D008648:Mesoderm / Q000737:chemistry; D051379:Mice; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D014158:Transcription, Genetic
D004270:DNA, Circular; D012333:RNA, Messenger; D004815:Epidermal Growth Factor