Hydrolysis of carbaryl by a Pseudomonas sp. and construction of a microbial consortium that completely metabolizes carbaryl.
Two Pseudomonas spp. (isolates 50552 and 50581) isolated from soil degraded 1-naphthol and carbaryl, an N-methylcarbamate pesticide, respectively. They utilized these compounds as a sole source of carbon. 1-Naphthol was completely metabolized to CO2 by the isolate 50552, while the carbaryl was first hydrolyzed to 1-naphthol and then converted into a brown-colored compound by the isolate 50581. The colored metabolite was not degraded, but 1-naphthol produced by the isolate 50581 during the exponential phase of growth was metabolized by the isolate 50552. The two isolates were used to construct a bacterial consortium which completely catabolized carbaryl to CO2. No metabolite was detected in the cell cultures of the consortium. The isolate 50581 harbored a 50-kb plasmid pCD1, while no plasmid was detected in the isolate 50552. The isolated bacteria individually or as a consortium may be used for detoxification of certain industrial and agricultural wastes.
Chapalamadugu
S
S
Department of Biological Sciences, Oakland University, Rochester, Michigan 48309-4401.
Chaudhry
G R
GR
D001673:Biodegradation, Environmental; D012721:Carbaryl / Q000378:metabolism*; D006868:Hydrolysis; D009284:Naphthols / Q000378:metabolism; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D012988:Soil Microbiology
D009284:Naphthols; C029350:1-naphthol; D012721:Carbaryl