Molecular size and symmetry of Pseudomonas aeruginosa catabolic ornithine carbamoyltransferase. An X-ray crystallography analysis.
The catabolic ornithine carbamoyltransferase (EC 2.1.3.3) from Pseudomonas aeruginosa, that shows allosteric behaviour, and a mutant version of this enzyme has been crystallized in several different crystal forms. All of these have been characterized by X-ray diffraction methods. A 4.5 A resolution data set has been collected on a triclinic crystal. Analysis of the data using the self-rotation function shows that 12 monomers associate to form a particle with cubic 23 point group symmetry.
Marcq
S
S
Institut de Physique, Université de Liège, Belgium.
Diaz-Ruano
A
A
Charlier
P
P
Dideberg
O
O
Tricot
C
C
Piérard
A
A
Stalon
V
V
D046911:Macromolecular Substances; D008961:Models, Structural; D009954:Ornithine Carbamoyltransferase / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D014961:X-Ray Diffraction / Q000379:methods
D046911:Macromolecular Substances; D009954:Ornithine Carbamoyltransferase