title: Effect of heparin derived fractions on the proliferation and protein synthesis of cells in culture.
abstract: We investigated the effect of sulfated oligosaccharides derived from depolymerization of heparin on the proliferation and protein synthesis of smooth muscle cells (SMC), hamster kidney (BHK-21) and lung (V-79) fibroblasts, rat hepatoma cells (FAO) and human promyelocytes (HL-60). BHK-21 and FAO showed the highest sensitivity to heparin; V-79 and HL-60 cells were completely resistant. LMWH (Low Molecular Weight Heparin) (MW 4.5 kD) was as effective as unfractionated heparin in reducing cell proliferation. The oligo-derivative 381/1 (MW 2 kD) was effective only on FAO and BHK-21 cells; oligo-derivative 381/2 (MW 1KD) had a negligible effect. The anti-proliferative effect was associated with an increased secretion of some protein classes. This effect was not present in heparin-resistant cells. In conclusion when the molecular size of heparin derivative is reduced below 2 kD (i.e. the size of a hexasaccharide) the anti-proliferative activity decreases dramatically.
authors.affiliation: Istituto di Patologia Generale, Università di Modena, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Tiozzo
authors.forename: D
authors.initials: D
authors.lastname: Reggiani
authors.forename: M R
authors.initials: MR
authors.lastname: Cingi
authors.forename: P
authors.initials: P
authors.lastname: Bianchini
authors.forename: B
authors.initials: B
authors.lastname: Osima
authors.forename: S
authors.initials: S
authors.lastname: Calandra
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002478:Cells, Cultured; D003871:Dermatan Sulfate / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D009130:Muscle, Smooth / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009844:Oligosaccharides / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification
chemical_list: D009844:Oligosaccharides; D011506:Proteins; D003871:Dermatan Sulfate; D006493:Heparin