[Establishment of a murine ascites hepatoma cell line H 22-F 25/L and its biological characteristics].
Having been passed for 160 generations, a cell line designated as H 22-F 25/L was established from a murine tumor lymphatic metastatic model H 22-F 25 which had been set up in our college. The cell line was in suspension culture with a rapid proliferation and stable growth. The peak time of cell division and proliferation was 48 and 96 hours after culture. In a week, the cell number was increased by 25 times. H 22-F25/L still keeps the features of a poorly differentiated cancer. Its tumor inducing rate (in vivo) was 100% in 615 mice. Lymph node metastasis rate was 50% and pulmonary metastasis rate 10%. H 22-F 25/L is a population of heterogenetic tumor cells including 2 stem cell lines (the model number of chromosomes being 43 in 40% tumor cells and 86 in 32%) and some side lines. The common marker chromosomes M1, M2, M3 and M4 were present in all stem and side lines.
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Dalian Medical College.
D000818:Animals; D005819:Genetic Markers; D008114:Liver Neoplasms, Experimental / Q000235:genetics / Q000473:pathology*; D008207:Lymphatic Metastasis; D051379:Mice; D014407:Tumor Cells, Cultured / Q000648:ultrastructure
D005819:Genetic Markers