Marrow storage techniques: a clinical comparison of refrigeration versus cryopreservation.
Fifty-three patients were evaluated for a comparison of the efficacy, safety, and cost efficiency of bone marrow (BM) transplanted after either refrigeration or cryopreservation. Thirty-eight patients had BM stored at 4 degrees C for an average of 3 days and 15 patients had cryopreserved BM stored for an average of 56 days. The average number of cells harvested was 3.8 x 10(8)/kg. The time to WBC recovery greater than 1 x 10(9)/l was 17 days refrigerated and 23 days for cryopreserved BM. The time to platelet recovery greater than 20 x 10(9)/l was 24 days for refrigeration storage and 51 days for cryopreserved BM. Four of 38 patients with refrigerated vs. 4/15 patients with cryopreserved BM experienced delayed engraftment (p less than 0.05). Refrigeration storage requires no special equipment, is cheaper than and presents a safe and viable alternative to cryopreserved BM in reconstituting hemopoiesis following high-dose chemo-radiotherapy.
Ahmed
T
T
Department of Medicine, New York Medical College, N.Y.
Wuest
D
D
Ciavarella
D
D
Ayello
J
J
Feldman
E J
EJ
Biguzzi
S
S
Gulati
S
S
Hussain
F
F
Mittelman
A
A
Ascensao
J L
JL
D000293:Adolescent; D000328:Adult; D001853:Bone Marrow / Q000502:physiology*; D016026:Bone Marrow Transplantation; D003362:Cost-Benefit Analysis; D015925:Cryopreservation; D005260:Female; D006410:Hematopoiesis; D006689:Hodgkin Disease / Q000097:blood / Q000628:therapy; D006801:Humans; D007938:Leukemia / Q000097:blood / Q000628:therapy; D007958:Leukocyte Count; D008228:Lymphoma, Non-Hodgkin / Q000097:blood / Q000628:therapy; D008297:Male; D010976:Platelet Count; D012034:Refrigeration; D013997:Time Factors; D014021:Tissue Preservation / Q000191:economics / Q000379:methods*