Increase of auto anti-phosphatidylinositol antibodies in plasma of female rats during the appearance of DMBA-induced malignant mammary tumors.
Using a lipid-adapted ELISA, antiphosphatidylinositol (PtdIns) antibodies (Ab) have been found in sera of cancer patients. With the same procedure, we have checked their possible raising in plasma of female rats during the appearance of 7,12-dimethylbenz[a]anthracene (DMBA)-induced mammary tumors. Twenty days after DMBA administration, the mean anti-PtdIns Ab level was already higher than that of control rats (oil). Immunochemical analysis of the anti-PtdIns Ab site showed a rather high Ab avidity (8 x 10(-9) M, at half-displacement) and high specificity for glyceryl phosphate inositol residues. Other phospholipids (PL) were not recognized by the anti-PtdIns Ab induced by malignant cell transformation.
Faiderbe
S
S
Laboratoire d'Immunologie et de Pathologie (CJF 88-13 INSERM), Université de Bordeaux II, France.
Chagnaud
J L
JL
Diolez-Bojda
F
F
Kerdélhué
B
B
Geffard
M
M
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000915:Antibody Affinity; D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology*; D010716:Phosphatidylinositols / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013997:Time Factors
D001323:Autoantibodies; D010716:Phosphatidylinositols; D015127:9,10-Dimethyl-1,2-benzanthracene