Multicenter evaluation of new enzyme-linked immunoassays of follitropin and lutropin in serum or plasma.
Results from a multicenter evaluation of two new enzyme-linked immunosorbent assays [Enzymun-Test for follitropin (FSH) and lutropin (LH)] are presented and compared with results from 11 other commercial immunoassays, radioactive as well as nonradioactive. Enzymun-Test FSH and LH assays are suitable for automated systems and manual applications. The tests were reproducible (CV less than 5%), highly specific, and sensitive enough (less than 0.5 int. unit/L) to measure the hormones directly in almost all patients' samples, except for LH measurements in prepubertal children. We did not find interference by heterophilic antibodies or other factors. A comparison of assays for FSH found very good agreement among all modern two-site assays; competitive immunoassays almost invariably yielded systematically lower results for FSH, probably because of the heterogeneity of the International Reference Preparation (2nd IRP FSH, 78/549). For LH also we found good agreement, with no systematic differences among the various reagents. Guidelines for reference values with the new reagents are given.
Thijssen
J H
JH
Department of Endocrinology, University Hospital, Heidelberg/aan, Utrecht, The Netherlands.
Wood
W G
WG
Kessler
A C
AC
Griesser
H W
HW
Bauer
O
O
Bieglmayer
C
C
Eugui
J
J
Mirabel
J L
JL
Gaessler
H
H
Klinga
K
K
D000328:Adult; D002648:Child; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood*; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood*; D008297:Male; D010907:Pituitary Hormones / Q000378:metabolism; D011247:Pregnancy; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D012016:Reference Values; D015203:Reproducibility of Results
D010907:Pituitary Hormones; D011933:Reagent Kits, Diagnostic; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone