New use of BCG for recombinant vaccines.
BCG, a live attenuated tubercle bacillus, is the most widely used vaccine in the world and is also a useful vaccine vehicle for delivering protective antigens of multiple pathogens. Extrachromosomal and integrative expression vectors carrying the regulatory sequences for major BCG heat-shock proteins have been developed to allow expression of foreign antigens in BCG. These recombinant BCG strains can elicit long-lasting humoral and cellular immune responses to foreign antigens in mice.
Stover
C K
CK
MedImmune Incorporated, Gaithersburg, Maryland 20878.
de la Cruz
V F
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Fuerst
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Burlein
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JE
Benson
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Bennett
L T
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Bansal
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Young
J F
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Lee
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Hatfull
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D000818:Animals; D000941:Antigens / Q000235:genetics* / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D001500:BCG Vaccine / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D005822:Genetic Vectors; D015488:HIV Antigens / Q000235:genetics; D015497:HIV-1 / Q000276:immunology; D006360:Heat-Shock Proteins / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D014614:Vaccines, Synthetic / Q000235:genetics* / Q000276:immunology; D001616:beta-Galactosidase / Q000235:genetics
D000941:Antigens; D000942:Antigens, Bacterial; D000956:Antigens, Viral; D001500:BCG Vaccine; D015488:HIV Antigens; D006360:Heat-Shock Proteins; D014614:Vaccines, Synthetic; D001616:beta-Galactosidase