High performance liquid chromatography of glucose using a post-column reactor of immobilized enzyme followed by electrochemical detection (glucose-LCEC).
A sensitive and selective, reasonably fast method for the determination of glucose content has been developed. A glucose oxidase immobilized column was coupled to a small-size anion exchange column/borate buffer chromatograph. The hydrogen peroxide produced in the enzyme reaction was detected directly by an amperometric detector using a platinum working electrode. The detection limit was 0.03 ppm (1.5 x 10(-7) M, 3 pmol/injection). The linear dynamic range was three orders of magnitude at least. The system was stable and reproducible both in short- and long-term operation. The proposed method is suitable for analysis of complicated matrices of biological samples because of its good selectivity and sensitivity.
Watanabe
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Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan.
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized; D005638:Fruit; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D006029:Glycosuria / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide; D010984:Platinum
D004800:Enzymes, Immobilized; D010984:Platinum; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose