Rel-associated pp40: an inhibitor of the rel family of transcription factors.
The Rel-associated protein pp40 is functionally related to I kappa B, an inhibitor of the transcription factor NF-kappa B. Purified pp40 inhibits the DNA binding activity of the NF-kappa B protein complex (p50:p65 heterodimers), p50:c-Rel heteromers, and c-Rel homodimers. The sequence of the complementary DNA encoding pp40 revealed similarity to the gene encoding MAD-3, a protein with mammalian I kappa B-like activity. Protein sequencing of I kappa B purified from rabbit lung confirmed that MAD-3 encodes a protein similar to I kappa B. The sequence similarity between MAD-3 and pp40 includes a casein kinase II and consensus tyrosine phosphorylation site, as well as five repeats of a sequence found in the human erythrocyte protein ankyrin. These results suggest that rel-related transcription factors, which are capable of cytosolic to nuclear translocation, may be held in the cytosol by interaction with related cytoplasmic anchor molecules.
Davis
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N
Department of Microbiology, University of Texas, Austin 78712.
Ghosh
S
S
Simmons
D L
DL
Tempst
P
P
Liou
H C
HC
Baltimore
D
D
Bose
H R
HR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002642:Chick Embryo; D003001:Cloning, Molecular; D015342:DNA Probes; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D015345:Oligonucleotide Probes; D020572:Oncogene Proteins v-rel; D016366:Open Reading Frames; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D012333:RNA, Messenger / Q000235:genetics; D015682:Retroviridae Proteins, Oncogenic / Q000037:antagonists & inhibitors*; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000037:antagonists & inhibitors*
D015342:DNA Probes; D016328:NF-kappa B; D015345:Oligonucleotide Probes; D020572:Oncogene Proteins v-rel; D010750:Phosphoproteins; D012333:RNA, Messenger; D015682:Retroviridae Proteins, Oncogenic; D014157:Transcription Factors; C070928:Rel-associated protein pp40; D011505:Protein-Tyrosine Kinases