title: Assessment of elastin and collagen contribution to aortic elasticity in conscious dogs.
abstract: The elastic behavior of total elastin (EE) and collagen (EC) and the recruitment of collagen fibers (FC) supporting wall stress at a given transmural pressure level were assessed in seven conscious dogs using descending thoracic aortic pressure (microtransducer) and diameter (sonomicrometer) measurements. Stress-strain relationships values calculated at control and during bolus administration of angiotensin and nitroglycerin enabled quantification of angiotensin and nitroglycerin enabled quantification of elastic moduli of elastin (EE = 4.868 +/- 1.753 x 10(6) dyn/cm2; means +/- SD) and collagen (EC = 1,306 +/- 637 x 10(6) dyn/cm2) according to a biphasic model of elastin and collagen parallel arrangement. The FC was found to be 6.1 +/- 2.6% at a pressure level of 118 +/- 16 mmHg. Values for EE and EC were similar to those reported in in vitro studies and showed scarce variability. This approach provides a quantitative evaluation of elastin and collagen moduli in conscious animals and also permits the evaluation of FC, which may be of interest in studies of connective tissue diseases involving the aortic wall.
authors.affiliation: Research and Teaching Department, Favaloro Foundation, Buenos Aires, Argentina.
authors.forename: R L
authors.initials: RL
authors.lastname: Armentano
authors.forename: J
authors.initials: J
authors.lastname: Levenson
authors.forename: J G
authors.initials: JG
authors.lastname: Barra
authors.forename: E I
authors.initials: EI
authors.lastname: Fischer
authors.forename: G J
authors.initials: GJ
authors.lastname: Breitbart
authors.forename: R H
authors.initials: RH
authors.lastname: Pichel
authors.forename: A
authors.initials: A
authors.lastname: Simon
mesh_terms: D000804:Angiotensin II / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000502:physiology*; D003094:Collagen / Q000502:physiology*; D003243:Consciousness / Q000502:physiology; D004285:Dogs; D004548:Elasticity; D004549:Elastin / Q000502:physiology*; D006439:Hemodynamics / Q000502:physiology; D007275:Injections, Intravenous; D008297:Male; D005996:Nitroglycerin / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D000804:Angiotensin II; D003094:Collagen; D004549:Elastin; D005996:Nitroglycerin