title: Microheterogeneity of urinary albumin and tubular proteinuria in juvenile diabetes mellitus.
abstract: We studied differential urinary albumin excretion by a double one-dimensional gel electrophoresis with decyl sodium sulphate-polyacrylamide gel electrophoresis in the first, and isoelectric focusing in the second dimension in 37 diabetic children and 20 healthy subjects. In addition, total proteins, albumin, beta 2-microglobulin and molecular size distribution of urinary proteins were measured, the latter using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Whilst albuminuria was not significantly different from controls we found an increased microheterogeneity of urinary albumin in 38% of patients. In addition, low molecular weight protein (P less than 0.05) and beta 2-microglobulin excretion (P less than 0.01) were elevated. It is suggested that the appearance of highly heterogenous albumin in the pI range of 5.3-5.9 is the result of a decreased tubular reabsorption.
authors.affiliation: Division of Pediatric Nephrology, University Children's Hospital, Heidelberg, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Ries
authors.forename: K
authors.initials: K
authors.lastname: Schärer
authors.forename: R
authors.initials: R
authors.lastname: Wartha
authors.forename: H
authors.initials: H
authors.lastname: Schmidt
authors.forename: D
authors.initials: D
authors.lastname: Gekle
mesh_terms: D000293:Adolescent; D000328:Adult; D000419:Albuminuria / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000652:urine*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D008970:Molecular Weight; D011507:Proteinuria / Q000652:urine*; D001613:beta 2-Microglobulin / Q000652:urine
chemical_list: D001613:beta 2-Microglobulin