title: Studies on yeast peroxisomal citrate synthase.
abstract: Peroxisomal (nonmitochondrial) citrate synthase (CS2) has been purified from a Saccharomyces cerevisiae strain in which the gene for the mitochondrial citrate synthase (CS1) had been disrupted and no CS1 protein is produced. The enzyme, CS2, the sequence of which had been previously determined from its DNA, behaved differently from CS1 in its purification, kinetics, stability, and binding to the inner surface of mitochondrial inner membranes.
authors.affiliation: Pre-Clinical Science Unit, Veterans Administration Medical Center, Dallas, Texas 75216.
authors.forename: G
authors.initials: G
authors.lastname: Kispal
authors.forename: P A
authors.initials: PA
authors.lastname: Srere
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002950:Citrate (si)-Synthase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008830:Microbodies / Q000201:enzymology*; D008970:Molecular Weight; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D013367:Submitochondrial Particles / Q000378:metabolism; D013552:Swine
chemical_list: D007527:Isoenzymes; D002950:Citrate (si)-Synthase