Characterization of a novel type of chain-terminator Gal beta 1-6Gal beta 1-4)GlcNAc in an oligosaccharide related to N-glycosylated protein glycans isolated from GM1 the urine of patients with gangliosidosis.
Two new oligosaccharides were isolated from the urine of a patient with GM1 gangliosidosis. Final purification of the oligosaccharides was accomplished by capillary supercritical fluid chromatography. Structural analysis was by chemical analysis, chemical-ionization mass spectrometry and 400-MHz 1H-NMR spectroscopy, leading to two primary structures. The first is derived from a classical triantennary N-acetyllactosamine-type glycan: Gal beta 1-4GlcNAc beta 1-4(Gal beta 1-4GlcNAc beta 1-2)Man alpha 1-3Man beta 1-4GlcNAc. The second is unusual with a terminal disaccharide Gal beta 1-6Gal, which had not yet been described for glycans of the N-acetyllactosamine type: Gal beta 1-6Gal beta 1-4GlcNAc beta 1-2Man alpha 1-6Man beta 1-4GlcNAc.
Michalski
J C
JC
Laboratoire de Chimie Biologique, Unité Mixte de Recherche, Université des Sciences et Techniques de Lille Flandres-Artois, France.
Lemoine
J
J
Wieruszeski
J M
JM
Fournet
B
B
Montreuil
J
J
Strecker
G
G
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002854:Chromatography, Paper; D005677:G(M1) Ganglioside / Q000302:isolation & purification / Q000652:urine*; D005733:Gangliosidoses / Q000652:urine*; D006031:Glycosylation; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification / Q000652:urine*; D011509:Proteoglycans / Q000302:isolation & purification / Q000652:urine*
D009844:Oligosaccharides; D011509:Proteoglycans; D005677:G(M1) Ganglioside