Microheterogeneity of the alpha subunits of G proteins in bovine brain clathrin coated vesicles.
Clathrin coated vesicles (CV) from bovine brain express the various alpha subunits of guanine nucleotide regulatory proteins (G-proteins): Go, Gi 1, 2, 3 and Gs. Western blot analysis using two different antisera revealed the presence of the 39-kDa Go alpha subunit in CV. Antisera RM/1, in turn, demonstrated the expression of the short (45 kDa) and long (52 kDa) form variants of the Gs alpha subunit. The analysis demonstrates that the heterogeneity of alpha subunits of G-proteins in bovine brain tissue is also reflected in the CV transport organelles.
Silva
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Department of Pharmacology, Universidad Central del Caribe, School of Medicine, Bayamón, Puerto Rico 00621-6032.
D000818:Animals; D015153:Blotting, Western; D001923:Brain Chemistry; D002417:Cattle; D003034:Coated Pits, Cell-Membrane / Q000737:chemistry*; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000502:physiology
D019204:GTP-Binding Proteins