Metabolism of cyanide by Phanerochaete chrysosporium.
The oxidation of veratryl alcohol (3,4-dimethoxybenzyl alcohol) by lignin peroxidase H2 (LiP H2) from the white rot fungus Phanerochaete chrysosporium was strongly inhibited by sodium cyanide. The I50 was estimated to be about 2-3 microM. In contrast, sodium cyanide binds to the native enzyme with an apparent sodium cyanide dissociation constant Kd of about 10 microM. Inhibition of the veratryl alcohol oxidase activity of LiP H2 by cyanide was reversible. Ligninolytic cultures of P. chrysosporium mineralized cyanide at a rate that was proportional to the concentration of cyanide to 2 mM. The N-tert-butyl-alpha-phenylnitrone-cyanyl radical adduct was observed by ESR spin trapping upon incubation of LiP H2 with H2O2 and sodium cyanide. The identity of the spin adduct was confirmed using 13C-labeled cyanide. Six-day-old cultures of the fungus were more tolerant to sodium cyanide toxicity than spores. Toxicity measurements were based on the effect of sodium cyanide on respiration of the fungus as determined by the metabolism of [14C]glucose to [14C]CO2. We propose that this tolerance of the mature fungus was due to its ability to mineralize cyanide and that this fungus might be effective in treating environmental pollution sites contaminated with cyanide.
Shah
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Biotechnology Center, Utah State University, Logan 84322-4700.
Grover
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Aust
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D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism; D001487:Basidiomycota / Q000187:drug effects / Q000378:metabolism*; D001592:Benzyl Alcohols / Q000378:metabolism; D001673:Biodegradation, Environmental; D002245:Carbon Dioxide / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D010544:Peroxidases / Q000037:antagonists & inhibitors / Q000378:metabolism; D012966:Sodium Cyanide / Q000378:metabolism* / Q000633:toxicity
D001592:Benzyl Alcohols; D002245:Carbon Dioxide; D006861:Hydrogen Peroxide; D000429:Alcohol Oxidoreductases; C057990:veratryl alcohol oxidase; D010544:Peroxidases; C042858:lignin peroxidase; D005947:Glucose; C042197:veratryl alcohol; D012966:Sodium Cyanide