title: Cloning and expression of genomic DNA sequences coding for putative erythrocyte membrane-associated antigens of Plasmodium falciparum.
abstract: Genomic DNA fragments of Plasmodium falciparum generated by mung bean nuclease digestion were cloned in the lambda expression vector lambda JK2. The resulting library was screened with a rabbit antiserum raised against purified membranes of P. falciparum-infected erythrocytes and with a serum pool from immune humans from an endemic area of Liberia. Positive clones were rescreened with a series of human and monkey sera. Twelve selected clones were analysed in detail. Four of them corresponded to already described membrane-associated P. falciparum antigens. The other positive clones contained inserts which, according to the nucleotide sequence, Southern blot analysis and immunological characteristics, correspond to so far unknown antigens.
authors.affiliation: Institut für Genetik Universität zu Köln, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Kun
authors.forename: J
authors.initials: J
authors.lastname: Hesselbach
authors.forename: M
authors.initials: M
authors.lastname: Schreiber
authors.forename: A
authors.initials: A
authors.lastname: Scherf
authors.forename: J
authors.initials: J
authors.lastname: Gysin
authors.forename: D
authors.initials: D
authors.lastname: Mattei
authors.forename: L
authors.initials: L
authors.lastname: Pereira da Silva
authors.forename: B
authors.initials: B
authors.lastname: Müller-Hill
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000235:genetics*; D004910:Erythrocyte Membrane / Q000276:immunology / Q000469:parasitology; D006801:Humans; D008288:Malaria / Q000276:immunology; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics* / Q000276:immunology; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins