Human placental sialidase complex: characterization of the 60 kDa protein that cross-reacts with anti-saposin antibodies.
Sialidase isolated from human placenta is associated with several proteins including acid beta-galactosidase, carboxypeptidase, N-acetyl-alpha-galactosaminidase, and others. These proteins are thought to form an aggregated complex during isolation of sialidase. One of the proteins of 60 kDa was recently identified by Potier et al. (Biochem. Biophys. Res. Comm. 173, 449-456, 1990) as a sialidase protein: this protein also cross-reacted with anti-prosaposin antibodies. We have isolated this protein and from the following evidence identified it as a heavy chain component of immunoglobulin G and not sialidase or a derivative of prosaposin. On gel filtration HPLC, sialidase activity and the 60 kDa protein were clearly separated from one another. The 60 kDa protein cross-reacted not only with antibodies raised against human saposins A, C, and D, but also with second antibody (goat anti-rabbit immunoglobulin G antibody) alone. This 60 kDa protein strongly cross-reacted with anti-human immunoglobulin G antibodies. The sequence of the initial 15 amino acids from the N-terminus of the 60 kDa protein was identical to the sequence of an immunoglobulin G heavy chain protein Tie (gamma 1).
Hiraiwa
M
M
Department of Neurosciences, Center for Molecular Genetics, University of California, San Diego, La Jolla 92093.
Uda
Y
Y
Tsuji
S
S
Miyatake
T
T
Martin
B M
BM
Tayama
M
M
O'Brien
J S
JS
Kishimoto
Y
Y
D000595:Amino Acid Sequence; D000936:Antigen-Antibody Complex; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000276:immunology* / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009439:Neuraminidase / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D011506:Proteins / Q000276:immunology*; D049231:Saposins; D012689:Sequence Homology, Nucleic Acid
D000936:Antigen-Antibody Complex; D006023:Glycoproteins; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D011506:Proteins; D049231:Saposins; D009439:Neuraminidase