Isolated MTAL cells produce an inhibitor of ouabain-sensitive oxygen consumption.
Dispersed medullary thick ascending limb (MTAL) cells of the rabbit kidney produce an inhibitor of transport-related oxygen consumption when incubated at 37 degrees C. Appearance of this inhibitor is enhanced by incubation with arachidonic acid. Its appearance can be prevented by either 10(-6) M nordihydroguaiaretic acid or 10(-4) M indomethacin, but not by 10(-6) M indomethacin. These results suggest that the transport inhibitor is an eicosanoid produced by a pathway other than that catalyzed by cyclooxygenase. It inhibits ouabain-sensitive respiration in intact cells treated with amphotericin, suggesting direct inhibition of the Na(+)-K(+)-ATPase.
Cantley
L G
LG
Department of Medicine, Beth Israel Hospital, Boston, Massachusetts 02215.
Fuhro
R
R
Silva
P
P
D000666:Amphotericin B / Q000494:pharmacology; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002469:Cell Separation; D003470:Culture Media; D004573:Electrolytes / Q000378:metabolism; D007213:Indomethacin / Q000494:pharmacology; D008138:Loop of Henle / Q000166:cytology / Q000378:metabolism*; D009637:Masoprocol / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects*; D013997:Time Factors
D001095:Arachidonic Acids; D003470:Culture Media; D004573:Electrolytes; D016718:Arachidonic Acid; D010042:Ouabain; D009637:Masoprocol; D000666:Amphotericin B; D007213:Indomethacin