title: Progesterone production in cultured human granulosa cells: correlation with follicular fluid hormone levels.
abstract: OBJECTIVE
  To examine the progesterone (P) production by cultured granulosa cells and the hormonal content in the follicular fluid (FF) of ovarian-hyperstimulated women.
  
  
  METHODS
  Retrospective.
  
  
  METHODS
  Private Fertility Clinic and National Research Institute.
  
  
  METHODS
  Eighteen patients undergoing in vitro fertilization or gamete intrafallopian transfer programs.
  
  
  RESULTS
  Progesterone levels Measured in the culture medium of granulosa cells decreased sixfold with culture time. Human luteinizing hormone (LH) increased P production only when basal P production was less than 1 microgram/mL. Granulosa cell P production in culture was negatively correlated with FF LH-human chorionic gonadotropin (hCG) levels. Follicular fluid follicle-stimulating hormone (FSH) levels were positively correlated with FF P and 17 beta-estradiol (E2) concentrations. Similar results were found between FF LH (hCG) and E2 levels, but there was no relationship between FF LH (hCG) and FF P values.
  
  
  CONCLUSIONS
  The high dose of hCG administered during gonadotropin treatment could induce a decrease in the in vitro granulosa cell P production.
authors.affiliation: Instituto de Biologia y Medicina Experimental, Buenos Aires, Argentina.
authors.forename: L B
authors.initials: LB
authors.lastname: Dain
authors.forename: P
authors.initials: P
authors.lastname: Stein
authors.forename: A R
authors.initials: AR
authors.lastname: Krimer
authors.forename: R H
authors.initials: RH
authors.lastname: Asch
authors.forename: E P
authors.initials: EP
authors.lastname: de Fried
authors.forename: E H
authors.initials: EH
authors.lastname: Charreau
authors.forename: M
authors.initials: M
authors.lastname: Tesone
mesh_terms: D002478:Cells, Cultured; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000502:physiology; D015181:Gamete Intrafallopian Transfer; D006107:Granulosa Cells / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007986:Luteinizing Hormone / Q000494:pharmacology* / Q000502:physiology; D006080:Ovarian Follicle / Q000502:physiology*; D011374:Progesterone / Q000096:biosynthesis*; D012044:Regression Analysis
chemical_list: D011374:Progesterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone