Comparison of pertussis toxin (PT)-neutralizing activities and mouse-protective activities of anti-PT mouse monoclonal antibodies.
An assay of neutralizing activity against CHO cell-clustering activity, one of the most common assays for anti-pertussis toxin antibodies, did not correlate well with an assay of the protective activities of monoclonal antibodies. A better correlation between neutralization of leukocytosis-promoting or islet-activating activity and mouse protection against aerosol challenge was seen.
Sato
H
H
Department of Applied Immunology, National Institute of Health, Tokyo, Japan.
Sato
Y
Y
Ohishi
I
I
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D006224:Cricetinae; D007964:Leukocytosis / Q000139:chemically induced; D051379:Mice; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella / Q000276:immunology* / Q000633:toxicity; D014917:Whooping Cough / Q000517:prevention & control*
D000911:Antibodies, Monoclonal; D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D037342:Pertussis Toxin