title: Amino acid sequence of the blue copper protein rusticyanin from Thiobacillus ferrooxidans.
abstract: Rusticyanin is a small blue copper protein isolated from Thiobacillus ferrooxidans. The amino acid sequence of the rusticyanin has been determined by the structural characterization of tryptic and endoproteinase Asp-N peptides with use of amino terminal microsequencing, fast atom bombardment mass spectrometry, and electrospray triple-quadrupole mass spectrometry techniques. Amino acid analysis, carboxy-terminal sequence analysis, and circular dichroism spectroscopy were also performed on the protein. Amino acid sequence identity among rusticyanin and six other small blue copper proteins is apparent only in the limited C-terminal region of each protein bearing three of the four putative copper ligands. A structural model of the rusticyanin is proposed where the protein is principally a beta-barrel comprised of six strands. This model is consistent with the circular dichroism data and computational predictions of the secondary structure of rusticyanin. A feature of the model is the hypothesis that Asp 73 may serve as a fourth copper ligand.
authors.affiliation: Division of Immunology, Beckman Research Institute of the City of Hope, Duarte, California 91010.
authors.forename: M
authors.initials: M
authors.lastname: Ronk
authors.forename: J E
authors.initials: JE
authors.lastname: Shively
authors.forename: E A
authors.initials: EA
authors.lastname: Shute
authors.forename: R C
authors.initials: RC
authors.lastname: Blake
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001400:Azurin / Q000031:analogs & derivatives* / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry*; D002942:Circular Dichroism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry; D011487:Protein Conformation; D016415:Sequence Alignment; D013855:Thiobacillus / Q000032:analysis*
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D010455:Peptides; C012543:rusticyanin; D001400:Azurin