[Increase by FSH hormone and depression by testosterone of the diffusional coupling between Sertoli cells from immature rat testis in primary culture].
Previous studies have suggested that FSH promotes the intercellular coupling of Sertoli cells from immature rat testis in primary culture ([1], [2]). In order to test this hypothesis, we have investigated the diffusional coupling between Sertoli cells in primary culture with the FRAP technique. The coupling is low in unstimulated cells but increases in the presence of FSH. This effect is not reversed by returning to the control medium. Testosterone decreases this coupling, an effect which is reversed by a new exposure to FSH. Taken together these data show that FSH initiates diffusional coupling in Sertoli cells and that testosterone antagonizes this effect.
Pluciennik
F
F
Unité C.N.R.S. n 290, Faculté des Sciences, Poitiers.
Joffre
M
M
Délèze
J
J
D000818:Animals; D002478:Cells, Cultured; D004058:Diffusion / Q000187:drug effects; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012708:Sertoli Cells / Q000502:physiology*; D013050:Spectrometry, Fluorescence; D013739:Testosterone / Q000494:pharmacology*
D013739:Testosterone; D005640:Follicle Stimulating Hormone
Accroissement par l'hormone FSH et dépression par la testostérone du couplage diffusionnel existant entre les cellules de Sertoli du testicule de rat immature en culture primaire.