Isolation and characterization of catabolite repression control mutants of Pseudomonas aeruginosa PAO.
Independently controlled, inducible, catabolic genes in Pseudomonas aeruginosa are subject to strong catabolite repression control by intermediates of the tricarboxylic acid cycle. Mutants which exhibited a pleiotropic loss of catabolite repression control of multiple pathways were isolated. The mutations mapped in the 11-min region of the P. aeruginosa chromosome near argB and pyrE and were designated crc. Crc- mutants no longer showed repression of mannitol and glucose transport, glucose-6-phosphate dehydrogenase, glucokinase, Entner-Doudoroff dehydratase and aldolase, and amidase when grown in the presence of succinate plus an inducer. These activities were not expressed constitutively in Crc- mutants but exhibited wild-type inducible expression.
Wolff
J A
JA
Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond 23298.
MacGregor
C H
CH
Eisenberg
R C
RC
Phibbs
P V
PV
D000577:Amides / Q000378:metabolism; D000581:Amidohydrolases / Q000096:biosynthesis / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001692:Biological Transport; D002952:Citric Acid Cycle; D003227:Conjugation, Genetic; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism; D008353:Mannitol / Q000378:metabolism; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D012097:Repressor Proteins / Q000235:genetics*; D013386:Succinates / Q000378:metabolism; D014161:Transduction, Genetic
D000577:Amides; D001426:Bacterial Proteins; D012097:Repressor Proteins; D013386:Succinates; D008353:Mannitol; C013083:lactamide; D000581:Amidohydrolases; D005947:Glucose