The effects of recombinant human interferon-gamma on a panel of human bladder cancer cell lines.
We have examined the Major Histocompatibility Complex class II antigen inducing capabilities of recombinant human interferon-gamma, on a panel of human transitional cell carcinoma lines which have been raised from original tumours of varying histological grades: RT4 (grade 1), RT112 (grade 2) and MGH-U1 (grade 3). Cells were examined for class II antigens using an indirect immunofluorescent staining method and analysed on a fluorescence activated cell sorter. Twenty percent of RT4 cells constitutively expressed class II antigen. Both RT112 and MGH-U1 were repeatedly found to be negative for this antigen prior to treatment with interferon-gamma. Following treatment with interferon-gamma all three lines showed an increase in class II antigen expression, which was consistently dependent on both the length of incubation and concentration of interferon-gamma. A differential susceptibility was found amongst the three cell lines which may relate to the histological grade of the parent tumor.
Hawkyard
S
S
Department of Surgery, Western General Hospital, Edinburgh, United Kingdom.
James
K
K
Prescott
S
S
Jackson
A M
AM
Ritchie
A W
AW
Smyth
J F
JF
Chisholm
G D
GD
D002295:Carcinoma, Transitional Cell / Q000276:immunology*; D002460:Cell Line; D002469:Cell Separation; D004354:Drug Screening Assays, Antitumor; D005455:Fluorescent Antibody Technique; D006684:HLA-DR Antigens / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D011994:Recombinant Proteins; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
D006684:HLA-DR Antigens; D011994:Recombinant Proteins; D007371:Interferon-gamma