Improved method for coliform verification.
Modification of a method for coliform verification presented in Standard Methods for the Examination of Water and Wastewater is described. Modification of the method, which is based on beta-galactosidase production, involves incorporation of a lactose operon inducer in medium upon which presumptive coliform isolates are cultured prior to beta-galactosidase assay.
Diehl
J D
JD
Biology Department, Lycoming College, Williamsport, Pennsylvania 17701.
D001431:Bacteriological Techniques; D004755:Enterobacteriaceae / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D007763:Lac Operon / Q000187:drug effects; D014871:Water Microbiology; D014881:Water Supply / Q000032:analysis; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics
D007544:Isopropyl Thiogalactoside; D001616:beta-Galactosidase