Effects of protopine on blood platelet aggregation. III. Effect of protopine on the metabolic system of arachidonic acid in platelets.
The mode of action of protopine on blood platelet aggregation was investigated in the metabolic system of arachidonic acid and in liberation of platelet activating factor using in vitro experimental models. Protopine inhibited the releases of arachidonic acid and platelet activating factor from platelet membrane phospholipids. Protopine also inhibited the conversion of prostaglandin G2 to thromboxane A2, as well as carboxyheptyl imidazole, a thromboxane synthetase inhibitor. These results indicated that protopine functions both as a phospholipase inhibitor and a thromboxane synthetase inhibitor. It is expected that protopine can be applied for treatment of thrombosis as an antiplatelet drug.
Shiomoto
H
H
Faculty of Pharmaceutical Sciences, Kinki University, Osaka, Japan.
Matsuda
H
H
Kubo
M
M
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood*; D053119:Benzophenanthridines; D001600:Berberine Alkaloids; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011817:Rabbits
D000470:Alkaloids; D001095:Arachidonic Acids; D053119:Benzophenanthridines; D001600:Berberine Alkaloids; D010975:Platelet Aggregation Inhibitors; D016718:Arachidonic Acid; C009093:protopine