Inhibitory effects of radical scavengers on diacylglycerol-promoted transformation in BALB/3T3 cells.
To discover the relationship between the activation of protein kinase C and the generation of reactive oxygen in the tumor promotion process, we investigated the effects of radical scavengers on diacylglycerol-promoted transformation in BALB/3T3 cells. Superoxide dismutase (SOD) showed inhibitory effects on both 1-oleoyl-2-acetylglycerol (OAG)-promoted and diolein-promoted transformation. Catalase (CT) suppressed the promoting effects of diolein by up to 70%. Mannitol (MT), a hydroxyl radical (.OH) scavenger, inhibited diacylglycerol-promoted transformation dose-dependently. These results suggest that activation of protein kinase C alone is insufficient and that generation of reactive oxygen accompanied by activation of the enzyme is essential to the promotion process in BALB/3T3 cells.
Semba
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M
Laboratory of Cell Toxicology, Hatano Research Institute, Food and Drug Safety Center, Kanagawa, Japan.
Inui
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N
D000818:Animals; D002374:Catalase / Q000494:pharmacology; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced* / Q000187:drug effects; D002478:Cells, Cultured; D004075:Diglycerides / Q000494:pharmacology*; D016166:Free Radical Scavengers; D005609:Free Radicals; D008353:Mannitol / Q000494:pharmacology; D008748:Methylcholanthrene / Q000494:pharmacology; D051379:Mice; D010100:Oxygen / Q000378:metabolism; D013482:Superoxide Dismutase / Q000494:pharmacology
D004075:Diglycerides; D016166:Free Radical Scavengers; D005609:Free Radicals; D008353:Mannitol; D008748:Methylcholanthrene; C038237:1-oleoyl-2-acetylglycerol; D002374:Catalase; D013482:Superoxide Dismutase; D010100:Oxygen; C013965:diolein