Purification to homogeneity of bovine prolactin by high-performance ion-exchange chromatography.
Homogeneous bovine prolactin (bPRL) has been obtained using a procedure based on high-performance anion-exchange chromatography. The procedure enables up to 6 mg of 99.4% pure bPRL to be obtained per hour, with a recovery of 32.4%. The purity of the protein was checked by N-terminal sequencing and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The highly purified bPRL obtained with this method is suitable for complete structural and immunochemical studies.
Berrini
A
A
Istituto di Fisiologia Veterinaria e Biochimica, Università di Milano, Italy.
Borromeo
V
V
Secchi
C
C
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D010902:Pituitary Gland / Q000737:chemistry; D011388:Prolactin / Q000302:isolation & purification*
D011388:Prolactin