Rapid and sensitive assay for the phytotoxin rhizobitoxine.
Rhizobitoxine is a phytotoxin synthesized by some strains of the legume symbiont genus Bradyrhizobium and the plant pathogen Pseudomonas andropogonis. We demonstrate here a new enzymatic assay which is 100-fold more sensitive than previous assays and can detect as little as 1.0 pmol of rhizobitoxine. The assay is based on the inhibition of Salmonella typhimurium beta-cystathionase by rhizobitoxine. Interestingly, beta-cystathionase from Bradyrhizobium japonicum is insensitive to rhizobitoxine at concentrations lower than 75 microM.
Ruan
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Department of Agronomy, Ohio State University, Columbus 43210-1002.
Peters
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D008190:Lyases / Q000037:antagonists & inhibitors; D011412:Propanolamines / Q000032:analysis* / Q000494:pharmacology; D011549:Pseudomonas / Q000032:analysis*; D012230:Rhizobiaceae / Q000032:analysis*; D012486:Salmonella typhimurium / Q000201:enzymology
D011412:Propanolamines; D008190:Lyases; C019965:cystathionine beta-lyase; C041618:rhizobitoxine