Directions in gene therapy.
Retroviral mediated gene transfer has proven to be an effective method of transferring genetic material into many different mammalian cells. Presented in this short review is a history of retroviral-mediated gene transfer as it relates to hematopoietic- and blood-derived cells. Gene transfer into mouse, primate, and fetal sheep and primate hematopoietic stem cells is discussed. Gene transfer into lymphocytes, in particular tumor-infiltrating lymphocytes, is also addressed. The use of such lymphocytes in ongoing and submitted clinical protocols utilizing retroviral-mediated gene transfer are presented.
Moen
R C
RC
Genetic Therapy, Inc., Gaithersburg, Maryland.
D000243:Adenosine Deaminase / Q000172:deficiency* / Q000235:genetics; D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods; D016332:Fetal Tissue Transplantation / Q000379:methods; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D051379:Mice; D011323:Primates; D012190:Retroviridae / Q000235:genetics; D014162:Transfection
D000243:Adenosine Deaminase