title: Inhibitory effect of tachyplesin I on the proliferation of human immunodeficiency virus in vitro.
abstract: An antimicrobial peptide, tachyplesin I, isolated from hemocytes of the Japanese horseshoe crab (Tachypleus tridentatus) was examined for its inhibitory effects on human immunodeficiency virus (HIV) infection in vitro. At a concentration of 7.5 micrograms/ml, tachyplesin I suppressed the development of cytopathic effects (CPE) by more than 70% in MT-4 cells infected with HIV (lymphadenopathy-associated virus). This inhibitory effect was observed only when the drug was added during the adsorption period of the virus to the cells. In cocultures of MOLT-4 and persistently HIV-infected cells (MOLT-4/HIV), tachyplesin I at the same concentration completely inhibited multinucleated giant cell formation. Infectivity of HIV was reduced by 10(-2.5) in medium free from fetal calf serum containing tachyplesin I at a concentration of 200 micrograms/ml. Tachyplesin I did not show any inhibitory effect on reverse transcriptase activity of HIV at concentrations of 9-80 micrograms/ml at which tachyplesin I inhibited HIV infection. These results suggest that the anti-HIV action of tachyplesin I was due to the inhibition of virus adsorption.
authors.affiliation: Osaka Prefectural Institute of Public Health, Osaka, City University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Morimoto
authors.forename: H
authors.initials: H
authors.lastname: Mori
authors.forename: T
authors.initials: T
authors.lastname: Otake
authors.forename: N
authors.initials: N
authors.lastname: Ueba
authors.forename: N
authors.initials: N
authors.lastname: Kunita
authors.forename: M
authors.initials: M
authors.lastname: Niwa
authors.forename: T
authors.initials: T
authors.lastname: Murakami
authors.forename: S
authors.initials: S
authors.lastname: Iwanaga
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D002478:Cells, Cultured; D004268:DNA-Binding Proteins; D006678:HIV / Q000472:pathogenicity / Q000502:physiology*; D006434:Hemocytes / Q000382:microbiology; D010455:Peptides / Q000494:pharmacology*; D010456:Peptides, Cyclic; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D004268:DNA-Binding Proteins; D010455:Peptides; D010456:Peptides, Cyclic; C057636:tachyplesin peptide, Tachypleus tridentatus