The effect of class II gene transfection on the tumourigenicity of the H-2K-negative mouse leukaemia cell line K36.16.
There is much evidence to suggest that potentially immunogenic tumour cells can escape cytolytic immune destruction by loss of class I antigen expression. Many tumours are allele-specific class I negative and, in murine systems, reconstitution of class I expression by gene transfection leads to an increase in tumour immunogenicity. In many systems where mice have rejected class I transfected tumour cells they are also immune to a subsequent challenge with the untransfected parent tumour. In this study we have examined the effect of stable class II antigen expression (induced by gene transfection) on a class I loss mutant (H-2Kk negative) murine cell line, K36.16. We show that H-2Ek expression is more effective at increasing tumour immunogenicity than the reconstitution of H-2Kk expression in these cells. This suggests that the induction of class II antigen expression on tumour cells may provide an effective way of enhancing tumour-specific immune responses in vivo.
James
R F
RF
Department of Surgery, Leicester University, U.K.
Edwards
S
S
Hui
K M
KM
Bassett
P D
PD
Grosveld
F
F
D000818:Animals; D002460:Cell Line; D003602:Cytotoxicity, Immunologic / Q000235:genetics; D005802:Genes, MHC Class II / Q000276:immunology*; D006183:H-2 Antigens / Q000032:analysis*; D007109:Immunity / Q000235:genetics; D007942:Leukemia, Experimental / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008806:Mice, Inbred AKR; D014162:Transfection / Q000276:immunology*
D006183:H-2 Antigens; C068318:H-2E(k) antigen; C024755:H-2K(K) antigen