Modulation of cell growth and differentiation induced by prostaglandin D2 in the glioma cell line C6.
Prostaglandins are produced mainly from arachidonic in various normal and neoplastic tissues and are well-known to be involved in the regulation of tumor cell proliferation, differentiation and metastasis. Prostaglandin D2 is cytotoxic to several human tumor cell lines, has antitumoral activity in vitro and is reported to be antimetastatic. However, the effects of prostaglandin D2 on tumoral cell differentiation have not been clarified. This paper studies the effects of prostaglandin D2 on C6 rat glioma cell differentiation and proliferation. Prostaglandin D2 administration in the culture medium is observed to alter cell morphology and cytoskeleton filament patterns. These alterations suggest a cell maturation, and correlate with a drop in proliferating capacity. The antitumoral activity of prostaglandin D2 involves a biomodulation of cell parameters which would affect neoplastic processes.
Conde
B
B
Department of Morphology (Tissue Culture), School of Medical, University of Zaragoza, Spain.
Tejedor
M
M
Sinues
E
E
Alcala
A
A
D000818:Animals; D000970:Antineoplastic Agents; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure; D004354:Drug Screening Assays, Antitumor; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D005910:Glioma; D015230:Prostaglandin D2 / Q000494:pharmacology*; D051381:Rats; D013861:Thiocyanates; D014746:Vimentin / Q000032:analysis
D000970:Antineoplastic Agents; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005904:Glial Fibrillary Acidic Protein; D013861:Thiocyanates; D014746:Vimentin; D016650:Fluorescein-5-isothiocyanate; D015230:Prostaglandin D2