title: Formation of glycoprotein degrading enzymes by Bacteroides fragilis.
abstract: Bacteroides fragilis NCDO 2217 produced a wide range of cell-associated hydrolytic enzymes (neuraminidase, alpha-fucosidase, alpha-N-acetylgalactosaminidase, beta-galactosidase, beta-N-acetylglucosaminidase) that could potentially degrade the carbohydrate moieties of mucin, a complex glycoprotein. The type of substrate used for growth markedly influenced their formation in batch cultures. Synthesis of neuraminidase, alpha-fucosidase, alpha-N-acetylgalactosaminidase and to a lesser extent, beta-N-acetylglucosaminidase, was inversely related to growth rate in continuous cultures (D = 0.03 h-1-0.23 h-1) in which porcine gastric mucin provided the sole source of carbon and nitrogen.
authors.affiliation: MRC Dunn Clinical Nutrition Centre, Cambridge, U.K.
authors.forename: G T
authors.initials: GT
authors.lastname: Macfarlane
authors.forename: G R
authors.initials: GR
authors.lastname: Gibson
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D001441:Bacteroides fragilis / Q000201:enzymology* / Q000254:growth & development; D050260:Carbohydrate Metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006596:Hexosaminidases / Q000378:metabolism; D007700:Kinetics; D009077:Mucins / Q000302:isolation & purification / Q000378:metabolism*; D009439:Neuraminidase / Q000378:metabolism; D013552:Swine; D005644:alpha-L-Fucosidase / Q000378:metabolism; D048809:alpha-N-Acetylgalactosaminidase; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D009077:Mucins; D006026:Glycoside Hydrolases; D006596:Hexosaminidases; D009439:Neuraminidase; D001616:beta-Galactosidase; D048809:alpha-N-Acetylgalactosaminidase; D005644:alpha-L-Fucosidase; D000118:Acetylglucosaminidase