Detection of human spermatid-specific transcripts in peripheral blood lymphocytes of males and females.
We describe the detection of ectopic ("illegitimate") transcripts of the proacrosin and protamine 2 genes, which are specific for human spermatogenesis, in non-cultured peripheral blood lymphocytes. After specifically-primed reverse transcription of total lymphocyte RNA, these rare transcripts can be directly visualised after two rounds of polymerase chain reaction with nested primers. Sequence and restriction analyses of the corresponding fragments have confirmed that transcripts of proacrosin and protamine 2 are present in the lymphocytes not only of males, but also of adult females.
Slomski
R
R
Institut für Humangenetik der Universität, Göttingen, Federal Republic of Germany.
Schloesser
M
M
Chlebowska
H
H
Reiss
J
J
Engel
W
W
D000176:Acrosin / Q000097:blood* / Q000235:genetics; D001483:Base Sequence; D004792:Enzyme Precursors / Q000097:blood* / Q000235:genetics; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011479:Protamines / Q000097:blood* / Q000235:genetics; D012333:RNA, Messenger / Q000097:blood*; D013087:Spermatids / Q000378:metabolism*; D014158:Transcription, Genetic
D004792:Enzyme Precursors; D011479:Protamines; D012333:RNA, Messenger; C068621:protamine 2; C022438:proacrosin; D000176:Acrosin