Analysis of paralytic shellfish poisons by capillary electrophoresis.
A capillary electrophoresis (CE) method with UV detection is described for the separation and determination of underivatized toxins associated with paralytic shellfish poisoning (PSP). Confirmation of the electrophoretic peaks was facilitated by mass spectrometric (MS) detection using an ionspray CE-MS interface and by high-performance liquid chromatography with fluorescence detection. The determination of PSP toxins, such as saxitoxin and neosaxitoxin, in toxic dinoflagellates and scallops is demonstrated and comparisons are made with existing techniques.
Thibault
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P
Institute for Murine Biosciences, National Research Council of Canada, Halifax, Nova Scotia.
Pleasance
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S
Laycock
M V
MV
D000818:Animals; D002197:Capillary Action; D000458:Cyanobacteria / Q000032:analysis; D004141:Dinoflagellida / Q000032:analysis; D004586:Electrophoresis / Q000379:methods*; D008099:Liver / Q000737:chemistry; D008387:Marine Toxins / Q000032:analysis*; D008974:Mollusca / Q000032:analysis; D012530:Saxitoxin / Q000031:analogs & derivatives / Q000032:analysis; D012758:Shellfish
D008387:Marine Toxins; D012530:Saxitoxin; C034902:neosaxitoxin