Purification, crystallization and preliminary crystallographic analysis of porcine aldose reductase.
Large crystals of porcine aldose reductase have been grown from polyethylene glycol solutions. The crystals are triclinic, space-group P1, with a = 81.3 A, b = 85.9 A, c = 56.6 A, alpha = 102.3 degrees, beta = 103.3 degrees and gamma = 79.0 degrees. The crystals grow within ten days to dimensions of 0.6 mm x 0.4 mm x 0.2 mm and diffract to at least 2.5 A. There are four molecules in the unit cell related by a set of three mutually perpendicular non-crystallographic 2-fold axes.
el-Kabbani
O
O
University of Alabama, Center for Macromolecular Crystallography, Birmingham 35294.
Narayana
S V
SV
Babu
Y S
YS
Moore
K M
KM
Flynn
T G
TG
Petrash
J M
JM
Westbrook
E M
EM
DeLucas
L J
LJ
Bugg
C E
CE
D000449:Aldehyde Reductase / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D011487:Protein Conformation; D013552:Swine; D014961:X-Ray Diffraction
D000449:Aldehyde Reductase