Chromatosomes are not produced from Tetrahymena chromatin by micrococcal nuclease digestion.
The subnucleosomal organization of Tetrahymena chromatin, which has an unusual H1 histone, was investigated by NaCl extraction and micrococcal nuclease digestion of nuclei. It was found that Tetrahymena histone H1 is extracted with 0.35 M NaCl, whereas bovine thyroid H1 is not. Micrococcal nuclease digestion of Tetrahymena nuclei did not yield chromatosomes as a stable intermediate, whereas digestion of bovine thyroid nuclei did.
Suda
M
M
Institute of Endocrinology, Gunma University, Japan.
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000648:ultrastructure; D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000302:isolation & purification; D008836:Micrococcal Nuclease; D009687:Nuclear Proteins / Q000302:isolation & purification*; D013769:Tetrahymena pyriformis / Q000648:ultrastructure*; D013961:Thyroid Gland / Q000648:ultrastructure
D002843:Chromatin; D006657:Histones; D009687:Nuclear Proteins; D004247:DNA; D008836:Micrococcal Nuclease