Suppression of immunoglobulin production of lymphocytes by intravenous immunoglobulin.
The proliferative responses and the immunoglobulin production of peripheral blood mononuclear cells to pokeweed mitogen were dose-dependently suppressed by sulfonated intravenous immunoglobulin (IVIG), polyethylene glycol-treated IVIG, pH 4-treated IVIG, or human gamma-globulin, but they were not or only slightly suppressed by human serum albumin or pepsin-treated IVIG. Moreover, the suppression of immunoglobulin production by sulfonated IVIG, polyethylene glycol-treated IVIG, or pH 4-treated IVIG was seen in the cases in which B cells preincubated with IVIGs were cocultured with T cells and monocytes preincubated with or without IVIGs and in the cases in which monocytes preincubated with IVIGs were cocultured with T cells and B cells preincubated with or without IVIGs. However, in the cases in which only T cells were preincubated with IVIGs, immunoglobulin production was not suppressed. The suppression of the monocyte function by IVIGs tended to be less than the suppression of the B-cell function by IVIGs. Moreover, the suppression by IVIGs was blocked by anti-human IgG Fc. Our results suggest that IVIGs suppress the immunoglobulin production of lymphocytes through suppression of the B-cell function and the antigen presenting-cell function by attachment of IVIGs to Fc receptors of B-cell membranes and antigen presenting-cell membranes.
Kondo
N
N
Department of Pediatrics, Gifu University School of Medicine, Japan.
Ozawa
T
T
Mushiake
K
K
Motoyoshi
F
F
Kameyama
T
T
Kasahara
K
K
Kaneko
H
H
Yamashina
M
M
Kato
Y
Y
Orii
T
T
D000938:Antigen-Presenting Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000008:administration & dosage / Q000096:biosynthesis*; D007165:Immunosuppression Therapy; D066298:In Vitro Techniques; D007275:Injections, Intravenous; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D009000:Monocytes / Q000276:immunology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D011961:Receptors, Fc; D013601:T-Lymphocytes / Q000276:immunology
D007136:Immunoglobulins; D011043:Pokeweed Mitogens; D011961:Receptors, Fc