A rapid method for the preparation of yeast for immunoelectron microscopy using Lowicryl HM-20.
We describe a fixation and embedding procedure for the yeast Saccharomyces cerevisiae using Lowicryl HM-20 which is rapid, gives excellent fixation, and avoids the low temperature handling normally associated with embedding in this resin. This procedure yields superior structural preservation when compared to the commonly used rapid embedding procedure which employs Lowicryl K4M. We demonstrate that sections prepared using our rapid procedure are suitable for use in immunogold labelling experiments.
Anderson
W H
WH
Hormel Institute, University of Minnesota, Austin 55912.
Thompson
E W
EW
Zwizinski
C W
CW
D000180:Acrylic Resins; D005404:Fixatives; D016253:Microscopy, Immunoelectron / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure*; D013048:Specimen Handling
D000180:Acrylic Resins; D005404:Fixatives; C037883:Lowicryl 4KM resin; C070811:Lowicryl HM-20