Contribution of the VH11 gene family to mitogen-responsive B cell repertoire in C57BL/6 mice.
The contribution of VH11 gene family to the development of the primary B cell repertoire has been studied by analyzing 1.8 x 10(4) mitogen induced B lymphocyte colonies. The data demonstrate that VH11 family is predominantly expressed among neonatal splenic as well as adult peritoneal B cell colonies, both rich in Ly-1+ B cells. VH11 gene family expression among B splenocytes decreases during ontogeny and VH11 family pairs stochastically with different V kappa families among mitogen-activated neonatal B cell colonies, which are representative of an antigen unselected B cell repertoire. Thus, an increased VH11 expression among peritoneal and neonatal B cells points towards its biased expression among Ly-1+ B lymphocytes. The restricted V gene rearrangements and VH11-V kappa 9 pairing observed among anti-bromelain-treated mouse red blood cells autoantibodies are likely to be an outcome of both intrinsic gene recombination processes per se as well as selection by an autoantigen and/or local selective environmental factors.
Kaushik
A
A
Department of Microbiology, Mount Sinai School of Medicine, New York.
Reininger
L
L
Kelsoe
G
G
Jaton
J C
JC
Bona
C
C
D000367:Age Factors; D000818:Animals; D000916:Antibody Diversity; D001402:B-Lymphocytes / Q000502:physiology*; D015870:Gene Expression; D005803:Genes, Immunoglobulin; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics* / Q000276:immunology; D008934:Mitogens / Q000494:pharmacology; D009693:Nucleic Acid Hybridization
D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D008934:Mitogens