Survival period of tumor-bearing mice is prolonged after the interferon-gamma-producing gene transfer.
A highly tumorigenic keratinocyte-derived carcinoma cell line, designated as Pam-T, was established from a Pam212 line. The intradermal injection of more than 10(5) of these cells into syngeneic BALB/c mice induced substantial tumors. The tumors progressively enlarged and then invaded the peritoneal cavity leading to the death of the host mice. To comprehensively investigate the effects of interferon-gamma on tumorigenicity, we manufactured interferon-gamma-producing PamT cells by interferon-gamma gene transfer and examined the characteristics of the tumors induced by these cells in syngeneic mice. Interferon-gamma producing cells exhibited an apparently similar in vitro cell growth pattern and in vivo tumor formation to control cells, but the mean survival of the mice with the interferon-gamma-producing cells was significantly longer compared with control mice.
Maruguchi
Y
Y
Department of Dermatology, Faculty of Medicine, Kyoto University, Japan.
Toda
K I
KI
Fujii
K
K
Imamura
S
S
Watanabe
Y
Y
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy*; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D005260:Female; D005822:Genetic Vectors; D007371:Interferon-gamma / Q000096:biosynthesis / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009361:Neoplasm Invasiveness / Q000503:physiopathology; D011994:Recombinant Proteins; D012190:Retroviridae; D012878:Skin Neoplasms / Q000188:drug therapy*; D016019:Survival Analysis; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects*
D011994:Recombinant Proteins; D007371:Interferon-gamma