[The anomalous isoelectric properties of influenza virus matrix protein M1].
The isoelectric point (pI) values of matrix protein M1 of influenza A, B, and C viruses, calculated theoretically on the basis of its primary structure, were found to be about 10.0. However, experimental pI determination by electrofocusing in ampholyte-containing polyacrylamide gel showed it to be 5.0 for M1 protein isolated from A/WSN/33 (H1N1) and A/Aichi/2/68 (H3N2) viruses by mild deproteinization with nonionic detergents. This marked discrepancy between experimental and theoretical pI values indicated that influenza virus matrix protein M1 possessed an unusual tertiary structure and/or intensive posttranslational addition of charged residues.
Zhirnov
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D006863:Hydrogen-Ion Concentration; D009980:Influenza A virus / Q000032:analysis*; D007525:Isoelectric Focusing / Q000379:methods; D011487:Protein Conformation; D014763:Viral Matrix Proteins / Q000032:analysis* / Q000302:isolation & purification; D014771:Virion / Q000032:analysis
D014763:Viral Matrix Proteins
Anomal'nye izoélektricheskie svoĭstva matriksnogo belka M1 virusov grippa.