Monoclonal antibodies EBU-141 (CDw75) and EBU-65 allow reliable distinction between mature and pre-B-cell tumors in suspension and on tissue sections.
Two new monoclonal antibodies, EBU-65 and EBU-141, were raised by immunization with plasma cell line U-266. Both antibodies strongly react with B lymphocytes in immunofluorescent staining as well as on paraffin-embedded sections. More than 200 leukemias and lymphomas were tested, and for both antibodies reactivity was found only with "mature" B-cell tumors but not with precursor B-cell leukemias. None of the non-B-lineage hematolymphatic tumors tested was stained by EBU-141 or EBU-65. A subpopulation of T lymphocytes particularly present in nonmalignant pleural effusions was detected by EBU-65 additionally. Although EBU-141 was clustered as CDw75 and EBU-65 as "unique," a close relationship of the staining pattern was found and both antibodies react with a sialyltransferase. In particular, CDw75 antibody EBU-141 was demonstrated to be very useful for immunophenotyping of B-cell neoplasias, while EBU-65 reacted with most multiple myelomas and a subgroup of "activated"-appearing T cells.
Gramatzki
M
M
Department of Medicine III, University of Erlangen-Nürnberg, FRG.
Burger
R
R
Kraus
J
J
Lauer
U
U
Rohwer
P
P
Eger
G
G
Kalden
J R
JR
Henschke
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002143:Callitrichinae; D002460:Cell Line; D005260:Female; D005455:Fluorescent Antibody Technique; D000882:Haplorhini; D006801:Humans; D006922:Hylobates; D016130:Immunophenotyping; D007938:Leukemia / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010950:Plasma Cells / Q000276:immunology; D012453:Saimiri
D000911:Antibodies, Monoclonal; D000941:Antigens