Histochemistry of tartrate-resistant acid phosphatase and carbonic anhydrase isoenzyme II in osteoclast-like giant cells in bone tumours.
Using routinely processed, paraffin-embedded tissue specimens, osteoclast-like giant cells in giant cell tumour of bone (GCT), chondroblastoma, osteoblastoma and osteoblastic osteosarcoma were examined histochemically for osteoclast-specific enzymes tartrate-resistant acid phosphatase (TRAP) and carbonic anhydrase isoenzyme II (CA-II). Osteoclast-like giant cells and some mononuclear cells possessed TRAP activity. These were further classified with respect to CA-II immunoreactivity, i.e. cells with CA-II were seen in GCT and chondroblastoma, while those in osteoblastoma and osteoblastic osteosarcoma were negative for CA-II. All the cellular components in malignant fibrous histiocytoma and various extraosseous inflammatory lesions including malignant giant cells and macrophage polykaryons were negative for both TRAP and CA-II. These results indicate that osteoclast-like giant cells in GCT, chondroblastoma, osteoblastoma and osteoblastic osteosarcoma are all osteoclasts and generated by fusion of mononuclear cells with the same histochemical characteristics as osteoclast-like giant cells. The difference in CA-II immunoreactivity suggests the functional or maturational difference between osteoclast-like giant cells in GCT and chondroblastoma and those in osteoblastoma and osteosarcoma.
Toyosawa
S
S
Department of Oral Pathology, Osaka University Faculty of Dentistry, Japan.
Ogawa
Y
Y
Chang
C K
CK
Hong
S S
SS
Yagi
T
T
Kuwahara
H
H
Wakasa
K
K
Sakurai
M
M
D000135:Acid Phosphatase / Q000378:metabolism*; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology; D002256:Carbonic Anhydrases / Q000378:metabolism*; D004351:Drug Resistance; D015726:Giant Cells / Q000378:metabolism* / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D010010:Osteoclasts / Q000378:metabolism* / Q000473:pathology; D013644:Tartrates / Q000494:pharmacology*
D013644:Tartrates; D000135:Acid Phosphatase; D002256:Carbonic Anhydrases; C029768:tartaric acid