title: Evidence for clonal spread of a single strain of beta-lactamase-producing Enterococcus (Streptococcus) faecalis to six hospitals in five states.
abstract: Beta-lactamase-producing (Bla+) enterococci have been reported in three state and two countries. Pulsed-field gel electrophoresis was used to compare 14 Bla+ Enterococcus (Streptococcus) faecalis isolated from hospitalized patients in seven states and three continents. The restriction endonuclease digestion patterns of isolates from Connecticut, Massachusetts, Lebanon, and Argentina were all markedly different, indicating that these were different strains. However, isolates from Delaware, Texas, Pennsylvania (Philadelphia and Pittsburgh), Florida, and Virginia were similar, indicating that these isolates were derivatives of a single strain. This conclusion was supported by hybridization using individual fragments as probes. Spread of Bla+ enterococci within the hospital setting was also demonstrated. These findings illustrate the value of pulsed-field gel electrophoresis for epidemiologic analyses and support the importance of identifying and containing organisms with new resistance properties in an effort to decrease their transmission to and from, as well as within, hospitals.
authors.affiliation: Center for Infectious Diseases, University of Texas Medical School, Houston 77030.
authors.forename: B E
authors.initials: BE
authors.lastname: Murray
authors.forename: K V
authors.initials: KV
authors.lastname: Singh
authors.forename: S M
authors.initials: SM
authors.lastname: Markowitz
authors.forename: H A
authors.initials: HA
authors.lastname: Lopardo
authors.forename: J E
authors.initials: JE
authors.lastname: Patterson
authors.forename: M J
authors.initials: MJ
authors.lastname: Zervos
authors.forename: E
authors.initials: E
authors.lastname: Rubeglio
authors.forename: G M
authors.initials: GM
authors.lastname: Eliopoulos
authors.forename: L B
authors.initials: LB
authors.lastname: Rice
authors.forename: F W
authors.initials: FW
authors.lastname: Goldstein
mesh_terms: D001118:Argentina; D001900:Boston; D003237:Connecticut; D004269:DNA, Bacterial / Q000032:analysis*; D003691:Delaware; D004587:Electrophoresis, Agar Gel; D013293:Enterococcus faecalis / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D005431:Florida; D006801:Humans; D007861:Lebanon; D009693:Nucleic Acid Hybridization; D010414:Pennsylvania; D015143:Philadelphia; D010957:Plasmids; D015183:Restriction Mapping; D013290:Streptococcal Infections / Q000382:microbiology* / Q000635:transmission; D013781:Texas; D014768:Virginia; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases