Crystallization and preliminary x-ray diffraction studies of recombinant rabbit interferon-gamma.
Two different crystal forms of recombinant rabbit IFN-gamma were obtained under different crystallization conditions. The first, a tetragonal form with space group P43212 or P41212, was obtained through vapor phase equilibration using the sitting drop rods technique with ammonium citrate as the major precipitating agent. The unit cell dimensions of this crystal form are a = b = 82.1 A and C = 116.3 A. These crystals diffract to 2.8 A resolution and contain a dimer in the asymmetric unit. A second crystal form was obtained by the batch method at pH 8.0 using sodium chloride as the precipitating agent. The crystals are hexagonal, space group P6122 or P6522, and with unit cell dimensions of a = b = 58.0 A and c = 169 A. This form contains monomer in the asymmetric unit and diffracts to greater than 2.7 A resolution. Both forms appear to be eminently suitable for further analyses and crystal structure solution.
Samudzi
C T
CT
NCI-Frederick Cancer Research and Development Center, ABL-Basic Research Program, MD 21702.
Gribskov
C L
CL
Burton
L E
LE
Rubin
J R
JR
D000818:Animals; D003460:Crystallization; D007371:Interferon-gamma / Q000737:chemistry*; D011487:Protein Conformation; D011817:Rabbits; D011994:Recombinant Proteins; D014961:X-Ray Diffraction
D011994:Recombinant Proteins; D007371:Interferon-gamma