[The formation of infectious scrapie-like structures in the persistence of the agent of amyotrophic leukospongiosis in a brain cell culture].
Electron microscopic analysis of specimens from guinea-pig brain cell cultures infected with amyotrophic leucospongiosis agent (belonging to "unconventional" viruses) revealed accumulation in the culture fluid of abnormal filamentous structures similar to scrapie-associated fibrils (SAF) differing in morphology. Most of these SAF-like structures 10-15 nm in diameter contained helically wound protofilaments with a repeat at certain intervals (50-150 nm). When these structures were inoculated into guinea-pig brain astrocyte cultures they produced dystrophic-destructive changes in some (25%) astrocytes, and their intracerebral inoculation to guinea pigs produced an experimental disease. The abnormal SAF-like structures were reisolated from the brains of the inoculated animals which indicated the relationship between these structures and infectivity.
Poleshchuk
N N
NN
Il'kevich
Iu G
IuG
Kapitulets
S P
SP
Kvacheva
Z B
ZB
Luchko
V P
VP
Kolomiets
N D
ND
Votiakov
V I
VI
D000818:Animals; D001253:Astrocytes / Q000382:microbiology; D001921:Brain / Q000166:cytology / Q000382:microbiology*; D001927:Brain Diseases / Q000382:microbiology*; D006168:Guinea Pigs; D008854:Microscopy, Electron; D009046:Motor Neurons; D009468:Neuromuscular Diseases / Q000382:microbiology*; D011328:Prions / Q000302:isolation & purification* / Q000472:pathogenicity / Q000648:ultrastructure; D012897:Slow Virus Diseases / Q000382:microbiology*; D014776:Virus Cultivation
D011328:Prions
Formirovanie infektsionnykh skrepipodobnykh struktur pri persistentsii agenta amiotroficheskogo leĭkospongioza v kul'ture kletok mozga.