A general ampC active site oligonucleotide probe for gram-negative rods.
We have developed a 33 mer probe that hybridizes to the serine active site of the chromosomal ampC beta-lactamase gene of Pseudomonas aeruginosa and the Enterobacteriaceae. We tested this probe against a variety of Enterobacteriaceae, and a series of 23 P. aeruginosa by dot-blots and selected Southern blots. This probe is an alternative or supplement to enzyme studies for characterizing the class of a Gram-negative rod's beta-lactamase and is a useful tool for studies of Pseudomonas beta-lactamase regulation.
Willard
K E
KE
Laboratory Service, Veterans Administration Medical Center, Minneapolis, MN.
Moody
J A
JA
Peterson
L R
LR
D001483:Base Sequence; D001665:Binding Sites; D004755:Enterobacteriaceae / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D012694:Serine; D001618:beta-Lactamases / Q000235:genetics*
D015345:Oligonucleotide Probes; D012694:Serine; D001618:beta-Lactamases