Canine carrageenin-induced acute paw inflammation model and its response to nonsteroidal antiinflammatory drugs.
A quantitative method for testing antiinflammatory agents in beagles has been developed, based on measurement of paw inflammation induced by a local injection of carrageenin. Carrageenin [0.5 mL of 2% (wt/vol) in saline] was injected into the plantar region of the hindpaws of pentobarbital-anesthetized beagles. Paw pressure changes registered from a water-filled balloon held on the top of the paw by a light adhesive tape wrapping were monitored for 240 min. In control dogs given 0.5% (wt/vol) methylcellulose (10 mL/kg orally) just before carrageenin, paw pressure increased significantly (p less than 0.05) over eightfold, from 2.9 +/- 0.8 mm Hg (mean +/- SEM, n = 29 paws) at 75 min to 26.0 +/- 3.5 mmHg at 240 min. The increase in paw pressure was significantly inhibited by the cyclooxygenase inhibitors, ibuprofen, indomethacin, and orpanoxin, and partially inhibited by the lipoxygenase inhibitor, phenidone, administered orally before carrageenin injection. Thus this model, with further characterization, could provide a convenient, quantitative way of assessing the efficacy of nonsteroidal antiinflammatory agents in dogs.
Brooks
R R
RR
Product Development, Norwich Eaton Pharmaceuticals, Inc., A Procter & Gamble Company, Norwich, New York 13815-0191.
Carpenter
J F
JF
Jones
S M
SM
Ziegler
T C
TC
Pong
S F
SF
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000493:pharmacokinetics / Q000494:pharmacology*; D002351:Carrageenan; D016861:Cyclooxygenase Inhibitors; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D005528:Foot / Q000473:pathology; D007249:Inflammation / Q000139:chemically induced* / Q000503:physiopathology; D016859:Lipoxygenase Inhibitors; D008297:Male
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016861:Cyclooxygenase Inhibitors; D016859:Lipoxygenase Inhibitors; D002351:Carrageenan