A DNA primer/probe system for the rapid and sensitive detection of Mycobacterium tuberculosis-complex pathogens.
A 1.5 kb EcoRI-BamHI restriction fragment from Mycobacterium tuberculosis was found to hybridize specifically with genomic DNA from M. tuberculosis-complex organisms. Primers were designed from the terminal sequences of this fragment and used to amplify uniquely M. tuberculosis-group DNA in a polymerase chain reaction. It is suggested that a combination of these primers and probe will prove a useful tool for the early diagnosis of tuberculous infections.
Ralphs
N T
NT
Department of Microbiology, University of Leicester, UK.
Garrett
S
S
Morse
R
R
Cookson
J B
JB
Andrew
P W
PW
Boulnois
G J
GJ
D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics*; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction
D015342:DNA Probes; D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI