Screening of Candida sp. for N-dealkylation of codeine.
A number of Candida species were selected in order to test their ability to N- or O-demethylate codeine. Complex media and chemically defined media used in our experiments. Transformation products were analysed by reversed phase HPLC and TLC methods. N- and O-demethylation of codeine was performed by C. tropicalis NCYC 997, NCYC 405, NCYC 470 and C. albicans LSHTM 3153. Transformation by C. tropicalis NCYC 997 had better results. N- and O-demethylation occurred in the chemically defined medium. Change in the colour of the transformation mixture, in cultures containing more than 5 mmol.l-1 codeine, was attributed to codeine N-oxide, but its presence was not confirmed by HPLC. N- and O-demethylation of codeine by Candida sp. was compared to that occurred in mammals.
Stavrianakis
I G
IG
School of Pharmacy and Pharmacology, University of Bath, U.K.
Soper
C J
CJ
D002175:Candida / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003061:Codeine / Q000378:metabolism*; D003470:Culture Media; D003640:Dealkylation
D003470:Culture Media; D003061:Codeine