Parameters affecting susceptibility of PCR contamination to UV inactivation.
Contamination of reagents used for PCR is a serious problem. We have recently reported the remarkable effectiveness of UV light in successful decontamination of PCR reagents when the reagents were contaminated with a 6-kb plasmid, followed by amplification of 750-bp segment from the insert. However, further investigation reveals that segment size, sequence and hydration can have a dramatic effect on the efficiency of UV inactivation. Despite some limitations, UV remains a highly effective means of decontamination.
Sarkar
G
G
Department of Biochemistry and Molecular Biology, Mayo Clinic/Foundation, Rochester, MN 55905.
Sommer
S S
SS
D004247:DNA / Q000528:radiation effects; D004340:Drug Contamination / Q000517:prevention & control*; D005164:Factor IX / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D013997:Time Factors; D014466:Ultraviolet Rays
D005164:Factor IX; D004247:DNA