Inhibitory effect of 2-O-octadecylascorbic acid in agglutination assay with concanavalin A; short-term examination of rat urinary bladder carcinogenesis.
A derivative of ascorbic acid, 2-O-octadecylascorbic acid (CV-3611), is a strong scavenger of active oxygen species. We examined the effect of CV-3611 on a short-term test of bladder carcinogenesis, using concanavalin A (Con A)-dependent agglutination of isolated bladder epithelial cells. Rats were given 0.01% N-butyl-N-(4-hydroxybutyl)nitrosamine (BHBN) for 1 week, and then 5% sodium saccharin or 2% DL-tryptophan or 0.01% BHBN alone or with 0.002, 0.006 or 0.02% CV-3611 for 3 weeks. Treatment with CV-3611 reduced the effects of the bladder tumor promoters sodium saccharin and DL-tryptophan by 48-86 and 65-87%, respectively. CV-3611 also reduced the number of aggregates of bladder epithelial cells from rats treated with BHBN for 4 weeks. These results suggest that CV-3611 has a suppressive effect on rat bladder carcinogenesis.
Suzuki
M
M
National Cancer Center Research Institute, Tokyo.
Wakabayashi
K
K
Sone
H
H
Kushida
H
H
Sugiyama
K
K
Kakizoe
T
T
Nagao
M
M
Sugimura
T
T
D000372:Agglutination Tests; D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D002085:Butylhydroxybutylnitrosamine; D002449:Cell Aggregation / Q000187:drug effects*; D003208:Concanavalin A; D016166:Free Radical Scavengers; D008297:Male; D051381:Rats; D012439:Saccharin; D014364:Tryptophan; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced* / Q000188:drug therapy
D016166:Free Radical Scavengers; D003208:Concanavalin A; D002085:Butylhydroxybutylnitrosamine; D014364:Tryptophan; C061185:2-O-octadecylascorbic acid; D012439:Saccharin; D001205:Ascorbic Acid