title: A disialoganglioside of the globo-series from chicken skeletal muscle.
abstract: We have isolated a disialoganglioside of the globo-series from chicken pectoral muscle. The compound was obtained by extraction followed by ion-exchange and silicic acid column chromatography and judged to be pure by thin-layer chromatography in three solvent systems. The structure of the ganglioside was determined by carbohydrate and ceramide composition analysis, sequential exoglycosidase digestion, methylation analysis, and 500-MHz 1H-NMR spectroscopy to be: (formula; see text) Analysis of the ceramide moiety indicated d18:1 sphingosine as the long-chain base, and C16:0, C18:0, C18:1, and C20:0 as the prevalent fatty acids. This glycolipid is only the second ganglioside of the globo-series, and the first disialo member of the series, found in chicken muscle.
authors.affiliation: Department of Neurology, Medical University of South Carolina, Charleston.
authors.forename: S
authors.initials: S
authors.lastname: Dasgupta
authors.forename: J L
authors.initials: JL
authors.lastname: Chien
authors.forename: E L
authors.initials: EL
authors.lastname: Hogan
authors.forename: H
authors.initials: H
authors.lastname: van Halbeek
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002645:Chickens; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000737:chemistry / Q000302:isolation & purification*; D006026:Glycoside Hydrolases / Q000378:metabolism; D008055:Lipids / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009132:Muscles / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D005732:Gangliosides; D008055:Lipids; D006026:Glycoside Hydrolases