[Modifications of sialylation in BHK 21/C13 cells after in vitro transfection by c-Ha-ras human oncogene].
A Hamster kidney fibroblast cell line (BHK 21/C13) has been transfected by c-Ha-ras human oncogene. The expression of the oncogene significantly modified the global sialytransferase activity of the cell extract. This activity is enhanced on an average 2.5 fold whatever the acceptor. In addition, the proportion of cell-surface associated N-glycolylneuraminic acid is enhanced in transfectants, the ratio N-acetylneuraminic acid/N-glycolylneuraminic acid decreases from 7.10 to 2.80. These results suggest that a tight relationship exists, in c-Ha-ras transfected BHK cells, between the expression of the oncogene and the neuraminic acid metabolism as well as a gene regulation of glycoconjugate sialylation.
Cazlaris
H
H
Université des Sciences et Techniques de Lille Flandres-Artois, Unité mixte de Recherches du C.N.R.S. n.111, Villeneuve-d'Ascq.
Le Marer
N
N
Laudet
V
V
Lagrou
C
C
Zhu
Q
Q
Delannoy
P
P
Montreuil
J
J
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000166:cytology*; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics*; D012794:Sialic Acids / Q000378:metabolism*; D014162:Transfection
D012794:Sialic Acids; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)
Modifications de la sialylation des cellules BHK 21/C13 après transfection in vitro par l'oncogène humain c-Ha-ras.