[Enzyme immunoassay for secretory immunoglobulin A on skin surface and in sweat].
Recently secretory IgA (S-IgA) was found to be secreted from the eccrine gland. By using a sandwich enzyme immunoassay, we measured the concentration of S-IgA 1) in sweat (sweat S-IgA) and 2) in the extract buffer obtained by pipetting++ on the skin (skin surface S-IgA). Skin surface hydration and skin surface lipid were measured at the sites where the samples of skin surface S-IgA were collected. (These measurements were taken immediately after the buffer was pipetted). The quantity of both sweat S-IgA and skin surface S-IgA differed according to the sites where they were collected. Sites in order of decreasing sweat S-IgA level: face, chest, forearm. Sites in order of decreasing skin surface S-IgA level: face, chest, palm, forearm, sole. (Skin surface S-IgA levels on palm and forearm were approximately equal). Although the amount of skin surface S-IgA was not related to the skin surface hydration, there was a significant correlation between skin surface S-IgA and skin surface lipid.
Kawai
K
K
Kyoto Prefectural University of Medicine.
Kawai
K
K
Konishi
K
K
Kishimoto
S
S
Kanemitsu
K
K
Kamei
N
N
Obayashi
H
H
Okada
T
T
Konishi
H
H
D000293:Adolescent; D000328:Adult; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D008055:Lipids / Q000032:analysis; D008297:Male; D012867:Skin / Q000737:chemistry*; D013048:Specimen Handling; D013542:Sweat / Q000737:chemistry*
D007071:Immunoglobulin A, Secretory; D008055:Lipids