Amino acid sequence of the FV region of a human monoclonal IgM (NOV) with specificity for the capsular polysaccharide of the group B meningococcus and of Escherichia coli K1, which cross-reacts with polynucleotides and with denatured DNA.
The complete amino acid sequences of the VH and VL regions of a biologically significant Ig, IgMNOV, were determined. IgMNOV is reactive with the capsular polysaccharide of the group B meningococcus and of Escherichia coli K1. As reported earlier, it cross-reacts completely with polynucleotides poly(A) and poly(I) and to a lesser extent with denatured DNA and protects newborn rats against infection with E. coli K1, and is equal in potency to the standard horse anti-group B meningococcal serum. The reduced and alkylated chains were sequenced directly, identifying the L chain as lambda-subgroup II and the mu-H chain as subgroup III. The complete sequence of the VL region was determined by sequencing peptides generated by cleavage with Staphylococcus aureus protease, chymotrypsin, and trypsin. The H chain was cleaved with cyanogen bromide followed by enzymatic cleavages to obtain a large part of the VH region sequence. The structure was completed by sequencing tryptic peptides of the Fab fragment and by mass-spectrometric analysis.
Gawinowicz
M A
MA
Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Merlini
G
G
Birken
S
S
Osserman
E F
EF
Kabat
E A
EA
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D004247:DNA / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007075:Immunoglobulin M / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000276:immunology*; D011061:Poly A / Q000276:immunology; D011069:Poly I / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000276:immunology*
D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D011135:Polysaccharides, Bacterial; D011061:Poly A; D011069:Poly I; D004247:DNA