IFN-gamma retards the turnover of H-2Dd antigens by splenic lymphocytes.
The effect of IFN-gamma on the rate of shedding and biosynthesis of H-2Dd was determined by culture of cell surface-radioiodinated BALB/c spleen cells with rIFN-gamma or spleen cells metabolically labeled with 35S-methionine in the presence of IFN-gamma. Radioiodinated or 35S-labeled H-2Dd was quantitated by immunoprecipitation of H-2Dd from detergent lysates of radiolabeled cells taken at different culture intervals. The loss of 125I-labeled H-2Dd was retarded 75 to 90% by IFN-gamma whereas the biosynthetic rate was unaffected during the first 10-h culture. The net result was a ninefold increase in newly synthesized cell-associated H-2Dd. The results were consistent with determination of the kinetics of increased expression of H-2Dd determined by immunofluorescence and suggest that an early effect of IFN-gamma on the expression of class I Ag is a retardation of catabolism leading to an increase of newly synthesized class I Ag.
Guha
A
A
Department of Pathology (Division of Experimental Pathology), University of Connecticut Health Center, Farmington 06032.
Cone
R E
RE
D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis / Q000378:metabolism; D005260:Female; D006183:H-2 Antigens / Q000096:biosynthesis / Q000378:metabolism*; D064532:Histocompatibility Antigen H-2D; D007371:Interferon-gamma / Q000502:physiology*; D007457:Iodine Radioisotopes; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology; D013997:Time Factors
D000954:Antigens, Surface; D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D; D007457:Iodine Radioisotopes; D008562:Membrane Glycoproteins; D007371:Interferon-gamma