Leukemia inhibitory factor sustains the survival of mouse primordial germ cells cultured on TM4 feeder layers.
Various growth factors and cytokines were tested for their effects on survival and proliferation of mouse primordial germ cells (PGCs) cultured on TM4 cell feeder layers. Leukemia inhibitory factor was able to sustain the survival of PGCs from 10.5 dpc embryos for at least 3 days and to slow down degeneration of PGCs from 11.5 dpc embryos cultured on TM4 feeder layers.
De Felici
M
M
Department of Public Health, II University of Rome, Italy.
Dolci
S
S
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005854:Germ Cells / Q000166:cytology* / Q000187:drug effects; D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology*; D008297:Male; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology; D012708:Sertoli Cells
D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D011994:Recombinant Proteins