Distribution of the alpha 1-alpha 6 integrin subunits in human developing and term placenta.
The distribution of the alpha 1-alpha 6 as well as alpha v, beta 1, beta 3 and beta 4 integrin subunits in human first and second trimester and term placentas was studied by indirect immunofluorescence microscopy using a panel of monoclonal antibodies (mAbs). In first and second trimester villi, the alpha 1 and beta 1 integrin subunits were detected in the stromal cells, that were mostly also immunoreactive for desmin. Desmin-positive stromal cells were also found in villi of term placentas, but the stroma was negative for anti-alpha 1 and -beta 1. In the villous trophoblast, anti-alpha 6 and -beta 4 revealed a distinct basal immunoreactivity during all stages of development, whereas immunoreactivity for the alpha 3 and beta 1 subunits emerged during the second and third trimesters. Throughout placental development, endothelia of villous capillaries reacted prominently with anti-alpha 1 and -beta 1. Intermediate trophoblastic cells displayed a somewhat heterogenous immunoreactivity for the beta 1, alpha 1, alpha 3 and alpha 5 integrin subunits, and differed from villous trophoblast also in their lack of expression of the alpha 6 and beta 4 subunits. While nondecidualized endometrial cells displayed weak reactivity for the alpha 1 and beta 1 integrin subunits, the individual decidual cells presented both a basement membrane and a cell surface-confined immunoreactivity for anti-alpha 1, -alpha 3, and -beta 1. The results suggest a role for integrins in placental development, and show that expression of integrins is modulated during the differentiation of trophoblast, villous stroma, and decidual cells. Furthermore, the basal localization of alpha 6 beta 4 and alpha 3 beta 1 integrins suggests that they are employed as basement membrane receptors in the villous trophoblast, and the emergence of the alpha 3 beta 1 complex may reflect that the cytotrophoblast and syncytiotrophoblast recognize the basement membrane differently.
Korhonen
M
M
Department of Anatomy, University of Helsinki, Finland.
Ylänne
J
J
Laitinen
L
L
Cooper
H M
HM
Quaranta
V
V
Virtanen
I
I
D002196:Capillaries / Q000378:metabolism; D003656:Decidua / Q000378:metabolism; D036861:Delivery, Obstetric; D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008871:Microvilli / Q000378:metabolism; D010920:Placenta / Q000098:blood supply / Q000378:metabolism* / Q000502:physiology; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011262:Pregnancy Trimester, Second; D014018:Tissue Distribution; D014327:Trophoblasts / Q000378:metabolism
D016023:Integrins