[Behavioral and histochemical observation in rotational rat with adrenal medulla transplantation--comparison of two different graft sites (striatum v.s. lateral ventricle)].
Effect of adrenal medullary allograft to two different sites of the brain (striatum and lateral ventricle) was compared on rotation rat. Rotation rats were prepared by chemical ablation of the right nigro-striatal system by 6-hydroxy-dopamine (6-OHDA). In this experiment, adrenal medulla was transplanted to the rotation rat brain and effects of the transplantation was evaluated by behavioral change of the rats, i.e., reduction in rotation behavior, and by histochemical examination of the grafts sites, i.e., presence and morphology of dopaminergic cells. The animals were divided in to 2 groups. The first group received the graft to the striatum (striatum group) and the second group to the lateral ventricle close to the striatum (ventricle group). Six weeks after grafting, the rotation behavior in striatum group and ventricle group was reduced by 43% (p less than 0.01) and 35% (p less than 0.01) respectively as compared to that in the pretransplantation state. Twelve weeks after grafting, the comparable figures were 30% (p less than 0.05) and 17% (n.s.) respectively. Histochemical examination of graft site was similar in both groups: Six weeks after grafting, many transplanted cells transformed into nerve cells which were considered to be capable of producing dopamine and, twelve weeks after grafting, honeycomb shaped fluorescence positive area without apparent viable cells. From above results, it was concluded that the striatum was somewhat superior to the lateral ventricle as the graft site. However, the effect of the transplantation was short lasting, being most manifest at six weeks after transplantation, and started to wear off as the grafted cells perished.
Torigoe
R
R
Department of Neurosurgery, St. Mary's Hospital, Fukuoka, Japan.
Hayashi
T
T
Anegawa
S
S
Yoshida
M
M
Ohtsuru
K
K
D000313:Adrenal Medulla / Q000378:metabolism / Q000637:transplantation*; D000818:Animals; D001522:Behavior, Animal; D002552:Cerebral Ventricles / Q000601:surgery; D003342:Corpus Striatum / Q000601:surgery; D004298:Dopamine / Q000378:metabolism; D006651:Histocytochemistry; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012399:Rotation; D016042:Transplantation, Heterotopic
D004298:Dopamine