title: Correlation between the "low"-salt-induced increase in the F730/F685 fluorescence emission ratio at 77 K in isolated chloroplasts, and the organization of chlorophyll in photosystem I pigment-protein complexes of thylakoids.
abstract: Isolated pea or spinach chloroplasts suspended in "high"-salt phosphate buffer exhibit a low F730/F685 fluorescence emission ratio at 77 K; in contrast, removal of cations by incubation in "low"-salt Tricine buffer induces a drastic increase in the F730/F685 ratio. Parallel to the F730/F685 ratio increase, a gradual organization of chlorophyll (Chl) in the pigment-protein complexes of the Photosystem I, chlorophyll-protein complex Ia, and light-harvesting complex I (LHC-I), is observed. The kinetics of the two processes are closely correlated, all changes being completed within 5-10 min from Tricine addition. On the other hand, the inability of low-salt Tricine to induce any changes in the F730/F685 ratio in bean plastids, isolated and suspended in high-salt phosphate buffer, correlates with the lack of extensive changes in the organization of the Photosystem I complexes, and more specifically of LHC-I. The latter is attributed to the higher stability of complexes in bean, arising from stronger association of thylakoids in grana stacks in this species; this is probably due to higher levels of residual divalent cations present in the isolated thylakoids of bean compared to pea (or spinach). The results suggest that the F730/F685 ratio changes, observed in chloroplasts by manipulation of their ionic environment, reflect modulation of Chl organization within the pigment-protein complexes of the photosynthetic units.
authors.affiliation: Institute of Biology, NRC Demokritos, Aghia Paraskevi, Attiki, Athens, Greece.
authors.forename: J
authors.initials: J
authors.lastname: Argyroudi-Akoyunoglou
mesh_terms: D002021:Buffers; D002458:Cell Fractionation; D002501:Centrifugation, Zonal; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism*; D007887:Fabaceae / Q000378:metabolism; D005615:Freezing; D007700:Kinetics; D008027:Light; D045342:Light-Harvesting Protein Complexes; D015388:Organelles / Q000378:metabolism*; D009994:Osmolar Concentration; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D045331:Photosystem I Protein Complex; D010944:Plants / Q000378:metabolism*; D010946:Plants, Medicinal; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D002021:Buffers; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D002734:Chlorophyll