Miniaturization of three carbohydrate analyses using a microsample plate reader.
Three carbohydrate analyses (reducing value by copper-bicinchoninate, total carbohydrate by phenol-sulfuric acid, and D-glucose by glucose oxidase) have been miniaturized using a microsample plate reader. The use of the reducing-value procedure to measure the hydrolysis of starch by alpha-amylase and the use of the glucose oxidase method to measure the hydrolysis of lactose by lactase are illustrated.
Fox
J D
JD
Department of Biochemistry and Biophysics, Iowa State University, Ames 50011.
Robyt
J F
JF
D002241:Carbohydrates / Q000032:analysis*; D003300:Copper; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase / Q000032:analysis; D007202:Indicators and Reagents; D008832:Microchemistry / Q000295:instrumentation; D008904:Miniaturization; D010084:Oxidation-Reduction; D019800:Phenol; D010636:Phenols; D011804:Quinolines; D013464:Sulfuric Acids; D000516:alpha-Amylases / Q000032:analysis
D002241:Carbohydrates; D007202:Indicators and Reagents; D010636:Phenols; D011804:Quinolines; D013464:Sulfuric Acids; D019800:Phenol; D003300:Copper; C047117:bicinchoninic acid; D005949:Glucose Oxidase; D000516:alpha-Amylases; D005947:Glucose; C033158:sulfuric acid