Hepatic and renal expression of rat apolipoprotein E under control of the metallothionein promoter in transgenic mice.
We created three lines of transgenic mice with an integrated rat genomic apolipoprotein E gene fused with the mouse metallothionein I promoter. These lines transcribed rat apoE mRNA in the liver and/or in the kidney and expressed significant amounts of rat apoE in plasma. Enhancement of the plasma level by treatment with Zn ion or Bi ion was observed.
Shimano
H
H
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Yamada
N
N
Shimada
M
M
Ohsawa
N
N
Fukazawa
C
C
Yazaki
Y
Y
Takaku
F
F
Katsuki
M
M
D000818:Animals; D001057:Apolipoproteins E / Q000097:blood / Q000235:genetics*; D001483:Base Sequence; D001729:Bismuth / Q000378:metabolism; D015152:Blotting, Northern; D015139:Blotting, Southern; D005786:Gene Expression Regulation / Q000502:physiology; D015151:Immunoblotting; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008668:Metallothionein / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis; D015032:Zinc / Q000378:metabolism
D001057:Apolipoproteins E; D011994:Recombinant Proteins; D008668:Metallothionein; D015032:Zinc; D001729:Bismuth