Reduction of the in vitro hemolytic activity of soybean lecithin liposomes by treatment with a block copolymer.
The in vitro hemolytic activity of liposomes made of soybean L-alpha-lecithin towards diluted (0.0086 v/v) human erythrocytes was used to investigate the effect of surface coating on the interaction of liposomes with cells. The increase in apparent volume of the block copolymer of ethylene glycol and propylene glycol, Pluronic F-127, in the presence of liposomes supports the hypothesis of either adsorption or penetration of the copolymer at the surface of the liposomes. When the liposomes are pre-incubated with Pluronic F-127, their lytic activity towards fresh erythrocytes is significantly reduced while it remains unchanged towards erythrocytes aged in vitro. It is also found that aging the liposomes has little effect on their lytic activity while aging of the erythrocytes makes them more fragile towards the liposomes. The results are discussed in terms of steric hindrance.
Quirion
F
F
INRS-Santé, Pointe-Claire (Québ.), Canada.
St-Pierre
S
S
D004912:Erythrocytes / Q000187:drug effects; D019855:Ethylene Glycol; D005026:Ethylene Glycols; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes; D009996:Osmotic Fragility / Q000187:drug effects; D010713:Phosphatidylcholines / Q000737:chemistry / Q000494:pharmacology*; D011060:Poloxalene; D019946:Propylene Glycol; D011409:Propylene Glycols; D013025:Glycine max / Q000032:analysis
D005026:Ethylene Glycols; D008081:Liposomes; D010713:Phosphatidylcholines; D011409:Propylene Glycols; D019946:Propylene Glycol; D011060:Poloxalene; D019855:Ethylene Glycol