title: Isolation of a novel arachidonic acid metabolite 3-hydroxy-5,8,11,14-eicosatetraenoic acid (3-HETE) from the yeast Dipodascopsis uninucleata UOFs-Y128.
abstract: When arachidonic acid (AA) is added to the yeast Dipodascopsis uninucleata UOFS Y128, one of the major metabolites isolated and purified with the help of thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) is 3-hydroxy-5,8,11,14-eicosatetraenoic acid (3-HETE). The structure of this new AA metabolite was elucidated mainly by electron impact (EI) mass spectrometry (MS). Strikingly, the formation of this new metabolite was found to be inhibited by aspirin.
authors.affiliation: Department of Microbiology and Biochemistry, University of the Orange Free State, Bloemfontein, South Africa.
authors.forename: M S
authors.initials: MS
authors.lastname: van Dyk
authors.forename: J L
authors.initials: JL
authors.lastname: Kock
authors.forename: D J
authors.initials: DJ
authors.lastname: Coetzee
authors.forename: O P
authors.initials: OP
authors.lastname: Augustyn
authors.forename: S
authors.initials: S
authors.lastname: Nigam
mesh_terms: D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D001241:Aspirin / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006893:Hydroxyeicosatetraenoic Acids / Q000737:chemistry / Q000302:isolation & purification*; D013058:Mass Spectrometry; D004718:Saccharomycetales / Q000187:drug effects / Q000378:metabolism*; D013053:Spectrophotometry
chemical_list: D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; C069265:3-hydroxy-5,8,11,14-eicosatetraenoic acid; D016718:Arachidonic Acid; D001241:Aspirin