Human T-lymphocytes synthesize and secrete a protease resistant proteoglycan in a delayed, serum-dependent response to concanavalin A.
In the presence of radioactive precursors, the addition of Concanavalin A (Con A) to cultures of mononuclear cells (MC) from human blood resulted in marked accumulation in the medium of a protease-resistant proteoglycan (PG) with chondroitin sulfate (CS) chains of an average molecular weight of 28 KDa. This PG was shown to be elaborated primarily by T-cells. The addition of serum was necessary for maximal stimulation with Con A and under these conditions PG accumulation in the medium rose slowly during the first few days of culture and much more rapidly between days 6-8.
Chopra
R
R
Department of Medicine, Queen's University, Kingston, Ontario, Canada.
Griffith
E
E
Irwin
D
D
Anastassiades
T
T
D001769:Blood; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000032:analysis; D003208:Concanavalin A / Q000494:pharmacology*; D010450:Endopeptidases / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008213:Lymphocyte Activation; D008970:Molecular Weight; D011509:Proteoglycans / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*
D011509:Proteoglycans; D003208:Concanavalin A; D002809:Chondroitin Sulfates; D010450:Endopeptidases