title: An SfiI restriction map of the Bacillus subtilis 168 genome.
abstract: A restriction map of 24 SfiI (GGCCN4/NGGCC) restriction fragments has been constructed for the Bacillus subtilis genome. The combined sizes of the fragments indicate a genome size of approx. 4.2 Mb. The SfiI fragments range in size from 7-730 kb. Genetic markers have been located on 19 of the SfiI fragments, and 69 genetic markers have been assigned to the SfiI restriction map.
authors.affiliation: Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140.
authors.forename: M
authors.initials: M
authors.lastname: Amjad
authors.forename: J M
authors.initials: JM
authors.lastname: Castro
authors.forename: H
authors.initials: H
authors.lastname: Sandoval
authors.forename: J J
authors.initials: JJ
authors.lastname: Wu
authors.forename: M
authors.initials: M
authors.lastname: Yang
authors.forename: D J
authors.initials: DJ
authors.lastname: Henner
authors.forename: P J
authors.initials: PJ
authors.lastname: Piggot
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D015139:Blotting, Southern; D015252:Deoxyribonucleases, Type II Site-Specific; D004586:Electrophoresis; D005798:Genes, Bacterial; D005819:Genetic Markers; D015183:Restriction Mapping
chemical_list: D005819:Genetic Markers; C041749:endodeoxyribonuclease SfiI; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases