Celiac trunk cannulation for obtaining abomasal lymph from cattle.
Cannulation of the celiac trunk was surgically performed in 26 Holstein steers. The procedure was successful in 23 (88.5%) of the steers. Twenty-two of the steers were infected either naturally or experimentally with the abomasal nematode, Ostertagia ostertagi and/or other gastrointestinal parasites. The remaining 4 steers were not infected. Lymph obtained after surgery was used in various immunologic and biochemical assays. Daily lymph flow rate and total and differential WBC counts were determined after surgery in 4 of the infected and 3 of the noninfected steers. Steers were euthanatized for tissue specimen collection 7 days after surgery. At the time of euthanasia, lymph was still flowing from the cannula of 13 (56.5%) of the steers in which surgery was successful. This surgical procedure represents a valuable technique for studying at the local level, immunologic and physiologic responses of cattle to infection with O ostertagi.
Baker
D G
DG
Department of Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
Gershwin
L J
LJ
Snider
T G
TG
D000818:Animals; D002404:Catheterization / Q000662:veterinary*; D002417:Cattle / Q000601:surgery*; D002418:Cattle Diseases / Q000276:immunology / Q000503:physiopathology; D007958:Leukocyte Count / Q000662:veterinary; D008196:Lymph / Q000502:physiology*; D008208:Lymphatic System / Q000601:surgery*; D008297:Male; D010029:Ostertagiasis / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary; D013048:Specimen Handling / Q000662:veterinary*