Structural features of methyl-accepting taxis proteins conserved between archaebacteria and eubacteria revealed by antigenic cross-reaction.
A number of eubacterial species contain methyl-accepting taxis proteins that are antigenically and thus structurally related to the well-characterized methyl-accepting chemotaxis proteins of Escherichia coli. Recent studies of the archaebacterium Halobacterium halobium have characterized methyl-accepting taxis proteins that in some ways resemble and in other ways differ from the analogous eubacterial proteins. We used immunoblotting with antisera raised to E. coli transducers to probe shared structural features of methyl-accepting proteins from archaebacteria and eubacteria and found substantial antigenic relationships. This implies that the genes for the contemporary methyl-accepting proteins are related through an ancestral gene that existed before the divergence of arachaebacteria and eubacteria. Analysis by immunoblot of mutants of H. halobium defective in taxis revealed that some strains were deficient in covalent modification of methyl-accepting proteins although the proteins themselves were present, while other strains appeared to be missing specific methyl-accepting proteins.
Alam
M
M
Department of Biochemistry/Biophysics, Washington State University, Pullman 99164-4660.
Hazelbauer
G L
GL
D001105:Archaea / Q000276:immunology / Q000502:physiology*; D001419:Bacteria / Q000276:immunology; D018407:Bacterial Physiological Phenomena; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology / Q000648:ultrastructure; D002633:Chemotaxis; D003429:Cross Reactions; D006217:Halobacterium / Q000276:immunology / Q000502:physiology; D008565:Membrane Proteins / Q000737:chemistry / Q000276:immunology / Q000648:ultrastructure*; D000072236:Methyl-Accepting Chemotaxis Proteins; D008745:Methylation; D008970:Molecular Weight; D009154:Mutation; D015398:Signal Transduction
D001426:Bacterial Proteins; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins