[Resistant action of gangliosides on the 6-OHDA injured sympathetic nerves].
A single dose of 6-OHDA (15 mg/kg i.p.) would cause an almost complete disappearance of catecholamine fluorescence in histological preparation of submandibular glands (SMG) 24 hours after injection. Recovery of NA and DA levels went on slowly along with the course of regeneration of the lesioned sympathetic nerve terminals. The NA and the DA contents reached 50% and 28% respectively two weeks after lesion and fully recovered by the end of the fourth week. Gangliosides mixture (GM) (50 mg/kg i.p.) given 0, 4, 8, and 12 hours after 6-OHDA injection could prevent the injury action of NA and DA for at least 24 hours. If this treatment was followed by daily injection for one week, the NA contents remained normal by the end of two weeks. GM treatments beginning either 4 days before or 4 hours after 6-OHDA injection could lessen the extent of decrease of NA and DA by 6-OHDA. The results suggest that: a) GM is effective in protecting the membrane of sympathetic terminals from the injurious effect of 6-OHDA or 6-OHDA metabolites and promoting the regeneration of the lesioned terminals. b) The earlier the GM treatments are given, the better will be the effects.
Wang
J Z
JZ
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing.
Liu
C A
CA
Hua
Z W
ZW
Wu
W P
WP
D000320:Adrenergic Fibers / Q000187:drug effects*; D000818:Animals; D004298:Dopamine / Q000378:metabolism; D005260:Female; D005732:Gangliosides / Q000494:pharmacology*; D006892:Hydroxydopamines / Q000494:pharmacology*; D051379:Mice; D009416:Nerve Regeneration / Q000187:drug effects; D009638:Norepinephrine / Q000378:metabolism; D016627:Oxidopamine; D012469:Salivary Glands / Q000378:metabolism
D005732:Gangliosides; D006892:Hydroxydopamines; D016627:Oxidopamine; D004298:Dopamine; D009638:Norepinephrine