Detection of Anaplasma marginale rickettsemia prior to onset of clinical signs by using an antigen capture enzyme-linked immunosorbent assay.
An antigen capture enzyme-linked immunosorbent assay was developed by using monoclonal antibodies to conserved epitopes on the Anaplasma marginale MSP1a surface protein. The assay sensitivity was 1.1 (+/- 0.5)% parasitized erythrocytes, and all infected cattle were detected prior to development of 2.0%-parasitized erythrocytes. Positive tests preceded the onset of anemia by a mean of 2 days. The assay was specific for anaplasmosis, as demonstrated by nonreactivity with another common hemoparasitic pathogens.
Trueblood
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Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.
McGuire
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TC
Palmer
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GH
D000712:Anaplasmosis / Q000175:diagnosis*; D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000175:diagnosis / Q000662:veterinary
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial