Microencapsulation of isolated pituitary cells by polyacrylamide microlatex coagulation on agarose beads.
Microlatex beads of homogenous size were made by polymerization of a mixture of acrylamide/bisacrylamide dispersed in a microemulsion. The microlatex was aggregated by dilution of the microemulsion in acrylamide solutions. The aggregates were then coagulated by polymerization at the interfaces of agarose beads circulating in a capillary tube containing paraffin oil. Biocompatibility was tested on isolated pituitary cells microencapsulated by this procedure.
Dupuy
B
B
INSERM U.306, Université de Bordeaux II, France.
Cadic
C
C
Gin
H
H
Baquey
C
C
Dufy
B
B
Ducassou
D
D
D020106:Acrylamide; D000178:Acrylamides / Q000737:chemistry* / Q000633:toxicity; D000818:Animals; D001672:Biocompatible Materials; D002214:Capsules; D002478:Cells, Cultured; D004655:Emulsions / Q000737:chemistry; D005260:Female; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism*; D011108:Polymers; D011388:Prolactin / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012685:Sepharose / Q000737:chemistry* / Q000633:toxicity
D000178:Acrylamides; D001672:Biocompatible Materials; D002214:Capsules; D004655:Emulsions; D011108:Polymers; D020106:Acrylamide; D011388:Prolactin; D012685:Sepharose