Enzyme immunoassay for (3-amino-1-methyl-5H-pyrido[4,3-b]indole), a tryptophan pyrolysate.
For evaluation of the cancer risk by the tryptophan pyrolysates a sensitive enzyme immunoassay for 3-amino-1-methyl-5H-pyrido-[4,3-b]indole (Trp-P-2), a mutagenic and carcinogenic substance, found in foods and biological fluids has been developed. The dose-response curve obtained was suitable for the determination of Trp-P-2 in the range of 20-800 pg. The immunoassay system was characterized to be satisfactorily specific as judged by cross-reactivity to gamma-carboline derivatives and their precursors, and comparison of immunoreactive Trp-P-2 levels in the basic and neutral fraction derived from pyrolyzed amino acids and related compounds.
Miyairi
S
S
Pharmaceutical Institute, Tohoku University, Sendai, Japan.
Nambara
T
T
D002243:Carbolines / Q000032:analysis*; D002273:Carcinogens / Q000032:analysis*; D006358:Hot Temperature; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D015394:Molecular Structure; D009153:Mutagens / Q000032:analysis*; D013638:Tars; D014364:Tryptophan; D001616:beta-Galactosidase / Q000032:analysis*
D002243:Carbolines; D002273:Carcinogens; D007202:Indicators and Reagents; D009153:Mutagens; D013638:Tars; C019133:3-amino-1-methyl-5H-pyrido(4,3-b)indole; D014364:Tryptophan; D001616:beta-Galactosidase