Coated hydrophilic polystyrene-based packing materials.
A very hydrophilic high-performance liquid chromatographic base support was created from microparticulate, macroporous poly(styrene-divinylbenzene) beads. An organic monomer containing cross-linking functionalities was coated on the poly(styrene-divinylbenzene), followed by a catalyzed cross-linking reaction. The coatings formed contain only stable chemical bonds (e.g., C-C, C-O-C), and easily-derivatized hydroxyl moieties. This coated base support was evaluated for hydrophilicity, chemical stability, solvent compatibility, rigidity, and irreversible adsorption. Derivatives of the coated base support were made and applied in various modes of chromatography.
Yang
Y B
YB
Department of Biochemistry, Purdue University, West Lafayette, IN 47907.
Regnier
F E
FE
D002412:Cations; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003432:Cross-Linking Reagents; D007475:Ion Exchange Resins; D008855:Microscopy, Electron, Scanning; D011137:Polystyrenes / Q000737:chemistry; D011506:Proteins / Q000032:analysis; D012116:Resins, Plant; D012997:Solvents; D014753:Vinyl Compounds
D002412:Cations; D003432:Cross-Linking Reagents; D007475:Ion Exchange Resins; D011137:Polystyrenes; D011506:Proteins; D012116:Resins, Plant; D012997:Solvents; D014753:Vinyl Compounds; C004985:divinyl benzene