[Value and limitations of anti-mannan antibodies research by co-immunoelectrodiffusion in the diagnosis of deep candidiasis].
Several groups have evaluated detection of antibodies against Candida, with somewhat conflicting results. In this study, co-counterimmunoelectrodiffusion was used to detect antimannan antibodies specific of components of the Candida membrane. Study patients were divided into two groups according to whether their history for Candida infection was negative (population A, n = 102) or positive (population B). Different antigen levels were used in order to differentiate low and high antimannan antibody levels. Among the 102 sera in population A, 42 were positive for antimannan antibodies; the antimannan antibody titer was low in 40 cases and high in 2 cases. In population B (53 patients), antimannan antibodies were found in 97 of the 98 sera studied; titers were high in 95 cases. Use of an antigen level that detects only high titers of antimannan antibodies thus provides a sensitive and specific tool for the diagnosis of deep candidiasis. The simplicity and rapidity of this test are particularly valuable in situations where emergency treatment is needed.
Puygauthier-Toubas
D
D
Laboratoire de Parasitologie-Mycologie, Hôpital Maison Blanche, CHRU, Reims, France.
Pailler
S
S
Marx-Chemla
C
C
Léon
A
A
Pignon
B
B
Bonnin
A
A
Spehner
V
V
Pinon
J M
JM
D000906:Antibodies / Q000032:analysis*; D002177:Candidiasis / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion / Q000379:methods*; D007122:Immunoelectrophoresis / Q000379:methods*; D008351:Mannans / Q000276:immunology*; D012680:Sensitivity and Specificity
D000906:Antibodies; D008351:Mannans
Intérêt et limites de la recherche des anticorps anti-mannane par co-immunoélectrodiffusion dans le diagnostic des candidoses profondes.