title: Effect of increased Hb-O2 affinity on VO2max at constant O2 delivery in dog muscle in situ.
abstract: We investigated the effect of increasing hemoglobin- (Hb) O2 affinity on muscle maximal O2 uptake (VO2max) while muscle blood flow, [Hb], HbO2 saturation, and thus O2 delivery (muscle blood flow X arterial O2 content) to the working muscle were kept unchanged from control. VO2max was measured in isolated in situ canine gastrocnemius working maximally (isometric tetanic contractions). The muscles were pump perfused, in alternating order, with either normal blood [O2 half-saturation pressure of hemoglobin (P50) = 32.1 +/- 0.5 (SE) Torr] or blood from dogs that had been fed sodium cyanate (150 mg.kg-1.day-1) for 3-4 wk (P50 = 23.2 +/- 0.9). In both conditions (n = 8) arterial PO2 was set at approximately 200 Torr to fully saturate arterial blood, which thereby produced the same arterial O2 contents, and muscle blood flow was set at 106 ml.100 g-1.min-1, so that O2 delivery in both conditions was the same. VO2max was 11.8 +/- 1.0 ml.min-1.100 g-1 when perfused with the normal blood (control) and was reduced by 17% to 9.8 +/- 0.7 ml.min-1.100 g-1 when perfused with the low-P50 blood (P less than 0.01). Mean muscle effluent venous PO2 was also significantly less (26 +/- 3 vs. 30 +/- 2 Torr; P less than 0.01) in the low-P50 condition, as was an estimate of the capillary driving pressure for O2 diffusion, the mean capillary PO2 (45 +/- 3 vs. 51 +/- 2 Torr). However, the estimated muscle O2 diffusing capacity was not different between conditions.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Medicine, University of California, San Diego, La Jolla 92093-0623.
authors.forename: M C
authors.initials: MC
authors.lastname: Hogan
authors.forename: D E
authors.initials: DE
authors.lastname: Bebout
authors.forename: P D
authors.initials: PD
authors.lastname: Wagner
mesh_terms: D000136:Acid-Base Equilibrium; D000818:Animals; D003485:Cyanates / Q000633:toxicity; D004058:Diffusion; D004285:Dogs; D005260:Female; D006454:Hemoglobins / Q000378:metabolism*; D007537:Isometric Contraction / Q000502:physiology; D008297:Male; D009132:Muscles / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D012039:Regional Blood Flow
chemical_list: D003485:Cyanates; D006454:Hemoglobins; C009281:sodium cyanate; D010100:Oxygen