A monoclonal antibody (OPT1) to T cells which is available for paraffin-embedded materials.
A monoclonal antibody (MAb), OPT1, reactive with T cells in formalin-fixed, paraffin-embedded tissue sections, has been identified through immunization with activated T cells from peripheral blood lymphocytes (PBL). The antibody is an IgG1 antibody as demonstrated by the Ouchterlony technique. By cytofluorometric analysis, almost all CD3+ lymphocytes and only a few CD20+ lymphocytes of peripheral blood expressed the OPT1 antigen. Nonhematolymphoid cell lines were negative for OPT1 by the immunoperoxidase staining using acetone-fixed cell lines. On the contrary, peripheral T cells, cells of two T cell lines out of four and a part of the cells of one B cell line out of two were positive for OPT1. The immunoperoxidase staining of paraffin-embedded tissue sections revealed that most of lymphocytes in T cell areas of lymph nodes expressed OPT1 antigen. Some lymphocytes in both cortex and medulla of the thymus and erythroid precursors of the bone marrow were OPT1+. In the malignant lymphoma series, approximately 90% of T cell lymphomas and 6% of B cell lymphomas reacted with OPT1. None of the Reed-Sternberg cells nor Hodgkin cells in Hodgkin's disease were positive. Consequently, OPT1 may be useful for the diagnosis and study of malignant lymphomas and other related lesions.
Mukuzono
H
H
Second Department of Pathology, Okayama University Medical School, Japan.
Yoshino
T
T
Akagi
T
T
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001773:Blood Cells / Q000276:immunology; D002460:Cell Line; D005434:Flow Cytometry; D006652:Histological Techniques; D006801:Humans; D008221:Lymphoid Tissue / Q000276:immunology; D008223:Lymphoma / Q000276:immunology; D010232:Paraffin; D012016:Reference Values; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D010232:Paraffin