Analysis of age-associated alteration in the synthesis of HMG nonhistone proteins of the rat liver.
HMG proteins were extracted with 5% PCA or 0.35 M NaCl from whole tissue, nuclei or chromatin of the liver of young (19 weeks) and old (118 weeks) male rats. They were resolved on acetic acid-urea polyacrylamide gel. The electrophoretic patterns of the major HMG proteins 1, 2, 14 and 17 of both ages are similar. The in vitro synthesis of HMG 1 and 2 decreases, but that of HMG 14 and 17 increases considerably in the liver of old rats. The synthesis of different HMG proteins is modulated differentially by spermine, butyrate, dexamethasone and 3-aminobenzamide in the liver of young and old rats. These findings suggest that HMG proteins contribute to alterations in the organization of chromatin and expression of genes during aging.
Thakur
M K
MK
Biochemistry and Molecular Biology Laboratory, Banaras Hindu University, Varanasi, India.
Prasad
S
S
D000375:Aging; D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D003907:Dexamethasone / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006609:High Mobility Group Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013096:Spermine / Q000494:pharmacology
D001549:Benzamides; D002087:Butyrates; D006609:High Mobility Group Proteins; D020148:Butyric Acid; D013096:Spermine; D003907:Dexamethasone; C025160:3-aminobenzamide; D008239:Lysine