title: Regulation of the human T-cell response to Schistosoma japonicum egg antigen by concomitant cellular and humoral mechanisms in vitro.
abstract: Serum-mediated regulation of T-cell responses specific for soluble egg antigen (SEA) of Schistosoma japonicum was tested in human hosts. When we added autologous serum to SEA-specific human T-cell lines (CD3+, 4+, 8-), we observed suppression of T-cell proliferation, and this suppressive activity was detected in the immunoglobulin-G2 (IgG2) subclass. Suppression was dose-dependent and antigen-specific. T-cell proliferation induced by only one SEA fraction of greater than 18 kDa was modulated in the presence of 100 micrograms/ml autologous as well as allogeneic infected IgG2. This SEA fraction-driven proliferation was also regulated by suppressor T-cells through distinct suppressive mechanisms. Our results suggest that T-cell responses to a particular component(s) of SEA are strictly regulated through both cellular and humoral mechanisms in human chronic S. japonicum infection.
authors.affiliation: Department of Parasitology, Okayama University Medical School, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ohta
authors.forename: H
authors.initials: H
authors.lastname: Asahi
authors.forename: Y
authors.initials: Y
authors.lastname: Hosaka
authors.forename: M
authors.initials: M
authors.lastname: Minai
authors.forename: A
authors.initials: A
authors.lastname: Ishii
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D010063:Ovum / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G