title: [Proteolytic activity of the Glu-plasminogen complex with fibrinogen fragment E].
abstract: Glu-plasminogen interaction with fibrinogen fragment E results in the alteration of its adsorptive capacity. During this interaction in the absence of plasmin and tissue activator of plasminogen, Glu-plasminogen is transformed into a partly degraded form. Glu-plasminogen complexes with soluble and immobilized fibrinogen fragment E. contain a serine proteinase-specific activity which is inhibited by diisopropylfluorophosphate. The complexes under study are active towards fibrin and the plasmin-specific tripeptide substrate, D-Val-L-Leu-L-Lys-p-nitroanilide. It is concluded that fibrinogen fragment E induces structural changes in the enzyme molecule which eventually result in the formation of an active center.
authors.forename: E V
authors.initials: EV
authors.lastname: Skomorovskaia
authors.forename: T V
authors.initials: TV
authors.lastname: Grinenko
authors.forename: S A
authors.initials: SA
authors.lastname: Kudinov
authors.forename: E N
authors.initials: EN
authors.lastname: Zolotareva
mesh_terms: D001665:Binding Sites; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism*; D005342:Fibrinolysis; D006801:Humans; D006868:Hydrolysis; D010958:Plasminogen / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000378:metabolism
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; C018543:fibrinogen fragment E; D010958:Plasminogen; D012697:Serine Endopeptidases
vernacular_title: Issledovanie proteolitcheskoĭ aktivnosti kompleksa Glu-plasminogena s fragmentom E fibrinogena.