Antibody against neurofibromatosis type 1 gene product reacts with a triton-insoluble GTPase activating protein toward ras p21.
Cellular fractionation of GTPase activating protein (GAP) activity using bovine cerebral cortex revealed that about half of GAP activity was found in membrane fraction. GAP activity of membrane was not solubilized with 0.5% (v/v) triton X-100 and was immunoprecipitated with antibody against carboxy-terminus of neurofibromatosis type 1 (NF1) gene product. In contrast, soluble GAP activity was precipitated with antibody against GAP but not with anti-NF1. These results suggest that NF1 gene product is a GTPase activating protein toward ras p21 with completely different intracellular distribution from that of GAP.
Hattori
S
S
Division of Biochemistry and Cellular Biology, National Institute of Neuroscience, Tokyo, Japan.
Ohmi
N
N
Maekawa
M
M
Hoshino
M
M
Kawakita
M
M
Nakamura
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D002417:Cattle; D002540:Cerebral Cortex / Q000378:metabolism*; D003902:Detergents; D020690:GTPase-Activating Proteins; D006801:Humans; D008969:Molecular Sequence Data; D009456:Neurofibromatosis 1 / Q000235:genetics; D025542:Neurofibromin 1; D017830:Octoxynol; D015689:Oncogene Protein p21(ras) / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis; D011092:Polyethylene Glycols; D011506:Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D012995:Solubility; D020703:ras GTPase-Activating Proteins
D000906:Antibodies; D003902:Detergents; D020690:GTPase-Activating Proteins; D025542:Neurofibromin 1; D010455:Peptides; D011506:Proteins; D020703:ras GTPase-Activating Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; D015689:Oncogene Protein p21(ras)