The effect of vitamin D on rat intestinal plasma membrane CA-pump mRNA.
The effects of vitamin D on steady-state levels of rat intestinal Ca-pump mRNA were examined in RNA extracted from isolated cell fractions of the crypt-to-villus gradient of differentiation. Northern blots revealed three different size mRNAs. Vitamin D deficient animals showed a decrease in these Ca-pump mRNAs, which increased markedly after 1,25-(OH)2D3 repletion, particularly for the villus cell. The data suggest that one of the effects of 1,25-(OH)2D3 may be to modulate enterocyte Ca-pump mRNA and that this effect is partly dependent on the stage of cell differentiation.
Zelinski
J M
JM
Department of Medicine, State University of New York, Buffalo.
Sykes
D E
DE
Weiser
M M
MM
D000818:Animals; D001693:Biological Transport, Active; D015152:Blotting, Northern; D002117:Calcitriol / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane; D015342:DNA Probes; D007421:Intestine, Small / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008871:Microvilli / Q000378:metabolism / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D014808:Vitamin D Deficiency / Q000503:physiopathology*
D015342:DNA Probes; D012333:RNA, Messenger; D002117:Calcitriol; D002118:Calcium