Application of an ovomucoid-conjugated polymer column for the enantiospecific determination of chlorprenaline concentrations in plasma.
An ovomucoid-conjugated polymer column was prepared for the liquid chromatographic resolution of racemic compounds. The column showed strong retention of acidic solutes, a characteristic attributed to the structure of the stationary phase support gel. Although the efficiency of the column was lower than that of an ovomucoid-conjugated silica gel column, enantiospecific chlorprenaline determination in plasma was achieved with solute amounts from 1.0 ng to 0.1 microgram.
Miwa
T
T
Eisai Co., Ltd., Pharmaceutical Research Laboratory, Gifu, Japan.
Sakashita
S
S
Ozawa
H
H
Haginaka
J
J
Asakawa
N
N
Miyake
Y
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D000431:Ethanol; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007545:Isoproterenol / Q000031:analogs & derivatives* / Q000097:blood; D008832:Microchemistry; D010059:Ovomucin; D011108:Polymers; D013237:Stereoisomerism
D011108:Polymers; D010059:Ovomucin; D000431:Ethanol; C004350:clorprenaline; D007545:Isoproterenol