Both D factor/LIF and IL-6 inhibit the differentiation of mouse teratocarcinoma F9 cells.
Differentiation-stimulating factor (D factor)/leukemia inhibitory factor (LIF) and IL-6 are reported to be cytokines having multifaced functions including the induction of differentiation in mouse myeloid leukemia M1 cells. We here report that both D factor/LIF and IL-6 inhibit the differentiation of mouse teratocarcinoma F9 cells induced by retinoic acid alone or combined with dibutyryl cAMP. From the microscopic observation as well as Northern blot analysis using cDNA probes encoding several marker proteins for differentiation of F9 cells, we concluded that D factor/LIF and IL-6 are functionally closely related in the induction of differentiation in M1 cells and in the inhibition of F9 differentiation.
Hirayoshi
K
K
Department of Cell Biology, University of Kyoto, Japan.
Tsuru
A
A
Yamashita
M
M
Tomida
M
M
Yamamoto-Yamaguchi
Y
Y
Yasukawa
K
K
Hozumi
M
M
Goeddel
D V
DV
Nagata
K
K
D000469:Alkaline Phosphatase / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D002454:Cell Differentiation / Q000187:drug effects*; D003094:Collagen / Q000235:genetics; D015870:Gene Expression; D006131:Growth Inhibitors; D066298:In Vitro Techniques; D015850:Interleukin-6 / Q000494:pharmacology*; D007797:Laminin / Q000235:genetics; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology*; D051379:Mice; D015676:Osteonectin / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D013724:Teratoma / Q000473:pathology; D014407:Tumor Cells, Cultured
D006131:Growth Inhibitors; D015850:Interleukin-6; D007797:Laminin; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D015676:Osteonectin; D012333:RNA, Messenger; D003094:Collagen; D000469:Alkaline Phosphatase