An unsulphated region of the rat chondrosarcoma chondroitin sulphate chain and its binding to monoclonal antibody 3B3.
The chondroitin sulphate chains of proteoglycans are not uniformly sulphated. Commonly, regions of under- and over-sulphation are found. It is probable that variability in chondroitin sulphation has physiological significance, although such structure-function relationships largely remain unexplored. Chondroitin sulphate from rat chondrosarcoma proteoglycan has been found to possess no oversulphated residues. It is primarily chondroitin 4-sulphate, although a significant proportion of unsulphated disaccharides (14%) are also present. It appears that some unsulphated disaccharides are concentrated only at the point of attachment to the linkage region (i.e. it is the major unsaturated disaccharide remaining attached to chondrosarcoma proteoglycan core produced by chondroitinase ABC digestion). This proteoglycan core binds monoclonal antibody (MAb) 3B3. Although 3B3 principally binds to 6-sulphated 'stubs' of proteoglycan cores [Couchman, Caterson, Christner & Baker (1984) Nature (London) 307, 650-652], given a high concentration of unsulphated 'stubs', it can alternatively bind to these residues. It is also evident that caution must be exercised in using MAb 3B3 to identify chondroitin 6-sulphated proteoglycans.
Baker
J R
JR
Department of Biochemistry, University of Alabama, Birmingham 35294.
Christner
J E
JE
Ekborg
S L
SL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000276:immunology; D002810:Chondroitinases and Chondroitin Lyases; D002813:Chondrosarcoma / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D015151:Immunoblotting; D009844:Oligosaccharides / Q000032:analysis; D051381:Rats; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D009844:Oligosaccharides; D002809:Chondroitin Sulfates; D002810:Chondroitinases and Chondroitin Lyases