Purification of the protein X of Streptococcus agalactiae with a monoclonal antibody.
The protein X of Steptococcus agalactiae is a surface antigen included in the typing scheme of group B streptococci (GBS). We have developed a monoclonal antibody to the protein X and used it to purify this antigen by affinity chromatography. Electrophoresis in polyacrylamide, and immunoblotting using the monoclonal antibody or a rabbit antiserum raised with the affinity purified protein X, revealed a major band in the region of 200 kDa and a smaller one at 100 kDa. The isolated protein X will make possible investigations of its potential role in virulence and protection.
Lautrou
Y
Y
Institut National de la Recherche Agronomique, Laboratoire de Pathologie Infectieuse et Immunologie, Nouzilly, France.
Rainard
P
P
Poutrel
B
B
Zygmunt
M S
MS
Vénien
A
A
Dufrenoy
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification*; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D008414:Mastitis, Bovine / Q000382:microbiology; D051379:Mice; D011817:Rabbits; D013292:Streptococcus agalactiae / Q000032:analysis*
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins