Enhancement of murine susceptibility to oral lactate dehydrogenase-elevating virus infection by non-steroidal anti-inflammatory agents, and antagonism by misoprostol.
The murine lactate dehydrogenase-elevating virus (LDV) was used to study the effects of prostaglandin-acting agents on mucosal resistance to virus infection. Mice treated with non-steroidal anti-inflammatory drugs (NSAIDs) prior to oral exposure to LDV demonstrated a reduction in the mucosal barrier to LDV infection. Histological studies indicated that these NSAID effects were not a result of gross or microscopic tissue damage. The effects of two NSAIDs, indomethacin and diclofenac, were inhibited by co-treatment of mice with misoprostol, a synthetic PGE1 analog. The ability of misoprostol to modulate NSAID effects was not due to direct antiviral activity or to actions on LDV-permissive macrophages. These results show that the mammalian mucosal barrier to virus infection is prostaglandin-sensitive, and provide a model for the study of resistance to viral infection.
Cafruny
W A
WA
Department of Microbiology, University of South Dakota, Vermillion 57069.
DesJarlais
S E
SE
Hecht
M L
ML
Broen
J J
JJ
Jaqua
R A
RA
D000527:Alprostadil / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D001241:Aspirin / Q000494:pharmacology; D004008:Diclofenac / Q000494:pharmacology; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D007772:Lactate dehydrogenase-elevating virus / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D016595:Misoprostol; D014777:Virus Diseases / Q000188:drug therapy
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000998:Antiviral Agents; D016595:Misoprostol; D004008:Diclofenac; D000527:Alprostadil; D001241:Aspirin; D007213:Indomethacin