[Long-term induced effects by only one treatment with ketoconazole on rat tumoral pituitary cells (GH3/B6 strains)].
Ketoconazole, an imidazole is a powerful antimycotic that has recently been used in the treatment of endocrinological and lipid metabolism disorders as well as in anti-cancer chemotherapy. When rat mammosomatotropic cells are treated for 30 hrs., this drug produces different effects depending on whether cells are normal or tumoral (GH3/B6). A dose of 10 microM had no effect in normal cells, however, in tumoral cells it had dramatic effects: (i) 50% of the cells are killed and those which survive no longer proliferate and they secrete GH but not PRL; (ii) they respond to GHRH in a dose-dependent manner, while normal cells do not under similar culture conditions; (iii) the lipid composition of the membrane is modified as indicated by the increase in arachidonic acid turn-over and the dramatic change in the distribution of its metabolites. For the moment we cannot explain these data.
Drouhault
R
R
Laboratoire de Neurophysiologie, C.N.R.S.-U.R.A. n 1200, Université de Bordeaux-II.
Vacher
P
P
Darret
D
D
Larrue
J
J
Vacher
A M
AM
Vilayleck
N
N
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D004594:Electrophysiology; D013007:Growth Hormone-Releasing Hormone / Q000494:pharmacology; D007654:Ketoconazole / Q000494:pharmacology*; D010902:Pituitary Gland / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D010911:Pituitary Neoplasms / Q000473:pathology* / Q000503:physiopathology; D051381:Rats; D011919:Rats, Inbred Strains; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology
D001095:Arachidonic Acids; D013973:Thyrotropin-Releasing Hormone; D013007:Growth Hormone-Releasing Hormone; D007654:Ketoconazole
Effets induits à long terme par un seul traitement de kétoconazole sur des cellules tumorales hypophysaires de rat (lignée GH3/B6).