Effect of anti-Ig on rabbit B cell function and Ig expression.
We have examined the effect which xenogeneic anti-Ig has on rabbit B cell function and Ig expression in an effort to understand the phenomenon of antibody mediated suppression. Treatment of rabbit lymphocyte cultures with xenogeneic anti-rabbit Ig causes 2.5-3.8 fold decrease in the level of Ig secreting cells with little or no long-term effect on surface Ig. This suppression in B cell secretory function is not the result of suppression of Ig gene expression since xenogeneic anti-rabbit Ig treatment causes a 1.7-2.7 fold increase in Ig L and H chain mRNA levels. Collectively, these data are consistent with the hypothesis that antibody mediated suppression of B cell function occurs at a post-transcriptional level involving either the secretory pathway of Ig expression and/or blockage in B cell differentiation.
Ives
N
N
Department of Pathology, Geisinger Medical Center, Danville, PA 17822.
Asundi
V
V
Dreher
K
K
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology*; D000910:Antibodies, Heterophile / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology*; D015152:Blotting, Northern; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007075:Immunoglobulin M / Q000096:biosynthesis*; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D013154:Spleen / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000910:Antibodies, Heterophile; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D012333:RNA, Messenger