Quantitative determination of albumin in urine by on-line immunoadsorptive cleanup and reversed-phase chromatography.
Albumin in urine is selectively adsorbed on an immunoadsorber (human serum albumin-specific antibodies coupled covalently with a silica stationary phase) and after elution with 0.1% HCl is quantitatively determined by reversed-phase chromatography with detection of native fluorescence. The optimization of sample preparation and characteristics of the method such as recovery, linearity, reproducibility, detection limit, and selectivity are discussed.
Reh
E
E
Boehringer-Mannheim GmbH, Department DB-VA, Germany.
Kratzer
M
M
Zdunek
D
D
Lang
F
F
D000419:Albuminuria; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007163:Immunosorbent Techniques; D015203:Reproducibility of Results