Assembly of empty capsids by using baculovirus recombinants expressing human parvovirus B19 structural proteins.
Empty parvovirus B19 capsids were isolated from insect cells infected with a recombinant baculovirus expressing parvovirus B19 VP2 alone and also with a double-recombinant baculovirus expressing both VP1 and VP2. That VP2 alone can assemble to form capsids is a phenomenon not previously observed in parvoviruses. The stoichiometry of the capsids containing both VP1 and VP2 was similar to that previously observed in parvovirus B19-infected cells. The capsids were similar to native capsids in size and appearance, and their antigenicity was demonstrated by immunoprecipitation and enzyme-linked immunosorbent assay with B19-specific antibodies.
Brown
C S
CS
Department of Virology, Faculty of Medicine, Leiden University, The Netherlands.
Van Lent
J W
JW
Vlak
J M
JM
Spaan
W J
WJ
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002213:Capsid / Q000096:biosynthesis* / Q000235:genetics; D036022:Capsid Proteins; D002460:Cell Line; D004274:DNA, Recombinant; D004331:Drosophila melanogaster; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009036:Moths; D010321:Parvoviridae / Q000235:genetics* / Q000378:metabolism; D011233:Precipitin Tests; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D015678:Viral Structural Proteins / Q000096:biosynthesis* / Q000235:genetics
D036022:Capsid Proteins; D004274:DNA, Recombinant; D011994:Recombinant Proteins; D015678:Viral Structural Proteins