A physical linkage map of HLA-A, -G, -7.5p, and -F.
The class I region of the human leukocyte antigen (HLA) complex includes genes encoding the classical transplantation antigens (HLA-A, -B, -C), at least three nonclassical class I genes (HLA-E, -F, and -G), and many class I pseudogenes (including HLA-7.5p). We have used probes from DNA within or flanking the HLA -A, -F, -G, and -7.5p genes to construct a physical linkage map that places the HLA-F, -G, and -7.5p loci in order with respect to HLA-A. The map was constructed using clamped homogeneous electric field pulsed-field gel electrophoresis. DNA was isolated from LCL 721 (A1:B8, A2:B5), a human Epstein-Barr virus-transformed lymphoblastoid cell line (LCL), and from two gamma-irradiation-induced mutants of LCL 721 lacking complementary class I haplotypes. The physical linkage data place HLA-G closest to HLA-A and place HLA-7.5p between HLA-G and HLA-F. The map constructed supports a maximum distance of 490 kilobases between HLA-A and HLA-F.
Schmidt
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CM
Department of Laboratory Medicine, University of Minnesota, Minneapolis 55455.
Orr
H T
HT
D002461:Cell Line, Transformed; D002874:Chromosome Mapping; D005805:Genes, MHC Class I; D008040:Genetic Linkage; D006680:HLA Antigens / Q000235:genetics*; D015234:HLA-A Antigens / Q000235:genetics*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D008285:Major Histocompatibility Complex; D011544:Pseudogenes; D015183:Restriction Mapping
D006680:HLA Antigens; D015234:HLA-A Antigens; C062341:HLA-F antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I