title: Correlation between mRNA levels for bone cell proteins and bone formation in long bones of maturing rats.
abstract: This report describes the relationship between bone formation and mRNA levels for selected bone proteins. Dynamic bone histomorphometry was used to measure bone formation in tibial periosteum of male rats from weanling (3 wk) to 52 wk old. Northern blot analysis of freshly isolated periosteal cells from the long bones was used to determine steady-state mRNA levels for the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAP), the bone matrix proteins osteocalcin (BGP), and prepro-alpha-2 (I) chain of type 1 precollagen (collagen), the osteoblast marker enzyme alkaline phosphatase (AP), and the osteoblast-derived signaling factor (growth factor) transforming growth factor-beta (TGF-beta). Radial growth at the tibial diaphysis achieved a maximum value in 8-wk-old rats and decreased progressively with age thereafter. This age-related decrease in the radial growth rate was initially due to reduced osteoblast activity; however, in older rats (greater than 17 wk old) reduced osteoblast number contributed to the decrease in bone formation. There was a strong correlation between the steady-state mRNA level for collagen and the periosteal bone formation rate. In contrast, the mRNA levels for the other bone proteins were more weakly correlated (TGF-beta and AP) or not correlated (BGP). These results suggest that the decreased bone matrix synthesis by periosteal cells in long bones of maturing rats is due to decreased expression of genes for bone matrix proteins.
authors.affiliation: Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota 55905.
authors.forename: R T
authors.initials: RT
authors.lastname: Turner
authors.forename: T C
authors.initials: TC
authors.lastname: Spelsberg
mesh_terms: D000375:Aging; D000469:Alkaline Phosphatase / Q000235:genetics*; D000818:Animals; D001846:Bone Development; D001842:Bone and Bones / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology; D015342:DNA Probes; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D010006:Osteoblasts / Q000502:physiology*; D015675:Osteocalcin / Q000235:genetics*; D011347:Procollagen / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D016212:Transforming Growth Factor beta / Q000235:genetics*
chemical_list: D015342:DNA Probes; D007527:Isoenzymes; D011347:Procollagen; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D015675:Osteocalcin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D000469:Alkaline Phosphatase