Inhibition of intracellular growth of Histoplasma capsulatum yeast cells by cytokine-activated human monocytes and macrophages.
Human monocytes/macrophages (M psi) were infected with Histoplasma capsulatum yeast cells, and intracellular growth was quantified after 24 h of incubation in medium alone or in medium containing cytokines. Yeast cells multiplied within freshly isolated monocytes, cultured M psi, and alveolar M psi with intracellular generation times of 14.2 +/- 1.4, 18.5 +/- 2.1, and 19.9 +/- 1.9 h (mean +/- standard error of the mean), respectively. Monocytes and M psi inhibited the intracellular growth of yeast cells in response to cytokine supernatant; maximum inhibition was obtained when cytokines were added to cell monolayers immediately after infection. Opsonization of yeast cells in normal serum or in H. capsulatum-immune serum did not affect the intracellular generation time of yeast cells in either control M psi or cytokine-activated M psi.
Newman
S L
SL
Department of Medicine, University of Cincinnati College of Medicine, Ohio 45267.
Gootee
L
L
Bucher
C
C
Bullock
W E
WE
D016207:Cytokines / Q000494:pharmacology*; D006658:Histoplasma / Q000254:growth & development*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000276:immunology / Q000382:microbiology*; D009000:Monocytes / Q000276:immunology / Q000382:microbiology*; D010587:Phagocytosis
D016207:Cytokines; D007371:Interferon-gamma