Improvement of a Vero cell assay to determine diphtheria antitoxin content in sera.
Diphtheria antitoxin content in sera were determined automatically in Vero cell assay by spectrophotometric determination of the equivalence point between toxin and antitoxin followed by computer analysis of absorption values. The method was more accurate than visual reading and made handling of many samples easy.
Aggerbeck
H
H
State Serum Institute, Bacteria Vaccine Department, Copenhagen S, Denmark.
Heron
I
I
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D003116:Color; D004166:Diphtheria Antitoxin / Q000097:blood* / Q000592:standards; D004167:Diphtheria Toxin; D006801:Humans; D051379:Mice; D013053:Spectrophotometry; D014709:Vero Cells
D004166:Diphtheria Antitoxin; D004167:Diphtheria Toxin