[Prevention of myocardial reperfusion injury by increasing artificial trans-membrane sodium gradient in "calcium paradox" and post-ischemic reperfusion].
An effect of the high sodium gradient during "calcium paradox" and postischemic reperfusion has been studied. A decrease of Na/Ca exchange by high sodium gradient (200 mM NaCl in the perfusion solution) resulted in the reduction of myoglobin release from the heart during "calcium paradox". High sodium concentration solution (200 mM) increased protective effect of ATP during "calcium paradox". Exogenous phosphocreatine (100 mumol/mol) increased myoglobin release from the heart. During perfusion of the heart by high sodium concentration, phosphocreatine efficiently decreased myoglobin release from the heart during "calcium paradox". Exogenous ATP (as Na-pump activator) and high Na+ concentration solution (180 mM) prevented the LDH release from the myocardium, decreased ATP hydrolysis, inhibited Ca influx, maintained total adenine nucleotides, phosphate potential, energy charge of the cardiomyocytes.
Alabovskiĭ
V V
VV
Vinokurov
A A
AA
Kobrin
V I
VI
Oleĭnikov
O D
OD
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000008:administration & dosage* / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004734:Energy Metabolism / Q000502:physiology*; D008955:Models, Cardiovascular; D015425:Myocardial Reperfusion / Q000379:methods*; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism*; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D012965:Sodium Chloride / Q000008:administration & dosage* / Q000494:pharmacology
D000227:Adenine Nucleotides; D010725:Phosphocreatine; D012965:Sodium Chloride; D002118:Calcium
Preduprezhdenie iskusstvennym uvelicheniem transmembrannogo gradients natriia reperfuzionnykh povrezhdeniĭ cerdtsa pri "kal'tsievom paradokse" i postishemicheskoĭ reperfuzii.