[Cloning and cytogenetic localization of evolutionarily conserved genomic sequences which are expressed in the head of the Drosophila melanogaster imago].
Screening of Drosophila melanogaster genomic library was carried out using mouse brain polysomal poly(A)+RNA. As a result, 100 clones were selected, among which 14 clones were picked up after hybridization with fly head poly(A)+RNA. It follows therefore, that these clones contain evolutionary conserved sequences which are expressed in Drosophila fly heads. Analysis of these 14 clones revealed RNA-coding fragments. Comparison of their expression in heads and bodies of Drosophila was carried out. Using in situ hybridization we determined the localization of selected 14 sequences on polytene chromosomes. The possibility of further analysis of some clones to study developmental and functional processes in neural system of Drosophila is discussed.
Kokoza
V A
VA
Baricheva
E M
EM
Katokhin
A V
AV
D000818:Animals; D001345:Autoradiography; D005075:Biological Evolution; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D005786:Gene Expression Regulation; D007621:Karyotyping; D009693:Nucleic Acid Hybridization
D004247:DNA
Klonirovanie i tsitogeneticheskaia lokalizatsiia évoliutsionno konservativnykh, ékspressiruiushchikhsia v golovakh imago genomnykh posledovatel'nosteĭ Drosophila melanogaster.