title: Acanthamoeba keratitis: experience in a non-specialist microbiology laboratory.
abstract: Acanthamoeba keratitis is an unusual complication of wearing soft contact lenses: a case was diagnosed by culturing corneal scrapings. Acanthamoeba are tolerant of variations in osmolarity in the culture media. Page's saline, recommended for culture agar plates, can easily be substituted with salines which are suitable for cell culture media, thereby avoiding the cumbersome manufacture of special saline. Early diagnosis as a result of successful isolation may avoid the need for keratoplasty.
authors.affiliation: Public Health Laboratory, West Norwich Hospital, Norfolk.
authors.forename: D
authors.initials: D
authors.lastname: Anderson
authors.forename: S S
authors.initials: SS
authors.lastname: Soo
authors.forename: H
authors.initials: H
authors.lastname: Towler
mesh_terms: D000048:Acanthamoeba / Q000302:isolation & purification; D015823:Acanthamoeba Keratitis / Q000175:diagnosis* / Q000209:etiology / Q000469:parasitology; D000328:Adult; D000818:Animals; D019411:Clinical Laboratory Techniques; D003263:Contact Lenses, Hydrophilic / Q000009:adverse effects; D003315:Cornea / Q000469:parasitology; D003470:Culture Media; D006801:Humans; D008297:Male; D012965:Sodium Chloride
chemical_list: D003470:Culture Media; D012965:Sodium Chloride