A simple handling technique for mammalian oocytes and embryos during preparation for transmission electron microscopy.
Due to their small size, mammalian oocytes and embryos pose unique problems during preparation for transmission electron microscopy. This paper outlines a method which combines protein embedding with centrifugation to locate the specimens on the face of a Beem capsule mould. This method facilitates both the processing of oocytes with minimal loss and rapid location of the specimens within the block for simultaneous sectioning, staining and examination.
Britton
A P
AP
Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, Canada.
Moon
Y S
YS
Yuen
B H
BH
D000818:Animals; D002498:Centrifugation; D004622:Embryo, Mammalian / Q000648:ultrastructure*; D005260:Female; D005976:Glutaral; D006652:Histological Techniques; D008854:Microscopy, Electron; D009865:Oocytes / Q000648:ultrastructure*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains
D005976:Glutaral