Transcription of the Bacillus subtilis spoIIA locus.
The spoIIA operon encodes three genes, including the structural gene for a sporulation-induced sigma factor sigma F. We used deletion analysis of spoIIA-lacZ fusions to define the location of the spoIIA promoter. We found that sigma H-RNA polymerase transcribes spoIIA accurately in vitro and propose that sigma H directs transcription of spoIIA during sporulation.
Wu
J J
JJ
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140.
Piggot
P J
PJ
Tatti
K M
KM
Moran
C P
CP
D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000502:physiology; D012808:Sigma Factor / Q000502:physiology; D013171:Spores, Bacterial; D014157:Transcription Factors; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis
D001426:Bacterial Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus; D001616:beta-Galactosidase