title: An inhibition enzyme immunoassay using a human monoclonal antibody (K14) reactive with gp41 of HIV-1 for the serology of HIV-1 infections.
abstract: An inhibition enzyme immunoassay (IEIA), using a human monoclonal antibody (K14) reactive with gp41 of HIV-1, was evaluated for its applicability to the serology of HIV-1 infections. Using panels of serum samples from seronegative and confirmed HIV-1-seropositive individuals, it was shown that all the HIV-1-positive samples in a panel from The Netherlands and 97% of the HIV-1-positive samples from Tanzania were identified by this IEIA. Six per cent of the IEIA-positive samples from Tanzania could not be confirmed in other assays. Testing of serial dilutions of serum samples from African individuals with confirmed HIV-1, HIV-2 or HIV(ANI70) infections in the K14 IEIA, indicated that a HIV-1-specific assay based on this principle may be developed.
authors.affiliation: Laboratory of Immunobiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
authors.forename: V J
authors.initials: VJ
authors.lastname: Teeuwsen
authors.forename: J J
authors.initials: JJ
authors.lastname: Schalken
authors.forename: G
authors.initials: G
authors.lastname: van der Groen
authors.forename: R
authors.initials: R
authors.lastname: van de Akker
authors.forename: J
authors.initials: J
authors.lastname: Goudsmit
authors.forename: A D
authors.initials: AD
authors.lastname: Osterhaus
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D005069:Evaluation Studies as Topic; D015700:HIV Envelope Protein gp41 / Q000276:immunology*; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques
chemical_list: D000911:Antibodies, Monoclonal; D015700:HIV Envelope Protein gp41