Simultaneous determination of trimipramine and its major metabolites by high-performance liquid chromatography.
Trimipramine is a tricyclic antidepressant drug often assayed by gas chromatographic or gas chromatographic-mass spectrometry techniques. A high-performance liquid chromatographic method with electrochemical detection is described for the assay of trimipramine and its major metabolites, monodesmethyltrimipramine and 2-hydroxytrimipramine, in plasma. The method is sensitive, accurate and robust and thus suitable for routinely assaying samples following single doses of trimipramine to man. The assay was applied to plasma samples obtained following a single 50-mg dose of trimipramine to healthy volunteers.
Gulaid
A A
AA
Biopharmacy Department, Rhône-Poulenc Rorer, Ltd., Dagenham, Essex, U.K.
Jahn
G A
GA
Maslen
C
C
Dennis
M J
MJ
D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D008297:Male; D015394:Molecular Structure; D014299:Trimipramine / Q000031:analogs & derivatives / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics
C070661:2-hydroxytrimipramine; D014299:Trimipramine; C040606:desmethyltrimipramine