title: Histopathological studies on the local reactions induced by complete Freund's adjuvant (CFA), bacterial lipopolysaccharide (LPS), and synthetic lipopeptide (P3C) conjugates.
abstract: The inflammatory reactions following subcutaneous application of adjuvants revealed characteristic pathological patterns. The injection of complete Freund's adjuvant (CFA) resulted in the formation of large lipid deposits encircled by an inflammatory reaction and concentrically arranged collagen bundles. Bacterial lipopolysaccharide (LPS) caused granulomatous aggregations of mononuclear cells with thrombotic vessel occlusions. Inoculation of the lipopeptide adjuvants induced accumulation of mononuclear cells with only minimal fibrotic changes which were resolved after day 28. Lipopeptide conjugates based on the head group tripalmitoyl-S-glyceryl-cysteinyl-serin (P3CS) can thus be used as effective immunogens and adjuvants without long-term tissue damage.
authors.affiliation: Institute of Immunobiology, University of Freiburg, F.R.G.
authors.forename: F
authors.initials: F
authors.lastname: Wiedemann
authors.forename: R
authors.initials: R
authors.lastname: Link
authors.forename: K
authors.initials: K
authors.lastname: Pumpe
authors.forename: U
authors.initials: U
authors.lastname: Jacobshagen
authors.forename: H E
authors.initials: HE
authors.lastname: Schaefer
authors.forename: K H
authors.initials: KH
authors.lastname: Wiesmüller
authors.forename: R P
authors.initials: RP
authors.lastname: Hummel
authors.forename: G
authors.initials: G
authors.lastname: Jung
authors.forename: W
authors.initials: W
authors.lastname: Bessler
authors.forename: T
authors.initials: T
authors.lastname: Böltz
mesh_terms: D000818:Animals; D003877:Dermatitis, Contact / Q000473:pathology*; D005260:Female; D005487:Foam Cells / Q000473:pathology; D005620:Freund's Adjuvant / Q000276:immunology*; D007074:Immunoglobulin G / Q000032:analysis; D008070:Lipopolysaccharides / Q000276:immunology*; D008074:Lipoproteins / Q000276:immunology*; D008214:Lymphocytes / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000473:pathology; D012867:Skin / Q000473:pathology; D013927:Thrombosis / Q000473:pathology; D014699:Venules / Q000473:pathology
chemical_list: D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D008074:Lipoproteins; D005620:Freund's Adjuvant