Normal unstimulated lymphocytes produce granulopoietic inhibitory activity.
Normal unstimulated lymphocytes cocultured with normal bone marrow will inhibit day-7 colony- forming units in culture. We have shown that this phenomenon has a molecular basis and attempted to characterize it further. Using specific assays and neutralizing monoclonal antibodies we have found that this granulopoietic inhibitory activity (GIA) is not due to alpha- or gamma-interferon, tumor necrosis factor, or acidic or basic isoferritins. Biochemical studies suggest that it is a glycoprotein with a molecular weight greater than 100,000 daltons. It appears to act on cells in S phase, although it may not be S-phase specific. GIA represents a novel inhibitor that merits further investigation.
Irvine
A E
AE
Department of Haematology, Queen's University of Belfast, Northern Ireland.
French
M A
MA
Bridges
J M
JM
Crockard
A D
AD
Desai
Z R
ZR
Morris
T C
TC
D001853:Bone Marrow / Q000502:physiology; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D006023:Glycoproteins / Q000378:metabolism / Q000502:physiology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000037:antagonists & inhibitors; D006098:Granulocytes / Q000502:physiology*; D006410:Hematopoiesis / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007370:Interferon Type I / Q000502:physiology; D007371:Interferon-gamma / Q000502:physiology; D008213:Lymphocyte Activation / Q000502:physiology; D008214:Lymphocytes / Q000378:metabolism / Q000502:physiology*; D016196:S Phase / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
D006023:Glycoproteins; D007370:Interferon Type I; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor