[Detection and identification of new cardioactive proteins in cattle heart].
Seven cardioactive polypeptides were identified in precardiac and auricular regions of bovine heart. Procedures used for purification of the polypeptides involved extraction of water-soluble proteins, fractionation by ammonium sulfate, gel filtration on Sephadex G-100, chromatography and rechromatography on DEAE cellulose. Some physico-chemical and biological properties were studied; amino acid composition, N-terminal amino acids, molecular mass and the effects on coronary blood vessels. The data obtained suggest that multiple forms of these proteins may exist either as a result of partial hydrolysis of large molecules or due to processing of precursors.
Popova
T V
TV
Srapionian
R M
RM
Galoian
A A
AA
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D003331:Coronary Vessels / Q000187:drug effects; D008970:Molecular Weight; D009124:Muscle Proteins / Q000302:isolation & purification* / Q000494:pharmacology / Q000502:physiology; D009206:Myocardium / Q000737:chemistry*
D000596:Amino Acids; D009124:Muscle Proteins
Obnaruzhenie i identifikatsiia v serdtse byka novykh kardioaktivnykh belkov.