Effect of 17 beta-estradiol on PAF and prostaglandin levels in oophorectomized rat uterus.
The effects of 17 beta-estradiol on the levels of platelet-activating factor (PAF) and prostaglandins and their precursor phospholipid in the uterus of oophorectomized rats were studied. Oophorectomy results in the decrease in the uterine PAF level to one-third of that in natural estrus. This level was recovered by subcutaneous administration of 17 beta-estradiol. The level of uterine phospholipids, which are rich in arachidonic acid, was significantly decreased by estradiol treatment. More arachidonate-PC was depleted than arachidonate-PE. The molecular structure was confirmed by gas chromatography-mass spectrometry. The amount of PGF2 alpha in the oophorectomized uterine tissue was 10-times that of PAF, but like the latter, increased 3-4 times on estradiol treatment. The chemical structures of PAF and PGF2 alpha formed on estradiol treatment were confirmed by mass spectrometry. The present data strongly suggest a correlation between the formations of PAF and PGF2 alpha, and indicate that estradiol may regulate the physiological formations of PAF and PGs in non-pregnant rat uterus.
Nakayama
R
R
Department of Medical Chemistry, Kansai Medical School Moriguchi, Osaka, Japan.
Yasuda
K
K
Okumura
T
T
Saito
K
K
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D004958:Estradiol / Q000502:physiology*; D005260:Female; D009929:Organ Size; D010052:Ovariectomy; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010972:Platelet Activating Factor / Q000378:metabolism*; D011453:Prostaglandins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014599:Uterus / Q000033:anatomy & histology / Q000378:metabolism* / Q000601:surgery
D001095:Arachidonic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010972:Platelet Activating Factor; D011453:Prostaglandins; D004958:Estradiol