Tissue and subcellular distributions of an inhibitory GDP/GTP exchange protein (GDI) for smg p25A by use of its antibody.
We have recently purified from bovine brain cytosol to near homogeneity a GDP/GTP exchange protein for smg p25A, named smg p25A GDI, that inhibits the dissociation of GDP from and the subsequent binding of GTP to smg p25A. In the present study, we made an antiserum against smg p25A GDI and studied its tissue distribution in rat and its subcellular distribution in rat cerebrum by use of this antiserum. smg p25A GDI was found in secretory cells with both regulated and constitutive secretion types. Since smg p25A was previously found in only secretory cells with a regulated secretion type, this result suggests that small GTP-binding proteins different from smg p25A but recognized by smg p25A GDI are present in secretory cells with a constitutive secretion type, and that smg p25A GDI is involved in both regulated and constitutive secretory processes. In subcellular fractionation analysis of rat cerebrum, smg p25A GDI was mostly found in the cytosol fraction of neuron body and synaptosome. In synaptosome, it was mainly found in the synaptic cytosol.
Nonaka
H
H
Department of Biochemistry, Kobe University School of Medicine, Japan.
Kaibuchi
K
K
Shimizu
K
K
Yamamoto
J
J
Takai
Y
Y
D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D019204:GTP-Binding Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D020730:Guanine Nucleotide Dissociation Inhibitors; D015151:Immunoblotting; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000378:metabolism; D020692:rab3 GTP-Binding Proteins; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors
D000906:Antibodies; D000936:Antigen-Antibody Complex; D020730:Guanine Nucleotide Dissociation Inhibitors; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors; D019204:GTP-Binding Proteins; D020692:rab3 GTP-Binding Proteins