Isolation and identification of alpha-(beta-alanyl)hypusine from bovine brain.
A unique dipeptide was isolated from bovine brain using five steps of ion-exchange chromatography. Its acid hydrolysate contained equimolar amounts of beta-alanine and hypusine. The structure of the peptide was elucidated as alpha-(beta-alanyl)hypusine using dansylation technique. About 1 mumol of the compound was isolated from 1090 g of bovine brain.
Ueno
S
S
Department of Neuropsychiatry, Ehime University, School of Medicine, Japan.
Kotani
K
K
Sano
A
A
Kakimoto
Y
Y
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D004151:Dipeptides / Q000302:isolation & purification*; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry; D015091:beta-Alanine / Q000737:chemistry*
D004151:Dipeptides; D015091:beta-Alanine; C067248:alpha-(beta-alanyl)hypusine; C100028:hypusine; D008239:Lysine