Alterations and reversibility of the effects of gossypol acetic acid on the intestinal uptake of end product nutrients in normal and protein calorie-malnourished male rats.
The effects of gossypol acetic acid, an experimental male antifertility agent, 20 mg/kg body weight daily for 15 days and its subsequent withdrawal have been investigated on the intestinal uptake of certain end product nutrients, namely glucose, leucine, alanine and calcium, in normal and protein-calorie-malnourished (PCM) male albino rats. Gossypol feeding caused a reduction in body weight, intestinal weight and length, protein and nucleic acid contents in both normal and PCM animals. Serum parameters, e.g. total protein, albumin and globulin, also showed a decrease in PCM rats. PCM per se considerably elevated the uptake of nutrients but gossypol feeding inhibited the uptake of nutrients both in normal-fed and malnourished animals. Gossypol caused a decrease in the Na(+)-dependent (active) glucose uptake while the Na(+)-independent (passive) process was not altered. The kinetic parameters of glucose uptake indicate that gossypol might be interfering with the transport/carrier protein of these nutrients as reduction in maximum uptake velocity (Jmax) of these systems was observed without, however, any change in the affinity constant (Kt).
Chadha
S
S
Department of Zoology, Panjab University, Chandigarh, India.
Sanyal
S N
SN
Kanwar
U
U
D000596:Amino Acids / Q000493:pharmacokinetics; D000818:Animals; D002118:Calcium / Q000493:pharmacokinetics; D003272:Contraceptive Agents, Male / Q000494:pharmacology*; D005947:Glucose / Q000493:pharmacokinetics; D006072:Gossypol / Q000031:analogs & derivatives* / Q000494:pharmacology; D007408:Intestinal Absorption / Q000187:drug effects*; D008297:Male; D009747:Nutritional Physiological Phenomena / Q000502:physiology; D011502:Protein-Energy Malnutrition / Q000378:metabolism*; D051381:Rats
D000596:Amino Acids; D003272:Contraceptive Agents, Male; D005947:Glucose; D006072:Gossypol; C028178:gossypol acetic acid; D002118:Calcium