The murine rac1 gene: cDNA cloning, tissue distribution and regulated expression of rac1 mRNA by disassembly of actin microfilaments.
A 886 bp cDNA encoding the murine rac1 protein has been isolated. The abundance of rac1 mRNA was determined in fourteen tissues from both mouse and pig. The mRNA 5' non-coding sequence is very rich in G + C, and has the potential to form several stable secondary structures. In addition, this region contains a putative open reading frame of 57 amino acids. Disruption of the actin microfilament network by cytochalasin B in LLC-PK1 cells results in down regulation of rac1 mRNA. In agreement with the proposed role of rac1 in exocytosis, these results could explain the inhibitory effect of cytochalasin B on secretory processes.
Moll
J
J
Department of Biotechnology, Ciba-Geigy Ltd., Basel, Switzerland.
Sansig
G
G
Fattori
E
E
van der Putten
H
H
D008841:Actin Cytoskeleton / Q000502:physiology* / Q000648:ultrastructure; D000199:Actins / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D019204:GTP-Binding Proteins / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D015723:Gene Library; D051379:Mice; D008969:Molecular Sequence Data; D015739:Nocodazole / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D015345:Oligonucleotide Probes; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis / Q000187:drug effects / Q000235:genetics; D013552:Swine; D020744:rac GTP-Binding Proteins
D000199:Actins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D003571:Cytochalasin B; D004247:DNA; D019204:GTP-Binding Proteins; D020744:rac GTP-Binding Proteins; D015739:Nocodazole; D003078:Colchicine