v-myb transformation of Xeroderma pigmentosum human fibroblasts: overexpression of the c-Ha-ras oncogene in the transformed cells.
Human Xeroderma pigmentosum "normal" fibroblasts AS16 (XP4 VI) were transformed after transfection with a recombinant v-myb clone. In this clone (pKXA 3457) derived from avian myeloblastosis virus (AMV), the expression of the oncogene sequences is driven by the AMV U-5 LTR promoter. The transformed cells (ASKXA), which have integrated a rearranged v-myb oncogene, grow in agar, are not tumorigenic in nude mice, and express a 45-kDa v-myb protein. The HMW DNA of these cells transform chicken embryo fibroblasts. The c-Ha-ras oncogene is overexpressed in the ASKXA cells but not in the parental "normal" AS16 cells and a revertant clone (ASKXA Cl 1.1 G). Our results lead to the conclusion that the XP fibroblasts are phenotypically transformed by the presence of the transfected v-myb oncogene, which is able to induce an overexpression of the c-Ha-ras gene.
Michelin
S
S
Laboratoire de Génétique Moléculaire, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
Varlet
I
I
Martinerie
C
C
Perbal
B
B
Sarasin
A
A
Suárez
H G
HG
D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000235:genetics; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D003001:Cloning, Molecular; D004260:DNA Repair; D004261:DNA Replication / Q000187:drug effects; D011905:Genes, ras; D006801:Humans; D009857:Oncogenes; D010641:Phenotype; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014162:Transfection; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum