title: Rapid enzymatic method for the measurement of mannitol in urine.
abstract: A previously described method for mannitol in urine has been modified and improved. End product inhibition by fructose in the mannitol dehydrogenase method for mannitol has been minimized; the assay is linear over a sample mannitol concentration range of 0-12 mmol/L; no significant interference from other sugars or sugar alcohols could be demonstrated. The method is precise (within-batch CV less than 1%), rapid and shows excellent recovery of mannitol in spiked samples. Comparison with gas liquid chromatography shows excellent correlation (r = 0.994) between the two methods.
authors.affiliation: Department of Chemical Pathology, Derbyshire Royal Infirmary, Derby, UK.
authors.forename: J
authors.initials: J
authors.lastname: Blood
authors.forename: A R
authors.initials: AR
authors.lastname: Ingle
authors.forename: N
authors.initials: N
authors.lastname: Allison
authors.forename: G R
authors.initials: GR
authors.lastname: Davies
authors.forename: P G
authors.initials: PG
authors.lastname: Hill
mesh_terms: D002849:Chromatography, Gas; D015510:Clinical Medicine / Q000379:methods*; D006593:Hexokinase / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008353:Mannitol / Q000652:urine*; D008354:Mannitol Dehydrogenases
chemical_list: D008353:Mannitol; D008354:Mannitol Dehydrogenases; D006593:Hexokinase