Flow cytometric DNA analysis of neuroblastoma: prognostic significance of DNA ploidy in unfavorable group.
Flow cytometric DNA content analyses were performed on samples of 54 patients with neuroblastoma. DNA aneuploidy was detected in 55.6% of the 54 patients. A high incidence of DNA aneuploidy was observed in patients with prognostically favorable variables such as age (less than 1 year), clinical stage (I, II, or IVs), and primary site (extraadrenal sites). DNA aneuploidy was predominant in surviving patients, even in those with unfavorable variables. In patients 1 year old or more, the survival rate among those with DNA aneuploidy was 58.8% compared with 28.6% in patients with diploidy. Likewise, in patients with advanced stage (III or IV) neuroblastoma, the survival rate among those with DNA aneuploidy was 63.2% compared with 30.4% in patients with DNA diploidy. It is concluded that DNA content analysis is of value in predicting the prognosis of patients with neuroblastoma.
Naito
M
M
Department of Pediatric Surgery, Niigata University Hospital, Japan.
Iwafuchi
M
M
Ohsawa
Y
Y
Uchiyama
M
M
Hirota
M
M
Matsuda
Y
Y
Iinuma
Y
Y
D000310:Adrenal Gland Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D000782:Aneuploidy; D002648:Child; D002675:Child, Preschool; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D004171:Diploidy; D005434:Flow Cytometry; D005729:Ganglioneuroma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D006801:Humans; D007223:Infant; D009367:Neoplasm Staging; D009447:Neuroblastoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D011379:Prognosis
D004273:DNA, Neoplasm