Extraction strategy for obtaining DNA from bloodstains for PCR amplification and typing of the HLA-DQ alpha gene.
A simple, practical approach for the extraction of PCR-amplifiable DNA for the HLA-DQ alpha gene from bloodstains deposited on various substrates is described. DNA from bloodstains is purified using Chelex 100 ion-exchange resin and then amplified. If amplification is not achieved, the extract is washed through a Centricon 100 dialysis/concentration tube. If the second amplification of this extract produces a negative result, the extract is processed with Chelex 100 again. This approach has been found to be reliable, safe, efficient and economical.
Jung
J M
JM
Forensic Science Research and Training Center, FBI Academy, Quantico, VA 22135.
Comey
C T
CT
Baer
D B
DB
Budowle
B
B
D001801:Blood Stains; D004247:DNA / Q000302:isolation & purification*; D006683:HLA-DQ Antigens / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction
D006683:HLA-DQ Antigens; D004247:DNA