title: Detection of Anaplasma marginale rickettsemia prior to onset of clinical signs by using an antigen capture enzyme-linked immunosorbent assay.
abstract: An antigen capture enzyme-linked immunosorbent assay was developed by using monoclonal antibodies to conserved epitopes on the Anaplasma marginale MSP1a surface protein. The assay sensitivity was 1.1 (+/- 0.5)% parasitized erythrocytes, and all infected cattle were detected prior to development of 2.0%-parasitized erythrocytes. Positive tests preceded the onset of anemia by a mean of 2 days. The assay was specific for anaplasmosis, as demonstrated by nonreactivity with another common hemoparasitic pathogens.
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.
authors.forename: E S
authors.initials: ES
authors.lastname: Trueblood
authors.forename: T C
authors.initials: TC
authors.lastname: McGuire
authors.forename: G H
authors.initials: GH
authors.lastname: Palmer
mesh_terms: D000712:Anaplasmosis / Q000175:diagnosis*; D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000175:diagnosis / Q000662:veterinary
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial