Reassignment of the human CSF1 gene to chromosome 1p13-p21.
Human macrophage colony-stimulating factor (CSF-1 or M-CSF) is encoded by a single gene that was previously assigned to the long arm of chromosome 5, band q33.1, in a region adjacent to the gene encoding its receptor (Pettenati MJ, et al, Proc Natl Acad Sci USA 84:2970, 1987). Using fluorescence in situ hybridization with genomic probes to examine normal metaphase chromosomes, we reassigned the human CSF1 gene to the short arm of chromosome 1, bands p13-p21. We confirmed this result by hybridizing a CSF1 cDNA probe to filters containing flow-sorted chromosomes and by identifying CSF1 sequences in DNAs extracted from human x rodent somatic cell hybrids that contained human chromosome 1 but not human chromosome 5. Our findings are consistent with studies that have shown tight linkage between the murine CSF1 and amylase genes, as part of a conserved linkage group between mouse chromosome 3 and the short arm of human chromosome 1, which also includes the genes encoding the beta subunits of thyrotropin and nerve growth factor. Assignment of the CSF1 gene to chromosome 1 at bands p13-p21 raises the possibility that it may be altered by certain nonrandom chromosomal abnormalities arising in human hematopoietic malignancies and solid tumors.
Morris
S W
SW
Department of Hematology-Oncology, St Jude Children's Research Hospital, Memphis, TN 38105.
Valentine
M B
MB
Shapiro
D N
DN
Sublett
J E
JE
Deaven
L L
LL
Foust
J T
JT
Roberts
W M
WM
Cerretti
D P
DP
Look
A T
AT
D002460:Cell Line; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1 / Q000737:chemistry*; D004247:DNA / Q000235:genetics; D005091:Exons; D005434:Flow Cytometry; D005453:Fluorescence; D006801:Humans; D006822:Hybrid Cells; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics*; D009693:Nucleic Acid Hybridization
D016173:Macrophage Colony-Stimulating Factor; D004247:DNA