Bioassay for siderophore utilization by Candida albicans.
A convenient plate assay which is sensitive to medium pH has been developed to evaluate potential siderophores of Candida albicans. Adding a siderophore to a filter paper disk on chemically defined Lee's agar (final pH 7.2) seeded with the test strain reversed the growth inhibitory effects of the supplemented (25-100 micrograms/ml) iron chelator ethylenediaminedi(o-hydroxyphenylacetic acid), to provide a zone of growth stimulation. This bioassay has been used to demonstrate the structure-activity relationships of ferrichrome and several water-soluble hydroxamate peptide building blocks of this natural siderophore. Of all compounds so evaluated, ferrichrome exhibited the best activity.
Minnick
A A
AA
Department of Chemistry and Biochemistry, University of Notre Dame, Indiana 46556.
Eizember
L E
LE
McKee
J A
JA
Dolence
E K
EK
Miller
M J
MJ
D002176:Candida albicans / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D004492:Edetic Acid / Q000494:pharmacology / Q000031:analogs & derivatives; D005291:Ferrichrome / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism*; D008670:Metals / Q000378:metabolism; D008722:Methods; D010455:Peptides / Q000378:metabolism
D008670:Metals; D010455:Peptides; D005291:Ferrichrome; C026058:EDDA; D004492:Edetic Acid; D007501:Iron