Protective effect of vitamin E on linoleic acid hydroperoxide-induced injury to human endothelial cells.
The protective effects of vitamin E (alpha-tocopherol) and its model compounds, which act as antioxidants, on linoleic acid hydroperoxide-induced injury to human umbilical vein endothelial cells were examined. When incubated at 50 microM with endothelial cells at 37 degrees C for 24 hr, alpha-tocopherol protected the cells from injury, and 2,2,5,7,8-pentamethylchroman-6-ol showed a similar protective effect. Trolox C, a water-soluble vitamin E model compound, had no protective effect. Tocol, a poor antioxidant, proved toxic. During preincubation, alpha-tocopherol was incorporated into the cells at 16.6 nmol/mg protein, while the pentamethylchromanol was incorporated at 0.5 nmol/mg protein; Trolox C was not incorporated at all. The results suggest that agents having both high antioxidant activity and lipophilicity can protect endothelial cells from linoleic acid hydroperoxide-induced injury.
Kaneko
T
T
Tokyo Metropolitan Institute of Gerontology, Japan.
Nakano
S
S
Matsuo
M
M
D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002839:Chromans / Q000378:metabolism / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008041:Linoleic Acids / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000494:pharmacology*; D014471:Umbilical Veins; D014810:Vitamin E / Q000378:metabolism / Q000494:pharmacology*
D000975:Antioxidants; D002839:Chromans; D008041:Linoleic Acids; D008054:Lipid Peroxides; D014810:Vitamin E; C008285:linoleic acid hydroperoxide; C029141:2,2,5,7,8-pentamethyl-1-hydroxychroman; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid