title: Immaturity and aneuploidy in human oocytes after different stimulation protocols.
abstract: OBJECTIVE
  To study immaturity and aneuploidy in human oocytes after two different stimulation protocols.
  
  
  METHODS
  Retrospective.
  
  
  METHODS
  Outpatient IVF clinic/laboratory.
  
  
  METHODS
  One hundred forty-three patients of whom 65 were stimulated with clomiphene citrate (CC)/human menopausal gonadotropin (hMG) and 78 were stimulated with gonadotropin-releasing hormone agonist (GnRH-a)/hMG. Only patients with at least one oocyte unfertilized were included in this study.
  
  
  RESULTS
  Stimulation with GnRH-a/hMG, as compared with CC/hMG stimulation, resulted in larger numbers of oocytes (P less than 0.00001), a higher fertilization rate (P less than 0.02), and oocyte retrieval at a later average cycle day (P less than 0.000005). Cytogenetic findings of immaturity were observed in 33.9% of unfertilized oocytes after CC/hMG stimulation, compared with only 17.8% after GnRH-a/hMG stimulation (P less than 0.0005). Aneuploidy findings were the same for both groups.
  
  
  CONCLUSIONS
  In GnRH-a/hMG stimulation, oocytes approach the normal day of ovulation more closely. This may allow for better oocyte maturation and higher fertilization and pregnancy rates.
authors.affiliation: Academische Ziekenhuis Maastricht, The Netherlands.
authors.forename: M H
authors.initials: MH
authors.lastname: Pieters
authors.forename: J C
authors.initials: JC
authors.lastname: Dumoulin
authors.forename: C M
authors.initials: CM
authors.lastname: Engelhart
authors.forename: M
authors.initials: M
authors.lastname: Bras
authors.forename: J L
authors.initials: JL
authors.lastname: Evers
authors.forename: J P
authors.initials: JP
authors.lastname: Geraedts
mesh_terms: D000782:Aneuploidy; D002064:Buserelin / Q000008:administration & dosage; D002985:Clinical Protocols; D002996:Clomiphene / Q000008:administration & dosage; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008596:Menotropins / Q000008:administration & dosage; D008597:Menstrual Cycle; D009865:Oocytes / Q000166:cytology* / Q000502:physiology; D010062:Ovulation Induction / Q000379:methods*; D011247:Pregnancy; D012189:Retrospective Studies
chemical_list: D002996:Clomiphene; D008596:Menotropins; D002064:Buserelin