title: The primary structure of the hemoglobin from the aardwolf (Proteles cristatus, Hyaenidae).
abstract: The hemoglobin of the aardwolf (Proteles cristatus) contains only one component. In this paper, we are presenting its primary structure. The globin chains were separated by high-performance liquid chromatography and the sequences determined by automatic liquid and gas-phase Edman degradation of the chains and their tryptic peptides. The alpha- as well as the beta-chains show 20 exchanges compared with the corresponding human chains. The difference to the masked palm civet (Paguma larvata) and the spotted hyaena (Crocuta crocuta) is marked by 16 and 4 replacements in the alpha-chains and by 10 and 1 in the beta-chains, thus supporting the hyaenid character of the aardwolf. The exchanges at contact positions are shared by other carnivoran hemoglobins.
authors.affiliation: Max-Planck-Institut für Biochemie, Abteilung Proteinchemie, Martinsried bei Munchen.
authors.forename: S
authors.initials: S
authors.lastname: Stoeva
authors.forename: T
authors.initials: T
authors.lastname: Kleinschmidt
authors.forename: G
authors.initials: G
authors.lastname: Braunitzer
authors.forename: H G
authors.initials: HG
authors.lastname: Scheil
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002335:Carnivora / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D000882:Haplorhini / Q000235:genetics; D006441:Hemoglobin A / Q000235:genetics; D006454:Hemoglobins / Q000235:genetics*; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid
chemical_list: D006454:Hemoglobins; D046911:Macromolecular Substances; D010446:Peptide Fragments; D006441:Hemoglobin A