Mitogenicity and metallothionein induction: two separate effects of zinc ions on human mononuclear blood cells.
The mitogenic effect of elevated concentrations of zinc ions on human lymphocytes was found to be inhibited by the glucocorticoid hormone dexamethasone. The effect decreased progressively from complete block to partial inhibition when the culture period before the addition of dexamethasone was prolonged. In contrast, dexamethasone did not inhibit an induced formation of metallothionein in mononuclear cells. Furthermore, dexamethasone itself was found to induce small amounts of metallothionein. Apparently, the mitogenic effect and the induction of metallothionein by zinc ions in mononuclear cells occur by mutually independent mechanisms.
Steffensen
I L
IL
Department of Environmental Medicine, National Institute of Public Health, Oslo, Norway.
Mesna
O J
OJ
Melhuus
A
A
Hjertholm
H
H
Heier
H E
HE
Andersen
R A
RA
D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008668:Metallothionein / Q000096:biosynthesis* / Q000302:isolation & purification; D008934:Mitogens / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D015032:Zinc / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D008934:Mitogens; D003907:Dexamethasone; D008668:Metallothionein; D015032:Zinc