Preparation of iodine labeled recombinant human erythropoietin.
125I-labelled recombinant human erythropoietin (125I-rh-EPO; CAS for EPO: 11096-26-7) was prepared by using iodo-gen (1,3,4,6-tetrachloro-3,6-diphenylglycouril), which showed a similar immunoreactivity to anti rh-EPO anti-rabbit serum and a similar pharmacokinetics with that of non-labeled rh-EPO.
Kinoshita
H
H
Drug Metabolism Laboratory, Chugai Pharmaceutical Co., Ltd., Tokyo, Japan.
Ohishi
N
N
Okazaki
A
A
D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004921:Erythropoietin / Q000096:biosynthesis* / Q000276:immunology / Q000493:pharmacokinetics; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D051379:Mice; D011086:Polycythemia / Q000188:drug therapy; D011233:Precipitin Tests; D011863:Radioimmunoassay; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000493:pharmacokinetics
D007457:Iodine Radioisotopes; D011994:Recombinant Proteins; D004921:Erythropoietin