title: Interferon-gamma inhibition suppresses experimental allergic neuritis: modulation of major histocompatibility complex expression of Schwann cells in vitro.
abstract: This study examines the modulation of major histocompatibility complex (MHC) expression on Lewis rat Schwann cells (Scs) cultured in the presence of dorsal root ganglion neurons (DRG). MHC class I and II molecules were induced on Scs using recombinant murine interferon-gamma (IFN-gamma), lymph node cells (LNC), removed at day 9 from Lewis rats with experimental allergic neuritis (EAN) and syngeneic T-cell line cells responsive to P2 basic protein. EAN LNC induced MHC class I on Scs but only IFN-gamma or P2-responsive T-cells induced MHC class II. Control LNC from animals injected with Freund's adjuvant alone or naive spleen cells did not induce MHC class II. P2 T-cells clustered in aggregates to the Scs. Similar studies were performed with inhibitors of IFN-gamma; hydrocortisone, cyclosporin A, dibutyryl cyclic AMP, methyl-xanthine and prostaglandin E2. Each agent produced a dose-dependent inhibition of MHC expression and prevented clustering of P2-responsive T-cells to Scs.
authors.affiliation: Department of Medicine, University of Sydney, N.S.W., Australia.
authors.forename: C P
authors.initials: CP
authors.lastname: Tsai
authors.forename: J D
authors.initials: JD
authors.lastname: Pollard
authors.forename: P J
authors.initials: PJ
authors.lastname: Armati
mesh_terms: D000818:Animals; D002449:Cell Aggregation; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007371:Interferon-gamma / Q000037:antagonists & inhibitors*; D008285:Major Histocompatibility Complex / Q000276:immunology*; D009444:Neuritis, Autoimmune, Experimental / Q000503:physiopathology*; D012583:Schwann Cells / Q000276:immunology*; D013601:T-Lymphocytes / Q000502:physiology
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007166:Immunosuppressive Agents; D007371:Interferon-gamma