title: Protective immunity to Schistosoma mansoni in mice is dependent on antibody and complement but not on radiosensitive leukocytes.
abstract: The role of complement in the control of the Schistosoma mansoni infection in mice was investigated in vivo. The number of schistosomula recovered from the lung 5 days post-infection was used as a parasitological criterion of immunity. A significant difference in worm burden was observed between normal and immune mice. In contrast, when cobra venom factor (CVF) was injected into normal or immune mice 3 h before challenge, a significant increase in worm burden was noticed compared to untreated mice. We also investigated the protective mechanisms in mice that had been exposed to 650 rads of 60Co gamma radiation before challenge infection. Our results show that gamma-irradiated immune mice, depleted of more than 90% of their circulating or tissue leukocytes, are still able to destroy most of the parasites of a challenge infection with cercariae, suggesting that the radiosensitive leukocytes are not essential in the effector mechanisms of this protective immunity to S. mansoni. These results provide evidence of a role for the complement system, in association with radioresistant effector cells, in protective immunity occurring in the first hours after infection with S. mansoni.
authors.affiliation: Departamento de Bioquimica-Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horzonte, Brazil.
authors.forename: A M
authors.initials: AM
authors.lastname: Goes
authors.forename: F J
authors.initials: FJ
authors.lastname: Ramalho-Pinto
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth; D003165:Complement System Proteins; D004546:Elapid Venoms / Q000494:pharmacology; D007107:Immune System / Q000528:radiation effects; D007116:Immunization, Passive; D007962:Leukocytes / Q000276:immunology / Q000528:radiation effects; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011836:Radiation Tolerance; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology* / Q000517:prevention & control
chemical_list: D000909:Antibodies, Helminth; D004546:Elapid Venoms; C016310:cobra venom factor; D003165:Complement System Proteins