Schistosoma mansoni tropomyosin: production and purification of the recombinant protein and studies on its immunodiagnostic potential.
A cDNA that encodes Schistosoma mansoni tropomyosin, except for 10 amino acids at the amino terminus, has been cloned into a pOTSNCO plasmid vector. Induced expression resulted in a constant level of recombinant protein production. The recombinant S. mansoni tropomyosin was purified from preparative SDS-PAGE gel and by a combination of 20% ammonium sulfate fractionation and fast protein liquid chromatography-ion-exchange chromatography. The purified recombinant S. mansoni tropomyosin was tested as an immunodiagnostic reagent in Western blot and enzyme-linked immunosorbent assays. Sera from individual patients with chronic S. mansoni infection, but not S. haematobium, S. japonicum, parasitic infections other than schistosomiasis, and without infection reacted with the recombinant tropomyosin. The species specificity of S. mansoni tropomyosin suggests that further study of its potential as an immunodiagnostic reagent is warranted.
Xu
H
H
Department of Microbiology, School of Medicine, State University of New York, Buffalo.
Rekosh
D M
DM
Andrews
W
W
Higashi
G I
GI
Nicholson
L
L
Loverde
P T
PT
D000818:Animals; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D012550:Schistosoma mansoni / Q000276:immunology / Q000378:metabolism*; D012555:Schistosomiasis mansoni / Q000175:diagnosis* / Q000276:immunology; D014335:Tropomyosin / Q000096:biosynthesis / Q000302:isolation & purification*
D011994:Recombinant Proteins; D014335:Tropomyosin