Heterologous in vitro transcription from two archaebacterial promoters.
A cell-free extract of Sulfolobus shibatae is able to specifically initiate transcription in vitro at the promoter of the plasmid-encoded gene for the major gas vesicle protein of Halobacterium halobium and at the promoter for the transcript T4 of the temperate H. halobium phage phi H. The corresponding promoter from the virulent phage mutant phi HL1 yields enhanced transcription in the heterologous system, in agreement with strongly increased in vivo expression.
Hüdepohl
U
U
Max-Planck Institut für Biochemie, München, Germany.
Gropp
F
F
Horne
M
M
Zillig
W
W
D001105:Archaea / Q000235:genetics*; D019843:Archaeal Proteins; D001426:Bacterial Proteins / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D005075:Biological Evolution; D002474:Cell-Free System / Q000378:metabolism; D006217:Halobacterium / Q000235:genetics; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011506:Proteins; D015183:Restriction Mapping; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*
D019843:Archaeal Proteins; D001426:Bacterial Proteins; D008565:Membrane Proteins; D011506:Proteins; D014157:Transcription Factors; C038524:gas vesicle protein; D015719:Single-Strand Specific DNA and RNA Endonucleases