Effect of thrombin on the production of glycosaminoglycans by cultured endothelial cells.
We investigated the effect of thrombin on the production process of glycosaminoglycans (GAG) in cultured bovine aortic endothelial cells. It was revealed that the trichloroacetic acid-insoluble [35S]sulfate-labeled GAG (35S-GAG) was decreased by thrombin. The thrombin-induced decrease in the accumulation of 35S-GAG in the cell layer occurred even in the presence of actinomycin D or cycloheximide. The incorporation of both [35S]sulfate and [3H]glucosamine into the GAG was decreased by thrombin, while the ratio of 35S to 3H was not changed. Also, the incorporation of both [3H]glucosamine and [14C]UDP-xylose into the GAG was inhibited by thrombin. From these results, it was suggested that thrombin decreased GAG in the endothelial cell layer through an suppression of formation of polysaccharide chains rather than that of core protein.
Kaji
T
T
Department of Environmental Science, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
Akai
T
T
Hayashi
T
T
Sakuragawa
N
N
D000818:Animals; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D011134:Polysaccharides / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology*; D014540:Uridine Diphosphate Xylose / Q000378:metabolism
D006025:Glycosaminoglycans; D011134:Polysaccharides; D011506:Proteins; D013431:Sulfates; D003609:Dactinomycin; D014540:Uridine Diphosphate Xylose; D003513:Cycloheximide; D013917:Thrombin; D005944:Glucosamine