Spectrophotometrical and immunochemical studies on the conformational changes in poly(dG-dC).poly(dG-dC) after modification by 4-hydroxyaminoquinoline 1-oxide.
Poly(dG-dC).poly(dG-dC) was modified by the reaction with 4-hydroxyaminoquinoline 1-oxide (4HAQO) in the presence of seryl-AMP. The conformations of 4HAQO-modified poly(dG-dC).poly(dG-dC) and of poly(dG-dC).poly(dG-dC) were studied by circular dichroism spectra under various salt concentration conditions. 4HAQO residues to guanine bases are inefficient in inducing the transition of poly(dG-dC).poly(dG-dC) from B-form to Z-form conformation. We have elicited monoclonal antibodies against 4HAQO-poly(dG-dC).poly(dG-dC). They were characterized using enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA) and binding to supercoiled DNA. These antibodies reacted with 4HAQO-poly(dG-dC).poly(dG-dC) specifically but not with 4HAQO-modified DNA or poly(dG).poly(dC). However, they cross-reacted with N-acetoxy-2-acetylaminofluorene-modified poly(dG-dC).poly(dG-dC) in Z-form conformation. These monoclonal antibodies may recognize a unique conformation in poly(dG-dC).poly(dG-dC) after 4HAQO modification.
Tada
M
M
Laboratory of Biochemistry, Aichi Cancer Center, Nagoya, Japan.
Ikeda
S
S
Suzuki
M
M
Minoura
Y
Y
Kojima
M
M
Morita
T
T
D015108:4-Hydroxyaminoquinoline-1-oxide / Q000737:chemistry*; D000099:Acetoxyacetylaminofluorene / Q000737:chemistry; D000249:Adenosine Monophosphate / Q000737:chemistry; D000911:Antibodies, Monoclonal; D002942:Circular Dichroism; D004278:DNA, Superhelical / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D009690:Nucleic Acid Conformation; D011066:Poly C / Q000737:chemistry; D011068:Poly G / Q000737:chemistry; D011089:Polydeoxyribonucleotides / Q000737:chemistry* / Q000276:immunology; D011863:Radioimmunoassay; D012680:Sensitivity and Specificity; D012694:Serine / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D000911:Antibodies, Monoclonal; D004278:DNA, Superhelical; D011089:Polydeoxyribonucleotides; D011068:Poly G; C023826:poly(dC); C026628:poly(dG); C019530:poly(dC-dG); D011066:Poly C; D000249:Adenosine Monophosphate; D012694:Serine; D015108:4-Hydroxyaminoquinoline-1-oxide; D000099:Acetoxyacetylaminofluorene