title: A 60-kDa phosphorylated protein from fetal human bone.
abstract: A phosphorylated protein was isolated and purified from fetal human bone. Fetal and adult human bones were decalcified with EDTA, and the extract from the fetal bone was fractionated using Q-Sepharose anion exchange chromatography. The fraction containing Ser(P) was purified by Sephacryl S-200 molecular sieving and C4 reverse-phase HPLC. The purified protein had a molecular weight of 60000 on SDS-PAGE, where the protein was stained with Rhodamine-B. The amino acid composition of this protein was different from any other reported phosphorylated proteins in human bone. However, this phosphorylated protein was difficult to detect in the adult bone extract on SDS-PAGE.
authors.affiliation: Department of Orthopedic Surgery, Hamamatsu University School of Medicine, Shizuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: A
authors.initials: A
authors.lastname: Uchiyama
authors.forename: K
authors.initials: K
authors.lastname: Kushida
authors.forename: K
authors.initials: K
authors.lastname: Horiuchi
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Inoue
mesh_terms: D000328:Adult; D000596:Amino Acids / Q000032:analysis; D001842:Bone and Bones / Q000737:chemistry*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D005333:Fetus; D006801:Humans; D008970:Molecular Weight; D010750:Phosphoproteins / Q000302:isolation & purification*
chemical_list: D000596:Amino Acids; D010750:Phosphoproteins; D004492:Edetic Acid