Identification of a 200-kDa glycoprotein antigen of Saccharomyces cerevisiae.
SDS-PAGE analysis of the soluble proteins of the yeast Saccharomyces cerevisiae revealed a single predominant moiety of apparent size 200 kDa which was strongly periodic acid Schiff-positive. In immunoblotting experiments, human sera containing anti-S. cerevisiae antibodies were reactive only with this glycoprotein (gp200); this was demonstrated using sera containing IgG and IgA isotype-specific anti-S. cerevisiae antibodies. The antigenicity of gp200 was substantially reduced by periodate treatment. These results show that gp200 is a major glycoprotein antigen of S. cerevisiae which is immunogenic in man.
Heelan
B T
BT
Department of Immunology, University of Liverpool, U.K.
Allan
S
S
Barnes
R M
RM
D000908:Antibodies, Fungal / Q000276:immunology; D000946:Antigens, Fungal / Q000302:isolation & purification*; D003424:Crohn Disease / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification*; D006801:Humans; D015151:Immunoblotting; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000276:immunology*
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D006023:Glycoproteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G