Trypsin-resistant gp120 receptors are upregulated on short-term cultured human epidermal Langerhans cells.
The CD4 molecule is known to be the preferential receptor for the HIV1 envelope glycoprotein. Epidermal Langerhans cells (LC) are dendritic cells which express several surface antigens, among them the CD4 antigens. LC infection was suggested when these cells were seen to present buddings coincident with membrane thickening of roughly 100 nm in size. These buddings were similar in ultrastructural aspect to HIV buddings on in vitro infected promonocytic cells (U937). To clarify the exact role of CD4 molecules in LC infection induced by HIV1, we investigated the possible involvement of between native and recombinant HIV1 gp120 and the LC surface. We also assessed the expression of CD4 molecules on LC membranes dissociated by means of trypsin from their neighbouring keratinocytes. The cellular phenotype was monitored using flow cytometry. We show that human LC can bind the viral envelope protein and that this binding does not depend on CD4 protein expression. The amount of surface bound gp120 was not consistent with the amount of CD4 antigens present on LC membranes. The gp120-binding sites on LC in suspension appear to be typsin-resistant while the CD4 antigens (at least the epitopes known to bind HIV1) are trypsin-sensitive. A burst of gp120 receptor expression was detected on 1-day cultured LC while the CD4 antigens disappeared. These findings lead to the logical conclusion that the binding of gp120 is due to the presence of a LC surface molecule which is different from CD4 antigens.
Dezutter-Dambuyant
C
C
INSERM U209, Laboratoire de Recherche Dermatologique et Immunologie, Hôpital Edouard Herriot, Lyon, France.
Schmitt
D A
DA
Dusserre
N
N
Hanau
D
D
Kolbe
H V
HV
Kieny
M P
MP
Gazzolo
L
L
Macé
K
K
Pasquali
J L
JL
Olivier
R
R
D000911:Antibodies, Monoclonal / Q000276:immunology; D015704:CD4 Antigens / Q000378:metabolism; D002460:Cell Line; D004817:Epidermis / Q000382:microbiology; D015699:HIV Envelope Protein gp120 / Q000276:immunology / Q000378:metabolism*; D006679:HIV Seropositivity / Q000378:metabolism / Q000382:microbiology; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D007801:Langerhans Cells / Q000382:microbiology* / Q000648:ultrastructure; D015728:Receptors, HIV / Q000378:metabolism*; D013997:Time Factors; D014357:Trypsin / Q000378:metabolism; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D015728:Receptors, HIV; D014357:Trypsin