DNA damage-inducible loci in Salmonella typhimurium.
lac operon fusions to DNA damage-inducible (din) loci were generated in Salmonella typhimurium LT2. Many of these din fusions were efficiently repressed by cloned Escherichia coli LexA, while others were not; all required RecA for induction. Several din fusions exhibited strong inducibility and will be useful in developing an SOS induction assay in S. typhimurium to detect genotoxins.
Smith
C M
CM
Department of Cancer Biology, Harvard University School of Public Health, Boston, Massachusetts 02115.
Arany
Z
Z
Orrego
C
C
Eisenstadt
E
E
D000477:Alkylating Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000494:pharmacology; D003001:Cloning, Molecular; D004247:DNA / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D005798:Genes, Bacterial; D007763:Lac Operon / Q000502:physiology; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D012097:Repressor Proteins / Q000494:pharmacology; D013014:SOS Response, Genetics / Q000502:physiology*; D012486:Salmonella typhimurium / Q000235:genetics*; D012697:Serine Endopeptidases; D014466:Ultraviolet Rays / Q000009:adverse effects; D001616:beta-Galactosidase / Q000096:biosynthesis; D000099160:LexA Repressor Protein
D000477:Alkylating Agents; D001426:Bacterial Proteins; D004247:DNA; D016685:Mitomycin; D008937:Mitomycins; D012097:Repressor Proteins; D012697:Serine Endopeptidases; D001616:beta-Galactosidase; D000099160:LexA Repressor Protein