Normal c-abl gene protein--a nuclear component.
The subcellular distribution of the c-abl and bcr-abl gene products from KG1A and K562 cells has been studied by two different techniques. Firstly, physical disruption followed by subcellular fractionation was used to demonstrate that normal c-abl (p145) was recovered from the cytosol and the nuclear fractions of KG1A cells. In contrast, bcr-abl products were recovered exclusively from the cytosol fraction of K562 cells. Secondly, indirect immunofluorescence was used to localize c-abl protein to the cytoplasm, nuclear membrane and infrequently to the nucleus of KG1A cells and bcr-abl protein to only the cytoplasm of K562 cells. Thus both the approaches indicate that there is a component of normal c-abl products which appears to be nuclear and this is not reflected in the distribution of the bcr-abl 210 kDa protein, which remains cytosolic.
Dhut
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Medical Oncology Laboratory, Imperial Cancer Research Fund, St Bartholomew's Hospital, London, UK.
Chaplin
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T
Young
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BD
D000818:Animals; D002458:Cell Fractionation; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry* / Q000378:metabolism; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D016044:Fusion Proteins, bcr-abl / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D011233:Precipitin Tests; D016315:Proto-Oncogene Proteins c-abl / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
D016044:Fusion Proteins, bcr-abl; D016315:Proto-Oncogene Proteins c-abl