UVA-induced lipid peroxidation in cultured human fibroblasts.
The UVA irradiation of cultured human fibroblasts leads to the formation and to the release of thiobarbituric acid-reactive substances in the supernatant. The major thiobarbituric acid-reactive substance is identified by fluorescence spectroscopy and HPLC, as malondialdehyde or malondialdehyde-forming substances under the thiobarbituric acid assay conditions. Malondialdehyde formation strongly suggests a UVA-induced lipid peroxidation. Lipid peroxidation is also supported by the inhibitory effect of D,L-alpha-tocopherol, the well-known chain breaking antioxidant, by the additional malondialdehyde formation in the dark after the photooxidative stress and by membrane damage revealed by lactate dehydrogenase leakage.
Morlière
P
P
Laboratoire de Dermatologie, INSERM U. 312, Hôpital Henri Mondor, Créteil, France.
Moysan
A
A
Santus
R
R
Hüppe
G
G
Mazière
J C
JC
Dubertret
L
L
D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005347:Fibroblasts / Q000201:enzymology / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000528:radiation effects*; D008315:Malondialdehyde / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013858:Thiobarbiturates / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays
D013858:Thiobarbiturates; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; C029684:thiobarbituric acid