title: Preservation techniques for heart transplantation: comparison of hypothermic storage and hypothermic perfusion.
abstract: Preservation of the donor heart is an important and controversial subject in heart transplantation. This study compares simple hypothermic storage and hypothermic perfusion in a swine model of heart transplantation (n = 14). The donor hearts of group A (n = 7) were placed in simple hypothermic storage for 5 hours. The donor hearts of group B (n = 7) were placed onto a perfusion apparatus for 5 hours, with pressure maintained at 28 cm of H2O and a myocardial temperature of 8 to 10 degrees C. In both groups the hearts were initially protected with isosmolar potassium cardioplegic solution. The perfusate in group B contained moderate sodium, mannitol, glucose, insulin, and oxygen. The ischemic interval within both groups was 6 hours including orthotopic transplantation. Investigation was conducted at three time periods: prepreservation, postpreservation, and immediately after loading. For both groups there was nonsignificant depression of myocardial function (cardiac index, stroke index, stroke work index, ejection fraction, and wall stress) at the postpreservation period. After volume loading, for the hypothermic perfusion group there was significant improvement of myocardial function (cardiac index, p less than 0.01; stroke index, p less than 0.01) with no significant change in heart rate, systemic vascular resistance, and systolic blood pressure. There was also significant improvement in myocardial performance (p less than 0.05) for the hypothermic perfusion group after volume loading. Ultrastructural changes were minimal for both groups, and there were no major heart transplantation after 6 hours of ischemia; however, hearts retain their contractile capacity better after hypothermic perfusion than after simple hypothermic storage.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Surgery, University of British Columbia, Vancouver, Canada.
authors.forename: A K
authors.initials: AK
authors.lastname: Qayumi
authors.forename: W R
authors.initials: WR
authors.lastname: Jamieson
authors.forename: L J
authors.initials: LJ
authors.lastname: Rosado
authors.forename: C W
authors.initials: CW
authors.lastname: Tomlinson
authors.forename: M
authors.initials: M
authors.lastname: Schulzer
authors.forename: B
authors.initials: B
authors.lastname: McConville
authors.forename: K
authors.initials: K
authors.lastname: Gillespie
authors.forename: A
authors.initials: A
authors.lastname: Wong
mesh_terms: D000818:Animals; D002314:Cardioplegic Solutions; D003080:Cold Temperature; D004452:Echocardiography; D016027:Heart Transplantation / Q000502:physiology; D008854:Microscopy, Electron; D009206:Myocardium / Q000737:chemistry / Q000648:ultrastructure; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion; D013552:Swine; D013997:Time Factors
chemical_list: D002314:Cardioplegic Solutions