title: Functional role of mucoid exopolysaccharide (alginate) in antibiotic-induced and polymorphonuclear leukocyte-mediated killing of Pseudomonas aeruginosa.
abstract: We evaluated in vitro the functional role of mucoid exopolysaccharide (MEP) of Pseudomonas aeruginosa in blocking antibiotic-induced and polymorphonuclear leukocyte (PMN)-mediated pseudomonal killing. The serum-resistant P. aeruginosa isolates used were mucoid strain 144MR and its nonmucoid revertant, strain 144NM. By timed kill curves, early bacterial effects of amikacin against mucoid strain 144MR were substantially less than those observed with nonmucoid strain 144NM; this effect was reversible with enzymatic hydrolysis of MEP of strain 144MR by alginase. Also, early tobramycin uptake (15 to 30 min) by mucoid 144MR cells was less than that seen with nonmucoid strain 144NM; pretreatment of 144MR cells with alginase substantially enhanced early tobramycin uptake compared with untreated 144MR cells (P = 0.08). In strain 144NM (but not in strain 114MR) there was a notable postantibiotic leukocidal enhancement effect manifested by increased nonopsonic killing following brief exposure of these cells to supra-MIC amikacin; pretreatment of strain 144MR with alginase rendered these cells more susceptible to amikacin-induced postantibiotic leukocidal enhancement. Similarly, direct PMN-mediated nonopsonic killing of mucoid strain 144MR was significantly less than that observed with strain 144NM (P less than 0.05); pretreatment of 144MR cells with alginase rendered this strain equal to strain 144NM in susceptibility to nonopsonic killing. In addition, exogenous sodium alginate or extracted MEP of strain 144MR interfered with effective nonopsonic killing of strain 144NM by PMNs. Studies also indicated that mucoid strain 144MR was phagocytosed significantly less well than its nonmucoid mate (P less than 0.00001), an effect reversed by pretreatment of the mucoid cells with alginase. These data confirm that P. aeruginosa MEPs functionally decrease the uptake and early bactericidal effect of aminoglycosides in vitro and interfere with effective PMN-mediated nonopsonic phagocytosis and killing of mucoid strains.
authors.affiliation: Department of Medicine, Harbor-UCLA Medical Center, Torrance 90509.
authors.forename: A S
authors.initials: AS
authors.lastname: Bayer
authors.forename: D P
authors.initials: DP
authors.lastname: Speert
authors.forename: S
authors.initials: S
authors.lastname: Park
authors.forename: J
authors.initials: J
authors.lastname: Tu
authors.forename: M
authors.initials: M
authors.lastname: Witt
authors.forename: C C
authors.initials: CC
authors.lastname: Nast
authors.forename: D C
authors.initials: DC
authors.lastname: Norman
mesh_terms: D000464:Alginates; D000583:Amikacin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001770:Blood Bactericidal Activity; D006026:Glycoside Hydrolases / Q000494:pharmacology; D006801:Humans; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis; D011133:Polysaccharide-Lyases; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000502:physiology*; D014031:Tobramycin / Q000493:pharmacokinetics
chemical_list: D000464:Alginates; D000900:Anti-Bacterial Agents; D000583:Amikacin; D006026:Glycoside Hydrolases; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase; D014031:Tobramycin