Isolation of a full-length cDNA encoding mouse aromatase P450.
A full-length cDNA clone for aromatase P450 has been isolated from a pregnant mouse ovarian cDNA library. The insert of this clone (2394 bp) contains a 1509-bp open reading frame encoding 503 amino acid residues together with a 46-bp 5'-untranslated stretch and an 839-bp 3'-untranslated region to which a poly(A) tract is attached. Northern blot analysis of ovarian RNA from pregnant mice reveals a major mRNA band of 2.5 kb with a minor band of 2.1 kb. Comparison of mouse aromatase P450 with that of rat, human, and chicken shows 91, 81, and 69% identity in the nucleotide sequence and 92, 79, and 69% identity in the deduced amino acid sequence, respectively. The membrane-spanning domain of mouse aromatase P450 is estimated to be an extremely hydrophobic segment located within the N-terminal region of the molecule. Furthermore, a highly conserved heme-binding domain is noticed.
Terashima
M
M
Department of Medical Chemistry, Kochi Medical School, Japan.
Toda
K
K
Kawamoto
T
T
Kuribayashi
I
I
Ogawa
Y
Y
Maeda
T
T
Shizuta
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001141:Aromatase / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002645:Chickens; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010053:Ovary / Q000737:chemistry; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D012333:RNA, Messenger; D004247:DNA; D001141:Aromatase