title: The sheaths of Brugia microfilariae: isolation and composition.
abstract: Burgia malayi and B. pahangi microfilariae were isolated from the blood of infected Mastomys natalensis, and were exsheathed by freezing, thawing and agitation. Pure sheaths were obtained by a filtration procedure. The sheaths were found to contain about 95 mol% of amino acids, with proline, glutamic acid/glutamine, alanine, cysteine/cystine and glycine being the major components, and 5 mol% of carbohydrates, notably (N-acetyl)galactosamine, but no (N-acetyl)glucosamine.
authors.affiliation: Biochemisches Institut am Klinikum, Giessen, Federal Republic of Germany.
authors.forename: T
authors.initials: T
authors.lastname: Klonisch
authors.forename: G
authors.initials: G
authors.lastname: Bardehle
authors.forename: D
authors.initials: D
authors.lastname: Linder
authors.forename: B
authors.initials: B
authors.lastname: Boschek
authors.forename: H H
authors.initials: HH
authors.lastname: Schott
authors.forename: H
authors.initials: H
authors.lastname: Zahner
authors.forename: S
authors.initials: S
authors.lastname: Stirm
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000606:Amino Sugars / Q000032:analysis; D000818:Animals; D002009:Brugia / Q000032:analysis / Q000033:anatomy & histology*; D002499:Centrifugation, Density Gradient; D005374:Filtration; D005615:Freezing; D008297:Male; D008842:Microfilariae / Q000032:analysis / Q000033:anatomy & histology; D009115:Muridae
chemical_list: D000596:Amino Acids; D000606:Amino Sugars