Double wavelength measurement of 3,3',5,5'-tetramethylbenzidine (TMB) provides a three-fold enhancement of the ELISA measuring range.
A rapid, simple method is described which permits a three-fold enhancement of the working range of ELISA procedures using TMB as a substrate. This consists of measuring absorbance values at a wavelength away from the absorption maximum of TMB and using a predetermined multiplication factor.
Madersbacher
S
S
Immunoendocrinology Research Unit, Austrian Academy of Sciences, Innsbruck.
Berger
P
P
D001560:Benzidines; D006063:Chorionic Gonadotropin / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005640:Follicle Stimulating Hormone / Q000032:analysis; D006801:Humans
D001560:Benzidines; D006063:Chorionic Gonadotropin; C021758:3,3',5,5'-tetramethylbenzidine; D005640:Follicle Stimulating Hormone