title: Bombesin stimulates a high affinity GTPase activity in membranes of Swiss 3T3 fibroblasts.
abstract: Peptides of the bombesin family are mitogenic for Swiss 3T3 fibroblasts and in these cells stimulate the turnover of polyphosphoinositides. Recent studies have suggested that G protein(s) may be involved in the signal transduction pathway triggered by bombesin. In this study we have found and characterized a high affinity GTPase activity stimulated by bombesin in membranes of Swiss 3T3 fibroblasts. Our results support the involvement of a G protein in the response of Swiss 3T3 cells to bombesin.
authors.affiliation: Dipartimento di Fisiologia e Biochimica Generali, Università di Milano, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Morello
authors.forename: E
authors.initials: E
authors.lastname: Sturani
authors.forename: M G
authors.initials: MG
authors.lastname: Cattaneo
authors.forename: R
authors.initials: R
authors.lastname: Brambilla
authors.forename: A
authors.initials: A
authors.lastname: Vicario
authors.forename: L
authors.initials: L
authors.lastname: Alberghina
authors.forename: R
authors.initials: R
authors.lastname: Zippel
mesh_terms: D000818:Animals; D001839:Bombesin / Q000494:pharmacology*; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology*; D005347:Fibroblasts; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism; D019886:Gastrin-Releasing Peptide; D006160:Guanosine Triphosphate / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D051379:Mice; D010455:Peptides / Q000494:pharmacology; D010710:Phosphates / Q000032:analysis; D015398:Signal Transduction
chemical_list: D007295:Inositol Phosphates; D010455:Peptides; D010710:Phosphates; D019886:Gastrin-Releasing Peptide; D006160:Guanosine Triphosphate; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D008274:Magnesium; D001839:Bombesin