title: Identification and quantification of lipids from rabbit Harderian glands by gas chromatography/mass spectrometry.
abstract: Lipids from hydrolysed extracts of Harderian glands from the rabbit were examined as trimethylsilyl, acetonide, nicotinate and picolinyl esters and shown to consist mainly of acylated glycerol ethers and acylated hydroxy-glycerol ethers. Constituents amounting to 98.8% of the recovered secretion were identified. Fatty acids were mainly normal, saturated compounds with chain lengths from 12 to 24 carbon atoms; n-16:0 and n-18:0 accounted for about 40% of the identified acids. Small concentrations of iso-17:0 and unsaturated acids with 18-carbon chains were also identified. Fatty alcohols were again mainly normal-unsaturated compounds; the chains varied from C18 to C25 with 20:0-OH and 22:0-OH being the most abundant. Three types of 1-alkyl glycerols were found. The major constituents had normal, saturated chains with from 14 to 23 carbon atoms with the even carbon chains predominating. These were accompanied by hydroxylated derivatives of the 16- and 18-carbon glycerol ethers with hydroxy groups in the 10, 11 and 12 positions. Branched-chain glycerol ethers were of low concentration and contained predominantly iso chains. Many of these compounds have not been reported before in this secretion. Low concentrations of the previously reported hydroxy acids with n-C14, -C15 and -C16 chains were also found. Nicotinate derivatives were applied to the structural determination of glycerol ethers for the first time and shown to reveal the position of methyl branch points in an analogous manner to that previously shown for mono- and di-hydric alcohols. Acids were identified as picolinyl esters.
authors.affiliation: University Department of Pharmacology, Oxford, UK.
authors.forename: D J
authors.initials: DJ
authors.lastname: Harvey
mesh_terms: D000818:Animals; D005227:Fatty Acids / Q000032:analysis; D005233:Fatty Alcohols / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005995:Glyceryl Ethers / Q000032:analysis; D006243:Harderian Gland / Q000737:chemistry*; D006880:Hydroxy Acids / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D008297:Male; D011817:Rabbits
chemical_list: D005227:Fatty Acids; D005233:Fatty Alcohols; D005995:Glyceryl Ethers; D006880:Hydroxy Acids; D008055:Lipids