Expression and induction of intercellular adhesion molecules (ICAMs) and major histocompatibility complex (MHC) antigens on cultured murine oligodendrocytes and astrocytes.
Expression of intercellular adhesion molecule-1 (ICAM-1), ICAM-2-like molecule (Lgp55), and class I/II major histocompatibility complex (MHC) antigens (H-2 and Ia) was investigated in cultures of murine oligodendrocytes and astrocytes. Under unstimulated conditions, low levels of ICAM-1 expression were observed on astrocytes (less than 20%), but not on oligodendrocytes. Lgp55 was expressed intensely on oligodendrocytes (greater than 90%) and to a lesser degree on astrocytes (greater than 70%). A weak class I MHC (H-2) immunoreactivity was identified on both oligodendrocytes and astrocytes (50-70%). Class II MHC (Ia) antigen was undetectable on both cell types. After 48-hr exposure to immune mediators that include interferon-gamma (IFN-gamma), 500 U/ml, and supernatant from concanavalin A (Con A)-activated spleen cells, ICAM-1 expression was markedly increased on astrocytes (greater than 80%), but not on oligodendrocytes. Lgp55 expression on both cell types was not altered. Induction of H-2 antigen expression by immune mediators was quite high on both cell types (greater than 95%), while Ia antigen induction was low on astrocytes (less than 50%) and did not occur on oligodendrocytes. Cell type-specific expression and induction of ICAMs and MHC antigens by immune mediators may play roles in lymphocyte-glial cell interactions at the sites of inflammation in the central nervous system (CNS).
Satoh
J
J
Department of Medicine, University Hospital, Vancouver, B.C., Canada.
Kim
S U
SU
Kastrukoff
L F
LF
Takei
F
F
D000818:Animals; D000911:Antibodies, Monoclonal; D001253:Astrocytes / Q000276:immunology* / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000276:immunology*; D003208:Concanavalin A; D005260:Female; D006183:H-2 Antigens / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000276:immunology; D008285:Major Histocompatibility Complex / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009457:Neuroglia / Q000276:immunology; D009836:Oligodendroglia / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects
D000911:Antibodies, Monoclonal; D015816:Cell Adhesion Molecules, Neuronal; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; D003208:Concanavalin A; D007371:Interferon-gamma