title: The use of synthetic peptides in the formation of biophysically and biologically active pulmonary surfactants.
abstract: Synthetic pulmonary surfactants consisting of mixtures of phospholipids with synthetic peptides based on the amino acid sequence of human surfactant apoprotein SP-B were prepared. These surfactants were analyzed for their ability to lower surface tension on a pulsating bubble surfactometer and for their capacity to improve lung compliance and increase alveolar expansion in a fetal rabbit model of surfactant deficiency. The data demonstrate that several peptides, ranging from 17 to 45 residues in length, matching the carboxy-terminal sequence of the SP-B protein, when appropriately recombined with the phospholipid dipalmitoylphosphatidylcholine and phosphatidylglycerol (3:1), are capable of producing a synthetic surfactant with biophysical and biologic activity approaching that of human surfactant derived from amniotic fluid.
authors.affiliation: Department of Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037.
authors.forename: S D
authors.initials: SD
authors.lastname: Revak
authors.forename: T A
authors.initials: TA
authors.lastname: Merritt
authors.forename: M
authors.initials: M
authors.lastname: Hallman
authors.forename: G
authors.initials: G
authors.lastname: Heldt
authors.forename: R J
authors.initials: RJ
authors.lastname: La Polla
authors.forename: K
authors.initials: K
authors.lastname: Hoey
authors.forename: R A
authors.initials: RA
authors.lastname: Houghten
authors.forename: C G
authors.initials: CG
authors.lastname: Cochrane
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine; D000595:Amino Acid Sequence; D000818:Animals; D001059:Apoproteins / Q000138:chemical synthesis* / Q000494:pharmacology; D008168:Lung / Q000187:drug effects / Q000196:embryology*; D008170:Lung Compliance / Q000187:drug effects*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000494:pharmacology; D010715:Phosphatidylglycerols; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants / Q000138:chemical synthesis* / Q000494:pharmacology; D011817:Rabbits / Q000196:embryology; D013500:Surface Tension
chemical_list: D001059:Apoproteins; D010446:Peptide Fragments; D010715:Phosphatidylglycerols; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants; C046616:pulmonary surfactant apoprotein; D015060:1,2-Dipalmitoylphosphatidylcholine