[Establishment and characterization of a human ureteral cancer cell line producing carbohydrate antigen 19-9 and carcinoembryonic antigen].
We established a new cell line (FU-UrC-1) derived from a human primary ureteral carcinoma xenografted in a nude mouse. This cell line exhibited epithelial characteristics and formed clusters in monolayer cultures. The cells were subcultured in vitro for more than 20 passages and had a doubling time of 53 hours. The modal number of chromosomes was 66. The cell line, which was xenografted again to nude mice, produced tumors essentially identical to the original tumor. Furthermore, the cultured cells expressed carbohydrate antigen 19-9 (CA19-9) and carcinoembryonic antigen (CEA) that were secreted in the culture media. This cell line appears to provide a useful system for studying ureteral carcinoma in vivo and in vitro.
Tanaka
S
S
First Department of Pathology, School of Medicine, Fukuoka University, Japan.
Iwasaki
H
H
Nishida
T
T
Watanabe
R
R
Jouzaki
H
H
Kikuchi
M
M
D000368:Aged; D000782:Aneuploidy; D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis / Q000096:biosynthesis*; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000096:biosynthesis*; D002295:Carcinoma, Transitional Cell / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured; D014516:Ureteral Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology*
D015295:Antigens, Tumor-Associated, Carbohydrate; D002272:Carcinoembryonic Antigen; D004273:DNA, Neoplasm