[Purine and pyrimidine metabolites for the estimation of rumen metabolism: HPLC analysis in milk and blood plasma].
In milk and blood plasma samples of 6 German Simmental and 12 German Black and White heifers it was investigated, whether purine and pyrimidine compounds are suitable indicators of the microbial protein synthesis in the rumen. Therefore the secreted quantities in milk and the concentration in blood plasma are correlated with energy intake. The results indicated significant correlation coefficients for both the secretion quantity of allantoin in milk (r = 0.942) and the concentration of allantoin in blood plasma (r = 0.694). Other investigated compounds appeared more suitable for evaluating the mammary gland metabolism (uridine-lactose synthesis, pseudouridine-protein synthesis). In an experiment with 7 male castrated pigmy goats subjected to a four-day fasting period the decrease of plasma allantoin, which was already apparent after 12 hours of fasting, was closely correlated with the increase of plasma free fatty acids.
Rosskopf
R
R
Institut für Physiologie, Physiologische Chemie und Ernährungsphysiologie, Tierärztliche Fakultät der Ludwig-Maximilians-Universität München.
Rainer
H
H
Giesecke
D
D
D000481:Allantoin / Q000032:analysis / Q000097:blood / Q000378:metabolism; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003562:Cytidine / Q000097:blood; D002149:Energy Intake; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005508:Food Deprivation / Q000502:physiology; D006041:Goats / Q000378:metabolism*; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism; D008892:Milk / Q000737:chemistry* / Q000378:metabolism; D011687:Purines / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D011743:Pyrimidines / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D012417:Rumen / Q000378:metabolism*
D005230:Fatty Acids, Nonesterified; D011687:Purines; D011743:Pyrimidines; D000481:Allantoin; D003562:Cytidine
Purin- und Pyrimidinmetaboliten zur Beurteilung des Pansenstoffwechsels: HPLC-Analysen in Milch und Blutplasma.