title: Cytokine production by the bladder carcinoma cell line 5637: rapid analysis of mRNA expression levels using a cDNA-PCR procedure.
abstract: We have studied cytokine expression by the human bladder carcinoma cell line 5637 using a cDNA-PCR procedure. Transcripts for interleukin-1 alpha (IL-1 alpha), IL-1 beta, IL-6, IL-7, IL-8, granulocyte-macrophage colony-stimulating factor (GM-CSF), G-CSF, M-CSF, tumor-necrosis factor-alpha (TNF-alpha), and TNF-beta were constitutively present, whereas IL-3, IL-4, IL-5, and IL-9 mRNA sequences could not be detected. This expression pattern was not altered after 12-O-tetradecanoyl-phorbol-13-acetate (TPA) stimulation (4 and 8 h) of 5637 cells. Relative expression levels of cytokines were assessed by limiting dilution of the cDNA pool. This procedure proved to be a semiquantitative technique when compared to Northern blot analysis.
authors.affiliation: Department of Hematology, University Medical Center, Leiden, The Netherlands.
authors.forename: J G
authors.initials: JG
authors.lastname: Kaashoek
authors.forename: R
authors.initials: R
authors.lastname: Mout
authors.forename: J H
authors.initials: JH
authors.lastname: Falkenburg
authors.forename: R
authors.initials: R
authors.lastname: Willemze
authors.forename: W E
authors.initials: WE
authors.lastname: Fibbe
authors.forename: J E
authors.initials: JE
authors.lastname: Landegent
mesh_terms: D000199:Actins / Q000235:genetics; D015152:Blotting, Northern; D002460:Cell Line; D016207:Cytokines / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics*; D005091:Exons; D015870:Gene Expression; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics*; D001749:Urinary Bladder Neoplasms
chemical_list: D000199:Actins; D016207:Cytokines; D004273:DNA, Neoplasm; D012333:RNA, Messenger