Nucleotide sequence of the exo-1,3-beta-glucanase-encoding gene, EXG1, of the yeast Saccharomyces cerevisiae.
The nucleotide (nt) sequence of the Saccharomyces cerevisiae gene (EXG1) encoding extracellular exo-1,3-beta-glucanases (EXG) I and II was determined. An open reading frame of 1344 bp codes for a 448-amino acid (aa) polypeptide, with a calculated Mr of 51,307, which contains two potential N-glycosylation sites. The EXG1 DNA hybridizes to a 1.7-kb transcript whose 5' end maps to a position 98 bp upstream from the site of initiation of protein synthesis. Comparison of the N-terminal aa sequence deduced from the nt sequence with that of the purified EXGII revealed the existence of an extra 40-aa peptide in the precursor protein containing a Lys-Arg peptidase-processing site at the junction with the mature, extracellular form. The N-terminal region of the putative precursor is a very hydrophobic segment with structural features resembling those of signal peptides of secreted proteins. The Mr of the mature EXG polypeptide deduced from the nt sequence is 46,385. The 5'- and 3'-flanking regions of the EXG1 gene have structural features in common with other yeast genes.
Vazquez de Aldana
C R
CR
Instituto de Microbiología Bioquímica, Facultad de Biología, CSIC-Universidad de Salamanca, Spain.
Correa
J
J
San Segundo
P
P
Bueno
A
A
Nebreda
A R
AR
Mendez
E
E
del Rey
F
F
D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon; D005800:Genes, Fungal; D043326:Glucan 1,3-beta-Glucosidase; D006031:Glycosylation; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D001617:beta-Glucosidase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism
D003062:Codon; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase