Tissue distribution of mucosal antibody-producing cells specific for respiratory syncytial virus in severe combined immune deficiency (SCID) mice engrafted with human tonsils.
Groups of C.B-17 SCID mice were reconstituted intraperitoneally with human tonsillar mononuclear cells (hu-TMC) from children seropositive for antibody to respiratory syncytial virus (RSV) and subsequently challenged intraperitoneally with inactivated RSV or sham-immunized. The synthesis and the distribution characteristics of human antibody to RSV in various murine tissues were studied using an enzyme-linked immunospot assay (ELISPOT). No specific antibody was observed in sham-immunized animals. In contrast, mice engrafted with hu-TMC exhibited the appearance of specific human antibody secreting cells (hu-ASC) after i.p. immunization with inactivated RSV. RSV-specific hu-ASC were detected only in animals engrafted with cells from donors seropositive for antibodies to Epstein-Barr virus. Hu-TMC engrafted mice showed RSV-specific IgM and, in lower numbers, IgG hu-ASC in several tissues including the lungs. Numbers of RSV-specific IgA hu-ASC were low, however, and detected only in the lung. No RSV-specific hu-ASC were detected in the intestine. These data demonstrate for the first time that hu-TMC-SCID chimeras respond to immunization with viral antigen. Furthermore, the results suggest that hu-TMC engraft in lungs but not in the intestinal tissue.
Nadal
D
D
Department of Pediatrics, State University of New York, Buffalo School of Medicine and Biomedical Sciences.
Albini
B
B
Schläpfer
E
E
Chen
C
C
Brodsky
L
L
Ogra
P L
PL
D000293:Adolescent; D000328:Adult; D000818:Animals; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007132:Immunoglobulin Isotypes / Q000032:analysis; D007153:Immunologic Deficiency Syndromes / Q000276:immunology*; D007422:Intestines / Q000276:immunology; D008168:Lung / Q000276:immunology; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D009092:Mucous Membrane / Q000166:cytology*; D014066:Palatine Tonsil / Q000637:transplantation*; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D014018:Tissue Distribution
D007070:Immunoglobulin A; D007132:Immunoglobulin Isotypes