title: Luminal glucose enhances transepithelial Na+ and fluid transports in rat lungs.
abstract: The effects of luminal glucose on transepithelial Na+ and fluid transports were investigated in rat lungs. Two preparations were used: isolated, perfused lungs and lungs in situ perfused with blood (cross-circulations), a situation more comparable to that existing in vivo. Unidirectional (efflux from air spaces, J(out)) and net (Jnet) Na+ fluxes and fluid absorption from air spaces were estimated in lungs filled with bicarbonate-buffered solutions containing 10 mmol/l of either mannitol, glucose or alpha-methyl-D-glucopyranoside, or 0.1 mmol/l phlorizin in the presence of glucose. In the presence of mannitol J(out) was estimated to be 7.8 +/- 1.02 pmol cm-2 s-1 in isolated lungs and 9.2 +/- 0.97 pmol cm-2 s-1 in lungs in situ, and Jnet 1.0 +/- 0.33 and 2.5 +/- 0.35 pmol cm-2 s-1, respectively. When glucose replaced mannitol J(out) (+ 30% + 40%), Jnet (+200% + 300%) and fluid absorption (+ 100% + 400%) were enhanced in both preparations. Substituting methyl glucoside for mannitol increased Na+ and fluid absorption rates to the same extent as glucose. Phlorizin, in the presence of glucose, reduced Na+ and fluid transports to values similar to those observed in the presence of mannitol. These changes did not result from modifications of the paracellular permeabilities (assessed with [3H]mannitol).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: INSERM U82, Faculté Xavier-Bichat, Paris, France.
authors.forename: G
authors.initials: G
authors.lastname: Saumon
authors.forename: D
authors.initials: D
authors.lastname: Dreyfuss
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D004848:Epithelium / Q000648:ultrastructure; D005947:Glucose / Q000494:pharmacology*; D008168:Lung / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D008757:Methylglucosides / Q000494:pharmacology; D010695:Phlorhizin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000493:pharmacokinetics*
chemical_list: D008757:Methylglucosides; D008353:Mannitol; D012964:Sodium; D010695:Phlorhizin; D005947:Glucose