A G beta protein in the Drosophila compound eye is different from that in the brain.
A G protein beta subunit gene (Gbe) is expressed only in the eyes of adult D. melanogaster. This gene was identified by probing a Drosophila head cDNA expression library with monoclonal antibodies to a previously characterized Drosophila G protein beta subunit (Gbb). Immunoblot and Northern analyses demonstrate that Gbe protein and mRNA is not present in Drosophila mutants that lack eyes. Immunocytochemical and in situ hybridization analyses further demonstrate that Gbe is expressed in the eyes but not in the brain, whereas Gbb is abundantly expressed in the brain. The Gbe product is approximately 45% identical to previously identified G beta subunits and defines a new G beta class. Its localization suggests a possible role in phototransduction.
Yarfitz
S
S
Howard Hughes Medical Institute, University of Washington, Seattle 98195.
Niemi
G A
GA
McConnell
J L
JL
Fitch
C L
CL
Hurley
J B
JB
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D001923:Brain Chemistry; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004331:Drosophila melanogaster; D005123:Eye / Q000737:chemistry; D019204:GTP-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D015870:Gene Expression; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry; D010786:Photoreceptor Cells / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment
D000911:Antibodies, Monoclonal; D009841:Oligonucleotides; D012333:RNA, Messenger; D019204:GTP-Binding Proteins