The relationship between induced reciprocal translocations and cell killing of rhesus monkey spermatogonial stem cells after combined treatments with follicle-stimulating hormone and X-rays.
The induction of reciprocal translocations in spermatogonial stem cells of monkeys (rhesus and crab-eating), visualized in dividing primary spermatocytes, was studied after combined treatments with follicle-stimulating hormone (FSH, 54 IU/kg/week) and X-rays (1 Gy). No clear differences in the frequencies of induced translocations between FSH-pretreated and non-pretreated animals were recorded. Comparison of these translocation data with studies on cell killing in the same monkeys shows that the ratio between the probabilities that radiation-induced basic lesions kill a cell or produce translocations (the p/c ratio) is of the same order of magnitude as that observed for the mouse. Consequently the well documented differences in radiation response between the rhesus monkey and the mouse cannot be explained by differences in the p/c ratio. It is concluded that differences in multiplication-differentiation patterns of surviving stem-cell spermatogonia after irradiation are probably responsible for the observed differences between mice and rhesus monkeys.
van Buul
P P
PP
Department of Radiation Genetics and Chemical Mutagenesis, Sylvius Laboratory, Leiden, The Netherlands.
Seelen
M C
MC
D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008297:Male; D013093:Spermatogonia / Q000187:drug effects* / Q000528:radiation effects; D013737:Testis / Q000528:radiation effects; D014178:Translocation, Genetic / Q000187:drug effects* / Q000528:radiation effects
D005640:Follicle Stimulating Hormone