title: Contractile response to sympathetic innervation in neonatal ventricular cardiomyocytes of the spontaneously hypertensive rat.
abstract: Differences in cardiac development between spontaneously hypertensive rats (SHR) and their normotensive Wistar Kyoto (WKY) controls are observed before the onset of hypertension. To determine whether intrinsic differences in myocardium or autonomic neurons might be responsible for these observations, we studied primary cultures of isolated, never previously innervated ventricular cardiomyocytes from neonatal rats of both strains, and sympathetic innervation was produced by addition of neurons from thoracolumbar sympathetic ganglia. Both same-strain and cross-strain innervation were compared. Amplitude and frequency of contraction were measured from video images of spontaneously contracting cells by on-line video motion analysis. Sympathetic innervation improved contractile function by 61% in SHR cardiomyocytes (p less than 0.001), an effect qualitatively similar to that previously reported for WKY cardiomyocytes. Contractile function of SHR cardiomyocytes cultured without sympathetic explants was 25% less than that of WKY cells (p less than 0.005), but the response of SHR cardiomyocytes to sympathetic innervation was twice as great as that of WKY myocytes (p less than 0.01). Cross-strain innervation experiments showed that sympathetic neurons from both strains were equally effective; interstrain differences were confined to the cardiomyocytes. Interstrain differences in cardiomyocyte contractile function and contractile response to sympathetic innervation are present before the onset of hypertension, and may in part account for alterations in cardiac function observed in prehypertensive SHR.
authors.affiliation: Steele Memorial Children's Research Center, University Heart Center, University of Arizona, Tucson 85724.
authors.forename: T R
authors.initials: TR
authors.lastname: Lloyd
authors.forename: W J
authors.initials: WJ
authors.lastname: Marvin
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D006321:Heart / Q000294:innervation*; D009200:Myocardial Contraction; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D013045:Species Specificity