title: Contribution of the VH11 gene family to mitogen-responsive B cell repertoire in C57BL/6 mice.
abstract: The contribution of VH11 gene family to the development of the primary B cell repertoire has been studied by analyzing 1.8 x 10(4) mitogen induced B lymphocyte colonies. The data demonstrate that VH11 family is predominantly expressed among neonatal splenic as well as adult peritoneal B cell colonies, both rich in Ly-1+ B cells. VH11 gene family expression among B splenocytes decreases during ontogeny and VH11 family pairs stochastically with different V kappa families among mitogen-activated neonatal B cell colonies, which are representative of an antigen unselected B cell repertoire. Thus, an increased VH11 expression among peritoneal and neonatal B cells points towards its biased expression among Ly-1+ B lymphocytes. The restricted V gene rearrangements and VH11-V kappa 9 pairing observed among anti-bromelain-treated mouse red blood cells autoantibodies are likely to be an outcome of both intrinsic gene recombination processes per se as well as selection by an autoantigen and/or local selective environmental factors.
authors.affiliation: Department of Microbiology, Mount Sinai School of Medicine, New York.
authors.forename: A
authors.initials: A
authors.lastname: Kaushik
authors.forename: L
authors.initials: L
authors.lastname: Reininger
authors.forename: G
authors.initials: G
authors.lastname: Kelsoe
authors.forename: J C
authors.initials: JC
authors.lastname: Jaton
authors.forename: C
authors.initials: C
authors.lastname: Bona
mesh_terms: D000367:Age Factors; D000818:Animals; D000916:Antibody Diversity; D001402:B-Lymphocytes / Q000502:physiology*; D015870:Gene Expression; D005803:Genes, Immunoglobulin; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics* / Q000276:immunology; D008934:Mitogens / Q000494:pharmacology; D009693:Nucleic Acid Hybridization
chemical_list: D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D008934:Mitogens