[A titrographic method for determining the glycolysis flow rate with Plasmodium berghei-infected red blood cells].
A procedure is described for the elucidation of the glycolytic flux rate of red blood cells infected with the malarial parasite Plasmodium berghei. It is based on the titration of the protons originating from the glycolytic lactate accumulation. Compared with traditional methods of biochemical measurements of glucose consumption or accumulation of lactate the proposed procedure shows the following advantages: continuously measurement is possible; constancy of the pH-value during the measurement; lower amounts of biological material are necessary (increased sensitivity) increased accuracy; The method can also be applied to studies on other cells and species with normal or increased glycolytic flux rate.
Buckwitz
D
D
Institut für Biochemie, Humboldt-Universität zu Berlin, Bereich Medizin (Charité), FRG.
Jacobasch
G
G
Gerth
C
C
D000818:Animals; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology*; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000032:analysis / Q000378:metabolism; D051379:Mice; D010962:Plasmodium berghei / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
D007773:Lactates; D005947:Glucose
Ein titrographisches Verfahren zur Bestimmung der Glykolyse-Flussrate mit Plasmodium berghei infizierter roter Blutzellen.