title: Enhancement of histological detail using metanil yellow as counterstain in periodic acid Schiff's hematoxylin staining of glycol methacrylate tissue sections.
abstract: Histological detail in sections from tissues embedded in glycol methacrylate was improved by counterstaining PAS/iron-hematoxylin stained sections with a dilute solution of metanil yellow. The addition of the counterstain increases contrast in tissue sections and highlights PAS-positive entities. The staining protocol provides sharp definition of tissue morphology, differentiates cell types and other tissue components and does not produce background staining.
authors.affiliation: Florida Marine Research Institute, Department of Natural Resources, St. Petersburg 33701.
authors.forename: I
authors.initials: I
authors.lastname: Quintero-Hunter
authors.forename: H
authors.initials: H
authors.lastname: Grier
authors.forename: M
authors.initials: M
authors.lastname: Muscato
mesh_terms: D000818:Animals; D001391:Azo Compounds; D005399:Fishes / Q000033:anatomy & histology; D006416:Hematoxylin; D007501:Iron; D010503:Periodic Acid-Schiff Reaction; D011102:Polyhydroxyethyl Methacrylate
chemical_list: D001391:Azo Compounds; D011102:Polyhydroxyethyl Methacrylate; C005108:metanil yellow; D007501:Iron; D006416:Hematoxylin