Functional changes in cultured neurons following transient asphyxia.
Functional responses of cultured neurons from rat cortex and hippocampus to transient hypoxia/asphyxia was studied by the measurement of [3H]2-deoxy-D-glucose (2DG) specific uptake. Hypoxic insult was induced by incubating the cells in 5% CO2/95% N2 or in 1 mM NaCN-containing culture medium for various periods of time. Cell morphology, lactate dehydrogenase efflux and protein levels were also examined. Twenty-four hours following hypoxia (6 to 8 h in an anaerobic atmosphere or 90 min in the presence of NaCN) 2DG specific transport was significantly enhanced, without morphological alteration. Cell injury was evident three days post-hypoxia, with a significantly reduced rate of cellular metabolism. The data suggest indirectly that postanoxic release of excitatory amino acids may be responsible for 'delayed neuronal death'.
Daval
J L
JL
INSERM U.272 24-30, Nancy, France.
Koziel
V
V
Nicolas
F
F
D000818:Animals; D001237:Asphyxia / Q000473:pathology / Q000503:physiopathology*; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D003847:Deoxyglucose / Q000493:pharmacokinetics; D006863:Hydrogen-Ion Concentration; D000860:Hypoxia / Q000378:metabolism / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D010100:Oxygen / Q000378:metabolism; D010313:Partial Pressure; D012966:Sodium Cyanide / Q000494:pharmacology; D013997:Time Factors
D003847:Deoxyglucose; D007770:L-Lactate Dehydrogenase; D012966:Sodium Cyanide; D010100:Oxygen