Detection and enumeration of toxin-producing Pasteurella multocida with a colony-blot assay.
Colonies of toxin-producing Pasteurella multocida were detected with peroxidase-labeled monoclonal antibodies by a membrane assay. Examination of the specificity of the assay with 29 P. multocida cultures representing various geographic origins, hosts, and serotypes indicated that the test was specific for toxin-producing strains. No cross-reactions were observed with Bordetella species that can be associated with P. multocida in producing diseases in animals. A single membrane could be used to assay several isolated strains for toxin production or to enumerate toxin-producing colonies in mixed cultures. Toxin-producing P. multocida colonies were detected in primary cultures; hence, the assay appears to have good potential for widespread application with clinical samples.
Magyar
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Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest, Hungary.
Rimler
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RB
D000818:Animals; D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins / Q000096:biosynthesis*; D001431:Bacteriological Techniques; D015169:Colony Count, Microbial; D007124:Immunoenzyme Techniques; D010325:Pasteurella / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D010326:Pasteurella Infections / Q000175:diagnosis / Q000662:veterinary; D012222:Rhinitis, Atrophic / Q000175:diagnosis / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis
D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins