Complete nucleotide sequence of a genomic clone encoding HLA-B35 isolated from a Caucasian individual of Hispanic origin. Identification of a new variant of HLA-B35.
The primary structure of an HLA class I genomic clone isolated from a homozygous HLA-B35 Caucasian individual of hispanic origin was determined. The nucleotide sequence of exons 1, 2, 4, 5, 6, and 7 is identical to that of the HLA-B35 allele of Oriental origin reported previously. Exon 3 differs in only three nucleotides present in a stretch of 23 bp. These changes introduce three amino acid substitutions in residues 109 (Leu----Phe), 114 (Asp----Asn), and 116 (Ser----Tyr), two of which (114 and 116) are located in one of the beta sheets at the bottom of the peptide binding site. The nature of these replacements in this HLA-B35 variant is likely to affect peptide binding and recognition by T lymphocytes. Introns 1, 2, 3, 4, 5, and 6 from this genomic clone are identical to those present in HLA-Bw58, further confirming the evolutionary origin of HLA-B35.
Chertkoff
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LP
Laboratorio de Inmunogenética, Hospital de Clínicas, Buenos Aires, Argentina.
Herrera
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M
Fainboim
L
L
Satz
M L
ML
D000595:Amino Acid Sequence; D001118:Argentina; D001483:Base Sequence; D005260:Female; D005805:Genes, MHC Class I; D014644:Genetic Variation; D015797:HLA-B35 Antigen / Q000235:genetics*; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D013030:Spain / Q000208:ethnology; D044465:White People / Q000235:genetics
D015797:HLA-B35 Antigen