title: Control of immunoactive inhibin production by human granulosa cells.
abstract: OBJECTIVE
  The aim was to determine the relation between stage of antral follicular development and granulosa cell production of immunoactive inhibin.
  
  
  METHODS
  Primary granulosa cell cultures in serum-free Medium 199 were incubated at 37 degrees C for 96 hours with a change of medium at 48 hours. Inhibin and steroid levels in culture medium were determined by radioimmunoassay. The inhibin assay was based on the N-terminal 1-26 amino acid sequence of the alpha-chain of porcine 32 kDa inhibin using pl alpha 1-26-GLY27-TYR28 as the immunogen, tracer and standard.
  
  
  METHODS
  Granulosa cells were obtained from the ovaries of women with regular menstrual cycles undergoing hysterectomy with unilateral or bilateral oophorectomy to treat non-malignant gynaecological disease.
  
  
  RESULTS
  Basal production of immunoactive inhibin by granulosa cells from presumptive preovulatory follicles (greater than 15 mm diameter) was 5-13 times higher than that by granulosa cells from immature (less than 10 mm diameter) or intermediately mature (10-15 mm diameter) follicles. Basal production of progesterone and oestradiol followed a qualitatively similar pattern, establishing a positive relation between functional granulosa cell maturity and inhibin production. Treatment of granulosa cell cultures from immature follicles with follicle-stimulating hormone (FSH), but not luteinizing hormone (LH), increased inhibin production, time and dose dependently. FSH, but not LH, also brought about similar increases in steroid hormone synthesis by granulosa cells from immature follicles. The stimulatory effect of FSH on granulosa cell inhibin production was augmented at least twofold by the presence of testosterone or 5 alpha-dihydrotestosterone (1.0 mumol/l) but was unaffected by oestradiol. Granulosa cells from intermediately mature follicles undertook variable degrees of both FSH and LH-responsive inhibin production which generally corresponded with gonadotrophin-responsive steroid production. Granulosa cells from presumptive preovulatory follicles showed inconsistent inhibin responses to FSH. However, LH caused marked (at least twofold) increases in inhibin production, paralleling LH-responsive steroid production.
  
  
  CONCLUSIONS
  These results show that for human beings, granulosa cell capacity to produce immunoactive inhibin in vitro increases with follicular maturity. FSH, but not LH, stimulates inhibin production by immature granulosa cells and this response to FSH is subject to modulation by androgen. During preovulatory follicular development, production of inhibin, like steroids, becomes increasingly responsive to LH. Such a development-related pattern of granulosa cell inhibin production helps explain how, post-ovulation, the corpus luteum is able to secrete inhibin as well as steroids. It is also compatible with the concept that locally produced inhibin could participate in the paracrine control of follicular development during the human menstrual cycle.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Edinburgh Centre for Reproductive Biology, Scotland, UK.
authors.forename: S G
authors.initials: SG
authors.lastname: Hillier
authors.forename: E J
authors.initials: EJ
authors.lastname: Wickings
authors.forename: P I
authors.initials: PI
authors.lastname: Illingworth
authors.forename: E L
authors.initials: EL
authors.lastname: Yong
authors.forename: L E
authors.initials: LE
authors.lastname: Reichert
authors.forename: D T
authors.initials: DT
authors.lastname: Baird
authors.forename: A S
authors.initials: AS
authors.lastname: McNeilly
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D005260:Female; D005640:Follicle Stimulating Hormone / Q000502:physiology; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D007265:Inhibins / Q000096:biosynthesis* / Q000276:immunology; D007986:Luteinizing Hormone / Q000502:physiology; D008597:Menstrual Cycle / Q000502:physiology; D008875:Middle Aged; D006080:Ovarian Follicle / Q000502:physiology; D011374:Progesterone / Q000096:biosynthesis; D011863:Radioimmunoassay
chemical_list: D011374:Progesterone; D007265:Inhibins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone