title: Facile detection of anatoxin-a in algal material by thin-layer chromatography with Fast Black K salt.
abstract: A method for facile high-capacity screening of algal samples for anatoxin-a (ANTX-a), a potent neurotoxin of Anabaena flos-aquae, is presented. The method is based on in situ colour reaction of algal extracts containing ANTX-a on a thin-layer chromatographic plate with the diazonium reagent Fast Black K salt, and subsequent separation of the orange-red product. The product, shown to be a stable 3,3-dialkyltriazene, is derived from a reaction involving the aliphatic secondary amino group of ANTX-a. The detection limit for ANTX-a is 10 micrograms g-1 of lyophilized algal material, which is comparable to earlier methods using more complex instrumentation.
authors.affiliation: Department of Forensic Medicine, University of Helsinki, Finland.
authors.forename: I
authors.initials: I
authors.lastname: Ojanperä
authors.forename: E
authors.initials: E
authors.lastname: Vuori
authors.forename: K
authors.initials: K
authors.lastname: Himberg
authors.forename: M
authors.initials: M
authors.lastname: Waris
authors.forename: K
authors.initials: K
authors.lastname: Niinivaara
mesh_terms: D001427:Bacterial Toxins; D002855:Chromatography, Thin Layer / Q000379:methods*; D000458:Cyanobacteria / Q000032:analysis*; D000087522:Cyanobacteria Toxins; D003979:Diazonium Compounds; D007202:Indicators and Reagents; D008387:Marine Toxins / Q000032:analysis*; D052998:Microcystins; D014326:Tropanes
chemical_list: D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D003979:Diazonium Compounds; D007202:Indicators and Reagents; D008387:Marine Toxins; D052998:Microcystins; D014326:Tropanes; C056237:Fast Black K Salt; C509783:anatoxin a