Regulation of the type-II gonadotrophin alpha and beta subunit mRNAs by oestradiol and testosterone in the European eel.
The gonadotrophic function of the European eel (Anguilla anguilla L.) at the silver stage is very weak: gonadotrophin-releasing hormone (GnRH) secretion is deficient and, moreover, dopamine inhibition overrides GnRH action. At the silver stage, oestradiol stimulates the biosynthesis of the type-II gonadotrophin (GTH-II). To study the molecular mechanism of this activation further, we examined the effect of testosterone and oestradiol administration on pituitary levels of mRNA encoding GTH-II alpha and beta subunits. Corresponding eel cDNA probes and Northern blot analysis were used. After 2 weeks, testosterone and oestradiol implantation resulted in a strong increase in mRNA encoding the GTH-II beta subunit (7-fold and 25-fold, respectively) and in a slight, but non-significant, rise in the a subunit mRNA level (1.8-fold and 1.5-fold, respectively). Co-implantation of these two steroids suggested a potentiation of their effects on the beta subunit (104-fold) while an additive effect was indicated on the alpha mRNA level (2.9-fold). Effects were detectable within 4 days and were maximal 4 weeks after implantation. These results indicate that in the European eel at the silver stage, gonadal steroids stimulate differentially the expression of GTH-II subunit genes at a pretranslational level.
Quérat
B
B
Laboratoire de Physiologie Générale et Comparée du MNHN, Unité d'Endocrinologie Comparée du CNRS, URA 90, Paris, France.
Hardy
A
A
Fontaine
Y A
YA
D000811:Anguilla / Q000235:genetics*; D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism; D006065:Gonadotropins, Pituitary / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D013739:Testosterone / Q000494:pharmacology*
D006065:Gonadotropins, Pituitary; D012333:RNA, Messenger; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D004958:Estradiol