title: Posttranscriptional regulation of interferon mRNA levels in peritoneal macrophages.
abstract: Low levels of beta interferon (IFN) mRNA are transcribed in freshly explanted murine peritoneal macrophages. Nuclear runoff transcription assays show that this "constitutive" IFN-beta-mRNA transcription does not increase in macrophages treated either with lipopolysaccharide or with IFN-gamma, which induce a marked accumulation of this mRNA and greatly increase IFN secretion. Therefore, these agents promote accumulation of IFN-beta mRNA by posttranscriptional mechanisms. The IFN-alpha 2 gene is also constitutively transcribed by macrophages, but the corresponding mRNA does not accumulate in lipopolysaccharide-treated cells.
authors.affiliation: Laboratorio di Virologia, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Gessani
authors.forename: P
authors.initials: P
authors.lastname: Di Marzio
authors.forename: P
authors.initials: P
authors.lastname: Rizza
authors.forename: F
authors.initials: F
authors.lastname: Belardelli
authors.forename: C
authors.initials: C
authors.lastname: Baglioni
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D007370:Interferon Type I / Q000235:genetics*; D007371:Interferon-gamma / Q000494:pharmacology; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D012323:RNA Processing, Post-Transcriptional / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D007370:Interferon Type I; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D007371:Interferon-gamma