Moloney leukemia virus immortalizes B lymphocytes in vitro.
An in vitro culture system in which Moloney murine leukemia virus induces immortalization of mature B lymphocytes has been developed. The cell lines derived in this way are nontumorigenic, and virus production is not required to sustain them. This system provides a new in vitro model with which to study the stepwise process of transformation by retroviruses lacking oncogenes.
Runnels
J
J
Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts 02111.
Serunian
L
L
Thursby
M
M
Rosenberg
N
N
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects; D001854:Bone Marrow Cells; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics*; D013997:Time Factors; D014764:Viral Proteins / Q000096:biosynthesis* / Q000302:isolation & purification
D008070:Lipopolysaccharides; D014764:Viral Proteins