Effects of human recombinant macrophage colony-stimulating factor on the secretion of lipoprotein lipase from macrophages.
The effects of human recombinant macrophage colony-stimulating factor (M-CSF) on the secretion of lipoprotein lipase were studied in rat alveolar macrophages. Five nanograms per milliliter M-CSF significantly enhanced lipoprotein lipase secretion (threefold), and the maximal effect (10-fold) of M-CSF on lipoprotein lipase secretion was observed at a dose of 200 ng/ml M-CSF. The effect of M-CSF was time dependent but was not manifested during the first 8 hours of incubation. After 24 hours, its effects were evident and dose dependent. On blot hybridization of macrophage RNAs with human cDNA of lipoprotein lipase, a remarkable and dose-dependent increase in mRNA level (7.3-fold) was found in M-CSF-treated alveolar macrophages. The secretion of lipoprotein lipase was also enhanced in human monocyte-derived macrophages (2.6-fold), whereas the secretion from either THP-1 cells, P388 cells, or J774 cells was not significantly enhanced. These results indicate that the stimulation of lipoprotein lipase secretion after M-CSF treatment was evident in rat alveolar macrophages and human monocyte-derived macrophages on the basis of both enzyme activity and mRNA level; therefore, M-CSF may be involved in lipoprotein metabolism of macrophages through modulation of the secretion of lipoprotein lipase.
Mori
N
N
Third Department of Internal Medicine, University of Tokyo, Hongo, Japan.
Gotoda
T
T
Ishibashi
S
S
Shimano
H
H
Harada
K
K
Inaba
T
T
Takaku
F
F
Yazaki
Y
Y
Yamada
N
N
D000818:Animals; D004247:DNA / Q000032:analysis; D066298:In Vitro Techniques; D008071:Lipoprotein Lipase / Q000378:metabolism*; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D008264:Macrophages / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011506:Proteins / Q000032:analysis; D011650:Pulmonary Alveoli / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000494:pharmacology
D011506:Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D016173:Macrophage Colony-Stimulating Factor; D004247:DNA; D008071:Lipoprotein Lipase