title: Sulfated O-glycoproteins secreted by guinea pig trachea in organ culture.
abstract: Organ culture of guinea pig trachea was performed in the presence of [35S]sulfate in order to characterize the sulfated glycoproteins released from the respiratory epithelium and mucosa. The sulfated macromolecules that were synthesized during a 6-h incorporation were separated by CsBr density-gradient centrifugation and gel-filtration chromatography successively. Most of the sulfated secreted macromolecules corresponded to a population of glycoproteins sensitive to reductive beta-elimination but resistant to both chondroitinase ABC and heparinase. These glycoproteins had different buoyant densities (ranging from 1.48 g/ml to 1.16 g/ml) and could be subfractionated according to molecular mass. A major part of the radioactivity was incorporated into high-molecular-mass mucins that were excluded from a Sepharose CL-2B column and did not penetrate into polyacrylamide gel in PAGE. However, a mixture of sulfated O-glycoproteins of much lower molecular mass was also characterized in addition to low amounts of chondroitin sulfate. Epithelial goblet cells are the predominant mucin-containing cells of the respiratory guinea pig trachea. Our results suggest that a wide range of sulfated O-glycoproteins are secreted by the guinea pig tracheal mucosa.
authors.affiliation: Unité INSERM No. 16, Lille, France.
authors.forename: H
authors.initials: H
authors.lastname: Rahmoune
authors.forename: H P
authors.initials: HP
authors.lastname: Rounding
authors.forename: W J
authors.initials: WJ
authors.lastname: McDonald-Gibson
authors.forename: G
authors.initials: G
authors.lastname: Lamblin
authors.forename: R L
authors.initials: RL
authors.lastname: Hall
authors.forename: P
authors.initials: P
authors.lastname: Roussel
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D002808:Chondroitin Lyases / Q000378:metabolism; D002809:Chondroitin Sulfates / Q000378:metabolism; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006001:Glycoconjugates / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006026:Glycoside Hydrolases; D006168:Guinea Pigs; D019763:Heparin Lyase; D006863:Hydrogen-Ion Concentration; D008297:Male; D009092:Mucous Membrane / Q000378:metabolism; D009924:Organ Culture Techniques; D011133:Polysaccharide-Lyases / Q000378:metabolism; D014132:Trachea / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D006001:Glycoconjugates; D006023:Glycoproteins; D002809:Chondroitin Sulfates; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase; D002808:Chondroitin Lyases; D011133:Polysaccharide-Lyases; D019763:Heparin Lyase