Identification and sequence analysis of the hupR1 gene, which encodes a response regulator of the NtrC family required for hydrogenase expression in Rhodobacter capsulatus.
The hupR1 gene from Rhodobacter capsulatus was cloned and sequenced. It can encode a protein of 53,843 Da which shares significant similarity with several transcriptional regulators and activates transcription of the structural hupSL genes of [NiFe]hydrogenase, as shown by the use of a translational fusion of lacZ with the hupSL promoter. A Hup- mutant having a point mutation in the hupR1 gene is described.
Richaud
P
P
Laboratoire de Biochimie Microbienne (Centre National de la Recherche Scientifique Unité 1130 alliée à l'Institut National de la Santé de la Recherche Médicale), Grenoble, France.
Colbeau
A
A
Toussaint
B
B
Vignais
P M
PM
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006864:Hydrogenase / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D015183:Restriction Mapping; D016354:Rhodobacter capsulatus / Q000235:genetics*; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D014157:Transcription Factors; C070743:hupR1 protein, Rhodobacter capsulatus; D006864:Hydrogenase