Spectra of voltage-sensitive fluorescence of styryl-dye in neuron membrane.
The voltage sensitivity of fluorescence of an aminobenzstyryl-pyridinium dye (di4-ANEPPS) is characterized in Retzius cells dissociated from the leech. The modulation of the complete spectra of excitation and emission is determined. The spectral changes induced by depolarization are described by a blue shift of the absorption spectrum, by a weaker blue shift and an enhanced width of the fluorescence spectrum and by a decrease of the yield of fluorescence. These changes are attributed tentatively to a superposition of electrochromism and of field-induced resolvation.
Fromherz
P
P
Abteilung Biophysik der Universität Ulm, Ulm-Eselsberg, Germany.
Lambacher
A
A
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D007865:Leeches / Q000737:chemistry; D008564:Membrane Potentials; D009474:Neurons / Q000737:chemistry; D011726:Pyridinium Compounds / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; C050019:1-(3-sulfonatopropyl)-4-(beta)(2-(di-n-butylamino)-6-naphthylvinyl)pyridinium betaine