title: Detection apparatus for multiple heterogeneous chemiluminescence immunoassay configurations.
abstract: We describe an apparatus for measuring signals emanated from two heterogeneous chemiluminescence immunoassay (CLIA) configurations: antibody-coated polystyrene beads, in reaction tray wells, and microparticles captured by a porous matrix. An optics and fluidics design which allows the use of a common detection head for these two different assay configurations is described. The detection head moves along three Cartesian coordinates to create a localized light-tight compartment around each individual disposable reaction vessel. Reproducibility of the light seal, trigger solution delivery, and mixing is achieved for acridinium-labeled CLIA. The coated polystyrene beads configuration is tested using beta HCG, CEA, and TSH assays. The microparticle-capture configuration is tested using beta HCG and HBsAg assays. The microparticle capture CLIA has shorter incubation times and the potential for ease of automation.
authors.affiliation: Diagnostics Division, Abbott Laboratories, Abbott Park, Illinois 60064.
authors.forename: O S
authors.initials: OS
authors.lastname: Khalil
authors.forename: T F
authors.initials: TF
authors.lastname: Zurek
authors.forename: C
authors.initials: C
authors.lastname: Pepe
authors.forename: K
authors.initials: K
authors.lastname: Genger
authors.forename: D G
authors.initials: DG
authors.lastname: Huff
authors.forename: C
authors.initials: C
authors.lastname: Coleman
authors.forename: C
authors.initials: C
authors.lastname: Hanna
authors.forename: R
authors.initials: R
authors.lastname: Hu
authors.forename: J
authors.initials: J
authors.lastname: Mackowiack
authors.forename: L
authors.initials: L
authors.lastname: Bennett
mesh_terms: D003627:Data Interpretation, Statistical; D004867:Equipment Design; D006511:Hepatitis B Antigens / Q000032:analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D008163:Luminescent Measurements; D011137:Polystyrenes; D012044:Regression Analysis
chemical_list: D006511:Hepatitis B Antigens; D011137:Polystyrenes