title: Vero cells stimulate human sperm motility in vitro.
abstract: OBJECTIVE
  To study human sperm motility in a coculture system for in vitro fertilization (IVF).
  
  
  METHODS
  We studied the viability and motility (percentage and curvilinear velocity) of human spermatozoa after incubation in: (1) medium 199 supplemented with fetal calf serum (M199/FCS) together with Vero cells; (2) Vero cell conditioned M199/FCS; (3) M199/FCS supplemented with Vero cell extract; and (4) some control media. In a second experiment, FCS was substituted by sera from different IVF patients.
  
  
  METHODS
  Semen samples were obtained from the fertility laboratory of the St. Radbound Hospital, Nijmegen, The Netherlands.
  
  
  METHODS
  Twelve men of couples with fertility problems.
  
  
  METHODS
  None.
  
  
  METHODS
  The motility parameters were determined with a computerized motility-analyzing system, after 24 hours' incubation at 37 degrees C and 5% CO2. Viability was determined after eosin Y staining.
  
  
  RESULTS
  Sperm viability was the same in all media. In the coincubation system, the Vero cell-conditioned medium, and the experiment with human sera, the sperm motility parameters were higher (P less than 0.005) than in the control media. Vero cell extract did not have this positive effect.
  
  
  CONCLUSIONS
  Coincubation has a positive effect on sperm motility and may be beneficial to IVF, intrauterine insemination, and artificial insemination.
authors.affiliation: Laboratory of Endocrinology and Reproduction, St. Radbound Hospital, Catholic University Nijmegen, The Netherlands.
authors.forename: A M
authors.initials: AM
authors.lastname: Wetzels
authors.forename: B A
authors.initials: BA
authors.lastname: Bastiaans
authors.forename: H J
authors.initials: HJ
authors.lastname: Goverde
authors.forename: H J
authors.initials: HJ
authors.lastname: Janssen
authors.forename: R
authors.initials: R
authors.lastname: Rolland
mesh_terms: D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology; D014709:Vero Cells / Q000502:physiology*
chemical_list: D003470:Culture Media