title: Sulfhydryl groups are involved in the interaction of FSH with its receptor.
abstract: FSH has recently been reported to possess thioredoxin-like activity, presumably explained by the homology between a region of FSH-beta subunit and the active site of thioredoxin. The homologous sequence lies within a receptor binding region, which suggests a possible role for sulfhydryl groups in the formation of an active hormone-receptor complex and subsequent signal transduction. In order to determine the relevance of sulfhydryl groups on FSH-receptor interaction, we studied the effect of N-ethylmaleimide (NEM) and glutathione on FSH binding. The results indicate that free sulfhydryl groups, probably derived from the FSH receptor, are involved in ligand-receptor interaction.
authors.affiliation: Department of Biochemistry, Albany Medical College, NY 12208.
authors.forename: T A
authors.initials: TA
authors.lastname: Santa-Coloma
authors.forename: P
authors.initials: P
authors.lastname: Grasso
authors.forename: L E
authors.initials: LE
authors.lastname: Reichert
mesh_terms: D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D005033:Ethylmaleimide / Q000494:pharmacology*; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D005978:Glutathione / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008297:Male; D011485:Protein Binding; D011962:Receptors, FSH / Q000187:drug effects / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013737:Testis / Q000378:metabolism*
chemical_list: D011962:Receptors, FSH; D013438:Sulfhydryl Compounds; D005640:Follicle Stimulating Hormone; D005978:Glutathione; D005033:Ethylmaleimide