[Separation and quantitative determination of three saponins in licorice root by high performance liquid chromatography].
A new analytical method for the separation and simultaneous determination of three saponins in Licorice root by high performance liquid chromatography has been developed. The saponins, glycyrrhizic acid (S-I), uralsaponin B (S-II) and uralsaponin A (S-III) were separated and determined by use of the solvent system CH3CN-3% HOAc (H2O) (47:53) at 248 nm, and with gradient increasing flow rate, the operation can be completed in 20 min. This method is sensitive and acuate with good reproducibility. The contents of three saponins in Chinese Licorice roots derived from four Glycyrrhiza species were determined.
Zeng
L
L
School of Pharmaceutical Sciences, Beijing Medical University.
Zhang
R Y
RY
Lou
Z C
ZC
D000890:Anti-Infective Agents; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D006035:Glycyrrhiza / Q000032:analysis*; D019695:Glycyrrhizic Acid; D010946:Plants, Medicinal; D014315:Triterpenes / Q000032:analysis* / Q000302:isolation & purification
D000890:Anti-Infective Agents; D004365:Drugs, Chinese Herbal; D014315:Triterpenes; C051422:uralsaponin A; C051423:uralsaponin B; D019695:Glycyrrhizic Acid; D006034:Glycyrrhetinic Acid