Role of oxygen free radicals in respiratory distress induced by arachidonic acid in the rat.
The purpose of our present study is the possible implication of oxygen free radicals in the respiratory distress induced in rats by intravenous administration of arachidonic acid (20 mg/kg). The respiratory frequency was measured and plasma TXB2 concentration was assayed by RIA from blood withdrawn 1 min after arachidonic acid administration. The substances studied were: SOD, catalase, mannitol, DMSO, BHT, imidazole. All the drugs, except imidazole, significantly protect the rats from the respiratory distress induced by arachidonic acid. SOD, catalase, BHT and imidazole inhibit whereas mannitol and DMSO increase the plasma levels of TXB2. We suggest that oxygen free radicals generated in the respiratory burst induced by arachidonic acid are mainly responsible for the consequent respiratory distress.
Puig-Parellada
P
P
Unitat de Farmacologia, Terapèutica i Farmacologia Clinica, Facultat de Medicina, Universitat de Barcelona, Spain.
Sánchez
J
J
Carbonell
L
L
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000037:antagonists & inhibitors*; D016166:Free Radical Scavengers; D005609:Free Radicals; D010100:Oxygen / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012131:Respiratory Insufficiency / Q000139:chemically induced / Q000503:physiopathology* / Q000517:prevention & control
D001095:Arachidonic Acids; D016166:Free Radical Scavengers; D005609:Free Radicals; D016718:Arachidonic Acid; D010100:Oxygen