B-Z transition of poly(dG-m5dC) induced by binding of Lys-containing peptides.
Effects of oligopeptides containing Lys residues on the conformation of poly(dG-m5dC) have been investigated by circular dichroism spectroscopy. Lys-Ala-Lys (KAK) and its longer analogs with Lys-Ala repeats are found to convert the B-form polynucleotide to the Z form very efficiently. The ability to induce the B-Z transition is characteristic of alternating Lys-Ala sequences and increases exponentially with increasing number of the repeats. The heptapeptide KAKAKAK has an ability comparable with that of spermine, one of the most effective inducers hitherto known. The present results provide the first example of the B-Z transition of poly(dG-m5dC) induced by peptide binding.
Takeuchi
H
H
Pharmaceutical Institute, Tohoku University, Sendai, Japan.
Hanamura
N
N
Hayasaka
H
H
Harada
I
I
D000409:Alanine; D000595:Amino Acid Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D066298:In Vitro Techniques; D008239:Lysine / Q000737:chemistry*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000737:chemistry*; D011089:Polydeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship
D004268:DNA-Binding Proteins; D010455:Peptides; D011089:Polydeoxyribonucleotides; C029988:poly(dG-m(5)dC).poly(dG-m(5)dC); D004247:DNA; D008239:Lysine; D000409:Alanine