New shuttle vectors for Haemophilus influenzae and Escherichia coli: P15A-derived plasmids replicate in H. influenzae Rd.
With the aim of identifying new plasmids useful for molecular cloning in Haemophilus influenzae, several P15A-derived plasmids were tested for their ability to transform H. influenzae Rd. The four plasmids tested, pACYC177, pACYC184, pSU2718, and pSU2719 were all able to establish in H. influenzae Rd. The plasmids were stable, could be purified by standard protocols, and were compatible with a plasmid carrying the RSF0885 origin of replication.
Chandler
M S
MS
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
D003001:Cloning, Molecular / Q000379:methods; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D006193:Haemophilus influenzae / Q000235:genetics*; D010957:Plasmids; D014169:Transformation, Bacterial