Genotoxic effect of the antitumour agent homo-aza-steroidal ester of p-bis-(2-chloroethyl)aminophenoxy acetic acid (NSC 294859) tested in the Drosophila wing somatic mutation and recombination test.
The genotoxic activity of the new antineoplastic alkylating agent 3 beta-hydroxy-13 alpha-amino-13,17-seco-5 alpha-androstan-17-oic-13,17- lactam-p-bis-(2-chloroethyl) aminophenoxyacetate (NSC 294859) was studied in the somatic mutation and recombination test (SMART) in Drosophila melanogaster. The drug was administered to larvae which were transheterozygous for two recessive wing cell markers mwh and flr3. Several concentrations were tested and two of them showed a clear induction of mosaic spots, expressing the mutant phenotypes. Multiple wing hair (mwh) spots were the main type observed but twin spots and flares (flr3) spots were also detected. We conclude that the compound induces both mutations and somatic recombination in the SMART test.
Department of Biology, University of Patras, Greece.
G
G
Stephanou
N A
NA
Demopoulos
P
P
Catsoulacos
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D004331:Drosophila melanogaster / Q000235:genetics; D005260:Female; D005819:Genetic Markers; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens; D009154:Mutation; D009588:Nitrogen Mustard Compounds / Q000633:toxicity*; D011995:Recombination, Genetic; D014921:Wings, Animal
D000970:Antineoplastic Agents; D005819:Genetic Markers; D009153:Mutagens; D009588:Nitrogen Mustard Compounds; C038583:NSC 294859