Effect of glucose concentration, agar, and amount of inoculum on the 6.5% sodium chloride tolerance test for presumptive identification of enterococci.
The 6.5% NaCl tolerance test is simple and commonly used for presumptive identification of enterococci; however, it is not strictly standardized. The effect of glucose concentration, types of media and the size of inocula on the growth of enterococci have been evaluated. The results showed that broth was superior to agar media for enterococcal growth at any amount of inoculum. The media containing 1.0% glucose also showed superiority to media containing 0.1% glucose. It is suggested that broth containing 1.0% glucose be the medium of choice for 6.5% NaCl tolerance test.
Department of Medical Technology, National Cheng-Kung University Medical Center, Tainan, Taiwan, R.O.C.
J C
JC
Chang
D000362:Agar; D001431:Bacteriological Techniques; D003470:Culture Media; D016983:Enterococcus / Q000254:growth & development / Q000302:isolation & purification*; D005947:Glucose / Q000008:administration & dosage*; D012965:Sodium Chloride / Q000008:administration & dosage
D003470:Culture Media; D012965:Sodium Chloride; D000362:Agar; D005947:Glucose