Plasmodium falciparum: the effect of pH and Ca2+ concentration on the in vitro cytoadherence of infected erythrocytes to amelanotic melanoma cells.
Cytoadherence of Plasmodium falciparum-infected erythrocytes to amelanotic melanoma cells was pH dependent; increased adherence was observed in the pH range of 6.1 to 6.8 and was greatest between pH 6.6 and 6.8 Ca2+ promoted cytoadherence, but at higher concentrations (40-50 mM) than is usually the case for cell-cell adhesion. The effects of pH and Ca2+ were interdependent--the pH optimum of cytoadherence was altered by the Ca2+ concentration in the medium. The adherent properties of several P. falciparum lines (including a knobless cytoadherent line) under varying pH and Ca2+ concentrations were similar.
University of California, Department of Biology, Riverside 92521.
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Crandall
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Smith
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Sherman
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D004912:Erythrocytes / Q000469:parasitology* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008545:Melanoma; D010963:Plasmodium falciparum / Q000502:physiology*; D014407:Tumor Cells, Cultured
D002118:Calcium