[Evaluation of a radioimmunologic technique for the detection of anti-native DNA antibodies].
The anti-native DNA antibodies were measured by a radioimmunoassay (RIA) type Farr assay in the sera from 648 patients: 108 with active or inactive systemic lupus erythematosus (SLE), 181 with clinical symptoms of another connective tissue disease, 171 with liver diseases, 29 with different pathology and 159 normal sera were obtained from a blood bank. The anti-DNA kit has been calibrated against the first international units/ml. This assay has proved to be sensitive and specific, and appears to be reliable for the diagnosis and follow-up of SLE patients. The authors propose a new reference cut-off level higher than producer's one.
Laboratoire Central d'Immunologie et d'Hématologie, Hôpital Saint-Antoine, Paris.
C
C
Johanet
E
E
Soulie
Y B
YB
Absalon
T
T
Ocwieja
N
N
Abuaf
D000974:Antibodies, Antinuclear / Q000032:analysis*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis; D011863:Radioimmunoassay / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity
D000974:Antibodies, Antinuclear
Evaluation d'une technique radio-immunologique pour la détection des anticorps anti-ADN natif.