Involvement of OP18 in cell proliferation.
Op18 is a highly conserved major cytosolic phosphoprotein that is expressed at high levels in acute leukemia and in neuroblastoma. In this study we present evidence pointing to a role for Op18 in cellular proliferation. Blocking of Op18 mRNA translation using antisense oligonucleotides delayed entrance of mitotically stimulated normal peripheral blood lymphocytes into the S phase. Moreover treatment of HL-60 promyelocytic leukemia cells with DMSO or PMA which induced terminal differentiation resulted in a decrease in the level of Op18 RNA and protein. Inhibition of lymphoid proliferation with cyclosporin also resulted in reduced Op18 levels.
Melhem
R F
RF
Department of Pediatrics, University of Michigan Medical School, Ann Arbor 48109-0510.
Strahler
J R
JR
Hailat
N
N
Zhu
X X
XX
Hanash
S M
SM
D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008868:Microtubule Proteins; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000502:physiology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010750:Phosphoproteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics; D050777:Stathmin; D013755:Tetradecanoylphorbol Acetate; D014407:Tumor Cells, Cultured
D008868:Microtubule Proteins; D009363:Neoplasm Proteins; D016376:Oligonucleotides, Antisense; D010750:Phosphoproteins; D012333:RNA, Messenger; C493995:STMN1 protein, human; D050777:Stathmin; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide