Effect of Clostridium perfringens alpha toxin on contraction of isolated guinea-pig diaphragm.
The effect of Clostridium perfringens alpha toxin on contraction induced by electric stimulation of isolated guinea-pig diaphragm was investigated. The toxin inhibited electrically stimulated contraction of the tissue in a dose- and incubation time-dependent manner. Tetrodotoxin resulted in no effect of the action of the toxin. Nifedipine dose-dependently delayed the action of the toxin, but verapamil and diltiazem did not. On the other hand, treatment of the toxin with N-acetylimidazole caused significant reduction of the inhibitory activity of the toxin on contraction, but did not cause significant loss of phospholipase C activity (PN activity) as measured by hydrolysis of p-nitrophenylphosphorylcholine. The data showed that the toxin impairs contraction of isolated guinea-pig diaphragm.
Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University.
J
J
Sakurai
Y
Y
Tsuchiya
S
S
Ochi
Y
Y
Fujii
D000818:Animals; D001427:Bacterial Toxins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002135:Calcium-Binding Proteins; D003016:Clostridium perfringens; D003964:Diaphragm / Q000187:drug effects / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D006168:Guinea Pigs; D007093:Imidazoles / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D013779:Tetrodotoxin / Q000494:pharmacology; D010738:Type C Phospholipases / Q000378:metabolism
D001427:Bacterial Toxins; D002121:Calcium Channel Blockers; D002135:Calcium-Binding Proteins; D007093:Imidazoles; D013779:Tetrodotoxin; D010738:Type C Phospholipases; C040649:alpha toxin, Clostridium perfringens; C012160:N-acetylimidazole