Immune response to HIV p24 core protein during the early phases of human immunodeficiency virus infection.
The immune response to the p24 core antigen of human immunodeficiency virus type 1 (HIV-1) was studied in serial samples collected prospectively from 52 homosexual males in two separate cohorts from Amsterdam and San Francisco. p24 antibody levels were quantified with an antigen sandwich immunoassay using p24 recombinant antigen as capture and probe. Titers and slopes of dilution curves reflecting antibody affinity were analyzed. Only 45 of 52 men developed a measurable primary immune response to p24. In 17 (33%) patients there was a low response with maximum antibody titer below 66, shallow (low affinity) dilution curve, and 10 of the 17 became HIV antigen positive over a 2 year period. In 24 (46%) of the 52 patients titers ranged from 100-4000, steeper dilution curves were noted, and none became HIV antigen positive. Four (8%) men developed a strong immune response with high titers (greater than 12,000) and high affinity type dilution curve. Over time, after the peak immune response, antibody titer declined in some individuals related in part to the formation of immune complexes between HIV-1 p24 antigen and antibody which were dissociable. In vitro, the addition of increasing amounts of purified p24 antigen corresponded to decreasing antibody titer and a shallower dilution curve suggesting a preferential consumption of high affinity antibodies for complex formation. The magnitude of immune response to HIV-1 p24 antigen varies widely in infected homosexual men. Both the intrinsic ability to mount an immune response and immune complex formation contribute to the measurable antibody level.
Department of Research and Development and Medical Research, Abbott Laboratories, Abbott Park, IL.
B
B
McRae
J A
JA
Lange
M S
MS
Ascher
F
F
de Wolf
H W
HW
Sheppard
J
J
Goudsmit
J P
JP
Allain
D001727:Bisexuality; D015331:Cohort Studies; D015483:HIV Antibodies / Q000097:blood; D015488:HIV Antigens / Q000097:blood; D016655:HIV Core Protein p24 / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology; D006716:Homosexuality; D006801:Humans; D008297:Male
D015483:HIV Antibodies; D015488:HIV Antigens; D016655:HIV Core Protein p24