In vivo 31P nuclear magnetic resonance spectroscopy of experimental murine tumours and human tumour xenografts: effects of blood flow modification.
The effect of hydralazine on tumours appears to vary depending on tumour type. Blood flow and radiation sensitivity decrease more in murine tumours than human tumour xenografts. In this study a comparison between various tumour types has been made using in vivo 31P nuclear magnetic resonance spectroscopy (NMRS) to follow the metabolic responses occurring after clamping or intravenous administration of hydralazine (5 mg kg-1). Large increases in the Pi/total phosphate ratio were found with the murine sarcomas, KHT and RIF-1 implanted into C3H/He mice. However little or no effect was seen for the two human xenografted tumours, HX118 and HT29 implanted in MFI nu/nu/01a mice. An intermediate response was observed for KHT tumours grown in nu/nu mice. All tumours showed a large response to clamping. The anaesthetic Hypnorm/Hypnovel has a great influence on the response of the tumour metabolism to hydralazine appearing to both prolong and increase the changes induced. There is evidence to support the theory that the changes in 31P spectra are related to the oxygen status of the tumours.
MRC Radiobiology Unit, Chilton, Didcot, UK.
J C
JC
Bremner
C J
CJ
Counsell
G E
GE
Adams
I J
IJ
Stratford
P J
PJ
Wood
J F
JF
Dunn
G K
GK
Radda
D000818:Animals; D003110:Colonic Neoplasms / Q000098:blood supply* / Q000188:drug therapy; D006801:Humans; D006830:Hydralazine / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008546:Melanoma, Experimental / Q000098:blood supply* / Q000188:drug therapy; D051379:Mice; D008809:Mice, Inbred C3H; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010758:Phosphorus; D012039:Regional Blood Flow / Q000187:drug effects; D012513:Sarcoma, Experimental / Q000098:blood supply* / Q000188:drug therapy; D014183:Transplantation, Heterologous
D006830:Hydralazine; D010758:Phosphorus