Exogenous GM3 ganglioside stimulates process formation and glycoprotein release by cultured bovine oligodendrocytes.
Isolated adult bovine oligodendrocytes maintained in vitro for 10 days were treated for 1 day with 50 micrograms/ml of GM3 ganglioside (NeuNac alpha 2-3Gal beta 1-4Glc beta 1-1'ceramide) in serum-free culture medium. The treated oligodendrocytes had significantly longer processes with more branching than control cells in the same medium without GM3. The treatment also stimulated the release of a series of 22-100-kDa, [3H]glucosamine-labeled glycoproteins into the culture medium. Treatment of oligodendrocytes maintained in vitro for 50 days with GM3 for 1 day resulted in a thickening of the processes and the appearance of many fine branches on existing processes as well as a similar stimulation of glycoprotein release into the medium.
Laboratory of Molecular and Cellular Neurobiology, NINDS, NIH, Bethesda, Maryland 20892.
S H
SH
Yim
E
E
Yavin
J A
JA
Hammer
R H
RH
Quarles
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D005679:G(M3) Ganglioside / Q000494:pharmacology*; D006023:Glycoproteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure
D005679:G(M3) Ganglioside; D006023:Glycoproteins; D009419:Nerve Tissue Proteins