IgE and IgG binding of peptides expressed from fragments of cDNA encoding the major house dust mite allergen Der p I.
Large peptides expressed from cDNA fragments of a clone encoding the mite allergen Der p I were able to bind IgE and IgG in sera from allergic individuals. The binding was found for peptides from sequences throughout the molecule, with at least five regions, comprising residues 1-56, 53-99, 98-140, 166-194, and 188-222. The only limitation was that more than 30 amino acid residues were required for consistent binding. Each of seven sera examined showed a different profile of antibody binding to the peptides. For the most part the pattern of IgE and IgG binding to the peptides for each serum was similar, demonstrating a concordant repertoire. In 5/7 sera, however, IgG bound to some peptides which had little or no IgE binding activity, thus showing more diverse specificities. It is suggested that some divergence of repertoire can develop during the maturation of the B cell response.
Western Australian Research Institute for Child Health, Princess Margaret Hospital, Perth.
W K
WK
Greene
J G
JG
Cyster
K Y
KY
Chua
R M
RM
O'Brien
W R
WR
Thomas
D000485:Allergens / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D000818:Animals; D039741:Antigens, Dermatophagoides; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism*; D066298:In Vitro Techniques; D008925:Mites / Q000276:immunology*; D010455:Peptides / Q000276:immunology* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000276:immunology; D015183:Restriction Mapping
D000485:Allergens; D039741:Antigens, Dermatophagoides; D007074:Immunoglobulin G; D010455:Peptides; D011993:Recombinant Fusion Proteins; D007073:Immunoglobulin E; D004247:DNA