Role of interferon-gamma on the in vivo expression of functional interleukin-2 receptors by murine macrophages.
Interferon-gamma activates both in vitro and in vivo macrophage functions. Injection of rat recombinant interferon-gamma (rR-IFN-gamma) induced the expression of interleukin-2 receptors (IL-2R) by peritoneal macrophages from normal BALB/c and MRL-+/+ mice. Moreover, rR-IFN-gamma stimulated in a dose-dependent manner the oxidative burst of cells as revealed by luminol-dependent chemiluminescene (LDCL). Resident peritoneal macrophages from MRL-lpr/lpr (mice that develop a systemic lupus-like syndrome) showed a higher PMA-triggered LDCL response. This enhanced activity was accompanied by an increase in IL-2R expression (30% vs. less than 1%). The "activated" macrophages from rR-IFN-gamma-treated normal mice as well as MRL-lpr/lpr mice did not respond to the addition of recombinant interleukin-2 (rHu-IL-2) by an increase in LDCL. However, rHu-IL-2 triggering became efficient when cells enriched in IL-2R-bearing macrophages were preincubated overnight with rHu-IL-2R. This response may point out a functional role for IL-2R and provide a role for IL-2 in certain macrophage functions.
Laboratorie d'Immunologie, Department de Biotechnologies, Roussel-Uclaf, Romainville, France.
B
B
Dugas
L
L
Friteau
H
H
Schellekens
D
D
Lando
C
C
Damais
D000367:Age Factors; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005455:Fluorescent Antibody Technique; D007371:Interferon-gamma / Q000494:pharmacology* / Q000502:physiology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis* / Q000187:drug effects; D011994:Recombinant Proteins
D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins; D007371:Interferon-gamma