Effect of 9,10-dimethyl-1,2-benzanthracene on the growth of hamster dermal fibroblasts in primary culture.
Newborn Syrian hamster dermal fibroblasts in primary culture have high plating efficiency, manifest as homogeneous contact-sensitive monolayer in mass cultures and above all possesses a short life-span of 15-20 days in culture. These cells are quite sensitive to mutagenic carcinogens. The DMBA treatment induced multi-layer and foci formation and growth in soft-agar suggesting that immortal variants can be obtained by treatment with carcinogens.
Carcinogenesis Division Cancer Research Institute, Tata Memorial Centre, Bombay, India.
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Rao
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Bhide
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Khadke
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Pendse
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D008647:Mesocricetus; D012867:Skin / Q000166:cytology*
D015127:9,10-Dimethyl-1,2-benzanthracene