Regulation and a possible stage-specific function of Oct-2 during pre-B-cell differentiation.
The Oct-2 gene appears to encode a developmental regulator of immunoglobulin gene transcription. We demonstrate that the Oct-2 gene is expressed at low levels in a variety of transformed pre-B-cell lines and is induced specifically in these cells by lipopolysaccharide signalling. This work extends an earlier observation in the pre-B-cell line 70Z/3 and therefore suggests that the inducible expression of the Oct-2 gene, like that of the kappa gene, is a characteristic feature of the pre-B stage of B-cell development. In 70Z/3 cells, the lymphokine interleukin-1 also induces the expression of the Oct-2 and kappa loci. Interestingly, expression of the Oct-2 gene is rapidly induced at the transcriptional level and may not require de novo protein synthesis. Since the changes in the activity of the Oct-2 locus completely correlate with the changes of the activity of the kappa locus, the two genes may be transcriptionally regulated by a common trans-acting factor. In 70Z/3 cells, transforming growth factor beta, an inhibitor of kappa-gene induction, blocks the upregulation of Oct-2 but not the activation of NF-kappa B. These results suggest that the combinatorial action of increased levels of Oct-2 and activated NF-kappa B may be necessary for the proper stage-specific expression of the kappa locus.
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
C L
CL
Miller
A L
AL
Feldhaus
J W
JW
Rooney
L D
LD
Rhodes
C H
CH
Sibley
H
H
Singh
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000502:physiology*; D015152:Blotting, Northern; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured; D004268:DNA-Binding Proteins; D005786:Gene Expression Regulation / Q000187:drug effects*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics / Q000502:physiology; D007375:Interleukin-1 / Q000494:pharmacology; D008070:Lipopolysaccharides; D051379:Mice; D050813:Octamer Transcription Factor-2; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D015533:Transcriptional Activation; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D004268:DNA-Binding Proteins; D007145:Immunoglobulin kappa-Chains; D007375:Interleukin-1; D008070:Lipopolysaccharides; D050813:Octamer Transcription Factor-2; C489206:Pou2f2 protein, mouse; D014157:Transcription Factors; D016212:Transforming Growth Factor beta