Concomitant synthesis and degradation of glutamine in isolated rabbit kidney tubules.
When rabbit kidney tubules were incubated with 1 mM [1-14C]glutamine as substrate, a release of 14CO2 together with a net production of glutamine were observed. That glutamine utilization was masked by higher rates of concomitant glutamine synthesis was demonstrated by: (i) inhibiting glutamine synthesis; and (ii) measuring the specific radioactivity of [1-14C]glutamine which fell during incubation.
Laboratoire de Physiologie Rénale et Métabolique, CNRS URA 1177 et INSERM U 80, Lyon, France.
S
S
Dugelay
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G
Baverel
D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D005260:Female; D005973:Glutamine / Q000096:biosynthesis* / Q000378:metabolism; D006868:Hydrolysis; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000378:metabolism; D007684:Kidney Tubules / Q000378:metabolism*; D011817:Rabbits
D005973:Glutamine; D002245:Carbon Dioxide