Extracellular materials in the endothelial meshwork of organcultured human trabecular meshwork. Morphologic and morphometric study.
Twenty-three normal cadaver eyes were used in this study. Eight trabecular meshwork explants were dissected at 45-degree intervals from a 77-year-old donor eye and cultured for 2 weeks for studying circumferential differences in the amount of extracellular materials in the endothelial meshwork. One trabecular meshwork explant was dissected from each of 22 individual eyes (age range: 64-89 years) and cultured for 2 weeks to study the effect of age on the amount of the extracellular materials. Then, 5 consecutive electron micrographs in the endothelial meshwork were obtained from the individual specimens and subjected to morphometric studies. In the endothelial meshwork, the results revealed: (1) no statistically significant circumferential differences in the amount of extracellular materials and (2) no statistically significant changes associated with aging in the amount of these materials.
Urakawa
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Y
Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto, Japan.
D002452:Cell Count; D016922:Cellular Senescence / Q000502:physiology; D004727:Endothelium / Q000166:cytology; D005109:Extracellular Matrix / Q000648:ultrastructure*; D006801:Humans; D009924:Organ Culture Techniques; D012016:Reference Values; D014129:Trabecular Meshwork / Q000166:cytology*