Calcium is required for the reduction of sulfite from hydrogen in a reconstituted electron transfer chain from the sulfate reducing bacterium, Desulfovibrio gigas.
Calcium is found a strong stimulator of sulfite reduction from hydrogen. A coupling protein of molecular weight 65,000 can be isolated from Desulfovibrio gigas. It functions in a reconstituted electron transfer chain between hydrogenase and sulfite reductase. Its N-terminal sequence shows high homologies with calcium or magnesium binding sites from other calcium-binding proteins.
Department of Biochemistry, University of Georgia, Athens 30602.
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D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification; D003901:Desulfovibrio / Q000378:metabolism*; D004579:Electron Transport; D004591:Electrophoresis, Polyacrylamide Gel; D006859:Hydrogen / Q000378:metabolism*; D006864:Hydrogenase / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013447:Sulfites / Q000378:metabolism*
D001426:Bacterial Proteins; D002147:Calmodulin; D002352:Carrier Proteins; D013447:Sulfites; D006859:Hydrogen; D006864:Hydrogenase; D050862:Oxidoreductases Acting on Sulfur Group Donors; D002118:Calcium