The usefulness of sequence analysis of amplified DNA for the identification of delta chain variants.
A method of identifying delta chain variants using relatively small volumes of blood is described. The procedure consists of the amplification of two segments of genomic DNA with two sets of delta chain specific primers and sequencing of the three exons (exons 1, 2, and 3) which are part of the amplified DNA segments. Data for three delta chain variants present in seven adult heterozygotes are presented.
Li
H W
HW
Department of Cell and Molecular Biology, Medical College of Georgia, Augusta 30912-2100.
Codrington
J F
JF
Schiliro
G
G
Wadsworth
L D
LD
Beris
P
P
Adekile
A
A
Huisman
T H
TH
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D044383:Black People / Q000235:genetics; D004252:DNA Mutational Analysis; D005006:Ethnicity; D005091:Exons; D005914:Globins / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D007585:Jews; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013789:Thalassemia / Q000150:complications / Q000235:genetics
D005914:Globins