Self peptide requirement for class II major histocompatibility complex allorecognition.
Using a dinitrophenylated and biotinylated peptide antigen, we have developed an affinity chromatography procedure to purify complexes of a given peptide species and a given class II major histocompatibility complex antigen away from class II molecules occupied by other peptides. We show that hen egg lysozyme peptide-I-Ed complexes purified according to this procedure have a greatly enhanced capacity to activate hen egg lysozyme-specific T cells but have lost the capacity to activate three different alloreactive T-cell hybridomas. These data demonstrate that the class II molecule in and of itself is not sufficient to activate alloreactive T cells. Rather, the data suggest that recognition of specific complexes formed between allo-class II and particular autologous peptides may be required. Alternatively, alloreactive T cells may be recognizing "empty" major histocompatibility complex molecules.
Demotz
S
S
Cytel, San Diego, CA 92121.
Sette
A
A
Sakaguchi
K
K
Buchner
R
R
Appella
E
E
Grey
H M
HM
D000595:Amino Acid Sequence; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D001710:Biotin / Q000737:chemistry; D002469:Cell Separation; D002846:Chromatography, Affinity; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006825:Hybridomas; D066298:In Vitro Techniques; D008024:Ligands; D008213:Lymphocyte Activation; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D009113:Muramidase / Q000276:immunology; D009211:Myoglobin / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D010455:Peptides / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D008024:Ligands; D046911:Macromolecular Substances; D009211:Myoglobin; D010455:Peptides; D001710:Biotin; D010047:Ovalbumin; D009113:Muramidase