Effect of induced acidosis on cytotoxicity of anticancer drugs.
The effect of adriamycin (ADR), vincristine (VCR), mitoxantrone (MTN) and cis-platin (PtCl) at acidic pH on the incorporation of 3H-thymidine (3H-TdR) was studied in vitro on P388 murine leukemia cells. The incorporation was inhibited at low pH by the four drugs used. In order to induce acidosis in tumor bearing mice, 6 g/kg glucose was administered intraperitoneally. The lowest pH that could be obtained was 6.8 two hours after glucose administration. The drugs ADR, VCR, MTN and PtCl administered to tumor-bearing mice 2 h following induction of acidosis resulted in significant increase in the life span of the tumor bearing mice.
Adwankar
M
M
Cellular Chemotherapy Unit, Tata Memorial Centre, Parel, Bombay, India.
Juvekar
A
A
Chitnis
M
M
D000138:Acidosis / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001202:Ascitic Fluid / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005260:Female; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007941:Leukemia P388 / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D005947:Glucose