Transformation of the yeast Saccharomyces kluyveri by Saccharomyces cerevisiae-based plasmids.
For the transformation of the yeast Saccharomyces kluyveri, ura3 mutants were obtained by 5-fluoro-orotic acid selection. By utilizing the method based on treatment of intact cells with alkali cations, the ura3 strains of S. kluyveri were transformed by Saccharomyces cerevisiae-based plasmids. In the transformed cells, a S. cerevisiae centromere-based plasmid was stably replicated autonomously. Thus, this system will permit the study of gene expression and its regulation in S. kluyveri in relationship to that in S. cerevisiae.
Fujimura
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Laboratory for Molecular Biology, Pharma Research Laboratories, Hoechst Japan Limited, Kawagoe.
D000667:Ampicillin / Q000494:pharmacology; D000668:Ampicillin Resistance / Q000235:genetics; D002503:Centromere / Q000502:physiology; D004587:Electrophoresis, Agar Gel; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D009154:Mutation / Q000235:genetics; D009963:Orotic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics*; D012440:Saccharomyces / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014170:Transformation, Genetic / Q000235:genetics*; D014498:Uracil / Q000494:pharmacology
D014498:Uracil; D009963:Orotic Acid; D000667:Ampicillin; C001242:5-fluoroorotic acid; D005020:Ethyl Methanesulfonate