Determination of a new blood glucose-lowering agent in biological fluids by capillary gas chromatography using electron-capture detection.
A gas chromatographic method for the determination of 5-(4-chlorophenyl)-2-[(4-methylphenyl)sulphonyl]-4-pentynoic acid (I) in plasma (serum) and urine has been developed. After an extraction process, the cleaned-up organic extract was derivatized with diazomethane at ambient temperature. Results are evaluated from peak-height ratios with respect to the appropriate internal standard. The detection limit following extraction of a 1-ml plasma sample is about 20 ng/ml.
Boehringer Mannheim GmbH, Bioanalytical Department, Germany.
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D002849:Chromatography, Gas; D004563:Electrochemistry; D005231:Fatty Acids, Unsaturated / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D007004:Hypoglycemic Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D007202:Indicators and Reagents; D012015:Reference Standards; D013450:Sulfones / Q000032:analysis* / Q000097:blood / Q000652:urine
D005231:Fatty Acids, Unsaturated; D007004:Hypoglycemic Agents; D007202:Indicators and Reagents; D013450:Sulfones; C071480:5-(4-chlorophenyl)-2-((4-methylphenyl)sulfonyl)-4-pentynoic acid