Whole blood re-calcification time in equine colic.
Whole blood re-calcification times were evaluated as a measure of endotoxin-associated coagulopathy in horses. First, the effects of endotoxin concentration and duration of in vitro incubation of citrated whole blood with endotoxin on the whole blood re-calcification time of blood collected from healthy horses were determined. Increasing concentrations or incubation times of endotoxin accelerated the whole blood re-calcification time. This effect was attributed mainly to increased monocyte thromboplastin activity. Second, whole blood re-calcification time, a clotting profile, plasma factor VII activity and plasma endotoxin concentration on blood samples obtained from 35 equine colic patients and 10 healthy horses were determined. Compared with healthy horses, colic patients had a longer mean whole blood re-calcification and prothrombin time, lower per cent factor VII activity and higher mean fibrin degradation products concentration. Within the colic patient group, horses that did not survive had detectable endotoxin in plasma, longer whole blood re-calcification and prothrombin times, and lower plasma factor VII activity, compared with colic patients that survived. These data indicate that colic patients with endotoxaemia experience hypercoagulable states, followed by consumptive coagulopathy. Although the cause of endotoxin-associated coagulopathy is likely multi-factorial, increased expression of monocyte thromboplastin activity may be involved in the pathogenesis of coagulopathy. The whole blood recalcification time is a simple, fast and inexpensive way to detect coagulopathy during endotoxaemia and determine the prognosis for survival.
Department of Large Animal Medicine, University of Georgia, College of Veterinary Medicine, Athens 30602.
M M
MM
Henry
J N
JN
Moore
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D003085:Colic / Q000097:blood / Q000662:veterinary*; D004731:Endotoxins / Q000097:blood / Q000494:pharmacology*; D004926:Escherichia coli; D005340:Fibrinogen / Q000032:analysis; D006734:Horse Diseases / Q000097:blood*; D006736:Horses; D010314:Partial Thromboplastin Time / Q000662:veterinary; D011517:Prothrombin Time / Q000662:veterinary; D013918:Thrombin Time / Q000662:veterinary
D004731:Endotoxins; C044101:endotoxin, Escherichia coli; D005340:Fibrinogen