Analysis of Aspergillus nidulans conidial antigens and their prevalence in other Aspergillus species.
Aspergillus nidulans is an ascomycetous fungus that reproduces asexually by forming multicellular conidiophores and uninucleate spores called conidia. These elements constitute the main vehicle for the transmission of this and other pathogenic Aspergillus species and are the starting point of the different forms of aspergillosis. In order to use A. nidulans as a potential source of useful antigens for the immunodiagnosis of these diseases, we have examined the total protein composition of conidial extracts of this fungus by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis in gels of different percent T. Injection of SDS-extracted conidial proteins into rabbits allowed us to raise a battery of polyclonal antibodies which have defined some important immunogenic polypeptides. Several of these immunogens were both present in mycelial extracts and recognized by antimycelium antibodies. Four of them, designated cdA, cdB, cdC, and cdE, were also found in conidial extracts of other pathogenic Aspergillus species. Only cdE was undetectable in cell extracts of the nonrelated species Fusarium culmorum and Phycomyces blakesleeanus.
Puente
P
P
Departamento de Microbiología y Genética and Instituto de Microbiología Bioquímica, Universidad de Salamanca/Consejo Superior de Investigaciones Científicas, Spain.
Ovejero
M C
MC
Fernández
N
N
Leal
F
F
D000946:Antigens, Fungal / Q000032:analysis*; D001230:Aspergillus / Q000276:immunology*; D001233:Aspergillus nidulans / Q000254:growth & development / Q000276:immunology*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis / Q000276:immunology; D007114:Immunization; D008970:Molecular Weight; D015995:Prevalence
D000946:Antigens, Fungal; D005656:Fungal Proteins