Use of Rambach Propylene Glycol Containing Agar for identification of Salmonella spp.
When grown on Rambach Propylene Glycol Containing Agar (Rambach agar), 216 of 230 (93.9%) Salmonella organisms isolated from patients and 54 of 62 (87.1%) Salmonella stock cultures produced a crimson-colored growth. Of the 14 clinical Salmonella isolates which displayed colors other than crimson, 8 were Salmonella typhi, 2 were Salmonella paratyphi A, and 4 belonged to other commonly isolated serotypes. All eight Salmonella stock cultures which failed to produce a crimson color belonged to rarely isolated serotypes. In contrast, of 83 non-Salmonella stock cultures distributed among 29 bacterial species, none produced a crimson color. These results suggest that while Rambach agar cannot preidentify S. typhi and S. paratyphi A, the medium can be used for the presumptive identification and can assist in the definitive identification of the overwhelming majority of Salmonella isolates.
Enteric Bacteriology Laboratory, New York City Department of Health, New York 10016.
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Gruenewald
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Henderson
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Yappow
D000362:Agar; D001431:Bacteriological Techniques; D005069:Evaluation Studies as Topic; D006801:Humans; D010858:Pigmentation; D019946:Propylene Glycol; D011409:Propylene Glycols; D012475:Salmonella / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D012703:Serotyping; D013045:Species Specificity
D011409:Propylene Glycols; D019946:Propylene Glycol; D000362:Agar