Isoforms of soybean seed oil body membrane protein 24 kDa oleosin are encoded by closely related cDNAs.
We have characterized two cDNA clones for 24 kDa soybean oleosin, the seed oil body membrane protein. Differences in the predicted amino acid sequences of the two clones and the presence of a doublet on immunoblots indicate that 24 kDa oleosin exists in at least two isoforms in soybean. The predicted amino acid sequence also contains a unique carboxy terminal region that is dominated by a series of different tandem amino acid repeats.
Plant Molecular Biology Laboratory, United States Department of Agriculture, Beltsville, MD 20705.
A
A
Kalinski
D S
DS
Loer
J M
JM
Weisemann
B F
BF
Matthews
E M
EM
Herman
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D013025:Glycine max / Q000235:genetics*
D008565:Membrane Proteins; D010940:Plant Proteins; C079299:oleosin 24 protein, Glycine max; D004247:DNA