Genetic and sequence analyses of a Pseudomonas denitrificans DNA fragment containing two cob genes.
A genetic analysis of a 12-kb DNA fragment containing Pseudomonas denitrificans cob genes was performed by transposon-mediated insertional mutagenesis. The nucleotide sequence and genetic analysis have shown that a 4.8-kb DNA subfragment carried two cob genes (cobS and cobT). Biochemical data concerning the complemented cobS and cobT mutants suggested that the cobS product was involved in cobalt insertion-mediating reactions and that the cobT product was involved in the transformation of precorrin-3 into cobyrinic acid.
Unité de Biologie Moléculaire, Institut des Biotechnologies, Virty-sur-Seine, France.
B
B
Cameron
C
C
Guilhot
F
F
Blanche
L
L
Cauchois
M C
MC
Rouyez
S
S
Rigault
S
S
Levy-Schil
J
J
Crouzet
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification*; D015247:Deoxyribonuclease HindIII; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D011549:Pseudomonas / Q000235:genetics*; D014805:Vitamin B 12 / Q000096:biosynthesis / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D015247:Deoxyribonuclease HindIII; D014805:Vitamin B 12