Assignment of two of the translation initiation factor-4E (EIF4EL1 and EIF4EL2) genes to human chromosomes 4 and 20.
The eukaryotic translation initiation factor (eIF-4E) has recently been cloned from human, mouse, and yeast. This polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. We have designed oligonucleotide primers to the 3' untranslated region of the gene encoding eIF-4E and specifically amplified the human gene in human/rodent somatic cell hybrids using the polymerase chain reaction. By this method, one of the human eIF-4E genes (EIF4EL1, eukaryotic translation initiation factor 4E-like 1) has been mapped to human chromosome 4qter-4p15. In addition, we have localized a second eIF-4E gene (EIF4EL2, eukaryotic translation initiation factor 4E-like 2) to human chromosome 20 by Southern blot analysis of mapping panels established from human/rodent somatic cell hybrids.
Department of Biology, Massachusetts, Institute of Technology, Cambridge 02139.
J
J
Pelletier
J D
JD
Brook
D E
DE
Housman
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002890:Chromosomes, Human, Pair 20; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular; D004247:DNA / Q000097:blood / Q000235:genetics; D004912:Erythrocytes / Q000502:physiology; D039561:Eukaryotic Initiation Factor-4E; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010448:Peptide Initiation Factors / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D039561:Eukaryotic Initiation Factor-4E; D010448:Peptide Initiation Factors; D004247:DNA