T-cell-dependent accumulation of eosinophils in the lung and its inhibition by monoclonal anti-interleukin-5.
The transnasal administration of an extract of the parasite Ascaris suum to C57BL/6 mice for 3 weeks produced marked eosinophilia in the bronchoalveolar lavage (BAL) fluid. The oral administration of ciclosporin significantly suppressed the pulmonary eosinophilia. Athymic C57BL/6-nu/nu mice failed to develop pulmonary eosinophilia. These data indicate that the pulmonary eosinophilia caused by this parasite extract is T-cell-dependent. Genetically mast-cell-deficient (WB x C57BL/6) F1-W/Wv (W/Wv) mice developed marked eosinophilia in the BAL, which shows that mast cells are not necessary in the formation of lung eosinophilia in this model. Monoclonal antimurine interleukin-5 injected intraperitoneally clearly inhibited the infiltration of eosinophils in the lung, suggesting that T-cell-derived interleukin-5 is essential.
Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Japan.
H
H
Okudaira
M
M
Nogami
G
G
Matsuzaki
M
M
Dohi
M
M
Suko
S
S
Kasuya
K
K
Takatsu
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015848:Interleukin-5 / Q000276:immunology / Q000502:physiology*; D008168:Lung / Q000473:pathology*; D008297:Male; D008407:Mast Cells / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D011657:Pulmonary Eosinophilia / Q000209:etiology* / Q000517:prevention & control; D013601:T-Lymphocytes / Q000502:physiology*
D000911:Antibodies, Monoclonal; D015848:Interleukin-5