Integrin alpha 2 beta 1 (VLA-2) mediates reorganization and contraction of collagen matrices by human cells.
The capacity of cells to organize and contract collagen fibrils is fundamental to processes as diverse as embryogenesis and wound healing. We analyzed different beta 1 integrins on diploid fibroblasts for their role in modifying the tertiary structure of collagen matrices. Using monoclonal antibodies that block the interaction of integrins with their ligands, evidence was obtained that alpha 2 beta 1 integrin is required for the contraction of a type I collagen matrix. Further supporting the role of alpha 2 beta 1, cell lines expressing minimal levels of this integrin uniformly failed to contract collagen matrices. In addition, transfection of a full-length alpha 2 cDNA into one such cell line led to enhanced cell surface expression of alpha 2 beta 1 and conferred the de novo capacity to contract collagen matrices.
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
J A
JA
Schiro
B M
BM
Chan
W T
WT
Roswit
P D
PD
Kassner
A P
AP
Pentland
M E
ME
Hemler
A Z
AZ
Eisen
T S
TS
Kupper
D000911:Antibodies, Monoclonal / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D005434:Flow Cytometry; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D007700:Kinetics; D011487:Protein Conformation; D016029:Receptors, Very Late Antigen / Q000378:metabolism*; D014162:Transfection / Q000235:genetics
D000911:Antibodies, Monoclonal; D016029:Receptors, Very Late Antigen; D003094:Collagen