Termination of transcription of ribosomal RNA in Saccharomyces cerevisiae.
We have attempted to determine the site of termination of transcription of ribosomal RNA in the yeast, Saccharomyces cerevisiae. While a quantitative description of the termination sites of RNA polymerase I is not possible using presently available methods, we conclude that transcription of most molecules continues through a large portion of the adjacent enhancer region. There are two potential termination sites within the enhancer, one of which is near the binding site of the DNA binding protein REBI. In addition there is an apparently fail-safe termination site approximately 950 nucleotides beyond the 3' end of 35S ribosomal precursor RNA. Processing at the end of 35S RNA influences the choice of downstream termination site. Conversely downstream sequences also influence the site of termination.
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461.
S P
SP
Johnson
J R
JR
Warner
D015152:Blotting, Northern; D004275:DNA, Ribosomal / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D015870:Gene Expression; D012318:RNA Polymerase I / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012331:RNA, Fungal / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013728:Terminator Regions, Genetic
D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012318:RNA Polymerase I