Toxicity and EROD-inducing potency of 24 polycyclic aromatic hydrocarbons (PAHs) in chick embryos.
The toxicities (embryolethality) of 24 polycyclic aromatic hydrocarbons (PAHs) were determined in chick embryos using a 72-h test. The substances, dissolved in peanut oil, were injected into the air sacs of eggs preincubated for 7 days. LD50 values were determined for the four most toxic of the 24 compounds. Benzo[k]fluoranthene proved to be the most potent, with an LD50 of 14 micrograms (56 nmol)/kg egg. Dibenz[a,h]anthracene, benz[a]anthracene and benzo[b]naphthol[2,3-d]thiophene were a few times less toxic [LD50 = 39 micrograms (140 nmol)/kg, 79 micrograms (349 nmol)/kg and 82 micrograms (350 nmol)/kg, respectively]. The LD50 of benzo[k]fluoranthene was only about 5 times higher than that previously found for the most potent coplanar polychlorinated biphenyl (PCB), 3,3',4,4',5-pentachlorobiphenyl [LD50 = 3.1 micrograms (9.4 nmol)/kg], in the same kind of test. The toxicities of 18 of the PAHs in this study have also been evaluated previously using a 2-week test in chick embryos. Dibenz[a,h]anthracene, which had not been studied earlier in the 2-week test, proved to be almost as toxic as previously found for benzo[k]fluoranthene in that test. Several of the PAHs studied induced EROD activity in chick embryos, and, in general, the most toxic PAHs were also the most potent inducers of EROD.(ABSTRACT TRUNCATED AT 250 WORDS)
Brunström
B
B
Department of Zoophysiology, Uppsala University, Sweden.
Broman
D
D
Näf
C
C
D000818:Animals; D002642:Chick Embryo; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000187:drug effects; D007928:Lethal Dose 50; D010088:Oxidoreductases / Q000096:biosynthesis*; D011083:Polycyclic Compounds / Q000378:metabolism / Q000633:toxicity*
D011083:Polycyclic Compounds; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D019363:Cytochrome P-450 CYP1A1