Paramyxovirus tropism dependent on host proteases activating the viral fusion glycoprotein.
An essential step in paramyxovirus fusion (F) glycoprotein biosynthesis is the posttranslational endoproteolytic cleavage of the inactive precursor glycoprotein Fo by host cell proteases. When the Fo possesses a pair or a cluster of basic residues at the cleavage site, cleavage is catalyzed by a ubiquitous protease(s) and the infection is consequently pantropic. When the site is monobasic with a single arginine, cleavage is allowed to occur only by the enzyme(s) expressed in limited tissue types and the infection is localized there. We have isolated from chick embryo an example of the latter type of endoprotease specific for the single arginine motif and demonstrate its identity with the clotting factor Xa. The ectopic expression of the FXa appeared to be the sole determinant for the viral tropism in chick embryo. The latter type of protease specific for a paired or multiple basic cleavage motif have neither been identified nor characterized extensively. We show here that this cleavage can be induced by the yeast KEX2 protease, a unique subtilisin-like serine protease, responsible for pro factor processing at the paired basic sites.
Nagai
Y
Y
Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Japan.
Inocencio
N M
NM
Gotoh
B
B
D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo; D010450:Endopeptidases / Q000378:metabolism*; D009980:Influenza A virus / Q000235:genetics* / Q000502:physiology; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010252:Paramyxoviridae / Q000235:genetics* / Q000502:physiology; D011499:Protein Processing, Post-Translational; D013379:Substrate Specificity; D014760:Viral Fusion Proteins / Q000235:genetics* / Q000378:metabolism
D046911:Macromolecular Substances; D014760:Viral Fusion Proteins; D010450:Endopeptidases