Subcellular localization of acidic and basic PR proteins in tobacco mosaic virus-infected tobacco.
Infection of Samsum NN tobacco with tobacco mosaic virus (TMV) results in the induction of the synthesis of acidic and basic isoforms of many pathogenesis-related (PR) proteins. By immunogold-electromicroscopy we have shown that PR proteins accumulate mainly in cells around the necrotic spots of TMV-induced lesions. The acidic chitinases, beta-(1,3)-glucanases and thaumatin-like proteins were found to accumulate in extracellular "pocket-like" vesicles while the basic chitinases were found in electron dense inclusion bodies in the vacuoles. These structures were not detectable in PR-containing leaves devoid of virus nor in healthy plants.
Dore
I
I
Gorlaeus Laboratories, Leiden University, The Netherlands.
Legrand
M
M
Cornelissen
B J
BJ
Bol
J F
JF
D000914:Antibodies, Viral / Q000276:immunology; D002688:Chitinases / Q000378:metabolism; D043326:Glucan 1,3-beta-Glucosidase; D016253:Microscopy, Immunoelectron; D010935:Plant Diseases; D010940:Plant Proteins / Q000378:metabolism*; D010947:Plants, Toxic; D013549:Sweetening Agents; D014026:Nicotiana / Q000382:microbiology*; D014027:Tobacco Mosaic Virus / Q000378:metabolism* / Q000648:ultrastructure; D001617:beta-Glucosidase / Q000378:metabolism
D000914:Antibodies, Viral; D010940:Plant Proteins; D013549:Sweetening Agents; C053376:pathogenesis-related proteins, plant; C003427:thaumatin protein, plant; D002688:Chitinases; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase