Screening of murine monoclonal antibodies against living schistosomula of Schistosoma mansoni by radioimmunoassay.
A radioimmunoassay was developed to screen supernatants of murine monoclonal antibodies against surface antigens of living schistosomula of Schistosoma mansoni. Of 196 clones screened, 10% bound schistosomula. Of these, 74% bound only schistosomula. The remaining molecules also reacted with soluble adult worm antigens and soluble egg antigens as determined by enzyme-linked immunosorbent assay. Immunoblot analysis demonstrated that monoclonal antibody 204-3E4 reacted with a 68 kDa protein, a glycoprotein that induces substantial resistance against S. mansoni infection. Recognition of an 18 kDa antigen by 204-3F1 antibody was stage-specific with the antigen being expressed in cercariae, 3- and 24-h-old parasites but not 4-day, lung stage or adult worms. Monoclonal antibody 204-4E3 reacted with purified S. mansoni paramyosin. These data indicate that radioimmunoassay using living schistosomula is a rapid alternative method to identify murine hybridomas that secrete antibodies which react with surface antigens of S. mansoni.
Program in Geographic Medicine, Brown University, Providence, RI.
P M
PM
Wiest
A V
AV
Wisnewski
J H
JH
Johnson
B
B
Ramirez
T F
TF
Kresina
G R
GR
Olds
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D005260:Female; D006825:Hybridomas; D051379:Mice; D008815:Mice, Inbred Strains; D011863:Radioimmunoassay; D012550:Schistosoma mansoni / Q000254:growth & development / Q000276:immunology*
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D000954:Antigens, Surface