Cytokine-activated human monocytes show differential cytotoxicity toward fresh and cultured Kaposi's sarcoma cells.
We have examined the ability of peripheral blood monocytes (PBMs) isolated from AIDS-related Kaposi's sarcoma (KS) patients or normal donors to kill (a) autologous KS tumor cells from skin biopsies of AIDS patients, (b) a tumorigenic cell line derived from a histologically verified AIDS-KS skin tumor, and (c) the WEHI-164 fibrosarcoma line. Unstimulated PBMs, and PBMs activated by IFNs, IL-2, or TNF-alpha, were tested for their ability to lyse 51Cr-labeled tumor targets. PBMs from both normal and KS patients, when activated by cytokines, showed enhanced cytolysis of both WEHI-164 and the KS cell line. PBMs from two of three AIDS patients lysed their autologous fresh KS tumor cells. These results indicate that PBMs from AIDS-KS patients can be induced by cytokines to elicit potent antitumor activity, including cytolysis of autologous KS tumor cells.
Cancer Research Institute, University of California, San Francisco 94143-0128.
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D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000276:immunology; D000818:Animals; D016207:Cytokines / Q000494:pharmacology*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D009000:Monocytes / Q000276:immunology*; D012514:Sarcoma, Kaposi / Q000150:complications / Q000276:immunology*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D016207:Cytokines; D016898:Interferon-alpha; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma