Exact cleavage site of Alzheimer amyloid precursor in neuronal PC-12 cells.
We have identified the secretory cleavage site in the Alzheimer amyloid precursor (APP) in a non-transfected neuronal cell line, using cyanogen bromide digests of APP purified from medium conditioned by PC-12 cells which were differentiated to a neuronal phenotype. The results obtained are most consistent with proteolysis of the Lys16-Leu17 bond in the beta amyloid peptide, followed by partial removal of Lys16 by a basic carboxypeptidase.
Department of Psychiatry, Mount Sinai Medical Center, New York, NY 10029.
J P
JP
Anderson
F S
FS
Esch
P S
PS
Keim
K
K
Sambamurti
I
I
Lieberburg
N K
NK
Robakis
D000544:Alzheimer Disease / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D002850:Chromatography, Gel; D009474:Neurons / Q000378:metabolism*; D016716:PC12 Cells
D016564:Amyloid beta-Protein Precursor