Morphological studies on the effect of L-leucine methyl ester on mouse peritoneal macrophages in vitro.
Mouse peritoneal macrophages (MO) were treated in culture with 5 mM L-leucine methyl ester (L-Leu-OMe), for 5, 10, 20, 40, and 60 min. The treatment resulted in rapid vacuolisation of the cytoplasm due to the dilatation and disruption of lysosomes. Autophagy caused by lysosomal enzymes destroyed most of the cytoplasmic organelles by 40 minutes after L-Leu-OMe treatment, but the cell membrane and nucleus were in many MOs resistant to the damage. 60 min after L-Leu-OMe treatment most of the MOs were killed. It is supposed that the disruption of the lysosomes is caused by formaldehyde produced by the hydrolysis of L-Leu-OMe.
First Institute of Pathology and Experimental Cancer Research, Semmelweis University Medical School, Budapest, Hungary.
B
B
Szende
K
K
Lapis
J
J
Timar
F
F
Antoni
I
I
Csuka
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002478:Cells, Cultured; D003593:Cytoplasm / Q000187:drug effects / Q000648:ultrastructure; D005557:Formaldehyde / Q000494:pharmacology; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D008264:Macrophages / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D015388:Organelles / Q000187:drug effects / Q000648:ultrastructure; D010529:Peritoneal Cavity / Q000166:cytology*; D013997:Time Factors
D005557:Formaldehyde; C030365:leucine methyl ester; D007930:Leucine