[Immunobiological characteristics of Mycoplasma hyopneumoniae antigens and the diagnosis of infection caused by this organism].
The paper presents the developed variants of enzyme immunoassay (EIA) using two M. hyopneumoniae (R-14 strain) antigens obtained by biomass freezing and thawing and two Tween-dissolved antigens by gel chromatography. To identify the cultures isolated, an indirect immunofluorescence reaction was proposed by employing agar blocks. The developed methods made it possible to identify 23 bovine-isolated cultures as M. hyopneumoniae antigens. The latter were detected by EIA in diagnostically significant titers in serum and colostrum samples from 2 swine-breeding farms. A negative result was obtained in the respiratory mycoplasmosis-free farms.
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AIa
D000822:Animal Husbandry; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D005455:Fluorescent Antibody Technique; D009177:Mycoplasma pneumoniae / Q000276:immunology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D013552:Swine / Q000254:growth & development*; D013553:Swine Diseases / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D014455:Ukraine
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial
Immunobiologicheskaia kharakteristika antigenov Mycoplasma hyopneumoniae i diagnostika vyzyvaemoĭ eiu infektsii.