Effects of lead on kinetics of 3H-dopamine uptake by rat brain synaptosomes.
The effects of lead on 3H-dopamine (3H-DA) uptake in rat brain synaptosomes were studied. Pb inhibited 3H-DA uptake in vitro and in vivo in a concentration-dependent manner. Altered pH versus 3H-DA uptake demonstrated comparable inhibition in buffered acidic, neutral, and alkaline pH ranges, and higher inhibition was observed in neutral pH. Kinetic studies of ATP activation of 3H-DA uptake indicated competitive inhibition by Pb. The inhibition of 3H-DA uptake followed the Na+ concentrations. The results indicate that the Pb inhibition of 3H-DA uptake is pH, ATP, and Na+ dependent.
Boykin
M J
MJ
Division of Natural Sciences, Selma University, Alabama 36701.
Chetty
C S
CS
Rajanna
B
B
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004298:Dopamine / Q000493:pharmacokinetics*; D006863:Hydrogen-Ion Concentration; D007274:Injections, Intraperitoneal; D007854:Lead / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D007854:Lead; D004298:Dopamine