The growth inhibition of Streptococcus mutans by 5'-nucleotidase inhibitors from Areca catechu L.
New 5'-nucleotidase inhibitors named NF-86I, NF-86II were recently isolated from the seeds of Areca catechu L. NF-86I and NF86II showed inhibitory effects on the growth of Streptococcus mutans MT8148(c) and Streptococcus mutans MT6715(g), respectively. In addition, these inhibitors could inhibit insoluble glucan formation from sucrose. NF-86I and NF-86II were found to be polyphenolic substances. Some polyphenols such as tannic acid bind non-specifically to proteins (tannic activity). The 5'-nucleotidase inhibitors that we isolated did not show any such activity. However, the growth inhibitory activity and the inhibitory effect on water-insoluble glucan production were equal to tannic acid. It is therefore considered that these inhibitors bind specifically to the bacterial cell surface. Our findings suggest that the 5'-nucleotidase inhibitors NF-86I and NF-86II may be useful anti-plaque preventing agents.
Central Laboratory, Nippon Flour Mills Co., Ltd., Kanagawa, Japan.
M
M
Iwamoto
K
K
Uchino
T
T
Toukairin
K
K
Kawaguchi
T
T
Tatebayashi
H
H
Ogawara
Y
Y
Tonosaki
D015720:5'-Nucleotidase / Q000037:antagonists & inhibitors*; D001114:Areca / Q000737:chemistry*; D005419:Flavonoids; D010636:Phenols / Q000494:pharmacology; D010946:Plants, Medicinal; D011108:Polymers / Q000494:pharmacology; D059808:Polyphenols; D013295:Streptococcus mutans / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*
D005419:Flavonoids; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D015720:5'-Nucleotidase