Clearance of influenza virus respiratory infection in mice lacking class I major histocompatibility complex-restricted CD8+ T cells.
Transgenic mice homozygous for a beta 2-microglobulin (beta 2-m) gene disruption and normal mice that had been treated with a CD8-specific mAb were infected intranasally with an H3N2 influenza A virus. Both groups of CD8T cell-deficient mice eliminated the virus from the infected respiratory tract. Potent CTL activity was detected in lung lavage populations taken from mice with intact CD8+ T cell function, with minimal levels of cytotoxicity being found for inflammatory cells obtained from the antibody-treated and beta 2-m mutant mice. We therefore conclude that cells infected with an influenza A virus can be cleared from the respiratory tract of mice lacking both functional class I major histocompatibility complex (MHC) glycoproteins and class I MHC-restricted, CD8+ effector T cells.
Department of Immunology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105.
M
M
Eichelberger
W
W
Allan
M
M
Zijlstra
R
R
Jaenisch
P C
PC
Doherty
D000818:Animals; D016827:CD8 Antigens / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D005260:Female; D005805:Genes, MHC Class I; D006580:Genetic Carrier Screening; D006720:Homozygote; D016130:Immunophenotyping; D009980:Influenza A virus / Q000276:immunology*; D008168:Lung / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology; D008212:Lymphocyte Depletion; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009976:Orthomyxoviridae Infections / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D001613:beta 2-Microglobulin / Q000235:genetics*
D016827:CD8 Antigens; D001613:beta 2-Microglobulin