An androgen-inducible expression system for Saccharomyces cerevisiae.
A novel controllable expression system for Saccharomyces cerevisiae has been developed. Expression of the gene encoding the human androgen receptor, from a strong yeast promoter, results in transactivation of a hybrid promoter carrying androgen-responsive sequences such that a target gene may be expressed in an androgen-dependent manner. By selection of an appropriate combination of androgen receptor level, target-gene copy number and concentration of the androgenic ligand, dihydrotestosterone, the expression level can be set within a 1400-fold range with no detectable effect on normal cell growth.
Department of Genetics, Glaxo Group Research Ltd., Greenford, Middlesex, U.K.
I J
IJ
Purvis
D
D
Chotai
C W
CW
Dykes
D B
DB
Lubahn
F S
FS
French
E M
EM
Wilson
A N
AN
Hobden
D000728:Androgens / Q000378:metabolism*; D015153:Blotting, Western; D005786:Gene Expression Regulation; D006801:Humans; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011944:Receptors, Androgen / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D015533:Transcriptional Activation
D000728:Androgens; D011944:Receptors, Androgen