Identification of a matrix-associated region 5' of an immunoglobulin heavy chain variable region gene.
In the accompanying report (C. F. Webb, C. Das, S. Eaton, K. Calame, and P. Tucker, Mol. Cell. Biol. 11:5197-5205, 1991), we characterize B-cell-specific protein-DNA interactions at -500 and -200 bp upstream of the mu immunoglobulin heavy chain promoter whose abundances were increased by interleukin-5 plus antigen. Because of the high A + T/G + C ratio of these sequences and the consistent findings by others that enhancer- and promoterlike regions are often located near matrix-associated regions, we asked whether these sequences might also be involved in binding to the nuclear matrix. Indeed, DNA fragments containing the -500 binding site were bound by nuclear matrix proteins. Furthermore, UV cross-linking studies showed that the DNA binding site for interleukin-5-plus-antigen-inducible proteins could also bind to proteins solubilized from the nuclear matrix. Nuclear matrix-associated sequences have also been demonstrated on either side of the intronic immunoglobulin heavy chain enhancer. Our data suggest a topological model by which interactions among proteins bound to the promoter and distal enhancer sequences might occur.
Webb
C F
CF
Department of Immunobiology, Oklahoma Medical Research Foundation, Oklahoma City 73104.
Das
C
C
Eneff
K L
KL
Tucker
P W
PW
D000818:Animals; D000941:Antigens / Q000276:immunology; D034961:Antigens, Nuclear; D001483:Base Sequence; D004742:Enhancer Elements, Genetic / Q000502:physiology*; D005803:Genes, Immunoglobulin; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007148:Immunoglobulin mu-Chains / Q000235:genetics; D015848:Interleukin-5 / Q000494:pharmacology; D051379:Mice; D008954:Models, Biological; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000502:physiology*; D014407:Tumor Cells, Cultured
D000941:Antigens; D034961:Antigens, Nuclear; D007135:Immunoglobulin Variable Region; D007148:Immunoglobulin mu-Chains; D015848:Interleukin-5; D009687:Nuclear Proteins