Quantitation of histamine by planimetry of latex agglutination-inhibition results.
A new immunologic method to measure histamine concentration in blood is described. The method is based on the planimetric measurement of the agglutinated and precipitated area of heavy latex particles on a microtiter plate. The sensitivity of the assay was 0.1 ng/ml (= 1 nM) for histamine, and the coefficients of variation were 0.43-8.8% at 7 different concentrations (n = 4). No cross-reactivity occurred with histidine, 1-methyl histamine or norepinephrine. A good correlation with the radioimmunoassay was obtained for rabbit blood, in which histamine was released either by immunogen stimulation or injection of compound 48/80 (r = 0.977, n = 26). The procedures involved in this assay method are simple and can be completed within 4-6 hours. These results indicate the usefulness of the method for forensic medical tests and/or other clinical medical tests.
Kimura
H
H
Department of Forensic Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Matsuzawa
S
S
D000818:Animals; D005554:Forensic Medicine; D006632:Histamine / Q000097:blood*; D007841:Latex Fixation Tests; D011817:Rabbits
D006632:Histamine