Characterization of antibody response to Pseudomonas aeruginosa in patients with wound infections.
ELISA was used to measure the amount and avidity of IgG antibodies to exotoxin A (ExA) and 7 Fisher's immunotypes of lipopolysaccharide (LPS) in the sera of 13 patients with mild or moderate Pseudomonas aeruginosa infections. Changes in the specificity of tested sera during the course of infection were demonstrated. A statistically significant increase was seen in the amount and avidity of the antibodies to ExA in a majority of the sera, and an increase was seen in amount of antibodies to LPS immunotype 4 in the sera of patients with moderate infections.
Department of Microbiology, Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
E A
EA
Trafny
J
J
Grzybowski
J
J
Patzer
D
D
Popiel
J
J
Wrembel-Wargocka
D
D
Dzierzanowska
I
I
Zawistowska-Marciniak
K
K
Kobus
D036002:ADP Ribose Transferases; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000032:analysis*; D000915:Antibody Affinity / Q000276:immunology; D001427:Bacterial Toxins; D004797:Enzyme-Linked Immunosorbent Assay; D005098:Exotoxins / Q000032:analysis; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D016130:Immunophenotyping; D008070:Lipopolysaccharides / Q000032:analysis; D008297:Male; D008875:Middle Aged; D011552:Pseudomonas Infections / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology* / Q000302:isolation & purification; D037521:Virulence Factors; D014946:Wound Infection / Q000276:immunology* / Q000382:microbiology; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D005098:Exotoxins; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A