Evaluation of a direct fluorescent antibody test for detection of Chlamydia trachomatis in endocervical specimens. Brief report.
A total of 160 endocervical specimens collected from 72 symptomatic and 82 asymptomatic women attending a gynecology outpatient clinic were investigated for genital Chlamydia trachomatis infection by the use of tissue culture and DFA test. The infection rate was 42% for symptomatic and 23% for asymptomatic patient groups. The sensitivity rates of the DFA test in the symptomatic and asymptomatic groups were 84% and 75%, while the specificity rates were 89% and 95%, respectively. The DFA test had an overall sensitivity of 80% and specificity of 93%. There was 90% agreement between the two techniques. Therefore, DFA is recommended as an alternative to tissue culture where laboratory facilities are limited and genital chlamydial infections are highly prevalent.
Dereli
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D
Ege University Medical Faculty, Clinical Bacteriology and Infectious Diseases Department, Bornova-Izmir, Turkey.
Ertem
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E
Serter
D
D
Yüce
K
K
D000293:Adolescent; D000328:Adult; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D046508:Culture Techniques; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008875:Middle Aged; D012680:Sensitivity and Specificity; D002577:Uterine Cervical Diseases / Q000382:microbiology; D014626:Vaginal Smears