A novel assay for the in vivo study of Schwann cells.
An in vivo assay was developed for long-term analyses of Schwann cells at the single cell level. Schwann cells were isolated from sciatic nerves and labeled with the fluorescent gold label Fluoro-Gold. Cells were then transplanted into severed sciatic nerves of young adult rats. Gold-labeled Schwann cells, identified by double-labeling with S100 antibodies, were observed up to 10 mm away from the transplant site and after 90 and 120 days of survival in vivo.
Louisiana State University School of Veterinary Medicine, Department of Veterinary Anatomy and Fine Structure, Baton Rouge 70803.
J K
JK
Daniloff
D000818:Animals; D000906:Antibodies; D002465:Cell Movement; D002469:Cell Separation; D002470:Cell Survival; D003584:Cytological Techniques; D005456:Fluorescent Dyes; D009418:S100 Proteins / Q000276:immunology; D012583:Schwann Cells / Q000502:physiology* / Q000637:transplantation; D012584:Sciatic Nerve / Q000166:cytology / Q000502:physiology; D013266:Stilbamidines; D013997:Time Factors
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D000906:Antibodies; D005456:Fluorescent Dyes; D009418:S100 Proteins; D013266:Stilbamidines