[Features of transformation of competent cells and Bacillus subtilis protoplasts by integrative vectors].
The effect of structural peculiarities of DNAs from integrative plasmids on the transformation activity was studied. Monomeric forms of the plasmids can only transform B. subtilis competent cells, when plasmid selective marker is inserted into chromosomal fragment within the plasmid. Polymeric forms are needed for efficient transformation. Both single- and double-stranded DNAs of integrative plasmids transform no B.subtilis protoplasts, this being irrespective of plasmid structure.
V Z
VZ
Nezametdinova
A A
AA
Prozorov
D001412:Bacillus subtilis / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics; D002876:Chromosomes, Bacterial; D004279:DNA, Viral / Q000235:genetics; D005798:Genes, Bacterial; D005814:Genes, Viral; D005822:Genetic Vectors; D011523:Protoplasts; D012038:Regeneration; D012689:Sequence Homology, Nucleic Acid; D014170:Transformation, Genetic
D004279:DNA, Viral
Izuchenie osobennosteĭ transformatsii integrativnym vektorami kompetetnykh kletok i protoplastov Bacillus subtilis.