Functional analysis of the tobacco mosaic virus tRNA-like structure in cytoplasmic gene regulation.
The 3'-untranslated region (UTR) of tobacco mosaic virus (TMV), which terminates in a tRNA-like structure, functionally substitutes for a poly(A) tail in both plant and animal cells. The addition of the TMV 3'-UTR to chimeric mRNA constructs increases their expression up to 100-fold, increasing both translational efficiency and mRNA stability. The domain largely responsible for the regulation maps to a 72 base region immediately upstream of the tRNA-like structure, however, the 3'-terminal, tRNA-like structure is required for full function. Its contribution is lost if separated from the upstream pseudoknot domain by as few as 5 bases or if 6 bases are removed from the 3'-terminus. Sequence addition to the 3'-terminus of the TMV 3'UTR or the upstream pseudoknot domain inhibits function in both tobacco and Chinese hamster ovary cells.
Department of Biological Sciences, Stanford University, CA 94305-5020.
D R
DR
Gallie
J N
JN
Feder
R T
RT
Schimke
V
V
Walbot
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D006224:Cricetinae; D003593:Cytoplasm / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010944:Plants / Q000382:microbiology; D011061:Poly A; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D012343:RNA, Transfer / Q000737:chemistry* / Q000235:genetics; D013329:Structure-Activity Relationship; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014158:Transcription, Genetic
D012333:RNA, Messenger; D011061:Poly A; D012343:RNA, Transfer; D015252:Deoxyribonucleases, Type II Site-Specific; C038485:GGGCCC-specific type II deoxyribonucleases