An interleukin-1 inhibitor in gingival crevicular fluid of patients with chronic inflammatory periodontal disease.
The gingival crevicular fluid of a patient(s) with marginal periodontal disease contained an activity inhibitory to interleukin-1 (IL-1). The inhibitory activity could be detected after the depletion of IL-1 alpha by the use of a specific antibody (anti-human recombinant IL-1 alpha monoclonal antibody)-conjugated Sepharose column. The inhibitory activity was not due to a toxic effect on the thymocytes since IL-1 alpha-depleted gingival crevicular fluid did not affect the incorporation of [3H]thymidine in either the presence or absence of concanavalin A. The inhibitory activity was exerted against both IL-1 alpha and IL-1 beta. The inhibitory factor did not have any effect on IL-2-induced proliferation of concanavalin A-activated spleen cells. The inhibitor was heat labile. Gel filtration on a Superose 12 column revealed the IL-1 inhibitor to have two major peaks, one in the molecular size range of 12 to 14 kDa and the other below a molecular size of 10 kDa.
Kabashima
H
H
Department of Periodontics and Endodontics, Faculty of Dentistry, Kyushu University, Fukuoka, Japan.
Maeda
K
K
Iribe
H
H
Yamashita
K
K
Hirofuji
T
T
Iwamoto
Y
Y
Aono
M
M
D000818:Animals; D001681:Biological Assay; D002908:Chronic Disease; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000503:physiopathology; D007375:Interleukin-1 / Q000037:antagonists & inhibitors*; D008213:Lymphocyte Activation; D051379:Mice; D010518:Periodontitis / Q000503:physiopathology*
D007375:Interleukin-1