Phosphorylation of K+ channels in the squid giant axon. A mechanistic analysis.
Protein phosphorylation is an important mechanism in the modulation of voltage-dependent ionic channels. In squid giant axons, the potassium delayed rectifier channel is modulated by an ATP-mediated phosphorylation mechanism, producing important changes in amplitude and kinetics of the outward current. The characteristics and biophysical basis for the phosphorylation effects have been extensively studied in this preparation using macroscopic, single-channel and gating current experiments. Phosphorylation produces a shift in the voltage dependence of all voltage-dependent parameters including open probability, slow inactivation, first latency, and gating charge transferred. The locus of the effect seems to be located in a fast 20 pS channel, with characteristics of delayed rectifier, but at least another channel is phosphorylated under our experimental conditions. These results are interpreted quantitatively with a mechanistic model that explains all the data. In this model the shift in voltage dependence is produced by electrostatic interactions between the transferred phosphate and the voltage sensor of the channel.
Perozo
E
E
Department of Physiology, Jerry Lewis Neuromuscular Research Center, Los Angeles, California.
Bezanilla
F
F
D000818:Animals; D001369:Axons / Q000378:metabolism*; D049832:Decapodiformes; D010766:Phosphorylation; D015221:Potassium Channels / Q000378:metabolism*
D015221:Potassium Channels