A metallothionein-like gene from maize (Zea mays). Cloning and characterization.
A differentially expressed maize gene has been cloned and sequenced. Transcriptional and translational start sites have been mapped and 2.5 kb of 5' flanking DNA were sequenced. The 8 kDa protein encoded by this gene shows striking similarity to the metallothionein-like proteins recently described in Pisum sativum and Mimulus guttatus. The maize MT-L gene message is very abundant in roots without exposure to high levels of metals, present at lower concentration in leaves and pith, and at very low concentration in seed.
de Framond
A J
AJ
Department of Plant Molecular Biology, CIBA-Geigy Agricultural Biotechnology Research Unit, Research Triangle Park, NC 27709-2257.
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D008668:Metallothionein / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D014158:Transcription, Genetic; D003313:Zea mays / Q000235:genetics*
D012333:RNA, Messenger; D004247:DNA; D008668:Metallothionein