Identification of 4-chlorobenzoyl-coenzyme A as intermediate in the dehalogenation catalyzed by 4-chlorobenzoate dehalogenase from Pseudomonas sp. CBS3.
The intermediate in the reaction catalyzed by 4-chlorobenzoate dehalogenase from Pseudomonas sp. CBS3 was identified as 4-chlorobenzoyl-CoA. One component of 4-chlorobenzoate dehalogenase worked as a a 4-chlorobenzoyl-CoA ligase catalyzing the formation of 4-chlorobenzoyl-CoA from 4-chlorobenzoate, coenzyme A and ATP. This intermediate was detected spectrophotometrically and by HPLC. 4-chlorobenzoyl-CoA was the substrate for the dehalogenase component, which catalyzed the conversion to 4-hydroxybenzoate with concomitant release of coenzyme A.
Löffler
F
F
Technische Universität Hamburg-Harburg, Arbeitsbereich Biotechnologie II, Germany.
Müller
R
R
D000255:Adenosine Triphosphate / Q000378:metabolism; D002723:Chlorobenzoates / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003065:Coenzyme A / Q000378:metabolism*; D006867:Hydrolases / Q000378:metabolism*; D008025:Ligases / Q000378:metabolism; D011549:Pseudomonas / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D002723:Chlorobenzoates; D000255:Adenosine Triphosphate; D006867:Hydrolases; C042846:4-chlorobenzoate dehalogenase; D008025:Ligases; C040768:4-chlorobenzoic acid; D003065:Coenzyme A