High-performance liquid chromatographic analysis of trilostane and ketotrilostane in rat plasma.
A simple high-performance liquid chromatographic (HPLC) method for assaying trilostane, a synthetic steroid, and one of its metabolites, ketotrilostane, in small volumes of rat plasma has been developed. A single liquid-liquid extraction was used to isolate the two compounds from acidified plasma prior to the quantitative analysis. The HPLC conditions involved the use of a Spherisorb ODS column (250 mm x 4.6 mm I.D.) and a mobile phase of 1,4-dioxan-Sorenson's buffer at pH 5.0 (52:48, v/v). Ethisterone was used as an internal standard. Trilostane and ketotrilostane were detected by their ultraviolet absorbance at 255 nm. Recoveries greater than 80% and detection limits of 50 ng/ml were obtained for both compounds. Inter-day coefficients of variation were less than 10%.
Department of Pharmaceutical Sciences, University of Nottingham, University Park, U.K.
J P
JP
McGee
K J
KJ
Palin
P N
PN
Shaw
C
C
Potter
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D013196:Dihydrotestosterone / Q000031:analogs & derivatives* / Q000097:blood; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D013196:Dihydrotestosterone; C044207:17-ketotrilostane; C009954:trilostane