Mutants of initiator tRNA that function both as initiators and elongators.
We describe the effect of mutations in the acceptor stem of Escherichia coli initiator tRNA on its function in vivo. The acceptor stem mutations were coupled to mutations in the anticodon sequence from CAU----CUA to allow functional studies on the mutant tRNAs in initiation and in elongation in vivo. We show that, with one exception, there is a good correlation between the kinetic parameters for formylation of the mutant tRNAs in vitro (preceding paper, Lee, C.P., Seong, B. L., and RajBhandary, U.L. (1991) J. Biol. Chem. 266, 18012-18017) and their activity in initiation in vivo. These results suggest an important role for formylation of initiator tRNA in its function in initiation, at least when it is aminoacylated with glutamine as is the case with the mutant tRNAs used here. Mutant tRNAs that have a base pair between nucleotides 1 and 72 at the top of the acceptor stem function as elongators, as analyzed by their ability to suppress an amber mutation in the E. coli beta-galactosidase gene. One of these mutants is also quite active in initiation. Thus, activities of a tRNA in initiation and elongation steps of protein synthesis are not mutually exclusive. Using a mRNA with two in frame UAG codons, we show that this mutant tRNA can both initiate protein synthesis from the upstream UAG and suppress the down-stream UAG. We discuss the potential use of tRNAs with such "dual" functions in tightly regulated expression of genes for proteins in E. coli.
Varshney
U
U
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Lee
C P
CP
Seong
B L
BL
RajBhandary
U L
UL
D000667:Ampicillin / Q000494:pharmacology; D000926:Anticodon; D001483:Base Sequence; D015153:Blotting, Western; D002701:Chloramphenicol / Q000494:pharmacology; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D007612:Kanamycin / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D012343:RNA, Transfer / Q000235:genetics*; D001618:beta-Lactamases / Q000378:metabolism
D000926:Anticodon; D007612:Kanamycin; D002701:Chloramphenicol; D000667:Ampicillin; D012343:RNA, Transfer; D015500:Chloramphenicol O-Acetyltransferase; D001618:beta-Lactamases