[An experimental models in the evaluation of the effect of the amniotic fluid].
Fetal life develops in a fluid environment. Amniotic fluid is not inert. It contains proteins, hormones, fetal urine and electrolytes, known to interact with a variety of fetal processes such as wound healing, intestinal growth, etc. Interest in its study has been increasing recently. In order to determine the effect of amniotic fluid, intra-amniotic and extra-amniotic fetuses should be compared. We describe a model that allows isolation of a fetal portion from amniotic fluid. Sixty 23-day pregnant rabbits were operated on. Surgery was performed in two fetuses of each mother. One fetus was replaced into the amniotic sac; the other was sutured along its waist to the histerotomy orifice, leaving the caudal half of the fetus in an extra-amniotic position. The mothers continued their pregnancy and were sacrificed on day 30. Maternal survivance rate was 82 per 100. Twelve intra-amniotic fetuses (FIA) and nine extra-amniotic fetuses (FEA) were obtained, as well as 16 nonoperated littermates that served as controls. The average weight of FIA fetuses was 31 g, FEA fetuses weighed 37 g and controls 36 g. The difference was not statistically significant between any of the groups. This model allows the study of a variety of fetal conditions in the absence of amniotic fluid. The survivance rate of this technique of extrauterine fixation has been 75 per 100.
Servicio de Cirugía Pediátrica, Hospital Clínico, Barcelona.
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A
Albert
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Julia
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Morales
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F
Díaz
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MT
Peña
D000653:Amniotic Fluid / Q000502:physiology*; D000818:Animals; D005260:Female; D005313:Fetal Death / Q000209:etiology; D005333:Fetus / Q000601:surgery*; D011247:Pregnancy; D011817:Rabbits
Un modelo experimental para la valoracion del efecto del liquido amniotico.