A new in vitro model of Entamoeba histolytica adhesion, using the human colon carcinoma cell line Caco-2: scanning electron microscopic study.
The human colon carcinoma cell line Caco-2, which is widely used to study the adhesion and cytotoxicity of enterobacteria, was used to investigate the adhesion of the trophozoites of Entamoeba histolytica. We observed a high percentage of adhesion of amoebae to Caco-2 cells. Scanning electron microscopy showed that amoebial membrane structures were involved in adhesion and the cytolytic action. These differentiated cells should prove to be a useful model system for investigation of the pathogenic action of amoebae.
Rigothier
M C
MC
Laboratoire de Biologie et Contrôle des Organismes Parasites, Université de Paris XI, Châtenay-Malabry, France.
Coconnier
M H
MH
Servin
A L
AL
Gayral
P
P
D000818:Animals; D002448:Cell Adhesion; D003110:Colonic Neoplasms / Q000473:pathology; D004404:Dysentery, Amebic / Q000473:pathology*; D004748:Entamoeba histolytica / Q000472:pathogenicity*; D006801:Humans; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000469:parasitology*; D008855:Microscopy, Electron, Scanning; D014407:Tumor Cells, Cultured