In vitro type II binding of chromosomal DNA to membrane in Bacillus subtilis.
DNA-membrane association critical for initiation of DNA replication in Bacillus subtilis can be classified into two types. Type I is salt resistant and dependent on the initiation gene, dnaB, and type II is salt sensitive and independent of the dnaB gene. We found and sequenced two adjacent areas of type II binding within 1% of oriC on the B. subtilis chromosome.
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309-0347.
Y
Y
Sato
M
M
McCollum
T
T
McKenzie
J
J
Laffan
A
A
Zuberi
N
N
Sueoka
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D001665:Binding Sites; D002462:Cell Membrane / Q000502:physiology*; D002876:Chromosomes, Bacterial / Q000502:physiology*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics* / Q000502:physiology; D007700:Kinetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009994:Osmolar Concentration; D010957:Plasmids; D015183:Restriction Mapping
D004269:DNA, Bacterial