Induction of invasive carcinomas of the seminal vesicles and coagulating glands of F344 rats by administration of N-methylnitrosourea or N-nitrosobis(2-oxopropyl)amine and followed by testosterone propionate with or without high-fat diet.
The potential modifying effects of testosterone propionate (TP) and high-caloric high-fat diet (20% corn oil, HF) on rat accessory sex gland carcinogenesis were investigated. Male F344 rats were treated five times at 4-week intervals with N-methylnitrosourea (MNU) i.v. or N-nitrosobis(2-oxopropyl)amine (BOP) s.c., each injection following 2 weeks pretreatment with dietary ethinyl estradiol. After completion of this carcinogen administration stage, animal groups received subcutaneous implantation of Silastic tubes filled with 40 mg TP with or without HF for 40 weeks. Carcinomas of the seminal vesicles and/or coagulating glands were induced in 5, 39 and 56% of rats given MNU alone, MNU and TP, and MNU and HF plus TP respectively. No equivalent tumors were found in rats given MNU and HF. In the BOP-treated groups, 11% of animals receiving TP but no HF diet demonstrated seminal vesicle carcinomas and 6% of rats receiving TP plus HF diet had coagulating gland carcinoma. Thus while TP exerted a strong enhancing effect on tumor growth in the seminal vesicles and coagulating glands, high caloric HF did not manifest any significant influence.
First Department of Pathology, Nagoya City University Medical School, Japan.
T
T
Shirai
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A
Yamamoto
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S
Iwasaki
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S
Tamano
T
T
Masui
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002030:Bulbourethral Glands / Q000187:drug effects* / Q000469:parasitology; D003110:Colonic Neoplasms / Q000139:chemically induced; D004041:Dietary Fats / Q000009:adverse effects*; D004357:Drug Synergism; D005834:Genital Neoplasms, Male / Q000139:chemically induced*; D007680:Kidney Neoplasms / Q000139:chemically induced; D008175:Lung Neoplasms / Q000139:chemically induced; D008297:Male; D008770:Methylnitrosourea; D009602:Nitrosamines; D009929:Organ Size; D011467:Prostate / Q000033:anatomy & histology; D011471:Prostatic Neoplasms / Q000139:chemically induced; D011863:Radioimmunoassay; D051381:Rats; D011916:Rats, Inbred F344; D012669:Seminal Vesicles / Q000187:drug effects* / Q000473:pathology; D013739:Testosterone / Q000009:adverse effects* / Q000097:blood; D014523:Urethral Neoplasms / Q000139:chemically induced; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced
D004041:Dietary Fats; D009602:Nitrosamines; D013739:Testosterone; C013622:nitrosobis(2-oxopropyl)amine; D008770:Methylnitrosourea