Effects of interleukin 7 on the growth of clonogenic cells in T-cell acute lymphoblastic leukaemia.
The effects of the recombinant human cytokines interleukin 2 (IL-2) and IL-7 on the proliferation of T-acute lymphoblastic leukaemia (T-ALL) cells were tested in a clonogenic assay. Highly purified leukaemic cells were obtained by immunomagnetic depletion of mature T cells and fluorescence-activated cell sorting for immature leucocyte markers (CD1, CD10, CD34). Of 9 cases tested, only 3 showed evidence of stimulation by cytokines. One was stimulated by both IL-2 and IL-7, one by IL-2 only, and the third by IL-7 alone. A further case showed proliferation without addition of cytokines. The remaining 5 cases were completely unresponsive. While both IL-2 and IL-7 are capable of stimulating leukaemic cells from some cases of T-ALL, the molecules regulating the proliferation of T-ALL cells in vitro remain to be more fully elucidated.
Department of Haematology, Westmead Hospital, New South Wales, Australia.
V
V
Makrynikola
A
A
Kabral
K
K
Bradstock
D000293:Adolescent; D000328:Adult; D002455:Cell Division; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D015851:Interleukin-7 / Q000494:pharmacology*; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000473:pathology*; D008212:Lymphocyte Depletion; D008297:Male; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000473:pathology; D014410:Tumor Stem Cell Assay
D007376:Interleukin-2; D015851:Interleukin-7; D011994:Recombinant Proteins