In vitro translation in a hamster brain cell-free system.
We have developed a cell-free translation system derived from hamster brain tissue. The optimal incorporation conditions were 160 mM K+, 2 mM Mg2+ and microM spermine. The absence of this latter compound could be compensated only by doubling (to 4 mM) Mg2+ concentration. This system was inhibited by cycloheximide and emetine (elongation inhibitors) as well as NaF and aurintricarboxilic acid (ATA) (initiation inhibitors). The nature of the ATA inhibiting effect was studied by kinetic comparison with cycloheximide and polyribosome sedimentation pattern.
Thériaul
G R
GR
Département de Chimie et Biochimie, Université de Moncton, Canada.
Gauthier
D
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D000255:Adenosine Triphosphate / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001312:Aurintricarboxylic Acid / Q000494:pharmacology; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002474:Cell-Free System; D006224:Cricetinae; D004640:Emetine / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000378:metabolism; D066298:In Vitro Techniques; D008274:Magnesium / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D014176:Protein Biosynthesis; D012969:Sodium Fluoride / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology
D000596:Amino Acids; D013096:Spermine; D001312:Aurintricarboxylic Acid; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D012969:Sodium Fluoride; D008274:Magnesium; D011188:Potassium; D004640:Emetine