A class I jumping clone places the HLA-G gene approximately 100 kilobases from HLA-H within the HLA-A subregion of the human MHC.
By the combination of cosmid cloning, chromosomal jumping, and pulsed-field gel electrophoresis (PFGE), we have fine-mapped the HLA-A subregion of the human major histocompatibility complex (MHC). Through the isolation of a class I jumping clone, the Q alpha-like HLA-G class I gene has been placed within 100 kb of HLA-H. The tight physical linkage of these class I genes has been further supported by hybridizing PFGE blots with locus-specific probes. It has been found that both of the above class I genes are linked to HLA-A, with HLA-H residing no more than 200 kb from the HLA-A gene. These data support the possible existence of a Q alpha-like subregion composed of nonclassical HLA class I genes within the human MHC linked telomerically to the HLA-A locus.
Department of Human Genetics, Yale University School of Medicine, New Haven, Connecticut 06510.
H
H
Shukla
G A
GA
Gillespie
R
R
Srivastava
F
F
Collins
M J
MJ
Chorney
D002460:Cell Line; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D003001:Cloning, Molecular; D003360:Cosmids; D015723:Gene Library; D005805:Genes, MHC Class I; D006680:HLA Antigens / Q000235:genetics*; D015234:HLA-A Antigens / Q000235:genetics*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D008285:Major Histocompatibility Complex; D015183:Restriction Mapping
D006680:HLA Antigens; D015234:HLA-A Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I