Involvement of OP18 in cell proliferation.
Op18 is a highly conserved major cytosolic phosphoprotein that is expressed at high levels in acute leukemia and in neuroblastoma. In this study we present evidence pointing to a role for Op18 in cellular proliferation. Blocking of Op18 mRNA translation using antisense oligonucleotides delayed entrance of mitotically stimulated normal peripheral blood lymphocytes into the S phase. Moreover treatment of HL-60 promyelocytic leukemia cells with DMSO or PMA which induced terminal differentiation resulted in a decrease in the level of Op18 RNA and protein. Inhibition of lymphoid proliferation with cyclosporin also resulted in reduced Op18 levels.
Department of Pediatrics, University of Michigan Medical School, Ann Arbor 48109-0510.
R F
RF
Melhem
J R
JR
Strahler
N
N
Hailat
X X
XX
Zhu
S M
SM
Hanash
D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008868:Microtubule Proteins; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000502:physiology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010750:Phosphoproteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics; D050777:Stathmin; D013755:Tetradecanoylphorbol Acetate; D014407:Tumor Cells, Cultured
D008868:Microtubule Proteins; D009363:Neoplasm Proteins; D016376:Oligonucleotides, Antisense; D010750:Phosphoproteins; D012333:RNA, Messenger; C493995:STMN1 protein, human; D050777:Stathmin; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide