Plaque formation of Newcastle disease virus in primary chicken kidney cells.
Using primary chicken kidney (PCK) cells, a plaque assay was carried out with an avirulent strain of Newcastle disease virus (NDV) without adding trypsin to the agar overlay medium. The plaque assay was done in less than 4 days in PCK cells, by 3 days earlier than in primary chicken embryo (CE) cells maintained in the presence of trypsin. The curves of the progeny virus production began to rise 6 h after the infection and reached a plateau at 12 h. Equal amounts of virus were produced in PCK cells both in the presence and absence of trypsin in the culture medium. Viral peptide analysis revealed that a large portion of the HN and F precursor envelope glycoproteins of the NDV-Ulster strain remained uncleaved in PCK-grown virions. This suggests that a marginal proteolytic cleavage of these glycoprotein suffices for the full growth of the progeny virus in PCK cells. A higher shut-off in the host protein synthesis occurred in the virus-infected PCK cells than in the infected CE cells.
Department of Measles Virus, National Institute of Health, Tokyo, Japan.
Y
Y
Umino
T
T
Kohama
A
A
Sugiura
D000818:Animals; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002642:Chick Embryo; D009522:Newcastle disease virus / Q000235:genetics / Q000502:physiology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification; D013997:Time Factors; D010948:Viral Plaque Assay / Q000379:methods; D014764:Viral Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D014771:Virion / Q000235:genetics / Q000502:physiology; D014779:Virus Replication
D011506:Proteins; D014764:Viral Proteins