Effect of different MCT/LCT ratios on protein synthesis in injured rats fed parenterally.
The effect of the lipidic composition of the diet on the proteic synthesis of the male Sprague-Dawley rats fed parenterally for four days has been studied. All animals received identical nitrogen and caloric intake, but the ratio of medium to long-chain triglycerides (MCT/LCT) varied: group 1, control (0/100); group 2 (30/70); group 3 (50/50) and group 4 (70/30). Hepatic and jejunal protein synthesis were determined with L-(1-14C)-leucine. In groups 1, 3 and 4 muscle protein synthesis was measured with L-(3,4-3H)-phenylalanine. In liver, there were no significant differences in the fractional synthesis rate among the groups. In jejunum, the control group showed a higher fractional synthesis rate with statistically significant differences among the groups. In muscle, group 3 presented the highest fractional synthesis rate with statistically significant differences (p less than 0.05).
Servicio de Bioquímica Clínica Unidad Metabólica, Hospital General Vall d'Hebron, Barcelona, Spain.
S
S
Schwartz
M
M
Farriol
J
J
Balcells
J E
JE
Murio
E
E
García
E
E
Gemar
J
J
López
L A
LA
Andreu
D000818:Animals; D002149:Energy Intake; D007583:Jejunum / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis; D009584:Nitrogen / Q000378:metabolism; D010288:Parenteral Nutrition; D014176:Protein Biosynthesis; D051381:Rats; D013312:Stress, Physiological / Q000378:metabolism*; D014280:Triglycerides / Q000737:chemistry / Q000378:metabolism*
D009124:Muscle Proteins; D014280:Triglycerides; D009584:Nitrogen