1-Methylxanthine derived from theophylline as an in vivo biochemical probe of allopurinol effect.
The urinary 1-methyluric acid (1MU) to 1-methylxanthine (1MX) ratio has been assessed as a biochemical index of oxipurinol effect in vivo in man. Dosing with theophylline was used to produce 1MX as an intermediate metabolite in six healthy volunteers. A sigmoid Emax model was fitted to the data and gave a mean plasma oxipurinol IC50 of 3.0 +/- 1.1 mg l-1, a mean exponent n of 3.4 +/- 2.1 and a mean IC90 of 8.5 +/- 5.9 mg l-1. There was marked interindividual variability in the steepness of the plasma oxipurinol concentration response relationship, and in the plasma oxipurinol IC90 values. The study has confirmed the feasibility of using single doses of allopurinol to construct individual plasma oxipurinol concentration-response curves.
Department of Clinical Pharmacology, Flinders Medical Centre, Flinders University of South Australia, Adelaide.
D J
DJ
Birkett
J O
JO
Miners
R O
RO
Day
D000328:Adult; D000493:Allopurinol / Q000494:pharmacology*; D015415:Biomarkers; D005260:Female; D006801:Humans; D008297:Male; D010117:Oxypurinol / Q000097:blood; D013806:Theophylline / Q000378:metabolism*; D014527:Uric Acid / Q000031:analogs & derivatives / Q000097:blood; D014970:Xanthines / Q000097:blood / Q000378:metabolism*
D015415:Biomarkers; D014970:Xanthines; D014527:Uric Acid; C030530:1-methyluric acid; D000493:Allopurinol; C053085:1-methylxanthine; D013806:Theophylline; D010117:Oxypurinol