Creatine kinase BB produced by murine hybridomas but not by parental cells.
Creatine kinase BB is the main CK isoenzyme expressed in murine hybridoma cells as assessed by agarose gel electrophoresis whereas it was found neither in splenocytes nor in myeloma cells. The presence of CK BB is a constant finding in all 10 murine hybridomas examined to date irrespective of the specificity of the secreted antibodies.
Laboratoire d'Immunochimie, INSERM CJF 89-05, Faculté de Médecine Lyon-Sud, Oullins, France.
S
S
Legastelois
J P
JP
Steghens
J
J
Grange
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D003402:Creatine Kinase / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D006825:Hybridomas / Q000201:enzymology*; D007527:Isoenzymes; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma; D013154:Spleen / Q000201:enzymology
D007527:Isoenzymes; D003402:Creatine Kinase