cDNA cloning of a human androgen-induced mRNA exhibiting an early and protein synthesis-independent induction.
The detailed mechanism of action of androgens remains unknown. We have used an androgen-dependent human prostate cancer cell line and a subtractive cDNA hybridisation strategy to enrich for androgen-dependent sequences. This yielded a cDNA clone which exhibits the characteristics of a primary trans-activated target for androgens. This androgen-regulated gene encodes a polyadenylated 4.5 kb mRNA which is induced 30-50-fold within 3 h of treatment with 10 nM dihydrotestosterone. The induction does not require continued protein synthesis as it is maintained in the presence of protein synthesis inhibitors.
Colletta
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AA
Department of Clinical Biochemistry, University of Cambridge, Addenbrookes Hospital, UK.
Kealey
T
T
D000728:Androgens / Q000502:physiology*; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000302:isolation & purification; D003609:Dactinomycin / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D007700:Kinetics; D008297:Male; D011471:Prostatic Neoplasms; D011691:Puromycin / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D014407:Tumor Cells, Cultured
D000728:Androgens; D012333:RNA, Messenger; D003609:Dactinomycin; D011691:Puromycin; D004247:DNA; D003513:Cycloheximide