Identification of human esterase D subunits from the homodimeric and heterodimeric forms of five phenotypes by a new two-dimensional isoelectric focusing method.
A new two-dimensional isoelectric focusing method was developed to identify the human esterase D (EsD) subunits from the homodimeric and heterodimeric forms of five EsD phenotypes. EsD polymorphism was also analyzed by one-dimensional isoelectric focusing under reducing and mild denaturing conditions to study the influence of dithiothreitol and low concentrations of urea on the focusing pattern of the EsD dimers.
Alonso
A
A
Seccion de Biologia, Instituto Nacional de Toxicologia, Madrid, Spain.
Visedo
G
G
Sancho
M
M
Fernández-Piqueras
J
J
D000483:Alleles; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000097:blood* / Q000235:genetics; D002882:Chromosomes, Human, Pair 13; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology; D005796:Genes; D005819:Genetic Markers; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D007527:Isoenzymes / Q000097:blood* / Q000235:genetics; D010084:Oxidation-Reduction; D010641:Phenotype; D011489:Protein Denaturation
D005819:Genetic Markers; D007527:Isoenzymes; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C020118:ESD protein, human