Platelet-activating factor stimulates motion parameters of cryopreserved human sperm.
Platelet-activating factor is a naturally occurring molecule that does not appear to cause the detrimental effects to sperm as have been reported for other synthetic stimulatory agents. Incubation of freeze-thawed sperm with low motility with PAF at concentrations greater than or equal to 1.0 microM significantly improved a number of motion parameters (% motility, VSL and LIN). Optimal incubation conditions for PAF were defined at 1 hour and at 25 degrees C. The success of assisted reproductive techniques employing cryopreserved sperm with poor motility may be enhanced by stimulation protocols employing PAF.
Hellstrom
W J
WJ
Department of Urology, Tulane University School of Medicine, New Orleans, Louisiana.
Wang
R
R
Sikka
S C
SC
D015925:Cryopreservation; D006801:Humans; D008297:Male; D010972:Platelet Activating Factor / Q000494:pharmacology*; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*
D010972:Platelet Activating Factor