Virucidal levels of ozone induce hemolysis and hemoglobin degradation.
The animal virus, vesicular stomatitis virus (VSV), and the bacterial virus, phi 6, were inactivated by greater than 4 log10 in response to incubation with 13 to 14 mL of 1.4 mmol per L (65 micrograms/mL) to 1.6 mmol per L (75 micrograms/mL) of overlaid ozone in virus-spiked, dilute, red cell suspensions. Virus inactivation was greatly inhibited when ozone was overlaid in the presence of high-hematocrit red cells or, to a lesser degree, high levels of plasma. At hematocrits at which 5 to 6 log10 of VSV were inactivated, ozone caused 30-percent hemolysis, as measured by the loss of total cellular hemoglobin. Unexpectedly, this level of hemolysis could not be observed in supernatants because of the ozone-induced destruction (bleaching) of extracellular hemoglobin. These results suggest that ozone may have little biological specificity for damaging viruses over red cells.
Wagner
S J
SJ
Jerome H. Holland Laboratory for the Biomedical Sciences, American Red Cross, Rockville, Maryland.
Wagner
K F
KF
Friedman
L I
LI
Benade
L F
LF
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D015658:HIV Infections / Q000635:transmission; D006679:HIV Seropositivity / Q000378:metabolism; D015497:HIV-1 / Q000302:isolation & purification; D006454:Hemoglobins / Q000378:metabolism*; D006461:Hemolysis / Q000187:drug effects*; D016602:Needlestick Injuries / Q000150:complications; D010126:Ozone / Q000494:pharmacology*; D014709:Vero Cells / Q000382:microbiology*
D000998:Antiviral Agents; D006454:Hemoglobins; D010126:Ozone