The Staphylococcus aureus mutation pcrA3 leads to the accumulation of pT181 replication initiation complexes.
In Staphylococcus aureus cells carrying the pcrA3 chromosomal mutation, plasmid pT181 and its derivatives were maintained at a reduced copy number. A significant proportion of their DNA migrated during agarose gel electrophoresis as nicked DNA. The results obtained in the characterization of this plasmid DNA species show that it represents replication initiation complexes. Such complexes could not be detected in a wild-type host. The replication initiation complexes present in pcrA3 cells could resume replication after a lag. It was concluded from these results that the pcrA3 host mutation affected a step in plasmid pT181 replication immediately following the formation of the replication initiation complex, and that in pcrA3 this step became rate-limiting for plasmid pT181 replication.
Public Health Research Institute, New York, NY 10016.
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Iordanescu
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Basheer
D001426:Bacterial Proteins / Q000378:metabolism; D015153:Blotting, Western; D004261:DNA Replication / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D007700:Kinetics; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics*
D001426:Bacterial Proteins; C043650:RepC protein, Staphylococcus aureus