Isolation and characterization of a lectin from the mushroom, Lactarius deliciosus.
A lectin (LDL) has been isolated from carpophores of the edible mushroom, Lactarius deliciosus, using a combination of affinity chromatography on stromas of group O erythrocytes embedded in polyacrylamide gel and hydroxylapatite, and gel filtration chromatography. Its molecular weight, as determined by gel filtration, is about 37,000 and its structure is dimeric, with two distinct types of subunits (about 19,000 and 18,000). It appeared homogeneous on HPLC gel filtration, but exhibited microheterogeneity on isoelectric focusing. Amino acid analysis revealed that it contains a large amount of glycine. Hapten inhibition assaying indicated that the Lactarius lectin is most specific for D-Gal beta 1----3D-GalNAc. The lectin was found in the mycelium and its possible role in the fungus is discussed.
Department of Botany and Microbiology, Faculty of Pharmaceutical Sciences, University of Clermont I, Clermont-Ferrand, France.
J
J
Guillot
M
M
Giollant
M
M
Damez
M
M
Dusser
D000371:Agglutination / Q000187:drug effects; D000596:Amino Acids / Q000032:analysis; D001487:Basidiomycota / Q000737:chemistry*; D002241:Carbohydrates / Q000032:analysis; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects; D005656:Fungal Proteins / Q000032:analysis; D006386:Hemagglutination Tests; D006801:Humans; D006882:Hydroxyapatites; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight
D000596:Amino Acids; D002241:Carbohydrates; D005656:Fungal Proteins; D006882:Hydroxyapatites; D037102:Lectins