The effects of enflurane on the DNase I activity in an isolated enzyme preparation and in the DNase I-G actin complex.
The effects of enflurane on the DNase I activity in an isolated enzyme preparation and in the DNase I-G actin complex were investigated. DNase I, DNase I-G actin complexes and G actin were exposed to various (0.2-5.0 vol.%) enflurane concentrations for 180 min. Thereafter, DNase I activity was determined. Compared with controls, DNase I activity and the inhibitory capacity of G actin for DNase I were not affected by enflurane. However, there was a shift in the absorption maximum of DNase I after exposure to enflurane. The results suggest that enflurane alters the DNase I conformation without inhibition of DNase I function. In clinical subjects, side effects of anaesthesia can occur by interactions between hydrophobic anaesthetics and the hydrophobic groups of amino acids in proteins.
Reitz
M
M
Department of Physiological Chemistry, University of Mainz, FRG.
Leicher-Düber
A
A
Lanz
E
E
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002021:Buffers; D002417:Cattle; D003850:Deoxyribonuclease I / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004737:Enflurane / Q000494:pharmacology*; D010179:Pancreas / Q000201:enzymology
D000199:Actins; D002021:Buffers; D004737:Enflurane; D003850:Deoxyribonuclease I