[The effect of free radicals on the rabbit articular chondrocytes in monolayer culture].
By using monolayer culture technique, the effect of endogenous free radicals on rabbit articular chondrocytes was studied. The free radicals were provided through the action of xanthine oxidase (XO) on xanthine (X). The amount of DNA in chondrocytes was measured with ethidium bromide method for direct fluorometric estimation of DNA. The synthesis of proteoglycan (PG) was assayed through 35S-Na2SO4 incorporation followed by inverted microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The results indicated that chondrocytes could be damaged by endogenous free radicals generated by the action of XO on X, and these free radicals may be related to the pathogenesis of cartilage aging, degeneration, degenerative joint disease and arthritis.
Department of Pathology, Xian Medical University.
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Y
Kang
D000818:Animals; D002358:Cartilage, Articular / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D005609:Free Radicals; D011509:Proteoglycans / Q000096:biosynthesis; D011817:Rabbits; D014969:Xanthine Oxidase / Q000494:pharmacology; D014970:Xanthines / Q000494:pharmacology
D005609:Free Radicals; D011509:Proteoglycans; D014970:Xanthines; D004247:DNA; D014969:Xanthine Oxidase