[Polymerase chain reaction--principle and application in medicine].
The polymerase chain reaction, a new molecular technique, is of increasing importance in many areas of medical research and diagnosis. Within hours DNA sequences can be amplified millionfold with very high specificity, making detection and further analysis possible. Using multiple rounds of exponential amplification, even one copy of a gene of interest can be detected by agarose gel electrophoresis and other methods. Furthermore, minute amounts of DNA even from tissue damaged by embedding in paraffin can be analysed. Polymerase chain reaction methodology has already gained significance in many areas of medical research, e.g. diagnosis of inherited diseases, detection of viral DNA in clinical samples, cancer research and diagnosis, and characterization of gene expression.
Scharffetter
K
K
Dermatologische Klinik, Heinrich-Heine-Universität Düsseldorf.
Volkenandt
M
M
Sorg
R
R
Wlaschek
M
M
Eckes
B
B
D004279:DNA, Viral / Q000235:genetics; D005784:Gene Amplification; D015870:Gene Expression / Q000235:genetics; D030342:Genetic Diseases, Inborn / Q000175:diagnosis; D006680:HLA Antigens / Q000235:genetics; D006801:Humans; D009369:Neoplasms / Q000235:genetics; D016133:Polymerase Chain Reaction
D004279:DNA, Viral; D006680:HLA Antigens
Die Polymerasekettenreaktion--Prinzip und Anwendung in der Medizin.