Effect of dietary manipulation on c-myc RNA expression in adipose tissue, muscle and liver of broiler chickens.
The effects of dietary restriction on the relative steady state levels of cellular myc (c-myc) mRNA in abdominal adipose tissue, breast muscle and liver of chickens were determined. Fasting was found to increase c-myc RNA expression in adipose tissue (p less than 0.01). This increase returned to normal levels after refeeding. Muscle and liver in fasted birds did not show changes in c-myc that differed from controls. Serum concentrations of glucose, triglyceride (TG), free fatty acids (FFA) and insulin-like growth factor (IGF-I) were compared to levels of c-myc found in control birds. In adipose tissue, c-myc levels were negatively correlated with serum glucose, TG and IGF-I, while in muscle a positive correlation with serum glucose and TG was found. Data suggest that c-myc is involved in the metabolic changes occurring in fat cells under fasting conditions.
Kim
J W
JW
Department of Poultry Science, University of Georgia, Athens 30602.
Fletcher
D L
DL
Campion
D R
DR
Gaskins
H R
HR
Dean
R
R
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose; D002645:Chickens / Q000378:metabolism*; D004032:Diet; D005215:Fasting; D005230:Fatty Acids, Nonesterified / Q000097:blood; D015870:Gene Expression; D016259:Genes, myc; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D008099:Liver / Q000378:metabolism*; D008297:Male; D009132:Muscles / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D011863:Radioimmunoassay; D011897:Random Allocation; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D012333:RNA, Messenger; D014280:Triglycerides; D007334:Insulin-Like Growth Factor I