The in vivo release of 90Y from cyclic and acyclic ligand-antibody conjugates.
Yttrium binding ligands DOTA, caDTPA and CT-DTPA were each conjugated to monoclonal antibody B72.3, labelled with 90Y and injected into mice in order to assess the in vivo inertness of the antibody-linked 90Y-ligand complexes. Levels of 90Y in femur shafts of the DOTA-B72.3 mice were low, being approximately 7 and 44%, respectively, of levels in the femur shafts of the caDTPA-B72.3 and CT-DTPA-B72.3 treated mice. This finding demonstrates the greater inertness and by implication the greater suitability for immunotherapy of the DOTA-90Y complex.
MRC Radiobiology Unit, Chilton, Didcot, Oxon, England.
A
A
Harrison
C A
CA
Walker
D
D
Parker
K J
KJ
Jankowski
J P
JP
Cox
A S
AS
Craig
J M
JM
Sansom
N R
NR
Beeley
R A
RA
Boyce
L
L
Chaplin
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001853:Bone Marrow / Q000378:metabolism; D001842:Bone and Bones / Q000378:metabolism; D006571:Heterocyclic Compounds / Q000493:pharmacokinetics; D006573:Heterocyclic Compounds, 1-Ring; D007074:Immunoglobulin G / Q000276:immunology; D007553:Isotope Labeling; D008024:Ligands; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D004369:Pentetic Acid / Q000737:chemistry; D015021:Yttrium Radioisotopes / Q000493:pharmacokinetics*
D000911:Antibodies, Monoclonal; D006571:Heterocyclic Compounds; D006573:Heterocyclic Compounds, 1-Ring; D007074:Immunoglobulin G; D008024:Ligands; D015021:Yttrium Radioisotopes; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid; D004369:Pentetic Acid