Renal and hepatic microsomal enzymes responsible for bioactivation of 3-methoxy-4-aminoazobenzene in the rodent.
Activities of the renal and hepatic microsomal enzymes responsible for the N-hydroxylation and mutagenic activation of 3-methoxy-4-aminoazobenzene (3-MeO-AAB) were examined in male mice, rats, hamsters and guinea pigs. In all these rodent species, hepatic microsomes showed definite N-hydroxylation of 3-MeO-AAB, whereas the renal activity was detected only in mice. The hepatic enzyme responsible for N-hydroxylation of 3-MeO-AAB (3-MeO-AAB N-hydroxylase) was induced in all species except mice by phenobarbital and selectively in mice and hamsters by 3-methylcholanthrene, whereas these cytochrome P450 inducers did not affect the renal enzyme in mice, rats or hamsters. In individual microsome samples, activities for N-hydroxylation and mutagenic activation of 3-MeO-AAB correlated well. These results indicate that the renal and hepatic enzymes responsible for the metabolic activation of 3-MeO-AAB differed among different species of rodent animals in terms of their activity and inducibility with cytochrome P450 inducers.
Department of Hygienic Chemistry, Tohoku University, Sendai, Japan.
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S
Miura
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Degawa
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Y
Hashimoto
D000818:Animals; D001711:Biotransformation; D006224:Cricetinae; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004790:Enzyme Induction; D006168:Guinea Pigs; D006900:Hydroxylation; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008647:Mesocricetus; D008748:Methylcholanthrene / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D009152:Mutagenicity Tests; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D010128:p-Aminoazobenzene / Q000031:analogs & derivatives* / Q000378:metabolism
D008748:Methylcholanthrene; D010128:p-Aminoazobenzene; C044436:3-methoxy-4-aminoazobenzene; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital