Formation and crystallization of Thermus thermophilus 70S ribosome/tRNA complexes.
70S ribosomes from Thermus thermophilus are able to form ternary complexes with N-AcPhe-tRNAPhe from either Thermus thermophilus or Escherichia coli, in the presence of a short oligo(U) of six or nine uridines. A complex of N-AcPhe-tRNAPhe/(U)9/70S ribosome from Th. thermophilus was crystallized under the same conditions used for the growth of crystals from isolated ribosomes (S.D. Trakhanov, et al., (1987) FEBS Lett. 220, 319-322).
Institute of Protein Research, Academy of Sciences of the USSR, Pushchino.
G
G
Yusupova
M
M
Yusupov
A
A
Spirin
J P
JP
Ebel
D
D
Moras
C
C
Ehresmann
B
B
Ehresmann
D003461:Crystallography; D046911:Macromolecular Substances; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000648:ultrastructure; D012360:RNA, Transfer, Phe / Q000648:ultrastructure*; D012270:Ribosomes / Q000648:ultrastructure*; D016963:Thermus thermophilus / Q000648:ultrastructure*
D046911:Macromolecular Substances; D012333:RNA, Messenger; D012360:RNA, Transfer, Phe