Genetic studies of human apolipoproteins. XIX. Apolipoproteins E and A-IV phenotyping from whole blood and blood stains.
Apolipoproteins E and A-IV reveal common, genetically determined, structural polymorphisms, which can be detected in serum or plasma, using isoelectric focusing and immunoblotting techniques. In the present study, we have investigated the possibility of detecting the gene products of these two polymorphic loci in whole blood and blood stains. A total of 68 plasma samples and their corresponding whole blood samples were screened. Phenotypic patterns obtained in whole blood were identical to those obtained in plasma for both the APO E and APO A-IV polymorphisms. The signal-to-noise ratio in the APO E patterns was higher in whole blood than in fresh plasma. The gene products of both the APO E and APO A-IV were found to be very stable in several months old blood stains. The detection of apolipoprotein polymorphisms in whole blood and blood stains signifies their potential applications, on a wider scale, to several areas including forensic serology, paternity testing, clinical and population genetic studies.
Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pennsylvania 15261.
L J
LJ
Kelly
M I
MI
Kamboh
R E
RE
Ferrell
D001054:Apolipoproteins A / Q000235:genetics* / Q000302:isolation & purification; D001057:Apolipoproteins E / Q000097:blood / Q000235:genetics*; D001801:Blood Stains; D006801:Humans; D015151:Immunoblotting; D007525:Isoelectric Focusing; D010641:Phenotype; D011110:Polymorphism, Genetic
D001054:Apolipoproteins A; D001057:Apolipoproteins E; C022205:apolipoprotein A-IV