Phosphorylation and functional modification of calmodulin-dependent protein kinase IV by cAMP-dependent protein kinase.
Calmodulin-dependent protein kinase IV (CaM-kinase IV), a neuronal calmodulin-dependent multifunctional protein kinase, undergoes autophosphorylation in response to Ca2+ and calmodulin, resulting in activation of the enzyme (Frangakis et al. (1991) J. Biol. Chem. 266, 11309-11316). In contrast, the enzyme was phosphorylated by cAMP-dependent protein kinase, leading to a decrease in the enzyme activity. Thus, the results suggest differential regulation of CaM-kinase IV by two representative second messengers, Ca2+ and cAMP.
Kameshita
I
I
Department of Biochemistry, Asahikawa Medical College, Japan.
Fujisawa
H
H
D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002540:Cerebral Cortex / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins; D007700:Kinetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D013379:Substrate Specificity
D004791:Enzyme Inhibitors; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C063391:syntide-2; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C517686:Camk4 protein, rat