Polarographic determination of clavulanic acid.
A method is proposed for the determination of clavulanic acid by differential pulse polarography. The electroactive product was obtained by hydrolysis in sulphuric medium. It shows a reduction peak, that can be used analytically, at -0.75 V (vs SCE). The optimum conditions for the polarographic signal were determined and a study was made of the different parameters affecting the electrochemical process. A polarographic procedure is proposed for the determination of clavulanic acid in a concentration range of 8.0 X 10(-6) -1.4 X 10(-4) M. The detection limit is about 2 x 10(-6) M and the relative standard deviation is 1.1%. The method was applied to the determination of clavulanic acid in the presence of amoxicillin.
Departamento de Química Analitica, Facultad de Química, Universidad de Salamanca, Spain.
C
C
González Pérez
I
I
González Martín
B
B
Rodríguez Vázquez de Aldana
D000900:Anti-Bacterial Agents / Q000032:analysis*; D019818:Clavulanic Acid; D002969:Clavulanic Acids / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D011048:Polarography
D000900:Anti-Bacterial Agents; D002969:Clavulanic Acids; D019818:Clavulanic Acid