[Production of lectin from jack fruit (Artocarpus integrifolia) seeds and its interaction with carbohydrates and glycoproteins].
A method is developed to obtain lectin from jack fruit (Artocarpus integrifolia) seeds using an affinity chromatography on a sorbent prepared from the egg white. The minimum agglutination concentration of human erythrocytes is 80 ng/ml, the molecular weight of the preparation is about 39 kDa, it contains 1.8% of neutral hexoses and 3.1% of hexosamines. PAAG electrophoresis in the alkali system has revealed several molecular forms of lectin isolated by preparative electrophoresis, their properties are investigated. SDS-PAAG electrophoresis has revealed several types of polypeptide chains among which two chains (12 and 14 kDa) are predominant. Lectin possesses affinity to galactosides (not to free galactose) and N-acetylgalactosamine and interacts with O-glycans with high affinity. The preparation has mitogenic activity in optimal concentration 50 micrograms/ml.
Aberman
L E
LE
Petlychna
L I
LI
Lutsyk
M D
MD
D000116:Acetylgalactosamine / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005638:Fruit / Q000737:chemistry*; D005697:Galactosides / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D037102:Lectins / Q000302:isolation & purification* / Q000378:metabolism; D008934:Mitogens; D037121:Plant Lectins; D011134:Polysaccharides / Q000378:metabolism; D013379:Substrate Specificity
D005697:Galactosides; D006023:Glycoproteins; D037102:Lectins; D008934:Mitogens; D037121:Plant Lectins; D011134:Polysaccharides; D000116:Acetylgalactosamine
Oderzhannia lektynu iz nasinnia khlibnoho dereva (Artocarpus integrifoli) i ĭoho vzaiemodiia z vuglevodamy ta glikoproteïnamy.