The isolation and characterization of a calmodulin-encoding gene (CMD1) from the dimorphic fungus Candida albicans.
Candida albicans is a dimorphic, opportunistic pathogen of humans, and calcium and calmodulin have been implicated in its morphogenic transition. The C. albicans calmodulin-encoding gene, CMD1, was isolated from cDNA and genomic phage lambda libraries using the Saccharomyces cerevisiae CMD gene as a hybridization probe. Southern-blot hybridization analysis of genomic DNA suggests the existence of only one type of calmodulin gene in C. albicans. Comparison of cDNA and genomic sequences identified a 222-bp intron located immediately after the Met start codon. The predicted amino acid sequence was 60% identical with yeast CMD and 70% identical with CMDs of filamentous fungi and vertebrates. We have localized the CMD1 gene to chromosome 3 using the contour-clamped homogeneous electric field gel electrophoresis. The CMD1 gene hybridized to a single 650-nucleotide transcript which was present in equivalent amounts in both the yeast and hyphal forms of the organism.
Saporito
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Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine 92717.
Sypherd
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D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002147:Calmodulin / Q000235:genetics*; D002176:Candida albicans / Q000235:genetics*; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D016415:Sequence Alignment
D002147:Calmodulin; D004271:DNA, Fungal; D004247:DNA