[The effect of serum on NK cell activity].
In the immunosurveillance of the development and spread of tumors NK cells seem to have a major role. For this reason, the assessment of NK cell activity of patients with a malignant disease represents a significant immunological parameter. The results of our study indicate that for the group of 26 breast cancer patients basic NK cell activity is significantly below NK cell activity for healthy controls. In a previous set of experiments we found that a short term incubation of peripheral blood lymphocytes of these patients in medium, alone, gives a considerable augmentation of impaired basic NK activity, so we made an attempt to determine the influence of autologous serum on NK cell activity. Our data indicate that both healthy serum and autologous serum of breast cancer patients, in the early stage of disease, give a similar degree of inhibition of NK cell activity, after in vitro cultivation of PBL, when compared to PBL cultured in medium with FCS, and that only sera from breast cancer patients with advanced disease had a greater inhibiting effect. Incubation of PBL of healthy controls with autologous healthy sera showed a somewhat greater inhibitory effect on NK cell activity than sera from breast cancer patients in the early stage of disease. We consider that monitoring of NK cell activity after incubation with autologous serum may give not only a better assessment of NK cell activity of these patients in vivo, but also possibilities fro investigating factors which lead to serum-induced modulation of NK cell activity.
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G
Konjević
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I
Spuzić
D001790:Blood Physiological Phenomena; D001943:Breast Neoplasms / Q000097:blood / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*
Uticaj seruma na aktivnost NK celija.