Toxin production by atypical strains of Vibrio cholerae E1 Tor under different cultural conditions.
It was observed that at 37 degrees C under in vitro conditions, aerobic culture filtrates of a few strains of Vibrio cholerae biotype El Tor isolated from diarrhoeal cases produced a minute amount of toxin which failed to elicit a positive ileal loop reaction like toxigenic strains. Thus, these strains showed an atypical behaviour in their toxin producing ability. At 25 degrees C and 30 degrees C under aerobic cultural conditions enhanced toxin production was noticed in toxigenic strains, but these temperatures did not affect the toxigenicity of the atypical strains. The atypical Vibrio cholerae El Tor strains exhibited enhanced toxin production only at 37 degrees C under anaerobic conditions and the amount of toxin produced was akin to those of the toxigenic strains. In comparison to aerobic conditions, growth was observed to be comparatively lower under anaerobiosis both in the toxigenic and atpyical V. cholerae strains. Moreover, in contrast to the toxigenic strains, the toxin did not remain membrane-bound in these atypical strains at 37 degrees C and aerobic cultural conditions.
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.
M K
MK
Chakrabarti
S P
SP
De
A K
AK
Sinha
S C
SC
Pal
D000332:Aerobiosis / Q000502:physiology; D000693:Anaerobiosis / Q000502:physiology; D000818:Animals; D001295:Atypical Bacterial Forms / Q000378:metabolism; D001681:Biological Assay; D002462:Cell Membrane / Q000737:chemistry; D003470:Culture Media; D003967:Diarrhea / Q000382:microbiology; D004768:Enterotoxins / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D006358:Hot Temperature; D006801:Humans; D007194:India; D008565:Membrane Proteins / Q000378:metabolism; D011817:Rabbits; D014734:Vibrio cholerae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D004768:Enterotoxins; D008565:Membrane Proteins