Platelet-activating factor stimulates motion parameters of cryopreserved human sperm.
Platelet-activating factor is a naturally occurring molecule that does not appear to cause the detrimental effects to sperm as have been reported for other synthetic stimulatory agents. Incubation of freeze-thawed sperm with low motility with PAF at concentrations greater than or equal to 1.0 microM significantly improved a number of motion parameters (% motility, VSL and LIN). Optimal incubation conditions for PAF were defined at 1 hour and at 25 degrees C. The success of assisted reproductive techniques employing cryopreserved sperm with poor motility may be enhanced by stimulation protocols employing PAF.
Department of Urology, Tulane University School of Medicine, New Orleans, Louisiana.
W J
WJ
Hellstrom
R
R
Wang
S C
SC
Sikka
D015925:Cryopreservation; D006801:Humans; D008297:Male; D010972:Platelet Activating Factor / Q000494:pharmacology*; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*
D010972:Platelet Activating Factor