Transformation of the yeast Saccharomyces kluyveri by Saccharomyces cerevisiae-based plasmids.
For the transformation of the yeast Saccharomyces kluyveri, ura3 mutants were obtained by 5-fluoro-orotic acid selection. By utilizing the method based on treatment of intact cells with alkali cations, the ura3 strains of S. kluyveri were transformed by Saccharomyces cerevisiae-based plasmids. In the transformed cells, a S. cerevisiae centromere-based plasmid was stably replicated autonomously. Thus, this system will permit the study of gene expression and its regulation in S. kluyveri in relationship to that in S. cerevisiae.
Laboratory for Molecular Biology, Pharma Research Laboratories, Hoechst Japan Limited, Kawagoe.
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Fujimura
D000667:Ampicillin / Q000494:pharmacology; D000668:Ampicillin Resistance / Q000235:genetics; D002503:Centromere / Q000502:physiology; D004587:Electrophoresis, Agar Gel; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D009154:Mutation / Q000235:genetics; D009963:Orotic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics*; D012440:Saccharomyces / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014170:Transformation, Genetic / Q000235:genetics*; D014498:Uracil / Q000494:pharmacology
D014498:Uracil; D009963:Orotic Acid; D000667:Ampicillin; C001242:5-fluoroorotic acid; D005020:Ethyl Methanesulfonate