Localization of the human tissue factor recognition determinant of human factor VIIa.
Tissue factor is an integral membrane glycoprotein that serves as an essential cofactor for the blood coagulation factor VIIa. Recent studies have attempted to localize the tissue factor recognition determinant of human factor VIIa. While several regions of factor VIIa have been implicated as important for tissue factor binding, the high affinity tissue factor recognition determinant of human factor VIIa is unknown. In order to define the determinant, we constructed a set of six chimeric proteins composed of portions of factor VII and factor IX. We then utilized the chimeras in competition experiments with 125I-labeled factor VIIa for recombinant tissue factor bound to an Immobilon-P membrane. The data indicate that the high affinity tissue factor recognition determinant of human factor VIIa is within the epidermal growth factor domains.
Toomey
J R
JR
Department of Biology, University of North Carolina, Chapel Hill 27599.
Smith
K J
KJ
Stafford
D W
DW
D001483:Base Sequence; D002678:Chimera; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004815:Epidermal Growth Factor / Q000235:genetics; D015942:Factor VIIa / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016133:Polymerase Chain Reaction; D013925:Thromboplastin / Q000378:metabolism*
D004815:Epidermal Growth Factor; D004247:DNA; D013925:Thromboplastin; D015942:Factor VIIa