Generation of recombinant baculovirus via liposome-mediated transfection.
Baculovirus expression vectors have become a popular method of producing recombinant proteins. Production of recombinant virus requires the transfection of both the native viral DNA and a transfer plasmid into insect cells where recombination takes place. While several methods of transfecting insect cells exist, we have found liposome-mediated transfection to be highly efficient. Here we detail the protocols and medium needed for efficient, simple transfection of Spodoptera frugiperda cells.
Hartig
P C
PC
NSI Environmental Sciences, Research Triangle Park, NC 27709.
Cardon
M C
MC
Kawanishi
C Y
CY
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D008081:Liposomes; D009036:Moths; D011995:Recombination, Genetic; D014162:Transfection
D008081:Liposomes