Tetrahymena thermophila acidic ribosomal protein L37 contains an archaebacterial type of C-terminus.
We have cloned and characterized a Tetrahymena thermophila macronuclear gene (L37) encoding the acidic ribosomal protein (A-protein) L37. The gene contains a single intron located in the 3'-part of the coding region. Two major and three minor transcription start points (tsp) were mapped 39 to 63 nucleotides upstream from the translational start codon. The uppermost tsp mapped to the first T in a putative T. thermophila RNA polymerase II initiator element, TATAA. The coding region of L37 predicts a protein of 109 amino acid (aa) residues. A substantial part of the deduced aa sequence was verified by protein sequencing. The T. thermophila L37 clearly belongs to the P1-type family of eukaryotic A-proteins, but the C-terminal region has the hallmarks of archaebacterial A-proteins.
Department of Molecular Biology, University of Odense, Denmark.
T S
TS
Hansen
P H
PH
Andreasen
H
H
Dreisig
P
P
Højrup
H
H
Nielsen
J
J
Engberg
K
K
Kristiansen
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D016054:DNA, Protozoan; D008969:Molecular Sequence Data; D005810:Multigene Family; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics*; D016808:Tetrahymena thermophila / Q000235:genetics*; D014158:Transcription, Genetic
D016054:DNA, Protozoan; D012269:Ribosomal Proteins