Phenotyping of bovine milk proteins by reversed-phase high-performance liquid chromatography.
A reversed-phase high-performance liquid chromatographic method for the separation of the most common and some less common genetic variants of the bovine caseins is described. When the method is used for analysing clarified skim milk, simultaneous identification of casein variants and major they protein variants can be effected in a single run. The potential of the method for quantitative application is discussed.
Department of Biophysical Chemistry, Netherlands Institute for Dairy Research NIZO, Ede.
S
S
Visser
C J
CJ
Slangen
H S
HS
Rollema
D000818:Animals; D002364:Caseins / Q000032:analysis; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D005612:Freeze Drying; D005838:Genotype; D007525:Isoelectric Focusing; D008894:Milk Proteins / Q000032:analysis* / Q000235:genetics; D010641:Phenotype; D000067816:Whey Proteins
D002364:Caseins; D008894:Milk Proteins; D000067816:Whey Proteins