Gastrin releasing peptide-like immunoreactive substance in rat mammary glands during pregnancy.
The concentration of gastrin releasing peptide-like immunoreactive substance (GRP-IS) was measured by enzyme immunoassay (EIA) in rat mammary glands during pregnancy and after delivery. The GRP-IS concentration was high in the middle stage of pregnancy (10-14 days of gestation) and decreased in late pregnancy to reach a plateau range. By using HPLC, it was shown that GRP(20-29) and GRP(16-29) were mainly present in rat mammary glands. Immunohistochemical study revealed that epithelial cells of rat mammary glands were stained intensely with antiserum GP-6201. These results suggest that GRP-IS is produced and secreted in epithelial cells of mammary glands and takes part in the proliferation, differentiation and hypertrophy of mammary glands.
Department of Clinical Pharmacy, Medical College of Oita, Japan.
M
M
Takeyama
K
K
Kondo
A
A
Otaka
S
S
Asakura
N
N
Morikawa
S
S
Itami
S
S
Takayasu
N
N
Fujii
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004848:Epithelium / Q000378:metabolism; D005260:Female; D019886:Gastrin-Releasing Peptide; D005755:Gastrins / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008321:Mammary Glands, Animal / Q000378:metabolism*; D010455:Peptides / Q000032:analysis / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains
D005755:Gastrins; D010455:Peptides; D019886:Gastrin-Releasing Peptide