Antibody quantitation in seconds using affinity Perfusion Chromatography.
An extremely rapid assay technique for antibodies has been developed utilizing protein A or protein G bound to Perfusion Chromatography support matrices. Either dilute or concentrated samples are directly injected on a column that selectively binds antibody, which is quantitated directly by elution and UV absorbance. Due to the unique mass transport characteristics of the supports, total assay cycle times are typically 1 minute or less, with assays as short as 15 seconds possible. The assay system can accurately quantitate a 100,000:1 or greater dynamic range in sample concentration without sample dilution, is extremely repeatable and is easy to automate with conventional HPLC systems. Assay of antibodies in a wide range of sample types has been demonstrated.
Fulton
S P
SP
PerSeptive Biosystems, Inc., University Park at MIT, Cambridge, MA 02139.
Meys
M
M
Várady
L
L
Jansen
R
R
Afeyan
N B
NB
D000818:Animals; D002138:Calibration; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D008024:Ligands; D051379:Mice; D009419:Nerve Tissue Proteins; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013205:Staphylococcal Protein A
C029152:G-substrate; D007074:Immunoglobulin G; D008024:Ligands; D009419:Nerve Tissue Proteins; D013205:Staphylococcal Protein A