A new class of anti-HIV agents: GAP31, DAPs 30 and 32.
Three inhibitors of human immunodeficiency virus (HIV) have been isolated and purified to homogeneity from Euphorbiaceae himalaya seeds (Gelonium multiflorum) and carnation leaves (Dianthus caryophyllus). These proteins, GAP 31 (Gelonium Anti-HIV Protein 31 kDa) and DAPs 30 and 32 (dianthus anti-HIV proteins, 30 and 32 kDa), inhibit HIV-1 infection and replication in a dose-dependent manner with little toxicity to target cells. The therapeutic indices of these compounds are in the order 10(4), suggesting that they may be clinically important agents in the treatment of AIDS. The N-terminal amino acid sequences of these proteins show little homology to those of previously described anti-HIV proteins. The structure-function features of these HIV inhibitors, based on the 40-60 amino acid residues of N-terminal sequences, are examined.
Department of Biochemistry, New York University School of Medicine, NY 10016.
S
S
Lee-Huang
H F
HF
Kung
P L
PL
Huang
P L
PL
Huang
B Q
BQ
Li
P
P
Huang
H I
HI
Huang
H C
HC
Chen
D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000633:toxicity; D004591:Electrophoresis, Polyacrylamide Gel; D006678:HIV / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000187:drug effects; D016415:Sequence Alignment; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; C071381:DAP30 protein, D caryophyllus; C071382:DAP32 protein, D caryophyllus; C071380:GAP31 protein, Gelonium multiflorum; D010936:Plant Extracts; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1