Calcitonin gene-related peptide increases the production of glycosaminoglycans but not of collagen type I and III in cultures of rat fat-storing cells.
We investigated whether calcitonin gene-related peptide (CGRP) was able to affect the production of collagen and glycosaminoglycans (GAG) in cultures of rat fat-storing cells (FSC). Rat CGRP (1 nM-1 microM) induced a dose-dependent increase of total GAG production in FSC cultures with an EC50 of 28 nM. One uM human CGRP (8-37) shifted the dose-response curve of rat CGRP to the right (EC50 = 257 nM) without depressing the maximal response. Salmon calcitonin (1 nM-1 microM) did not significantly modify total GAG accumulation in FSC cultures. Collagen type I and III production was not significantly affected by either CGRP or calcitonin in FSC cultures. These findings suggest that peripheral sensory neuropeptides may modulate liver fibrogenesis.
Casini
A
A
Dip. Fisiopatologia Clinica, Università di Firenze, Italy.
Galli
G
G
Salzano
R
R
Santicioli
P
P
Maggi
C A
CA
Rotella
C M
CM
Surrenti
C
C
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002116:Calcitonin / Q000494:pharmacology; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D005260:Female; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D006025:Glycosaminoglycans; C028815:salmon calcitonin; D002116:Calcitonin; D003094:Collagen; D015740:Calcitonin Gene-Related Peptide