Dry rehydratable film for enumeration of total coliforms and Escherichia coli in foods: collaborative study.
Rehydratable dry-film plating methods for total coliforms and Escherichia coli in foods have been compared to the AOAC most probable number methods. Fourteen laboratories participated in the collaborative study. Three coliform and E. coli levels in 6 samples of 4 product types (flour, nuts, cheese, and beef with gravy) and in 3 samples of 2 product types (mushrooms and raw turkey) were tested in duplicate by the participants. The mean log counts for the 3 methods were comparable. In general, the repeatability and reproducibility variances of the plating methods were as good as or better than that of the MPN method. The method has been adopted official first action by AOAC.
Silliker Laboratories, Inc., Chicago Heights, IL 60411.
M S
MS
Curiale
T
T
Sons
D
D
McIver
J S
JS
McAllister
B
B
Halsey
D
D
Roblee
T L
TL
Fox
D000818:Animals; D001487:Basidiomycota / Q000737:chemistry; D002417:Cattle; D002611:Cheese / Q000032:analysis; D015169:Colony Count, Microbial / Q000295:instrumentation; D004755:Enterobacteriaceae / Q000254:growth & development*; D004926:Escherichia coli / Q000254:growth & development*; D005433:Flour / Q000032:analysis; D005516:Food Microbiology; D008460:Meat / Q000032:analysis; D009754:Nuts / Q000737:chemistry / Q000382:microbiology; D014422:Turkeys