Evaluation of cell attachment matrix (CAM) coated plates for primary culture of human tumour biopsies.
Baker et al. [Cancer Res. 46: 1263-1274, 1986] developed an adhesive tumour cell culture system (ATCCS) using a culture surface coated with a cell attachment matrix (CAM). The ability of CAM-coated plates to support the growth of cells from human tumour biopsies has been evaluated. Successful growth was obtained in 9/22 samples (41%), but fibroblasts, rather than tumour cells, grew in the majority. Comparison of CAM with other surfaces showed that CAM was no better for establishing tumour cell growth than the presence of feeder cells or an alternative attachment factor vitronectin.
Radiotherapy Research Unit, Institute of Cancer Research, Sutton, Surrey, U.K.
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P
Price
C
C
Bush
C S
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Parkins
T J
TJ
McMillan
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M
Robinson
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GG
Steel
D000818:Animals; D001706:Biopsy; D002448:Cell Adhesion / Q000528:radiation effects; D002455:Cell Division / Q000528:radiation effects; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D066298:In Vitro Techniques; D009369:Neoplasms / Q000473:pathology*; D009374:Neoplasms, Experimental / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000528:radiation effects*