Natural abundance 13C-nuclear magnetic resonance spectroscopic analysis of acyclic polyol and trehalose accumulation by several yeast species in response to salt stress.
Analysis of seventeen yeast strains by 13C-NMR spectroscopy has confirmed the significance of glycerol as the sole osmoregulatory solute under salt-stressed conditions, and has shown arabitol to be present in most of the osmotolerant species. Ribitol was detected in some species, including Debaryomyces hansenii, although ribitol accumulation did not correlate with the osmotic pressure of the medium. Relative amounts of arabitol and ribitol decreased in relation to glycerol when the external osmotic pressure was increased. Trehalose was present during exponential growth of some species.
Department of Biological Sciences, University of Dundee, Scotland, U.K.
A J
AJ
Meikle
J A
JA
Chudek
R H
RH
Reed
G M
GM
Gadd
D005990:Glycerol / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D012255:Ribitol / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D013402:Sugar Alcohols / Q000378:metabolism*; D014199:Trehalose / Q000378:metabolism*; D014882:Water-Electrolyte Balance / Q000187:drug effects / Q000502:physiology; D015003:Yeasts / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
D013402:Sugar Alcohols; D012965:Sodium Chloride; D012255:Ribitol; D014199:Trehalose; D005990:Glycerol; C014999:arabitol