Segregation of genes from donor strain during the production of recombinant congenic strains.
Recombinant congenic strains (RCS) constitute a set of inbred strains which are designed to dissect the genetic control of multigenic traits, such as tumour susceptibility or disease resistance. Each RCS contains a small fraction of the genome of a common donor strain, while the majority of genes stem from a common background strain. We tested at two stages of the inbreeding process in 20 RCS, derived from BALB/cHeA and STS/A, to see whether alleles from the STS/A donor strain are distributed over the RCS in a ratio as would theoretically be expected. Four marker genes (Pep-3; Pgm-1; Gpi-1 and Es-3) located at 4 different chromosomes were selected and the allelic distribution was tested after 3-4 and after 12 generations of inbreeding. The data obtained do not significantly deviate from the expected pattern, thus supporting the validity of the concept of RCS.
Department of Laboratory Animal Science, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
L F
LF
van Zutphen
M
M
Den Bieman
A
A
Lankhorst
P
P
Demant
D000483:Alleles; D000818:Animals; D002875:Chromosomes / Q000201:enzymology; D003433:Crosses, Genetic; D005796:Genes; D005819:Genetic Markers; D007178:Inbreeding; D051379:Mice; D008807:Mice, Inbred BALB C; D011995:Recombination, Genetic
D005819:Genetic Markers