Patients with combined hypercholesterolemia-hypertriglyceridemia show an increased monocyte-endothelial cell adhesion in vitro: triglyceride level as a major determinant.
Hypercholesterolemia (HC) is one of the primary risk factors for atherosclerosis. Patients with familial hypercholesterolemia (FH) or combined hypercholesterolemia-hypertriglycerinemia (CHH) are at risk to develop premature atherosclerosis. Animal models have revealed that diet-induced HC in vivo leads to an increased adhesion of monocytes to the endothelium of the vessel wall. Changes in the monocytes, endothelial cells, or serum components may lead to the increased monocyte adhesion that results in atherosclerotic plaque formation. In the present study, we investigated the binding of the monocyte in an in vitro system. Incubation of freshly isolated monocytes from CHH patients with cultured human umbilical vein endothelial cells (HUVEC) gave a significant 60% increase in monocyte adhesion when compared with monocytes from healthy subjects. No such increase was observed using monocytes from nontreated FH patients. These data suggest that CHH results in in vivo alterations of the monocytes that lead to an increased in vitro adhesion to HUVEC, and that an increased level of plasma triglycerides is the major determinant, since HC alone does not induce this alteration.
MGC-Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
M
M
de Gruijter
N
N
Hoogerbrugge
M A
MA
van Rijn
J F
JF
Koster
W
W
Sluiter
J F
JF
Jongkind
D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D006801:Humans; D006937:Hypercholesterolemia / Q000150:complications / Q000503:physiopathology*; D015228:Hypertriglyceridemia / Q000150:complications / Q000503:physiopathology*; D009000:Monocytes / Q000502:physiology*; D012016:Reference Values; D012044:Regression Analysis; D014280:Triglycerides / Q000097:blood*
D014280:Triglycerides