Recovery of the simian immunodeficiency virus (SIV) and depression of colony formation in in vitro infected progenitor cell-enriched rhesus bone marrow (BM).
Rhesus progenitor-enriched BM was exposed overnight to SIV and cultured in a limiting dilution assay where the potential for progenitor interaction with lymphocytes or macrophages was low. Virus was consistently isolated late in culture, detection being aided by coculture with CEM174 lymphoblasts. Although infected cells had reduced clonogenic activity, colonies were indistinguishable from those derived from uninfected BM with respect to proliferative potential, morphology, and longevity in culture. Primate immunodeficiency viruses, therefore, may infect immature BM populations, directly affecting hematopoietic activity.
Oncology Center, School of Medicine, Johns Hopkins University, Baltimore, MD.
L
L
Beltz
O
O
Narayan
R J
RJ
Adams
S J
SJ
Noga
A D
AD
Donnenberg
D000818:Animals; D001853:Bone Marrow / Q000382:microbiology*; D001854:Bone Marrow Cells; D016923:Cell Death; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000382:microbiology*; D008253:Macaca mulatta; D008264:Macrophages / Q000382:microbiology; D009000:Monocytes / Q000382:microbiology; D016012:Poisson Distribution; D011336:Probability; D015302:Simian Immunodeficiency Virus / Q000302:isolation & purification* / Q000502:physiology; D013601:T-Lymphocytes / Q000382:microbiology; D014779:Virus Replication