Theoretical considerations of the Tsou plot.
A graphical method was proposed by Tsou in 1962 for interpreting the data obtained by chemical modification of proteins and determining the number of essential groups involved. A strict mathematical proof and the estimation of deviation probability for this method are presented here. From the well-known Chebyshev's inequality, it has been shown that the possible error which could be derived from the Tsou plot will be much smaller than the usual experimental error obtainable. Some problems related to experiment and to application of Tsou plot in oligomeric enzymes have been discussed. Some analysis of experimental data taken from the literature are presented.
Institute of Biophysics, Academia Sinica, Beijing, China.
Z X
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Wang
D000818:Animals; D004798:Enzymes / Q000737:chemistry*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry; D008433:Mathematics; D009132:Muscles / Q000201:enzymology; D011817:Rabbits; D013329:Structure-Activity Relationship; D013438:Sulfhydryl Compounds / Q000737:chemistry
D004798:Enzymes; D013438:Sulfhydryl Compounds; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases