Regulation of differentially spliced transcripts of acyl-CoA oxidase in the rat.
RNAse protection assay was used to distinguish between and to quantify alternatively spliced transcripts of acyl-CoA oxidase in liver, kidney and testis of control and methylclofenapate treated rats. The ratio of spliced transcripts (type I to II) was 1.18:1 in control liver RNA, with 130 and 110 molecules/cell, respectively, and 3.1:1 in treated liver RNA, with 2800 and 900 molecules/cell. The ratios were 1.6 and 2:1 in control and treated kidney, and 0.31:1 in testis. This is likely to be due to differential splicing, which is, therefore, regulated during peroxisome proliferation, and also in a tissue specific fashion.
Department of Zoology, University of Nottingham, U.K.
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Bell
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Elcombe
D042962:Acyl-CoA Oxidase; D000818:Animals; D001483:Base Sequence; D002992:Clofenapate / Q000494:pharmacology; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012333:RNA, Messenger / Q000302:isolation & purification; D051381:Rats; D013737:Testis / Q000187:drug effects / Q000201:enzymology
D012333:RNA, Messenger; D002992:Clofenapate; D010088:Oxidoreductases; D042962:Acyl-CoA Oxidase