In vivo effect of the tus mutation on cell division in an Escherichia coli strain where chromosome replication is under the control of plasmid R1.
The phenotypic effect of the tus::kan mutation in an Escherichia coli strain, where the chromosome is replicated unidirectionally by an integrated R1 miniplasmid, was examined by flow cytometry and phase fluorescence microscopy. The tus+ cells exhibited perturbed cell division, as indicated by the presence of many elongated cells and filaments. Inactivation of the tus gene led to a reduction in the frequency of such elongated cells, presumably by eliminating Tus-mediated polar arrests of replication forks at ter sites, thereby shortening the time required for completion of chromosome replication.
Department of Microbiology, Uppsala University, Sweden.
S
S
Dasgupta
R
R
Bernander
K
K
Nordström
D001426:Bacterial Proteins / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D002876:Chromosomes, Bacterial; D004261:DNA Replication / Q000235:genetics; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D009154:Mutation; D010641:Phenotype; D011815:R Factors
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C061910:tus protein, Bacteria; C489236:tus protein, E coli