A rapid (less than 10 minute) electrophoresis method for identification of wheat varieties.
Conventional procedures for electrophoretic identification of grain samples according to variety are too slow to permit checking at the time of delivery. The method described permits electrophoretic identification within an hour. It involves extraction of gliadin proteins from crushed grain with 6% urea solution or ethylene glycol, cathodic electrophoresis for 9 min at 300 V in a Micrograd gel (MG 315 from Gradipore Ltd, Sydney, Australia) using sodium lactate buffer (pH 3.1), and staining in Gradipore (at about 50 degrees C). Distinction between a set of Australian varieties was similar to that obtainable with the Australian Standard Procedure.
CSIRO Wheat Research Unit, Division of Plant Industry, North Ryde, NSW, Australia.
C W
CW
Wrigley
P J
PJ
Gore
H P
HP
Manusu
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005433:Flour / Q000032:analysis; D005903:Gliadin / Q000032:analysis*; D008832:Microchemistry / Q000379:methods; D014908:Triticum / Q000032:analysis / Q000145:classification*
D005903:Gliadin