Segregation of genes from donor strain during the production of recombinant congenic strains.
Recombinant congenic strains (RCS) constitute a set of inbred strains which are designed to dissect the genetic control of multigenic traits, such as tumour susceptibility or disease resistance. Each RCS contains a small fraction of the genome of a common donor strain, while the majority of genes stem from a common background strain. We tested at two stages of the inbreeding process in 20 RCS, derived from BALB/cHeA and STS/A, to see whether alleles from the STS/A donor strain are distributed over the RCS in a ratio as would theoretically be expected. Four marker genes (Pep-3; Pgm-1; Gpi-1 and Es-3) located at 4 different chromosomes were selected and the allelic distribution was tested after 3-4 and after 12 generations of inbreeding. The data obtained do not significantly deviate from the expected pattern, thus supporting the validity of the concept of RCS.
van Zutphen
L F
LF
Department of Laboratory Animal Science, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Den Bieman
M
M
Lankhorst
A
A
Demant
P
P
D000483:Alleles; D000818:Animals; D002875:Chromosomes / Q000201:enzymology; D003433:Crosses, Genetic; D005796:Genes; D005819:Genetic Markers; D007178:Inbreeding; D051379:Mice; D008807:Mice, Inbred BALB C; D011995:Recombination, Genetic
D005819:Genetic Markers