Preparation and characterization of immunoliposomes for targeting of antiviral agents.
Antibodies specific to avian myeloblastosis virus envelope glycoprotein gp80 were raised. Immunoliposomes were prepared using anti-avian myeloblastosis virus envelope glycoprotein gp80 antibody. The antibody was palmitoylated to facilitate its incorporation into lipid bilayers of liposomes. The fluorescence emission spectra of palmitoylated IgG have exhibited a shift in emission maximum from 330 to 370 nm when it was incorporated into the liposomes. At least 50% of the incorporated antibody molecules were found to be oriented towards the outside in the liposomes. The average size of the liposome was found to be 300 A, and on an average, 15 antibody molecules were shown to be present in a liposome. When adriamycin encapsulated in immunoliposomes was incubated in a medium containing serum for 72 h, about 75% of the drug was retained in liposomes. In vivo localization studies, revealed an enhanced delivery of drug encapsulated in immunoliposomes to the target tissue, as compared to free drug or drug encapsulated in free liposomes. These data suggest a possible use of the drugs encapsulated in immunoliposomes to deliver the drugs in target areas, thereby reducing side effects caused by antiviral agents.
Dept. of Molecular Biology, Cleveland Clinic Foundation, OH 44195.
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Kalvakolanu
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A
Abraham
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000998:Antiviral Agents / Q000008:administration & dosage*; D001353:Avian Leukosis / Q000188:drug therapy; D009189:Avian Myeloblastosis Virus / Q000276:immunology*; D002499:Centrifugation, Density Gradient; D002645:Chickens; D004317:Doxorubicin / Q000008:administration & dosage / Q000627:therapeutic use; D004337:Drug Carriers; D007074:Immunoglobulin G / Q000276:immunology; D008081:Liposomes; D019308:Palmitic Acid; D010169:Palmitic Acids; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D014759:Viral Envelope Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000998:Antiviral Agents; D004337:Drug Carriers; D007074:Immunoglobulin G; D008081:Liposomes; D010169:Palmitic Acids; D014759:Viral Envelope Proteins; D019308:Palmitic Acid; D004317:Doxorubicin