Association of p21ras with cellular polypeptides.
p21ras specific antiserum was used to immunoprecipitate p21ras polypeptides from human A431 cells. In addition to p21ras, this antiserum precipitated a series of polypeptides with relative molecular weights of 150,000, 120,000, 105,000, and 50,000. The precipitation of these polypeptides was prevented by preincubation of the antiserum with an excess of purified Ras protein. These polypeptides do not share an epitope with p21ras, and two of them (120 and 150 kDa) copurify with a fraction of p21ras. The co-precipitation of p21ras with these polypeptides was detected in a variety of cell types. The pattern of the immunoprecipitates was consistently different in normal and ras-transformed cells. The 120- and 150-kDa polypeptides are phosphorylated on serine and threonine in A431 cells. Serum treatment resulted in a 2-fold increase in the phosphoserine content of the 120-kDa polypeptides.
Kaplan
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Cold Spring Harbor Laboratory, New York 11724-2204.
Bar-Sagi
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D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002461:Cell Line, Transformed; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D010455:Peptides / Q000378:metabolism*; D011233:Precipitin Tests; D016283:Proto-Oncogene Proteins p21(ras) / Q000276:immunology / Q000378:metabolism*; D051381:Rats
D000911:Antibodies, Monoclonal; D010455:Peptides; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)