Comparative inhibition of hepatitis B virus DNA polymerase and cellular DNA polymerases by triphosphates of sugar-modified 5-methyldeoxycytidines and of other nucleoside analogs.
Of a series of 14 nucleoside 5'-triphosphates, those of 2',3'-dideoxy-3'-fluoro-5-methylcytidine, 3'-azido-2',3'-dideoxy-5-methylcytidine, 2',3'-dideoxy-3'-fluoroguanosine, 2',3'-didehydro-2',3'-dideoxy-5-methylcytidine, 2',3'-dideoxy-3'-fluoro-5-ethylcytidine, and 2',3'-dideoxy-3'-fluoroadenosine emerged as the most potent inhibitors of hepatitis B virus DNA polymerase (50% inhibitory dose, 0.03 to 0.35 microM). In contrast, cellular DNA polymerases proved to be resistant to (alpha) or partially affected by (beta) these analogs. These compounds are among the most effective and selective inhibitors of endogenous hepatitis B virus DNA polymerase recognized to date.
Institut für Molekularbiologie, Humboldt-Universität Berlin (Charité), Germany.
E
E
Matthes
K
K
Reimer
M
M
von Janta-Lipinski
H
H
Meisel
C
C
Lehmann
D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006515:Hepatitis B virus / Q000201:enzymology*; D007700:Kinetics; D019384:Nucleic Acid Synthesis Inhibitors; D009705:Nucleosides / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology
D019384:Nucleic Acid Synthesis Inhibitors; D009705:Nucleosides; D010710:Phosphates; D003841:Deoxycytidine; C016569:5-methyldeoxycytidine