Differences in level and avidity of secretory IgA antibodies in breast milk of Swedish, Indian and Japanese mothers to soybean protein.
Colostrum was collected from Swedish, Indian and Japanese mothers. The samples were as a mean, collected 4.00-4.25 days after delivery of term infants. The level of specific IgA antibody to 2S, 7S and crude soybean antigen were measured by the enzyme-linked immunosorbent assay (ELISA). The avidity of the IgA antibodies to 7S soybean antigen was also measured with an ELISA system using different molarities of potassium thiocyanate for elution of the specific IgA antibody from solid phase-bound antigen. The level of specific IgA antibody to 7S and crude soybean antigen in the milk of the Indian mothers was significantly higher than in the milk of the Japanese mothers (p less than or equal to 0.01). In contrast, the avidity expressed as the molarity of KSCN for 50% elution of IgA antibody to 7S soybean antigen in the milk of the Japanese mothers was significantly higher than in the milk of the Indian mothers (p less than 0.01).
Department of Clinical Immunology, University of Göteborg, Sweden.
A
A
Morikawa
U
U
Dahlgren
B
B
Carlsson
I
I
Narayanan
M
M
Hahn-Zoric
L A
LA
Hanson
S
S
Maeda
S
S
Tomizawa
T
T
Kuroume
D000915:Antibody Affinity / Q000276:immunology*; D052179:Antigens, Plant; D003126:Colostrum / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005916:Globulins / Q000276:immunology; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D007194:India; D007564:Japan; D008895:Milk, Human / Q000737:chemistry*; D014674:Plant Proteins, Dietary / Q000276:immunology*; D011247:Pregnancy; D055314:Seed Storage Proteins; D030262:Soybean Proteins; D013548:Sweden
D052179:Antigens, Plant; D005916:Globulins; D007071:Immunoglobulin A, Secretory; D014674:Plant Proteins, Dietary; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max