An enzymeimmunoassay method for thyroxine determination in serum samples.
Here we describe an enzymeimmunoassay (EIA) for thyroxine (T4) in serum, whose performance is comparable to that of a sensitive T4 radioimmunoassay (RIA). In this assay, specific T4 antibody adsorbed on polystyrene beads is used along with T4-horseradishperoxidase as the tracer and omicron-phenylenediamine as chromogen. Several samples were analysed both by this T4 EIA and by using a commercial RIA kit for T4. The results correlate well with a correlation coefficient r = 0.9 and slope = 0.93 (n = 50).
Korde
A
A
Isotope Division, Bhabha Atomic Research Centre, Bombay, India.
Meera
V
V
D006801:Humans; D007124:Immunoenzyme Techniques; D013974:Thyroxine / Q000097:blood*
D013974:Thyroxine