Large-scale physical mapping within the region 22q12.3-13.1 in meningioma.
The lack of physical mapping data strongly restricts the analysis of the meningioma chromosomal region that was assigned to the bands 22q12.3-qter. Recently, we reported a new marker D22S16 for chromosome 22 that was assigned to the region 22q13-qter by in situ hybridization. Utilizing somatic cell hybrids we now sublocalized the marker D22S16 within the band region 22q12-13.1, thus placing it in the vicinity of the gene for the platelet derived growth factor (PDGFB). A physical map was established for the regions surrounding the PDGFB gene and the D22S16 marker. By means of pulsed-field gel electrophoresis (PFGE) D22S16 and PDGFB were found to be physically linked within 900 kb. We also identified two CpG clusters bordering the PDGFB gene. For the enzyme NotI, a variation of the PDGFB restriction pattern was found between different individuals. PFGE analysis of the two loci (PDFGB and D22S16) failed to identify major rearrangements in meningioma.
University of Saarland, Department of Human Genetics, Homburg/Saar, Germany.
R
R
Herzog
E
E
Gottert
W
W
Henn
K
K
Zang
N
N
Blin
J
J
Trent
E
E
Meese
D000818:Animals; D015139:Blotting, Southern; D002478:Cells, Cultured; D002871:Chromosome Banding; D002892:Chromosomes, Human, Pair 22; D004273:DNA, Neoplasm / Q000235:genetics* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology; D008577:Meningeal Neoplasms / Q000235:genetics* / Q000473:pathology; D008579:Meningioma / Q000235:genetics* / Q000473:pathology; D051379:Mice; D009693:Nucleic Acid Hybridization; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D015183:Restriction Mapping; D012867:Skin / Q000473:pathology
D004273:DNA, Neoplasm; D005819:Genetic Markers; D010982:Platelet-Derived Growth Factor