Simultaneous analysis of the patterns of nuclear DNA and cell protein contents in pancreatic carcinoma with reference to prognosis.
Simultaneous analysis of the patterns of nuclear DNA and cell protein contents was performed in 13 patients with pancreatic carcinoma to evaluate their prognostic significance. The patients were divided into two groups according to clinical outcome. Namely, six died of the primary disease within one year (group A), and six survived longer than 16 months and one died of other cause (liver abscess) at 9 months after operation (group B). The cell suspensions prepared from paraffin-embedded specimens were stained with diamidino-phenylindole and hematoporphyrin for nuclear DNA and cell protein, respectively. Fluorescence intensities of 20 lymphocytes as control and 200 carcinoma cells of each case were measured. The nuclear DNA pattern was mainly diploid or tetraploid in group B. Group A showed aneuploid and polyploid patterns. Furthermore, mean DNA content of group A was significantly higher than that of group B. Cell protein content was widely scattered and higher in group A than group B. Analysis of nuclear DNA ploidy, relative value of DNA and cell protein content may be a reliable tool to predict prognosis of patients with carcinoma of the pancreas.
Department of Surgery, Sendai National Hospital.
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K
Kakizaki
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H
Yamauchi
D000368:Aged; D000369:Aged, 80 and over; D002467:Cell Nucleus / Q000737:chemistry*; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005453:Fluorescence; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis*; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D011379:Prognosis
D004273:DNA, Neoplasm; D009363:Neoplasm Proteins