A pseudogene on the X chromosome for the human trifunctional enzyme MTHFD (methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase).
The human trifunctional folate-dependent protein MTHFD has been mapped to chromosome 14q24 and to the X chromosome was identified by screening an X-chromosome-specific library. Amplification by the polymerase chain reaction (PCR) and sequencing of PCR products indicate that the sequence is an intronless pseudogene.
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Department of Pediatrics, McGill University, Montreal Children's Hospital Research Institute, Montreal, Quebec, Canada.
John
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MacKenzie
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Rozen
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D000619:Aminohydrolases / Q000235:genetics*; D001483:Base Sequence; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005574:Formate-Tetrahydrofolate Ligase / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP) / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011544:Pseudogenes; D014960:X Chromosome
D009097:Multienzyme Complexes; C055342:methylene tetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase; C021836:formyl-methenyl-methylenetetrahydrofolate synthetase; D004247:DNA; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP); D000619:Aminohydrolases; D005574:Formate-Tetrahydrofolate Ligase