Three dimensional reconstruction of human pachytene spermatocyte nuclei of a 17;21 reciprocal translocation carrier: study of XY-autosome relationships.
A study of XY-autosome relationships at the pachytene stage in an infertile 17-21 reciprocal translocation carrier was undertaken by means of three dimensional reconstruction. Synaptonemal complexes and the sex vesicle were analysed on electron microscopic serial sections and the reconstruction was performed on transparent sheets and on a Samba 2000 (Alcatel TITN) image analysis system. All asynapsed segments were entirely included in the sex vesicle, the chromatin fibre of the autosomes and sex chromosomes being tightly intermingled. In one nucleus, the four arms of the quadrivalent were paired, except around the breakpoints where an interstitial asynapsis was observed. In the other nuclei, a terminal asynapsis involving one or two arms of the quadrivalent was found. In the sex vesicle, autosomal asynapsed segments showed the same morphological characteristics as those of X and Y chromosomes. This observation agrees with the hypothesis of the extension of gene inactivation from sex chromosomes to autosomes.
Guichaoua
M R
MR
Laboratoire d'Embryologie et Cytogénétique, Faculté de Médecine, Marseille, France.
de Lanversin
A
A
Cataldo
C
C
Delafontaine
D
D
Alasia
C
C
Fraterno
M
M
Terriou
P
P
Stahl
A
A
Luciani
J M
JM
D002467:Cell Nucleus / Q000648:ultrastructure; D002886:Chromosomes, Human, Pair 17; D002891:Chromosomes, Human, Pair 21; D003198:Computer Simulation; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D011418:Prophase; D013090:Spermatocytes / Q000648:ultrastructure*; D014178:Translocation, Genetic; D014960:X Chromosome; D014998:Y Chromosome