Quantitation of GLUT1 and GLUT4 mRNA using a solution hybridization assay.
The development of a solution hybridization assay for detecting GLUT1 and GLUT4 mRNA is described. The details of this assay are described in which copy RNA is used to quantitate messenger RNA in total RNA samples. This solution hybridization assay is highly specific and reproducible and is significantly more sensitive than Northern blotting. Since GLUT mRNAs can be quantitated in as little as 25 mg tissue, this technique is essential when the supply of tissue is limited. Furthermore, the elimination of gel-based separation techniques allows for mRNA quantitation in several hundred samples within two days following isolation of samples.
Department of Medicine, Medical College of Wisconsin, Milwaukee 53226.
D A
DA
Meier
D
D
Pastorek
R G
RG
James
S R
SR
Hager
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000502:physiology; D007668:Kidney / Q000502:physiology; D008099:Liver / Q000502:physiology; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009132:Muscles / Q000502:physiology; D009928:Organ Specificity; D010957:Plasmids; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000032:analysis
D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins