Isolation and characterization of a new variant of surfactin, the [Val7]surfactin.
Reinvestigation of surfactin, a previously studied peptidolipid surfactant from Bacillus subtilis, by fast-atom-bombardment mass spectrometry and 1H-NMR spectroscopy, as well as by chemical methods, revealed the presence of a closely related second constituent. This new compound, [Val7]surfactin, differs from the known surfactin by the C-terminal amino acid residue which is valine instead of leucine.
Laboratorie de Biochimie Microbienne, Université Claude Bernard Lyon I, Villeurbanne, France.
F
F
Peypoux
J M
JM
Bonmatin
H
H
Labbé
B C
BC
Das
M
M
Ptak
G
G
Michel
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D014644:Genetic Variation; D055666:Lipopeptides; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014633:Valine / Q000737:chemistry*
D001426:Bacterial Proteins; D055666:Lipopeptides; D010456:Peptides, Cyclic; C009365:surfactin peptide; D014633:Valine