Effects of indomethacin on demineralized bone-induced heterotopic ossification in the rat.
Indomethacin inhibits bone formation when treatment is initiated before the implantation of demineralized bone matrix (DBM). For the inhibition of bone induction to occur, indomethacin treatment had to be initiated 6 h or more before implantation of DBM. Initiating the drug treatment at or after the time of DBM implantation had no effects on the amounts of new bone formed. The inhibition by indomethacin is dose related over a range between 0.04 and 4 mg/kg body weight. Recovered day-1 DBM implants, transplanted into indomethacin pre- and posttreated syngeneic rats, formed bone at the same rate as controls did. However, recovered day-1 DBM implants lyophilized before transplantation showed decreased bone formation but significant dystrophic calcification as judged by a lower alkaline phosphatase activity and an elevated calcium content.
Bone and Connective Tissue Biochemistry Research Laboratory, University of Southern California School of Medicine, Orthopaedic Hospital, Los Angeles 90007.
P E
PE
DiCesare
M E
ME
Nimni
L
L
Peng
M
M
Yazdi
D T
DT
Cheung
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015519:Bone Density / Q000187:drug effects / Q000502:physiology*; D001846:Bone Development / Q000187:drug effects; D016025:Bone Transplantation; D002118:Calcium / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D007213:Indomethacin / Q000494:pharmacology*; D009999:Ossification, Heterotopic / Q000503:physiopathology*; D051381:Rats; D011916:Rats, Inbred F344
D000469:Alkaline Phosphatase; D002118:Calcium; D007213:Indomethacin