Detection, localization, and quantitation of HIV-associated antigens in intestinal biopsies from patients with HIV.
This study determined the prevalence, cellular localization, and content of human immunodeficiency virus (HIV)-associated antigens in intestinal mucosa from HIV-infected subjects. Studies were performed in 168 subjects with gastrointestinal, nutritional, or proctologic complaints, and HIV-seronegative controls. The polymerase chain-reaction technique, which detects viral DNA, was used in 20 subjects and was positive in 70%. In situ hybridization studies, using RNA probes, were employed in 48 cases and were positive in 31%. Immunohistologic studies using monoclonal antibodies to HIV p24 antigen were employed in 73 cases and were positive in 67%. Quantitative ELISA assays for tissue p24 content were performed in 168 cases and was positive in 68%. Evidence of HIV was found throughout the intestine and in different disease stages. The quantitative ELISA studies correlated significantly with in situ hybridization, implying a possible association between the presence of viral RNA and protein expression. The authors conclude that HIV is present in intestinal mucosa from most, if not all HIV-infected subjects. The relationship to intestinal disease currently is unclear.
Department of Medicine, St. Luke's-Roosevelt Hospital Center, Columbia University, College of Physicians and Surgeons, New York, NY 10025.
D P
DP
Kotler
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S
Reka
A
A
Borcich
W J
WJ
Cronin
D000911:Antibodies, Monoclonal; D001706:Biopsy; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015488:HIV Antigens / Q000032:analysis* / Q000276:immunology; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000276:immunology / Q000473:pathology; D007422:Intestines / Q000276:immunology* / Q000473:pathology; D008297:Male; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D015347:RNA Probes; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics
D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D015488:HIV Antigens; D015347:RNA Probes; D012367:RNA, Viral