[Lysosomal enzymes and natural killer activity].
The natural killer activity (NKA) of human mononuclear cells and the activity of the lysosomal enzymes of these cells (arylsulfatase, acid phosphatase and beta-glucuronidase) has been studied in norm and under human lung cancer. The mononuclear cells were isolated from peripheral blood of 10 healthy donors and 20 patients with lung cancer of II-III stages. Under the action of mononuclear cells on the target cells (human erythroleukosis cells K-562 labeled with 3H-uridine) the NKA of mononuclear cells of patients was seen to decrease (cytotoxic index = 54.8 +/- 6.4%), in comparison with that of healthy donors (cytotoxic index = 65.1 +/- 4.5%). Simultaneously a decrease in arylsulfatase activity (0.05 +/- 0.01 nmoles/10(6) cells/min) was found in comparison with the control value (0.11 +/- 0.01 nmoles/10(6) cells/min). In 2-3 weeks after the operation the NKA value (cytotoxic index = 50.2 +/- 5.8%) was restored and arylsulfatase activity (0.09 +/- 0.02 nmoles/10(6) cells/min) was increased. There was no correlation between the NKA value and the activities of acid phosphatase and beta-glucuronidase. The parallelism observed between changes in NKA value and arylsulfatase activity may suggest a possible participation of this enzyme in the killing mechanism at the stage of cerebroside sulfate ester degradation of the target cell membrane to initiate the lytic events.
A G
AG
Bulychev
T V
TV
Blinova
D000135:Acid Phosphatase / Q000097:blood; D000368:Aged; D001192:Arylsulfatases / Q000097:blood; D002469:Cell Separation; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D005966:Glucuronidase / Q000097:blood; D006801:Humans; D007694:Killer Cells, Natural / Q000201:enzymology* / Q000276:immunology; D008175:Lung Neoplasms / Q000201:enzymology / Q000276:immunology / Q000601:surgery; D008247:Lysosomes / Q000201:enzymology* / Q000276:immunology; D008875:Middle Aged; D011013:Pneumonectomy; D011184:Postoperative Period
D000135:Acid Phosphatase; D001192:Arylsulfatases; D005966:Glucuronidase
Lizosomnye fermenty i aktivnost' estestvennykh killerov.