A comparative investigation and identification of Leptospira interrogans serogroup icterohaemorrhagiae strains by monoclonal antibody and DNA fingerprint analyses.
The identification of Leptospira interrogans icterohaemorrhagiae strains from a number of Reference Laboratories were confirmed using monoclonal antibody (MoAb) and DNA restriction endonuclease (EcoR1) analyses. With a few exceptions, strain fidelity was demonstrated. Three clinical isolates and one isolate from a rat (Rattus norvegicus) were identified on DNA fragment patterns and found to be similar to the reference strains, icterohaemorrhagiae copenhageni, I. "icterohaemorrhagiae" Ictero I and I. icterohaemorrhagiae RGA.
PHLS Leptospira Reference Laboratory, FAO/WHO Collaborating Centre, County Hospital, Hereford, U.K.
J V
JV
Hookey
M F
MF
Palmer
D000818:Animals; D000911:Antibodies, Monoclonal; D016172:DNA Fingerprinting; D004269:DNA, Bacterial; D007921:Leptospira interrogans / Q000145:classification* / Q000235:genetics / Q000276:immunology; D051381:Rats
D000911:Antibodies, Monoclonal; D004269:DNA, Bacterial