A Saporin-6 cDNA containing a precursor sequence coding for a carboxyl-terminal extension.
Saporin-6 is a single-chain ribosome inactivating protein (RIP) from the seeds and the leaves of Saponaria officinalis (Caryophyllaceae). Here we have identified the COOH-terminal end of mature Saporin-6 and, by cDNA sequencing, the predicted carboxyl-terminal sequence of a leaf Saporin-6 primary translation product. Our data indicate that the characterized cDNA codes for a precursor containing a 22 amino acid carboxyl-terminal extension, not present in mature Saporin-6, that shows similarity to carboxyl-terminal propeptides of vacuolar proteins, suggesting that it may be involved in protein trafficking.
Department of Biotechnology, San Raffaele Research Institute, Milano, Italy.
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Benatti
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Solinas
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Valsasina
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Vitale
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Soria
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D000922:Immunotoxins; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases; D010446:Peptide Fragments / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins
D000922:Immunotoxins; D010446:Peptide Fragments; D010940:Plant Proteins; D011498:Protein Precursors; D054789:Ribosome Inactivating Proteins, Type 1; D004247:DNA; D009699:N-Glycosyl Hydrolases; D000076985:Saporins