[Spectrum and dynamics of free amino acids in bovine ocular tissue and their interaction with pilocarpine. IV. The sclera].
Two-dimensional ascending chromatographic analysis revealed that a 3% aqueous pilocarpine solution dropped into the conjunctival sac of cattle 2.5 hours after the last instillation shifts in a specific qualitative and quantitative way the levels of different free amino acids in the anterior, equatorial and posterior portion of the sclera. This alkaloid in the anterior and posterior part reduces and in the equatorial part of the sclera increases the total levels of free amino acids. The greatest drop in the anterior portion occurred in leucine + isoleucine methionine + valine and glutamine, in the posterior part threonine and leucine + isoleucine. The greatest rise in the equatorial part was observed in glutamine, leucine + isoleucine and taurine.
Katedra zivocísnej fyziológie a etológie Prírodovedeckej fakulty UK, Bratislava.
J
J
Veselovský
L
L
Kovácik
S
S
Rehák
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D005260:Female; D006801:Humans; D010862:Pilocarpine / Q000494:pharmacology*; D012590:Sclera / Q000187:drug effects / Q000378:metabolism*
D000596:Amino Acids; D010862:Pilocarpine
Spektrum a dynamika vol'ných amínokyselín tkanív hovädzieho oka a ich interakcia s pilokarpínom. IV. Sklera.