Colocalization of N-CAM and N-cadherin in avian skeletal myoblasts.
The cell-cell adhesion molecules, N-CAM and N-cadherin, have been shown previously to mediate myoblast interaction during cell fusion accompanying skeletal myogenesis. To study the localization of both molecules in fusion-competent myoblasts, we used antigen-specific primary antibodies and a double-labeling preembedding immuno-electron microscopy technique. Ultrastructural observations and quantitative analysis of the results reveal that N-CAM and N-cadherin frequently colocalize in clusters on the myoblast plasma membrane. The data provide morphological evidence that the two adhesion glycoproteins cooperate in mediating myoblast interaction during myoblast fusion.
Soler
A P
AP
Lankenau Medical Research Center, Wynnewood, Pennsylvania 19096.
Knudsen
K A
KA
D000818:Animals; D015820:Cadherins / Q000032:analysis*; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis*; D002478:Cells, Cultured; D002642:Chick Embryo; D016253:Microscopy, Immunoelectron; D009132:Muscles / Q000737:chemistry* / Q000196:embryology / Q000648:ultrastructure
D015820:Cadherins; D015816:Cell Adhesion Molecules, Neuronal