Changes in the activities of jejunal glucosidases in experimental intestinal tumorigenesis induced by 1,2 dimethylhydrazine in rats fed different diets.
The purpose of the present study was to establish the effect of the carcinogen 1,2 dimethylhydrazine on the activities of the jejunal glucosidases and to assess the possible modifying effect of different diets. Two control groups of Wistar albino rats were used - fed standard pellet diet and fed the same diet + 1,2 dimethylhydrazine treatment. Six experimental groups treated with 1,2 dimethylhydrazine were provided. One of them was fed standard diet, containing 30% of wheaten bran and the other 5 groups received high-lipid diets, containing 30% of different fats. The rats were injected subcutaneously once a week for 12 weeks with 20 mg 1,2 dimethylhydrazine/kg b.m. and left for 12 weeks in order to develop a tumor growth. The activities of 5 glucosidases (lactase, maltase, sucrase, palatinase and cellobiase) were determined in homogenates from jejunal mucosa taken near by the tumors and in homogenates from the tumors themselves. An expressed decrease of the jejunal glucosidase activities near the tumors and in the tumors was established. The animals fed 30% wheaten bran diet did not develop tumorigenesis and showed comparatively slight decrease of the enzyme activities. In general, the high-fat regimens did not exert such a preventive effect.
Institute of Gastroenterology and Nutrition, Bulgarian Medical Academy, Sofia.
G
G
Savov
N
N
Donchev
D019813:1,2-Dimethylhydrazine; D000284:Administration, Oral; D000818:Animals; D050260:Carbohydrate Metabolism; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage; D004127:Dimethylhydrazines; D005959:Glucosidases / Q000378:metabolism*; D006868:Hydrolysis; D007279:Injections, Subcutaneous; D007413:Intestinal Mucosa / Q000378:metabolism; D007580:Jejunal Neoplasms / Q000139:chemically induced / Q000201:enzymology* / Q000517:prevention & control; D007583:Jejunum / Q000201:enzymology*; D043322:Lactase; D008297:Male; D051381:Rats; D013393:Sucrase / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D002273:Carcinogens; D004041:Dietary Fats; D004127:Dimethylhydrazines; D005959:Glucosidases; D043322:Lactase; D000520:alpha-Glucosidases; D001616:beta-Galactosidase; D013393:Sucrase; D019813:1,2-Dimethylhydrazine