High-performance liquid chromatographic separation of human apotransferrin and monoferric and diferric transferrins.
A simple method for the chromatographic separation of the different molecular forms of human transferrin according to their respective iron contents is described. The appropriate conditions were developed with a Mono-S cation-exchange column.
Makino
Y
Y
Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Kawanishi
E
E
D001059:Apoproteins / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D014168:Transferrin / Q000302:isolation & purification*
D001059:Apoproteins; C031139:C-terminal monoferrictransferrin; C031138:N-terminal monoferrictransferrin; D014168:Transferrin; C028284:apotransferrin; C028283:diferric transferrin