Enhanced insulin secretory response to acetylcholine by perifused pancreas of 5-day-old preobese Zucker rats.
The insulin secretion rate in response to different secretagogues and neurotransmittors was studied in perifused pancreas of 5-day-old lean (Fa/Fa) and preobese (fa/fa) Zucker rats. Glucose (16.6 mM) alone or in combination with 20 mM arginine or 5 mM theophylline induced a net stimulation of insulin secretion. This effect was similar in the two groups. By contrast, the stimulatory effect of acetylcholine on glucose-induced insulin secretion was significantly higher in preobese pups than in lean rats. There was also a tendency toward a higher inhibitory effect of norepinephrine on insulin secretion in preobese than in lean rats, but this difference did not reach statistical significance. Together these results demonstrate a normal insulin secretion in response to nutrient secretagogues in preobese fa/fa rats but an enhanced effect of acetylcholine. This latter effect may be related to the changes in the autonomic nervous system activity, which is usually described in obese fa/fa rats.
Laboratoire de Physiopathologie de la Nutrition, CNRS URA 307, Université Paris VII, France.
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D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001120:Arginine / Q000494:pharmacology; D004198:Disease Susceptibility / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D009638:Norepinephrine / Q000494:pharmacology; D009765:Obesity / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D011924:Rats, Zucker / Q000378:metabolism*; D013806:Theophylline / Q000494:pharmacology
D007328:Insulin; D001120:Arginine; D013806:Theophylline; D005947:Glucose; D000109:Acetylcholine; D009638:Norepinephrine