A new strategy to improve a cauliflower mosaic virus vector.
Co-infection of plants with non-overlapping deletion mutants of cauliflower mosaic virus usually leads to the production of the wild-type virus. To prevent this, a pair of mutants with overlapping deletions was constructed. In infected plants both mutant DNAs were stably maintained. Such mutants with overlapping deletions will be used as a vector to overcome the size limitation of genes to be cloned.
Hirochika
H
H
Department of Molecular Biology, National Institute of Agrobiological Resources, Ibaraki, Japan.
Hayashi
K
K
D015139:Blotting, Southern; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004279:DNA, Viral; D004587:Electrophoresis, Agar Gel; D005822:Genetic Vectors; D009029:Mosaic Viruses / Q000235:genetics*; D009154:Mutation; D010944:Plants / Q000235:genetics; D010957:Plasmids
D004274:DNA, Recombinant; D004279:DNA, Viral