Synthetic guinea pig insulin B-chain C-terminal decapeptide: a novel immunogen for generating immunocytochemical-grade antisera.
Antisera to guinea pig insulin are not commonly available, largely because of the short supply and limited immunogenicity of the intact hormone. To overcome these problems we have employed a novel reagent, synthetic guinea pig insulin B-chain C-terminal decapeptide, as a hapten for raising antibodies that react with intact guinea pig insulin. The decapeptide, coupled to bovine serum albumin, was successfully used as an immunogen in rabbits. The resulting anti-serum was employed for immunocytochemical staining of guinea pig insulin in pancreatic sections. The specificity of the staining was verified by both pre-absorption and pre-immune serum controls. The utility of this new antiserum for investigations of guinea pig insulin physiology is discussed.
Division of Endocrinology and Metabolism, Long Island Jewish Medical Center, New Hyde Park, New York 11042.
J
J
Maimon
M
M
Mauro
K C
KC
Gorray
B S
BS
Schneider
D000595:Amino Acid Sequence; D000818:Animals; D006168:Guinea Pigs; D007106:Immune Sera; D007150:Immunohistochemistry; D007328:Insulin / Q000378:metabolism* / Q000502:physiology; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism*
D007106:Immune Sera; D007328:Insulin; D010446:Peptide Fragments