Angiotensin-like immunoreactive cells in the chicken retina.
The cellular distribution of Angiotensin II (ANGII) in the chicken retina was studied by immunocytochemical methods. Three different antibodies directed against ANGII revealed a distinct subpopulation of ANGII-positive amacrine cells. Their cell bodies were located in the innermost rows of the inner nuclear layer (INL). ANGII-positive cell bodies also were found in the ganglion cell layer (GCL). Faint labelling in the centre of the INL was visible, probably related to Müller cell bodies. Single ANGII-positive fibres, each arising from an amacrine cell body, ran to sublayer 3 or 5 of the INL, forming narrow plexi. In addition, small fibres connecting sublayer 3 with sublayer 5 could be observed. ANGII-positive fibres could neither be detected in the optic nerve nor in the outer parts of the retina. Incubation of the tissue in ANGII or angiotensin I (ANGI) dissolved in Ringer solution prior to fixation, increased number and intensity of labelled cells and resulted in better labelling of sublayers 3 and 5 of the inner plexiform layer (IPL). Blockage of the conversion of ANGI to ANGII by adding captopril to the ANGI containing pre-incubation medium reduced the ANGII induced staining enhancement. All three ANGII-antibodies showed corresponding results. These results indicate that a subpopulation of amacrine cells contains angiotensin II in chicken retina. This subpopulation is apparently able to take up ANGII and ANGI, to convert ANGI to ANGII and to store ANGII.
Department of Pathophysiology of Vision and Neuroophthalmology, University Eye Hospital, Tübingen, Germany.
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D000804:Angiotensin II / Q000032:analysis* / Q000276:immunology; D000818:Animals; D002645:Chickens; D007150:Immunohistochemistry; D012160:Retina / Q000737:chemistry* / Q000166:cytology; D012165:Retinal Ganglion Cells / Q000737:chemistry
D000804:Angiotensin II