DNA-protein complexes induced by chromate and other carcinogens.
DNA-protein complexes induced in intact Chinese hamster ovary cells by chromate have been isolated, analyzed, and compared with those induced by cis-platinum, ultraviolet light, and formaldehyde. Actin has been identified as one of the major proteins complexed to DNA by chromate based upon its molecular weight, isoelectric point, positive reaction with an actin polyclonal antibody, and proteolytic mapping. Chromate and cis-platinum both complex proteins of similar molecular weight and isoelectric points, and these complexes can be disrupted by chelating agents and sulfhydryl reducing agents, suggesting that the metal itself is participating in binding rather than having a catalytic or indirect role (i.e., oxygen radicals). In contrast, formaldehyde complexed histones to the DNA, and these complexes were not disrupted by chelating or reducing agents. An antiserum raised to chromate-induced DNA-protein complexes reacted primarily with 97,000 kDa protein that did not silver stain. Slot blots, as well as Western blots, were used to detect formation of p97 DNA crosslinks. This protein was complexed to the DNA by all four agents studied.
Costa
M
M
Institute of Environmental Medicine, New York University Medical Center, NY 10016.
D000818:Animals; D016466:CHO Cells / Q000187:drug effects / Q000378:metabolism; D002273:Carcinogens / Q000633:toxicity; D002840:Chromates / Q000378:metabolism / Q000633:toxicity*; D002945:Cisplatin / Q000633:toxicity; D006224:Cricetinae; D004247:DNA / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D008970:Molecular Weight; D011506:Proteins / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism
D002273:Carcinogens; D002840:Chromates; D011506:Proteins; D004247:DNA; D002945:Cisplatin