Effects of isoflurane on the DNase I activity in an isolated enzyme preparation and on the DNase I-G actin complex.
Effects of isoflurane on the DNase I activity in an isolated enzyme preparation and in the DNase I-globular (G) actin complex were investigated. DNase I, DNase I-G actin complex, and G actin were exposed to various (0.2-4.0 vol%) isoflurane concentrations for 180 min. Thereafter, DNase I activity was determined. DNase I activity was inhibited in relation to time and concentration of isoflurane exposure. At concentrations ranging from 0.2 to 1.0 vol% of isoflurane inactive DNase I was activated in the DNase I-G actin complex. The DNase I inhibitor G actin showed a reduced capability to inhibit DNase I following isoflurane exposure. Albumin can inhibit the DNase I inactivation possibly by competition in the reactions between DNase I/albumin and isoflurane. After exposure to isoflurane the absorption maximum of DNase I was identical with the absorption maximum of heat-denatured DNase I. The results suggest a mechanism by which isoflurane may affect DNA in an indirect way at concentrations to which the patient is exposed during clinical anesthesia.
Reitz
M
M
Department of Physiological Chemistry, University of Mainz, Federal Republic of Germany.
von Pfeil
A
A
Hundt
F
F
Lanz
E
E
D000199:Actins / Q000037:antagonists & inhibitors* / Q000187:drug effects; D000818:Animals; D002021:Buffers; D002417:Cattle; D004249:DNA Damage; D003850:Deoxyribonuclease I / Q000037:antagonists & inhibitors* / Q000187:drug effects; D007530:Isoflurane / Q000494:pharmacology*; D010179:Pancreas / Q000201:enzymology; D011489:Protein Denaturation
D000199:Actins; D002021:Buffers; D007530:Isoflurane; D003850:Deoxyribonuclease I