Comparative study of biosynthetic human growth hormone immunogenicity in growth hormone deficient children.
The immunogenicities of six recombinant human growth hormone (rhGH) preparations, from KABI (A rhGH191 and B rhGH192), Eli Lilly (C), Nordisk (D), Sanofi (E) and Serono (F), used to treat 260 GH-deficient children, have been compared using a common specific and sensitive procedure for antibody determination. For this purpose we developed two immunoassays: a competitive liquid radioimmunoassay using 125I-rhGH, and an immunometric solid enzymoimmunoassay in which the rhGHs were immobilized. Blood samples were collected from the GH-deficient children before treatment and after 3, 6, 9, 12, 18 and 24 months of therapy. Human GH antibodies were detected in children treated with 3 of the 6 rhGH preparations. Seven percent of the patients treated with hormone A, 14% with hormone B and 22% with hormone C formed antibodies against the respective rhGH. Differences in capacity and affinity of the hGH antibodies were observed between these anti-GH-positive groups. They could be divided into 2 groups according to their immunopotency. One group (7, 14 and 6% of the patients treated with hormones A, B and C, respectively) developed anti-hGH antibodies with very low binding capacities (30-100 fmol/ml). The other group (16% of the patients treated with hormone C) developed IgG-type antibodies to hGH with higher binding capacities (200-1,200 fmol/ml) and a measurable binding affinity (Ka = 10(8) M-1). These hGH antibodies partially inhibited the binding of labeled GH to its specific liver membrane receptor. However, because of their low titer, they did not inhibit growth in the treated children.(ABSTRACT TRUNCATED AT 250 WORDS)
Rougeot
C
C
U207 INSERM, URIA, Institut Pasteur, Paris, France.
Marchand
P
P
Dray
F
F
Girard
F
F
Job
J C
JC
Pierson
M
M
Ponte
C
C
Rochiccioli
P
P
Rappaport
R
R
D000293:Adolescent; D000906:Antibodies / Q000097:blood*; D000941:Antigens / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D013006:Growth Hormone / Q000172:deficiency / Q000276:immunology*; D006801:Humans; D007700:Kinetics; D008297:Male; D011863:Radioimmunoassay; D011994:Recombinant Proteins / Q000276:immunology*
D000906:Antibodies; D000941:Antigens; D011994:Recombinant Proteins; D013006:Growth Hormone