Plant cardiac glycosides and digoxin Fab antibody.
The potential application of the Digoxin Fab antibody (Wellcome Digibind) in the clinical management of plant poisoning was investigated. The cardiac glycoside contents of various Australian plants were studied using immunoassay techniques. The cross-reactions of the Fab antibody and two digoxin assay antibodies against extracts of these plants were also studied. Results obtained indicated that the Digibind antibody cross-reacted with a wide range of glycosides contained in Australian plants and therefore could be of use in the treatment of life-threatening plant poisoning.
Department of Clinical Chemistry, Prince Charles Hospital, Brisbane, Queensland, Australia.
K
K
Cheung
R
R
Urech
L
L
Taylor
P
P
Duffy
D
D
Radford
D000328:Adult; D001315:Australia; D002301:Cardiac Glycosides / Q000032:analysis* / Q000276:immunology; D002648:Child; D003429:Cross Reactions; D004077:Digoxin / Q000276:immunology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000627:therapeutic use; D010936:Plant Extracts / Q000276:immunology; D010939:Plant Poisoning / Q000175:diagnosis* / Q000628:therapy; D010947:Plants, Toxic / Q000737:chemistry*; D011863:Radioimmunoassay
D002301:Cardiac Glycosides; D007140:Immunoglobulin Fab Fragments; D010936:Plant Extracts; C050199:digoxin antibodies Fab fragments; D004077:Digoxin