The immune response to anti-idiotype antibodies bearing an internal image epitope of tetanus toxin/toxoid. I. Induction of the humoral immune response.
Primary immune responses to tetanus toxoid (TT) and primary and secondary immune responses to a rabbit TT internal image bearing anti-idiotype antibody (Ab2 beta 1) inoculated in Freund's complete adjuvant (FCA), saline (SAL) or syntex adjuvant formulation vehicle (SAF) via the intraperitoneal or subcutaneous route, were examined in mice. High anti-TT antibody (Ab3) titres are reported although the titre and persistence of the antibody response varied according to the adjuvant used in the priming and challenge inocula of Ab2 beta 1. Mouse Ab3 antibodies were elicited in mice inoculated with rabbit Ab2 beta 1 antibodies which in turn were elicited by an inoculum of mouse monoclonal anti-TT Ab1 antibody. Ab3 was shown to be identical to Ab1 by immunoblot analysis. Primary and secondary immune responses elicited by rabbit Ab2 beta 1 antibody protected mice against a lethal dose of tetanus toxin.
Poskitt
D C
DC
Commonwealth Serum Laboratories, Research and Development Division, Melbourne, Australia.
Turnbull
S
S
Macdonald
L
L
Jean-François
M J
MJ
Yasmeen
D
D
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis* / Q000276:immunology; D000937:Antigen-Antibody Reactions; D015153:Blotting, Western; D002846:Chromatography, Affinity; D005620:Freund's Adjuvant / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D051379:Mice; D008807:Mice, Inbred BALB C; D012965:Sodium Chloride; D013744:Tetanus Toxin / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology*; D014611:Vaccination
D000888:Antibodies, Anti-Idiotypic; D013744:Tetanus Toxin; D013745:Tetanus Toxoid; D012965:Sodium Chloride; D005620:Freund's Adjuvant