X/Y translocations resulting from recombination between homologous sequences on Xp and Yq.
Several regions of sequence homology between the human X and Y chromosomes have been identified. These segments are thought to represent areas of these chromosomes that have engaged in meiotic recombination in relatively recent evolutionary times. Normally, the X and Y chromosomes pair during meiosis and exchange DNA only within the pseudoautosomal region at the distal short arms of both chromosomes. However, it has been suggested that aberrant recombination involving other segments of high homology could be responsible for the production of X/Y translocations. We have studied four X/Y translocation patients using molecular probes detecting homologous sequences on X and Y chromosomes. In one translocation the breakpoints have been isolated and sequenced. The mapping data are consistent with the hypothesis that X/Y translocations arise by homologous recombination. The sequencing data from one translocation demonstrate this directly.
Howard Hughes Medical Institute, University of California, Los Angeles.
P H
PH
Yen
S P
SP
Tsai
S L
SL
Wenger
M W
MW
Steele
T K
TK
Mohandas
L J
LJ
Shapiro
D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005819:Genetic Markers; D015698:Genomic Library; D006801:Humans; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D014178:Translocation, Genetic; D014960:X Chromosome; D014998:Y Chromosome
D005819:Genetic Markers; D004247:DNA