Classical conditioning-induced changes in low-molecular-weight GTP-binding proteins in rabbit hippocampus.
Classical conditioning of Hermissenda, involving paired light-rotation events, results in a 30-35% decrease in the levels of a 20-kDa G protein (cp20). To test whether a similar protein exists in vertebrates, rabbits were trained to associate a tone with periorbital electrical stimulation and G proteins were analyzed by photoaffinity labeling with [alpha-32P]GTP-azidoanilide. A 20-kDa G protein similar to cp20 decreased by 36% in the hippocampus of rabbits subjected to paired tone and electrical stimulation, but not in unpaired controls. Learning-specific decreases were also found in the amount of ras protein.
Nelson
T J
TJ
National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892.
Sanchez-Andres
J V
JV
Schreurs
B G
BG
Alkon
D L
DL
D000161:Acoustic Stimulation; D000345:Affinity Labels; D000818:Animals; D001386:Azides / Q000378:metabolism; D003214:Conditioning, Classical / Q000502:physiology*; D004558:Electric Stimulation; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D020690:GTPase-Activating Proteins; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D009994:Osmolar Concentration; D011506:Proteins / Q000378:metabolism; D011817:Rabbits; D020703:ras GTPase-Activating Proteins
D000345:Affinity Labels; D001386:Azides; D020690:GTPase-Activating Proteins; D011506:Proteins; D020703:ras GTPase-Activating Proteins; C031907:GTP gamma-4-azidoanilide; D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins