Behavior in immunoblotting of dimeric and monomeric forms of nonspecific cross-reacting antigen (NCA-50) from normal lung.
The dimeric and monomeric forms of NCA-50 from normal lung showed differences in binding to nitrocellulose in an immunoblotting procedure. The NCA dimer showed weaker binding, it passed through nitrocellulose and was more easily washed out from the blot. The NCA monomer was bound strongly and was detected with higher sensitivity following immunoblotting.
Department of Immunochemistry, Polish Academy of Sciences, Wrocław.
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Krop-Watorek
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Sedlaczek
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Lisowska
D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic / Q000737:chemistry / Q000302:isolation & purification*; D000951:Antigens, Neoplasm; D001345:Autoradiography; D015153:Blotting, Western / Q000379:methods*; D002272:Carcinoembryonic Antigen / Q000302:isolation & purification; D015815:Cell Adhesion Molecules; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D006801:Humans; D008168:Lung / Q000276:immunology; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic; D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; D015815:Cell Adhesion Molecules; D008562:Membrane Glycoproteins; D003101:Collodion