Use of DNA fingerprinting in paternity analysis of closely-related Exmoor ponies.
DNA fingerprinting techniques were used to try to resolve the parentage of an Exmoor pony foal. Three young Exmoor ponies, one female and two males, shared a paddock and the female subsequently became pregnant. The two possible sires were three-quarter siblings and were also half-siblings to the dam. Southern hybridisation of Exmoor pony DNA with human mini-satellite probes resolved the disputed parentage in spite of the fact that there was a 70 per cent band share between the individuals involved. Colt M6 was 2.06 times more likely to be the father than an uncle, and Colt M3 was 477 times more likely to be an uncle than the father.
ICI Pharmaceuticals, Cheshire, UK.
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B
Hopkins
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FM
O'Connell
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J
Hopkins
D000818:Animals; D015139:Blotting, Southern; D004247:DNA / Q000032:analysis; D016172:DNA Fingerprinting; D015342:DNA Probes; D004276:DNA, Satellite; D005260:Female; D006736:Horses / Q000235:genetics*; D008297:Male; D009693:Nucleic Acid Hybridization; D010334:Paternity; D010375:Pedigree; D011110:Polymorphism, Genetic
D015342:DNA Probes; D004276:DNA, Satellite; D004247:DNA