Direct radioactive labeling of unpurified PCR products using Klenow fragment.
We describe a method for rapid radioactive labeling of PCR product. The method, employing the Klenow fragment of DNA polymerase I, consumes little product, requires no product purification and takes under 30 minutes.
Department of Medicine, University of Maryland, Baltimore.
T K
TK
McDaniel
Y
Y
Huang
J
J
Yin
S W
SW
Needleman
S J
SJ
Meltzer
D004256:DNA Polymerase I; D010761:Phosphorus Radioisotopes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid
D010761:Phosphorus Radioisotopes; D004256:DNA Polymerase I