[Detection of antibodies against Chlamydia in swine by an immunofluorescent test and an enzyme immunoassay].
Chlamydial IgG-antibodies were detected in 130 of 2050 porcine sera (6.3%) using an indirect immunofluorescence assay (IFA). Antibody titers varied between 1:8 and 1:16 in 87 sera (67%) and reached titers from 1:32 to 1:128 in 43 sera (33%). An ELISA closely related to the IFA-technique was developed, which confirmed the IFA-positive and IFA-negative results in 93% and 86% respectively. A significant higher percentage of positive IFA-titers greater than or equal to 1:32 was obtained from sows with abortions and infertility as well as from store pigs with pneumonia than from healthy pigs (p less than 0.5).
Institut für Mikrobiologie und Tierseuchen der Tierärztlichen Hochschule Hannover.
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Wittenbrink
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D002690:Chlamydia Infections / Q000276:immunology / Q000662:veterinary*; D002691:Chlamydophila psittaci / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D013552:Swine; D013553:Swine Diseases / Q000276:immunology*
D000907:Antibodies, Bacterial
Nachweis von Antikörpern gegen Chlamydien beim Schwein mit Hilfe eines Immunofluoreszenz- und eines Enzymimmunotests.