Recirculating, retrograde heart perfusion according to Langendorff as a tool in the evaluation of drug-induced cardiomyopathy: effects of a high lipid diet.
A recirculating, retrograde heart perfusion according to Langendorff is described as a method for the evaluation of cardiomyopathy as an untoward side effect of a high lipid diet (addition of 10% and 25% corn oil to rat maintenance feed) in female rats. The use of glucose (5 mM) or palmitate (0.5 mM/0.1 mM BSA) as substrates during a 2-h perfusion period, and their effects on heart metabolism of control-, LL- and HL-diet fed animals were evaluated. Substrate uptake, LDH release and adenine nucleotides, creatine phosphate, creatine, lactate, pyruvate, glucose-6-phosphate, glycogen, triglyceride and phospholipid content were determined in heart tissue.
Bachmann
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Institute of Toxicology, Swiss Federal Institute of Technology.
Weber
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E
D000818:Animals; D009202:Cardiomyopathies / Q000209:etiology*; D003402:Creatine Kinase / Q000378:metabolism; D004041:Dietary Fats / Q000009:adverse effects*; D004734:Energy Metabolism; D005260:Female; D006003:Glycogen / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D010477:Perfusion; D010725:Phosphocreatine / Q000032:analysis; D011773:Pyruvates / Q000032:analysis; D019289:Pyruvic Acid; D051381:Rats
D004041:Dietary Fats; D011773:Pyruvates; D010725:Phosphocreatine; D019289:Pyruvic Acid; D006003:Glycogen; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase