Utilization and metabolism of [U-14C]4' galactosyllactose (O-beta-D-galactopyranosyl-(1----4)-O-beta-D-galactopyranosyl-(1----4)- D-glucopyranose) in rats.
O-beta-D-Galactopyranosyl-(1----4)-O-beta-D-galactopyranosyl- (1----4)-D-glucopyranose (designated as 4'GL) are produced from lactose with Cryptococcus laurentii OKN-4. Excretion and metabolism of 4'GL in rats were examined using a radioisotope technique. [U-14c]4'GL was synthesized from [U-14C]lactose by Cryptococcus laurentii OKN-4. The 14CO2 in expired air was counted after oral administration of [U-14C]4'GL or [U-14C]lactose in conventional rats, rats treated with antibiotics and germ-free rats. The rate of 14CO2 excretion from conventional rats given [U-14C]4'GL was slower than that from those administered [U-14C]lactose. When [U-14C]4'GL was orally administered to rats given antibiotics, there was a 2-h delay in 14CO2 excretion, as compared to conventional rats. In germ-free rats, total excretion of 14CO2 from [U-14C]-4'GL decreased to about one-third of that of conventional rats during a 24-h period. Radioactivities in the serum, liver, and carcass of the [U-14C]4'GL oral administration group were lower than those of the [U-14C]lactose oral administration group. Radioactivities in the feces and urine however, were higher in [U-14C]4'GL group than in [U-14C]lactose group.
Ohtsuka
K
K
Nissin Sugar Mfg. Co. Ltd., Koutou-ku, Tokyo, Japan.
Tsuji
K
K
Nakagawa
Y
Y
Ueda
H
H
Ozawa
O
O
Uchida
T
T
Ichikawa
T
T
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002245:Carbon Dioxide / Q000032:analysis; D002250:Carbon Radioisotopes; D004734:Energy Metabolism; D005243:Feces / Q000737:chemistry; D007408:Intestinal Absorption; D007785:Lactose / Q000008:administration & dosage; D008297:Male; D008657:Metabolic Clearance Rate; D051381:Rats; D011919:Rats, Inbred Strains; D014312:Trisaccharides / Q000008:administration & dosage / Q000493:pharmacokinetics*
D000900:Anti-Bacterial Agents; D002250:Carbon Radioisotopes; D014312:Trisaccharides; D002245:Carbon Dioxide; C047819:4-O-(4-O-alpha-galactopyranosyl-beta-galactopyranosyl)glucopyranose; D007785:Lactose