IL-2 production by B cells stimulated with a specific antigen.
The ability of a specific antigen (Ag) to stimulate B cells to produce IL-2 was examined with a murine B lymphoma line, A20-HL, which expressed surface IgM specific for trinitrophenyl (TNP). The culture supernatant of A20-HL cells stimulated with TNP3.9-ovalbumin (-OVA) or anti-IgM goat IgG contained an activity which supported the proliferation of an IL-2-dependent T cell line, CTLL-2. Neither TNP3.9-OVA nor anti-IgM antibody stimulated the parent line, A20.2J, which did not bear TNP-specific sIg, whereas anti-mouse Ig rabbit IgG F(ab)2 did stimulate both A20-HL cells and A20.2J cells. The active material in the culture supernatant was identified as IL-2 based on the experiments in which the activity was inhibited by anti-IL-2 mAb, and IL-2 mRNA was expressed in A20-HL cells stimulated with TNP3.9-OVA or anti-IgM antibody. These results support the conclusion that a specific Ag can stimulate A20-HL cells to produce IL-2. For IL-2 production, TNP receptors on A20-HL cells have to be appropriately cross-linked, inasmuch as either TNP3.9-OVA or TNP6.7-OVA was much more effective than TNP1.2-OVA and TNP22.9-OVA in the induction of IL-2 production by A20-HL cells.
Department of Allergology, Institute of Medical Science, University of Tokyo, Japan.
T
T
Kakiuchi
T
T
Tamura
Y
Y
Gyotoku
H
H
Nariuchi
D000941:Antigens / Q000276:immunology*; D001402:B-Lymphocytes / Q000378:metabolism*; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D010047:Ovalbumin / Q000276:immunology; D011961:Receptors, Fc / Q000502:physiology; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology; D014407:Tumor Cells, Cultured
D000941:Antigens; D007376:Interleukin-2; D011961:Receptors, Fc; D015375:Receptors, Interleukin-2; C035311:immunoglobulin M receptor; C038600:trinitrophenyl-ovalbumin; D010047:Ovalbumin