Direct in vitro and in vivo monitoring of destruxins metabolism in insects using internal surface reversed-phase high-performance liquid chromatography. I. Behaviour of E destruxin in locusts.
High-performance liquid chromatography with internal surface reversed-phase packing provides an analytical tool for studying the in vitro and in vivo metabolism of A and E destruxins in the haemolymph and various organs of male adults of Locusta migratoria. A slight amount of injected E destruxin is shown to be hydrated into E-diol destruxin in the haemolymph. The rest of the toxin is recovered unchanged in the fat-body, pericardial tissues and Malpighian tubules, and some further E-diol destruxin formation occurs in these organs. Because E-diol destruxin is only weakly toxic, this appears to be a detoxication process.
Cherton
J C
JC
Laboratoire de Chimie Organique Structurale, CNRS URA 455, Université P et M Curie, Paris, France.
Lange
C
C
Mulheim
C
C
Païs
M
M
Cassier
P
P
Vey
A
A
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D047630:Depsipeptides; D004784:Environmental Monitoring; D005216:Fat Body / Q000378:metabolism; D006110:Grasshoppers / Q000378:metabolism*; D006458:Hemolymph / Q000378:metabolism; D008658:Inactivation, Metabolic; D008297:Male; D008317:Malpighian Tubules / Q000378:metabolism; D010456:Peptides, Cyclic / Q000378:metabolism*; D010496:Pericardium / Q000378:metabolism
D047630:Depsipeptides; D010456:Peptides, Cyclic; C040423:destruxin A