[HIV infection of CD4-CD8+ T-cell line derived from patients with HAM].
This studies have attempted human immunodeficiency virus (HIV) infection in four CD4+ or CD8+ T-cell lines derived from HTLV-I associated myelopathy virus (HAM) patients. Not only CD4+ cell line but also CD8+ cell line could be infected with HIV and CD4+ cell line showed a higher susceptibility than CD8+ cell line on HIV infection. HIV antigen in early stage after HIV inoculation was detected by using enzyme-linked immunosorbent assay (ELISA) rather than indirect immunofluorescence assay (IFA). HTLV-I producing CD4+ and CD8+ cell lines became to express two viral antigens (HTLV-I and HIV) after HIV inoculation. The results indicated that CD4-CD8+ T-cell line from patient with HAM can be infected with HIV. So that, we have found that other epitopes except for CD4 antigen may be associated with HIV infection.
Shoji
H
H
AIDS Research Center, National Institute of Health, Tokyo.
Nakanaga
K
K
Yoshihara
N
N
D015704:CD4 Antigens / Q000378:metabolism*; D016827:CD8 Antigens / Q000378:metabolism*; D002460:Cell Line; D015488:HIV Antigens / Q000378:metabolism; D015497:HIV-1; D006801:Humans; D015493:Paraparesis, Tropical Spastic / Q000276:immunology / Q000473:pathology*; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology*
D015704:CD4 Antigens; D016827:CD8 Antigens; D015488:HIV Antigens