Potential application of dot-immunobinding assay as a rapid diagnostic test in tuberculous meningitis.
A simple dot-immunobinding assay (Dot-Iba) in nitrocellulose paper was developed for the detection of specific IgG antibody to Mycobacterium tuberculosis antigen 5 and mycobacterial antigen in cerebrospinal fluid of patients with tuberculous meningitis (TBM). The assay gave 77.1% sensitivity for the detection of IgG antibody to M. tuberculosis antigen 5 and 48.6% sensitivity for the detection of mycobacterial antigen in patients with TBM.
Department of Pathology, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Trivandrum, India.
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Radhakrishnan
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Mathai
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D008297:Male; D008875:Middle Aged; D014390:Tuberculosis, Meningeal / Q000175:diagnosis*