Characterization of Rrh4273I, a restriction-modification system of Rhodococcus rhodochrous ATCC 4273 (Nocardia corallina) which recognizes the same sequence as the Streptomyces albus G SalI restriction-modification system.
Rhodococcus rhodochrous ATCC 4275 (Nocardia corallina) has a restriction-modification system with the same recognition sequence, methylation site and cleavage site as the SalI restriction-modification system. Both the restriction endonuclease and the DNA-methyltransferase (DNA-MTase) have been partially purified and characterized. The nuclease has requirements of activity similar to SalI, and a native Mr of about 46,000. The DNA-MTase is a protein with an Mr of about 67,000. No DNA homology was detected between the cloned salI restriction-modification genes of Streptomyces albus and R. rhodochrous chromosomal DNA.
Area de Microbiologia, Departamento de Biologia Funcional, Facultad de Medicina, Universidad de Oviedo, Spain.
M J
MJ
Yebra
I S
IS
Novella
C
C
Barbés
J F
JF
Aparicio
C G
CG
Martin
C
C
Hardisson
J
J
Sánchez
D001483:Base Sequence; D004269:DNA, Bacterial / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D008745:Methylation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012240:Rhodococcus / Q000201:enzymology* / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000302:isolation & purification / Q000378:metabolism*
D004269:DNA, Bacterial; C059634:DNA modification methylase SalGI; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D015252:Deoxyribonucleases, Type II Site-Specific; C029792:GTCGAC-specific type II deoxyribonucleases