Chymotrypsin treatment increases the activity of Clostridium perfringens enterotoxin.
Chymotrypsin treatment of the enterotoxin from Clostridium perfringens results in the loss of 36 amino acids from the N-terminus and 3 amino acids from the C-terminus. This processing results in a 3.2 fold increase in activity on Vero cells, which is close to what had been found for trypsin treatment following the loss of 25 amino acids from the N-terminus.
Department of Food Hygiene, Norwegian College of Veterinary Medicine, Oslo.
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Granum
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Richardson
D000595:Amino Acid Sequence; D000818:Animals; D002918:Chymotrypsin / Q000378:metabolism*; D003016:Clostridium perfringens; D004768:Enterotoxins / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D014709:Vero Cells
D004768:Enterotoxins; C035598:enterotoxin, Clostridium; D002918:Chymotrypsin