Gas chromatographic-mass spectrometric determination of two new antimycotic agents, 1-[(5-chloro-2-benzofuranyl)(2-chlorophenyl)methyl]-1H-imidazole and 1-[(5-bromo-2-benzofuranyl)phenylmethyl]-1H-imidazole, in rabbit plasma following topical administration: a preliminary comparison with bifonazole.
A method for the analysis of the antimycotic drugs 1-[(5-chloro-2-benzofuranyl)(2-chlorophenyl)methyl]-1H-imidazole, 1-[(5-bromo-2-benzofuranyl)phenylmethyl]-1H-imidazole and bifonazole in rabbit plasma, employing gas chromatography-mass spectrometry with selected-ion monitoring, was developed. The procedure involved single-step purification of the biological matrix via liquid-liquid extraction on Extrelut columns and use of a carrier substance to minimize the negative effects of adsorption sites during the gas chromatographic process. The limits of detection ranged from 0.1 to 1.4 ng/ml, starting from a 200-microliter sample. The method was applied to a preliminary evaluation of percutaneous absorption of both drugs in the rabbit after a single administration, in comparison with bifonazole.
Research Division Analytical Department, A. Menarini Industrie Farmaceutiche Riunite S.R.L., Florence, Italy.
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Mannucci
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Perico
D000287:Administration, Topical; D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage / Q000097:blood*; D001572:Benzofurans / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D007093:Imidazoles / Q000008:administration & dosage / Q000097:blood*; D007477:Ions; D011817:Rabbits
D000935:Antifungal Agents; D001572:Benzofurans; D007093:Imidazoles; D007477:Ions; C071094:1-((5-bromo-2-benzofuranyl)phenylmethyl)-1H-imidazole; C071093:1-((5-chloro-2-benzofuranyl)(2-chlorophenyl)methyl)-1H-imidazole; C036596:bifonazole