Isolation and characterisation of a bovine cDNA encoding eukaryotic initiation factor 2 alpha.
Two cDNA clones have been isolated, from a bovine lymphosarcoma library, that encode the alpha-subunit of eukaryotic initiation factor 2 (eIF-2 alpha). The predicted 315 amino acid sequence showed more than 99% amino acid identity with rat and human eIF-2 alpha. Galactose-regulated expression of a full length bovine eIF-2 alpha cDNA in yeast resulted in the synthesis of a polypeptide of the predicted molecular mass (36 kDa). Furthermore, the expressed polypeptide cross-reacted with an antibody raised against rabbit eIF-2 alpha confirming the identity of the cDNA.
Biological Laboratory, University of Kent, Canterbury, U.K.
S R
SR
Green
J
J
Fullekrug
K
K
Sauer
M F
MF
Tuite
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001483:Base Sequence; D002417:Cattle; D003429:Cross Reactions; D004247:DNA / Q000302:isolation & purification*; D015852:Eukaryotic Initiation Factor-2 / Q000235:genetics* / Q000276:immunology; D015870:Gene Expression; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism; D008969:Molecular Sequence Data; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid
D000906:Antibodies; D015852:Eukaryotic Initiation Factor-2; D004247:DNA