Building ruggedness into HPLC quality control procedures.
HPLC methods for protein and peptide analysis are excellent in terms of resolution, selectivity and recovery. They are, however, complex procedures involving multiple parameters including instrumentation, columns and mobile phases that may lead to inter- or intra-laboratory deviations. The factors that contribute to differences in retention, selectivity and sensitivity are discussed, followed by suggestions for eliminating or compensating for the differences.
Gooding
K M
KM
SynChrom, Inc., Lafayette, IN 47902.
Schmuck
M N
MN
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D011786:Quality Control; D015203:Reproducibility of Results; D013696:Temperature