Characterization of crystals of an intact monoclonal antibody for canine lymphoma.
A monoclonal antibody of the subclass IgG2a specific for canine lymphoma cells has been crystallized by vapor diffusion from polyethylene glycol 8000. the crystals, which occasionally measure nearly a millimeter on edge, have been examined by X-ray diffraction. The crystals are of triclinic space group P1 with unit cell parameters of a = 66.39 A, b = 77.34 A, c = 101.42 A, alpha = 87.60 degrees, beta = 92.55 degrees, gamma = 97.54 degrees and cell volume of V = 4.84 x 10(5) A3. There is one entire antibody molecule as the asymmetric unit of the crystals. Three-dimensional X-ray diffraction data have been collected to 2.8 A resolution and a self rotation function calculation shows a pronounced peak indicating at least an approximate non-crystallographic dyad axis.
Larson
S
S
Department of Biochemistry, University of California, Riverside 92521.
Day
J
J
Greenwood
A
A
Skaletsky
E
E
McPherson
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000648:ultrastructure*; D000912:Antibodies, Neoplasm / Q000276:immunology / Q000648:ultrastructure*; D003460:Crystallization; D004285:Dogs; D007074:Immunoglobulin G / Q000276:immunology / Q000648:ultrastructure*; D008223:Lymphoma / Q000276:immunology*; D014961:X-Ray Diffraction
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D007074:Immunoglobulin G