High-performance liquid chromatographic method for the simultaneous determination of R-(-)- and S-(+)-hexobarbital in rat plasma.
The enantiospecific determination of R- and S-hexobarbital in rat plasma is described. The method involves liquid-liquid extraction of racemic hexobarbital from plasma, separation of the underivatized enantiomers by high-performance liquid chromatography on an alpha 1-acid glycoprotein column and ultraviolet detection. The mobile phase consists of a phosphate buffer (pH 5.4) containing 0.4% 2-propanol as organic modifier. An alpha 1-acid glycoprotein guard column is used to increase the lifetime of the analytical column. Heptabarbital is the achiral internal standard. With detection limits of ca. 0.05 microgram/ml for both R- and S-hexobarbital, the assay is suitable for pharmacokinetic studies of the enantiomers in rats.
Heymans Institute of Pharmacology, University of Ghent Medical School, Belgium.
A M
AM
Vermeulen
M T
MT
Rosseel
F M
FM
Belpaire
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D006591:Hexobarbital / Q000097:blood*; D007202:Indicators and Reagents; D007275:Injections, Intravenous; D009961:Orosomucoid; D051381:Rats; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D007202:Indicators and Reagents; D009961:Orosomucoid; D006591:Hexobarbital