Genetic studies on a major merozoite surface antigen of the malaria parasite of rodents, Plasmodium chabaudi.
Diversity in a major merozoite surface antigen (MSA-1) of Plasmodium chabaudi has been examined using a panel of monoclonal antibodies (MoAbs). The antigen was found to be different in each of twelve cloned isolates, as shown by its reactivity with the MoAbs in immunofluorescence tests. In genetic crossing experiments, the diverse forms of this antigen were shown to be determined by allelic variation of a single gene. Recombination occurred between the MSA-1 gene, a second blood stage antigen and enzyme markers.
McLean
A P
AP
Institute of Cell, Animal and Population Biology, Edinburgh, Scotland, UK.
Lainson
F A
FA
Sharkey
A M
AM
Walliker
D
D
D000818:Animals; D000911:Antibodies, Monoclonal; D000940:Antigenic Variation; D000953:Antigens, Protozoan / Q000235:genetics* / Q000276:immunology; D000954:Antigens, Surface / Q000235:genetics* / Q000276:immunology; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D051379:Mice; D008807:Mice, Inbred BALB C; D016788:Plasmodium chabaudi / Q000235:genetics*; D011995:Recombination, Genetic
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000954:Antigens, Surface