The effects of short-term application of a combination of platelet-derived and insulin-like growth factors on periodontal wound healing.
Polypeptide growth factors are a class of potent natural biologic mediators which regulate many of the activities of wound healing including cell proliferation, migration, and metabolism. Platelet-derived growth factor (PDGF) and insulin-like growth factor-I (IGF-I) have been shown to regulate DNA and protein synthesis in bone cells in vitro and to interact synergistically to enhance soft tissue wound healing in vivo. We have hypothesized that the combination of PDGF and IGF-I may, therefore, enhance regeneration of both the soft and hard tissue components of the periodontium. To test this hypothesis we performed conventional periodontal surgery on all 4 quadrants of the mouth of 13 beagle dogs with naturally occurring periodontal disease. Following flap reflection, degranulation, and root planing, all premolar teeth in 2 quadrants of each dog received a combination of 3 micrograms of recombinant PDGF-B and IGF-I in a methylcellulose gel, while the premolar teeth in the contralateral quadrants received the gel alone. Teeth in 4 additional animals also received 125I-PDGF or 125I-IGF-I in the treated sites. The clearance rate of the 125I-labeled protein, changes in local bone metabolism, and amount of new bone and cementum with inserting collagen fibers were measured. The clearance studies revealed that the half-life of the factors at the site of application was 3.0 hours for IGF-I and to 4.2 hours for PDGF-B. Greater than 96% of the radio-labeled proteins was cleared by 96 hours and no radioactivity was detected 2 weeks after application. There was a significant (P less than 0.01) 2-fold increase in uptake of the bone-seeking radiopharmaceutical Technetium 99-MDP at 2 and 4 weeks in growth factor treated sites compared to controls, indicating that there was increased metabolic activity within the bone at these sites. Computer-aided histologic analyses of biopsies obtained at 2 and 5 weeks post-operatively revealed a significant (P less than 0.01), 5 to 10 fold increase in new bone and cementum in PDGF-B/IGF-I treated sites at both time points compared to controls receiving the placebo gel. The height and total area of new bone continued to increase from 2 to 5 weeks. The new bone underwent a normal maturation process as judged by histologic appearance. A physiologic periodontal ligament space was also formed between the new bone and new cementum. There was no increase in ankylosis in the treated sites.(ABSTRACT TRUNCATED AT 400 WORDS)
Lynch
S E
SE
Institute of Molecular Biology, Inc., Boston, MA.
de Castilla
G R
GR
Williams
R C
RC
Kiritsy
C P
CP
Howell
T H
TH
Reddy
M S
MS
Antoniades
H N
HN
D016301:Alveolar Bone Loss / Q000188:drug therapy / Q000473:pathology / Q000601:surgery; D000539:Alveolar Process / Q000378:metabolism / Q000473:pathology; D000818:Animals; D003238:Connective Tissue / Q000473:pathology; D004285:Dogs; D004338:Drug Combinations; D005260:Female; D005891:Gingivitis / Q000188:drug therapy / Q000473:pathology / Q000601:surgery; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D007457:Iodine Radioisotopes; D008657:Metabolic Clearance Rate; D010006:Osteoblasts / Q000473:pathology; D010011:Osteocytes / Q000473:pathology; D010510:Periodontal Diseases / Q000188:drug therapy* / Q000473:pathology / Q000601:surgery; D010513:Periodontal Ligament / Q000473:pathology; D010982:Platelet-Derived Growth Factor / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D011994:Recombinant Proteins; D012038:Regeneration / Q000187:drug effects; D013524:Surgical Flaps; D013669:Technetium Tc 99m Medronate; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
D004338:Drug Combinations; D007457:Iodine Radioisotopes; D010982:Platelet-Derived Growth Factor; D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I; D013669:Technetium Tc 99m Medronate