A novel cis-acting element that controls transcription of human immunodeficiency virus type 1 DNA, depending on cell type.
cis-acting elements for the transcription of human immunodeficiency virus type 1 DNA were analyzed in cell-free transcription and DNA transfection assays. Besides previously identified cis elements, a region adjacent to the enhancer element was found to regulate transcription in both assays. Loss of this region caused 4.3- and 1.6-fold transcription inhibition in a transfection assay with a T-cell line, MOLT-4, and a monocyte line, U937, respectively, whereas the same region appeared to function negatively with other T-cell lines, MT-4 and Jurkat. These results suggest that this novel cis element regulates the transcription of proviral DNA in a cell-type-specific manner.
Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.
Y
Y
Nakanishi
Y
Y
Masamune
N
N
Kobayashi
D001483:Base Sequence; D002460:Cell Line; D002474:Cell-Free System; D004279:DNA, Viral / Q000235:genetics*; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014162:Transfection
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides