Parvalbumin in the cardiac muscle of normal and haemoglobin-myoglobin-free antarctic fish.
Three parvalbumin isotypes were isolated from the white muscle of haemoglobin-myoglobin-free antarctic fish Channichthys rhinoceratus. Antibodies against the parvalbumin mixture were raised in rabbits and used for discovery, quantitation and isolation by affinity chromatography of parvalbumin in the cardiac muscle of three antarctic fish species: Channichthys rhinoceratus, Champsocephalus gunnari and Notothenia neglecta. The cardiac muscle of these species contains parvalbumin in concentration close to 1 mumol per kg wet weight.
Laforet
C
C
Laboratoire de Biochimie, Université de Liège, Belgium.
Feller
G
G
Narinx
E
E
Gerday
C
C
D000818:Animals; D000864:Antarctic Regions; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes / Q000502:physiology*; D006454:Hemoglobins / Q000032:analysis; D007158:Immunologic Techniques; D009132:Muscles / Q000737:chemistry*; D009206:Myocardium / Q000737:chemistry*; D009211:Myoglobin / Q000032:analysis; D010320:Parvalbumins / Q000032:analysis* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D006454:Hemoglobins; D009211:Myoglobin; D010320:Parvalbumins