Enhanced protein breakdown after eccentric exercise in young and older men.
The effects of eccentric exercise on whole body protein metabolism were compared in five young untrained [age 24 +/- 1 yr, maximal O2 uptake (VO2max) = 49 +/- 6 ml.kg-1.min-1] and five older untrained men (age 61 +/- 1 yr, VO2max = 34 +/- 2 ml.kg-1.min-1). They performed 45 min of eccentric exercise on a cycle ergometer at a power output equivalent to 80% VO2max (182 +/- 18 W). Beginning 5 days before exercise and continuing for at least 10 days after exercise, they consumed a eucaloric diet providing 1.5 g.kg-1.day-1 of protein. Leucine metabolism in the fed state was measured before, immediately after, and 10 days after exercise, with intravenous L-[1-13C]leucine as a tracer (0.115 mumol.kg-1.min-1). Leucine flux increased 9% immediately after exercise (P less than 0.011) and remained elevated 10 days later, with no effect of age. Leucine oxidation increased 19% immediately after exercise and remained 15% above baseline 10 days after exercise (P less than 0.0001), with no effect of age. In the young men, urinary excretion of 3-methylhistidine per gram of creatinine did not increase until 10 days postexercise (P less than 0.05), but in the older men, it increased 5 days after exercise and remained high through 10 days postexercise (P less than 0.05), averaging 37% higher than in the young men. These data suggest that eccentric exercise produces a similar increase in whole body protein breakdown in older and young men, but myofibrillar proteolysis may contribute more to whole body protein breakdown in the older group.
Human Physiology Laboratory, US Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, Massachusetts 02111.
R A
RA
Fielding
C N
CN
Meredith
K P
KP
O'Reilly
W R
WR
Frontera
J G
JG
Cannon
W J
WJ
Evans
D000328:Adult; D000375:Aging / Q000378:metabolism*; D002151:Calorimetry; D002245:Carbon Dioxide / Q000378:metabolism; D003402:Creatine Kinase / Q000378:metabolism; D015444:Exercise / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008297:Male; D008762:Methylhistidines / Q000652:urine; D008875:Middle Aged; D009584:Nitrogen / Q000652:urine; D010101:Oxygen Consumption / Q000502:physiology; D011506:Proteins / Q000378:metabolism*
D008762:Methylhistidines; D011506:Proteins; D002245:Carbon Dioxide; D003402:Creatine Kinase; D007930:Leucine; C028118:3-methylhistidine; D009584:Nitrogen