Effect of glucose and branched chain amino acid (BCAA) infusion on onset of liver regeneration and plasma amino acid pattern in partially hepatectomized rats.
Immediately after 70% hepatectomy, rats were infused via the jugular vein with one of the following solutions: saline, 3% or 8% glucose solution, 3% BCAA solution, 8% Nutramin (17% BCAA) or 8% Nutramin enriched with BCAA (47% BCAA). Rats were killed 18, 21, 24 and 30 h after partial hepatectomy. The course of liver regeneration was assessed from incorporation of labeled thymidine into DNA and from hepatocyte mitotic activity. After partial hepatectomy a decrease of glycemia and increase of plasma phenylalanine concentrations were observed. Glucose infusion had a pronounced inhibiting effect on the onset of liver regeneration. The decreased incorporation of labeled thymidine into DNA and the decreased hepatocyte mitotic activity in glucose-infused rats were associated with higher plasma concentrations of tyrosine and phenylalanine. Parenteral administration of 3% BCAA solution and 8% Nutramin enriched with BCAA had a favourable effect on the onset of liver regeneration after partial hepatectomy compared with glucose- and Nutramin-infused groups. In BCAA-infused rats elevated levels of BCAA and decreased concentrations of tyrosine and phenylalanine were noted.
Department of Physiology, Medical School, Charles University, Hradec Králové, Czechoslovakia.
M
M
Holecek
J
J
Simek
V
V
Palicka
Z
Z
Zadák
D000596:Amino Acids / Q000097:blood*; D000597:Amino Acids, Branched-Chain / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology*; D006498:Hepatectomy; D007262:Infusions, Intravenous; D008099:Liver / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D008115:Liver Regeneration / Q000187:drug effects*; D010649:Phenylalanine / Q000097:blood; D051381:Rats; D011919:Rats, Inbred Strains; D013936:Thymidine / Q000378:metabolism; D014443:Tyrosine / Q000097:blood
D000596:Amino Acids; D000597:Amino Acids, Branched-Chain; D014443:Tyrosine; D010649:Phenylalanine; D004247:DNA; D005947:Glucose; D013936:Thymidine