L-alpha-phosphatidylcholine-induced stimulation of PGI2 production in canine gallbladders following hypovolemic shock and Escherichia coli sepsis.
In vitro production of PGI2 in canine gallbladders subjected to hypovolemic shock and Escherichia coli sepsis was studied to determine whether a precursor above arachidonic acid in the cyclooxyenase cascade might be operative in the production of prostacyclin, which, in turn, may play a role in the pathogenesis of acute acalculous cholecystitis (AC). L-alpha-phosphatidylcholine (LaP), an arachidonic acid precursor, was used as the test agent. LaP did not stimulate PGI2 production from either gallbladder surface in the hypovelimic animals or the mucosa of the septic shock group. However, it did stimulate PGI2 production from the SS serosa compared with controls, 1375 +/- 432 versus 633 +/- 198 pg/cm2/min (P less than .05). In conclusion, lack of stimulation of PGI2 in the hypovolemic model suggests that PGI2 does not play a role in AC. Alternatively, it may play a role in preventing this disease process in septic shock. This study demonstrates the use of precursors of arachidonic acid and the cyclooxygenase pathway as active participants in the production of PGI2, although it is unclear whether the prostacyclin produced helps prevent AC in septic shock.
Hoover
E L
EL
Department of Surgery, Meharry Medical College, Nashville, Tennessee.
Harrison
B S
BS
Williams
W L
WL
Adams
C Z
CZ
Weaver
W L
WL
D000208:Acute Disease; D000818:Animals; D002764:Cholecystitis / Q000209:etiology*; D004285:Dogs; D011464:Epoprostenol / Q000032:analysis / Q000096:biosynthesis*; D004927:Escherichia coli Infections / Q000150:complications / Q000503:physiopathology*; D005260:Female; D005704:Gallbladder / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D010713:Phosphatidylcholines / Q000494:pharmacology; D012769:Shock / Q000150:complications / Q000503:physiopathology*; D012772:Shock, Septic / Q000150:complications / Q000503:physiopathology*
D010713:Phosphatidylcholines; D011464:Epoprostenol