Introduction of BAPTA into intact rat submandibular acini inhibits mucin secretion in response to cholinergic and beta-adrenergic agonists.
Incorporation of the calcium chelator BAPTA into isolated, intact rat submandibular acini by hypotonic swelling, resulted in complete inhibition of carbamylcholine, noradrenaline and isoproterenol stimulation of mucin secretion. No effects of intracellular BAPTA on cell viability or beta-adrenergic stimulation of cyclic AMP formation were observed. The data are the first to demonstrate that calcium is necessary for beta-adrenergic stimulation of secretion and suggest that Ca2+ provides a common link in the triggering of exocytosis.
Mills
C L
CL
Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff, UK.
Dormer
R L
RL
McPherson
M A
MA
D000818:Animals; D002217:Carbachol / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004533:Egtazic Acid / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D005944:Glucosamine / Q000378:metabolism; D007038:Hypotonic Solutions; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D009077:Mucins / Q000096:biosynthesis / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013363:Submandibular Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D007038:Hypotonic Solutions; D009077:Mucins; D004533:Egtazic Acid; D002217:Carbachol; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D007545:Isoproterenol; D005944:Glucosamine; D009638:Norepinephrine