NMDA induces protein kinase C translocation in hippocampal slices of immature rat brain.
N-Methyl-D-aspartate (NMDA)-induced translocation of protein kinase C (PKC) from cytosol to membrane fractions was examined by the methods of [3H]phorbol 12,13-dibutyrate binding and western blotting in rat hippocampal slices. NMDA and L-glutamate induced translocation of PKC from cytosol to membrane fractions in immature rat hippocampal slices, but not in mature ones. The NMDA-induced translocation of PKC was dependent on Ca2+. It was inhibited by the NMDA receptor antagonists, 2-amino-5-phosphonovaleric acid and ketamine, but not by Mg2+ and Zn2+. These results suggest that stimulation of NMDA receptors enhances Ca2+ influx and thereby induces translocation of PKC in immature rat hippocampus.
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
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Etoh
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Baba
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Iwata
D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D005591:Chemical Fractionation; D003600:Cytosol / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D015151:Immunoblotting; D066298:In Vitro Techniques; D016202:N-Methylaspartate / Q000494:pharmacology*; D015240:Phorbol 12,13-Dibutyrate / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats
D015240:Phorbol 12,13-Dibutyrate; D016202:N-Methylaspartate; D011493:Protein Kinase C