Lectin histochemistry of mouse vagina during the estrous cycle.
Estrous cycle-related histochemical changes in the vaginal epithelium of sexually mature female mice were studied with 30 fluorescein isothiocyanate (FITC)-labeled lectins. On the basis of the staining pattern the lectins were divided into five groups: I, seventeen lectins that reacted with mucinous surface layer of proestrus. This group comprised two subgroups: Ia, seven lectins that reacted exclusively with the mucinous layer, and Ib, ten lectins that reacted with mucinous cells and the underlying squamous epithelium of proestrus; II, two lectins that reacted with squamous epithelium of proestrus only but were unreactive with mucinous cells; III, three lectins that reacted in a phase-specific manner with squamous epithelium; IV, six lectins that showed increased luminal surface reactivity in diestrus and/or metestrus; and V, eleven lectins that were unreactive with vaginal epithelium. These data indicate that the cyclic changes in the morphology of the vaginal epithelium are accompanied by distinct lectin reactivity patterns.
Department of Pathology and Cell Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107.
H
H
Vrcić
B
B
Horvat
I
I
Damjanov
D000818:Animals; D002853:Chromatography, Liquid; D004848:Epithelium / Q000378:metabolism; D004971:Estrus; D005260:Female; D006651:Histocytochemistry; D037102:Lectins / Q000378:metabolism*; D051379:Mice; D009077:Mucins / Q000378:metabolism; D014621:Vagina / Q000378:metabolism*
D037102:Lectins; D009077:Mucins