Serum IgG from CBA/K1Jms-lprcg/lprcg mice induces interleukin 3 in an interleukin-3-dependent cell line--possible correlation with lymphadenopathy.
Serum IgG of CBA-K1Jms-lprcg/lprcg (lprcg/lprcg) mice with spontaneous systemic lymphadenopathy supported the proliferation of an IL-3-dependent cell line, FDC-P2/185-4. The lprcg/lprcg IgG induced IL-3 synthesis in FDC-P2/185-4 cells, and cells grew by an autocrine mechanism. There was virtually no time lag between the appearance of lymphadenopathy and an increase of IL-3-inducing activity in the sera. We have previously shown that serum IgG from other autoimmune mice with lymphadenopathy, MRL/Mp-lpr/lpr(MRL/lpr) and C3H/HeJ-gld/gld(C3H/gld), also induces IL-3 synthesis and cell growth in FDC-P2/185-4 cells. Furthermore, neither F1 (lprcg/+) mice between lprcg/lprcg and CBA(-)+/+ nor those (lpr-gld) between C3H-lpr/lpr and C3H/gld showed such IL-3-inducing activity, while those (lprcg-gld) between lprcg/lprcg and C3H/gld showed activity much lower than that of their parental strains but significantly higher than that of normal CBA(-)+/+ mice. This result is consistent with the incidence and degree of lymphadenopathy in these F1 mice. Our results suggest that expression of IgG(s) with cytokine-inducing activity might be controlled by these mutant genes, lpr, gld, and lprcg, and might be related to lymphadenopathy in these mice.
Department of Orthopedics, Yamanashi Medical College, Japan.
E
E
Sato
M
M
Iwamoto
T
T
Sakihama
Y
Y
Yokoyama
Y
Y
Hamada
A
A
Matsuzawa
N
N
Akamatsu
Y
Y
Nakajima
K
K
Tasaka
D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology*; D002455:Cell Division; D002460:Cell Line; D005260:Female; D007074:Immunoglobulin G / Q000502:physiology*; D007377:Interleukin-3 / Q000502:physiology*; D008232:Lymphoproliferative Disorders / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA
D007074:Immunoglobulin G; D007377:Interleukin-3