Late phase increase of thrombin-like proteinase, protein, leukocytes in bronchoalveolar lavage (BAL) fluid and chemiluminescence of BAL after ovalbumin aerosol inhalation by actively immunized rats.
A thrombin-like proteinase (THROLP) was detected colorimetrically as the main proteolytic activity (PROA) in bronchoalveolar lavage fluid (BALF) 4 and 24 h after ovalbumin aerosol (OVA) challenge of actively immunized rats. Other proinflammatory parameters increased also significantly in BALF, like chemiluminescence of leukocytes (1 h), protein and cell number (24 h after challenge). In parallel, increased bronchoconstrictions against 5-HT aerosols are detected 24 h post OVA challenge. THROLP is an indicator for plasma leakage, activation of the clotting reaction and the protracted inflammation in the airways, which induced bronchial hyperreactivity.
Linssen
M
M
Medical Research, Boehringer Mannheim GmbH, FRG.
Lemmermann
C
C
Lipponer
L
L
Wilhelms
O H
OH
D000280:Administration, Inhalation; D000336:Aerosols; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000166:cytology; D010450:Endopeptidases / Q000032:analysis*; D007114:Immunization; D008163:Luminescent Measurements; D010047:Ovalbumin / Q000276:immunology*; D011506:Proteins / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000494:pharmacology; D013917:Thrombin / Q000032:analysis*
D000336:Aerosols; D011506:Proteins; D012701:Serotonin; D010047:Ovalbumin; D010450:Endopeptidases; D013917:Thrombin