Effect of gossypol on kinetics of mouse liver alcohol and aldehyde dehydrogenase.
1. Gossypol, an antifertility ingredient of the cotton plant, altered specific activity of mouse liver alcohol dehydrogenase (L-ADH) and subcellular aldehyde dehydrogenase (L-ALDH) in mice of both sexes. 2. Intraperitoneal injection of a single gossypol dose, 50 mg/kg, inhibited both male and female L-ADH and cytoplasmic L-ALDH from saline controls 21 hr after drug treatment. 3. Gossypol inhibited female but not male mouse mitochondrial L-ALDH isoenzymes. 4. Gossypol-produced enzyme inhibition was determined as noncompetitive. 5. The results suggest gender-dependent sensitivity of mitochondrial L-ALDH to the gossypol inhibition. A toxic metabolic interaction between ethanol and gossypol has been indicated which suggests the contraindication of alcoholic beverages during gossypol use.
Messiha
F S
FS
Texas Tech University Health Sciences Center, School of Medicine, Lubbock 79430.
D000426:Alcohol Dehydrogenase / Q000037:antagonists & inhibitors*; D000444:Aldehyde Dehydrogenase / Q000037:antagonists & inhibitors*; D000818:Animals; D003593:Cytoplasm / Q000201:enzymology; D005260:Female; D006072:Gossypol / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051379:Mice; D008930:Mitochondria, Liver / Q000201:enzymology; D012737:Sex Factors
D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase; D006072:Gossypol