Biosynthetic capacity of the human lens upon aging.
Lipid and protein biosynthesis were investigated in organ culture of human lenses from subjects ranging from 8 to 95 years old. A marked increase of protein and cholesterol content of the human lens with age was found. No significant linear correlation of age with either cholesterol or fatty acid synthesis was observed. Moreover, there are no indications that either synthesis declines with increasing age of the subject. Notwithstanding the large variety in age of the subjects, cholesterol and fatty acid synthesis of the individual lenses were correlated with each other in a positive linear way. Non-cataractous lenses as old as 80 years revealed a normal protein synthesis pattern as determined by incorporation of [35S]methionine into protein and analysis by 2D-gel electrophoresis. In addition, they maintained a high protein biosynthetic capacity.
TNO Institute of Aging and Vascular Research (IVVO-TNO), Gaubius Laboratory, Leiden, The Netherlands.
A C
AC
de Vries
M A
MA
Vermeer
A L
AL
Hendriks
H
H
Bloemendal
L H
LH
Cohen
D000085:Acetates / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D002784:Cholesterol / Q000096:biosynthesis; D003459:Crystallins / Q000096:biosynthesis; D015180:Electrophoresis, Gel, Two-Dimensional; D005227:Fatty Acids / Q000096:biosynthesis; D006801:Humans; D007908:Lens, Crystalline / Q000378:metabolism*; D008875:Middle Aged; D009924:Organ Culture Techniques
D000085:Acetates; D003459:Crystallins; D005227:Fatty Acids; D002784:Cholesterol