The mechanical and electrical effects of rhinoceros viper (Bitis nasicornis) venom on the isolated perfused guinea pig heart and atrial preparations.
The mechanical and electrical effects of the venom of Bitis nasicornis were studied on the guinea-pig Langendorff and left atrial myocardium preparations. While Langendorff preparations were treated with individual doses of 0.1, 0.6 and 1.4 mg, isolated left atria were treated using concentrations of 2.0, 20 and 200 micrograms/ml of venom in the perfusion solution. In the Langendorff preparation, transient increases in left ventricular systolic pressure (LVSP) and heart rate (HR) were seen after 0.1 mg of venom. When 0.6 mg of venom was given, the increases were followed by decreases, while 1.4 mg doses simply induced decreases in LVSP and HR. After both 0.6 and 1.4 mg doses the decreases were accompanied by increases in left ventricular diastolic pressure. In addition to these mechanical effects, transient increases in HR with atrio-ventricular blocks, ventricular extrasystoles and tachycardia were observed after each dose. In the left atrium the 2 micrograms/ml venom concentration produced an increase, followed by a decrease, in the maximum tension developed, which was only seen to decrease with higher concentrations of 20 and 200 micrograms/ml of venom. A dose dependent significant reduction in the action potential duration was observed for the doses of 0.6 and 1.4 mg in the ventricle and for all three concentrations in the atrium.
Dipartimento di Biologia Animale, Università di Torino, Italy.
G
G
Alloatti
D
D
Gattullo
N A
NA
Marsh
P
P
Pagliaro
P
P
Vono
D000200:Action Potentials; D000818:Animals; D016275:Atrial Function; D003326:Coronary Circulation; D006168:Guinea Pigs; D006321:Heart / Q000502:physiology*; D006327:Heart Block / Q000209:etiology; D006339:Heart Rate; D066298:In Vitro Techniques; D009200:Myocardial Contraction; D011899:Ranitidine / Q000494:pharmacology; D014757:Viper Venoms / Q000633:toxicity*
D014757:Viper Venoms; D011899:Ranitidine