Involvement of wild-type p53 in pre-B-cell differentiation in vitro.
Wild-type p53 protein is a growth modulator whose inactivation has been found to be a key event in malignant transformation. Reconstitution of wild-type p53 in the p53-nonproducer, Abelson murine leukemia virus-transformed pre-B-cell line L12 gave rise to stably growing clones. Wild-type p53-producer derived cell lines exhibit an altered cell cycle, however. More cells with an extended G0/G1 phase were found than in the p53-nonproducer parental cell line. Furthermore, when injected into syngeneic mice, these cells induced a lower incidence of tumors and these tumors were less aggressive. Analysis of immunoglobulin expression revealed that wild-type p53 induced the expression of cytoplasmic immunoglobulin mu heavy chain. In addition, these derived cells lines exhibited increased levels of a B-cell-specific surface marker, B220. These results suggest that wild-type p53 may function as a cell differentiation factor that can induce development of pre-B cells into a more advanced stage in the pathway of B-cell maturation. In these pre-B cells, wild-type p53 may induce cell differentiation without terminal growth arrest of the cell population.
Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
G
G
Shaulsky
N
N
Goldfinger
A
A
Peled
V
V
Rotter
D000011:Abelson murine leukemia virus / Q000235:genetics; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D001854:Bone Marrow Cells; D002453:Cell Cycle; D002454:Cell Differentiation; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D015870:Gene Expression; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000032:analysis*; D007148:Immunoglobulin mu-Chains / Q000032:analysis*; D051379:Mice; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000235:genetics / Q000502:physiology*
D000954:Antigens, Surface; D007143:Immunoglobulin Heavy Chains; D007148:Immunoglobulin mu-Chains; D016159:Tumor Suppressor Protein p53