In situ analysis of proteolipid protein gene transcripts during persistent Theiler's virus infection.
SJL/J mice inoculated intracranially with the DA strain of Theiler's virus exhibit a persistent demyelinating disease of the central nervous system. To investigate the effect of persistent infection of oligodendrocytes on the expression of myelin genes, we analyzed the level of PLP mRNA in infected as well as uninfected oligodendrocytes. This study was performed at the single-cell level using the simultaneous detection of viral antigens by immunocytochemistry and PLP mRNAs by in situ hybridization with 35S-labeled oligonucleotide probes. Our data indicate that viral infection of oligodendrocytes reduces the level of PLP mRNA by about 80%.
Unité Virus Lents, UA 1157 CNRS, Institut Pasteur, Paris, France.
S
S
Ozden
C
C
Aubert
D
D
Gonzalez-Dunia
M
M
Brahic
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D002490:Central Nervous System / Q000382:microbiology; D004279:DNA, Viral / Q000235:genetics / Q000276:immunology; D004769:Enterovirus Infections / Q000235:genetics* / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D004680:Maus Elberfeld virus / Q000235:genetics* / Q000302:isolation & purification; D051379:Mice; D009186:Myelin Sheath / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D009836:Oligodendroglia / Q000737:chemistry / Q000382:microbiology; D015345:Oligonucleotide Probes; D011510:Proteolipids / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D013462:Sulfur Radioisotopes; D014158:Transcription, Genetic / Q000235:genetics*
D000956:Antigens, Viral; D004279:DNA, Viral; D015345:Oligonucleotide Probes; D011510:Proteolipids; D012333:RNA, Messenger; D012367:RNA, Viral; D013462:Sulfur Radioisotopes