Complete amino acid sequence of endo-beta-N-acetylglucosaminidase from Flavobacterium sp.
The complete amino acid sequence of endo-beta-N-acetylglucosaminidase from Flavobacterium sp. has been determined by analysis of peptides after cleavage with lysyl endopeptidase, pepsin and chymotrypsin. The protein consists of a single polypeptide chain consisting of 267 amino acid residues and a molecular mass of 27972 Da. The sequence of Flavobacterium endo-beta-N-acetylglucosaminidase is very close to that of the Streptomyces enzyme (endo-H), having 60% similarity and very similar hydropathy profiles. Similarities were also found between Flavobacterium endo-beta-N-acetylglucosaminidase and chitinases from Bacillus circulans, Serratia marcescens and Phaseolus vulgaris.
Takegawa
K
K
Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan.
Mikami
B
B
Iwahara
S
S
Morita
Y
Y
Yamamoto
K
K
Tochikura
T
T
D000595:Amino Acid Sequence; D002918:Chymotrypsin / Q000378:metabolism; D005417:Flavobacterium / Q000201:enzymology*; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D010434:Pepsin A / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases / Q000378:metabolism
D010446:Peptide Fragments; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D012697:Serine Endopeptidases; D002918:Chymotrypsin; C036813:lysyl endopeptidase; D010434:Pepsin A