Production of human monoclonal antibodies in dialysis tubing.
Human x mouse hybridoma cells were grown in dialysis tubing (DT) to obtain large quantities of human monoclonal antibodies (MAb). Hybridoma cells were grown inside the DT, which was placed in a tissue culture flask containing medium. The medium inside the DT was supplemented with different additives which may be selected depending on the intended use of the MAb. About 10-50 times higher concentrations of immunoglobulin (Ig) were obtained after cultivation in DT compared to conventional tissue culture (CTC) for 2 days. The purity of the MAb was high which facilitated further antibody purification. Production of human MAb in DT proved to be excellent for evaluation studies in laboratory scale. It does not require expensive equipment and several hybridomas can be grown simultaneously.
Sjögren-Jansson
E
E
Department of Clinical Virology, University of Göteborg, Sweden.
Ohlin
M
M
Borrebaeck
C A
CA
Jeansson
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002455:Cell Division; D003470:Culture Media; D003956:Dialysis / Q000295:instrumentation*; D006801:Humans; D006825:Hybridomas / Q000166:cytology / Q000276:immunology*; D007158:Immunologic Techniques; D051379:Mice
D000911:Antibodies, Monoclonal; D003470:Culture Media