The effects of heparin cofactor II-derived chemotaxins on neutrophil actin conformation and cyclic AMP levels.
The serine proteinase inhibitor heparin cofactor II (HC) can be cleaved by polymorphonuclear leukocyte (PMN) elastase (LE) to yield potent chemotactic activity for PMN and monocytes. In contrast to the bacterially-derived chemotaxin formyl-Met-Leu-Phe (fMLP), the HC-derived chemotaxin does not stimulate PMN degranulation or oxidative burst activity. We compared the effects of HC-derived chemotaxins to the effects of fMLP on PMN actin conformation and on the cAMP levels. Both the HC chemotaxins and fMLP rapidly induced an increase in F-actin which was similar in magnitude and time-course. However, in contrast to fMLP, HC-derived chemotaxins did not elevate cAMP levels. HC-derived chemotaxins may be useful probes of chemotactic responses, since they do not have the mixed biological activities of fMLP.
Department of Pathology, University of North Carolina School of Medicine, Chapel Hill.
M
M
Hoffman
K A
KA
Faulkner
M A
MA
Iannone
F C
FC
Church
D000199:Actins / Q000032:analysis / Q000737:chemistry*; D002630:Chemotactic Factors / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte; D015844:Heparin Cofactor II / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D019272:Leukocyte Elastase; D008027:Light; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000737:chemistry* / Q000187:drug effects / Q000502:physiology; D010196:Pancreatic Elastase / Q000378:metabolism; D011487:Protein Conformation / Q000187:drug effects; D012542:Scattering, Radiation
D000199:Actins; D002630:Chemotactic Factors; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015844:Heparin Cofactor II; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase