Isolation and primary structure of glucagon from the endocrine pancreas of Thunnus obesus.
Glucagon has been isolated from the endocrine pancreas of a tunid, Thunnus obesus. The primary structure of the glucagon molecule was established as H S E G T F S N D Y S K Y L E T R R A Q D F V Q W L K N S. The sequence is identical to those of sculpin and flounder glucagon and glucagon II from anglerfish. It also shows high homology to the mammalian hormone (76%). The mass determined by fast-atom bombardment (3508) was consistent with the proposed structure. Immunological properties of the tuna glucagon were analyzed by radioimmunoassay, showing a high degree of cross-reactivity with the 30K antibody.
Departament de Bioquímica i Fisiologia, Facultat de Biologia, Universitat de Barcelona, Spain.
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I
Navarro
J
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Gutiérrez
J
J
Caixach
J
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Rivera
J
J
Planas
D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005399:Fishes / Q000502:physiology; D005934:Glucagon / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D007515:Islets of Langerhans / Q000502:physiology*; D008969:Molecular Sequence Data; D011863:Radioimmunoassay; D012689:Sequence Homology, Nucleic Acid; D014413:Tuna / Q000502:physiology*
D005934:Glucagon