Phenylarsine oxide induces the cyclosporin A-sensitive membrane permeability transition in rat liver mitochondria.
This paper reports an investigation on the effects of the hydrophobic, bifunctional SH group reagent phenylarsine oxide (PhAsO) on mitochondrial membrane permeability. We show that PhAsO is a potent inducer of the mitochondrial permeability transition in a process which is sensitive to both the oxygen radical scavanger BHT and to cyclosporin A. The PhAsO-induced permeability transition is stimulated by Ca2+ but takes place also in the presence of EGTA in a process that maintains its sensitivity to BHT and cyclosporin A. Our findings suggest that, at variance from other known inducers of the permeability transition, PhAsO reacts directly with functional SH groups that are inaccessible to hydrophilic reagents in the absence of Ca2+.
C.N.R. Unit for the Study of Physiology of Mitochondria, University of Padova Medical School, Italy.
E
E
Lenartowicz
P
P
Bernardi
G F
GF
Azzone
D000818:Animals; D001152:Arsenicals / Q000494:pharmacology*; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D016572:Cyclosporine / Q000494:pharmacology*; D007425:Intracellular Membranes; D007700:Kinetics; D008564:Membrane Potentials; D008930:Mitochondria, Liver / Q000378:metabolism*; D010539:Permeability / Q000187:drug effects; D051381:Rats
D001152:Arsenicals; C029341:oxophenylarsine; D002084:Butylated Hydroxytoluene; D016572:Cyclosporine; D002118:Calcium