Insulin sensitivity and liver insulin receptor structure in ducks from two genera.
Insulin sensitivity and liver insulin receptor structure were studied in 5-wk-old ducks from two genera (Muscovy and Pekin). In the fasting state, both duck types were equally resistant to exogenous insulin compared with chicken. Despite the low potency of duck insulin, the number of insulin receptors was lower in Muscovy duck and similar in Pekin duck and chicken liver membranes. After 125I-insulin cross-linking, the size of the alpha-subunit of the receptors from the three species was 135,000. Wheat germ agglutinin-purified receptors from the three species were contaminated by an active and unusual adenosinetriphosphatase (ATPase) contaminant (highest activity in Muscovy duck). Sequential purification of solubilized receptor from both duck types on lentil and then wheat germ agglutinin lectins led to a fraction of receptors very poor in ATPase activity that exhibited a beta-subunit size (95,000) and tyrosine kinase activity similar to those of ATPase-free chicken insulin receptors. Therefore the ducks from the two genera exhibit an alpha-beta-structure for liver insulin receptors and a clear difference in the number of liver insulin receptors. Their sensitivity to insulin is, however, similarly decreased compared with chicken.
Station de Recherches Avicoles, Institut National de la Recherche Agronomique, Nouzilly, France.
T
T
Constans
B
B
Chevalier
M
M
Derouet
J
J
Simon
D000818:Animals; D002645:Chickens; D003432:Cross-Linking Reagents; D004372:Ducks / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D007328:Insulin / Q000097:blood / Q000378:metabolism / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism*; D008566:Membranes / Q000378:metabolism; D010766:Phosphorylation; D011972:Receptor, Insulin / Q000737:chemistry* / Q000302:isolation & purification; D012995:Solubility
D003432:Cross-Linking Reagents; D007328:Insulin; D011972:Receptor, Insulin