8-37h-CGRP antagonizes actions of amylin on carbohydrate metabolism in vitro and in vivo.
8-37hCGRP is a potent antagonist at calcitonin gene-related peptide (CGRP) liver membrane receptors; it also blocks vasodilator actions of CGRP and the related peptide, amylin, in intact rats. We now report that 8-37hCGRP antagonizes amylin inhibition of insulin-stimulated labelled glucose uptake into isolated rat soleus muscle, and inhibits amylin-evoked elevation of plasma lactate and glucose in fasted anaesthetized rats. The different relative potencies of the 3 ligands (amylin, CGRP, 8-37hCGRP) on the liver receptor and on the soleus muscle point to distinct CGRP receptors in liver membranes and amylin receptors on muscle cells.
Wang
M W
MW
Physiology Department, Amylin Corporation, San Diego, California 92121.
Young
A A
AA
Rink
T J
TJ
Cooper
G J
GJ
D000595:Amino Acid Sequence; D000682:Amyloid / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D000758:Anesthesia; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015740:Calcitonin Gene-Related Peptide / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000097:blood; D006801:Humans; D058228:Islet Amyloid Polypeptide; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D008297:Male; D008969:Molecular Sequence Data; D009132:Muscles / Q000187:drug effects; D010446:Peptide Fragments / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D000682:Amyloid; D001786:Blood Glucose; D002241:Carbohydrates; D058228:Islet Amyloid Polypeptide; D007773:Lactates; D010446:Peptide Fragments; C058636:calcitonin gene-related peptide (8-37); D019344:Lactic Acid; D015740:Calcitonin Gene-Related Peptide