cDNA cloning of a human androgen-induced mRNA exhibiting an early and protein synthesis-independent induction.
The detailed mechanism of action of androgens remains unknown. We have used an androgen-dependent human prostate cancer cell line and a subtractive cDNA hybridisation strategy to enrich for androgen-dependent sequences. This yielded a cDNA clone which exhibits the characteristics of a primary trans-activated target for androgens. This androgen-regulated gene encodes a polyadenylated 4.5 kb mRNA which is induced 30-50-fold within 3 h of treatment with 10 nM dihydrotestosterone. The induction does not require continued protein synthesis as it is maintained in the presence of protein synthesis inhibitors.
Department of Clinical Biochemistry, University of Cambridge, Addenbrookes Hospital, UK.
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Colletta
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Kealey
D000728:Androgens / Q000502:physiology*; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000302:isolation & purification; D003609:Dactinomycin / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D007700:Kinetics; D008297:Male; D011471:Prostatic Neoplasms; D011691:Puromycin / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D014407:Tumor Cells, Cultured
D000728:Androgens; D012333:RNA, Messenger; D003609:Dactinomycin; D011691:Puromycin; D004247:DNA; D003513:Cycloheximide