Comparative study of various hydrogen ion buffers to assay Zn(2+)-dependent beta-lactamases.
The low Zn2+ complex formation constants, the capacity to degrade penicillin G in combination with Zn2+, and UV absorbance make 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 3-[N-tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid, 1,3-bis[tris(hydroxymethyl)methylamino]propane, and 1,4-piperazinediethanesulfonic acid convenient buffers to study Zn(2+)-dependent beta-lactamases.
Departamento de Química Fisica Aplicada, Universidad Autónoma de Madrid, Spain.
M J
MJ
Benitez
M
M
Company
A
A
Arevalillo
J S
JS
Jiménez
D001409:Bacillus cereus / Q000201:enzymology; D002021:Buffers; D007700:Kinetics; D010400:Penicillin G / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet; D015032:Zinc / Q000378:metabolism*; D001618:beta-Lactamases / Q000032:analysis*
D002021:Buffers; D001618:beta-Lactamases; D015032:Zinc; D010400:Penicillin G