Role of the galactose lectin of Entamoeba histolytica in adherence-dependent killing of mammalian cells.
Entamoeba histolytica extracellular killing of host cells is contact dependent. Adherence to human colonic epithelial cells and mucins is mediated by a galactose-specific lectin. The effect on cytotoxicity of a panel of monoclonal antibodies (MAb) directed against the galactose lectin was tested. As expected, those MAb which inhibited adherence also decreased cytotoxicity. However, one antilectin MAb blocked cytotoxicity after adherence had occurred, indicating that the lectin has a role in cell killing that is distinct from its adherence function.
Department of Medicine, University of Virginia Medical Center, Charlottesville 22908.
L D
LD
Saffer
W A
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Petri
D000268:Adhesiveness; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D016466:CHO Cells; D006224:Cricetinae; D004748:Entamoeba histolytica / Q000472:pathogenicity*; D005690:Galactose / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000502:physiology*; D015398:Signal Transduction
D000911:Antibodies, Monoclonal; D037102:Lectins; D005690:Galactose