Uteroferrin contains complex and high mannose-type oligosaccharides when synthesized in vitro.
Mature uteroferrin (Uf; Mr = 35,500) is a progesterone-induced acid phosphatase secreted by the pig uterus. It contains a single, unphosphorylated, high mannose-type oligosaccharide. Endometrial explants cultured in vitro secrete Uf with a Mr of 37,000 (37k Uf) having phosphorylated high mannose oligosaccharides. In this report we demonstrate that 37k Uf contains two N-linked oligosaccharides which are a mixture of complex and high mannose-type oligosaccharides. The complex-type glycopeptides are biantennary and a portion may be fucosylated on the GlcNac of the chitobiose core proximal to the peptide. Only a portion of the high mannose-type oligosaccharides are phosphorylated. The remainder appear to be typical Man6-4GlcNac2 oligosaccharides found on mature Uf.
Baumbach
G A
GA
Department of Animal Science, University of Tennessee, Knoxville 37901.
Saunders
P T
PT
Ketcham
C M
CM
Bazer
F W
FW
Roberts
R M
RM
D000135:Acid Phosphatase; D000595:Amino Acid Sequence; D000818:Animals; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000378:metabolism; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006031:Glycosylation; D006596:Hexosaminidases / Q000378:metabolism; D007527:Isoenzymes; D008358:Mannose / Q000737:chemistry*; D008667:Metalloproteins / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry*; D010766:Phosphorylation; D011233:Precipitin Tests; D013552:Swine; D000071681:Tartrate-Resistant Acid Phosphatase
D007527:Isoenzymes; D008667:Metalloproteins; D009844:Oligosaccharides; D003208:Concanavalin A; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D006596:Hexosaminidases; D008358:Mannose