Quantification of serum amyloid P by enzyme-linked immunosorbent assay.
This enzyme-linked immunosorbent assay procedure for quantifying serum amyloid P (SAP) in human plasma makes use of affinity-purified polyclonal antibodies to SAP in a "sandwich"-type format. The procedure is sensitive, reproducible, simple, and easily automatable. Results correlate well with those by a rocket immunoelectrophoresis method performed with the same antibodies. Sera from apparently normal individuals had a mean SAP content of 44.17 mg/L and increased with age.
Saïle
R
R
Laboratoire de Recherche Néphrologique, Hôpital Calmette, Lille, France.
Kandoussi
A
A
Deveaux
M
M
Descamps
J
J
Hachulla
E
E
Cachera
C
C
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D000683:Serum Amyloid P-Component / Q000032:analysis*; D012737:Sex Factors
D000683:Serum Amyloid P-Component