Differential response of the bvg virulence regulon of Bordetella pertussis to MgSO4 modulation.
Magnesium sulfate is known to repress the expression of the virulence factors of Bordetella pertussis that are coordinately regulated by the bvg locus. We have tested the time required by MgSO4 to repress the synthesis of several bvg-regulated mRNA species and found that the promoters of the virulence genes (pertussis toxin, adenylate cyclase, and filamentous hemagglutinin) are repressed in 6 min, while the autogenously regulated promoters of the bvg locus (P1, P3, and P4) are repressed only several hours later. These data show a differential behavior between regulated and autoregulated genes of the bvg regulon.
Sclavo Research Center, Siena, Italy.
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Rappuoli
D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000235:genetics; D001886:Bordetella pertussis / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005798:Genes, Bacterial / Q000187:drug effects; D005809:Genes, Regulator; D006388:Hemagglutinins / Q000235:genetics; D007700:Kinetics; D008278:Magnesium Sulfate / Q000494:pharmacology*; D037342:Pertussis Toxin; D011401:Promoter Regions, Genetic / Q000187:drug effects; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects; D014774:Virulence / Q000187:drug effects / Q000235:genetics*; D010566:Virulence Factors, Bordetella / Q000235:genetics
D037361:Adenylate Cyclase Toxin; D006388:Hemagglutinins; D012333:RNA, Messenger; D010566:Virulence Factors, Bordetella; D008278:Magnesium Sulfate; D037342:Pertussis Toxin; D000262:Adenylyl Cyclases