Effect of the position of the cyano-group of cyanopregnenolones on their drug metabolic inducing activity.
The effect of the position of the cyano-group of several cyanopregnenolones on the body's resistance to drugs and on drug metabolism was investigated. Female rats were pretreated with 2 alpha-, 6-, 16 alpha-, 17 alpha-cyano- or 16 alpha-cyanomethyl-pregnenolone or with pregnenolone, and the (in vivo) resistance to zoxazolamine, digitoxin and indomethacin, as well as the in vitro drug metabolism (post mitochondrial fraction) of zoxazolamine and ethylmorphine were determined. It was found that the 16-derivative was the most active in this respect, the 2- and 17-cyanopregnenolones were less active but significantly potent compared to controls, while the 6-cyano, the 16-cyanomethyl derivatives and pregnenolone were essentially inactive. These differences were explained in terms of an effective or poor fit of the steroids to their receptor. The poor performance of pregnenolone-16 alpha-acetonitrile was attributed to electronic effects. A hypothesis of some structural features of the receptor site for its interaction with the cyanopregnenolone inducers was presented.
Department of Pharmaceutical Chemistry, School of Pharmacy, University of Thessaloniki, Greece.
P N
PN
Kourounakis
E
E
Rekka
V J
VJ
Demopoulos
S
S
Retsas
D000097:Acetonitriles / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D004074:Digitoxin / Q000633:toxicity*; D004347:Drug Interactions; D004790:Enzyme Induction; D005036:Ethylmorphine / Q000378:metabolism*; D005260:Female; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D007213:Indomethacin / Q000633:toxicity*; D011284:Pregnenolone / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D011285:Pregnenolone Carbonitrile / Q000737:chemistry* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013329:Structure-Activity Relationship; D015049:Zoxazolamine / Q000378:metabolism / Q000633:toxicity*
D000097:Acetonitriles; C071461:pregnenolone 16-acetonitrile; D011285:Pregnenolone Carbonitrile; D011284:Pregnenolone; D015049:Zoxazolamine; D004074:Digitoxin; D005036:Ethylmorphine; D007213:Indomethacin