Identification of monoclonal antibody-binding domains within antigen P1 of Streptococcus mutans and cross-reactivity with related surface antigens of oral streptococci.
Eleven monoclonal antibodies (MAbs) specific for P1, the major protein surface antigen of Streptococcus mutans serotype c, were characterized by Western blot (immunoblot) analysis and by radioimmunoassay using whole bacterial cells. The approximate binding domains of the MAbs were determined by using full-length and truncated P1 polypeptides. The accessibility of these binding sites on the surfaces of intact bacteria was determined by radioimmunoassay. The ability of each MAb to cross-react with related proteins from strains of S. mutans serotypes e and f, S. sanguis, and S. sobrinus serotype g is also reported.
Brady
L J
LJ
Department of Oral Biology, University of Florida, Gainesville 32610.
Piacentini
D A
DA
Crowley
P J
PJ
Bleiweis
A S
AS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000276:immunology*; D001666:Binding Sites, Antibody; D003429:Cross Reactions; D008562:Membrane Glycoproteins; D051379:Mice; D009055:Mouth / Q000382:microbiology; D010446:Peptide Fragments / Q000276:immunology; D013291:Streptococcus / Q000276:immunology*; D013295:Streptococcus mutans / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; C055764:S-layer proteins