Porcine pancreatic cationic pro-elastase. Studies on the activation, turnover and interaction with plasma proteinase inhibitors.
Porcine pancreatic cationic pro-elastase was partly purified from pancreatic juice. The pro-enzyme binds slowly to alpha-macroglobulin and alpha 1-proteinase inhibitor. After 24 h incubation with plasma at room temperature more than 50% of the pro-elastase was still recovered in the form of free pro-enzyme. The pro-enzyme was activated by trypsin at neutral pH and by cathepsin B at pH 3.8. In the pig the half-life of i.v. administered pro-enzyme was about 30 min. After injection into the pancreatic duct radioactively labelled pro-elastase appeared in plasma within 30 min, and in peritoneal fluid after about 1 h.
Department of Surgical Pathophysiology, Malmö General Hospital, Sweden.
H O
HO
Håkansson
A
A
Borgström
K
K
Ohlsson
D000818:Animals; D001202:Ascitic Fluid / Q000201:enzymology; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D010179:Pancreas / Q000737:chemistry / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D011480:Protease Inhibitors / Q000097:blood; D013552:Swine; D014357:Trypsin / Q000494:pharmacology
D004792:Enzyme Precursors; D011480:Protease Inhibitors; C022443:proelastase; D010196:Pancreatic Elastase; D014357:Trypsin