Myocardial capillaries: increase in number by splitting of existing vessels.
To study myocardial vascular development, stereological parameters were estimated in 24 Wistar rat hearts of six different age groups, from newborn to adult. The vascular surface density showed a sharp increase in the first 2 weeks, a peak around the age of 2 weeks, and then a steady decrease until it flattened in adulthood. In contrast, the vascular volume percentage, when plotted against age, decreased continuously with the greatest change in the first week, after which the curve flattened. These findings are compatible with an increase in the number of capillaries with a concomitant decrease of their diameters. Qualitative scrutiny of the histology did indeed support the idea that vessels become thinner. Reconstructions of the histological sections showed the same change three dimensionally. The reconstructions also demonstrated very small holes that seemed to go through the capillaries in the younger stages. Corrosion casts of the blood vessels were made using a casting resin. This was injected into the umbilical artery of rat embryos from 15 days gestation to birth. In postnatal rats of six age groups methacrylate was injected directly into the left ventricle. These casts supported the stereological data by showing an increase in number and decrease in diameter of capillaries, while during pre- and postnatal development, the intervascular spaces lengthened from small, irregular spaces to long, rectangular ones. Small holes, the probable precursors of such spaces, were clearly visible in the wider vessels of the youngest stages. All data point to an interesting mode of capillary growth, i.e. growth by division of existing vessels.
Department of Anatomy and Embryology, University of Leiden, The Netherlands.
J P
JP
van Groningen
A C
AC
Wenink
L H
LH
Testers
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development; D003331:Coronary Vessels / Q000254:growth & development* / Q000648:ultrastructure; D006321:Heart / Q000254:growth & development*; D008833:Microcirculation / Q000254:growth & development; D008855:Microscopy, Electron, Scanning; D051381:Rats; D011919:Rats, Inbred Strains