[The inhibitory effect of cholecystokinin on phosphatidylcholine synthesis in isolated rat pancreatic acini].
The effects of cholecystokinin (CCK) and other pancreatic secretagogues on phosphatidylcholine (PC) synthesis were studied in isolated rat pancreatic acini. When acini were incubated with [3H] choline in the presence of 1 nM CCK-octapeptide (CCK8) for 60 min, the incorporations of [3H] choline to both water soluble choline metabolites and PC in acini were reduced by CCK8 to 74% and 41% of control, respectively. Pulse-chase study revealed that CCK reduced both the disappearance of phosphocholine and the synthesis of PC. Ca(2+)-mobilizing secretagogues such as carbamylcholine and Ca2+ ionophore A23187 also reduced PC synthesis to the same extent as CCK8. By contrast, neither cAMP-dependent secretagogues such as secretin and dibutyryl cAMP nor a phorbol ester had any effect on PC synthesis in acini. These results suggest that CCK inhibits PC synthesis by inducing both the reduction of choline uptake into acini and the inhibition of CTP: phosphocholine cytidylyltransferase activity. This hormonal regulation of PC synthesis via CDP-choline pathway appears to be mediated by Ca(2+)-dependent pathway but not by cAMP- or protein kinase C-dependent pathway.
Second Department of Internal Medicine, Kobe University School of Medicine.
T
T
Matozaki
C
C
Sakamoto
H
H
Nishisaki
Y
Y
Konda
O
O
Nakano
K
K
Matsuda
K
K
Wada
T
T
Suzuki
M
M
Nagao
M
M
Kasuga
D000818:Animals; D002766:Cholecystokinin / Q000494:pharmacology*; D003864:Depression, Chemical; D066298:In Vitro Techniques; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains
D010713:Phosphatidylcholines; D002766:Cholecystokinin