Cisplatin induces the small heat shock protein hsp25 and thermotolerance in Ehrlich ascites tumor cells.
Exposure of Ehrlich ascites tumor (EAT) cells to the anticancer drug cisplatin results in an elevated abundance of three isoforms of the small heat shock protein hsp25 without inducing the general stress response as commonly observed after heat shock. The most effective cisplatin concentration (2.5 microM) is also most efficient in arresting cells in S phase suggesting a relationship between hsp25 expression and cell cycle events. Exposure to cisplatin results also in an increased thermotolerance of EAT cells.
Institute of Molecular Biology, Department of Cell Physiology, Berlin-Buch, FRG.
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Oesterreich
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Schunck
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Benndorf
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Bielka
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology; D002452:Cell Count; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D014407:Tumor Cells, Cultured
D006360:Heat-Shock Proteins; D002945:Cisplatin