Gastric mucosal repair in vitro.
We tested whether cultured gastric mucosal cells would be suitable to study the two major steps of repair: restitution and proliferation. Preparations of freshly isolated epithelial cells of guinea pig gastric mucosa were used for the studies. The cells attached to Petri dishes within 10 h and formed monolayers after 48-72 h. Electron microscopy showed that each cell type was able to form lamellipodia (i.e., cell protrusions) during restitution in vivo. When monolayers were wounded with a razor blade, most cells at the edge of damage died within a few minutes, but some recovered from injury. Later, intact cells migrated from the edge into the denuded zone and restored the monolayer within 24-48 h. An increased number of cells near the edge started to synthesize DNA. In conclusion, this model allows one to study in vitro both aspects of mucosal repair.
Giebel
J
J
Department of Pharmacology, Medical School Hannover, Federal Republic of Germany.
Thiedemann
K U
KU
Schwenk
M
M
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004847:Epithelial Cells; D004848:Epithelium / Q000503:physiopathology; D005753:Gastric Mucosa / Q000503:physiopathology*; D006168:Guinea Pigs; D008297:Male; D013936:Thymidine / Q000378:metabolism; D014945:Wound Healing
D004247:DNA; D013936:Thymidine