Induction of plasmacytomas secreting antigen-specific monoclonal antibodies with a retrovirus expressing v-abl and c-myc.
ABL-MYC, a recombinant murine retrovirus that expresses v-abl and c-myc, rapidly induces transplantable mono- or oligoclonal plasmacytomas in BALB/c mice. To determine if the targets for transformation of this retrovirus are antigen-committed B lymphocytes and to explore this system as an alternative technique for producing antigen-specific monoclonal antibodies, plasmacytomas were induced in mice that had been immunized with two different types of immunogens, hen egg white lysozyme and sheep red blood cells. The majority of these plasmacytomas secreted immunogen-specific antibodies. Plasmacytomas induced in unimmunized mice did not react with hen egg white lysozyme or sheep red blood cells. The specific antibodies were comparable in concentration, specificity, and affinity to monoclonal antibodies obtained with conventional hybridoma technology, but, in addition to IgGs and IgMs, they included specific IgA antibodies, which are rare among splenic-derived hybridomas. Our results demonstrate that a principal target for ABL-MYC is an antigen-committed B lymphocyte. In addition this procedure provides an alternative method for the production of monoclonal antibodies, without a requirement for hetero-caryon formation by cell fusion techniques.
Molecular Genetics Section, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
E M
EM
Weissinger
H
H
Mischak
D A
DA
Largaespada
D A
DA
Kaehler
T
T
Mitchell
S J
SJ
Smith-Gill
R
R
Risser
J F
JF
Mushinski
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015152:Blotting, Northern; D015139:Blotting, Southern; D015870:Gene Expression; D051379:Mice; D008807:Mice, Inbred BALB C; D009113:Muramidase / Q000276:immunology; D016374:Oncogene Proteins v-abl / Q000235:genetics; D009857:Oncogenes; D010954:Plasmacytoma / Q000276:immunology*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics; D012190:Retroviridae / Q000235:genetics
D000911:Antibodies, Monoclonal; D016374:Oncogene Proteins v-abl; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D009113:Muramidase