A Pseudomonas aeruginosa alginate-exotoxin A conjugate that elicits anti-alginate and exotoxin A-neutralizing antibodies.
Pseudomonas aeruginosa alginate was covalently coupled to exotoxin A by reductive amination using adipic acid dihydrazide as spacer. The conjugate was composed of 25% alginate and 75% exotoxin A and possessed an average molecular mass higher than 700 kDa as determined by polyacrylamide gel electrophoresis. The conjugate had virtually no ADP-ribosyltransferase activity and a reduced cytotoxicity for TSA8 murine cells, derived from Friend erythroleukemia cells, as indicated by a greater than 50-fold increased LD50. Anti-conjugate antibodies recognized exotoxin A and alginate. A booster injection resulted in markedly increased antibody ELISA titers to both exotoxin A and alginate. The antibodies neutralized the exotoxin A toxicity.
Coin
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Laboratoire de Biochimie Microbienne, Université Claude Bernard, Lyon I, Villeurbanne, France.
Vacheron
M J
MJ
Guinand
M
M
Michel
G
G
D036002:ADP Ribose Transferases; D000464:Alginates / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D001427:Bacterial Toxins / Q000276:immunology*; D005098:Exotoxins / Q000276:immunology* / Q000302:isolation & purification / Q000494:pharmacology; D051379:Mice; D009500:Neutralization Tests; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D011817:Rabbits; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D000464:Alginates; D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D005098:Exotoxins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A