Rabbit antibodies against two different extracellular domains of human thyrotropin receptor possess thyroid stimulating activities.
We have produced rabbit antibodies against synthetic peptides corresponding to the mid-region (amino acid residues 172-202, C peptide) and to the unique segment near the transmembrane region (amino acid residues 341-370, P peptide) in the extracellular component of the human thyrotropin (TSH) receptor and evaluated their biological activities. Both anti-C peptide antibodies raised in two rabbits showed strong thyroid stimulating activities (TSAb) (4127% and 2548%). Anti-P peptide antibodies raised in two rabbits were also strongly positive for TSAb activities (359% and 3468%). However, none of these antibodies had TSH-binding inhibitor immunoglobulin (TBII) activities. These results suggest that the domains responsible for TSAb are likely to span the entire extracellular component of the TSH receptor.
Endo
T
T
Third Department of Internal Medicine, University of Yamanashi Medical School, Japan.
Ohmori
M
M
Ikeda
M
M
Kotani
S
S
Onaya
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D011989:Receptors, Thyrotropin / Q000276:immunology*; D013961:Thyroid Gland / Q000276:immunology*
D000906:Antibodies; D010455:Peptides; D011989:Receptors, Thyrotropin