Amino acid sequence of k Sci, the Bence Jones protein isolated from a patient with light chain deposition disease.
Light chain Sci was isolated from the urine of a patient affected by light chain deposition disease with an apparent exclusive localization to the kidney. Sci protein is an intact light chain: it consists of 214 amino acid residues and has an Mr of 23.65. Its complete primary structure has been determined by sequence analysis of the corresponding tryptic peptides and by partially sequencing the intact protein. Sequence comparison shows that Sci protein is strictly related to the light chains of kIIIa family (88% structural identity) which are usually expressed in autoimmune rheumatoid syndromes. Computer graphics model suggests a perturbation in k Sci three-dimensional structure due to the unusual replacement of residues 53 and 77.
Dipartimento di Biochimica, Università di Pavia, Italy.
V
V
Bellotti
M
M
Stoppini
G
G
Merlini
M C
MC
Zapponi
M L
ML
Meloni
G
G
Banfi
G
G
Ferri
D000368:Aged; D000595:Amino Acid Sequence; D001536:Bence Jones Protein / Q000737:chemistry* / Q000652:urine; D003196:Computer Graphics; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006801:Humans; D006942:Hypergammaglobulinemia / Q000276:immunology*; D007145:Immunoglobulin kappa-Chains / Q000097:blood / Q000737:chemistry* / Q000652:urine; D007674:Kidney Diseases / Q000276:immunology* / Q000473:pathology; D008297:Male; D008969:Molecular Sequence Data
D007145:Immunoglobulin kappa-Chains; D001536:Bence Jones Protein