The reactivity of EDTA, copper ion, and copper citrate with metallothioneins isolated from the digestive gland of cadmium-contaminated lobster (Homarus americanus).
The reactivity of EDTA, Cu2+, and copper citrate with two metallothionein preparations (MT-1 and MT-2) isolated from the digestive gland of Cd-contaminated American lobster (Homarus americanus) was studied. Under pseudo-first-order conditions, metallothioneins reacted with EDTA for removal of Cd2+ in a multiphasic manner. Cadmium(+II) removal by Cu2+ was complex and non-stoichiometric, suggesting different binding sites. Rabbit liver metallothionein reacted similarly. Cadmium removal from lobster metallothionein by copper citrate was slow and triphasic in nature. EDTA removed Cu2+ from lobster metallothionein very slowly.
Marine Chemistry Division, Scotia-Fundy Region, Department of Fisheries and Oceans, Halifax, N.S., Canada.
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D000818:Animals; D002104:Cadmium / Q000378:metabolism* / Q000494:pharmacology; D002951:Citrates / Q000494:pharmacology*; D019343:Citric Acid; D003300:Copper / Q000494:pharmacology*; D004064:Digestive System / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology*; D007700:Kinetics; D008668:Metallothionein / Q000302:isolation & purification / Q000378:metabolism*; D008121:Nephropidae; D011485:Protein Binding
D002951:Citrates; D002104:Cadmium; D019343:Citric Acid; D003300:Copper; D008668:Metallothionein; D004492:Edetic Acid