Determination of the 5' exon structure of the human cardiac alpha-myosin heavy chain gene.
We have deduced the exon structure of the 5' untranslated region of the human cardiac alpha-myosin heavy chain gene by cloning a cDNA for this region using the polymerase chain reaction. Comparison of the cDNA and genomic DNA sequences demonstrates that the 5' non-coding region of the alpha-myosin heavy chain gene is interrupted by two introns of 645 and 337 nucleotides. Secondly we have identified the transcriptional start-site by primer extension, corroborating the previous putative assignment for the alpha-myosin heavy chain promoter based on comparisons between the rat and human genes.
Department of Cardiothoracic Surgery, National Heart and Lung Institute, London, U.K.
N J
NJ
Brand
N
N
Dabhade
M
M
Yacoub
P J
PJ
Barton
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D005796:Genes; D006321:Heart / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D009218:Myosins / Q000235:genetics*; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D009838:Oligodeoxyribonucleotides; D004247:DNA; D009218:Myosins