Distribution of a negative regulator of haematopoietic stem cell proliferation (AcSDKP) and thymosin beta 4 in mouse tissues.
A competitive enzyme immunoassay using acetylcholinesterase as tracer for thymosin beta 4, has been developed. Using this assay and a previously described EIA for AcSDKP, a negative regulator of pluripotent haematopoietic stem cell proliferation, the levels of these two peptides were determined in mouse tissue extracts. The combination of EIAs with different HPLC procedures validated these methods and clearly demonstrated the ubiquity of these peptides in mouse tissues. Similar results are reported for rabbit thymus which suggest different hypotheses for AcSDKP biosynthesis.
Laboratoire d'Etudes Radioimmunologiques, SPI/DRIPP, CEN Saclay, Gif sur Yvette, France.
P
P
Pradelles
Y
Y
Frobert
C
C
Créminon
H
H
Ivonine
E
E
Frindel
D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division; D003429:Cross Reactions; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000032:analysis* / Q000302:isolation & purification; D009928:Organ Specificity; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013947:Thymosin / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D000099201:Thymosin beta(4)
D007106:Immune Sera; D009842:Oligopeptides; D013947:Thymosin; D000099201:Thymosin beta(4); C058504:goralatide