Analyses of bronchoalveolar lavage fluid (BALF) in MRL-lpr/lpr mice.
We performed bronchoalveolar lavage (BAL) in MRL-lpr/lpr (MRL/l) and MRL- +/+ (MRL/n) mice and evaluated various cellular and humoral components of the bronchoalveolar lavage fluid (BALF) to clarify the pathogenic mechanism of pulmonary fibrosis in MRL/l mouse. The numbers of macrophages, neutrophils and lymphocytes, N-Acetyl-beta-glucosaminidase (beta-NAG), and fibronectin increased in the BALF from MRL/l mice than that from MRL/n mice, but no significant differences were observed in total protein, beta-glucuronidase, acid phosphatase, or phospholipid level. Increased fibronectin level in the BALF from MRL/l mice may be related with pathogenesis of pulmonary fibrosis.
Department of Clinical Pathology, School of Medicine, Hiroshima University, Japan.
M
M
Takaishi
Y
Y
Awaya
S
S
Ishioka
S
S
Hozawa
T
T
Oyama
K
K
Takahashi
H
H
Maeda
M
M
Yamakido
D000118:Acetylglucosaminidase / Q000032:analysis; D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology*; D005353:Fibronectins / Q000032:analysis; D005966:Glucuronidase / Q000032:analysis; D007958:Leukocyte Count; D051379:Mice; D008815:Mice, Inbred Strains; D010743:Phospholipids / Q000032:analysis; D011506:Proteins / Q000032:analysis
D005353:Fibronectins; D010743:Phospholipids; D011506:Proteins; D000135:Acid Phosphatase; D005966:Glucuronidase; D000118:Acetylglucosaminidase