Monoclonal antibody to a specific antigen from Wolinella recta ATCC 33238.
Four monoclonal antibodies (m-Abs) to a specific antigen (150-kDa protein antigen) isolated from Wolinella recta ATCC 33238 by acid extraction were obtained. The four antibodies were all of the IgG1 subclass and exhibited equally high specificity for the antigen. A battery of 14 strains or oral bacteria were screened for cross-reactivity with each mAb by ELISA and Western blot analysis. Weak cross-reactivity to some strains was observed which differed depending upon the mAb. Immuno-electromicroscopic studies were performed with a mAb which revealed that the 150-kDa antigen was localized to the cell surface of W. recta ATCC 33238.
Department of Periodontology and Endodontology, Okayama University Dental School, Japan.
H
H
Kurihara
F
F
Nishimura
S
S
Kokeguchi
K
K
Kato
T E
TE
Van Dyke
Y
Y
Murayama
D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000302:isolation & purification; D000954:Antigens, Surface / Q000302:isolation & purification; D003429:Cross Reactions; D013045:Species Specificity; D016968:Wolinella / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface