Concentration of activated T lymphocytes in extracorporeal blood circulation for plasma separation.
To investigate whether the proportion of activated T lymphocytes changes in an extracorporeal blood flow, we compared paired samples collected at the inlet and outlet lines of an artificial circuit for plasma separation, using a dual-immunofluorescence flow cytometric technique. In our series of materials from patients with rheumatoid arthritis or systemic lupus erythematosus, the percentages of HLA-DR-positive cells in both CD4+ (P less than 0.01, n = 6) and CD8+ (P less than 0.05, n = 10) subsets were significantly increased in the outlet lines compared with the inlet lines, suggesting that activated T lymphocytes pass through an extracorporeal blood circuit more easily than resting T lymphocytes.
Third Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Japan.
S
S
Ijichi
M
M
Mishima
T
T
Matsuda
T
T
Izumihara
K
K
Kojima
T
T
Niimura
I
I
Maruyama
M
M
Osame
D000328:Adult; D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000628:therapy; D016516:CD4-CD8 Ratio; D003131:Combined Modality Therapy; D005112:Extracorporeal Circulation / Q000295:instrumentation; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007165:Immunosuppression Therapy; D007958:Leukocyte Count; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000628:therapy; D008213:Lymphocyte Activation; D008297:Male; D010956:Plasmapheresis / Q000295:instrumentation; D013601:T-Lymphocytes / Q000276:immunology*
D006684:HLA-DR Antigens