Yeast RNA polymerase II subunit RPB9 is essential for growth at temperature extremes.
The Saccharomyces cerevisiae RNA polymerase II subunit gene RPB9 was isolated and sequenced. RPB9 is a single copy gene on chromosome VII. The RPB9 sequence predicts a protein of 122 amino acids with a molecular mass of 14,200 Da. The yeast RPB9 subunit is similar in size and sequence to a protein encoded by DNA adjacent to the suppressor of the Hairy Wing gene in Drosophila melanogaster. Deletion of the RPB9 gene produced cells that were heat- and cold-sensitive. The RPB9 subunit, like the previously described RNA polymerase II subunit RPB4, is not essential for synthesis of mRNA, but is required for normal cell growth over a wide temperature range.
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
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Woychik
W S
WS
Lane
R A
RA
Young
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D004271:DNA, Fungal; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D012319:RNA Polymerase II / Q000235:genetics / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D016415:Sequence Alignment; D013696:Temperature
D004271:DNA, Fungal; D012319:RNA Polymerase II