[Enzyme immunoassay of urinary apolipoprotein H and its application for detecting incipient diabetic nephropathy].
In order to elucidate the clinical significance of urinary apolipoprotein H (Apo H), otherwise known as beta 2-glycoprotein I, we first developed a non-competitive enzyme immunoassay (EIA) to quantify urinary Apo H levels. The measurable range of this assay was about 2 ng/ml to 100 ng/ml. The intra- and inter-assay coefficients of variation were 2.0 to 4.7% and 8.3 to 17.0%, respectively. These results indicated that this assay had high sensitivity and good reproducibility, and that it was useful for clinical study. We then determined urinary Apo H levels in 24 normal subjects and 36 diabetics using this assay. The mean urinary Apo H index (urinary Apo H/cr ratio), in 20 patients without proteinuria, who were regarded as patients without nephropathy, was 569 +/- 560 (X10(-3) mg/g.cr) and significantly higher than in normal subjects (252 +/- 147, p less than 0.01). The mean urinary Apo H index in 16 patients with overt proteinuria was 1,507 +/- 3,701 and also higher than in normal subjects (p less than 0.01). These results taken collectively indicate that urinary Apo H level using the EIA may be a new sensitive marker for detecting minor change of glomerular basement membrane in diabetics.
Yoshimoto
Y
Y
2nd Department of Internal Medicine, Kochi Medical School, Nankoku.
Yoshida
K
K
Takamatsu
K
K
Yamano
T
T
Ohno
F
F
D000328:Adult; D000368:Aged; D003928:Diabetic Nephropathies / Q000175:diagnosis* / Q000652:urine; D005260:Female; D006023:Glycoproteins / Q000652:urine*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053482:beta 2-Glycoprotein I
D006023:Glycoproteins; D053482:beta 2-Glycoprotein I