Fixed human tissues: a resource for the identification of individuals.
Polymorphic genetic loci of the deoxyribonucleic acid (DNA) present in formalin-fixed, paraffin-embedded tissues were successfully analyzed by utilizing the polymerase chain reaction. Using this analysis, with three different polymorphic loci [human leucocyte antigen (HLA) DQ alpha, low-density lipoprotein receptor, and parathyroid hormone], fixed tissues representing 14 different individuals were genotyped and could be distinguished from each other. The techniques were further applied to the fixed autopsy tissues of a man in which a question of paternity arose postmortem. Since many individuals have surgical procedures or autopsy, these readily available fixed tissues represent an additional resource for the identification of individuals.
Shibata
D
D
Department of Pathology, University of Southern California School of Medicine, Los Angeles.
Kurosu
M
M
Noguchi
T T
TT
D000328:Adult; D000368:Aged; D000483:Alleles; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D016172:DNA Fingerprinting; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D005557:Formaldehyde; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000737:chemistry; D016612:Paraffin Embedding; D010281:Parathyroid Hormone / Q000235:genetics; D010334:Paternity; D016133:Polymerase Chain Reaction; D011973:Receptors, LDL / Q000235:genetics; D016707:Tissue Fixation
D006683:HLA-DQ Antigens; D010281:Parathyroid Hormone; D011973:Receptors, LDL; D005557:Formaldehyde; D004247:DNA