Heterogeneous IgE glycoforms characterized by differential recognition of an endogenous lectin (IgE-binding protein).
IgE is highly glycosylated, but the function of the oligosaccharide side chains is largely unknown. The previous discovery of an animal lectin, IgE-binding protein (epsilon BP), affords an opportunity to study potential carbohydrate-dependent effector functions of IgE. epsilon BP is a beta-galactoside-specific lectin with binding affinity for IgE and is now known to be equivalent to carbohydrate-binding protein 35 and the Mac-2 Ag; thus, it may have multiple functions in addition to IgE binding. We have previously shown that rat r epsilon BP recognizes sialidase-treated human myeloma IgE to a much greater extent than the untreated IgE. In contrast, human epsilon BP binds essentially equivalently to a monoclonal murine IgE with or without sialidase pretreatment. To validate a possible role for epsilon BP in the IgE system, we investigated the pattern of recognition of epsilon BP for various polyclonal human IgE samples. We show that polyclonal IgE derived from four individuals with hyper-IgE syndrome or atopic dermatitis recognizes epsilon BP and that there is individual variation in the proportion of IgE recognized by epsilon BP, ranging from greater than 60% for one sample to almost undetectable levels in another. We conclude that epsilon BP does indeed recognize polyclonal IgE and that this recognition is modulated by sialylation of IgE oligosaccharides. Furthermore, there exist different IgE glycoforms, varying in the degree of sialylation, and these are distributed in a distinct manner in different individuals.
Robertson
M W
MW
Department of Molecular and Experimental Medicine, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Liu
F T
FT
D000943:Antigens, Differentiation / Q000378:metabolism*; D037502:Galectin 3; D006801:Humans; D006942:Hypergammaglobulinemia / Q000378:metabolism; D006967:Hypersensitivity / Q000276:immunology; D007073:Immunoglobulin E / Q000737:chemistry* / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D011485:Protein Binding; D012795:Sialoglycoproteins / Q000737:chemistry / Q000378:metabolism
D000943:Antigens, Differentiation; D037502:Galectin 3; D037102:Lectins; D012795:Sialoglycoproteins; D007073:Immunoglobulin E