The role of transferrin and citrate in cellular uptake of aluminium.
The ability of human erythroleukaemia K562 cells to take up aluminium from Al-transferrin and Al-citrate has been examined. Uptake from Al-transferrin was dose-dependent over the range 68-544 ng/ml of aluminium, and increased over a 12-day period. In contrast, uptake from Al-citrate was low even at an aluminium concentration of 6800 ng/ml and did not increase over time. Neither form of aluminium greatly affected cell growth. It is concluded that Al-transferrin, rather than Al-citrate, is the physiologically relevant form of this metal with respect to cellular uptake, but that any metabolic abnormalities induced by aluminium do not affect proliferation of this cell line.
McGregor
S J
SJ
University Department of Immunology, Western Infirmary, Glasgow, Scotland, UK.
Brock
J H
JH
Halls
D
D
D000535:Aluminum / Q000378:metabolism*; D002455:Cell Division; D002951:Citrates / Q000378:metabolism / Q000502:physiology*; D019343:Citric Acid; D006801:Humans; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute; D015203:Reproducibility of Results; D014168:Transferrin / Q000378:metabolism / Q000502:physiology*; D014407:Tumor Cells, Cultured
D002951:Citrates; D014168:Transferrin; C066781:aluminum-transferrin; D019343:Citric Acid; D000535:Aluminum