Growth kinetics of Bacillus stearothermophilus BR219.
Bacillus stearothermophilus BR219, a phenol-resistant thermophile, can convert phenol to the specialty chemical catechol. The growth kinetics of this organism were studied in batch, continuous, and immobilized-cell culture. Batch growth was insensitive to pH between 6.0 and 8.0, but little growth occurred at 5.5. In continuous culture on a dilute medium supplemented with 10 mM phenol, several steady states were achieved between dilution rates of 0.25 and 1.3 h-1. Phenol degradation was found to be uncoupled from growth. Immobilized cells grew rapidly in a rich medium, but cell viability plummeted following a switch to a dilute medium supplemented with 5 mM phenol.
Department of Chemical Engineering, Michigan State University, East Lansing 48824-1226.
R M
RM
Worden
R
R
Subramanian
M J
MJ
Bly
S
S
Winter
C L
CL
Aronson
D001431:Bacteriological Techniques; D003470:Culture Media; D005285:Fermentation; D001411:Geobacillus stearothermophilus / Q000254:growth & development*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019800:Phenol; D010636:Phenols / Q000378:metabolism
D003470:Culture Media; D010636:Phenols; D019800:Phenol