Two proteins act as the IUF1 insulin gene enhancer binding factor.
IUF1 is a pancreatic beta cell-specific factor which binds to the sequence 5'-CPyCTAATG-3' (CT box) within the human insulin gene enhancer. Here we show that IUF1 is composed of 2 binding activities that can be separated by DEAE ion exchange chromatography. South Western blot analysis indicates that these distinct binding activities have apparent molecular weights of 115 kDa and 46 kDa.
Department of Medicine, University of Birmingham, Queen Elizabeth Hospital, UK.
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Scott
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Clark
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Hutton
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Docherty
D000818:Animals; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000502:physiology*; D004742:Enhancer Elements, Genetic; D006367:HeLa Cells; D006801:Humans; D007340:Insulinoma; D007515:Islets of Langerhans / Q000502:physiology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000737:chemistry / Q000502:physiology*; D009841:Oligonucleotides / Q000737:chemistry; D051381:Rats; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D009841:Oligonucleotides