Particle concentration fluorescence immunoassay of gentamicin.
We report the development of a particle concentration fluorescence immunoassay (PCFIA) for gentamicin using commercially available reagents. The solid phase consists of 0.8 microns polystyrene spheres to which gentamicin antibody is noncovalently bound. Fluorescein-labeled gentamicin serves as the tracer. The assay is carried out on 96-well reaction plates and read using an automated instrument. The assay is accurate over the range of gentamicin concentrations commonly encountered in clinical practice, with day-to-day coefficients of variation of 3-7%. As little as 0.01 mg/L of gentamicin can be detected when sample predilution is omitted. PCFIA is compared with Abbott TDX fluorescence polarization immunoassay for the measurement of serum gentamicin concentrations in routine clinical specimens.
St. Xavier College, Chicago, Illinois.
P
P
Varma-Nelson
J E
JE
Nelson
S M
SM
Belknap
D016231:Fluorescence Polarization Immunoassay / Q000379:methods; D005839:Gentamicins / Q000097:blood*; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards
D005839:Gentamicins; D011933:Reagent Kits, Diagnostic