Increased expression of class II major histocompatibility complex molecules on B cells in rats susceptible or resistant to HgCl2-induced autoimmunity.
Administration of HgCl2 to the susceptible Brown-Norway (BN) rats induces an autoimmune disease characterized by a T-dependent polyclonal activation of B cells responsible for a dramatic increase in serum IgE concentration. The resistant Lewis (LEW) rats injected with HgCl2 do not exhibit such autoimmune manifestations. We show here that, upon HgCl2 injections, major histocompatibility complex (MHC) class II molecule expression is increased very early in lymph nodes and spleen B cells from both strains. So far, it is the earliest marker (day 3) of the effect of HgCl2 on the immune system. In both strains this enhancement is transient, but regulatory mechanisms are much more efficient in the resistant LEW strain than in the susceptible BN strain. In addition, we observed that MHC class II molecule expression on B cells differs according to the organ and the rat strain tested. All these findings are discussed in an attempt to underline the role of MHC class II molecule expression in the occurrence of mercury-induced autoimmunity.
INSERM U28, Hôpital Broussais, Paris, France.
C
C
Dubey
B
B
Bellon
F
F
Hirsch
J
J
Kuhn
M C
MC
Vial
M
M
Goldman
P
P
Druet
D000818:Animals; D001327:Autoimmune Diseases / Q000139:chemically induced* / Q000276:immunology; D001402:B-Lymphocytes / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D007073:Immunoglobulin E / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology; D008627:Mercuric Chloride / Q000633:toxicity*; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D013154:Spleen / Q000276:immunology; D013997:Time Factors
D000949:Histocompatibility Antigens Class II; D007073:Immunoglobulin E; D008627:Mercuric Chloride