Posttranscriptional regulation of c-myc RNA during early development of Xenopus laevis.
The remarkable stability of c-myc during oogenesis contrasts with its degradation during the early developmental period in Xenopus laevis. Three evolutionary conserved motifs found in the 3'-untranslated region of Xenopus c-myc RNAs have been analyzed for a possible role in c-myc RNA degradation. No specific degradation was observed when these sequences were cloned downstream of a reporter gene and the corresponding RNAs were injected into fertilized eggs. The relation between polyadenylation and degradation of c-myc mRNA has been examined during early development. c-myc is adenylated during early oogenesis, and a dramatic de-adenylation occurs in full grown oocytes. Consequently, the de-adenylation of c-myc mRNA that occurs in eggs might be a requirement for its degradation after fertilization, but is not sufficient to trigger its degradation.
Institut Jacques Monod, Unité d'Embryologie Moléculaire, Paris, France.
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Tchang
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Vriz
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Méchali
D000818:Animals; D001483:Base Sequence; D004625:Embryo, Nonmammalian / Q000737:chemistry; D047108:Embryonic Development; D005260:Female; D016259:Genes, myc; D008969:Molecular Sequence Data; D009866:Oogenesis / Q000235:genetics; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D012323:RNA Processing, Post-Transcriptional; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics*
D011061:Poly A