Correlation between anthracycline structure and human DNA ligase inhibition.
A total of 19 anthracycline derivatives were tested for their ability to interfere in vitro with the action of the human replicative DNA ligase. Only those with the sugar devoid of unmodified amino groups or with large configurational modifications were found to be inactive. Maximal inhibition of DNA-joining activity was found to require a 4'-deoxy-3'-amino sugar. Self-adenylation of DNA ligase was largely insensitive to these drugs. An important general finding is that slight modifications of the anthracycline structure have pronounced effects on DNA-ligase-inhibitory activity and might be related to the specificity of anthracycline anti-tumour activity.
Istituto di Genetica Biochemica ed Evoluzionistica, C.N.R., Pavia, Italy.
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Ciarrocchi
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Lestingi
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Fontana
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Spadari
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Montecucco
D000249:Adenosine Monophosphate / Q000378:metabolism; D000903:Antibiotics, Antineoplastic / Q000737:chemistry*; D011088:DNA Ligases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D000903:Antibiotics, Antineoplastic; D000249:Adenosine Monophosphate; D011088:DNA Ligases