Efficiency of peptides and lipopeptides for in vivo priming of virus-specific cytotoxic T cells.
Synthetic peptides and a novel type of lipopeptide vaccine, both containing T cell epitopes recognized by Kd-restricted, influenza virus-specific cytotoxic T cells (CTL) were compared in their efficiency to induce virus-specific CTL in vivo. All attempts to induce virus-specific CTL with synthetic peptide (in the absence of adjuvants) failed. However, a latent immunization was observed, resulting in an increased response to the injected peptide seen only after boosting the recipients with immunogenic virus. In contrast, priming with synthetic lipopeptide vaccine (tripalmitoyl-S-glycerylcysteinyl-seryl-serine [P3CSS] coupled to peptide) was successful under most conditions, and matched the priming efficiency seen with infectious virus. The requirements for in vivo priming of virus-specific CTL using lipopeptide suggest that attachment of the lipopeptide to a hydrophobic entity, such as the cell membrane, is responsible for its efficiency.
Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, FRG.
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Schild
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Deres
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Wiesmüller
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Jung
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Rammensee
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D007111:Immunity, Cellular; D007156:Immunologic Memory; D009980:Influenza A virus / Q000276:immunology; D008074:Lipoproteins / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D010455:Peptides / Q000276:immunology*; D013329:Structure-Activity Relationship; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013997:Time Factors; D014758:Viral Core Proteins / Q000276:immunology
D000956:Antigens, Viral; D008074:Lipoproteins; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D010455:Peptides; D014758:Viral Core Proteins