Evaluation of a solid-phase cellular enzyme immunoassay for detection of the serologically defined male antigen.
An indirect cellular enzyme immunoassay for the detection of the serologically defined male-specific antigen, SDMA, was developed using mouse spermatozoa as the target. Serum from B6 female mice injected with male spleen cells was used as the source of SDMA-specific antibody. Our results indicate that the assay is highly reliable (96% accurate) with an intra- and interassay coefficient of variation less than 12%. Since this assay is non-subjective and simple to perform it provides a useful alternative to the complement-dependent cytotoxicity assay for detection of SDMA.
Faculty of Medicine, Memorial University of Newfoundland, St. John's, Canada.
B D
BD
Reilly
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EH
Goldberg
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D005260:Female; D006182:H-Y Antigen / Q000032:analysis*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013094:Spermatozoa / Q000276:immunology*; D013154:Spleen / Q000276:immunology
D006182:H-Y Antigen