Inhibitory effect of cadmium and mercury ions on transcription of the ada gene.
The effect of Cd2+ and Hg2+ on transcription of the ada gene was examined using an in vitro system reconstituted with Ada protein and methylated DNA, together with their effect on methyltransferase activity of the Ada protein. Both Cd2+ and Hg2+ inhibited transcription of the ada gene over the doses which did not inhibit methyltransferase activity.
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
M
M
Suzuki
K
K
Takahashi
Y
Y
Kawazoe
K
K
Sakumi
M
M
Sekiguchi
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002104:Cadmium / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000187:drug effects*; D007700:Kinetics; D008628:Mercury / Q000494:pharmacology*; D008780:Methyltransferases / Q000235:genetics* / Q000378:metabolism; D019853:O(6)-Methylguanine-DNA Methyltransferase; D014157:Transcription Factors; D014158:Transcription, Genetic / Q000187:drug effects*
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D014157:Transcription Factors; D002104:Cadmium; D008780:Methyltransferases; C045675:Ada protein, E coli; D019853:O(6)-Methylguanine-DNA Methyltransferase; D008628:Mercury