Isolation and characterization of temperature sensitive mutants of the F5 deletion mutant of mycobacteriophage D29.
Seven thermosensitive mutants of the F5 deletion mutant of the mycobacteriophage D29 were described. The mutants were obtained using N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) mutagenesis, and were characterized using temperature shift assays, complementation and recombination tests, electron microscopy of infected host bacteria at non-permissive temperature, and serum blocking power. Mutants deficient in tail assembly, and mutants deficient in head and tail assembly were described. Mutants deficient in head assembly but capable of assembling tails were not isolated during this study. From the data, 3 provisional linkage map of the phage F5 was proposed.
Unité de la Tuberculose des Mycobactéries, Institut Pasteur, Paris, France.
S
S
Sérès
R
R
Lazraq
H
H
Ohayon
D015153:Blotting, Western; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D005816:Genetic Complementation Test; D008040:Genetic Linkage; D008854:Microscopy, Electron; D016296:Mutagenesis; D009160:Mycobacteriophages / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D009170:Nontuberculous Mycobacteria; D011995:Recombination, Genetic; D013696:Temperature
D004279:DNA, Viral