Positive selection for uracil auxotrophs of the sulfur-dependent thermophilic archaebacterium Sulfolobus acidocaldarius by use of 5-fluoroorotic acid.
Uracil auxotrophs of Sulfolobus acidocaldarius were positively selected by using 5-fluoroorotic acid. The wild-type strain was unable to grow in medium containing 5-fluoroorotic acid, whereas the mutants grew normally. Positive selection could be done for the auxotrophs. Mutants deficient in orotidine-5'-monophosphate pyrophosphorylase activity were isolated.
Kondo
S
S
Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.
Yamagishi
A
A
Oshima
T
T
D003470:Culture Media; D005838:Genotype; D009154:Mutation; D009963:Orotic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D016945:Sulfolobus acidocaldarius / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D014498:Uracil / Q000378:metabolism*
D003470:Culture Media; D014498:Uracil; D009963:Orotic Acid; C001242:5-fluoroorotic acid; D009964:Orotidine-5'-Phosphate Decarboxylase