Onset and regulation of anti-lamin B autoantibody production is independent of the level of polyclonal activation.
Anti-lamin B autoantibodies are associated with both systemic lupus erythematosus (SLE) and autoimmune liver disease. We examined the possibility that the underlying clinical feature in patients with anti-lamin B autoantibodies might be chronic autoimmune liver disease, and whether the hypergammaglobulinemia present in both disorders is involved in generating anti-lamin B autoantibodies. A lamin B fusion protein (MLB1), consisting of amino acids 77-533 of lamin B fused to TrpE, was used to screen sera from 84 patients with SLE for anti-lamin B autoantibodies. 3/4 prototype human lamin B antisera, 5/84 SLE sera (6%), and 0/30 sera from healthy individuals reacted with MLB1 on immunoblots at a 1:500 dilution. Of the 9 anti-lamin B autoantibody positive patients studied, all but 1 fulfilled at least four ARA criteria for SLE. None of the patients displayed evidence of chronic autoimmune liver disease, suggesting that autoimmune liver disease is not strongly associated with anti-lamin B antibodies in SLE. In SLE, as in "lupoid hepatitis", anti-lamin B autoantibodies are often produced transiently during periods of increased disease activity. Although polyclonal hypergammaglobulinemia is also associated with increased activity of both diseases, anti-lamin B autoantibody production in 2 patients was independent of total immunoglobulin levels, antibodies to irrelevant proteins, and production of some other autoantibodies. Thus, polyclonal activation is insufficient to explain either the initiation or regulation of anti-lamin B autoantibody production, supporting the hypothesis that antinuclear antibodies are antigen-selective.
Laboratory of Cell Biology, Rockefeller University, New York, NY 10021.
C H
CH
Chou
S A
SA
Ali
R
R
Roubey
J
J
Buyon
W H
WH
Reeves
D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000096:biosynthesis*; D001483:Base Sequence; D015153:Blotting, Western; D003165:Complement System Proteins / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D006942:Hypergammaglobulinemia / Q000276:immunology; D034921:Lamin Type B; D034882:Lamins; D008107:Liver Diseases / Q000276:immunology; D008137:Longitudinal Studies; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000276:immunology*; D015345:Oligonucleotide Probes; D011993:Recombinant Fusion Proteins / Q000276:immunology
D001323:Autoantibodies; D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins; D015345:Oligonucleotide Probes; D011993:Recombinant Fusion Proteins; D003165:Complement System Proteins