Survival of Prevotella intermedia (Bacteroides intermedius) in transport media.
Five transport media were selected for testing, in vitro, the survival of a pure strain of Prevotella intermedia (Bacteroides intermedius) for 6, 24, and 72 hours. Two were non-nutrient transport media (RTF and VMG IV). The three others (TG, PY, PYG) were nutrient media. An increase in the transport time, and manipulations in an aerobic atmosphere compromised the survival of the bacteria. PY ensured good survival of the bacteria. RTF seems to be the best medium for the transport of P. intermedia, when quantitative analysis is required.
Département d'Odontologie Conservatrice-Endodontie, U.F.R. d'Odontologie, Université de Lille II, France.
E
E
Deveaux
P
P
Delplanque
C
C
Neut
C
C
Romond
D000328:Adult; D000332:Aerobiosis; D001431:Bacteriological Techniques; D001439:Bacteroides / Q000502:physiology*; D015169:Colony Count, Microbial; D003470:Culture Media / Q000032:analysis; D006801:Humans; D010514:Periodontal Pocket / Q000382:microbiology; D010518:Periodontitis / Q000382:microbiology; D013048:Specimen Handling / Q000379:methods; D013997:Time Factors
D003470:Culture Media