Deficient antiendometrium lymphocyte-mediated cytotoxicity in patients with endometriosis.
OBJECTIVE
To study the possible role of the immune system in the pathogenesis of endometriosis.


METHODS
A cytotoxicity assay by 51Cr release was performed to determine the lymphocyte cytotoxic response toward endometrial targets and an erytroleukemic cell line (K562).


METHODS
The assays were performed in an academic research environment.


METHODS
Twenty-five control women and 25 patients with endometriosis were selected on the basis of laparoscopic examination.


METHODS
The lymphocyte cytotoxic activity was evaluated separately on endometrial stromal and epithelial cells after 4 hours' incubation.


METHODS
The study was designed to determine, in controls and endometriosis patients, the lymphocyte-mediated cytotoxicity toward stromal and epithelial cells of endometrium.


RESULTS
The lymphocyte response in the presence of stromal cell antigens was significantly lower (P less than 0.02) in disease-affected women when compared with that obtained in controls (2.89 +/- 0.87 and 7.64 +/- 1.66, respectively). In contrast, when the same assay was performed on K562 cells, no difference was observed between endometriosis patients and controls.


CONCLUSIONS
These data suggest that an altered immune recognition might be one of the pathogenic mechanisms of endometriosis. Moreover, they indicate that this is not a general phenomenon but is specific for the endometrial target.
III Department of Obstetrics and Gynecology, University of Milano, Italy.
P
P
Viganò
P
P
Vercellini
A M
AM
Di Blasio
A
A
Colombo
G B
GB
Candiani
M
M
Vignali
D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D004715:Endometriosis / Q000276:immunology* / Q000473:pathology; D004717:Endometrium / Q000276:immunology* / Q000473:pathology; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural / Q000502:physiology; D016131:Lymphocyte Subsets / Q000276:immunology; D008214:Lymphocytes / Q000502:physiology*
D015703:Antigens, CD