Bioavailability of folate following ingestion of cholestyramine in the rat.
The effect of cholestyramine ingestion on the intestinal deconjugation and absorption of folic acid (PGA) and brewers yeast folate was investigated using a rat bioassay and liver folate uptake as the response parameter. Male weanling Sprague Dawley rats were depleted on a low AIN-76A formulated basal diet for 21 days. During a 14 day repletion period folic acid (PGA) and brewers yeast were added to provide 0.25, 0.5 and 1.0 mg of folate per kg of diet. Cholestyramine was administered directly as part of the diet at 1.1%. All diets were made isonitrogenous and isocaloric. Based on a parallel line assay, the relative biological value of folate for PGA + cholestyramine (79) was significantly different from the standard diet (PGA = 100), while those for brewers yeast (88) and for brewers yeast + cholestyramine (88) did not differ from the standard diet. Ingestion of cholestyramine significantly reduced the bioavailability of PGA versus brewers yeast folate in rats.
Nutrition Research Division, Health and Welfare, Ottawa, Ontario, Canada.
K
K
Hoppner
B
B
Lampi
D000818:Animals; D001682:Biological Availability; D002792:Cholestyramine Resin / Q000008:administration & dosage*; D004435:Eating; D005492:Folic Acid / Q000493:pharmacokinetics*; D007408:Intestinal Absorption / Q000187:drug effects; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D002792:Cholestyramine Resin; D005492:Folic Acid