Determination of the DNA-interacting region of the archaebacterial chromosomal protein MC1. Photocrosslinks with 5-bromouracil-substituted DNA.
Protein MC1 is the major chromosomal protein in methanosarcinaceae. Using photochemical crosslinking on 5-bromouracil-substituted DNA, we identified the region of the protein that interacts with it. This region is located in the C-terminal part of the polypeptide chain, and the crosslinked amino-acids are in the region 74-86. Tryptophan 74 is one of the amino-acids crosslinked to DNA.
Katouzian-Safadi
M
M
Centre de Biophysique Moléculaire, CNRS, Orléans, France.
Laine
B
B
Chartier
F
F
Cremet
J Y
JY
Belaiche
D
D
Sautiere
P
P
Charlier
M
M
D019843:Archaeal Proteins; D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D001976:Bromouracil; D003432:Cross-Linking Reagents; D004247:DNA / Q000378:metabolism*; D017020:Methanosarcina / Q000737:chemistry*; D010777:Photochemistry; D012261:Ribonucleoproteins / Q000378:metabolism*
D019843:Archaeal Proteins; D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; C051211:HMb protein, Methanosarcina; D012261:Ribonucleoproteins; D001976:Bromouracil; D004247:DNA