Insulin-like growth factor I-like immunoreactivity in serum and tissues of the tilapia, Oreochromis mossambicus.
Tilapia serum was acidified with 0.5 M HCl and then chromatographed on an octadecasily-silica column. After washing with 4% acetic acid, the column was eluted with methanol. The eluate was evaporated to dryness. The sample cross-reacted in a human insulin-like growth factor I (IGF-I) radioimmunoassay, suggesting immunochemical similarity to human IGF-I. IGF-I-like immunoreactivity was present at high levels in tilapia liver. Other tissues containing IGF-I-like immunoreactivity included the gonad, kidney, heart, spleen, brain and muscle. The serum IGF-I-like immunoreactivity was attributed to substances with a molecular weight of 9,000 and 45,000 respectively, and it was elevated after treatment with bovine growth hormone and carp pituitary extract.
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Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T.
Leung
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Woo
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D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D013006:Growth Hormone / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D008099:Liver / Q000737:chemistry; D008970:Molecular Weight; D010473:Perciformes / Q000097:blood / Q000378:metabolism*; D010902:Pituitary Gland / Q000737:chemistry; D011863:Radioimmunoassay; D014020:Tissue Extracts / Q000494:pharmacology
D014020:Tissue Extracts; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone