Colocalization of N-CAM and N-cadherin in avian skeletal myoblasts.
The cell-cell adhesion molecules, N-CAM and N-cadherin, have been shown previously to mediate myoblast interaction during cell fusion accompanying skeletal myogenesis. To study the localization of both molecules in fusion-competent myoblasts, we used antigen-specific primary antibodies and a double-labeling preembedding immuno-electron microscopy technique. Ultrastructural observations and quantitative analysis of the results reveal that N-CAM and N-cadherin frequently colocalize in clusters on the myoblast plasma membrane. The data provide morphological evidence that the two adhesion glycoproteins cooperate in mediating myoblast interaction during myoblast fusion.
Lankenau Medical Research Center, Wynnewood, Pennsylvania 19096.
A P
AP
Soler
K A
KA
Knudsen
D000818:Animals; D015820:Cadherins / Q000032:analysis*; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis*; D002478:Cells, Cultured; D002642:Chick Embryo; D016253:Microscopy, Immunoelectron; D009132:Muscles / Q000737:chemistry* / Q000196:embryology / Q000648:ultrastructure
D015820:Cadherins; D015816:Cell Adhesion Molecules, Neuronal