The identification and purification of the heterotrimeric GTP-binding protein from squid (Loligo forbesi) photoreceptors.
The principal GTP-binding protein (G-protein) from squid (Loligo forbesi) photoreceptor membranes has been identified by amino acid sequencing. The heterotrimeric protein was purified by detergent solubilization and ion-exchange chromatography. The amino acid sequence of the G-protein alpha-subunit (G-alpha) indicates that this subunit is closely related to the recently characterized Gq subgroup, whereas the G-gamma subunit varies widely in sequence from other homologues.
Department of Biochemistry and Molecular Biology, University of Leeds, U.K.
J D
JD
Pottinger
N J
NJ
Ryba
J N
JN
Keen
J B
JB
Findlay
D000595:Amino Acid Sequence; D000818:Animals; D002852:Chromatography, Ion Exchange; D003488:Cyanogen Bromide; D049832:Decapodiformes; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification*; D007463:Iodobenzoates / Q000737:chemistry; D008969:Molecular Sequence Data; D010786:Photoreceptor Cells / Q000737:chemistry*; D012689:Sequence Homology, Nucleic Acid; D014508:Urea / Q000737:chemistry
D003902:Detergents; D007463:Iodobenzoates; D014508:Urea; D019204:GTP-Binding Proteins; D003488:Cyanogen Bromide