Development of a solid phase enzyme immunoassay for the detection of anti-Ro autoantibodies.
An enzyme immunoassay was developed to detect anti-Ro(SS-A) autoantibodies. Both Ro-antigen components (52 and 60 kD) were purified from a pig spleen extract, using fast protein liquid chromatography (FPLC). Anti-La, anti-RNP, anti-DNA and anti-Sm antibodies do not react to the purified antigen. There was a strong correlation between anti-Ro activity in EIA and the titers in counter immunoelectrophoresis (rs = 0.893). Anti-Ro antibodies were found in 54 (69.2%) of 78 SLE sera by the developed EIA.
Dörner
T
T
Department of Internal Medicine, Medical School (Charité), Humboldt University Berlin, Germany.
Hiepe
F
F
Mielke
F
F
Kiessig
S T
ST
Lukowsky
A
A
Apostoloff
E
E
D000818:Animals; D001323:Autoantibodies / Q000032:analysis* / Q000302:isolation & purification; D001324:Autoantigens / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D002853:Chromatography, Liquid / Q000379:methods; D003378:Counterimmunoelectrophoresis; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008970:Molecular Weight; D020733:RNA, Small Cytoplasmic; D012016:Reference Values; D012261:Ribonucleoproteins; D013552:Swine; D000099207:SS-A Antigen
D001323:Autoantibodies; D001324:Autoantigens; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human