A combined study of aggregation, membrane affinity and pore activity of natural and modified melittin.
The pore activity of melittin and several chemically modified derivatives has been investigated using conductance measurements on planar lipid bilayers and marker release from small unilamellar vesicles. The modifications included N-terminal formylation, acetylation, succinylation and modification of the tryptophan residue. All of the compounds showed bilayer permeabilizing properties, though quantitative differences were evident. These comprised changes in the voltage dependence of the conductance, in the single-pore kinetics, in the concentration of aqueous peptide required to induce a given pore activity and in the apparent 'molecularity' reflected by the power law of its concentration dependence. A strong tendency for disrupting bilayers was not always correlated with strong pore activity. For a better understanding of these results, measurements of pore activity were complemented by studying the aggregation behavior in solution and the water-membrane partition equilibrium. Modifications of charged residues gave rise to significant changes in the aggregation properties, had virtually no influence on the partition coefficient. The latter decreased strongly, however, as a result of tryptophan modification. Analysis of the isotherms was consistent with the assumption that the arginine residues in melittin do not contribute very much to charge accumulation at the immediate membrane/water interface.
Department of Biophysical Chemistry, Biocenter of the University, Basel, Switzerland.
S
S
Stankowski
M
M
Pawlak
E
E
Kaisheva
C H
CH
Robert
G
G
Schwarz
D000107:Acetylation; D002942:Circular Dichroism; D008051:Lipid Bilayers / Q000737:chemistry*; D008555:Melitten / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry*; D010539:Permeability; D013329:Structure-Activity Relationship; D013386:Succinates; D014364:Tryptophan / Q000737:chemistry; D014461:Ultracentrifugation
D008051:Lipid Bilayers; D008563:Membrane Lipids; D013386:Succinates; D008555:Melitten; D014364:Tryptophan