[The characteristic of the distribution of C apolipoproteins in umbilical cord blood].
The profiles of C-II apolipoprotein (apo C-II) or apo C-III were determined in ten normal full-term female neonates and ten healthy adult females, to characterize the distribution of apo C in umbilical cord blood (UCB). The profiles of the distribution of apo C were electrophoretically obtained. After agarose gel-immunofixation electrophoresis using anti-apo C-II or anti-apo C-III antisera, the immune complexes of apo C-II or apo C-III selectively remained in the gels by salting out the non-immunoreacted proteins and were stained to be measured by the densitometry. So, apo C in high density lipoprotein (HDL) were satisfactorily separated from apo C in apo B-containing lipoproteins (LPcB). The apolipoproteins levels in mg/dl +/- SD for all UCB were; apo A-I = 84.4 +/- 11.8; apo A-II = 17.8 +/- 5.9; apo B = 14.0 +/- 4.2; apo C-II = 2.4 +/- 0.4; apo C-III = 3.6 +/- 0.9; apo E = 3.7 +/- 1.2, and these values were, respectively, 62.5%, 55.3%, 15.9%, 68.6%, 48.0% and 90.2% of the values obtained in adult blood. Apo C profiles in UCB were characterized by the distributions of the majority of apo C to HDL, in the same manner as the distribution of apo E, and were similar to the apo C profiles acquired from adult patients with hypo-beta lipoproteinemias. The percentages of apo C-II and apo C-III in umbilical HDL to total apo C-II and total apo C-III were high, 90.3% and 91.8%, compared to the adult HDL levels of 52.4% and 51.5%, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
Zenkokai Ikeda Hospital, Sakai.
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Ikeda
D000328:Adult; D053304:Apolipoprotein C-II; D053305:Apolipoprotein C-III; D001056:Apolipoproteins C / Q000097:blood*; D004587:Electrophoresis, Agar Gel; D005260:Female; D005312:Fetal Blood / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D011247:Pregnancy
D053304:Apolipoprotein C-II; D053305:Apolipoprotein C-III; D001056:Apolipoproteins C