Idiotypic and immunochemical differences of anti-DNA antibodies of a lupus patient during active and inactive disease.
IgG anti-DNA antibodies of a lupus patient during active and inactive stages of her disease were studied. There were no significant differences in the amounts, in double-stranded DNA-binding activity, or in complement-fixing ability between purified IgG anti-DNA antibodies of both stages. However, their idiotype (Id) expressions were different as revealed by binding to rabbit anti-Ids raised against each of the anti-DNA antibodies. Moreover, the active stage anti-DNA antibodies had higher activity and less cross-reactivity with polynucleotides. Dominant anti-DNA populations during the active and inactive stages of systemic lupus erythematosus are clonally and qualitatively different.
Innunology Research Laboratory, St. Luke's Hospital, Kansas City, Missouri 64111.
R
R
Suenaga
M
M
Evans
N I
NI
Abdou
D000974:Antibodies, Antinuclear / Q000032:analysis*; D000918:Antibody Specificity; D003168:Complement Fixation Tests; D003429:Cross Reactions; D004247:DNA / Q000276:immunology*; D005260:Female; D006801:Humans; D007120:Immunochemistry; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000097:blood*
D000974:Antibodies, Antinuclear; D007130:Immunoglobulin Idiotypes; D004247:DNA