Localization of calmodulin in perinuclear structures of spermatids and spermatozoa: a comparison of six mammalian species.
The distribution of Calmodulin was examined during spermiogenesis and sperm epididymal maturation in rabbit, hamster, mouse, rat, monkey, and human. An affinity-purified antibody to Calmodulin was used to characterize this protein in sperm extracts by immunoblot analysis. Post-embedding immunogold procedures were used to localize Calmodulin at the ultrastructural level. The pattern of Calmodulin distribution was similar in the six species studied. A diffuse labeling was observed in round spermatids. Gold particles accumulated first in the subacrosomal layer of elongating spermatids. The perinuclear ring was also labeled. During the maturation phase of spermatids, Calmodulin labeling extended to the postacrosomal sheath. Dramatic changes occurred at spermiation so that in testicular sperm Calmodulin immunostaining was predominant in the postacrosomal sheath. Some labeling was still detected in restricted areas of the subacrosomal layer. This feature varied from species to species. Calmodulin location did not change during sperm epididymal maturation. A role for Calmodulin in the control of manchette development and regulation of subacrosomal actin aggregation state during spermiogenesis is proposed. The unique location of Calmodulin in the postacrosomal sheath of all species that have been studied in this work, together with the known presence of calcium in this area suggest a pivotal role for Calmodulin in sperm-egg fusion process.
Groupe d'Etude de la Formation et de la Maturation du Gamète Mâle, UFR Biomédicale des Saints-Pères, Paris, France.
M L
ML
Kann
J
J
Feinberg
D
D
Rainteau
J P
JP
Dadoune
S
S
Weinman
J P
JP
Fouquet
D000177:Acrosome / Q000737:chemistry / Q000276:immunology / Q000648:ultrastructure; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002147:Calmodulin / Q000032:analysis* / Q000276:immunology; D002467:Cell Nucleus / Q000737:chemistry* / Q000276:immunology / Q000648:ultrastructure; D006224:Cricetinae; D006046:Gold; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry / Q000379:methods; D008252:Macaca fascicularis; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D011817:Rabbits; D051381:Rats; D013087:Spermatids / Q000737:chemistry* / Q000276:immunology / Q000648:ultrastructure; D013094:Spermatozoa / Q000737:chemistry* / Q000276:immunology / Q000648:ultrastructure
D000906:Antibodies; D002147:Calmodulin; D006046:Gold