Measurement of the concentration of amphotericin B in brain tissue of scrapie-infected hamsters with a simple and sensitive method.
A simple, sensitive, and reproducible assay for the measurement of the amphotericin B concentration in tissue extracts was developed by using the fourth derivative of the absorption spectrum of amphotericin B between wavelengths of 330 and 430 nm. The amphotericin B concentration in spleen and brain was proportional to the total amount administered. The amphotericin B concentration in the brain was highly correlated with the increase in the mean incubation period of intracerebrally scrapie-infected hamsters.
Institute of General Pathology, Catholic University, Rome, Italy.
P
P
Casaccia
A
A
Ladogana
Y G
YG
Xi
L
L
Ingrosso
M
M
Pocchiari
M C
MC
Silvestrini
A
A
Cittadini
D000666:Amphotericin B / Q000032:analysis / Q000493:pharmacokinetics*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D006224:Cricetinae; D008647:Mesocricetus; D012608:Scrapie / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013154:Spleen / Q000737:chemistry
D000666:Amphotericin B