Anti-FD4 idiotypic antibody mimicking human gastric cancer-associated antigen.
Rabbits were immunized with the Fab fragment of a murine monoclonal antibody (McAb) PD4 against human gastric cancer to produce anti-PD4-idiotypic antibody (alpha PD4-Ab2). The alpha PD4-Ab2 could not only competitively inhibit binding of McAb PD4 to gastric cancer cell MGC803, but also induce delayed-type hypersensitivity (DTH) to MGC803 in mice. Spleen cells of mice immunized with alpha PD4-Ab2 were fused with myeloma cell SP2/0 to form hybridoma secreting Ab3 which could be bound to target cell MGC803. McAb C7-Ab3, one of the Ab3, could selectively react with a 40 kD tumor-associated antigen located on MGC803 cell membrane, as well as McAb PD4. The results indicate that alpha PD4-Ab2 possesses determinants (internal image antigen) similar to those on MGC803, and could mimic human gastric cancer-associated antigen.
Department of Biochemistry and Molecular Biology, Institute for Cancer Research, Beijing.
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Shou
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Dong
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Liu
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000912:Antibodies, Neoplasm / Q000276:immunology*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology*; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D006968:Hypersensitivity, Delayed / Q000276:immunology; D016056:Immunodominant Epitopes / Q000276:immunology; D007128:Immunoglobulin Fragments / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D013274:Stomach Neoplasms / Q000276:immunology* / Q000473:pathology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D016056:Immunodominant Epitopes; D007128:Immunoglobulin Fragments; C100277:immunoglobulins, Fd