Interleukin 1 expression is inducible by nerve growth factor in PC12 pheochromocytoma cells.
Expression of the cytokine interleukin 1 alpha (IL-1 alpha) was demonstrated in the rat PC12 pheochromocytoma cell line by (i) immunohistochemistry using rabbit polyclonal antisera raised against the recombinant murine IL-1 alpha, (ii) an ELISA, and (iii) a specific cell conversion bioassay based on the use of LBRM33-1A5 cells. IL-1 alpha mRNA was demonstrated in the PC12 cells, by PCR amplification. Constitutive expression of IL-1 alpha in PC12 cells was demonstrated in all experiments, although the cellular levels of IL-1 alpha-like immunoreactivity varied. The expression of IL-1 alpha, as studied at the mRNA level, was inducible by mouse nerve growth factor (7S NGF), and the gene product level was inducible in a dose- and time-dependent fashion by 7S NGF. The maximum induction corresponds to a 600% increase in IL-1 alpha-like immunoreactivity above the expression level found in noninduced cells and occurred after a 3-day incubation of the cells with NGF at 0.75 micrograms/ml of culture medium. The significance of the ability of NGF to induce IL-1 expression lies in the fact that IL-1 itself also acts as a growth factor that promotes glial proliferation and, even more importantly, IL-1 itself induces the expression of NGF at peripheral nerve injury [Lindholm, D., Heumann, R., Meyer, M. & Thoenen, H. (1987) Nature (London) 330, 658-659].
Department of Biochemistry, Arrheniuslaboratory, Stockholm University, Sweden.
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K
Alheim
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C
Andersson
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Tingsborg
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Ziolkowska
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Schultzberg
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Bartfai
D000818:Animals; D000906:Antibodies; D001483:Base Sequence; D001681:Biological Assay; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000276:immunology / Q000494:pharmacology*; D009838:Oligodeoxyribonucleotides; D016716:PC12 Cells; D016133:Polymerase Chain Reaction / Q000379:methods; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000032:analysis
D000906:Antibodies; D007375:Interleukin-1; D009414:Nerve Growth Factors; D009838:Oligodeoxyribonucleotides; D012334:RNA, Neoplasm; D011994:Recombinant Proteins