Serologic enzyme-linked immunosorbent assay responses of calves vaccinated with a killed Mycobacterium paratuberculosis vaccine.
The purpose of this study was to document the effect of calfhood vaccination for Mycobacterium paratuberculosis on a serologic ELISA. Fifteen calves vaccinated with a killed paratuberculosis vaccine and 5 unvaccinated control calves were tested from the first through the fifteenth month of life. Age of vaccination ranged from 5 to 40 days. Blood samples were collected prior to vaccination and periodically thereafter. Serum antibody was analyzed by use of the ELISA. All calves were ELISA-negative prior to vaccination. Thirteen of 15 vaccinated calves became ELISA-positive between 2 and 6 months after vaccination. The unvaccinated cohort remained ELISA-negative. Wide-spread use of vaccine may interfere with diagnosis of paratuberculosis and with control programs that are based on serologic tests that measure humoral antibody.
Spangler
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Department of Veterinary Preventive Medicine, College of Veterinary Medicine, Ohio State University, Columbus 43210.
Heider
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Bech-Nielsen
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Dorn
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CR
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001428:Bacterial Vaccines / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology* / Q000302:isolation & purification; D010283:Paratuberculosis / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines