Functional mapping of the human immunodeficiency virus type 1 Rev RNA binding domain: new insights into the domain structure of Rev and Rex.
Expression of human immunodeficiency virus type 1 (HIV-1) structural proteins requires the direct interaction of the viral trans-activator protein Rev with its cis-acting RNA sequence (Rev-response element [RRE]). A stretch of 14 amino acid residues of the 116-amino-acid Rev protein is sufficient to impose nucleolar localization onto a heterologous protein. Our results demonstrated that these same amino acid residues confer Rev-specific RRE binding to the heterologous human T-cell leukemia virus type I Rex protein. In addition, our results indicated that amino acids distinct from the nuclear localization signal are important for Rex-specific RRE RNA binding.
Böhnlein
E
E
SANDOZ Research Institute, Vienna, Austria.
Berger
J
J
Hauber
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D015694:Gene Products, rev / Q000235:genetics* / Q000378:metabolism; D016370:Gene Products, rex / Q000235:genetics* / Q000378:metabolism; D016355:Genes, pX; D015757:Genes, rev; D015497:HIV-1 / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D016370:Gene Products, rex; D012367:RNA, Viral; D054321:rev Gene Products, Human Immunodeficiency Virus