Immunological characterization of canine hematopoietic progenitor cells.
Canine hematopoietic progenitor cells were characterized by separation with monoclonal antibodies. Depleted and enriched fractions were studied for growth of CFU-GM in semisolid agar and for repopulating capacity of lethally irradiated dogs. CFU growth was not reduced by depletion of marrow using monoclonal antibodies F 3-20-7 (anti-dog Thy-1), MT606 (anti-human CD 6), and IOT2a (anti-human DR). CFU growth was variable following treatment with the anti-canine T-cell antibody MdT-P 1 and immunomagnetic bead separation. It was regularly enriched when MdT-P 1 treatment was followed by immunorosetting with staphylococcal protein A-loaded sheep red blood cells and density gradient separation. Lethally irradiated dogs were reconstituted by autologous marrow depleted of MdT-P 1-positive cells using immunorosetting and density gradient centrifugation, whereas immunomagnetic bead-depleted marrow was ineffective. Fluorescence-activated cell sorting showed enrichment of hematopoietic progenitor cells in the weakly MdT-P 1-positive fraction.
Hahn
J
J
Institut für Immunologie, Hämatologikum der GSF, München, FRG.
Kolb
H J
HJ
Schumm
M
M
Beisser
K
K
Ellwart
J
J
Rieber
P
P
Maldacker
J
J
Schwella
N
N
Lösslein
L K
LK
Holler
E
E
D000818:Animals; D000911:Antibodies, Monoclonal; D016026:Bone Marrow Transplantation / Q000276:immunology / Q000662:veterinary; D002469:Cell Separation; D003114:Colony-Forming Units Assay; D003429:Cross Reactions; D004285:Dogs / Q000097:blood* / Q000276:immunology; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000276:immunology* / Q000502:physiology
D000911:Antibodies, Monoclonal