Identification of self peptides bound to purified HLA-B27.
A pool of endogenous peptides bound to the human class I MHC molecule, HLA-B27, has been isolated. Microsequence analysis of the pool and of 11 HPLC-purified peptides provides information on the binding specificity of the HLA-B27 molecule. The peptides all seem to be nonamers, seven of which match to protein sequences in a database search. These self peptides derive from abundant cytosolic or nuclear proteins, such as histone, ribosomal proteins, and members of the 90K heat-shock protein family.
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
T S
TS
Jardetzky
W S
WS
Lane
R A
RA
Robinson
D R
DR
Madden
D C
DC
Wiley
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015796:HLA-B27 Antigen / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D012689:Sequence Homology, Nucleic Acid
D015796:HLA-B27 Antigen; D010455:Peptides