Convenient agarose medium for simultaneous determination of the low-calcium response and Congo red binding by virulent strains of Yersinia enterocolitica.
A simple, efficient method for identification and differentiation of Yersinia enterocolitica containing virulent plasmid-bearing clones is described. The method is based on temperature of incubation and the low calcium response of the organism in an agarose Congo red medium.
Bhaduri
S
S
U.S. Department of Agriculture, Agricultural Research Service, Philadelphia, Pennsylvania 19118.
Turner-Jones
C
C
Lachica
R V
RV
D002118:Calcium / Q000494:pharmacology; D003224:Congo Red; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D010957:Plasmids; D012703:Serotyping; D014774:Virulence / Q000235:genetics; D015008:Yersinia enterocolitica / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*
D004269:DNA, Bacterial; D003224:Congo Red; D002118:Calcium