Monoclonal antibodies differentiate between the haemagglutinating and the receptor-destroying activities of bovine coronavirus.
A relatively simple and sensitive method is described which enables the effect of monoclonal antibodies (MAbs) on the receptor-destroying enzyme (RDE) and the haemagglutination (HA) activity of bovine coronavirus (BCV) to be analysed in one assay. A lysate of HRT-18 cells infected with the L9 strain of BCV was found to have a higher RDE:HA ratio than purified virus. At 4 degrees C the lysate induced an HA pattern which completely disappeared upon raising of the temperature to 37 degrees C. This L9-infected cell lysate was used to determine the HA inhibition (HAI) titres of MAbs directed against the surface glycoproteins S and HE of BCV. Thereafter, the test plates were incubated at 37 degrees C to enable the ability of the MAbs to prevent elution of virus from BCV-erythrocyte complexes to be assessed. No inhibition of RDE was detectable with MAbs against glycoprotein S, which had HAI titres ranging from 1:16 to 1:128. On the other hand, MAbs directed against glycoprotein HE had similar HAI titres, but they inhibited elution of 8 HA units of BCV at titres of up to 1:65,000.
Department of Veterinary Microbiology and Parasitology, Louisiana State University, Baton Rouge.
J
J
Storz
G
G
Herrler
D R
DR
Snodgrass
K A
KA
Hussain
X M
XM
Zhang
M A
MA
Clark
R
R
Rott
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002645:Chickens; D003332:Coronaviridae / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism / Q000382:microbiology; D006385:Hemagglutination Inhibition Tests; D006388:Hemagglutinins / Q000378:metabolism*; D006389:Hemagglutinins, Viral / Q000378:metabolism*; D011991:Receptors, Virus / Q000276:immunology / Q000378:metabolism*; D012794:Sialic Acids / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000276:immunology / Q000378:metabolism*; D014760:Viral Fusion Proteins; D014764:Viral Proteins / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D006388:Hemagglutinins; D006389:Hemagglutinins, Viral; D011991:Receptors, Virus; D012794:Sialic Acids; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; C060814:hemagglutinin esterase; C025676:9-O-acetyl-N-acetylneuraminic acid