Techniques used to define human MHC antigens: monoclonals, class I and class II biochemistry.
A variety of straightforward biochemical techniques have been applied to the analysis of HLA molecules. These techniques centre largely on two components: monoclonal antibodies (mAbs) and gel analysis systems. Although only the basic applications are discussed here, the techniques have broad applications. Monoclonal-gel systems may be used for the characterisation of polymorphisms in HLA class I alpha chains or class II alpha and beta chains. This requires the use of mAbs which are locus-specific and monomorphic. Alternatively, B lymphoblastoid cell lines (B-LCL) whose HLA antigen mobilities are known in advance may be used for screening uncharacterised monoclonals for locus specificity.
Molecular Immunogenetics Units, UMDS, Guy's Hospital, London, U.K.
J S
JS
Lanchbury
D000483:Alleles; D000911:Antibodies, Monoclonal; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006680:HLA Antigens / Q000032:analysis* / Q000235:genetics; D006681:HLA-D Antigens / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007525:Isoelectric Focusing; D011110:Polymorphism, Genetic
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D006681:HLA-D Antigens