Expression of rat hepatic lipase in heterologous systems: evidence for different sites for interface binding and catalysis.
Rat hepatic triglyceride lipase was expressed as a bacterial fusion protein and as a secreted protein in eukaryotic cells. The bacterial fusion construct coded for seven amino acids at the N-terminus which are not present in the hepatic lipase cDNA, but otherwise consisted of only the complete mature lipase sequence. Fusion protein was isolated as an insoluble product which did not have lipase or phospholipase activities; it was, however, active as an esterase when solubilized after preparative gel electrophoresis. The fusion protein was used to raise polyclonal antibodies that recognize native rat hepatic lipase and inhibit its activity. For eukaryotic expression, a full-length rat hepatic lipase cDNA clone was inserted into the metallothionein promoter expression vector pMTSV40polyA.Bam. Transfected CHO cells, induced with ZnSO4, secreted an immunoreactive protein of Mr approximately 57,000. A lipase-producing clonal cell line was isolated and used to characterize the enzyme. The protein was purified from serum-free medium by heparin-Sepharose and DEAE-Trisacryl M column chromatography. It was apparently identical to native rat hepatic lipase, with the exception of the conformation of the linkage of the sialic acids which form part of the N-linked carbohydrate complexes. The bacterial fusion protein, the CHO-produced lipase, and native rat hepatic lipase were all inhibited by phenylmethylsulfonyl fluoride, implying that they function catalytically as serine esterases. Substrate competition studies indicated that the esterase and lipase activities use the same active site; thus, the major defect in the fusion protein was probably in triglyceride substrate binding. These results suggest that interface binding and catalysis occur at different sites in the protein.
Komaromy
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MC
Research Institute, Palo Alto Medical Foundation, CA 94301.
Reed
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D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism; D001665:Binding Sites; D015152:Blotting, Northern; D002384:Catalysis; D002469:Cell Separation; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004950:Esterases / Q000378:metabolism; D007106:Immune Sera / Q000096:biosynthesis; D008049:Lipase / Q000096:biosynthesis / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism
D001426:Bacterial Proteins; D007106:Immune Sera; D011993:Recombinant Fusion Proteins; D004950:Esterases; D008049:Lipase