Induction of NF-kappa B DNA-binding activity during the G0-to-G1 transition in mouse fibroblasts.
A DNA-binding factor with properties of NF-kappa B and another similar activity are rapidly induced when growth-arrested BALB/c 3T3 cells are stimulated with serum growth factors. Induction of these DNA-binding activities is not inhibited by pretreatment of quiescent cells with the protein synthesis inhibitor cycloheximide. Interestingly, the major NF-kappa B-like activity is not detected in nuclear extracts of proliferating cells, and thus its expression appears to be limited to the G0-to-G1 transition in 3T3 cells. These DNA-binding activities bind many of the expected NF-kappa B target sequences, including elements in the class I major histocompatibility complex and human immunodeficiency virus enhancers, as well as a recently identified NF-kappa B binding site upstream of the c-myc gene. Furthermore, both the class I major histocompatibility complex and c-myc NF-kappa B binding sites confer inducibility on a minimal promoter in 3T3 cells stimulated with serum growth factors. The results demonstrate that NF-kappa B-like activities are immediate-early response proteins in 3T3 cells and suggest a role for these factors in the G0-to-G1 transition.
Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill 27599-7295.
A S
AS
Baldwin
J C
JC
Azizkhan
D E
DE
Jensen
A A
AA
Beg
L R
LR
Coodly
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D005347:Fibroblasts; D016193:G1 Phase; D005786:Gene Expression Regulation / Q000187:drug effects*; D005805:Genes, MHC Class I; D016259:Genes, myc; D006133:Growth Substances / Q000494:pharmacology*; D016322:HIV Enhancer / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D016192:Resting Phase, Cell Cycle
D006133:Growth Substances; D016328:NF-kappa B