A low molecular weight endometrial secretory protein which is increased by ovine trophoblast protein-1 is a beta 2-microglobulin-like protein.
Ovine trophoblast protein-1 (oTP-1), stimulates the secretion of several proteins in explant culture of day-12 cyclic ovine endometrium. We partially purified and identified one of these proteins, an 11,000 Mr, pI approx. 6 protein by N-terminal amino acid sequencing and immunoprecipitation using antibody to human beta 2-microglobulin. The protein was purified from cultures of endometrium collected from day-16 pregnant ewes. The N-terminal amino acid sequence was 40-55% homologous to beta 2-microglobulin from a variety of species. Antibody to human beta 2-microglobulin immunoprecipitated the protein and another protein of similar molecular weight but more acidic pI. Using immunoprecipitation of radiolabelled proteins from culture, we demonstrated that oTP-1 increased production of this protein by 40% (P less than 0.05). We conclude that oTP-1 increases the secretion of a beta 2-microglobulin-like protein from day-12 non-pregnant endometrium in culture.
AFRC Research Group on Hormones and Farm Animal Reproduction, University of Nottingham, Bonington, Leicester.
J L
JL
Vallet
P J
PJ
Barker
G E
GE
Lamming
N
N
Skinner
N S
NS
Huskisson
D000595:Amino Acid Sequence; D000818:Animals; D046508:Culture Techniques; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D007370:Interferon Type I / Q000494:pharmacology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000494:pharmacology*; D011270:Pregnancy, Animal / Q000502:physiology*; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep / Q000502:physiology*; D013268:Stimulation, Chemical; D001613:beta 2-Microglobulin / Q000235:genetics / Q000378:metabolism*
D007370:Interferon Type I; D011257:Pregnancy Proteins; D001613:beta 2-Microglobulin; C020947:interferon tau