Analysis of flucytosine dosage forms by derivative UV spectroscopy and liquid chromatography.
A simple second-order derivative spectrophotometric method was developed for the selective determination of flucytosine (an antimycotic drug) in the presence of 5-fluorouracil (a cytotoxic agent), its synthetic precursor and degradation product. Traces of 5-fluorouracil in flucytosine were also determined by derivative UV spectroscopy; flucytosine was removed by a selective solid-phase extraction (SPE) procedure using a strong cation-exchange sorbent. The spectrophotometric methods were applied successfully to the quality control of commercial dosage forms of flucytosine and the results were compared with those obtained by a HPLC procedure (cyano column) developed as a reference method.
Department of Pharmaceutical Sciences, University of Bologna, Italy.
V
V
Cavrini
D
D
Bonazzi
A M
AM
Di Pietra
D002851:Chromatography, High Pressure Liquid; D004304:Dosage Forms; D005437:Flucytosine / Q000032:analysis*; D005472:Fluorouracil / Q000032:analysis*; D011786:Quality Control; D013056:Spectrophotometry, Ultraviolet
D004304:Dosage Forms; D005437:Flucytosine; D005472:Fluorouracil