Effects of polyene macrolides on the membrane potential of resting and activated human leukocytes.
Membrane potential changes in human polymorphonuclear leukocytes have been studied by monitoring the fluorescence signal of a carbocyanine probe in the presence of calcium ionophore A23187, chemotactic tripeptide f-met-leu-phe, and polyene macrolides. Reduction of the signals triggered by the ionophore and the tripeptide, inhibition of the respiratory burst, and changes in the Na+ influx into the cell seem to be the dominant effect of the polyenes. Polyene-induced depolarization does not correlate with the inhibition of the respiratory burst.
Department of Biochemical Sciences, University of Rome La Sapienza, Italy.
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Jullien
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Capuozzo
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Salerno
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Crifò
D000666:Amphotericin B / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D002794:Choline / Q000494:pharmacology; D005454:Fluorescence Polarization; D006801:Humans; D008165:Luminol / Q000737:chemistry; D008564:Membrane Potentials / Q000187:drug effects*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D000001:Calcimycin; D012965:Sodium Chloride; D009240:N-Formylmethionine Leucyl-Phenylalanine; D008165:Luminol; D000666:Amphotericin B; D002794:Choline