Lack of specific hybridization between the lep genes of Salmonella typhimurium and Bacillus licheniformis.
This paper describes an attempt to clone the Bacillus licheniformis lep gene, encoding signal peptidase, using the Salmonella typhimurium lep gene as a hybridization probe. Although a hybridizing fragment was obtained, DNA sequence analysis indicated that it did not contain the lep gene. Instead, the protein encoded by the cloned fragment showed similarity with a variety of L-asparaginases.
Department of Genetics, Center of Biological Sciences, Haren, The Netherlands.
J M
JM
van Dijl
A
A
de Jong
H
H
Smith
S
S
Bron
G
G
Venema
D000595:Amino Acid Sequence; D001215:Asparaginase / Q000235:genetics; D001407:Bacillus / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes / Q000235:genetics; D010450:Endopeptidases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000235:genetics*; D012697:Serine Endopeptidases
D015342:DNA Probes; D008565:Membrane Proteins; D010450:Endopeptidases; D012697:Serine Endopeptidases; C026515:type I signal peptidase; D001215:Asparaginase