[Aeromonas spp. isolated from human feces. Species and pathogenicity factors].
The role of Aeromonas genus as a primary intestinal pathogen is still a matter of controversy. We have studied the isolation of Aeromonas spp. from human stools samples, looking for the production of pathogenic factors and its relationship with different species. From a total of 471 strains isolated, 241 were A. caviae, 127 A. sobria, 89 A. hydrophila and 14 non-typable strains. The pathogenic factors production was studied in a bacteria-free filtrate from a 18 hours trypticase-soy broth culture. Enterotoxin production was assessed by suckling-mice test, cytotoxin production by using Hela cells monolayer and hemolysin production by double dilution from the filtrate, plated in microdilution plates, against rabbit red-blood cells. Enterotoxin production test was positive for A. sobria, A. hydrophila and A. caviae in 81.1%, 62.9% and 7.0% of strains respectively. Cytotoxin production was also positive for these species in 96.6%, 76.4% and 4.1%, and hemolysin production was positive in 95.3%, 70.7% and 2.4% of the strains respectively.
Servicio de Microbiologia y Parasitologia, Hospital La Fe, Valencia.
B
B
Acosta
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Pérez de León
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M
Gobernado
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A
Camañas
D000333:Aeromonas / Q000302:isolation & purification* / Q000378:metabolism / Q000472:pathogenicity; D000818:Animals; D003603:Cytotoxins / Q000096:biosynthesis; D004768:Enterotoxins / Q000096:biosynthesis; D005243:Feces / Q000382:microbiology*; D005767:Gastrointestinal Diseases / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006460:Hemolysin Proteins / Q000096:biosynthesis; D006801:Humans; D051379:Mice; D013045:Species Specificity
D003603:Cytotoxins; D004768:Enterotoxins; D006460:Hemolysin Proteins
Aeromonas spp. aisladas de heces humanas. Especie y factores de patogenicidad.