Studies on lipid peroxidation in pancreatic tissue. In vitro formation of thiobarbituric-acid-reactive substances (TBRS).
In pathogenic studies on acute pancreatitis the importance of a temporary ischemia on induction of autodigestion was demonstrated. Because of the involvement of oxygen-derived free radicals in the ischemia/reperfusion injury of other tissues we have investigated the influence of artificial oxidants, as FeCl3 and H2O2, on pancreatic tissue and isolated pancreatic acinar cells. Lipid peroxidation was determined as thiobarbituric-acid-reactive substances (TBRS). In these experiments the TBRS concentration was elevated within the first min of incubation with FeCl3. The exposure of pancreas homogenates and intact acinar cells to H2O2 had no remarkable effect on formation of TBRS. Under this condition the survival of cells was strongly reduced, while cells exposed to FeCl3 revealed a remarkably slower rate of cytolysis. The missing correlation between cell lysis and elevation of TBRS suggests that lipid peroxidation might not be essential process in pancreatic acinar cell damage.
Letko
G
G
Medical Academy of Magdeburg, Clinic of Surgery, F.R.G.
Winkler
U
U
Matthias
R
R
Heinrich
P
P
D000818:Animals; D002478:Cells, Cultured; D002712:Chlorides; D004305:Dose-Response Relationship, Drug; D005260:Female; D005290:Ferric Compounds / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D010179:Pancreas / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D013858:Thiobarbiturates / Q000378:metabolism*
D002712:Chlorides; D005290:Ferric Compounds; D013858:Thiobarbiturates; D006861:Hydrogen Peroxide; C029684:thiobarbituric acid; C024555:ferric chloride