Cloning of fish zinc-finger genes related to Krox-20 and Krox-24.
Southern hybridization suggests that the zebrafish genome contains multiple zinc-finger genes related to the putative mouse developmental genes, Krox-20 and Krox-24. The polymerase chain reaction was employed to amplify and clone the zinc-finger regions of genes related to Krox-20, from two fish species and, for comparison, mouse, hamster and fox. DNA sequence analyses suggest that the genes cloned include the guppy homologue of Krox-20 and the zebrafish homologue of Krox-24, and that these genes diverged prior to the separation of the lineages leading to teleosts and to mammals.
Department of Zoology, University of Oxford, U.K.
J
J
Lanfear
T
T
Jowett
P W
PW
Holland
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005399:Fishes / Q000235:genetics*; D005796:Genes; D008099:Liver / Q000502:physiology; D051379:Mice; D008811:Mice, Inbred DBA / Q000235:genetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011036:Poecilia / Q000235:genetics; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D015027:Zebrafish / Q000235:genetics; D016335:Zinc Fingers / Q000235:genetics*
D009838:Oligodeoxyribonucleotides; D004247:DNA