RecA-mediated annealing of single-stranded DNA and its relation to the mechanism of homologous recombination.
We demonstrate that RecA protein can mediate annealing of complementary DNA strands in vitro by at least two different mechanisms. The first annealing mechanism predominates under conditions where RecA protein causes coaggregation of single-stranded DNA (ssDNA) molecules and where RecA-free ssDNA stretches are present on both reaction partners. Under these conditions annealing can take place between locally concentrated protein-free complementary sequences. Other DNA aggregating agents like histone H1 or ethanol stimulate annealing by the same mechanism. The second mechanism of RecA-mediated annealing of complementary DNA strands is best manifested when preformed saturated RecA-ssDNA complexes interact with protein-free ssDNA. In this case, annealing can occur between the ssDNA strand resident in the complex and the ssDNA strand that interacts with the preformed RecA-ssDNA complex. Here, the action of RecA protein reflects its specific recombination promoting mechanism. This mechanism enables DNA molecules resident in the presynaptic RecA-DNA complexes to be exposed for hydrogen bond formation with DNA molecules contacting the presynaptic RecA-DNA filament.
Institut für Zellbiologie, ETH-Zürich, Switzerland.
B
B
Müller
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A
Stasiak
D000255:Adenosine Triphosphate / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism* / Q000648:ultrastructure; D004587:Electrophoresis, Agar Gel; D007700:Kinetics; D008854:Microscopy, Electron; D011938:Rec A Recombinases / Q000378:metabolism* / Q000648:ultrastructure; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid
D004277:DNA, Single-Stranded; D000255:Adenosine Triphosphate; D011938:Rec A Recombinases