Turkey gizzard caldesmon: complete sequence of a C-terminal thrombic fragment that binds actin, tropomyosin and calmodulin.
We have determined, by protein chemistry methods, the complete amino acid sequence of a thrombic fragment, "CaD35", which contains the C-terminal 274 residues of turkey gizzard caldesmon. Residues 1-96 of CaD35 comprise an actin-binding subfragment which resembles the tropomyosin-binding segment of troponin T. Residues 111-128 and 255-272 may form basic, amphipathic helices that interact with calmodulin. Residues 251-253 (Ser-Ser-Ser) may be phosphorylated. Chymotryptic CaD35, obtained from chicken gizzard caldesmon, nearly identical in sequence to the turkey gizzard fragment, but contains an additional 32 amino acid residues at its N terminus.
Department of Biological Chemistry, School of Medicine, University of Maryland, Baltimore 21201.
J H
JH
Collins
J
J
Leszyk
D
D
Mornet
E
E
Audemard
D000199:Actins / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D002147:Calmodulin / Q000737:chemistry*; D002148:Calmodulin-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification; D005895:Gizzard, Avian / Q000737:chemistry*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000302:isolation & purification; D011485:Protein Binding; D013917:Thrombin; D014335:Tropomyosin / Q000737:chemistry*; D014422:Turkeys
D000199:Actins; D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D010446:Peptide Fragments; D014335:Tropomyosin; D013917:Thrombin