Characterization of pollen polygalacturonase encoded by several cDNA clones in maize.
A full-length cDNA clone, named PG1, abundantly expressed in late stages of pollen development, has been isolated from a cDNA library using a differential screening method with cDNA probes representative of microspores at early or late developmental stages. The encoded 410 amino acid polypeptide has significant homology with various polygalacturonases (PG) described elsewhere. Two polypeptides, of 49 and 53 kDa respectively, have been identified in the active PG fraction, isolated from mature pollen by immuno-cross-reaction with tomato PG antibodies. According to their N-terminal sequence, they can be identified as being mature peptides encoded by the PG1 cDNA clone. We propose that these two proteins derive from a unique precursor through several post-translational events, including the excision of a 22 amino-terminal signal peptide and glycosylation. PG-encoding genes from a small genomic family. Sequence analysis of three PG cDNA clones shows that they are closely related. The divergence of nucleotides between these three cDNA clones is 1%. They encode the same product.
Laboratoire de Biologie Moléculaire végétale, CNRS, Grenoble, France.
M F
MF
Niogret
M
M
Dubald
P
P
Mandaron
R
R
Mache
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D006031:Glycosylation; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011058:Pollen / Q000201:enzymology* / Q000235:genetics; D011096:Polygalacturonase / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000378:metabolism; D015183:Restriction Mapping; D016415:Sequence Alignment; D013170:Spores / Q000254:growth & development; D003313:Zea mays / Q000201:enzymology / Q000235:genetics*
D010455:Peptides; D010940:Plant Proteins; D011498:Protein Precursors; D012333:RNA, Messenger; D004247:DNA; D011096:Polygalacturonase