High-performance liquid chromatographic determination of histamine N-methyltransferase activity.
A method for the determination of histamine N-methyltransferase (HMT) activity by high-performance liquid chromatography based on post-column derivatization with omicron-phthalaldehyde is described. The determination involves the separation of the substrate, histamine, from its product. N tau-methylhistamine, using a weak cation exchanger, followed by on-line derivatization of these imidazoleamines with omicron-phthalaldehyde and their detection and quantitation with a fluorimetric detector. This assay method is suitable for the measurement of HMT activity during enzyme purification.
Department of Pharmacology II, Osaka University, Faculty of Medicine, Japan.
H
H
Fukuda
A
A
Yamatodani
I
I
Imamura
K
K
Maeyama
T
T
Watanabe
H
H
Wada
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006632:Histamine / Q000032:analysis / Q000378:metabolism; D006637:Histamine N-Methyltransferase / Q000032:analysis* / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008297:Male; D008761:Methylhistamines / Q000032:analysis / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence; D009764:o-Phthalaldehyde
D008761:Methylhistamines; D009764:o-Phthalaldehyde; C031205:3-methylhistamine; D006632:Histamine; D006637:Histamine N-Methyltransferase