New method for isolating and quantifying intermediate and beta-very-low-density lipoprotein cholesterol.
We describe a new method, useful to clinical laboratories, for assessing intermediate density (IDL) or beta-very-low-density (beta-VLDL) lipoprotein cholesterol. The technique involves selective precipitation properties of the qualitative Wieland and Seidel post-electrophoretic method that immobilizes IDL and beta-VLDL in the beta-zone of an agarose slide (Clin Chem 1973;19:1139-41). In our method, we separate low-density lipoprotein (LDL) in a second electrophoretic step, in which LDL moves toward the anode, and then quantify the cholesterol of the above lipoproteins remaining in the precipitate band at the beta-zone. Replicate within-run precision (CV) of 15 aliquots of a sera pool was 10.1%. The correlation with sequential ultracentrifugation of 30 samples was r = 0.96 (P less than 0.001). Serum reference values for 30 normal individuals are 57 +/- 7.0 mg/L. Seven phenotype III hyperlipoproteinemic patients had the highest concentrations of IDL or beta-VLDL cholesterol in serum, 1620 +/- 346 mg/L.
Department of Clinical Biochemistry, University of Buenos Aires, Argentina.
R L
RL
Wikinski
L E
LE
Schreier
S B
SB
Rosental
D011232:Chemical Precipitation; D002784:Cholesterol / Q000097:blood; D015243:Cholesterol, VLDL / Q000097:blood*; D004587:Electrophoresis, Agar Gel / Q000706:statistics & numerical data; D006801:Humans; D006952:Hyperlipoproteinemia Type III / Q000097:blood*; D008074:Lipoproteins / Q000097:blood; D012016:Reference Values; D014280:Triglycerides / Q000097:blood; D014461:Ultracentrifugation
D015243:Cholesterol, VLDL; D008074:Lipoproteins; D014280:Triglycerides; C030104:lipoprotein cholesterol; D002784:Cholesterol