Dihydrofolate reductase gene expression characterized by the PCR assay in human leukemia cells.
A modified enzymatic amplification (PCR) method has been used to detect overexpression of the dihydrofolate reductase gene in human leukemic cells resistant to methotrexate (MTX) from both patient samples and cell lines in culture. Quantitation of DHFR mRNA by the PCR assay has been demonstrated to be more accurate and more sensitive than Northern blotting. This assay be useful in screening patients relapsing with drug resistance.
Department of Medical Oncology, City of Hope National Medical Center, Duarte, CA 91010.
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Miyachi
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Han
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Scanlon
D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D004351:Drug Resistance; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D007938:Leukemia / Q000188:drug therapy / Q000201:enzymology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis; D012680:Sensitivity and Specificity; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
D012333:RNA, Messenger; D013762:Tetrahydrofolate Dehydrogenase