Development of enucleated mouse oocytes reconstituted with embryonic nuclei.
The chromosomes of mouse oocytes at telophase of the first meiotic division were removed using micromanipulation and differential interference microscopy. The enucleated oocytes were used as recipients for nuclear transplantation, after culture for 4-6 h. The newly synthesized proteins of the enucleated oocytes showed the same pattern as those of secondary oocytes matured in vivo. When the enucleated oocytes received a nucleus from late 2- and 8-cell embryos, or a cell from the inner cell mass (ICM) of blastocysts, 23, 4 and 10%, respectively, of reconstituted embryos developed to blastocysts. After transfer to recipient females, live young were produced from the reconstituted eggs that received a nucleus from late 2-cell embryos.
NODAI Research Institute, Tokyo University of Agriculture, Japan.
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Kono
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Kwon
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Nakahara
D000705:Anaphase; D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D002875:Chromosomes; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D008846:Micromanipulation / Q000379:methods; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D011247:Pregnancy; D014176:Protein Biosynthesis