Effect of media conditioned by concanavalin A activated spleen cells on pancreatic islet cells.
The effect of media conditioned by concanavalin A-activated spleen cells (C-sup) on insulin release and its islet cell cytotoxicity were studied. In a functional study, C-sup significantly inhibited both basal insulin release and glucose-stimulated insulin release. Morphologically, C-sup had a destructive effect on isolated islets after 72 h incubation. Islet cell cytotoxicity was shown by lactate dehydrogenase (LDH) release assay after 5 days incubation with C-sup in a dose-dependent manner. These results suggest that acceleration of the onset of diabetes in young diabetes prone (DP) Bio-Breeding/Worcester (BB/W) rats following the injection of C-sup may depend on the suppressive and cytotoxic effects of C-sup on pancreatic islet cells.
Sawada
M
M
First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
Nakano
K
K
Hasegawa
G
G
Setoguchi
J
J
Shigeta
H
H
Nakamura
N
N
Kanatsuna
T
T
Kondo
M
M
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A; D003602:Cytotoxicity, Immunologic; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D007515:Islets of Langerhans / Q000276:immunology* / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D007328:Insulin; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; D007371:Interferon-gamma; D007770:L-Lactate Dehydrogenase