Target cell membrane modification and susceptibility to lymphokine activated killer cell mediated lysis. I. Trinitrophenyl has no effect on murine normal cells and tumors.
Lymphokine activated killer (LAK) cells selectively recognize and lyse a wide variety of tumor cells, including syngeneic, allogeneic, and xenogeneic cells, by a mechanism which remains unknown. It has been shown that trinitrophenyl modification of human peripheral blood lymphocytes (PBLs) and murine blasts induces sensitivity to lysis by LAK cells. We undertook these studies to examine the effects of trinitrophenyl (TNP) on murine normal cells and tumors. Fresh murine PBLs are resistant to LAK mediated lysis and TNP modification of the cell surface has no effect, in direct contrast to results with human cells. However, the addition of anti-TNP antibody demonstrated lysis of the TNP modified cells by an antibody dependent cellular toxicity mechanism showing that these cells can be lysed by LAK cells. Furthermore, TNP did not induce sensitivity to LAK cell mediated lysis on other normal murine tissues including kidney and bone marrow. The lysis of YAC tumor cells is not inhibited by the addition of TNP modified PBLs. A single cell assay demonstrated no conjugation of LAK effector cells and TNP modified PBLs. LAK cells derived from murine PBLs, as is done in the human, were unable to lyse the TNP modified PBLs, ruling out the origin of the LAK cells as the reason for the different results in humans and mice. These observations represent a difference in the spectrum of target cells lysed by human and murine LAK cells, and also demonstrate a response to TNP modification by fresh murine normal tissues and tumors that differs from that observed with murine blasts.(ABSTRACT TRUNCATED AT 250 WORDS)
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Department of Surgery, University of Maryland, Baltimore 21201.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000276:immunology; D002462:Cell Membrane / Q000187:drug effects / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D005260:Female; D005354:Fibrosarcoma; D007668:Kidney / Q000276:immunology; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008223:Lymphoma; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology*; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology*
D014302:Trinitrobenzenesulfonic Acid