Overproduction, purification and crystallization of Bacillus cereus oligo-1,6-glucosidase.
The gene coding for oligo-1,6-glucosidase from Bacillus cereus ATCC7064 has been overexpressed in Escherichia coli MV1184 cells under the control of the lac promoter in the genetically engineered plasmid pBCE4-2. Oligo-1,6-glucosidase was purified in large quantities and was crystallized at 25 degrees C by using a hanging drop vapor diffusion method with 53% saturated ammonium sulfate. The crystals have the shape of hexagonal bipyramids and belong to the space group P6(2) or P6(4) with lattice constants of a = b = 106.1 A, c = 120.0 A and gamma = 120 degrees.
Watanabe
K
K
Department of Agricultural Chemistry, Kyoto Prefectural University, Japan.
Kitamura
K
K
Hata
Y
Y
Katsube
Y
Y
Suzuki
Y
Y
D001409:Bacillus cereus / Q000201:enzymology*; D003461:Crystallography; D009834:Oligo-1,6-Glucosidase / Q000737:chemistry / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000302:isolation & purification
D011994:Recombinant Proteins; D009834:Oligo-1,6-Glucosidase