Ligand-dependent aggregation and cooperativity: a critique.
Ligand-dependent site-site (or subunit-subunit) interactions provide the basis for explaining cooperativity in chemical reactions. Even in the simplest possible nonaggregating system, interpretation of the interactions in terms of structural details requires an explicit assumption (or model) for the binding of the ligand to the sites when there are no interactions. This paper develops in detail the processes by which aggregation will yield ligand-dependent cooperativity. Two conceptually distinct free energy differences may contribute to cooperativity in an aggregation reaction. One is the free energy difference in ligand binding between the monomer and the aggregate. The other is derived from ligand-dependent interactions between the sites of the aggregate. In this analysis an explicit distinction is made between the experimentally accessible constants and those derived from assumed models. Experimental measurements of an aggregation cycle in which all of the species in equilibrium are defined do not allow for an evaluation of the energies of interaction without some model (or assumption). In the analysis presented, an explicit assumption is employed relating the constant for binding of the ligand to the isolated monomer and the constant for the binding of the ligand to aggregate under conditions where there are no ligand-dependent interactions.
Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
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Saroff
D008024:Ligands; D011485:Protein Binding; D013329:Structure-Activity Relationship; D013816:Thermodynamics
D008024:Ligands