Characterization of the hydrophobic region of heat shock protein 90.
The modes of binding of heat shock protein 90 with phenyl-Sepharose, myristoylated AE-cellulose, and monomyristoylated lysozyme were studied to characterize a hydrophobic region(s) on the surface of the heat shock protein 90 molecule and the following results were obtained. (1) The binding of heat shock protein 90 with phenyl-Sepharose was inhibited by the addition of 30% ethylene glycol. This indicates that the binding involves a hydrophobic interaction. (2) The binding was strengthened by the addition of 10 mM Mg2+, Ca2+, Sr2+, and Ba2+ ions, but not by K+ or Na+ ions. (3) The binding of hsp 90 with phenyl-Sepharose decreased initially and then increased as the temperature was increased from 0 to 50 degrees C, with a minimum at around 35 degrees C. (4) Lowering the pH stimulated the binding of hsp 90 with phenyl-Sepharose. (5) Heat shock protein 90 bound to myristoylated AE-cellulose, which has aliphatic hydrophobic residues, but not to acetylated AE-cellulose. (6) Heat shock protein 90 bound to monomyristoylated lysozyme, but not to control unmodified lysozyme. Based on these results, the possible function of the hydrophobic region(s) of heat shock protein 90 in the interaction with hydrophobic proteins is discussed.
Yamamoto
M
M
Department of Biochemistry, Faculty of Science, Niigata University.
Takahashi
Y
Y
Inano
K
K
Horigome
T
T
Sugano
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002413:Cations, Divalent; D002482:Cellulose / Q000031:analogs & derivatives; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009113:Muramidase; D009227:Myristic Acids; D051381:Rats; D012685:Sepharose / Q000031:analogs & derivatives; D013696:Temperature
D002413:Cations, Divalent; D006360:Heat-Shock Proteins; D009227:Myristic Acids; C020927:phenyl-sepharose; D002482:Cellulose; D012685:Sepharose; C010021:aminoethyl cellulose; D009113:Muramidase