Responsiveness of the increase in C-fos mRNA levels depends on the inducer and the cell's past.
In this paper we show that in C3H10T1/2 mouse fibroblasts, the inducibility of c-fos mRNA by heat shock or serum addition is strongly dependent on the cell's past. Four hours after a heat shock, a time point where the induced c-fos mRNA has disappeared, c-fos mRNA could not be induced again by a second heat shock. Four hours after serum addition, by which c-fos was induced, a second serum addition also failed to induce c-fos mRNA again. When, however, serum was added 4 hours after heat shock or heat shock was given 4 hours after serum addition, levels of c-fos mRNA could be enhanced again. The induction by serum of c-fos mRNA levels in thermotolerant cells might be related to their increased stimulation of DNA synthesis as compared to control cells.
Department of Molecular Cell Biology, University of Utrecht, The Netherlands.
M J
MJ
Tuijl
J A
JA
Den Boon
W M
WM
Van Grunsven
R
R
Van Wijk
D000818:Animals; D001769:Blood; D002460:Cell Line; D003470:Culture Media; D005786:Gene Expression Regulation; D016762:Genes, fos; D006358:Hot Temperature; D007399:Interphase; D051379:Mice; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism
D003470:Culture Media; D012333:RNA, Messenger