Nutritional value of yeast for Dermatophagoides pteronyssinus (Acari: Epidermoptidae) and the antigenic and allergenic composition of extracts during extended culturing.
Cultures of European house dust mite, Dermatophagoides pteronyssinus (Trouessart), were evaluated with respect to yeast nutritional value, together with an assessment of the antigenic and allergenic composition. CIE analysis of extracts illustrated that yeast contributes many antigens throughout the rearing period. However, there is an indication of the consumption of only one particular yeast antigen by the mites. Total protein content profile of the mite cultures is shown in addition to the total allergen activity (RAST determinations). The allergenic activity of the extracts steadily increased during the mite culturing period; this may be due to the accumulation of fecal particles containing the major allergen.
Andersen
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Institute I, Life Sciences and Chemistry, Roskilde University, Denmark.
D000485:Allergens / Q000032:analysis*; D000818:Animals; D000941:Antigens / Q000032:analysis*; D003470:Culture Media; D004391:Dust; D008925:Mites / Q000254:growth & development* / Q000276:immunology; D009753:Nutritive Value; D012441:Saccharomyces cerevisiae / Q000502:physiology*
D000485:Allergens; D000941:Antigens; D003470:Culture Media; D004391:Dust