[Determining the levels of staphylococcal enterotoxins types A and B in food products by the immunoenzyme method].
"Sandwich" variant of ELISA was used to identify staphylococcal enterotoxins (SE), types A and B, in S. aureus filtrates inducing food poisoning, in extracts of the lactic acid product for infants "Biphilin" that caused staphylococcal infection, and in foods contaminated with SE in varying concentrations. It has been shown that ELISA used for SE identification in foods permits revealing SE, types A and B, in liquid products in concentrations of 1-2 ng/ml (that is 1000-fold more sensitive, than the immunodiffusion test, 400-800-fold more sensitive than the passive hemagglutination test, and 10-fold more sensitive than the indirect passive hemagglutination test), and in solid products--in concentrations of 5-10 ng/g (after artificial contamination).
Fluer
F S
FS
Mikheeva
G V
GV
Akatov
A K
AK
Kuvaeva
I B
IB
Sheveleva
S A
SA
Kolosnitsina
N V
NV
D000818:Animals; D003611:Dairy Products / Q000032:analysis* / Q000592:standards; D004768:Enterotoxins / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005516:Food Microbiology / Q000592:standards*; D006801:Humans; D007223:Infant; D007225:Infant Food / Q000032:analysis* / Q000592:standards; D008461:Meat Products / Q000032:analysis* / Q000592:standards; D013211:Staphylococcus aureus; D014586:USSR
D004768:Enterotoxins; C026066:enterotoxin A, Staphylococcal; C031818:enterotoxin B, staphylococcal
Opredelenie stafilokokkovykh énterotoksinov tipov A i B v pishchevykh produktakh immunofermentnym metodom.