Inhibition of endothelin (ET)-1-and ET-2-induced vasoconstriction by anti-ET-1 monoclonal antibody.
We produced a monoclonal antibody to endothelin (ET)-1, tested cross-reactivities with the related peptides by enzyme immunoassay, and investigated the effects of the antibody on ET-1- or ET-2-induced vasoconstriction of rat isolated thoracic aorta. The antibody recognized ET-1, ET-2 and ET-3, and the immunoreactive site proved to be the N-terminal region but not the C-terminal region of ET-1. Moreover, at an approximate molar-equivalent concentration, the antibody absorbed ET-1 and ET-2, and significantly inhibited ET-1- and ET-2-induced vasoconstriction notwithstanding the presence of the endothelin receptor.
Tokyo Research Laboratories, Kowa Co. Ltd., Japan.
T
T
Koshi
T
T
Torii
K
K
Arai
T
T
Edano
M
M
Hirata
M
M
Ohkuchi
T
T
Okabe
D000818:Animals; D000911:Antibodies, Monoclonal; D016232:Endothelins / Q000037:antagonists & inhibitors* / Q000276:immunology; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D051381:Rats; D014661:Vasoconstriction / Q000187:drug effects*
D000911:Antibodies, Monoclonal; D016232:Endothelins