[The effects of insulin-like growth factor-I (IGF-I) and IGF-II on prolactin (PRL) release from human decidua and amniotic fluid circulation].
It has been reported that insulin-like growth factor (IGF) may play an important role in the feto-placental environment during pregnancy. The present study was undertaken to investigate the effect of IGF-I and IGF-II on prolactin (PRL) release from human decidual cells in vitro and in vivo. The human decidua in early pregnancy was obtained by D & C, and was enzymatically dispersed into a monocellular suspension. Monolayer cultures of these cells were exposed for 96 hours to either control media or medium supplemented with IGF-I and IGF-II. PRL levels in the media were measured by EIA. Five days after dispersion, the intracellular calcium concentration [( Ca]2+i) in cultured decidual cells was measured by the Fura-2 fluorescence method with a Spectrofluorometer. PRL and IGF-I levels in amniotic fluid of 2nd trimester and term pregnancy were measured by RIA for in vivo study. In an in vitro study, IGF-I increased PRL release from decidual cells significantly during a 96 hour culture. However, IGF-II did not enhance this PRL release. IGF-I stimulation had no effect on [Ca]2+i. In an in vivo study, amniotic fluid IGF-I levels in the 2nd trimester (139.8 +/- 28.1 ng/ml) were significantly higher than those in term pregnancy (46.4 +/- 14.2 ng/ml), and a significant positive correlation (r = 0.852, p less than 0.001) was observed between IGF-I and PRL levels in the amniotic fluid. The present in vivo and in vitro studies indicated that IGF-I plays an important role in PRL release from decidua into amniotic fluid.(ABSTRACT TRUNCATED AT 250 WORDS)
Kubota
T
T
Department of Obstetrics and Gynecology, Tokyo Medical and Dental University, Faculty of Medicine.
Kamada
S
S
Taguchi
M
M
Aso
T
T
D000653:Amniotic Fluid / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D003656:Decidua / Q000378:metabolism*; D005260:Female; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology / Q000502:physiology*; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology / Q000502:physiology*; D011247:Pregnancy / Q000378:metabolism; D011388:Prolactin / Q000378:metabolism*
D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D011388:Prolactin; D002118:Calcium