Stimulation of proliferation of bovine placental cells by products of activated mononuclear leukocytes.
Culture medium conditioned with concanavalin A-stimulated mononuclear leukocytes was tested for its ability to stimulate in vitro proliferation of bovine placental cells. The crude preparation of cytokines caused a dose-dependent increase in [3H]thymidine uptake into cells obtained by trypsinization of fresh bovine placentae and placental cell lines established from cellular outgrowths of long-term bovine placental cultures, but had no effect on growth of 3T3 fibroblasts. Growth of trypsinized placental cells was not enhanced by culture in the presence of interleukin-2, interferon-beta 2, interferon-gamma, or tumor necrosis factor-alpha. These results corroborate those of murine studies, suggesting a growth-promoting role for cytokines released into the maternal-fetal interface.
Low
B G
BG
Dairy Science Department, University of Florida, Gainesville 32611.
Hansen
P J
PJ
Drost
M
M
D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002823:Chorion / Q000166:cytology* / Q000187:drug effects; D003208:Concanavalin A; D016207:Cytokines / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D005260:Female; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000502:physiology*; D008213:Lymphocyte Activation; D010920:Placenta / Q000166:cytology* / Q000187:drug effects; D011247:Pregnancy; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D016207:Cytokines; D003208:Concanavalin A; D013936:Thymidine