The use of a urinary estrone conjugates assay for detection of optimal mating time in the cynomolgus macaque (Macaca fascicularis).
Forty-four female cynomolgus macaques (Macaca fascicularis) were examined to determine the optimum fertile period for mating. Daily urinary estrone conjugates (E1C) were measured, beginning on day 7 of the menstrual cycle, until a 1.5-gold E1C rise above the baseline was detected. The females were bred the next morning. Pregnancies were verified in all animals at day 18 postbreeding, and/or on day 25 postbreeding. Serum progesterone levels were used to correlate the relationship between ovulation and the E1C peak. Forty-four of the 57 cycles indicated a urinary E1C peak between days 10-15 of the menstrual cycle; this peak occurred on the day following the initial 1.5-fold to twofold rise in 90% of the cycles. A single 2-hr mating period the day before, the day of, or the day after the E1C peak resulted in conception in 17 of 44 (38.6%) animals.
Behboodi
E
E
Department of Obstetrics and Gynecology, University of California, Davis 95616.
Katz
D F
DF
Samuels
S J
SJ
Tell
L
L
Hendrickx
A G
AG
Lasley
B L
BL
D000818:Animals; D004970:Estrone / Q000652:urine*; D005260:Female; D005298:Fertility / Q000502:physiology*; D005306:Fertilization; D016231:Fluorescence Polarization Immunoassay; D008252:Macaca fascicularis / Q000097:blood / Q000502:physiology* / Q000652:urine; D008597:Menstrual Cycle / Q000502:physiology; D010061:Ovulation Detection / Q000662:veterinary; D011237:Predictive Value of Tests; D011247:Pregnancy; D011256:Pregnancy Outcome; D011258:Pregnancy Tests / Q000662:veterinary; D011374:Progesterone / Q000097:blood; D011863:Radioimmunoassay
D004970:Estrone; D011374:Progesterone