[Determining riboflavin level in urine (comparative analysis of the methods)].
Four methods of riboflavin content estimation in urine were studied: they are based on specific titration of vitamin B by riboflavin-binding protein (1), riboflavin decomposition by boiling in alkali (2), riboflavin fluorescence suppression by dithionate (3), and high-performance liquid chromatography. A conclusion has been made on the necessity of a home standard for riboflavin estimation by methods 1-3. The results obtained with the above methods correlated well, regression equations were derived describing correlation of these results. Methods 1 and 3 are most simple and highly specific and sensitive, therefore they could be recommended for wide use in large-scale clinical investigations.
Kodentsova
V M
VM
Alekseeva
I A
IA
Sokol'nikov
A A
AA
Bender
E D
ED
Iakushina
L M
LM
Spirichev
V B
VB
D000818:Animals; D001059:Apoproteins; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004527:Egg Proteins; D005260:Female; D006801:Humans; D012256:Riboflavin / Q000652:urine*; D013050:Spectrometry, Fluorescence / Q000379:methods
D001059:Apoproteins; D004527:Egg Proteins; D012256:Riboflavin
Opredelenie riboflavina v moche (sravnitel'nyĭ analiz metodov).