Responsiveness of the increase in C-fos mRNA levels depends on the inducer and the cell's past.
In this paper we show that in C3H10T1/2 mouse fibroblasts, the inducibility of c-fos mRNA by heat shock or serum addition is strongly dependent on the cell's past. Four hours after a heat shock, a time point where the induced c-fos mRNA has disappeared, c-fos mRNA could not be induced again by a second heat shock. Four hours after serum addition, by which c-fos was induced, a second serum addition also failed to induce c-fos mRNA again. When, however, serum was added 4 hours after heat shock or heat shock was given 4 hours after serum addition, levels of c-fos mRNA could be enhanced again. The induction by serum of c-fos mRNA levels in thermotolerant cells might be related to their increased stimulation of DNA synthesis as compared to control cells.
Tuijl
M J
MJ
Department of Molecular Cell Biology, University of Utrecht, The Netherlands.
Den Boon
J A
JA
Van Grunsven
W M
WM
Van Wijk
R
R
D000818:Animals; D001769:Blood; D002460:Cell Line; D003470:Culture Media; D005786:Gene Expression Regulation; D016762:Genes, fos; D006358:Hot Temperature; D007399:Interphase; D051379:Mice; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism
D003470:Culture Media; D012333:RNA, Messenger