Proton spectroscopic imaging: a tool for studying intracerebral tumor models in rat.
Water-suppressed 2D 1H spectroscopic imaging was used with surface coils to study in vivo the cerebral metabolism changes in rat brain induced by a glial tumor growing in situ. To achieve slice selection without a chemical-shift artifact, we exploited the depth pulse properties of a spin-echo sequence. In order to give a spectral response which is independent of the position, the water suppression was achieved by using a spin-locking excitation and a binomial refocusing pulse. Spectroscopic images were obtained with an in-plane resolution of 1.1 X 1.1 mm and a slice thickness of roughly 3 mm. The growing of the tumor induced dramatic modifications in the proton spectra, including a nearly complete loss of N-acetyl aspartate, an increase of the 1.3-ppm peak, an increase in choline, and a decrease in creatine. The results demonstrate the potential of spectroscopic imaging in the study of intracranial tumor models in rats.
Groupe de Résonance Magnétique en Biologie et Médecine, DRF/SPH, Centre d'Etudes Nucléaires, Grenoble, France.
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Bourgeois
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Remy
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Lefur
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Devoulon
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Benabid
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Decorps
D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology*; D001932:Brain Neoplasms / Q000175:diagnosis*; D005260:Female; D005910:Glioma / Q000175:diagnosis*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains