Transcriptional organization of the rfaGBIJ locus of Salmonella typhimurium.
The transcriptional organization of the rfaGBIJ gene cluster of Salmonella typhimurium was studied by using lacZ and cat transcriptional probes. The results indicated that the leftward end of the gene cluster (rfaG-rfaB-rfaI) is an operon that is transcribed from one or more promoters that lie upstream of rfaG. The results further indicated that the product of the rfaH (sfrB) gene acts as a positive regulator of transcription of the entire rfaGBIJ cluster. At least one site required for the RfaH-mediated transcriptional regulation lies within or very close to the upstream promoter.
Brazas
R
R
Department of Microbiology, University of Connecticut Health Center, Farmington 06032.
Davie
E
E
Farewell
A
A
Rothfield
L I
LI
D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D005810:Multigene Family; D011401:Promoter Regions, Genetic; D012486:Salmonella typhimurium / Q000235:genetics*; D014158:Transcription, Genetic