Singlet oxygen induced cerebral vasospasm: an experimental study in rats.
A new experimental model is described which can be used as an alternative to study the effects of subarachnoid hemorrhage (SAH) in rats. Vasospasm of the basilar artery is induced photochemically after transpalatal illumination of intracisternally injected rose bengal in two different rat strains. Singlet oxygen, generated in the subarachnoid space, elicits vasospasm which has been demonstrated angiographically at 90 min and 24 h after photosensitisation. Sprague Dawley rats responded better than Wistar rats. Dilution of rose bengal in water was more vasospastic than dilution of rose bengal in artificial CSF. Since the action of singlet oxygen is similar to that of free radicals, this experiment gives an argument for the hypothesis that free radicals play a leading role in the pathogenesis of cerebral vasospasm after subarachnoid hemorrhage.
University Hospital Antwerp, Department of Neurosurgery, Belgium.
J
J
Verlooy
J
J
Van Reempts
M
M
Haseldonckx
M
M
Borgers
P
P
Selosse
D000818:Animals; D001488:Basilar Artery / Q000000981:diagnostic imaging; D004195:Disease Models, Animal; D002546:Ischemic Attack, Transient / Q000139:chemically induced / Q000503:physiopathology*; D008297:Male; D010100:Oxygen; D010777:Photochemistry; D011859:Radiography; D051381:Rats; D011919:Rats, Inbred Strains; D026082:Singlet Oxygen; D013345:Subarachnoid Hemorrhage / Q000503:physiopathology*; D014711:Vertebral Artery / Q000000981:diagnostic imaging
D026082:Singlet Oxygen; D010100:Oxygen