Site-specific recombination promoted by a short DNA segment of plasmid R1 and by a homologous segment in the terminus region of the Escherichia coli chromosome.
A short DNA segment located in the kanamycin resistance region of plasmid R1 promotes site-specific recombination and plasmid maintenance. This segment has been reduced to 100 bp and subsequently to 44 bp without losing these properties. It can recombine with a similar segment located in the terminus region of the Escherichia coli chromosome. It is proposed that this recombination is responsible for the plasmid maintenance properties of the R1 segment. The chromosomal site has been isolated; it also shows site-specific recombination activity. Sequence homologies were also found with a phage site-specific integration locus in the chromosome of Xanthomonas campestris and with the plasmid ColE1 site-specific recombination locus. The recombinase required in all these systems is probably XerC, an E. coli enzyme acting on the cer site of plasmid ColE1 for the conversion of plasmid dimers to monomers. It is postulated that site-specific recombination in the terminus region of the chromosome intervenes in the partitioning of the two daughter chromosomes.
Clerget
M
M
Département de Biologie Moléculaire, Université de Genève, Switzerland.
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D002876:Chromosomes, Bacterial; D004261:DNA Replication; D004269:DNA, Bacterial / Q000502:physiology*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010316:Particle Size; D011815:R Factors / Q000235:genetics*; D011995:Recombination, Genetic; D016959:Xanthomonas campestris / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial