Crystallization and preliminary X-ray crystallographic analysis of verotoxin-1 B-subunit.
The B-subunit of verotoxin-1, which is believed to form a pentamer (monomer Mr = 7691), has been crystallized by vapor diffusion over a wide range of conditions. The best crystals, obtained with polyethylene glycol 8000 as the precipitant, belong to the orthorhombic space group P2(1)2(1)2(1), with cell dimensions a = 59.2 A, b = 102.7 A, c = 56.3 A. The cell dimensions are consistent with one B-subunit pentamer per asymmetric unit, and the crystals diffract to at least 2.0 A resolution. Data collected using synchrotron radiation at a wavelength of 2.070 A may allow the structure to be solved using the anomalous signal from three sulfur atoms in the monomer, combined with averaging over the non-crystallographic symmetry.
Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada.
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Boodhoo
R J
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Read
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Brunton
D001427:Bacterial Toxins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D022622:Shiga Toxin 1; D014961:X-Ray Diffraction
D001427:Bacterial Toxins; D022622:Shiga Toxin 1