Autonomous growth of human T-lymphotropic virus type I infected human lymphocytes treated with N-methyl-N'-nitro-N-nitrosoguanidine and ultraviolet rays.
Human T-cell cultures infected with human T-lymphotropic virus type I (HTLV-I) and interleukin-2 (IL-2)-dependent for their continuous growth were treated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and then maintained in the medium containing phorbol 12-myristate 13-acetate (TPA). Cells achieved independence from IL-2 but became TPA-dependent for continuous growth. Multiple ultraviolet (UV) irradiations of TPA-dependent cells resulted in their autonomous growth. G-band karyotype analysis revealed multiple chromosomal abnormalities that were seen in cells before and after MNNG treatment and UV irradiations, and those that were only seen in autonomously growing cells. Viral expression was found to be transiently enhanced in association with emergence of certain chromosomal changes. Exposure of HTLV-I infected cells to certain mutagens may promote the occurrence of the specific rearrangement of cellular genes responsible for regulation of cellular and viral replication and may lead these cells to neoplastic transformation.
Department of Pathology, Chiba Cancer Center Research Institute, Japan.
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K
Maruyama
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S
Mochizuki
K
K
Kawamura
C
C
Tamayama
D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002472:Cell Transformation, Viral / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D002478:Cells, Cultured; D002875:Chromosomes / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D003131:Combined Modality Therapy; D004279:DNA, Viral / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D005260:Female; D015490:HTLV-I Infections / Q000188:drug therapy* / Q000532:radiotherapy; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D006801:Humans; D007621:Karyotyping; D008214:Lymphocytes / Q000187:drug effects / Q000382:microbiology* / Q000528:radiation effects; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology* / Q000627:therapeutic use; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014466:Ultraviolet Rays
D004279:DNA, Viral; D008769:Methylnitronitrosoguanidine; D013755:Tetradecanoylphorbol Acetate