Allergen profiles of dog hair and dander, body fluids and tissues as defined by immunoblotting.
The sera from 25 patients with clinical type I allergy against dogs were investigated by means of immunoblotting, using extracts of dog hair/dander, skin, hair, saliva, salivary gland, serum and liver. 96% of the patients' sera showed IgE antibodies reactive with 19- and 23-kilodalton (kDa) proteins in the hair/dander extract. The 23-kDa IgE-binding protein was preferentially detected in the hair extract and saliva but not in skin, salivary gland, serum and liver extracts. The 19-kDa band was strongly expressed in skin, but not in hair, serum and liver. Inhibition experiments using the 23-kDa containing extract prepared from hair and the 19-kDa containing extract prepared from skin revealed that these two proteins are likely to be immunologically independent allergens.
Institute of Clinical Chemistry and Laboratory Diagnostics, University of Vienna, Austria.
S
S
Spitzauer
H
H
Rumpold
C
C
Ebner
C
C
Schweiger
R
R
Valenta
F
F
Gabl
J
J
Anrather
M
M
Breitenbach
O
O
Scheiner
D
D
Kraft
D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000032:analysis*; D000818:Animals; D001826:Body Fluids / Q000276:immunology*; D002648:Child; D004285:Dogs / Q000276:immunology*; D005260:Female; D006197:Hair / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D008222:Lymphokines / Q000032:analysis; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D029584:Prostatic Secretory Proteins; D012867:Skin / Q000276:immunology
D000485:Allergens; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors