A method for culturing rat oviduct gamma-aminobutyric acid cells.
A technique for the culture of rat oviduct gamma-aminobutyric acid (GABA) cells is described. The technique involves first explaining the fimbria and preampulla, which are the oviduct divisions with the highest density of GABA cells. The explanted tissue is cultured in a serum-free medium, to propagate the outgrowing cells. Under the experimental conditions we describe, the majority of the cells maintain GABA expression, as determined by immunostaining with a GABA antiserum.
Department of Environmental Health, University of Cincinnati Medical Center, OH 45267-0182.
I
I
Fernandez
E
E
Summers
M T
MT
Montero
L M
LM
Orensanz
G
G
Leikauf
D000818:Animals; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D003584:Cytological Techniques; D005187:Fallopian Tubes / Q000737:chemistry*; D005260:Female; D007150:Immunohistochemistry; D051381:Rats; D011919:Rats, Inbred Strains; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D016895:Culture Media, Serum-Free; D005680:gamma-Aminobutyric Acid