Both genes for EF-1 alpha in Candida albicans are translated.
In previous work, we showed that Candida albicans has two genes, TEF-1 and TEF-2, which encode identical polypeptides for the highly conserved, essential, protein synthesis factor EF-1 alpha (Breviario et al., 1988). This result prompted questions as to whether C. albicans preferentially uses one of the genes over the other and whether both genes are actually translated into protein. Gene-specific sequence differences in the untranslated portion of each gene made it possible to prepare gene-specific oligonucleotide hybridization probes. Results with the probes showed that the relative steady-state mRNA levels of the two genes were equivalent and that the mRNA for each gene was present in active translation complexes.
Department of Microbiology and Immunology, Texas College of Osteopathic Medicine, Fort Worth 76107.
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Irwin
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D015152:Blotting, Northern; D002176:Candida albicans / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D015342:DNA Probes; D005800:Genes, Fungal / Q000235:genetics; D005810:Multigene Family / Q000235:genetics; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000096:biosynthesis / Q000235:genetics*; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis
D015342:DNA Probes; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D012333:RNA, Messenger