Solcoseryl in prevention of stress-induced gastric lesions and healing of chronic ulcers.
Solcoseryl, a deproteinized extract of calf blood, protects the gastric mucosa against various topical irritants and enhances the healing of chronic gastric ulcerations but the mechanisms of these effects have been little studied. This study was designed to elucidate the active principle in Solcoseryl and to determine the role of prostaglandins (PG) and polyamines in the antiulcer properties of this agent. Using both, the radioimmunoassay and radioreceptor assay, EGF-like material was detected in Solcoseryl preparation. Solcoseryl given s.c. prevented the formation of stress-induced gastric lesions and this was accompanied by an increase in the generation of PGE2 in the gastric mucosa. Similar effects were obtained with EGF. Pretreatment with indomethacin, to suppress mucosal generation of prostaglandins (PG), greatly augmented stress-induced gastric ulcerations and antagonized the protection exerted by both Solcoseryl and EGF. Solcoseryl, like EGF, enhanced the healing of chronic gastro-duodenal ulcerations. This effect was abolished by the pretreatment with difluoromethylornithine, an inhibitor of ornithine decarboxylase, the key enzyme in the biosynthesis of polyamines. The healing effects of Solcoseryl and EGF was also reduced by prednisolone which decreased the angiogenesis in the granulation tissue in the ulcer area. These results indicate that Solcoseryl 1. contains EGF-like material, 2. displays the protective and ulcer healing effects similar to those of EGF and involving both PG and polyamines and 3. acts via similar mechanism as does EGF.
Konturek
S J
SJ
Institute of Physiology, University School of Medicine, Kraków, Poland.
Drozdowicz
D
D
Pytko-Polonczyk
J
J
Brzozowski
T
T
Bielański
W
W
D000183:Actihaemyl / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D000704:Analysis of Variance; D000818:Animals; D015232:Dinoprostone / Q000378:metabolism; D000518:Eflornithine / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000032:analysis* / Q000627:therapeutic use; D005753:Gastric Mucosa / Q000378:metabolism; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D011073:Polyamines / Q000494:pharmacology*; D011239:Prednisolone / Q000494:pharmacology; D011863:Radioimmunoassay; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D013276:Stomach Ulcer / Q000188:drug therapy* / Q000378:metabolism / Q000517:prevention & control; D013312:Stress, Physiological
D011073:Polyamines; D000183:Actihaemyl; D004815:Epidermal Growth Factor; D011239:Prednisolone; D015232:Dinoprostone; D007213:Indomethacin; D000518:Eflornithine