Comprehensive lectin histochemistry of normal and neoplastic human choroid plexus cells: alternation of lectin-binding patterns through neoplastic transformation.
Lectin histochemistry of the normal and neoplastic human choroid plexus cells [six choroid plexus papillomas (CPPs) and three choroid plexus carcinomas (CPCs)] was performed using eight representative lectins to study the development of sugar chain structures and also to determine whether lectins were useful for a histopathological diagnosis of choroid plexus neoplasms (CPNs). The normal choroid plexus cells reacted with Ricinus communis (RCA-I). Canavalia ensiformis (Con A), Limax flavus (LFA) and Triticum vulgaris (WGA), while Arachis hypoaea (PNA) stained them only after the removal of sialic acid. Human fetal choroid plexus cells at 8 weeks gestation already showed the same lectin-binding patterns as adult ones. All CPNs were stained by RCA-I and Con A in a similar manner as the normal choroid plexus cells. Although seven CPNs were positive for LFA, two CPCs were not stained by LFA, which bound to sialic acid. Two LFA-positive CPPs were stained by PNA before the removal of sialic acid. Moreover, unlike the normal choroid plexus cells, Ulex europaeus-, Glycine maximus- and Dolichos biflorus-binding sites often appeared, and WGA-binding sites of three CPNs remained even after sialic acid removal. In conclusion, the glycosialylation in normal choroid plexus cells was completed during the early embryonic stage. The lectin-binding patterns of CPNs were heterogenous in each case. The alternation of the glycosialylation and/or acquisition of binding sites for some lectins was sometimes observed through a neoplastic transformation.
Kaneko
Y
Y
Department of Neuropathology, Faculty of Medicine, Kyushu University, Japan.
Iwaki
T
T
Matsushima
T
T
Fukui
M
M
D000328:Adult; D002241:Carbohydrates / Q000032:analysis*; D002471:Cell Transformation, Neoplastic; D002831:Choroid Plexus / Q000166:cytology* / Q000196:embryology / Q000473:pathology; D016545:Choroid Plexus Neoplasms / Q000473:pathology*; D005260:Female; D005333:Fetus; D005865:Gestational Age; D006801:Humans; D037102:Lectins; D011247:Pregnancy; D012016:Reference Values
D002241:Carbohydrates; D037102:Lectins