Cytoskeletal changes induced by 2,5-hexanedione on developing human neurons in vitro.
Dissociated dorsal root ganglion cells from human fetuses were exposed to 2,5-hexanedione (2,5-HD) for 2 weeks. Morphological changes induced by 2,5-HD consisted in focal neurofilament (NF)-containing enlargements preferentially located in distal, preterminal regions of unmyelinated fibers. Tangles of NF were also observed in the perikarya of nerve cells. Morphometric analysis disclosed that the cross-sectional areas of the 2,5-HD treated axons were 30% smaller than those of control axons. This alteration was associated with reduction of number of NF per unit area. These findings demonstrate that 2,5-HD treatment induces a generalized disorganization of neuronal and axonal NF responsible for focal enlargements as well as atrophic changes of unmyelinated fibers.
Istituto di Neurologia, Università di Verona, Italy.
G
G
Moretto
S
S
Monaco
M G
MG
Passarin
M D
MD
Benedetti
N
N
Rizzuto
D001369:Axons / Q000187:drug effects; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects*; D005260:Female; D005727:Ganglia, Spinal / Q000187:drug effects; D006588:Hexanones / Q000633:toxicity*; D006801:Humans; D007382:Intermediate Filaments / Q000187:drug effects; D008870:Microtubules / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000648:ultrastructure; D011247:Pregnancy
D006588:Hexanones; C011269:2,5-hexanedione