Tissue levels of bone resorptive cytokines in periodontal disease.
The levels of 3 bone resorptive cytokines, interleukin 1 alpha (IL-1 alpha), IL-1 beta, and tumor necrosis factor alpha (TNF alpha), were assessed in tissues from sites of periodontal disease. As determined by ELISA of tissue extracts, IL-1 beta and TNF alpha were detected in all diseased sites, whereas IL-1 alpha was present in 8/22 sites, IL-1 beta was present in highest concentration (mean +/- SEM: 11,695 +/- 2,888 pg/ml; 672 pM), followed by TNF alpha (434 +/- 135 pg/ml; 26 pM), and IL-1 alpha (342 +/- 160 pg/ml; 20 pM). The levels of all 3 mediators were significantly lower in clinically healthy tissues. There was a highly significant correlation between levels of IL-1 beta and TNF alpha (rs = 0.61, P less than 0.001), suggesting coordinated expression of these 2 mediators. The numbers of cells containing each mediator was also determined by indirect immunofluorescence on frozen tissue sections. Consistent with findings from tissue extracts, IL-1 beta-containing cells were present in approximately 5-fold higher numbers than TNF alpha-containing cells, and 40-fold higher numbers than IL-1-alpha-containing cells. Taken together with previous findings, these results indicate that IL-1 beta is likely to be an important mediator in the pathogenesis of periodontal disease.
Stashenko
P
P
Forsyth Dental Center, Department of Immunology, Boston, MA.
Jandinski
J J
JJ
Fujiyoshi
P
P
Rynar
J
J
Socransky
S S
SS
D000328:Adult; D016301:Alveolar Bone Loss / Q000378:metabolism / Q000473:pathology; D000539:Alveolar Process / Q000737:chemistry / Q000473:pathology; D016207:Cytokines / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D004848:Epithelium / Q000737:chemistry / Q000473:pathology; D005455:Fluorescent Antibody Technique; D005881:Gingiva / Q000737:chemistry* / Q000473:pathology; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis*; D008875:Middle Aged; D010514:Periodontal Pocket / Q000378:metabolism / Q000473:pathology; D010518:Periodontitis / Q000378:metabolism* / Q000473:pathology; D010519:Periodontium / Q000737:chemistry / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D016207:Cytokines; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; C025940:bone resorption factor