Limitations due to unstirred layers in measuring channel response of excised membrane patch using rapid solution exchange methods.
The Ca(2+)-activated K+ channel was activated by a step increase in the intracellular Ca2+ concentration ([Ca2+]i) using the oil-gate concentration jump method in hippocampal neurons of rat. Limitations due to the unstirred volume between the excised inside-out membrane and the tip opening were examined.
Qin
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Department of Physiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Yoshida
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Noma
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D000818:Animals; D002118:Calcium / Q000378:metabolism; D004563:Electrochemistry; D006624:Hippocampus / Q000378:metabolism; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000502:physiology*; D009474:Neurons / Q000378:metabolism; D015221:Potassium Channels / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D015221:Potassium Channels; D002118:Calcium