Gastric mucosal repair in vitro.
We tested whether cultured gastric mucosal cells would be suitable to study the two major steps of repair: restitution and proliferation. Preparations of freshly isolated epithelial cells of guinea pig gastric mucosa were used for the studies. The cells attached to Petri dishes within 10 h and formed monolayers after 48-72 h. Electron microscopy showed that each cell type was able to form lamellipodia (i.e., cell protrusions) during restitution in vivo. When monolayers were wounded with a razor blade, most cells at the edge of damage died within a few minutes, but some recovered from injury. Later, intact cells migrated from the edge into the denuded zone and restored the monolayer within 24-48 h. An increased number of cells near the edge started to synthesize DNA. In conclusion, this model allows one to study in vitro both aspects of mucosal repair.
Department of Pharmacology, Medical School Hannover, Federal Republic of Germany.
J
J
Giebel
K U
KU
Thiedemann
M
M
Schwenk
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004847:Epithelial Cells; D004848:Epithelium / Q000503:physiopathology; D005753:Gastric Mucosa / Q000503:physiopathology*; D006168:Guinea Pigs; D008297:Male; D013936:Thymidine / Q000378:metabolism; D014945:Wound Healing
D004247:DNA; D013936:Thymidine