Stimulation of anti-RNP antibody binding to cultured keratinocytes by estradiol.
Antibodies to extractable nuclear antigens (ENA) are frequently demonstrated in the serum of patients with cutaneous lupus erythematosus. To gain a better understanding of the influence of sex hormones on cutaneous lupus, we investigated the in vitro binding of anti-ENA antibodies to cultured human keratinocytes. Estradiol-beta augmented the binding of anti-RNP, anti-SS-A/Ro, and anti-SS-B/La antibodies to cultured cells, but produced no enhancement of the binding of anti-Sm antibodies. In addition, we examined the effects of 16-alpha-hydroxyestrone, a highly estrogenic metabolite, on the binding of anti-ENA antibodies to cultured cells. This agent did not produce any augmentation of binding. Based on these experiments, we discuss the influence of estradiol on cutaneous lupus.
Furukawa
F
F
Department of Dermatology, Faculty of Medicine, Kyoto University, Japan.
Imamura
S
S
Norris
D A
DA
D000974:Antibodies, Antinuclear / Q000378:metabolism; D001323:Autoantibodies / Q000378:metabolism*; D001324:Autoantigens / Q000276:immunology* / Q000378:metabolism; D001666:Binding Sites, Antibody; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D006894:Hydroxyestrones / Q000378:metabolism; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D055517:snRNP Core Proteins
D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D001324:Autoantigens; D006894:Hydroxyestrones; C035356:SS-A antibodies; C035357:SS-B antibodies; D055517:snRNP Core Proteins; C026702:16-hydroxyestrone; D004958:Estradiol