The Drosophila roughened mutation: activation of a rap homolog disrupts eye development and interferes with cell determination.
Roughened is a dominant mutation of D. melanogaster that disrupts eye development. The majority of the ommatidia in the adult eye lack a single photoreceptor cell, which is most commonly the R7 cell. The Roughened mutation disrupts the early stages of photoreceptor cell determination. Roughened is a dominant gain-of-function mutation that results from a single amino acid change (Phe157 to Leu) in the Drosophila Rap1 protein. Loss of function Rap1 mutations are lethal. Drosophila Rap1 protein is 88% identical to human rap1A/K-rev1 protein, a putative antagonist of ras action.
Hariharan
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IK
Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Carthew
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RW
Rubin
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GM
D000595:Amino Acid Sequence; D000818:Animals; D004331:Drosophila melanogaster / Q000196:embryology* / Q000235:genetics; D005123:Eye / Q000196:embryology*; D019204:GTP-Binding Proteins / Q000502:physiology*; D005799:Genes, Dominant; D008969:Molecular Sequence Data; D009154:Mutation; D016415:Sequence Alignment; D020668:rap GTP-Binding Proteins
D019204:GTP-Binding Proteins; D020668:rap GTP-Binding Proteins