Sphingolipids of a cestode Metroliasthes coturnix.
Sphingolipids of Metroliasthes coturnix were studied. The cestode contained no detectable amounts of sphingomyelin. The major glycosphingolipids found were monogalactosylceramide, galactosyl-alpha-1-4-galactosylceramide, galactosyl-beta-1-6-galactosyl-beta-1-6-galactosylceramide and galactosyl-beta-1-6-galactosyl-beta-1-6-galactosyl-beta-1-6-galactosylce ramide. Their ceramides were mostly composed of C18-20 sphinganine or 4-D-hydroxysphinganine, which is N-acylated by extraordinarily long normal and 2-hydroxy acids such as C26 acid (range C16 to C30) except tetragalactosylceramide. The structure of glycosphingolipids was confirmed after their chromatographic separation into each molecular species using a novel analytical device, fast atom bombardment-mass spectrometry linked with reversed-phase high-performance liquid chromatography.
Department of Biology, Keio University, Medical School, Yokohama, Japan.
K
K
Nishimura
A
A
Suzuki
H
H
Kino
D000215:Acylation; D000818:Animals; D002240:Carbohydrate Sequence; D002518:Ceramides / Q000032:analysis / Q000737:chemistry; D002589:Cestoda / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D006028:Glycosphingolipids / Q000032:analysis / Q000737:chemistry; D008969:Molecular Sequence Data; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013107:Sphingolipids / Q000032:analysis* / Q000737:chemistry; D013110:Sphingosine / Q000031:analogs & derivatives / Q000032:analysis
D002518:Ceramides; D006028:Glycosphingolipids; D013107:Sphingolipids; D013110:Sphingosine; C005682:safingol