No association between susceptibility to multiple sclerosis and HLA-DPB1 alleles in the French Canadian population.
HLA-DPB1 typing was performed using polymerase chain reaction DNA amplification and sequence-specific oligonucleotide probing (PCR-SSOP) which permitted identification of 17 distinct DPB alleles using 15 oligonucleotide probes. The accuracy of this approach was confirmed in an initial study of 26 human B-lymphoblastoid cell lines which demonstrated close agreement between PCR-SSOP and PLT assigned types. A cohort of 47 adult French Canadians was then studied to provide an estimate of DPB1 allelic frequencies in an ethnically homogeneous population. DPB1*0401 was the most frequent phenotype (61.5%) and only DPB1*0101, 0301 and 0402 were also present at frequencies greater than 10%. HLA-DPw4 has been reported to be associated with multiple sclerosis (MS) but our PCR-SSOP analysis of 52 French Canadian MS patients showed no association with either the DPB1*0401 or DPB1*0402 splits of DPw4 or with any other DPB1 allele.
Wessex Histocompatibility Group, Southampton General Hospital, U.K.
W M
WM
Howell
D A
DA
Sage
P R
PR
Evans
J L
JL
Smith
G S
GS
Francis
D G
DG
Haegert
D000483:Alleles; D001483:Base Sequence; D002170:Canada; D015331:Cohort Studies; D004198:Disease Susceptibility / Q000208:ethnology / Q000276:immunology; D005006:Ethnicity; D020022:Genetic Predisposition to Disease; D006682:HLA-DP Antigens / Q000235:genetics*; D059868:HLA-DP beta-Chains; D006801:Humans; D008969:Molecular Sequence Data; D009103:Multiple Sclerosis / Q000208:ethnology / Q000235:genetics / Q000276:immunology*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction
D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen; D015345:Oligonucleotide Probes