Monoclonal antibodies monospecific to single-chain urokinase-type plasminogen activator (scu-PA).
Until now, single-chain urokinase-type plasminogen activator (scu-PA) could not be distinguished from urokinase (UK) by immunological methods. Therefore, in our study monoclonal antibodies (mAbs) specific for scu-PA were raised in mice. These mAbs proved to discriminate between scu-PA, UK and plasmin-activated scu-PA. The clones producing these mAbs could be cultivated in a serum-free medium. Furthermore, very pure preparations were obtained by a one-step purification procedure on protein-A-sepharose. The mAbs described provide a future potential for immuno-affinity chromatography and for the specific determination of scu-PA.
Institut für Klinische Immunologie und Rheumatologie der Universitat Erlangen-Nürnberg, FRG.
J G
JG
Scheuerlein
I
I
Kalies
W P
WP
Gluth
J R
JR
Kalden
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D003429:Cross Reactions; D005260:Female; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D014568:Urokinase-Type Plasminogen Activator / Q000276:immunology* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D014568:Urokinase-Type Plasminogen Activator