[A novel approach to the detection of Neisseria gonorrhoeae employing DNA probe method].
The present study was undertaken to establish a novel detection method for Neisseria gonorrhoeae (N. gonorrhoeae). DNA probe obtained from Gen-probe Inc. was employed and clinically isolated strains (18 species, 81 strains) were applied to verify the quality and quantity of the method. The present data revealed that the method is specific to detect N. gonorrhoeae (30/30): neither gram positive nor negative oraganisms were detectable by the method (0/51). It has been shown, further more, that there exists a significant correlation between the number of the bacteria and the relative light units (r = 0.96). The threshold of cell numbers, on the other hand, was found to be 5 X 10(3) cfu/tube. The detection sensitivity for N. gonorrhoeae was not influenced by the presence of other bacteria, and not decreased by using heat-treated or drug-treated N. gonorrhoeae organisms. The present study also provides some experimental evidences that the method can also be applicable for the detection of bacteriolytic action of drugs.
Harada
Y
Y
Department of Microbiology, Toho University School of Medicine.
Miyazaki
S
S
Kaneko
Y
Y
Goto
S
S
D001431:Bacteriological Techniques; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D015342:DNA Probes; D004269:DNA, Bacterial