Quantitative platelet imaging and comparison of four mathematical models for the evaluation of platelet mean life span in patients with chronic immune thrombocytopenia.
METHODS
Twenty-five thrombocytopenic patients underwent quantitative in vivo platelet kinetic studies using a scintillation camera and a computer-assisted imaging system. They fulfilled the criteria for chronic immune thrombocytopenia, and the object of the study was to evaluate platelet sequestration and destruction in the spleen. In this sense, the differences in the distribution of the radiolabelled platelets in the spleen and the liver, as well as the differences in the platelet mean life span (MLS) as computed by different methods were assessed. Serial images of the spleen pool, liver and heart were taken after reinjection of In-111-oxine labelled autologous platelets.


CONCLUSIONS
The spleen/liver ratio proved to be a more reliable index in indicating the splenectomy outcome that the Z index (see text). Platelet survival curves were analyzed using four mathematical models, and it was found that MLS estimation is model dependent. Thus, it seem advisable to apply at least two different methods when analyzing experimental data.
Amuasi
J H
JH
Divisione di Ematologia, USL 10/D, Firenze, Italy.
Ferri
P
P
Grossi
A
A
Vannucchi
L
L
Vannucchi
A M
AM
Ferrini
P R
PR
D001327:Autoimmune Diseases / Q000097:blood / Q000000981:diagnostic imaging*; D001792:Blood Platelets / Q000000981:diagnostic imaging* / Q000473:pathology; D016922:Cellular Senescence; D002908:Chronic Disease; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007205:Indium Radioisotopes; D007700:Kinetics; D008099:Liver / Q000000981:diagnostic imaging; D008954:Models, Biological; D009942:Organometallic Compounds; D015125:Oxyquinoline / Q000031:analogs & derivatives*; D010976:Platelet Count / Q000379:methods*; D011877:Radionuclide Imaging; D013154:Spleen / Q000000981:diagnostic imaging; D013921:Thrombocytopenia / Q000097:blood / Q000000981:diagnostic imaging*
D007205:Indium Radioisotopes; D009942:Organometallic Compounds; C032950:indium oxine; D015125:Oxyquinoline