Purification and interaction properties of the human RNA polymerase B(II) general transcription factor BTF2.
A general transcription factor (BTF2) has been purified from HeLa whole cell extract and shown to be absolutely required for the formation of a functional initiation complex. We also demonstrate that this factor binds in solution to RNA polymerase B(II) and to the other general transcription factors BTF1(TFIID), BTF3, and BTF4. These results strongly suggest the existence of multiple interactions between the various components of the multiprotein initiation complex.
Gerard
M
M
Laboratoire de Génétique Moléculaire des Eucaryotes, Institut National de la Santé et de la Recherche Médicale, Faculté de Médecine.
Fischer
L
L
Moncollin
V
V
Chipoulet
J M
JM
Chambon
P
P
Egly
J M
JM
D006367:HeLa Cells / Q000737:chemistry*; D006801:Humans; D046911:Macromolecular Substances; D008970:Molecular Weight; D046912:Multiprotein Complexes; D012319:RNA Polymerase II / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D014158:Transcription, Genetic
D046911:Macromolecular Substances; D046912:Multiprotein Complexes; D014157:Transcription Factors; D012319:RNA Polymerase II