Mutagenicity of various chemicals including nickel and cobalt compounds in cultured mouse FM3A cells.
Employing a suspension culture of FM3A cells, we examined the cytotoxic and mutagenic effects of various chemical compounds. Mutagenicity of various types of mutagens (MNNG, ENNG, sterigmatocystin, mitomycin C, Trp-P-1, and X-rays) was sensitively detected by this assay. Mutagenicity of Trp-P-2 was detected in the presence of an activating enzyme system. Nickel(II) and cobalt(II) compounds (NiCl2, Ni(CH3COO)2, nickel complex [(C2H5)4N]2 [NiCl4], CoCl2, and a cobalt complex [(C2H5)4N]2-[CoCl4]) were cytotoxic to FM3A cells at concentrations of over 1 X 10(-4) M, and produced 2-6-fold increases of the control in the average number of 6-thioguanine-resistant (6TGr) colonies over a very narrow concentration range of 2-4 X 10(-4) M. Comparison of the mutagenicity of various chemical compounds suggested that some of the nickel(II) and cobalt(II) compounds were very weak mutagens.
Kihara Institute for Biological Research, Yokohama City University, Japan.
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Morita
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Umeda
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Ogawa
D000085:Acetates / Q000633:toxicity; D019342:Acetic Acid; D000818:Animals; D002243:Carbolines / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003035:Cobalt / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D066298:In Vitro Techniques; D051379:Mice; D008809:Mice, Inbred C3H; D016685:Mitomycin / Q000633:toxicity; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009532:Nickel / Q000633:toxicity*; D013241:Sterigmatocystin / Q000633:toxicity; D014965:X-Rays / Q000009:adverse effects
D000085:Acetates; D002243:Carbolines; D009153:Mutagens; D013241:Sterigmatocystin; D003035:Cobalt; D016685:Mitomycin; C022838:nickel chloride; D009532:Nickel; C018021:cobaltous chloride; C019132:3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole; D019342:Acetic Acid