[Diagnosis of Chlamydia trachomatis--culture or enzyme immunoassay? Norwegian multicenter study].
Two EIA-tests (Chlamydiazyme, Abbott and Ideia, Celltech) were compared with culture for diagnosis of chlamydial infection in 1,185 patients from four different regions in Norway. The EIA-tests showed only minor differences in prevalences between the different regions (Chlamydiazyme: 4.1% to 5.4%; Ideia: 5.1% to 5.7%), but the agreement between the EIA-results was not satisfactory. 4.6 per cent of the specimens from Trondheim were culture-positive, but only 2.0% of the specimens from the other regions were culture-positive. Compared with the results from cultures from Trondheim (n = 349), the sensitivity, specificity, positive and negative predictive values for the EIA-tests were: Chlamydiazyme: 81.3%, 98.2%, 68.4% and 99.1% (81.3%, 96.4%, 52.0% and 99.1% without blocking test); Ideia: 93.8%, 98.5%, 75.0% and 99.7%. Culture should not be used as a routine method for detection of chlamydia in specimens sent by post without cooling.
Avdeling for mikrobiologi, Regionsykehuset i Trondheim.
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SA
Nordbø
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A
Mehl
I S
IS
Vik
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E
Ragnhildstveit
D001431:Bacteriological Techniques; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000201:enzymology / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male
Diagnostikk av Chlamydia trachomatis--dyrking eller enzymimmunoassay? En norsk multisenterundersøkelse.