Methamphetamine-induced expression of interleukin-1 beta mRNA in the rat hypothalamus.
The effect of methamphetamine on the expression of interleukin-1 beta (IL-1 beta) mRNA in the rat brain was investigated with Northern blot analysis. Methamphetamine (2-15 mg/kg, i.p.) caused a marked induction of IL-1 beta mRNA in the hypothalamus among the 8 brain regions examined, in a dose-dependent manner. The level of IL-1 beta mRNA reached a maximum at 1 h and rapidly declined within 2 h after the injection of 15 mg/kg. These results provide the first evidence that methamphetamine induces the expression of IL-1 beta mRNA in the hypothalamus, which may be partly involved in the production of central actions of this drug.
Yamaguchi
T
T
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Kuraishi
Y
Y
Minami
M
M
Nakai
S
S
Hirai
Y
Y
Satoh
M
M
D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D007031:Hypothalamus / Q000378:metabolism*; D007375:Interleukin-1 / Q000235:genetics*; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology*; D011433:Propranolol / Q000494:pharmacology; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D007375:Interleukin-1; D012333:RNA, Messenger; D008694:Methamphetamine; D011433:Propranolol