Cloning, sequence analysis and transcriptional expression of a ras gene of the edible basidiomycete Lentinus edodes.
In the edible basidiomycete, Lentinus edodes, the presence of a high level of intracellular cyclic AMP (cAMP) is closely related to the onset of fruiting and/or primordium formation. Since a close relationship between intracellular cAMP levels and expression of ras genes was reported for organisms such as Saccharomyces cerevisiae and Dictyostelium discoideum, we have cloned and sequences a ras gene homologue from L. edodes (Le.), and analyzed its expression during development of the fungus. This gene, named Le.ras, has a coding capacity of 217 amino acids (aa) interrupted by six small introns. The deduced Le.Ras protein exhibited the highest homology to the Schizosaccharomyces pombe RAS protein (219 aa): 86% homology in the N-terminal 80-aa sequence and 74% homology in the next 80 aa. The Le.ras gene was transcribed at similar levels during mycelial development in fruiting-body formation, suggesting no direct correlation of Le.ras expression with intracellular cAMP levels in this organism.
Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.
K
K
Hori
S
S
Kajiwara
T
T
Saito
H
H
Miyazawa
Y
Y
Katayose
K
K
Shishido
D000107:Acetylation; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D015342:DNA Probes / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D011124:Polyporaceae / Q000235:genetics* / Q000254:growth & development; D018631:ras Proteins
D015342:DNA Probes; D005656:Fungal Proteins; C489016:ras protein, Dictyostelium; D018631:ras Proteins