Utilization of methoxylated aromatic compounds by the acetogen Clostridium thermoaceticum: expression and specificity of the co-dependent O-demethylating activity.
The aromatic CO-dependent O-demethylating activity of Clostridium thermoaceticum was evaluated. Secondary aromatic substituent groups (-OH, -CO2H, -CH2OH, and -OCH3) were critical to O demethylation. O-demethylating activities and specificities were similar from cells grown at the expense of different methoxylated aromatic compounds; all O-methyl-grown cells catalyzed the same sequential O demethylation of multi-methoxylated compounds, suggesting that a broad specificity O demethylase was involved in O demethylation. In cell-fractionation studies, CO-dependent O demethylation was catalyzed by membrane-associated components.
Department of Biology, University of Mississippi, University 38677.
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Daniel
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Keith
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Yang
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Lin
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Drake
D000840:Anisoles / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology; D003013:Clostridium / Q000187:drug effects* / Q000201:enzymology; D062385:Hydroxybenzoates / Q000494:pharmacology; D008745:Methylation; D010090:Oxidoreductases, O-Demethylating / Q000378:metabolism*; D013379:Substrate Specificity
D000840:Anisoles; D062385:Hydroxybenzoates; D010090:Oxidoreductases, O-Demethylating