Very early detection of changes associated with cellular activation using a modified flow cytometer.
A sample station modification previously described has been redesigned to provide greater flexibility and enhanced performance. The improved modification provides mixing and temperature regulation in a compact unit that mounts close to the nozzle holder for reduced transit times, allowing for addition of mediators to a sample in place on the flow cytometer, with observation of results in approximately 1 s. An electronic circuit activated at the time of injection generates full-scale pulses in the forward scatter channel. This provides the data with a time stamp for direct correlation of injection and cellular response. A detailed description of the modification, performance verification data, and practical applications in the measurements associated with cellular activation are presented.
Kelley
K A
KA
Institute for Arthritis and Autoimmunity, Miles Research Center, West Haven, Connecticut 06516.
D002118:Calcium / Q000378:metabolism; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials / Q000502:physiology; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D010084:Oxidation-Reduction
D002118:Calcium