[Studies on plasma immunoreactive human brain natriuretic peptide (hBNP) levels in patients with congestive heart failure and present forms of hBNP in human cardiac ventricle].
By using a specific radioimmunoassay (RIA) for human brain natriuretic peptide (hBNP), we measured immunoreactive hBNP (ir-hBNP) in plasma from patients with congestive heart failure (CHF). There appeared to be relationship between the enhanced ir-hBNP secretory activity and the severity of the failing heart as well as that of immunoreactive human atrial natriuretic peptide (ir-hANP). However, the secretion of ir-hBNP was augmented much more than that of ir-hANP in sever CHF patients. Gel permeation chromatography coupled with the RIA revealed that ir-hBNP in human ventricle is composed with gamma-hBNP and hBNP (1-32) as well as that of human atrium. However, we found differences of the gamma-hBNP/hBNP (1-32) ratio in atrial and ventricular tissues. These findings suggest that the hBNP secretion mechanism differ in the two areas of human heart.
Togashi
K
K
Department of Molecular Biology, Kitasato University School of Medicine, Sagamihara.
Kameya
T
T
Ando
K
K
Marumo
F
F
Kawakami
M
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006325:Heart Atria / Q000378:metabolism; D006333:Heart Failure / Q000097:blood*; D006352:Heart Ventricles / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism*; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000378:metabolism*; D011863:Radioimmunoassay
D009419:Nerve Tissue Proteins; D020097:Natriuretic Peptide, Brain