High-performance liquid chromatographic investigation of product formation in the horseradish peroxidase-enhanced chemiluminescence of luminol with different enhancers.
A reversed-phase high-performance liquid chromatographic method for the investigation of the chemiluminescence-producing oxidation of luminol by the enzyme horseradish peroxidase is presented. Both the kinetics and the mechanism of product formation can be monitored. Special attention is paid to the mechanism of enhancement of the chemiluminescence by phenolic compounds, such as p-iodophenol, p-hydroxycinnamic acid and 6-hydroxybenzothiazole. The function of the enhancers was elucidated partially by the observation of a higher degradation rate of luminol. In addition, it was concluded that the mechanism of enhancement is probably different for the various enhancers, based on the product formation.
Laboratory for Toxicology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
E H
EH
Jansen
R H
RH
van den Berg
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006868:Hydrolysis; D008163:Luminescent Measurements; D008165:Luminol / Q000378:metabolism*; D010636:Phenols
D010636:Phenols; D008165:Luminol; D006735:Horseradish Peroxidase