Linkage mapping and fluorescence in situ hybridization of TCTE1 on human chromosome 6p: analysis of dinucleotide polymorphisms on native gels.
Highly informative dinucleotide repeat polymorphisms were identified at the T-complex-associated-testes-expressed-1 (TCTE1) locus on human chromosome 6p. Electrophoresis of single-stranded DNA on native gels facilitated the analysis of the dinucleotide polymorphisms. Linkage mapping positions this marker midway between the centromere and HLA with recombination fractions as follows: D6Z1-0.21-TCTE1-0.24-HLA. Two-color fluorescence in situ hybridization places TCTE1 proximal to CRIL171 (D6S19). Together, linkage and in situ hybridization indicate that the order of the loci is D6Z1-D6S4-D6S90-TCTE1-D6S19-D6S29-HL A-telomere. A sequence tagged site (STS) was established, and three yeast artificial chromosome (YAC) clones were identified for the TCTE1 locus.
Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030.
T J
TJ
Kwiatkowski
A L
AL
Beaudet
B J
BJ
Trask
H Y
HY
Zoghbi
D000483:Alleles; D001483:Base Sequence; D044383:Black People; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015226:Dinucleoside Phosphates / Q000032:analysis*; D005260:Female; D005787:Gene Frequency; D008040:Genetic Linkage; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014481:United States; D044465:White People; D001741:Black or African American
D015226:Dinucleoside Phosphates; D009838:Oligodeoxyribonucleotides; D004247:DNA