An in vitro model to study diabetic neuropathy.
Adult dorsal root ganglion (DRG) neurons from diabetic and normal C57BL mice were cultured and compared for survival and neurite extension. Neurons from diabetic mice have improved their survival and neurite extension when the insulin concentration was increased but it was never as good as that of controls. The increase of glucose concentration in serum-free media to ten times higher than normal improved both survival and neurite extension of neurons from diabetic animals.
Sotelo
J R
JR
Biophysics Department, Instituto de Investigaciones Biologicas Clemente Estable, Montevideo, Uruguay.
Horie
H
H
Ito
S
S
Benech
C
C
Sango
K
K
Takenaka
T
T
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000473:pathology; D003929:Diabetic Neuropathies / Q000473:pathology*; D005727:Ganglia, Spinal / Q000187:drug effects / Q000473:pathology; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D016501:Neurites / Q000473:pathology; D009474:Neurons / Q000473:pathology*
D007328:Insulin; D005947:Glucose