Benextramine irreversibly inhibits [125I]neuropeptide Y affinity labeling of the Y2 binding protein in bovine hippocampus.
Affinity labeling of iodinated neuropeptide Y (NPY) to bovine hippocampal binding proteins revealed that benextramine inhibited specific NPY labeling of the 50 kDa NPY binding protein (Y2 binding protein) in a dose-dependent manner (IC50 = 33 microM). Hippocampal membranes, which were pretreated with benextramine and washed, exhibited decreased [125I]NPY labeling of binding proteins in a similar dose-dependent manner. These findings demonstrate that benextramine irreversibly blocks specific NPY binding to the 50 kDa NPY Y2 binding protein.
Department of Pharmacology, University of Nebraska Medical Center, Omaha 68198-6260.
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D000345:Affinity Labels; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002417:Cattle; D003432:Cross-Linking Reagents / Q000494:pharmacology; D003538:Cystamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006624:Hippocampus / Q000378:metabolism*; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008566:Membranes / Q000378:metabolism; D009478:Neuropeptide Y / Q000378:metabolism*; D013388:Succinimides / Q000494:pharmacology
D000345:Affinity Labels; D002352:Carrier Proteins; D003432:Cross-Linking Reagents; D007457:Iodine Radioisotopes; D009478:Neuropeptide Y; D013388:Succinimides; C029998:benextramine; D003538:Cystamine; C019358:disuccinimidyl suberate