Light chain heterogeneity in the amphibian Xenopus.
Three subpopulations of light chains in Xenopus can be distinguished by monoclonal antibodies as well as by electrophoretic mobility on SDS-PAGE, peptide map and cell surface distribution. Analysis of these proteins from LPS-stimulated lymphocytes culture supernatants by two-dimensional gel electrophoresis showed a heterogeneity comparable to that observed for mouse kappa light chains. However, evidence from the selective expression of light chain subpopulations, as well as highly restricted light chain representation in anti-DNP antibodies, supports earlier findings that an antibody response in Xenopus is greatly limited in heterogeneity.
Basel Institute for Immunology, Switzerland.
E
E
Hsu
I
I
Lefkovits
M
M
Flajnik
L
L
Du Pasquier
D000818:Animals; D000911:Antibodies, Monoclonal; D000916:Antibody Diversity; D000918:Antibody Specificity; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007422:Intestines / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D014982:Xenopus laevis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007147:Immunoglobulin Light Chains