Oocyte and zygote zona pellucida lectin binding in BALB/cBy and C57BL/6By mice and their F1 hybrids.
Zona pellucida intact oocytes and zygotes from two inbred strains of mice (BALB/cByEss and C57BL/6ByEss) and their F1 hybrids were reacted with a lectin panel (ConA, WGA, sWGA, PNA, UEA I, LTA, BSB4, DBA, PHA-P, LPA, and LFA). No major differences were observed between groups of mice for the majority of the lectin binding patterns. However, oocytes from BALB/cByEss and the F1 (C57BL/6ByxBALB/cBy) gave identical binding patterns for PNA. Following fertilization BALB/cByEss and the F1 (C57BL/6ByxBALB/cBy) bound UEAI and LTA more strongly, but the other two groups of mice demonstrated identical weaker binding of UEAI and LTA. These results indicate the possible influence of the paternal genotype on zona pellucida formation.
Department of Biology, University of Essex, Colchester, England, United Kingdom.
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D000818:Animals; D002478:Cells, Cultured; D003433:Crosses, Genetic; D005260:Female; D006001:Glycoconjugates / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000378:metabolism*; D013045:Species Specificity; D015044:Zona Pellucida / Q000378:metabolism*; D015053:Zygote / Q000378:metabolism*
D006001:Glycoconjugates; D037102:Lectins