Mobility and electrophoretic mobility of long linear polymer molecules in gels.
Theoretical expressions for mobility and specific (per unit charge) electrophoretic mobility of long linear molecules in gels are derived in one-dimensional approximations. The influence of the gel is described by a voluminal friction coefficient which is shown to be a reciprocal of specific hydrodynamic permeability of the gel. The nonmonotonous profile of liquid flow velocity in the vicinity of the molecular surface is obtained. Near the surface of the molecule, the liquid flows in the direction of electrophoretic movement. At a distance from the molecule, the direction of liquid flow is in the opposite direction. The distance of the point with zero velocity from the surface of the molecule decreases with increase of gel density. The specific electrophoretic mobility of a straight molecule in the gel is equal to half the harmonic average of the mobility of the molecule in the gel and the specific electrophoretic mobility of the same molecule in gel-free electrolyte solution. The theoretical expression for friction coefficient is given. The results will be used for theoretical analysis of PFGE experiments.
Pastushenko VPh
A. N. Frumkin Institute of Electrochemistry, USSR Academy of Sciences, Moscow.
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D011108:Polymers / Q000737:chemistry*
D011108:Polymers