Contribution of patient history to the glutathione S-transferase activity of human lung, breast and colon tissue.
Overexpression of the glutathione S-transferases (GSTs) and their involvement in the detoxification of anticancer agents has prompted numerous investigations of the enzyme activity of human tumor tissue. This study represents an in-depth evaluation of the contribution of patient history and pathological status to the GST activity of various human tissues. GST activity was elevated significantly in tumors of the lung, breast and colon as compared to unmatched and matched normal tissue from the same organ. The GST activity of primary breast tumors varied significantly with the stage of the tumor. Breast tumors previously treated with both radiation and chemotherapy had significantly lower levels of GST activity than untreated tumors. Neither progesterone nor estrogen receptor content was associated with the GST activity in primary breast tumors. Colon metastases possessed higher levels of GST activity than primary colon tumors but enzyme activity was independent of the Duke's classification of the tumor. Only tumors of the left colon had levels of GST activity that were higher than those of adjacent normal mucosa. No relationship was evident between either age or sex and the GST activity of any of the tissues examined. GST activity levels may reflect the site-specific ability of tissues to provide cellular protection against xenobiotics.
Department of Pharmacology, Fox Chase Cancer Center, Philadelphia, PA 19111.
M L
ML
Clapper
S J
SJ
Hoffman
N
N
Carp
P
P
Watts
L M
LM
Seestaller
J L
JL
Weese
K D
KD
Tew
D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism; D001940:Breast / Q000201:enzymology*; D001943:Breast Neoplasms / Q000201:enzymology; D003106:Colon / Q000201:enzymology*; D003110:Colonic Neoplasms / Q000201:enzymology / Q000556:secondary; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000201:enzymology*; D008175:Lung Neoplasms / Q000201:enzymology; D008297:Male; D009376:Neoplasms, Hormone-Dependent / Q000201:enzymology / Q000648:ultrastructure; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D012307:Risk Factors; D012907:Smoking / Q000378:metabolism
D014408:Biomarkers, Tumor; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D005982:Glutathione Transferase