A practicable two-dimensional electrophoresis of urinary proteins as a useful tool in medical diagnosis.
Practical experience with a rapid two-dimensional electrophoresis technique for routine analysis of urinary proteins is discussed. The method consists of cellulose acetate electrophoresis in combination with sodium dodecyl sulfate (SDS) electrophoresis, performed together with Coomassie Blue gel staining on "PhastSystem". Over 400 analyses were performed within the time of two years. Most patients were from nephrological, urological and kidney-transplant departments. Some of them were from obstetric, pediatric or oncological departments. A systematic discussion and evaluation of the two-dimensional protein pattern with typical examples is outlined.
Pathologisch-bakteriologisches Institut, Allgemeine Poliklinik der Stadt Wien, Austria.
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Lapin
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Feigl
D001798:Blood Proteins / Q000652:urine*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007674:Kidney Diseases / Q000652:urine*; D007677:Kidney Function Tests; D016030:Kidney Transplantation / Q000502:physiology; D011507:Proteinuria; D012016:Reference Values
D001798:Blood Proteins