Alteration in natural defense activity against NK-susceptible B16 melanoma cells after treatment with Corynebacterium parvum.
We have investigated the effects of administration of C. parvum on host anti-metastatic activity against B16 melanoma H-2L, a natural killer (NK) sensitive clone with a low expression of H-2b. The anti-metastatic activity was estimated by monitoring the following two points. One was the survival ratio at an early stage after an intravenous (iv) inoculation of radiolabeled B16 H-2L cells, the other was the formation of pulmonary metastases after iv injection with the tumor cells. Administration of C. parvum showed a biphasic change in the NK activity of the spleen cells and the peritoneal exudate cells (PEC) in mice. At an early phase (2-3 days) after administration of C. parvum, the NK activity of the spleen cells and PEC was significantly augmented. On the other hand, at a later phase (14 days) after C. parvum administration, the NK activity was deeply depressed. In correlation with NK activity of the mice treated with C. parvum, the anti-metastatic activity of the hosts was augmented in the early phase, whereas a depressed level of anti-metastatic activity was observed in the late phase after administration of C. parvum. These results suggest that the modification of NK activity is a possible basis for modulation of anti-metastatic activity by C. parvum.
Department of Immunology, Kyushu University, Fukuoka, Japan.
A
A
Karashima
K
K
Taniguchi
Y
Y
Yoshikai
K
K
Nomoto
D000818:Animals; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D005260:Female; D007114:Immunization; D007694:Killer Cells, Natural / Q000276:immunology*; D008175:Lung Neoplasms / Q000556:secondary*; D008545:Melanoma / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010529:Peritoneal Cavity / Q000166:cytology; D011425:Propionibacterium acnes / Q000276:immunology*; D012795:Sialoglycoproteins / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013997:Time Factors; D014407:Tumor Cells, Cultured
D012795:Sialoglycoproteins