Extracellular materials in the endothelial meshwork of organcultured human trabecular meshwork. Morphologic and morphometric study.
Twenty-three normal cadaver eyes were used in this study. Eight trabecular meshwork explants were dissected at 45-degree intervals from a 77-year-old donor eye and cultured for 2 weeks for studying circumferential differences in the amount of extracellular materials in the endothelial meshwork. One trabecular meshwork explant was dissected from each of 22 individual eyes (age range: 64-89 years) and cultured for 2 weeks to study the effect of age on the amount of the extracellular materials. Then, 5 consecutive electron micrographs in the endothelial meshwork were obtained from the individual specimens and subjected to morphometric studies. In the endothelial meshwork, the results revealed: (1) no statistically significant circumferential differences in the amount of extracellular materials and (2) no statistically significant changes associated with aging in the amount of these materials.
Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto, Japan.
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Urakawa
D002452:Cell Count; D016922:Cellular Senescence / Q000502:physiology; D004727:Endothelium / Q000166:cytology; D005109:Extracellular Matrix / Q000648:ultrastructure*; D006801:Humans; D009924:Organ Culture Techniques; D012016:Reference Values; D014129:Trabecular Meshwork / Q000166:cytology*