Effect of interleukin-1 on hyaluronate synthesis by synovial fibroblastic cells.
Interleukin-1 (IL-1) stimulates fibroblast-mediated hyaluronate (HA) synthesis in vitro. In the present study the degree of polymerization of such HA was studied using HPLC (high performance liquid chromatography) with a size exclusion column combined with 125I-HABP assay used to measure the HA concentration in various HA molecular weight fractions separated using HPLC. IL-1 stimulated HA was more polydisperse than that produced by resting fibroblasts with a molecular weight varying from more than 4 x 10(6) daltons to less than 7.1 x 10(3) daltons. This IL-1 effect may contribute to the low molecular weight HA produced by freshly explanted arthritic synovial tissue and to the low viscosity of arthritic synovial fluid in vivo.
Konttinen
Y T
YT
Fourth Department of Medicine, Helsinki University Central Hospital, Finland.
Saari
H
H
Nordström
D C
DC
D002851:Chromatography, High Pressure Liquid; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000494:pharmacology*; D008970:Molecular Weight; D011108:Polymers / Q000378:metabolism; D013582:Synovial Fluid / Q000166:cytology* / Q000187:drug effects
D007375:Interleukin-1; D011108:Polymers; D006820:Hyaluronic Acid