Biological monitoring of bidi rollers with respect to genotoxic hazards of occupational tobacco exposure.
Smokeless tobacco habits are associated with a high incidence of oropharyngeal cancer in India. Hence, the biological effects of occupational exposure to smokeless tobacco used for making bidis (the Indian version of cigarettes) were studied in 2 groups of bidi rollers designated BR-K and BR-S and in control subjects with no tobacco habits. Specific tobacco exposure and the electrophilic burden were determined by estimating urinary cotinine and thioethers respectively. Urine mutagenicity was tested with the Ames assay using Salmonella typhimurium strains TA98 and TA100. While cotinine was not detected in control samples, the mean cotinine levels (mmole/mole creatinine) in the BR-K and BR-S groups were 0.79 +/- 0.30 and 0.09 +/- 0.03 respectively. Urinary thioether excretion (mmole/mole creatinine) was significantly elevated in the BR-S group 4.59 +/- 0.52; p less than 0.001) but it was lower in the BR-K group (0.54 +/- 0.08; p less than 0.001) compared to the control (1.83 +/- 0.34). Furthermore, beta-glucuronidase-treated samples from both groups of bidi rollers exhibited increased mutagenicity to TA98 compared to the control group; in addition, BR-S samples exhibited direct mutagenicity to TA98. The results show that occupational tobacco exposure modulates the glutathione conjugation pathway and increases the mutagenic burden of bidi rollers.
Cancer Research Institute, Tata Memorial Centre, Bombay, India.
R A
RA
Bhisey
R B
RB
Govekar
D003367:Cotinine / Q000652:urine; D003404:Creatinine / Q000652:urine; D004305:Dose-Response Relationship, Drug; D004336:Drug Antagonism; D005260:Female; D005965:Glucuronates / Q000652:urine; D005966:Glucuronidase / Q000633:toxicity; D006801:Humans; D009152:Mutagenicity Tests; D009153:Mutagens; D016273:Occupational Exposure; D010947:Plants, Toxic; D012486:Salmonella typhimurium / Q000187:drug effects; D012977:Sodium Nitrite / Q000633:toxicity; D013440:Sulfides / Q000652:urine; D014030:Tobacco, Smokeless / Q000009:adverse effects*
D005965:Glucuronates; D009153:Mutagens; D013440:Sulfides; D003404:Creatinine; D005966:Glucuronidase; D003367:Cotinine; D012977:Sodium Nitrite