An interferon-induced 16-kD protein is present in the membranes of interferon-sensitive but not in interferon-resistant mouse cells.
Human interferon-alpha (IFN-alpha) or IFN-beta has been shown to induce a 17-kD membrane protein in human cells which when eluted from SDS gels inhibited the multiplication of cells of different human cell lines. We show herein that mouse IFN-alpha/beta induces a 16-kD membrane protein in L1210 and Friend erythroleukemia cells sensitive to IFN-alpha/beta, (but not in the derived IFN-alpha/beta-resistant cell lines) as well in primary and monolayer cultures of mouse embryonic fibroblasts and adult mouse hepatocytes, and in suspensions of spleen cells. In addition, IFN-alpha/beta enhanced the expression of an 11-kD membrane protein which could be shown by immunoprecipitation to be beta 2-microglobulin. Anticell proliferation activity was not recovered from the 16-kD fraction of the SDS gels.
Laboratory of Viral Oncology, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
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Vignaux
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Knight
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Eid
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Gresser
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000737:chemistry; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D008099:Liver / Q000166:cytology; D008565:Membrane Proteins / Q000737:chemistry*; D051379:Mice; D008970:Molecular Weight; D013154:Spleen / Q000166:cytology; D014407:Tumor Cells, Cultured; D001613:beta 2-Microglobulin / Q000378:metabolism
D007370:Interferon Type I; D008565:Membrane Proteins; D001613:beta 2-Microglobulin