Modification of alpha-amylase from Bacillus licheniformis by the polyaldehyde derived from beta-cyclodextrine and alpha-amylase thermostability.
The cleavage of beta-cyclodextrine by sodium periodate at the seven 2-3 diols of the glucose unit gives rise to the polyaldehyde 1, used to modify alpha-amylase. The reductive modification of alpha-amylase from Bacillus licheniformis reduced the number of reactive lysine groups from 8 to 3.5 per mol of enzyme with an activity loss of 25% and increased the half-life at 80 degrees C from 4.7 to 7.0 minutes.
URA-CNRS 31, Département de Chimie, Université Louis Pasteur, Strasbourg, France.
P
P
Morand
J F
JF
Biellmann
D000447:Aldehydes / Q000494:pharmacology*; D001407:Bacillus / Q000201:enzymology*; D003505:Cyclodextrins / Q000494:pharmacology*; D004795:Enzyme Stability; D000516:alpha-Amylases / Q000378:metabolism*; D047392:beta-Cyclodextrins
D000447:Aldehydes; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; D000516:alpha-Amylases; C031215:betadex