Immunohistochemical characterization of purple acid phosphatase-containing leucocytes in the human placenta.
A tartrate-resistant acid phosphatase activity was detected in the human placenta. This enzyme displayed immunological properties similar to those of the group of purple acid phosphatases that can be demonstrated with a rabbit polyclonal antibody against bovine spleen purple acid phosphatase. The placental enzyme was mainly localized immunohistochemically to neutrophil granulocytes of the maternal blood between the placental villi and within foetal capillaries using the bovine spleen antibody and the commercial monoclonal antibody M1 directed against an antigen found on mature granulocytes. A minor activity was detected in decidual cells and the syncytiotrophoblast. The presence of purple acid phosphatase in placental granulocytes may be related to special immunological conditions of pregnancy.
Institute of Anatomy, University of Münster, Germany.
J
J
Schindelmeiser
D
D
Münstermann
D000135:Acid Phosphatase / Q000032:analysis*; D006098:Granulocytes / Q000201:enzymology*; D006801:Humans; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000201:enzymology*; D010920:Placenta / Q000166:cytology*; D013644:Tartrates
D013644:Tartrates; D000135:Acid Phosphatase; C029768:tartaric acid