Cyclic adenosine 3',5'-monophosphate (cAMP)-dependent protein kinase activity in the somatic cells of the seminiferous tubules. I. Isoform analysis.
In this work firstly are reported the chromatographic analysis of cAMP dependent protein kinases present in the cytosols obtained from rat Sertoli cells and peritubular cells. In both cell types two different isoenzymes have been detected, one eluting at 40-80 mM KC1 (type I) and a second one eluting at 150-200 mM KC1 (type II). Only the type I was strongly stimulated by cAMP whereas the type II was slightly cAMP dependent both in the Sertoli cells and in the peritubular cells.
Istituto di Istologia ed Embriologia generale, Università degli Studi di Roma La Sapienza, Italila.
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Nistico
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Pezzotti
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Galdieri
D000818:Animals; D002478:Cells, Cultured; D003600:Cytosol / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012671:Seminiferous Tubules / Q000166:cytology / Q000201:enzymology*; D012708:Sertoli Cells / Q000201:enzymology; D013087:Spermatids / Q000201:enzymology
D007527:Isoenzymes; D011494:Protein Kinases