Effect of Plasmodium berghei infection and chloroquine on the hepatic drug metabolizing system of mice.
The hepatic microsomal mixed-function oxidase (MFO) system was markedly impaired during Plasmodium berghei infection in mice. Cytochrome P-450 and other mono-oxygenases, viz. aniline hydroxylase, aminopyrine-N-demethylase and benzo(a)pyrene hydroxylase, were significantly decreased while microsomal heme showed a four-fold increase at peak parasitemia (greater than 50%). Oral treatment with chloroquine (16 mg kg-1 body wt for 4 days) of P. berghei-infected mice cleared the parasitemia within 72 h and almost normalized the altered levels of MFO indices, a week after cessation of treatment. The findings were further supported by the isoenzymic profile and drug-binding properties of terminal mono-oxygenase, cytochrome P-450.
Srivastava
P
P
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Tripathi
L M
LM
Puri
S K
SK
Dutta
G P
GP
Pandey
V C
VC
D000818:Animals; D002738:Chloroquine / Q000378:metabolism / Q000627:therapeutic use*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D007527:Isoenzymes; D008288:Malaria / Q000188:drug therapy / Q000201:enzymology*; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010962:Plasmodium berghei
D007527:Isoenzymes; D002738:Chloroquine; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases