An electrophoretic method to evaluate DNA polymerase activity.
We have devised an easy non-radioactive assay to evaluate the activity of six different DNA polymerases: the Klenow fragment of E.coli DNA Polymerase I, Taq DNA Polymerase, Sequenase, Moloney Murine Reverse Transcriptase, T7 DNA Polymerase and E.coli DNA Polymerase I. The method is based on the differential mobility of single stranded DNA compared to that of double-stranded DNA in agarose gel electrophoresis.
Ranganathan
R
R
Department of Molecular Biology, Brockwood Park Educational Centre, Bramdean, Hampshire, UK.
Calvo-Riera
F
F
D004247:DNA / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000379:methods
D004277:DNA, Single-Stranded; D004247:DNA; D004259:DNA-Directed DNA Polymerase