MM 55266 and MM 55268, glycopeptide antibiotics produced by a new strain of Amycolatopsis. Isolation, purification and structure determination.
Two novel glycopeptide antibiotics MM 55266 and MM 55268 containing fatty acid acyl functions, and of molecular formula C86H89N8O35Cl5 and C87H91N8O35Cl5, respectively, have been isolated and identified from a complex produced by Amycolatopsis sp. NCIB 40089. Fermentation conditions for their production, and methods for their isolation are described. Structures have been deduced by use of COSY and NOE NMR techniques and supported by chemical degradation studies. Both glycopeptides possessed good antibacterial activity against Gram-positive organisms.
Box
S J
SJ
SmithKline Beeham Pharmaceuticals, Chemotherapeutic Research Centre, Betchworth, Surrey, UK.
Coates
N J
NJ
Davis
C J
CJ
Gilpin
M L
ML
Houge-Frydrych
C S
CS
Milner
P H
PH
D000192:Actinomycetales / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D006020:Glycopeptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D012310:Ristocetin / Q000031:analogs & derivatives; D013329:Structure-Activity Relationship
D000900:Anti-Bacterial Agents; D006020:Glycopeptides; C071010:MM 55268; C071009:MM 55266; D012310:Ristocetin