The development of a treatment model for lymph node metastasis in nude mice transplanted with rat fibrosarcoma clone A.
We have tried to establish a model for lymph node metastasis in nude mice transplanted with clone A of methylcholanthrene-induced rat fibrosarcoma. Tumor cells transplanted either subcutaneously or intrafootpad metastasized exclusively to the lymph nodes in nude mice treated with antiasialo GM1 antiserum. In the experiments of intrafootpad transplantation, all the mice died of disseminated lymph node metastasis if the leg was not resected, or was resected more than 3 weeks after the transplantation of clone A. Some mice whose legs were amputated 1 or 2 weeks after transplantation could be cured by amputation alone. The maximum effective dose of in vitro active drug could improve the survival in mice, and 3/5, 4/5, and 3/5 mice treated with MMC, ACNU and CDDP respectively were cured even if their legs were amputated 3 weeks after transplantation. This tumor-bearing animal system was considered to provide a valuable experimental model for lymph node metastasis.
Pharmacology Division, National Cancer Center Research Institute, Tokyo, Japan.
Y
Y
Soejima
W M
WM
Shen
K
K
Minato
K
K
Nishio
Y
Y
Sugimoto
Y
Y
Fujiwara
T
T
Tamura
K
K
Hara
N
N
Saijo
D000671:Amputation, Surgical; D000818:Animals; D003131:Combined Modality Therapy; D005354:Fibrosarcoma / Q000473:pathology* / Q000556:secondary / Q000628:therapy; D008207:Lymphatic Metastasis / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016685:Mitomycin / Q000627:therapeutic use; D009368:Neoplasm Transplantation; D051381:Rats
D016685:Mitomycin