Effect of interleukin-1 on hyaluronate synthesis by synovial fibroblastic cells.
Interleukin-1 (IL-1) stimulates fibroblast-mediated hyaluronate (HA) synthesis in vitro. In the present study the degree of polymerization of such HA was studied using HPLC (high performance liquid chromatography) with a size exclusion column combined with 125I-HABP assay used to measure the HA concentration in various HA molecular weight fractions separated using HPLC. IL-1 stimulated HA was more polydisperse than that produced by resting fibroblasts with a molecular weight varying from more than 4 x 10(6) daltons to less than 7.1 x 10(3) daltons. This IL-1 effect may contribute to the low molecular weight HA produced by freshly explanted arthritic synovial tissue and to the low viscosity of arthritic synovial fluid in vivo.
Fourth Department of Medicine, Helsinki University Central Hospital, Finland.
Y T
YT
Konttinen
H
H
Saari
D C
DC
Nordström
D002851:Chromatography, High Pressure Liquid; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000494:pharmacology*; D008970:Molecular Weight; D011108:Polymers / Q000378:metabolism; D013582:Synovial Fluid / Q000166:cytology* / Q000187:drug effects
D007375:Interleukin-1; D011108:Polymers; D006820:Hyaluronic Acid