Isolation of mutants blocked in calicheamicin biosynthesis.
Blocked mutants of Micromonospora echinospora defective in the production of the potent antitumor agent calicheamicin are described. Analysis of intermediates produced by blocked mutants indicates a branched biosynthetic pathway. Mutants that produced some, but not all, calicheamicin forms are also described.
Department of Microbial Genetics, Lederle Laboratories, Pearl River, New York 10965.
D M
DM
Rothstein
S F
SF
Love
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis*; D008847:Micromonospora / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D015394:Molecular Structure; D016296:Mutagenesis; D013045:Species Specificity; D013329:Structure-Activity Relationship
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic