Mode of membrane insertion and sequence of a 32-amino acid peptide stretch of the penicillin-binding protein 4 of Enterococcus hirae.
Analysis of water-soluble derivatives of the Enterococcus hirae 75-kDa membrane-bound penicillin-binding protein 4 (PBP4) has yielded the amino acid sequence of a 32-amino acid polypeptide stretch. This peptide is similar to peptide segments known to occur in the N-terminal domain of high-Mr PBPs of class B. The E. hirae PBP4 probably belongs to the same class. It is anchored in the membrane at the N-terminus of the polypeptide chain.
Centre d'Ingénierie des Protéines, Université de Liège, Belgium.
P
P
Jacques
A
A
el Kharroubi
J
J
Van Beeumen
G
G
Piras
J
J
Coyette
J M
JM
Ghuysen
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D016983:Enterococcus / Q000378:metabolism*; D006602:Hexosyltransferases; D008565:Membrane Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D002267:Muramoylpentapeptide Carboxypeptidase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D046915:Penicillin-Binding Proteins; D010446:Peptide Fragments / Q000378:metabolism*; D010458:Peptidyl Transferases; D014357:Trypsin / Q000378:metabolism
D001426:Bacterial Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; D046915:Penicillin-Binding Proteins; D010446:Peptide Fragments; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D014357:Trypsin