[An evaluation of the conformational changes in the superhelical DNA of eukaryotic cells by direct nucleoid fluorometry. II. The characteristics of the change in acridine orange fluorescence in studying the superhelical DNA of rat thymocytes].
Comparative studies of acridine orange (AO) and ethidium bromide interactions with supercoiled DNA (scDNA) of thymocytes were performed in which various conformational changes were induced. AO may be efficiently used for evaluation of conformational alterations of scDNA. Moreover, employing the maximum values of AO fluorescence allows to determine the maximum levels of scDNA relaxation.
Reshchikov
A M
AM
Vashchenko
V I
VI
Komar
V E
VE
D000165:Acridine Orange; D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D004278:DNA, Superhelical / Q000648:ultrastructure*; D004996:Ethidium; D005434:Flow Cytometry; D005453:Fluorescence; D007364:Intercalating Agents; D008214:Lymphocytes / Q000648:ultrastructure; D009690:Nucleic Acid Conformation; D051381:Rats; D013950:Thymus Gland / Q000648:ultrastructure*; D014783:Viscosity
D004278:DNA, Superhelical; D007364:Intercalating Agents; D004996:Ethidium; D000165:Acridine Orange
Otsenka konformatsionnykh izmeneniĭ superspiral'noĭ DNK éukarioticheskikh kletok metodom priamoĭ fluorimetrii nukleoidov. II. Osobennosti izmeneniia fluorestsentsii akridinovogo oranzhevogo pri izuchenii superspiral'noĭ DNK timotsitov krys.