p-nitrophenyllaurate: a substrate for the high-performance liquid chromatographic determination of lipase activity.
Many assay procedures have been devised to measure lipolytic activity, but none is without problems. It is for this reason that new methods are still being proposed. In this work we have investigated the use of two esters of p-nitrophenol, the palmitic acid and lauric acid esters, as substrates for a highly sensitive high-performance liquid chromatographic method. Data on recovery, specific activity and reproducibility are reported only for the lauric ester, because the palmitic ester turned out to be a very poor substrate.
Maurich
V
V
Department of Pharmaceutical Science, University of Trieste, Italy.
Zacchigna
M
M
Pitotti
A
A
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007700:Kinetics; D007848:Laurates / Q000737:chemistry* / Q000378:metabolism; D007850:Lauric Acids / Q000378:metabolism; D008049:Lipase / Q000378:metabolism*; D009596:Nitrophenols / Q000378:metabolism; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D015203:Reproducibility of Results; D013379:Substrate Specificity
D007848:Laurates; D007850:Lauric Acids; D009596:Nitrophenols; D010169:Palmitic Acids; C030358:lauric acid; C050014:4-nitrophenyl laurate; D019308:Palmitic Acid; D008049:Lipase