Characterization of a tissue-type plasminogen activator from porcine urine.
Porcine urine, unlike human urine, does not contain detectable amounts of urokinase-type plasminogen activator (u-PA). The plasminogen activator present in porcine urine is of tissue-type (t-PA) as identified by the following criteria. (1) Porcine urine PA exhibits an Mr of 65,000 similar to the Mr of human t-PA (64-70,000) but distinct from the Mr of human u-PA (55,000). (2) Antibodies against human t-PA bind and inhibit crude and purified porcine urine PA, while human u-PA-specific antibodies do not react with porcine urine PA. (3) Plasminogen activation by porcine urine PA is markedly stimulated in the presence of fibrinogen fragments. (4) Porcine urine PA activity is not affected by concentration of amiloride substantially suppressing human u-PA activity.
Department of Enzymology, Central Institute of Molecular Biology, Berlin, Germany.
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D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D004792:Enzyme Precursors / Q000652:urine; D005260:Female; D006801:Humans; D015151:Immunoblotting; D008297:Male; D010958:Plasminogen / Q000378:metabolism; D013552:Swine; D010959:Tissue Plasminogen Activator / Q000302:isolation & purification / Q000494:pharmacology / Q000652:urine*; D014568:Urokinase-Type Plasminogen Activator / Q000302:isolation & purification / Q000494:pharmacology / Q000652:urine
D000906:Antibodies; D000911:Antibodies, Monoclonal; D004792:Enzyme Precursors; D000584:Amiloride; D010958:Plasminogen; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator