Imidazole binding sites in rabbit kidney and forebrain membranes.
1. The binding of [3H]-clonidine, [3H]-idazoxan and [3H]-yohimbine to rabbit forebrain and kidney membranes was compared. 2. Yohimbine bound exclusively to adrenergic sites, idazoxan to non-adrenergic sites and clonidine to both non-adrenergic and adrenergic sites. 3. Differences were observed between the ligands not only in binding at adrenergic and non-adrenergic sites but also between the non-adrenergic binding of [3H]-clonidine and [3H]-idazoxan. 4. However, no tissue specific differences were found.
University Department of Medicine and Therapeutics, Stobhill General Hospital, Glasgow, Scotland.
C A
CA
Hamilton
M A
MA
Yakubu
E
E
Jardine
J L
JL
Reid
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D003000:Clonidine / Q000378:metabolism; D004146:Dioxanes / Q000378:metabolism; D019329:Idazoxan; D007093:Imidazoles / Q000378:metabolism*; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D009638:Norepinephrine / Q000378:metabolism; D010275:Parasympathetic Nervous System / Q000378:metabolism; D011817:Rabbits; D013564:Sympathetic Nervous System / Q000378:metabolism; D015016:Yohimbine / Q000378:metabolism
D004146:Dioxanes; D007093:Imidazoles; D015016:Yohimbine; D003000:Clonidine; D009638:Norepinephrine; D019329:Idazoxan