Bradykinin induces eosinophil accumulation in the rat pleural cavity.
Intrathoracic injections of bradykinin (1-100 micrograms/cavity) induced a dose-dependent increase in the number of eosinophils recovered from the rat pleural cavity 24 h later. Eosinophilia by bradykinin was preceded by a marked pleural neutrophil influx within 6 h and was absent only 72 h following stimulation. Bradykinin (10(-9)-10(-5) M) failed to induce in vitro eosinophil chemotaxis, indicating that its in vivo effect must be mediated by an intermediate messenger. BW 755C (25 mg/kg) and the more selective lipoxygenase inhibitor BW A4C (20 micrograms/cavity) suppressed the pleural eosinophilia induced by bradykinin (50 micrograms/cavity), whereas the platelet-activating factor (PAF)-acether antagonist BN 52021 was inactive. We conclude that bradykinin is able to attract eosinophil in vivo by a mechanism independent of PAF-acether and sensitive to the blockage of the lipoxygenase pathway.
Pasquale
C P
CP
Departamento de Fisiologia e Farmacodinâmica, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
Martins
M A
MA
Bozza
P T
PT
Silva
P M
PM
Faria Neto
H C
HC
Pires
A L
AL
Cordeiro
R S
RS
D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine / Q000494:pharmacology; D000818:Animals; D044482:Benzeneacetamides; D001920:Bradykinin / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects*; D002633:Chemotaxis; D004224:Diterpenes; D004305:Dose-Response Relationship, Drug; D004802:Eosinophilia / Q000139:chemically induced / Q000473:pathology*; D005260:Female; D046934:Ginkgolides; D006877:Hydroxamic Acids / Q000494:pharmacology; D007274:Injections, Intraperitoneal; D007783:Lactones / Q000494:pharmacology; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D008297:Male; D009504:Neutrophils / Q000502:physiology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology; D010994:Pleura / Q000276:immunology*; D010998:Pleurisy / Q000209:etiology; D051381:Rats; D011919:Rats, Inbred Strains
D044482:Benzeneacetamides; D004224:Diterpenes; D046934:Ginkgolides; D006877:Hydroxamic Acids; D007783:Lactones; D016859:Lipoxygenase Inhibitors; D010972:Platelet Activating Factor; C056614:N-(3-phenoxycinnamyl)acetohydroxamic acid; D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; C045856:ginkgolide B; D001920:Bradykinin