[The effect of neutral fraction of cigarette smoke condensate on DNA content of hamster's tracheal epithelial cells].
Histologic changes and DNA content alterations of hamster's tracheal epithelial cells induced by Benzo(a)pyrene[B(a)P] and/or the neutral fraction of cigarette smoke condensate (CSC-NF) in organ culture were investigated by light microscopy and automated image analysis technique. The results showed that B(a)P + CSC-NF induced severe atypical hyperplasia of the epithelium. Additionally, the DNA content and the percentage of cells with aneuploid DNA content also increased significantly. The histogram of DNA distribution became apparently widened. The high peak was shifted to the right and many irregular median and low peaks were also obtained.
Department of Pathology, China Medical University.
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D000782:Aneuploidy; D000818:Animals; D001564:Benzo(a)pyrene; D003043:Cocarcinogenesis; D006224:Cricetinae; D046508:Culture Techniques; D004247:DNA / Q000032:analysis* / Q000235:genetics; D004848:Epithelium / Q000187:drug effects; D007091:Image Processing, Computer-Assisted; D008297:Male; D008647:Mesocricetus; D010947:Plants, Toxic; D012906:Smoke / Q000009:adverse effects*; D013638:Tars / Q000009:adverse effects*; D014026:Nicotiana / Q000737:chemistry; D014132:Trachea / Q000187:drug effects*
D012906:Smoke; D013638:Tars; D001564:Benzo(a)pyrene; D004247:DNA