Molecular cloning and characterization of two distinct hsp85 sequences from the steroid responsive fungus Achlya ambisexualis.
In Achlya ambisexualis, hsp85 is one of the characteristic mycelial heat shock proteins induced in response to a rapid elevation in temperature (Silver et al. 1983). This heat shock protein has the same electrophoretic mobility on two-dimensional gels and is antigenically related to an 85 kDa steroid hormone-regulated protein which constitutes a component of the putative Achlya steroid hormone-receptor complex. We report here the isolation of two distinct, yet highly related, hsp85 gene sequences from Achlya genomic libraries. Northern analyses, using these two Achlya genomic sequences as probes, suggest that there are two hsp85 message population in Achlya and that at least one of these is regulated by the steroid hormone antheridiol.
Department of Microbiology, University of Toronto, Ontario, Canada.
S A
SA
Brunt
J C
JC
Silver
D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D004330:Drosophila / Q000235:genetics; D005658:Fungi / Q000187:drug effects / Q000235:genetics*; D005800:Genes, Fungal; D006360:Heat-Shock Proteins / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics; D015183:Restriction Mapping; D013256:Steroids / Q000494:pharmacology*; D014158:Transcription, Genetic
D004271:DNA, Fungal; D006360:Heat-Shock Proteins; D012331:RNA, Fungal; D013256:Steroids