Deoxyribonuclease activities in Myxococcus coralloides D.
Myxococcus coralloides D produced cell-bound deoxyribonucleases (DNases) during the exponential phase of growth in liquid medium. DNase activity was much higher than that detected in other myxobacterial strains and was fractionated into three different peaks by filtration through Sephadex G-200. The DNases were named G, M and P. The optimum temperatures were 37 degrees C, 33 degrees C and 25 degrees C respectively, although high activities were recorded over the temperature range 20-45 degrees C. The pH range of high activity was between 6.0 and 9.0, with an optimum for each DNase at 8.0. DNases M and P were strongly inhibited by low concentrations of NaCl, but activity of DNase G was less affected by NaCl. The three activities required divalent metal ions as cofactors (especially Mg2+ and Mn2+); however, other metal ions (Fe2+, Ni2+, Zn2+) were inhibitors. The molecular weights were estimated by gel filtration chromatography and SDS-PAGE as 44 kDa (DNase G), 49 kDa (DNase M) and 39 kDa (DNase P).
Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, Spain.
M M
MM
Martínez-Cañamero
J
J
Muñoz
A L
AL
Extremera
J M
JM
Arias
D002850:Chromatography, Gel; D003851:Deoxyribonucleases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008970:Molecular Weight; D016940:Myxococcus / Q000201:enzymology*; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature
D008345:Manganese; D012965:Sodium Chloride; D003851:Deoxyribonucleases; D008274:Magnesium