Gel electrophoresis of polystyrene particles in glutaraldehyde crosslinked polyvinyl alcohol.
Polystyrene sulfate and carboxylate particles (19-189 nm radius) were subjected to electrophoresis in glutaraldehyde crosslinked polyvinyl alcohol of molecular weight 25.000 and 650.000 Da at various concentrations. The degree of crosslinking is severely limited by the mechanical properties of the gels that deteriorate beyond a glutaraldehyde concentration which decreases with increasing polyvinyl alcohol chain length. The effective fiber radius of the short-chain and long-chain polymer fiber was 45 +/- 25 and 131 +/- 47 nm, respectively. Thus, these media do not significantly exceed the apparent fiber thickness of agarose, are more difficult to prepare--but are well-defined synthetic products rather than natural ones, and have the advantage of carrying no net charge and can therefore be expected to exhibit no electroendosmosis.
Section on Macromolecular Analysis, Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
J
J
Pospichal
D
D
Tietz
T R
TR
Ittyerah
D
D
Halpern
A
A
Chrambach
D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004586:Electrophoresis / Q000379:methods*; D005976:Glutaral / Q000494:pharmacology*; D008863:Microspheres; D008970:Molecular Weight; D010316:Particle Size; D011137:Polystyrenes / Q000302:isolation & purification*; D011142:Polyvinyl Alcohol
D003432:Cross-Linking Reagents; D011137:Polystyrenes; D011142:Polyvinyl Alcohol; D005976:Glutaral