Comparison of ultracentrifugation and polyethylene glycol precipitation for concentration of hepatitis B virus (HBV) DNA for molecular hybridisation tests and the relationship of HBV-DNA to HBe antigen and anti-HBe status.
A 32P-labelled DNA probe was used to examine 50 hepatitis B surface antigen (HBsAg)-positive sera for the presence of hepatitis B virus (HBV) DNA. HBV-DNA was detected in all 21 HBeAg-positive samples, in one out of 21 anti-HBe-positive samples and in three out of eight HBeAg- and anti-HBe-negative samples. The results of this DNA hybridisation test correlated well with HBeAg status and could be used to determine infectivity in HBeAg- and anti-HBe-negative samples. Ultracentrifugation was marginally superior to polyethylene glycol precipitation for concentrating HBV-DNA from serum.
Public Health Laboratory, Northern General Hospital, Sheffield.
D
D
Sanyal
G
G
Kudesia
G
G
Corbitt
D011232:Chemical Precipitation; D003101:Collodion; D004279:DNA, Viral / Q000097:blood*; D006509:Hepatitis B / Q000382:microbiology; D006513:Hepatitis B e Antigens / Q000235:genetics* / Q000276:immunology; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D009693:Nucleic Acid Hybridization; D011092:Polyethylene Glycols; D014461:Ultracentrifugation
D004279:DNA, Viral; D006513:Hepatitis B e Antigens; D011092:Polyethylene Glycols; D003101:Collodion