Baculovirus-mediated expression of human apolipoprotein E in Manduca sexta larvae generates particles that bind to the low density lipoprotein receptor.
Human apolipoprotein E (apoE) is a ligand for the low density lipoprotein (LDL) receptor and mediates the catabolism of several classes of lipoprotein particles. Binding of apoE to the LDL receptor requires association of apoE with lipid in a vesicle or a lipoprotein particle. Because of this requirement, purified apoE or apoE derived directly from bacterial expression systems does not bind to the LDL receptor. To overcome this problem and to facilitate analysis of apoE structure, recombinant baculoviruses containing the human apoE cDNA fused to the polyhedrin promoter of Autographa californica nuclear polyhedrosis virus were constructed. The recombinant viruses were used to infect larvae of the tobacco hornworm Manduca sexta in vivo. High levels of lipoprotein particles containing human apoE were present in the hemolymph of infected larvae. In contrast to apoE produced by recombinant baculovirus-infected insect cells in vitro, these particles were excellent ligands for the LDL receptor.
Gretch
D G
DG
Department of Biochemistry, University of Wisconsin-Madison 53706.
Sturley
S L
SL
Friesen
P D
PD
Beckage
N E
NE
Attie
A D
AD
D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics* / Q000378:metabolism; D016367:Baculoviridae / Q000235:genetics; D015153:Blotting, Western; D003001:Cloning, Molecular; D005822:Genetic Vectors; D006458:Hemolymph / Q000378:metabolism; D006801:Humans; D007814:Larva; D050356:Lipid Metabolism; D008970:Molecular Weight; D009036:Moths; D011973:Receptors, LDL / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism
D001057:Apolipoproteins E; D011973:Receptors, LDL; D011994:Recombinant Proteins