The in vitro and in vivo inhibition of protein glycosylation and diabetic vascular basement membrane thickening by pyridoxal-5'-phosphate.
We studied the effect of pyridoxal-5'-phosphate (PAL-P) on protein glycosylation and diabetic nephropathy in NSY mice. In experiment 1, an in vitro model of the browning phenomenon involving the incubation of lysine and glucose was inhibited by PAL-P. In experiment 2, administration of PAL-P to congenitally diabetic NSY mice markedly reduced the thickening of the glomerular basement membrane. These results suggest that PAL-P has the potential to be used for reducing the nephrotic complications of diabetes mellitus.
Nagoya Quarantine Station, Yokkaichi Branch Office, Ministry of Health and Welfare, Yokkaichi, Japan.
M
M
Hayakawa
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M
Shibata
D000818:Animals; D001485:Basement Membrane / Q000187:drug effects / Q000648:ultrastructure; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D003928:Diabetic Nephropathies / Q000151:congenital / Q000473:pathology*; D019270:Fructosamine; D006442:Glycated Hemoglobin / Q000378:metabolism; D006031:Glycosylation / Q000187:drug effects; D006595:Hexosamines / Q000097:blood; D066298:In Vitro Techniques; D007678:Kidney Glomerulus / Q000098:blood supply* / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011732:Pyridoxal Phosphate / Q000494:pharmacology*
D001786:Blood Glucose; D019270:Fructosamine; D006442:Glycated Hemoglobin; D006595:Hexosamines; D011732:Pyridoxal Phosphate