Identification of related DNA sequences in Borrelia burgdorferi and two strains of Leptospira interrogans by using polymerase chain reaction.
The suitability of a polymerase chain reaction assay for Borrelia burgdorferi in epidemiological studies of infected tick populations was evaluated by using 28 strains of Leptospira interrogans and lysates of fixed adult Ixodes tick tissues. Two false positives representing leptospires were differentiated from B. burgdorferi by using an oligonucleotide probe.
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MA
Department of Pathology, College of Human Medicine, Michigan State University, East Lansing 48824.
Gupta
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A
Mackenzie
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D000818:Animals; D001483:Base Sequence; D015748:Borrelia burgdorferi Group / Q000235:genetics*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics*; D007921:Leptospira interrogans / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013987:Ticks / Q000382:microbiology
D015342:DNA Probes; D004269:DNA, Bacterial