Interleukin-5 mRNA in mucosal bronchial biopsies from asthmatic subjects.
Using in situ hybridization, we have investigated the expression of interleukin-5 (IL-5) mRNA in bronchial biopsies from asthmatics (n = 10) and controls (n = 9). The number of IL-5-nRNA-positive cells were compared with the number of CD25+ and EG2+ cells and total eosinophil counts. Specific hybridization signals for IL-5 mRNA were demonstrated in 6 out of the 10 asthmatic subjects but in none of the controls. The 6 IL-5-mRNA-positive asthmatics tended to have more severe disease and showed a significant increase in the degree of infiltration of the bronchial mucosa by activated T lymphocytes and eosinophils.
Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, UK.
Q
Q
Hamid
M
M
Azzawi
S
S
Ying
R
R
Moqbel
A J
AJ
Wardlaw
C J
CJ
Corrigan
B
B
Bradley
S R
SR
Durham
J V
JV
Collins
P K
PK
Jeffery
D001249:Asthma / Q000378:metabolism*; D001706:Biopsy; D001980:Bronchi / Q000737:chemistry*; D006801:Humans; D015848:Interleukin-5 / Q000235:genetics*; D009092:Mucous Membrane / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis*
D015848:Interleukin-5; D012333:RNA, Messenger