Enzymatic method for measuring ethylene glycol with a centrifugal analyzer.
We describe a semiautomated, enzymatic method of analysis for ethylene glycol in plasma. Glycerol dehydrogenase (EC 1.1.1.6) from Enterobacter aerogenes, which has high specificity for ethylene glycol, is mixed with 3 microL of specimen in a centrifugal analyzer. NAD+ is added to initiate the reaction, and 450 s after an initial 90-s delay, the absorbance at 340 nm is measured. Monthly calibration with a five-point calibration curve is sufficient to provide between-day precision (CV) of 2% to 5%. The detection limit is 0.3 mmol/L with a CV of 10%. There is no significant interference from ethanol, methanol, isopropanol, acetone, lactate, or glycerol.
Department of Pathology, University of New Mexico School of Medicine, Albuquerque 87131.
J
J
Standefer
W
W
Blackwell
D002498:Centrifugation; D019855:Ethylene Glycol; D005026:Ethylene Glycols / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques
D005026:Ethylene Glycols; D019855:Ethylene Glycol