The generation of a library of PCR-analyzed microsatellite variants for genetic mapping of the mouse genome.
Forty-three sequences containing simple sequence repeats or microsatellites were generated from an M13 library of total genomic mouse DNA. These sequences were analyzed for size variation using the polymerase chain reaction and gel electrophoresis without the need for radiolabeling. Seventy-two percent of the sequences showed allelic size variations between different inbred strains of mouse and the wild mouse, Mus spretus; and 53% showed variation between inbred strains. Thirty-seven percent were variant between B6/J and DBA/2J, and 81% of these were resolved using minigel agarose electrophoresis alone. This approach is a useful way of generating the large number of variants that are needed to create high resolution maps of the mouse genome.
Nuffield Department of Surgery, John Radcliffe Hospital, Headington, Oxford, United Kingdom.
R J
RJ
Cornall
T J
TJ
Aitman
C M
CM
Hearne
J A
JA
Todd
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004276:DNA, Satellite / Q000235:genetics*; D015723:Gene Library; D014644:Genetic Variation; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D008969:Molecular Sequence Data; D009115:Muridae / Q000235:genetics*; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D012091:Repetitive Sequences, Nucleic Acid
D004276:DNA, Satellite; D009838:Oligodeoxyribonucleotides