Automated separation and quantitation of lipid fractions by high-performance liquid chromatography and mass detection.
This report describes an improved separation and quantitation of lipid fractions in a total lipid extract by high-performance liquid chromatography using a modified solvent and gradient system delivered by dual pumps and incorporating a mass detector and autosampler. The detector responses for various lipid fractions (cholesteryl esters, triacylglycerols, free cholesterol, and seven major phospholipid classes) were fitted to a quadratic equation, y = ax2 + bx + c, and quantified after detector calibration by a computer. This new system has the advantage of automation and reproducible separation. The present method was applied to rat liver analysis.
Efamol Research Institute, Kentville, Nova Scotia, Canada.
P R
PR
Redden
Y S
YS
Huang
D000818:Animals; D002784:Cholesterol / Q000032:analysis / Q000302:isolation & purification; D002788:Cholesterol Esters / Q000032:analysis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D008055:Lipids / Q000032:analysis / Q000302:isolation & purification*; D008099:Liver / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000032:analysis / Q000302:isolation & purification
D002788:Cholesterol Esters; D008055:Lipids; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol