Intrinsic fluorescence of chloramphenicol acetyltransferase: responses to ligand binding and assignment of the contributions of tryptophan residues by site-directed mutagenesis.
Replacement by tyrosine or phenylalanine was used to assign the additive contributions of each of the three tryptophan residues of chloramphenicol acetyltransferase (CAT) to its intrinsic fluorescence on excitation at 295 nm. During the assessment of the fluorescence responses of the wild-type enzyme to the binding of ligands, it was found that the overlapping absorption spectra of chloramphenicol and tryptophan, with an attendant inner filter effect, required the use of a displacement technique involving an alternative substrate (the p-cyano analogue of chloramphenicol) without significant absorption at 295 nm. By the use of two-Trp, one-Trp, and Trp-less variants, in combination with this displacement technique, it was possible to demonstrate that Trp-86 and Trp-152 are involved in the fluorescence quenching associated with the binding of chloramphenicol, most likely via nonradiative energy transfer from these residues to the bound substrate. Trp-152 is mainly responsible for the fluorescence enhancement accompanying the binding of acetyl-CoA (and CoA) through proximity effects and solvent exclusion on substrate association.
Department of Biochemistry, University of Leicester, U.K.
J
J
Ellis
I A
IA
Murray
W V
WV
Shaw
D000105:Acetyl Coenzyme A / Q000378:metabolism; D002701:Chloramphenicol / Q000378:metabolism; D015500:Chloramphenicol O-Acetyltransferase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005453:Fluorescence; D015870:Gene Expression; D006358:Hot Temperature; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D013050:Spectrometry, Fluorescence; D014162:Transfection; D014364:Tryptophan / Q000737:chemistry*
D002701:Chloramphenicol; D000105:Acetyl Coenzyme A; D014364:Tryptophan; D015500:Chloramphenicol O-Acetyltransferase