Quantitation of scleral tobramycin levels.
We have devised a new method to solubilize sclera in order to permit quantitative determination of tobramycin by commercially available and widely utilized means. The sclera, containing tobramycin, was digested with Alcalase (Subtilisn A, NOVO Enzymes) and the tobramycin level was quantitated by TDX autoflourophotometry. This technique is accurate and sensitive. The tissue digestion and antibiotic assay are relatively simple to perform and should be applicable to a variety of antibiotics and possibly other therapeutic agents.
Department of Ophthalmology, University of Arizona College of Medicine, Tucson.
R W
RW
Snyder
C J
CJ
Gualtieri
J C
JC
Jahnke
P B
PB
Hemmady
M L
ML
McDermott
D000818:Animals; D015590:Fluorophotometry; D011817:Rabbits; D012590:Sclera / Q000737:chemistry* / Q000378:metabolism; D012995:Solubility; D014031:Tobramycin / Q000032:analysis* / Q000493:pharmacokinetics
D014031:Tobramycin