Purification and biochemical characterization of dog gastric lipase.
A lipase was found to be present in dog stomach which appeared to be more abundant in the fundic than in the pyloric mucosa. Dog gastric lipase was extracted by soaking the gastric tissue and further purified after cation exchange, anion exchange and gel-filtration using fast protein liquid chromatography. The amino-acid composition, N-terminal amino-acid sequence, substrate specificity, interfacial and kinetic behavior and inactivation by sulfhydryl reagents were determined and compared with those of human and rabbit gastric lipases. We report for the first time that a gastric lipase is 13 times more active on long-chain than on short-chain triacylglycerols at pH 4.0, reaching a maximal specific activity of 950 U/mg on Intralipide emulsion.
Centre de Biochimie et de Biologie Moléculaire du Centre National de la Recherche Scientifique, Marseille, France.
F
F
Carrière
H
H
Moreau
V
V
Raphel
R
R
Laugier
C
C
Benicourt
J L
JL
Junien
R
R
Verger
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005217:Fat Emulsions, Intravenous / Q000378:metabolism; D005753:Gastric Mucosa / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D013379:Substrate Specificity; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D014280:Triglycerides / Q000378:metabolism
D000596:Amino Acids; D005217:Fat Emulsions, Intravenous; D013439:Sulfhydryl Reagents; D014280:Triglycerides; D008049:Lipase