Comparison of a fluorimetric and a colorimetric method for the determination of hydrogen peroxide production by rat pleural macrophages.
A fluorimetric and a colorimetric method for the determination of hydrogen peroxide (H2O2) production by isolated cells were compared. Despite a higher sensitivity of the fluorimetric assay, using homovanillic acid (HVA) as reagent, a significantly lower H2O2 production by rat pleural macrophages was measured in comparison to the colorimetric phenol red method. A negative influence of HVA on H2O2 production was detected in the colorimetric assay. These results suggest that the fluorimetric assay with HVA is unsuitable for determining H2O2 formation by isolated cells.
Von Rein
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Sektion Pharmazie, Martin-Luther Universität, Postrach, Halle, Germany.
Hirschelmann
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D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003124:Colorimetry; D005260:Female; D005470:Fluorometry; D006719:Homovanillic Acid / Q000032:analysis; D006861:Hydrogen Peroxide / Q000378:metabolism*; D066298:In Vitro Techniques; D008264:Macrophages / Q000737:chemistry* / Q000378:metabolism; D008751:Methylene Blue; D010994:Pleura / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D006861:Hydrogen Peroxide; D013755:Tetradecanoylphorbol Acetate; D008751:Methylene Blue; D006719:Homovanillic Acid