Inhibition of IL-8-induced W3/25+ (CD4+) T lymphocyte recruitment into subcutaneous tissues of rats by selective depletion of in vivo neutrophils with a monoclonal antibody.
IL-8 recruits both neutrophils and lymphocytes in vitro and in vivo. To elucidate the mechanisms of lymphocyte recruitment in vivo by IL-8, we examined the role of neutrophil infiltration through selective depletion of circulating neutrophils using a mAb, RP-3. Selective depletion of neutrophils inhibited the IL-8 induced in vivo migration of the W3/25+ (CD4+) T cell subset but did not inhibit that of the MRC-OX8+ (CD8+) subset. These results suggest that CD4+ T cell migration into IL-8-injected s.c. tissues depends on the prior infiltration of neutrophils chemoattracted directly by IL-8.
Department of Parasitology, Yamagata University School of Medicine, Japan.
C
C
Kudo
A
A
Araki
K
K
Matsushima
F
F
Sendo
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015704:CD4 Antigens / Q000032:analysis*; D002465:Cell Movement; D004305:Dose-Response Relationship, Drug; D016209:Interleukin-8 / Q000494:pharmacology*; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D051381:Rats; D012867:Skin / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology*
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D016209:Interleukin-8