Regulation of ion and water transport across the eel intestine: effects of acetylcholine and serotonin.
Both acetylcholine (ACh) and serotonin (5-HT) lowered the serosa-negative transepithelial potential difference (PD) and the short-circuit current (Isc), accompanied by a decrease in NaCl and water absorption across the eel intestine. These inhibitory effects of ACh and 5-HT were blocked by atropine, a muscarinic receptor antagonist, and ICS-205930, a 5-HT3 receptor antagonist, respectively. Even after blocking the ACh receptor with atropine, 5-HT inhibited the PD and Isc, and ACh lowered them after blocking the 5-HT receptor with ICS-205930, indicating that ACh and 5-HT act independently. Similar inhibition in the PD and the Isc was observed after electrical field stimulation (EFS) which is expected to release endogenous regulators. These effects of EFS were reduced by 70% after simultaneous addition of atropine and ICS-205930. Since atropine and ICS-205930 block ACh and 5-HT receptors, respectively, these results suggest that endogenous ACh and 5-HT are released by EFS.
Faculty of Integrated Arts and Sciences, Hiroshima University, Japan.
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Mori
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Ando
D000109:Acetylcholine / Q000494:pharmacology*; D000811:Anguilla; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D004558:Electric Stimulation; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D007211:Indoles / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects*; D007477:Ions; D008564:Membrane Potentials / Q000187:drug effects; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism; D011985:Receptors, Serotonin / Q000187:drug effects / Q000378:metabolism; D012701:Serotonin / Q000494:pharmacology*; D012702:Serotonin Antagonists / Q000494:pharmacology; D000077526:Tropisetron; D014867:Water / Q000378:metabolism
D007211:Indoles; D007477:Ions; D011976:Receptors, Muscarinic; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; D014867:Water; D012701:Serotonin; D000077526:Tropisetron; D001285:Atropine; D000109:Acetylcholine