Application of the neutral red assay (NR assay) to monolayer cultures of primary hepatocytes: rapid colorimetric viability determination for the unscheduled DNA synthesis test (UDS).
The neutral red (NR) absorption method was adapted for the determination of cell viability in the UDS assay with primary hepatocyte cultures of the rat. The NR method is rapid, easy to perform, and suitable for handling of large numbers of cultures simultaneously. It can be used for concentration range-finding pre-experiments. In addition, it can easily be integrated into a UDS test protocol for documentation of toxic effects if supplementary cultures for each concentration are established. The time schedule required for the NR assay makes it possible for one person to process the hepatocytes for autoradiography and at the same time determine the toxicity.
Cytotest Cell Research GmbH & Co. KG (CCR), Rossdorf, F.R.G.
R
R
Fautz
B
B
Husein
C
C
Hechenberger
D015073:2-Acetylaminofluorene / Q000633:toxicity; D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D004121:Dimethyl Sulfoxide; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008297:Male; D008741:Methyl Methanesulfonate / Q000633:toxicity; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009499:Neutral Red; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry
D009153:Mutagens; D009499:Neutral Red; D015127:9,10-Dimethyl-1,2-benzanthracene; D015073:2-Acetylaminofluorene; D008741:Methyl Methanesulfonate; D004121:Dimethyl Sulfoxide