Direct radioimmunoassay of androstenediol-3-sulfate in the serum of normal men.
A commercially available antidihydrotestosterone antiserum was used for the direct radioimmunoassay of androstenediol-3-sulfate (ADS) in human serum. Aliquots of 1 or 2 microliter male serum (mean age of 40 subjects, 38.2 +/- 5.0 years) were diluted and extracted with ethanol for assay. The tracer, [7-3H]ADS, was prepared by sodium borohydride reduction of [7-3H]dehydroepiandrosterone sulfate (DS). Significantly cross-reacting steroids were testosterone, DS, androsterone sulfate, and epiandrosterone sulfate, which combined to produce a mean overestimation of ADS of 4.3 micrograms/dl in male serum. Mean serum ADS was 23.6 +/- 10.0 micrograms/dl (SD) in 20 fresh-frozen sera versus 28.4 +/- 9.7 micrograms/dl (SD) in 20 long-term (24.4 +/- 1.2 years) frozen specimens, showing stability on long-term frozen storage. Androstenediol-3-sulfate also showed a strong correlation with serum DS (r = 0.75). The possible physiologic significance of ADS is discussed, particularly in terms of the known estrogenicity of unconjugated androstenediol.
Orentreich Foundation for the Advancement of Science, Inc., Cold Spring-on-Hudson, NY 10516.
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D000328:Adult; D015114:Androstenediol / Q000031:analogs & derivatives* / Q000097:blood; D000918:Antibody Specificity; D004355:Drug Stability; D005189:False Positive Reactions; D005615:Freezing; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008297:Male; D011786:Quality Control; D011863:Radioimmunoassay / Q000379:methods*; D012016:Reference Values
D007106:Immune Sera; C033537:5-androstene-3 beta,17 beta-diol sulfate; D015114:Androstenediol