Characterization of Mycobacterium species by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
A rapid method has been standardized for extraction and SDS-PAGE analyses of mycobacterial cellular protein. Complex patterns were reproducibly obtained of the 31 reference strains or clinical isolates studied, belonging to 11 species. These patterns were species specific; thus this method could be an easy tool for mycobacterial strain characterization.
Centre d'Immunochimie Microbienne, Institut Pasteur, Lentilly, France.
A
A
Fevre
J
J
Fougerat
S
S
Bruneau
R
R
Guinet
D001426:Bacterial Proteins / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005069:Evaluation Studies as Topic; D009161:Mycobacterium / Q000737:chemistry / Q000145:classification*; D012967:Sodium Dodecyl Sulfate; D013045:Species Specificity
D001426:Bacterial Proteins; D012967:Sodium Dodecyl Sulfate