Random decarboxylation of uroporphyrinogen III by human hepatic uroporphyrinogen decarboxylase.
The type III heptacarboxylic porphyrinogens derived from enzymic decarboxylation of an acetic acid substituent on uroporphyrinogen III to a methyl group by human hepatic uroporphyrinogen decarboxylase has been analysed by reversed-phase high-performance liquid chromatography with electrochemical detection. The results showed that all four possible heptacarboxylic acid porphyrinogen isomers, with the methyl group attached to rings A, B, C and D of the tetrapyrrole macrocycle, respectively, were formed in almost equal proportions. It was concluded that the normal pathway of uroporphyrinogen III decarboxylation in human liver follows a random mechanism.
Luo
J L
JL
Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex, U.K.
Lim
C K
CK
D002851:Chromatography, High Pressure Liquid; D003653:Decarboxylation; D006801:Humans; D007536:Isomerism; D008099:Liver / Q000201:enzymology*; D011164:Porphyrias / Q000201:enzymology / Q000378:metabolism; D012871:Skin Diseases / Q000201:enzymology / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology; D014575:Uroporphyrinogen Decarboxylase / Q000378:metabolism*; D014577:Uroporphyrinogens / Q000378:metabolism*
D014577:Uroporphyrinogens; D014575:Uroporphyrinogen Decarboxylase