Crystallization and preliminary X-ray investigation of a sarcoplasmic calcium-binding protein from amphioxus.
Crystals of a sarcoplasmic Ca(2+)-binding protein from the protochordate amphioxus have been grown from solutions of ammonium sulfate. The crystals are orthorhombic, space group C222(1), with unit cell axes a = 59.6(1) A, b = 81.3(1) A and c = 82.4(1) A. There is one molecule in the asymmetric unit. The crystals diffract beyond 2.5 A and show less than 20% decline in diffraction intensities after a three day exposure to X-rays from a laboratory rotating anode source.
Department of Pathology, University of Alabama, Birmingham 35294.
W J
WJ
Cook
Y S
YS
Babu
J A
JA
Cox
D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D002135:Calcium-Binding Proteins / Q000737:chemistry*; D002816:Chordata, Nonvertebrate / Q000737:chemistry*; D003460:Crystallization; D003461:Crystallography; D012519:Sarcoplasmic Reticulum / Q000737:chemistry*; D014961:X-Ray Diffraction
D002135:Calcium-Binding Proteins; D000645:Ammonium Sulfate