Two different Escherichia coli capsular polysaccharide depolymerases each associated with one of the coliphage phi K5 and phi K20.
The Escherichia coli capsular polysaccharides (K antigens) K5 and K20 are known as primary receptors for the coliphage phi K5 and phi K20, respectively. A host range study of the phage revealed that E. coli K5 strains were not only lysed by phi K5 but also by phi K20, and furthermore that the E. coli K95 test strain was attacked by phi K5 in addition to K5 strains. In order to find out whether the phage can degrade the K antigens, the interaction of the phage with isolated polysaccharides was studied. It could be demonstrated that phi K5 was able to depolymerize the K5 and K95 polysaccharides and that phi K20 showed degrading activity towards the antigens K20 and K5. Obviously, each of the phages was associated with two different enzyme systems which enabled them to recognize and depolymerize chemically unrelated polysaccharides.
Institut für Medizinische Mikrobiologie, Medizinische Fakultät, Universität Rostock, F.R.G.
W
W
Nimmich
G
G
Schmidt
U
U
Krallmann-Wenzel
D000117:Acetylglucosamine / Q000032:analysis; D000118:Acetylglucosaminidase / Q000378:metabolism; D000942:Antigens, Bacterial / Q000378:metabolism*; D000954:Antigens, Surface / Q000378:metabolism*; D002240:Carbohydrate Sequence; D003090:Coliphages / Q000201:enzymology* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D013400:Sugar Acids / Q000032:analysis
D000942:Antigens, Bacterial; D000954:Antigens, Surface; C030659:K antigens; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate; D006026:Glycoside Hydrolases; C020668:glycanase; D000118:Acetylglucosaminidase; D000117:Acetylglucosamine