Analysis of cerebrospinal fluid from patients with psychiatric and neurological disorders by two-dimensional electrophoresis: identification of disease-associated polypeptides as fibrin fragments.
Two-dimensional electrophoresis (2-DE) of cerebrospinal fluid (CSF) samples--from 347 patients with various psychiatric and neurological disorders--and subsequent silver staining revealed two additional polypeptides (Mr 40,000) in 49% of 111 schizophrenics, 46% of 43 schizoaffective patients, 36% of 41 patients with affective disorders, 43% of 28 patients with multiple sclerosis, but not in 25 patients without neurological symptomatology, nor in 9 patients with Lues, and in only 2 of 25 patients with AIDS. The two polypeptides, as detected by 2-DE, eluted after size exclusion chromatography in fractions containing proteins with Mr greater than 200,000. After 2-DE of CSF samples, enriched by gel chromatography, the polypeptides were immobilized by blotting onto glass-fiber membranes and subjected to N-terminal sequencing. Polypeptide A was identified as beta-chain remnant (beta 2), derived from plasmin cleavage of fibrin(ogen). After size exclusion chromatography, 2-DE, and Western blotting, polypeptide A and B, as well as several other spots, reacted with fibrinogen antibodies, suggesting that the polypeptides are subunits of a fibrin degradation complex.
Psychiatric Hospital, University of Munich, Germany.
D B
DB
Wildenauer
D
D
Körschenhausen
W
W
Hoechtlen
M
M
Ackenheil
M
M
Kehl
F
F
Lottspeich
D000163:Acquired Immunodeficiency Syndrome / Q000134:cerebrospinal fluid; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D002850:Chromatography, Gel; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005338:Fibrin Fibrinogen Degradation Products / Q000134:cerebrospinal fluid*; D006801:Humans; D015151:Immunoblotting; D001523:Mental Disorders / Q000134:cerebrospinal fluid*; D008970:Molecular Weight; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid*; D011618:Psychotic Disorders / Q000134:cerebrospinal fluid; D012559:Schizophrenia / Q000134:cerebrospinal fluid; D012834:Silver
D002556:Cerebrospinal Fluid Proteins; D005338:Fibrin Fibrinogen Degradation Products; D012834:Silver