Coffee contains cholinomimetic compound distinct from caffeine. I: Purification and chromatographic analysis.
Both regular and decaffeinated coffees were found to have cholinomimetic actions when tested in urethane-anesthetized rats. These actions were distinct from those of caffeine and reversible by atropine. The bioactive fraction was purified from alcoholic extracts of instant decaffeinated coffee by liquid column chromatography and preparative TLC. The purified compound showed similar pharmacological actions as the starting material. Chromatographic behavior was further characterized by analytical TLC and HPLC. Chromatographic analyses of extracts of green coffee beans and roasted ground coffees showed that the cardioactive compound was only present in roasted coffees. Similar analyses of other commonly consumed beverages, including teas and cocoa, showed that this compound was not present in beverages besides coffee.
Department of Neurology, Georgetown University, Washington, D.C. 20007.
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D000109:Acetylcholine / Q000032:analysis; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects; D002099:Cacao / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003069:Coffee / Q000032:analysis*; D008297:Male; D010277:Parasympathomimetics / Q000032:analysis / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet; D013662:Tea / Q000032:analysis
D003069:Coffee; D010277:Parasympathomimetics; D013662:Tea; D001285:Atropine; D000109:Acetylcholine