Analysis of the aneuploidy inducing capacity of 2-chlorobenzylidene malonitrile (CS) and metabolites in V79 Chinese hamster cells.
The aneuploidy inducing capacity of 2-chlorobenzylidene malonitrile (CS), a chemical used as a riot control agent, and its hydrolysis products o-chlorobenzaldehyde and malonitrile was studied at various exposure conditions in V79 Chinese hamster cells. Chromosomes were counted in metaphase preparations with preserved cytoplasm. A significant increase in the number of aneuploid cells was found after CS and o-chlorobenzaldehyde exposure but not after malonitrile exposure. o-chlorobenzaldehyde, which also introduced high levels of polyploid cells, seems to be an important metabolite for the aneuploidogenic effectiveness of CS.
Schmid
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Institut für Strahlenbiologie, GSF-Forschungszentrum für Umwelt und Gesundheit, Neuherberg-München, FRG.
Bauchinger
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M
D000782:Aneuploidy; D000818:Animals; D001547:Benzaldehydes / Q000633:toxicity*; D002460:Cell Line; D002875:Chromosomes / Q000187:drug effects; D004261:DNA Replication; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D015162:Micronucleus Tests; D009570:Nitriles / Q000633:toxicity*; D009763:o-Chlorobenzylidenemalonitrile / Q000378:metabolism / Q000633:toxicity*
D001547:Benzaldehydes; D009570:Nitriles; D009763:o-Chlorobenzylidenemalonitrile; C059341:2-chlorobenzaldehyde; C000945:dicyanmethane; D004121:Dimethyl Sulfoxide