Basic mechanisms of cellular priming and release of inflammatory mediators.
Simultaneous stimulation of human neutrophils (PMN) with the receptor-mediated activator formyl-met-leu-phe (FMLP) and the G protein activator sodium fluoride (NaF) resulted in the synergistic generation of leukotrienes. Activation of human platelets with thrombin and NaF showed an additive formation of 12-HETE. This enhancement in lipid mediator generation correlated with a time-dependent synergism in G protein activation after sequential stimulation of intact cells with FMLP and NaF or thrombin and NaF, respectively. In addition, polymerization of actin, an early event in cell activation, was enhanced after incubation with cytokines and FMLP.
Lehrstuhl für Medizinische Mikrobiologie und Immunologie, AG Infektabwehr, Ruhr-Universität Bochum, FRG.
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D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D002478:Cells, Cultured; D019204:GTP-Binding Proteins / Q000502:physiology; D006165:Guanylyl Imidodiphosphate / Q000378:metabolism; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis*; D015289:Leukotrienes / Q000096:biosynthesis*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D012969:Sodium Fluoride / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology
D006893:Hydroxyeicosatetraenoic Acids; D015289:Leukotrienes; D006165:Guanylyl Imidodiphosphate; D009240:N-Formylmethionine Leucyl-Phenylalanine; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D012969:Sodium Fluoride; D013917:Thrombin; D019204:GTP-Binding Proteins