Neurotensin-like immunoreactivity in presumptive baroreceptor neurons innervating the guinea pig carotid sinus.
This study was carried out to determine the ultrastructure and origin of previously described neurotensin-like immunoreactive (NT-LI) nerve fibres in the wall of the carotid sinus of the guinea pig. In our degeneration experiments, these NT-LI axons were unaffected by surgical sympathectomy but disappeared in response to transection of the carotid sinus nerve, thus suggesting a sensory origin. This assumption could be supported by combined retrograde tracing and immunohistochemistry. Primary afferent neurons of the sensory glossopharyngeal (petrosal) ganglion projecting to the carotid sinus were identified by injecting the retrograde fluorescent tracer, Fluoro-Gold, into the sinus wall, and some of these neurons displayed NT-LI. Within the carotid sinus, the terminals of these NT-LI sensory neurons exhibited ultrastructural features characteristic of baroreceptor endings, i.e. axonal swellings filled with mitochondria and closely associated to elastin. However, many endings also fulfilling the ultrastructural criteria for baroreceptors were devoid of immunolabelling. Thus, we conclude that the NT-LI terminals constitute a subgroup rather than the entire population of baroreceptor endings within the guinea pig carotid sinus. With respect to the established pharmacological effects of NT in guinea pig, we propose an involvement of NT-LI fibres in the modulation of baroreception at the peripheral level.
Institute for Anatomy and Cell Biology, University of Heidelberg, F.R.G.
W
W
Kummer
M
M
Reinecke
C
C
Heym
D000818:Animals; D002346:Carotid Sinus / Q000294:innervation* / Q000648:ultrastructure; D005260:Female; D005456:Fluorescent Dyes; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009474:Neurons / Q000378:metabolism*; D009496:Neurotensin / Q000378:metabolism*; D011311:Pressoreceptors / Q000378:metabolism*; D013266:Stilbamidines; D013562:Sympathectomy; D014018:Tissue Distribution
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D013266:Stilbamidines; D009496:Neurotensin