Human T-lymphotropic virus type I genome in adult T-cell leukemia/lymphoma.
The proviral DNA of human T-lymphotropic virus type I (HTLV-I) was examined in 8 patients with adult T-cell leukemia/lymphoma (ATL/L) by Southern blot analysis. Judging from the number of bands containing viral DNA sequences in tumor cells, one and three copies of proviral genome were detected in 7 and 1 cases, respectively. The proviral genome in 1 case contained variant PstI sites and a single defective provirus which lost 5' LTR, gag and a part of pol genes was found in 2 cases. However, the pX region was detected in both of 2 latter cases by using the polymerase chain reaction. The results indicate that not all tumor cells from ATL/L yield infectious viruses and also support that the product of pX region may contribute to the tumorigenesis of HTLV-I.
Yang
Y C
YC
Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Republic of China.
Hsu
T Y
TY
Wang
C H
CH
Su
I J
IJ
Chen
J Y
JY
Yang
C S
CS
D000328:Adult; D000368:Aged; D015139:Blotting, Southern; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005814:Genes, Viral; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics; D016662:Virus Integration
D004279:DNA, Viral