Cultured hepatocytes bind and internalize bovine serum amine oxidase-gold complexes.
Cultured hepatocytes express binding sites for bovine serum amine oxidase on their membrane surfaces as evaluated at the electron microscopic level by using enzyme-gold complexes. Hepatocytes show binding sites as small clusters of gold granules, not bound in a specialized region of the plasma membrane. The binding competition of enzyme-gold ligand to cells was achieved by preincubation with uncoupled bovine serum amine oxidase. In addition, N-acetyl-galactosamine, N-acetyl-glucosamine and Mannose, at the final concentration of 80 mM, partially inhibit the binding.
Department of Biology, University of Rome, Tor Vergata, Italy.
L
L
Dini
E
E
Agostinelli
B
B
Mondoví
D006631:Amine Oxidase (Copper-Containing); D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002478:Cells, Cultured; D006046:Gold; D007700:Kinetics; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009005:Monosaccharides / Q000494:pharmacology; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000097:blood / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains
D009005:Monosaccharides; D006046:Gold; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors