Mutagenicity of processed bidi tobacco: possible relevance to bidi industry workers.
The genotoxic potential of bidi tobacco was evaluated by mutagenicity testing of aqueous, aqueous: ethanolic, ethanolic and chloroform extracts of processed tobacco used in the manufacture of 'bidis', indigenous forms of cigarettes smoked in India. The Salmonella/mammalian microsome test (Ames assay) was used to detect mutagenicity in tester strains TA98, TA100 and TA102. The extracts were tested in the absence and presence of metabolic activation using liver S9 from rat and hamster, and following in vitro nitrosation with sodium nitrite at acidic pH. All the extracts were non-mutagenic in the absence of nitrosation. The nitrosated aqueous extract was mutagenic in strains TA98 and TA100. While weak mutagenicity was elicited by the nitrosated aqueous: ethanolic extract in TA100, the nitrosated ethanolic extract induced a 3-fold increase in the number of revertants in the same strain. Moreover both these extracts elicited a strong mutagenic response in TA102, while the chloroform extract was non-mutagenic even after nitrite treatment. The present study indicates that workers employed in the bidi industry are exposed to potentially mutagenic and genotoxic chemicals in the course of their occupation.
Cancer Research Institute, Tata Memorial Centre, Parel, Bombay, India.
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Bagwe
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Bhisey
D001711:Biotransformation; D004305:Dose-Response Relationship, Drug; D009152:Mutagenicity Tests; D010947:Plants, Toxic; D012486:Salmonella typhimurium / Q000235:genetics; D012977:Sodium Nitrite / Q000494:pharmacology; D014030:Tobacco, Smokeless / Q000633:toxicity*
D012977:Sodium Nitrite