Anemia in new congenital adult type polycystic kidney mice.
Mechanisms for the development of anemia and the effects of recombinant human erythropoietin (r-HuEPO) on hematological parameters were studied in new congenital adult type polycystic kidney (DBA/2FG-pcy) mice. The majority of DBA/2FG-pcy mice showed progressive anemia and an elevation of blood urea nitrogen, while a minority showed progressive anemia following polycythemia. Kidneys with numerous cysts in the cortex and medulla occupied virtually the entire abdominal cavity, and the combined kidney weight taken as a percentage of body weight reached 13.5% in the DBA/2FG-pcy mouse. The osmotic fragility of DBA/2FG-pcy mice erythrocytes was significantly increased compared with that of normal control mice. In addition, two-fold increases in serum EPO levels, determined by radioimmunoassay, and a decreased number of colony forming unit-erythroid (CFU-E) were observed in the DBA/2FG-pcy mice. The administration of r-HuEPO during anemia significantly increased the red blood cell count, hemoglobin concentration, hematocrit and reticulocyte percentage in a dose-dependent manner. These findings indicate that anemia in the DBA/2FG-pcy mouse is due to increased fragility of erythrocytes, a deficiency in EPO for the degree of anemia and a decreased number or a decreased response of erythroid progenitor cells. We suggest that the DBA/2FG-pcy mouse is a useful spontaneous model of chronic progressive renal failure.
Pharmaceutical Laboratory, Kirin Brewery Co., Ltd., Gunma, Japan.
J
J
Koumegawa
N
N
Nagano
H
H
Arai
M
M
Wada
M
M
Kusaka
H
H
Takahashi
D000740:Anemia / Q000097:blood / Q000209:etiology* / Q000628:therapy; D000818:Animals; D001806:Blood Urea Nitrogen; D001853:Bone Marrow / Q000473:pathology; D003114:Colony-Forming Units Assay; D004195:Disease Models, Animal; D004906:Erythrocyte Count; D004921:Erythropoietin / Q000097:blood / Q000627:therapeutic use*; D006400:Hematocrit; D007668:Kidney / Q000473:pathology; D051379:Mice; D009996:Osmotic Fragility; D007690:Polycystic Kidney Diseases / Q000097:blood / Q000150:complications* / Q000473:pathology; D011994:Recombinant Proteins
D011994:Recombinant Proteins; D004921:Erythropoietin