A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA.
BALB/c, (BALB/c x B10.A)F1 and (BALB/c x B10)F1 hybrid mice were immunized with C-peptide of human proinsulin. The (BALB/c x B10.A)F1 hybrids were the best responders and yielded 3 hybridomas secreting specific monoclonal antibodies. One of them, C-PEP-01, bound the C-peptide with high affinity (Kas = 1.1 x 10(9) l/mol), cross-reacted fully with human proinsulin but not with insulin, glucagon or somatostatin and apparently recognized the regions of C-peptide comprising amino acid residues 8-13 and 25-31. A RIA system could be set up employing this monoclonal antibody suitable for estimation of C-peptide concentrations in a diagnostically useful range (1-50 ng/ml).
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
I
I
Hilgert
P
P
Stolba
H
H
Kristofová
I
I
Stefanová
B
B
Bendlová
M
M
Lebl
V
V
Horejsí
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002096:C-Peptide / Q000032:analysis / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D002096:C-Peptide; D010446:Peptide Fragments