High-performance liquid chromatographic determination, with ultraviolet detection, of S-adenosyl-L-methionine and S-adenosyl-L-homocysteine in rat tissues and simultaneously of normetanephrine and metanephrine for phenylethanolamine-N-methyltransferase or catechol-O-methyltransferase activities.
A high-performance liquid chromatographic method, with ultraviolet detection, for the determination of S-adenosyl-L-methionine and S-adenosyl-L-homocysteine, and simultaneously of normetanephrine and metanephrine, is presented. The separation was carried out by reversed-phase ion-pair isocratic chromatography. This procedure was applied to the study of the content of S-adenosyl-L-methionine and S-adenosyl-L-homocysteine in rat brain and adrenal glands and to the measurement of phenylethanolamine-N-methyltransferase or catechol-O-methyltransferase activities in rat adrenal gland.
Laboratoire d'Eutonologie, Hôpital Boucicaut, Paris, France.
B
B
Guattari
D000311:Adrenal Glands / Q000737:chemistry; D000818:Animals; D001923:Brain Chemistry; D002394:Catechol O-Methyltransferase / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D008297:Male; D008676:Metanephrine / Q000378:metabolism*; D009647:Normetanephrine / Q000378:metabolism*; D010625:Phenylethanolamine N-Methyltransferase / Q000378:metabolism*; D051381:Rats; D012435:S-Adenosylhomocysteine / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D009647:Normetanephrine; D008676:Metanephrine; D012436:S-Adenosylmethionine; D012435:S-Adenosylhomocysteine; D010625:Phenylethanolamine N-Methyltransferase; D002394:Catechol O-Methyltransferase