Temporary changes of protein level in liver after AET or MEA treatment prior to 60Co gamma-irradiation of male mice.
The adult male Swiss mice were either whole-body gamma-irradiated with a single dose of 10 Gy from 60Co source, always at 19.00 or, 15 minutes before irradiation injected intraperitoneally with AET (2-aminoethylisothiouronium Br. HBr), or MEA (cysteamine HCl), in a dose of 400 mg/kg body weight. The measurements of the protein level in crude homogenates of liver were done in four-hour internals during a 24-hour period, starting at 20.00. The protein concentration in liver was calculated per 1 g of fresh tissue and the whole organ weight. The body and liver weight was also studied. There were no fluctuations in the liver weight and concentration of protein in the control and irradiated only mice. Temporary changes in the liver weight and level of protein expressed in mg per 1 g of fresh tissue and the whole organ weight could be found in the group of males treated with AET, and daily changes in the liver weight and concentration of protein related to mg per 1 g of fresh tissue, in the group of male mice injected with MEA prior to irradiation, could be recorded. Differences in the liver weight at 20.00, 24.00 and 04.00, as well as in the protein level expressed in mg per 1 g fresh tissue at 04.00, 12.00, 16.00, and the whole liver weight at 24.00, 04.00, and 16.00, of between the particular groups of mice, were observed. There were no temporary changes in the body weight in any of the groups and there were no differences in this value between the groups of mice.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Animal Physiology, Jagiellonian University, Cracow, Poland.
L
L
Mazur
J
J
Manowska
D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D003037:Cobalt Radioisotopes; D003543:Cysteamine / Q000494:pharmacology*; D005720:Gamma Rays; D008099:Liver / Q000737:chemistry* / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects / Q000502:physiology; D011506:Proteins / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D014916:Whole-Body Irradiation; D000338:beta-Aminoethyl Isothiourea / Q000494:pharmacology*
D003037:Cobalt Radioisotopes; D011506:Proteins; D000338:beta-Aminoethyl Isothiourea; D003543:Cysteamine