Construction of a Saccharomyces cerevisiae strain able to ferment cellobiose.
The bglA gene, encoding a beta-glucosidase from Bacillus polymyxa, has been expressed in Saccharomyces cerevisiae under control of the CYC-GAL promoter inducible by galactose. The expression of bglA-encoded activity in the strain used as a host was not sufficient to allow its growth with cellobiose as a carbon source. However, a recessive mutation in a gene designated cem1 has been obtained which, combined with the expression of beta-glucosidase activity, allows the growth of S. cerevisiae on cellobiose. The expression of the blgA gene in a cem1 strain confers on S. cerevisiae the capability for an efficient fermentation of cellobiose, as detected by the formation of CO2.
Instituto de Agroquímica y Tecnología de Alimentos, Valencia, Spain.
A C
AC
Adam
J
J
Polaina
D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D002475:Cellobiose / Q000378:metabolism*; D003001:Cloning, Molecular; D005285:Fermentation / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D009154:Mutation; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D001617:beta-Glucosidase / Q000235:genetics*
D002475:Cellobiose; D001617:beta-Glucosidase