The gene for the alpha 4 subunit of the VLA-4 integrin maps to chromosome 2Q31-32.
The VLA-4 integrin (CD49d/CD29), initially discovered on lymphoid cells, is actually known to be highly expressed on T cells, B cells, monocytes, and derived cell lines. Unlike other VLA integrins, mainly involved in cell-matrix adhesive interactions, VLA-4 has also been implicated in several cellular interactions. Based on the published alpha 4 cDNA sequence, a 1,142-bp alpha 4 cDNA fragment was amplified using the polymerase chain reaction. This fragment was used to isolate three overlapping genomic clones from a phage library. By Southern analysis with the cDNA probe, and using the polymerase chain reaction on DNA isolated from a panel of human/mouse somatic cell hybrids, the alpha 4 gene was mapped to chromosome 2. Fluorescence in situ hybridization confirmed this assignment and allowed a more precise mapping to chromosome 2q31-32.
Center for Human Genetics, University of Leuven, Belgium.
Z H
ZH
Zhang
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Vekemans
M S
MS
Aly
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M
Jaspers
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Marynen
J J
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Cassiman
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D015246:Deoxyribonuclease EcoRI; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016029:Receptors, Very Late Antigen / Q000235:genetics*
D005456:Fluorescent Dyes; D016029:Receptors, Very Late Antigen; D004247:DNA; D015246:Deoxyribonuclease EcoRI; D016650:Fluorescein-5-isothiocyanate