The gene DIS2S1 is essential in Saccharomyces cerevisiae and is involved in glycogen phosphorylase activation.
S. cerevisiae gene DIS2S1, which codes for a protein very similar to the catalytic subunit of mammalian protein phosphatase 1, was disrupted "in vitro". Diploid yeast cells were transformed and sporulated. Tetrad analysis demonstrated that disruption of DIS2S1 is lethal for the cell. Glycogen phosphorylase alpha and glycogen synthase activity ratio were measured in diploids carrying a disrupted allele of the gene. Phosphorylase was dramatically activated in mutant cells but, under the same conditions, glycogen synthase activity was essentially identical in both mutant and wild-type cells.
Department de Bioquímica i Biología Molecular, Facultat de Veterinaria, Universitat Autònoma de Barcelona, Spain.
J
J
Clotet
F
F
Posas
A
A
Casamayor
I
I
Schaaff-Gerstenschläger
J
J
Ariño
D002384:Catalysis; D004171:Diploidy; D004789:Enzyme Activation; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism; D006006:Glycogen Synthase / Q000378:metabolism; D009154:Mutation; D006005:Phosphorylases / Q000378:metabolism*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D006005:Phosphorylases; D006006:Glycogen Synthase; D005947:Glucose