Selective impairment of T lymphocyte activation following contact sensitization with oxazolone.
We have previously reported that topical exposure of mice to oxazolone results in the appearance of regulatory mechanisms which markedly depress lymph node cell (LNC) proliferative responses to subsequent challenge with the same chemical. In the present study, we have sought to identify the cellular targets for such immunoregulation. Autoradiographic analyses revealed that although pre-exposure to oxazolone caused a substantial reduction of paracortical hyperplasia following challenge, the frequency of proliferating cells in lymphoid follicles was slightly increased. That B lymphocyte responses are unaffected by oxazolone-induced immunoregulation was confirmed by investigation of anti-hapten antibody formation by draining LNC. Challenge with oxazolone resulted in an accelerated antibody response in mice previously exposed to the same chemical. These data reveal that the active immunoregulation induced following sensitization with oxazolone is selective for T lymphocytes. Evidence is presented that CD4+ and CD8+ T lymphocytes possess equivalent sensitivity to these mechanisms.
ICI Central Toxicology Laboratory, Alderley Park, Macclesfield, UK.
I
I
Kimber
J R
JR
Foster
D
D
Baker
J L
JL
Turk
D000287:Administration, Topical; D000818:Animals; D000937:Antigen-Antibody Reactions; D001402:B-Lymphocytes / Q000187:drug effects; D015704:CD4 Antigens / Q000096:biosynthesis; D016827:CD8 Antigens / Q000096:biosynthesis; D015536:Down-Regulation / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007156:Immunologic Memory; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D010081:Oxazolone / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects*; D013997:Time Factors
D015704:CD4 Antigens; D016827:CD8 Antigens; D010081:Oxazolone