Identification of human esterase D subunits from the homodimeric and heterodimeric forms of five phenotypes by a new two-dimensional isoelectric focusing method.
A new two-dimensional isoelectric focusing method was developed to identify the human esterase D (EsD) subunits from the homodimeric and heterodimeric forms of five EsD phenotypes. EsD polymorphism was also analyzed by one-dimensional isoelectric focusing under reducing and mild denaturing conditions to study the influence of dithiothreitol and low concentrations of urea on the focusing pattern of the EsD dimers.
Seccion de Biologia, Instituto Nacional de Toxicologia, Madrid, Spain.
A
A
Alonso
G
G
Visedo
M
M
Sancho
J
J
Fernández-Piqueras
D000483:Alleles; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000097:blood* / Q000235:genetics; D002882:Chromosomes, Human, Pair 13; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology; D005796:Genes; D005819:Genetic Markers; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D007527:Isoenzymes / Q000097:blood* / Q000235:genetics; D010084:Oxidation-Reduction; D010641:Phenotype; D011489:Protein Denaturation
D005819:Genetic Markers; D007527:Isoenzymes; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C020118:ESD protein, human