Genetic control of the immune response to Trichinella spiralis: recognition of muscle larval antigens.
Host antibody recognition of muscle larval (ML) antigens of Trichinella spiralis was examined. Monoclonal antibodies (MoAbs) to known host protective ML antigens have been produced in order to aid this examination. Eleven strains of mice with independent MHC haplotypes and seventeen T. spiralis infected human patients were all found to recognize the same three major antigens as the monoclonal antibodies; i.e., of mw 41, 46 and 55 kD. However all serum samples tested also recognized further ML antigens and this recognition varied with the individual or strain. This variation in antigen recognition also applied to the MoAb. Mutual inhibition studies demonstrated that even where the MoAb apparently recognized the same antigens, specific epitope recognition was disparate. Hence some of the major antigens recognized by hosts of T. spiralis, regardless of whether vaccinated or infected, correspond with antigens which have considerable host protective properties. There also appear to be a number of epitopes upon these antigens and the biological implications of this are discussed.
Department of Immunology, Mayo Clinic, Rochester, MN 55905.
M
M
Robinson
C J
CJ
Krco
T G
TG
Beito
C S
CS
David
D000818:Animals; D000909:Antibodies, Helminth / Q000235:genetics / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology*; D001667:Binding, Competitive / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007125:Immunogenetics; D007814:Larva / Q000276:immunology; D051379:Mice; D008817:Mice, Mutant Strains; D008970:Molecular Weight; D009124:Muscle Proteins / Q000276:immunology*; D011863:Radioimmunoassay; D014234:Trichinella / Q000235:genetics / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology* / Q000469:parasitology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D009124:Muscle Proteins