A B cell population that dominates the primary response to influenza virus hemagglutinin does not participate in the memory response.
The early primary B cell response of BALB/c mice to the influenza virus A/PR/8/34 (PR8) hemagglutinin (HA) is dominated by B cells that utilize a single V kappa gene in association with one of two closely related VH genes. We have used an anti-idiotypic reagent that recognizes a light chain-associated idiotope (23-1 Id) on these antibodies to follow their presence during the anti-HA response. Quantitation of 23-1Id+ antibodies at different time points during the anti-HA response indicates that the 23-1Id+ B cell response peaks early after primary immunization and is not re-induced by secondary challenge. Furthermore, 23-1Id+ titers in serum decay rapidly between the first and second week after immunization, and the HA-specific 23-1Id+ precursor B cell population does not significantly expand in the months following immunization. These results indicate that despite their predominance during the primary response, 23-Id+ B cells abruptly disappear from the response and do not mature into memory B cells.
Kavaler
J
J
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Caton
A J
AJ
Staudt
L M
LM
Gerhard
W
W
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D000916:Antibody Diversity; D000956:Antigens, Viral / Q000276:immunology; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D006389:Hemagglutinins, Viral / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D007132:Immunoglobulin Isotypes / Q000276:immunology; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D007156:Immunologic Memory; D009980:Influenza A virus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D006389:Hemagglutinins, Viral; D007130:Immunoglobulin Idiotypes; D007132:Immunoglobulin Isotypes; D007145:Immunoglobulin kappa-Chains