[Lysosomal activity in enterocytes in the small intestine in piglets experimentally infected with Isospora suis].
The lysosomal activity of enterocytes of the small intestine mucosa was investigated in gnotobiotic and conventional piglets experimentally infected on the first day after birth (DAB) by the oocysts of the coccidia Isospora suis. A method of the proof of beta-D-glucuronidase (EC.3.2.1.31.) activity was used to demonstrate lysosomes. The piglets were infected by different infection doses of oocysts (100,000 oocysts in gnotobiotic piglets and 200,000 oocysts in conventional piglets). In the gnotobiotic infected piglets the activity of beta-D-glucuronidase in enterocyte lysosomes was investigated in the period from day 3 to day 11 after infection (DAI) and in the infected conventional piglets in the period from day 2 to day 10 after infection. Comparing the control piglets, the group of gnotobiotic piglets at the age of 2-5 days and the group of conventional piglets at the age of 4-7 days, the higher activity of beta-D-glucuronidase was demonstrated in the lysosomes of intestinal mucosa enterocytes in the gnotobiotic control piglets (+5.30 of the average density value, Dx). In the infected gnotobiotic and conventional piglets the pattern of beta-D-glucuronidase activity was found to have three stages in the course of this infection. Two stages can be characterized by a great increase in the enzyme activity (DAI 3-9 in gnotobiotic piglets, DAI 2-3 and 7-9 in conventional piglets. The third stage, which is manifest mainly in the conventional infected piglets, is characterized by a marked decrease in the activity of beta-D-glucuronidase, reaching the level of control findings (DAI 10 and mainly 11 in gnotobiotic piglets. DAI 4-6 and 10 in conventional piglets). A topographical picture shows that the two stages of increase and the stage of beta-D-glucuronidase activity decrease occur in the whole small intestine without any predisposition defect of the enzyme in the different sections of the small intestine.
Výzkumná a diagnostická histochemická laborator, Ceský Krumlov.
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Kudweis
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Lojda
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Julis
D000818:Animals; D003048:Coccidiosis / Q000201:enzymology*; D005966:Glucuronidase / Q000032:analysis*; D006651:Histocytochemistry; D007421:Intestine, Small / Q000201:enzymology* / Q000648:ultrastructure; D007549:Isospora; D008247:Lysosomes / Q000201:enzymology*; D013552:Swine; D013553:Swine Diseases / Q000201:enzymology*
D005966:Glucuronidase
Lysozomální aktivita enterocytů tenkého streva selat experimentálnĕ infikovaných kokcidií Isospora suis.