A pseudogene on the X chromosome for the human trifunctional enzyme MTHFD (methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase).
The human trifunctional folate-dependent protein MTHFD has been mapped to chromosome 14q24 and to the X chromosome was identified by screening an X-chromosome-specific library. Amplification by the polymerase chain reaction (PCR) and sequencing of PCR products indicate that the sequence is an intronless pseudogene.
Department of Pediatrics, McGill University, Montreal Children's Hospital Research Institute, Montreal, Quebec, Canada.
C
C
Italiano
S W
SW
John
D W
DW
Hum
R E
RE
MacKenzie
R
R
Rozen
D000619:Aminohydrolases / Q000235:genetics*; D001483:Base Sequence; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005574:Formate-Tetrahydrofolate Ligase / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP) / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011544:Pseudogenes; D014960:X Chromosome
D009097:Multienzyme Complexes; C055342:methylene tetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase; C021836:formyl-methenyl-methylenetetrahydrofolate synthetase; D004247:DNA; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP); D000619:Aminohydrolases; D005574:Formate-Tetrahydrofolate Ligase