Molecular diversity of SIVsmm/PBj and a cognate variant, SIVsmm/PGg.
The molecular diversity of SIVsmm/PBj proviral genomes in tissues of an infected macaque was analyzed. Molecular clones derived directly from intestinal tissue DNA were heterogenous, and contained LTRs with one or two NF-kB binding sites. LTRs with one NF-kB site predominated (approximately 75%). Virions derived from biologically active chimeric DNA clones with one or two NF-kB sites did not induce the acute death syndrome characteristic of PBj infection. These results suggest that either the duplicated NF-kB site acts in concert with other important viral determinants, or plays no role in producing the PBj syndrome.
Department of Microbiology, Georgetown University, Rockville, MD.
F J
FJ
Novembre
V M
VM
Hirsch
H M
HM
McClure
P R
PR
Johnson
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry*; D014644:Genetic Variation; D016322:HIV Enhancer; D015699:HIV Envelope Protein gp120 / Q000737:chemistry; D006801:Humans; D008254:Macaca nemestrina; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D010581:Peyer's Patches / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D016097:Simian Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics*; D013154:Spleen / Q000382:microbiology; D014759:Viral Envelope Proteins
D004279:DNA, Viral; D015699:HIV Envelope Protein gp120; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; C069623:gp120 protein, Simian immunodeficiency virus