Comparative evaluation of nine different methods for detecting enterotoxin of Escherichia coli.
Nine different methods for detecting enterotoxin of Escherichia coli were studied and compared. We found rabbit ileal-loop test and suckling mouse assay were both quite accurate and reliable for detecting heat labile toxin (LT) and heat stable toxin (ST) of enterotoxigenic Escherichia coli (ETEC). Mouse ileal-loop test was simple, but its sensitivity and specificity were comparatively low. CHO cell-culture assay might be more sensitive and specific. LT-DNA probe was the most sensitive and specific method. In practical application, PIHT (plate immunohemolytic test), Biken's, SPA-CoA and ELISA methods are recognized as simple, rapid, sensitive and specific methods for detecting ETEC-LT. These methods can be selected for use in clinical laboratory.
Zhu
Q Y
QY
Taiyuan Children's Hospital.
Li
L Q
LQ
Lin
W M
WM
Li
Y T
YT
Zhang
P Y
PY
Huang
Y T
YT
D000818:Animals; D001681:Biological Assay / Q000379:methods; D015342:DNA Probes; D004768:Enterotoxins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004926:Escherichia coli; D005779:Immunodiffusion; D051379:Mice; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D013205:Staphylococcal Protein A
D015342:DNA Probes; D004768:Enterotoxins; D013205:Staphylococcal Protein A