Neutrophil activating peptide-2 binds with two affinities to receptor(s) on human neutrophils.
Neutrophil receptor(s) for neutrophil activating peptides 1 and 2 were studied by competition binding experiments with radiolabeled NAP-1 and NAP-2 preparations. NAP-1 bound with one affinity, NAP-2 with two quite different affinities, to common receptor(s) on neutrophils. Concentrations of NAP-2 needed to induce exocytosis of beta-glucosaminidase corresponded to the higher dissociation constant of the two binding equilibria. Thus, the binding of NAP-2 to PMN with high affinity does not activate the cells.
Sandoz Forschungsinstitut, Vienna, Austria.
W
W
Schnitzel
B
B
Garbeis
U
U
Monschein
J
J
Besemer
D001667:Binding, Competitive; D004591:Electrophoresis, Polyacrylamide Gel; D005089:Exocytosis; D006596:Hexosaminidases / Q000378:metabolism; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D007700:Kinetics; D009504:Neutrophils / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism*; D023062:Receptors, Interleukin-8A; D011994:Recombinant Proteins / Q000378:metabolism
D016209:Interleukin-8; D011971:Receptors, Immunologic; D023062:Receptors, Interleukin-8A; D011994:Recombinant Proteins; D006596:Hexosaminidases