Linkage disequilibrium between two highly polymorphic microsatellites.
The PCR was used to amplify genomic DNA from two microsatellite (dC-dA)n.(dG-dT)n sequences found to be present in the same chromosome 5 genomic clone. Analysis of the haplotype frequencies of these two interspersed repeat sequences in individuals showed strong allelic association or linkage disequilibrium. Six alleles were found for p599 (CA)n with a PIC value of 0.71 and 8 alleles were seen for lambda 599 (CA)n with a PIC value of 0.74. The two microsatellites are separated by approximately 7 kb. Analysis of the length variations for the two microsatellites showed that they were positively correlated, a finding that has no obvious explanation. The strong linkage disequilibrium found demonstrates stability during evolution for these novel markers. Therefore they should be powerful new tools for studying genetic drift and admixture of populations. Furthermore, disequilibrium data from microsatellites can be used in the fine mapping and cloning of disease genes.
Academic Department of Psychiatry, University College and Middlesex School of Medicine, London, England.
R
R
Sherrington
G
G
Melmer
M
M
Dixon
D
D
Curtis
B
B
Mankoo
G
G
Kalsi
H
H
Gurling
D000483:Alleles; D001483:Base Sequence; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000235:genetics*; D003854:Deoxyribonucleotides; D005787:Gene Frequency; D015698:Genomic Library; D006801:Humans; D015810:Linkage Disequilibrium; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004276:DNA, Satellite; D003854:Deoxyribonucleotides