Conductance measurements in solid phase peptide synthesis. I. Monitoring coupling and deprotection in Fmoc chemistry.
Potentiated conductance shows promise for monitoring the progress of both coupling and deprotection reactions in solid phase peptide synthesis using Fmoc chemistry. Sterically hindered base may be added to the aprotic polar solvents used for synthesis, this induces ionisation of acids formed in the coupling and deprotection reactions, thus giving rise to a conductance signal in the solvent. Changes in this signal allow reactions occurring at the resin to be followed with no degradation of coupling efficiency.
McFerran
N V
NV
Division of Biochemistry, School of Biology & Biochemistry, Queen's University of Belfast, UK.
Walker
B
B
McGurk
C D
CD
Scott
F C
FC
D000215:Acylation; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D004563:Electrochemistry; D005449:Fluorenes / Q000737:chemistry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis*; D011487:Protein Conformation
D000596:Amino Acids; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D010455:Peptides