Cytokine- and forskolin-induced synthesis of group II phospholipase A2 and prostaglandin E2 in rat mesangial cells is prevented by dexamethasone.
We have previously described that treatment of rat glomerular mesangial cells with interleukin-1 beta, tumor necrosis factor or forskolin stimulates the synthesis and secretion of prostaglandin E2 and group II phospholipase A2. We now report that pretreatment of the mesangial cells with dexamethasone dose-dependently suppresses the cytokines- and forskolin-induced synthesis of prostaglandin E2 as well as the induced synthesis and secretion of group II phospholipase A2. These observations implicate that the inhibition of the cellular or secreted phospholipase A2 activity by dexamethasone in rat mesangial cells is not due to induced synthesis of phospholipase A2 inhibitory proteins but caused by direct inhibition of phospholipase A2 protein expression.
Centre for Biomembranes and Lipid Enzymology, Utrecht, The Netherlands.
C
C
Schalkwijk
M
M
Vervoordeldonk
J
J
Pfeilschifter
F
F
Märki
H
H
van den Bosch
D000818:Animals; D002478:Cells, Cultured; D005576:Colforsin / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D016207:Cytokines / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology*; D015232:Dinoprostone / Q000096:biosynthesis* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005920:Glomerular Mesangium / Q000187:drug effects*; D015151:Immunoblotting; D010741:Phospholipases A / Q000096:biosynthesis* / Q000378:metabolism; D054467:Phospholipases A2; D011863:Radioimmunoassay; D051381:Rats
D016207:Cytokines; D005576:Colforsin; D003907:Dexamethasone; D010741:Phospholipases A; D054467:Phospholipases A2; D015232:Dinoprostone