Molecular cloning and sequence analysis of cDNA and genomic DNA for the human cone transducin alpha subunit.
A novel GTP binding protein (G protein) alpha subunit cDNA was isolated from a T cell leukemia cell line, Jurkat, utilizing polymerase chain reaction (PCR). The predicted amino acid sequence of this G protein alpha subunit showed the highest identity (96.6%) to bovine cone cell-specific transducin (Tc alpha). The organization of the coding region of this G protein alpha subunit gene was composed of 8 exons and 7 introns. Northern hybridization revealed the presence of this G protein message in a retinoblastoma cell line, Y79. In Jurkat, however, the message was detectable only by reverse transcription/PCR. Taken together, this novel G protein alpha subunit must be human Tc.
Kubo
M
M
Section of Bacterial Infection, Hokkaido University, Sapporo, Japan.
Hirano
T
T
Kakinuma
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry*; D006801:Humans; D015458:Leukemia, T-Cell / Q000235:genetics; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry / Q000235:genetics; D015293:Transducin / Q000737:chemistry / Q000235:genetics*
D009363:Neoplasm Proteins; D004247:DNA; D015293:Transducin