Crystallographic analysis of the neurophysin-oxytocin complex. A preliminary report.
Single crystals of a bovine neurophysin II-oxytocin complex have been obtained using (NH4)2SO4 as the precipitating agent. The crystals diffract to at least 2.7 A resolution, belong to Laue group 4/mmm and exhibit systematic absences consistent with either space group P4(1)2(1)2 or P4(3)2(1)2. The cell dimensions are a = b = 69.07 A and c = 113.26 A. The crystals contain one neurophysin-oxytocin dimer per asymmetric unit. Based on a Vm of 2.9 A3/Da, the solvent content is calculated to be 58%. Chromatographic analysis of the dissolved crystals suggests the presence of three oxytocin molecules per neurophysin dimer.
Department of Crystallography, University of Pittsburgh, PA 15260.
J P
JP
Rose
E
E
Breslow
H B
HB
Huang
B C
BC
Wang
D000818:Animals; D002417:Cattle; D009481:Neurophysins / Q000737:chemistry*; D014961:X-Ray Diffraction
D009481:Neurophysins