Ovarian steroid modulation of [3H]muscimol binding in the spinal cord of the rat.
[3H]Muscimol binding was measured in the lumbar spinal cord of female rats by in vitro quantitative autoradiography. Ovariectomized rats were treated subcutaneously with either oil, estradiol benzoate (EB) or EB plus progesterone (P) in a regime known to reliably induce sexual receptivity. The level of [3H]muscimol binding was highest in laminae I-III and in the region around the central canal. Binding was lower in laminae IV-VI and was frequently undetectable in the ventral horn. There was a significant increase in the level of binding in laminae I-III after EB treatment. There was also a significant increase after treatment with EB+P in comparison to both the ovariectomized and EB-treated groups in this same region of the spinal cord.
Rockefeller University, New York, NY 10021.
M M
MM
McCarthy
H
H
Coirini
M
M
Schumacher
D W
DW
Pfaff
B S
BS
McEwen
S
S
Schwartz-Giblin
D000818:Animals; D001345:Autoradiography; D004338:Drug Combinations; D004958:Estradiol / Q000502:physiology*; D005260:Female; D009118:Muscimol / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism*; D011374:Progesterone / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000378:metabolism*; D014316:Tritium
D004338:Drug Combinations; D014316:Tritium; D009118:Muscimol; D011374:Progesterone; D004958:Estradiol