Complementation of snf1, a mutation affecting global regulation of carbon metabolism in yeast, by a plant protein kinase cDNA.
A cDNA, cRKIN1, encoding a putative homologue of the yeast (Saccharomyces cerevisiae) SNF1-encoded protein-serine/threonine kinase, has been isolated from a library prepared from rye endosperm mRNA. Northern blot analysis demonstrated the presence of cRKIN1-related transcripts in developing endosperms but not in shoots, and Southern blot analysis showed the presence of a small gene family. SNF1 plays a central role in carbon catabolite repression in yeast and expression of the RKIN1 sequence in yeast snf1 mutants restored SNF1 function. This suggests that the RKIN1 protein has a role in the control of carbon metabolism in endosperms of rye.
Department of Biochemistry and Physiology, Institute of Arable Crops Research Harpenden, Hertfordshire, United Kingdom.
A
A
Alderson
P A
PA
Sabelli
J R
JR
Dickinson
D
D
Cole
M
M
Richardson
M
M
Kreis
P R
PR
Shewry
N G
NG
Halford
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002244:Carbon / Q000378:metabolism*; D003001:Cloning, Molecular; D015870:Gene Expression; D017343:Genes, Plant; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012434:Secale / Q000235:genetics*; D016415:Sequence Alignment
D002244:Carbon; D011494:Protein Kinases