Simple determination of tin in biological materials by atomic absorption spectrometry with a graphite furnace.
A simple and rapid determination of tin in biological materials (blood, brain, liver, kidney, etc,) by atomic absorption spectrometry (AAS) with a graphite furnace is described. Suppression of interferences by direct addition of ascorbic acid in wet-ashed biological materials was investigated for the determination of tin. This method involves no extraction with organic solvent for removal of the interferences, and therefore it is neither time consuming nor error prone, and is suitable for large sample sizes and small sample volumes. The limit of detection of this method is 0.002 microgram/mL, equivalent to a limit of detection in the materials of 0.02 microgram/g. The calibration graph is linear up to 1 microgram/mL of tin.
National Institute of Hygienic Sciences, Osaka Branch, Japan.
T
T
Itami
M
M
Ema
H
H
Amano
H
H
Kawasaki
D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry; D001923:Brain Chemistry; D006108:Graphite; D006358:Hot Temperature; D007501:Iron / Q000737:chemistry; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D051381:Rats; D013054:Spectrophotometry, Atomic / Q000379:methods; D014001:Tin / Q000032:analysis* / Q000097:blood
D014001:Tin; D006108:Graphite; D007501:Iron; D001205:Ascorbic Acid