Characterization of a synaptosomal ATP diphosphohydrolase from the electric organ of Torpedo marmorata.
A true ecto-apyrase (ATP diphosphohydrolase, EC 3.6.1.5) enzyme was found in the synaptosomal fraction from the electric organ of the electric ray Torpedo marmorata. The activity could not be attributed to the combined action of different enzymes. The pH requirement and calcium dependence were the same for hydrolysis of both substrates ADP and ATP. The enzyme had an apparent Km value of 117 microM for ATP and of 123 microM for ADP. The involvement of nonspecific phosphatases in the hydrolysis of both substrates was excluded. The enzyme hydrolyses almost equally well different nucleoside di- and triphosphates. ATP and ADP hydrolysis was not inhibited by seven ATPase inhibitors, i.e., sodium azide, dinitrophenol, ruthenium red, oligomycin, ouabain, sodium orthovanadate and lanthanum.
Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul Porto Alegre, RS, Brasil.
J J
JJ
Sarkis
C
C
Salto
D000818:Animals; D001081:Apyrase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent; D002458:Cell Fractionation; D002501:Centrifugation, Zonal; D004492:Edetic Acid / Q000494:pharmacology; D004557:Electric Organ / Q000201:enzymology*; D007700:Kinetics; D012265:Ribonucleotides / Q000378:metabolism; D013379:Substrate Specificity; D013574:Synaptosomes / Q000201:enzymology*; D014101:Torpedo
D002413:Cations, Divalent; D012265:Ribonucleotides; D004492:Edetic Acid; D001081:Apyrase; D002118:Calcium