Characterization of CD44 antigen during lymphoid ontogeny.
We studied immunohistological and biochemical aspects of the CD44 molecule with a mAb produced in our lab: GRHL-1. The characteristic expression of this antigen in cells of B lineage was analyzed. This mAb showed identical immunohistological patterns of reactivity to other mAbs included in CD44 cluster, on a variety lymphoid and nonlymphoid human tissues, and demonstrated similar bands on SDS-PAGE of 125I labeled lymphocyte lysates. This antigen is limited to cells of mature phenotype, and disappears in proliferating B cells in the germinal centers of the lymphoid follicles. CD44 is absent in pre-B and Burkitt cell lines. PKC activation mediate in vitro differentiation of pre-B cell lines. However, it is not involved in up-regulation of CD44 antigen expression.
Servicio de Análisis Clínicos e Inmunología, Hospital Virgen de las Nieves, Universidad de Granada, Spain.
A
A
Collado
A
A
de Andres
E
E
Cañadas
F
F
Ruiz-Cabello
O
O
Gomez
S
S
Pedrinaci
F
F
Garrido
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000276:immunology*; D001402:B-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D001792:Blood Platelets / Q000737:chemistry; D002454:Cell Differentiation / Q000276:immunology; D002461:Cell Line, Transformed / Q000737:chemistry; D004912:Erythrocytes / Q000737:chemistry; D006098:Granulocytes / Q000737:chemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007371:Interferon-gamma / Q000494:pharmacology; D008099:Liver / Q000737:chemistry; D011233:Precipitin Tests; D012867:Skin / Q000737:chemistry; D013601:T-Lymphocytes / Q000737:chemistry; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D007371:Interferon-gamma