Colchicine inhibition of duodenal absorption of calcium.
1. The effect of colchicine on calcium absorption across rat duodenum has been investigated using the single-pass continuous perfusion technique and the two-compartment system model. 2. Perfusing the rat duodenum with 0.1 and 0.5 mM colchicine produced a dose-dependent inhibiting pattern of calcium transport with no effect noted for water transport. 3. Colchicine at 0.5 mM caused a significant decrease in the rate of calcium uptake and in the accumulation capacity of the duodenal cells. 4. Accumulation of calcium in the duodenal strips displayed saturation kinetics with increasing concentration of calcium in the incubation medium. Colchicine at 0.5 mM showed a lower saturation level and decreased the average maximal flux around 46%.
Department of Physiology, Faculty of Medicine, American University of Beirut, Lebanon.
C F
CF
Nassar
L E
LE
Abdallah
N
N
Nuwayri-Salti
E G
EG
Karkaji
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D003078:Colchicine / Q000494:pharmacology*; D004386:Duodenum / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005260:Female; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000187:drug effects*; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D003078:Colchicine; D002118:Calcium