ATP is required for in vitro assembly of MHC class I antigens but not for transfer of peptides across the ER membrane.
We have translated the HLA-B27 heavy chain in vitro and studied its assembly with beta 2-microglobulin and peptide in microsomes from human cells. The assembly process requires ATP. However, the translocation of peptide across the endoplasmic reticulum (ER) membrane does not require ATP, and binding of biotinylated peptide to BiP, an ER luminal protein, occurs after ATP depletion. Proteinase K treatment of the microsomes does not block peptide translocation. Thus, ATP is required in the lumen of the ER for efficient assembly to occur. Microsomes prepared from Raji and T1 cells show similar levels of assembly, whereas assembly in T2 microsomes is 10-fold lower. This difference remains after peptide stimulation of assembly. The inefficient assembly in T2 microsomes is not due to impaired peptide translocation across the ER membrane, as no difference was found compared with microsomes from T1 cells. Instead, the defect seems to reside in the lumen of the ER.
Ludwig Institute for Cancer Research, Stockholm, Sweden.
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Lévy
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Gabathuler
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Larsson
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Kvist
D000255:Adenosine Triphosphate / Q000378:metabolism*; D001693:Biological Transport, Active / Q000502:physiology; D001710:Biotin / Q000378:metabolism; D002460:Cell Line; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D019286:Endopeptidase K; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D015796:HLA-B27 Antigen / Q000378:metabolism*; D006801:Humans; D009980:Influenza A virus / Q000378:metabolism; D046911:Macromolecular Substances; D008861:Microsomes / Q000378:metabolism; D009698:Nucleoproteins / Q000378:metabolism; D014176:Protein Biosynthesis / Q000502:physiology; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D012697:Serine Endopeptidases / Q000378:metabolism; D001613:beta 2-Microglobulin / Q000378:metabolism*
D015796:HLA-B27 Antigen; D046911:Macromolecular Substances; D009698:Nucleoproteins; D001613:beta 2-Microglobulin; D001710:Biotin; D000255:Adenosine Triphosphate; D012697:Serine Endopeptidases; D019286:Endopeptidase K