Long-term high-titer neutralizing activity induced by octameric synthetic HIV-1 antigen.
A titer for homologous viral neutralization activity (greater than 1:19,683) was observed after a 3.5-year immunization period with an octameric, branching peptide representing the principal neutralizing determinant (PND) of the human immunodeficiency virus-1IIIB envelope protein. Booster immunizations elicited persistent and potent antibodies in guinea pigs, exceeding responses produced by a conventional bovine serum albumin conjugate by 100-fold. Peptide length, central presentation of a conserved sequence, and inclusion of an upstream sequence contributed to immunogenicity. Titers (greater than 1:1,000) of heterotypic neutralizing antibodies also developed. Octameric PND peptides are a promising approach for an acquired immunodeficiency syndrome (AIDS) vaccine.
Wang
C Y
CY
United Biomedical, Inc., Lake Success, NY 11042.
Looney
D J
DJ
Li
M L
ML
Walfield
A M
AM
Ye
J
J
Hosein
B
B
Tam
J P
JP
Wong-Staal
F
F
D016915:AIDS Vaccines / Q000235:genetics / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D006168:Guinea Pigs; D015488:HIV Antigens / Q000235:genetics / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology*
D016915:AIDS Vaccines; D015488:HIV Antigens; D014614:Vaccines, Synthetic