Detection of the lipid-linked precursor oligosaccharide of N-linked protein glycosylation in Drosophila melanogaster.
The presence of a glycan of the same molecular size as the lipid linked precursor oligosaccharide (Glc3Man9GlcNAc2) of the N-linked protein glycosylation pathway in mammalian cells has been detected in a glycolipid fraction of cultured Drosophila melanogaster cells. Oligosaccharide sequencing studies were consistent with the existence of a glucosylated high mannose containing structure, which may be the common precursor for N-linked protein glycosylation in insect cells.
Department of Biochemistry, University of Oxford, UK.
G F
GF
Parker
P J
PJ
Williams
T D
TD
Butters
D B
DB
Roberts
D000818:Animals; D002460:Cell Line; D004331:Drosophila melanogaster / Q000378:metabolism*; D006017:Glycolipids / Q000737:chemistry / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D011499:Protein Processing, Post-Translational
D006017:Glycolipids; D006023:Glycoproteins; D009844:Oligosaccharides