Induction of macrophage inflammatory protein-1 beta gene expression in human monocytes by lipopolysaccharide and IL-7.
HuMIP-1 beta is a member of a gene family of inflammatory cytokines whose expression is induced by proinflammatory and mitogenic stimuli. The gene was rapidly induced in human peripheral blood monocytes by either LPS or IL-7. However, IL-7 was unable to induce HuMIP-1 beta mRNA in peripheral blood T cells. The induction of HuMIP-1 beta mRNA in monocytes by IL-7 or LPS was inhibited by IL-4. The 5'-regulatory region of the HuMIP-1 beta gene was cloned and sequenced. An analysis of this sequence revealed three consensus-binding sites for the nuclear factor PU.1 and three potential glucocorticoid response elements. However, the HuMIP-1 beta gene appears to be unresponsive to dexamethasone. Also present in the 5'-regulatory region was a LPS-responsive element located within 455 bp 5' to the start of transcription.
Department of Molecular Biology, Immunex Corporation, Seattle, WA 98101.
S F
SF
Ziegler
T W
TW
Tough
T L
TL
Franklin
R J
RJ
Armitage
M R
MR
Alderson
D001483:Base Sequence; D054407:Chemokine CCL4; D016207:Cytokines / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005809:Genes, Regulator; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology; D015851:Interleukin-7 / Q000494:pharmacology*; D008070:Lipopolysaccharides; D019402:Macrophage Inflammatory Proteins; D008969:Molecular Sequence Data; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D015846:Monokines / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D011971:Receptors, Immunologic / Q000032:analysis; D020395:Receptors, Interleukin-7
D054407:Chemokine CCL4; D016207:Cytokines; D015851:Interleukin-7; D008070:Lipopolysaccharides; D019402:Macrophage Inflammatory Proteins; D015846:Monokines; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D020395:Receptors, Interleukin-7; D015847:Interleukin-4