Effects of vanadate, ouabain and amiloride on the contraction of the rat testicular capsule to oxytocin.
1. The modification of the contraction of the rat testicular capsule to oxytocin (OT) by vanadate (0.7, 7 and 70 microM), ouabain (0.1 mM), and amiloride (10 microM to 1 mM) have been studied. 2. OT (1 nM-6 microM) and vanadate (10 microM-3 mM) induced contraction of the rat testicular capsule in a dose-dependent way (ED50: 188 +/- 66 nM and 82.8 +/- 7.4 microM, respectively). 3. Vanadate (0.7, 7 and 70 microM) and ouabain (0.1 mM) increases the contractile effect of OT (50 and 200 nM). 4. Amiloride (10 microM-1 mM) inhibit, in a dose-dependent way, the OT-contraction. 5. Amiloride (10 microM or 50 microM) block the ouabain but not the vanadate potentiation to OT.
Departamento de Medicina, Facultad de Medicina, Universidad de Oviedo, Spain.
M
M
Sanchez
F
F
Andrés-Trelles
A
A
Hidalgo
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D004347:Drug Interactions; D066298:In Vitro Techniques; D007474:Ion Exchange; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D010042:Ouabain / Q000494:pharmacology; D010121:Oxytocin / Q000494:pharmacology*; D051381:Rats; D012964:Sodium / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000502:physiology; D014638:Vanadates / Q000494:pharmacology*
D014638:Vanadates; D010121:Oxytocin; D010042:Ouabain; D000584:Amiloride; D012964:Sodium; D002118:Calcium