The serotoninergic system of the brain of the viper, Vipera aspis. An immunohistochemical study.
Serotoninergic cell bodies and fibers in the brain of the viper, Vipera aspis, were visualized by immunohistochemistry. Immunoreactive cell bodies were observed in the diencephalic hypothalamic periventricular organ and in the dorsal wall of the infundibular recess, in the nuclei raphe superior and inferior of the midbrain and hindbrain, and to a lesser extent in the nuclei reticularis superior, reticularis inferior and reticularis lateralis. In contrast to other reptilian species, serotoninergic cells were also observed in the central gray matter of the midbrain in the neighbourhood of the nucleus of the trochlear nerve. Immunoreactive fibers are widely distributed throughout the brain of the viper. In the olfactory bulb, fibers were observed in the internal plexiform layer and mitral cell layer. The cerebral cortex contains the highest density of fibers in the dorsal region. The distribution of immunoreactive fibers in the dorsal ventricular ridge is extremely heterogeneous, and five subcomponents of this structure can be distinguished. The majority of diencephalic and mesencephalic structures that contain immunoreactive fibers are also primary visual centres: the nuclei geniculatus lateralis pars dorsalis, the n. posterodorsalis and n. opticus tegmenti, and the optic tectum. Serotoninergic fibers in the nuclei of the oculomotor and motor cranial nerves (III, IV, V, VII, X) are disposed in a tightly woven basket around the non-immunoreactive cell bodies of the motoneurons. These findings, together with the available literature, suggest that the serotoninergic system in snakes is comparable to that in lizards, with a massive ascending projection of fibers from the n. raphe superior to mesencephalic and prosencephalic structures, and a descending projection from the n. raphe inferior to the spinal cord.
Laboratoire de Neuromorphologie, U-106 INSERM, Paris.
E
E
Challet
J
J
Pierre
J
J
Repérant
R
R
Ward
D
D
Miceli
D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D004027:Diencephalon / Q000648:ultrastructure; D007124:Immunoenzyme Techniques; D008636:Mesencephalon / Q000648:ultrastructure; D009411:Nerve Endings / Q000648:ultrastructure; D009412:Nerve Fibers / Q000648:ultrastructure; D012249:Rhombencephalon / Q000648:ultrastructure; D012701:Serotonin / Q000502:physiology*; D012911:Snakes / Q000033:anatomy & histology*; D013687:Telencephalon / Q000648:ultrastructure
D012701:Serotonin