Large-scale physical mapping within the region 22q12.3-13.1 in meningioma.
The lack of physical mapping data strongly restricts the analysis of the meningioma chromosomal region that was assigned to the bands 22q12.3-qter. Recently, we reported a new marker D22S16 for chromosome 22 that was assigned to the region 22q13-qter by in situ hybridization. Utilizing somatic cell hybrids we now sublocalized the marker D22S16 within the band region 22q12-13.1, thus placing it in the vicinity of the gene for the platelet derived growth factor (PDGFB). A physical map was established for the regions surrounding the PDGFB gene and the D22S16 marker. By means of pulsed-field gel electrophoresis (PFGE) D22S16 and PDGFB were found to be physically linked within 900 kb. We also identified two CpG clusters bordering the PDGFB gene. For the enzyme NotI, a variation of the PDGFB restriction pattern was found between different individuals. PFGE analysis of the two loci (PDFGB and D22S16) failed to identify major rearrangements in meningioma.
Herzog
R
R
University of Saarland, Department of Human Genetics, Homburg/Saar, Germany.
Gottert
E
E
Henn
W
W
Zang
K
K
Blin
N
N
Trent
J
J
Meese
E
E
D000818:Animals; D015139:Blotting, Southern; D002478:Cells, Cultured; D002871:Chromosome Banding; D002892:Chromosomes, Human, Pair 22; D004273:DNA, Neoplasm / Q000235:genetics* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology; D008577:Meningeal Neoplasms / Q000235:genetics* / Q000473:pathology; D008579:Meningioma / Q000235:genetics* / Q000473:pathology; D051379:Mice; D009693:Nucleic Acid Hybridization; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D015183:Restriction Mapping; D012867:Skin / Q000473:pathology
D004273:DNA, Neoplasm; D005819:Genetic Markers; D010982:Platelet-Derived Growth Factor