Quantitation of HIV: correlation with clinical, virological, and immunological status.
A quantitative assay has been used to measure titres of infectious HIV in peripheral blood of symptomatic and asymptomatic patients. Viral titres were assessed in conjunction with virological and immunological status of patients including measurement of p24 antigen, antibody responses to structural (gp41, p24) and regulatory gene products (NEF, REV, TAT, and VIF), determination of beta 2 microglobulin levels and enumeration of lymphocyte subsets. Titres of HIV were significantly higher among symptomatic than asymptomatic patients. Viral load was closely associated with the number of CD4+ cells, the proportion of these cells harbouring HIV increasing with disease progression. Higher titres of infectious HIV among symptomatic patients was also associated with p24 antigenaemia and decreased antibody responses to NEF.
Department of Virology, United Medical School, Guys Hospital, London, England.
S
S
O'Shea
T
T
Rostron
A S
AS
Hamblin
S J
SJ
Palmer
J E
JE
Banatvala
D000386:AIDS-Related Complex / Q000276:immunology / Q000382:microbiology*; D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000382:microbiology*; D002478:Cells, Cultured; D006678:HIV / Q000302:isolation & purification*; D015483:HIV Antibodies / Q000276:immunology; D015488:HIV Antigens / Q000276:immunology; D016655:HIV Core Protein p24 / Q000097:blood; D006679:HIV Seropositivity / Q000276:immunology / Q000382:microbiology*; D006801:Humans; D007958:Leukocyte Count; D016131:Lymphocyte Subsets; D001613:beta 2-Microglobulin / Q000032:analysis
D015483:HIV Antibodies; D015488:HIV Antigens; D016655:HIV Core Protein p24; D001613:beta 2-Microglobulin