Effects of cholesterol- or 7-ketocholesterol-containing liposomes on colony-forming ability of cultured cells.
Experiments with cultured Chinese hamster cells showed that incubation of the cells with (phosphatidylcholine + cholesterol + 7-ketocholesterol)-containing liposomes (4:3:1 by weight) during two hours led to a decrease in the colony-forming ability of cells down to zero, while (phosphatidylcholine + cholesterol)-containing liposomes (1:1 by weight) reduce this parameter by 90%. Furthermore, the cholesterol-containing liposomes (without 7-ketocholesterol) induce a decrease in the number of the maximal-site colonies accompanied by the corresponding increase in the number of the middle-size colonies.
Evolutionary Cytogerontology Sector, Faculty of Biology, Moscow State University, USSR.
A N
AN
Khokhlov
Prokhorov LYu
A S
AS
Ivanov
A I
AI
Archakov
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002784:Cholesterol / Q000737:chemistry*; D006224:Cricetinae; D003412:Cricetulus; D066298:In Vitro Techniques; D007653:Ketocholesterols / Q000737:chemistry*; D008081:Liposomes; D008563:Membrane Lipids / Q000737:chemistry / Q000502:physiology*
D007653:Ketocholesterols; D008081:Liposomes; D008563:Membrane Lipids; D002784:Cholesterol; C003001:7-ketocholesterol