Evidence for an association of the 15-kDa proteolipid of mediatophore with a 14-kDa polypeptide.
The present report shows that mediatophore, a nerve terminal membrane protein that translocates acetylcholine on calcium action, forms a complex with a 14-kDa polypeptide. The complex was identified based on the following results. (a) A polyclonal antimediatophore antiserum that immunoprecipitates activity precipitates both the 15- and 14-kDa polypeptides. (b) After HPLC purification of mediatophore, both antigens were found in the same peak. (c) After 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate solubilization of presynaptic membranes or of the purified mediatophore, an immunoaffinity column made with the anti-14-kDa antigen monoclonal antibody retained both the 14-kDa and the 15-kDa polypeptide. Similarly, immunoprecipitation experiments using protein A-coated beads sedimented an immunocomplex in which both antigens were found. (d) The 14-kDa antigen could be localized in the synaptosomal membrane where mediatophore and its 15-kDa component are found.
Département de Neurochimie, CNRS, Gif-sur-Yvette, France.
M
M
Israël
N
N
Morel
B
B
Lesbats
D000818:Animals; D000911:Antibodies, Monoclonal; D002793:Cholic Acids / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003902:Detergents / Q000494:pharmacology; D008566:Membranes / Q000378:metabolism; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011233:Precipitin Tests; D011510:Proteolipids / Q000737:chemistry / Q000378:metabolism*; D012995:Solubility; D013574:Synaptosomes / Q000378:metabolism; D014101:Torpedo
D000911:Antibodies, Monoclonal; D002793:Cholic Acids; D003902:Detergents; D009419:Nerve Tissue Proteins; D010455:Peptides; D011510:Proteolipids; C050299:mediatophore; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate