Storage and preparation of samples for erythropoietin radioimmunoassay.
The evaluation of a radioimmunoassay for erythropoietin developed using recombinant material as immunogen and radiotracer is presented. A series of serum samples prepared and stored under varying conditions showed that immunoreactive erythropoietin levels were stable at room temperature for at least 10 days and at -20 degrees C for 5 months. The optimum time for separating sera from samples was between 6 and 24 h after venepuncture. Serum EPO values were significantly higher than those measured in heparin or potassium EDTA plasma.
Department of Haematology, Leeds General Infirmary.
R G
RG
Kendall
C
C
Chapman
A E
AE
Hartley
D R
DR
Norfolk
D000906:Antibodies / Q000032:analysis; D000925:Anticoagulants / Q000494:pharmacology; D001793:Blood Preservation / Q000379:methods; D015925:Cryopreservation; D004921:Erythropoietin / Q000097:blood*; D006801:Humans; D007457:Iodine Radioisotopes; D011863:Radioimmunoassay; D011994:Recombinant Proteins / Q000097:blood / Q000187:drug effects
D000906:Antibodies; D000925:Anticoagulants; D007457:Iodine Radioisotopes; D011994:Recombinant Proteins; D004921:Erythropoietin