Different patterns of inhibition of adrenaline-induced platelet aggregation and kinetics in vivo by acetylsalicylic acid and indobufen.
The inhibitory effect of 50 mg/kg lysyl acetylsalicylic acid (ASA) intravenously injected 24 and 4 h before 80 micrograms/kg adrenaline, 40 mg/kg indobufen injected 15 min before, or a combination of ASA plus indobufen on the platelet aggregation and kinetics was evaluated in anaesthetized dogs previously injected with 111indium- (111In-) labelled platelets using gamma-camera dynamics studies. The highest degree of inhibition, indicated by the lowest platelet aggregation ratio decrease and the most significant 111In-labelled platelet mobilization from hepatic and splenic stores with a corresponding increase of labelled and unlabelled platelet counts in blood, was obtained in ASA-treated dogs. Such changes were less marked when ASA plus indobufen was injected. In indobufen-treated dogs a similar mobilization of 111In-labelled platelets with an increase in circulating platelet numbers was evident only after the second adrenaline injection. It is concluded that platelet mobilization is mainly dependent on the production of an ASA-sensitive substance.
Physiological Haemostasis Centre, Oporto Medical School, Portugal.
J S
JS
Fortunato
M J
MJ
Pinheiro
M C
MC
Monteiro
M A
MA
Rodrigues
I
I
Amaral
D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects; D004285:Dogs; D004837:Epinephrine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007275:Injections, Intravenous; D054833:Isoindoles; D007700:Kinetics; D008099:Liver / Q000187:drug effects; D008297:Male; D010654:Phenylbutyrates / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D010976:Platelet Count / Q000187:drug effects; D011433:Propranolol / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects
D054833:Isoindoles; D010654:Phenylbutyrates; D010975:Platelet Aggregation Inhibitors; C020371:indobufen; D011433:Propranolol; D001241:Aspirin; D004837:Epinephrine