Antigen detection in invasive aspergillosis.
The application of a reverse passive latex agglutination test and dot-blot assay are reported in the diagnosis of 50 proven cases of invasive aspergillosis and 28 suspected cases. At a latex titre cut off of greater than or equal to 1 in 8 the test had a sensitivity of 29.4%, specificity of 96.3% and efficiency of 41.4%. This was in sera taken when the diagnosis was first suggested. The sensitivity rose to 55.1% when sera with the maximum level of antigen were examined. The dot blot was more sensitive with 33.3% of cases being positive in the initial sera. This increased to 61.5% when the serum with the maximum antigen level was taken.
Department of Medical Microbiology, Medical School, University of Manchester, U.K.
J P
JP
Burnie
D000372:Agglutination Tests / Q000379:methods; D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology; D000946:Antigens, Fungal / Q000032:analysis*; D001228:Aspergillosis / Q000175:diagnosis / Q000276:immunology*; D001232:Aspergillus fumigatus / Q000276:immunology; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D011817:Rabbits; D015203:Reproducibility of Results; D012463:Saliva / Q000382:microbiology; D012680:Sensitivity and Specificity
D000908:Antibodies, Fungal; D000946:Antigens, Fungal