Reversible inhibition of the urea cycle and gluconeogenesis by N,N-diethyl-m-toluamide.
Addition of DEET to suspensions of rat hepatocytes completely abolished synthesis of urea from ammonia and production of glucose from lactate. Both activities were fully restored after cells were washed and resuspended in DEET-free medium. These results suggest the DEET-induced impairment is a reversible biochemical defect at a site common to both pathways.
Brini
C M
CM
Department of Biochemistry and Biophysics, University of Rhode Island, Kingston 02881.
Tremblay
G C
GC
D000641:Ammonia / Q000378:metabolism; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003671:DEET / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005943:Gluconeogenesis / Q000187:drug effects*; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D014508:Urea / Q000378:metabolism*
D003671:DEET; D000641:Ammonia; D014508:Urea