Lipid-peptide interactions between fragments of the transit peptide of ribulose-1,5-bisphosphate carboxylase/oxygenase and chloroplast membrane lipids.
The interactions of fragments of the transit peptide of ribulose-1,5-bisphosphate carboxylase/oxygenase with lipid monolayers was studied in order to investigate the possible involvement of the membrane lipids in the protein import process. The fragments are surface active and have a differential ability to insert in lipid monolayers. The fragments have a preference for the chloroplast galacto- and sulpholipids and phosphatidylglycerol and interact with envelope membrane lipid extracts. These results suggest that probably transit peptide-lipid interactions are involved in the chloroplast protein import process.
van 't Hof
R
R
Centre for Biomembranes and Lipid Enzymology, State University of Utrecht, The Netherlands.
Demel
R A
RA
Keegstra
K
K
de Kruijff
B
B
D000595:Amino Acid Sequence; D001692:Biological Transport; D002736:Chloroplasts / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D007887:Fabaceae / Q000737:chemistry / Q000201:enzymology; D008563:Membrane Lipids / Q000737:chemistry / Q000502:physiology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000502:physiology*; D010743:Phospholipids / Q000378:metabolism; D010946:Plants, Medicinal; D011498:Protein Precursors / Q000737:chemistry / Q000502:physiology; D012273:Ribulose-Bisphosphate Carboxylase / Q000502:physiology*
D008563:Membrane Lipids; D010446:Peptide Fragments; D010743:Phospholipids; D011498:Protein Precursors; D012273:Ribulose-Bisphosphate Carboxylase