Sphingosine and unsaturated fatty acids modulate the base exchange enzyme activities of rat brain membranes.
The base exchange enzymes catalyze the incorporation of L-serine, ethanolamine and choline into their corresponding phospholipids. The L-serine base enzyme activity was increased 120% by 0.1 mM sphingosine. There was a modest increase of the ethanolamine base exchange enzyme activity but the choline base exchange enzyme activity was unaffected. Na-arachadonate, Na-oleate and Na-linolenate at 0.2 mM concentration increased the activity of the L-serine and ethanolamine base exchange enzymes but inhibited the choline base exchange enzyme activity. A model is proposed suggesting that modulations of the L-serine base exchange enzyme may participate in the regulation of the calcium phospholipid-dependent protein kinase C.
Department of Biochemistry and Molecular Biology, University of Manitoba, Faculty of Medicine, Winnipeg, Canada.
J N
JN
Kanfer
D
D
McCartney
D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000648:ultrastructure; D002462:Cell Membrane / Q000201:enzymology*; D002794:Choline / Q000378:metabolism; D004075:Diglycerides / Q000502:physiology; D019856:Ethanolamine; D004983:Ethanolamines / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000502:physiology*; D008954:Models, Biological; D009711:Nucleotides / Q000502:physiology; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D012694:Serine / Q000378:metabolism; D013110:Sphingosine / Q000494:pharmacology*
D004075:Diglycerides; D004983:Ethanolamines; D005231:Fatty Acids, Unsaturated; D009711:Nucleotides; D010743:Phospholipids; D012694:Serine; D019856:Ethanolamine; D002794:Choline; D013110:Sphingosine