Physical map of human Xq27-qter: localizing the region of the fragile X mutation.
We describe a physical map of the end of the long arm of the human X chromosome encompassing the region from Xq27.2 to the q telomere, inclusive of the chromosomal band Xq28. This region is of particular interest, since it contains the highest density of genes associated with genetic diseases. The map covers a total of 12 megabases (Mb) of DNA and extends from the telomere to 3 Mb beyond the most likely position of the fragile X mutation, defined by a cluster of translocation breakpoints in somatic cell hybrids. The map determines order and position of loci throughout the Xq28 region and localizes cell line breakpoints marking the fragile X region to an interval of 300-700 kilobases between 8 and 8.7 Mb proximal of the Xq telomere.
Deutsches Krebsforschungszentrum, Institut für Virusforschung, Heidelberg, Federal Republic of Germany.
A
A
Poustka
A
A
Dietrich
G
G
Langenstein
D
D
Toniolo
S T
ST
Warren
H
H
Lehrach
D015139:Blotting, Southern; D002874:Chromosome Mapping; D015342:DNA Probes; D005600:Fragile X Syndrome / Q000235:genetics*; D006801:Humans; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D014960:X Chromosome / Q000648:ultrastructure*
D015342:DNA Probes