Twenty-one percent partial hepatectomy. In vivo rat model for the study of liver regeneration.
We describe a new model of submaximal stimulation of liver regeneration in the rat. Removal of the right lateral lobes in the rat produced a 21% hepatectomy. The measurement accuracy of the incorporation rate of [14C]thymidine into DNA 24 h after 21% hepatectomy (n = 32) was less than that after the standard 34% hepatectomy (n = 32), with S.D./mean being 35% and 130%, respectively (F-test, p less than 0.025). This model was able to detect regeneration-stimulation activity present in liver cytosol extract and serum of 68% hepatectomized rats. Using this model we identified a subfraction of rat serum achieved after treatment of serum with ethanol and ion-exchange column, which had a highly stimulatory effect. Stimulation obtained with serum from hepatectomized pigs showed that these factors are not species specific.
Department of Medicine B, Rambam Medical Center, Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
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Y
Baruch
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Frith
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Kirsch
D000818:Animals; D004247:DNA / Q000096:biosynthesis; D006498:Hepatectomy; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D008954:Models, Biological; D051381:Rats; D013552:Swine
D004247:DNA