Idiotype specific T-cell lines inducing experimental systemic lupus erythematosus in mice.
Immunization of mice with either antibodies bearing the 16/6 idiotype (16/6 Id) or anti-idiotypic antibodies against the 16/6 Id induces experimental systemic lupus erythematosus (SLE). We report here the establishment and characterization of 16/6 Id-specific T-cell lines from C3H.SW (H-2b) and BALB/c (H-2d) mice. Both lines proliferate specifically in response to the 16/6 Id in an H-2-restricted manner. The injection of 16/6 Id-specific T cells into syngeneic mice led to the development of experimental SLE. Furthermore, inoculation of the 16/6 Id-specific T-cell line derived from C3H.SW mice into the H-2 compatible C57BL/6 mice, which are non-responders to the 16/6 Id, induced experimental SLE. This report provides direct evidence for the role of idiotype-specific T cells in the induction of experimental SLE.
Fricke
H
H
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Mendlovic
S
S
Blank
M
M
Shoenfeld
Y
Y
Ben-Bassat
M
M
Mozes
E
E
D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000032:analysis; D001327:Autoimmune Diseases / Q000276:immunology*; D002460:Cell Line; D005260:Female; D005455:Fluorescent Antibody Technique; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007668:Kidney / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology*
D000936:Antigen-Antibody Complex; D007130:Immunoglobulin Idiotypes