The isolation and characterization of a calmodulin-encoding gene (CMD1) from the dimorphic fungus Candida albicans.
Candida albicans is a dimorphic, opportunistic pathogen of humans, and calcium and calmodulin have been implicated in its morphogenic transition. The C. albicans calmodulin-encoding gene, CMD1, was isolated from cDNA and genomic phage lambda libraries using the Saccharomyces cerevisiae CMD gene as a hybridization probe. Southern-blot hybridization analysis of genomic DNA suggests the existence of only one type of calmodulin gene in C. albicans. Comparison of cDNA and genomic sequences identified a 222-bp intron located immediately after the Met start codon. The predicted amino acid sequence was 60% identical with yeast CMD and 70% identical with CMDs of filamentous fungi and vertebrates. We have localized the CMD1 gene to chromosome 3 using the contour-clamped homogeneous electric field gel electrophoresis. The CMD1 gene hybridized to a single 650-nucleotide transcript which was present in equivalent amounts in both the yeast and hyphal forms of the organism.
Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine 92717.
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D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002147:Calmodulin / Q000235:genetics*; D002176:Candida albicans / Q000235:genetics*; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D016415:Sequence Alignment
D002147:Calmodulin; D004271:DNA, Fungal; D004247:DNA