Standardized HPLC/DAD system, based on retention indices and spectral library, applicable for systematic toxicological screening.
An HPLC gradient elution system is applied for general toxicological screening. The use of a diode array detector with library software allows storage of retention parameters, UV spectra, and peak quality parameters of examined substances. The developed library is used for automatic identification of compounds. The retention indices of 225 substances are measured in the 1-nitroalkane scale. These data may be of interlaboratory use because of an appropriate correction procedure. Long-term reproducibilities of retention behavior of 1-nitroalkanes and correction standards are satisfactory. The conditions of interlaboratory use are discussed.
Bogusz
M
M
Institute of Forensic Medicine, Karl-Ruprecht University, Heidelberg, Germany.
Wu
M
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D064420:Drug-Related Side Effects and Adverse Reactions; D006838:Hydrocarbons; D006863:Hydrogen-Ion Concentration; D009574:Nitro Compounds; D004364:Pharmaceutical Preparations / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D015813:Substance Abuse Detection / Q000379:methods
D006838:Hydrocarbons; D009574:Nitro Compounds; D004364:Pharmaceutical Preparations