Remarkably enhanced expression of the type II hexokinase in rat hepatoma cell line AH130.
Expression of mRNAs encoding hexokinase isozymes was studied in various cells such as rat brain, liver, skeletal muscle, kidney and heart, and the rat hepatoma cell line AH130 by Northern blotting. High specific expression of type II hexokinase was observed only with AH130 cells. In contrast, specific expression of type I hexokinase was detected in energy-requiring normal tissue cells such as brain and heart. These results suggest that the expression of hexokinase isozyme in the tumor cells is different from that in normal cells.
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Y
Y
Shinohara
J
J
Ichihara
H
H
Terada
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA; D006593:Hexokinase / Q000235:genetics* / Q000378:metabolism; D008114:Liver Neoplasms, Experimental; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D051381:Rats; D014407:Tumor Cells, Cultured
D004247:DNA; D006593:Hexokinase