Fibronectin and basement membrane components in renal amyloid deposits in patients with primary and secondary amyloidosis.
Kidney biopsies from one patient with primary (AL) and three with secondary (AA) amyloidosis were used for an ultrastructural study of the collocalization of basement membrane proteins and the extracellular matrix protein fibronectin within amyloid deposits. Antibodies against amyloid P component, laminin, and heparan sulphate proteoglycan core protein all reacted with the basement membranes and the amyloid depositions in AA and AL amyloidosis. Monoclonal and polyclonal antibodies against collagen type IV reacted only with the basement membranes. Anti-fibronectin reaction was found in association with the basement membranes in all four cases, while labelling of amyloid depositions was found only in one of the AA amyloid cases and in the AL amyloid depositions. It is concluded that basement membrane components may be of importance for the formation of amyloid fibrils.
Department of Pathology, University of Linköping, Sweden.
G T
GT
Westermark
B
B
Norling
P
P
Westermark
D000686:Amyloidosis / Q000378:metabolism*; D001485:Basement Membrane / Q000378:metabolism*; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism*; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D007674:Kidney Diseases / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism; D000685:Serum Amyloid A Protein / Q000378:metabolism; D000683:Serum Amyloid P-Component / Q000378:metabolism
D011508:Chondroitin Sulfate Proteoglycans; D003094:Collagen; D005353:Fibronectins; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D007797:Laminin; D000685:Serum Amyloid A Protein; D000683:Serum Amyloid P-Component