Synthesis and characterization of a Pertussis toxin-biotin conjugate.
We prepared a Pertussis toxin-biotin conjugate and found its biological properties to be similar to those of native Pertussis toxin with respect to the hemagglutination, Chinese hamster ovary cell, and lymphocyte proliferation assays. Direct binding to Chinese hamster ovary and Jurkat cells was observed using fluorescence microscopy. Pertussis toxin-biotin was also found to possess similar glycoconjugate binding specificities as those of 125I-labeled Pertussis toxin.
Heerze
L D
LD
Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada.
Clark
C G
CG
Chen
Y
Y
Smith
R H
RH
Armstrong
G D
GD
D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D005777:Geese; D006023:Glycoproteins / Q000494:pharmacology; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella / Q000378:metabolism / Q000494:pharmacology*
D006023:Glycoproteins; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin