Suspended mouse peritoneal macrophages. Preparation and properties.
Since macrophages (MPH) are able to adhere firmly to solid surfaces, the recovery of viable and functional MPH has proven to be extremely difficult. We have developed a simple method using agarose coating for preparing MPH and culturing the cells in suspension. Their properties were tested over 72 h. The oxidative burst declined with time, but could be restored using the lymphokine rich supernatant of pokeweed-stimulated mouse spleen cells. In contrast, phagocytosis and Candida intra-cellular killing remained unchanged.
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INSERM U65, Département de Biologie-Santé, Université de Montpellier II, France.
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D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods*; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D008163:Luminescent Measurements; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D010587:Phagocytosis; D016897:Respiratory Burst; D012685:Sepharose; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors
D012685:Sepharose; D013755:Tetradecanoylphorbol Acetate