Hepatic expression of apolipoprotein A-I gene in rats is upregulated by monounsaturated fatty acid diet.
The effect of the degree of dietary fat saturation on the hepatic expression of apolipoprotein A-I mRNA was studied in male rats. Animals were maintained for two months on a high fat diet (40% w/w) containing 0.1% cholesterol. Two groups of control animals received either chow diet or chow plus 0.1% cholesterol, while experimental groups received their fat supplement as coconut, corn or olive oil respectively. Dietary cholesterol did not affect apolipoprotein A-I mRNA levels as compared to control animals. Corn oil fed animals had significantly higher levels of hepatic apolipoprotein A-I mRNA than those receiving cholesterol, or coconut oil plus cholesterol. Olive oil fed animals had significantly higher levels of hepatic apolipoprotein A-I mRNA when compared to all other dietary groups. Our data indicate that monounsaturated fatty acids supplied as olive oil play a major role in regulating the hepatic expression of apolipoprotein A-I in male rats.
Osada
J
J
Lipid Metabolism Laboratory, U.S.D.A. Human Nutrition Center on Aging, Tufts University, Boston, MA 02111.
Fernandez-Sanchez
A
A
Diaz-Morillo
J L
JL
Aylagas
H
H
Miro-Obradors
M J
MJ
Ordovas
J M
JM
Palacios-Alaiz
E
E
D000818:Animals; D016632:Apolipoprotein A-I / Q000235:genetics*; D015152:Blotting, Northern; D002791:Cholesterol, Dietary / Q000494:pharmacology; D015342:DNA Probes; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D004587:Electrophoresis, Agar Gel; D015870:Gene Expression; D008099:Liver / Q000378:metabolism*; D008297:Male; D010938:Plant Oils / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D015854:Up-Regulation
D016632:Apolipoprotein A-I; D002791:Cholesterol, Dietary; D015342:DNA Probes; D004042:Dietary Fats, Unsaturated; D010938:Plant Oils; D012333:RNA, Messenger