Induction of alpha interferon by human immunodeficiency virus type 1 in human monocyte-macrophage cultures.
The induction of interferon (IFN) by human immunodeficiency virus type 1 (HIV-1) in primary, nonstimulated monocyte-macrophage cultures was studied. HIV-1 infection, as confirmed by p24 antigen levels in the cell supernatant, led to the production of alpha interferon (IFN-alpha) over 7 to 21 days following infection. In two of seven experiments, the IFN detected was acid labile. Coupled reverse transcription-polymerase chain reaction analysis confirmed the induction of IFN-alpha mRNA in cells of HIV-1-infected cultures.
Szebeni
J
J
National Cancer Institute, Bethesda, Maryland 20892.
Dieffenbach
C
C
Wahl
S M
SM
Venkateshan
C N
CN
Yeh
A
A
Popovic
M
M
Gartner
S
S
Wahl
L M
LM
Peterfy
M
M
Friedman
R M
RM
D002448:Cell Adhesion; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D016898:Interferon-alpha / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D007700:Kinetics; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D009000:Monocytes / Q000276:immunology* / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D014158:Transcription, Genetic
D016898:Interferon-alpha; D012333:RNA, Messenger