Specific binding of neoglycoproteins to Toxoplasma gondii tachyzoites.
Several studies have shown that protozoa bind to glycoproteins or neoglycoproteins. Here we report that Toxoplasma gondii binds strongly to bovine serum albumin-glucosamide. The binding was rapid, time dependent, partially reversible, saturable, and specific. Scatchard analysis showed about 40,000 molecules of bovine serum albumin-glucosamide per toxoplasma cell. The apparent dissociation constant was found to be 4.46 x 10(-8) M.
Laboratoire de Parasitologie, Faculté de Pharmacie, UFR de Sciences Médicales et Pharmaceutiques, Angers, France.
R
R
Robert
P L
PL
de la Jarrige
C
C
Mahaza
J
J
Cottin
A
A
Marot-Leblond
J M
JM
Senet
D000818:Animals; D006023:Glycoproteins / Q000378:metabolism*; D007457:Iodine Radioisotopes; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014122:Toxoplasma / Q000378:metabolism*
D006023:Glycoproteins; D007457:Iodine Radioisotopes; D012710:Serum Albumin, Bovine