Ricin B-chain promotes the internalisation of liposomal contents into rat hepatoma cells.
Ricin B-chain covalently attached to liposomes has been shown to promote the binding of the liposomes to rat hepatoma cells through its galactosyl binding site. Internalisation of the bound liposomes is demonstrated by the cytotoxicity of methotrexate-containing liposomes, the transfection of cells with targeted liposomes containing pSV2-neo DNA and the intracellular activity of an enzyme encapsulated in liposomes targeted with the ricin B-chain.
FRD-UCT Research Centre for Molecular Biology, Department of Biochemistry, University of Cape Town, Rondebosch, South Africa.
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Friede
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von Holt
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D003850:Deoxyribonuclease I; D008081:Liposomes / Q000737:chemistry*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D008563:Membrane Lipids / Q000737:chemistry; D008727:Methotrexate; D008855:Microscopy, Electron, Scanning; D011487:Protein Conformation; D051381:Rats; D012276:Ricin / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D008081:Liposomes; D008563:Membrane Lipids; D012276:Ricin; D003850:Deoxyribonuclease I; D008727:Methotrexate