Adhesion of Bordetella pertussis to sulfatides and to the GalNAc beta 4Gal sequence found in glycosphingolipids.
The adherence of the human respiratory pathogen, Bordetella pertussis, to purified glycosphingolipids was investigated using thin layer chromatography overlay assays. Both virulent and avirulent strains of B. pertussis bound to asialo GM1. The bacterium did not bind to the gangliosides GM1, GD1a, GD1b, and GT1b, nor to lactosylceramide, trihexosylceramide, globoside, or Forssman antigen. However, after treatment of the chromatography plates with sialidase, B. pertussis bound to the gangliosides GM1, GM2, GD1a, GD1b, and GT1b but not to GM3. Comparison of the oligosaccharide structures of these gangliosides suggests that the minimum sugar structure needed for avid bacterial binding is GalNAc beta 4Gal. This structure has been previously implicated as a receptor for other human respiratory pathogens (Krivan, H. C., Roberts, D. D., Ginsburg, V. (1988) Proc. Natl. Acad. Sci. U.S.A 85, 6157-6161). Virulent strains of B. pertussis also bound specifically to sulfatide. This response was dose-dependent and inhibited by the anionic polysaccharide dextran sulfate. The sulfated-sugars dextran sulfate, fucoidan, and heparin inhibited the attachment of virulent strains of B. pertussis to human WiDr cells and to hamster trachea cells indicating that sulfatides on the surface of mammalian cells may function as a receptor for B. pertussis. The occurrence of both sulfatides and asialo GM1 in human lung and trachea suggests that these glycolipids may serve as specific receptors for B. pertussis.
Brennan
M J
MJ
Division of Bacterial Products, Food and Drug Administration, Bethesda, Maryland 20892.
Hannah
J H
JH
Leininger
E
E
D000818:Animals; D001422:Bacterial Adhesion; D001886:Bordetella pertussis / Q000378:metabolism* / Q000472:pathogenicity; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D006224:Cricetinae; D005677:G(M1) Ganglioside; D006028:Glycosphingolipids / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D013433:Sulfoglycosphingolipids / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014774:Virulence
D006028:Glycosphingolipids; D013433:Sulfoglycosphingolipids; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside