Amplification of the secretory IgA response to Toxoplasma gondii using cholera toxin.
Our study demonstrates that cholera toxin (CT) markedly enhances the intestinal anti-T. gondii antibody response following oral immunisation of mice with a T. gondii sonicate (TSo) and CT. The antibodies induced were mostly IgA and secretory IgA but a small quantity of IgG was also produced. In contrast, no intestinal anti-T. gondii IgM antibodies were detected. Anti-CT IgA antibodies were also present in intestinal secretions but in much lower quantities than the T. gondii-specific IgA. No anti-CT IgG nor IgM antibodies were detected. Western blot analysis showed that CT induced not only an increase of the intensity of the intestinal IgA antibody response to the 30-kDa band but also induced intestinal IgA antibodies against other major T. gondii proteins (p22, and the 28-kDa antigen) as recognised by specific monoclonal antibodies. The amplification of the anti-T. gondii secretory IgA response by means of an appropriate adjuvant may be one major step leading towards an orally induced immune protection against toxoplasmosis.
Bourguin
I
I
Unité Université-INRA d'Immunologie Parasitaire, UFR des Sciences Pharmaceutiques de Tours, Centre INRA de Tours, Nouzilly, France.
Chardes
T
T
Mevelec
M N
MN
Woodman
J P
JP
Bout
D
D
D000276:Adjuvants, Immunologic; D000284:Administration, Oral; D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000276:immunology; D015153:Blotting, Western; D002458:Cell Fractionation; D002772:Cholera Toxin / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis* / Q000276:immunology; D007422:Intestines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014122:Toxoplasma / Q000276:immunology*; D015164:Vaccines, Inactivated
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D007071:Immunoglobulin A, Secretory; D015164:Vaccines, Inactivated; D002772:Cholera Toxin