Purification of Phleum pratense pollen extract by immunoaffinity chromatography and high-performance ion-exchange chromatography.
Basic allergens of Phleum pratense pollen extract have been purified by either sequence gel filtration-ion-exchange high-performance liquid chromatography (HPIEX) and size-exclusion HPLC or sequence gel filtration-immunoaffinity chromatography and HPIEX. The second procedure seems to be suitable for preparative purposes.
Dipartimento di Chimica Organica e Industriale, Università di Milano, Italy.
E
E
Bolzacchini
P
P
Di Gennaro
G
G
Di Gregorio
B
B
Rindone
P
P
Falagiani
G
G
Mistrello
I
I
Sondergaard
D000485:Allergens / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007120:Immunochemistry; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D007164:Immunosorbents; D007525:Isoelectric Focusing; D011058:Pollen / Q000737:chemistry*
D000485:Allergens; D007164:Immunosorbents