Turnover of the transferrin receptor is not influenced by removing most of the extracellular domain.
We treated intact cells with trypsin to remove most of the external domain of the transferrin receptor and investigated what effect the absence of the external domain had on the turnover of the fragment that remained associated with the cells. To detect the cell-associated tryptic fragment, which contains a small amount of the external domain, the transmembrane domain, and the cytoplasmic domain, we prepared an anti-peptide antibody against a segment of the cytoplasmic domain. This antibody specifically immunoprecipitated the intact transferrin receptor as well as a 21-kDa peptide from trypsin-treated HeLa cells. Several lines of evidence indicated that the 21-kDa peptide was the cell-associated tryptic fragment of the transferrin receptor. The fragment was only present in trypsin-treated cells; the fragment migrated as a dimer in nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis, as it should if it were derived from the transferrin receptor; a goat antibody prepared to the purified human transferrin receptor also precipitated the 21-kDa peptide from trypsinized cells. In addition, treating the tryptic fragment with neuraminidase increased the electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gels, suggesting the fragment contained O-linked carbohydrate. When cells were trypsinized and then incubated at 37 degrees C, the half-life of the tryptic fragment (15 +/- 4 h) was not significantly different than the half-life of the intact receptor (19 +/- 6 h). This indicates that removing 95% of the external domain of the transferrin receptor has little effect on processes operating in the turnover of the receptor.
Rutledge
E A
EA
Molecular and Cell Biology Program, University of Texas, Dallas, Richardson 75083-0688.
Mikoryak
C A
CA
Draper
R K
RK
D000595:Amino Acid Sequence; D003593:Cytoplasm; D004220:Disulfides; D005110:Extracellular Space; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D011233:Precipitin Tests; D011990:Receptors, Transferrin / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014357:Trypsin / Q000494:pharmacology
D004220:Disulfides; D010446:Peptide Fragments; D011990:Receptors, Transferrin; D014357:Trypsin