Expression of alpha B-crystallin in the developing rat kidney.
The expression and cellular localization of alpha B-crystallin during rat renal development was studied by Northern blot analysis and by immunocytochemistry. Northern blotting of total RNA extracted from whole kidneys revealed that the messenger RNA for alpha B-crystallin rapidly increased after birth to reach adult levels by 20 days. At the same time, immunohistochemistry for alpha B-crystallin demonstrated that the prominent elongation of Henle's loop during the first 10 days of life was accompanied by increased alpha B-crystallin expression. Thus, the development of alpha B-crystallin expression is correlated temporally with the acquisition of tubule function in early post-natal life.
Department of Pathology, Columbia University College of Physicians and Surgeons, New York, New York.
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T
Iwaki
A
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Iwaki
R K
RK
Liem
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JE
Goldman
D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern; D003459:Crystallins / Q000235:genetics / Q000378:metabolism*; D005333:Fetus / Q000378:metabolism*; D007150:Immunohistochemistry; D007668:Kidney / Q000196:embryology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D003459:Crystallins; D012333:RNA, Messenger