Measurement of cell proliferation by enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody to bromodeoxyuridine.
An ELISA was developed and optimized to measure cell proliferation using a monoclonal antibody to bromodeoxyuridine (BrdUrd). Incorporation of BrdUrd into myoblast monolayers, measured as the optical density at 492 nm, increased in response to fetal calf serum, IGF-I and EGF, the ELISA data correlated closely with data obtained by BrdUrd immunocytochemistry (r = 0.984), cell counting (r = 0.972) and tritiated thymidine uptake by liquid scintillation counting (r = 0.990). The BrdUrd ELISA is a useful alternative to measurement of tritiated thymidine uptake by scintillation counting, and has the added advantages of dispensing with the use of radioactivity and of being less labour intensive.
Department of Medicine, Medical School, University of Newcastle upon Tyne, UK.
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Perros
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Weightman
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001973:Bromodeoxyuridine / Q000276:immunology / Q000378:metabolism*; D002452:Cell Count; D002455:Cell Division; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D009132:Muscles / Q000166:cytology* / Q000378:metabolism; D013552:Swine
D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine