Conformational transitions and hydration of poly d(A-T).poly d(A-T) in fibers.
Conformational transitions of poly d(A-T).poly d(A-T) have been studied by fiber X-ray diffraction and measurement of fiber dimensions. Results obtained for the D-A-B and D-B transitions are presented and analyzed. For all these form transitions, cooperativity effects are observed for the variation of the rise per nucleotide versus the relative humidity. Detailed information about hydration of the polynucleotide during form transitions and the numbers of water molecules per nucleotide necessary to stabilize the different helical conformations are presented.
Laboratoire de Biophysique Moléculaire, UA CNRS 494, Université de Nancy I, Faculté des Sciences, Vandoeuvre Les Nancy, France.
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Abouelkassimi
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Albiser
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Premilat
D006813:Humidity; D046911:Macromolecular Substances; D009690:Nucleic Acid Conformation; D011067:Poly dA-dT / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry; D014867:Water / Q000737:chemistry*; D014961:X-Ray Diffraction
D046911:Macromolecular Substances; D014867:Water; D011067:Poly dA-dT; D012965:Sodium Chloride