Defect of a fiber cell-specific 94-kDa protein in the lens of inherited microphthalmic mutant mouse Elo.
Deficiency in a 94,000-dalton protein in the non-crystallin fraction from the Elo mouse lens was shown. To perform further investigations, we raised an antibody against the 94,000-dalton protein isolated from normal mouse lens. Western blot analysis with the antibody indicated that the protein was only present in the lens and not in the brain, lung, heart, liver, and kidney. In the lens, it was unique to the cortex and nucleus fractions, not being present in the epithelial cells. Furthermore, it was observed in the water-soluble fraction as well as in the urea-soluble fraction. The antibody weakly but clearly reacted with the chick CP97 lens peptide, a fiber cell-specific protein, and anti-CP97 antibody also reacted with the 94,000-dalton protein. From these results, we concluded that the protein corresponds to CP97 cytoskeletal protein in the mouse lens. The protein was deficient in the lenses from Elo mice, but microphthalmic lenses from CTA mice contained a normal level.
Masaki
S
S
Department of Biochemistry, Institute for Developmental Research, Aichi, Japan.
Tamai
K
K
Shoji
R
R
Watanabe
T
T
D000818:Animals; D000906:Antibodies; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D007908:Lens, Crystalline / Q000737:chemistry*; D051379:Mice; D008817:Mice, Mutant Strains; D008850:Microphthalmos / Q000235:genetics / Q000378:metabolism*; D008970:Molecular Weight; D009928:Organ Specificity; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification*; D012016:Reference Values
D000906:Antibodies; D011506:Proteins