A follow-up study of sperm preparation for IVF by swim-up in a solution of hyaluronate.
Spermatozoa prepared for in-vitro fertilization (IVF) by swim-up in a balanced salt solution containing hyaluronate gave rates of fertilization, cleavage and pregnancy which were not significantly different from those obtained with sperm prepared by swim-up in standard IVF medium followed by centrifugation. However, the content of prostaglandin F2alpha in the final sperm suspension was higher using hyaluronate but this seemed to be of no consequence for IVF. Thus, preparation of normal sperm samples for IVF may be simplified by performing swim-up in a balanced salt solution containing hyaluronate.
University of Göteborg, Department of Obstetrics and Gynaecology, Sahlgrenska University Hospital, Sweden.
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P
Sjöblom
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M
Wikland
D002469:Cell Separation / Q000379:methods; D002498:Centrifugation; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005500:Follow-Up Studies; D006801:Humans; D006820:Hyaluronic Acid; D007248:Infertility, Male / Q000503:physiopathology; D008297:Male; D012996:Solutions; D013081:Sperm Motility
D012996:Solutions; D006820:Hyaluronic Acid