[The determination of ampicillin in the blood and urine of patients by microcolumn high-performance liquid chromatography].
The method of the quantitative determination of ampicillin in the blood serum and urine on the Soviet-made chromatograph "Milichrom" with an ultraviolet detector and 2 x 62 cm column is described. The conditions of chromatography were as follows: wavelength-210 nm; elution rate-100 microliters/min; analysis time-12 min. The conditions of chromatographic analysis were selected on sorbents Lichrosorb RP-18, Nucleosil S-18 and Silosorb S-18. The given method of ampicillin determination--is characterized by a high specificity, accuracy, the use of small volumes of the blood taken from a finger, the rapidity of making analyses and may be recommended for introduction in the clinical practice for optimization of treating patients with ampicillin.
N I
NI
Komarova
N S
NS
Krylova
G I
GI
Lushanova
T A
TA
Chimitova
V V
VV
Chernysheva
D000667:Ampicillin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005069:Evaluation Studies as Topic; D006801:Humans; D015203:Reproducibility of Results
D000667:Ampicillin
Opredelenie ampitsillina v krovi i moche bol'nykh metodom mikrokolonochnoĭ vysokoéffektivnoĭ zhidkostnoĭ khromatografii.