Liquid chromatographic separation of phenothiazines and structurally-related drugs using a beta-cyclodextrin bonded phase column.
The retention behaviour of 16 phenothiazines and structurally-related drugs on a beta-cyclodextrin bonded phase column is examined with respect to pH, methanol and TEAA concentrations in the mobile phase, and column temperature. The results are utilized to optimize the separation of these compounds. Both isocratic and gradient-elution separation of the 16 phenothiazine derivatives on the beta-cyclodextrin bonded phase column are investigated. The successful separation of all of the compounds is achieved in the gradient-elution mode.
Department of Chemistry, McGill University, Montreal, Quebec, Canada.
S
S
Li
W C
WC
Purdy
D002853:Chromatography, Liquid / Q000379:methods; D003505:Cyclodextrins; D006863:Hydrogen-Ion Concentration; D010640:Phenothiazines / Q000302:isolation & purification*; D013329:Structure-Activity Relationship; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D010640:Phenothiazines; D047392:beta-Cyclodextrins; C031215:betadex