Cloning vectors and antibiotic-resistance markers for Brevibacterium sp. R312.
Replication of several cryptic plasmids from coryneform strains was investigated in Brevibacterium sp. R312. Only the Corynebacterium glutamicum pSR1 replicon was found to be suitable for establishing a host-vector system. Two pSR1 derivatives, pRPCG200 and pHYCG1, were used as cloning vectors. They carry a neomycin-resistance-encoding and a tetracycline-resistance-encoding gene, respectively.
Chaire de Microbiologie Industrielle et de Génétique des Microorganismes, ENSA, Montpellier, France.
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D001951:Brevibacterium / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D003352:Corynebacterium / Q000235:genetics; D004261:DNA Replication / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D009355:Neomycin / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics*; D013752:Tetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance / Q000235:genetics
D004269:DNA, Bacterial; D015500:Chloramphenicol O-Acetyltransferase; D013752:Tetracycline; D009355:Neomycin