The spliced leader gene of Angiostrongylus cantonensis.
A 5' leader sequence has been identified on mRNAs of the parasitic nematode Angiostrongylus cantonensis. A 720-bp XhoI restriction fragment containing the gene encoding the leader sequence has been cloned and sequenced. It contains a 22-nt sequence identical to that of the leader sequence of Caenorhabditis elegans, a consensus splice site and a putative Sm antigen binding site. The gene is present as a tandem repeating unit of approximately 60 copies, and unlike C. elegans it is not associated with the 5S ribosomal RNA gene. The SL-RNA is 110 nt long and the sequence and primer extension studies suggest that it is transcribed from the tandemly repeating SL gene. It is precipitable from cell-free extracts of adult nematodes by anti-Sm anti-sera, and from RNA by anti-TMG anti-sera, thus suggesting its inclusion with small nuclear ribonucleoproteins in RNA splicing.
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
G W
GW
Joshua
R Y
RY
Chuang
S C
SC
Cheng
S F
SF
Lin
R S
RS
Tuan
C C
CC
Wang
D000595:Amino Acid Sequence; D000801:Angiostrongylus / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D015152:Blotting, Northern; D002107:Caenorhabditis / Q000235:genetics* / Q000378:metabolism; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011233:Precipitin Tests; D012315:RNA Caps; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D051381:Rats; D014158:Transcription, Genetic
D012315:RNA Caps; D012333:RNA, Messenger