A simple method for ultrastructural enzyme cytochemistry on small volumes and numbers of isolated cells.
A simple technique is described for ultrastructural enzyme cytochemistry using small volumes and numbers of single cells. Small aliquots (1-5 microliters) of single cells suspended in a glutaraldehyde fixative are incubated on poly-L-lysine-coated glass coverslips. Cells in contact with the coated coverslip become immobilized to that support. All changes of solutions are accomplished without repeated centrifugation.
Department of Cell Biology, Neurobiology and Anatomy, Ohio State University, Columbus 43210.
J M
JM
Robinson
D015720:5'-Nucleotidase / Q000032:analysis*; D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D002460:Cell Line / Q000201:enzymology; D016591:Histocytological Preparation Techniques; D006801:Humans; D051379:Mice; D009000:Monocytes / Q000201:enzymology* / Q000648:ultrastructure; D011107:Polylysine
D011107:Polylysine; D000135:Acid Phosphatase; D015720:5'-Nucleotidase