A detailed study of the regulation and evolution of the two classes of patatin genes in Solanum tuberosum L.
The class-specific expression of patatin genes was investigated by analysing four new patatin genes. A class I patatin gene from cv. Berolina as well as a class I and two class II patatin genes from the monohaploid cultivar AM 80/5793 were isolated and partially sequenced. Sequence comparison indicates rearrangements as the major source for the generation of diversity between the different members of the classes. The expression of single genes was studied in potato plants transformed with chimaeric genes where the putative patatin promoters were fused to the GUS reporter gene. A detailed histochemical analysis reveals that both class I genes are expressed as the previously described class I patatin gene B33 from cv. Berolina [1], i.e. in the starch-containing cells of potato tubers and in sucrose-induced leaves. The class II gene pgT12 shows the same pattern as the previously described class II gene pgT2 [2], i.e. expression in root tips and in the vascular tissue of tubers, whereas no activity was detectable for pgT4. Thus the expression pattern of both classes of genes seems to be stable at least within or even between different cultivars.
Institut für Genbiologische Forschung Berlin GmbH, FRG.
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Liu
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Rocha-Sosa
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Hummel
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Willmitzer
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WB
Frommer
D001483:Base Sequence; D005075:Biological Evolution; D002265:Carboxylic Ester Hydrolases; D002678:Chimera; D004247:DNA; D005786:Gene Expression Regulation; D015698:Genomic Library; D006651:Histocytochemistry; D008969:Molecular Sequence Data; D005810:Multigene Family; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D011198:Solanum tuberosum / Q000235:genetics*; D014170:Transformation, Genetic
D010940:Plant Proteins; C043222:patatin protein, Solanum tuberosum; D004247:DNA; D002265:Carboxylic Ester Hydrolases