[Effect of benzodiazepines on NAD binding to synaptic membrane in the rat brain].
The receptor protein solubilized from synaptic membranes specifically binds [14C] NAD (dissociation constant--0.75 microM, capacity of binding sites--0.0125 nmol of metaid per 1 mg of protein). All the studied benzodiazepines (phenazepam, nitrazepam, clonazepam, flunitrazepam) are able to displace [14C] NAD from its receptor sites, the mixed type of inhibition being manifested. An inhibition constant for flunitrazepam, a ligand of benzodiazepine receptors, equals 10 microM. GABA promotes an inhibiting effect of benzodiazepines. It is supposed that neurotropic action of NAD is realized through the GABA-benzodiazepine complex of neuronal membranes.
A I
AI
Fomenko
P K
PK
Parkhomets
S P
SP
Stepanenko
G V
GV
Donchenko
D000818:Animals; D001569:Benzodiazepines / Q000494:pharmacology*; D001665:Binding Sites; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D009243:NAD / Q000378:metabolism*; D051381:Rats; D013570:Synaptic Membranes / Q000187:drug effects* / Q000378:metabolism
D008565:Membrane Proteins; D009243:NAD; D001569:Benzodiazepines
Vliianie benzodiazepinov na sviazyvanie NAD sinapticheskimi membranami golovnogo mozga krys.