The effect of wavelength on glial fibrillary acidic protein immunoreactivity in laser-induced lesions in rabbit retina.
Rabbit retinas were treated with low-intensity laser coagulation at five different wavelengths. Using an indirect immunocytochemical method, the retinas were stained for glial fibrillary acidic protein (GFAP) at 2, 4, 21 and 32 days after coagulation such that we could follow GFAP expression in the Müller cells during retinal repair. GFAP-positive staining was found in the end feet of the Müller cells at 2 days after laser coagulation. GFAP immunoreactivity was observed throughout the Müller cells, surrounding the central necrotic tissue, at days 4, 21 and 32 after laser coagulation. Scar tissue in the subretinal space at days 21 and 32, which was more pronounced for the longer wavelengths produced by the Krypton and Nd-YAG lasers, also showed GFAP immunoreactivity. The Müller cells remained GFAP-immunoreactive for at least 32 days after laser coagulation.
Department of Ophthalmology, University Hospital V.U.B. Laarbeeklaan 101, Brussels, Belgium.
M J
MJ
Tassignon
N
N
Stempels
J
J
Nguyen-Legros
F
F
De Wilde
M
M
Brihaye
D000818:Animals; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism / Q000528:radiation effects*; D007124:Immunoenzyme Techniques; D007834:Lasers / Q000009:adverse effects; D008028:Light Coagulation / Q000009:adverse effects; D011817:Rabbits; D012160:Retina / Q000378:metabolism* / Q000528:radiation effects; D012164:Retinal Diseases / Q000209:etiology / Q000378:metabolism*; D014945:Wound Healing
D005904:Glial Fibrillary Acidic Protein