Leukocyte filtration removes infectious particulate debris but not free virus derived from experimentally lysed HIV-infected cells.
We used in vitro model of storage-induced leukocyte degradation in blood to experimentally characterize the infectivity of the debris of lymphocytes harboring human immunodeficiency virus (HIV-1). The leukocyte filtration process removed both intact HIV-infected H9 cells and the particulate debris, but failed to remove the cell-free HIV released from lysed cells. These data suggest that the leukocyte filtration of donor blood soon after collection would optimally reduce the particulate infectious burden of blood for transfusions.
Rawal
B
B
Department of Laboratory Medicine, University of California, San Francisco.
Yen
T S
TS
Vyas
G N
GN
Busch
M
M
D001803:Blood Transfusion / Q000592:standards*; D002460:Cell Line / Q000382:microbiology / Q000648:ultrastructure; D015497:HIV-1 / Q000472:pathogenicity*; D006801:Humans; D007937:Leukapheresis / Q000592:standards*