Ovulation rate, follicle population and FSH levels in cyclic rats after administration of an inhibin-neutralizing antiserum.
Ovulation rate, follicle growth, serum FSH and oestradiol concentrations were studied after a single intraperitoneal injection of inhibin antiserum in 5-day-cyclic rats. Control rats received (non-immune) serum from castrated sheep or saline. Rats were injected at 10.00 h on dioestrus-1 (D1), i.e. the day following the day of oestrus, or at 17.00 h on dioestrus-2 (D2). The ovaries were excised at necropsy 48 h after injection, or at first or second oestrus after injection. After routine histology fresh corpora lutea were counted and/or differential follicle counts were made. Results from rats injected with either (non-immune) serum from castrated sheep or with saline were not different and were therefore combined to form the control group. The activity of inhibin-neutralizing antibodies in the circulation of antiserum-treated rats was reduced by approximately 39% between 8 h and second oestrus after injection, as determined by the binding of purified bioactive radioiodinated 31 kDa bovine inhibin. Rats were injected on D1 and killed at first oestrus. The number of fresh corpora lutea was significantly higher in antiserum-treated rats than in controls (13.9 +/- 0.4 vs 11.8 +/- 0.4; P less than 0.05). Other rats injected on D1 were killed either 48 h or at the second oestrus after injection. Blood was collected 8, 16, 24 and 48 h and at first and second oestrus after injection.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Anatomy, Medical Faculty, Erasmus University, Rotterdam, The Netherlands.
H J
HJ
Sander
P
P
Kramer
E C
EC
van Leeuwen
W A
WA
van Cappellen
H M
HM
Meijs-Roelofs
F H
FH
De Jong
D000818:Animals; D003338:Corpus Luteum / Q000502:physiology; D004958:Estradiol / Q000097:blood; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood*; D007106:Immune Sera / Q000008:administration & dosage; D007116:Immunization, Passive; D007265:Inhibins / Q000276:immunology / Q000502:physiology*; D009929:Organ Size; D006080:Ovarian Follicle / Q000502:physiology*; D010053:Ovary / Q000033:anatomy & histology; D010060:Ovulation / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
D007106:Immune Sera; D004958:Estradiol; D007265:Inhibins; D005640:Follicle Stimulating Hormone