Influence of burn-induced lipid-protein complex on IL1 secretion by PBMC in vitro.
The lipid-protein complex (LPC) formed by thermal injury to skin, which has been shown to have a toxic effect on mice, and which suppresses the immune response, was tested for its specific influence on monocytes. Growth of bacterial endotoxin-stimulated peripheral blood mononuclear cells (PBMC) was inhibited in the presence of LPC, however, the inhibition was less at the time of the optimal rate of cell proliferation. Inhibition was proportional to LPC concentration. ConA-stimulated PBMC were also inhibited by LPC in a dose-related manner. PBMC, in the presence of LPC, secreted interleukin 1 (IL1) at an increasing rate as LPC concentration rose from 5 to 40 micrograms/ml, and the levels of IL1 which could be induced by endotoxin were increasingly amplified in the presence of LPC. In comparison to LPC, the native tissue proteins which were isolated from unburned skin by the same techniques which produced LPC from burned skin, were tested for their effect on PBMC. Native proteins had no effect on IL1 secretion, whether on background or endotoxin-stimulated levels. Thus, the thermally induced change in skin proteins has a specific effect on monocyte IL1 secretion which is not matched by the native proteins, indicating that burn injury to skin specifically affects the lymphokine cascade and consequent immune function.
Monge
G
G
Zentrum für Lehre und Forschung, Kantonsspital, Basel, Switzerland.
Sparkes
B G
BG
Allgöwer
M
M
Schoenenberger
G A
GA
D000818:Animals; D002056:Burns / Q000276:immunology*; D002455:Cell Division; D002478:Cells, Cultured; D003208:Concanavalin A; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D008563:Membrane Lipids / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology*; D009000:Monocytes / Q000502:physiology*; D012867:Skin / Q000276:immunology
D007375:Interleukin-1; D008563:Membrane Lipids; D008565:Membrane Proteins; D003208:Concanavalin A