An improved test for Africanized honeybee mitochondrial DNA.
Mitochondrial DNA derived from Apis mellifera scutellata, the ancestor of the Africanized bees of the New World, lacks a BglII restriction site found in other types of honeybee. We present primers allowing amplification of a 485-bp section of the cytochrome b gene containing this site, using the polymerase chain reaction. Digestion of the amplified product with BglII yields contrasting patterns between Africanized and other honeybees.
Crozier
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YC
Department of Genetics & Human Variation, La Trobe University, Bundoora, Australia.
Koulianos
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S
Crozier
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RH
D000349:Africa; D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000235:genetics*; D003573:Cytochrome b Group / Q000235:genetics; D004272:DNA, Mitochondrial / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D015183:Restriction Mapping
D003573:Cytochrome b Group; D004272:DNA, Mitochondrial