Trichloroethylene interactions with muscle cells.
The toxic effect of trichlorethylene (TCE) was investigated on isolated muscles prepared from frog and rats. Twitch and tetanic contractions as well as caffeine-induced contractures, were recorded. Trichloroethylene at a concentration of 0.25-4.0 mM depressed the force development of both twitch and tetanic tension in a dose-dependent manner. This effect was not influenced by the type of muscle. As TCE shortened the time to peak of twitch contractions, it may alter the Ca2+ binding kinetics. Subthreshold caffeine concentrations applied after pre-exposure to TCE (1 or 2mM) induced contractures. The same TCE exposure enhanced regular caffeine contractures through increasing the speed of tension development and the absolute force. Exposure to 5 or 10 mM TCE did not affect the first caffeine-induced contracture but enhanced the potency of the second caffeine dose given 15 min after the first. The results suggest that the interaction of TCE with membrane sites is responsible for Ca2+ release for contractile processes.
Central Institute of Occupational Medicine, Dept. of Work Physiology, Berlin, Germany.
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Kössler
D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D009119:Muscle Contraction / Q000187:drug effects; D009132:Muscles / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011893:Rana esculenta; D051381:Rats; D011919:Rats, Inbred Strains; D013742:Tetanus / Q000139:chemically induced; D014241:Trichloroethylene / Q000009:adverse effects / Q000494:pharmacology*
D014241:Trichloroethylene; D002110:Caffeine; D002118:Calcium