Analysis of Moloney murine leukemia virus revertants mutated at the gag-pol junction.
Among Moloney murine leukemia viruses (Mo-MuLVs) having stop codons other than UAG at the gag-pol junction, Mo-MuLV with UAA, but not with UGA, had a replication disadvantage. Mo-MuLV with a glutamine codon (CAG) at the junction did not replicate. A revertant of this virus consisted of the original virus and a virus with a deletion of the pol region. Protease and Pr65gag encoded by their respective genomes complemented each other.
Department of Bacteriology, Faculty of Medicine, University of Tokyo, Japan.
T
T
Odawara
H
H
Yoshikura
M
M
Ohshima
T
T
Tanaka
D S
DS
Jones
F
F
Nemoto
Y
Y
Kuchino
A
A
Iwamoto
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002872:Chromosome Deletion; D003062:Codon / Q000235:genetics; D015750:Genes, gag; D015751:Genes, pol; D016679:Genome, Viral; D051379:Mice; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014162:Transfection
D003062:Codon; D009838:Oligodeoxyribonucleotides