Determination of hemoglobin through its peroxidase activity on chlorpromazine.
Chlorpromazine is an excellent chromogen for determining micro-quantities of hemoglobin. The oxidation of chlorpromazine by peroxidase activity of hemoglobin is coupled to a non-enzymatic reaction of second-order. Kinetic analysis of the overall system leads to a discussion about the optimal assay conditions. Spectrophotometric progress curves for the accumulation of the chlorpromazine cation radical during the reaction have been obtained, and further analyzed by non-linear regression. The use of a linear calibration curve of the enzymatic reaction rate against hemoglobin concentration is proposed for its determination.
Vázquez
A
A
Cátedra de Química, Escuela Universitaria Politécnica de Albacete, Universidad de Castilla-La Mancha, Spain.
Tudela
J
J
Varón
R
R
García-Cánovas
F
F
D002746:Chlorpromazine / Q000737:chemistry*; D004347:Drug Interactions; D006454:Hemoglobins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010544:Peroxidases / Q000737:chemistry*
D006454:Hemoglobins; D010544:Peroxidases; D002746:Chlorpromazine