Cloning and characterization of Daphnia mitochondrial DNA.
The mitochondrial genome of Daphnia pulex (Crustacea, Cladocera) was cloned as a single fragment into the plasmid vector pUC12. The genome size, estimated from restriction endonuclease fragment lengths, is 15,400 +/- 200 base pairs. The GC content, estimated from thermal denaturation studies, is 42%. The positions of 39 cleavage sites were mapped for 14 restriction enzymes. The distribution of these sites within the genome is random (P = 0.44). Heterologous hybridizations with Drosophila sylvestris mitochondrial DNA (mtDNA) probes indicate that gene orders within Daphnia and Drosophila mtDNAs are similar.
Department of Biological Sciences, University of Windsor, Canada.
D J
DJ
Stanton
T J
TJ
Crease
P D
PD
Hebert
D000818:Animals; D001482:Base Composition; D003001:Cloning, Molecular; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000378:metabolism; D003621:Daphnia / Q000235:genetics*; D004330:Drosophila / Q000235:genetics; D015183:Restriction Mapping
D004272:DNA, Mitochondrial