Presence of human immunoglobulin G anti serum pancreatic elastase 1 autoantibodies and their influence on elastase 1 radioimmunoassay.
Human immunoglobulin G (IgG) anti human pancreatic elastase 1 autoantibodies were detected in sera of patients with pancreatic disorders. The characteristics of these anti elastase 1 autoantibodies and their influence on radioimmunoassay (RIA) for elastase 1 were investigated. They were placed in the IgG class by the double antibody method, and most were assumed to be of a monoclonal type from their elution profiles in gel filtration analysis. The presence of autoantibodies in serum caused an increase in apparent elastase 1 values and a decrease in the recovery of elastase 1 exogenously added to the serum. These results suggest that elastase 1 immunoassay data for autoantibody positive sera can cause misjudgement of clinical stages of patients.
Shionogi Diagnostics Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
H
H
Asada
K
K
Shibata
K
K
Uchida
A
A
Yamauchi
M
M
Kono
Y
Y
Takeyama
H
H
Ohyanagi
Y
Y
Saitoh
D001323:Autoantibodies / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005188:False Negative Reactions; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000737:chemistry; D007457:Iodine Radioisotopes; D010182:Pancreatic Diseases / Q000201:enzymology* / Q000473:pathology; D010196:Pancreatic Elastase / Q000032:analysis / Q000276:immunology*; D011863:Radioimmunoassay
D001323:Autoantibodies; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D010196:Pancreatic Elastase