Post-column on-line HPLC measurement of reaction rates by using an open-closed derivatizing system.
An open-closed high-performance liquid chromatography (HPLC) assembly that enables post-column derivatization of the eluates and kinetic measurements (reaction-rate methods) with enhanced sensitivity and selectivity is constructed and tested by applying it to the separation and determination of the three main creatine kinase (CK) isoenzymes in serum samples with excellent results (consistent with those provided by the standard method) and recoveries between 95 and 105%.
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
M D
MD
Luque de Castro
J M
JM
Fernandez-Romero
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003402:Creatine Kinase / Q000097:blood*; D006801:Humans; D007527:Isoenzymes; D007700:Kinetics
D007527:Isoenzymes; D003402:Creatine Kinase