Interferon-alpha treatment of Daudi cells down-regulates the octamer binding transcription/DNA replication factors Oct-1 and Oct-2.
Treatment of Daudi cells with alpha-interferon (alpha-IFN) results in a considerable decrease in the levels of the octamer-binding DNA replication/transcription factors Oct-1 and Oct-2 and specifically inhibits gene expression by octamer-containing promoters. The inhibitory effect on octamer-binding proteins also occurs after culturing cells with phorbol 12-myristate 13-acetate but it does not occur following alpha-IFN treatment of an alpha-IFN-resistant variant of the Daudi cell line or of HeLa cells. We discuss the potential role of the decreased levels of octamer-binding proteins in the inhibition of cell proliferation.
Department of Biochemistry, University College and Middlesex School of Medicine, London, United Kingdom.
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D001483:Base Sequence; D002453:Cell Cycle; D004247:DNA / Q000096:biosynthesis; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D051863:Host Cell Factor C1; D006801:Humans; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000494:pharmacology*; D008969:Molecular Sequence Data; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; D009841:Oligonucleotides / Q000737:chemistry; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; C497692:HCFC1 protein, human; D051863:Host Cell Factor C1; D007370:Interferon Type I; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; D009841:Oligonucleotides; C494165:POU2F1 protein, human; C058435:POU2F2 protein, human; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014157:Transcription Factors; D004247:DNA