Direct measurement of the equilibrium between glutathione and dithiothreitol by high performance liquid chromatography.
The equilibrium constant between reduced glutathione (GSH), oxidized glutathione (GSSG), reduced dithiothreitol (DTTSHSH), and oxidized dithiothreitol (DTTSS) has been directly measured by high performance liquid chromatography analysis of equilibrium mixtures. The equilibrium constant at 25 degrees C for the reaction GSSG + DTTSHSH in equilibrium 2GSH + DTTSS varies from approximately 200 M, below pH 8, to approximately 2800 M, above pH 11. The observed pH dependence is generally consistent with published values of acid dissociation constants of these thiols.
Department of Biochemistry, Louisiana State University, Baton Rouge 70803-1806.
M H
MH
Chau
J W
JW
Nelson
D002851:Chromatography, High Pressure Liquid; D004220:Disulfides; D004229:Dithiothreitol / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics
D004220:Disulfides; D005978:Glutathione; D004229:Dithiothreitol