Improved monoclonal antibody tumor/background ratios with exchange transfusions.
Blood exchange transfusions were performed in nude rats with subcutaneous HTB77 human ovarian carcinoma xenografts in an attempt to improve specific monoclonal antibody (MoAb) tumor/non-tumor uptake ratios. Animals were injected intravenously with both 131I-5G6.4 specific and 125I-UPC-10 non-specific MoAb. Twenty-four hours later 65-80% of the original blood was exchanged with normal heparinized rat blood and then these rodents were sacrificed. Exchange transfusion significantly (P less than 0.05) decreased normal tissue activities of 131I (except for muscle) by 63-85%, while tumor activity decreased only 5%. Tumor to background ratios increased from 0.1-0.8 to 2.3-6.3. Exchange transfusions substantially enhance tumor/normal tissue antibody uptake ratios and, along with plasmapheresis, may be useful in enhancing antibody localization in vivo, particularly for therapy.
Henry
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CA
University of Michigan Medical Center, Department of Internal Medicine, Ann Arbor 48109-0028.
Clavo
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AC
Wahl
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D005078:Exchange Transfusion, Whole Blood; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000000981:diagnostic imaging* / Q000276:immunology; D010051:Ovarian Neoplasms / Q000000981:diagnostic imaging / Q000276:immunology; D016719:Radioimmunodetection; D014018:Tissue Distribution; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal