Fixed human tissues: a resource for the identification of individuals.
Polymorphic genetic loci of the deoxyribonucleic acid (DNA) present in formalin-fixed, paraffin-embedded tissues were successfully analyzed by utilizing the polymerase chain reaction. Using this analysis, with three different polymorphic loci [human leucocyte antigen (HLA) DQ alpha, low-density lipoprotein receptor, and parathyroid hormone], fixed tissues representing 14 different individuals were genotyped and could be distinguished from each other. The techniques were further applied to the fixed autopsy tissues of a man in which a question of paternity arose postmortem. Since many individuals have surgical procedures or autopsy, these readily available fixed tissues represent an additional resource for the identification of individuals.
Department of Pathology, University of Southern California School of Medicine, Los Angeles.
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Shibata
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Kurosu
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Noguchi
D000328:Adult; D000368:Aged; D000483:Alleles; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D016172:DNA Fingerprinting; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D005557:Formaldehyde; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000737:chemistry; D016612:Paraffin Embedding; D010281:Parathyroid Hormone / Q000235:genetics; D010334:Paternity; D016133:Polymerase Chain Reaction; D011973:Receptors, LDL / Q000235:genetics; D016707:Tissue Fixation
D006683:HLA-DQ Antigens; D010281:Parathyroid Hormone; D011973:Receptors, LDL; D005557:Formaldehyde; D004247:DNA