Screening for disorders of pyruvate metabolism by measuring the ratio of the rates of lactate production and pyruvate decarboxylation in cultured skin fibroblasts.
We assayed the rates of lactate production from [1-14C]pyruvate and decarboxylation of [1-14C]pyruvate in cultured skin fibroblasts from 8 patients with disorders of pyruvate metabolism and 16 control subjects. The disorders of pyruvate metabolism could be more readily detected by measuring the ratio between the rates of lactate production and pyruvate decarboxylation by cultured skin fibroblasts than by measuring either the rate in isolation.
Department of Pediatrics, School of Medicine, University of Tokushima, Japan.
Y
Y
Kuroda
I
I
Kawakami
H
H
Kobashi
E
E
Naito
M
M
Ito
T
T
Saijo
I
I
Yokota
E
E
Takeda
D000140:Acidosis, Lactic / Q000378:metabolism; D002478:Cells, Cultured; D003653:Decarboxylation; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D007773:Lactates / Q000096:biosynthesis*; D015323:Pyruvate Metabolism, Inborn Errors / Q000175:diagnosis*; D011773:Pyruvates / Q000378:metabolism*
D007773:Lactates; D011773:Pyruvates