Effect of hyperthermia on the in vitro hydrolysis of melphalan.
The pharmacokinetics of melphalan was investigated at 37 degrees C and 42 degrees C in vitro in canine and porcine plasma to assess heat-induced changes in the in vivo rate of melphalan hydrolysis. Melphalan concentrations were assayed using HPLC. Rate of spontaneous hydrolysis of melphalan at 42 degrees C was increased 1.5-fold in canine and 1.9-fold in porcine plasma. These results should be considered when interpreting in vivo disposition studies.
Riviere
J E
JE
Cutaneous Pharmacology and Toxicology Center, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
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R L
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Aucoin
D P
DP
Rogers
R A
RA
Williams
P L
PL
D000818:Animals; D004285:Dogs; D006358:Hot Temperature; D006868:Hydrolysis; D066298:In Vitro Techniques; D008558:Melphalan / Q000097:blood / Q000493:pharmacokinetics*; D013552:Swine
D008558:Melphalan