Age-related changes of supranucleosomal structures and DNA-synthesizing properties of rat liver chromatin.
The template activity, nucleosomal pattern and thermodenaturation parameters of transcriptionally low active (TLA) and transcriptionally active liver chromatin fractions were studied in adult (6-8 months) and old (26-28 months) rats. The following age-related changes of chromatin template properties were found: decrease of endogenous DNA polymerase alpha- and beta-activity in vitro in both chromatin fractions, and redistribution of newly synthetized RNA between mono- and oligonucleosomes in the TLA fraction. These changes are thought to be connected with age-related reorganization of chromatin structure, i.e. with increase of DNA-protein interactions, which stabilize association of chromatin supranucleosomal structures.
Institute of Gerontology AMS USSR, Kiev.
T G
TG
Mozzhukhina
V N
VN
Chabanny
E L
EL
Levitsky
Litoshenko AYa
D000375:Aging / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015870:Gene Expression; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure*; D009707:Nucleosomes / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic
D002843:Chromatin; D009707:Nucleosomes; D004247:DNA