Heparin-binding sites of rat rhabdomyosarcoma cells with low and high metastatic capacity.
Proteins able to bind the iduronate containing glycosaminoglycans: heparin, heparan sulfate and dermatan sulfate, were detected in strongly (RMS 0) and weakly (RMS 8) metastatic rat rhabdomyosarcoma cell lines. The 35S-methionine-labeled proteins solubilized from the cell membranes were chromatographed on Heparin-Ultrogel affinity column. The main retained protein migrated with an apparent molecular size of 19 kDa on polyacrylamide gel electrophoresis from both cell lines. The 19 kDa protein exhibited a higher affinity for iduronate containing glycosaminoglycans than for the glucuronate containing chondroitin sulfates. It was immunologically distinct from acid and basic fibroblast growth factors. The membranes of the RMS 8 cells contained about a two times higher amount of labeled 19 kDa protein than the membranes of the RMS 0 cells. The decreased amount of the labeled heparin-binding proteins in the highly metastatic cell line is in agreement with the previously evidenced decreased receptor-mediated binding of the iduronate containing glycosaminoglycans by these cells.
Laboratoire de Biochimie du Tissu Conjonctif, CNRS URA 1460, Créteil, France.
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D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D002846:Chromatography, Affinity; D005109:Extracellular Matrix / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006493:Heparin / Q000378:metabolism*; D008565:Membrane Proteins / Q000302:isolation & purification; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D012208:Rhabdomyosarcoma / Q000378:metabolism* / Q000556:secondary; D014407:Tumor Cells, Cultured / Q000378:metabolism
D006025:Glycosaminoglycans; D008565:Membrane Proteins; C013786:A73025; D006493:Heparin