Studies of the effect on tumor and normal cells in vitro with bioactive materials isolated from algae by a microcalorimetric method.
Recently much attention has been focused on the human physiological actions with bioactive materials from algae to enhance immunocompetence and to strengthen antineoplastic activity. In the study reported in this paper an MS 80 standard Calvet microcalorimeter was used for measuring the thermogram of HeLa, human breast carcinoma (Bcap-37) and diploid fibroblasts from human feral lung (2BS) under conditions with or without the presence of bioactive materials (Sp) from algae. At the same time, the cell number was counted, the inhibition rate of growth and the death rate were obtained. It has been shown that the Sp (100 micrograms/ml) was the growth inhibitor and lethal to tumor cells (Bcap-37 and HeLa), but had no influence on the normal (2BS) cells under the same conditions in vitro.
Liu
Z Q
ZQ
Department of Biology, Peking University, Beijing, China.
Cui
C H
CH
Zhang
Y M
YM
D001685:Biological Factors / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D002151:Calorimetry / Q000379:methods*; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D056890:Eukaryota / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D013816:Thermodynamics; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000473:pathology / Q000502:physiology
D001685:Biological Factors