Molecular cloning and nucleotide sequence analysis of a cDNA encoding pea cytosolic ascorbate peroxidase.
A cDNA clone encoding the cytosolic ascorbate peroxidase of pea (Pisum sativum L.) was isolated and its nucleotide sequence determined. While ascorbate peroxidase shares limited overall homology with other peroxidases, significant homology with all known peroxidases was found in the vicinity of the putative active site.
Department of Biochemistry and Microbiology, Cook College, Rutgers University, New Brunswick, NJ 08903-0231.
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R
Mittler
B A
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Zilinskas
D000595:Amino Acid Sequence; D060387:Ascorbate Peroxidases; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D003600:Cytosol / Q000201:enzymology; D004247:DNA / Q000235:genetics; D007887:Fabaceae / Q000201:enzymology / Q000235:genetics*; D015723:Gene Library; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics*; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D012333:RNA, Messenger; D004247:DNA; D010544:Peroxidases; D060387:Ascorbate Peroxidases