[A method for measuring the amount of retrogradely transported HRP in the rat masseteric motoneuron using Mesulam's HRP histochemical protocol and an image processing system. An investigation of the effect of dopamine receptor antagonists on the retrograde transport of HRP].
We developed a method for a determination of the amount of retrogradely transported HRP in the rat masseteric motoneuron using a modification of Mesulam's HRP histochemical protocol and an image processing system combined with a light microscope and a television camera. To test the validity and reproducibility of the new method, a quantitative comparison of the amount of dark blue granules of HRP-product in the cell body of masseteric motor neurons was performed between the right and left trigeminal motor nuclei of 70 rats, which resulted in no significant difference. An additional study used the method was made of the effects of administration of five dopamine receptor antagonists with different biochemical and pharmacologic properties on retrograde transport of HRP in the rat masseteric motoneuron. As a result, chlorpromazine, haloperidol, SCH 23390, and sulpiride significantly enhanced retrograde transport of HRP as against domperidone which showed no significant change in the transport. A possible regulatory system for retrograde transport of HRP in the masseteric motoneuron was discussed in relation to the action of the dopamine receptor.
Department of Oral Neuroscience, Kyushu Dental College, Kitakyushu, Japan.
K
K
Yoshino
S
S
Kawagishi
M
M
Iwamoto
N
N
Amano
D000818:Animals; D001369:Axons / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002746:Chlorpromazine / Q000494:pharmacology*; D018492:Dopamine Antagonists; D006220:Haloperidol / Q000494:pharmacology*; D006651:Histocytochemistry / Q000379:methods; D006735:Horseradish Peroxidase / Q000493:pharmacokinetics; D008297:Male; D008406:Masseter Muscle / Q000294:innervation*; D009046:Motor Neurons / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D018492:Dopamine Antagonists; D006735:Horseradish Peroxidase; D006220:Haloperidol; D002746:Chlorpromazine