On the toxinogenicity of Pseudomonas aeruginosa strains.
Investigation of 367 P. aeruginosa strains primarily isolated from clinical and other biological material as well as from the environment yielded results suggesting a substantial toxinogenic potential. 92.6% of the assayed culture filtrates derived from the strains under investigation proved positive in the early skin tests on rabbits. 49.7% of the assayed material induced cytotoxic alterations on Vero cells, the rates for Y1 and CHO cells being 50.3% and 43.5% respectively. 54.3% culture filtrates caused haemolysis of rabbit RBC and 52.7% lysed horse RBC. Gelatinase activity was found in 96.3% of tested material, protease in 89.8%, lecithinase in 62.4% and elastase in 29.6%. 12.6% of tested material induced fluid accumulation in a ligated intestinal loop. None of the culture filtrates elicited a positive reaction in the suckling mice test suggesting the absence of the thermostable enterotoxin.
Research Institute for Preventive Medicine, Bratislava, CSFR.
V
V
Majtán
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Hostacká
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A
Kosiarová
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L
Majtánová
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Kohútová
D002675:Child, Preschool; D003428:Cross Infection / Q000382:microbiology; D006461:Hemolysis; D006801:Humans; D007223:Infant; D008242:Lysogeny / Q000276:immunology; D011552:Pseudomonas Infections / Q000401:mortality; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000254:growth & development / Q000472:pathogenicity*