Expression of c-myc proto-oncogene during podophyllotoxin induced IW32 erythroleukemia cell differentiation.
The effect of hemin or podophyllotoxin on the differentiation of the erythropoietin (epo)-producing IW32 erythroleukemia cells was investigated. Podophyllotoxin induced IW32 cells to differentiate, and hemin potentiated the differentiation. Hemin had no effect on cell proliferation whereas podophyllotoxin inhibited cell growth. c-myc mRNA levels decreased biphasically by hemin or podophyllotoxin, while the combined treatment of hemin plus podophyllotoxin did not result in the initial decrease in c-myc mRNA level. Our data suggested that down-regulation of c-myc expression was not a prerequisite of IW32 cell differentiation induced by hemin and podophyllotoxin combined.
Institute of Biochemistry, Yang-Ming Medical College, Taipei, Taiwan.
Y T
YT
Yen
R T
RT
Wu
S J
SJ
Tang
F F
FF
Wang
D000818:Animals; D015152:Blotting, Northern; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc; D005914:Globins / Q000235:genetics; D006427:Hemin / Q000494:pharmacology; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D011034:Podophyllotoxin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D006427:Hemin; D005914:Globins; D011034:Podophyllotoxin