Protection against salicylate-induced hepatic injury by zinc. A histochemical and biochemical study.
Female Wistar rats received an oral dose of 700 mg salicylic acid/kg body wt., given as sodium salicylate. Some of the salicylate-treated rats received two subcutaneous injections of 100 mumol kg-1 ZnCl2 (24 h before and simultaneously with the salicylate administration). Other animals were given one subcutaneous injection of 100 mumol kg-1 ZnCl2 simultaneously with the salicylate treatment. Control rats were similarly injected with ZnCl2. Twenty four hours after salicylate treatment, serum and livers were taken for histochemical and biochemical analysis. The most remarkable effects of the treatment were enrichment of lipid droplets and iron and a reduction of glycogen, particularly in the periportal hepatocytes. The effects of salicylate were partially prevented by two ZnCl2 injections. The protective effects of ZnCl2 may be due to lower iron uptake into hepatocytes and by the induction of zinc metallothionein, which can serve as a scavenger for oxygen radicals.
Department of Molecular Biology and Biochemistry, Free University of Berlin, Germany.
T
T
Günther
R
R
Gossrau
J
J
Vormann
M
M
Ruhnke
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002412:Cations / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury; D005260:Female; D006651:Histocytochemistry; D007501:Iron / Q000032:analysis / Q000097:blood; D008055:Lipids / Q000032:analysis; D008107:Liver Diseases / Q000517:prevention & control*; D008112:Liver Glycogen / Q000032:analysis; D008668:Metallothionein / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012459:Salicylates / Q000032:analysis / Q000097:blood / Q000633:toxicity*; D020156:Salicylic Acid; D015032:Zinc / Q000494:pharmacology*
D002412:Cations; D008055:Lipids; D008112:Liver Glycogen; D012459:Salicylates; D008668:Metallothionein; D007501:Iron; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D015032:Zinc; D020156:Salicylic Acid