Expression and regulation of UDP-glucuronate: neolactotetraosylceramide glucuronyltransferase in the nervous system.
Sulfoglucuronyl glycolipids (SGGLs) are temporally and spatially regulated molecules present in the nervous system during its development. The characteristics of the rat brain enzyme glucuronyltransferase involved in the biosynthesis of SGGLs have been described. The enzyme catalyzes the transfer of glucuronic acid (GlcA) from UDP-GlcA to terminal galactose of the neolacto (type 2) series of glycolipids to form beta 1-3-linked glucuronyl neolacto glycolipids. The enzyme was highly specific for the neolacto series of acceptor glycolipids, neolactotetraosylceramide (nLcOse4Cer), neolactohexaosylceramide (nLcOse6-Cer), and neolactooctaosylceramide (nLcOse8Cer) and was different from the drug-inducible phenol:GlcA transferase. Considerable activity of GlcA transferase was present in the adult rat cerebral cortex, even though SGGLs almost completely disappeared from the cortex by postnatal day 15. In the cerebellum, although levels of SGGLs increased with development, the specific activity of GlcA transferase declined. The results indicated that GlcA transferase was not a regulatory enzyme controlling the expression of SGGLs. Measurements of the levels of nLcOse4Cer and nLcOse6Cer in these neural tissues indicated that the availability of these precursors may regulate the differential expression of SGGLs seen previously. GlcA transferase was significantly reduced in the cerebellar Purkinje cell degenerating murine mutant (pcd/pcd), which is consistent with the loss of SGGLs in the cerebellum of this mutant and specific association of these glycolipids with Purkinje cells.
Department of Biochemistry, Eunice Kennedy Shriver Center for Mental Retardation, Waltham, Massachusetts 02254.
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DK
Chou
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Flores
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Jungalwala
D000818:Animals; D002531:Cerebellum / Q000201:enzymology* / Q000254:growth & development; D002540:Cerebral Cortex / Q000201:enzymology*; D002855:Chromatography, Thin Layer; D003902:Detergents; D005786:Gene Expression Regulation; D005915:Globosides / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D051381:Rats; D011919:Rats, Inbred Strains; D014535:Uridine Diphosphate Glucuronic Acid / Q000235:genetics*
D003902:Detergents; D005915:Globosides; C063678:3-sulfoglucuronyl-lacto-N-neotetraosylceramide; D014535:Uridine Diphosphate Glucuronic Acid