ELISA for identification of blood meal source in black flies (Diptera: Simuliidae).
A modified ELISA, in which a biotinylated second antibody and a streptavidin-biotinylated peroxidase complex are used, has been developed to identify the blood meal sources of black flies. The modified assay is sensitive enough to identify correctly 100% of blood meals at 73 h postingestion and 80% of blood meals at 116 h postingestion in black flies held in the field at ambient temperatures.
Department of Biology, Queen's University, Kingston, Ontario, Canada.
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D000818:Animals; D000918:Antibody Specificity; D001769:Blood; D004797:Enzyme-Linked Immunosorbent Assay; D005247:Feeding Behavior; D011237:Predictive Value of Tests; D012843:Simuliidae / Q000502:physiology*