Growth and cell viability of aortic versus pulmonic homografts in the systemic circulation.
Growth characteristics and cell viability of aortic and pulmonic valve homografts in the systemic circulation were compared in a growing sheep model. Seven aortic and seven pulmonic cryopreserved homografts were implanted in the descending aortae of recipient female lambs. Of seven sheep per group, three were killed at 8 months, three at 12 months, and one at 15 months. Tissue cultures were obtained on homograft valve and root wall specimens. Male donor cells were identified by chromosome analysis. Preimplant to explant changes in pulmonic homograft external diameters at the valve annulus and sinotubular junction increased significantly more than in aortic roots. The postoperative dimensions of the distal anastomotic diameter and length of the graft increased significantly more in pulmonic than in aortic roots by time of explant. Leaflet calcification and valvular stenosis did not develop in either pulmonic or aortic homografts over the period of observation. Calcification in the root wall was significantly less in pulmonic than aortic homografts. Growth on tissue culture was obtained from over 70% of homograft specimens. Viable donor cells were demonstrated in none and 43% of aortic and pulmonic leaflets, respectively, and 71% and 57% of aortic and pulmonary arterial homograft walls, respectively. In conclusion, both aortic and pulmonic homograft valves provide freedom from calcification and stenosis in this model. Continuing expansion of pulmonic root diameters under systemic pressure might lead to late aneurysm formation. Although host cell repopulation of grafts may be advantageous, the presence of viable donor cells in leaflet tissue does not seem necessary to prevent calcific degeneration.
Department of Surgery, University of Washington, Seattle 98195.
M D
MD
Allen
Y
Y
Shoji
Y
Y
Fujimura
D
D
Gordon
R
R
Thomas
K G
KG
Brockbank
C M
CM
Disteche
D000818:Animals; D001013:Aorta, Thoracic; D001021:Aortic Valve / Q000254:growth & development / Q000473:pathology / Q000637:transplantation*; D002114:Calcinosis / Q000209:etiology; D002470:Cell Survival / Q000502:physiology; D005260:Female; D006085:Graft Survival / Q000502:physiology; D006439:Hemodynamics / Q000502:physiology*; D008297:Male; D011664:Pulmonary Valve / Q000254:growth & development / Q000473:pathology / Q000637:transplantation*; D012756:Sheep; D014184:Transplantation, Homologous