Isolation of functional polymorphonuclear leukocytes from rhesus monkeys using discontinuous Ficoll-Hypaque density gradients.
A simple, rapid method for separating polymorphonuclear leukocytes (PMNs) from adult rhesus monkey blood, based on the use of a discontinuous gradient of Ficoll-Hypaque (densities, 1.100, 1.077) has been developed. Using this method, 71.1% of the PMNS were recovered in a layer with a purity of 91% PMNs with very few contaminating erythrocytes. The added advantage of the method described here is that a higher chemotactic activity was retained.
Taniuchi
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Department of Pathology, School of Medicine, University of California, Davis.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002499:Centrifugation, Density Gradient; D002634:Chemotaxis, Leukocyte; D008253:Macaca mulatta / Q000097:blood*; D009504:Neutrophils