Very early detection of changes associated with cellular activation using a modified flow cytometer.
A sample station modification previously described has been redesigned to provide greater flexibility and enhanced performance. The improved modification provides mixing and temperature regulation in a compact unit that mounts close to the nozzle holder for reduced transit times, allowing for addition of mediators to a sample in place on the flow cytometer, with observation of results in approximately 1 s. An electronic circuit activated at the time of injection generates full-scale pulses in the forward scatter channel. This provides the data with a time stamp for direct correlation of injection and cellular response. A detailed description of the modification, performance verification data, and practical applications in the measurements associated with cellular activation are presented.
Institute for Arthritis and Autoimmunity, Miles Research Center, West Haven, Connecticut 06516.
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Kelley
D002118:Calcium / Q000378:metabolism; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials / Q000502:physiology; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D010084:Oxidation-Reduction
D002118:Calcium