Use of nitrogen-15 determinations of purine nitrogen fraction of digesta to define nitrogen metabolism traits in the rumen.
A method for direct purine N isolation and determination was modified from the method of Zinn and Owens for rapid determination of purines. By this method, N derived from purines in digesta was measured and collected for 15N determinations. Measurements of purine N in 33 samples of ruminal contents and duodenal digesta of sheep and goats were compared with purine determinations in the same samples. The results showed that essentially all the N in the purines that could be isolated by the Zinn and Owens procedure also could be detected by N isolation and determination. Determinations of 15N in the NH3 N, NAN, and purine N fractions in a continuous culture in vitro showed significant differences in the 15N enrichment curves between diets based on either roasted or raw soybean meal.
Department of Animal Science, Faculty of Agriculture Rehovot, Israel.
Y
Y
Aharoni
H
H
Tagari
D000818:Animals; D002417:Cattle / Q000502:physiology*; D004044:Dietary Proteins / Q000378:metabolism*; D006358:Hot Temperature; D007553:Isotope Labeling; D009584:Nitrogen / Q000378:metabolism*; D009587:Nitrogen Isotopes; D011687:Purines / Q000378:metabolism*; D012044:Regression Analysis; D012417:Rumen / Q000502:physiology*; D013025:Glycine max
D004044:Dietary Proteins; D009587:Nitrogen Isotopes; D011687:Purines; D009584:Nitrogen