Human synovial mast cell involvement in rheumatoid arthritis and osteoarthritis. Relationship to disease type, clinical activity, and antirheumatic therapy.
Mast cells were isolated by enzymatic digestion of synovium obtained from 48 patients with rheumatoid arthritis (RA) and 42 patients with osteoarthritis (OA). A significantly lower percentage of stainable synovial mast cells was obtained by tissue digestion from patients with clinically active RA compared with those with less active disease. The 54 patients treated with nonsteroidal antiinflammatory drugs had a significantly lower percentage of stainable synovial mast cells in cell suspension than did the other 36 patients. When anti-IgE antibody was used as a secretagogue in vitro, significantly greater histamine release was observed from synovial mast cells of RA patients compared with OA patients. Greater histamine release in response to anti-IgE was observed in the RA patients with more clinically active disease and those who were treated with prednisone, compared with RA patients without these features. Synovial mast cells of RA patients treated with a disease-modifying antirheumatic drug had a significantly lower mean histamine content than did cells from patients not receiving such treatment. Our data suggest that there are differences between synovial mast cells from tissues of patients with RA and OA and suggest that synovial mast cells may be activated in clinically active RA. In addition, the data indicate an effect of systemic antirheumatic therapy on mast cells isolated from synovium of patients with arthritis.
Department of Medicine, University of Wisconsin Hospitals and Clinics, Madison.
A J
AJ
Bridges
D G
DG
Malone
J
J
Jicinsky
M
M
Chen
P
P
Ory
W
W
Engber
F M
FM
Graziano
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D001172:Arthritis, Rheumatoid / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology; D002452:Cell Count; D006632:Histamine / Q000378:metabolism; D006801:Humans; D008407:Mast Cells / Q000378:metabolism / Q000473:pathology* / Q000502:physiology; D008875:Middle Aged; D010003:Osteoarthritis / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology; D011241:Prednisone / Q000627:therapeutic use; D013583:Synovial Membrane / Q000473:pathology*
D000893:Anti-Inflammatory Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D006632:Histamine; D011241:Prednisone