Isolation of strains of Thermus aquaticus from the Australian Artesian Basin and a simple and rapid procedure for the preparation of their plasmids.
Thirty four Thermus aquaticus strains have been isolated from the non-volcanically naturally heated waters of the Australian Artesian Basin which extends the known ecological habitat of this group of organisms. A simple and rapid method developed for isolation of plasmids indicated that considerable variation in numbers and molecular sizes existed within the 23 strains that were investigated. Dissimilar plasmid profiles were obtained from the strains that been isolated from the source waters and those that had been isolated from the runoff channels formed by these source waters.
Denman
S
S
Division of Science and Technology, Griffith University, Nathan, Brisbane, Australia.
Hampson
K
K
Patel
B K
BK
D001315:Australia; D004463:Ecology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D010957:Plasmids; D013824:Thermus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D014871:Water Microbiology