Reperfusion of ischemic skeletal muscle causes erythrocyte hemolysis and decreases subsequent oxidant-mediated lung injury.
A prior transient hindlimb ischemia/reperfusion (I/R) insult decreased acute lung injury in rats subsequently treated with cobra venom factor. I/R-mediated protection was associated with erythrocyte hemolysis, increased plasma catalase activity, and increased plasma hydrogen peroxide scavenging activity. In contrast, hindlimb I/R did not increase lung catalase activity, and large amounts of injected catalase were required to increase lung catalase activity. The results suggest that limited I/R in one organ can induce systemic processes that may decrease a subsequent O2 metabolite-mediated injury in another distant organ. The mechanism may involve release of catalase from hemolyzed erythrocytes at levels that are not sufficient to measurably increase total lung catalase activity.
Webb-Waring Lung Institute, University of Colorado Health Sciences Center, Denver 80262.
J A
JA
Leff
D A
DA
Kennedy
L S
LS
Terada
M
M
Emmett
H J
HJ
McCutchan
D L
DL
Walden
J E
JE
Repine
D000818:Animals; D002374:Catalase / Q000032:analysis / Q000097:blood; D004546:Elapid Venoms / Q000494:pharmacology; D004912:Erythrocytes / Q000473:pathology / Q000502:physiology*; D006461:Hemolysis / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000097:blood; D008168:Lung / Q000737:chemistry / Q000201:enzymology; D055370:Lung Injury; D008297:Male; D009132:Muscles / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D016877:Oxidants / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism; D011654:Pulmonary Edema / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000150:complications / Q000473:pathology / Q000503:physiopathology*
D004546:Elapid Venoms; D016877:Oxidants; C016310:cobra venom factor; D006861:Hydrogen Peroxide; D002374:Catalase; D010100:Oxygen