[Relationship among glycated compounds, superoxide dismutase activities, and other related analytes in diabetic patients classified by ages].
Among several glycated compounds (GC) which are based on Maillard reaction, glycated hemoglobin (HbA1c) and fructosamine (FRA) have been utilized widely as a markers of diabetes. Recently, glycated albumin (GA) has been pointed out as a new indicator. For the determination of GA, spectrophotometry combined with an affinity column method has been mainly used, however the procedure is complicated. Recently a two-column HPLC method (ion-exchange column and affinity column) has been developed by Shima. We have evaluated a GA analyzer GAA-2000 based on Shima's method. After a series of fundamental and performance evaluation studies, the GAA-2000 was found to be appropriate for our study. Reference values obtained from this equipment were 10.56-16.87%. Correlation coefficients based on GA using diabetic and diabetic nephropathy patient specimens (n = 87) were: FRA (r = 0.944) greater than HbA1c (r = 0.842) greater than Glucose (r = 0.510) Superoxide dismutase (SOD) and lipid peroxidase (LPO) with are produced in relation to active oxygen did not show a good correlation. Although we tried classify the patients according to juvenile (20-39), middle (40-64) and senile (greater than 65) the method of Asada et al., we could not find any distinct tendencies.
Department of Central Laboratory Mitsui Memorial Hospital.
E
E
Maehata
H
H
Shimomura
H
H
Kiyose
A
A
Hayashi
Y
Y
Sakagishi
D000368:Aged; D000375:Aging / Q000378:metabolism*; D003928:Diabetic Nephropathies / Q000097:blood* / Q000201:enzymology; D019270:Fructosamine; D006442:Glycated Hemoglobin / Q000032:analysis; D017127:Glycation End Products, Advanced; D006595:Hexosamines / Q000097:blood; D006801:Humans; D012709:Serum Albumin / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*; D000093423:Glycated Serum Albumin
D019270:Fructosamine; D006442:Glycated Hemoglobin; D017127:Glycation End Products, Advanced; D006595:Hexosamines; D012709:Serum Albumin; D013482:Superoxide Dismutase; D000093423:Glycated Serum Albumin