Location and sequence of the todF gene encoding 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase in Pseudomonas putida F1.
The gene (todF) encoding 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase in Pseudomonas putida F1 was shown to be located upstream of the todC1C2BADE genes. The latter form part of the tod operon and encode the enzymes responsible for the initial reactions in toluene degradation. The nucleotide (nt) sequence of todF was determined and the deduced amino acid (aa) sequence revealed that the hydrolase contains 276 aa with a Mr of 30,753. The deduced aa sequence was 63.5% homologous to that reported for 2-hydroxymuconic semialdehyde hydrolase which is involved in phenol degradation by Pseudomonas CF600.
Menn
F M
FM
Department of Microbiology, University of Iowa, Iowa City 52242.
Zylstra
G J
GJ
Gibson
D T
DT
D000595:Amino Acid Sequence; D001483:Base Sequence; D005798:Genes, Bacterial; D006867:Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009876:Operon; D016958:Pseudomonas putida / Q000201:enzymology / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D006867:Hydrolases; C021416:2-hydroxy-6-oxo-2,4-heptadienoate hydrolase