Isolation of a human placenta cDNA coding for a protein related to the vascular permeability factor.
A human cDNA coding for a protein related to the vascular permeability factor (VPF) was isolated from a term placenta cDNA library; we therefore named its product placenta growth factor (PlGF). PlGF is a 149-amino-acid-long protein and is highly homologous (53% identity) to the platelet-derived growth factor-like region of human VPF. Computer analyses reveal a putative signal peptide and two probable N-glycosylation sites in the PlGF protein, one of which is also conserved in human VPF. By using N-glycosidase F, tunicamycin, and specific antibodies produced in both chicken and rabbit, we demonstrate that PlGF, derived from transfected COS-1 cells, is actually N-glycosylated and secreted into the medium. In addition, PlGF, like VPF, proves to be a dimeric protein. Finally, a conditioned medium from COS-1 cells containing PlGF is capable of stimulating specifically the growth of CPA, a line of endothelial cells, in vitro.
Maglione
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International Institute of Genetics and Biophysics, Consiglio Nazionale della Ricerce, Naples, Italy.
Guerriero
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Viglietto
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Delli-Bovi
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Persico
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MG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002822:Choriocarcinoma; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D016228:Endothelial Growth Factors / Q000235:genetics*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D005260:Female; D015723:Gene Library; D006801:Humans; D008222:Lymphokines / Q000235:genetics*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D010920:Placenta / Q000502:physiology*; D000072483:Placenta Growth Factor; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis / Q000235:genetics* / Q000494:pharmacology; D011994:Recombinant Proteins / Q000032:analysis / Q000494:pharmacology; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D014594:Uterine Neoplasms; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D008222:Lymphokines; D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; C000608036:PGF protein, human; D011257:Pregnancy Proteins; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D000072483:Placenta Growth Factor; D004247:DNA