Ethanol improves the transformation efficiency of intact yeast cells.
A technique is described in which ethanol is used to improve the genetic transformation of intact yeast (Saccharomyces cerevisiae) cells pretreated with LiAc and PEG. Transformation efficiency was increased with increasing concentrations of ethanol with a peak at 10% concentration. The effect varies with different yeast strains and plasmids and up to a maximum of a 15-fold increase was observed.
Department of Microbiology, Charles University, Vinicna, Prague, Czechoslovakia.
V
V
Lauermann
D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D000431:Ethanol / Q000494:pharmacology*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D014170:Transformation, Genetic / Q000187:drug effects*
D000431:Ethanol; D004247:DNA