Comparison of preservation methods for enterotoxigenic Escherichia coli producing heat-labile enterotoxin.
Ten strains of enterotoxigenic Escherichia coli producing heat-labile enterotoxin (LT) were preserved under 12 different conditions. After 1 month, 9 months, and 3 years of preservation, the cultures were recovered and examined for LT production. Preservation of the cultures on Dorset Egg Medium at 4 degrees C and preservation by freezing the cell suspensions in tryptic soy broth with 20% glycerol were found to be suitable preservation methods; all strains were alive for 3 years and had a minimum loss of LT production.
Yoh
M
M
Laboratory for Culture Collection, Osaka University, Japan.
Narita
I
I
Honda
T
T
Miwatani
T
T
Nishibuchi
M
M
D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics; D001431:Bacteriological Techniques; D015925:Cryopreservation; D003470:Culture Media; D004768:Enterotoxins / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005069:Evaluation Studies as Topic; D006801:Humans; D010957:Plasmids; D011309:Preservation, Biological / Q000379:methods*
D001427:Bacterial Toxins; D003470:Culture Media; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli