ADP/ATP translocator is essential only for anaerobic growth of yeast Saccharomyces cerevisiae.
All three genes (AAC1, AAC2 and AAC3) encoding the mitochondrial ADP/ATP translocator, were inactivated in a haploid yeast strain by a gene disruption technique. The triple mutant was still able to grow on fermentable carbon sources but only in the presence of oxygen. Under aerobic conditions neither translocator-protein nor carrier-mediated transport was detected in all mutants in which the AAC2 gene was disrupted. It was further shown that a functional AAC genes product is essential only for anaerobic growth of Saccharomyces cerevisiae but not for growth under derepressed conditions. Under anaerobic conditions a non-detectable amount of AAC3 gene product is sufficient to ensure the cell growth and multiplication.
Cancer Research Institute, Slovak Academy of Sciences, Bratislava.
T
T
Drgon
L
L
Sabová
N
N
Nelson
J
J
Kolarov
D000244:Adenosine Diphosphate / Q000378:metabolism; D000693:Anaerobiosis; D005800:Genes, Fungal; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology; D000226:Mitochondrial ADP, ATP Translocases / Q000235:genetics / Q000378:metabolism*; D016364:Reading Frames; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*
D000244:Adenosine Diphosphate; D000226:Mitochondrial ADP, ATP Translocases