Growth hormone-releasing factor stimulates calcium entry in the GH3 pituitary cell line.
The GH3 pituitary cell line has been extensively used to study various aspects of the stimulus secretion coupling process. It is known that GH3 cells release PRL and GH in the basal state and in response to various secretagogues. However, this cell line was considered unsuitable as a model for studying the effects of GHRF since the neuropeptide did not affect GH secretion or gene expression. This suggested that the GH3 cells may lack GHRF receptors. The present study investigates the effect of GHRF on free intracellular Ca2+ concentrations in GH3 cells. Cytosolic free calcium concentrations ([Ca2+]i) were monitored in individual cells by microspectrofluorimetry using the fluorescent dye indo 1. When the cells were challenged with a brief application of GHRF (100 nM; 15 sec), 36 out of 59 of these cells responded within a few seconds by a marked increase in [Ca2+]i. GHRF enhanced the frequency of [Ca2+]i oscillations in spontaneously active cells or triggered [Ca2+]i oscillations in inactive cells. The response to GHRF was totally blocked by external Ca2+ free solutions and Ca2+ channel blockers. Combined electrophysiological and fluorescent experiments were carried out in 16 cells. Eleven responded to GHRF. In all cases, the Ca2+ transients triggered by GHRF were associated with action potentials. The Ca2+ responses observed in our experiments clearly show that GH3 cells possess membrane receptors to GHRF. Thus, it is likely that the lack of secretory response observed in GH3 cells does not result from the absence of binding sites to the peptide. It is more likely to be related to alterations of transduction mechanisms resulting in uncoupling between stimulation and secretion.
Bresson
L
L
CNRS URA 1200, Laboratoire de Neurophysiologie, Université de Bordeaux II, France.
Fahmi
M
M
Sartor
P
P
Dufy
B
B
Dufy-Barbe
L
L
D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D004594:Electrophysiology; D013007:Growth Hormone-Releasing Hormone / Q000494:pharmacology*; D009994:Osmolar Concentration; D010902:Pituitary Gland / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D013050:Spectrometry, Fluorescence
D013007:Growth Hormone-Releasing Hormone; D002118:Calcium