[Evaluation of a new immunoturbidimetry technique for measuring microalbuminuria].
Subclinical elevation of urinary albumin excretion is a good predictor of later clinical proteinuria. A simple, sensitive and rapid immunoturbidimetric method was developed to quantify urinary albumin excretion (URIN-PAK ImmunoMICRO LAB, Miles Italia Spa). In the presence of polyethylene glycol 6000, immunocomplex between human albumin and its specific antibody are rapidly formed (5-50 min, at room temperature). Absorbance reading are mode U 340 nm (Automatic Analyzer RA 1000, Technicon). The test is specific for albumin failing to cross react with other plasma proteins present in urine, as well as with glibenclamide, chlorpropamide, phenformin, hemoglobin, glucose, urea and thymol. The present method correlates with SCLAVO H-ALBUMIN RIA Kit (r = 0.9917). The test is suitable for clinical use.
I Divisione Medicina Ospedale S. Martino, Genova.
G
G
Gigli
F
F
Altomonte
B
B
Bocca
M
M
Colombano
R
R
De Grandi
M
M
Ponte
A
A
Triolo
D000419:Albuminuria / Q000175:diagnosis / Q000453:epidemiology / Q000209:etiology / Q000652:urine*; D000936:Antigen-Antibody Complex / Q000652:urine; D003922:Diabetes Mellitus, Type 1 / Q000150:complications / Q000652:urine; D003928:Diabetic Nephropathies / Q000175:diagnosis / Q000453:epidemiology / Q000652:urine*; D006801:Humans; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D011233:Precipitin Tests; D011237:Predictive Value of Tests; D015995:Prevalence; D011863:Radioimmunoassay / Q000379:methods; D011933:Reagent Kits, Diagnostic; D014556:Urine / Q000737:chemistry
D000936:Antigen-Antibody Complex; D011933:Reagent Kits, Diagnostic
Valutazione di una nuova tecnica immunoturbidimetrica per il dosaggio della microalbuminuria.