cDNA sequence of a Drosophila melanogaster gene, Dfur1, encoding a protein structurally related to the subtilisin-like proprotein processing enzyme furin.
Screening a genomic library of Drosophila melanogaster DNA with a human fur cDNA probe resulted in the isolation of DNA clones that apparently belonged to two different DNA regions of the Drosophila genome. Subsequently, corresponding Drosophila cDNA clones were isolated. Nucleotide sequence analysis indicated that these cDNA clones originated from two different genes, which were called Dfur1 and Dfur2. From overlapping Dfur1 cDNA clones, a composite cDNA could be constructed and analysis of its nucleotide sequence revealed the coding sequence for a protein of 899 amino acid residues. This protein, designated Dfurin1, exhibited striking sequence homology to human furin and contained the same protein domains except for the cysteine-rich region. Furthermore, unlike human furin, Dfurin1 possessed an extended amino-terminal region in which a potential transmembrane anchor was present.
Molecular Oncology Section, University of Leuven, Belgium.
A J
AJ
Roebroek
I G
IG
Pauli
Y
Y
Zhang
W J
WJ
van de Ven
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D045683:Furin; D015698:Genomic Library; D006801:Humans; D007447:Invertebrate Hormones / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013381:Subtilisins / Q000235:genetics*
D029721:Drosophila Proteins; D007447:Invertebrate Hormones; D004247:DNA; D011505:Protein-Tyrosine Kinases; D013381:Subtilisins; C079637:Fur1 protein, Drosophila; D045683:Furin