Antibody-dependent enhancement and neutralization pattern of sera from SIV-infected or HIV-2-vaccinated rhesus monkeys.
Neutralizing and enhancing activities in sera were detected by using an in vitro infection assay of HUT78 cells. Ten animals were vaccinated with HIV-2ROD recombinant vaccinia viruses and respective purified proteins. Only sera from monkeys vaccinated with env elicited neutralizing antibodies. No antibody-dependent enhancement (ADE) properties were detected in all the tested sera. Six other macaques were infected with SIV-mac251. All of them had detectable ADE properties in their sera. No major neutralizing activity was detected.
Département de Toxicologie Expérimentale, Centre de Recherches du Service de Santé des Armées, Fontenay aux Roses, France.
R
R
Le Grand
G
G
Vogt
A
A
Chapel
D
D
Dormont
D016915:AIDS Vaccines / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005500:Follow-Up Studies; D015483:HIV Antibodies / Q000097:blood; D015498:HIV-2 / Q000276:immunology*; D008253:Macaca mulatta; D009500:Neutralization Tests; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary; D014614:Vaccines, Synthetic / Q000276:immunology
D016915:AIDS Vaccines; D000914:Antibodies, Viral; D015483:HIV Antibodies; D014614:Vaccines, Synthetic