Chemiluminescence assay of N-acetyl-beta-D-glucosaminidase in urine using o-aminophthalylhydrazido-N-acetyl-beta-D-glucosaminide.
The chemiluminescence assay of urinary N-acetyl-beta-D-glucosaminidase using o-aminophthalylhydrazido-N-acetyl-beta-D-glucosaminide as the substrate was examined. Under optimized conditions, a linear chemiluminescence response to the standard N-acetyl-beta-D-glucosaminidase (NAGase) of different concentrations was observed with a correlation coefficient of 0.999 over the range of 0.3 to 20 I.U./l. The present method was compared with the prevalent method using p-nitrophenyl-N-acetyl-beta-D-glucosaminide in the assay of 20 urine samples from healthy subjects, and the correlation coefficient of 0.891 was obtained.
Dojindo Laboratories, Kumamoto, Japan.
K
K
Sasamoto
R
R
Zenko
K
K
Ueno
Y
Y
Ohkura
D000117:Acetylglucosamine / Q000031:analogs & derivatives*; D000118:Acetylglucosaminidase / Q000652:urine*; D000328:Adult; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol / Q000031:analogs & derivatives*
C065472:3-aminophthalylhydrazido-N-acetyl-beta-glucosaminide; D008165:Luminol; D000118:Acetylglucosaminidase; D000117:Acetylglucosamine