Cloning and chromosomal mapping of nuclear genes encoding chloroplast and cytosolic glyceraldehyde-3-phosphate-dehydrogenase from Arabidopsis thaliana.
Both cDNA and genomic clones for the nuclear genes encoding chloroplast (cp) (gapA and gapB) and cytosolic (gapC) glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Arabidopsis thaliana have been isolated and characterized. Genomic Southern-blot analyses indicate that there is only one copy of each gapA, gapB and gapC gene in A. thaliana. Comparison of the deduced amino acid (aa) sequences shows that the A and B subunits are highly similar (80% positional aa identity), while there is less similarity between the cp and cytosolic subunits (45% aa identity). These relationships are consistent with the idea that the cp and cytosolic GAPDHs evolved from different lineages, as suggested in our previous study of tobacco GAPDHs [Shih et al., Cell 47 (1986) 73-80]. In addition, the chromosomal locations for the three gap genes were determined by restriction fragment length polymorphism mapping; the three gap genes are not closely linked, gapA (55.8 cM) and gapC (0.0 cM) are on chromosome 3, and gapB (51.3 cM) is on chromosome 1.
Shih
M C
MC
Department of Genetics, Harvard Medical School, Massachusetts General Hospital, Boston 02114.
Heinrich
P
P
Goodman
H M
HM
D000595:Amino Acid Sequence; D001483:Base Sequence; D002467:Cell Nucleus; D002736:Chloroplasts / Q000378:metabolism*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003600:Cytosol / Q000378:metabolism*; D004247:DNA; D015698:Genomic Library; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics* / Q000378:metabolism; D010944:Plants / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D010446:Peptide Fragments; C068142:glyceraldehyde 3-phosphate dehydrogenase (304-313); D004247:DNA; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases