Calcitonin gene-related peptide increases the production of glycosaminoglycans but not of collagen type I and III in cultures of rat fat-storing cells.
We investigated whether calcitonin gene-related peptide (CGRP) was able to affect the production of collagen and glycosaminoglycans (GAG) in cultures of rat fat-storing cells (FSC). Rat CGRP (1 nM-1 microM) induced a dose-dependent increase of total GAG production in FSC cultures with an EC50 of 28 nM. One uM human CGRP (8-37) shifted the dose-response curve of rat CGRP to the right (EC50 = 257 nM) without depressing the maximal response. Salmon calcitonin (1 nM-1 microM) did not significantly modify total GAG accumulation in FSC cultures. Collagen type I and III production was not significantly affected by either CGRP or calcitonin in FSC cultures. These findings suggest that peripheral sensory neuropeptides may modulate liver fibrogenesis.
Dip. Fisiopatologia Clinica, Università di Firenze, Italy.
A
A
Casini
G
G
Galli
R
R
Salzano
P
P
Santicioli
C A
CA
Maggi
C M
CM
Rotella
C
C
Surrenti
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002116:Calcitonin / Q000494:pharmacology; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D005260:Female; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D006025:Glycosaminoglycans; C028815:salmon calcitonin; D002116:Calcitonin; D003094:Collagen; D015740:Calcitonin Gene-Related Peptide