Quantitative determination of different apolipoprotein B containing lipoproteins by an enzyme linked immunosorbent assay: apo B with apo C-III and apo B with apo E.
A non competitive enzyme-linked immunosorbent assay (ELISA) for total apolipoprotein (apo) B, apo B with apo C-III (LpC-III:B), and apo B with apo E (LpE:B), was developed. Microtiter plates were used as solid-phase, and subdivided into three parts, coated respectively with affinity purified antibodies to apo B, to apo C-III and to apo E. After incubating the antigen with coated plates, a horseradish peroxidase-labelled antibody to apo B was added to all the plates to estimate total apo B, LpC-III:B and LpE:B.
U 279 et U 325, Institut Pasteur, Lille.
A
A
Kandoussi
C
C
Cachera
D
D
Parsy
J M
JM
Bard
J C
JC
Fruchart
D053305:Apolipoprotein C-III; D001055:Apolipoproteins B / Q000032:analysis*; D001056:Apolipoproteins C / Q000032:analysis*; D001057:Apolipoproteins E / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood*; D008074:Lipoproteins / Q000097:blood* / Q000302:isolation & purification; D012680:Sensitivity and Specificity
D053305:Apolipoprotein C-III; D001055:Apolipoproteins B; D001056:Apolipoproteins C; D001057:Apolipoproteins E; D008074:Lipoproteins