Crystal structure of recombinant rabbit interferon-gamma at 2.7-A resolution.
The crystal structure of recombinant rabbit interferon-gamma was solved by the multiple isomorphous replacement technique at 2.7-A resolution and refined to a crystallographic R-factor of 26.2%. The interferon crystallizes with one-half of the functional dimer in the asymmetric unit, with the two polypeptide chains of the dimer related by a crystallographic 2-fold symmetry axis. The structure is predominantly alpha-helical with extensive interdigitation of the alpha-helical segments of the two polypeptide chains.
National Cancer Institute-FCRDC, ABL-Basic Research Program, Frederick, Maryland 21702.
C T
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Samudzi
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Burton
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Rubin
D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D007371:Interferon-gamma / Q000737:chemistry* / Q000235:genetics; D046911:Macromolecular Substances; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011817:Rabbits; D011994:Recombinant Proteins; D012689:Sequence Homology, Nucleic Acid; D012997:Solvents; D014961:X-Ray Diffraction / Q000379:methods
D046911:Macromolecular Substances; D011994:Recombinant Proteins; D012997:Solvents; D007371:Interferon-gamma