Serial propagation of porcine enteric calicivirus in a continuous cell line. Effect of medium supplementation with intestinal contents or enzymes.
A porcine enteric calicivirus (PEC) was adapted to serial propagation in a continuous porcine kidney cell line (LLC-PK) by inclusion of an intestinal contents preparation (ICP), derived from uninfected gnotobiotic pigs, in the maintenance medium. Infected cells were detected by immunofluorescent staining of cell monolayers. Attempts to propagate PEC in the presence of various intestinal enzymes of porcine origin, bacterial protease, bovine ICP, avian ICP or without medium supplementation were unsuccessful.
Ohio Agricultural Research and Development Center, Ohio State University, Wooster.
A V
AV
Parwani
W T
WT
Flynn
K L
KL
Gadfield
L J
LJ
Saif
D000818:Animals; D002139:Caliciviridae / Q000254:growth & development*; D002460:Cell Line; D003470:Culture Media; D005766:Gastrointestinal Contents; D007422:Intestines / Q000201:enzymology; D007668:Kidney / Q000166:cytology / Q000382:microbiology; D012692:Serial Passage; D013552:Swine / Q000382:microbiology; D014776:Virus Cultivation
D003470:Culture Media