Inhibitory effect of 11 beta-hydroxypregna-1,4-diene-3,20-dione (delta HOP) on lymphocyte proliferation.
We have compared the immunosuppressive effect of delta HOP and glucocorticoids on lymphocyte proliferation and IL-1 secretion. The new synthetic steroid only inhibited proliferation of phytohaemagglutinin (PHA)-stimulated human lymphocyte at high concentrations and the effect did not persist after washing out the steroid. In contrast, glucocorticoids produced the classical dose-response inhibition and the effect persisted when they were removed from the cultured medium. Although both steroids decreased IL-1 secretion from human monocytes stimulated with lipopolysaccharide (LPS), they exert the same effect through a different mechanism. The experiments we report suggest that the decrease of IL-1 synthesis produced by delta HOP could be caused by inhibition of LPS phagocytosis. These results support our hypothesis that delta HOP exerts its immunosuppressive and anti-inflammatory effect by a non-genomic mechanism.
Instituto de Biologia y Medicina Experimental, Buenos Aires, Argentina.
C M
CM
Rondinone
R
R
Schillaci
M B
MB
Castillo
A
A
Roldán
D000328:Adult; D000818:Animals; D002176:Candida albicans / Q000276:immunology; D005938:Glucocorticoids / Q000494:pharmacology; D006801:Humans; D006908:Hydroxyprogesterones / Q000494:pharmacology*; D007166:Immunosuppressive Agents; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D008264:Macrophages / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats
D005938:Glucocorticoids; D006908:Hydroxyprogesterones; D007166:Immunosuppressive Agents; D007375:Interleukin-1; C038404:pregna-1,4-diene-11-ol-3,20-dione