Convenient agarose medium for simultaneous determination of the low-calcium response and Congo red binding by virulent strains of Yersinia enterocolitica.
A simple, efficient method for identification and differentiation of Yersinia enterocolitica containing virulent plasmid-bearing clones is described. The method is based on temperature of incubation and the low calcium response of the organism in an agarose Congo red medium.
U.S. Department of Agriculture, Agricultural Research Service, Philadelphia, Pennsylvania 19118.
S
S
Bhaduri
C
C
Turner-Jones
R V
RV
Lachica
D002118:Calcium / Q000494:pharmacology; D003224:Congo Red; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D010957:Plasmids; D012703:Serotyping; D014774:Virulence / Q000235:genetics; D015008:Yersinia enterocolitica / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*
D004269:DNA, Bacterial; D003224:Congo Red; D002118:Calcium