Does trans-lesion synthesis explain the UV-radiation resistance of DNA synthesis in C. elegans embryos?
Over 10-fold larger fluences were required to inhibit both DNA synthesis and cell division in wild-type C. elegans embryos as compared with other model systems or C. elegans rad mutants. In addition, unlike in other organisms, the molecular weight of daughter DNA strands was reduced only after large, superlethal fluences. The molecular weight of nascent DNA fragments exceeded the interdimer distance by up to 19-fold, indicating that C. elegans embryos can replicate through non-instructional lesions. This putative trans-lesion synthetic capability may explain the refractory nature of UV radiation on embryonic DNA synthesis and nuclear division in C. elegans.
Department of Biology, Texas Christian University, Fort Worth 76129.
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P
Hartman
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J
Reddy
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BA
Svendsen
D000818:Animals; D002107:Caenorhabditis / Q000196:embryology / Q000235:genetics* / Q000528:radiation effects; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis* / Q000528:radiation effects; D004249:DNA Damage; D008856:Microscopy, Fluorescence; D009154:Mutation; D014466:Ultraviolet Rays
D004247:DNA