The plasminogen activator family from the salivary gland of the vampire bat Desmodus rotundus: cloning and expression.
Complementary DNAs coding for four Desmodus rotundus salivary plasminogen activators (DSPAs) were isolated and characterized. The predicted amino acid sequences display structural features also found in tissue-type plasminogen activator. The largest forms (DSPA alpha 1 and -alpha 2) contain a signal peptide, a finger (F), an epidermal growth factor (EGF), a kringle, and a serine protease domain, whereas DSPA beta and -gamma lack the F and F-EGF domains, respectively. Additional differences between the four forms suggest that distinct genes code for the members of the DSPA family. Transfection of DSPA-encoding cDNAs, placed under the control of the simian virus 40 late promoter, into COS-1 cells resulted in the secretion of highly fibrin-dependent PAs.
Research Laboratories of Schering AG Berlin, F.R.G.
J
J
Krätzschmar
B
B
Haendler
G
G
Langer
W
W
Boidol
P
P
Bringmann
A
A
Alagon
P
P
Donner
W D
WD
Schleuning
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D002685:Chiroptera; D003001:Cloning, Molecular; D004247:DNA; D008969:Molecular Sequence Data; D010960:Plasminogen Activators / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011487:Protein Conformation; D012469:Salivary Glands / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid
D004247:DNA; D010960:Plasminogen Activators