Isoelectric focusing in immobilized pH gradients: applications in clinical chemistry and forensic analysis.
The applications of isoelectric focusing in immobilized pH gradients in clinical chemistry and forensic analysis are reviewed. Strong emphasis is given to the separation of serum proteins, in particular alpha 1-acidic glycoprotein, acid phosphatase, alkaline phosphatase, alpha 1-antitrypsin, apolipoproteins, complement component, factor B, factor XIIIB, group-specific component, lecithin:cholesterol acyltransferase, phosphoglucomutase, prealbumin, protein C and transferrin. The analysis of human parotid salivary proteins is discussed and an assessment is given of the state of the art in thalassaemia screening.
Department of Biomedical Sciences and Technologies, University of Milan, Italy.
P G
PG
Righetti
E
E
Gianazza
A
A
Bianchi-Bosisio
P
P
Sinha
E
E
Köttgen
D001798:Blood Proteins / Q000302:isolation & purification; D002624:Chemistry, Clinical / Q000379:methods*; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods; D011296:Prenatal Diagnosis / Q000379:methods; D013789:Thalassemia / Q000175:diagnosis
D001798:Blood Proteins