[Erythroblast antigen expression in murine hematopoietic and nonhematopoietic cells. 2. An immunofluorescent study in pre- and postnatal ontogeny using monoclonal antibodies].
The expression of erythroblast antigen (Ag-Eb) in cell membranes during pre- and postnatal mouse development was studied by immunofluorescence using the monoclonal antibody MAE-15. Ag-Eb was detected in embryonic liver, spleen, epithelia of intestine, various glands and skin, as well as in extraembryonic tissues (yolk sac and trophoblast). In pregnant mice positive immunofluorescence was observed in placenta and on the surface of decidual cells in uterus. In adult non-pregnant mice Ag-Eb expression was detected not only in membranes of erythroid cells, but also in non-hemopoietic tissues, such as epithelia of various glands, intestine, kidney and testis, brain endothelium, basal layer of epidermis, and intercalated discs of the heart muscle. A possible role of Ag-Eb in processes of cell transport is discussed.
Shipova
L Ia
LIa
Mechetner
E B
EB
Rozinova
E N
EN
Tonevitskiĭ
A G
AG
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D004622:Embryo, Mammalian; D004900:Erythroblasts / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D005865:Gestational Age; D006128:Growth / Q000276:immunology*; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D051379:Mice; D011247:Pregnancy
D000911:Antibodies, Monoclonal; D000941:Antigens
Ekspressiia antigena éritroblastov v krovetvornykh i nekrovetvornykh kletkakh myshi. 2. Immunofluorestsentnoe issledovanie v pre- i postnatal'nom ontogeneze s pomoshch'iu monoklonal'nykh antitel.