Kinetics of equilibration of radioiodide in individual mouse thyroid follicles in vivo.
Microdensitometric measurements were made on autoradiographs of radioiodide localized in mouse thyroids subjected to various degrees of stimulation, in which the formation of organic radioiodide was acutely blocked. Estimates were made of the relative concentrations of radioiodide in lumens and cells of follicles and in the nearby blood vessels. Simple models were introduced to interpret the data. Analysis of the ratio of radioiodide concentrations in the lumen and cells of follicles as a function of follicles size and time after injection indicated that smaller follicles equilibrated faster than larger follicles, that the equilibration was faster the more active the gland was, and that the release of radioiodide from follicles in the less active glands must be characterized by a time-dependent exit rate constant. Analysis of the relative concentration of luminal radioiodide as a function of follicle size at short time intervals and in the steady state indicated that the transport properties of the average epithelial cell were generally independent of follicle size.
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
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Andros
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Wollman
D000818:Animals; D001345:Autoradiography; D001692:Biological Transport; D003720:Densitometry / Q000379:methods; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D007454:Iodides / Q000097:blood / Q000493:pharmacokinetics*; D007457:Iodine Radioisotopes; D007700:Kinetics; D051379:Mice; D008809:Mice, Inbred C3H; D008954:Models, Biological; D009994:Osmolar Concentration; D013889:Thiouracil / Q000494:pharmacology; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism*
D007454:Iodides; D007457:Iodine Radioisotopes; D013889:Thiouracil