Characterization of synthetic macroporous ion-exchange resins in low-pressure cartridges and columns. Evaluation of the performance of Macro-Prep 50 S resin in the purification of anti-Klenow antibodies from goat serum.
Three ion-exchange materials and one hydrophobic-interaction chromatography packing, based on a rigid macroporous polymer with large, relatively uniform pores, have been evaluated for low-pressure liquid chromatography of antibodies. These sorbents have high capacities for both small and large proteins and are mechanically, chemically, and thermally stable. Macro-Prep 50 S. CM and Q ion-exchange materials are strongly acidic, weakly acidic, and strongly basic, respectively. Protein binding and recovery, pressure-flow properties, and chemical and thermal stability were determined for each sorbent. A rapid, two-step method for the purification of anti-Klenow antibodies from goat serum was developed, based on the Macro-Prep 50 S strong-acid cation-exchange material and the Econo-Pac HIC prepacked hydrophobic-interaction cartridge.
Bio-Rad Laboratories, CBU Research and Development, Chemical Division, Richmond, CA 94804.
L
L
Dunn
M
M
Abouelezz
L
L
Cummings
M
M
Navvab
C
C
Ordunez
C J
CJ
Siebert
K W
KW
Talmadge
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D004256:DNA Polymerase I / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000201:enzymology; D005069:Evaluation Studies as Topic; D006041:Goats / Q000276:immunology; D007475:Ion Exchange Resins / Q000737:chemistry*; D019384:Nucleic Acid Synthesis Inhibitors; D011108:Polymers / Q000032:analysis; D011485:Protein Binding
D000906:Antibodies; D007475:Ion Exchange Resins; D019384:Nucleic Acid Synthesis Inhibitors; D011108:Polymers; D004256:DNA Polymerase I