Establishment of a monoclonal antibody directed to the minor novel gangliosides in bovine brain and cultured neural cell lines.
We established a MAb N-25 reacted with a minor unknown antigen (AgX) in a commercially available GQ1b sample. It also recognized minor antigens in bovine brain (X-1, 2, 3, 4 and 5) and cultured neural cell lines (X-1). AgX is identical to X-5. X-5 is sialidase sensitive and has the common structure as X-1, which is resorcinol positive. These results suggested that novel gangliosides exist in bovine brain and neural cell lines.
Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.
H
H
Yamamoto
S
S
Tsuji
Y
Y
Nagai
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000276:immunology* / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology; D009474:Neurons / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000941:Antigens; D005732:Gangliosides; D007106:Immune Sera