[Detection of antibodies against Chlamydia in swine by an immunofluorescent test and an enzyme immunoassay].
Chlamydial IgG-antibodies were detected in 130 of 2050 porcine sera (6.3%) using an indirect immunofluorescence assay (IFA). Antibody titers varied between 1:8 and 1:16 in 87 sera (67%) and reached titers from 1:32 to 1:128 in 43 sera (33%). An ELISA closely related to the IFA-technique was developed, which confirmed the IFA-positive and IFA-negative results in 93% and 86% respectively. A significant higher percentage of positive IFA-titers greater than or equal to 1:32 was obtained from sows with abortions and infertility as well as from store pigs with pneumonia than from healthy pigs (p less than 0.5).
Wittenbrink
M M
MM
Institut für Mikrobiologie und Tierseuchen der Tierärztlichen Hochschule Hannover.
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D002690:Chlamydia Infections / Q000276:immunology / Q000662:veterinary*; D002691:Chlamydophila psittaci / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D013552:Swine; D013553:Swine Diseases / Q000276:immunology*
D000907:Antibodies, Bacterial
Nachweis von Antikörpern gegen Chlamydien beim Schwein mit Hilfe eines Immunofluoreszenz- und eines Enzymimmunotests.