Serum fluoride level and fluoride content of enameloid.
We investigated diverse groups of fish species to determine whether the fluorine (F) contents of the dental hard tissues were related to baseline serum F levels. Serum samples, enameloid, dentin, ganoid/enamel, and bone were analyzed for F by either electron microprobe or wet chemistry. Species were categorized into two groups based on the F content of the enameloid. One group contained greater than 2.6 wt% F in enameloid, whereas the other group had less than 0.45 wt% F in enameloid. The dentin and bone from all species (or, in skates, the cartilage), as well as the ganoid/enamel layer of a Holostean fish (alligator gar), showed consistently low F content. In those species whose teeth developed in sequential rows, the F content of enameloid increased with progressive tooth development. The serum F levels of all fish were below 0.05 microgram F/mL (2.63 mumol/L) and were not significantly related to the F content of the enameloid. The results substantiate the idea that F incorporation into enameloid is related to fish phylogeny, not food or habitat. It is suggested that specialized outer dental epithelial cell configurations may facilitate the incorporation of F into enameloid.
Forsyth Dental Center, Department of Electron Microscopy, Boston, Massachusetts 02115.
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D000818:Animals; D001031:Apatites / Q000032:analysis; D003743:Dental Enamel / Q000737:chemistry; D003804:Dentin / Q000737:chemistry; D004577:Electron Probe Microanalysis; D005399:Fishes / Q000033:anatomy & histology* / Q000097:blood; D005459:Fluorides / Q000032:analysis* / Q000097:blood; D010802:Phylogeny; D013057:Spectrum Analysis; D014070:Tooth / Q000737:chemistry*
D001031:Apatites; C025105:fluorapatite; D005459:Fluorides