Arachidonic acid induced increase in intracellular free calcium in guinea-pig hepatocytes.
Arachidonic acid (AA) caused elevations of intracellular free Ca2+ concentration ([Ca2+]i) measured by fura-2 fluorescence digital imaging microscopy in isolated guinea-pig hepatocytes. AA-induced increase in [Ca2+]i could still be observed when extracellular Ca2+ was chelated by EGTA, and was not influenced by either cyclooxygenase or lipoxygenase inhibitors. These results suggest that AA itself rather than its metabolites has direct effects on intracellular Ca2+ mobilization, causing the elevation of [Ca2+]i.
Department of Cardiovascular Diseases, Tokyo Medical and Dental University, Japan.
Y
Y
Hirano
S
S
Abe
T
T
Sawanobori
M
M
Hiraoka
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D016257:Fura-2; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007424:Intracellular Fluid / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D009637:Masoprocol / Q000494:pharmacology
D016718:Arachidonic Acid; D009637:Masoprocol; D000255:Adenosine Triphosphate; D002118:Calcium; D016257:Fura-2; D007213:Indomethacin