Sexing of forensic samples using PCR.
A rapid protocol has been established for sexing forensic samples by the Polymerase Chain Reaction method. Three sets of primer were used, two specific for Y chromosome repetitive sequences and one specific for X chromosome repetitive sequences. Detailed procedures of experiments, the controls and the applications to testing bloodstains and a vaginal swab are presented. The sensitivity of the test and problems due to contamination are discussed.
Pascal
O
O
Laboratoire de Biologie Moléculaire, Hotel Dieu, Centre Hospitalier Régional et Universitaire, Nantes, France.
Aubert
D
D
Gilbert
E
E
Moisan
J P
JP
D001801:Blood Stains; D004247:DNA / Q000032:analysis; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D005784:Gene Amplification; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012732:Sex Determination Analysis; D014626:Vaginal Smears; D014960:X Chromosome; D014998:Y Chromosome
D004247:DNA