Insulin-like growth factor I-like immunoreactivity in serum and tissues of the tilapia, Oreochromis mossambicus.
Tilapia serum was acidified with 0.5 M HCl and then chromatographed on an octadecasily-silica column. After washing with 4% acetic acid, the column was eluted with methanol. The eluate was evaporated to dryness. The sample cross-reacted in a human insulin-like growth factor I (IGF-I) radioimmunoassay, suggesting immunochemical similarity to human IGF-I. IGF-I-like immunoreactivity was present at high levels in tilapia liver. Other tissues containing IGF-I-like immunoreactivity included the gonad, kidney, heart, spleen, brain and muscle. The serum IGF-I-like immunoreactivity was attributed to substances with a molecular weight of 9,000 and 45,000 respectively, and it was elevated after treatment with bovine growth hormone and carp pituitary extract.
Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T.
T B
TB
Ng
T C
TC
Leung
N Y
NY
Woo
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D013006:Growth Hormone / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D008099:Liver / Q000737:chemistry; D008970:Molecular Weight; D010473:Perciformes / Q000097:blood / Q000378:metabolism*; D010902:Pituitary Gland / Q000737:chemistry; D011863:Radioimmunoassay; D014020:Tissue Extracts / Q000494:pharmacology
D014020:Tissue Extracts; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone