Detection of antibody formation in mice, rats and rabbits immunized with different Pneumocystis carinii antigens.
Antibody formation to P. carinii of human origin was determined by an indirect immunofluorescence antibody assay (IFA) after immunization of mice, rats and rabbits with pronase-treated whole cysts and soluble antigen in order to obtain more detailed data about the production of polyclonal antibodies. Antibody titre profiles over defined periods have shown that noticeable differences between the immunoreactions to whole cysts and soluble antigen occur. The soluble antigen produced an earlier and stronger titre rise (peak titres of up to 1:2560 2 to 4 weeks after immunization). In contrast to this, whole cysts produced equally high, although retarded, antibody titre peaks only in normal mice and rats and when the dose had been doubled. Nu/nu mice failed to demonstrate any reaction to these P. carinii immunogens. Cross-reactivity of the antibodies with P. carinii antigen from rat lungs was demonstrated. Possible reasons for different immunoreactions to these antigens, the importance of proteolytic digestion for the results obtained and the potential applicability of these polyclonal antibodies to a histochemical demonstration of the parasite are discussed.
Robert Koch-Institut, Berlin.
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Roth
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Janitschke
D000818:Animals; D000908:Antibodies, Fungal / Q000096:biosynthesis*; D000946:Antigens, Fungal / Q000276:immunology*; D002499:Centrifugation, Density Gradient; D003429:Cross Reactions; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007114:Immunization; D007121:Immunocompetence; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011010:Pneumocystis / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
D000908:Antibodies, Fungal; D000946:Antigens, Fungal