Differential diagnosis of cutaneous large cell lymphomas using monoclonal antibodies reactive in paraffin-embedded skin biopsy specimens.
We studied a large panel of monoclonal antibodies reactive on routinely processed paraffin-embedded tissue to determine their sensitivity and specificity in the diagnosis of 35 primary cutaneous large cell lymphomas of B- (n = 14) and T-cell origin (n = 21). The findings show that differentiation between clinically relevant subgroups can be obtained by a small panel of antibodies including L26, MB2, LN1, MT1, UCHL1, and Ber-H2. Pitfalls in the use of the reagents are discussed.
Department of Dermatology, Free University Hospital, Amsterdam, The Netherlands.
R C
RC
Beljaards
C J
CJ
Meijer
E
E
Scheffer
P
P
van der Valk
R
R
Willemze
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000032:analysis*; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000175:diagnosis / Q000276:immunology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000175:diagnosis* / Q000276:immunology; D016410:Lymphoma, T-Cell, Cutaneous / Q000175:diagnosis / Q000276:immunology; D016612:Paraffin Embedding; D010641:Phenotype; D012680:Sensitivity and Specificity; D012867:Skin / Q000276:immunology; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor