Selectable mutant phenotypes of the extremely thermophilic archaebacterium Sulfolobus acidocaldarius.
As a first step toward developing the genetic potential of extremely thermophilic archaebacteria, mutant strains of Sulfolobus acidocaldarius were selected by plating cells directly on solid medium containing one of several growth inhibitors. Three spontaneous resistance phenotypes were observed (5-fluorouracil resistance, novobiocin resistance, and L-ethionine resistance), each at a different average frequency. Characterization of representative strains showed each of the three mutant phenotypes to provide a potentially useful genetic marker.
Jet Propulsion Laboratory, California Institute of Technology, Pasadena 91109.
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Grogan
D003470:Culture Media; D004352:Drug Resistance, Microbial / Q000235:genetics / Q000502:physiology; D005001:Ethionine / Q000494:pharmacology; D005472:Fluorouracil / Q000494:pharmacology; D006358:Hot Temperature; D016296:Mutagenesis; D009675:Novobiocin / Q000494:pharmacology; D010641:Phenotype; D016945:Sulfolobus acidocaldarius / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
D003470:Culture Media; D009675:Novobiocin; D005472:Fluorouracil; D005001:Ethionine