Characterization of full-length cDNAs and the gene coding for the human GM2 activator protein.
Full-length cDNAs coding for the human GM2-activator protein has been isolated and characterized, and its genomic structure studied in two overlapping clones in lambda-EMBL-4 isolated from a human brain genomic library. Two different cDNAs were found that were identical to the 5'-terminus to nt 1311 (counted from the A of the initiation codon, ATG) including the entire protein coding sequence. However, they were entirely dissimilar in the 3'-non-coding sequences. The genomic clones covered 94% of the full-length cDNA sequence. Three introns were found. The last exon spans contiguously the carboxyl terminus of the protein and the entire 3'-untranslated region of one of the two cDNAs with different 3'-ends. The origin of the 3'-portion of the other cDNA clone is not clear at this time.
Institut für Organische Chemie und Biochemie, Bonn, Germany.
H
H
Klima
A
A
Tanaka
D
D
Schnabel
T
T
Nakano
M
M
Schröder
K
K
Suzuki
K
K
Sandhoff
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005091:Exons; D049289:G(M2) Activator Protein; D005678:G(M2) Ganglioside / Q000378:metabolism; D005796:Genes; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011506:Proteins / Q000235:genetics*; D015183:Restriction Mapping
D049289:G(M2) Activator Protein; D011506:Proteins; D005678:G(M2) Ganglioside; D004247:DNA