Glucocorticoid regulation of rat liver urate oxidase.
Urate oxidase, an enzyme involved in purine catabolism, comprises the crystalline core of rat liver peroxisomes. An affinity-purified monospecific antibody was developed to study the expression of urate oxidase protein levels. Immunoreactive urate oxidase was not detectable in prenatal liver; however, it is present at low levels after birth until approximately day 15 (postnatal age); expression sharply increases just prior to day 20, after which the enzyme is maintained at adult levels. This pattern of expression was similar to that of another peroxisomal enzyme, catalase; these developmental increases reflect the increase in peroxisomal number. Administration of exogenous glucocorticoid hormone to 10-day-old rats resulted in a precocious rise (2.5-fold) in urate oxidase levels. Adrenalectomy at 10 days of age did not cause decreased levels in the fourth week of life. In adult animals, while exogenous glucocorticoid administration did not influence urate oxidase levels, adrenalectomy at 60 days of age decreased urate oxidase levels to 40 percent of control levels. Subsequent administration of exogenous glucocorticoid hormone restored urate oxidase to normal levels. Parallel studies of catalase levels indicate that this glucocorticoid-sensitive response is not generalized for all peroxisomal proteins. Our results suggest that peroxisomes proliferate during early postnatal development, but after this process is complete, the biogenesis of individual peroxisomal proteins may be independently regulated.
Department of Pediatrics, University of Texas Medical School, Houston 77030.
L S
LS
Raab
G L
GL
Decker
A J
AJ
Jonas
M A
MA
Kaetzel
J R
JR
Dedman
D000315:Adrenalectomy; D000375:Aging; D000450:Aldosterone / Q000502:physiology; D000818:Animals; D003720:Densitometry; D005938:Glucocorticoids / Q000502:physiology*; D006854:Hydrocortisone / Q000502:physiology; D007016:Hypophysectomy; D015151:Immunoblotting; D008099:Liver / Q000201:enzymology* / Q000254:growth & development / Q000648:ultrastructure; D008830:Microbodies; D016253:Microscopy, Immunoelectron; D051381:Rats; D014503:Urate Oxidase / Q000096:biosynthesis*
D005938:Glucocorticoids; D000450:Aldosterone; D014503:Urate Oxidase; D006854:Hydrocortisone