Detection of 20-hydroxyeicosatetraenoic acid in rat urine.
20-Hydroxyeicosatetraenoic acid (20-HETE), an arachidonate metabolite of the cytochrome P450 omega hydroxylase, was detected in rat urine by gas chromatography-mass spectrometric techniques. The concentration of 20-HETE in urine from 7-week-old hypertensive and normotensive rats was 2.1 and 1.3 nM, respectively. This is the first demonstration of 20-HETE urinary excretion and thus calls attention to the possibility that 20-HETE participates in the regulation of renal function via its effect on vascular tone and ion transport processes.
Department of Pharmacology, New York Medical College, Valhalla 10595.
M L
ML
Schwartzman
K
K
Omata
F M
FM
Lin
R K
RK
Bhatt
J R
JR
Falck
N G
NG
Abraham
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006893:Hydroxyeicosatetraenoic Acids / Q000652:urine*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D006893:Hydroxyeicosatetraenoic Acids; D016718:Arachidonic Acid; C055987:20-hydroxy-5,8,11,14-eicosatetraenoic acid