Oxidant-antioxidant balance: some observations from studies of ischemia-reperfusion in isolated perfused rat hearts.
Alteration in oxidant-antioxidant balance is a key feature of many common vascular diseases. Using an isolated perfused heart model, we found that (a) xanthine oxidase-derived oxygen radicals contributed to ischemia-reperfusion injury; (b) addition of antioxidants within or outside erythrocytes decreased injury following ischemia-reperfusion; (c) endotoxin pretreatment increased myocardial catalase activity and decreased injury following ischemia-reperfusion; (d) interleukin pretreatment increased myocardial glucose-6-phosphate activity and decreased ischemia-reperfusion injury, and (e) neutrophils mediated tolerance to a subsequent oxidative stress by causing a small oxidant stress that in turn increased antioxidant protection mechanisms.
Webb-Waring Lung Institute, University of Colorado Health Sciences Center, Denver.
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Repine
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D002374:Catalase / Q000378:metabolism; D004731:Endotoxins / Q000494:pharmacology; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000378:metabolism; D007375:Interleukin-1 / Q000627:therapeutic use; D008954:Models, Biological; D015428:Myocardial Reperfusion Injury / Q000139:chemically induced* / Q000188:drug therapy; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D016877:Oxidants / Q000633:toxicity*; D051381:Rats
D000975:Antioxidants; D004731:Endotoxins; D005958:Glucosephosphates; D007375:Interleukin-1; D016877:Oxidants; D019298:Glucose-6-Phosphate; D002374:Catalase