Use of nitrogen-15 determinations of purine nitrogen fraction of digesta to define nitrogen metabolism traits in the rumen.
A method for direct purine N isolation and determination was modified from the method of Zinn and Owens for rapid determination of purines. By this method, N derived from purines in digesta was measured and collected for 15N determinations. Measurements of purine N in 33 samples of ruminal contents and duodenal digesta of sheep and goats were compared with purine determinations in the same samples. The results showed that essentially all the N in the purines that could be isolated by the Zinn and Owens procedure also could be detected by N isolation and determination. Determinations of 15N in the NH3 N, NAN, and purine N fractions in a continuous culture in vitro showed significant differences in the 15N enrichment curves between diets based on either roasted or raw soybean meal.
Aharoni
Y
Y
Department of Animal Science, Faculty of Agriculture Rehovot, Israel.
Tagari
H
H
D000818:Animals; D002417:Cattle / Q000502:physiology*; D004044:Dietary Proteins / Q000378:metabolism*; D006358:Hot Temperature; D007553:Isotope Labeling; D009584:Nitrogen / Q000378:metabolism*; D009587:Nitrogen Isotopes; D011687:Purines / Q000378:metabolism*; D012044:Regression Analysis; D012417:Rumen / Q000502:physiology*; D013025:Glycine max
D004044:Dietary Proteins; D009587:Nitrogen Isotopes; D011687:Purines; D009584:Nitrogen