Glucuronidation of imipramine in rabbit and human liver microsomes: assay conditions and interaction with other tertiary amine drugs.
The present work confirms that rabbit liver microsomes afford a good in vitro model for studying the glucuronidation of drugs containing tertiary amino groups. Furthermore, the availability of the assay procedure described above will allow us to understand further the basis for the inter-species differences in the metabolism of tertiary amines, and will eventually aid in our identification of the UDPGT isozyme(s) responsible for the conjugation of these compounds in man.
Department of Biochemical Medicine, University of Dundee, Ninewells Hospital and Medical School, U.K.
M W
MW
Coughtrie
S
S
Sharp
D000588:Amines / Q000378:metabolism; D000818:Animals; D003902:Detergents; D005260:Female; D005965:Glucuronates / Q000378:metabolism*; D020723:Glucuronic Acid; D014453:Glucuronosyltransferase / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007099:Imipramine / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013329:Structure-Activity Relationship
D000588:Amines; D003902:Detergents; D005965:Glucuronates; D020723:Glucuronic Acid; D014453:Glucuronosyltransferase; D007099:Imipramine