Acetylcholine interactions with tryptophan-184 of the alpha-subunit of the nicotinic acetylcholine receptor revealed by transferred nuclear Overhauser effect.
Acetylcholine interactions with three genetically engineered fusion proteins containing peptides from the nicotinic acetylcholine receptor were studied by 1D and 2D nuclear magnetic resonance methods. The three proteins were Torpedo alpha 184-200, Torpedo alpha 186-198, and human alpha 183-204 of the acetylcholine receptor fused to the first 323 residues of the E. coli protein trpE. Nuclear Overhauser effect studies revealed interactions of bound acetylcholine with tryptophan-184 present in the Torpedo alpha 184-200, and the human alpha 183-204 sequences. These interactions are between the N(CH3)3+ and CH3 groups of acetylcholine with the aromatic protons of tryptophan. The appearance of these cross-peaks indicates a distance of less than 5 A between tryptophan and the bound ligand; however, direct contact has yet to be proven.
School of Chemistry, Tel-Aviv University, Ramat-Aviv, Israel.
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Fraenkel
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Gershoni
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Navon
D000109:Acetylcholine / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011978:Receptors, Nicotinic / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D014101:Torpedo; D014364:Tryptophan / Q000737:chemistry*
D011978:Receptors, Nicotinic; D011993:Recombinant Fusion Proteins; D014364:Tryptophan; D000109:Acetylcholine