Monoclonal antibodies to type V collagen for immunohistological examination of new tissue deposition associated with biomaterial implants.
We developed a panel of highly specific monoclonal antibodies (MAb) against dog Type V collagen. Each antibody showed differential reactivities towards Type V collagen from other species. All the antibodies were highly reactive in conventional ELISA, as well as with electroblots of collagen after polyacrylamide gel electrophoresis using non-denaturing conditions. The MAb were shown to be suitable for the immunohistological detection of Type V collagen in tissue sections, although this normally required pre-treatment of sections with 50 mM acetic acid. In particular, the antibodies were shown to be useful for examining samples of a collagen-based biomaterial, a vascular prosthesis, after explant from evaluation in an animal model. This showed that Type V collagen was most prominent in regions of new tissue formation within the neointima, close to the inner surface of the prosthesis. The broad spectrum of differential reactivities allows the antibodies to be used for a wide range of experimental models. These MAb therefore provide a novel approach for the evaluation of biomaterial performance, particularly for collagen-based implants.
Werkmeister
J A
JA
CSIRO, Division of Biomolecular Engineering, Parkville, Victoria, Australia.
Ramshaw
J A
JA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D001808:Blood Vessels / Q000737:chemistry / Q000502:physiology; D003094:Collagen / Q000032:analysis*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D019736:Prostheses and Implants; D012038:Regeneration
D000911:Antibodies, Monoclonal; D003094:Collagen