Oral Candida albicans in bone marrow transplant patients given chlorhexidine rinses: occurrence and susceptibilities to the agent.
The tongue and buccal mucosa of 26 bone marrow transplant recipients given three 0.12% chlorhexidine digluconate (CHX) oral rinses daily for 8 weeks were sampled weekly for oral Candida albicans. Putative C. albicans colony-forming units on selective bismuth sulfite glucose glycine yeast agar plates were identified with the API 20C system. The CHX minimum inhibitory concentrations (MICs) of oral C. albicans isolates obtained at all 8 sample weeks was determined with a microbroth dilution sensitivity assay. The CHX MIC range for yeast isolates selected randomly at all sample weeks was up to 2.5 to up to 20 micrograms/ml (mean MIC less than or equal to 8.5 micrograms/ml). The CHX MIC range for isolates at week 1 was less than or equal to 5 to less than or equal to 10 micrograms/ml (mean MIC less than or equal to 7.9 micrograms/ml) compared with less than or equal to 2.5 to less than or equal to 20 micrograms/ml at week 8 (mean MIC less than or equal to 8.8 micrograms/ml). Therefore the persistence of oral C. albicans in bone marrow transplant recipients using CHX rinses was due neither to low CHX susceptibilities nor to the development of resistance to the agent.
Department of Oral Health Science, College of Dentistry, Albert B. Chandler Medical Center, University of Kentucky, Lexington.
J M
JM
Thurmond
A T
AT
Brown
R E
RE
Sims
G A
GA
Ferretti
T P
TP
Raybould
T T
TT
Lillich
P J
PJ
Henslee
D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D002176:Candida albicans / Q000302:isolation & purification; D002180:Candidiasis, Oral / Q000209:etiology*; D002710:Chlorhexidine / Q000008:administration & dosage* / Q000031:analogs & derivatives; D015169:Colony Count, Microbial; D004342:Drug Hypersensitivity; D004352:Drug Resistance, Microbial; D006801:Humans; D016867:Immunocompromised Host; D007165:Immunosuppression Therapy / Q000009:adverse effects; D009061:Mouth Mucosa / Q000382:microbiology; D014059:Tongue / Q000382:microbiology
C010882:chlorhexidine gluconate; D002710:Chlorhexidine