Cochlear permeability of neomycin and gentamicin: an immunohistochemical study.
Aminoglycoside antibiotics (AGAs) target specifically the cochleo-vestibular hair cells, but with varied ototoxicity. Differences in their penetration and clearance rates into the membranous labyrinth may play a role. This in turn may be related to a difference in the number of amine groups, the cationic nature, as well as the molecular weight and size of the AGA molecule. Immunohistochemical labeling techniques were used to study the pathways of gentamicin and neomycin from the perilymph into cochlear tissues and target cells. The more cochleotoxic AGA, neomycin, penetrated into cochlear tissues faster than the less cochleotoxic AGA, gentamicin.
Duvall
A J
AJ
Department of Otolaryngology, University of Minnesota Research East, Minneapolis 55414.
Robinson
K S
KS
Feist
S J
SJ
D000818:Animals; D001489:Basilar Membrane / Q000378:metabolism; D002682:Chinchilla; D003051:Cochlea / Q000033:anatomy & histology / Q000378:metabolism*; D003053:Cochlear Duct / Q000378:metabolism; D005839:Gentamicins / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006199:Hair Cells, Auditory, Inner / Q000378:metabolism; D007150:Immunohistochemistry; D009355:Neomycin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D009925:Organ of Corti / Q000378:metabolism; D010498:Perilymph / Q000378:metabolism; D010539:Permeability; D012533:Scala Tympani / Q000378:metabolism; D013316:Stria Vascularis / Q000378:metabolism; D013997:Time Factors
D005839:Gentamicins; D009355:Neomycin