Microwave oven for improved tissue fixation and decalcification.
The present study reports on our experience on microwave (MW) fixation and decalcification. A common kitchen microwave oven was used. Autoptical material and biopsies from different organs were immersed in 10% formalin and MW irradiated varying time and power. Bone biopsies were immersed in 5% formic acid and MW irradiated. The tissues were then routinely embedded in paraffin. The results, checked on routinely stained sections, indicate that MW irradiation speeds up both fixation and decalcification. Optimal treatment conditions are indicated.
Department of Biomedical Sciences and Human Oncology, University of Turin, Torino, Italy.
F
F
Roncaroli
B
B
Mussa
G
G
Bussolati
D003648:Decalcification Technique / Q000295:instrumentation*; D006801:Humans; D008872:Microwaves; D016707:Tissue Fixation / Q000295:instrumentation*