Studies on eye irritation caused by chemicals in rabbits--II. An in vitro testing method using rat red blood cells for the prediction of eye irritation potential of chemicals.
Rat red blood cells were used as an in vitro method to evaluate the eye irritation potential of chemicals in rabbits. The results using 116 chemicals of various categories including medicines, pesticides, detergents and solvents were analyzed for the prediction of possibility of eye irritation potentials. Eye irritation of chemicals was examined according to Draize method and chemicals were classified into three categories, (1) non or mild irritants, (2) moderate or severe irritants and (3) strong or corrosive irritants, based on the recovery of damages. The in vitro method consisted of two methods detecting the effects of chemicals mainly on protein and lipid in the membrane, which were evaluated by the induction of methemoglobin and hemolysis, respectively. Non- or mild irritants induced neither methemoglobin formation nor hemolysis. Most of moderate or severe irritants induced hemolysis, however, the potentials were low. Strong or corrosive irritants had high potentials for the induction of methemoglobin. The multivariate estimation by the above two in vitro data sets were 77.6% predictive of the in vivo classification.
Sugai
S
S
Life Science Research Institute, Kumiai Chemical Co. Ltd., Shizuoka, Japan.
Murata
K
K
Kitagaki
T
T
Tomita
I
I
D000818:Animals; D004912:Erythrocytes / Q000187:drug effects; D005123:Eye / Q000187:drug effects*; D005260:Female; D006461:Hemolysis / Q000187:drug effects*; D066298:In Vitro Techniques; D007509:Irritants / Q000009:adverse effects*; D008297:Male; D008706:Methemoglobin / Q000378:metabolism*; D008722:Methods; D011817:Rabbits; D051381:Rats; D011916:Rats, Inbred F344
D007509:Irritants; D008706:Methemoglobin