Site-directed mutagenesis of Escherichia coli succinyl-CoA synthetase. Histidine 142 alpha is a facilitative catalytic residue.
There are 11 histidine residues in Escherichia coli succinyl-CoA synthetase. His-246 alpha is well established as the phosphorylation site of the enzyme. Replacement of this histidine by asparagine (Mann, C. J., Mitchell, T., and Nishimura, J. S. (1991) Biochemistry 30, 1497-1503) or by aspartic acid (Majumdar, R., Guest, J. R., and Bridger, W. A. (1991) Biochim. Biophys. Acta 1076, 86-90) through site-directed mutagenesis resulted in complete loss of enzyme activity. Chemical modification experiments suggested a second histidine at the active site (Collier, G. E., and Nishimura, J. S. (1979) J. Biol. Chem. 254, 10925-10930). In the present study, we have changed His-142 alpha to an asparagine residue using the technique of site-directed mutagenesis and have purified the mutant enzyme to homogeneity. The resulting mutant enzyme is practically devoid of enzyme activity but can be thiophosphorylated with adenosine 5'-O-(thiotriphosphate) and dethiophosphorylated with ADP at rates that are significantly faster than those with wild type enzyme. The observation that phosphorylated mutant enzyme can be dephosphorylated with succinate and with succinate plus desulfo-CoA at rates comparable with those with wild type enzyme suggests that mutant enzyme can bind succinate and CoA. Dethiophosphorylation of the enzyme in the presence of CoA plus succinate proceeds much faster with wild type than with mutant. While there was no significant change in KCoA or Ksuccinate, the turnover number for dethiophosphorylation of the mutant was 10-fold lower. These data are consistent with location of His-142 alpha at the active site and a facilitative role for this residue in catalysis.
Luo
G X
GX
Department of Biochemistry, University of Texas Health Science Center, San Antonio 78284.
Nishimura
J S
JS
D000214:Acyl Coenzyme A / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002384:Catalysis; D004252:DNA Mutational Analysis; D004926:Escherichia coli / Q000201:enzymology*; D006639:Histidine / Q000502:physiology; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D010710:Phosphates / Q000378:metabolism; D010766:Phosphorylation; D013329:Structure-Activity Relationship; D013389:Succinate-CoA Ligases / Q000235:genetics* / Q000378:metabolism; D013386:Succinates / Q000378:metabolism
D000214:Acyl Coenzyme A; D009841:Oligonucleotides; D010710:Phosphates; D013386:Succinates; D006639:Histidine; C012046:succinyl-coenzyme A; D013389:Succinate-CoA Ligases; C035638:thiophosphoric acid