The role of NADPH in the regulation of glucose-6-phosphate and 6-phosphogluconate dehydrogenases in rat adipose tissue.
Previous studies examining the regulation of the synthesis of G6PDH and 6PGDH in rat liver and adipose tissue have focused on the induction of these enzymes by different diets and some hormones. In rat liver these enzymatic activities seem to be regulated by a mechanism involving changes in the NADPH requirements. In this paper we have studied the effect of changes in the flux through different NADPH-consuming pathways on G6PDH and 6PGDH levels in adipose tissue and on the NADPH/NADP ratio. The results show that: I) an increase in the consumption of NADPH, caused by the activation of either fatty acid synthesis or detoxification systems which consume NADPH, is paralleled by an increase in the levels of these enzymes; II) when the increase in consumption of NADPH is prevented, the G6PDH and 6PGDH levels do not change.
Departamento de Bioquimica, Facultad de Farmacia, Universidad de Sevilla, Spain.
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Ayala
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Fabregat
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Machado
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology; D001629:Bezafibrate / Q000494:pharmacology; D050260:Carbohydrate Metabolism; D005260:Female; D005508:Food Deprivation / Q000502:physiology; D019298:Glucose-6-Phosphate; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D005958:Glucosephosphates / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D009249:NADP / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009582:Nitrofurantoin / Q000494:pharmacology; D010545:Peroxides / Q000494:pharmacology; D010734:Phosphogluconate Dehydrogenase / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D020122:tert-Butylhydroperoxide
D001555:Benzene Derivatives; D005958:Glucosephosphates; D007328:Insulin; D010545:Peroxides; D009249:NADP; D019298:Glucose-6-Phosphate; D009582:Nitrofurantoin; D020122:tert-Butylhydroperoxide; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; C007164:cumene hydroperoxide; D001629:Bezafibrate