Postembedding immunogold histochemistry for the localization of laminin and its E4 and P1 fragments in mouse kidney embedded in LR-White and LR-Gold.
A method is presented which permits the ultrastructural localization of laminin and its E4 and P1 subunits in the renal cortex of the mouse embedded in LR-White or LR-Gold. It was performed with postembedding immunogold histochemistry using polyclonal antibodies against either the entire laminin molecular or the E4 fragment or with a monoclonal antibody against the P1 fragment. Localization of laminin was achieved in LR-White and in LR-Gold embedded kidney. Using polyclonal antibodies against the entire laminin molecule, laminin could be localized with direct as well as with indirect immunogold histochemistry with a gold labelled IgG as secondary antibody. In contrast, immunostaining for the E4 or the P1 fragments was possible only with antibodies directly labelled with gold.
Zentrum Anatomie, Abteilung Histologie, Göttingen, Germany.
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Herken
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Miosge
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007150:Immunohistochemistry; D007672:Kidney Cortex / Q000737:chemistry* / Q000648:ultrastructure; D007797:Laminin / Q000032:analysis* / Q000737:chemistry; D051379:Mice; D010446:Peptide Fragments / Q000032:analysis*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D007797:Laminin; D010446:Peptide Fragments