Late phase increase of thrombin-like proteinase, protein, leukocytes in bronchoalveolar lavage (BAL) fluid and chemiluminescence of BAL after ovalbumin aerosol inhalation by actively immunized rats.
A thrombin-like proteinase (THROLP) was detected colorimetrically as the main proteolytic activity (PROA) in bronchoalveolar lavage fluid (BALF) 4 and 24 h after ovalbumin aerosol (OVA) challenge of actively immunized rats. Other proinflammatory parameters increased also significantly in BALF, like chemiluminescence of leukocytes (1 h), protein and cell number (24 h after challenge). In parallel, increased bronchoconstrictions against 5-HT aerosols are detected 24 h post OVA challenge. THROLP is an indicator for plasma leakage, activation of the clotting reaction and the protracted inflammation in the airways, which induced bronchial hyperreactivity.
Medical Research, Boehringer Mannheim GmbH, FRG.
M
M
Linssen
C
C
Lemmermann
L
L
Lipponer
O H
OH
Wilhelms
D000280:Administration, Inhalation; D000336:Aerosols; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000166:cytology; D010450:Endopeptidases / Q000032:analysis*; D007114:Immunization; D008163:Luminescent Measurements; D010047:Ovalbumin / Q000276:immunology*; D011506:Proteins / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000494:pharmacology; D013917:Thrombin / Q000032:analysis*
D000336:Aerosols; D011506:Proteins; D012701:Serotonin; D010047:Ovalbumin; D010450:Endopeptidases; D013917:Thrombin