Detection of HBV-DNA using a digoxigenin-labelled probe. A rapid technique without loss of sensitivity.
The sensitivity of four different non-radiolabelled HBV-DNA probes (digoxigenin-, biotin-, acetylaminoflorene-, chemiprobe-labelled) in detecting HBV-DNA were compared with a 32P-labelled HBV-DNA probe using a spot hybridisation assay. Of the non-radiolabelled probes, the probe using digoxigenin labelling was the most sensitive with reliable and reproducible detection to between 1-3 pg HBV-DNA. The use of digoxigenin-labelled HBV-DNA hybridisation assay deserves wider application for the detection of HBV-DNA because of a number of advantages including ease, speed, safety and cost.
Naoumov
N V
NV
Institute of Liver Studies, King's College Hospital, King's College School of Medicine and Dentistry, London, United Kingdom.
Lau
J Y
JY
Daniels
H M
HM
Alexander
G J
GJ
Williams
R
R
D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D004076:Digoxigenin; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D012680:Sensitivity and Specificity; D013997:Time Factors
D015342:DNA Probes; D004279:DNA, Viral; D010761:Phosphorus Radioisotopes; D004076:Digoxigenin