A novel assay for the in vivo study of Schwann cells.
An in vivo assay was developed for long-term analyses of Schwann cells at the single cell level. Schwann cells were isolated from sciatic nerves and labeled with the fluorescent gold label Fluoro-Gold. Cells were then transplanted into severed sciatic nerves of young adult rats. Gold-labeled Schwann cells, identified by double-labeling with S100 antibodies, were observed up to 10 mm away from the transplant site and after 90 and 120 days of survival in vivo.
Daniloff
J K
JK
Louisiana State University School of Veterinary Medicine, Department of Veterinary Anatomy and Fine Structure, Baton Rouge 70803.
D000818:Animals; D000906:Antibodies; D002465:Cell Movement; D002469:Cell Separation; D002470:Cell Survival; D003584:Cytological Techniques; D005456:Fluorescent Dyes; D009418:S100 Proteins / Q000276:immunology; D012583:Schwann Cells / Q000502:physiology* / Q000637:transplantation; D012584:Sciatic Nerve / Q000166:cytology / Q000502:physiology; D013266:Stilbamidines; D013997:Time Factors
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D000906:Antibodies; D005456:Fluorescent Dyes; D009418:S100 Proteins; D013266:Stilbamidines