[Immunohistochemical observation on the proliferative activity of rat liver in early hepatocarcinogenesis].
Anti-BrdU immunoperoxidase staining was used for cytogenetic investigation of altered foci and nodules in rat liver induced by a procedure including intraperitoneal injection of diethylnitrosamine (DEM), feeding of 2-acetoaminoflurene (2-AAF) and 2/3 hepatectomy. Variation in number and size of the altered foci as well as the number of BrdU-positive hepatocytes inside and outside the foci were clearly observed in the early stage of hepatocarcinogenesis. A predominant distribution of BrdU-positive cells was obtained in the peripheral zone of the altered nodules favorable in explaining the phenomenon of monoclonal origin of the altered hepatocytes inside the nodules. This study also indicated that BrdU-immunoperoxidase staining is a reliable and convenient method in studying the dynamic change of DNA-replication in the process of active hepatocellular proliferation.
Department of Pathology, Fujian Medical College, Fuzhou.
Y J
YJ
Xie
D000818:Animals; D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine / Q000276:immunology; D004052:Diethylnitrosamine; D005260:Female; D006965:Hyperplasia; D007150:Immunohistochemistry; D008099:Liver / Q000473:pathology*; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D008297:Male; D011230:Precancerous Conditions / Q000139:chemically induced / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D004052:Diethylnitrosamine; D001973:Bromodeoxyuridine