Regulation of ribosome biogenesis in differentiated rat myotubes.
The rate of ribosome biogenesis is closely coupled with cell proliferation, representing a unique model system for studying gene regulation. Terminal differentiation of rat L6 myoblasts, an example of a rapidly proliferating population of cells being converted into a non-dividing syncytial population, results in an 80% decline in the rate of ribosome accumulation. Ribosome production during myogenesis is regulated by a down-shift in the rate of rRNA accumulation, controlled at the level of transcription by specific trans-acting factors. The synthesis of both ribosomal proteins and 5S rRNA remains unchanged in myotubes, however, resulting in an over-production of these precursors. The excess molecules are rapidly degraded, preventing the accumulation of a static pool of ribosome components.
Department of Molecular Biology and Genetics, University of Guelph, Ontario, Canada.
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P
Zahradka
D E
DE
Larson
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BH
Sells
D000818:Animals; D002454:Cell Differentiation; D009132:Muscles / Q000166:cytology / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000096:biosynthesis; D051381:Rats; D012269:Ribosomal Proteins / Q000096:biosynthesis; D012270:Ribosomes / Q000378:metabolism*
D012335:RNA, Ribosomal; D012269:Ribosomal Proteins