The effects of denatured sodium hyaluronate on the corneal endothelium in cats.
In an in vivo cat model, wide-field specular microscopy and scanning electron microscopy were used to observe whether heat-denatured sodium hyaluronate causes cell damage to the corneal endothelium. The endothelial cell toxicities of various drugs, including 0.01% benzalkonium chloride, 0.2% chlorhexidine digluconate, and 0.5% lidocaine, mixed with intact or untreated sodium hyaluronate were also investigated. Neither heat-denatured nor intact or untreated sodium hyaluronate alone had any adverse effect on the corneal endothelium in cats, whereas 0.01% benzalkonium chloride and 0.2% chlorhexidine digluconate, mixed with sodium hyaluronate, caused substantial endothelial morphologic changes, which resulted in corneal edema. By comparison, 0.5% lidocaine with sodium hyaluronate was found to have minimal effect on the corneal endothelium in cats. These findings indicate that some chemical contaminant with sodium hyaluronate, not heat-denatured sodium hyaluronate, induces an immediate onset of pseudophakic bullous keratopathy.
Department of Ophthalmology, Osaka University Medical School, Japan.
N
N
Ohguro
M
M
Matsuda
S
S
Kinoshita
D000818:Animals; D001548:Benzalkonium Compounds / Q000633:toxicity; D002415:Cats; D002452:Cell Count / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002710:Chlorhexidine / Q000633:toxicity; D015715:Corneal Edema / Q000139:chemically induced; D004728:Endothelium, Corneal / Q000187:drug effects* / Q000648:ultrastructure; D006820:Hyaluronic Acid / Q000633:toxicity*; D008012:Lidocaine / Q000633:toxicity; D008855:Microscopy, Electron, Scanning; D011489:Protein Denaturation
D001548:Benzalkonium Compounds; D006820:Hyaluronic Acid; D008012:Lidocaine; D002710:Chlorhexidine