Effect of different amino acidic pretreatments that protect the kidney against papillary necrosis induced by bromoethylamine on differential distribution of renal nonprotein sulfhydryls.
Content of nonprotein sulfhydryls (NPSH) was found to be higher in rat renal cortex than in external medulla and papilla. Administration of bromoethylamine (BEA), at a dose that produces extensive papillary necrosis and minor effects in the other renal segments, induced a significant reduction in NPSH levels of renal cortex and external medulla, with no changes in the papilla. Treatment with N-acetyl-L-cysteine (NAC) elicited an increase in papillary NPSH and a decrease in the cortex, with opposite changes being observed with an amino acid mixture of glutamine, glycine, and cystine (AM). Similar results were found in animals pretreated with NAC or AM prior to BEA intoxication. These pretreatments protect the cortex, external medulla, and papilla from the necrosis induced by BEA. It is suggested that protection of BEA-induced renal necrosis by NAC or AM pretreatments might be due to different mechanisms, with NPSH playing direct or indirect roles, respectively.
Departamento de Medicina Experimental, Facultad de Medicina, Universidad de Chile, Santiago.
L E
LE
Thielemann
G
G
Rosenblut
M C
MC
Cerda
E W
EW
Oberhauser
M A
MA
De Geyter
L A
LA
Videla
D000111:Acetylcysteine / Q000494:pharmacology; D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D003553:Cystine / Q000378:metabolism; D005021:Ethylamines; D005973:Glutamine / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism; D007679:Kidney Medulla / Q000187:drug effects / Q000378:metabolism; D007681:Kidney Papillary Necrosis / Q000139:chemically induced / Q000517:prevention & control*; D051381:Rats; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D000596:Amino Acids; D005021:Ethylamines; D013438:Sulfhydryl Compounds; D005973:Glutamine; C004504:2-bromoethylamine; D003553:Cystine; D005998:Glycine; D000111:Acetylcysteine