Probing nucleosome core secondary structure before and after alpha-chymotrypsin treatment by Raman spectroscopy and thermal denaturation.
Nucleosome cores were digested with alpha-chymotrypsin until histone H3 was degraded to a partial histone, CP1. As we reported previously, cleavage occurred at leucine 20 to H3 and resulted in an increase in circular dichroism between 265 to 285 nm. Some modest core unfolding was also observed as determined by a small decrease in the sedimentation coefficient. Studies reported here deal with the analysis of core secondary structure and subsequent perturbation caused by treatment with alpha-chymotrypsin. Raman spectroscopy indicated that chymotryptic treatment promoted a change in the conformational environment of a population of core histone tyrosines. In addition, a shift from B-form to an intermediate B- or A-form was observed for core DNA. High-resolution thermal denaturation was used to determine alterations in the stabilization of core DNA related to perturbation of the core histones. Brief chymotryptic treatment indicated changes in both pre-melt and irreversible transitions.
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Department of Tumor Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
D000818:Animals; D002645:Chickens; D002918:Chymotrypsin / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000378:metabolism; D015394:Molecular Structure; D009707:Nucleosomes / Q000737:chemistry*; D011487:Protein Conformation; D011489:Protein Denaturation; D013059:Spectrum Analysis, Raman; D013696:Temperature
D006657:Histones; D009707:Nucleosomes; D004247:DNA; D002918:Chymotrypsin