[Oxidative modification of cytochrome P-450 during its function. I. Comparative study of the inactivation of cytochrome P-450 LM2 in various systems].
The changes in the content of purified isolated cytochrome P-450 LM2 under the action of hydrogen peroxide and during its operation in a soluble reconstituted system were studied. It was found that cytochrome P-450 LM2 inactivation by hydrogen peroxide is accompanied by a decrease in the hemoprotein activity, loss of heme, oxidation of SH-groups and changes in the oligomeric state of the enzyme. There were some differences in the mechanisms of cytochrome P-450 LM2 inactivation under the action of H2O2 and during catalysis.
Tret'iakova
L Z
LZ
Adrianov
N V
NV
Dzhuzenova
Ch S
ChS
Dovgiĭ
A I
AI
Archakov
A I
AI
D000818:Animals; D002384:Catalysis; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003902:Detergents; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000378:metabolism
D065607:Cytochrome P-450 Enzyme Inhibitors; D003902:Detergents; D013438:Sulfhydryl Compounds; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D006861:Hydrogen Peroxide
Okislitel'naia modifikatsiia tsitokhroma P-450 pri ego rabote. I. Sravnitel'noe issledovanie inaktivatsii tsitokhroma P-450 LM2 v razlichnykh sistemakh.