A new method for faster blood clearance in radioimmuno-guided surgery.
A major inconvenience of intraoperative tumor detection with radiolabeled monoclonal antibody (MoAb) by means of a probe is the long interval needed between the injection of MoAb and surgery (at least 20 days). We describe a new method which entails injection of 125I-labeled biotinylated monoclonal antibodies and cold avidin in order to obtain a rapid blood clearance of the radiotracer. The first clinical application of this protocol has been carried out in a colon cancer patient operated on six days after the administration of 125I-labeled biotinylated MoAb and cold avidin, with an optimal tumor-to-normal tissue ratio and with 90% of the circulating radioactivity cleared in two days.
ITBA-CNR, University of Milan Scientific Institute H San Raffaele, Italy.
G
G
Paganelli
M
M
Stella
P
P
De Nardi
P
P
Magnani
F
F
Zito
A G
AG
Siccardi
V
V
Di Carlo
F
F
Fazio
D000008:Abdominal Neoplasms / Q000000981:diagnostic imaging / Q000556:secondary / Q000601:surgery*; D000368:Aged; D001360:Avidin; D003110:Colonic Neoplasms / Q000473:pathology; D006801:Humans; D007430:Intraoperative Care / Q000379:methods; D008297:Male; D016719:Radioimmunodetection; D013997:Time Factors
D001360:Avidin