A simple method for ultrastructural enzyme cytochemistry on small volumes and numbers of isolated cells.
A simple technique is described for ultrastructural enzyme cytochemistry using small volumes and numbers of single cells. Small aliquots (1-5 microliters) of single cells suspended in a glutaraldehyde fixative are incubated on poly-L-lysine-coated glass coverslips. Cells in contact with the coated coverslip become immobilized to that support. All changes of solutions are accomplished without repeated centrifugation.
Robinson
J M
JM
Department of Cell Biology, Neurobiology and Anatomy, Ohio State University, Columbus 43210.
D015720:5'-Nucleotidase / Q000032:analysis*; D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D002460:Cell Line / Q000201:enzymology; D016591:Histocytological Preparation Techniques; D006801:Humans; D051379:Mice; D009000:Monocytes / Q000201:enzymology* / Q000648:ultrastructure; D011107:Polylysine
D011107:Polylysine; D000135:Acid Phosphatase; D015720:5'-Nucleotidase