Membranolytic and nucleolytic activities of cytolytic T lymphocyte clones.
In an attempt to see if the nucleolytic and membranolytic activities of cytolytic T lymphocytes (CTL) were totally independent and could be expressed independently, we cloned CTL and determined their membranolytic and nucleolytic activities. If the two lytic mechanisms were completely independent and could be independently expressed by individual CTL, we anticipated that we would find CTL clones exhibiting only one or the other activity. Initial examination of membranolytic and nucleolytic activities in 99 newly established CTL clones revealed a poor correlation (r = 0.4) between the two activities. In addition, some clones expressed membenolytic activity without nucleolytic activity, and others, nucleolytic activity without membenolytic activity. The results suggest that CTL have 2 or more separate and independent mechanisms that lead either to the membranolytic or to the nucleolytic lesions in target cells.
Department of Pathology, University of Connecticut School of Medicine, Farmington 06030.
J M
JM
Cosgrove
T K
TK
Howcroft
S M
SM
Tatum
R R
RR
Lindquist
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000648:ultrastructure*; D003602:Cytotoxicity, Immunologic; D005260:Female; D012515:Mast-Cell Sarcoma / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*