Transformation of Aspergillus terreus with the hygromycin B resistance marker from Escherichia coli.
Aspergillus terreus was transformed to hygromycin B resistance using a bacterial resistance gene under the control of Aspergillus nidulans regulatory sequences. Southern hybridization of transformants indicated that in most of the cases the vector DNA was integrated into the recipient chromosome in the form of tandem arrays. Transformants were mitotically stable in both selective and non-selective medium and retained their capacity to produce xylanase or glucoamylase activities.
Unidad de Bioingeniería de los Alimentos, Instituto de Agroquímica y Tecnología de los Alimentos (C.S.I.C.), Valencia, Spain.
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D001230:Aspergillus / Q000187:drug effects / Q000235:genetics*; D001233:Aspergillus nidulans / Q000187:drug effects / Q000235:genetics; D015139:Blotting, Southern; D002934:Cinnamates; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D014170:Transformation, Genetic; D043325:Xylan Endo-1,3-beta-Xylosidase
D002934:Cinnamates; D005819:Genetic Markers; D006921:Hygromycin B; C026273:hygromycin A; D006026:Glycoside Hydrolases; D005087:Glucan 1,4-alpha-Glucosidase; D043325:Xylan Endo-1,3-beta-Xylosidase