Temporal control for translation of influenza virus mRNAs.
cDNAs for genome RNAs of influenza virus A/PR/8/34 were cloned and portions containing the ATG initiation codon for translation were inserted into the 5' leader sequence of the chloramphenicol acetyltransferase (CAT) gene in a pSV2cat vector. In cells that were transfected with a plasmid containing a cDNA segment for the early gene and then super-infected with influenza virus, the maximal CAT activity was obtained at the early stage of infection. In contrast, a plasmid containing a cDNA segment for the late gene directed the highest activity at the late stage of infection. These observations together with the previous observations [K. Yamanaka et al. (1988) Virus Genes 2: 19-30] indicate that the translational efficiency of influenza viral mRNA is subjected to temporal control following viral infection.
Department of Molecular Genetics, National Institute of Genetics, Shizuoka, Japan.
K
K
Yamanaka
K
K
Nagata
A
A
Ishihama
D000595:Amino Acid Sequence; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D003062:Codon; D004279:DNA, Viral / Q000032:analysis; D003854:Deoxyribonucleotides; D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells; D006801:Humans; D009980:Influenza A virus / Q000235:genetics*; D008969:Molecular Sequence Data; D010442:Peptide Chain Initiation, Translational; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism; D014162:Transfection
D003062:Codon; D004279:DNA, Viral; D003854:Deoxyribonucleotides; D012333:RNA, Messenger; D012367:RNA, Viral; D015500:Chloramphenicol O-Acetyltransferase