Murine retroviral vectors expressing the tax1 gene of human T-cell leukemia virus type 1.
Murine retroviral vectors which express the tax1 gene of human T-cell leukemia virus type 1 (HTLV-1) have been constructed. A significant increase in virus titer was achieved by inserting a portion of the gag region of Moloney murine leukemia virus. Using these vectors, tax1 was stably introduced into primary human T-cells derived from peripheral blood lymphocytes. Expression of the functional tax1 in infected cells was confirmed by trans-activation of HTLV-1 long terminal repeat-directed transcription. These vectors provide a useful means of investigating the function of tax1 in natural target cells for HTLV-1.
Virology Division, National Cancer Center Research Institute, Tokyo, Japan.
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Akagi
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Nyunoya
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Shimotohno
D000818:Animals; D015139:Blotting, Southern; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D015342:DNA Probes / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D015750:Genes, gag / Q000235:genetics; D016355:Genes, pX / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D013601:T-Lymphocytes / Q000382:microbiology; D015533:Transcriptional Activation / Q000235:genetics; D014170:Transformation, Genetic / Q000235:genetics
D015342:DNA Probes; D004274:DNA, Recombinant; D015500:Chloramphenicol O-Acetyltransferase