Development of a differential volume reactor system for soil biodegradation studies.
A bench scale experimental system was developed for the analysis of polycyclic aromatic hydrocarbon (PAH) degradation by mixed microbial cultures in PAH contaminated Manufactured Gas Plant (MGP) soils and on sand. The reactor system was chosen in order to provide a fundamental protocol capable for evaluating the performance of specific mixed microbial cultures on specific soil systems by elucidating the important system variables and their interactions. The reactor design and peripherals are described. A plug flow differential volume reactor (DVR) was used in order to remove performance effects related to reactor type, as opposed to system structure. This reactor system could be well represented mathematically. Methods were developed for on-line quantitative determination of PAH liquid phase concentrations. The mathematical models and experimental data are presented for the biodegradation of naphthalene on artificial and MGP soils.
Center for Environmental Biotechnology, University of Tennessee-Knoxville 37996-2200.
O F
OF
Webb
T J
TJ
Phelps
P R
PR
Bienkowski
P M
PM
DiGrazia
G D
GD
Reed
B
B
Applegate
D C
DC
White
G S
GS
Sayler
D001419:Bacteria / Q000201:enzymology*; D001711:Biotransformation; D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D049308:Dioxygenases; D007202:Indicators and Reagents; D007506:Iron-Sulfur Proteins / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010100:Oxygen / Q000032:analysis; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism; D011083:Polycyclic Compounds / Q000032:analysis*; D016133:Polymerase Chain Reaction; D012987:Soil / Q000032:analysis*; D012989:Soil Pollutants / Q000032:analysis*
D007202:Indicators and Reagents; D007506:Iron-Sulfur Proteins; D009097:Multienzyme Complexes; D011083:Polycyclic Compounds; D012987:Soil; D012989:Soil Pollutants; D010105:Oxygenases; D049308:Dioxygenases; C038703:naphthalene dioxygenase; D010100:Oxygen