Membrane-incorporated immunoglobulin receptors increase the antigen-presenting ability of B cells.
Monoclonal antibodies specific for ovalbumin were conjugated to palmitate and inserted into the membrane of normal spleen B cells. Their presence in the membrane, as well as their ability to bind ovalbumin, was established by immunofluorescence. The so called anti-ovalbumin-'decorated' B cells were tested for their ability to act as antigen-presenting cells for ovalbumin-specific I-Ad-restricted T-cell hybridomas. It was found that the antibody-decorated B cells presented antigen more efficiently than non-decorated B cells.
Gontijo
C M
CM
Department of Biochemistry and Immunology, Federal University of Minas Gerais, Brazil.
Möller
G
G
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D006825:Hybridomas / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000502:physiology*; D011971:Receptors, Immunologic / Q000502:physiology*
D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell; D011971:Receptors, Immunologic; D010047:Ovalbumin