Isolation of a mRNA that encodes a putative intestinal alkaline phosphatase regulated by 1,25-dihydroxyvitamin D-3.
Using differential hybridization techniques to screen a rat intestinal cDNA library we isolated a cDNA whose predicted amino acid sequence exhibits a high degree of homology to the alkaline phosphatases. The predicted cDNA sequence has 79% identity at the amino acid level to the rat intestinal alkaline phosphatase, and shows approx. 70% homology to other human and rat alkaline phosphatases. The corresponding mRNA is markedly increased by 6 h after a single dose of 1,25-dihydroxyvitamin D-3. The mRNA is also increased by 24-homologated analogs of 1,25-dihydroxyvitamin D-3 that do not increase calcium transport.
Strom
M
M
Department of Biochemistry, College of Agricultural and Life Sciences, Madison, WI.
Krisinger
J
J
DeLuca
H F
HF
D000469:Alkaline Phosphatase / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002117:Calcitriol / Q000494:pharmacology*; D004247:DNA; D007422:Intestines / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D016415:Sequence Alignment
D012333:RNA, Messenger; D004247:DNA; D000469:Alkaline Phosphatase; D002117:Calcitriol