A magnetizable solid phase for enzyme extraction.
A method for the convenient and reliable preparation of magnetizable agarose beads containing iron particles is described. The beads were treated with the triazine dye, Reactive Red 120, and the matrix was examined for the ability to extract proteins from crude preparations using lactate dehydrogenase from porcine muscle as a model. The recovery and specific activity values of enzyme obtained using this matrix and magnetic field separation were significantly greater than those for enzyme purified by centrifugation and conventional dye ligand chromatography.
Ennis
M P
MP
Division of Biochemistry, Queen's University of Belfast, Northern Ireland, UK.
Wisdom
G B
GB
D000327:Adsorption; D000818:Animals; D001709:Biotechnology; D004396:Coloring Agents; D004798:Enzymes / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D007501:Iron; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification; D008280:Magnetics; D009132:Muscles / Q000201:enzymology; D012685:Sepharose; D013552:Swine; D014227:Triazines
D004396:Coloring Agents; D004798:Enzymes; D014227:Triazines; C042516:reactive red 120 dye; D012685:Sepharose; D007501:Iron; D007770:L-Lactate Dehydrogenase