Renal tubular epithelial antigen-containing immune complexes stimulate interleukin-1 production by cultured rat glomerular mesangial cells.
The ability of immune complexes (IC) derived from rat renal tubular epithelial (RTE) antigen to trigger the production of interleukin-1 (IL-1) was investigated. When cultured rat mesangial cells (MC) were activated by the IC, substantial amounts of IL-1 could be detected in the culture supernatants as measured by the mouse thymocyte assay. To avoid the effect of prostaglandins on the IL-1 assay, we cultured the MC with addition of indomethacin and assayed IL-1 activity in the culture supernatants. This cyclooxygenase inhibitor augmented IC-induced IL-1 production. These biological activities were neutralized by a specific antibody to IL-1. These findings suggest that IC may participate in local inflammatory responses in the kidney partly due to their capacity to induce the synthesis of MC IL-1.
Matsumoto
K
K
Second Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan.
Hatano
M
M
D000818:Animals; D000936:Antigen-Antibody Complex; D000941:Antigens; D002478:Cells, Cultured; D004848:Epithelium / Q000276:immunology; D005920:Glomerular Mesangium / Q000276:immunology*; D007074:Immunoglobulin G; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis*; D007684:Kidney Tubules / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009500:Neutralization Tests; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology
D000936:Antigen-Antibody Complex; D000941:Antigens; D007074:Immunoglobulin G; D007375:Interleukin-1; C035812:renal tubular antigen