Human carboxypeptidase A identifies a BglII RFLP and maps to 7q31-qter.
A genomic clone for human carboxypeptidase has been isolated with a probe for rat CPA1 cDNA. A 1.7-kb HindIII/EcoRI fragment from the 3' flanking region of human carboxypeptidase detects a DNA polymorphism with BglIII. Multipoint linkage analysis with an established map of chromosome 7 markers shows that the most likely location of carboxypeptidase is at 7q31-qter, between D7S87 and D7S93. All other placements can be excluded with odds greater than 100:1. These and other markers confirm that carboxypeptidase lies distal to the locus for cystic fibrosis, at a distance of approximately 12 centimorgans. The regions containing identity to the rat gene were sequenced and shown to be 82% identical to exons 9 and 10 of the rat gene. The presence of a codon for isoleucine at the residues corresponding to codon 255 of rat CPA1 cDNA strongly suggests that the A form of human carboxypeptidase has been isolated.
Stewart
E A
EA
Department of Genetics, Stanford University Medical Center, CA 94305.
Craik
C S
CS
Hake
L
L
Bowcock
A M
AM
D001426:Bacterial Proteins; D001483:Base Sequence; D002268:Carboxypeptidases / Q000235:genetics*; D043422:Carboxypeptidases A; D002897:Chromosomes, Human, Pair 7; D004247:DNA / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D005819:Genetic Markers; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D001426:Bacterial Proteins; D005819:Genetic Markers; D004247:DNA; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific; D002268:Carboxypeptidases; D043422:Carboxypeptidases A