The solution conformation of the Le chi group.
The solution conformation of the non-reducing terminal Gal beta 1----4 (Fuc alpha 1----3)GlcNAc (Lewis X or Le chi) group in the oligosaccharide Lacto-N-fucopentaose (LNFP) III has been determined by high resolution 1H NMR spectroscopy and semi-empirical quantum mechanical calculations. The two methods give the same single conformer for the Le chi group showing close packing of the Gal and Fuc rings. The metal binding properties and homotypic oligomer formation of LNFP III have also been investigated by NMR spectroscopy. No evidence for metal binding or high-affinity homotypic oligomer formation has been found.
Wormald
M R
MR
Department of Biochemistry, University of Oxford, U.K.
Edge
C J
CJ
Dwek
R A
RA
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006801:Humans; D007983:Lewis Blood Group Antigens; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D012996:Solutions; D013816:Thermodynamics
D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides; D012996:Solutions