Cloning of cDNAs for human phosphoribosylpyrophosphate synthetases 1 and 2 and X chromosome localization of PRPS1 and PRPS2 genes.
Cloned cDNAs representing the entire, homologous (80%) translated sequences of human phosphoribosylpyrophosphate synthetase (PRS) 1 and PRS 2 cDNAs were utilized as probes to localize the corresponding human PRPS1 and PRPS2 genes, previously reported to be X chromosome linked. PRPS1 and PRPS2 loci mapped to the intervals Xq22-q24 and Xp22.2-p22.3, respectively, using a combination of in situ chromosomal hybridization and human x rodent somatic cell panel genomic DNA hybridization analyses. A PRPS1-related gene or pseudogene (PRPS1L2) was also identified using in situ chromosomal hybridization at 9q33-q34. Human HPRT and PRPS1 loci are not closely linked. Despite marked cDNA and deduced amino acid sequence homology, human PRS 1 and PRS 2 isoforms are encoded by genes widely separated on the X chromosome.
Becker
M A
MA
Department of Medicine, University of Chicago, Illinois 60637.
Heidler
S A
SA
Bell
G I
GI
Seino
S
S
Le Beau
M M
MM
Westbrook
C A
CA
Neuman
W
W
Shapiro
L J
LJ
Mohandas
T K
TK
Roessler
B J
BJ
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D005796:Genes; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D009693:Nucleic Acid Hybridization; D012268:Ribose-Phosphate Pyrophosphokinase / Q000235:genetics*; D014960:X Chromosome
D004247:DNA; D012268:Ribose-Phosphate Pyrophosphokinase