Defective production of interleukin 6 by macrophages from C3H/HeJ mice: differential stimulation of interleukin 1 and interleukin 6 induction.
Resident macrophages from lipopolysaccharide (LPS)-hyporesponsive C3H/HeJ mice, in contrast with macrophages from other mouse strains, produced neither interleukin 6 (IL-6) nor interleukin 1 (IL-1) spontaneously. The stimulation of C3H/HeJ macrophages with poly(I:C), LPS or, to a lesser extent, muramyl dipeptide (MDP), induced the production of both cytokines. High levels of intracellular IL-1 activity were produced which were not released in surrounding media unless silica was added to stimulated cells. On the other hand, IL-6 was secreted in the absence of any additional stimulus, hence also in the absence of extracellular IL-1. The kinetics of IL-1 and IL-6 production by LPS-stimulated macrophages were studied. Intracellular IL-1 produced by stimulated C3H/HeJ macrophages reached the levels produced by macrophages from C3H/HeN mice. IL-6-induced secretion, however, was about 5-fold less important than in C3H/HeN macrophages, suggesting a quantitative defect rather than an intrinsic defect in IL-6 production.
Sarih
M
M
C.N.R.S., URA 1116, Institut de Biochimie, Université Paris-Sud, Orsay, France.
Souvannavong
V
V
Adam
A
A
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology; D000818:Animals; D001681:Biological Assay; D005260:Female; D007375:Interleukin-1 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D007700:Kinetics; D008070:Lipopolysaccharides; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D010529:Peritoneal Cavity / Q000166:cytology; D011070:Poly I-C / Q000494:pharmacology
D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D011070:Poly I-C