Lack of sensitivity of sister-chromatid exchange for lymphocyte chromosomal damage detection caused by antichagasic treatment.
No studies exist on sister-chromatid exchange (SCE) formation in chagasic patients therapeutically exposed to nifurtimox (NFX) or benznidazol (BZ). In the present study SCE was analyzed in cultures of peripheral lymphocytes of patients aged 11 months to 11 years treated with NFX 12-15 mg/kg/d for 60 days or with BZ 5 mg/kg/d for 30 days. Chagasic patients before treatment constituted a control group. A mean of 30 metaphases were examined for each individual. All treated patients compared with untreated controls did not show a significant increase in SCE frequency. Compared with the percentage of chromosomal aberrations in these patients and others belonging to the same epidemic protocol, SCE seems to be less sensitive in the detection of lymphocyte chromosomal damage caused by NFX or BZ.
Gorla
N B
NB
Sección Citogenética, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, Argentina.
Ledesma
O S
OS
Barbieri
G P
GP
Larripa
I B
IB
D014355:Chagas Disease / Q000097:blood / Q000188:drug therapy* / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D002875:Chromosomes / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009547:Nifurtimox / Q000009:adverse effects* / Q000627:therapeutic use; D009593:Nitroimidazoles / Q000009:adverse effects* / Q000627:therapeutic use; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D014344:Trypanocidal Agents / Q000009:adverse effects* / Q000627:therapeutic use
D009593:Nitroimidazoles; D014344:Trypanocidal Agents; D009547:Nifurtimox; C009999:benzonidazole