Low concentrations of interferon-gamma stimulate DNA synthesis in normal human keratinocytes during the initial stages of cultivation.
High concentrations of interferons can inhibit keratinocyte proliferation and induce the expression of HLA-DR antigen on keratinocytes. In the present study, the effects of low concentrations of recombinant human interferon alpha, beta and gamma were examined on DNA synthesis and the expression of HLA-DR and bullous pemphigoid (BP) antigens in normal human keratinocytes. Low concentrations of interferons induced DNA synthesis in normal human keratinocytes when epidermal cell growth factor and low Ca++ were used during the initial stages of culturing. This result indicates that physiological concentrations of interferons can induce keratinocyte activation.
Okubo
K
K
Department of Dermatology, Kurume University School of Medicine, Japan.
Karashima
T
T
Hachisuka
H
H
Sasai
Y
Y
D000818:Animals; D001324:Autoantigens / Q000032:analysis; D002352:Carrier Proteins; D002478:Cells, Cultured; D003094:Collagen; D003598:Cytoskeletal Proteins; D004247:DNA / Q000096:biosynthesis*; D000071821:Dystonin; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D015603:Keratinocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D011817:Rabbits; D000097805:Collagen Type XVII
D001324:Autoantigens; D002352:Carrier Proteins; D003094:Collagen; D003598:Cytoskeletal Proteins; D004247:DNA; D000071821:Dystonin; D006684:HLA-DR Antigens; D007371:Interferon-gamma; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D000097805:Collagen Type XVII; C486268:DST protein, human