title: Autoradiographical analysis of excitatory amino acid binding sites in rat hippocampus during the development of hippocampal kindling.
abstract: Binding sites for excitatory amino acids have been determined by autoradiographical procedures in the rat hippocampus and striatum during hippocampal kindling. The binding sites measured were the N-methyl-D-aspartate (NMDA)-sensitive sites for L-[3H]glutamate and [3H]MK-801 sites (transmitter recognition site and ion channel of the NMDA receptor, respectively), [3H]alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) sites (quisqualate receptor), [3H]kainate sites (kainate receptor) and NMDA-insensitive sites for L-[3H]glutamate. In general, little change was apparent in the hippocampus or striatum for any of these binding sites when assessed 48 h after attaining stages 1/2, 3 or 5 of kindling. These results suggest that hippocampal kindling does not bring about a change in the excitatory amino acid receptor binding sites examined, and that the appearance of an NMDA receptor-mediated component to synaptic responses in the hippocampus produced by kindling, cannot be explained on this basis.
authors.affiliation: Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Vezzani
authors.forename: R
authors.initials: R
authors.lastname: Serafini
authors.forename: R
authors.initials: R
authors.lastname: Samanin
authors.forename: A C
authors.initials: AC
authors.lastname: Foster
mesh_terms: D000596:Amino Acids; D000818:Animals; D001345:Autoradiography; D004558:Electric Stimulation; D006624:Hippocampus / Q000737:chemistry*; D007608:Kainic Acid; D007696:Kindling, Neurologic / Q000378:metabolism*; D008297:Male; D010069:Oxadiazoles; D051381:Rats; D011919:Rats, Inbred Strains; D018091:Receptors, AMPA; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface / Q000032:analysis*; D018092:Kainic Acid Receptors; D016194:Receptors, N-Methyl-D-Aspartate / Q000032:analysis; D017981:Receptors, Neurotransmitter / Q000032:analysis; D014316:Tritium
chemical_list: D000596:Amino Acids; D007608:Kainic Acid; D010069:Oxadiazoles; D018091:Receptors, AMPA; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface; D018092:Kainic Acid Receptors; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D014316:Tritium