Identification of delta 5,7-sterol-delta 7-reductase in higher plant microsomes.
A microsomal preparation from seedlings of Zea mays catalyzed the NADPH dependent reduction of the delta 7-bond of delta 5,7-cholestadienol (1) giving the first in vitro evidence for the intermediacy of delta 5,7-sterols in plant sterol biosynthesis. Using a GC assay developed to detect the cholesterol (2) produced, the properties of the microsomal enzyme have been established with respect to cofactor requirements and kinetics. The potent in vitro inhibition of the plant delta 5,7-sterol-delta 7-reductase by the ammonium-ion containing fungicides, tridemorph2 (3), fenpropimorph (4) and AY 9944 (5) was demonstrated. The high affinities observed for these derivatives, especially for (4) (I50 = 8 x 10(-8) M, I50/Km = 2 x 10(-4)), are in full accordance with the previously proposed cationic mechanism involved in this reduction reaction.
Taton
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Departement d'Enzymologie Moléculaire et Cellulaire de l'IBMP, CNRS UPR 406, Institut de Botanique, Strasbourg, France.
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D002784:Cholesterol / Q000302:isolation & purification / Q000378:metabolism; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D012639:Seeds / Q000201:enzymology; D013261:Sterols / Q000494:pharmacology; D003313:Zea mays / Q000201:enzymology*
D013261:Sterols; C033144:lathosterol delta-5-dehydrogenase; D002784:Cholesterol; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors