title: Increased variation in ADH enzyme activity in Drosophila mutation-accumulation experiment is not due to transposable elements at the Adh structural gene.
abstract: We present here a molecular analysis of the region surrounding the structural gene encoding alcohol dehydrogenase (Adh) in 47 lines of Drosophila melanogaster that have each accumulated mutations for 300 generations. While these lines show a significant increase in variation of alcohol dehydrogenase enzyme activity compared to control lines, we found no restriction map variation in a 13-kb region including the complete Adh structural gene and roughly 5 kb of both 5' and 3' sequences. Thus, the rapid accumulation of ADH activity variation after 28,200 allele generations does not appear to have been due to the mobilization of transposable elements into or out of the Adh structural gene region.
authors.affiliation: Laboratory of Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
authors.forename: C F
authors.initials: CF
authors.lastname: Aquadro
authors.forename: H
authors.initials: H
authors.lastname: Tachida
authors.forename: C H
authors.initials: CH
authors.lastname: Langley
authors.forename: K
authors.initials: K
authors.lastname: Harada
authors.forename: T
authors.initials: T
authors.lastname: Mukai
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D014644:Genetic Variation; D009154:Mutation; D015183:Restriction Mapping
chemical_list: D004251:DNA Transposable Elements; D000426:Alcohol Dehydrogenase