title: Interactions between the human monocytic leukaemia THP-1 cell line and Old and New World species of Leishmania.
abstract: The human promyelocytic THP-1 cell line has been found to support the growth of Leishmania parasites. THP-1 cells, differentiated with retinoic acid, cease replication while remaining in suspension. 72 +/- 8% of THP-1 cells became infected after inoculation with promastigotes of several Old and New World Leishmania species. The resulting amastigotes (19 +/- 5 per infected cell) were easy to harvest, capable of reinfecting cultures of normal human cells and, in the case of L. major and L. infantum, caused specific lesions in BALB/c mice. This culture system should facilitate biochemical and immunological studies on amastigotes and be of use in screening anti-parasite drugs.
authors.affiliation: INSERM U313, CHU Pitie-Salpêtrière, Paris, France.
authors.forename: B W
authors.initials: BW
authors.lastname: Ogunkolade
authors.forename: I
authors.initials: I
authors.lastname: Colomb-Valet
authors.forename: L
authors.initials: L
authors.lastname: Monjour
authors.forename: A
authors.initials: A
authors.lastname: Rhodes-Feuillette
authors.forename: J P
authors.initials: JP
authors.lastname: Abita
authors.forename: D
authors.initials: D
authors.lastname: Frommel
mesh_terms: D000818:Animals; D006801:Humans; D007891:Leishmania / Q000254:growth & development* / Q000502:physiology; D007893:Leishmania donovani / Q000254:growth & development / Q000502:physiology; D007895:Leishmania tropica / Q000254:growth & development / Q000502:physiology; D007951:Leukemia, Myeloid; D016403:Lymphoma, Large B-Cell, Diffuse; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000469:parasitology*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D014212:Tretinoin