Krabbe disease locus mapped to chromosome 14 by genetic linkage.
Using genetic linkage we have localized the gene coding for galactocerebrosidase (GALC) to human chromosome 14. Patients with Krabbe disease and their family members were assayed for GALC activity in leukocytes or fibroblasts and were classified as affected, carrier, noncarrier, or unknown. Polymorphic DNA markers from chromosome 14 demonstrated a multipoint LOD score of 3.40 with GALC located 13 cM centromere distal to CRI-C70 (D14S24). This finding is consistent with the location of the mouse twitcher mutation (a model of human GALC deficiency) on chromosome 12, which has substantial homology to human chromosome 14. Our data do not support a previous report's localization of GALC to chromosome 17.
Zlotogora
J
J
Department of Biochemistry and Molecular Biology, Jefferson Medical College, Philadelphia, PA 19107.
Chakraborty
S
S
Knowlton
R G
RG
Wenger
D A
DA
D002883:Chromosomes, Human, Pair 14; D005260:Female; D005698:Galactosylceramidase / Q000172:deficiency / Q000235:genetics; D006580:Genetic Carrier Screening; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D007965:Leukodystrophy, Globoid Cell / Q000235:genetics*; D008297:Male; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D005819:Genetic Markers; D005698:Galactosylceramidase