Characterization of haemagglutinin from Bordetella bronchiseptica.
In comparison with haemagglutinin (HA)-active strains of Bordetella bronchiseptica, the HA-deficient strains lacked a 150 kDa protein band on SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Adsorption of partially purified HA with bovine erythrocytes showed the loss of the 150 kDa band in the supernatant. This 150 kDa protein was purified by high-performance liquid chromatography using gel filtration columns. Electron microscopic examination revealed that the purified HA possessed a fine filamentous structure with dimensions of approximately 2 x 150 nm, which was considered to represent the filamentous haemagglutinin of B. bronchiseptica. Both the cell-free antigen obtained from the culture supernatant of phase-I strain of the bacteria and the bovine erythrocytes, which combined with crude HA showed an excellent protective activity of the challenge by virulent strain of B. bronchiseptica in mice.
Ohgitani
T
T
Division of Veterinary Microbiology, Kyoto Biken Laboratories, Japan.
Okabe
T
T
Sasaki
N
N
D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification; D016950:Bordetella bronchiseptica / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006388:Hemagglutinins / Q000276:immunology / Q000302:isolation & purification*; D008297:Male; D051379:Mice
D000907:Antibodies, Bacterial; D006388:Hemagglutinins