The DNA sequence specificity of cyanomorpholinoadriamycin.
Cyanomorpholinoadriamycin (1 microM) was reacted in a transcription buffer with DNA of an initiated transcription complex. Subsequent elongation of the initiated complex revealed permanent transcriptional blockages at 16 sites after only 5 min of drug-DNA reaction time. The most dominant sites were immediately prior to 5'-CC (six) and 5'-GG (six) sequences of the non-template strand, consistent with the presence of intrastrand crosslinking between adjacent guanine residues. Minor levels of blockage were at 5'-GC and 5'-CG sequences and may reflect low levels of interstrand crosslinking.
Cullinane
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Department of Biochemistry, La Trobe University, Bundoora, Victoria, Australia.
Phillips
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D001483:Base Sequence; D003432:Cross-Linking Reagents; D004247:DNA / Q000302:isolation & purification*; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D007700:Kinetics; D008969:Molecular Sequence Data; D014158:Transcription, Genetic
D003432:Cross-Linking Reagents; D004317:Doxorubicin; C038555:3'-deamino-3'-(3-cyano-4-morpholinyl)doxorubicin; D004247:DNA