The detection of antibodies to the glycoprotein X antigen of pseudorabies virus.
The persistence of antibodies to glycoprotein X (gpX) in the serum of pigs experimentally infected with pseudorabies virus (PRV) was determined using an anti-gpX enzyme-linked immunosorbent assay (ELISA). Antibodies to gpX were detected for at least 365 days postchallenge in nonvaccinated pigs. Previous sensitization of pigs by vaccination with S/PRV had no apparent effect on the antibody response of pigs to gpX postchallenge. In determining previous exposure of pigs to PRV strains containing the gpX gene, the anti-gpX ELISA was highly specific, but its sensitivity was lower than the standard serological procedures currently used for detecting PRV antibodies.
Cook
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D
Veterinary Diagnostic Laboratory, Iowa State University, Ames 50010.
Hill
H
H
Snyder
M
M
McMahon
P
P
Kinker
D
D
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D009500:Neutralization Tests; D011237:Predictive Value of Tests; D011557:Pseudorabies / Q000276:immunology*; D015531:Radioimmunoprecipitation Assay; D013552:Swine; D013553:Swine Diseases / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary; D014759:Viral Envelope Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology
D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C042966:pseudorabies virus glycoproteins