The kinetics of electron entry in cytochrome c oxidase.
The kinetics of electron entry in beef heart cytochrome c oxidase have been studied by stopped-flow spectroscopy following chemical modification of the CuA site with mercurials. In this derivative CuA is no longer reducible by cytochrome c while cytochrome alpha may accept electrons from the latter with rates comparable to the native enzyme. The results indicate that CuA is not the exclusive electron entry site in cytochrome c oxidase.
Malatesta
F
F
Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Universitá di Roma Tor Vergata, Italy.
Antonini
G
G
Sarti
P
P
Vallone
B
B
Brunori
M
M
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D003300:Copper / Q000378:metabolism; D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D009206:Myocardium / Q000201:enzymology*; D010084:Oxidation-Reduction
D003300:Copper; D003576:Electron Transport Complex IV