[Immunogenetic mechanism of Behçet's disease].
In order to investigate the immunogenetic mechanism of Behçet's disease, frequencies of HLA antigens were studied in patients. The subjects consisted of 66 patients and 99 normal controls. A lymphocyte cytotoxicity test was used for typing HLA-A, -B, -C, -DR, -DQ antigens. HLA-DP antigens were analyzed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. A significant increase of HLA-B15 was observed in the patients. In contrast, no significant difference was observed in HLA-Bw52 which possesses only two different amino acids from HLA-B15. On the contrary, frequencies of HLA-A11, HLA-Aw33, HLA-B35, HLA-B44 and HLA-DQw1 were significantly lower in the patients than in the controls. No significant difference was observed in HLA-DP antigens. These results suggest that Behçet's disease involves both disease susceptibility factors and disease resistance factors and that such genetic factors are mapped within or very close to the HLA-B gene in the class I gene region. Additionally class II HLA-DQ antigen is associated with disease resistance factors.
Mizuki
N
N
Department of Ophthalmology, Yokohama City University School of Medicine, Japan.
Ohno
S
S
Kamata
K
K
Nakamura
S
S
Ishihara
M
M
Sato
K
K
Inaba
G
G
Tsuji
K
K
Inoko
H
H
D000595:Amino Acid Sequence; D001528:Behcet Syndrome / Q000235:genetics*; D002874:Chromosome Mapping; D005260:Female; D006680:HLA Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics; D006683:HLA-DQ Antigens / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data
D006680:HLA Antigens; D015235:HLA-B Antigens; D006683:HLA-DQ Antigens