On the action of hypochlorite on human serum albumin.
The luminol-enhanced chemiluminescence of zymosan-stimulated polymorphonuclear leukocytes is continuously diminished in the presence of increasing amounts of human serum albumin (from 1 to 30 mg/ml). HSA competes with luminol for hypochlorite as shown by adding sodium hypochlorite to luminol solutions containing HSA. Titration of HSA with NaOCl affects firstly the sulfhydryl and probably the thioether groups and then the amino groups. Coincubation of zymosan-stimulated polymorphonuclear leukocytes and HSA at a ratio corresponding to normal values of healthy men causes a continuous oxidation of sulphur containing functional groups in HSA.
Arnhold
J
J
Institute of Biophysics, School of Medicine, Karl Marx University Leipzig, GDR.
Hammerschmidt
S
S
Wagner
M
M
Mueller
S
S
Arnold
K
K
Grimm
E
E
D005453:Fluorescence; D006801:Humans; D006997:Hypochlorous Acid / Q000494:pharmacology*; D008163:Luminescent Measurements; D008165:Luminol; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D009195:Peroxidase / Q000097:blood*; D012709:Serum Albumin / Q000378:metabolism*; D013438:Sulfhydryl Compounds; D015054:Zymosan / Q000494:pharmacology
D012709:Serum Albumin; D013438:Sulfhydryl Compounds; D008165:Luminol; D006997:Hypochlorous Acid; D015054:Zymosan; D009195:Peroxidase