[Establishment and characterization of a monoclonal antibody against egg antigens of Schistosoma japonicum].
A monoclonal antibody (N29B3) against egg antigens of Schistosoma japonicum was obtained by hybridoma technique N29B3 was of IgG3 isotype. The molecular weight of its target antigen was 141 KD. IIFA with section of liver from infected rabbit showed that N29B3 was located on surface of miracidium and egg shell. IIFA and ELISA also showed that N29B3 was not reactive with antigens of differential developmental stages of S. japonicum and antigens of other trematodes. The data indicated that N29B3 has specificity for egg antigens. In western blotting, sera from chronic infected human and mice and from acute infected mice (30 and 45 days) all recognized 141 KD molecule. These results suggest that 141 KD molecule has potential capacity for early diagnosis of schistosomiasis.
Wang
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D000947:Antigens, Helminth / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010063:Ovum / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth