Molecular and genetic characterization of human cell lines resistant to L-asparaginase and albizziin.
Human cell lines resistant to L-asparaginase or albizziin were isolated by multistep selection of HT1080 fibrosarcoma and MIA PaCa-2 pancreatic carcinoma cells. Mutants were cross-resistant to both drugs, but more resistant to the drug used for selection. The drug-resistant cell lines expressed elevated levels of asparagine synthetase activity and protein, up to 17-fold over that of the parental cells. Enzyme overproduction was due to gene amplification in the albizziin-resistant cells, whereas increased expression without amplification was observed in L-asparaginase-resistant cells.
Andrulis
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Mount Sinai Hospital Research Institute, Toronto, Ontario, Canada.
Argonza
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Cairney
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AE
D000599:Amino Acids, Diamino / Q000494:pharmacology*; D001215:Asparaginase / Q000494:pharmacology*; D001217:Aspartate-Ammonia Ligase / Q000096:biosynthesis / Q000235:genetics*; D002460:Cell Line; D004351:Drug Resistance / Q000235:genetics; D005784:Gene Amplification; D006801:Humans; D008025:Ligases / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D000599:Amino Acids, Diamino; D012333:RNA, Messenger; D001215:Asparaginase; D008025:Ligases; D001217:Aspartate-Ammonia Ligase; C006440:albizziin