Interleukin-1 beta induces interleukin-1 alpha messenger ribonucleic acid expression in primary cultures of Leydig cells.
Previously, we have reported that interleukin-1 (IL-1) can modulate Leydig cell steroidogenesis. Recently, IL-1-like material has been shown to be present in the testis; however, the cellular source of this material remains unclear. In the present study we found that human recombinant IL-1 beta (1-100 ng/ml) caused dose-dependent increases in IL-1 alpha mRNA expression in Leydig cells. Similar to that reported in other tissues, IL-1 alpha mRNA from Leydig cells is mainly 2.2 kilobases. IL-1 alpha mRNA expression in Leydig cells was detectable as early as 2 h after the addition of IL-1 beta (10 ng/ml) and persisted for up to 24 h. Lipopolysaccharide also stimulated IL-1 alpha mRNA expression in these cells, but phorbol ester had no effect. Our results indicate that Leydig cells are a potential source of IL-1, which has both autocrine and paracrine effects.
Wang
D L
DL
Medical Service, W. J. B. Dorn Veterans Hospital, Columbia, South Carolina 29201.
Nagpal
M L
ML
Calkins
J H
JH
Chang
W W
WW
Sigel
M M
MM
Lin
T
T
D000199:Actins / Q000235:genetics; D000818:Animals; D002478:Cells, Cultured; D004926:Escherichia coli; D015870:Gene Expression; D007375:Interleukin-1 / Q000235:genetics / Q000494:pharmacology*; D007700:Kinetics; D007985:Leydig Cells / Q000378:metabolism*; D008070:Lipopolysaccharides; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000494:pharmacology
D000199:Actins; D007375:Interleukin-1; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D011994:Recombinant Proteins