Interactions of antisense peptides with ovine prolactin.
Two antisense peptides were synthesized to a sense peptide corresponding to amino acid residues 23-35 of ovine prolactin. Both of the antisense peptides formed a saturable complex with the sense peptide and ovine prolactin. The sense peptide inhibited the interaction of ovine prolactin with the antisense peptides. Both of the antisense peptides have a common core sequence VMNV which can bind to ovine prolactin. The lactogenic hormones, rat prolactin and human growth hormone, compete with the binding of ovine prolactin to an antisense peptide whereas a nonlactogen, ovine growth hormone, does not compete indicating a degree of specificity in the interaction.
Bajpai
A
A
Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City 66103.
Hooper
K P
KP
Ebner
K E
KE
D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay; D013006:Growth Hormone / Q000494:pharmacology; D007106:Immune Sera; D007700:Kinetics; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000378:metabolism*; D011388:Prolactin / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012756:Sheep
D007106:Immune Sera; D009842:Oligopeptides; D011388:Prolactin; D013006:Growth Hormone