Enzyme immunoassay of human plasma 11-dehydrothromboxane B2.
11-Dehydrothromboxane B2 (11-dhTXB2) is a proposed marker compound for thromboxane A2 formed in vivo. An enzyme immunoassay was established for determination of the plasma concentration of this compound. The assay was based on a horseradish peroxidase-linked immunoassay utilizing polyclonal anti-11-dhTXB2 antibody obtained from a rabbit, and enabled determination of 11-dhTXB2 in the range of 2 to 500 pg/tube with an IC50 of 36 pg. The cross-reactivities with TXB2, 2,3-dinor-TXB2 and other prostanoids were less than 0.05%. Validity of the enzyme immunoassay was confirmed by a radioimmunoassay utilizing a monoclonal antibody. The plasma 11-dhTXB2 was immunoaffinity-purified by one step using an immobilized monoclonal antibody. The mean plasma level of 11-dhTXB2 in six male volunteers was 4.0 +/- 0.3 pg/ml by this enzyme immunoassay.
Takasaki
W
W
Analytical and Metabolic Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan.
Nakagawa
A
A
Tanaka
Y
Y
Nakamura
K
K
Shindo
H
H
Hayashi
Y
Y
Yamamoto
S
S
D015415:Biomarkers; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D011863:Radioimmunoassay; D013929:Thromboxane B2 / Q000031:analogs & derivatives* / Q000097:blood
D015415:Biomarkers; D013929:Thromboxane B2; C049235:11-dehydro-thromboxane B2