A new genetic polymorphism in human platelet polypeptides detected by two-dimensional electrophoresis.
We described a new genetic polymorphism of human platelet polypeptide, detected by two-dimensional polyacrylamide gel electrophoresis followed by silver-staining. The polymorphism was tentatively designated thrombocyte B (ThB) with a molecular weight of 34 kDa and isoelectric point of 4.7-4.8. In this polypeptide, three different electrophoretic types (1-1, 1-2, 2-2) were identified. Family and population studies indicate that the three phenotypes of the polypeptide are determined by two common alleles at a single autosomal locus. In a Japanese population, the gene frequency of ThB 1/ThB 2 was 0.74/0.26. The ThB polypeptide was not found in other blood cells and decreased or disappeared during the preparation or storage of platelets.
Kajii
E
E
Department of Legal Medicine and Human Genetics, Jichi Medical School, Tochigi, Japan.
Iwamoto
S
S
Omi
T
T
Ikemoto
S
S
D000483:Alleles; D001792:Blood Platelets / Q000737:chemistry*; D001798:Blood Proteins / Q000235:genetics*; D003196:Computer Graphics; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007564:Japan; D008297:Male; D010375:Pedigree; D010455:Peptides / Q000097:blood / Q000235:genetics*; D010641:Phenotype; D011110:Polymorphism, Genetic
D001798:Blood Proteins; D010455:Peptides