title: Polyalkylenes used as stationary phases for preparative reversed-phase liquid chromatography.
abstract: Polyethylene and polypropylene were employed as stationary phases for preparative liquid chromatography and compared with LiChroprep RP-18. Polyalkylene phases are chemically stable and have homogeneous hydrophobic surfaces, but they are physically less resistant than silica-based phases. The influence of organic modifiers and salt ions on the capacity factors of aromatic hydrocarbons and peptides was determined. Peptides prepared by solid-phase peptide synthesis were purified on polypropylene. The results demonstrate the usefulness of polyalkylenes in preparative reversed-phase liquid chromatography, particularly for basic peptides.
authors.affiliation: Institute of Drug Research, Academy of Sciences, Berlin, G.D.R.
authors.forename: E
authors.initials: E
authors.lastname: Krause
authors.forename: D
authors.initials: D
authors.lastname: Smettan
authors.forename: F
authors.initials: F
authors.lastname: Loth
authors.forename: H
authors.initials: H
authors.lastname: Herma
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006838:Hydrocarbons; D010455:Peptides / Q000032:analysis; D011095:Polyethylenes; D011126:Polypropylenes; D012822:Silicon Dioxide
chemical_list: D006838:Hydrocarbons; D010455:Peptides; D011095:Polyethylenes; D011126:Polypropylenes; D012822:Silicon Dioxide