Demonstration of clonality in T-cell lymphoma using an anti-T-cell receptor variable region antibody panel.
Frozen sections from 35 T-cell lymphomas were stained with the Diversi-T alpha beta T-Cell Receptor panel which includes seven antibodies to T-cell receptor variable region gene products. In five cases a monoclonal population of T-cells could be demonstrated (one case V beta 5+, three cases V beta 8+ and one case V beta 6+) and in a further case a biclonal population (V beta 5+ and V beta 8+). We conclude that this antibody panel is of limited usefulness for the demonstration of clonality in T-cell lymphoma.
O'Grady
J
J
Department of Pathology, University Medical School, Edinburgh, Scotland, UK.
Krajewski
A S
AS
Ramage
E F
EF
D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006801:Humans; D007135:Immunoglobulin Variable Region; D007150:Immunohistochemistry; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000473:pathology*
D007135:Immunoglobulin Variable Region