title: Location of the three major agglutinogens of Bordetella pertussis by immuno-electronmicroscopy.
abstract: When the three serotypes of Bordetella pertussis (types 1,2,3; 1,2 and 1,3) were labelled with agglutinins and protein-A gold, agglutinogen 1 was found on fimbriae and on the cell surface of types 1,2,3 and 1,2 but on the cell surface only of non-fimbriate type 1,3 organisms. In contrast, agglutinogen 2 was located on fimbriae only. Agglutinogen 3 was not labelled. When protein-A gold was replaced by immunoglobulin-G gold, agglutinogen 3 was found on the cell surface only, even of fimbriate bacteria of type 1,2,3. The implications of these findings for acellular vaccines are discussed.
authors.affiliation: Pertussis Reference Laboratory, Department of Medical Microbiology, University Medical School, Manchester.
authors.forename: N W
authors.initials: NW
authors.lastname: Preston
authors.forename: A A
authors.initials: AA
authors.lastname: Zorgani
authors.forename: E J
authors.initials: EJ
authors.lastname: Carter
mesh_terms: D000372:Agglutination Tests; D000373:Agglutinins / Q000032:analysis*; D000942:Antigens, Bacterial / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D001886:Bordetella pertussis / Q000032:analysis* / Q000648:ultrastructure; D010861:Fimbriae, Bacterial / Q000032:analysis*; D007150:Immunohistochemistry / Q000379:methods; D008854:Microscopy, Electron; D013045:Species Specificity
chemical_list: D000373:Agglutinins; D000942:Antigens, Bacterial; D000954:Antigens, Surface