Cytochemical demonstration of acid phosphatase, trimetaphosphatase and basic protein in rat peritoneal mast cells during 48/80 induced exocytosis.
Acid phosphatase (AcP-A), trimetaphosphatase (TmP-A) activities and basic protein reaction were cytochemically studied in rat peritoneal mast cells 15 minutes after stimulation by compound 48/80. The AcPase reaction was positive in slightly altered granules, but negative in those more intensely altered, and also in unaltered granules. The TmP-A reaction was negative in altered granules and positive in a few unaltered granules. These results suggest that mast cells have two populations of granules, one, comprising most granules, is AcP-A positive and is exocytosed. The other, smaller, is TmP-A positive, and is not exocytosed. Intact granules gave a strong positive reaction with amoniacal silver nitrate (AS), which detects basic protein. This reaction decreased in intensity with increasing granule alteration.
Jamur
M C
MC
Departamento de Biologia Celular, Universidade Federal do Paraná, São Paulo, Brasil.
Vugman
I
I
D017766:Acid Anhydride Hydrolases; D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D005089:Exocytosis; D005260:Female; D006863:Hydrogen-Ion Concentration; D008297:Male; D008407:Mast Cells / Q000201:enzymology / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D011506:Proteins; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases; D017766:Acid Anhydride Hydrolases; C022768:trimetaphosphatase