title: Inverse relationship of epidermal growth factor receptor and HER2/neu gene expression in human renal cell carcinoma.
abstract: Expression of the oncogenes, epidermal growth factor (EGF) receptor, HER2/neu, c-myc, and c-fos, in renal cell carcinoma and corresponding nonneoplastic kidney tissue of 30 patients has been analyzed by Northern blot analysis. In renal cell carcinoma an inverse relationship of EGF receptor and HER2/neu gene expression was detected, with high expression of the EGF receptor gene in 22 of 30 (73%) cases and low expression of the HER2/neu gene in 28 of 30 (93%) cases. Furthermore, altered expression of the oncogenes c-myc and c-fos was detected in renal cell carcinoma, which appears to be related to the tumor grade of malignancy. Additional Southern blot analysis of six renal cell carcinomas gave no indication of chromosomal rearrangement events or gene amplification.
authors.affiliation: Institut für Physiologische Chemie, University of Düsseldorf, West Germany.
authors.forename: U
authors.initials: U
authors.lastname: Weidner
authors.forename: S
authors.initials: S
authors.lastname: Peter
authors.forename: T
authors.initials: T
authors.lastname: Strohmeyer
authors.forename: R
authors.initials: R
authors.lastname: Hussnätter
authors.forename: R
authors.initials: R
authors.lastname: Ackermann
authors.forename: H
authors.initials: H
authors.lastname: Sies
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D015152:Blotting, Northern; D015139:Blotting, Southern; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D066246:ErbB Receptors / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D018719:Erb-b2 Receptor Tyrosine Kinases
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D012334:RNA, Neoplasm; D018719:Erb-b2 Receptor Tyrosine Kinases