[In vitro structural aspects of the human trophoblastic cell].
Maternal- fetal exchanges are mainly regulated by trophoblast, which displays an active role during embryo growth. Trophoblast organization into a syncytial layer involves structural and functional steps that may be monitored and better elucidated by "in vitro" studies. In light of this, we have carried out morphological and biochemical analyses in order to evaluate 1) the syncytiotrophoblast formation in culture (48 h, 5-30 days) the Na+/K+ATPase activity and 3) the plasmalemmal microviscosity changes occurring during "in vitro" trophoblast production. Morphological and biochemical modulations have been pointed out.
Pugnaloni
A
A
Istituto di Morfologia Umana Normale, Università di Ancona.
Faloia
E
E
Saccucci
F
F
Mazzanti
L
L
Solmi
R
R
Cester
N
N
Romanini
C
C
De Pirro
R
R
Biagini
G
G
D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008431:Maternal-Fetal Exchange; D008560:Membrane Fluidity; D011247:Pregnancy; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014327:Trophoblasts / Q000201:enzymology / Q000648:ultrastructure*
D000254:Sodium-Potassium-Exchanging ATPase
Aspetti strutturali "in vitro" delle cellule trofoblastiche umane.