Antiviral effect of 6-diazo-5-oxo-L-norleucine, antagonist of gamma-glutamyl transpeptidase, on replication of human parainfluenza virus type 2.
The antiviral effects of 6-diazo-5-oxo-L-norleucine (L-DON) on the replication of human parainfluenza virus type 2 (HPIV-2), mumps and vesicular stomatitis viruses were studied. L-DON suppressed growth of these viruses and, in particular, HPIV-2 in four cell types. L-DON was not toxic to the cells at the active dose and did not significantly inhibit cellular macromolecular synthesis. The L-DON-sensitive step of HPIV-2 replication was considered to be relatively early. The NP, P and M proteins were, although at a low level, clearly detectable in HPIV-2-infected Vero cells treated with L-DON, whereas the HN and F proteins were scarcely detected by either immunostaining or immunoprecipitation, indicating that L-DON mainly decreased the amounts of viral glycoproteins. Furthermore, Northern blot hybridization showed that secondary transcription of virus RNA was also inhibited.
Nishio
M
M
Department of Microbiology, Mie University School of Medicine, Mie-Prefecture, Japan.
Tsurudome
M
M
Bando
H
H
Komada
H
H
Ito
Y
Y
D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology*; D015152:Blotting, Northern; D002460:Cell Line; D003980:Diazooxonorleucine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005455:Fluorescent Antibody Technique; D006801:Humans; D009109:Mumps virus / Q000187:drug effects / Q000502:physiology; D010223:Parainfluenza Virus 2, Human / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D015531:Radioimmunoprecipitation Assay; D018108:Respirovirus / Q000187:drug effects*; D014709:Vero Cells; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000502:physiology; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000187:drug effects; D005723:gamma-Glutamyltransferase / Q000037:antagonists & inhibitors*
D001391:Azo Compounds; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D003980:Diazooxonorleucine; D005723:gamma-Glutamyltransferase