cDNA cloning and localization of the mouse leukosialin gene (Ly48) to chromosome 7.
Mouse leukosialin, previously known as the 3E8 antigen, is expressed primarily on cells of the hematopoietic and lymphoid lineages and is shown to be the mouse homologue to the human leukosialin/sialophorin and rat W3/13 molecules. A partial leukosialin cDNA clone was isolated via cross-species hybridization with a portion of a human leukosialin cDNA. This mouse cDNA clone was used to demonstrate that the leukosialin isoforms are encoded by a single mRNA species of approximately 4.2 kilobases (kb) and that the leukosialin gene is located on chromosome 7. Based on these results, mouse leukosialin is given the designation Ly48.
Baecher
C M
CM
Cancer Center Immunology Unit, University of Rochester Medical Center, NY 14642.
Dorfman
K S
KS
Mattei
M G
MG
Frelinger
J G
JG
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003429:Cross Reactions; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D051917:Leukosialin; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D012795:Sialoglycoproteins / Q000235:genetics*; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism
D015703:Antigens, CD; D051917:Leukosialin; D012333:RNA, Messenger; C465014:SPN protein, human; D012795:Sialoglycoproteins; C497863:Spn protein, mouse; C497864:Spn protein, rat; D011061:Poly A