[Detection of anti-cytomegalovirus antibodies: comparison of 3 commercial methods].
A group of 188 sera from pregnant women was screened for cytomegalovirus (CMV) antibodies by immunofluorescence assay (IFA), enzyme immunoassay (EIA), and passive latex agglutination (LA) tests. There were 178 (94.6%) sera for which there was complete agreement between the three tests. One hundred-sixty three sera were positive by two or more methods and judged to be true positives. Accordingly, the highest sensitivity was shown by the EIA and LA tests (100% in both cases). The sensitivity of IFA test was similar (97%). A ranking for technical demand, hands-on time, turnaround time, requirement for special equipment and objectivity sharply favoured the LA test.
García-Aguayo
J M
JM
Laboratorio de Microbiología, Hospital del SVS. Requena, Valencia.
D000372:Agglutination Tests; D000914:Antibodies, Viral / Q000032:analysis*; D003587:Cytomegalovirus / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D011237:Predictive Value of Tests; D011247:Pregnancy / Q000276:immunology*; D011933:Reagent Kits, Diagnostic
D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic
Detección de anticuerpos anti-citomegalovirus: comparación de tres métodos comerciales.