title: Retroviral expressed hemagglutinin-neuraminidase protein protects chickens from Newcastle disease virus induced disease.
abstract: The hemagglutinin-neuraminidase (HN) gene and the phosphoprotein (P) gene of Newcastle disease virus (NDV) were inserted into a replication competent avian leukosis virus vector. The expression of the HN gene from this vector in chick embryo cells has been previously reported. The P gene is also expressed from this vector in chick embryo cells. The retroviruses were used to immunize 4-week-old chickens. Birds receiving the virus containing the HN gene developed low levels of serum HI titers and NDV neutralization titers. Upon challenge, all birds vaccinated with the HN gene containing virus were protected from disease but not viral infection and replication. In contrast, birds immunized with the P gene containing retrovirus developed more severe clinical signs of disease earlier than birds receiving no immunization or retrovirus alone. The results obtained with the HN gene may have potential application to reducing disease due to NDV genetically engineered vaccines.
authors.affiliation: Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655.
authors.forename: T
authors.initials: T
authors.lastname: Morrison
authors.forename: V S
authors.initials: VS
authors.lastname: Hinshaw
authors.forename: M
authors.initials: M
authors.lastname: Sheerar
authors.forename: A J
authors.initials: AJ
authors.lastname: Cooley
authors.forename: D
authors.initials: D
authors.lastname: Brown
authors.forename: C
authors.initials: C
authors.lastname: McQuain
authors.forename: L
authors.initials: L
authors.lastname: McGinnes
mesh_terms: D000818:Animals; D000917:Antibody Formation; D001354:Avian Leukosis Virus / Q000235:genetics*; D002645:Chickens; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005814:Genes, Viral; D005822:Genetic Vectors; D015707:HN Protein / Q000235:genetics* / Q000276:immunology; D009521:Newcastle Disease / Q000276:immunology / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000235:genetics / Q000276:immunology; D010750:Phosphoproteins / Q000235:genetics* / Q000276:immunology; D010957:Plasmids; D011200:Poultry; D014162:Transfection; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics*
chemical_list: D015707:HN Protein; C055691:P phosphoprotein, Newcastle disease virus; D010750:Phosphoproteins; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines