Role of polyamines in the transport in vitro of the precursor of ornithine transcarbamylase.
Polyamines induce the transport in vitro of the rat liver precursor of ornithine transcarbamylase (pOTC) into isolated rat liver mitochondria. The accumulation of this precursor at the level of binding to the mitochondrial surface has allowed us to establish that polyamines are involved in the interaction of the precursor with the mitochondrial surface. Transport of a chimeric protein having the signal sequence of pOTC fused to a fragment of the cytosolic protein human arginosuccinate lyase was also induced by polyamines. The sensitivity of the pOTC synthesized in vitro and of the chimeric protein to proteinases decreases in the presence of polyamines. This result suggests that polyamines may play a role in modulating the folding of precursors to favour their binding to mitochondria.
González-Bosch
C
C
Instituto de Investigaciones Citológicas de la Caja de Ahorros de Valencia (Centro Asociado del CSIC), Spain.
Marcote
M J
MJ
Hernández-Yago
J
J
D000818:Animals; D001665:Binding Sites; D001692:Biological Transport / Q000187:drug effects; D007425:Intracellular Membranes / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology*; D009954:Ornithine Carbamoyltransferase / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D011073:Polyamines / Q000494:pharmacology*; D011487:Protein Conformation; D011498:Protein Precursors / Q000187:drug effects / Q000378:metabolism*; D021382:Protein Sorting Signals / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D010455:Peptides; D011073:Polyamines; D011498:Protein Precursors; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D009954:Ornithine Carbamoyltransferase