Production of anti-platelet-activating factor antibodies by the use of colloidal gold as carrier.
Polyclonal and monoclonal antibodies to platelet-activating factor (PAF) were produced, by use of colloidal gold as the hapten carrier. These polyclonal (R88-09) and monoclonal (602 B11) antibodies both reacted with PAF, lysoplatelet-activating factor (lyso PAF), and L-alpha-lysophosphatidylcholine, palmitoyl (lyso PCP), but did not react with phosphorylcholine chloride (PCC). Their affinities were higher for PAF than lyso PAF and lyso PCP.
Tomii
A
A
Department of Medicine and Geriatrics, Osaka University Medical School, Japan.
Masugi
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000917:Antibody Formation / Q000187:drug effects*; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006046:Gold; D006241:Haptens / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D010972:Platelet Activating Factor / Q000276:immunology*; D011817:Rabbits
D000911:Antibodies, Monoclonal; D006241:Haptens; D010972:Platelet Activating Factor; D006046:Gold