Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum.
The lysC/asd gene cluster of Corynebacterium glutamicum ATCC 13032 was cloned and sequenced. The lysC locus coding for aspartokinase consists of two in-frame overlapping genes, lysC alpha encoding a protein of 421 amino acids (Mr 44,300) and lysC beta encoding a protein of 172 amino acids (Mr 18,600). The C. glutamicum aspartokinase was purified and found to contain two proteins of Mr 47,000 and Mr 18,000. A C. glutamicum mutant expressing a feedback-resistant aspartokinase was shown to be changed in a single base pair of the lysC beta gene, leading to an amino acid exchange in the beta-subunit of the aspartokinase. In addition, the identified mutation was found to be responsible for the enhanced expression of the asd gene located downstream of lysC.
Kalinowski
J
J
Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, Germany.
Cremer
J
J
Bachmann
B
B
Eggeling
L
L
Sahm
H
H
Pühler
A
A
D000595:Amino Acid Sequence; D001222:Aspartate Kinase / Q000235:genetics* / Q000378:metabolism; D001412:Bacillus subtilis / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003352:Corynebacterium / Q000201:enzymology / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005246:Feedback; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005807:Genes, Overlapping; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D001222:Aspartate Kinase