Oxytetracycline inhibition of mitochondrial protein synthesis in bovine lymphocytes infected with Theileria parva or stimulated by mitogen.
Oxytetracycline (OTC) significantly inhibited cytochrome c oxidase activity in bovine lymphocytes infected with Theileria parva and in uninfected mitogen-stimulated lymphocytes. The inhibitory effect was detected in vitro within 24 h of treatment with drug concentrations as low as 1 micrograms/ml. Following mitogen stimulation of lymphocytes, concentrations of 3 and 10 micrograms/ml OTC completely inhibited an increase in cytochrome c oxidase activity for 48-72 h. This inhibitory activity was considered to be due to a direct effect on lymphoblast mitochondrial protein synthesis. As a consequence, adenosine triphosphate activity was significantly reduced in lymphocytes stimulated either by infection with T. parva sporozoites or by mitogen and then treated with OTC. The results also indicated that parasite mitochondrial protein synthesis was inhibited by OTC. The activity of OTC reported in this study could explain the suppression of disease following 'infection and treatment' immunization against East Coast fever and the in vitro drug-inhibition of schizont development.
Spooner
P R
PR
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D016782:Apicomplexa / Q000502:physiology*; D002417:Cattle; D002460:Cell Line; D002999:Clone Cells; D003208:Concanavalin A / Q000494:pharmacology; D003576:Electron Transport Complex IV / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000469:parasitology*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010118:Oxytetracycline / Q000494:pharmacology*; D014176:Protein Biosynthesis; D013801:Theileriasis / Q000469:parasitology
D003208:Concanavalin A; D000255:Adenosine Triphosphate; D003576:Electron Transport Complex IV; D010118:Oxytetracycline