title: AgNOR area in interphase nuclei of human tumours correlates with the proliferative activity evaluated by bromodeoxyuridine labelling and Ki-67 immunostaining.
abstract: The area of silver-stained proteins associated with interphase nucleolar organizer regions (AgNORs) was compared with labelling data obtained by bromodeoxyuridine (BrdU) incorporation and Ki-67 immunostaining in 25 tumours of different origins and two non-neoplastic lesions of the thyroid. Our data demonstrate a highly significant correlation between the mean area occupied by the AgNOR proteins measured by an image processing system and the proliferative indices evaluated by BrdU labelling (r = 0.89, P less than 0.001) and Ki-67 immunostaining (r = 0.86, P less than 0.001). AgNOR protein area measurement is therefore proposed as a simple, inexpensive, and reliable method of evaluating the proliferative activity in routinely processed tumour samples.
authors.affiliation: Dipartimento di Patologia Sperimentale, Università di Bologna, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Trerè
authors.forename: F
authors.initials: F
authors.lastname: Farabegoli
authors.forename: A
authors.initials: A
authors.lastname: Cancellieri
authors.forename: C
authors.initials: C
authors.lastname: Ceccarelli
authors.forename: V
authors.initials: V
authors.lastname: Eusebi
authors.forename: M
authors.initials: M
authors.lastname: Derenzini
mesh_terms: D001943:Breast Neoplasms / Q000648:ultrastructure; D001973:Bromodeoxyuridine / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000648:ultrastructure; D006044:Goiter, Nodular / Q000473:pathology; D006801:Humans; D007399:Interphase; D008113:Liver Neoplasms / Q000648:ultrastructure; D008938:Mitosis; D009369:Neoplasms / Q000648:ultrastructure*; D009687:Nuclear Proteins / Q000032:analysis; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D013964:Thyroid Neoplasms / Q000648:ultrastructure
chemical_list: D009687:Nuclear Proteins; D001973:Bromodeoxyuridine