Presence of vesicles containing lactate dehydrogenase in the dentin of bovine tooth germs.
The dentin was removed from bovine tooth germs, followed by the separation of the extracellular matrix vesicle fraction after collagenase treatment. Lactate dehydrogenase (LDH)-containing vesicles with a density different from that of matrix vesicles were detected in the matrix vesicle fraction. LDH in these vesicles did not result from cell lysis and vesicle capture during the preparation of the matrix vesicle fraction. The isoenzyme pattern of LDH in LDH-containing vesicles was similar to that of cytosolic LDH of odontoblasts. Other cytosolic enzymes were not detected in LDH-containing vesicles, suggesting the presence of a mechanism for specific uptake of cytosolic LDH during the in vivo formation of the vesicles.
Kitamura
C
C
Department of Operative Dentistry, Kyushu Dental College, Japan.
Terashita
M
M
Noguchi
T
T
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D003600:Cytosol / Q000201:enzymology; D003804:Dentin / Q000166:cytology / Q000201:enzymology*; D005109:Extracellular Matrix / Q000201:enzymology*; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D017830:Octoxynol; D009804:Odontoblasts / Q000201:enzymology; D011092:Polyethylene Glycols; D014083:Tooth Germ / Q000201:enzymology
D011092:Polyethylene Glycols; D017830:Octoxynol; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase