title: [The effect of Legionella pneumophila metalloproteinase on certain human blood proteins. Cleavage of anti(1)-antitrypsin and acid-stable serine proteinase inhibitors].
abstract: Antitryptic activity of human blood serum was decreased after incubation with metalloproteinase from Legionella pneumophila. The enzymatic activity depends on the time of incubation as well as on the ratio between the enzyme content and blood serum total protein. Cross immunoelectrophoresis, involving monospecific rabbit antiserum towards the alpha 1-antitrypsin, demonstrated highly effective hydrolysis of alpha 1-antitrypsin by the metalloproteinase. As shown by polyacrylamide gel electrophoresis the metalloproteinase hydrolyzed acid stable inhibitor of serine proteinases into fragments with distinct loss of the inhibitor activity. Thermolysine hydrolyzed similarly the proteins studied but at a lower rate. The metalloproteinase from L. pneumophila appears to be mainly responsible for production of utilizable components from protein substrates involved in vital activity of the bacteria. It may not be excluded that the enzyme is able to impair some host protective mechanisms.
authors.forename: S V
authors.initials: SV
authors.lastname: Gul'nik
authors.forename: G
authors.initials: G
authors.lastname: Feokharus
authors.forename: N V
authors.initials: NV
authors.lastname: Varchenko
authors.forename: G I
authors.initials: GI
authors.lastname: Lavrenova
authors.forename: V M
authors.initials: VM
authors.lastname: Stepanov
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007123:Immunoelectrophoresis, Two-Dimensional; D016952:Legionella pneumophila / Q000201:enzymology*; D008666:Metalloendopeptidases / Q000494:pharmacology*; D015842:Serine Proteinase Inhibitors / Q000097:blood*; D013820:Thermolysin / Q000494:pharmacology; D000515:alpha 1-Antitrypsin / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D015842:Serine Proteinase Inhibitors; D000515:alpha 1-Antitrypsin; D008666:Metalloendopeptidases; D013820:Thermolysin
vernacular_title: Deĭstvie metalloproteinazy Legionella pneumophila na nekotorye belki syvorotki krovi cheloveka. Rasshcheplenie anti1-antitripsina i kislotostabil'nogo ingibitora serinovykh proteinaz.