title: Growth hormone secretion in the guinea-pig.
abstract: The guinea-pig is unusual in that it continues to grow at a normal rate after hypophysectomy. Although its pituitary gland appears to contain a GH, this has not been isolated or characterized, and nothing is known about its secretion or physiological control. We have identified guinea-pig GH, established a sensitive heterologous radioimmunoassay and adapted our automatic blood microsampling method to study spontaneous GH secretion in this species. In male guinea-pigs, GH is released in an episodic pattern, reminiscent of the rat. Large multicomponent pulses of GH secretion occur every 3-4 h between periods of low or undetectable GH release, whereas most females showed a more uniform pulsatile pattern with pulses every 1-2 h. GH was released in response to GH-releasing factor (GRF) injections (2, 10 or 20 micrograms [Nle27]-GRF(1-29)NH2) in a dose-dependent fashion, and i.v. infusion of somatostatin (50 micrograms/h) blocked spontaneous GH pulses, eliciting a rebound release (from 2.0 +/- 0.8 (S.E.M.) to 36 +/- 17 micrograms/l 30 min after stopping the infusion). Infusions of a GH-releasing hexapeptide (100 or 400 micrograms/h for 4 h) also released GH. These results provide the first description of the pattern of GH release in the guinea-pig, and suggest that the striking episodic pattern is controlled by the same hypothalamic peptides that regulate GH in other species. Since the guinea-pig grows well in the absence of GH, this species may use GH for its metabolic, rather than growth-promoting actions. The guinea-pig may well prove a useful model, now that methods are available for studying its endogenous GH secretion.
authors.affiliation: Division of Neurophysiology and Neuropharmacology, National Institute for Medical Research, Mill Hill, London.
authors.forename: B
authors.initials: B
authors.lastname: Gabrielsson
authors.forename: K M
authors.initials: KM
authors.lastname: Fairhall
authors.forename: I C
authors.initials: IC
authors.lastname: Robinson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D013006:Growth Hormone / Q000302:isolation & purification / Q000378:metabolism*; D013007:Growth Hormone-Releasing Hormone / Q000031:analogs & derivatives / Q000494:pharmacology; D006168:Guinea Pigs; D008297:Male; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D011863:Radioimmunoassay; D012636:Secretory Rate / Q000187:drug effects; D017337:Sermorelin; D013004:Somatostatin / Q000494:pharmacology
chemical_list: D009842:Oligopeptides; D010446:Peptide Fragments; C041048:growth hormone releasing hexapeptide; D013004:Somatostatin; D017337:Sermorelin; D013006:Growth Hormone; D013007:Growth Hormone-Releasing Hormone