[Clinical significance of nuclear DNA content in human gastric tumors. A static cytometry study in 87 cases].
In recent years the abnormal DNA content of cancer cells and the ploidy model have played an important role in biological and medical research aimed at achieving a more accurate diagnostic and prognostic assessment. Static cytometry was studied in histological sections using an image analyser which enabled the DNA content of cancer cells coloured using Feulgen's method, the elective method for nucleic acids, to be evaluated. A high percentage of aneuploidy in human gastric tumours was found to be correlated with a low degree of histological differentiation (G3), with tumoral invasion of the lowest tissue strata and neighbouring lymph nodes, and with a negative prognosis. The practical clinical application of DNA evaluation therefore allows cases which evolve more rapidly since they possess a greater tumoral aggressiveness to be identified (aneuploid tumours).
Matturri
L
L
Istituto di Anatomia Patologica, Università di Milano.
Biondo
B
B
Lavezzi
A M
AM
D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D000328:Adult; D000368:Aged; D000782:Aneuploidy; D003592:Cytophotometry; D004273:DNA, Neoplasm / Q000032:analysis*; D004171:Diploidy; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology
D004273:DNA, Neoplasm
Significato clinico del contenuto nucleare in DNA nei tumori gastrici umani. Studio di citometria statica su 87 casi.