A single-column chromatographic system for the analysis and preparation of high mobility group proteins 1 and 2 and other chromosomal proteins using nondenaturing solvents.
One-step chromatography on a Mono S column allows the purification of high mobility group (HMG) proteins 1 and 2 under nondenaturing conditions. Chromatography of HMG1 and -2 on Mono S can be achieved with three of the most widely employed extraction techniques for chromosomal proteins, 0.35 M sodium chloride, 0.74 M perchloric acid, and 0.4 N sulfuric acid. In each case HMG1 and -2 are purified away from the other chromosomal proteins, histone H1, and core histones, and are resolved into both their reduced and oxidized forms. Additionally histone H1 and the core histones are fractionated on Mono S, thus the entire complement of chromosomal proteins can be analyzed in a single rapid chromatographic step.
Rice
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Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Cole
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D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006609:High Mobility Group Proteins / Q000302:isolation & purification*; D006657:Histones / Q000302:isolation & purification; D008297:Male; D009687:Nuclear Proteins / Q000302:isolation & purification*; D010472:Perchlorates; D012965:Sodium Chloride; D013464:Sulfuric Acids; D013950:Thymus Gland / Q000737:chemistry
D006609:High Mobility Group Proteins; D006657:Histones; D009687:Nuclear Proteins; D010472:Perchlorates; D013464:Sulfuric Acids; D012965:Sodium Chloride; C033158:sulfuric acid