title: Functional analysis in yeast of cDNA coding for the mitochondrial Rieske iron-sulfur protein of higher plants.
abstract: cDNA clones coding for the nuclear-encoded mitochondrial Rieske iron-sulfur protein (RISP) have been isolated from maize and tobacco. Complementation analysis of hybrid proteins consisting of different domains of plant and yeast RISPs showed that the carboxyl two-thirds of the plant protein is functionally equivalent to that of the yeast protein. The amino terminus of the RISP, however, seems to be species specific because this region is not interchangeable between plant and yeast proteins. Complementation analysis of hybrid proteins also identified a structurally conserved domain probably essential for the function of bc1 complex RISPs. A specific domain from the plant RISP was found to cause temperature-sensitive respiratory growth in yeast. We have demonstrated that yeast can serve as a model system to study the structural and functional relationships of plant gene products that are enzymatic components of the mitochondrial respiratory chain.
authors.affiliation: Department of Genetics, North Carolina State University, Raleigh 27695-7614.
authors.forename: J T
authors.initials: JT
authors.lastname: Huang
authors.forename: F
authors.initials: F
authors.lastname: Struck
authors.forename: D F
authors.initials: DF
authors.lastname: Matzinger
authors.forename: C S
authors.initials: CS
authors.lastname: Levings
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004272:DNA, Mitochondrial / Q000235:genetics*; D014450:Electron Transport Complex III; D015723:Gene Library; D005816:Genetic Complementation Test; D007506:Iron-Sulfur Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009491:Neurospora / Q000235:genetics; D010947:Plants, Toxic; D010957:Plasmids; D016354:Rhodobacter capsulatus / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D007506:Iron-Sulfur Proteins; C037989:Rieske iron-sulfur protein; D014450:Electron Transport Complex III