Novel screening method for agents that overcome classical multidrug resistance in a human cell line.
P-glycoprotein (P-gp) is involved in the transport of a wide variety of organic compounds including a fluorescent dye, rhodamine 6G (RG), as well as anti-cancer drugs. Agents that overcome classical multidrug-resistance (MDR) increased the accumulation of RG in a MDR cell line, KB-C1. The effect of agents on RG-accumulation in KB-C1 cells was highly correlated with their effect on the reversion of vincristine-resistance in KB-C1 cells. RG was detected on a fluorescence microplate reader with a rhodamine channel. This assay may become a useful method for the screening of agents that overcome classical MBR, since it is quick and simple.
Yoshimura
A
A
Cancer Research Institute, Faculty of Medicine, Kagoshima University, Japan.
Shudo
N
N
Ikeda
S I
SI
Ichikawa
M
M
Sumizawa
T
T
Akiyama
S I
SI
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004396:Coloring Agents; D004351:Drug Resistance; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007624:KB Cells / Q000187:drug effects; D008562:Membrane Glycoproteins / Q000502:physiology; D012235:Rhodamines / Q000493:pharmacokinetics; D014700:Verapamil / Q000494:pharmacology; D014750:Vincristine / Q000494:pharmacology; D014966:Xanthenes
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004396:Coloring Agents; D008562:Membrane Glycoproteins; D012235:Rhodamines; D014966:Xanthenes; C026188:rhodamine 6G; D014750:Vincristine; D014700:Verapamil