title: Use of the Tn903 neomycin-resistance gene for promoter analysis in the fission yeast Schizosaccharomyces pombe.
abstract: The bacterial neo gene from transposon Tn903 (Tn601) was used for dominant transformation of the fission yeast Schizosaccharomyces pombe. It was found that high transformation efficiency was dependent on a high level of promoter activity, mediated by the strong promoter of the Schizosaccharomyces pombe alcohol dehydrogenase gene (adh1), as shown by comparing the efficiency of transformation to G418-resistance, the resistance levels of transformed cells, and the in vitro amino-glycoside phosphotransferase activity. On the other hand, the heterologous promoter of the Saccharomyces cerevisiae alcohol dehydrogenase I gene (adc1) is shown to be a weak promoter in Schizosaccharomyces pombe, though its activity is significantly enhanced in cells grown on glycerol as a carbon source. This system for selection and detection of promoter-active sequences may provide a useful basis for the analysis of promoter elements in fission yeast.
authors.affiliation: Institut für Gärungsgewerbe und Biotechnologie, Berlin, Federal Republic of Germany.
authors.forename: C
authors.initials: C
authors.lastname: Lang-Hinrichs
authors.forename: C
authors.initials: C
authors.lastname: Dössereck
authors.forename: I
authors.initials: I
authors.lastname: Fath
authors.forename: U
authors.initials: U
authors.lastname: Stahl
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics / Q000378:metabolism; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005798:Genes, Bacterial; D019868:Kanamycin Kinase; D009355:Neomycin / Q000494:pharmacology*; D010770:Phosphotransferases / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D004251:DNA Transposable Elements; D000426:Alcohol Dehydrogenase; D010770:Phosphotransferases; D019868:Kanamycin Kinase; D009355:Neomycin