title: Expression of terminal deoxynucleotidyltransferase gene in a case of adult T-cell leukemia.
abstract: A 37-year-old male from Kagoshima Prefecture was admitted with adult T-cell leukemia (ATL). Monoclonal integration of HTLV-1 proviral DNA was found, but the integration site was different from that of terminal deoxynucleotidyltransferase (TdT)-positive MT-1 cells (an ATL cell line). The ATL cells expressed enzymatically active TdT and exhibited 2100 b TdT mRNA, which corresponds to the thymus type of TdT mRNA. The same size of TdT mRNA was also detected in MT-1. Southern blot analyses revealed no differences in the gene structure of the promoter region of TdT genes between this ATL case and TdT-positive lymphoblastic leukemia cells. There is little possibility that cis-acting viral elements promote TdT gene expression by proviral integration. The activation of TdT gene in ATL may be mediated by other trans-acting factors.
authors.affiliation: Department of Pediatrics, Nagoya University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Oiwa
authors.forename: K
authors.initials: K
authors.lastname: Horibe
authors.forename: O
authors.initials: O
authors.lastname: Koiwai
authors.forename: T
authors.initials: T
authors.lastname: Kaneda
authors.forename: Y
authors.initials: Y
authors.lastname: Nakaide
authors.forename: S
authors.initials: S
authors.lastname: Tamori
authors.forename: T
authors.initials: T
authors.lastname: Kodaka
authors.forename: T
authors.initials: T
authors.lastname: Uchiyama
mesh_terms: D000328:Adult; D004253:DNA Nucleotidylexotransferase / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D015458:Leukemia, T-Cell / Q000235:genetics*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D004253:DNA Nucleotidylexotransferase