The influence of LH and/or FSH on Leydig and Sertoli cell morphology after testicular involution in the Djungarian hamster, Phodopus sungorus, induced by hypophysectomy or short photoperiods.
In the Djungarian hamster, Phodopus sungorus, the morphological alterations of Sertoli and Leydig cells were investigated under the influence of gonadotropins (LH and/or FSH) after testicular regression induced either by hypophysectomy or photoinhibition. Stimulation with LH or LH/FSH lead to a redifferentiation of morphological features of Leydig cells such as nuclear structure, smooth endoplasmic reticulum and size of cell and nucleus area within 7 days of hormone treatment. Similarly, stimulation with FSH or LH/FSH caused redifferentiation of Sertoli cell nuclear structure, rough endoplasmic reticulum and nuclear size within 7 days. Incomplete restoration of Leydig and Sertoli cell morphology was observed under FSH and LH treatment respectively. In both Leydig and Sertoli cells combined LH and FSH application resulted in an increased response in respect to morphological redifferentiation, possibly indicating paracrine regulatory mechanisms. In all groups treated an intact blood-testis barrier (BTB) was reestablished after a minimum of 7 days, indicating that the existence of the blood-testis barrier is not dependent on specific gonadotropin supply but on the developmental stage of the seminiferous epithelium. Sham-operated animals showed increased cell and nucleus area of Leydig cells in comparison to photostimulated animals. After testicular involution as well as after LH or FSH treatment there were no significant morphological differences between hypophysectomized and photoinhibited animals in respect to the documented ultrastructural and morphometrical characteristics.(ABSTRACT TRUNCATED AT 250 WORDS)
Kliesch
S
S
Department of Anatomy, University of Münster, Germany.
Schweifer
B
B
Niklowitz
P
P
Nieschlag
E
E
Bergmann
M
M
D000818:Animals; D001814:Blood-Testis Barrier / Q000502:physiology; D002467:Cell Nucleus / Q000648:ultrastructure; D006224:Cricetinae; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D007016:Hypophysectomy; D007985:Leydig Cells / Q000187:drug effects / Q000648:ultrastructure*; D008027:Light; D007986:Luteinizing Hormone / Q000494:pharmacology*; D008297:Male; D008854:Microscopy, Electron; D010507:Periodicity; D012098:Reproduction / Q000502:physiology; D012708:Sertoli Cells / Q000187:drug effects / Q000648:ultrastructure*; D013737:Testis / Q000502:physiology*
D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone