Analysis of ultrastructural immune deposits in passive heymann nephritis by double-staining methods with electron microscopy.
In this study, we located and quantitated the ultrastructural glomerulus-bound immunoglobulins of passive Heymann nephritis from 10 min through 64 days using the double immunogold staining method. At the early stage (10 min to 21 days), rabbit IgG was found predominantly comparing rat IgG. With time (from 21 days to 32 days), rat IgG increased, although rabbit IgG was almost the same as at the early stage. At the late stage (64 days), rat IgG was predominantly found in all of the dense deposits on the glomerular basement membrane, whereas rabbit IgG was decreased. Thus, the double staining method was useful to analyze the composition of the immune complex and also to elucidate the immunopathogenesis of immune complex-mediated glomerulonephritis.
Kitazawa
K
K
Department of Nephrology, Showa University School of Medicine, Tokyo, Japan.
Shioda
S
S
Nakai
Y
Y
Tsumuraya
T
T
Sugisaki
T
T
D000818:Animals; D001485:Basement Membrane / Q000276:immunology / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D005921:Glomerulonephritis / Q000276:immunology / Q000473:pathology* / Q000503:physiopathology; D007074:Immunoglobulin G / Q000032:analysis*; D007136:Immunoglobulins / Q000032:analysis*; D007678:Kidney Glomerulus / Q000276:immunology / Q000648:ultrastructure*; D008297:Male; D016253:Microscopy, Immunoelectron; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D007074:Immunoglobulin G; D007136:Immunoglobulins