The role of ascorbate in the biosynthesis of neuroendocrine peptides.
The biosynthesis of all alpha-amidated peptides requires the participation of peptidylglycine alpha-amidating monooxygenase (PAM), a bifunctional enzyme dependent on molecular oxygen, ascorbate, and copper. In the AtT-20 corticotrope cell line, reduced concentrations of PAM and ascorbate can make alpha-amidation the rate-limiting step in the production of bioactive product peptide. Production of various peptides thought to play autocrine and paracrine roles may be affected by ascorbate availability.
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Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
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D000818:Animals; D001205:Ascorbic Acid / Q000502:physiology*; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D009097:Multienzyme Complexes; D009490:Neurosecretory Systems / Q000378:metabolism*; D010452:Peptide Biosynthesis; D010455:Peptides / Q000737:chemistry; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics
D009097:Multienzyme Complexes; D010455:Peptides; D012333:RNA, Messenger; D004247:DNA; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase; D001205:Ascorbic Acid