title: Enzyme immunoassay of somatostatin (SS)-like immunoreactive substance in bovine milk.
abstract: A sensitive and specific enzyme immunoassay (EIA) for somatostatin (SS)-like immunoreactivity (SS-LI) was developed with the use of beta-D-galactosidase labeled antigen. The minimum amount of SS-like immunoreactive substance (SS-IS) detectable by this method was 1.0 fmol/well (25 pmol/l). The level of SS-IS in bovine foremilk was about 20 pmol/l, and the level was unchanged after delivery. On the other hand, the levels of gastrin releasing peptide (GRP)-IS and vasoactive intestinal polypeptide (VIP)-IS in bovine foremilk were very high, but fell during 1 week after delivery to about 10% of those in foremilk.
authors.affiliation: Department of Hospital Pharmacy, Medical College of Oita, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Takeyama
authors.forename: N
authors.initials: N
authors.lastname: Yanaga
authors.forename: K
authors.initials: K
authors.lastname: Yarimizu
authors.forename: J
authors.initials: J
authors.lastname: Ono
authors.forename: R
authors.initials: R
authors.lastname: Takaki
authors.forename: N
authors.initials: N
authors.lastname: Fujii
authors.forename: H
authors.initials: H
authors.lastname: Yajima
mesh_terms: D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D007124:Immunoenzyme Techniques; D008892:Milk / Q000032:analysis*; D013004:Somatostatin / Q000032:analysis*
chemical_list: D013004:Somatostatin