[An analysis of gp 70 expressed on Friend virus-infected rat tumor cells and the mouse melanoma antigen cross-reacting with it].
In order to analyse a virus-associated antigen which is expressed on Friend virus-infected rat tumor cells and induces strong resistance to their transplantability. I made monoclonal antibodies against it by using spleen cells of the syngeneic rats in whom the Friend virus-associated tumor cells spontaneously regressed. As a result, I was able to obtain a monoclonal antibody, named TF 1, to react specifically to the infection of Friend virus and found out that TF1 antibody reacted to gp 70, a product of the envelope gene of Friend virus, on Friend virus-infected rat tumor cells. And also I found out that B16BL6 cells, which are mouse melanoma cells and are not infected with Friend virus, were positive to TF1 antibody and made it clear that the melanoma antigen which reacted to TF 1 antibody was not gp 70, but a protein of 80-85 KD. Immunizing therefore with gp 70 cross-reacting with the melanoma antigen, I was able to observe that resistance to the transplantability of B16BL6 cells was induced. The result suggests that the melanoma antigen played a role as a tumor rejection antigen. I thus consider that gp 70 is able to induce resistance to transplantability of B16BL6 cells in mice and affects spontaneous regression of Friend virus-infected tumor cells as a virus-associated antigen in rats.
Furune
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Laboratory of Pathology, Hokkaido University School of Medicine, Sapporo, Japan.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D005622:Friend murine leukemia virus / Q000235:genetics / Q000276:immunology*; D006084:Graft Rejection; D008546:Melanoma, Experimental / Q000276:immunology*; D051379:Mice; D009368:Neoplasm Transplantation; D009856:Oncogene Proteins, Viral / Q000032:analysis / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D009856:Oncogene Proteins, Viral; C055694:env-sea oncogene protein, Avian Erythroblastosis Retrovirus