title: Evaluation of combined commercial enzyme-linked immunosorbent assay for detection of rota and adenoviruses for automation.
abstract: 219 stools were examined by direct electron microscopy (EM), culture and 'combined' commercial enzyme-linked immunosorbent assay kits (CELISA). The specificity of the combined ELISA for rotavirus was 100% as compared with EM, and 100% for adenovirus when both culture in addition to EM were carried out. ELISA appeared to be more sensitive than EM for both viruses. There was no cross-reaction between the 2 'combined' antisera. This technique may be useful for automation of viral diagnosis with ELISA using a 'panel' of selected viruses for a variety of specimens.
authors.affiliation: Department of Laboratory Medicine, Royal Alexandra Hospital, Edmonton, Alberta, Canada.
authors.forename: E Y
authors.initials: EY
authors.lastname: Cheung
authors.forename: S I
authors.initials: SI
authors.lastname: Hnatko
authors.forename: H
authors.initials: H
authors.lastname: Gunning
authors.forename: R
authors.initials: R
authors.lastname: Thomas
mesh_terms: D000256:Adenoviridae / Q000302:isolation & purification* / Q000648:ultrastructure; D000818:Animals; D001322:Autoanalysis; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology*; D005260:Female; D008297:Male; D012401:Rotavirus / Q000302:isolation & purification* / Q000648:ultrastructure; D014709:Vero Cells / Q000382:microbiology