MHV-resistant SJL/J mice express a non-functional homolog to the MHV receptor glycoprotein.
MHV-A59 recognizes a 100-110 K glycoprotein receptor on intestinal and liver plasma membranes. The receptor protein was affinity purified using an anti-receptor monoclonal antibody. Purified receptor was sequenced from the 1st to the 15th amino acids. Antiserum raised against a synthetic peptide having this sequence bound to the 110 K receptor from BALB/c intestine and a 58 K fragment of the receptor. SJL/J mice are resistant to MHV-A59 and their plasma membranes were previously shown to lack binding to MHV. However, anti-peptide antibody bound to a 105 K protein and a 53 K protein fragment from SJL/J plasma membranes. These SJL/J proteins did not bind MHV or to the blocking monoclonal anti-receptor antibody. Genetic resistance of SJL/J mice to MHV-A59 appears to be due to mutation of the receptor glycoprotein resulting in a molecule that does not bind MHV.
Williams
R K
RK
Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814.
Snyder
S W
SW
Holmes
K V
KV
D000818:Animals; D000911:Antibodies, Monoclonal; D002462:Cell Membrane / Q000502:physiology*; D004198:Disease Susceptibility / Q000276:immunology; D020022:Genetic Predisposition to Disease; D006524:Hepatitis, Viral, Animal / Q000235:genetics / Q000276:immunology*; D007113:Immunity, Innate / Q000235:genetics; D015151:Immunoblotting; D007422:Intestines / Q000382:microbiology / Q000502:physiology; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D008871:Microvilli / Q000502:physiology; D008970:Molecular Weight; D006517:Murine hepatitis virus / Q000276:immunology*; D011991:Receptors, Virus / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology
D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D011991:Receptors, Virus