Fluorocarbon/lecithin emulsions: identification of EYP-coated fluorocarbon droplets and fluorocarbon-empty vesicles by freeze-fracture electron microscopy.
Freeze-fracture has been used to examine perfluorodecalin/egg yolk phospholipid emulsions (70:8, w/v%) destined to be used as injectable oxygen carriers. The fluorocarbon displays a specific granular aspect upon freeze-fracture which makes it readily recognizable and allows the distinction between two populations of objects on the micrographs: fluorocarbon droplets and water-filled lipid vesicles.
Postel
M
M
Laboratoire de Chimie Moléculaire, U.R.A. CNRS, Université de Nice-Sophia Antipolis, France.
Chang
P
P
Rolland
J P
JP
Krafft
M P
MP
Riess
J G
JG
D004530:Egg Yolk; D004655:Emulsions; D005466:Fluorocarbons / Q000737:chemistry*; D005614:Freeze Fracturing; D008854:Microscopy, Electron; D010743:Phospholipids / Q000737:chemistry
D004655:Emulsions; D005466:Fluorocarbons; D010743:Phospholipids; C008805:perfluorodecalin