title: Analysis and characterization of the interleukin 2 receptor alpha and beta chain expression in CD4-CD8- cells.
abstract: The differential effects that the binding of interleukin 2 (IL-2) to its beta or alpha beta receptors might induce in two different CD4-CD8- T-cell lines were analysed. While LD1.T3b, a double-negative T cell derived from MRL/lpr mice, constitutively expressed high levels of the IL-2R beta chain, YAC-1, a Moloney sarcoma virus-transformed CD4-CD8- T cell, expressed (as an activated T cell) the beta and alpha chains. The presence of IL-2 in the culture medium was lethal for LD1.T3b cells, while it had no effect on the growth of YAC-1 cells. IL-2 increased the expression of the beta chain and, to a lesser extent, of the alpha chain in YAC-1 cells. In addition, other markers such as CD4 and CD5 were induced by IL-2 in this cell line.
authors.affiliation: Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma de Madrid, Spain.
authors.forename: J C
authors.initials: JC
authors.lastname: Gutiérrez-Ramos
authors.forename: L
authors.initials: L
authors.lastname: Pezzi
authors.forename: E
authors.initials: E
authors.lastname: Leonardo
authors.forename: C
authors.initials: C
authors.lastname: Martin
authors.forename: C
authors.initials: C
authors.lastname: Martínez
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000032:analysis* / Q000276:immunology; D002460:Cell Line; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D010641:Phenotype; D015375:Receptors, Interleukin-2 / Q000032:analysis* / Q000235:genetics; D050378:T-Lymphocytes, Regulatory / Q000032:analysis* / Q000276:immunology
chemical_list: D007376:Interleukin-2; D015375:Receptors, Interleukin-2