The effect of post-irradiation anisotonic treatment on cell survival and repair of DNA damage.
V79 379A cells were irradiated and then exposed to anisotonic PBS for 20 min. This enhanced the radiation effect resulting from the fixation of potentially lethal damage. The induction of DNA single- and double-strand breaks is not increased by this treatment. Anisotonic treatment delayed the onset of repair of DNA damage. However when cells were returned to normal medium, they repaired the damage to a similar extent as cells not exposed to the anisotonic treatment. We suggest that the fixation of damage by post-irradiation anisotonic treatment is mediated through an increased probability of misrepair of DNA damage due to the delay in the onset of repair. This is supported by the observation that there is a reduced effect of post-irradiation anisotonic treatment on cells that have a markedly reduced ability to repair double-strand breaks.
McNally
N J
NJ
Gray Laboratory of the Cancer Research Campaign, Mount Vernon Hospital, Northwood, Middlesex, UK.
Hinchliffe
M
M
Soranson
J
J
D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects*; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D004247:DNA / Q000032:analysis; D004260:DNA Repair / Q000187:drug effects*; D006982:Hypertonic Solutions; D011829:Radiation Dosage; D012965:Sodium Chloride / Q000494:pharmacology*
D003470:Culture Media; D006982:Hypertonic Solutions; D012965:Sodium Chloride; D004247:DNA