Molecular analysis of periodontal pathogens.
Both Bacteroides gingivalis and Actinobacillus actinomycetemcomitans have been implicated in the destruction of periodontal tissues. To understand better the role putative virulence factors from the two bacterial species may play in an infection, the collagenase gene from Bact. gingivalis and the leucotoxin gene from A. actinomycetemcomitans were cloned. As it is intended to generate strains carrying defined mutations in these genes for in vitro and in vivo experiments, extensive restriction mapping and sequence analyses of these clones are being undertaken. Furthermore a conjugation system for Bact. gingivalis and A. actinomycetemcomitans will be established.
Lawson
D A
DA
Max-Planck-Institut für Biologie, Abteilung Infektionsbiologie, Tübingen, West Germany.
Haas
R
R
Meyle
J
J
Meyer
T F
TF
D000188:Actinobacillus / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D001427:Bacterial Toxins / Q000235:genetics; D001439:Bacteroides / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D003001:Cloning, Molecular; D003603:Cytotoxins / Q000235:genetics; D005098:Exotoxins / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D015698:Genomic Library; D006801:Humans; D003012:Microbial Collagenase / Q000378:metabolism; D009876:Operon / Q000235:genetics; D010518:Periodontitis / Q000382:microbiology*; D014774:Virulence
D001427:Bacterial Toxins; D003603:Cytotoxins; D005098:Exotoxins; C018579:leukotoxin; D003012:Microbial Collagenase