Direct evidence that the FimH protein is the mannose-specific adhesin of Escherichia coli type 1 fimbriae.
Type 1 fimbriae of Escherichia coli are surface organelles which mediate binding to D-mannose-containing structures. By direct binding of FimH to D-mannose attached to a carrier protein, we demonstrated that this protein was uniquely responsible for the receptor specificity. Furthermore, we show by receptor immunoelectron microscopy that the FimH protein is located laterally in the structure of the type 1 fimbriae.
Krogfelt
K A
KA
Department of Microbiology, Technical University of Denmark, Lyngby.
Bergmans
H
H
Klemm
P
P
D018830:Adhesins, Escherichia coli; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D005798:Genes, Bacterial; D008358:Mannose / Q000378:metabolism*; D008854:Microscopy, Electron; D008970:Molecular Weight; D010957:Plasmids; D011994:Recombinant Proteins
D018830:Adhesins, Escherichia coli; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D011994:Recombinant Proteins; C058769:fimH protein, E coli; D036781:Fimbriae Proteins; D008358:Mannose