title: Correlation between DNA quantities in agarose gel and chromatographic areas of photographed DNA bands.
abstract: To quantitate DNA from photographs, correlations between chromato-scanned (in the fluorescence mode) and virtual areas from white DNA bands on photographs were examined. A linear correlation between the two measurements with coefficients higher than 0.89 was observed. The dynamic range of the fluorescence detection yielded good linearity for a wide range of amounts of DNA (microgram-ng) on the photograph.
authors.affiliation: Department of Virology, Kurume University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Chinami
authors.forename: E
authors.initials: E
authors.lastname: Tanikawa
authors.forename: H
authors.initials: H
authors.lastname: Hachisuka
authors.forename: Y
authors.initials: Y
authors.lastname: Sasai
authors.forename: M
authors.initials: M
authors.lastname: Shingu
mesh_terms: D002845:Chromatography; D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D010781:Photography
chemical_list: D004247:DNA