Low tumorigenicity of canine cells transformed by the human cytomegalovirus.
Dog embryo kidney cells transformed by the human cytomegalovirus (HCMV) were obtained after non-permissive infection or transfection with viral DNA digested by restriction endonuclease EcoR I. The transformed cells, growing rapidly and showing an unlimited division potential, could use medium with only 2% serum for growth, contained nuclear virus antigens, and formed small colonies (less than 0.2 mm) in agarose. From 40 mice inoculated with transformed canine cells, only one eventually developed a tumor. Results indicate that dog cells are immortalized but not tumorigenically transformed by the human cytomegalovirus.
Yelle
J
J
Centre de recherche en virologie, Institut Armand-Frappier, Laval-des-Rapides, Québec, Canada.
Lussier
G
G
Pramatarova
A
A
Hamelin
C
C
D000818:Animals; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D004279:DNA, Viral / Q000235:genetics; D004285:Dogs; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000209:etiology / Q000382:microbiology / Q000473:pathology; D014162:Transfection
D004279:DNA, Viral