Wheat protein antigens and effector cells of aspecific immunity.
The effects of gliadin and glyc-gli on leukocyte chemiluminescence response, cytotoxic activity and locomotion were assessed in vitro. A dose-dependent increase in chemiluminescence response of neutrophils stimulated by Zymosan was observed by using gliadin at concentrations ranging between 1 and 20 micrograms. By increasing glyc-gli concentrations, a bimodal response was observed with an enhancement up to 50 micrograms/ml, followed by dose-dependent suppressive effects. The cytotoxic activity of a suspension of peripheral blood mononuclear cells on the human myeloid line K562 was assessed in a Chromium release assay. By pretreating effector cells with optimal doses of gliadin (5 micrograms/ml) or glyc-gli (50 micrograms/ml), an enhancement of cytotoxic activity, similar to that of the gamma-Interferon, could be achieved. Finally glyc-gli was found to elicit neutrophil chemokinesis. The possible implications of these findings in diseases characterized by gluten intolerance are discussed.
Roccatello
D
D
Cattedra di Nefrologia Medica, Banca del Sangue, di Torino, Italy.
Coppo
R
R
Cavalli
G
G
Piccoli
G
G
Amprimo
M C
MC
Guerra
M G
MG
Amore
A
A
Di Mauro
C
C
Quattrocchio
G
G
Cacace
G
G
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005903:Gliadin / Q000276:immunology / Q000494:pharmacology*; D005983:Glutens / Q000276:immunology / Q000494:pharmacology*; D006801:Humans; D007694:Killer Cells, Natural / Q000187:drug effects*; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D015054:Zymosan / Q000494:pharmacology
D000911:Antibodies, Monoclonal; C066008:glyc-gli (gluten fraction); D005983:Glutens; D005903:Gliadin; D015054:Zymosan