Sequencing and expression of aadA, bla, and tnpR from the multiresistance transposon Tn1331.
A fragment of Tn1331 including tnpR, aac, aadA, and a bla gene which encodes lower levels of resistance to ampicillin and carbenicillin as compared to those mediated by the TEM beta-lactamase was sequenced. The polypeptide encoded by the bla gene has homology with the OXA-1, PSE-2, and OXA-2 proteins. Genes aac and bla are upstream and downstream respectively of aadA, and are both flanked by recombinational hot spots. Tn1331 has 520-bp direct repeats which include parts of the tnpR and TEM bla genes. Evolutionary models for the genesis of Tn1331 are proposed.
Tolmasky
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Department of Microbiology and Immunology, School of Medicine, Oregon Health Sciences University, Portland 97201-3098.
D000595:Amino Acid Sequence; D002872:Chromosome Deletion; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011815:R Factors; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D001618:beta-Lactamases / Q000235:genetics
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D001618:beta-Lactamases