title: [Use of digoxigenin-labeled probes for the detection of papillomaviruses by in situ hybridization].
abstract: A technique of detection of human papillomaviruses by in situ hybridization in paraffin-embedded formalin-fixed sections of genito-anal lesions is described. The probes are labeled by incorporation of digoxigenin-labeled nucleotides. The hybrids are revealed by immunohistochemistry with an anti-digoxigenin antibody coupled with alkaline phosphatase. This technique is sensitive, cheap and may be used in routine in laboratories of pathology.
authors.affiliation: Laboratoire Pol Bouin, I.N.S.E.R.M. U. 314, Hôpital Maison-blanche, Reims.
authors.forename: C
authors.initials: C
authors.lastname: Clavel
authors.forename: I
authors.initials: I
authors.lastname: Binninger
authors.forename: M
authors.initials: M
authors.lastname: Polette
authors.forename: M C
authors.initials: MC
authors.lastname: Boutterin
authors.forename: P
authors.initials: P
authors.lastname: Birembaut
mesh_terms: D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D004076:Digoxigenin; D006801:Humans; D007150:Immunohistochemistry; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D004076:Digoxigenin
vernacular_title: Utilisation de sondes marquées par la digoxigenine pour la recherche des papillomavirus par hybridation in situ.