title: The DNA content of some mammalian cells measured by flow cytometry and its influence on radiation sensitivity.
abstract: The DNA content of nine mammalian cell lines was determined by flow cytometry. Using radiobiological data from this and other laboratories a correlation between DNA mass and 1/D0 for X-rays, alpha-particles, and heavy ions could be established when the quantities were plotted on a log-log scale. The slopes of the regression lines amounted to 0.65 (X-rays), 0.64 (alpha-particles) and 0.74 (heavy ions). A similar correlation was found between DNA content and mean inactivation dose. The rather uniform slopes close to 2/3 suggest that radiosensitivity may depend on the surface area of the sensitive target, (cell nucleus) indicating a possible non-uniform distribution of radiosensitive sites within the nucleus.
authors.affiliation: Institut für Genetik und für Toxikologie, Kernforschungszentrum Karlsruhe, FRG.
authors.forename: W
authors.initials: W
authors.lastname: Sontag
authors.forename: G
authors.initials: G
authors.lastname: Knedlitschek
authors.forename: K F
authors.initials: KF
authors.lastname: Weibezahn
authors.forename: H
authors.initials: H
authors.lastname: Dertinger
mesh_terms: D000512:Alpha Particles; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D006224:Cricetinae; D004247:DNA / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D007477:Ions; D051379:Mice; D011836:Radiation Tolerance
chemical_list: D004273:DNA, Neoplasm; D007477:Ions; D004247:DNA