title: The kinetics of electron entry in cytochrome c oxidase.
abstract: The kinetics of electron entry in beef heart cytochrome c oxidase have been studied by stopped-flow spectroscopy following chemical modification of the CuA site with mercurials. In this derivative CuA is no longer reducible by cytochrome c while cytochrome alpha may accept electrons from the latter with rates comparable to the native enzyme. The results indicate that CuA is not the exclusive electron entry site in cytochrome c oxidase.
authors.affiliation: Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Universitá di Roma Tor Vergata, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Malatesta
authors.forename: G
authors.initials: G
authors.lastname: Antonini
authors.forename: P
authors.initials: P
authors.lastname: Sarti
authors.forename: B
authors.initials: B
authors.lastname: Vallone
authors.forename: M
authors.initials: M
authors.lastname: Brunori
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D003300:Copper / Q000378:metabolism; D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D009206:Myocardium / Q000201:enzymology*; D010084:Oxidation-Reduction
chemical_list: D003300:Copper; D003576:Electron Transport Complex IV