A glucose-repressible gene encodes acetyl-CoA hydrolase from Saccharomyces cerevisiae.
Acetyl-CoA hydrolase, catalyzing the hydrolysis of acetyl-CoA, is presumably involved in regulating the intracellular acetyl-CoA pool. Recently, a yeast acetyl-CoA hydrolase was purified to homogeneity from Saccharomyces cerevisiae and partially characterized (Lee, F.-J. S., Lin, L.-W., and Smith, J. A. (1989) Eur. J. Biochem. 184, 21-28). In order to study the biological function and regulation of the acetyl-CoA hydrolase, we cloned and sequenced the full length cDNA encoding yeast acetyl-CoA hydrolase. RNA blot analysis indicates that acetyl-CoA hydrolase is encoded by a 2.5-kilobase mRNA. DNA blot analyses of genomic and chromosomal DNA reveal that the gene (so-called ACH1, acetyl-CoA hydrolase) is present as a single copy located on chromosome II. Acetyl-CoA hydrolase is established to be a mannose-containing glycoprotein, which binds concanavalin A. By measuring the levels of ACH1 mRNA and acetyl-CoA hydrolase activity in different growth phases and by examining the effects of various carbon sources, we have demonstrated that ACH1 expression is repressed by glucose.
Lee
F J
FJ
Department of Molecular Biology, Massachusetts General Hospital, Boston.
Lin
L W
LW
Smith
J A
JA
D000104:Acetyl-CoA Hydrolase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D015256:Coenzyme A-Transferases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D004794:Enzyme Repression; D015723:Gene Library; D005800:Genes, Fungal / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010449:Peptide Mapping; D011487:Protein Conformation; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D013869:Thiolester Hydrolases / Q000235:genetics*; D014357:Trypsin
D029701:Saccharomyces cerevisiae Proteins; C000603661:Ach1 protein, S cerevisiae; D015256:Coenzyme A-Transferases; D013869:Thiolester Hydrolases; D000104:Acetyl-CoA Hydrolase; D014357:Trypsin; D005947:Glucose