title: Probestin, a new inhibitor of aminopeptidase M, produced by Streptomyces azureus MH663-2F6. I. Taxonomy, production, isolation, physico-chemical properties and biological activities.
abstract: Probestin has been isolated as part of a program designed to find microorganism-produced inhibitors of aminopeptidase M from Streptomyces azureus MH663-2F6. It was purified by use of column chromatography of Amberlite XAD-4, silica gel, YMC-gel, Toyopearl HW-40, YMC D-ODS-5 (HPLC) and then isolated as colorless powders. Probestin is competitive with the substrate, and the inhibition constant (Ki) of it was 1.9 x 10(-8) M.
authors.affiliation: Institute of Microbial Chemistry, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Aoyagi
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Y
authors.initials: Y
authors.lastname: Shigihara
authors.forename: M
authors.initials: M
authors.lastname: Hamada
authors.forename: T
authors.initials: T
authors.lastname: Takeuchi
mesh_terms: D000626:Aminopeptidases / Q000037:antagonists & inhibitors*; D000818:Animals; D018826:CD13 Antigens; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003470:Culture Media / Q000032:analysis; D007700:Kinetics; D051379:Mice; D009842:Oligopeptides / Q000302:isolation & purification* / Q000494:pharmacology / Q000633:toxicity; D013302:Streptomyces / Q000032:analysis*
chemical_list: D003470:Culture Media; D009842:Oligopeptides; C063432:probestin; D000626:Aminopeptidases; D018826:CD13 Antigens