The isolation of high molecular weight DNA from wheat, barley and rye for analysis by pulse-field gel electrophoresis.
A method is presented for the preparation of large DNA molecules from protoplasts embedded in agarose blocks of three different cereals--hexaploid bread wheat (Triticum aestivum), barley (Hordeum vulgare) and rye (Secale cereale). Pulse-field gel electrophoresis (PFGE) analysis of these DNA preparations using a contour-clamped homogeneous field (CHEF) apparatus indicated that the size of the DNA molecules was greater than 6 Mb. DNA samples prepared by this method were shown to be useful for restriction analysis using both frequent and rare cutting enzymes.
Cheung
W Y
WY
Cambridge Laboratory, Colney Lane, Norwich, UK.
Gale
M D
MD
D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D001467:Hordeum / Q000032:analysis / Q000235:genetics; D008970:Molecular Weight; D010944:Plants / Q000032:analysis* / Q000235:genetics; D012434:Secale / Q000032:analysis / Q000235:genetics; D014908:Triticum / Q000032:analysis / Q000235:genetics
D004247:DNA; D004262:DNA Restriction Enzymes