Formation of 12-lipoxygenase metabolites in rat cerebral cortical slices: stimulation by calcium ionophore, glutamate and N-methyl-D-aspartate.
The 12-lipoxygenase pathway of arachidonic acid metabolism in rat cerebral cortex slices is stimulated by exogenous arachidonic acid, Ca2+ ionophore A23187, phorbol ester, glutamate, N-methyl-D-aspartate (NMDA) but not by kainate and other neurotransmitters except norepinephrine. The 12-hydroxyeicosatetraenoic acid formed is the (S)-enantiomer. A specific role for 12-lipoxygenase metabolites in NMDA receptor activation and long term potentiation is proposed.
Wolfe
L S
LS
Donner Laboratory of Experimental Neurochemistry, Montreal Neurological Institute, McGill University, Quebec, Canada.
Pellerin
L
L
Drapeau
C
C
Rostworowski
K
K
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D001092:Arachidonate 12-Lipoxygenase / Q000378:metabolism*; D001091:Arachidonate Lipoxygenases / Q000378:metabolism*; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology*; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism; D066298:In Vitro Techniques; D016202:N-Methylaspartate; D018377:Neurotransmitter Agents / Q000494:pharmacology; D010703:Phorbol Esters / Q000494:pharmacology*
D005971:Glutamates; D006893:Hydroxyeicosatetraenoic Acids; D018377:Neurotransmitter Agents; D010703:Phorbol Esters; D001224:Aspartic Acid; D000001:Calcimycin; D018698:Glutamic Acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D016202:N-Methylaspartate; D001091:Arachidonate Lipoxygenases; D001092:Arachidonate 12-Lipoxygenase