Combination of mutant EL-4 thymoma cells and EBV-infection in B lymphocyte activation a) EBV-infection of EL-4-activated lymphocytes. b) Use of EL-4 cells as feeders for lymphoblastoid cell lines.
The combination of mutant EL-4 thymoma cells and Epstein-Barr virus (EBV)-infection in the activation of human B lymphocytes was studied with two approaches: a) limiting numbers of B cells were first activated by EL-4 contact in order to expand the B cell population and then infected with EBV. Results show that EBV could induce further Ig synthesis, although was unable to determine proliferation or to generate immortalized lines from EL-4 activated cells. b) EL-4 cells were compared to conventional PBM as feeders for cultures of established lymphoblastoid cell lines (LCL) at low cell densities. EL-4 feeder activity was strictly dependent on the presence of phorbol-myristate-acetate (PMA) and supernatant from stimulated T-lymphocytes (T-SN). EL-4 feeders induced earlier proliferation peak and greater Ig synthesis by LCL. The latter effect could be also mediated by the addition of PMA and T-SN, even if a cooperating cell-to-cell signal by PMA and T-SN-sensitized-EL-4 cells could not be excluded. Altogether results indicate that EL-4 cells do not represent a clear advantage over classic PBM as feeders for cultures of established LCL at low cell densities.
Barcellini
W
W
Istituto di Clinica Medica I, University of Milan, Italy.
Borghi
M O
MO
Nicoletti
F
F
Bonara
P
P
Fain
C
C
Meroni
P L
PL
D001402:B-Lymphocytes / Q000276:immunology* / Q000382:microbiology; D002450:Cell Communication / Q000187:drug effects; D002455:Cell Division; D002460:Cell Line; D006566:Herpesviridae Infections / Q000503:physiopathology*; D004854:Herpesvirus 4, Human; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects; D009154:Mutation; D013234:Stem Cells / Q000166:cytology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013945:Thymoma / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000502:physiology / Q000528:radiation effects
D013755:Tetradecanoylphorbol Acetate