Recovery of murine myelopoiesis after cytostatic reduction by Ara-C. Effect of bacitracin-induced changes in the intestinal microflora and influence of timing.
The influence of intestinal flora modulation by oral bacitracin on the recovery of myelopoiesis after Ara-C was studied in C3H/Law mice. Bacitracin resulted in a 3-5 log increase of Gram-negative bacteria and a 10-fold increase of the intestinal endotoxin concentration. Initiation of bacitracin before Ara-C stimulated the initial rebound increase of colony-forming units for granulocytes and macrophages (CFU-GM) from 23.2 +/- 1.3 to 28.4 +/- 1.4 x 10(3) per femur. Starting the bacitracin after Ara-C advanced the second phase of the rebound CFU-GM increase with 6 days. An important role in the recovery of myelopoiesis after cytostatic drugs in C3H/Law mice is suggested for the intestinal Gram-negative microflora, probably mediated by bacterial endotoxin.
Daenen
S
S
Department of Haematology, University of Groningen, The Netherlands.
Goris
H
H
de Boer
F
F
Halie
M R
MR
van der Waaij
D
D
D000818:Animals; D001414:Bacitracin / Q000494:pharmacology*; D001772:Blood Cell Count; D001853:Bone Marrow / Q000187:drug effects*; D003114:Colony-Forming Units Assay; D003561:Cytarabine / Q000633:toxicity*; D004731:Endotoxins / Q000032:analysis; D006410:Hematopoiesis / Q000187:drug effects; D007422:Intestines / Q000187:drug effects / Q000382:microbiology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H
D004731:Endotoxins; D003561:Cytarabine; D001414:Bacitracin