title: GAL4 is phosphorylated as a consequence of transcriptional activation.
abstract: GAL4 protein isolated from yeast in which it is active is phosphorylated predominantly on two different serine residues. One of these was identified as Ser-837; substitution of this residue for alanine has no detectable effect on transcriptional activation by GAL4. Phosphorylation at Ser-837 requires that both the DNA binding and transcriptional activation functions be intact. We propose that some phosphorylations of GAL4, including that at Ser-837, occur concomitantly with activation of transcription.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
authors.forename: I
authors.initials: I
authors.lastname: Sadowski
authors.forename: D
authors.initials: D
authors.lastname: Niedbala
authors.forename: K
authors.initials: K
authors.lastname: Wood
authors.forename: M
authors.initials: M
authors.lastname: Ptashne
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002872:Chromosome Deletion; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D010766:Phosphorylation; D010768:Phosphoserine / Q000378:metabolism; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D012694:Serine; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D010768:Phosphoserine; D012694:Serine; D008715:Methionine