title: Pathological and immunological study of goose embryos experimentally infected with duck plague virus.
abstract: A total of 240 embryonated goose eggs obtained from two susceptible flocks were used. Half of the eggs were inoculated into the allantoic cavity with a virulent strain (7593) of duck plague virus isolated from an acute outbreak, and the other half were inoculated with the attenuated vaccine virus (KAPEVAC). Ten, 100 or 1000 CPU/0.1 ml virus were given on days 12 and 20 of incubation. Embryos that died and surviving embryos killed at 5-day intervals were examined by light and electron microscopy. The yolk and the serum of embryos that survived until hatching were assayed for antibody content. Lymphocytes separated from the blood were used for the immuno-rosette formation and lymphocyte stimulation tests. Pathomorphological changes indicative of virus replication occurred in the liver, kidney, myocardium, gizzard muscle and chorioallantoic membrane (CAM) of the embryos in the case of both virus strains. The time of onset and severity of these changes and the time and rate of embryonic mortality depended on the virulence of the strain used for inoculation, the virus dose and the time of inoculation. Virus-neutralizing (VN) antibodies were demonstrable neither in the yolk nor in the serum of goslings exsanguinated after hatching. The lymphocytes recognized the virus antigen in the in vitro cellular tests and responded to it with blastogenic transformation. As opposed to adult birds, in the embryos duck plague virus infection did not cause damage to the digestive tract mucosa and the lymphoid organs.
authors.affiliation: Central Veterinary Institute, Budapest, Hungary.
authors.forename: R
authors.initials: R
authors.lastname: Glávits
authors.forename: F
authors.initials: F
authors.lastname: Rátz
authors.forename: E
authors.initials: E
authors.lastname: Sághy
authors.forename: M
authors.initials: M
authors.lastname: Meder
authors.forename: E
authors.initials: E
authors.lastname: Molnár
authors.forename: E
authors.initials: E
authors.lastname: Ivanics
authors.forename: E
authors.initials: E
authors.lastname: Szabó
mesh_terms: D000818:Animals; D005315:Fetal Diseases / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D005777:Geese / Q000196:embryology*; D006564:Herpesviridae / Q000276:immunology*; D006566:Herpesviridae Infections / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D011201:Poultry Diseases / Q000276:immunology / Q000473:pathology*