Confocal fluorescence microscopy in modern cell biology.
Confocal fluorescence microscopy has become a major tool in modern cell biology. The paper explains the basic principles and especially the depth discrimination properties of confocal microscopy. An important application is described briefly and outlined with some figures. The paper concludes with remarks on features to be expected in the near future.
Stelzer
E H
EH
European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
Wacker
I
I
De Mey
J R
JR
D000818:Animals; D005455:Fluorescent Antibody Technique; D007091:Image Processing, Computer-Assisted; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000379:methods*; D012680:Sensitivity and Specificity