Determination of the molecular nature and cellular localization of Thy-1 in human renal tissue.
The molecular nature and cellular localization of Thy-1 antigen in human renal tissue were studied. Strong immunohistochemical staining was observed in frozen sections of human kidney using monoclonal anti-human Thy-1 antibody; this reaction was almost completely abolished by pretreating the kidney section with phosphatidyl inositol (PI)-specific phospholipase C (PI-PLC). Immunohistochemical analysis revealed that the Thy-1 antigen is localized on the proximal tubular epithelial cells and the Bowman's capsule of the glomerulus. Northern blot analysis of renal mRNA using a cloned human Thy-1 gene revealed the presence of human Thy-1 mRNA of a similar size to the one in human brain. When a human kidney cDNA library was screened with the same probe, a cDNA of human Thy-1 was isolated. Moreover, human Thy-1 protein with a molecular weight (MW) of 21,000 was detected in renal tissue by gel electrophoresis and Western blot analysis using monoclonal anti-human Thy-1 antibody. These data demonstrate for the first time the production of human Thy-1 as a PI-anchored protein with a unique cellular location in human renal tissue.
Miyata
T
T
Department of Immunology, Nagoya University School of Medicine, Japan.
Isobe
K
K
Dawson
R
R
Ritter
M A
MA
Inagi
R
R
Oda
O
O
Taguchi
R
R
Ikezawa
H
H
Inoue
I
I
Seo
H
H
D000328:Adult; D000954:Antigens, Surface / Q000032:analysis*; D001483:Base Sequence; D015153:Blotting, Western; D006801:Humans; D007668:Kidney / Q000276:immunology*; D007678:Kidney Glomerulus / Q000276:immunology; D007684:Kidney Tubules / Q000276:immunology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D018800:Thy-1 Antigens