title: Purification of a 20 kDa phosphoprotein from epithelial cells and identification as a myosin light chain. Phosphorylation induced by enteropathogenic Escherichia coli and phorbol ester.
abstract: Previous studies on the mechanism of enteropathogenic Escherichia coli (EPEC) infection have revealed an increase in the phosphorylation state of a number of proteins in human laryngeal HEp-2 cells. The most prominent was an acidic phosphoprotein(s) of Mr 20-21 kDa. The present study reports: (a) a simple method for purification of phosphorylated 20 kDa protein; (b) identification of the 20 kDa phosphoprotein as myosin light chain; and (c) that the phorbol ester, TPA, also increased the phosphorylation of the 20 kDa myosin light chain. In contrast to the effects of EPEC, TPA stimulation resulted in the dissociation of myosin from the cytoskeleton to the cytosol.
authors.affiliation: Laboratory of Protein Structure, National Institute for Medical Research, Mill Hill, London, UK.
authors.forename: H A
authors.initials: HA
authors.lastname: Manjarrez-Hernandez
authors.forename: B
authors.initials: B
authors.lastname: Amess
authors.forename: L
authors.initials: L
authors.lastname: Sellers
authors.forename: T J
authors.initials: TJ
authors.lastname: Baldwin
authors.forename: S
authors.initials: S
authors.lastname: Knutton
authors.forename: P H
authors.initials: PH
authors.lastname: Williams
authors.forename: A
authors.initials: A
authors.lastname: Aitken
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002478:Cells, Cultured; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D006801:Humans; D009218:Myosins / Q000378:metabolism*; D010703:Phorbol Esters / Q000494:pharmacology*; D010750:Phosphoproteins / Q000302:isolation & purification* / Q000378:metabolism; D010766:Phosphorylation; D011233:Precipitin Tests
chemical_list: D000596:Amino Acids; D010703:Phorbol Esters; D010750:Phosphoproteins; D009218:Myosins