title: Solubilization and partial purification of squalene synthase from daffodil microsomal membranes.
abstract: A squalene synthase was solubilized from daffodil (Narcissus pseudonarcissus L.) microsomes with CHAPS, a zwitterionic non-denaturating detergent. By successive chromatography on DEAE Sephacel and APP Sepharose a fraction enriched in this enzyme (21-fold) was prepared.
authors.affiliation: Laboratoire de Physiologie Cellulaire Végétale, Université de Bordeaux, Talence, France.
authors.forename: L
authors.initials: L
authors.lastname: Belingheri
authors.forename: P
authors.initials: P
authors.lastname: Beyer
authors.forename: H
authors.initials: H
authors.lastname: Kleinig
authors.forename: M
authors.initials: M
authors.lastname: Gleizes
mesh_terms: D002793:Cholic Acids; D002848:Chromatography, DEAE-Cellulose; D003902:Detergents; D013186:Farnesyl-Diphosphate Farnesyltransferase / Q000302:isolation & purification* / Q000378:metabolism; D007425:Intracellular Membranes / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology*; D010713:Phosphatidylcholines / Q000378:metabolism; D010944:Plants / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D002793:Cholic Acids; D003902:Detergents; D010713:Phosphatidylcholines; D013186:Farnesyl-Diphosphate Farnesyltransferase; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate