In vivo monitoring of catecholaminergic metabolism in the C1 region of rat medulla oblongata: a comparative study by voltammetry and intracerebral microdialysis.
In vivo voltammetry or microdialysis was used to monitor catecholaminergic metabolism in the C1 region of the ventrolateral medulla oblongata of anesthetized rats. In vivo voltammetry allowed the recording of a catechol oxidation current (CA.OC) peak in this region. This CA.OC was suppressed after inhibition of monoamine oxidase by pargyline or after inhibition of tyrosine hydroxylase by alpha-methyl-p-tyrosine and was markedly increased after blockade of dopamine-beta-hydroxylase by FLA 63. Similar results were found when intracerebral microdialysis coupled with HPLC and electrochemical detection was used to measure the concentration of 3,4-dihydroxyphenylacetic acid (DOPAC) in the dialysates obtained from the C1 region: The changes in CA.OC and DOPAC concentration in the dialysates exhibited very similar kinetic characteristics in the three pharmacological experiments. These results support the involvement of DOPAC as a major component of the electrochemical signal recorded by voltammetry in the C1 group of adrenergic neurons.
Lambás-Señas
L
L
Laboratoire de Neuropharmacologie, CNRS UMR 105, Faculté de Pharmacie, Université Claude Bernard, Lyon, France.
Gillon
J Y
JY
Bouilloux
J P
JP
Seccia
M
M
Buda
M
M
Renaud
B
B
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism; D003956:Dialysis / Q000379:methods; D004299:Dopamine beta-Hydroxylase / Q000037:antagonists & inhibitors; D004594:Electrophysiology; D008297:Male; D008526:Medulla Oblongata / Q000378:metabolism*; D008991:Monitoring, Physiologic / Q000379:methods*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D014446:Tyrosine 3-Monooxygenase / Q000037:antagonists & inhibitors
D002395:Catecholamines; D002396:Catechols; D008996:Monoamine Oxidase Inhibitors; D015102:3,4-Dihydroxyphenylacetic Acid; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; C034221:catechol