title: Presence of a transposon-like element in the promoter region of an inactive patatin gene in Solanum tuberosum L.
abstract: The promoter of the PGT3 patatin gene belonging to the class II subfamily is highly homologous to other class II patatin genes except for a 736 bp insertion in front of the putative transcription start site. The insertion is characterized by structural features resembling a transposable element such as an 11 bp inverted repeat at the termini and an 8 bp duplication flanking the insertion site. Despite the high homology to active patatin genes, fusion of its promoter to the beta-glucuronidase reporter gene does not lead to detectable beta-glucuronidase (GUS) activity in transgenic potato or tobacco plants, suggesting that the inactivation of this gene might be caused by the insertion of the transposon like element.
authors.affiliation: IGF Berlin, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Köster-Töpfer
authors.forename: W B
authors.initials: WB
authors.lastname: Frommer
authors.forename: M
authors.initials: M
authors.lastname: Rocha-Sosa
authors.forename: L
authors.initials: L
authors.lastname: Willmitzer
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D002265:Carboxylic Ester Hydrolases; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004251:DNA Transposable Elements; D017343:Genes, Plant; D008961:Models, Structural; D008969:Molecular Sequence Data; D005810:Multigene Family; D009690:Nucleic Acid Conformation; D010940:Plant Proteins / Q000235:genetics*; D010947:Plants, Toxic; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D011198:Solanum tuberosum / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics; D014158:Transcription, Genetic; D014170:Transformation, Genetic
chemical_list: D004251:DNA Transposable Elements; D010940:Plant Proteins; C043222:patatin protein, Solanum tuberosum; D004247:DNA; D002265:Carboxylic Ester Hydrolases