[Treatment of pneumocystosis: preclinical evaluation].
There are now three methods to evaluate treatments of pneumocystosis: (1) the animal model, using adult rats under corticosteroids; the intensity of infection is assessed on pulmonary appositions and can then be evaluated after administration of a potential treatment compared with a reference; (2) the in vitro propagation of P. carinii observed from rat lung extracts cultured on cell lines; this method, however, is limited by the difficulties encountered in quantifying the parasitic types; (3) the inhibition by the drugs tested of enzymes involved in folate synthesis is a method that is still under evaluation. The animal model remains the reference method.
Girard
P M
PM
INSERM U 13, Hôpital Claude-Bernard, Paris.
Brun-Pascaud
M
M
Roux
P
P
D000818:Animals; D002478:Cells, Cultured; D002986:Clinical Trials as Topic / Q000379:methods*; D004195:Disease Models, Animal; D005492:Folic Acid / Q000096:biosynthesis; D006801:Humans; D011010:Pneumocystis / Q000187:drug effects / Q000201:enzymology; D011020:Pneumonia, Pneumocystis / Q000188:drug therapy*; D051381:Rats; D011919:Rats, Inbred Strains; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000627:therapeutic use
D015662:Trimethoprim, Sulfamethoxazole Drug Combination; D005492:Folic Acid
Traitement de la pneumocystose: évaluation préclinique.