Synthesis and purification of radioactive fatty acylcarnitines of high specific activity.
Micromole quantities of radiolabeled fatty acylcarnitines of high specific activity were synthesized by a simple one-step, one-pot acylation of [3H]carnitine in the presence of the respective fatty acid and acid chloride. The acylated product was isolated in high yield and high purity using liquid-liquid partitioning with isopropanol:hydrochloric acid:heptane (4:1:12 v/v) followed by butanol extraction. Product recovery was 91% for octanoyl[3H]carnitine and 90% for palmitoyl[3H]carnitine and contamination of both products with carnitine was less than 1%. The method is simple and fast, and results in high yields of pure fatty acylcarnitine in micromole scale.
Squire
R S
RS
Laboratory of Intermediary Metabolism, Clinical Research Institute of Montreal, Quebec, Canada.
D000433:1-Propanol; D002331:Carnitine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000302:isolation & purification; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002852:Chromatography, Ion Exchange; D006536:Heptanes; D006851:Hydrochloric Acid; D010172:Palmitoylcarnitine / Q000138:chemical synthesis* / Q000302:isolation & purification; D014316:Tritium
D006536:Heptanes; D014316:Tritium; D010172:Palmitoylcarnitine; D000433:1-Propanol; D006851:Hydrochloric Acid; C008698:octanoylcarnitine; D002331:Carnitine