Patterns of DNA methylation--evolutionary vestiges of foreign DNA inactivation as a host defense mechanism. A proposal.
The proposals in this review are based on experimental work on the integration of foreign DNA in mammalian cells, on the establishment of specific de novo patterns of DNA methylation, and on the inhibition of transcription by the sequence-specific methylation of promoter sequences. It is suggested that eukaryotic cells have developed several mechanisms of defense against the uptake, integration, and continued expression of foreign DNA. In the course of evolution and continuing at present, cells have been exposed to foreign DNA, entire genomes or fragments of them. A particularly problematic organ system in that respect must be digestive tract in higher organisms. The defense mechanisms are thought to be the following: (i) degradation and/or excretion of foreign DNA; (ii) excision and loss of previously integrated DNA from the host genome; (iii) targeted inactivation of foreign genes by sequence-specific methylation. Genes whose products could be advantageous to the transformed cells can somehow be selectively excluded from this silencing mechanism. In part, the specificity of de novo methylation must reside in the DNA methyltransferase systems of the host cell. However, nucleotide sequence, structure, and chromatin arrangement in the foreign DNA could also play an important role. Since defense processes must have been activated many times in evolution, patterns of DNA methylation as they can be observed today, may represent vestiges of evolution, i.e. the sum total of selective de novo methylations, possibly demethylations, and mutations.(ABSTRACT TRUNCATED AT 250 WORDS)
Doerfler
W
W
Institut für Genetik, Universität zu Köln.
D000818:Animals; D005075:Biological Evolution; D004247:DNA / Q000378:metabolism*; D008745:Methylation
D004247:DNA