CD4 rat x rat and mouse x rat T cell hybridomas produced by fusion of established T cell lines and clones to W/Fu (C58NT)D.
Previously, fusion of established T cell lines or clones has been claimed to be difficult. We now report our experiences in the fusion of both long term cultures of rat T cell clones and mouse T cell lines to rat W/Fu (C58NT)D. Upon fusion of rat T cell clones the hybrids obtained expressed antigen specificities identical to those of the parent clones. In addition, C58 was used for interspecies hybridisation of murine T cell lines. The specificity of intra- and inter-species hybrids was maintained by subcloning. We conclude that the C58 cell line can be used to generate continuously growing monoclonal T-cell reagents of sufficient stability using both intra- and inter-species hybridisation.
Boots
A M
AM
Institute of Molecular Biology and Medical Biotechnology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
van Lierop
M J
MJ
Wauben
M H
MH
van Kooten
P J
PJ
Hensen
E J
EJ
van Eden
W
W
D000818:Animals; D015704:CD4 Antigens / Q000032:analysis; D002459:Cell Fusion; D002460:Cell Line; D002999:Clone Cells; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000502:physiology; D006825:Hybridomas; D051379:Mice; D010641:Phenotype; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology*
D015704:CD4 Antigens; D000949:Histocompatibility Antigens Class II