title: A cDNA encoding 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Solanum tuberosum L.
abstract: A cDNA encoding potato (Solanum tuberosum L.) 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase, the first enzyme of the shikimate pathway, was cloned into phage lambda gt11. The clone represents the first cDNA for this enzyme from any eukaryotic source. The nucleotide sequence of the cDNA was determined, and its identity was confirmed through partial amino acid sequence analysis of the encoded enzyme. The cDNA contains a 1527-base pair open reading frame that encodes a polypeptide with a calculated molecular weight of 56,153. The amino terminus of the deduced polypeptide resembles a chloroplast transit sequence. Amino acid sequence identities between the mature potato enzyme and the homologous isoenzymes from Escherichia coli are only about 22%. The potato cDNA hybridized to various plant mRNAs that are all about 2 kilobases in size.
authors.affiliation: Department of Horticulture, Purdue University, West Lafayette, Indiana 47907.
authors.forename: W E
authors.initials: WE
authors.lastname: Dyer
authors.forename: L M
authors.initials: LM
authors.lastname: Weaver
authors.forename: J M
authors.initials: JM
authors.lastname: Zhao
authors.forename: D N
authors.initials: DN
authors.lastname: Kuhn
authors.forename: S C
authors.initials: SC
authors.lastname: Weller
authors.forename: K M
authors.initials: KM
authors.lastname: Herrmann
mesh_terms: D010723:3-Deoxy-7-Phosphoheptulonate Synthase / Q000235:genetics*; D000446:Aldehyde-Lyases / Q000235:genetics*; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002474:Cell-Free System; D002736:Chloroplasts / Q000378:metabolism; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010944:Plants / Q000201:enzymology*; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000378:metabolism; D013403:Sugar Phosphates / Q000096:biosynthesis
chemical_list: D000596:Amino Acids; D011498:Protein Precursors; D013403:Sugar Phosphates; C013469:3-deoxy-D-arabino-heptulosonate-7-phosphate; D004247:DNA; D010723:3-Deoxy-7-Phosphoheptulonate Synthase; D000446:Aldehyde-Lyases