Structural determination of D-mannans of pathogenic yeasts Candida stellatoidea type I strains: TIMM 0310 and ATCC 11006 compared to IFO 1397.
The structures of the cell-wall D-mannans of pathogenic yeasts of Candida stellatoidea Type I strains, IFO 1397, TIMM 0310, and ATCC 11006, were investigated by mild acid and, alkaline hydrolysis, by digestion with the Arthrobacter GJM-1 strain exo-alpha-D-mannosidase, and by acetolysis. The modified D-mannans and their degradation products were studied by 1H- and 13C-n.m.r. analyses. D-Manno-oligosaccharides released by acid treatment from the parent D-mannans were identified as the homologous beta-(1----2)-linked D-manno-oligosaccharides from biose to hexaose, whereas those obtained by alkaline degradation were the homologous alpha-(1----2)-linked D-mannobiose and D-mannotriose. The acid- and alkali-modified D-mannans lacking 1H-n.m.r. signals above 4.900 p.p.m. [corresponding to beta-(1----2)-linked D-mannopyranose units] were acetolyzed with 10:10:1 (v/v) Ac2O-AcOH-H2SO4, and the resultant D-manno-oligosaccharides were also analyzed. It was found that the longest branches of these D-mannans, corresponding to hexaosyl residues, had the following structures: alpha-D-Manp-(1----3)-alpha-D-Manp-(1----2)-alpha-D-Manp+ ++-(1----2)-alpha-D-Manp- (1----2)-alpha-D-Manp-(1----2)-D-Man and alpha-D-Manp-(1----2)-alpha-D-Manp-(1----3)-alpha-D-Manp+ ++-(1----2)-alpha-D-Manp- (1----2)-alpha-D-Manp-(1----2)-D-Man. These results indicate that the D-mannans of C. stellatoidea Type I strains possess structures in common with the D-mannans of Candida albicans serotype B strain (see ref. 4) containing phosphate-bound beta-(1----2)-linked oligo-D-mannosyl residues.
Kobayashi
H
H
Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, Miyagi, Japan.
Kojimahara
T
T
Takahashi
K
K
Takikawa
M
M
Takahashi
S
S
Shibata
N
N
Okawa
Y
Y
Suzuki
S
S
D002175:Candida / Q000737:chemistry* / Q000145:classification; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006851:Hydrochloric Acid; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D012972:Sodium Hydroxide; D013045:Species Specificity
D008351:Mannans; D012972:Sodium Hydroxide; D006851:Hydrochloric Acid