title: Immunohistochemical identification of neuron-specific enolase, synaptophysin, chromogranin and endocrine granule constituent in neuroendocrine tumours.
abstract: Immunohistochemical identification of neuroendocrine tumour markers in paraffin embedded material from 22 tumours (5 small cell carcinomas of the lung (SCCL), 12 carcinoids, 2 medullary thyroid carcinomas, 2 pheochromocytomas and one paraganglioma) with electron microscopically verified dense-core granules revealed neuron-specific enolase in all but one tumour, synaptophsin in 15/22 (2 SCCL and 6 carcinoids negative), chromogranin in 16/22 (all SCCL and one carcinoid negative), and endocrine granule constituent in 18/22 (4 SCCL negative). The Grimelius silver methods stained 13/22 (all cases of SCCL and 4 carcinoids negative).
authors.affiliation: Department of Pathology, Hvidovre Hospital, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Vyberg
authors.forename: T
authors.initials: T
authors.lastname: Horn
authors.forename: D
authors.initials: D
authors.lastname: Francis
authors.forename: J
authors.initials: J
authors.lastname: Askaa
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D014408:Biomarkers, Tumor / Q000032:analysis*; D002276:Carcinoid Tumor / Q000473:pathology* / Q000648:ultrastructure; D018288:Carcinoma, Small Cell / Q000473:pathology* / Q000648:ultrastructure; D002864:Chromogranins / Q000032:analysis*; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D008565:Membrane Proteins / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010235:Paraganglioma / Q000473:pathology* / Q000648:ultrastructure; D010673:Pheochromocytoma / Q000473:pathology* / Q000648:ultrastructure; D010751:Phosphopyruvate Hydratase / Q000032:analysis*; D016708:Synaptophysin; D013964:Thyroid Neoplasms / Q000473:pathology* / Q000648:ultrastructure
chemical_list: D014408:Biomarkers, Tumor; D002864:Chromogranins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D016708:Synaptophysin; D010751:Phosphopyruvate Hydratase