Sodium/potassium adenosine triphosphatase alpha- and beta-subunit and alpha-subunit mRNA levels during mouse embryo development in vitro.
Changes in sodium/potassium adenosine triphosphatase (Na+/K+ ATPase) and Na+/K+ ATPase mRNA content during preimplantation mouse embryo development were determined. Western blotting, using polyclonal antiserum against guinea pig Na+/K+ ATPase, was used to detect changes in Na+/K+ ATPase alpha- and beta-subunit content during mouse embryo development. Total RNA from mouse embryos was analyzed using Northern and slot blots hybridized with random-primer-labeled cDNA for Na+/K+ ATPase alpha-subunit from sheep kidney. Northern blots exhibited a single mRNA band (3.65 kb) in sheep and mouse kidneys and mouse embryos. Although Na+/K+ ATPase alpha-subunit mRNA content of mouse embryos increased 45-fold between Day 1 and Day 4 of development, Na+/K+ ATPase alpha-subunit content remained constant, and beta-subunit content increased 9-fold. The Na+/K+ ATPase alpha-subunit and alpha-subunit mRNA content did not increase in a similar manner. The results suggest that, in mouse embryos, blastocoel formation is not triggered by an increase in Na+/K+ ATPase alpha-subunit content. Changes in beta-subunit content may be important in regulating Na+/K+ ATPase activity and blastocoel formation.
Gardiner
C S
CS
Department of Animal Science, Oregon State University, Corvallis 97331-6702.
Williams
J S
JS
Menino
A R
AR
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D004622:Embryo, Mammalian / Q000737:chemistry / Q000378:metabolism*; D005314:Embryonic and Fetal Development; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D000254:Sodium-Potassium-Exchanging ATPase