[The inhibitory effects of sodium paeonol sulfate on calcium influx in the cultured neonatal rat heart cells during calcium paradox].
The calcium paradox model was made in cultured neonatal rat heart cells. The 45Ca uptake by the cultured neonatal rat heart cells and the contents of sialic acid (SA) of the cultured heart cells were measured to observe and evaluate the protective effects of sodium paeonol sulfate against calcium paradox in the cultured heart cells. The results demonstrated that sodium paeonol sulfate 50-400 micrograms/ml remarkably inhibited the rapid (5 min) and the slow (120 min) phases of 45Ca uptake by the cultured heart cells and myocardial beating rate (MBR) under normal states. Besides inhibition of 45Ca uptake by the cultured heart cells, this drug was found to elevate the contents of SA in the membrane of the cultured heart cells during calcium paradox. This indicates that the mechanism of protection against calcium paradox by sodium paeonol sulfate may be associated with blocking the slow calcium channel as well as increasing SA in the cell membrane.
Tang
J R
JR
Department of Pharmacology, Weifang Medical College.
Shi
L
L
D000098:Acetophenones / Q000494:pharmacology*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D008297:Male; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012794:Sialic Acids / Q000378:metabolism
D000098:Acetophenones; D012794:Sialic Acids; C071573:sodium paeonol sulfate; D002118:Calcium