Recombinant plasmid expressing the entire coding region of the Bmyc putative protein.
An expression vector with the entire coding region of Bmyc oncogene was constructed. The longest predicted open reading frame of Bmyc (178 amino acid residues) was expressed as a fusion protein with the trpE protein (308 amino acid residues) in transformed bacteria. The newly synthesized 58 Kd protein reacted with an anti pan-myc serum. The fusion protein isolated from a preparative Western blot was used as immunogen to generate rabbit anti myc specific immune sera.
Steinitz
M
M
Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
Ingvarsson
S
S
Asker
C
C
Sümegi
J
J
Klein
G
G
D000818:Animals; D015139:Blotting, Southern; D015153:Blotting, Western; D004926:Escherichia coli; D016259:Genes, myc; D005822:Genetic Vectors; D007106:Immune Sera; D016366:Open Reading Frames; D010957:Plasmids; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology
C076792:Bmyc protein, rat; D007106:Immune Sera; D016271:Proto-Oncogene Proteins c-myc; D011993:Recombinant Fusion Proteins