title: Pilins from the B serogroup of Bacteroides nodosus: characterization, expression, and cross-protection.
abstract: Sequences of pilin genes from four strains of serogroup B of the ovine pathogen Bacteroides nodosus have been determined. These sequences permit comparisons of amino acid sequence between pilins from different subtypes (B1, B2, B3, B4) of the B serogroup and assessment of intraserogroup variation. Pili of B. nodosus strains 234 (B1) and 183 (B2) were produced by Pseudomonas aeruginosa harboring a plasmid-borne B. nodosus pilin gene, and these pili were used in sheep vaccination trials. Pili from strain 183 (B2) were found to be a senior antigen to pili from strains of other B subtypes, providing protection against footrot infection caused by strains of the other B subtypes. Pili of this strain are therefore the most suitable candidate for inclusion in a pilus-based vaccine. Pili of strain 234 from subtype B1, the reference strain of the B serogroup, provided poor protection against infection with other subtypes.
authors.affiliation: Division of Biotechnology, Commonwealth Scientific and Industrial Research Organisation, Victoria, Australia.
authors.forename: T C
authors.initials: TC
authors.lastname: Elleman
authors.forename: D J
authors.initials: DJ
authors.lastname: Stewart
authors.forename: K G
authors.initials: KG
authors.lastname: Finney
authors.forename: P A
authors.initials: PA
authors.lastname: Hoyne
authors.forename: C W
authors.initials: CW
authors.lastname: Ward
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000235:genetics; D001439:Bacteroides / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western; D004269:DNA, Bacterial / Q000235:genetics; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000032:analysis*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology / Q000517:prevention & control; D014611:Vaccination
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D036781:Fimbriae Proteins