Influence of an S-layer on surface properties of Bacillus stearothermophilus.
Various aspects of surface properties of the S-layer-carrying Bacillus stearothermophilus PV72 and of an S-layer-deficient mutant (strain PV72/T5) have been tested by adsorption assays on solid surfaces, electrostatic interaction chromatography and hydrophobic interaction chromatography. The adsorption assays have shown that cell adhesion of the S-layer-carrying strain was less influenced by environmental changes than it was with the S-layer-deficient mutant. Electrostatic interaction chromatography indicated that both strains have positively and negatively charged groups exposed on the cell surface but the S-layer-carrying strain reveals more positively charged groups than does the S-layer-deficient mutant. Hydrophobic interaction chromatography showed that both strains have a hydrophilic surface but that the hydrophilic properties are more pronounced with the strain lacking an S-layer.
Gruber
K
K
Zentrum für Ultrastrukturforschung, Universität für Bodenkultur, Wien, Austria.
Sleytr
U B
UB
D001422:Bacterial Adhesion; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002852:Chromatography, Ion Exchange; D005613:Freeze Etching; D001411:Geobacillus stearothermophilus / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D013499:Surface Properties