[Use of heterophilic mononucleosis antigen in the latex diagnostic test. III. Diagnostic field studies].
The aim of this study was to evaluate usefulness of latex coated with a preparation of heterophilic mononucleosis antigen for the detection of antibodies present during a course of infectious mononucleosis. Studies were performed in district serological laboratories. Sanitary-Epidemiological Stations in conditions of routine diagnostics. Studies were performed on 656 blood serum samples collected from individuals with a clinical suspicion of infectious mononucleosis. Latex and Paul-Bunnell-Davidsohn (PBD) tests were run in parallel. Out of 154 blood serum samples which contained antibodies detected by PBD test in diagnostically significant titer of 1:56 or higher, 151 were reactive in latex test in the dilution 1:5 or higher, while in the remaining three cases agglutination appeared with undiluted serum only. Out of 268 serum samples tested in latex test 167 reacted in 1:5 titer - diagnostically accepted as a significant or in higher titers. The sensitivity of latex test amounted to 98.1% and specificity was 96.9% as compared to reference Paul-Bunnell-Davidsohn test. The results of our study suggest the possibility of replacing Paul-Bunnell-Davidsohn test by latex test performed as a method providing the possibility of determination of the presence of heterohilic antibodies in blood serum samples and the follow up of their dynamics.
Jagielski
M
M
Zakład Bakteriologii PZH w Warszawie.
Kałuzewski
S
S
D000914:Antibodies, Viral / Q000032:analysis*; D015478:Antigens, Heterophile / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007244:Infectious Mononucleosis / Q000175:diagnosis* / Q000276:immunology; D007841:Latex Fixation Tests / Q000379:methods*; D011044:Poland
D000914:Antibodies, Viral; D015478:Antigens, Heterophile; D000956:Antigens, Viral
Heterofilny antygen mononukleozowy w zastosowaniu do lateksowego testu diagnostycznego. III. Terenowe badania diagnostyczne.