[Effect of retinoic acid activated mouse macrophage on human alveolar cell carcinoma of lung(A549) in vitro].
The tumoricidal activity of mouse macrophage (M phi) activated with retinoic acid (RA) and the effect of RA in combination with CP on mouse M phi were studied with phase contrast microscopy, light microscopy, and 3HTdR labelling technique. The results of our studies suggest that: (1) RA can activate mouse peritoneal M phi both in vivo and in vitro, and the RA-activated M phi are cytostatic and cytolytic to A549 cells. The degree of activation is related to the concentration or dosage of RA given, and (2) RA can enhance the function of CP-activated M phi. A summation effect was obtained by giving RA in combination with CP. This effect was related to the dosage of RA. When optimal dosage was given in combination with CP, a synergistic action in activating M phi could be obtained. Morphological changes could be seen under phase contrast microscope and light microscope in killed tumor cells.
Zhang
X H
XH
Department of Pathology, Shanghai School Medical University.
D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000473:pathology*; D000818:Animals; D001428:Bacterial Vaccines / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D004305:Dose-Response Relationship, Drug; D005260:Female; D008175:Lung Neoplasms / Q000473:pathology*; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D010529:Peritoneal Cavity; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D001428:Bacterial Vaccines; D014212:Tretinoin