[Poly(U)-dependent interaction of yeast tRNA(Phe) and its fragments with ribosomes from Escherichia coli].
The poly(U)-dependent bindings of yeast tRNAPhe, its derivative depleted of 3'-terminal adenosine, and 15-nucleotide having a sequence of yeast tRNAPhe anticodon arm to the P site of Escherichia coli 70S ribosomes were compared. The equilibrium and rate constants were determined. Data indicate that the anticodon arm (N28-N42) contributes the major fraction of the binding free energy (-45.3 kJ/mol at 10 mM Mg2+ and 30 degrees C). Other parts of the tRNAPhe molecule besides A76 (N1-N27 and N43-N75) bring additional-6.0 kJ/mol, and A76 contributes-2.4 kJ/mol.
Parfenov
D V
DV
Saminskiĭ
E M
EM
D004926:Escherichia coli / Q000166:cytology*; D007700:Kinetics; D011072:Poly U / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D013816:Thermodynamics; D015003:Yeasts / Q000378:metabolism*
D012360:RNA, Transfer, Phe; D011072:Poly U
Poli(U)-zavisimoe vzaimodeĭstvie drozhzhevoĭ tRNK(Phe) i ee fragmentov s ribosomami iz Escherichia coli.