Determination of sodium diethyldithiocarbamate (Imuthiol) and its S-methyl metabolite by gas chromatography-mass spectrometry. Use of deuteromethyl iodide derivatization.
A gas-chromatographic-mass spectrometric method is described to measure the plasma concentration of sodium diethyldithiocarbamate (ditiocarb sodium, DEDTC-Na), the active ingredient of Imuthiol, a drug found to be active in the opportunistic infections occurring in AIDS, and its S-methyl metabolite. Plasma samples are treated with deuteromethyl iodide and DEDTC-Na is transformed into its deuteromethyl ester, which is then co-extracted with the S-methyl metabolite. Gas chromatography-mass spectrometry and selected-ion monitoring allow the specific determination of both compounds. Linear calibration curves were obtained up to 4000 ng/ml. This method has been successfully applied for pharmacokinetic studies after Imuthiol and disulfiram, the dimer of DEDTC, were administered to humans.
Scappaticci
B
B
L.E.A.C.M. Laboratoire d'Etudes Analytiques et Cinetiques du Medicament, Institut des Sciences Pharmaceutiques et Biologiques, Lyon, France.
Soubeyrand
J
J
Rousseau-Tsangaris
M
M
Desage
M
M
Brazier
J L
JL
Coquet
B
B
D003903:Deuterium; D004221:Disulfiram / Q000493:pharmacokinetics; D004050:Ditiocarb / Q000032:analysis* / Q000493:pharmacokinetics / Q000031:analogs & derivatives; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans
C016571:methyl diethyldithiocarbamate; D004050:Ditiocarb; D003903:Deuterium; D004221:Disulfiram