Cloning of a serotonin transporter affected by antidepressants.
A complementary DNA clone for a serotonin (5HT) transporter has been isolated from rat basophilic leukemia cells. The complementary DNA sequence predicts a 653-amino acid protein with 12 to 13 putative transmembrane domains. The 5HT transporter has significant homology to the gamma-aminobutyric acid, dopamine, and norepinephrine transporters. Uptake by CV-1 cells expressing the transporter complementary DNA resembles 5HT uptake by platelets and brain synaptosomes; it is sensitive to antidepressants, amphetamine derivatives, and cocaine.
Hoffman
B J
BJ
Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, MD 20892.
Mezey
E
E
Brownstein
M J
MJ
D000595:Amino Acid Sequence; D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D001483:Base Sequence; D002352:Carrier Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D007700:Kinetics; D015471:Leukemia, Basophilic, Acute; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D014162:Transfection
D000928:Antidepressive Agents; D002352:Carrier Proteins; D015345:Oligonucleotide Probes; C025366:serotonin-binding protein; D012701:Serotonin