Expression of rotavirus VP7 antigens in fusions with bacterial proteins.
We have cloned antigenic regions of the VP7 gene from rotavirus RV-5 into the lamB gene. The insertions discussed in this paper comprise 250bp of rotavirus DNA and cover the A and B antigenic regions of the protein. The fusion proteins are expressed and are present in the outer membrane: they react with anti-rotavirus antibody. However, as yet we have not been able to demonstrate that the fusion has LamB protein functions with regard to maltodextrins or L phage, and the fusion protein appears to exist in the outer membrane as a monomer rather than as a trimer.
Reeves
P R
PR
Department of Microbiology, University of Sydney, NSW, Sydney, Australia.
Johnson
M A
MA
Holmes
I H
IH
Dyall-Smith
M L
ML
D000956:Antigens, Viral / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins; D010582:Bacteriophage lambda / Q000378:metabolism; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins; D011134:Polysaccharides / Q000378:metabolism; D018272:Porins; D011487:Protein Conformation; D011991:Receptors, Virus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012401:Rotavirus / Q000235:genetics / Q000276:immunology*
D000956:Antigens, Viral; D001425:Bacterial Outer Membrane Proteins; D036022:Capsid Proteins; D011134:Polysaccharides; D018272:Porins; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; C050779:VP7 protein, Rotavirus; C016532:maltoporins; C008315:maltodextrin