DNA-binding protein RAP1 stimulates meiotic recombination at the HIS4 locus in yeast.
In the yeast Saccharomyces cerevisiae, as in other eukaryotes, some regions of the genome have a much higher rate of meiotic recombination than others. We show below that the binding of the RAP1 protein to a site upstream of the HIS4 gene is necessary for a high rate of meiotic (but not mitotic) recombination at this locus. A mutation in the RAP1 binding site at HIS4 results in a decrease in recombination; overproduction of RAP1 causes an increase in recombination at HIS4 above wild-type levels.
White
M A
MA
Department of Biology, University of North Carolina, Chapel Hill 27599-3280.
Wierdl
M
M
Detloff
P
P
Petes
T D
TD
D001483:Base Sequence; D001665:Binding Sites; D004271:DNA, Fungal / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000502:physiology*; D005785:Gene Conversion; D005800:Genes, Fungal; D006639:Histidine; D008540:Meiosis; D008938:Mitosis; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D011995:Recombination, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D020668:rap GTP-Binding Proteins
D004271:DNA, Fungal; D009841:Oligonucleotides; D006639:Histidine; D019204:GTP-Binding Proteins; D020668:rap GTP-Binding Proteins