Pretreatment with N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline accelerates the electroshock-induced decrease in density of beta-adrenoceptors but not functional downregulation.
Intrapritoneal injection of N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ) to rats significantly reduced the density of alpha 1- and alpha 2-adrenoceptors in the cerebral cortex, without affecting beta-adrenoceptors. A single dose of EEDQ markedly accelerated the development of downregulation of beta-adrenoceptors induced by a short series of electroshocks. The accumulation of cAMP, induced by isoproterenol, was unchanged in rats treated with EEDQ, while the effect of noradrenaline was reduced, to the level observed after isoproterenol alone, indicating the attenuation of alpha-adrenoceptor function. The isoproterenol-induced accumulation of cAMP was not changed by EEDQ nor electroshock, not by the combined treatment. The stimulatory action of noradrenaline was reduced after EEDQ alone or in combination with electroshock, but the effect of electroshock alone was insignificant. The results suggest that the acceleration of downregulation of beta-adrenoceptors after combined treatment with an alpha-adrenoceptor blocking agent is related to elimination of the alpha-adrenergic potentiation of accumulation of cAMP mediated by beta-adrenoceptors.
Pilc
A
A
Institute of Pharmacology, Polish Academy of Sciences, Kraków.
Vetulani
J
J
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology*; D000818:Animals; D002395:Catecholamines / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D000242:Cyclic AMP / Q000096:biosynthesis / Q000378:metabolism; D004082:Dihydroalprenolol; D015536:Down-Regulation / Q000187:drug effects*; D004597:Electroshock; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D011804:Quinolines / Q000494:pharmacology*; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000187:drug effects*
D000317:Adrenergic alpha-Antagonists; D002395:Catecholamines; D011804:Quinolines; D011943:Receptors, Adrenergic, beta; D004082:Dihydroalprenolol; C000470:EEDQ; D000242:Cyclic AMP; D007545:Isoproterenol