Infrequent mutations in the P53 gene in primary human T-cell acute lymphoblastic leukemia.
Alterations of the P53 tumor suppressor gene are present in various human malignancies. P53 mutations have recently been detected in 60% of human T-cell leukemia permanent cell lines. To determine the frequency of P53 mutations in primary T-cell acute lymphoblastic leukemia (T-ALL), a powerful method for the detection of structural alterations of DNA was used, namely, single-strand conformation polymorphism analysis of DNA fragments amplified by the polymerase chain reaction. No point mutation in the P53 gene was shown in any of the 30 T-ALL patients tested. Unlike T-cell leukemia permanent cell lines, P53 mutations are uncommon in T-ALL.
Jonveaux
P
P
Institut de Génétique Moléculaire, U-301 INSERM, Paris, France.
Berger
R
R
D001483:Base Sequence; D002886:Chromosomes, Human, Pair 17 / Q000502:physiology; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D004277:DNA, Single-Stranded / Q000032:analysis / Q000235:genetics; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*
D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded