[Change in the passive influx of Ca2+ into sarcoplasmic reticulum vesicles as a result of chemical modification of surface amino groups].
Treatment of sarcoplasmic reticulum (SR) vesicles with trinitrobenzene (TNBS) and 1-ethyl-3(3-dimethylaminopropyl)carbodiimide (EDC) stimulates the initial rate of passive influx of Ca2+ into SR vesicles, but does not affect either the binding or the maximal passive loading of SR vesicles with Ca2+. The changes in the kinetics of KCl-stimulated passive influx of Ca2+ depend on the reagent used. It is supposed that stimulation of passive influx of Ca2+ into SR vesicles and the changes in the reaction kinetics may be caused by modification of the Ca2+ channel gating behaviour as a result of binding of surface amino groups.
Diadiusha
G P
GP
Tugaĭ
V A
VA
Zimina
V P
VP
Zakharchenko
A N
AN
D000588:Amines / Q000737:chemistry*; D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D015640:Ion Channel Gating / Q000187:drug effects; D007700:Kinetics; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D014302:Trinitrobenzenesulfonic Acid / Q000737:chemistry
D000588:Amines; D015220:Calcium Channels; D014302:Trinitrobenzenesulfonic Acid; D005022:Ethyldimethylaminopropyl Carbodiimide; D002118:Calcium
Izmenenie passivnogo vkhoda Ca2+ v vezikuly sarkoplazmaticheskogo retikuluma v rezul'tate khimicheskoĭ modifikatsii poverkhnostnykh aminogrupp.