c-myc detection in bone marrow biopsies.
In the past the fixation procedures employed in the immunohistostaining of paraffin-embedded tissues with anti-myc protein antibodies were unsatisfactory since they permitted the leakage of the protein from its normal nuclear localization in the cell. In the studies described here 3 different fixation methods were compared and it was shown that only one, the AMeX method, was suitable for studying c-myc expression in bone marrow biopsies.
Pavelic
K
K
Department of Experimental Biology and Medicine, Rudjer Boskovic Institute, Zagreb, Yugoslavia.
Pavelic
Z P
ZP
Preisler
H D
HD
D000096:Acetone; D001853:Bone Marrow / Q000648:ultrastructure*; D005189:False Positive Reactions; D005404:Fixatives; D005557:Formaldehyde; D015870:Gene Expression; D016259:Genes, myc; D006801:Humans; D007150:Immunohistochemistry; D011108:Polymers; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis
D005404:Fixatives; D011108:Polymers; D016271:Proto-Oncogene Proteins c-myc; D000096:Acetone; D005557:Formaldehyde; C003043:paraform