title: A herpesvirus maturational proteinase, assemblin: identification of its gene, putative active site domain, and cleavage site.
abstract: A herpesvirus proteinase activity has been identified and partially characterized by using the cloned enzyme and substrate genes in transient transfection assays. Evidence is presented that the proteinase gene of cytomegalovirus strain Colburn encodes a 590-amino acid protein whose N-terminal 249 residues contain the proteolytic activity and two domains that are highly conserved in the homologous protein of other herpesviruses. Insertion of a short amino acid sequence between these domains abolished proteinase activity, suggesting that this region constitutes part or all of the enzyme active site. Plasma desorption mass spectrometry was used to identify the C terminus of the mature assembly protein as alanine, enabling the recognition of a consensus proteinase cleavage sequence of V/L-X-A decreases S/V, near the C-terminal end of all herpesvirus assembly protein homologs. Interestingly, the proteinase and its substrate, the assembly protein precursor, are encoded by opposite halves of the same open reading frame.
authors.affiliation: Virology Laboratories, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
authors.forename: A R
authors.initials: AR
authors.lastname: Welch
authors.forename: A S
authors.initials: AS
authors.lastname: Woods
authors.forename: L M
authors.initials: LM
authors.lastname: McNally
authors.forename: R J
authors.initials: RJ
authors.lastname: Cotter
authors.forename: W
authors.initials: W
authors.lastname: Gibson
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002460:Cell Line; D002478:Cells, Cultured; D010450:Endopeptidases / Q000235:genetics* / Q000378:metabolism; D005347:Fibroblasts; D006564:Herpesviridae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D006801:Humans; D008954:Models, Biological; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D014176:Protein Biosynthesis; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases; D012867:Skin; D013379:Substrate Specificity; D014162:Transfection
chemical_list: D010450:Endopeptidases; D012697:Serine Endopeptidases; C071910:assemblin