Application of a novel chemiluminescence-based DNA detection method to single-vector and multiplex DNA sequencing.
A chemiluminescent DNA detection method is described and its application shown for both single-vector and multiplex DNA sequencing using the standard dideoxy chain-termination process. This recently developed detection method, which utilizes the light emitted by an enzyme-catalyzed dioxetane reaction, is highly sensitive and affords significant advantages in safety and speed over the traditional radioactive labeling method. When adapted to a multiplex strategy, this chemiluminescent detection method constitutes a safe, simple and rapid method for increasing the throughput of DNA sequencing procedures.
Creasey
A
A
New England BioLabs.
D'Angio
L
L
Dunne
T S
TS
Kissinger
C
C
O'Keeffe
T
T
Perry-O'Keefe
H
H
Moran
L S
LS
Roskey
M
M
Schildkraut
I
I
Sears
L E
LE
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005821:Genetic Techniques; D005822:Genetic Vectors / Q000235:genetics; D008163:Luminescent Measurements
D004247:DNA; D004259:DNA-Directed DNA Polymerase