Ventricular cells in culture from adult spontaneously hypertensive rats exhibit decreased growth.
We have investigated the growth characteristics of adult rat ventricular cells (ARVC) in culture from 8-week-old spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY). Protein synthesis in ARVC estimated from tritiated leucine uptake was augmented in both strains after exposure of cells to either norepinephrine or fetal bovine serum (FBS), but the response was significantly smaller in SHR than in WKY. A relative increase in the protein content of ARVC incubated with norepinephrine or FBS was also smaller in SHR than in WKY. These results demonstrate that SHR cardiac myocytes are not genetically growth-accelerated and show a decreased growth response to growth stimuli such as norepinephrine and serum during the adult period.
Ikeda
U
U
Department of Cardiology, Jichi Medical School, Tochigi, Japan.
Tsuruya
Y
Y
Kamitani
T
T
Oguchi
A
A
Oohara
T
T
Yaginuma
T
T
D000375:Aging / Q000502:physiology*; D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured / Q000473:pathology; D008297:Male; D009206:Myocardium / Q000473:pathology*; D009638:Norepinephrine / Q000494:pharmacology; D009929:Organ Size; D014176:Protein Biosynthesis; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D009638:Norepinephrine