Trophic effect of beta-amyloid precursor protein on cerebral cortical neurons in culture.
We investigated the effect of human beta-amyloid precursor protein (APP) on rat primary cerebral cortical neurons cultured in a serum-free medium. Two secretory APP species (APP667 and APP592) with and without the protease inhibitor domain were produced by COS-1 cells transfected with APP cDNAs, which encode the N-terminal portions of APP770 and APP695. Both highly purified APP species, when added to the medium, enhanced neuronal survival and neurite extension in a dose-dependent manner with a maximum effect at approximately 100 nM. These results suggest that secreted forms of APP have trophic activity for cerebral cortical neurons.
Araki
W
W
Department of Neurology, Faculty of Medicine, Kyoto University, Japan.
Kitaguchi
N
N
Tokushima
Y
Y
Ishii
K
K
Aratake
H
H
Shimohama
S
S
Nakamura
S
S
Kimura
J
J
D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000502:physiology*; D006801:Humans; D008858:Microscopy, Phase-Contrast; D008970:Molecular Weight; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D014162:Transfection
D016564:Amyloid beta-Protein Precursor; D011994:Recombinant Proteins