Progestin receptors: isoforms and antihormone action.
We present evidence that the two isoforms of A and B of the chicken (cPR) and human progesterone receptor (hPR) originate from two different mRNA populations. One of these encodes the isoforms A which originate by initiation of translation at an in-frame AUG found 127 (cPR) and 165 (hPR) codons downstream of the AUG which gives rise to the isoforms B. Two estrogen-inducible hPR promoters were identified which are responsible for the generation of these two classes of transcripts. Characterization of the cPR promoter suggested the possible existence of cell-type and isoform-specific auto-regulation of cPR transcription and provided evidence that estrogen-induction of cPR expression occurs at a post-transcriptional level. Finally, we demonstrate promoter-specific transcriptional activation by the hPR isoforms A and B, and we discuss the mechanism of action of the anti-progestin RU486.
Gronemeyer
H
H
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.
Meyer
M E
ME
Bocquel
M T
MT
Kastner
P
P
Turcotte
B
B
Chambon
P
P
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D004967:Estrogens / Q000235:genetics; D006727:Hormone Antagonists; D006801:Humans; D015735:Mifepristone / Q000494:pharmacology; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D011980:Receptors, Progesterone / Q000235:genetics*; D015183:Restriction Mapping; D014158:Transcription, Genetic
D004967:Estrogens; D006727:Hormone Antagonists; D012333:RNA, Messenger; D011980:Receptors, Progesterone; D015735:Mifepristone