Production of a cytotoxic human monoclonal antibody with specificity for HLA-DR4 and -DRw10 by cells derived from a highly sensitized kidney recipient.
A human monoclonal antibody which reacts preferentially with HLA-DR4 and -DRw10 B-cell targets has been produced. A human B-cell line, secreting antibody which reacted preferentially with DR4 and DR1 targets, was derived from a highly sensitized kidney recipient who had rejected two grafts. This line was fused with the mouse myeloma P3X63Ag8.653 and a selected hybridoma cloned. The clones secrete IgM(lambda), which reacts strongly with HLA-DR4 and -DRw10 and more weakly with -DRw14 and a proportion of -DR1 B cells in cytotoxicity assays. Using B-cell lines as targets in cytotoxicity and enzyme-linked immunosorbent assays, the antibody gives a broader pattern of reaction, reacting with HLA-DR1, -DR4, -DR9, -DRw10, -DRw14, and some -DR2 targets. The antibody (NI) is currently in use as a reagent for tissue typing.
Yendle
J E
JE
U.K. Transplant Service, Bristol.
Bowerman
P D
PD
Yousaf
K
K
Roberts
I M
IM
Cohen
B
B
Barber
L
L
Lechler
R
R
Hancock
R J
RJ
Bradley
B A
BA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D015153:Blotting, Western; D002460:Cell Line; D002469:Cell Separation; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000276:immunology*; D015804:HLA-DR4 Antigen / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D016030:Kidney Transplantation / Q000276:immunology; D051379:Mice; D012150:Polymorphism, Restriction Fragment Length; D014162:Transfection
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D015804:HLA-DR4 Antigen; C043263:HLA-DRw10