A simple method for the purification of human peripheral blood monocytes. A substitute for Sepracell-MN.
Sepracell-MN has provided a simple, rapid means of isolating peripheral blood monocytes. However this product is no longer available. Consequentially we have developed a Percoll gradient which matches Sepracell-MN in simplicity and yield of monocytes. Using this Percoll gradient, an average of 7 x 10(6) monocytes with a purity of 83% were obtained from 30-40 ml of blood. These monocytes were at least 97% viable and responded to chemotactic stimuli in comparable numbers to those prepared using Sepracell-MN.
Denholm
E M
EM
Division of Hematology, Albany Medical College, NY 12208-3479.
Wolber
F M
FM
D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009000:Monocytes / Q000166:cytology*