DNA polymorphism of the C2 and factor B gene.
C2 and factor B are encoded by very closely linked loci in the class III region of the human major histocompatibility complex on chromosome 6. The factor B gene is divided into 18 exons, and is 6 kb in length whereas the C2 gene is much larger being 18 kb in length. Sequence and Southern blot analysis has defined 5 DNA polymorphisms in the DNA encompassing the C2 and factor B loci. The most interesting of these are the SstI multi-allelic polymorphism at the 5' end and the TaqI polymorphism at the 3' end of the C2 gene. Together these allow the subdivision of haplotypes carrying the C2C and factor B F alleles. A novel combination of SstI and TaqI RFLPs is reported here.
Cross
S J
SJ
Department of Biochemistry, University of Oxford, UK.
Thomson
W
W
D000483:Alleles; D015139:Blotting, Southern; D002896:Chromosomes, Human, Pair 6; D003175:Complement C2 / Q000032:analysis / Q000235:genetics*; D011415:Complement Factor B / Q000032:analysis / Q000235:genetics*; D004586:Electrophoresis; D004792:Enzyme Precursors / Q000235:genetics*; D006680:HLA Antigens / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008285:Major Histocompatibility Complex / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D003175:Complement C2; D004792:Enzyme Precursors; D006680:HLA Antigens; D011415:Complement Factor B