Characterization of VIP- and helodermin-preferring receptors on human small cell lung carcinoma cell lines.
We investigated the molecular and pharmacologic characteristics of VIP receptors on two human SCLC cell lines: NCI-N592 and NCI-H345. With NCI-N592 cell, the order of potency of VIP-related peptides in inhibiting 125I-VIP binding and in stimulating cAMP production was typical of the human VIP receptor. By covalent cross-linking, a polypeptide of Mr 62,300 was obtained. Conversely, the behavior of NCI-H345 cell line was totally different: helodermin was the most potent peptide, VIP and PHI were equipotent, while hGRF and secretin were totally ineffective. These results suggest that NCI-N592 cells possess a typical VIP receptor while NCI-H345 cells possess a helodermin-preferring receptor, and that the natural target of helodermin might not be the VIP receptor.
Luis
J
J
University of Illinois, Chicago.
Said
S I
SI
D018288:Carcinoma, Small Cell / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003432:Cross-Linking Reagents; D000242:Cyclic AMP / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D007457:Iodine Radioisotopes; D008175:Lung Neoplasms / Q000378:metabolism*; D010455:Peptides; D011956:Receptors, Cell Surface / Q000737:chemistry*; D011964:Receptors, Gastrointestinal Hormone / Q000737:chemistry* / Q000378:metabolism; D018013:Receptors, Neuropeptide; D018005:Receptors, Vasoactive Intestinal Peptide; D014407:Tumor Cells, Cultured; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
D003432:Cross-Linking Reagents; D036341:Intercellular Signaling Peptides and Proteins; D007457:Iodine Radioisotopes; D010455:Peptides; D011956:Receptors, Cell Surface; D011964:Receptors, Gastrointestinal Hormone; D018013:Receptors, Neuropeptide; D018005:Receptors, Vasoactive Intestinal Peptide; C068446:helodermin receptor; D014660:Vasoactive Intestinal Peptide; C040442:heliodermin; D000242:Cyclic AMP