Photosensitization of cultured cells and viruses by pyrene lipids.
Administration of pyrene-linked fatty acids and lipids to cultured cells or an enveloped (vesicular stomatitis) virus induced photosensitization which, following irradiation with a long ultra-violet light (LUV), resulted in killing of the cells and loss of the infectivity of the virus with the following specific effects. (i) LUV illumination of the pyrene-sphingomyelin administered cultured skin fibroblasts derived from normal individuals and patients with Niemann-Pick disease permitted selective killing of the latter. (ii) Similarly LUV illumination of pyrenedodecanoic acid (P12) incubates of leukemic cell lines mixed with human bone marrow cells permitted selective killing of the former. (iii) LUV illumination of P12 incubates of vesicular stomatitis virus decreased the infectivity of the virus by up to 12 logs.
Gatt
S
S
Department of Membrane Biochemistry, Hebrew University, Hadassah School of Medicine, Jerusalem, Israel.
Dinur
T
T
Abou-Rabia
S
S
Kotler
M
M
Fibach
E
E
D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D002478:Cells, Cultured; D005347:Fibroblasts; D007850:Lauric Acids / Q000494:pharmacology*; D007951:Leukemia, Myeloid / Q000473:pathology; D009542:Niemann-Pick Diseases / Q000473:pathology; D011721:Pyrenes / Q000494:pharmacology*; D013109:Sphingomyelins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development*
D007850:Lauric Acids; D011721:Pyrenes; D013109:Sphingomyelins; C040596:12-(1-pyrene)dodecanoic acid