Cytotoxic T cell responses in lambs experimentally infected with bovine respiratory syncytial virus.
The role of cell-mediated immune response in the immunopathogenesis of bovine respiratory syncytial virus (BRSV) infection is not well established. In the present study, cytotoxic T cell responses of BRSV-infected lambs were examined using the chromium release assay. Lambs experimentally infected with BRSV developed cytotoxic lymphocytes in the peripheral blood and the spleen, which lysed BRSV-infected but not uninfected cells. Peak cytotoxic activity occurred 10-14 days after infection. Pretreatment of mononuclear cells with anti-CD8 monoclonal antibodies and rabbit complement significantly reduced cytotoxic activity (P less than 0.05). It appears, therefore, that lambs experimentally infected with BRSV develop virus-specific, predominantly CD8+, cytotoxic lymphocytes in the peripheral blood and spleen.
Sharma
R
R
University of Liverpool, Department of Veterinary Pathology, Neston, Wirral, UK.
Woldehiwet
Z
Z
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D016516:CD4-CD8 Ratio; D008168:Lung / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D012136:Respiratory Syncytial Viruses / Q000276:immunology* / Q000302:isolation & purification; D010253:Respirovirus Infections / Q000276:immunology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000911:Antibodies, Monoclonal