A gel electrophoresis system for resolving over 500 nucleotides with a single sample loading.
A procedure for the resolution of over 500 nucleotides in a single loading on a sequencing gel is described. The method uses exponential wedge spacers to achieve compression of the band spacing in the lower portion of the gel. The method is simple and reliable and reduces the cost of electrophoresis and autoradiography supplies by at least a factor of two.
States
J C
JC
Center for Molecular Biology, Wayne State University, Detroit, MI 48201.
Patel
L R
LR
Li
Q A
QA
D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D009711:Nucleotides
D009711:Nucleotides