Crystallization and preliminary crystallographic study of glucose dehydrogenase from the archaebacterium Thermoplasma acidophilum.
Single crystals of glucose dehydrogenase from the archaebacterium Thermoplasma acidophilum were obtained using the hanging-drop vapour diffusion method and polyethylene glycol as a precipitant in the presence of NADP+ at pH 5.4. The crystals belong to the hexagonal space group P6122 or P6522, with unit cell dimensions a = b = 121.9 angstrom, c = 229.6 angstrom and with two molecules in the asymmetric unit.
Bright
J R
JR
Department of Biochemistry, University of Bath Claverton Down, U.K.
Mackness
R
R
Danson
M J
MJ
Hough
D W
DW
Taylor
G L
GL
Towner
P
P
Byrom
D
D
D001426:Bacterial Proteins / Q000648:ultrastructure*; D003461:Crystallography; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000648:ultrastructure*; D011487:Protein Conformation; D013822:Thermoplasma / Q000201:enzymology*; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase