Mitochondrial import of the human chaperonin (HSP60) protein.
The mitochondrial import of a member of the "chaperonin" group of proteins which play an essential role in the import of protein into organelles and their subsequent proper folding has been examined. The cDNA for human hsp60 (synonyms: GroEL homolog, P1) was transcribed and translated in vitro and its import into isolated rat heart mitochondria examined. The protein was converted into a mature form of lower molecular mass (= 58 kDa) which was resistant to trypsin treatment. The import of human hsp60 into mitochondria was inhibited in the presence of an uncoupler and also no import occurred when the N-terminal presequence was lacking. These results indicate that the chaperonin protein(s) are transported into mitochondria by a process similar to other imported mitochondrial proteins. Our results also indicate that although the P1 protein precursor was efficiently imported into mitochondria, in comparison to precursors of other mitochondrial proteins (viz. ornithine carbamoyltransferase and uncoupling protein) much less binding of pre P1 to mitochondria was observed. The significance of this latter observation at present is unclear.
Singh
B
B
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.
Patel
H V
HV
Ridley
R G
RG
Freeman
K B
KB
Gupta
R S
RS
D000595:Amino Acid Sequence; D000818:Animals; D018833:Chaperonins; D003001:Cloning, Molecular; D015723:Gene Library; D006360:Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D008715:Methionine / Q000378:metabolism; D008929:Mitochondria, Heart / Q000378:metabolism*; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D014158:Transcription, Genetic
D006360:Heat-Shock Proteins; D011506:Proteins; D008715:Methionine; D018833:Chaperonins