title: Purification and properties of dipeptidyl peptidase IV from human urine.
abstract: Dipeptidyl peptidase IV (DPP-IV) (EC 3.4.14.5) has been purified from normal human urine using immunoaffinity chromatography. The purified enzyme was homogeneous as judged by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The molecular mass of urinary DPP-IV was estimated to be 280 kDa by gel filtration. The Km value for the hydrolysis of (Gly-L-Pro)-4-methylcoumaryl-7-amide tosylate was calculated to be 1.25 +/- 0.25 X 10(-4) M; the pH optimum and pI were 8.7 and 6.5, respectively. These properties were the same as those of renal DPP-IV. Both renal and this urinary DPP-IV were inhibited by diisopropylfluorophosphate and activated by phospholipid. From these results we suppose that urinary DPP-IV may be derived from the kidney rather than from the blood
authors.affiliation: Department of Pharmaceutical Analytical Chemistry, Showa College of Pharmaceutical Sciences.
authors.forename: T
authors.initials: T
authors.lastname: Chikuma
authors.forename: T
authors.initials: T
authors.lastname: Hama
authors.forename: T
authors.initials: T
authors.lastname: Nagatsu
authors.forename: M
authors.initials: M
authors.lastname: Kumegawa
authors.forename: T
authors.initials: T
authors.lastname: Kato
mesh_terms: D000328:Adult; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000652:urine*; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007668:Kidney / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D010743:Phospholipids / Q000494:pharmacology; D013379:Substrate Specificity
chemical_list: D010743:Phospholipids; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4