Evaluation of combined commercial enzyme-linked immunosorbent assay for detection of rota and adenoviruses for automation.
219 stools were examined by direct electron microscopy (EM), culture and 'combined' commercial enzyme-linked immunosorbent assay kits (CELISA). The specificity of the combined ELISA for rotavirus was 100% as compared with EM, and 100% for adenovirus when both culture in addition to EM were carried out. ELISA appeared to be more sensitive than EM for both viruses. There was no cross-reaction between the 2 'combined' antisera. This technique may be useful for automation of viral diagnosis with ELISA using a 'panel' of selected viruses for a variety of specimens.
Cheung
E Y
EY
Department of Laboratory Medicine, Royal Alexandra Hospital, Edmonton, Alberta, Canada.
Hnatko
S I
SI
Gunning
H
H
Thomas
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R
D000256:Adenoviridae / Q000302:isolation & purification* / Q000648:ultrastructure; D000818:Animals; D001322:Autoanalysis; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology*; D005260:Female; D008297:Male; D012401:Rotavirus / Q000302:isolation & purification* / Q000648:ultrastructure; D014709:Vero Cells / Q000382:microbiology