title: Origin of hematopoietic progenitor cells after bone marrow transplantation: analysis by means of a Y-chromosome specific DNA probe.
abstract: To characterize hematopoietic cells in mixed hematopoietic chimeras after allogeneic bone marrow transplantation (BMT), the authors examined the origin of progenies derived from hematopoietic progenitor cells of male recipients who received a marrow graft from female donors, by use of a Y-chromosome specific DNA (YDNA) probe in combination with an in vitro colony assay. Host-type hematopoietic cells were detected in cultured bone marrow mononuclear cells (BMMC) from 4 out of 6 patients studied, who were all in complete remission. In 2 patients of the mixed chimeras, the relative amount of host-derived YDNA from BMMC increased after methylcellulose cultures for 14 days. Analysis of individual colonies derived from granulocyte-macrophage colony forming units (CFU-GM) from these mixed chimeras, including 2 patients with chronic myelogenous leukemia (CML), revealed approximately 30% of total colonies were host-type, although no evidence for the existence of residual Ph1 positive cells was obtained by using polymerase chain reaction for detecting bcr-abl chimeric messenger RNA in the 2 CML patients. These findings provide direct evidence that considerable numbers of host-derived normal hematopoietic progenitors survive and persist for a long term in a certain population of marrow recipients, after BMT following supralethal radiochemotherapy.
authors.affiliation: Third Department of Medicine, Kanazawa University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nakatsumi
authors.forename: S
authors.initials: S
authors.lastname: Nakao
authors.forename: M
authors.initials: M
authors.lastname: Ohtaguro
authors.forename: T
authors.initials: T
authors.lastname: Chujo
authors.forename: H
authors.initials: H
authors.lastname: Tsuchiya
authors.forename: T
authors.initials: T
authors.lastname: Niki
authors.forename: S
authors.initials: S
authors.lastname: Shiobara
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: K
authors.initials: K
authors.lastname: Nagai
authors.forename: T
authors.initials: T
authors.lastname: Matsuda
mesh_terms: D016026:Bone Marrow Transplantation; D002678:Chimera / Q000235:genetics; D002999:Clone Cells; D003114:Colony-Forming Units Assay; D015342:DNA Probes; D006801:Humans; D007938:Leukemia / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D013234:Stem Cells / Q000502:physiology*; D014998:Y Chromosome
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger