A dot immunobinding assay on nitrocellulose for the detection of bovid herpesvirus-4 antibodies.
A dot immunobinding assay (DIA) was developed for the detection of antibody against bovid herpesvirus-4 (BHV-4) in bovine sera. A semipurified virus preparation was used as the antigen and an antispecies horseradish peroxidase-labeled IgG and diaminobenzidine were employed as the detection system. The sensitivity and specificity of the DIA were similar to those of indirect fluorescent antibody test, indicating the suitability of DIA as a rapid field test for the detection of anti-BHV-4 antibodies in cattle.
Naeem
K
K
Department of Veterinary Diagnostic Investigation, College of Veterinary Medicine, University of Minnesota, Saint Paul 55108.
Goyal
S M
SM
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006564:Herpesviridae / Q000276:immunology*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary; D007106:Immune Sera / Q000276:immunology; D011237:Predictive Value of Tests
D000914:Antibodies, Viral; D007106:Immune Sera