title: Cryptosin induces backbone structural changes in cardiac Na+ and K+ dependent adenosinetriphosphatase.
abstract: Cryptosin, a new cardenolide, was found to preferentially bind to Na,K-ATPase enzyme (7), which is believed to be the ouabain binding site on cardiac sarcolemmal membrane. CD spectral studies revealed that cryptosin, in the presence of Na+ and Mg++ ions, bind to Na,K-ATPase and induce a dose-dependent change in the backbone structure of cardiac Na,K-ATPase.
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Bangalore.
authors.forename: R R
authors.initials: RR
authors.lastname: Venkateswara
authors.forename: D
authors.initials: D
authors.lastname: Dipak
authors.forename: J W
authors.initials: JW
authors.lastname: Banning
authors.forename: C S
authors.initials: CS
authors.lastname: Vaidyanathan
mesh_terms: D000818:Animals; D001665:Binding Sites; D002301:Cardiac Glycosides / Q000378:metabolism / Q000494:pharmacology*; D002942:Circular Dichroism; D004285:Dogs; D005260:Female; D006168:Guinea Pigs; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D010042:Ouabain / Q000378:metabolism; D011487:Protein Conformation / Q000187:drug effects; D012964:Sodium / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism
chemical_list: D002301:Cardiac Glycosides; C060095:cryptosin; D010042:Ouabain; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D008274:Magnesium