Immunoassays for the detection of human collagenase, stromelysin, tissue inhibitor of metalloproteinases (TIMP) and enzyme-inhibitor complexes.
Immunoassays have been developed for human collagenase, stromelysin, tissue inhibitor of metalloproteinases (TIMP) and TIMP complexed with both of the active enzymes. Selection of antibodies of defined specificity enabled measurement of both the pro and active forms of the metalloproteinase. Free TIMP was quantified by the selection of a monoclonal antibody which did not recognise TIMP when complexed with metalloproteinases. Detection of enzyme-inhibitor complexes was achieved by capturing the TIMP component of the complex and revealing the metalloenzyme using specific antibodies.
Cooksley
S
S
Department of Immunochemistry, Celltech Ltd., UK.
Hipkiss
J B
JB
Tickle
S P
SP
Holmes-Ievers
E
E
Docherty
A J
AJ
Murphy
G
G
Lawson
A D
AD
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D004247:DNA / Q000235:genetics; D004789:Enzyme Activation; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology / Q000494:pharmacology; D006801:Humans; D019278:Matrix Metalloproteinase 3; D008666:Metalloendopeptidases / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000276:immunology; D003012:Microbial Collagenase / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000276:immunology; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology; D019714:Tissue Inhibitor of Metalloproteinases
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D011994:Recombinant Proteins; D019714:Tissue Inhibitor of Metalloproteinases; D004247:DNA; D008666:Metalloendopeptidases; D019278:Matrix Metalloproteinase 3; D003012:Microbial Collagenase