title: [Fundamental studies on differentiation inducers using a human embryonal carcinoma model].
abstract: A human embryonal carcinoma (EC) cell line, NEC14, was pluripotent and the tumors formed in nude mice contained differentiated somatic and extra-embryonic elements in addition to EC. The NEC14 cells could be induced to differentiate with 5 differentiation inducers in vitro, and N,N'-hexamethylene bisacetamide (HMBA) showed the most potent differentiator induction among them. All of the NEC14 cells were morphologically differentiated when exposed to 10(-2) M HMBA for 3 days. The differentiated derivatives ceased to proliferate in vitro and became non-tumorigenic in nude mouse. Several differentiation markers appeared in the differentiated derivatives. When the tumor-bearing nude mice were injected intraperitoneally with HMBA (10mg, twice a week), the tumor growth was inhibited. These results show that human EC is a useful model of differentiation-induction in cancer therapy.
authors.affiliation: Department of Obstetrics and Gynecology, Chiba University School of Medicine.
authors.forename: K
authors.initials: K
authors.lastname: Yamazawa
authors.forename: S
authors.initials: S
authors.lastname: Sekiya
authors.forename: H
authors.initials: H
authors.lastname: Kimura
authors.forename: H
authors.initials: H
authors.lastname: Shirasawa
authors.forename: M
authors.initials: M
authors.lastname: Kawata
authors.forename: H
authors.initials: H
authors.lastname: Takamizawa
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008954:Models, Biological; D009368:Neoplasm Transplantation; D013724:Teratoma / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D000081:Acetamides; D004273:DNA, Neoplasm; C014026:hexamethylene bisacetamide