title: Effects of indomethacin and PEEP on oleic acid induced pulmonary oedema in rabbits.
abstract: This study was designed to determine whether indomethacin, a suggested treatment for adult respiratory distress syndrome (ARDS), would inhibit the efficacy of positive end-expiratory pressure (PEEP) in an animal model of ARDS. Functional residual capacity (FRC), alveolar-to-arterial oxygen difference (A-aPo2) and total lung water were measured in rabbits: 30 controls and 30 with oleic acid induced pulmonary oedema. Within each group, four treatments were administered: diluent (n = 12), PEEP (n = 6), indomethacin (n = 6), or PEEP + indomethacin (n = 6). Lung injury was induced at 30 mins and treatment commenced at 45 min. Oleic acid caused a significant increase at 3 h in % increase in A-aPo2 from baseline (105 +/- 12%) attenuated by PEEP (59 +/- 17%) and indomethacin (57 +/- 7%), with the combination of PEEP + indomethacin preventing a significant increase (26 +/- 9%). PEEP increase FRC in both saline and oleic acid animals; this was not reversed by indomethacin. Oleic acid caused an increase in total lung water (5.16 to 6.58 +/- 0.16 g), not influenced by any treatment. These findings suggest that indomethacin did not inhibit the efficacy of PEEP in this model of ARDS.
authors.affiliation: Dept of Thoracic Medicine, Royal North Shore Hospital, St Leonards, N.S.W. Australia.
authors.forename: K S
authors.initials: KS
authors.lastname: Panaretto
authors.forename: C
authors.initials: C
authors.lastname: Phillips
authors.forename: N
authors.initials: N
authors.lastname: Berend
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000097:blood; D005652:Functional Residual Capacity / Q000187:drug effects; D007213:Indomethacin / Q000494:pharmacology* / Q000627:therapeutic use; D008168:Lung / Q000473:pathology; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids; D009929:Organ Size / Q000187:drug effects; D010100:Oxygen / Q000097:blood; D011175:Positive-Pressure Respiration; D011654:Pulmonary Edema / Q000139:chemically induced / Q000188:drug therapy / Q000628:therapy*; D011817:Rabbits
chemical_list: D009829:Oleic Acids; D002245:Carbon Dioxide; D019301:Oleic Acid; D010100:Oxygen; D007213:Indomethacin