Studies on selenate and selenite absorption by sheep ileum using an everted sac method and an isolated, vascularly perfused system.
1. Using everted sacs of sheep ileum, selenate uptake was shown to be more rapid than selenite uptake. 2. Sulphate, thiosulphate and ouabain all inhibited the uptake of selenate. A Na+K(+)-ATPase is probably responsible for energizing the ileal brush-border transport of selenate. 3. The isolated, vascularly perfused ileum was shown to absorb selenate more rapidly than selenite. 4. The perfusion system described would appear to be a useful tool for studying the kinetics of intestinal absorption and the measurement of metabolic transformations within the mucosa.
Turner
J C
JC
Wallaceville Animal Research Center, Ministry of Agriculture and Fisheries, Upper Hutt, New Zealand.
Osborn
P J
PJ
McVeagh
S M
SM
D000818:Animals; D007082:Ileum / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000187:drug effects / Q000502:physiology*; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D064586:Selenic Acid; D020887:Selenious Acid; D012643:Selenium / Q000378:metabolism*; D018036:Selenium Compounds; D012756:Sheep / Q000378:metabolism*; D013431:Sulfates / Q000494:pharmacology; D013885:Thiosulfates / Q000494:pharmacology
D018036:Selenium Compounds; D013431:Sulfates; D013885:Thiosulfates; D010042:Ouabain; D020887:Selenious Acid; D012643:Selenium; D064586:Selenic Acid