title: The transactivating domain of the c-Jun proto-oncoprotein is required for cotransformation of rat embryo cells.
abstract: The nuclear phosphoprotein c-Jun, encoded by the proto-oncogene c-jun, is a major component of the AP-1 complex. A potent transcriptional regulator, c-jun is also able to transform normal rat embryo cells in cooperation with an activated c-Ha-ras gene. By deletion analysis, we identified the regions of c-Jun encoding transformation and transactivation functions. Our studies indicate that there is a direct correlation between the ability of the c-Jun protein to activate transcription and cotransform rat embryo cells. The regions involved in these functions include the conserved leucine zipper/DNA binding domain and an effector domain near its N terminus. This N-terminal region spans amino acids 61 to 146 of the c-Jun protein and is highly conserved among all Jun family members. These results support the hypothesis that c-Jun transforms cells by stimulating the expression of transformation-mediating genes.
authors.affiliation: NCI-Navy Medical Oncology Branch, Bethesda, Maryland.
authors.forename: R
authors.initials: R
authors.lastname: Alani
authors.forename: P
authors.initials: P
authors.lastname: Brown
authors.forename: B
authors.initials: B
authors.lastname: Binétruy
authors.forename: H
authors.initials: H
authors.lastname: Dosaka
authors.forename: R K
authors.initials: RK
authors.lastname: Rosenberg
authors.forename: P
authors.initials: P
authors.lastname: Angel
authors.forename: M
authors.initials: M
authors.lastname: Karin
authors.forename: M J
authors.initials: MJ
authors.lastname: Birrer
mesh_terms: D000818:Animals; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004247:DNA; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D015533:Transcriptional Activation
chemical_list: D016755:Proto-Oncogene Proteins c-jun; D004247:DNA