[Human breast cancer embedded in paraffin, fixed in Bouin's fluid. Immunohistochemical analysis of estrogen and progesterone receptors].
A new immunohistochemical method using a monoclonal estrogen receptor (ER) antibody and a progesterone receptor (PR) antibody has been performed to determine hormonal receptors on Bouin's liquid fixed and paraffin embedded tissue. This technique has been evaluated by comparison with conventional immunohistochemistry on frozen sections for 100 surgical biopsy specimens of breast carcinoma Erica (C) Prica (C); in 77 of these cases it was compared with enzyme immunoassay analyses (ER-EIA, PR-EIA). After a pretreatment by proteinase K for ER, the two specific monoclonal antibodies ER or PR were incubated overnight and revealed with a streptavidin biotin complex. The specific staining for ER and PR were exclusively located in the nuclei of cancer cells in both paraffin for ER and PR were exclusively located in the nuclei of cancer cells in both paraffin and frozen sections. The results on paraffin-embedded material were compared successively with ERICA (C) and ER-EIA and with PRICA (C) and PR-EIA. In 100 breast infiltrating carcinomas studied simultaneously on both paraffin and frozen sections, the concordance was 82% for ER and 85% for PR. In 77 breast carcinoma studied simultaneously on paraffin sections and EIA, the concordance was 74% for ER and 79.2% for PR. These results suggest that in the absence of frozen material, an immunohistochemical method on paraffin-embedded material can be used with a lower sensibility than on frozen sections. However, the detection of both ER and PR allows the identification of the hormondependent cases.
Jacquemier
J
J
Institut Paoli-Calmettes, Marseille.
Boudaoura
T
T
Martin
P M
PM
Torrente
M
M
Hassoun
J
J
D000085:Acetates; D019342:Acetic Acid; D001943:Breast Neoplasms / Q000737:chemistry*; D005260:Female; D005557:Formaldehyde; D006801:Humans; D007150:Immunohistochemistry; D016612:Paraffin Embedding; D010851:Picrates; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*; D016707:Tissue Fixation
D000085:Acetates; D010851:Picrates; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D005557:Formaldehyde; C026239:Bouin's solution; D019342:Acetic Acid
Carcinomes mammaires humains inclus en paraffine, fixés dans le liquide de Bouin. Analyse immunohistochimique des récepteurs d'estrogène et de progestérone.