Glycerol utilization in Fusarium oxysporum var. lini: regulation of transport and metabolism.
Glycerol was transported in the fungus Fusarium oxysporum var. lini by a facilitated diffusion transport system with a half-saturation constant, Ks, of 0.5 mM and a maximum velocity, Vmax, of 0.9 mmol (g dry wt)-1 h-1 at pH 5 and 25 degrees C. 1,2-Propanediol was a competitive inhibitor of glycerol transport, but the cells did not actively accumulate 1,2-propanediol. The transport system was partially constitutive. In cells grown in the presence of glucose, glycerol was not transported, indicating that the synthesis of the system was under glucose repression. Glycerol kinase and NADP(+)-dependent glycerol dehydrogenase activities were present under all physiological conditions tested. A flavin-dependent glycerol phosphate dehydrogenase was induced only when glycerol was the sole energy source in the medium. This enzyme, together with the transport system, constitute the regulated steps in the glycerol metabolic pathway.
Castro
I M
IM
Laboratory of Microbiology, Gulbenkian Institute of Science, Oeiras, Portugal.
Loureiro-Dias
M C
MC
D001693:Biological Transport, Active / Q000187:drug effects; D000431:Ethanol / Q000378:metabolism / Q000494:pharmacology; D005670:Fusarium / Q000254:growth & development / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D005990:Glycerol / Q000378:metabolism*; D005991:Glycerol Kinase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009249:NADP / Q000378:metabolism; D019946:Propylene Glycol; D011409:Propylene Glycols / Q000494:pharmacology; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism; D013696:Temperature
D011409:Propylene Glycols; D000431:Ethanol; D009249:NADP; D019946:Propylene Glycol; D013401:Sugar Alcohol Dehydrogenases; C020672:glycerol dehydrogenase; D005991:Glycerol Kinase; D005947:Glucose; D005990:Glycerol