Systemic delivery of human growth hormone by injection of genetically engineered myoblasts.
A recombinant gene encoding human growth hormone (hGH) was stably introduced into cultured myoblasts with a retroviral vector. After injection of genetically engineered myoblasts into mouse muscle, hGH could be detected in serum for 3 months. The fate of injected myoblasts was assessed by coinfecting the cells with two retroviral vectors, one encoding hGH and the other encoding beta-galactosidase from Escherichia coli. These results provide evidence that myoblasts, which can fuse into preexisting multinucleated myofibers that are vascularized and innervated, may be advantageous as vehicles for systemic delivery of recombinant proteins.
Dhawan
J
J
Department of Pharmacology, Stanford University School of Medicine, CA 94305.
Pan
L C
LC
Pavlath
G K
GK
Travis
M A
MA
Lanctot
A M
AM
Blau
H M
HM
D000818:Animals; D002454:Cell Differentiation; D002999:Clone Cells; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D013006:Growth Hormone / Q000008:administration & dosage*; D006801:Humans; D051379:Mice; D009132:Muscles / Q000166:cytology*; D011994:Recombinant Proteins / Q000008:administration & dosage*; D014162:Transfection
D011994:Recombinant Proteins; D013006:Growth Hormone