title: Aspirin-sensitive asthma: significance of the cyclooxygenase-inhibiting and protein-binding properties of analgesic drugs.
abstract: The in vitro release of endogenous and exogenous PgF2 alpha from plasma and serum proteins by aspirin and other analgesic drugs has been studied by RIA and equilibrium-dialysis techniques, respectively. Before aspirin addition, the mean plasma level of PgF2 alpha measured by RIA was significantly lower in aspirin-sensitive asthma (ASA) patients (11.3 +/- 6.5 pg/ml; n = 8) than in aspirin-tolerant asthma (ATA) patients (25.0 +/- 11.4 pg/ml; n = 21). After aspirin addition (50 micrograms/ml) the mean PgF2 alpha level detected in plasma by RIA was higher in ASA patients (97.6 +/- 5.5 pg/ml) than in ATA patients (66.9 +/- 4.5). The binding of [3H]PgF2 alpha to serum protein was significantly inhibited by NSAIDs but not by paracetamol (0.2-1.0 mM). These results implicate PgF2 alpha and the protein-binding property of analgesic drugs in the pathogenesis of aspirin-sensitive asthma.
authors.affiliation: Asthma and Allergy Unit, Sully Hospital, Cardiff, UK.
authors.forename: W R
authors.initials: WR
authors.lastname: Williams
authors.forename: A
authors.initials: A
authors.lastname: Pawlowicz
authors.forename: B H
authors.initials: BH
authors.lastname: Davies
mesh_terms: D000082:Acetaminophen / Q000494:pharmacology; D000280:Administration, Inhalation; D000328:Adult; D000368:Aged; D001241:Aspirin / Q000494:pharmacology*; D001249:Asthma / Q000188:drug therapy*; D015237:Dinoprost / Q000378:metabolism; D004361:Drug Tolerance / Q000502:physiology*; D005260:Female; D005439:Flufenamic Acid / Q000494:pharmacology; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D008528:Mefenamic Acid / Q000494:pharmacology; D008875:Middle Aged; D010653:Phenylbutazone / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000187:drug effects
chemical_list: D000082:Acetaminophen; D008528:Mefenamic Acid; D005439:Flufenamic Acid; D015237:Dinoprost; D011451:Prostaglandin-Endoperoxide Synthases; D010653:Phenylbutazone; D001241:Aspirin; D007213:Indomethacin