Angiotensin stimulates adenylate cyclase activity via prostaglandins in the adrenal glomerulosa membrane.
The effects of angiotensin II (A II) on adenylate cyclase activities in membranes of the zona glomerulosa (the capsular fraction) and the zona fasciculata (the decapsulated fraction) from rat adrenocortical glands were investigated. A time- and GTP-dependent stimulation by A II of adenylate cyclase activity was observed in the capsular fraction but not in the decapsulated fraction. The activation of adenylate cyclase by ACTH and A II was additive. Stimulation by A II was inhibited by an angiotensin antagonist, DD-3487 (DD). Moreover, the addition of a prostaglandin antagonist, a mixture of polyesters of polyphloretin phosphate (PPP) and an inhibitor of prostaglandin synthesis, indomethacin, was effective in inhibiting A II-induced stimulation of the capsular adenylate cyclase activity, suggesting that the activation of A II receptors located on the capsular membrane leads to the release of prostaglandins, which in turn stimulates the adenylate cyclase.
Shima
S
S
Department of Biochemistry, St. Marianna University School of Medicine, Kawasaki, Japan.
Fukase
H
H
D000262:Adenylyl Cyclases / Q000187:drug effects*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000527:Alprostadil / Q000037:antagonists & inhibitors / Q000502:physiology*; D000804:Angiotensin II / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000818:Animals; D006160:Guanosine Triphosphate / Q000494:pharmacology; D008566:Membranes / Q000201:enzymology; D011121:Polyphloretin Phosphate / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D015383:Zona Fasciculata / Q000201:enzymology; D015384:Zona Glomerulosa / Q000201:enzymology*
D000804:Angiotensin II; C068224:DD 3487; D006160:Guanosine Triphosphate; D000324:Adrenocorticotropic Hormone; D011121:Polyphloretin Phosphate; D000262:Adenylyl Cyclases; D000527:Alprostadil