A method for simultaneous recording of electrical activities and spectrophotometric signals from brain slice preparations in vitro.
Organ spectrophotometry has been applied to analyze cytochrome redox changes in brain slice preparations. An interface-chamber method for maintaining metabolism of brain slice tissues was devised to reduce noise on recording traces of spectrophotometric signals, and then used for continuous monitoring and simultaneous recording of electrical and optical signals from brain slices. With this method, the noise level during the recording of redox states of cytochromes was decreased to 0.0004 A unit.
Fujii
T
T
Kyoto Municipal Jr. College of Nursing, Japan.
Toita
K
K
D000818:Animals; D001921:Brain / Q000378:metabolism / Q000502:physiology*; D003580:Cytochromes / Q000378:metabolism; D004594:Electrophysiology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D013053:Spectrophotometry / Q000379:methods*
D003580:Cytochromes