title: The elastic modulus of fibrin clots and fibrinogen gels: the effect of fibronectin and dithiothreitol.
abstract: The elastic modulus (G') of factor XIIIa induced fibrinogen gels was found to be substantially lower than the G' of fibrin gels that were formed by clotting fibrinogen with thrombin. The addition of fibronectin and/or the reducing reagent dithiothreitol (DTT) to the factor XIIIa coagulation mixture led to the formation of a weaker gel structure, while the rigidity of thrombin induced clots was not appreciably affected by the inclusion of the DTT but increased somewhat in the presence of fibronectin. The reasons for the differing clot rigidities are discussed in terms of biochemical mechanisms.
authors.affiliation: New York Blood Center, New York.
authors.forename: R
authors.initials: R
authors.lastname: Procyk
authors.forename: R G
authors.initials: RG
authors.lastname: King
mesh_terms: D004229:Dithiothreitol / Q000494:pharmacology; D004548:Elasticity; D005337:Fibrin / Q000502:physiology*; D005340:Fibrinogen / Q000502:physiology*; D005353:Fibronectins / Q000494:pharmacology; D005780:Gelatin; D006801:Humans; D013917:Thrombin; D011503:Transglutaminases / Q000502:physiology
chemical_list: D005353:Fibronectins; D005780:Gelatin; D005337:Fibrin; D005340:Fibrinogen; D011503:Transglutaminases; D013917:Thrombin; D004229:Dithiothreitol