Monoclonal antibodies against Yersinia enterocolitica common antigen produced by immunization with immunoprecipitates.
Crossed immunoelectrophoresis was applied to purify Y. enterocolitica common antigen by precipitation with monospecific rabbit anti-P. aeruginosa CA antibody. Monoclonal antibodies against Y. enterocolitica CA were then raised by immunization of mice with these immunoprecipitates. This method provides the possibility of producing a panel of anti-CA monoclonal antibodies from a large number of bacterial species.
Bagger
Y Z
YZ
Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007123:Immunoelectrophoresis, Two-Dimensional; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D015008:Yersinia enterocolitica / Q000276:immunology*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; C017286:enterobacterial common antigen