Apolipoprotein B is a major perforin inhibitor protein in human serum.
We purified the high-molecular-weight perforin inhibitor protein from normal human serum using DEAE-cellulose, HPLC-gel filtration and hydroxylapatite chromatography. This protein was shown to be identical to the serum apolipoprotein B-100 in terms of amino acid composition and the sequence of the digested peptides. This inhibitor protein not only inhibits the membrane binding activity of perforin but also the pore insertion activity of membrane-bound perforin.
Matsuda
K
K
National Institute of Neuroscience, NCNP, Tokyo, Japan.
Ishiura
S
S
Koizumi
H
H
Kawasaki
H
H
Arahata
K
K
Sugita
H
H
D000595:Amino Acid Sequence; D001055:Apolipoproteins B / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D015153:Blotting, Western; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D006801:Humans; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000037:antagonists & inhibitors*; D008969:Molecular Sequence Data; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D012689:Sequence Homology, Nucleic Acid; D013602:T-Lymphocytes, Cytotoxic
D001055:Apolipoproteins B; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D052899:Pore Forming Cytotoxic Proteins; D054353:Perforin