Temperature-sensitive variants of Histoplasma capsulatum isolated from patients with acquired immunodeficiency syndrome.
Histoplasma capsulatum isolates from three St. Louis area AIDS patients with disseminated histoplasmosis were found to be closely related to the temperature-sensitive, previously unique, Downs strain based on growth phenotype and restriction fragment length polymorphisms (RFLP) involving mitochondrial DNA, ribosomal DNA, and the yps-3 gene. H. capsulatum isolates from five non-AIDS patients in the St. Louis area with disseminated histoplasmosis or chronic pulmonary histoplasmosis had the growth phenotype and RFLP pattern characteristic of most strains isolated from other regions of the USA.
Spitzer
E D
ED
Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.
Keath
E J
EJ
Travis
S J
SJ
Painter
A A
AA
Kobayashi
G S
GS
Medoff
G
G
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D000328:Adult; D004271:DNA, Fungal / Q000032:analysis; D004272:DNA, Mitochondrial / Q000032:analysis; D006658:Histoplasma / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity; D006660:Histoplasmosis / Q000150:complications / Q000382:microbiology*; D006801:Humans; D008923:Missouri; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D013696:Temperature; D014774:Virulence
D004271:DNA, Fungal; D004272:DNA, Mitochondrial