Uremic serum contains humoral factor(s) larger than fifty kilodaltons which suppresses endothelin production in cultured endothelial cells.
Direct effects of human uremic serum on the production of endothelin-1 in cultured porcine endothelial cells were examined in this study. Uremic serum decreased the level of monomeric endothelin-1 secreted into the culture medium by endothelial cells. This effect occurred at a transcriptional step because uremic serum decreased the endothelin-1 mRNA level in those cells. For the partial characterization of this inhibitory activity, uremic serum was fractionated with a centricut column. Uremic serum contains humoral factor(s) larger than 50 kD which suppress the endothelin-1 mRNA level in cultured endothelial cells.
Koyama
H
H
Second Department of Internal Medicine, Osaka City University Medical School, Japan.
Tahara
H
H
Shoji
T
T
Nishizawa
Y
Y
Inaba
M
M
Otani
S
S
Yanagisawa
M
M
Ishiguro
Y
Y
Takanashi
N
N
Morii
H
H
D000818:Animals; D002478:Cells, Cultured; D016232:Endothelins / Q000096:biosynthesis* / Q000235:genetics; D004730:Endothelium, Vascular / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D013552:Swine; D014511:Uremia / Q000097:blood*
D016232:Endothelins; D012333:RNA, Messenger