Development of bacillus Calmette-Guérin-induced antitumor activity in peripheral blood mononuclear cells.
Peripheral blood mononuclear cells (PBMNCs) after bladder instillation of bacillus Calmette-Guérin (BCG) to patients with superficial bladder carcinoma showed lytic activity against natural killer (NK)-sensitive and NK-resistant target tumor cells. PBMNCs in normal volunteers were isolated and cultured with BCG (5 micrograms/ml) in vitro. Maximum cytotoxicity was obtained within 24 h. Cytosine arabinoside as a DNA synthesis inhibitor did not inhibit this killer cell activation. Monoclonal antibody treatment revealed that both precursor and effector cells were NK cell type. However, effector cells had a wider spectrum of target cells than usual NK cells. The recognition specificity from cold target competition experiments was selective.
Koga
S
S
Department of Urology, Nagasaki University School of Medicine, Japan.
Taniguchi
K
K
Nishikido
M
M
Kubota
S
S
Yamashita
S
S
Kanetake
H
H
Saito
Y
Y
D000283:Administration, Intravesical; D001500:BCG Vaccine / Q000276:immunology / Q000627:therapeutic use*; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D001749:Urinary Bladder Neoplasms / Q000276:immunology / Q000628:therapy*
D001500:BCG Vaccine