In vitro enzymatic synthesis of new penicillins containing keto acids as side chains.
Seven different penicillins containing alpha-ketobutyric, beta-ketobutyric, gamma-ketovaleric, alpha-ketohexanoic, delta-ketohexanoic, epsilon-ketoheptanoic, and alpha-ketooctanoic acids as side chains have been synthesized in vitro by incubating the enzymes phenylacetyl coenzyme A (CoA) ligase from Pseudomonas putida and acyl-CoA:6-aminopenicillanic acid acyltransferase from Penicillium chrysogenum with CoA, ATP, Mg(2+), dithiothreitol, 6-aminopenicillanic acid, and the corresponding side chain precursor.
Ferrero
M A
MA
Departamento Interfacultativo de Bioquímica y Biología Molecular, Facultades de Veterinaria y Biología, Universidad de León, Spain.
Reglero
A
A
Martínez-Blanco
H
H
Fernández-Valverde
M
M
Luengo
J M
JM
D007651:Keto Acids / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D016982:Micrococcus luteus / Q000187:drug effects; D010406:Penicillins / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D016958:Pseudomonas putida / Q000378:metabolism; D013329:Structure-Activity Relationship
D007651:Keto Acids; D010406:Penicillins