A combined physical and genetic map of Pseudomonas aeruginosa PAO.
A combined physical and genetic map of Pseudomonas aeruginosa PAO was constructed by pulsed-field gel electrophoresis and Southern hybridization using cosmid clones from a genomic library carrying known genes. A total of 37 SpeI restriction fragments have been mapped on the 5862 kb genome, and fragment contiguity demonstrated by hybridization with clones from a SpeI junction fragment library and fragments obtained by partial SpeI digestion, both derived from the P. aeruginosa PAO chromosome.
Ratnaningsih
E
E
Department of Genetics and Developmental Biology, Monash University, Clayton, Victoria, Australia.
Dharmsthiti
S
S
Krishnapillai
V
V
Morgan
A
A
Sinclair
M
M
Holloway
B W
BW
D015139:Blotting, Southern; D003001:Cloning, Molecular; D003360:Cosmids; D004251:DNA Transposable Elements; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D015698:Genomic Library; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D015183:Restriction Mapping
D004251:DNA Transposable Elements; C056436:endodeoxyribonuclease SpeI; D015252:Deoxyribonucleases, Type II Site-Specific