Characteristics of various synthetic peptide calpain inhibitors and their application for the analysis of platelet reaction.
Calpeptin (a cell permeable synthetic peptide calpain inhibitor) inhibited the generation of thromboxane B2 (TxB2) by the direct inhibition on Tx synthetase in platelets at the concentrations more than 30 microM. Calpeptin, its analogues and E-64d (EST) were further examined with regard to cell permiability and inhibitory spectra. Among all compounds, only calpeptin inhibited the degradation of substrate proteins of calpain with negligible effect on TxB2 generation in intact platelets at the concentrations less than 30 microM. These concentrations of calpeptin did not inhibit the platelet aggregation, the elevation of [Ca2+], nor the formation of inositol 1,4,5-trisphosphate (IP3) in thrombin or collagen activated platelets. These results indicate that calpain dose not participate in the process of platelet activation induced by thrombin or collagen.
Ariyoshi
H
H
Department of Surgery II, Osaka University Medical School, Japan.
Shiba
E
E
Kambayashi
J
J
Sakon
M
M
Tsujinaka
T
T
Uemura
Y
Y
Mori
T
T
D001095:Arachidonic Acids / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002154:Calpain / Q000037:antagonists & inhibitors / Q000502:physiology*; D003094:Collagen / Q000502:physiology; D016861:Cyclooxygenase Inhibitors; D004151:Dipeptides / Q000494:pharmacology*; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D015394:Molecular Structure; D015539:Platelet Activation / Q000502:physiology*; D013329:Structure-Activity Relationship; D013917:Thrombin / Q000378:metabolism; D013929:Thromboxane B2 / Q000378:metabolism; D013930:Thromboxane-A Synthase / Q000037:antagonists & inhibitors
D001095:Arachidonic Acids; D016861:Cyclooxygenase Inhibitors; D004151:Dipeptides; C071482:calpeptin; D013929:Thromboxane B2; D015544:Inositol 1,4,5-Trisphosphate; D003094:Collagen; D013917:Thrombin; D002154:Calpain; D013930:Thromboxane-A Synthase; D002118:Calcium