Effect of human urinary colony-stimulating factor on experimental Legionella pneumophila infection in guinea pigs.
The effect of human urinary colony-stimulating factor (CSF-HU) on in-vitro phagocytosis of Legionella pneumophila, superoxide anion production and intracellular killing of Legion, pneumophila by guinea pig alveolar macrophages was studied. Alveolar macrophages when incubated with CSF-HU demonstrated enhanced phagocytic activity and superoxide production. The bactericidal activity of treated cells was enhanced when they were afterwards incubated with a low concentration of bacteria, but these cells failed to inhibit bacterial multiplication when they were incubated with bacteria in a 1:10 ratio. In vivo, an increase in the total peripheral leucocyte count in guinea pigs after intraperitoneal injection of CSF-HU occurred and this was accompanied by an increase in granulocyte and monocyte counts. A comparison of treatment of experimental Legion, pneumophila pneumonia with CSF-HU alone, ceftazidime alone and a combination revealed lower mortality rates in the group receiving combination therapy. These data suggest a possible function for CSF-HU of enhancing antibiotic therapy in Legionnaires' disease.
Owan
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I
First Department of Internal Medicine, Faculty of Medicine, University of the Ryukus, Okinawa, Japan.
Saito
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A
D000818:Animals; D001770:Blood Bactericidal Activity / Q000187:drug effects; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D003115:Colony-Stimulating Factors / Q000494:pharmacology / Q000627:therapeutic use*; D006168:Guinea Pigs; D007875:Legionella / Q000187:drug effects; D007877:Legionnaires' Disease / Q000188:drug therapy* / Q000382:microbiology; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects; D011650:Pulmonary Alveoli / Q000166:cytology; D013481:Superoxides / Q000378:metabolism
D003115:Colony-Stimulating Factors; D013481:Superoxides