[An electron microscopic study of the localization of the activity of the ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase component of the Na, K-ATPase complex in the rat brain].
The localization of an ouabain-sensitive potassium-dependent p-nitrophenylphosphatase part of the Na+,K(+)-ATPase complex in the white rat's brain has been studied at the ultrastructural level. The physiological pH of incubation medium highly increases the specificity of ultracytochemical enzyme demonstration. The main characteristics of the enzymatic p-NPP hydrolysis used for this methodological technique are discussed.
Zinchuk
V S
VS
Kiseleva
A F
AF
Tushevskiĭ
V F
VF
D009597:4-Nitrophenylphosphatase / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D008297:Male; D008854:Microscopy, Electron; D009413:Nerve Fibers, Myelinated / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D010042:Ouabain / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects / Q000378:metabolism*
D010042:Ouabain; D009597:4-Nitrophenylphosphatase; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium
Elektronno-mikroskopicheskoe izuchenie lokalizatsii aktivnosti uabain-chuvstvitel'noĭ, kaliĭzavisimoĭ p-nitrofenilfosfataznoĭ sostavliaiushcheĭ Na, K-ATFaznogo kompleksa golovnogo mozga krysy.