title: Purification of lysyl oxidase from piglet skin by selective interaction with Sephacryl S-200.
abstract: A method is described for the purification of piglet skin lysyl oxidase from a crude urea extract by two simple chromatographic procedures. The method relies on the selective interaction between Sephacryl S-200 and lysyl oxidase that occurs in the absence of urea and in conditions of low ionic strength.
authors.affiliation: Department of Biochemistry, University of Edinburgh Medical School, Scotland, U.K.
authors.forename: D R
authors.initials: DR
authors.lastname: Shackleton
authors.forename: D J
authors.initials: DJ
authors.lastname: Hulmes
mesh_terms: D000180:Acrylic Resins; D000594:Amino Acid Oxidoreductases / Q000302:isolation & purification*; D000818:Animals; D002846:Chromatography, Affinity; D008249:Protein-Lysine 6-Oxidase / Q000302:isolation & purification*; D012867:Skin / Q000201:enzymology*; D013552:Swine
chemical_list: D000180:Acrylic Resins; C023049:sephacryl S 200; D000594:Amino Acid Oxidoreductases; D008249:Protein-Lysine 6-Oxidase