The regulatory role of external cations on human polymorphonuclear phagocyte chemiluminescence.
The effect of the external cations Na+ and Ca2+ on polymorphonuclear chemiluminescence was investigated. Both Ca2+ in the range of 0.2-2 mM and Na+ in the range of 114-143 mM showed a dose dependent increase in polymorphonuclear chemiluminescence, irrespective of the concurrent increase in osmolality. The Na+/H+ antiport inhibitor Amiloride decreased the response significantly. These effects were observed using buffers commonly used for chemiluminescence studies and indicate the importance of defining the Ca2+ and Na+ composition of the buffers used in chemiluminescence assays.
Janah
S
S
Department of Chemical Pathology, Charing Cross and Westminster Medical School, London, U.K.
Mali
B
B
Fleck
A
A
D000584:Amiloride / Q000494:pharmacology; D002021:Buffers; D002118:Calcium / Q000494:pharmacology*; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D008163:Luminescent Measurements; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D009994:Osmolar Concentration; D015398:Signal Transduction / Q000187:drug effects; D012964:Sodium / Q000494:pharmacology*; D017923:Sodium-Hydrogen Exchangers
D002021:Buffers; D002352:Carrier Proteins; D017923:Sodium-Hydrogen Exchangers; D009240:N-Formylmethionine Leucyl-Phenylalanine; D000584:Amiloride; D012964:Sodium; D002118:Calcium