The effect of unsaturated fatty acids on sodium nitroprusside stimulation of guanylate cyclase in the human astrocytoma clone, D384, and the human neuroblastoma clone, NB1-G.
Sodium nitroprusside (SNP) stimulates cGMP formation to a greater extent in 20,000 g supernatant fractions of the human neuroblastoma clones NB1-G and SH-SY5Y than in the human astrocytoma clone D384. This suggests that these cell lines contain the soluble form of guanylate cyclase. Arachidonic, 8,11,14- and 11,14,17-eicosatrienoic acids inhibit SNP (10(-4) M)-stimulated cGMP formation more potently than the C18 unsaturated fatty acids linolenic and linoleic acids in D384 and NB1-G. In contrast the C20 saturated fatty acid, arachidic acid had little effect even at 10(-4) M concentration. In addition arachidonic and 8,11,14-eicosatrienoic acids inhibited basal guanylate cyclase activity, in NB1-G, over the same concentration range as they inhibited SNP-stimulated cGMP formation. No evidence could be obtained for the stimulation of guanylate cyclase by arachidonic acid in either NB1-G or D384. These results provide further support for suggestions that arachidonic acid or its metabolites may be important regulators of cGMP formation in the nervous system.
Vaughan
P F
PF
Department of Cardiovascular Studies, University of Leeds, U.K.
Ball
S G
SG
D015126:8,11,14-Eicosatrienoic Acid / Q000494:pharmacology; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D001254:Astrocytoma / Q000201:enzymology*; D006152:Cyclic GMP / Q000096:biosynthesis; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005292:Ferricyanides / Q000494:pharmacology*; D006162:Guanylate Cyclase / Q000378:metabolism*; D006801:Humans; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D008042:Linolenic Acids / Q000494:pharmacology; D009447:Neuroblastoma / Q000201:enzymology*; D009599:Nitroprusside / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D001095:Arachidonic Acids; D005231:Fatty Acids, Unsaturated; D005292:Ferricyanides; D008041:Linoleic Acids; D008042:Linolenic Acids; D009599:Nitroprusside; C034152:11,14,17-eicosatrienoic acid; D016718:Arachidonic Acid; D019787:Linoleic Acid; D006162:Guanylate Cyclase; D015126:8,11,14-Eicosatrienoic Acid; D006152:Cyclic GMP