title: Phenotypic variation amongst genotypically homogeneous Legionella pneumophila serogroup 1 isolates: implications for the investigation of outbreaks of Legionnaires' disease.
abstract: One hundred and seventy-nine isolates of Legionella pneumophila serogroup 1, obtained from a site associated with an outbreak of Legionnaires' disease, were examined by monoclonal antibody subgrouping, restriction fragment length polymorphism typing, restriction endonuclease analysis and plasmid content. Nine distinct phenotypes were detected but at the genotypic level all strains were closely related. The data presented indicate that phenotypic variation of a single parent strain can occur within an environmental site. The implications of these findings are discussed in relation to the investigation of outbreaks of Legionnaires' disease.
authors.affiliation: PHLS Legionella Reference Unit, Central Public Health Laboratory, Colindale, London, UK.
authors.forename: T G
authors.initials: TG
authors.lastname: Harrison
authors.forename: N A
authors.initials: NA
authors.lastname: Saunders
authors.forename: A
authors.initials: A
authors.lastname: Haththotuwa
authors.forename: G
authors.initials: G
authors.lastname: Hallas
authors.forename: R J
authors.initials: RJ
authors.lastname: Birtles
authors.forename: A G
authors.initials: AG
authors.lastname: Taylor
mesh_terms: D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks; D004739:England / Q000453:epidemiology; D005838:Genotype; D006801:Humans; D007875:Legionella / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D007877:Legionnaires' Disease / Q000453:epidemiology / Q000382:microbiology*; D010641:Phenotype; D010957:Plasmids; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D012703:Serotyping; D014871:Water Microbiology
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial