title: Sequences homologous to glutamic acid decarboxylase cDNA are present on mouse chromosomes 2 and 10.
abstract: The chromosomal locations of mouse DNA sequences homologous to a feline cDNA clone encoding glutamic acid decarboxylase (GAD) were determined. Although cats and humans are thought to have only one gene for GAD, GAD cDNA sequences hybridize to two distinct chromosomal loci in the mouse, chromosomes 2 and 10. The chromosomal assignment of sequences homologous to GAD cDNA was determined by Southern hybridization analysis using DNA from mouse-hamster hybrid cells. Mouse genomic sequences homologous to GAD cDNA were isolated and used to determine that GAD is encoded by a locus on mouse chromosome 2 (Gad-1) and that an apparent pseudogene locus is on chromosome 10 (Gad-1ps). An interspecific backcross and recombinant inbred strain sets were used to map these two loci relative to other loci on their respective chromosomes. The Gad-1 locus is part of a conserved homology between mouse chromosome 2 and the long arm of human chromosome 2.
authors.affiliation: Jackson Laboratory, Bar Harbor, Maine 04609.
authors.forename: M H
authors.initials: MH
authors.lastname: Brilliant
authors.forename: G
authors.initials: G
authors.lastname: Szabo
authors.forename: Z
authors.initials: Z
authors.lastname: Katarova
authors.forename: C A
authors.initials: CA
authors.lastname: Kozak
authors.forename: T M
authors.initials: TM
authors.lastname: Glaser
authors.forename: R J
authors.initials: RJ
authors.lastname: Greenspan
authors.forename: D E
authors.initials: DE
authors.lastname: Housman
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004587:Electrophoresis, Agar Gel; D005968:Glutamate Decarboxylase / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D051379:Mice / Q000235:genetics*; D008810:Mice, Inbred C57BL; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D005968:Glutamate Decarboxylase