Effectiveness of taurine in protecting biomembrane against oxidant.
The effect of taurine in protecting biomembrane attacked by hypochlorous acid (HOCl) was examined using canine erythrocytes which had been pre-treated with HOCl. In the treatment, most of the HOCl was consumed as a result of its reaction with a number of electrophilic substances, such as free amino groups (-NH2) in the membrane, whereas hemoglobin inside the cells was not oxidized. The lysis of HOCl-treated erythrocytes was dependent on the concentration of HOCl and on the incubation time at 37 degrees C. Taurine inhibited the lysis at 37 degrees C in a dose dependent manner. During the incubation of HOCl-treated erythrocytes with taurine, an appreciable amount of monochlorotaurine (TauNHCl) was detected in the supernate. This suggests that taurine might remove the oxidized chlorine from HOCl-treated erythrocytes, resulting in the production of TauNHCl. The effect of taurine on the removal of Cl+ moiety was further examined using Sepharose gel with free amino groups. Taurine removed Cl+ moiety from HOCl-treated Sepharose gel, and the yield of TauNHCl depended on the concentration of taurine and the incubation time. These results indicate that taurine might inhibit the hemolysis by scavenging the oxidized chlorine moiety from the HOCl-treated erythrocytes. Inhibition of the HOCl-induced hemolysis was also observed with other amino acids.(ABSTRACT TRUNCATED AT 250 WORDS)
Nakamori
K
K
Research Center, Taisho Pharmaceutical Co., Ltd., Saitama, Japan.
Koyama
I
I
Nakamura
T
T
Yoshida
T
T
Umeda
M
M
Inoue
K
K
D000818:Animals; D004285:Dogs; D004910:Erythrocyte Membrane / Q000502:physiology*; D006997:Hypochlorous Acid / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D013654:Taurine / Q000502:physiology*
D013654:Taurine; D006997:Hypochlorous Acid