title: Specific DNA fragments coding for ST1 and LT1 toxins, and K88 (F4) adhesin in enterotoxigenic Escherichia coli.
abstract: Ten porcine strains of enterotoxigenic Escherichia coli possessing the K88 (F4) adhesion fimbriae, were selected for study of enterotoxin- and fimbriae-encoding plasmids. Plasmid DNA, separated according to size by gel electrophoresis was transferred to nylon membranes by Southern blotting, and hybridized with enzyme-labelled oligonucleotide probes for ST1 and LT1 enterotoxins, and a 32P-labelled probe for the F4 fimbriae. Plasmids possessing the enterotoxin genes ranged from 50 MDa to 78 MDa in size. The ST1 genes were located on a common 8-MDa EcoR1 restriction endonuclease fragment, while the LT1 genes were located on a 4.5-MDa EcoR1 fragment from the different plasmids. Plasmids with the F4 genes ranged from 50 MDa to 118 MDa in size, but the F4 encoding genes were located on a common 3-MDa HindIII restriction endonuclease fragment. ST1 and LT1 genes were found on the same plasmid in only one strain, LT1 and F4 genes on the same plasmids in 5 strains, while no plasmid contained genes for both ST1 and F4.
authors.affiliation: Department of Microbiology and Immunology, Norwegian College of Veterinary Medicine, Oslo.
authors.forename: Y
authors.initials: Y
authors.lastname: Wasteson
authors.forename: O
authors.initials: O
authors.lastname: Olsvik
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics; D000954:Antigens, Surface / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D004768:Enterotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D010957:Plasmids; D013552:Swine / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C026888:K88 antigen, E coli; C034151:heat stable toxin (E coli); D036781:Fimbriae Proteins; C027538:heat-labile enterotoxin, E coli