title: The binding of colonization factor antigens of enterotoxigenic Escherichia coli to intestinal cell membrane proteins.
abstract: We examined the binding of colonization factor antigens (CFAs) of enterotoxigenic Escherichia coli to electrophoretically separated membrane components of rabbit intestinal brush borders or human intestinal (and non-intestinal) cell lines using an immunoblotting technique. Both CFA/I and CFA/II bound to distinct membrane components which seemed to be identical in rabbit brush borders and in a human intestinal cell line; these binding structures were mainly missing in membranes from epithelial cell lines of non-intestinal origin. Both shared and specific binding components were identified for CFA/I and the different subcomponents of CFA/II (CS1, CS2 and CS3), respectively. Chloroform-methanol extraction of lipids from the cell membranes did not change the binding pattern for either CFA/I or CFA/II suggesting that the binding occurred to (glyco)proteins rather than to (glyco)lipids.
authors.affiliation: Department of Medical Microbiology, University of Göteborg, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Wennerås
authors.forename: J
authors.initials: J
authors.lastname: Holmgren
authors.forename: A M
authors.initials: AM
authors.lastname: Svennerholm
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000276:immunology / Q000378:metabolism; D001427:Bacterial Toxins / Q000096:biosynthesis; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism / Q000382:microbiology; D004768:Enterotoxins / Q000096:biosynthesis; D004926:Escherichia coli / Q000276:immunology / Q000378:metabolism*; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000276:immunology / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007422:Intestines / Q000382:microbiology*; D008565:Membrane Proteins / Q000378:metabolism; D011817:Rabbits
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; D008565:Membrane Proteins; C022924:colonization factor antigens; D036781:Fimbriae Proteins