Human Alu subfamilies and their methylation revealed by blot hybridization.
By a simple direct blot hybridization strategy, the existence of human Alu family subfamilies is confirmed. Using consensus restriction cleavage sites, individual bands can be resolved from genomic human DNA digests corresponding to three distinct Alu subfamilies. Digestion with methylation sensitive and insensitive restriction enzymes shows that the numerous CpG residues in the youngest Alu subfamilies are largely methylated in vivo, suggesting a model for the transcriptional regulation of Alu repeats.
Schmid
C W
CW
Department of Genetics, University of California, Davis 95616.
D001483:Base Sequence; D016384:Consensus Sequence; D004247:DNA / Q000378:metabolism*; D015226:Dinucleoside Phosphates / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008745:Methylation; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D004247:DNA; D015226:Dinucleoside Phosphates; C015772:CpG dinucleotide