title: Oxodiene formation during the Vicia sativa lipoxygenase-catalyzed reaction: occurrence of dioxygenase and fatty acid lyase activities associated in a single protein.
abstract: An enzyme with at least dual activities, lipoxygenase and fatty acid lyase, has been isolated from Vicia sativa seeds. The enzyme utilizes directly linoleic acid as substrate. The enzyme had a pH optimum at 5.8 for the two activities and converted linoleic acid into two products: 9-hydroperoxylinoleic acid and trans-2, cis-4 decadienal. The enzyme does not act on 13- or 9- fatty acid hydroperoxide isomers. An enzymatic reaction for the biogenesis of trans-2, cis-4- decadienal is proposed. This involves the synthesis of an intermediate peroxyl radical due to oxygen insertion in carbon 9 of linoleic acid. This intermediate peroxyl radical may be converted into 9-HPOD and 2,4-decadienal.
authors.affiliation: C.J.F. INSERM 88-03, Faculté de Médecine, Limoges, France.
authors.forename: R H
authors.initials: RH
authors.lastname: Andrianarison
authors.forename: H
authors.initials: H
authors.lastname: Rabinovitch-Chable
authors.forename: J L
authors.initials: JL
authors.lastname: Beneytout
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D008084:Lipoxygenase / Q000302:isolation & purification / Q000378:metabolism*; D008190:Lyases / Q000378:metabolism*; D008970:Molecular Weight; D010105:Oxygenases / Q000378:metabolism*; D010944:Plants / Q000201:enzymology*
chemical_list: D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D019787:Linoleic Acid; D010105:Oxygenases; D008084:Lipoxygenase; D008190:Lyases