Mutagenic activity of 6-azido deoxyhexoses and azido alcohols in Salmonella typhimurium and its inhibition by a structure-similar carbon source in the medium.
6-Azido-6-deoxy (AZd) derivatives of D-glucose, D-mannose, D-altrose, D-allose, L-idose, D-galactose, D-galactonic acid and D-galactitol, 3-azido-1,2-propanediol (azidoglycerol), 3,1-diazido-2-propanol (diazidoglycerol) and (at much higher doses) 2-azidoethanol were mutagenic in Salmonella typhimurium strains TA100 and TA1535. The mutagenic response was similar to that induced by sodium azide, i.e., the azido compounds failed to induce mutations in strain TA98, and mutagenesis was independent of plasmid pKM101, and independent of external activation. The specific mutagenicity (his+ rev/mmole) of AZd-glucose and AZd-galactose was decreased with increasing concentrations of D-glucose or D-galactose in the minimal agar medium and enhanced 100-fold or more when 0.2% citrate rather than 0.2% glucose served as the carbon source in the medium. Similarly, the mutagenic efficiency of azidoglycerol was inhibited by glycerol but not by D-glucose or D-galactose; however, the mutagenicity of sodium azide was not influenced by any of these carbon sources in the medium. The inhibition of the mutagenic action of azido hexoses and azido alcohols by non-azido structural analogs is assumed to reside in competition in transmembrane transport or for the metabolic pathways.
Juricek
M
M
Institute of Experimental Botany, Czechoslovak Academy of Sciences, Prague.
Gruz
P
P
Veleminsky
J
J
Stanek
J
J
Kefurt
K
K
Moravcova
J
J
Jary
J
J
D000438:Alcohols / Q000037:antagonists & inhibitors / Q000633:toxicity; D001386:Azides / Q000037:antagonists & inhibitors / Q000633:toxicity*; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006601:Hexoses / Q000037:antagonists & inhibitors / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity*; D011409:Propylene Glycols / Q000633:toxicity; D012486:Salmonella typhimurium / Q000187:drug effects; D019810:Sodium Azide; D013329:Structure-Activity Relationship
D000438:Alcohols; D001386:Azides; D003470:Culture Media; D006601:Hexoses; D009153:Mutagens; D011409:Propylene Glycols; C052086:azidoglycerol; D002244:Carbon; D019810:Sodium Azide; D005947:Glucose