[Effects of somatostatin on human thyroid folliculi in vitro].
Somatostatin (SRIF) inhibits calcitonin and T3-T4 secretion in thyroid. We have investigated the in vitro effect of SRIF on the basal and TSH induced [3H]thy incorporation, thyroglobulin (tgb) RNA and cAMP level in follicular cells, isolated from normal and adenomatous human thyroids. [3H]thy uptake has been evaluated as TCA-precipitable material in 2, 4, 8, 24 h incubated follicles and 24 h incubated adherent cells. Tgb RNA has been quantified with cytoplasmic dot blot hybridization and cAMP level with RIA method. SRIF reduces basal and TSH-induced [3H]thy in both suspension follicles and epithelial adherent cells. However it does not modify tgb RNA nor cAMP levels in incubated follicles. These data suggest a direct antiproliferative effect of SRIF on human thyroid.
Hanau
S
S
Centro di Studi Biochimici delle Patologie del Genoma Umano, Università.
Rossi
R
R
Piva
R
R
Maestri
I
I
Margutti
A
A
Trasforini
G
G
Pansini
G
G
Degli Uberti
E
E
del Senno
L
L
D006801:Humans; D066298:In Vitro Techniques; D013004:Somatostatin / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism*
D013004:Somatostatin; D013936:Thymidine
Effetti della somatostatina su follicoli tiroidei umani in vitro.