The mouse episialin (Muc1) gene and its promoter: rapid evolution of the repetitive domain in the protein.
We have cloned the Muc1 gene of the mouse, encoding the murine equivalent of human episialin (also known as EMA or PEM), a mucin-like glycoprotein that is overexpressed in carcinoma cells. The extracellular domain of the mouse protein, that mainly consists of tandem repeats, contains 16 repeats of variable length and sequence, whereas the human protein usually contains between 30 and 90 nearly identical repeats. The murine repeats contain more potential O-glycan side chains and this may result in a more extended conformation of the murine protein. The transmembrane and cytoplasmic domains of the protein show about 90% conservation. The promoter region shows many conserved regions that could function as transcription factor binding sites.
Vos
H L
HL
Division of Tumor Biology, Netherlands Cancer Institute, Amsterdam.
de Vries
Y
Y
Hilkens
J
J
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000235:genetics; D001483:Base Sequence; D005075:Biological Evolution; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D018396:Mucin-1; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D013154:Spleen / Q000502:physiology
D000941:Antigens; D008562:Membrane Glycoproteins; D018396:Mucin-1; D009838:Oligodeoxyribonucleotides; D004247:DNA