title: Ubiquitin: preparative chemical synthesis, purification and characterization.
abstract: An improved total synthesis of ubiquitin has been achieved by the Fmoc/t-butyl solid-phase methodology using NGPmc protection of the Arg residues. Optimization of the purification protocol has offered the material in substantial quantity (90 mg) in an overall yield of 4.2%. Characterization of the isolated products has opened the way to the studies of the folding pathway and the preparation of structural analogues.
authors.affiliation: Department of Chemistry, University of Edinburgh, U.K.
authors.forename: O
authors.initials: O
authors.lastname: Ogunjobi
authors.forename: R
authors.initials: R
authors.lastname: Ramage
mesh_terms: D002851:Chromatography, High Pressure Liquid; D007525:Isoelectric Focusing; D008722:Methods; D011487:Protein Conformation; D014452:Ubiquitins / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D014452:Ubiquitins