title: Use of 33P for Sanger DNA sequencing.
abstract: The use of [alpha-33P]deoxyadenosine 5'-triphosphate ([alpha-33P]dATP) in DNA sequencing has been described. 33P has a maximum beta-emission energy that is 50% stronger than 35S, but fivefold weaker than 32P. As a result, sequences generated using [alpha-33P]dATP have short exposure times like 32P, yet they maintain band resolution similar to 35S. Handling of [alpha-33P]dATP is straightforward because no special lead or Plexiglas shielding is necessary.
authors.affiliation: Du Pont Merck Pharmaceutical Company, Research and Development, Wilmington, DE 19880-0328.
authors.forename: R J
authors.initials: RJ
authors.lastname: Zagursky
authors.forename: P S
authors.initials: PS
authors.lastname: Conway
authors.forename: M A
authors.initials: MA
authors.lastname: Kashdan
mesh_terms: D001483:Base Sequence; D004247:DNA; D003838:Deoxyadenine Nucleotides; D005821:Genetic Techniques; D010761:Phosphorus Radioisotopes
chemical_list: D003838:Deoxyadenine Nucleotides; D010761:Phosphorus Radioisotopes; D004247:DNA; C026600:2'-deoxyadenosine triphosphate