title: A human hepatocellular carcinoma cell line with multiple copies of structurally normal chromosomes.
abstract: At Veterans General Hospital (VGH), a cell line, HA59T(HA59T/VGH), was established from a primary hepatocellular carcinoma of a 52-year-old Chinese male patient. G-banded metaphases were analyzed at passages 12 and 62. Of the 200 cells counted, 68% had 100-110 chromosomes/cell at passage 12, and 74% had 90-100 chromosomes/cell at passage 62. The presence of multiple copies of a structurally normal chromosome in a single cell was common for most of the chromosomes. Both X and Y sex chromosomes were normally present in the majority of the cells. This cell line is karyotypically different from those reported in other literature.
authors.affiliation: Institute of Genetics, National Yang Ming Medical College, Taipei, Taiwan, R.O.C.
authors.forename: K D
authors.initials: KD
authors.lastname: Wuu
authors.forename: S W
authors.initials: SW
authors.lastname: Wuu
authors.forename: C P
authors.initials: CP
authors.lastname: Hu
authors.forename: C M
authors.initials: CM
authors.lastname: Chang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002869:Chromosome Aberrations; D006801:Humans; D007621:Karyotyping; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured