title: The NH2-terminal extension of rat liver arginyl-tRNA synthetase is responsible for its hydrophobic properties.
abstract: Rat liver arginyl-tRNA synthetase is found in extracts either as a component (Mr = 72,000) of the multienzyme aminoacyl-tRNA synthetase complex or as a low molecular weight (Mr = 60,000) free protein. The two forms are thought to be identical except for an extra peptide extension at the NH2-terminus of the larger form which is required for its association with the complex, but is unessential for catalytic activity. It has been suggested that interactions among synthetases in the multienzyme complex are mediated by hydrophobic domains on these peptide extensions of the individual proteins. To test this model we have purified to homogeneity the larger form of arginyl-tRNA synthetase and compared its hydrophobicity to that of its low molecular weight counterpart. We show that whereas the smaller protein displays no hydrophobic character, the larger protein demonstrates a high degree of hydrophobicity. No lipid modification was found on the high molecular weight protein indicating that the amino acid sequence itself is responsible for its hydrophobic properties. These findings support the proposed model for synthetase association within the multienzyme complex.
authors.affiliation: Department of Biochemistry, University of Connecticut Health Center, Farmington 06030.
authors.forename: S
authors.initials: S
authors.lastname: Huang
authors.forename: M P
authors.initials: MP
authors.lastname: Deutscher
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001126:Arginine-tRNA Ligase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000302:isolation & purification; D011487:Protein Conformation; D051381:Rats
chemical_list: D005227:Fatty Acids; D009097:Multienzyme Complexes; D001126:Arginine-tRNA Ligase