title: Characterization of complementary DNA encoding the precursor for gonadotropin-releasing hormone and its associated peptide from a teleost fish.
abstract: Reproductive maturity among male African cichlids Haplochromis burtoni is cued by a series of environmental and social interactions and is mediated physiologically by GnRH. A cDNA clone encoding the precursor for GnRH was isolated from this teleost. The molecular architecture of the predicted prohormone is analogous to that of the previously characterized mammalian forms; however, the predicted sequence of the associated peptide is strikingly different. Attempts to isolate a putative second precursor using low stringency hybridization were not successful despite evidence that a second related decapeptide exists in at least some teleost species.
authors.affiliation: Vollum Institute, Oregon Health Sciences University, Portland 97201.
authors.forename: C T
authors.initials: CT
authors.lastname: Bond
authors.forename: R C
authors.initials: RC
authors.lastname: Francis
authors.forename: R D
authors.initials: RD
authors.lastname: Fernald
authors.forename: J P
authors.initials: JP
authors.lastname: Adelman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010471:Perches / Q000235:genetics*; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D011498:Protein Precursors; D007987:Gonadotropin-Releasing Hormone; D004247:DNA