Phospholipase C from Clostridium perfringens stimulates formation and release of platelet-activating factor (PAF-acether) in cultured intestinal epithelial cells (INT 407).
This study demonstrates the ability of phospholipase C from Clostridium perfringens to stimulate the generation of platelet-activating factor (PAF-acether) in cultured intestinal epithelial cells (INT 407). Cells were exposed to phospholipase C for up to 60 min, and the content of PAF-acether within the cells and in the extracellular medium was determined. Phospholipase C caused a time-dependent formation of PAF-acether within the cells and also release of PAF-acether to the medium. In contrast, phospholipase C did not affect the cellular acetylhydrolase activity or the ability of the cells to metabolize extracellularly added 14C-PAF-acether. These findings suggest the possibility that intestinal epithelial cells, when stimulated with a naturally occurring intestinal bacterial toxin, generate and release PAF-acether. The possibility that this might contribute to the pathophysiology of inflammatory bowel disease is discussed.
Gustafson
C
C
Clinical Research Center, Faculty of Health Sciences, Linköping University, Sweden.
Kald
B
B
Sjödahl
R
R
Tagesson
C
C
D002460:Cell Line; D003016:Clostridium perfringens / Q000201:enzymology*; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology; D010972:Platelet Activating Factor / Q000096:biosynthesis*; D010738:Type C Phospholipases / Q000494:pharmacology*
D010972:Platelet Activating Factor; D010738:Type C Phospholipases