Influence of immobilization on properties of polyphosphate glucokinase.
Polyphosphate glucokinase from Mycobacterium tuberculosis H37Ra was covalently bound to the glutaraldehyde-activated collagen coating silica gel, to the nylon, and to the CNBr-activated Sepharose. After immobilization kinetic properties of the enzyme were altered.
Kowalczyk
T
T
Department of Biochemistry and Drug Metabolism, Medical Academy, Lublin, Poland.
Szymona
O
O
Wolski
T
T
D002846:Chromatography, Affinity; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009169:Mycobacterium tuberculosis / Q000201:enzymology; D010770:Phosphotransferases / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity
D004800:Enzymes, Immobilized; D010770:Phosphotransferases; C024812:polyphosphate-glucose phosphotransferase