Primary structure and functional expression of a glutaminyl cyclase.
A glutaminyl cyclase (QC) that is probably involved in the biosynthesis of pyroglutamyl peptides such as gonadotropin-releasing hormone and thyrotropin-releasing hormone has been purified to homogeneity from bovine anterior pituitary. On the basis of N-terminal sequence analysis, a 2088-base-pair cDNA clone was isolated from a bovine anterior pituitary library. From the nucleotide sequence of this clone, the primary structure of a 330-residue protein and a preceding 31-residue prepropeptide sequence was deduced. By transfection of COS-7 monkey cells with a QC cDNA/pCDM8 vector construct, QC activity was expressed. Hybridization with mRNAs of various bovine tissues revealed expression of QC mainly in brain tissue.
Pohl
T
T
Department of Molecular Neuroendocrinology, Max Planck Institute for Experimental Medicine, Göttingen, Federal Republic of Germany.
Zimmer
M
M
Mugele
K
K
Spiess
J
J
D000217:Acyltransferases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000595:Amino Acid Sequence; D019881:Aminoacyltransferases; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D015180:Electrophoresis, Gel, Two-Dimensional; D015723:Gene Library; D007202:Indicators and Reagents; D007700:Kinetics; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D009928:Organ Specificity; D010446:Peptide Fragments / Q000302:isolation & purification; D010455:Peptides / Q000138:chemical synthesis; D010903:Pituitary Gland, Anterior / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D014162:Transfection
D007202:Indicators and Reagents; D015345:Oligonucleotide Probes; D010446:Peptide Fragments; D010455:Peptides; D012333:RNA, Messenger; D000217:Acyltransferases; D019881:Aminoacyltransferases; C059280:glutaminyl-peptide cyclotransferase