title: Drug-susceptibility and isolation of a plasmid in Haemophilus (Actinobacillus) pleuropneumoniae.
abstract: We isolated 56 Haemophilus (Actinobacillus) pleuropneumoniae strains from the pneumonic porcine lung tissues and tested them for antimicrobial susceptibility. Two drug-resistant strains were obtained. One, named KH-265, was resistant to streptomycin (SM) and sulfonamide (SA), and the other, named KH-195, was resistant to tetracycline (TC). The minimum inhibitory concentrations (MICs) of drugs for resistant strains were 100 micrograms/mliters for SM, 3200 micrograms/mliters for SA, and 12.5 micrograms/mliters for TC. KH-265 possessed a 8.3Kb nonconjugative plasmid, pMS260, encoding SM and SA resistance, which was transformable to E. coli strains. pMS260 belonged to none of 14 incompatibility groups including Inc. P and Inc. Q, so far tested. It was mobilizable to various causative strains for respiratory infections, Pseudomonas aeruginosa, Bordetella bronchiseptica, Pasteurella multocida and Haemophilus pleuropneumoniae, by RP4 (Inc. P) plasmid.
authors.affiliation: Central Meat Inspection Laboratory, Prefecture of Gunma, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ishii
authors.forename: Y
authors.initials: Y
authors.lastname: Nakasone
authors.forename: S
authors.initials: S
authors.lastname: Shigehara
authors.forename: K
authors.initials: K
authors.lastname: Honma
authors.forename: Y
authors.initials: Y
authors.lastname: Araki
authors.forename: S
authors.initials: S
authors.lastname: Iyobe
authors.forename: H
authors.initials: H
authors.lastname: Hashimoto
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics; D006190:Haemophilus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D007613:Kanamycin Resistance; D007769:Lactams; D008168:Lung / Q000382:microbiology*; D010403:Penicillin Resistance; D010957:Plasmids / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D013307:Streptomycin / Q000494:pharmacology; D013449:Sulfonamides / Q000494:pharmacology; D013552:Swine; D014161:Transduction, Genetic; D014169:Transformation, Bacterial; D014295:Trimethoprim / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D007769:Lactams; D013449:Sulfonamides; D014295:Trimethoprim; D013307:Streptomycin