Gelatinase secretion by glomerular epithelial cells.
A significant increase in gelatinolytic activity was observed in cultures of glomeruli from Heymann nephritic rats. Zymography of the culture medium indicated that the main gelatinase species has a molecular weight of 98 kilodaltons (kDa) and the characteristics of metalloproteinase. The 98-kDa gelatinase was detected in the culture medium of glomerular epithelial cells but not in those of endothelial and mesangial cells. Interleukin-1 beta, tumor necrosis factor-alpha and lipopolysaccharide dose-dependently increased gelatinase production by epithelial cells. These results suggest that the gelatinase secreted by cytokine-stimulated glomerular epithelial cells participates in the pathological process of Heymann nephritis.
Watanabe
K
K
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Kinoshita
S
S
Nakagawa
H
H
D000818:Animals; D002478:Cells, Cultured; D004848:Epithelium / Q000187:drug effects / Q000503:physiopathology; D018093:Gelatinases; D005921:Glomerulonephritis / Q000503:physiopathology*; D007375:Interleukin-1 / Q000494:pharmacology; D007678:Kidney Glomerulus / Q000378:metabolism* / Q000503:physiopathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D010434:Pepsin A / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D007375:Interleukin-1; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D010434:Pepsin A; D018093:Gelatinases