Epidermal growth factor inhibits both cholecystokinin octapeptide-induced inositol 1,4,5-trisphosphate production and [CA2+]i increase in rat pancreatic acinar cells.
We have studied the effects of epidermal growth factor (EGF) on both cholecystokinin octapeptide (CCK-OP)-induced inositol-1,4,5 trisphosphate (IP3) production and on cytosolic free calcium concentrations [Ca2+]i by fluorescence measurements in fura-2-loaded pancreatic acini. Our data show that EGF inhibits CCK-OP induced IP3 production by 40 +/- 9% and decreases CCK-OP induced rise in cytosolic Ca2+ by 41 +/- 9%. These data indicate that activation of EGF receptors leads to inhibition of CCK-OP induced stimulation of phospholipase C (PLC).
Pröfrock
A
A
Max-Planck-Institut für Biophysik, Frankfurt am Main, Fed. Rep. of Germany.
Piiper
A
A
Eckhardt
L
L
Schulz
I
I
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D019204:GTP-Binding Proteins / Q000378:metabolism; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D007700:Kinetics; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D012844:Sincalide / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D004815:Epidermal Growth Factor; D015544:Inositol 1,4,5-Trisphosphate; D019204:GTP-Binding Proteins; D012844:Sincalide; D002118:Calcium