[The kinetics of cell-mediated cytotoxicity to schistosomula of Schistosoma japonicum with sera from infected mice at different duration].
The tests of antibody-dependent cell-mediated cytotoxicity (ADCC) were performed to observe the kinetics of in vitro killing of Schistosoma japonicum schistosomula mediated by eosinophils, macrophages or neutrophils with infected mouse sera of different duration. The results showed that when complement was not involved, the killing rates of schistosomula mediated by eosinophils or macrophages began to increase significantly at 4 wk post-infection, reached a plateau in 5-7 wk and 6-8 wk respectively, and then declined to the levels of that at 4 wk till 11 wk. In case of neutrophils, there was no significant cytotoxicity detected. When complement was involved, all the three effector cells could mediate cytotoxicity to schistosomula, with the killing rates higher than those without complement at the correspondent time intervals. These indicate that ADCC appears to be an important ingredient in the acquired resistance to S. japonicum, and demonstrate that eosinophil- and macrophage-mediated cytotoxicity to schistosomula is complement-independent, whereas neutrophil-mediated cytotoxicity to schistosomula is complement-dependent. This method may be of some value for choosing optimal time for vaccination and estimating the effectiveness of a candidate vaccine.
Tao
Y W
YW
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine.
Lui
S X
SX
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D003165:Complement System Proteins; D004804:Eosinophils / Q000276:immunology; D007106:Immune Sera; D007111:Immunity, Cellular; D008264:Macrophages / Q000276:immunology; D051379:Mice; D009504:Neutrophils / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D012549:Schistosoma japonicum / Q000276:immunology*
D007106:Immune Sera; D003165:Complement System Proteins