Surface expression of CD-4 does not predict susceptibility to infection with HIV-1 in human monocyte hybridomas.
In contrast to T-cell lines, where CD-4 expression may predict susceptibility to HIV infection, in monocyte hybridomas, presence or absence of surface CD-4 does not appear to be the determining factor of susceptibility to HIV infection. One clone, 20, was documented to be CD-4 negative by surface immunofluorescence as well as by immunoprecipitation. Both CD-4+ and CD-4- human monocyte hybridomas, representative of peripheral blood monocytes were readily infected with HIV (strains IIIB and BR-1 and a variety of patient isolates) as assessed by p24 Ag secretion reverse transcriptase activity and in situ hybridization. Infection occurred in the absence of antibody to HIV suggesting a non Fc mediated process as had been previously described. These data suggest that alternative mechanisms, such as non-specific phagocytosis, may exist for entry of HIV into peripheral blood monocytes. Given these findings, treatment for AIDS, such as the use of soluble CD-4, may not be effective long term, as monocyte infection may still occur and serve as a reservoir for subsequent viral infection of T cells.
Sperber
K
K
Division of Clinical Immunology, Mount Sinai, Medical Center, New York, New York 10029.
Shaked
A
A
Posnett
D N
DN
Hirschman
S Z
SZ
Bekesi
G
G
Mayer
L
L
D015704:CD4 Antigens / Q000032:analysis* / Q000502:physiology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology / Q000382:microbiology*; D007371:Interferon-gamma / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D008213:Lymphocyte Activation; D016403:Lymphoma, Large B-Cell, Diffuse / Q000473:pathology; D010587:Phagocytosis; D015728:Receptors, HIV; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000382:microbiology
D015704:CD4 Antigens; D015728:Receptors, HIV; D007371:Interferon-gamma