title: Identification of proteins whose synthesis in Saccharomyces cerevisiae is induced by DNA damage and heat shock.
abstract: Protein synthesis in Saccharomyces cerevisiae after exposure to ultraviolet light (UV) was examined by two-dimensional gel electrophoresis of pulse-labelled proteins. The synthesis of 12 distinct proteins was induced by treatment with UV doses of 10-200 J/m2. The induced proteins differed in the minimum dose necessary for induction, the maximum dose at which induction still occurred and the constitutive level present in unirradiated cells. A chemical mutagen, 4-nitroquinoline-1-oxide, induced synthesis of the same proteins. Induction after UV treatment was observed in seven different yeast strains, including three mutants deficient in DNA repair. Synthesis of five of the proteins was also induced by brief heat shock treatment. These five proteins may be members of a family of proteins whose synthesis is regulated by two different pathways responding to different types of stress.
authors.affiliation: Department of Biology, University of California, San Diego, La Jolla 92093.
authors.forename: J
authors.initials: J
authors.lastname: Gailit
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D004249:DNA Damage; D004271:DNA, Fungal / Q000528:radiation effects*; D005656:Fungal Proteins / Q000032:analysis / Q000096:biosynthesis*; D006360:Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D006360:Heat-Shock Proteins; D015112:4-Nitroquinoline-1-oxide