A new qualitative method for detecting IgD in unconcentrated cerebrospinal fluid.
We report a method which is capable of demonstrating the isoelectric focusing (IEF) pattern of immunoglobulin D in unconcentrated cerebrospinal fluid (CSF) samples containing as little as 0.1-0.5 ng of total IgD. The method used was an immuno-sandwich technique, with alkaline phosphatase enzyme amplification. Oligoclonal and polyclonal IgD patterns were seen in CSF samples. No cross-reactivity with other immunoglobulins (IgG, IgA and IgM) was detected.
Mavra
M
M
Department of Special Chemical Pathology, National Hospital for Neurology and Neurosurgery, London, U.K.
Luxton
R
R
Keir
G
G
Miletic
V
V
Thompson
E J
EJ
D006801:Humans; D006863:Hydrogen-Ion Concentration; D007072:Immunoglobulin D / Q000134:cerebrospinal fluid*; D007525:Isoelectric Focusing
D007072:Immunoglobulin D