A short synthetic adaptor as second-strand primer in the construction of cDNA libraries by the vector-primer method.
The construction of high quality cDNA libraries is one of the most important yet technically challenging procedures in the study of gene structure and function. The present report presents a simplification of the classical Okayama-Berg protocol for the construction of plasmid libraries. The introduction of a short synthetic oligonucleotide as a second-strand adaptor facilitates the optimization of library construction and allows for quick adaptation of almost any vector as a cDNA cloning vehicle.
Boel
E
E
Molecular Genetics, Bioscience Novo Nordisk A/S, Bagsvoerd, Denmark.
Hjorth
A L
AL
Møller
K B
KB
Møller
P H
PH
D001483:Base Sequence; D004247:DNA; D003854:Deoxyribonucleotides; D015723:Gene Library; D008969:Molecular Sequence Data; D010957:Plasmids
D003854:Deoxyribonucleotides; D004247:DNA