Biotinylated peptides/proteins. II. Identification of biotinylated lysyl phenylthiohydantoins.
The identification and characterization of biotinylated lysyl residues in a polypeptide chain by automated sequence analysis is described. An in depth analytical study was conducted for the delineation of N epsilon modification of lysyl residues with N-hydroxysuccinimide esters of biotin and 6-aminohexanoic biotin. Confirmation of the structure of the phenylthiohydantoin derivatives of N epsilon biotinylated lysine was achieved by mass spectrometry. The analytical study focused on the identification of biotinylated lysine-4 of neuropeptide Y which served as a model peptide for the analytical procedures detailed.
Smith
J S
JS
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.
Miller
B T
BT
Kurosky
A
A
D000595:Amino Acid Sequence; D001710:Biotin / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008239:Lysine / Q000737:chemistry; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D010669:Phenylthiohydantoin / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
D009478:Neuropeptide Y; D010455:Peptides; D011506:Proteins; D010669:Phenylthiohydantoin; D001710:Biotin; D008239:Lysine