Purification and characterization of a Bacteroides loeschei adhesin that interacts with procaryotic and eucaryotic cells.
The adhesin of Bacteroides loeschei PK1295 that mediates coaggregation with Streptococcus sanguis 34 and hemagglutination of erythrocytes was purified to electrophoretic homogeneity. The lectinlike protein has an estimated native Mr of 450,000 and consists of six subunits of identical molecular weight (Mr 75,000). The purified adhesin appears to be a basic protein with a pI between 7.4 and 8.0. Amino acid and N-terminal sequence analyses were carried out with the purified protein. These indicated that the protein contains a large number of Asx and Glx residues as well as basic amino acid residues. The binding site of the pure adhesin retained its native configuration during purification. When preincubated with streptococcal partner cells at pH 4.6, the adhesin prevented B. loeschei cells from coaggregating with the streptococci. An adhesin preparation adjusted to a pH of 6.8 rapidly agglutinated both streptococci and neuraminidase-treated erythrocytes. Galactosides inhibited the agglutination reactions.
London
J
J
Laboratory of Microbial Ecology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.
Allen
J
J
D018829:Adhesins, Bacterial; D018830:Adhesins, Escherichia coli; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D001439:Bacteroides / Q000502:physiology*; D002449:Cell Aggregation / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D006384:Hemagglutination; D007525:Isoelectric Focusing; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009439:Neuraminidase; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep; D013298:Streptococcus sanguis / Q000502:physiology
D018829:Adhesins, Bacterial; D018830:Adhesins, Escherichia coli; D000906:Antibodies; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D046911:Macromolecular Substances; C063910:adhesin, Bacteroides; D009439:Neuraminidase