Dietary modulation of cytochrome P450 in the small intestinal epithelium.
Cytochrome P450 function was compared in rats maintained on cereal-based chow or a purified diet that simulates nutrient levels present in the chow. Ethoxycoumarin O-deethylase activity was increased in small intestine (15-fold), colon (2.5-fold) and liver (1.6-fold) by cereal chow. Ethoxyresorufin O-deethylase activity was also increased by cereal chow (75- and 2.5-fold in small intestine and liver, respectively). Cytochrome P450IAI, detected by Western blotting, was observed in intestinal microsomes isolated from chow-fed rats. These data underscore the importance of dietary considerations when evaluating studies of xenobiotics that undergo 'first pass' cytochrome P450-dependent metabolism in the small intestine.
Rosenberg
D W
DW
Department of Metabolism/Pharmacology, Rockefeller University, New York, N.Y.
D015660:7-Alkoxycoumarin O-Dealkylase / Q000378:metabolism; D000818:Animals; D001571:Benzoflavones / Q000494:pharmacology; D003106:Colon / Q000201:enzymology; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004032:Diet; D004790:Enzyme Induction / Q000187:drug effects; D004848:Epithelium / Q000201:enzymology; D007421:Intestine, Small / Q000201:enzymology*; D008099:Liver / Q000201:enzymology; D008297:Male; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D019324:beta-Naphthoflavone
D001571:Benzoflavones; D019324:beta-Naphthoflavone; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D015660:7-Alkoxycoumarin O-Dealkylase; D019363:Cytochrome P-450 CYP1A1