Nucleotide sequence and northern analysis of a bovine major histocompatibility class II DR beta-like cDNA.
A 1.2-kb bovine DR beta-like cDNA clone (BoLA-DRB3) was isolated from a peripheral blood lymphocyte cDNA library utilizing a human DR beta cDNA as a probe. BoLA-DRB3 was found to have a high degree of nucleotide sequence similarity (96.8%) with a previously sequenced bovine DR beta-like gene (A1). It is believed that BoLA-DRB3 and A1 represent distinct alleles of one of the three bovine DR beta-like loci. Sequence comparison of BoLA-DRB3 with genes representing the other two bovine DR beta-like loci resulted in moderate degrees of sequence similarities (83.1% and 86.3%, respectively). Comparison of the relative abundance of RNA transcripts of the three bovine DR beta-like loci by Northern analysis of lymphocyte RNA indicated that BoLA-DRB3 is the most actively transcribed of the three bovine DR beta-like genes. Based on these results we suggest that of the three DR beta-like loci thus far identified in the bovine, only one is actively transcribed.
Burke
M G
MG
USDA, US Meat Animal Research Center, Clay Center, Nebraska 68933-0166.
Stone
R T
RT
Muggli-Cockett
N E
NE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002417:Cattle / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D015342:DNA Probes / Q000235:genetics; D005802:Genes, MHC Class II / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D006684:HLA-DR Antigens