[High velocity mechanical injection of foreign DNA into fish oocytes].
High-velocity tungsten microprojectiles were used to introduce into fertilized eggs of loach (Misgurnus fossilis), Rainbow trout (Salmo gairdneri Rich.) and Zebra fish (Brachydanio rerio) DNA sequences of beta-galactosidase and neomycin phosphotransferase genes. No more than 30% of fish oocytes died as a result of bombardment. Experiments revealed marked activity of both enzymes in developing fishes. Neo gene DNA sequences were found in total danio DNA using PCR technique.
Kolesnikov
V A
VA
Alimov
A A
AA
Barmintsev
V A
VA
Beniumov
A O
AO
Zelenina
I A
IA
Krasnov
A M
AM
Dzhabur
R
R
Zelenin
A V
AV
D000818:Animals; D003531:Cypriniformes / Q000235:genetics*; D004247:DNA / Q000008:administration & dosage*; D019868:Kanamycin Kinase; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism; D010770:Phosphotransferases / Q000235:genetics; D014337:Trout / Q000235:genetics*; D014414:Tungsten; D015027:Zebrafish / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
D004247:DNA; D010770:Phosphotransferases; D019868:Kanamycin Kinase; D001616:beta-Galactosidase; D014414:Tungsten
Vysokoskorostnaia mekhanicheskaia in'ektsiia chuzherodnoĭ DNK v iaĭtsekletki ryby.