Inhibition by loperamide of chloride transport across canine cultured tracheal epithelium.
We studied the effect of loperamide on electrical properties of canine cultured tracheal epithelium by Ussing's short-circuited technique. Addition of loperamide to the mucosal bath decreased the short-circuit current, transepithelial potential difference and conductance, whereas submucosal loperamide had no effect. This inhibitory effect was attenuated by furosemide, diphenylamine-2-carboxylate and Cl(-)-free medium but not by amiloride or naloxone. Thus, loperamide may selectively inhibit Cl- secretion across airway epithelium through a non-opiate-dependent mechanism.
Tamaoki
J
J
First Department of Medicine, Tokyo Women's Medical College, Japan.
Sakai
N
N
Isono
K
K
Takizawa
T
T
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002712:Chlorides / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D004285:Dogs; D004594:Electrophysiology; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005665:Furosemide / Q000494:pharmacology; D008139:Loperamide / Q000494:pharmacology*; D008297:Male; D014132:Trachea / Q000187:drug effects / Q000378:metabolism; D062367:ortho-Aminobenzoates / Q000494:pharmacology
D002712:Chlorides; D062367:ortho-Aminobenzoates; D008139:Loperamide; D000584:Amiloride; D005665:Furosemide; C001209:fenamic acid; D000242:Cyclic AMP