Thermal behaviour of lymphocyte membrane: ESR investigation.
The order parameter, S, of the plasma membrane of in toto human peripheral blood lymphocytes was obtained by electron spin resonance spectroscopy in the temperature range 25-41 degrees C. This membrane is completely in the liquid crystalline state above 31 degrees C. In presence of the antigen ETB from Staphylococcus aureus at the concentration of 4 micrograms/3 x 10(7) cells an overall decrease of the order parameter for this membrane is observed. The decrease of S is followed by an upwards shift at about 35 degrees C of the temperature of the liquid crystalline state.
Mazzuca
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Dipartimento di Fisica, Laboratorio di Biofisica Molecolare Università della Calabria, Arcavacata di Rende, Italy.
Sportelli
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D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D004768:Enterotoxins / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure*; D013211:Staphylococcus aureus; D013816:Thermodynamics
D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal