title: Dynamic fluctuations in blood of thrombin/antithrombin III complex (TAT).
abstract: The dynamic fluctuation of thrombin-antithrombin III complex (TAT) was studied in blood obtained during the daytime (at 9 AM, noon, 3 PM), during extracorporeal circulation and during the course of disseminated intravascular coagulation (DIC), to certify whether the level of TAT in blood can reflect the generation of thrombin. In 10 healthy male volunteers, the mean values of TAT (micrograms/liter) were 1.74 (+/- 1.36) at 9 AM, 1.22 (+/- 0.47) at noon, and 1.25 (+/- 0.68) at 3 PM. TAT did not show a daytime fluctuation, unlike fibrinolytic factors. The mean values of TAT in 38 hemodialyzed patients were 4.83 (+/- 2.8) before the initiation, 6.59 (+/- 4.39) in the first hour, and 13.42 (+/- 10.96) at the end of a dialysis session. In 20 patients undergoing open heart surgery, the mean value of TAT was increased during cardiopulmonary bypass (CPB) and decreased with time after the end of surgery. The fibrinopeptide A (FPA) value was increased with TAT during CPB but achieved a maximum level immediately after heparin neutralization by protamine. In 20 patients with DIC, the values of TAT varied from 5.8 to 297 micrograms/liter in the blood at the onset of DIC. In seven of eight patients treated with low-molecular-weight heparin (LMW-H), the values of TAT and FPA were lower 24 hr after LMW-H than before the treatment. These results suggest that the level of TAT in blood reflected the formation of thrombin and could serve as a sensitive parameter of activated coagulation in circulating blood.
authors.affiliation: Second Department of Internal Medicine, School of Medicine, Mie University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Deguchi
authors.forename: M
authors.initials: M
authors.lastname: Noguchi
authors.forename: E
authors.initials: E
authors.lastname: Yuwasaki
authors.forename: T
authors.initials: T
authors.lastname: Endou
authors.forename: A
authors.initials: A
authors.lastname: Deguchi
authors.forename: H
authors.initials: H
authors.lastname: Wada
authors.forename: S
authors.initials: S
authors.lastname: Murashima
authors.forename: M
authors.initials: M
authors.lastname: Nishikawa
authors.forename: S
authors.initials: S
authors.lastname: Shirakawa
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D000328:Adult; D000990:Antithrombin III / Q000378:metabolism*; D006348:Cardiac Surgical Procedures; D002940:Circadian Rhythm; D004211:Disseminated Intravascular Coagulation / Q000097:blood*; D005112:Extracorporeal Circulation; D006801:Humans; D008297:Male; D010447:Peptide Hydrolases / Q000378:metabolism*; D006435:Renal Dialysis
chemical_list: C046193:antithrombin III-protease complex; D000990:Antithrombin III; D010447:Peptide Hydrolases