1-Hexylcarbamoyl-5-fluorouracil alters the expression of heat shock protein in HeLa cells.
We determined the effect of 1-hexylcarbamoyl-5-fluorouracil (HCFU), a masked compound of 5-fluorouracil, on the expression of heat shock protein (HSP) in heat-treated HeLa cells. We used a monoclonal anti-72-kDa heat shock protein antibody. At 37 degrees C, HSP was predominant in the cytoplasm of cells and heating to 43 degrees C for 30 min increased the synthesis of HSP in the nucleus. When HeLa cells were treated with heat and HCFU, at a concentration which showed evidence of synergy, nuclear staining of the cells decreased. Thus, the altered expression of HSP by HCFU is related to a synergism between heat and HCFU.
Kusumoto
T
T
Cancer Center, Kyushu University Hospital, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Maehara
Y
Y
Sakaguchi
Y
Y
Emi
Y
Y
Kohnoe
S
S
Sugimachi
K
K
D002478:Cells, Cultured; D002999:Clone Cells / Q000187:drug effects / Q000378:metabolism; D005472:Fluorouracil / Q000031:analogs & derivatives* / Q000494:pharmacology; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D013234:Stem Cells / Q000187:drug effects
D006360:Heat-Shock Proteins; C017367:carmofur; D005472:Fluorouracil