Sugar analysis of glycoproteins and glycolipids after methanolysis by high-performance liquid chromatography with pulsed amperometric detection.
A procedure for the analysis of the monosaccharide composition of glycoproteins and glycolipids by methanolysis and high-performance liquid chromatography with pulsed amperometric detection is described. The advantage over previous methods is the analysis of underivatized methyl glycosides of all glycoconjugate monosaccharides including sialic acid and uronic acid in a single chromatographic step at the subnanomolar level.
Lampio
A
A
Department of Biochemistry, University of Helsinki, Finland.
Finne
J
J
D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006017:Glycolipids / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D006801:Humans; D000432:Methanol; D008759:Methylglycosides / Q000032:analysis; D012794:Sialic Acids / Q000032:analysis; D014574:Uronic Acids / Q000032:analysis
D002241:Carbohydrates; D006017:Glycolipids; D006023:Glycoproteins; D008759:Methylglycosides; D012794:Sialic Acids; D014574:Uronic Acids; D000432:Methanol