title: Central nervous system involvement during infection by Phlebovirus toscana of residents in natural foci in central Italy (1977-1988).
abstract: Central nervous system (CNS) involvement was detected during infection caused by the sand fly-transmitted Phlebovirus Toscana. One hundred fifty-five cases of Toscana virus-associated meningitis or meningoencephalitis were identified in a survey that lasted ten years, conducted in two regions of central Italy. Diagnosis was performed by different serologic tests. A combination of hemagglutination-inhibition and plaque-reduction neutralization or indirect immunofluorescence for IgM, and enzyme-linked immunosorbent assays for IgM were considered the most suitable tests for the diagnosis of Toscana virus infection. A few strains of Toscana virus were isolated from the cerebrospinal fluid of seropositive patients. Toscana virus-associated CNS disease occurred during the summer, reaching a peak value in August, when the maximum activity of the sand fly vector occurs and virus isolates are obtained in their natural foci. The results suggest that Toscana virus should be considered as a possible cause of CNS disease in Mediterranean countries where sand flies of the genus Phlebotomus are known to be present.
authors.affiliation: Laboratory of Virology, Istituto Superiore di Sanita, Rome, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Nicoletti
authors.forename: P
authors.initials: P
authors.lastname: Verani
authors.forename: S
authors.initials: S
authors.lastname: Caciolli
authors.forename: M G
authors.initials: MG
authors.lastname: Ciufolini
authors.forename: A
authors.initials: A
authors.lastname: Renzi
authors.forename: D
authors.initials: D
authors.lastname: Bartolozzi
authors.forename: P
authors.initials: P
authors.lastname: Paci
authors.forename: F
authors.initials: F
authors.lastname: Leoncini
authors.forename: P
authors.initials: P
authors.lastname: Padovani
authors.forename: E
authors.initials: E
authors.lastname: Traini
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D002044:Bunyaviridae Infections / Q000453:epidemiology / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007558:Italy / Q000453:epidemiology; D008297:Male; D008587:Meningitis, Viral / Q000453:epidemiology / Q000382:microbiology*; D008590:Meningoencephalitis / Q000453:epidemiology / Q000382:microbiology*; D009500:Neutralization Tests; D016856:Phlebovirus / Q000276:immunology* / Q000302:isolation & purification; D012621:Seasons
chemical_list: D000914:Antibodies, Viral