title: Identification of 11-cis and all-trans-retinal in the photoreceptive organelle of a flagellate green alga.
abstract: Isolation of intact photoreceptive organelles (eyespot apparatuses) involved in blue-light mediated photoresponses in a flagellate green alga (Spermatozopsis similis) allowed for the first time the identification of both 11-cis- and all-trans-retinal in a plant cell. Both isomers were identified by HPLC analysis in conjunction with UV spectra. Additionally, reconstitution of a distinct absorption band, centered around 540 nm, was achieved by addition of exogenous 9-cis-retinal to bleached, isolated eyespot apparatuses.
authors.affiliation: Universität zu Köln, Botanisches Institut, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Kreimer
authors.forename: F J
authors.initials: FJ
authors.lastname: Marner
authors.forename: U
authors.initials: U
authors.lastname: Brohsonn
authors.forename: M
authors.initials: M
authors.lastname: Melkonian
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry*; D000460:Chlorophyta / Q000737:chemistry* / Q000502:physiology; D005407:Flagella; D015388:Organelles / Q000737:chemistry* / Q000502:physiology; D010786:Photoreceptor Cells / Q000737:chemistry* / Q000502:physiology; D010788:Photosynthesis / Q000502:physiology; D012172:Retinaldehyde / Q000737:chemistry* / Q000502:physiology
chemical_list: C020932:11-cis-retinal-binding protein; D002352:Carrier Proteins; D012172:Retinaldehyde