title: A short synthetic adaptor as second-strand primer in the construction of cDNA libraries by the vector-primer method.
abstract: The construction of high quality cDNA libraries is one of the most important yet technically challenging procedures in the study of gene structure and function. The present report presents a simplification of the classical Okayama-Berg protocol for the construction of plasmid libraries. The introduction of a short synthetic oligonucleotide as a second-strand adaptor facilitates the optimization of library construction and allows for quick adaptation of almost any vector as a cDNA cloning vehicle.
authors.affiliation: Molecular Genetics, Bioscience Novo Nordisk A/S, Bagsvoerd, Denmark.
authors.forename: E
authors.initials: E
authors.lastname: Boel
authors.forename: A L
authors.initials: AL
authors.lastname: Hjorth
authors.forename: K B
authors.initials: KB
authors.lastname: Møller
authors.forename: P H
authors.initials: PH
authors.lastname: Møller
mesh_terms: D001483:Base Sequence; D004247:DNA; D003854:Deoxyribonucleotides; D015723:Gene Library; D008969:Molecular Sequence Data; D010957:Plasmids
chemical_list: D003854:Deoxyribonucleotides; D004247:DNA