title: Spectrophotometric determination of functional characteristics of protein kinases with coupled enzymatic assay.
abstract: The performance of enzyme-coupled spectrophotometric assay of protein kinases and its merits as compared with the radioisotopic method in characterizing functional activity of protein kinases are described. Kinetic parameters of protein kinase C and protein kinase A so obtained are presented. General application of the spectrophotometric assay in the study of protein kinases is recommended.
authors.affiliation: Institute of Medical Biochemistry and Chemistry, University of Bari, Italy.
authors.forename: Z
authors.initials: Z
authors.lastname: Technikova-Dobrova
authors.forename: A M
authors.initials: AM
authors.lastname: Sardanelli
authors.forename: S
authors.initials: S
authors.lastname: Papa
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology; D002853:Chromatography, Liquid; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011493:Protein Kinase C / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D013057:Spectrum Analysis
chemical_list: D011494:Protein Kinases; D011493:Protein Kinase C