Effects of estramustine on DNA and cell membrane in malignant glioma cells.
Estramustine phosphate (EMP), a complex between estradiol-17 beta and nor-nitrogen mustard, commonly used in treatment of prostatic cancer, also exerts marked antiproliferative effects on cultured human malignant glioma cells. The mechanism of action is unknown but has previously been considered to be mediated through non-DNA targets, specifically via the mitotic spindle, and related to the intact estramustine complex. EMP cytotoxicity was studied on the malignant glioma cell line U-251 MG. A dose-dependent increase in DNA strand breaks was demonstrated at EMP-concentrations ranging 10-40 mg/l. The uptake of 86Rb, used as a tracer for potassium to study ion transport and membrane permeability, was reduced after incubation with EMP. The mean decline in 86Rb accumulation by U-251 MG cells was 12, 20 and 32% at EMP concentrations 10, 20 and 40 mg/l respectively. Scanning electron microscopy gave further evidence for cell membrane damage. In conclusion, EMP seems to affect malignant glioma cells on several vital functions and the results indicate the the cytotoxic potential may at least partially be related to effects on DNA and cell membrane.
von Schoultz
E
E
Department of Oncology, University Hospital, Umeå, Sweden.
Grankvist
K
K
Gustavsson
H
H
Henriksson
R
R
D002462:Cell Membrane / Q000187:drug effects*; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004961:Estramustine / Q000494:pharmacology*; D005910:Glioma / Q000235:genetics / Q000648:ultrastructure; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012414:Rubidium Radioisotopes; D014407:Tumor Cells, Cultured / Q000187:drug effects
D004273:DNA, Neoplasm; D012414:Rubidium Radioisotopes; D004961:Estramustine