Growth of Haemophilus influenzae type b in the presence of bovine aortal endothelial cells.
In serum-free medium in the presence of bovine aortal endothelial cells (BAOEC), Haemophilus influenzae type b was capable of extensive proliferation compared to that in serum-free medium alone. An unidentified low-molecular-mass (less than 2000 kDa) compound(s) was, in part, responsible for this phenomenon. There were changes in the outer-membrane protein profiles between broth-grown (the original inoculum) and BAOEC-grown organisms, particularly in the 45-70 kDa range. Both broth- and BAOEC-grown bacteria were serum sensitive in vitro but could be converted to a serum-resistant phenotype, resembling that found in vivo, by incubation in a serum filtrate.
Langford
P R
PR
Department of Paediatrics, John Radcliffe Hospital, Oxford, UK.
Moxon
E R
ER
D000818:Animals; D001011:Aorta; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry; D001770:Blood Bactericidal Activity; D002417:Cattle; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology; D006193:Haemophilus influenzae / Q000737:chemistry / Q000254:growth & development*; D006801:Humans; D007700:Kinetics
D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media