Aluminofluorides and beryllofluorides as inhibitors of sulphatases. Analogues of hydrogen sulphate?
The inhibition of fluoride of sulphatase A from ox liver and of the sulphatases of Helix pomatia and Aspergillus oryzae is decreased by EDTA and increased by Al3+ or Be2+, implicating aluminofluorides and beryllofluorides in the reaction. The inhibition, which is reversible, takes several minutes to develop fully and, at least for the sulphatase of H. pomatia, is of a non-linear mixed competitive-non-competitive type. It is suggested that the aluminofluorides and beryllofluorides are acting as analogues of HSO4-. If so, then this behaviour must be considered, as well as their role as analogues of phosphate, in interpreting the effects of these compounds in intact cells.
Roy
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Protein Chemistry Group, John Curtin School of Medical Research, Australian National University, Canberra.
D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D001236:Aspergillus oryzae / Q000201:enzymology; D001608:Beryllium / Q000494:pharmacology*; D002417:Cattle; D004492:Edetic Acid / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology*; D006372:Helix, Snails / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D012969:Sodium Fluoride / Q000494:pharmacology; D013429:Sulfatases / Q000037:antagonists & inhibitors*
D012969:Sodium Fluoride; D004492:Edetic Acid; D000535:Aluminum; D013429:Sulfatases; D001608:Beryllium; D005459:Fluorides