The effects of alpha-alpha cross-linked hemoglobin on the feeding and locomotor activity of rats.
The feeding and locomotor activities of rats were used as an assay for the potentially toxic effects of an oxygen-carrying blood substitute. Rats lived in individual cages where they could feed ad lib by pressing a lever once for each small food pellet, drink water, or run in a wheel; a 12-h light/dark cycle was continuously in effect. After being anesthetized and hemorrhaged one-third of their total blood volume, individual rats were resuscitated with one of the following fluids: their own shed blood (OB), bis(3,5-dibromosalicylfumarate) alpha-alpha cross-linked hemoglobin (HbXL), human serum albumin (HSA), or Ringer's lactate (RL). Rats in a fifth group were not resuscitated (NR). During the dark period on the day of hemorrhage, the food intake and running activity of rats in all groups decreased. Food intake and locomotor activity of rats in the HbXL, NR and OB groups were more suppressed than the HSA or RL groups. The food intake of rats in the HbXL and NR groups remained significantly more suppressed during the dark period of the first recovery day; running continued to be suppressed in the HbXL group on the first recovery day, but not the second recovery day. In an effort to determine the extent to which the rats in the HbXL group were impaired, an increasing number of lever presses was required for each food pellet beginning with recovery day number 3 for all treatment groups. As the ratio of presses per pellet was increased, food intake decreased and running increased for all groups; no differences between groups were significant.(ABSTRACT TRUNCATED AT 250 WORDS)
Bauman
R A
RA
Department of Medical Neurosciences Walter Reed Army Institute of Research, WRAMC, Washington, DC 20307-5100.
Przybelski
R J
RJ
Bounds
M J
MJ
D000818:Animals; D001070:Appetitive Behavior / Q000187:drug effects / Q000502:physiology; D001241:Aspirin / Q000031:analogs & derivatives* / Q000494:pharmacology; D001802:Blood Substitutes / Q000633:toxicity*; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D002940:Circadian Rhythm / Q000187:drug effects / Q000502:physiology; D003432:Cross-Linking Reagents / Q000633:toxicity*; D004435:Eating / Q000187:drug effects / Q000502:physiology; D006470:Hemorrhage / Q000097:blood; D008297:Male; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains
D001802:Blood Substitutes; D003432:Cross-Linking Reagents; C021630:bis(3,5-dibromosalicyl)fumarate; D001241:Aspirin