[Optimal methods for peripheral blood progenitor assay and its relevance in the blood cell autotransplantation].
Methylcellulose culture study revealed that the potency of recombinant granulocyte-colony stimulating factor (G-CSF) or granulocyte/macrophage (GM)-CSF to support the growth of purified target cell populations from peripheral blood of patients undergoing peripheral blood stem cell (PBSC) autotransplantation was inferior to that of PHA-stimulated lymphocyte conditioned medium (PHA-LCM) or interleukin-3 (IL-3). In liquid-suspension limiting dilution assay the cells responded directly to IL-3 by proliferation with single-hit kinetics. Hence, optimal culture for PBSC should include the use of PHA-LCM or IL-3. Further study disclosed a linear relationship between the number of CFU-GM infused into the patients and days required to achieve a granulocyte count above 0.5 x 10(9)/l.
Ninomiya
T
T
School of Medical Sciences, University of Tokushima.
Takaue
Y
Y
Watanabe
T
T
Azekawa
S
S
Hirao
A
A
Saito
S
S
Abe
T
T
Nakanishi
J
J
Kawano
Y
Y
Kuroda
Y
Y
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003114:Colony-Forming Units Assay / Q000379:methods; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000473:pathology; D006801:Humans; D007223:Infant; D007938:Leukemia / Q000628:therapy; D008297:Male; D014182:Transplantation, Autologous
D003115:Colony-Stimulating Factors