Solubilization and partial purification of squalene synthase from daffodil microsomal membranes.
A squalene synthase was solubilized from daffodil (Narcissus pseudonarcissus L.) microsomes with CHAPS, a zwitterionic non-denaturating detergent. By successive chromatography on DEAE Sephacel and APP Sepharose a fraction enriched in this enzyme (21-fold) was prepared.
Belingheri
L
L
Laboratoire de Physiologie Cellulaire Végétale, Université de Bordeaux, Talence, France.
Beyer
P
P
Kleinig
H
H
Gleizes
M
M
D002793:Cholic Acids; D002848:Chromatography, DEAE-Cellulose; D003902:Detergents; D013186:Farnesyl-Diphosphate Farnesyltransferase / Q000302:isolation & purification* / Q000378:metabolism; D007425:Intracellular Membranes / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology*; D010713:Phosphatidylcholines / Q000378:metabolism; D010944:Plants / Q000201:enzymology*; D013379:Substrate Specificity
D002793:Cholic Acids; D003902:Detergents; D010713:Phosphatidylcholines; D013186:Farnesyl-Diphosphate Farnesyltransferase; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate