Tyrosine-130 in bullfrog thyroglobulin is a thyroid hormone generating site.
Bullfrog thyroglobulin was digested with lysyl endopeptidase, known to be highly specific to cut the C-terminal side of lysine residue in protein, after reduction and carboxymethylation. We isolated one peptide which lacked the C-terminal lysine, and which corresponds to 103-129 of bovine thyroglobulin sequence. Tyrosine 130 in the mammalian thyroglobulin molecule is known to be an iodination site. These findings suggest that tyrosine 130 in frog thyroglobulin is a thyroid hormone generating site.
Hayashi
H
H
Institute of Endocrinology, Gunma University, Maebashi, Japan.
Ohmiya
Y
Y
Suzuki
S
S
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D011892:Rana catesbeiana; D016415:Sequence Alignment; D012697:Serine Endopeptidases / Q000737:chemistry; D013954:Thyroglobulin / Q000235:genetics / Q000378:metabolism*; D013963:Thyroid Hormones / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
D013963:Thyroid Hormones; D014443:Tyrosine; D013954:Thyroglobulin; D012697:Serine Endopeptidases; C036813:lysyl endopeptidase