Conformation and expression of the albumin gene of young and old rats.
The conformation and expression of the albumin gene in the liver of young (21-) and old (85-week) rats were studied. Digestion of nuclei with MNase shows no differences in the nucleosomal organization in the coding region of the gene in the two ages. The gene has a DNase I hypersensitive site which is distinctly less sensitive in old rats. Its 5'-CCGG-3' sequences are more methylated in the old in which its rate of transcription is also lower.
Singh
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A
Department of Zoology, Banaras Hindu University, Varanasi, India.
Singh
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S
Kanungo
M S
MS
D000375:Aging / Q000235:genetics* / Q000378:metabolism; D000418:Albumins / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D015139:Blotting, Southern; D003850:Deoxyribonuclease I; D005786:Gene Expression Regulation / Q000502:physiology*; D005796:Genes / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D008297:Male; D009690:Nucleic Acid Conformation; D009708:Nucleotidases; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic / Q000235:genetics
D000418:Albumins; D003850:Deoxyribonuclease I; D009708:Nucleotidases