Effect of diabetes on glutamine synthetase expression in rat skeletal muscles.
The regulation of glutamine synthetase expression in muscles from normal and diabetic (streptozotocin-treated) rats was studied. Muscle and body weights were markedly reduced in diabetic animals. Glutamine synthetase activity was significantly (2-fold) elevated 7 days after induction of diabetes. Increased enzyme activity persisted for at least 14 days after induction of diabetes, and it was apparent in both slow (soleus) and fast (plantaris) muscles. The diabetes-induced increase in enzyme activity was reflected in an increased steady-state level of glutamine synthetase mRNA. The increases in glutamine synthetase activity and mRNA level in muscle from diabetic rats were reversed by insulin administration. Increased expression of glutamine synthetase may be important for accelerated glutamine production by muscle from diabetic rats.
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Department of Neurology, University of Maryland School of Medicine, Baltimore 21201.
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D000315:Adrenalectomy; D000818:Animals; D001835:Body Weight; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D015870:Gene Expression; D005974:Glutamate-Ammonia Ligase / Q000235:genetics*; D049528:Insulin, Long-Acting / Q000494:pharmacology; D008297:Male; D009132:Muscles / Q000033:anatomy & histology / Q000187:drug effects / Q000201:enzymology*; D009929:Organ Size; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D049528:Insulin, Long-Acting; D012333:RNA, Messenger; D005974:Glutamate-Ammonia Ligase