Clinical and immunologic response of cattle to administration of a vaccine containing modified-live bovine respiratory syncytial virus.
Healthy yearling beef and dairy cattle were inoculated with a vaccine containing modified-live bovine respiratory syncytial virus (ML-BRSV), and sequential changes in clinical signs of disease, blood leukocyte subsets, BRSV-specific antibody titer, and in vitro lymphocyte blastogenic responses were monitored. Vaccination with ML-BRSV did not cause pyrexia, local or systemic hypersensitivity reaction, or respiratory tract disease. Episodes of leukopenia, abnormalities in lymphocyte subsets, or depression of phytomitogen-induced blastogenic responses were not observed subsequent to vaccination. Exposure to ML-BRSV resulted in at least a 16-fold increase in serum neutralizing antibody titer, with no increase seen in nonvaccinated contact controls. Significant BRSV-specific lymphocyte blastogenic responses were not detected, using one dose of several BRSV antigen preparations in a whole blood culturing system.
Ellis
J A
JA
Department of Veterinary Sciences, College of Agriculture, University of Wyoming, Laramie 82070.
Davis
W C
WC
Talens
L
L
Pratt
D L
DL
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000096:biosynthesis; D015496:CD4-Positive T-Lymphocytes; D002417:Cattle / Q000276:immunology*; D002469:Cell Separation; D005434:Flow Cytometry; D007958:Leukocyte Count / Q000662:veterinary; D008213:Lymphocyte Activation; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer; D050378:T-Lymphocytes, Regulatory; D014765:Viral Vaccines / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014765:Viral Vaccines