title: Growth factors involved in lymphocyte differentiation.
abstract: We report here that an interleukin-3-dependent precursor B-cell line, LyD9, differentiated in vitro into mature B cells, producing immunoglobulin (Ig)M and IgG by co-culture with bone marrow stroma cells. Induced LyD9 cells underwent heterogenous immunoglobulin gene rearrangement and synthesized mRNAs encoding immunoglobulin mu (mu), gamma (gamma) and kappa (kappa) chains. LyD9 was also shown to differentiate into myeloid cells. We have established an interleukin-4-dependent derivative clone K-4 that is an intermediate between myeloid-lymphoid cells and the LyD9 clone. This differentiation required direct contact between LyD9 and stromal cells.
authors.affiliation: Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kinashi
authors.forename: K
authors.initials: K
authors.lastname: Tashiro
authors.forename: K H
authors.initials: KH
authors.lastname: Lee
authors.forename: K
authors.initials: K
authors.lastname: Inaba
authors.forename: K
authors.initials: K
authors.lastname: Toyama
authors.forename: R
authors.initials: R
authors.lastname: Palacios
authors.forename: T
authors.initials: T
authors.lastname: Honjo
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D001853:Bone Marrow / Q000502:physiology; D001854:Bone Marrow Cells; D002450:Cell Communication; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002999:Clone Cells; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D015322:Gene Rearrangement, B-Lymphocyte; D005803:Genes, Immunoglobulin; D006133:Growth Substances / Q000494:pharmacology*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007378:Interleukins / Q000494:pharmacology; D051379:Mice; D008808:Mice, Inbred CBA
chemical_list: D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007378:Interleukins