Histopathology and cell culture characteristics of liver cells from grc- and grc+ rats given diethylnitrosamine.
The histopathological response and cell culture characteristics of liver cells from the R16 (grc-) strain of rats, which carries an MHC-linked deletion, were examined one week after a single intraperitoneal injection of 200 mg/kg body weight diethylnitrosamine (DEN) and were compared with the response of liver cells from wild type (grc+) rats. The DEN exposure induced hydropic/vacuolar changes in the parenchymal cells and a limited proliferation of oval cells in the periportal areas of the livers of both grc+ and grc- rats. Primary culture of collagenase-digested livers consisted of parenchymal, bile ductular and oval-related cells as determined by cell-specific immunohistochemistry. Subpassaged cells from grc+ rats exhibited oval cell ultrastructural morphology, inducible histochemical staining for gamma-glutamyl transpeptidase (GGT), and DEN-associated onset of anchorage-independent growth. Primary cultures of liver cells from R16 rats consistently failed to form cell strains upon subpassage.
Smith
G J
GJ
Carcinogenesis Research Unit, School of Pathology, University of New South Wales, Kensington, Australia.
Kunz
H W
HW
Dunsford
H A
HA
Gill
T J
TJ
D000704:Analysis of Variance; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002448:Cell Adhesion; D002478:Cells, Cultured; D002872:Chromosome Deletion; D004052:Diethylnitrosamine / Q000494:pharmacology / Q000633:toxicity*; D004533:Egtazic Acid / Q000031:analogs & derivatives; D007124:Immunoenzyme Techniques; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008285:Major Histocompatibility Complex / Q000235:genetics; D008957:Models, Genetic; D051381:Rats; D011922:Rats, Mutant Strains; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D002273:Carcinogens; D004052:Diethylnitrosamine; D004533:Egtazic Acid; D005723:gamma-Glutamyltransferase; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid