Immunohistochemical study of creatine kinase isozymes in the human upper limb bud.
Creatine kinase (CK) is involved in the production of ATP and is composed of two immunologically distinct subunits, B (CK-B) and M (CK-M). In the differentiation of myoblasts, the isozyme of CK changes from CK-B to CK-M. In the present study, the expression of CK subunits was studied immunohistochemically in the upper limb bud of human embryos (Carnegie stages 13-21). It was found that CK-B and CK-M immunoreactive cells appeared at stage 15 and at stage 18, respectively.
Shinohara
H
H
Department of Anatomy, Shimane Medical University, Izumo, Japan.
Oguni
M
M
Tanaka
O
O
Kato
K
K
D001132:Arm / Q000196:embryology*; D002454:Cell Differentiation; D003402:Creatine Kinase / Q000032:analysis*; D004622:Embryo, Mammalian / Q000201:enzymology; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes; D009132:Muscles / Q000196:embryology* / Q000201:enzymology
D007527:Isoenzymes; D003402:Creatine Kinase