title: A new monoclonal anti-A antibody BIRMA-1. A potent culture supernatant which agglutinates Ax cells, but does not give undesirable reactions with B cells.
abstract: A monoclonal anti-A antibody, BIRMA-1, has been evaluated and found to be eminently suitable as a blood-grouping reagent. The culture supernatant is potent, avid and specific as demonstrated by its reactivity with a large sample of donor bloods tested manually and on the Olympus PK 7100 automated blood grouping machine. Over 100 cord blood samples were tested manually, and all were correctly grouped using this antibody. No false-positive reactions were obtained with papainized group B cells. Reagent prepared from BIRMA-1 detected most examples of Ax and all other A subgroups and A variants tested. Negative reactions with B(A) cells indicate that it is possible to have an antibody capable of detecting Ax which will not react with B cells from individuals with high levels of galactosyltransferase.
authors.affiliation: West Midlands Regional Transfusion Centre, Birmingham, UK.
authors.forename: D F
authors.initials: DF
authors.lastname: McDonald
authors.forename: J M
authors.initials: JM
authors.lastname: Thompson
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D000918:Antibody Specificity; D004355:Drug Stability; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal