title: Isolation of anonymous, polymorphic DNA fragments from human chromosome 22q12-qter.
abstract: A series of 195 random chromosome 22-specific probes, equivalent to approximately 1% of the size of this chromosome, have been isolated from a chromosome 22-specific bacteriophage lambda genomic library. These probes were mapped to four different regions of chromosome 22 on a panel of five somatic cell hybrids. Restriction fragment length polymorphisms were detected by 28 of the probes mapping to 22q12-qter. Evolutionarily conserved sequences in human, mouse, and Chinese hamster DNA were detected by 12% of the isolated probes.
authors.affiliation: Ludwig Institute for Cancer Research, Stockholm, Sweden.
authors.forename: J P
authors.initials: JP
authors.lastname: Dumanski
authors.forename: A H
authors.initials: AH
authors.lastname: Geurts van Kessel
authors.forename: M
authors.initials: M
authors.lastname: Ruttledge
authors.forename: A
authors.initials: A
authors.lastname: Wladis
authors.forename: N
authors.initials: N
authors.lastname: Sugawa
authors.forename: V P
authors.initials: VP
authors.lastname: Collins
authors.forename: M
authors.initials: M
authors.lastname: Nordenskjöld
mesh_terms: D015139:Blotting, Southern; D002892:Chromosomes, Human, Pair 22; D015342:DNA Probes / Q000302:isolation & purification*; D006801:Humans; D006822:Hybrid Cells; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
chemical_list: D015342:DNA Probes