[Evaluation of Testryp CATT applied to blood samples on filter paper and on diluted blood in a focus of trypanosomiasis due to Trypanosoma brucei gambiense in the Ivory Coast].
The Testryp CATT was performed on dried blood samples on filter-paper and on diluted blood using a microtechnique. This method was applied to both sample collection techniques and was evaluated in parallel with the classical Testryp CATT on whole blood, as described in the instructions provided with the reagents by the manufacturer. A total of 2087 people were tested; 453 samples were tested in the laboratory and 1634 during a field survey in 5 villages of a trypanosomiasis focus in Daloa, Côte d'Ivoire. This study has demonstrated that the Testryp CATT micromethod on either type of sample collection gives results comparable to the Testryp CATT on whole blood. The collection of dried blood samples on filter-paper can be performed by non-specialized staff in trypanosomiasis control programmes of the national health services. In addition, a flask of CATT reagent will allow testing of 6 times more people by the micromethod than by the classical whole-blood method. The micromethod is suitable in the implementation of programmes for the serological surveillance of populations at risk.
Miezan
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T
Projet de REcherches Cliniques sur la Trypanosomiase (PRCT), Daloa Côte d'Ivoire.
Doua
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F
Cattand
P
P
de Raadt
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P
D000372:Agglutination Tests; D000818:Animals; D000913:Antibodies, Protozoan / Q000302:isolation & purification; D006801:Humans; D011934:Reagent Strips; D013048:Specimen Handling / Q000379:methods*; D014347:Trypanosoma brucei gambiense / Q000276:immunology; D014353:Trypanosomiasis, African / Q000276:immunology* / Q000469:parasitology
D000913:Antibodies, Protozoan; D011934:Reagent Strips
Evaluation du Testryp CATT appliqué au sang prélevé sur papier filtre et au sang dilué, dans le foyer de trypanosomiase à Trypanosoma brucei gambiense en Côte d'Ivoire.