Characterization of adult human astrocytes derived from explant culture.
Four different human astrocytic cell lines established from either epilepsy surgical specimens or cerebral white matter obtained during thalamotomy for tremor in a patient with multiple sclerosis were characterized using morphologic analysis, ultrastructural attributes, growth characteristics, and immunocytochemical analysis. Immunocytochemical characterization of cultures indicated a mean of 84% of cells contained cytoplasmic glial fibrillary acidic protein (GFAP): to confirm that GFAP(+) cells also proliferated, bromo-deoxyuridine (BrdU) uptake was measured in cell line. Our method of simplified explant culture allows establishment of astrocytic cell lines from a variety of pathologic substrates using limited amounts of human material.
Estes
M L
ML
Department of Pathology, Cleveland Clinic Foundation, OH 44195.
Ransohoff
R M
RM
McMahon
J T
JT
Jacobs
B S
BS
Barna
B P
BP
D000328:Adult; D001253:Astrocytes / Q000276:immunology / Q000502:physiology / Q000648:ultrastructure*; D001973:Bromodeoxyuridine / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009103:Multiple Sclerosis / Q000503:physiopathology
D005904:Glial Fibrillary Acidic Protein; D003994:Bucladesine; D001973:Bromodeoxyuridine