[Function of direct repeat sequences in DNA replication of hepatitis B virus].
In order to investigate the function of the direct repeat sequences (DR 1 and DR 2) in DNA replication of hepatitis B virus (HBV), the nucleotides in DR 1 and/or DR 2 were substituted by oligonucleotide site-directed mutagenesis. HepG 2 cells were transfected with tandem dimers of the mutants. The results indicate that (1) DR 1 is responsible for the synthesis of pregenome RNA or minus strand DNA. (2) The relative homogeneity between DR 2 and DR 2 is very important in the formation of long cohesive end region and the circulization of HBV DNA. (3) It is first reported that in natural infection HBV DNA has a defective replication route. It gives a 3.2 Kb linear form double strand DNA with uniform structure.
Wang
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Y
Tao
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Q
D004261:DNA Replication; D004279:DNA, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D016297:Mutagenesis, Site-Directed; D012091:Repetitive Sequences, Nucleic Acid / Q000502:physiology*; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral