The role of lipopolysaccharide in complement-killing of Aeromonas hydrophila strains of serotype O:34.
The role of lipopolysaccharide (LPS) in the susceptibility of Aeromonas hydrophila strains of serotype O:34 to non-immune human serum was investigated using isogenic mutants (serum-sensitive), previously obtained on the basis of phage resistance, and characterized for their surface components. The classical complement pathway was found to be principally involved in the serum-killing of these sensitive strains. LPS preparations from serum-resistant or serum-sensitive strains, or purified core oligosaccharides (low-molecular-mass LPS) inactivated both bactericidal and complement activity of whole serum, while the O-antigen molecules (high-molecular-mass LPS) did not. The results indicate that LPS core oligosaccharide composition contributes to complement resistance of A. hydrophila strains from serotype O:34 with moderate virulence.
Merino
S
S
Departamento de Microbiología, Universidad de Barcelona, Spain.
Camprubí
S
S
Tomás
J M
JM
D016980:Aeromonas hydrophila / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D001770:Blood Bactericidal Activity; D003179:Complement C3b / Q000378:metabolism; D015938:Complement Membrane Attack Complex / Q000378:metabolism; D003171:Complement Pathway, Classical; D003165:Complement System Proteins / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007700:Kinetics; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D009154:Mutation; D014774:Virulence
D015938:Complement Membrane Attack Complex; D008070:Lipopolysaccharides; D003179:Complement C3b; D003165:Complement System Proteins