Comparative study of isoproterenol and 4-deoxypyridoxine effects on the isovolumic rat heart model.
Isovolumic rat hearts were perfused for 20 min (T20) or 40 min (T40) with 10(-6) M isoproterenol (ISO), 10(-5) M 4-Deoxypiridoxine (DOP) or with both drugs (ISO-DOP). ISO increased developed pressure 51% and 31%, coronary flow (C.F.) 37% and 52%, heart rate 50% and 60%, lactate production 160% and 76%, and CK activity in the effluent perfusate 182% and 173% at T20 and T40 respectively, whereas glycogen stores decreased by 47% and 77%. DOP needed at least 40 min to increase heart rate 32%, C.F. 40% and to decrease glycogen reserves by 33% and pyridoxal-5-phosphate 13%. In the ISO-DOP group, the ISO inotropic effect was lowered by 23% (T20) and 25% (T40) and DOP reduced ISO induced glycogenolysis.
Lorenzato
M
M
Laboratoire de Pharmacologie, Faculté de Pharmacie, Reims, France.
Millart
H
H
Albert
O
O
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D003326:Coronary Circulation / Q000187:drug effects; D003402:Creatine Kinase / Q000032:analysis; D000242:Cyclic AMP / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D006321:Heart / Q000187:drug effects*; D006339:Heart Rate / Q000187:drug effects; D007545:Isoproterenol / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D006005:Phosphorylases / Q000378:metabolism; D011736:Pyridoxine / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
C004675:4-deoxypyridoxine; D006003:Glycogen; D000242:Cyclic AMP; D006005:Phosphorylases; D003402:Creatine Kinase; D011736:Pyridoxine; D007545:Isoproterenol