title: Phosphatidylinositol-attached 5'-nucleotidase from synaptic plasma membrane of rat brain.
abstract: Synaptic plasma membranes (SPM) of rat brain contained a 5'-nucleotidase that was specifically released by Bacillus thuringiensis phosphatidylinositol-specific phospholipase C (PIPLC). About 30% of the enzyme was readily released and the remainder was less susceptible. Purified 5'-nucleotidase was treated with PIPLC and the resultant enzyme was almost totally partitioned into the detergent-poor phase following phase-separation in Triton X-114 indicating that PIPLC converted the enzyme from an amphipathic to a hydrophilic form. The results suggest that 5'-nucleotidase is anchored into SPM by a covalently attached phosphatidylinositol moiety.
authors.affiliation: Department of Biochemistry, University of Hong Kong.
authors.forename: K M
authors.initials: KM
authors.lastname: Lai
authors.forename: P C
authors.initials: PC
authors.lastname: Wong
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism*; D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism; D066298:In Vitro Techniques; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000378:metabolism*; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000494:pharmacology; D051381:Rats; D013570:Synaptic Membranes / Q000187:drug effects / Q000378:metabolism*
chemical_list: D010716:Phosphatidylinositols; D015720:5'-Nucleotidase; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase