Probestin, a new inhibitor of aminopeptidase M, produced by Streptomyces azureus MH663-2F6. I. Taxonomy, production, isolation, physico-chemical properties and biological activities.
Probestin has been isolated as part of a program designed to find microorganism-produced inhibitors of aminopeptidase M from Streptomyces azureus MH663-2F6. It was purified by use of column chromatography of Amberlite XAD-4, silica gel, YMC-gel, Toyopearl HW-40, YMC D-ODS-5 (HPLC) and then isolated as colorless powders. Probestin is competitive with the substrate, and the inhibition constant (Ki) of it was 1.9 x 10(-8) M.
Aoyagi
T
T
Institute of Microbial Chemistry, Tokyo, Japan.
Yoshida
S
S
Nakamura
Y
Y
Shigihara
Y
Y
Hamada
M
M
Takeuchi
T
T
D000626:Aminopeptidases / Q000037:antagonists & inhibitors*; D000818:Animals; D018826:CD13 Antigens; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003470:Culture Media / Q000032:analysis; D007700:Kinetics; D051379:Mice; D009842:Oligopeptides / Q000302:isolation & purification* / Q000494:pharmacology / Q000633:toxicity; D013302:Streptomyces / Q000032:analysis*
D003470:Culture Media; D009842:Oligopeptides; C063432:probestin; D000626:Aminopeptidases; D018826:CD13 Antigens