A somatostatin-secreting cell line established from a human pancreatic islet cell carcinoma (somatostatinoma): release experiment and immunohistochemical study.
Production and secretion of somatostatin (SRIF) were studied using a carcinoembryonic antigen (CEA)-producing cell line (QGP-1) established from a human pancreatic islet cell carcinoma. High concentrations of SRIF (274 +/- 51 ng/mg of protein, mean +/- SD, n = 5) and CEA (3083 +/- 347 ng/mg of protein, mean +/- SD, n = 5) were present in QGP-1 cells, and the basal secretion rates of SRIF and CEA by the cells (n = 5) were 46.4 +/- 4.8 and 1690 +/- 78 pg/10(5) cells/h, respectively. Immunohistochemical studies revealed the presence of SRIF in xenografts of QGP-1 cells and colocalization of SRIF and CEA. Secretion of SRIF by QGP-1 cells was stimulated in the presence of high K+ (50 mmol) and theophylline (10 mmol), but arginine (10 mmol) and glucose (300 mg/dl) had no effect on the SRIF secretion. The QGP-1 cell line may be useful for studying the regulation mechanism of SRIF secretion.
Iguchi
H
H
Department of Biochemistry, National Kyushu Cancer Center, Fukuoka, Japan.
Hayashi
I
I
Kono
A
A
D007516:Adenoma, Islet Cell / Q000378:metabolism*; D000818:Animals; D002272:Carcinoembryonic Antigen / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D051379:Mice; D008819:Mice, Nude; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D011188:Potassium / Q000494:pharmacology; D013004:Somatostatin / Q000378:metabolism*; D013005:Somatostatinoma / Q000378:metabolism* / Q000473:pathology; D013806:Theophylline / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000378:metabolism
D002272:Carcinoembryonic Antigen; D007328:Insulin; D013004:Somatostatin; D005934:Glucagon; D013806:Theophylline; D011188:Potassium