An IgM specific ELISA for the serodiagnosis of viral bovine respiratory infections.
A capture ELISA for the detection of IgM antibodies to Infectious Bovine Rhinotracheitis (IBR) and to Bovine Respiratory Syncytial (BRS) viruses was developed. In these assays, the first monoclonal antibody to bovine IgM is used as the catching antibody while the second monoclonal detects specific antiviral antibodies. The test was evaluated on serum samples originating from both experimentally and naturally infected animals. From these studies, it has been shown that primary IBR and BRS virus infections can be confirmed using serum samples collected 5-10 days after the appearance of the clinical signs of disease.
Florent
G
G
SmithKline Beecham, Animal Health, Rixensart, Belgium.
Wiseman
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D007242:Herpesvirus 1, Bovine / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000032:analysis* / Q000096:biosynthesis; D007241:Infectious Bovine Rhinotracheitis / Q000175:diagnosis*; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D010253:Respirovirus Infections / Q000175:diagnosis / Q000662:veterinary*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M