title: A study comparing the immunologic responses of swine to pseudorabies viral antigens based on the ELISA, serum virus neutralization, and latex agglutination tests.
abstract: A study of pseudorabies virus (PRV)-vaccinated pigs comparing the immune responses detected by the latex agglutination test (LAT) with responses detected by other routine tests for pseudorabies antibodies indicated that LAT was more sensitive than either the enzyme-linked immunosorbent assay (ELISA) or the serum virus neutralization test (SVNT). The LAT detected antibodies sooner than ELISA and SVNT in unvaccinated pigs after challenge with virulent PRV. The specificities of the 3 tests were found to be near 100%. The LAT is a good alternative to SVNT or ELISA for detection of PRV-specific antibodies.
authors.affiliation: Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011.
authors.forename: M A
authors.initials: MA
authors.lastname: Schoenbaum
authors.forename: G W
authors.initials: GW
authors.lastname: Beran
authors.forename: D P
authors.initials: DP
authors.lastname: Murphy
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D007841:Latex Fixation Tests; D009500:Neutralization Tests; D011237:Predictive Value of Tests; D011557:Pseudorabies / Q000276:immunology*; D011897:Random Allocation; D013552:Swine; D013553:Swine Diseases / Q000276:immunology; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D014765:Viral Vaccines