Expression of the porcine transmissible gastroenteritis coronavirus M protein.
Cloned cDNA encoding the M protein of the porcine transmissible gastroenteritis coronavirus (TGEV) was introduced into a vaccinia virus to examine the function of the amino-terminal signal peptide. The M protein expressed by the recombinant virus was targeted to the Golgi region of infected cells, as is the M protein in cells infected with TGEV. The protein appeared not to undergo processing other than glycosylation. However, the vaccinia-expressed M protein was slightly larger than the protein found in TGEV-infected cells, suggesting that a difference in modification exists between the proteins.
Hogue
B G
BG
Department of Microbiology and Immunology, UCLA School of Medicine 90024.
Nayak
D P
DP
D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D015870:Gene Expression; D005814:Genes, Viral; D008297:Male; D008970:Molecular Weight; D021382:Protein Sorting Signals / Q000235:genetics; D015183:Restriction Mapping; D013552:Swine; D013737:Testis; D005760:Transmissible gastroenteritis virus / Q000235:genetics*; D014616:Vaccinia virus / Q000235:genetics; D014763:Viral Matrix Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification
D021382:Protein Sorting Signals; D014763:Viral Matrix Proteins