title: Seeding of human microvascular endothelial cells onto polytetrafluoroethylene graft material.
abstract: Microvascular endothelial cells from human omental samples were isolated and grown. Adhesion to polytetrafluoroethylene vascular graft material was then studied using scanning electron microscopy and adhesion of cells labelled with indium-111. Grafts coated with fibronectin and type I collagen were found to promote the best adhesion of cells at times up to 90 min. A coating of blood clot matrix was less effective but still resulted in a threefold increase in cell adhesion compared with controls.
authors.affiliation: University Department of Surgery, Royal Free Hospital, London, UK.
authors.forename: G
authors.initials: G
authors.lastname: Stansby
authors.forename: N
authors.initials: N
authors.lastname: Shukla
authors.forename: B
authors.initials: B
authors.lastname: Fuller
authors.forename: G
authors.initials: G
authors.lastname: Hamilton
mesh_terms: D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000637:transplantation* / Q000648:ultrastructure; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009852:Omentum; D011138:Polytetrafluoroethylene
chemical_list: D011138:Polytetrafluoroethylene