Identification of immunodominant polypeptides common between Anaplasma centrale and Anaplasma marginale.
High titered antibody from rabbits immunized with Anaplasma centrale or from cattle recovered from A. centrale infection bound predominantly to several 33-36 kDa polypeptides present in both A. centrale and the Israel-NT isolate of Anaplasma marginale. High titered bovine antibody against the Israel-NT isolate of A. marginale also reacted predominantly with A. centrale polypeptides in this size range. The immunodominance of the 33-36 kDa polypeptides and their cross-reactivity indicate that these shared epitopes may be primarily responsible for the cross-protective immunity between A. centrale and A. marginale.
Shkap
V
V
Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel.
Pipano
E
E
McGuire
T C
TC
Palmer
G H
GH
D000709:Anaplasma / Q000276:immunology*; D000712:Anaplasmosis / Q000276:immunology* / Q000382:microbiology; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D016056:Immunodominant Epitopes / Q000032:analysis*; D008970:Molecular Weight; D011817:Rabbits
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D016056:Immunodominant Epitopes