[Chemiluminescence fiber optic immunosensor for detecting antibodies to the influenza virus].
A new variant of an optoimmunosensor for determination of antibodies to the influenza virus has been elaborated. Its advantages as compared to the traditional solid phase immunoenzyme analysis in respect to sensitivity and expressiveness are demonstrated. Time of the sensor response is below 17 min. When analyzing the 1:320 diluted serum, about 80% of response value is implemented after 6 min. Optimum conditions of the optoimmunosensor transducer regeneration are chosen. They permit reusing it for 20-30 cycles of measurements. A conclusion is made on the prospects of the developed variant of the optosensor for the immunoanalysis of antigens and antibodies under the equilibrium and kinetic conditions.
Arenkov
P Ia
PIa
Berezin
V A
VA
Starodub
N F
NF
D000914:Antibodies, Viral / Q000032:analysis*; D015374:Biosensing Techniques; D005336:Fiber Optic Technology; D006801:Humans; D007124:Immunoenzyme Techniques; D007251:Influenza, Human / Q000175:diagnosis; D049449:Luminescence; D055100:Optical Fibers; D009975:Orthomyxoviridae / Q000276:immunology*; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral
Khemiliuminestsentnyĭ optovolokonnyĭ immunosensor dlia opredeleniia antitel k virusu grippa.