Effect of in vivo T-cell depletion on the effector T-cell function of immunity to Eimeria falciformis.
BALB/c mice were exposed to the enteric parasite Eimeria falciformis to produce a natural acquired immunity. The mice were then depleted of their effector T-cell function by in vivo administration of a cytotoxic Thy-1.2 mouse monoclonal antibody. T-cell depletion was demonstrated by a reduction in concanavalin A-induced proliferation of splenic lymphocytes in treated mice compared with that in controls. Twenty-four hours following T-cell depletion, the mice were challenged with 5,000 oocysts of E. falciformis. Daily total oocyst counts were done for each mouse from days 6 to 21 following challenge. Our studies demonstrated that depleting mice of their effector T-cell function following establishment of immunity caused an abrogation of protective immunity to this parasite.
Stiff
M I
MI
Animal Health Discovery Research Department, Lilly Research Laboratories, Eli Lilly and Company, Greenfield, Indiana 46140.
Vasilakos
J P
JP
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D003048:Coccidiosis / Q000276:immunology*; D004539:Eimeria; D007111:Immunity, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*; D018800:Thy-1 Antigens
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D018800:Thy-1 Antigens