Sensitivity of bacteria to NaNO2 and to L-arginine-dependent system in murine macrophages.
Sensitivity of bacteria to NO2-/NO and to L-arginine-dependent system in murine macrophages was tested. The growth of all bacteria tested, Salmonella typhimurium, Pseudomonas aeruginosa and Staphylococcus epidermidis, was significantly inhibited by NaNO2 at the concentration of 5 to 10 mM. However, L-arginine-dependent effector mechanism of LPS-activated murine macrophages was ineffective in killing these bacteria. It seems that the concentration of NO2-/NO in phagolysosome in the activated macrophages is insufficient to kill the bacteria in situ. We concluded that L-arginine-dependent effector mechanism can hardly work on bacteria killing in activated macrophages.
Saito
S
S
Department of Microbiology, Jichi Medical School, Tochigi.
Onozuka
K
K
Shinomiya
H
H
Nakano
M
M
D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D001433:Bacteriolysis / Q000502:physiology*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D009569:Nitric Oxide / Q000494:pharmacology; D009609:Nitrous Oxide / Q000494:pharmacology; D010587:Phagocytosis; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000276:immunology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000276:immunology; D012977:Sodium Nitrite / Q000494:pharmacology*; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000276:immunology
D008070:Lipopolysaccharides; D009569:Nitric Oxide; D001120:Arginine; D009609:Nitrous Oxide; D012977:Sodium Nitrite