Isolation-induced changes in radioligand binding to benzodiazepine binding sites.
Binding of [3H]flunitrazepam was studied in brain tissues of isolated Wistar rats and compared to group-reared animals. Modifications were observed in hippocampus and cortex (Kd increased) and in cerebellum (Bmax decreased) and when brain sections of control rats were incubated in the bath fluid that had served to incubate sections from isolated rats, a flattening of the saturation curve was observed. Results are discussed in terms of possible modulators of benzodiazepine binding sites, mainly tissue GABA concentrations.
Miachon
S
S
INSERM U 171-CNRS UA 95, Centre Hospitalier Lyon Sud, Pierre Benite France.
Manchon
M
M
Fromentin
J R
JR
Buda
M
M
D000818:Animals; D001345:Autoradiography; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D005971:Glutamates / Q000032:analysis; D018698:Glutamic Acid; D005973:Glutamine / Q000032:analysis; D006624:Hippocampus / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008954:Models, Biological; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000378:metabolism*; D012934:Social Isolation / Q000523:psychology; D012984:Software; D005680:gamma-Aminobutyric Acid / Q000032:analysis
D005971:Glutamates; D011963:Receptors, GABA-A; D005973:Glutamine; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid