Mechanistic studies on DNA photolyase, II. Is the blue enzyme isolated from Escherichia coli a mutant?
The contrasting properties of the DNA photolyases isolated from Escherichia coli, and from Saccharomyces cerevisiae suggested the possibility that the E. coli enzyme may have suffered mutagenesis as a consequence of the extensive use of ultraviolet irradiation/photoreactivation as a selection technique during the cloning. We have therefore recloned this gene using a UV-independent protocol and confirmed the original sequence.
Begley
T P
TP
Department of Chemistry, Cornell University, Ithaca, NY 14853.
D003001:Cloning, Molecular; D003116:Color; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000235:genetics* / Q000528:radiation effects; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D009154:Mutation; D014466:Ultraviolet Rays
D004255:Deoxyribodipyrimidine Photo-Lyase