[Evaluation of 2,5-oligoadenylate synthetase and protein kinase activities in clinical studies of larifan and recombinant interferon alpha in volunteers].
The activity of the interferon-dependent enzymes: 2',5'-oligoadenylate synthetase and protein kinase was determined in blood specimens of volunteers in the clinical trials on reaferon (recombinant alpha 2-interferon) and larifan (replicate RNA of phage f2). It was shown that the preparations increased the activity of 2',5'-oligoadenylate synthetase in the lymphocytes and protein kinase in the plasma of 60 to 70 per cent of the volunteers. The increase in the 2',5'-oligoadenylate synthetase activity did not always correlate with the increase in the interferon content in the serum and was sometimes observed in the absence of the interferon. Marked individual variations in the activity of the enzymes were detected in the volunteers before and after administration of the preparations. The plasma kinase activated by reaferon and larifan phosphorylated proteins with molecular weights of 72 and 30 kD and histones. The effect of reaferon and larifan on the lymphocyte protein kinase activity was determined for the first time. There was a decrease in the enzyme activity under the effect of reaferon which increased after its repeated injections. Unlike the effect of larifan, the inhibitory effect of reaferon was transient. Afterwards, it appeared to be accompanied by a significant increase in the activity of protein kinase in 70 per cent of the volunteers. The dynamics of the changes in the activity of the plasma and lymphocyte protein kinases did not coincide.
Sokolova
T M
TM
Nosik
N N
NN
Lopatina
O A
OA
Krylov
V F
VF
D015088:2',5'-Oligoadenylate Synthetase / Q000097:blood*; D004341:Drug Evaluation; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007369:Interferon Inducers / Q000008:administration & dosage / Q000494:pharmacology*; D007370:Interferon Type I / Q000008:administration & dosage / Q000494:pharmacology*; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D008954:Models, Biological; D009930:Organic Chemicals; D011494:Protein Kinases / Q000097:blood*; D011994:Recombinant Proteins
D007369:Interferon Inducers; D007370:Interferon Type I; D009930:Organic Chemicals; D011994:Recombinant Proteins; C066530:larifan; D011494:Protein Kinases; D015088:2',5'-Oligoadenylate Synthetase
Otsenka aktivnosti 2,5-oligoadenilatsintetazy i proteinkinazy pri klinicheskikh ispytaniiakh larifana i rekombinantnogo alfa2 - interferona na dobrovol'tsakh.