Effects of culture media on spontaneous incidence of mitotic index, chromosomal aberrations, micronucleus counts, sister chromatid exchanges and cell cycle kinetics in peripheral blood lymphocytes of male and female donors.
The spontaneous incidence of mitotic index (MI), chromosomal aberrations (CA), micronucleus counts (MNC), sister chromatid exchanges (SCE) and cell cycle kinetics (CCK) were studied in human peripheral blood lymphocytes grown in M199 and RPMI-1640 culture media. Lower frequencies of CAs, MNC and SCEs were observed in lymphocytes cultured in medium RPMI-1640. The reduction of the MI and the replicative index in M199 medium showed delayed cell cycle kinetics.
Ghosh
B B
BB
Department of Botany, University of Calcutta, India.
Talukder
G
G
Sharma
A
A
D000293:Adolescent; D000328:Adult; D002453:Cell Cycle; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003470:Culture Media / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D008940:Mitotic Index; D012854:Sister Chromatid Exchange
D003470:Culture Media