Biphasic regulation of proopiomelanocortin messenger RNA concentrations by corticotropin-releasing factor in primary cultures of rat hypothalamic neurons.
Primary cultures of rat hypothalamic neurons in which a 1.1-kb proopiomelanocortin (POMC) mRNA can be detected, were incubated with corticotropin-releasing factor (CRF) for periods of up to 84 h. A cyclic modulation of POMC mRNA concentrations by CRF was noted. A rapid acute inhibition of POMC mRNA levels after 1 h of exposure was followed by a transient stimulation and then by a more prolonged inhibition which reached a nadir after 48 h.
L'Héreault
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Molecular Psychogenetics Laboratory, CHUL Research Centre, Ste Foy, Qué., Canada.
Barden
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N
D000818:Animals; D002478:Cells, Cultured; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D004723:Endorphins / Q000378:metabolism; D005260:Female; D007031:Hypothalamus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015151:Immunoblotting; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D011333:Pro-Opiomelanocortin / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D004723:Endorphins; D012333:RNA, Messenger; D011333:Pro-Opiomelanocortin; D003346:Corticotropin-Releasing Hormone