title: Measurement of malondialdehyde in plasma by high performance liquid chromatography with fluorimetric detection.
abstract: The thiobarbituric acid test for malondialdehyde is widely used as an indicator of lipid peroxidation and free radical activity. We describe a simple and sensitive high performance liquid chromatographic (HPLC) method for measuring the malondialdehyde-thiobarbituric acid adduct using fluorimetric detection. Extraction into organic solvent or other complex sample preparation is not required and the assay is sensitive to less than 0.05 mumol/L. This method could easily be applied to any setting where a measure of lipid peroxidation is required.
authors.affiliation: Department of Clinical Biochemistry, Queen's University of Belfast, UK.
authors.forename: I S
authors.initials: IS
authors.lastname: Young
authors.forename: E R
authors.initials: ER
authors.lastname: Trimble
mesh_terms: D001793:Blood Preservation; D001800:Blood Specimen Collection; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005470:Fluorometry; D006801:Humans; D008315:Malondialdehyde / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature; D013858:Thiobarbiturates
chemical_list: D013858:Thiobarbiturates; D008315:Malondialdehyde; C029684:thiobarbituric acid