title: Analysis of the oligosaccharides on androgen-binding proteins: implications concerning their role in structure/function relationships.
abstract: Rat androgen-binding protein (rABP), human testosterone-binding globulin (hTeBG) and rabbit (rb) TeBG are heterodimeric proteins. The source of the heterogeneity arises from the differential glycosylation of a common protein core. This glycosylation results in a heavy subunit (more glycosylation) and a light subunit (less glycosylation). Glycosylation is one factor responsible for multiple charged species seen when rABP, hTeBG, and rbTeBG are analyzed by two-dimensional gel electrophoresis. Enzymatic digestion with the endoglycosidase, peptide: N-glycosidase F indicated that all three proteins have asparagine (Asn)-linked oligosaccharides as their major glycan substituent. Treatment with exoglycosidases provided evidence for terminal sialic acid, galactose and mannose and N-acetylglucosamine residues. About 16-22% of the mass of the heavy subunit and about 8-14% of the mass of the light subunit is contributed by carbohydrate. Serial lectin chromatography indicated that rABP is glycosylated differently from hTeBG and rbTeBG. About 40% of the rABP contains tri and tetraantennary complex oligosaccharides, while only about 20% of the hTeBG and TeBG from pregnant rabbits contains these types of glycans. About 9% of the TeBG from male rabbits bears these types of oligosaccharides. All of the biantennary complex oligosaccharides on rABP are fucosylated on the chitobiose core, but only 8% of those on hTeBG and none of those on rbTeBG are fucosylated in this manner. All three proteins are glycosylated at more than one site. The data indicate that the proteins may have more than one type of oligosaccharide on them. It is likely that differences in glycosylation are responsible for different physiological roles of the proteins.
authors.affiliation: Department of Obstetrics and Gynecology, Vanderbilt University, Nashville, TN 37232-2633.
authors.forename: B J
authors.initials: BJ
authors.lastname: Danzo
authors.forename: J H
authors.initials: JH
authors.lastname: Black
authors.forename: B W
authors.initials: BW
authors.lastname: Bell
mesh_terms: D000727:Androgen-Binding Protein / Q000737:chemistry*; D000818:Animals; D002240:Carbohydrate Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D006023:Glycoproteins / Q000737:chemistry; D006801:Humans; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D012738:Sex Hormone-Binding Globulin / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D000727:Androgen-Binding Protein; D006023:Glycoproteins; D009844:Oligosaccharides; D012738:Sex Hormone-Binding Globulin