[The efficiency of an original selective medium in isolating and identifying gram-negative bacteria in urinary tract infections].
The authors examined 449 samples of urine, bacteriologically important for the minor infection, collected from the patients with clinical forms of urinary infection. Using a selective medium--CMV, enteric agar (Romanian patent--9345/1989), 567 strains of gram-negative germs, chemically belonging to group I (Enterobacteria)-475/83.77%, or to groups II-VI, 66/11.64% were isolated. Other 20 strains, 4.69% could not be identified. Of the total of samples, 333/74.16% were with monobacterial flora versus 116/25.83% samples with pluribacterial flora referring to Enterobacteria associated among them--64/55.17% or to Enterobacteria associated to other gram-negative germs--33/30.17%. Likewise, double or multiple associations among the germs outside the Enterobacteriaceae family were found. The results showed the etiologic participation of 22 bacterial species to which other nonidentified gram-negative microorganisms were added. Our data, more comprehensive than the mentions of the other authors show the efficiency of the culture medium experimented. It has the following advantages: the isolated colonies have a wide chromatic and structural range, showing, in fact, the reactions between the bacterial enzymes and the substances belonging to the medium;--it identifies directly, in the isolation slides, some frequently met species;--it guides sensibly the pricking out; it permits the establishment of the proportion among the types of colonies.
Marica
D
D
Facultatea de medicină veterinară, Cluj-Napoca.
Muntean
E
E
Albu
S
S
Cernatoni
O
O
D001437:Bacteriuria / Q000382:microbiology; D003470:Culture Media; D006090:Gram-Negative Bacteria / Q000145:classification / Q000302:isolation & purification*; D006801:Humans; D014551:Urinary Tract / Q000382:microbiology*; D014552:Urinary Tract Infections / Q000382:microbiology*
D003470:Culture Media
Eficienţa unui mediu selectiv original în izolarea şi identificarea bacteriilor gramnegative din infecţiile tractului urinar.