Map of chartreusin and elsamicin binding sites on DNA.
Three DNA restriction fragments designated tyrT, 102-mer and 70-mer, have been used as substrates for footprinting studies using DNase I in the presence of the structurally similar antibiotics chartreusin and elsamicin A. The sequence-selective binding sites of the antibiotics can be mapped in regions which are rich in guanine + cytosine. Chartreusin and elsamicin appear to recognize and bind preferentially to sequences containing a CpG step. Regions containing a TpG step also seem to be a good binding site. The binding of elsamicin to these sites appears to be more concentration-dependent. A comparative analysis is performed of the sizes and locations of the different binding sites, aimed to infer whether the different biological effects of chartreusin and elsamicin A can be correlated to differences in their sequence-selective binding to DNA.
Salas
X
X
Departamento de Bioquimica y Fisiologia, Universidad de Barcelona, Spain.
Portugal
J
J
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001483:Base Sequence; D001578:Benzopyrans; D001665:Binding Sites; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D016172:DNA Fingerprinting; D006027:Glycosides; D008969:Molecular Sequence Data; D015183:Restriction Mapping
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D001578:Benzopyrans; D006027:Glycosides; D004247:DNA; C002185:chartreusin; C049904:elsamicin A