Establishment of a germ-line competent C57BL/6 embryonic stem cell line.
Embryonic stem (ES) cell lines have been derived from blastocysts of the inbred mouse strain C57BL/6. The highest frequencies of ES cell colonies were observed when blastocysts were explanted directly onto growth-arrested feeder layers of 5637 human bladder carcinoma cells in the presence of conditioned medium. One of the male ES cell lines tested (BL/6-III) was shown to be karyotypically stable and germ-line competent when introduced into BALB/c host blastocysts. These results demonstrate that ES cell lines from inbred mouse strains other than 129/Sv may be used as vectors to introduce selected mutations into the germ-line of mice.
Ledermann
B
B
Preclinical Research, Sandoz Pharma Ltd., Basel, Switzerland.
Bürki
K
K
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D002678:Chimera; D002869:Chromosome Aberrations; D046508:Culture Techniques / Q000379:methods; D004622:Embryo, Mammalian; D005260:Female; D005838:Genotype; D007621:Karyotyping; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D009154:Mutation; D011003:Ploidies; D013234:Stem Cells / Q000166:cytology*; D014998:Y Chromosome