Structure and chromosomal mapping of a highly polymorphic repetitive DNA sequence from the pseudoautosomal region of the mouse sex chromosomes.
The pseudoautosomal region of the Mov15 mouse strain is marked by a Moloney murine leukemia provirus. The sequences flanking the Mov15 provirus were molecularly cloned and shown to consist of a tandemly repeated sequence of 31 nucleotides. Copy number variation of this repeat most likely accounts for the polymorphism in the mouse pseudoautosomal region detected with a probe from the flanking sequences. In situ hybridization to metaphase chromosomes showed heavy labeling of the pairing region of the X and Y chromosomes. The repetitive sequence was also found at the subtelomeric region of three autosomes. A similar level of amplification as the one seen on the sex chromosomes seems to be present on chromosomes 9 and 13. Lower copy number appear to be present on chromosome 4.
Harbers
K
K
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg FRG.
Francke
U
U
Soriano
P
P
Jaenisch
R
R
Müller
U
U
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA; D004251:DNA Transposable Elements; D005787:Gene Frequency; D051379:Mice / Q000235:genetics*; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012730:Sex Chromosomes / Q000648:ultrastructure*
D004251:DNA Transposable Elements; D004247:DNA