Squamous cell carcinoma antigen is a new member of the serine protease inhibitors.
We have cloned cDNA of squamous cell carcinoma antigen. Sequence analysis of the complete 1711 basepairs SCC antigen cDNA revealed that it coded 390 amino acids and no typical signal sequence in the NH2-terminus. Northern blot analysis of human squamous cell poly(A)+ RNA using this cDNA insert as the probe showed a single RNA species of about 1.7 kilobases. The cDNA was expressed in Escherichia coli and the product was detected by immunological methods using antibodies against SCC antigen, indicating that this cDNA encodes the entire SCC antigen sequence. The amino acid homology search revealed that SCC antigen was a member of the serine protease inhibitors family.
Suminami
Y
Y
Division of Obstetrics and Gynecology, Onoda Municipal Hospital, Yamaguchi, Japan.
Kishi
F
F
Sekiguchi
K
K
Kato
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000556:secondary; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D015842:Serine Proteinase Inhibitors / Q000235:genetics*; D015843:Serpins / Q000235:genetics*; D014594:Uterine Neoplasms / Q000235:genetics
D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D015842:Serine Proteinase Inhibitors; D015843:Serpins; C051072:squamous cell carcinoma-related antigen