title: New ELISA test for detection of bovine leukemia virus infections in cattle, using bacterially synthesized p24.
abstract: A new ELISA test is described for the detection of antibodies to bovine leukemia virus protein p24. This test employs a bacterially synthesized p24 antigen which represents a hybrid protein consisting of beta-galactosidase and about 70% of the mature viral p24. The antigen preparation was enriched from Escherichia coli cells to 95% purity and was used for the detection of antibodies in cattle. In a selected set of 100 positive field sera, 97 could be verified by the new test.
authors.affiliation: Department of Cell Differentiation, Academy of Sciences, Berlin-Buch.
authors.forename: H
authors.initials: H
authors.lastname: Siakkou
authors.forename: D
authors.initials: D
authors.lastname: Kube
authors.forename: H
authors.initials: H
authors.lastname: Peters
authors.forename: A
authors.initials: A
authors.lastname: Otto
authors.forename: R
authors.initials: R
authors.lastname: Ulrich
authors.forename: S
authors.initials: S
authors.lastname: Rosenthal
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D007938:Leukemia / Q000175:diagnosis / Q000662:veterinary*; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins