Formation of vesiculated large bodies of Staphylococcus aureus L-form in a liquid medium.
A method is described in which cells of Staphylococcus aureus can be converted to vesiculated large bodies of L-form. When coccal cells were incubated in a liquid growth medium containing D-cycloserine, N-acetylmuramidase and subtilisin, a large number of vesiculated large bodies were formed. Electron microscopy revealed that development of internal vesicles arose after 6 hr of incubation.. When growth inhibitory concentrations of rifampicin, novobiocin, or chloramphenicol were added to the culture at 6 hr of incubation, small-sized nonvesiculated bodies were produced instead of vesiculated forms. The viability of cultures was reduced by rifampicin and novobiocin but not by chloramphenicol.
Yabu
K
K
Department of Biology, Hokuriku University, Ishikawa.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002455:Cell Division; D015169:Colony Count, Microbial; D003470:Culture Media; D003523:Cycloserine / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000494:pharmacology; D007740:L Forms / Q000254:growth & development*; D008858:Microscopy, Phase-Contrast; D013211:Staphylococcus aureus / Q000254:growth & development*; D013381:Subtilisins / Q000494:pharmacology; D014617:Vacuoles / Q000502:physiology* / Q000648:ultrastructure
D000900:Anti-Bacterial Agents; D003470:Culture Media; D003523:Cycloserine; D006026:Glycoside Hydrolases; C026255:endo-N-acetylmuramidase; D013381:Subtilisins