Rapid and simple isolation of multigram goat IgG from serum using Avid AL and radial flow column.
Multigram quantities (2.5-10 g) of highly purified IgG were obtained within 4 h from serum by using Avid AL packed in a radial-flow column. Avid AL is an affinity gel containing a synthetic, low-mol-wt ligand capable of selectively binding IgG from serum of all animal species tested. By packing the gel in a radial-flow column up to 500 mL, a high flow rate of 50 mL/min can be achieved without adversely affecting the performance of the gel and the purity of the isolated antibody.
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BioProbe International Inc., Tustin, CA 92680.
Khatter
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D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats / Q000097:blood / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification*; D013045:Species Specificity
D007074:Immunoglobulin G