title: A combined physical and genetic map of Pseudomonas aeruginosa PAO.
abstract: A combined physical and genetic map of Pseudomonas aeruginosa PAO was constructed by pulsed-field gel electrophoresis and Southern hybridization using cosmid clones from a genomic library carrying known genes. A total of 37 SpeI restriction fragments have been mapped on the 5862 kb genome, and fragment contiguity demonstrated by hybridization with clones from a SpeI junction fragment library and fragments obtained by partial SpeI digestion, both derived from the P. aeruginosa PAO chromosome.
authors.affiliation: Department of Genetics and Developmental Biology, Monash University, Clayton, Victoria, Australia.
authors.forename: E
authors.initials: E
authors.lastname: Ratnaningsih
authors.forename: S
authors.initials: S
authors.lastname: Dharmsthiti
authors.forename: V
authors.initials: V
authors.lastname: Krishnapillai
authors.forename: A
authors.initials: A
authors.lastname: Morgan
authors.forename: M
authors.initials: M
authors.lastname: Sinclair
authors.forename: B W
authors.initials: BW
authors.lastname: Holloway
mesh_terms: D015139:Blotting, Southern; D003001:Cloning, Molecular; D003360:Cosmids; D004251:DNA Transposable Elements; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D015698:Genomic Library; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D004251:DNA Transposable Elements; C056436:endodeoxyribonuclease SpeI; D015252:Deoxyribonucleases, Type II Site-Specific