title: Purification and initial characterisation of koala immunoglobulins.
abstract: The production of koala immunoglobulins (Ig) was elicited by the immunisation of a koala (Phascolarctos cinereus) with bovine serum albumin. This Ig was then purified using the highly specific techniques of affinity chromatography. The purified protein was compared with a "potential" koala Ig protein which was subsequently purified by Protein G chromatography and both proteins were further characterised by agarose/SDS-PAGE and immunoelectrophoresis. Results indicate that koalas do produce Ig in response to antigen challenge, koala Ig has a higher net negative charge than that seen in most other mammals and possibly two subclasses of IgG and IgM are present in normal koala serum.
authors.affiliation: Central Veterinary Laboratories (VETLAB), Department of Agriculture, Adelaide, S.A. Australia.
authors.forename: R
authors.initials: R
authors.lastname: Wilkinson
authors.forename: M
authors.initials: M
authors.lastname: Allanson
authors.forename: V
authors.initials: V
authors.lastname: Kolega
authors.forename: D
authors.initials: D
authors.lastname: Lawrence
authors.forename: S
authors.initials: S
authors.lastname: Neville
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000302:isolation & purification*; D008297:Male; D008394:Marsupialia / Q000276:immunology*; D011817:Rabbits
chemical_list: D007074:Immunoglobulin G