[Cloning the alpha-amylase gene of Streptococcus bovis and its expression in Bacillus subtilis cells].
The gene coding for alpha-amylase from the ruminant bacterium Streptococcus bovis was cloned on the plasmid pMX39 in Bacillus subtilis cells. An alpha-amylase positive colony was isolated in the initial screening of 3900 colonies on the medium containing insoluble starch. The size of the insert was approximately 2.8 kb. The recombinant plasmid was stably maintained in Bacillus subtilis cells under the nonselective conditions.
Iakorski
P
P
Kuntsova
M M
MM
Loseva
E F
EF
Khasanov
F K
FK
D001412:Bacillus subtilis / Q000201:enzymology*; D015139:Blotting, Southern; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D015870:Gene Expression; D010957:Plasmids; D016985:Streptococcus bovis / Q000378:metabolism*; D013696:Temperature; D000516:alpha-Amylases / Q000235:genetics* / Q000378:metabolism
D000516:alpha-Amylases
Klonirovanie gena alpha-amilazy Streptococcus bovis i ego ékspressiia v kletkakh Bacillus subtilis.