Characteristics of mutagenesis by glyoxal in Salmonella typhimurium: contribution of singlet oxygen.
The characteristics of mutagenesis by glyoxal in Salmonella tester strains TA100 and TA104, and particularly a possible role of active oxygen species, were investigated. Glyoxal was converted into a non-mutagenic chemical with glutathione (GSH) by glyoxalase I, and the mutagenic activity was enhanced by the depletion of intracellular GSH. Glyoxal caused the reduction of nitro blue tetrazolium, which was suppressed by the addition of 2,5-diphenylfuran, superoxide dismutase (SOD) and catalase (CAT), scavengers of singlet oxygen (1O2), superoxide radical (O2-) and hydrogen peroxide (H2O2), respectively. However, only the 1O2 scavenger almost completely suppressed the mutagenic activity of glyoxal. Mutagenicity assays using strains pretreated with N,N-diethyldithiocarbamate of a SOD inhibitor and strains with low levels of SOD and CAT indicated that the mutagenesis by glyoxal was independent of intracellular levels of SOD and CAT, though glyoxal itself repressed them. Therefore, all the results suggest that 1O2 formed from glyoxal is related to its mutagenesis, but that neither O2- nor H2O2 is intracellularly predominantly related to it. The action of glyoxal against SOD and CAT, and the formation of glyoxal adducts with amino acids as their components are also discussed.
Ueno
H
H
Division of Environmental Health, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Nakamuro
K
K
Sayato
Y
Y
Okada
S
S
D000596:Amino Acids / Q000378:metabolism; D002374:Catalase / Q000037:antagonists & inhibitors / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006037:Glyoxal / Q000633:toxicity*; D006861:Hydrogen Peroxide / Q000378:metabolism; D016296:Mutagenesis / Q000187:drug effects*; D009152:Mutagenicity Tests; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000187:drug effects; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000378:metabolism
D000596:Amino Acids; D006037:Glyoxal; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D010100:Oxygen