Identification of 11-cis and all-trans-retinal in the photoreceptive organelle of a flagellate green alga.
Isolation of intact photoreceptive organelles (eyespot apparatuses) involved in blue-light mediated photoresponses in a flagellate green alga (Spermatozopsis similis) allowed for the first time the identification of both 11-cis- and all-trans-retinal in a plant cell. Both isomers were identified by HPLC analysis in conjunction with UV spectra. Additionally, reconstitution of a distinct absorption band, centered around 540 nm, was achieved by addition of exogenous 9-cis-retinal to bleached, isolated eyespot apparatuses.
Kreimer
G
G
Universität zu Köln, Botanisches Institut, Germany.
Marner
F J
FJ
Brohsonn
U
U
Melkonian
M
M
D002352:Carrier Proteins / Q000737:chemistry*; D000460:Chlorophyta / Q000737:chemistry* / Q000502:physiology; D005407:Flagella; D015388:Organelles / Q000737:chemistry* / Q000502:physiology; D010786:Photoreceptor Cells / Q000737:chemistry* / Q000502:physiology; D010788:Photosynthesis / Q000502:physiology; D012172:Retinaldehyde / Q000737:chemistry* / Q000502:physiology
C020932:11-cis-retinal-binding protein; D002352:Carrier Proteins; D012172:Retinaldehyde