Evidence for projections from the rostral medullary raphe onto medullary catecholamine neurons in the rat.
Following the iontophoretic deposition of Phaseolus vulgaris leucoagglutinin (PHA-L) into the rostral medullary raphe, which included portions of the caudal nucleus raphe magnus, rostral nucleus raphe pallidus, rostral nucleus raphe obscurus and rostral nucleus reticularis paragigantocellularis, two-color immunoperoxidase staining was employed to demonstrate contiguity between PHA-L-immunoreactive (PHA-LI) varicose fibers and boutons and medullary catecholamine (CA) cells. Raphe projections were contiguous with phenylethanolamine N-methyltransferase-immunoreactive (PNMTI) neurons in the C1, C2 and C3 cell groups and with tyrosine hydroxylase-immunoreactive (THI) neurons in the A1 and A2 cell groups. Contiguity between PHA-LI processes and medullary CA cells was observed most frequently in the C1 cell group. Preliminary findings of this study have been presented previously.
Nicholas
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AP
Department of Anatomy and Neurosciences, University of Texas Medical Branch, Galveston 77550.
Hancock
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MB
D000818:Animals; D002395:Catecholamines / Q000378:metabolism* / Q000502:physiology; D008297:Male; D008526:Medulla Oblongata / Q000166:cytology* / Q000378:metabolism; D009434:Neural Pathways / Q000033:anatomy & histology; D010625:Phenylethanolamine N-Methyltransferase / Q000378:metabolism / Q000502:physiology; D010835:Phytohemagglutinins; D011903:Raphe Nuclei / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000166:cytology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism / Q000502:physiology
D002395:Catecholamines; D010835:Phytohemagglutinins; C100156:leukoagglutinins, plants; D014446:Tyrosine 3-Monooxygenase; D010625:Phenylethanolamine N-Methyltransferase