title: A new qualitative method for detecting IgD in unconcentrated cerebrospinal fluid.
abstract: We report a method which is capable of demonstrating the isoelectric focusing (IEF) pattern of immunoglobulin D in unconcentrated cerebrospinal fluid (CSF) samples containing as little as 0.1-0.5 ng of total IgD. The method used was an immuno-sandwich technique, with alkaline phosphatase enzyme amplification. Oligoclonal and polyclonal IgD patterns were seen in CSF samples. No cross-reactivity with other immunoglobulins (IgG, IgA and IgM) was detected.
authors.affiliation: Department of Special Chemical Pathology, National Hospital for Neurology and Neurosurgery, London, U.K.
authors.forename: M
authors.initials: M
authors.lastname: Mavra
authors.forename: R
authors.initials: R
authors.lastname: Luxton
authors.forename: G
authors.initials: G
authors.lastname: Keir
authors.forename: V
authors.initials: V
authors.lastname: Miletic
authors.forename: E J
authors.initials: EJ
authors.lastname: Thompson
mesh_terms: D006801:Humans; D006863:Hydrogen-Ion Concentration; D007072:Immunoglobulin D / Q000134:cerebrospinal fluid*; D007525:Isoelectric Focusing
chemical_list: D007072:Immunoglobulin D