title: Uracil-DNA glycosylase affects mismatch repair efficiency in transformation and bisulfite-induced mutagenesis in Streptococcus pneumoniae.
abstract: The generalized mismatch repair system of Streptococcus pneumoniae (the Hex system) can eliminate base pair mismatches arising in heteroduplex DNA during transformation or by DNA polymerase errors during replication. Mismatch repair is most likely initiated at nicks or gaps. The present work was started to examine the hypothesis that strand discontinuities arising after removal of uracil by uracil DNA-glycosylase (Ung) can be utilised as strand discrimination signals. We show that mismatch repair efficiency is enhanced 3- to 6-fold when using uracil-containing DNA as donor in transformation. In order to assess the contribution of Ung to nascent strand discrimination for postreplication mismatch repair, we developed a positive selection procedure to isolate S. pneumoniae Ung- mutants. We succeeded in isolating Ung- mutants using this procedure based on chromosomal integration of uracil-containing hybrid DNA molecules. Cloning and characterization of the ung gene was achieved. Comparison of spontaneous mutation rates in strains either proficient or deficient in mismatch and/or uracil repair gave no support to the hypothesis that Ung plays a major role in targeting the Hex system to neosynthesized DNA strands. However Ung activity is responsible for the increased efficiency of mismatch repair observed in transformation with uracil-containing DNA. In addition Ung is involved in repair of bisulfite-treated transforming DNA.
authors.affiliation: Centre de Recherche de Biochimie et de Génétique Cellulaires du CNRS, Université Paul Sabatier, Toulouse, France.
authors.forename: V
authors.initials: V
authors.lastname: Méjean
authors.forename: J C
authors.initials: JC
authors.lastname: Devedjian
authors.forename: I
authors.initials: I
authors.lastname: Rives
authors.forename: G
authors.initials: G
authors.lastname: Alloing
authors.forename: J P
authors.initials: JP
authors.lastname: Claverys
mesh_terms: D003001:Cloning, Molecular; D045647:DNA Glycosylases; D004260:DNA Repair; D004269:DNA, Bacterial; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D008727:Methotrexate / Q000494:pharmacology; D016296:Mutagenesis; D009699:N-Glycosyl Hydrolases / Q000235:genetics / Q000378:metabolism*; D009692:Nucleic Acid Heteroduplexes; D015183:Restriction Mapping; D012293:Rifampin / Q000494:pharmacology; D013296:Streptococcus pneumoniae / Q000201:enzymology / Q000235:genetics*; D013447:Sulfites / Q000494:pharmacology; D014169:Transformation, Bacterial; D051981:Uracil-DNA Glycosidase
chemical_list: D004269:DNA, Bacterial; D009692:Nucleic Acid Heteroduplexes; D013447:Sulfites; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase; C042345:hydrogen sulfite; D012293:Rifampin; D008727:Methotrexate