Cytogenetic analysis of a human familial 15p+ marker chromosome.
An extreme variant of a human 15p+ marker chromosome is described in a human family. The short arm of this variant is greatly enriched in ribosomal RNA genes, and there are up to three secondary constrictions potentially active for rRNA transcription. These sites are hypomethylated, whereas the rest of rDNA is highly methylated, as shown by the isoschizomer pair HpaII-MspI. This chromosome variant is similar to others previously described for chromosomes 14 and 22, but some differences have been found after C-banding and after treatments with AluI and Sau3AI.
Velázquez
M
M
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Visedo
G
G
Ludeña
P
P
de Cabo
S F
SF
Sentís
C
C
Fernández-Piqueras
J
J
D015139:Blotting, Southern; D002478:Cells, Cultured; D002871:Chromosome Banding; D002884:Chromosomes, Human, Pair 15; D015342:DNA Probes / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D010957:Plasmids / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D015183:Restriction Mapping
D015342:DNA Probes; D004275:DNA, Ribosomal; D005819:Genetic Markers; D012337:RNA, Ribosomal, 18S