Localization by in situ hybridization of gamma-glutamyl transpeptidase mRNA in the rat kidney using 35S-labeled RNA probes.
gamma-Glutamyl transpeptidase (GGT) is involved in the extraction of plasma glutathione in the postglomerular compartment of the kidney. The enzyme is distributed in the proximal tubule associated with the apical brush border. Using in situ hybridization with 35S-labeled anti-sense and sense RNA GGT probes on rat kidney cryostat sections, the present study demonstrates that GGT mRNA transcripts are detected in proximal tubules localized in the inner cortex, outer medulla, and medullary rays of the cortex. Such a distribution suggests that the GGT gene is mainly expressed in the more distal part of the proximal tubule, i.e., the pars recta. As a control, in situ hybridization has also been performed using a 35S-labeled beta-actin anti-sense RNA probe showing a diffuse pattern of distribution of beta-actin RNA transcripts particularly in the outer cortex. This highly sensitive method using single strand asymetric RNA probes, with a markedly reduced nonspecific binding is promising for the study of gene expression patterns in the kidney in order to clarify their heterogeneity in the various segments of the nephron.
Lebargy
F
F
Unités INSERM 139 et 99, Département d'Histologie, Faculté de Médecine, Université Paris, Creteil, France.
Bulle
F
F
Siegrist
S
S
Guellaen
G
G
Bernaudin
J F
JF
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D007672:Kidney Cortex / Q000201:enzymology; D007679:Kidney Medulla / Q000201:enzymology; D007687:Kidney Tubules, Proximal / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013460:Sulfur Isotopes; D005723:gamma-Glutamyltransferase / Q000235:genetics*
D015347:RNA Probes; D012333:RNA, Messenger; D013460:Sulfur Isotopes; D005723:gamma-Glutamyltransferase