Kinetics of insulin aggregation in aqueous solutions upon agitation in the presence of hydrophobic surfaces.
The stability of protein-based pharmaceuticals (e.g., insulin) is important for their production, storage, and delivery. To gain an understanding of insulin's aggregation mechanism in aqueous solutions, the effects of agitation rate, interfacial interactions, and insulin concentration on the overall aggregation rate were examined. Ultraviolet absorption spectroscopy, high-performance liquid chromatography, and quasielastic light scattering analyses were used to monitor the aggregation reaction and identify intermediate species. The reaction proceeded in two stages; insulin stability was enhanced at higher concentration. Mathematical modeling of proposed kinetic schemes was employed to identify possible reaction pathways and to explain greater stability at higher insulin concentration.
Sluzky
V
V
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge 02139.
Tamada
J A
JA
Klibanov
A M
AM
Langer
R
R
D000327:Adsorption; D000818:Animals; D002417:Cattle; D066298:In Vitro Techniques; D007328:Insulin / Q000737:chemistry*; D007700:Kinetics; D008956:Models, Chemical; D011138:Polytetrafluoroethylene; D011485:Protein Binding; D011489:Protein Denaturation; D012995:Solubility; D013816:Thermodynamics; D014867:Water
D007328:Insulin; D014867:Water; D011138:Polytetrafluoroethylene