Direct analysis of microbial extracts containing metabolites of ethylenediamine-type antihistamines via high-performance liquid chromatography-thermospray mass spectrometry.
The utility of high-performance liquid chromatography-thermospray mass spectrometry (HPLC-TSMS) for the characterization of the ethylenediamine-type antihistamines, pyrilamine, methapyrilene, tripelennamine, and thenyldiamine, and their methylene chloride-extractable microbial metabolites from a biological matrix is demonstrated. Typically, the [M + H]+ ion was detected as the base peak in the TS mass spectra of these compounds. The ethylenediamine-type antihistamine metabolites were detected in an extract of a fungal culture grown in the presence of 5 mg of the antihistamine. A detection limit of 200 ng was observed for the HPLC-TSMS analysis of pyrilamine.
Korfmacher
W A
WA
National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas 72079-9502.
Getek
T A
TA
Hansen
E B
EB
Cerniglia
C E
CE
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005069:Evaluation Studies as Topic; D006634:Histamine H1 Antagonists / Q000032:analysis* / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D008701:Methapyrilene / Q000032:analysis; D015394:Molecular Structure; D009090:Mucorales / Q000032:analysis; D011725:Pyridines / Q000032:analysis; D011738:Pyrilamine / Q000032:analysis; D014309:Tripelennamine / Q000032:analysis
D006634:Histamine H1 Antagonists; D011725:Pyridines; D014309:Tripelennamine; D008701:Methapyrilene; C005822:thenyldiamine; D011738:Pyrilamine