Images of 16S ribosomal RNA by scanning tunnelling microscopy.
We report the use of scanning tunnelling microscopy (STM) to study surface topographies of complex nucleic acid structures. From low-resolution STM images of uncoated 16S ribosomal RNA, we demonstrate the possibility of determining several objective parameters (molecular mass and radius of gyration) in order to characterize and identify the molecules observed. These parameters were compared with values obtained by other physical methods and the radius of gyration was found to be the most reliable. At high resolution, it was possible to measure the main dimensions of selected V-form particles more precisely than with electron microscopy. Images of the more compact form have been also obtained that show different domains in the macromolecular structure.
Flamion
P J
PJ
Laboratoire de Biophysique, Faculté de Pharmacie, Dijon, France.
Cachia
C
C
Schreiber
J P
JP
David
T
T
Lesniewska
E
E
Goudonnet
J P
JP
D004926:Escherichia coli / Q000235:genetics*; D007091:Image Processing, Computer-Assisted; D016252:Microscopy, Scanning Tunneling; D016012:Poisson Distribution; D011336:Probability; D012336:RNA, Ribosomal, 16S / Q000648:ultrastructure*
D012336:RNA, Ribosomal, 16S