Stress-induced oligomerization and chromosomal relocalization of heat-shock factor.
The induction of heat-shock transcription factor (HSF) binding to DNA is accomplished by a heat-induced oligomerization. The transition to the induced state is accompanied by a chromosomal redistribution of HSF to the heat-shock puff sites. Over 150 additional chromosomal sites also accumulate HSF, including developmental loci that are repressed during heat shock. These findings suggest an unforeseen role for HSF as a repressor of normal gene activity during heat stress.
Westwood
J T
JT
Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
Clos
J
J
Wu
C
C
D000818:Animals; D001665:Binding Sites; D002467:Cell Nucleus / Q000502:physiology; D002875:Chromosomes / Q000502:physiology* / Q000648:ultrastructure; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D004330:Drosophila / Q000235:genetics*; D000076249:Heat Shock Transcription Factors; D006358:Hot Temperature; D046911:Macromolecular Substances; D011485:Protein Binding; D014157:Transcription Factors / Q000378:metabolism*
D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; D046911:Macromolecular Substances; D014157:Transcription Factors; D004247:DNA