Depolymerization of synovial fluid hyaluronic acid (HA) by the complete myeloperoxidase (MPO) system may involve the formation of a HA-MPO ionic complex.
Gel filtration analysis (Sephacryl S-1000) indicated that the Mr of purified equine synovial cell culture 3H-hyaluronic acid (HA) (Mr greater than 1.67 x 10(7) Da) decreased in a concentration dependent manner after exposure to hypochlorite (OCl-). Both high (equine) and medium (human, Mr = 5.5 x 10(5) Da) molecular weight HA were cleaved by the complete myeloperoxidase system (MPO/H2O2/Cl-). Purified human neutrophil myeloperoxidase (MPO) bound tightly to HA-Sepharose and we suggest that this is due to a strong ionic interaction between HA and MPO. The formation of such a complex did not disturb MPO activity. The significance of these results in relation to our previous studies concerning the reduction in viscosity and potential cleavage of HA by the product of the MPO/H2O2/Cl- system is discussed.
Green
S P
SP
Department of Medicine, Melbourne University, John Curtin School of Medical Research, Australian National University, Canberra, ACT.
Baker
M S
MS
Lowther
D A
DA
D002478:Cells, Cultured; D002850:Chromatography, Gel / Q000379:methods; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000032:analysis / Q000378:metabolism*; D006997:Hypochlorous Acid / Q000494:pharmacology; D008970:Molecular Weight; D009195:Peroxidase / Q000032:analysis / Q000378:metabolism*; D011108:Polymers; D013582:Synovial Fluid / Q000737:chemistry*; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism; D014783:Viscosity
D006025:Glycosaminoglycans; D011108:Polymers; D006997:Hypochlorous Acid; D006820:Hyaluronic Acid; D009195:Peroxidase