Standard calibration proteins for Western blotting obtained by genetically prepared protein A conjugates.
Genetically prepared protein A fusion proteins, having retained antibody binding capacity, were used to design different well-defined standard molecular weight marker proteins for Western blotting. The blotted marker proteins are developed at the same time and with the same reagents as the protein sample of interest.
Lindbladh
C
C
Department of Pure and Applied Biochemistry, University of Lund, Sweden.
Mosbach
K
K
Bülow
L
L
D000469:Alkaline Phosphatase / Q000235:genetics / Q000592:standards; D015153:Blotting, Western / Q000592:standards*; D004926:Escherichia coli / Q000235:genetics; D008156:Luciferases / Q000235:genetics / Q000592:standards; D008970:Molecular Weight; D011506:Proteins / Q000235:genetics / Q000592:standards*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000592:standards; D012015:Reference Standards; D013205:Staphylococcal Protein A / Q000737:chemistry / Q000235:genetics*
D011506:Proteins; D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A; D008156:Luciferases; D000469:Alkaline Phosphatase