Effects of chlorpromazine on the cell envelope proteins of Escherichia coli.
Chlorpromazine (CPZ), at a concentration of 60 micrograms/ml of medium completely inhibited the replication of Escherichia coli. At concentrations below this MIC, CPZ caused transient induction of filamentation, such that by the end of 5 h, all of the cells were filaments, and by the end of 24 h, only rod-shaped E. coli were present. The reversion to normal morphology in the presence of CPZ was not due to either the degradation of CPZ or the selection of CPZ-resistant mutants. The electrophoretic pattern of proteins extracted from isolated cell envelopes of CPZ-induced filaments as well as from E. coli that reverted to normal morphology was distinctly different from that of the controls.
Amaral
L
L
Department of Clinical Laboratories, Bronx Lebanon Hospital Center, New York 10457.
Lorian
V
V
D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D002746:Chlorpromazine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004586:Electrophoresis; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D008565:Membrane Proteins / Q000302:isolation & purification*; D013936:Thymidine / Q000378:metabolism
D008565:Membrane Proteins; D002746:Chlorpromazine; D013936:Thymidine