Uptake of beta-hydroxybutyrate in perfused hindquarter of starved and diabetic rats.
To elucidate the peripheral ketone body uptake and the role of insulin in regulating peripheral ketone body utilization in starvation and diabetes mellitus, uptake of beta-hydroxybutyrate (BOHB) was investigated in the perfused hindquarter of starved (72 hour) or streptozotocin-induced (65 mg/kg, intraperitoneally) diabetic rats. Blood concentration of BOHB was significantly higher in diabetic (1,380 +/- 250 mumol/L) and starved (1,229 +/- 245 mumol/L) rats than in controls (104 +/- 8 mumol/L). The hindquarter was perfused with synthetic medium at a flow rate of 0.5 mL/g muscle weight/min. BOHB was added to the medium at a concentration of 0.1, 0.5, 2, or 10 mmol/L, and insulin was added at a concentration of 20, 100, or 500 microU/mL. In the hindquarter perfused with 0.1, 0.5, 2, or 10 mmol/L BOHB, fractional uptake of BOHB in the absence or presence of insulin was significantly lower in diabetic and starved rats than in controls. The addition of 100 or 500 microU/mL insulin significantly increased BOHB uptake in the perfused hindquarter of control rats; however, insulin addition did not significantly increase BOHB uptake in the perfused hindquarter of starved and diabetic rats. These results suggest that BOHB uptake is markedly reduced in the perfused hindquarter of starved and diabetic rats, and that physiological dose of insulin stimulates BOHB uptake in control rats, but not in starved and diabetic rats.
Ikeda
T
T
First Department of Internal Medicine, Tottori University School of Medicine, Yonago, Japan.
Ohtani
I
I
Fujiyama
K
K
Hoshino
T
T
Tanaka
Y
Y
Takeuchi
T
T
Mashiba
H
H
D020155:3-Hydroxybutyric Acid; D000090:Acetoacetates / Q000378:metabolism; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005947:Glucose / Q000493:pharmacokinetics; D006614:Hindlimb; D006885:Hydroxybutyrates / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D007328:Insulin / Q000493:pharmacokinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013217:Starvation / Q000378:metabolism*
D000090:Acetoacetates; D006885:Hydroxybutyrates; D007328:Insulin; C016635:acetoacetic acid; D005947:Glucose; D020155:3-Hydroxybutyric Acid