title: The effect of acute and chronic protein loading on urinary pepsinogen A excretion.
abstract: In 8 healthy male volunteers, urinary excretion (UE) and fractional clearance (FC) of pepsinogen A (PGA), beta 2-microglobulin (beta 2-m) and albumin were measured after 6 days high protein diet (HPD; 2.0 g/kg/day) and compared to values obtained after 6 days low protein diet (LPD; 0.5 g/kg/day). In addition, the effect of an acute protein load (APL; 500 g beef) on these variables were measured. Both chronic and acute protein loading induced a rise in glomerular filtration rate (GFR) of about 10% together with a parallel rise in effective renal plasma flow. UE PGA and FC PGA increased both after HPD (UE PGA 1,707 +/- 1,106 ng/min; FC PGA 23 +/- 12%) as compared to LPD (UE PGA 1,200 +/- 987 ng/min, p less than 0.01; FC PGA 18 +/- 12%, p less than 0.05), and after APL (UE PGA 2,276 +/- 1,389 ng/min; FC PGA 26 +/- 16%) as compared to baseline (UE PGA 1,418 +/- 965 ng/min, p less than 0.02; FC PGA 21 +/- 12%, p less than 0.05). UE and FC of beta 2-m and albumin were not affected by protein loading. As PGA is nearly freely filtered, it is concluded that the increase in fractional PGA clearance reflects a decrease in fractional tubular PGA reabsorption. Our results suggest that an increase in fractional protein clearance after protein loading is not necessarily due to an impaired glomerular permselectivity but represents a decreased fractional tubular reabsorption as a result of a GFR-mediated increase in filtered load without a concomitant increase in tubular reabsorption.
authors.affiliation: Department of Internal Medicine, Free University Hospital, Amsterdam, The Netherlands.
authors.forename: M A
authors.initials: MA
authors.lastname: ten Dam
authors.forename: G J
authors.initials: GJ
authors.lastname: van Kamp
authors.forename: A
authors.initials: A
authors.lastname: Kok
authors.forename: S G
authors.initials: SG
authors.lastname: Meuwissen
authors.forename: A J
authors.initials: AJ
authors.lastname: Donker
authors.forename: R W
authors.initials: RW
authors.lastname: ten Kate
mesh_terms: D000328:Adult; D000419:Albuminuria; D001794:Blood Pressure; D004044:Dietary Proteins / Q000494:pharmacology*; D005919:Glomerular Filtration Rate; D006801:Humans; D008297:Male; D010435:Pepsinogens / Q000652:urine*; D012016:Reference Values; D001613:beta 2-Microglobulin / Q000652:urine*
chemical_list: D004044:Dietary Proteins; D010435:Pepsinogens; D001613:beta 2-Microglobulin