title: Evaluation of a new human immunodeficiency virus (HIV) antibody detection method based on time-resolved fluoroimmunoassay.
abstract: We have developed a time-resolved fluoroimmunoassay for the detection of anti-HIV antibody using purified virus, recombinant gp120 or a synthetic peptide derived from gp41. The results of time-resolved fluoroimmunoassay correlated well with those obtained by enzyme immunoassay and with the results of immunoblotting. This time-resolved fluoroimmunoassay has sufficient sensitivity and specificity for clinical use.
authors.affiliation: Division of AIDS Virus, National Institute of Health, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ushijima
authors.forename: H
authors.initials: H
authors.lastname: Nakate
authors.forename: H
authors.initials: H
authors.lastname: Kohno
authors.forename: K
authors.initials: K
authors.lastname: Okuda
mesh_terms: D015200:Fluoroimmunoassay; D015483:HIV Antibodies / Q000032:analysis*; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D015700:HIV Envelope Protein gp41 / Q000276:immunology; D006801:Humans
chemical_list: D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41