Conversion of biological and immunological properties during a process of decapsulation in a strain of Staphylococcus hyicus.
Repeated subculture at 42 degrees C of Staphylococcus hyicus strain ST67P, which exhibits streaming-type growth in a soft-agar medium, yielded three variants, ST67L, ST67S and ST67C, which had different colonial morphologies; small compact colonies possessing long and short tails and perfect compact colonies. The parent strain and ST67L respectively gave strong and weak positive intensity when stained by rabbit antisera prepared by capsular type I and II strains of Staph. epidermidis conjugated with fluorescein isothiocyanate. Variant ST67L gave a positive result with antiserum prepared by capsular type I strain and no staining was observed with variants ST67S and ST67C against these antisera preparations. Strain ST67C had the lowest virulence although no remarkable difference was shown between the parent strain and variants ST67L and ST67S. The cell volume index of the parent strain was 1.35, 2.43 and 3.71 times larger than those of ST67L, ST67S and ST67C, respectively. The converting activity of rabbit anti-ST67P strain serum absorbed by strain ST67C required four times more of the organisms than strain ST67P, changing the colonial morphology of the strain from diffuse to compact type by the addition of antiserum to soft agar medium. Positive coagulase and false positive clumping factor reaction were shown in variants ST67C, but no remarkable alteration was observed with 19 biochemical properties determined by a conventional identification kit. In ulta-thin sections of the parent strain labelled with rabbit anti-strain ST67P serum conjugated with ferritin, large capsule surrounded by ferritin granules were demonstrated by electron microscopy.(ABSTRACT TRUNCATED AT 250 WORDS)
Yoshida
E
E
Department of Microbiology, St. Marianna University School of Medicine, Kawasaki, Japan.
Ichiman
Y
Y
Suganuma
M
M
Yoshida
K
K
D000818:Animals; D001681:Biological Assay; D005260:Female; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D008854:Microscopy, Electron; D011817:Rabbits; D013210:Staphylococcus / Q000276:immunology / Q000472:pathogenicity / Q000648:ultrastructure*; D014774:Virulence
D007106:Immune Sera