Ubiquitin: preparative chemical synthesis, purification and characterization.
An improved total synthesis of ubiquitin has been achieved by the Fmoc/t-butyl solid-phase methodology using NGPmc protection of the Arg residues. Optimization of the purification protocol has offered the material in substantial quantity (90 mg) in an overall yield of 4.2%. Characterization of the isolated products has opened the way to the studies of the folding pathway and the preparation of structural analogues.
Ogunjobi
O
O
Department of Chemistry, University of Edinburgh, U.K.
Ramage
R
R
D002851:Chromatography, High Pressure Liquid; D007525:Isoelectric Focusing; D008722:Methods; D011487:Protein Conformation; D014452:Ubiquitins / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification
D014452:Ubiquitins