Modified low density lipoproteins activate human macrophages to secrete immunoreactive endothelin.
This study attempted to determine if low density lipoproteins (LDL) induce the production of endothelins (ET) by human macrophages. Non-protected LDL from macrophage induced oxidation (n-LDL), copper-oxidized LDL (Ox-LDL), acetylated-LDL (Ac-LDL), butylated hydroxytoluene-LDL (BHT-LDL), BHT-Ac-LDL, polyinosinic acid (PiA, 1.5 micrograms/ml), phorbol myristate acetate (PMA; 0.5 microM) and BHT alone (20 microM) were studied. The different compounds had the following potency to stimulate the ET secretion: PMA greater than Ox-LDL greater than Ac-LDL greater than n-LDL greater than BHT-LDL greater than PiA greater than PiA + Ac-LDL greater than BHT. In conclusion, modified LDL stimulated ET secretion by human macrophages.
Martin-Nizard
F
F
Department of Lipoproteins and Atherosclerosis Research, Pasteur Institute, Lille, France.
Houssaini
H S
HS
Lestavel-Delattre
S
S
Duriez
P
P
Fruchart
J C
JC
D001665:Binding Sites; D002478:Cells, Cultured; D016232:Endothelins / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D016232:Endothelins; D008077:Lipoproteins, LDL; D013755:Tetradecanoylphorbol Acetate