title: [Occurrence of rotavirus RNA electropherotypes in the pediatric population in Slovakia].
abstract: Using the method of electrophoresis in polyacrylamide gel (PAGE), the authors assessed electropherotypes of rotavirus RNA in extracts of 119 specimens of faeces of sick children and from neonates with asymptomatic rotavirus infection. In both investigated groups they detected electropherotypes of five profiles: of these three had the nature of "long" electropherotypes and were detected in 91.6% of the tested samples. In the group of sick children they recorded circulation of four electropherotypes with exchange of the dominant type of electropherotype after a three-year interval. In the group of healthy neonates they detected in the course of 21 months the same electropherotype, which was not detected in the group of sick children throughout the period of investigation. The identity of RNA profiles of strains from neonates was confirmed by co-electrophoresis.
authors.affiliation: Výskumný ústav preventívneho lekárstva, Bratislava.
authors.forename: J
authors.initials: J
authors.lastname: Tietzová
authors.forename: L
authors.initials: L
authors.lastname: Sefcovicová
authors.forename: M
authors.initials: M
authors.lastname: Barová
mesh_terms: D002648:Child; D003604:Czechoslovakia; D004591:Electrophoresis, Polyacrylamide Gel; D005759:Gastroenteritis / Q000382:microbiology; D006801:Humans; D007231:Infant, Newborn; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D012367:RNA, Viral
vernacular_title: Výskyt RNA elektroforetypov rotavírusov cirkulujúcich v detskej populáchii SSR.