[Penile lesions induced by human papillomavirus. An immunohistochemical study and DNA hybridization].
Forty-nine male patients with clinically evident genital warts or suspected HPV-induced lesions detected by 5% acetic acid test were biopsied. Typical cytological and structural HPV-induced changes were observed on histological examination in 37 of 49 patients. The immunohistochemical detection of HPV capsid antigen was positive in 15 of 45 cases. In situ HPV-DNA hybridization was positive in 26 of 47 cases (24 HPV 6/11; 2 HPV 16/18). Quick blot HPV-DNA hybridization was positive in 20 of 28 cases (17 HPV 6/11 of which one positive for both HPV 6/11 and 31/33/35; 1 HPV 16/18; 2 cases positive for HPV detection, but not typed for HPV 6/11, 16/18, 31/33/35). Characteristics and use of histopathologic, immunohistochemical and HPV-DNA hybridization methods are analyzed and compared.
Caresana
G
G
IRCCS Policlinico San Matteo, Università degli Studi di Pavia.
Tenti
P
P
Di Matteo
A
A
Aguzzi
A
A
Del Forno
C
C
D000956:Antigens, Viral / Q000032:analysis; D002213:Capsid / Q000276:immunology; D003218:Condylomata Acuminata / Q000175:diagnosis*; D015344:DNA Probes, HPV; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D027383:Papillomaviridae / Q000276:immunology; D010412:Penile Neoplasms / Q000175:diagnosis*
D000956:Antigens, Viral; D015344:DNA Probes, HPV
Lesioni peniene da papillomavirus umano. Studio immunoistochimico e con ibridazione del DNA.