Blood-nerve barrier: distribution of anionic sites on the endothelial plasma membrane and basal lamina of dorsal root ganglia.
Previous investigations of the blood-nerve barrier have correlated the greater permeability of ganglionic endoneurial vessels, compared to those of nerve trunks, with the presence of fenestrations and open intercellular junctions. Recent studies have demonstrated reduced endothelial cell surface charge in blood vessels showing greater permeability. To determine the distribution of anionic sites on the plasma membranes and basal laminae of endothelial cells in dorsal root ganglia, cationic colloidal gold and cationic ferritin were used. Electron microscopy revealed the existence of endothelial microdomains with differing labelling densities. Labelling indicated that caveolar and fenestral diaphragms and basal laminae are highly anionic at physiological pH, luminal plasma membranes and endothelial processes are moderately charged and abluminal plasma membranes are weakly anionic. Tracers did not occur in caveolae or cytoplasmic vesicles. In vitro tracer experiments at pH values of 7.3, 5.0, 3.5 and 2.0 indicated that the anionic charge on the various endothelial domains was contributed by chemical groups with differing pKa values. In summary, the labelling of ganglionic and sciatic nerve vessels was similar except for the heavy labelling of diaphragms in a minority of endoneurial vessels in ganglia. This difference is likely to account in part for the greater permeability of ganglionic endoneurial vessels. The results are discussed with regard to the blood-nerve and -brain barriers and vascular permeability in other tissues and a comparison made between the ultrastructure and anionic microdomains of epi-, peri- and endoneurial vessels of dorsal root ganglia and sciatic nerves.
Bush
M S
MS
Reta Lila Weston Institute of Neurological Studies, London, UK.
Reid
A R
AR
Allt
G
G
D000818:Animals; D000838:Anions / Q000032:analysis*; D001485:Basement Membrane / Q000737:chemistry; D002199:Capillary Permeability / Q000502:physiology*; D002462:Cell Membrane / Q000737:chemistry; D004730:Endothelium, Vascular / Q000737:chemistry*; D005727:Ganglia, Spinal / Q000737:chemistry* / Q000648:ultrastructure; D007275:Injections, Intravenous; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000737:chemistry / Q000648:ultrastructure
D000838:Anions