1,2-Diacylglycerol and ceramide levels in rat liver and skeletal muscle in vivo.
1,2-Diacylglycerol and ceramide levels were measured in liver and skeletal muscle of rats under the following four experimental conditions: 1) during rapid fetal growth in the second half of gestation and during postnatal aging, 2) during tissue anoxia lasting up to 10 min, 3) during fasting for up to 6 days, and 4) during stress induced by injection of endotoxin (lipopolysaccharide from Salmonella enteritidis). 1,2-Diacylglycerol and ceramide levels in fetal liver were 77 and 58% lower, respectively, than those of young rats after weaning. 1,2-Diacylglycerol and ceramide concentrations in liver of postnatal rats were not influenced by aging. Anoxia produced a 41-64% increase in liver 1,2-diacylglycerol levels and a 9-21% increase in liver ceramides. Fasting of adult rats for up to 6 days had no effect on 1,2-diacylglycerol and ceramide levels in liver. However, fasting increased skeletal muscle concentrations of 1,2-diacylglycerol and ceramides 88 and 44%, respectively. Injection of endotoxin had a biphasic effect on liver 1,2-diacylglycerol levels, causing a transient 52% increase at 1 h, followed by a 24-38% decrease below the control level at 17 h after endotoxin administration. Liver ceramide levels were increased 66, 52, and 27% at 1, 3, and 5 h after endotoxin, respectively, but did not differ from control at 17 h after injection. Endotoxin had no effect on muscle 1,2-diacylglycerol and ceramide concentrations at any interval.
Turinsky
J
J
Department of Physiology and Cell Biology, Albany Medical College, New York 12208.
Bayly
B P
BP
O'Sullivan
D M
DM
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002518:Ceramides / Q000378:metabolism*; D004075:Diglycerides / Q000378:metabolism*; D004731:Endotoxins / Q000494:pharmacology; D005215:Fasting; D000860:Hypoxia / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012477:Salmonella enteritidis
C011439:1,2-diacylglycerol; D002518:Ceramides; D004075:Diglycerides; D004731:Endotoxins