Structure and polymorphic map of human lipoprotein lipase gene.
Lipoprotein lipase (LPL) catalyzes the key step for the removal of triacylglycerol-rich lipoproteins from the circulation. In this paper, we report the cloning and structure of the normal human LPL gene, which was isolated in three overlapping lambda phage clones that span about 35 kilo bases (kb) of the genetic locus. The peptide coding region of the gene is approx. 23 kb in length and contains nine exons with intron sizes ranging from 0.7 to 8.7 kb. The entire 3' untranslated region is in the tenth exon. Specific sequences in this region support the hypothesis that two mRNA species found for human LPL are generated by differential utilization of polyadenylation signals. The first exon occurs in the 5' untranslated region and the region coding for the signal peptide. The second exon includes the protein domain coding for the N-linked glycosylation site that is required for the expression of enzyme activity. The fourth exon contains the region that was proposed as a lipid binding domain, the sixth for one putative heparin binding domain, and the eighth codes for a domain containing another N-linked glycosylation site. These results suggest that the unique structural and functional domains are confined to specific exons. The PvuII polymorphic site was located within the intron between exon 6 and 7 and the HindIII polymorphic site to the 3' flanking region. The location of these polymorphic sites suggests that the PvuII restriction fragment length polymorphism (RFLP) associated with lipase deficiency in a few Japanese kindred may be a linkage marker for a functional defect of LPL, while the HindIII RFLP associated with hypertriglyceridemia may be important for gene regulation of LPL.
Oka
K
K
Laboratory of Molecular Genetics, Medlantic Research Foundation, Washington, D.C. 20010.
Tkalcevic
G T
GT
Nakano
T
T
Tucker
H
H
Ishimura-Oka
K
K
Brown
W V
WV
D000595:Amino Acid Sequence; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D005091:Exons; D005796:Genes; D006801:Humans; D007438:Introns; D008071:Lipoprotein Lipase / Q000172:deficiency / Q000235:genetics*; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000032:analysis; D015183:Restriction Mapping
D012333:RNA, Messenger; D011061:Poly A; D008071:Lipoprotein Lipase