Results of prenatal alcohol exposure on the dimensions and binucleation of cardiac myocytes in neonatal and weanling rats.
Sprague-Dawley rats were exposed to ethyl alcohol in utero. The effect of chronic prenatal exposure was examined by giving mature females alcohol in isocaloric liquid diets which served as the sole source of liquid and caloric intake before mating and throughout gestation. Controls consisted of females maintained on laboratory chow or an isocaloric liquid diet minus alcohol before and during gestation. The offspring were sacrificed at 21 days of age (weanlings) and the hearts dissociated enzymatically to give purified cardiac myocytes. The effects of daily acute prenatal alcohol exposure were studied by gastric intubation of alcohol to chow-fed females for the duration of pregnancy. The doses used approximated 4 and 5 shots of 80 proof liquor per day by a person weighing 150 lb. These offspring were sacrificed at 2, 6, and 21 days postnatal and cardiac myocytes prepared as above. Heart weights were determined and cardiac myocytes were analyzed for cell length, volume, cross-sectional area, and percent binucleation. Additionally, nuclear DNA content was measured in all of the 21 day offspring. Statistical analysis of the data showed no significant differences between hearts exposed to prenatal alcohol and nonexposed controls with either regimen with the exception of percent binucleation which was significantly but only slightly higher in the 6-day-old hearts. These findings are discussed in relation to anatomical heart defects found in patients with full fetal alcohol syndrome.
Alvarez
M R
MR
Department of Biology, University of South Florida, Tampa 33620-5150.
Clark
L C
LC
Moore
J A
JA
Morales
M C
MC
Gerdes
A M
AM
D000375:Aging; D000818:Animals; D004247:DNA / Q000032:analysis; D004032:Diet; D000431:Ethanol / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D006321:Heart / Q000187:drug effects* / Q000254:growth & development; D008431:Maternal-Fetal Exchange; D009206:Myocardium / Q000166:cytology* / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D000431:Ethanol; D004247:DNA