Purification and initial characterisation of koala immunoglobulins.
The production of koala immunoglobulins (Ig) was elicited by the immunisation of a koala (Phascolarctos cinereus) with bovine serum albumin. This Ig was then purified using the highly specific techniques of affinity chromatography. The purified protein was compared with a "potential" koala Ig protein which was subsequently purified by Protein G chromatography and both proteins were further characterised by agarose/SDS-PAGE and immunoelectrophoresis. Results indicate that koalas do produce Ig in response to antigen challenge, koala Ig has a higher net negative charge than that seen in most other mammals and possibly two subclasses of IgG and IgM are present in normal koala serum.
Wilkinson
R
R
Central Veterinary Laboratories (VETLAB), Department of Agriculture, Adelaide, S.A. Australia.
Allanson
M
M
Kolega
V
V
Lawrence
D
D
Neville
S
S
D000818:Animals; D002846:Chromatography, Affinity; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000302:isolation & purification*; D008297:Male; D008394:Marsupialia / Q000276:immunology*; D011817:Rabbits
D007074:Immunoglobulin G