title: The polymerase chain reaction: basic methodology and applications.
abstract: The "polymerase chain reaction" (PCR) is a high-power molecular biology technique allowing in vitro enzymatic amplification of a given DNA sequence. This exponential amplification can reach 10(7)-10(9), even a single DNA molecule can be detected. Also the use of non-radioactive probes, considered to be less sensitive than their radioactive counterparts, is possible for the molecular hybridization, to retain a high level of sensitivity. PCR is defined as a "free bacteria" cloning technique, which has many applications in fundamental research and in the clinical analysis of genetic disease, infectious diseases and cancers. Thus PCR is a revolutionary method which is capable of greatly stimulating scientific research and modifying the diagnostic area in the near future.
authors.affiliation: EIBET, Plaisir, France.
authors.forename: L Z
authors.initials: LZ
authors.lastname: Xu
authors.forename: D
authors.initials: D
authors.lastname: Larzul
mesh_terms: D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry; D016172:DNA Fingerprinting; D005784:Gene Amplification; D030342:Genetic Diseases, Inborn / Q000175:diagnosis; D007239:Infections / Q000175:diagnosis; D016297:Mutagenesis, Site-Directed; D009369:Neoplasms / Q000175:diagnosis; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests
chemical_list: D004247:DNA