Inhibition of adherence of Giardia intestinalis by human neutrophils and monocytes.
Human neutrophils and monocytes were found to be capable of interfering with the in vitro adherence of Giardia intestinalis. Significantly greater inhibition of adherence was shown by stimulated phagocytic cells than by unstimulated cells. Both azurophil and specific granules extracted from the cytoplasm of neutrophils were equally potent in their adherence inhibitory activity. Dose-dependent effects on adherence were observed with granular enzymes and reactive oxygen species. Lower concentrations of reactive oxygen species were required for adherence inhibition that for growth inhibition. These results suggest that the adherence mechanism of G. intestinalis may be a feasible target for immunological attack by phagocytic cells.
Crouch
A A
AA
Department of Child Health, University of Queensland, Mater Children's Hospital, South Brisbane, Australia.
Seow
W K
WK
Whitman
L M
LM
Smith
S E
SE
Thong
Y H
YH
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D003594:Cytoplasmic Granules / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D005872:Giardia / Q000276:immunology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007957:Leukocyte Adherence Inhibition Test; D009000:Monocytes / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*
D006861:Hydrogen Peroxide