title: Identification of an intermediate compartment involved in protein transport from endoplasmic reticulum to Golgi apparatus.
abstract: We have studied the role of a previously described tubulovesicular compartment near the cis-Golgi apparatus in endoplasmic reticulum (ER)-to-Golgi protein transport by light and immunoelectron microscopy in Vero cells. The compartment is defined by a 53-kDa transmembrane protein designated p53. When transport of the vesicular stomatitis virus strain ts045 G protein was arrested at 39.5 degrees C, the G protein accumulated in the ER but had access to the p53 compartment. At 15 degrees C, the G protein was exported from the ER into the p53 compartment which formed a compact structure composed of vesicular and tubular profiles in close proximity to the Golgi. Upon raising the temperature to 32 degrees C, the G protein migrated through the Golgi apparatus while the p53 compartment resumed its normal structure again. These results establish the p53 compartment as the 15 degrees C intermediate of the ER-to-Golgi protein transport pathway.
authors.affiliation: Department of Pharmacology, University of Basel, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Schweizer
authors.forename: J A
authors.initials: JA
authors.lastname: Fransen
authors.forename: K
authors.initials: K
authors.lastname: Matter
authors.forename: T E
authors.initials: TE
authors.lastname: Kreis
authors.forename: L
authors.initials: L
authors.lastname: Ginsel
authors.forename: H P
authors.initials: HP
authors.lastname: Hauri
mesh_terms: D000818:Animals; D001692:Biological Transport; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D008954:Models, Biological; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure; D013696:Temperature; D014709:Vero Cells; D014721:Vesicular stomatitis Indiana virus / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000378:metabolism*
chemical_list: C028391:G protein, vesicular stomatitis virus; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D014759:Viral Envelope Proteins