[Some chemical and serological properties of perchloric acid-soluble glycoproteins separated from ascitic fluid of a patient with metastatic omentum tumor from ovarian cancer].
One mol perchloric acid-soluble fraction (PASF) obtained from ascitic fluid of a patient (blood group B) with metastatic omentum tumor from ovarian cancer was a glycoprotein fraction containing 35.4% carbohydrate and exhibited A, B and Thomsen-Friedenreich (T) activities. This fraction was separated into three fractions, Frs. 1-3, by a gel filtration with Bio-Gel A-1.5 m column. Among these fractions, Fr. 1 which was obtained in a 5.5% yield to PASF, was a glycoprotein fraction with a high molecular weight, 23.3% carbohydrate and A, B and T activities. Other minor fraction, Fr. 2, and the major fraction, Fr. 3, contained 43.2% and 43.9% carbohydrate, but did not show A, B and T activities, respectively.
Kurihara
M
M
Isehara Research Laboratory, Kanto Chemical Co., Inc., Kanagawa, Japan.
Shimauchi
S
S
Ohyama
K
K
Yamakawa
T
T
Watanabe
H
H
Hirakawa
S
S
Ohkuma
S
S
D000017:ABO Blood-Group System; D000368:Aged; D015295:Antigens, Tumor-Associated, Carbohydrate; D001202:Ascitic Fluid / Q000737:chemistry*; D004187:Disaccharides / Q000032:analysis*; D005260:Female; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D010051:Ovarian Neoplasms / Q000737:chemistry*; D010472:Perchlorates; D012995:Solubility
D000017:ABO Blood-Group System; D015295:Antigens, Tumor-Associated, Carbohydrate; D004187:Disaccharides; D006023:Glycoproteins; D010472:Perchlorates; C016467:Thomsen-Friedenreich antigen