[A simple method of determining 1,25-(OH)2D (calcitriol) in blood].
Simple and effective procedure was developed for solid-phase extraction of 1,25(OH)2D (calcitriol) from blood serum or blood plasma using mini-columns "Silica-Cart C18" which enabled to purify the hormone preparation to the state suitable for its subsequent evaluation by means of RRA and RIA. Separation of 1,25(OH)2D from 25-OHD and purification from lipophilic impurities were carried out on the single mini-column where solvents with gradually decreased polarity were used: water, methanol-water (7:3), hexane-chloroform (9:1), hexane-isopropanol (99:1), hexane-isopropanol (85:15). Concentration of 1,25(OH)2D was adequately estimated in blood serum of patients with chronic kidney insufficiency as well as in blood serum of vitamin D deficient animals.
Sergeev
I N
IN
Terekhina
T A
TA
Iakushina
L M
LM
Spirichev
V B
VB
D000818:Animals; D002117:Calcitriol / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006168:Guinea Pigs; D011863:Radioimmunoassay; D011869:Radioligand Assay; D051381:Rats; D014807:Vitamin D / Q000378:metabolism
D014807:Vitamin D; D002117:Calcitriol
Prostoĭ metod opredeleniia 1,25-)OH)2D (kal'tsitriola) v krovi.