Microtubular response to colchicine in adult and fetal rat hepatocytes.
1. Colchicine and related anti-microtubular drugs impair plasma protein secretion from adult rat liver explants 2-3-fold more than from fetal tissue. 2. Indirect immunofluorescence microscopy of cultured adult and fetal hepatocytes demonstrated that hepatocytes of both ages contain large numbers of densely packed microtubules which are equally disassembled by 10 microM colchicine. 3. Colchicine (10 microM) reduced secretion of [14C]leucine-labelled proteins from cultured adult hepatocytes by about 50% but did not significantly impede fetal secretion. 4. These results confirmed that plasma protein secretion can proceed without an intact microtubular system in fetal hepatocytes.
Kaufman
S S
SS
Swanson Center for Nutrition, Veteran's Administration Medical Center, Omaha, NE.
Blain
P L
PL
Park
J H
JH
Tuma
D J
DJ
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology*; D005260:Female; D005333:Fetus / Q000166:cytology*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008870:Microtubules / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D001798:Blood Proteins; D003078:Colchicine