title: A newly identified iron-binding protein in rat liver: purification and characterization.
abstract: A novel iron-binding protein from rat liver homogenates was purified 1,800-fold with a 5.7% yield, to apparent homogeneity. The molecular weight of the protein was estimated to be 16,000, by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The purified protein exhibited 0.43 mol of iron binding per mol of protein with a dissociation constant (Kd) of 3.5 x 10(-6) M. Al3+ inhibited the iron-binding and the binding was also slightly inhibited by Ni2+. Other divalent metal ions such as Cu2+, Zn2+ and Mn2+ were without effect. Immunoblot analysis of the iron-binding protein revealed that the protein is located mainly in microsomes. This newly identified iron-binding protein may be involved in intracellular transport of iron.
authors.affiliation: Department of Hygiene, Kansai Medical University, Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Furukawa
authors.forename: S
authors.initials: S
authors.lastname: Taketani
authors.forename: H
authors.initials: H
authors.lastname: Kohno
authors.forename: R
authors.initials: R
authors.lastname: Tokunaga
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002458:Cell Fractionation; D004591:Electrophoresis, Polyacrylamide Gel; D007501:Iron / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D007501:Iron