Evaluation of a simplified test for the rapid detection of antibody to the human immunodeficiency virus (HIV-1).
We describe a new simplified solid phase immunoassay for the detection of anti-HIV antibodies. The results obtained analyzing a panel of sera from subjects showing a different pattern of reactivity on enzyme immunoassays and on Western blot demonstrated high sensitivity and specificity. The test does not require experienced laboratory staff nor the use of costly laboratory instruments.
Quinti
I
I
Department of Allergy and Clinical Immunology, University of Rome, La Sapienza, Italy.
Guerra
E
E
Mezzaroma
I
I
Scala
E
E
Rainaldi
L
L
Galluzzo
C M
CM
Aiuti
F
F
D000328:Adult; D000469:Alkaline Phosphatase; D015153:Blotting, Western; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000032:analysis*; D015658:HIV Infections / Q000175:diagnosis* / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000191:economics / Q000295:instrumentation; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic
D015483:HIV Antibodies; D011933:Reagent Kits, Diagnostic; D000469:Alkaline Phosphatase