G proteins. Linkages with amine receptors.
Means of defining specific linkages among receptors and guanosine triphosphate (GTP)-binding regulatory proteins (G proteins) are discussed. The definition of such linkages relies upon the development of suitable G protein-directed antibodies and methods of resolving closely related subtypes of subunits. Conventional means of assessing interactions among receptors and G proteins are described, with an emphasis placed upon their application to receptors for serotonin. Emerging protocols are based upon agonist-effected decreases (or increases) in adenosine diphosphate (ADP) ribosylation, purification of receptor-G protein complexes, disruption of communication among receptors and G proteins, and activation-related alterations in protein conformation. Additional determinants of specificity are cotranslational and posttranslational modifications.
Manning
D R
DR
Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104-6084.
D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D011956:Receptors, Cell Surface / Q000378:metabolism*
D001679:Biogenic Amines; D011956:Receptors, Cell Surface; D019204:GTP-Binding Proteins