Identification of two somatostatin-immunoreactive cell types in the principal islet of Sparus auratus L. (Teleostei) by immunogold staining.
Two types of somatostatin (SST 14)-immunoreactive cells are identified by immunogold staining in the Lowicryl-embedded principal islet of Sparus auratus: D1 cells, having large moderate to low electron dense granules, located between A cells in the islet periphery and D2 cells, containing smaller electron-dense granules, present between B cells in the central region of the islet. Although SST 28-like immunoreactivity was not observed in D cells of S. auratus, the presence of SST 14 and a SST 22-,25-, or 28-like sequence in D2 and D1 cells, respectively, is discussed. A third SST 14-immunoreactive cell, found in the islet periphery, showed immunoreactive D1- and unreactive A-like granules. This cell type, which has a pyknotic-like nucleus and a dark appearance in osmicated Epon-embedded tissue, is supposed to be the product of fusion of D1 and A cells.
Abad
M E
ME
Department of Cell Biology, University of Murcia, Spain.
Lozano
M T
MT
Taverne-Thiele
J J
JJ
Rombout
J H
JH
D000818:Animals; D005399:Fishes / Q000378:metabolism*; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D013004:Somatostatin / Q000096:biosynthesis* / Q000276:immunology
D013004:Somatostatin