Polyalkylenes used as stationary phases for preparative reversed-phase liquid chromatography.
Polyethylene and polypropylene were employed as stationary phases for preparative liquid chromatography and compared with LiChroprep RP-18. Polyalkylene phases are chemically stable and have homogeneous hydrophobic surfaces, but they are physically less resistant than silica-based phases. The influence of organic modifiers and salt ions on the capacity factors of aromatic hydrocarbons and peptides was determined. Peptides prepared by solid-phase peptide synthesis were purified on polypropylene. The results demonstrate the usefulness of polyalkylenes in preparative reversed-phase liquid chromatography, particularly for basic peptides.
Krause
E
E
Institute of Drug Research, Academy of Sciences, Berlin, G.D.R.
Smettan
D
D
Loth
F
F
Herma
H
H
D002853:Chromatography, Liquid / Q000379:methods*; D006838:Hydrocarbons; D010455:Peptides / Q000032:analysis; D011095:Polyethylenes; D011126:Polypropylenes; D012822:Silicon Dioxide
D006838:Hydrocarbons; D010455:Peptides; D011095:Polyethylenes; D011126:Polypropylenes; D012822:Silicon Dioxide