Techniques for experimental rat kidney (and liver) perfusion.
The technique described here allows to perfuse in situ either the left kidney alone, both kidneys or both kidneys and the liver of rats with few operations, without danger of organs damage and with a good control of temperature and pressure. This technique allows a good maintainance of the metabolic activity of the perfused tissues and offers the possibility to collect urine by cannulating the bladder.
Branca
D
D
Department of Biological Chemistry, Padova University.
Scutari
A
A
Giron
F
F
Roberti
M S
MS
Vicenti
E
E
Scutari
G
G
D000818:Animals; D007668:Kidney; D008026:Ligation / Q000379:methods; D008099:Liver; D008928:Mitochondria / Q000378:metabolism; D010477:Perfusion / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains