Single cell assay of exocytosis from adrenal chromaffin cells using "perforated patch recording".
We have combined "perforated patch recording" with phase detection to examine depolarization-induced changes in membrane capacitance (delta Cm) in bovine adrenal chromaffin cells. With this technique, voltage dependent Ca2+ currents and resultant delta Cm's often show little rundown over 1-2 hours even when the free Ca2+ concentration of the pipette is in the millimolar range. By limiting washout of cytosolic components and by maintaining more intact cytosolic Ca2+ buffering, this approach should facilitate the study of stimulus-exocytosis coupling evoked by physiological stimuli which involve cell metabolism and/or membrane receptor triggered second messenger cascades.
Gillis
K D
KD
Department of Medicine, Washington University, St. Louis, MO 63110.
Pun
R Y
RY
Misler
S
S
D000313:Adrenal Medulla / Q000166:cytology / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002462:Cell Membrane / Q000502:physiology; D004594:Electrophysiology; D005089:Exocytosis; D066298:In Vitro Techniques; D008564:Membrane Potentials
D002118:Calcium