Application of modern NMR techniques in the structure determination of microbial transformation products from sterols.
Microbial transformations of cycloartenol, 24-methylenecycloartanol and lanosterol with Mycobacterium sp. (NRRL B-3805) resulted in the isolation and characterization of five C19 steroids. Details of the tremendous structural modifications of these metabolites from their parent compounds were clarified by 2D nmr techniques. The stereochemistry of these novel metabolites was determined by nOe studies.
Lee
S S
SS
School of Pharmacy, College of Medicine, National Taiwan University, Republic of China.
Young
L H
LH
Wang
K C
KC
D007810:Lanosterol / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015394:Molecular Structure; D009161:Mycobacterium / Q000378:metabolism*; D010840:Phytosterols / Q000378:metabolism; D013261:Sterols / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D014315:Triterpenes
D010840:Phytosterols; D013261:Sterols; D014315:Triterpenes; D007810:Lanosterol; C100089:cycloartenol