title: Characterization of adult human astrocytes derived from explant culture.
abstract: Four different human astrocytic cell lines established from either epilepsy surgical specimens or cerebral white matter obtained during thalamotomy for tremor in a patient with multiple sclerosis were characterized using morphologic analysis, ultrastructural attributes, growth characteristics, and immunocytochemical analysis. Immunocytochemical characterization of cultures indicated a mean of 84% of cells contained cytoplasmic glial fibrillary acidic protein (GFAP): to confirm that GFAP(+) cells also proliferated, bromo-deoxyuridine (BrdU) uptake was measured in cell line. Our method of simplified explant culture allows establishment of astrocytic cell lines from a variety of pathologic substrates using limited amounts of human material.
authors.affiliation: Department of Pathology, Cleveland Clinic Foundation, OH 44195.
authors.forename: M L
authors.initials: ML
authors.lastname: Estes
authors.forename: R M
authors.initials: RM
authors.lastname: Ransohoff
authors.forename: J T
authors.initials: JT
authors.lastname: McMahon
authors.forename: B S
authors.initials: BS
authors.lastname: Jacobs
authors.forename: B P
authors.initials: BP
authors.lastname: Barna
mesh_terms: D000328:Adult; D001253:Astrocytes / Q000276:immunology / Q000502:physiology / Q000648:ultrastructure*; D001973:Bromodeoxyuridine / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009103:Multiple Sclerosis / Q000503:physiopathology
chemical_list: D005904:Glial Fibrillary Acidic Protein; D003994:Bucladesine; D001973:Bromodeoxyuridine