Anti-murine IL-6 receptor antibody inhibits IL-6 effects in vivo.
Thrombopoiesis, as well as antibody production, is one of the major events in which interleukin-6 (IL-6) has been reported to be involved. Polyclonal anti-murine IL-6 receptor antibody was prepared to examine the effect of the antibody on these events in IL-6-treated mice. Administration of the anti-mIL-6R antibody inhibited the IL-6-induced increase in the number of platelets. Enhancement of the serum level of DNP-specific antibody by intraperitoneal injection of IL-6 was inhibited completely with simultaneous administration of the anti-mIL-6R antibody. The level of DNP-specific antibody was decreased, even below the basal value, by the higher dose of anti-mIL-6R antibody, indicating its effect also on endogenous IL-6. This work provides evidence that anti-IL-6R antibody inhibits IL-6 function in vivo, and provides an animal model of the therapeutic use of anti-IL-6R antibody for IL-6-related disease.
Suzuki
H
H
Biotechnology Research Laboratory, Tosoh Corporation, Kanagawa, Japan.
Yasukawa
K
K
Saito
T
T
Anzai
M
M
Goitsuka
R
R
Hasegawa
A
A
Ohsugi
Y
Y
Taga
T
T
Kishimoto
T
T
D000818:Animals; D000906:Antibodies / Q000502:physiology; D001792:Blood Platelets / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004140:Dinitrophenols; D004195:Disease Models, Animal; D006168:Guinea Pigs; D006241:Haptens / Q000187:drug effects; D015850:Interleukin-6 / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010976:Platelet Count; D011971:Receptors, Immunologic / Q000276:immunology*; D019947:Receptors, Interleukin-6; D011994:Recombinant Proteins / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000187:drug effects; D014407:Tumor Cells, Cultured; D000093242:Interleukin-6 Inhibitors
D000906:Antibodies; D004140:Dinitrophenols; D006241:Haptens; D015850:Interleukin-6; D011971:Receptors, Immunologic; D019947:Receptors, Interleukin-6; D011994:Recombinant Proteins; D012710:Serum Albumin, Bovine; D000093242:Interleukin-6 Inhibitors; C034638:dinitrophenyl-bovine serum albumin