Quantification of 32P-labeled samples in gel fragments using the flat-bed liquid scintillation counter.
Quantification of 32P in bands after gel electrophoresis was performed using the flat-bed scintillation counter (Betaplate). The most convenient system involved placing fragments of dried gel between two glass fiber sheets, each previously sealed in a thin plastic bag with liquid scintillant. Good pulse-height spectra and counting efficiencies were obtained with low cross talk and background. The method has been used to quantify mRNA in RNA antisense-protection assays that were linear over a wide range (1-20000 cpm). Cross talk and background could be reduced further by an alternative technique utilizing plastic trays with shallow wells in which a solid scintillant had been melted. Fragments were immersed in the molten scintillant (90 degrees C), which was allowed to solidify, by cooling, before counting.
Potter
C G
CG
Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Headington, Oxford, United Kingdom.
Tan
C C
CC
Ratcliffe
P J
PJ
D000818:Animals; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004921:Erythropoietin / Q000235:genetics; D000860:Hypoxia / Q000378:metabolism; D007668:Kidney / Q000737:chemistry; D010761:Phosphorus Radioisotopes / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D012588:Scintillation Counting / Q000295:instrumentation* / Q000379:methods
D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger; D004921:Erythropoietin