[Hybridization of DNA with oligonucleotides immobilized in a gel: a convenient method for recording single base replacements].
In an attempt to develop a reliable system for DNA sequence analysis with multiple hybridization probes, oligonucleotides down to 8 bases long were covalently immobilized in a thin layer of polyacrylamide gel fixed on a glass plate. It was shown possible to detect single base changes in DNA by hybridization of the immobilized oligonucleotides with radioactively and fluorescently labeled DNA fragments. Moreover, it was found that dissociation temperatures of differently GC-rich duplexes could be equalized by appropriate choice of immobilized oligonucleotides concentrations. A model accounting for this phenomenon is presented. In order to make the system more compact, a rectangular matrix of 200 mm dots of immobilized oligonucleotides ("hybridization chip") was designed which offered the sensitivity of 20 attomoles per dot for fluorescent DNA fragment. The applications and perspectives of the approach are discussed.
Khrapko
K R
KR
Khorlin
A A
AA
Ivanov
I B
IB
Chernov
B K
BK
Lysov
Iu P
IuP
Vasilenko
S K
SK
Florent'ev
V L
VL
Mirzabekov
A D
AD
D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000235:genetics*; D013696:Temperature
D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides; D004247:DNA
Gibridizatsiia DNK s oligonukleotidami, immobilizovannymi v gele: udobnyĭ metod registratsii odinochnykh zamen osnovaniĭ.