Modifications of the AOAC total dietary fiber method.
1. The TDF values measured by the modification showed excellent agreement with those determined by the AOAC method. 2. The estimated assay variabilities of the modification were significantly less than those of the AOAC TDF method. 3. The TDF method can be simplified with the simultaneous incubation of a sample with alpha-amylase and amyloglucosidase, followed by subsequent protease incubation without changing pH and temperature. The precision of this shortened method is comparable to that of the AOAC method.
Lee
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Science and Technology Center Kellogg Company, Battle Creek, MI.
Hicks
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D002021:Buffers; D011232:Chemical Precipitation; D004043:Dietary Fiber / Q000032:analysis*; D002523:Edible Grain / Q000032:analysis; D010450:Endopeptidases; D000431:Ethanol; D005504:Food Analysis / Q000379:methods* / Q000592:standards; D005087:Glucan 1,4-alpha-Glucosidase; D014675:Vegetables / Q000032:analysis; D000516:alpha-Amylases
D002021:Buffers; D004043:Dietary Fiber; D000431:Ethanol; D000516:alpha-Amylases; D005087:Glucan 1,4-alpha-Glucosidase; D010450:Endopeptidases