title: [Characteristics of adenosine triphosphatase activity in carp erythrocytes treated with saponin].
abstract: The activities of Ca-ATPase and Na,K-ATPase in saponin-treated erythrocytes of man, rat and carp were compared. It was shown that at free calcium concentrations lower than 1 microM the activity of Ca-ATPase in carp erythrocytes was by one order of magnitude lower than in rat erythrocytes and 3-4 times lower than in human red blood cells. At [Ca2+] = 0.4 microM the activities of Na,K-ATPase in all species under study were essentially the same. The increase in Ca2+ concentration up to 1 microM resulted in a 2-5-fold activation of Na,K-ATPase in rat and carp erythrocytes, respectively. In all cases studied a further elevation of free calcium concentration was accompanied by a decline of the Na,K-ATPase activity. It was shown that the Pi content in carp erythrocytes is 5-6 times as high as that in mammalian cells. This circumstance is a considerable obstacle to a detailed analysis of mechanisms of ATPase activity regulation in carp erythrocytes by methods used for determination of inorganic phosphate production.
authors.forename: G A
authors.initials: GA
authors.lastname: Skriabin
authors.forename: V V
authors.initials: VV
authors.lastname: Petruniaka
authors.forename: S N
authors.initials: SN
authors.lastname: Orlov
authors.forename: S V
authors.initials: SV
authors.lastname: Kotelevtsev
authors.forename: Iu P
authors.initials: IuP
authors.lastname: Kozlov
mesh_terms: D000818:Animals; D002118:Calcium / Q000097:blood; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000097:blood*; D002347:Carps / Q000097:blood*; D004789:Enzyme Activation; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques; D051381:Rats; D012503:Saponins / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000097:blood*; D013045:Species Specificity
chemical_list: D012503:Saponins; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium
vernacular_title: Osobennosti aktivnosti adenozintrifosfataz v éritrotsitakh karpa, obrabotannykh saponinom.