An NMR compatible model for solid tumour.
An NMR model for the study of drug interactions with solid tumour is described. It is based on the use of packed cells in a simple medium. The system is capable of dealing with cellular biochemistry at a molecular level through experiments conducted in a homogeneous field by 1H spin-echo NMR spectroscopy. However, through a simple imaging technique, it is also possible to introduce a spatial element into the chemistry of the packed cells. The use of these complementary NMR methods are illustrated by the action of the cytotoxic agent doxorubicin on cellular glycolysis. A pattern of signals observed in a homogeneous field experiment following oxidative stress are duplicated in the imaging experiment to illustrate the toxins' ability to penetrate the model tumour.
Reglinski
J
J
Department of Pure and Applied Chemistry, Strathclyde University, Glasgow, UK.
al-Kabban
M
M
Smith
W E
WE
Watson
I D
ID
Stewart
M J
MJ
D000970:Antineoplastic Agents / Q000494:pharmacology; D018288:Carcinoma, Small Cell / Q000503:physiopathology; D006801:Humans; D008175:Lung Neoplasms / Q000503:physiopathology; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D008954:Models, Biological; D009369:Neoplasms / Q000188:drug therapy* / Q000503:physiopathology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents