title: The effects of three reproductive hormones and cortisone on the replication of avian infectious bronchitis virus in vitro.
abstract: The in-vitro effects of three reproductive hormones (progesterone, oestrogen testosterone), and cortisone on the replication of infectious bronchitis (IB) virus strain G were investigated over a period of 36 hours using tracheal organ cultures as the culture system. The non-toxic concentration of each hormone for the culture system was first determined. These were found to be 3 micrograms/ml for progesterone, testosterone, and cortisone, and 1 micrograms/ml for oestrogen. The results were based on the assay of the extracellular virus production from both hormone treated and untreated infected cultures at specific intervals up to and including 36 hours. While oestrogen, testosterone, and cortisone were found to enhance the replication of the virus, no significant effect was noticed following treatment with progesterone.
authors.affiliation: Department of Veterinary Medicine, University of Maiduguri, Borno State, Nigeria.
authors.forename: A G
authors.initials: AG
authors.lastname: Ambali
authors.forename: R C
authors.initials: RC
authors.lastname: Jones
mesh_terms: D003348:Cortisone / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology*; D001351:Infectious bronchitis virus / Q000187:drug effects* / Q000254:growth & development; D011374:Progesterone / Q000494:pharmacology*; D013739:Testosterone / Q000494:pharmacology*; D013997:Time Factors; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D004967:Estrogens; D013739:Testosterone; D011374:Progesterone; D003348:Cortisone