title: Serum levels of type III procollagen peptide in Equidae before and after intestinal ischemia.
abstract: Serum levels of type III procollagen peptide (P-III-P) were measured by radioimmunoassay in clinically normal adult ponies (n = 15) and horses (n = 10). The mean serum levels of P-III-P from the ponies, 10.4 +/- 2.9 (SD) ng/mL, and the horses, 12.2 +/- 2.6 (SD) ng/mL, were not significantly different. Segments of jejunum were made ischemic to induce fibrous peritoneal adhesions in two ponies, and serum P-III-P levels were measured on days 4, 5, 7, 14, and 21. An exploratory celiotomy on day 21 revealed that the ischemic injury had induced fibrosis of the mesentery and bowel, but no adhesions had formed. The fibrotic mesentery contained type III collagen. The highest mean serum level of P-III-P, 23.0 +/- 3.5 (SD) ng/mL on day 7, was more than 4 SD above the mean from the normal ponies. There was a significant difference in the serum P-III-P levels in the ponies on days 0 (7.1 +/- 1.6 ng/mL) and 7 (23.0 +/- 3.5 ng/mL). Serum levels of P-III-P may be useful to study fibrosis associated with intestinal ischemia.
authors.affiliation: Department of Clinical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907.
authors.forename: K J
authors.initials: KJ
authors.lastname: Whitehair
authors.forename: J E
authors.initials: JE
authors.lastname: Parker
authors.forename: G N
authors.initials: GN
authors.lastname: Smith
authors.forename: S B
authors.initials: SB
authors.lastname: Adams
authors.forename: G B
authors.initials: GB
authors.lastname: Bottoms
mesh_terms: D000818:Animals; D003720:Densitometry; D006734:Horse Diseases / Q000097:blood* / Q000209:etiology; D006736:Horses; D007511:Ischemia / Q000097:blood / Q000150:complications / Q000662:veterinary*; D007579:Jejunal Diseases / Q000209:etiology / Q000662:veterinary; D007583:Jejunum / Q000098:blood supply* / Q000473:pathology; D010446:Peptide Fragments / Q000097:blood*; D010532:Peritoneal Diseases / Q000209:etiology / Q000662:veterinary; D011347:Procollagen / Q000097:blood*; D000267:Tissue Adhesions / Q000209:etiology / Q000662:veterinary
chemical_list: D010446:Peptide Fragments; D011347:Procollagen; C041876:procollagen Type III-N-terminal peptide