title: In vivo T lymphocyte origin of macrophage-tropic strains of HIV. Role of monocytes during in vitro isolation and in vivo infection.
abstract: Previously published isolation techniques with T cell blasts and monocyte-derived macrophages (MDM) were used to recover HIV from the PBMC of a group of 23 asymptomatic seropositive individuals. Viral isolation was more readily accomplished by MDM coculture resulting in 9 isolates being obtained exclusively by this method (macrophage tropic strains). To determine the in vivo cellular source of these isolates we separated PBMC from 5 of these 9 patients into T lymphocyte and monocyte fractions by flow microfluorometry. These fractions were then analyzed by polymerase chain reaction (PCR) for the presence of HIV-1 proviral DNA. In 4 out of these 5 patients HIV-1 proviral DNA could be detected exclusively in T lymphocytes but not in monocytes, although the virus could be isolated only by MDM coculture. In the remaining patient HIV could be amplified in both T lymphocytes and monocytes. Further phenotypic analysis revealed that, among T lymphocytes, only the CD4+ subset was infected with HIV. We conclude that among PBMC the most common in vivo source of HIV strains which preferentially infect macrophages in vitro is the CD4+ T lymphocyte. These data also suggest that the macrophage tropism characteristic of some HIV strains reflects predominantly an in vitro phenomenon.
authors.affiliation: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
authors.forename: F E
authors.initials: FE
authors.lastname: Massari
authors.forename: G
authors.initials: G
authors.lastname: Poli
authors.forename: S M
authors.initials: SM
authors.lastname: Schnittman
authors.forename: M C
authors.initials: MC
authors.lastname: Psallidopoulos
authors.forename: V
authors.initials: V
authors.lastname: Davey
authors.forename: A S
authors.initials: AS
authors.lastname: Fauci
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000382:microbiology*; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis; D006678:HIV / Q000302:isolation & purification; D015488:HIV Antigens / Q000378:metabolism; D015658:HIV Infections / Q000175:diagnosis; D006679:HIV Seropositivity / Q000382:microbiology*; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D009000:Monocytes / Q000382:microbiology*; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral; D015488:HIV Antigens