The role of selenium in the secretion of very-low-density lipoprotein in the isolated perfused rat liver.
A recirculating liver perfusion system was used to study the effects of dietary selenium (Se) on the hepatic secretion of very-low-density lipoprotein (VLDL). The perfusate from livers of rats fed on a Se-deficient diet incorporated about 50% more [1-14C]oleic acid into triacylglycerol (TG) and cholesteryl esters (ChoEs) than did the perfusate from livers of rats fed on a Se-supplemented diet. Similarly, livers from rats fed the Se-deficient diet secreted more VLDL and incorporated about 60% more [1-14C]oleic acid into VLDL TG and ChoEs than did livers from rats fed the Se-supplemented diet. The liver perfusate from rats in the Se-deficient group also showed significantly decreased fatty acid oxidation. We conclude that Se is a potent modulator of lipoprotein metabolism. A primary action of Se deficiency appears to be a decrease in fatty acid oxidation and a stimulation of fatty acid esterification, leading to increased VLDL TG and ChoEs formation and secretion.
Scott
R L
RL
Department of Biomedical Sciences, Meharry Medical College, Nashville, TN 37208.
Kheshti
A
A
Heimberg
M
M
Wilcox
H G
HG
Stone
W L
WL
D000818:Animals; D001835:Body Weight; D005979:Glutathione Peroxidase / Q000378:metabolism; D066298:In Vitro Techniques; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008099:Liver / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D009929:Organ Size; D010084:Oxidation-Reduction; D010477:Perfusion; D051381:Rats; D011918:Rats, Inbred SHR; D012643:Selenium / Q000172:deficiency / Q000502:physiology*
D008079:Lipoproteins, VLDL; D009829:Oleic Acids; D019301:Oleic Acid; D005979:Glutathione Peroxidase; D012643:Selenium