Isolation and characterization of the rat glutamine synthetase-encoding gene.
From a rat genomic library in phage lambda Charon4A, a complete glutamine synthetase-encoding gene was isolated. The gene is 9.5-10 kb long, consists of seven exons, and codes for two mRNA species of 1375 nucleotides (nt) and 2787 nt, respectively. For both mRNAs, full-length cDNAs containing a short poly(A) tract were identified. The sequences of the entire mRNA and of the exon-intron transitions were determined. The smaller mRNA is identical to the 5' 1375 nt of the long mRNA and contains the entire protein-coding region. The position of the transcription start point was mapped. Within the first 118 bp of promoter sequence, a (T)ATAA-box, a CCAAT-box and an SP1-binding site were identified.
van de Zande
L
L
Department of Anatomy and Embryology, University of Amsterdam, The Netherlands.
Labruyère
W T
WT
Arnberg
A C
AC
Wilson
R H
RH
van den Bogaert
A J
AJ
Das
A T
AT
van Oorschot
D A
DA
Frijters
C
C
Charles
R
R
Moorman
A F
AF
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004247:DNA / Q000235:genetics; D005091:Exons; D005796:Genes; D005974:Glutamate-Ammonia Ligase / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping
D012333:RNA, Messenger; D004247:DNA; D005974:Glutamate-Ammonia Ligase