Transfer of methomyl and HmT-toxin sensitivity from T-cytoplasm maize to tobacco.
The mitochondrial gene, T-urf13, which is unique to the T-cytoplasm of maize, has been expressed in tobacco plants using the Cauliflower Mosaic Virus 35S promoter. Tobacco plants expressing T-urf13 exhibit a variety of responses to methomyl. Leaf discs and petiole sections bleach when exposed to methomyl or HmT-toxin; this effect increases with the age of the tissue. The bleaching effect is not however observed when light is excluded. Plants homozygous for T-urf13 exhibit extreme sensitivity when sprayed with methomyl. The growth of seedling which are either homozygous or heterozygous for T-urf13 is inhibited by methomyl and by kanamycin, whereas seedlings from untransformed tobacco or tobacco which has lost the T-urf13 gene through segregation are sensitive to kanamycin but develop normally when exposed to methomyl. The results demonstrate that T-URF13 need not be specifically targeted to the mitochondrion for it to induce methomyl or HmT-toxin sensitivity in tobacco.
von Allmen
J M
JM
Cambridge Laboratory, John Innes Centre, Norwich, UK.
Rottmann
W H
WH
Gengenbach
B G
BG
Harvey
A J
AJ
Lonsdale
D M
DM
D001483:Base Sequence; D015139:Blotting, Southern; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA; D004351:Drug Resistance / Q000235:genetics; D007613:Kanamycin Resistance / Q000235:genetics; D008027:Light; D008724:Methomyl / Q000494:pharmacology*; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D009183:Mycotoxins / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics*; D014162:Transfection; D003313:Zea mays / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
D024101:Mitochondrial Proteins; D009183:Mycotoxins; D010940:Plant Proteins; C071393:URF13 protein, Zea mays; D008724:Methomyl; D004247:DNA