title: Interaction between cholinergic and opiate receptors of the lateral hypothalamus in the control of renal excretion in rats.
abstract: The present study was carried out to investigate the participation and interaction between cholinergic and opiate receptors of the lateral hypothalamus (LH) in the regulation of Na+, K+ and water excretion. Male Holtzman rats were implanted with chronic cerebral cannulas into the LH. Urine was collected over a period of 2 h after injection of carbachol, FK-p33824 + carbachol or naloxone + carbachol into the LH. Carbachol (8 nmol) reduced urinary volume and increased Na+ excretion. Previous injection of FK-33824 (100 ng) into the LH increased the antidiuretic effect of carbachol, but blocked the increase in Na+ excretion and decreased K+ excretion. Naloxone (10 micrograms) produced no changes in the effect of carbachol on renal excretion. These data show an inhibitory effect of opiate receptors on the changes in urinary Na+ and K+ excretion that are induced by cholinergic stimulation of the LH in rats, and a potentiating effect on antidiuresis.
authors.affiliation: Departamento de Ciências Fisiológicas, Faculdade de Odontologia, Universidade Estadual Paulista, Araraquara, Brasil.
authors.forename: R C
authors.initials: RC
authors.lastname: Queiroz
authors.forename: W A
authors.initials: WA
authors.lastname: Saad
authors.forename: L A
authors.initials: LA
authors.lastname: Camargo
authors.forename: J
authors.initials: J
authors.lastname: Antunes-Rodrigues
authors.forename: A
authors.initials: A
authors.lastname: Renzi
authors.forename: L A
authors.initials: LA
authors.lastname: De-Luca Júnior
authors.forename: J V
authors.initials: JV
authors.lastname: Menani
mesh_terms: D000818:Animals; D002217:Carbachol; D007026:Hypothalamic Area, Lateral / Q000502:physiology*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D009318:Natriuresis / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D011950:Receptors, Cholinergic / Q000502:physiology*; D011957:Receptors, Opioid / Q000502:physiology*; D014882:Water-Electrolyte Balance / Q000187:drug effects*
chemical_list: D011950:Receptors, Cholinergic; D011957:Receptors, Opioid; D002217:Carbachol