Preparation and characterization of basolateral plasma-membrane vesicles from sheep parotid glands. Mechanisms of phosphate and D-glucose transport.
A procedure is described for the preparation of basolateral membrane vesicles from the acinar cells of the sheep parotid gland. The ouabain-sensitive K(+)-activated phosphatase activity was enriched 30-fold over the tissue homogenate; 45% of this activity was recovered in the final membrane fraction. The presence of membranes from other organelles was negligible. Evidence is presented for the location of Na(+)-dependent symporters for phosphate and D-glucose on the basolateral membrane.
Vayro
S
S
Department of Biochemistry, University College of Wales, Aberystwyth, Dyfed, U.K.
Kemp
R
R
Beechey
R B
RB
Shirazi-Beechey
S
S
D000818:Animals; D000838:Anions / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002352:Carrier Proteins / Q000037:antagonists & inhibitors; D002412:Cations / Q000494:pharmacology; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000737:chemistry*; D005260:Female; D005947:Glucose / Q000378:metabolism*; D008297:Male; D010306:Parotid Gland / Q000737:chemistry*; D010710:Phosphates / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012756:Sheep; D012964:Sodium / Q000502:physiology; D013696:Temperature
D000838:Anions; D002352:Carrier Proteins; D002412:Cations; D010710:Phosphates; D012964:Sodium; D005947:Glucose