Thymic nuclear matrix associated activity is not V(D)J recombinase.
It was previously reported that nuclear matrix isolated from young rat thymus contained an activity that supported V(D)J recombination at a high efficiency (Dave et al., BIOCHEMISTRY 30: 4763-4767, 1991). A similar type of activity is also detected in the matrix prepared from fetal calf thymus. However, restriction enzyme mapping analyses of the recombined product clearly suggest that the double antibiotic resistance exhibited by the matrix treated plasmid substrate is not a consequence of V(D)J signal sequence recombination.
Pandey
V N
VN
Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
Dave
V P
VP
Amrute
S B
SB
Rosenbach
K
K
Modak
M J
MJ
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000201:enzymology*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004254:DNA Nucleotidyltransferases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004352:Drug Resistance, Microbial / Q000235:genetics; D005333:Fetus; D005821:Genetic Techniques; D015530:Nuclear Matrix / Q000201:enzymology; D010957:Plasmids; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D013950:Thymus Gland / Q000201:enzymology*; D045567:VDJ Recombinases
D011994:Recombinant Proteins; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase; D004254:DNA Nucleotidyltransferases; D045567:VDJ Recombinases