Effects of antioxidants on V79 Chinese hamster cells treated with ferric nitrilotriacetate.
The cytotoxic effects of ferric nitrilotriacetate (Fe-NTA) have been considered to be caused by free radicals produced by the drug. The present study was carried out to determine whether or not cytotoxic effects of Fe-NTA on cell growth and lipoperoxide formation of Chinese hamster cells were reduced by antioxidants. Using a spin trapping technique, we found that hydroxyl radical formation in the cells increased in the presence of Fe-NTA. Antioxidants, with the exception of superoxide dismutase, slightly inhibited production of the hydroxyl radical. Mannitol significantly reduced lipoperoxide formation, but other antioxidants did not. However, the growth inhibitory effects of Fe-NTA were not attenuated by these antioxidants. These results indicated that the cytotoxic effects of Fe-NTA may be mostly due to unknown factors other than oxygen free radicals.
Takehara
Y
Y
Medical Science Laboratory, Kurashiki Center for Adult Diseases, Japan.
Yoshioka
T
T
Namba
M
M
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D005290:Ferric Compounds / Q000633:toxicity*; D006878:Hydroxides; D017665:Hydroxyl Radical; D015227:Lipid Peroxidation / Q000187:drug effects; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000633:toxicity; D013482:Superoxide Dismutase / Q000494:pharmacology
D000975:Antioxidants; D005290:Ferric Compounds; D006878:Hydroxides; D017665:Hydroxyl Radical; D013482:Superoxide Dismutase; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate