Correlation of electrophoretic mobilities from capillary electrophoresis with physicochemical properties of proteins and peptides.
Excellent correlation was observed for the electrophoretic mobilities measured by capillary zone electrophoresis versus q/MW2/3, where q is the calculated charge and MW is the molecular weight. Mobilities of a set of 33 diverse peptides from enzymatic digests and 10 intact proteins were measured for separations at pH 2.35, 8.0, and 8.15 with constant ionic strength, temperature, and viscosity. The correlation suggests that the frictional drag is proportional to the surface area of a sphere that has a volume proportional to the MW. The correlation of electrophoretic mobility with physicochemical properties will facilitate the elucidation of optimum separation strategies for protein and peptide mixtures.
Rickard
E C
EC
Eli Lilly and Company, Lilly Research Laboratories, Indianapolis, Indiana 46285.
Strohl
M M
MM
Nielsen
R G
RG
D000596:Amino Acids / Q000737:chemistry; D002021:Buffers; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004563:Electrochemistry; D004586:Electrophoresis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*
D000596:Amino Acids; D002021:Buffers; D010455:Peptides; D011506:Proteins