Isolation and characterization of intragenic suppressors of an Escherichia coli ftsA mutation.
Mutagenesis of a strain of Escherichia coli carrying a temperature-sensitive (Ts) mutation in the cell division gene ftsA yielded a number of temperature-resistant variants. In certain cases, restoration of viability at the restrictive temperature could not be attributed to suppressor mutations occurring in other genes or to structural gene reversion. DNA sequencing of the variants demonstrated the continuing presence of the original Ts mutation (ftsA13) and revealed secondary mutations within the same gene. These secondary mutations are able to rescue the ftsA13 mutation in cis, but not in trans.
Robinson
A C
AC
Department of Medicine, Royal Infirmary, Edinburgh, Scotland, UK.
Begg
K J
KJ
MacArthur
E
E
D001345:Autoradiography; D001483:Base Sequence / Q000235:genetics; D002455:Cell Division / Q000502:physiology; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics*; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000235:genetics*; D013489:Suppression, Genetic / Q000235:genetics*; D013696:Temperature; D014161:Transduction, Genetic / Q000502:physiology
D004269:DNA, Bacterial