A chemically-defined medium for organotypic slice cultures.
Organotypic slice cultures provide an excellent system for the analysis of study of the molecular mechanisms of this development necessitates the use of a chemically defined culture medium. We report here the development of a medium, EOL1 defined medium, designed specifically for this purpose. Cultures of both cerebral cortex and basal forebrain demonstrate that this defined medium allows a high degree of cytoarchitectural maintenance while promoting neural metabolism and process outgrowth.
Annis
C M
CM
Department of Anatomy and Neurobiology, College of Medicine, University of California, Irvine 92717.
Edmond
J
J
Robertson
R T
RT
D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology* / Q000378:metabolism; D003470:Culture Media; D007150:Immunohistochemistry; D018377:Neurotransmitter Agents / Q000378:metabolism; D009924:Organ Culture Techniques / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains
D003470:Culture Media; D018377:Neurotransmitter Agents