title: Hybrid genes in the analysis of transformation conditions. 3. Temporal/spatial fate of NPTII gene integration, its inheritance and factors affecting these processes in Nicotiana plumbaginifolia.
abstract: Freshly isolated haploid mesophyll protoplasts of Nicotiana plumbaginifolia were transformed for kanamycin resistance. In 38% of the 224 transformants analysed, transmission of the NPTII gene occurred as a homozygous trait, while 62% of the transformants were heterozygous for the trait. In the first case, the foreign DNA integration predominantly (95%) resulted in monogenic inheritance. The second group was characterized by a significant (46%) proportion of multiple insertions. However, there was no clear-cut difference in the integration pattern between the two groups. Furthermore, transformation rates were increased by 4- to 10-fold when transformed diploid protoplasts were treated with UV light or with 3-aminobenzamide. The number of insertion sites was also increased by these treatments. These results shed further light on the fate of the foreign DNA in transformed plants and on means to control or manipulate the integration event(s).
authors.affiliation: Laboratorium voor Plantengenetica, Vrije Universiteit Brussel, IMOL, Sint-Genesius-Rode, Belgium.
authors.forename: G B
authors.initials: GB
authors.lastname: Gharti-Chhetri
authors.forename: W
authors.initials: W
authors.lastname: Cherdshewasart
authors.forename: J
authors.initials: J
authors.lastname: Dewulf
authors.forename: J
authors.initials: J
authors.lastname: Paszkowski
authors.forename: M
authors.initials: M
authors.lastname: Jacobs
authors.forename: I
authors.initials: I
authors.lastname: Negrutiu
mesh_terms: D001549:Benzamides / Q000494:pharmacology; D004261:DNA Replication; D004251:DNA Transposable Elements; D019868:Kanamycin Kinase; D007613:Kanamycin Resistance / Q000235:genetics; D010770:Phosphotransferases / Q000235:genetics*; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D010947:Plants, Toxic; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics; D014170:Transformation, Genetic / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D001549:Benzamides; D004251:DNA Transposable Elements; C025160:3-aminobenzamide; D010770:Phosphotransferases; D019868:Kanamycin Kinase