Preparative liquid chromatographic separation of the oligomers of nonoxynol-9 and their characterization by 1H-NMR and mass spectroscopy.
Conditions are described for the preparative LC separation of a bulk nonoxynol-9 material into 16 components. 1H-NMR analyses of these fractions provided evidence for all but the first of the components to be oligomers of nonylphenoxypolyethoxyethanol (nonoxynol) with n-values for (OCH2CH2)n ranging consecutively from 3 to 17, corresponding to the LC fractions 2-16. Mass spectral analysis of the separated LC fractions confirmed the oligomeric sizes deduced from 1H-NMR spectral data, and provided EI fragmentation information for these oligomeric substances.
Black
D B
DB
Bureau of Drug Research, Health and Welfare Canada, Ottawa, ON.
Dawson
B A
BA
Ethier
J C
JC
Neville
G A
GA
D002853:Chromatography, Liquid; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008970:Molecular Weight; D017137:Nonoxynol; D011092:Polyethylene Glycols / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D017137:Nonoxynol; D011092:Polyethylene Glycols