Effect of platelet activating factor on prostaglandin release from ovine endometrial cells in culture.
Platelet activating factor (PAF) added in vitro to ovine endometrial cells in primary culture caused a dose-dependent increase in the release of prostaglandin (PG) E into the medium compared with release from untreated cells. At a concentration of 1000 ng/ml of PAF, PGE levels in treatment dishes were significantly higher (P less than 0.05) than those in control dishes [130 +/- 8% vs 100% (mean +/- SEM, N = 5 ewes)]. PAF did not alter the release of PGF2 alpha by the same cells. By contrast, the ovine trophoblast interferon, ovine trophoblast protein-1 (oTP-1, 1 ng/ml) attenuated the release of both PGE and PGF2 alpha and this was not overcome by the presence of PAF (100 ng/ml). Thus does not appear that PAF contributes to the antiluteolytic signal in sheep by a direct action on release of PGF2 alpha although it could influence implantation via stimulation of PGE.
Salamonsen
L A
LA
Prince Henry's Institute of Medical Research, South Melbourne, Australia.
D000818:Animals; D002478:Cells, Cultured; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D010972:Platelet Activating Factor / Q000494:pharmacology*; D011458:Prostaglandins E / Q000378:metabolism*; D012756:Sheep
D010972:Platelet Activating Factor; D011458:Prostaglandins E