Solubilization, identification, and localization of vitellogenin-binding sites in follicles of the cockroach, Nauphoeta cinerea.
Binding sites for vitellogenin were solubilized and analyzed either with a filter assay or with ligand blotting. We tested sodium dodecylsulfate (SDS), Chaps, octyl-beta-D-glucoside, and sodium deoxycholate and found SDS and sodium deoxycholate to be most effective in solubilizing both high and low molecular weight binding sites. In the filter assay the sodium deoxycholate extracts, but not the SDS extracts, maintained binding activity after dilution of the solubilizer below its critical micellar concentration. In ligand blotting we consistently observed, in vitellogenic follicles, binding sites with an apparent Mr of approximately 200,000, 35,000, and three closely spaced bands between 14,000 and 20,000. Binding of vitellogenin to all binding sites was suppressed in the presence of the drug suramin. Analysis of corpora lutea and oothecae as well as of ovariole sheath, follicle cell/basal lamina, and oocyte plasma membrane preparations showed that the 35 and 14-20 kDa binding sites are located in the outer follicle compartments, and the 200 kDa binding site in the oocyte plasma membrane. In the latter we occasionally also observed binding sites with an apparent Mr of approximately 150,000, 95,000, 67,000 and 30,000, particularly at stages after ovulation. The 35 and 14-20 kDa binding sites, as visualized in stained gels and in ligand blotting, are rather abundant and were also seen in several other male and female tissues of Nauphoeta and even in other species. They also bound other 14C-labeled hemolymph proteins and thus appear to be rather unspecific. As binding analysis with nonsolubilized and sodium deoxycholate-solubilized membranes revealed that the quantity of vitellogenin bound by binding sites of the outer follicle compartments was low, it is conceivable that the abundance of the 14-20 kDa and 35 kDa binding sites in ligand blotting is merely an effect of SDS and does not reflect the in vivo situation. We suppose that the 200 kDa binding site of the oocyte plasma membrane represents the vitellogenin receptor involved in endocytosis.
Indrasith
L S
LS
Division of Animal Physiology, University of Berne, Switzerland.
Kindle
H
H
Lanzrein
B
B
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000378:metabolism; D003058:Cockroaches / Q000378:metabolism*; D004527:Egg Proteins; D005260:Female; D007700:Kinetics; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D009036:Moths / Q000187:drug effects / Q000254:growth & development*; D010053:Ovary / Q000378:metabolism; D010060:Ovulation; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D014819:Vitellogenins / Q000378:metabolism*
D002250:Carbon Radioisotopes; D004527:Egg Proteins; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D014819:Vitellogenins; C030474:vitellogenin receptor