Synergistic action of tiazofurin and retinoic acid on differentiation and colony formation of HL-60 leukemia cells.
Tiazofurin and retinoic acid synergistically induced differentiation and inhibited colony formation in HL-60 human promyelocytic leukemia cells in cell culture. The synergism was the result of different mechanisms of action, since the effect of tiazofurin, unlike that of retinoic acid, was prevented by addition of guanosine. Since it has been shown that tiazofurin down-regulated the expression of c-Ki-ras oncogene, and retinoic acid that of the myc oncogene, the joint impact of these drugs is of clinical interest particularly in end-stage leukemia where the therapeutic usefulness of tiazofurin has recently been demonstrated.
Yamaji
Y
Y
Laboratory for Experimental Oncology, Indiana University School of Medicine, Indianapolis 46202-5200.
Natsumeda
Y
Y
Yamada
Y
Y
Irino
S
S
Weber
G
G
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002999:Clone Cells / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006151:Guanosine / Q000494:pharmacology; D006801:Humans; D007168:IMP Dehydrogenase / Q000378:metabolism; D015473:Leukemia, Promyelocytic, Acute / Q000201:enzymology / Q000473:pathology*; D015394:Molecular Structure; D010641:Phenotype; D012254:Ribavirin / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D012263:Ribonucleosides / Q000494:pharmacology*; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D012263:Ribonucleosides; D006151:Guanosine; D012254:Ribavirin; D014212:Tretinoin; D007168:IMP Dehydrogenase; C033706:tiazofurin