Isolation of anonymous, polymorphic DNA fragments from human chromosome 22q12-qter.
A series of 195 random chromosome 22-specific probes, equivalent to approximately 1% of the size of this chromosome, have been isolated from a chromosome 22-specific bacteriophage lambda genomic library. These probes were mapped to four different regions of chromosome 22 on a panel of five somatic cell hybrids. Restriction fragment length polymorphisms were detected by 28 of the probes mapping to 22q12-qter. Evolutionarily conserved sequences in human, mouse, and Chinese hamster DNA were detected by 12% of the isolated probes.
Dumanski
J P
JP
Ludwig Institute for Cancer Research, Stockholm, Sweden.
Geurts van Kessel
A H
AH
Ruttledge
M
M
Wladis
A
A
Sugawa
N
N
Collins
V P
VP
Nordenskjöld
M
M
D015139:Blotting, Southern; D002892:Chromosomes, Human, Pair 22; D015342:DNA Probes / Q000302:isolation & purification*; D006801:Humans; D006822:Hybrid Cells; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D015342:DNA Probes