title: [Effects of somatostatin on human thyroid folliculi in vitro].
abstract: Somatostatin (SRIF) inhibits calcitonin and T3-T4 secretion in thyroid. We have investigated the in vitro effect of SRIF on the basal and TSH induced [3H]thy incorporation, thyroglobulin (tgb) RNA and cAMP level in follicular cells, isolated from normal and adenomatous human thyroids. [3H]thy uptake has been evaluated as TCA-precipitable material in 2, 4, 8, 24 h incubated follicles and 24 h incubated adherent cells. Tgb RNA has been quantified with cytoplasmic dot blot hybridization and cAMP level with RIA method. SRIF reduces basal and TSH-induced [3H]thy in both suspension follicles and epithelial adherent cells. However it does not modify tgb RNA nor cAMP levels in incubated follicles. These data suggest a direct antiproliferative effect of SRIF on human thyroid.
authors.affiliation: Centro di Studi Biochimici delle Patologie del Genoma Umano, Università.
authors.forename: S
authors.initials: S
authors.lastname: Hanau
authors.forename: R
authors.initials: R
authors.lastname: Rossi
authors.forename: R
authors.initials: R
authors.lastname: Piva
authors.forename: I
authors.initials: I
authors.lastname: Maestri
authors.forename: A
authors.initials: A
authors.lastname: Margutti
authors.forename: G
authors.initials: G
authors.lastname: Trasforini
authors.forename: G
authors.initials: G
authors.lastname: Pansini
authors.forename: E
authors.initials: E
authors.lastname: Degli Uberti
authors.forename: L
authors.initials: L
authors.lastname: del Senno
mesh_terms: D006801:Humans; D066298:In Vitro Techniques; D013004:Somatostatin / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism*
chemical_list: D013004:Somatostatin; D013936:Thymidine
vernacular_title: Effetti della somatostatina su follicoli tiroidei umani in vitro.