[Rapid diagnosis of enterovirus 70 infection by detection of IgM antibodies with a biotin-avidin ELISA].
A biotin-avidin immunoassay was developed to detect IgM antibodies to enterovirus 70 (EV70) in sera from patients with acute hemorrhagic conjunctivitis. Biotinylated monoclonal antibody to EV70 and horseradish peroxidase-streptavidin were used in an indirect IgM capture ELISA. 40 acute- and convalescent-phase serum pairs were tested and 34 sera (85.0%) in the convalescent phase were positive for IgM antibodies. The positive rate of the method was much higher than that of the non-biotin-avidin system for EV70 (55%). Two sera in the acute phase, which were negative for neutralizing antibodies, had IgM antibodies. Two serum specimens which had been collected a third time from 2 patients during the seventh week after onset were positive for IgM, showing that EV70 IgM lasts at least for one month and a half. The biotin-avidin ELISA is sensitive and can be performed in 2 days instead of 3 days for the non-biotin-avidin method, and is useful for rapid diagnosis of EV70 infection.
Sawada
H
H
Hokkaido Institute of Public Health, Sapporo, Japan.
Aoki
K
K
D000914:Antibodies, Viral / Q000032:analysis*; D001360:Avidin; D001710:Biotin; D003232:Conjunctivitis, Acute Hemorrhagic / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D004770:Enterovirus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D011237:Predictive Value of Tests
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D001360:Avidin; D001710:Biotin