title: Distribution of insertion sequence IS200 in Salmonella and Shigella.
abstract: Two DNA probes for the detection of insertion sequence IS200 by either Southern blotting or colony hybridization were constructed. One of the probes is a 300 bp EcoRI-HindIII fragment of IS200 cloned onto pBluescript KS(+); the other is a tail-to-tail dimer of the same fragment cloned onto pUC19. A survey of the presence of IS200 among enteric bacteria revealed that more than 90% of the pathogenic or food-poisoning isolates of Salmonella spp. examined contained one or more copies of insertion sequence IS200, with the exception of the subgenus I serovar S. agona in which IS200 is not found. Although insertion sequence IS200 was first considered a Salmonella-specific element, it also exists in many isolates of Shigella sonnei and Shigella flexneri, but not in Shigella dysenteriae.
authors.affiliation: Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Gibert
authors.forename: J
authors.initials: J
authors.lastname: Barbé
authors.forename: J
authors.initials: J
authors.lastname: Casadesús
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000235:genetics* / Q000302:isolation & purification; D012760:Shigella / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial