[Covalent fixation in vivo of trenbolone acetate and estradiol to hepatic DNA of the rat: comparative study of the fixation of tritiated molecules and post labeling with radiophosphorus].
In vivo covalent binding of trenbolone and estradiol was assayed using either radiolabeled compounds or 32P-post labeling. The covalent binding index, as measured with tritiated molecules, was 2.4 for the alpha isomer of trenbolone, 5.4 for its beta isomer and 5.4 for 17-beta estradiol. Using 32P-post labeling at repeated medium doses or a single high dose did not allow any of the 3 compounds to reveal specific adducts in the background of adducts spontaneously formed in control animals. It can therefore be concluded that these steroids most probably do not have a direct genotoxic action.
Petit
C
C
Ecole nationale vétérinaire, Laboratoire de toxicologie biochimique et métabolique, Toulouse, France.
Perin
F
F
Tardieu
D
D
Burgat
V
V
Rico
A G
AG
D045930:Anabolic Agents / Q000378:metabolism*; D000818:Animals; D004247:DNA / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D008099:Liver / Q000737:chemistry*; D008297:Male; D010761:Phosphorus Radioisotopes; D051381:Rats; D011919:Rats, Inbred Strains; D014204:Trenbolone Acetate / Q000031:analogs & derivatives* / Q000378:metabolism
D045930:Anabolic Agents; D010761:Phosphorus Radioisotopes; D004958:Estradiol; D004247:DNA; D014204:Trenbolone Acetate
Fixation covalente in vivo de la trenbolone et de l'estradiol à l'ADN hépatique de rat: étude comparative de la fixation de molécules tritiées et du postmarquage au radiophosphore.