title: Purification and partial amino acid sequence of human aconitase.
abstract: Aconitase has been purified from membranes prepared from both the human gastric carcinoma cell line Okajima and from porcine gastric mucosa by chromatography on concanavalin A-Sepharose and carboxymethyl-Sepharose, and preparative polyacrylamide gel electrophoresis. Automated Edman degradation of the intact proteins yielded no N-terminal amino acid sequence due, presumably, to N-terminal blockage. Sequence analysis of tryptic peptides derived from S-carboxymethyl porcine and human aconitases established the positions of 95 and 64 amino acid residues, respectively. The amino acid sequence data for porcine aconitase was in perfect agreement with the previously reported cDNA-deduced amino acid sequence [Zheng et al. (1990) J Biol Chem 265:2814-2821]. Comparison of the human amino acid sequence data with the cDNA-deduced amino acid sequence of porcine aconitase indicated that these two proteins have 95% amino acid sequence identity within the sequenced region.
authors.affiliation: Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Victoria, Australia.
authors.forename: G S
authors.initials: GS
authors.lastname: Baldwin
authors.forename: K L
authors.initials: KL
authors.lastname: Seet
authors.forename: J
authors.initials: J
authors.lastname: Callaghan
authors.forename: G
authors.initials: G
authors.lastname: Toncich
authors.forename: B H
authors.initials: BH
authors.lastname: Toh
authors.forename: R L
authors.initials: RL
authors.lastname: Moritz
authors.forename: M R
authors.initials: MR
authors.lastname: Rubira
authors.forename: R
authors.initials: R
authors.lastname: Simpson
mesh_terms: D000154:Aconitate Hydratase / Q000737:chemistry* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
chemical_list: D000154:Aconitate Hydratase