Mutational analysis of the HTLV-I trans-activator, Tax.
The gene coding for the trans-activating factor (Tax) of the human T-cell leukemia virus, type I (HTLV-I) was mutagenized in vitro using oligonucleotide-directed mutagenesis and recombinant DNA techniques. All except one of the mutagenized tax constructs failed to trans-activate the HTLV-I LTR in a eukaryotic test system. Moreover, negative Tax mutant Arg-39----Gly was found to be trans-dominant. This observation suggests that Tax contains distinct functional domains mediating different interactions of the protein in the process of trans-activation.
Heder
A
A
Institute of Biochemistry, Biological Research Center, Szeged, Hungary.
Paca-Uccaralertkun
S
S
Boros
I
I
D001483:Base Sequence; D015153:Blotting, Western; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D016356:Gene Products, tax / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D014162:Transfection
D016356:Gene Products, tax; D015500:Chloramphenicol O-Acetyltransferase