title: Adoptive transfer of diabetes in BB rats induced by CD4 T lymphocytes.
abstract: Unseparated splenocytes (SPCs) or purified SPC subsets from diabetes-prone BB (BBdp) or diabetic BB (BBd) rats were activated in vitro with either phorbol myristate acetate (PMA) and ionomycin (I) or concanavalin A (ConA). Such activated SPCs were then injected intravenously into 30-day-old BBdp rats, and their capacity to induce adoptive transfer (AT) of diabetes was studied. The proliferative response in vitro of BBd unseparated SPCs or purified W3/13+ SPCs (i.e., T lymphocytes + large granular lymphocytes) to PMA + I far exceeded that of ConA, resulting in mean stimulation indices of 68 and 112 (PMA + I) and 1.9 and 30 (ConA). The incidence of AT was similar when equal numbers of unseparated SPCs from the same BBd donor were injected after activation by either PMA + I + interleukin 2 (PII) or ConA (57 vs. 50%, respectively); however, injection of PII-activated and macrophage-depleted W3/13+ SPCs from BBd animals resulted in a significantly higher incidence of AT (90%, P less than 0.05). As few as 0.5 x 10(6) PII-activated W3/13+ SPCs were sufficient to induce AT. Sixteen percent of recipients developed diabetes after injection of activated W3/13+ cells from 40-day-old BBdp donors. To determine which W3/13+ cells might mediate such transfer, purified and PII-preactivated CD4 T lymphocytes from BBd rats were injected, and they succeeded in AT in 44% of the recipients. Preactivated BBd B lymphocytes were unable to induce AT. Although a possible role for large granular lymphocytes cannot be excluded, the results demonstrate that in the BB rat, the beta-cell destruction can be induced by CD4 T lymphocytes.
authors.affiliation: McGill Nutrition and Food Science Centre, Royal Victoria Hospital, Montreal, Quebec, Canada.
authors.forename: M D
authors.initials: MD
authors.lastname: Métroz-Dayer
authors.forename: A
authors.initials: A
authors.lastname: Mouland
authors.forename: C
authors.initials: C
authors.lastname: Brideau
authors.forename: D
authors.initials: D
authors.lastname: Duhamel
authors.forename: P
authors.initials: P
authors.lastname: Poussier
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000648:ultrastructure; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002469:Cell Separation; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000276:immunology* / Q000503:physiopathology; D005260:Female; D007116:Immunization, Passive; D007267:Injections; D015759:Ionomycin / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D008297:Male; D051381:Rats; D011913:Rats, Inbred BB / Q000276:immunology*; D011919:Rats, Inbred Strains / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000648:ultrastructure; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000276:immunology / Q000648:ultrastructure; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D003208:Concanavalin A; D015759:Ionomycin; D004247:DNA; D013755:Tetradecanoylphorbol Acetate