Corneal cell adhesion and proliferation on hydrogel sheets bound with cell-adhesive proteins.
The efficiency of protein immobilization to provide a hydrogel surface with a high affinity for corneal epithelial cells was investigated. The cell adhesive proteins collagen and fibronectin, and the cell adhesive oligopeptide RGDS (arginine-glycine-aspartic acid-serine) were successfully covalently immobilized on poly(vinyl alcohol) (PVA) hydrogel sheets. Corneal epithelial cells and L-929 cells were applied to these surfaces. A large number of cells did adhere and proliferate on these protein-bound surfaces in comparison with the virgin PVA not so immobilized.
Kobayashi
H
H
Research Center for Biomedical Engineering, Kyoto University, Japan.
Ikada
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000502:physiology*; D003315:Cornea / Q000166:cytology / Q000502:physiology*; D004847:Epithelial Cells; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D005353:Fibronectins / Q000502:physiology*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D051379:Mice; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000502:physiology*; D011092:Polyethylene Glycols / Q000737:chemistry; D011817:Rabbits
D005353:Fibronectins; D009842:Oligopeptides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; D003094:Collagen; C046617:arginyl-glycyl-aspartyl-serine