title: Binding of cholecystokinin and somatostatin to isolated porcine gastric mucosal cells and effects on aminopyrine uptake.
abstract: Mucosal cells were prepared by enzymatic digestion of porcine gastric mucosa with pronase and collagenase. The resulting cell suspension contained 10-15% parietal cells, which responded to histamine stimulation by an up to 20-fold increase in [14C]aminopyrine accumulation over control levels. Cholecystokinin-8 (CCK-8) evoked a more moderate stimulation of [14C]aminopyrine accumulation, whereas somatostatin inhibited histamine-stimulated accumulation. Parietal cells were enriched by elutriation and isopycnic centrifugation on density gradients of Percoll. A fraction with 60% parietal cells bound approximately three times more iodinated CCK-8 than a fraction containing 70% non-parietal cells. Binding of [125I]BH-CCK-8 to preparations containing 30-60% parietal cells was specifically inhibited to about 50% by 10(-9) M unlabelled CCK-8 but not by bombesin. Cell fractions containing about 30% parietal cells also bound [125I]somatostatin. Unlabelled somatostatin at 10(-9) M inhibited tracer binding by about 50%, while CCK-8 did not affect somatostatin binding to such a preparation. The results suggest the existence of specific receptors for CCK and somatostatin on porcine parietal cells exerting a regulatory influence on acid secretion.
authors.affiliation: Department of Drugs, National Board of Health and Welfare, Uppsala, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Sjödin
authors.forename: L J
authors.initials: LJ
authors.lastname: Englund
authors.forename: S
authors.initials: S
authors.lastname: Mårdh
mesh_terms: D000632:Aminopyrine / Q000493:pharmacokinetics*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002766:Cholecystokinin / Q000378:metabolism* / Q000494:pharmacology; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D011949:Receptors, Cholecystokinin / Q000378:metabolism; D017981:Receptors, Neurotransmitter / Q000378:metabolism; D017481:Receptors, Somatostatin; D013004:Somatostatin / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D011949:Receptors, Cholecystokinin; D017981:Receptors, Neurotransmitter; D017481:Receptors, Somatostatin; D000632:Aminopyrine; D013004:Somatostatin; D006632:Histamine; D002766:Cholecystokinin