Polyadenylated messenger RNA in paired helical filament-immunoreactive neurons in Alzheimer disease.
An antibody raised against isolated paired helical filaments (PHF) was used to identify tangle-bearing (PHF+) neurons in autopsy brain tissue from six Alzheimer disease (AD) patients and six age-matched controls (AMC). A comparison of the levels of polyadenylated messenger RNA [poly(A)+ mRNA] in PHF+ and PHF- neurons of similar cross-sectional area in temporal and parietal lobe and cerebellum from four AD and four AMC brains was made by analysis of in situ hybridization of [3H] polyuridylate [poly(U)] to intracellular poly(A)+ mRNA. In PHF+ neurons, the level of poly(A)+ mRNA was approximately two-thirds that in similar-sized PHF- neurons in either AD or AMC. The level of poly(A)+ mRNA in PHF- neurons in regions of the brain that have more of the histopathologically defined effect in AD was similar to that in regions with less effects.
Griffin
W S
WS
Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock 72205.
Ling
C
C
White
C L
CL
Morrison-Bogorad
M
M
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009454:Neurofibrils / Q000032:analysis / Q000473:pathology*; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000032:analysis*; D011072:Poly U; D012333:RNA, Messenger / Q000032:analysis*
D012333:RNA, Messenger; D011061:Poly A; D011072:Poly U