Influence of docosahexaenoic acid on phosphatidylinositol metabolism in cultured cardiomyocytes.
Polyunsaturated fatty acids are involved at several steps in the turnover of phosphatidylinositol mediated by alpha 1-adrenoceptors. We have cultured neonatal rat cardiomyocytes in a medium containing docosahexaenoic acid (C22:6n3) and have investigated the effects produced by the change in the fatty acid composition of phosphatidylinositol on alpha 1-adrenoceptor-mediated phosphatidylinositol metabolism. The experimental cells, in which phosphatidylinositol was significantly enriched in docosahexaenoic acid and depleted in arachidonic acid, showed a reduced ability to incorporate labeled inositol into phospholipids in comparison with control cells, both in basal conditions and after stimulation by phenylephrine. This decreased incorporation led to a reduced availability of inositol phospholipids, substrates of phospholipase C, and to a reduced production of inositol phosphates under basal conditions.
Bordoni
A
A
Dipartimento di Biochimica, Universita' di Bologna, Italy.
Tantini
B
B
Clo
C
C
Turchetto
E
E
D000818:Animals; D002478:Cells, Cultured; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D005994:Glycerophosphates / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010656:Phenylephrine / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism*; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects / Q000378:metabolism
D005994:Glycerophosphates; D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D011942:Receptors, Adrenergic, alpha; D010656:Phenylephrine; D004281:Docosahexaenoic Acids