Identification of two distinct subfamilies of alpha satellite DNA that are highly specific for human chromosome 15.
We report the isolation of two distinct subfamilies of alpha satellite DNA (pTRA-20 and -25) from human chromosome 15. In situ hybridization experiments indicated that both subfamilies are highly specific for this chromosome. Southern analysis of a somatic hybrid cell line carrying human chromosome 15 revealed a likely higher-order genomic band of 2.5 kb for pTRA-20. Similar analysis for pTRA-25 showed multiple higher-order bands of 3.5, 4.5, and 5 kb at moderately high hybridization stringency, but a predominance of the 4.5-kb species at very high stringency. Direct comparison with human genomic DNA confirmed the authenticity of these higher-order structures and demonstrated polymorphic variations using both probes. The origin of the different alphoid subfamilies on chromosome 15 is discussed. These sequences should be useful for the construction of centromere-based genetic linkage maps for human chromosome 15 and, in conjunction with the other alphoid sequences already reported for chromosomes 13, 14, 21, and 22, should allow a concerted analysis of the evolution and the possible etiological role of these DNAs in aberrations commonly seen in these chromosomes.
Choo
K H
KH
Murdoch Institute for Research in Birth Defects, Royal Children's Hospital, Parkville, Australia.
Earle
E
E
Vissel
B
B
Filby
R G
RG
D015139:Blotting, Southern; D002460:Cell Line; D002884:Chromosomes, Human, Pair 15; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000302:isolation & purification*; D006801:Humans; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length
D004276:DNA, Satellite