title: Regulation of the acto.myosin subfragment 1 interaction by troponin/tropomyosin. Evidence for control of a specific isomerization between two acto.myosin subfragment 1 states.
abstract: The co-operative binding of myosin subfragment 1 (S1) to reconstituted skeletal-muscle thin filaments has been examined by monitoring the fluorescence of a pyrene probe on Cys-374 of actin. The degree of co-operativity differs when phosphate, sulphate or ADP are bound to the S1 active site. Binding isotherms have been analysed according to the Geeves & Halsall [(1987) Biophys. J. 52, 215-220] model, which proposed that troponin and tropomyosin effected regulation of the actomyosin interaction by controlling an isomerization of the actomyosin complex. The data support the proposal that seven actin monomers associated with a single tropomyosin molecule act as a co-operative unit that can be in one of two states. In the 'closed' state myosin can bind to actin, but the subsequent isomerization is prevented. The isomerization is only allowed after the seven-actin unit is in the 'open' form. Ca2+ controls the proportion of actin filaments in the 'closed' and 'open' forms in the absence of myosin heads. The ratio of 'closed' to 'open' forms is approx. 50:1 in the absence of Ca2+ and 5:1 in its presence.
authors.affiliation: Department of Biochemistry, School of Medical Sciences, University of Bristol, U.K.
authors.forename: D F
authors.initials: DF
authors.lastname: McKillop
authors.forename: M A
authors.initials: MA
authors.lastname: Geeves
mesh_terms: D000199:Actins / Q000737:chemistry; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D007536:Isomerism; D015879:Myosin Subfragments / Q000737:chemistry*; D010710:Phosphates; D011721:Pyrenes / Q000737:chemistry; D011817:Rabbits; D013431:Sulfates; D014335:Tropomyosin / Q000494:pharmacology*; D014336:Troponin / Q000494:pharmacology*
chemical_list: D000199:Actins; D015879:Myosin Subfragments; D010710:Phosphates; D011721:Pyrenes; D013431:Sulfates; D014335:Tropomyosin; D014336:Troponin; D000244:Adenosine Diphosphate; C030984:pyrene