T lymphocyte recognition of a celiac disease-associated cis- or trans-encoded HLA-DQ alpha/beta-heterodimer.
The HLA-associated susceptibility to develop celiac disease may to a large extent be attributed to the combination of particular DQA1 and DQB1 genes, i.e., the DQA1*0501 and DBQB1*0201 alleles, located either in cis position (on the DR3DQw2 haplotype) or in trans position (DR5DQw7/DR7DQw2 heterozygous individuals). We report three alloreactive T lymphocyte clones that recognize an HLA-DQ alpha/beta heterodimer both when the DQA1*0501 and DQB1*0201 alleles are located in cis or in trans position. Thus, the celiac disease associated DQA1 and DQB1 genes encode a functionally expressed DQ alpha/beta heterodimer.
Lundin
K E
KE
Institute of Transplantation Immunology, National Hospital, Oslo, Norway.
Sollid
L M
LM
Qvigstad
E
E
Markussen
G
G
Gjertsen
H A
HA
Ek
J
J
Thorsby
E
E
D000483:Alleles; D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002446:Celiac Disease / Q000276:immunology*; D002469:Cell Separation; D003001:Cloning, Molecular; D006683:HLA-DQ Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes
D000911:Antibodies, Monoclonal; D006683:HLA-DQ Antigens; D046911:Macromolecular Substances; D015345:Oligonucleotide Probes