title: [Gas chromatographic/mass spectrometric analysis of boldenone urinary metabolites in man].
abstract: The metabolism of boldenone (17 beta-hydroxy-1,4-androstem-3-one) in man has been investigated by gas chromatography/mass spectrometry. After oral administration of a 20 mg dose to man, six metabolites were detected in the conjugated fraction of the urinary samples. Boldenone, the major compound excreted in urine, was detected within 34 h after administration. In addition, several metabolites, resulting from the hydroxylation of boldenone and the reduction of the unsaturated carbon bonds of boldenone, were detected in the urine samples varying from 9 to 83 h. Extraction and fractionation of these metabolites were achieved by using XAD-2 column and gas chromatography. The recovery of the whole procedure was studied. Furthermore, the mass spectra of the metabolites are presented and major fragment pathways are discussed.
authors.affiliation: Department of Analytical Chemistry, Chinese Academy of Medical Sciences, Beijing.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: C S
authors.initials: CS
authors.lastname: Liu
authors.forename: T H
authors.initials: TH
authors.lastname: Zhou
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013739:Testosterone / Q000031:analogs & derivatives* / Q000652:urine
chemical_list: D013739:Testosterone; C013915:boldenone