Recombinants from a proviral bovine leukemia virus genome corresponding to the 3' region transactivate viral LTR in NIH3T3 and non-infected FLK cells.
Bovine leukemia virus (BLV), like human T-cell leukemia viruses, Types I and II, contains three open reading frames at the 3' end of its genome. The longest open reading frame encodes a transactivator protein which is generated by a doubly-spliced mRNA. A series of co-transfection experiments, using proviral BLV pX expression plasmids under the control of the Moloney leukemia virus LTR and the indicator plasmid containing the assayable lac Z gene under the control of BLV LTR, revealed that both NIH3T3 cells and non-infected fetal lamb kidney cells are able to express an active transactivator protein.
Noetzel
U
U
Central Institute of Molecular Biology, Academy of Sciences, Berlin-Buch.
Bauer
D
D
Fischer
U
U
Rosenthal
S
S
D000818:Animals; D002460:Cell Line; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D010957:Plasmids; D011533:Proviruses / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D015534:Trans-Activators / Q000235:genetics; D015533:Transcriptional Activation; D014162:Transfection
D015534:Trans-Activators