Evaluation of latex agglutination and dot immunobinding assay for the detection of cytomegalovirus antibody.
The Virogen CMV Antibody Test and Difco CMV-Cube were compared with three other available methods, enzyme immunoassay, immunofluorescence and latex agglutination, for the detection of cytomegalovirus (CMV) antibody in 126 random sera submitted to the clinical laboratory. Based on concordance of three or more methods, 72% of the samples were positive for CMV antibody. The sensitivities and specificities of the assays were Virogen, 98% and 97%, and CMV-Cube 97% and 97%, respectively.
Wilson
M T
MT
Microbiology Laboratory, Harrisburg Hospital, Pennsylvania.
Sautter
R L
RL
LeBar
W D
WD
Mervak
T R
TR
D000914:Antibodies, Viral / Q000097:blood*; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007841:Latex Fixation Tests; D011237:Predictive Value of Tests; D015203:Reproducibility of Results
D000914:Antibodies, Viral