title: Increased vitamin E intake restores fish-oil-induced suppressed blastogenesis of mitogen-stimulated T lymphocytes.
abstract: We sought to determine whether fish-oil supplementation would suppress blastogenesis in vitro of concanavalin A (ConA)-stimulated peripheral blood mononuclear cells (PBMCs) and, if so, whether it could be reversed with increased intake of vitamin E. Healthy males ate a controlled basal diet providing a total of 40% of energy from fat when fed in conjunction with 15 g/d of either placebo oil (PO) or fish-oil concentrate (FOC) fortified with 15 mg alpha-tocopherol/d for three periods. The subjects were supplemented with PO for 10 wk (PO), with FOC for 10 wk (FOC), and with FOC plus an additional 200 mg alpha-tocopherol/d for 8 wk (FOC+E). During FOC supplementation mitogenic responsiveness of PBMCs to ConA was suppressed, but this effect was reversed by concurrent supplementation with all-rac-alpha-tocopherol (FOC+E). There was a significant positive relationship (P less than 0.001) between plasma alpha-tocopherol concentrations and responsiveness of T lymphocytes to ConA.
authors.affiliation: Vitamin and Mineral Laboratory, US Department of Agriculture, Beltsville, MD 20705.
authors.forename: T R
authors.initials: TR
authors.lastname: Kramer
authors.forename: N
authors.initials: N
authors.lastname: Schoene
authors.forename: L W
authors.initials: LW
authors.lastname: Douglass
authors.forename: J T
authors.initials: JT
authors.lastname: Judd
authors.forename: R
authors.initials: R
authors.lastname: Ballard-Barbash
authors.forename: P R
authors.initials: PR
authors.lastname: Taylor
authors.forename: H N
authors.initials: HN
authors.lastname: Bhagavan
authors.forename: P P
authors.initials: PP
authors.lastname: Nair
mesh_terms: D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000494:pharmacology; D005395:Fish Oils / Q000494:pharmacology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D012044:Regression Analysis; D013601:T-Lymphocytes / Q000187:drug effects*; D014810:Vitamin E / Q000097:blood / Q000494:pharmacology*
chemical_list: D005395:Fish Oils; D003208:Concanavalin A; D014810:Vitamin E; D015118:Eicosapentaenoic Acid