Specific activation of B-cell clones within the central nervous system in course of herpes simplex encephalitis.
Total and virus-specific immunoglobulin (Ig) G oligoclonal bands were studied in paired serum and cerebrospinal fluid (CSF) of four patients with herpes simplex virus type 1 (HSV-1) encephalitis. We used the isoelectric focusing in agarose gel, a sensitive technique for protein separation, followed by passive transfer of proteins on nitrocellulose paper and specific immunostaining. Oligoclonal bands were observed in serum and CSF of all patients. HSV-1-specific oligoclonal IgG bands were present in the CSF only during a limited period of the disease, having their counterpart in serum during the remaining periods. Our findings contribute to tackle the issue of B-cell activation within central nervous system and peripheral blood compartments in course of HSV-1 encephalitis.
Ceroni
M
M
IRCCS Fondazione C. Mondino Università di Pavia, Sezione di Pavia.
Camana
C
C
Franciotta
D M
DM
Bolelli
G
G
Revello
M G
MG
Gerna
G
G
Savoldi
F
F
D000914:Antibodies, Viral / Q000134:cerebrospinal fluid / Q000276:immunology; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology / Q000473:pathology; D004660:Encephalitis / Q000134:cerebrospinal fluid / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D006561:Herpes Simplex / Q000134:cerebrospinal fluid / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000134:cerebrospinal fluid; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000134:cerebrospinal fluid; D008213:Lymphocyte Activation; D018139:Simplexvirus / Q000276:immunology
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M