An ELISA for the diagnosis of Babesia ovis infection utilizing a synthetic, Babesia bovis-derived antigen.
The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of Babesia ovis antibodies is described. In an initial study, a crude Babesia bovis antigen and a synthetic B. bovis-derived antigen (designated 11C5) were used to screen 46 B. ovis-positive and 55 negative sheep sera. A 95% correlation between the two antigenic preparations was found with the positive sera; no negative sera gave positive reactions. The synthetic antigen was then used in the screening of 1466 sera collected from sheep from 18 regions of Turkey. A high incidence of B. ovis-positive reactions was found from all regions (60-80%) in sheep over 1 year old, while from two smaller samples the incidence in young sheep was much less (28 and 52%). This test is superior to existing ones because the synthetic antigen can be produced in a highly reproducible state, is specific and is stable over extended periods of time.
Duzgun
A
A
Lalahan Nuclear Research Institute of Animal Science, Lalahan-Ankara, Turkey.
Wright
I G
IG
Waltisbuhl
D J
DJ
Gale
K R
KR
Goodger
B V
BV
Dargie
J D
JD
Alabay
M
M
Cerci
H
H
D000818:Animals; D000953:Antigens, Protozoan; D016793:Babesia bovis / Q000276:immunology*; D001404:Babesiosis / Q000175:diagnosis*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D011237:Predictive Value of Tests; D011994:Recombinant Proteins; D015203:Reproducibility of Results; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*; D014421:Turkey
D000953:Antigens, Protozoan; D011994:Recombinant Proteins