The fimD gene required for cell surface localization of Escherichia coli type 1 fimbriae.
The fimD gene of Escherichia coli K12 was shown to be necessary for surface localization of type 1 fimbriae, since deletion of the gene resulted in a virtually bald phenotype. The FimD protein was found to be located in the outer membrane. Expressed alone, this protein had a very deleterious effect on cell growth. The DNA sequence of the fimD gene was determined; the corresponding amino acid sequence of the FimD protein was compared with those of the PapC and FaeD proteins. A deletion derivative of FimD gave clues as to which parts of the protein were necessary for outer membrane integration.
Klemm
P
P
Department of Microbiology, Technical University of Denmark, Lyngby.
Christiansen
G
G
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002462:Cell Membrane / Q000032:analysis / Q000648:ultrastructure; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D010861:Fimbriae, Bacterial / Q000032:analysis* / Q000648:ultrastructure; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010641:Phenotype; D015183:Restriction Mapping
D001426:Bacterial Proteins