[Quantitative analysis of midecamycin by HPLC].
A reversed phase high performance liquid chromatographic method for the assay of midecamycin was developed. The method used a Hitachi Gel 3050 column at 50 degrees C and a mobile phase of methanol--0.01 mol/L phosphate buffer solution at pH 5.8 (45:55). The column effluent was monitored Z at 231 nm. SF-837A1, leucomycin A6 and minor components could be separated in less than 15 min. The retention times of SF-837A1 and leucomycin A6 are 7 and 10 min respectively. The method is very simple and rapid.
He
H
H
China Pharmaceutical University, Nanjing.
Yu
R G
RG
Yang
Q H
QH
Yu
Z D
ZD
Wang
X H
XH
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007933:Leucomycins / Q000032:analysis / Q000737:chemistry*; D011786:Quality Control
D007933:Leucomycins; C071430:leucomycin A6; C026483:midecamycin