Cleavage reaction of a synthetic oligoribonucleotide corresponding to the autocleavage site of a precursor RNA from bacteriophage T4.
A fragment (GUUUCGUACAAAC) having a consensus sequence for the self-cleavage domain in a precursor of an RNA molecule from T4-infected Escherichia coli cells (p2Sp1; precursor of species 1) was chemically synthesized and found to be cleaved either between CA (139-140) or between UA (137-138) in the presence of monovalent cations and a non-ionic detergent. The cleaved products had 5'-hydroxyl and 3'-phosphate groups, of which some were in the form 2',3'-cyclic phosphates.
Hosaka
H
H
Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
Ogawa
T
T
Sakamoto
K
K
Yokoyama
S
S
Takaku
H
H
D001483:Base Sequence; D003902:Detergents / Q000494:pharmacology; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D012322:RNA Precursors / Q000737:chemistry*; D012367:RNA, Viral / Q000737:chemistry*; D013604:T-Phages / Q000235:genetics*
D003902:Detergents; D009843:Oligoribonucleotides; D012322:RNA Precursors; D012367:RNA, Viral