Anthracycline metabolites from Streptomyces violaceus A262. I. Isolation of antibiotic-blocked mutants from Streptomyces violaceus A262.
Five mutant (or variant) strains producing new anthracycline antibiotics were derived from Streptomyces violaceus A262 by mutagenesis treatment. Strain SE1-625 showed a limited production of three known beta-rhodomycinone diglycosides while the parent strain produced numerous unidentified beta-rhodomycinone glycosides. Strain SU2-730 was an antibiotic-blocked mutant which produced only epsilon-rhodomycinone glycosides (named epelmycins). Strains SC-7 and SE2-2385 were variants which produced alpha-citromycinone glycosides (named yellamycins) and beta-isorhodomycinone glycosides (named obelmycins), respectively. Strain SE2-2385-A1 produced alpha 2-rhodomycinone glycosides (named alldimycins). Glycosidation-less mutants which accumulated only aglycone were also obtained. Isolation of these mutants or variants and preliminary identification of their anthracycline products are described.
Johdo
O
O
Central Research Laboratories, Mercian Corporation, Fujisawa, Japan.
Ishikura
T
T
Yoshimoto
A
A
Takeuchi
T
T
D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D002855:Chromatography, Thin Layer; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D016296:Mutagenesis; D009604:Nitrosoguanidines / Q000494:pharmacology; D013302:Streptomyces / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism* / Q000528:radiation effects
D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic; D009604:Nitrosoguanidines; C015490:rhodomycinone; D005020:Ethyl Methanesulfonate