title: Location of a mutation in the aspartyl-tRNA synthetase gene of Escherichia coli K12.
abstract: A mutation (tls-1) that confers a temperature-sensitive growth phenotype in Escherichia coli was shown by DNA cloning and sequencing to be an allele of aspS, the gene for aspartyl-tRNA synthetase. The mutation, which lies near minute 41 on the genetic map, was located some 2.3 kb from the 5' end of the ruvAB operon. A DNA fragment encoding the carboxy-terminus of AspRS was found to be sufficient to allow growth of a tls-1 strain at the non-permissive temperature.
authors.affiliation: Department of Genetics, University of Nottingham, Medical School, Queens Medical Centre, Great Britain.
authors.forename: G J
authors.initials: GJ
authors.lastname: Sharples
authors.forename: R G
authors.initials: RG
authors.lastname: Lloyd
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001226:Aspartate-tRNA Ligase / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial; D001226:Aspartate-tRNA Ligase