Secretions of somatostatin and VIP in cultures of fetal rat neuroblasts increased by amino acids.
Neuroblasts obtained from 17 day old rat embryos were incubated for 8 days, after which half of them were treated with 10(-6) M FACE (a mixture of amino acids high in glycine, alanine and aspartic acid), and the other half were left as controls. At the end of 20 days, levels of somatostatin (SRIF) were over 6,000 pg/plate in neuroblasts treated with FACE, versus 500 pg/plate in controls. At this time vasoactive intestinal peptide (VIP) levels were over 230 pg/plate in the FACE treated cultures, while their controls contained less than 150 pp/plate. Protein totals were similar (about 1,000 micrograms/plate) in all FACE treated cultures and controls, indicating that increases in SRIF and VIP were not determined by changes in cell population, but by their synthetic and/or secretory activities triggered by minute amounts of FACE. These results may be of interest in the understanding of Alzheimer's disease.
Delgado
J M
JM
LBE-Pharma, Madrid, Spain.
Cacicedo
L
L
Lorenzo
M J
MJ
de los Frailes
M T
MT
Sanchez-Franco
F
F
D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D011506:Proteins / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000096:biosynthesis*; D014660:Vasoactive Intestinal Peptide / Q000096:biosynthesis*
D000596:Amino Acids; D011506:Proteins; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin