title: [In situ hybridization study of human papillomavirus from the penile cancer].
abstract: The histochemical detection of human papillomavirus (HPV) was performed using the technique of in situ hybridization from the formalin fixed paraffin embedded specimens of penile carcinoma and condylomata+ acuminata . One of 19 penile cancer cases (5.3%) revealed the positive staining on the nuclei of the tumors for HPV type 16/18. However, the positive nuclei were scattered and localized in small part of the tumor which showed no koilocytosis. On the contrary, 11 of 12 condyloma acuminata cases (91.7%) showed positive staining on the nuclei of the tumors for HPV type 6/11, also 5 of the 11 cases (45.5%) revealed cross reaction for other type of HPV. The HPV types 6/11, 16/18 and 31/33/35 may be unrelated to the pathogenesis of the Japanese penile carcinoma. However, further study is necessary to evaluate that the number of HPV-DNA copy in our cases is too small, or the other type of HPV is responsibility.
authors.affiliation: Department of Urology, Branch Hospital, Faculty of Medicine, University of Tokyo.
authors.forename: N
authors.initials: N
authors.lastname: Moriyama
authors.forename: Y
authors.initials: Y
authors.lastname: Nagase
authors.forename: T
authors.initials: T
authors.lastname: Ueki
authors.forename: Y
authors.initials: Y
authors.lastname: Hosaka
authors.forename: E
authors.initials: E
authors.lastname: Higashihara
authors.forename: I
authors.initials: I
authors.lastname: Murahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Aso
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003218:Condylomata Acuminata / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000302:isolation & purification* / Q000472:pathogenicity; D010412:Penile Neoplasms / Q000382:microbiology*; D014412:Tumor Virus Infections / Q000382:microbiology*
chemical_list: D004279:DNA, Viral