B-B cell interaction involved in polyclonal B cell activation is restricted by I-A but not by I-E molecules.
We have previously demonstrated that the class II MHC restricted B-B cell interaction is involved in the polyclonal differentiation of unprimed murine B cells into IgM-producing cells induced by a T cell-derived lymphokine B151-TRF2 or bacterial LPS. The present study has addressed the question of whether I-A and/or I-E molecules function as restriction elements for the B-B cell interaction. The results revealed that (B10 x B10.BR)F1(H-2b/k) B cells could be separated into I-Ab- and 1-Ak-restricted subpopulations by their ability to bind to B10(H-2b) or B10.BR(H-2k) B cell monolayers, whereas an I-E-restricted F1 B cell population was not obtained. Moreover, B10-derived B cells isolated from (B10 + B10.BR) - (B10 x B10.BR)F1 but not from B10 - (B10 x B10.BR)F1 radiation-induced bone marrow chimeras acquired newly the ability to co-operate with mitomycin C-treated auxilary B cells expressing I-Ak but not I-Ek molecules. Thus, these results indicate that I-E molecules, unlike I-A molecules, do not serve as restriction elements for the B-B cell interaction, and that I-A and I-E molecules on B cells play functionally disparate roles in the activation of polyclonal B cells.
Takahama
Y
Y
Biomedical Research Center, Osaka University Medical School, Japan.
Ono
S
S
Ishihara
K
K
Hirano
H
H
Hamaoka
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002450:Cell Communication; D002454:Cell Differentiation; D006183:H-2 Antigens / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007075:Immunoglobulin M / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008222:Lymphokines / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology; D011828:Radiation Chimera
D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D008222:Lymphokines