title: Murine B cells expressing Thy-1 after in vivo immunization selectively secrete IgE.
abstract: IL-4 induces Thy-1 expression and IgE secretion by LPS--and T cell-stimulated murine B cells in vitro. IFN-gamma inhibits both of these IL-4-mediated effects. IL-4 and IFN-gamma are often exclusively produced by different CD4+ T cell subsets. Injection of mice with a polyclonal goat anti-mouse IgD antibody (GaM delta) stimulates large increases in the serum concentration of IgE through the production of IL-4. Neutralization of endogenous IFN-gamma production in GaM delta-injected mice leads to further increases in serum IgE levels. We show that IL-4 and IFN-gamma, produced after GaM delta injection, respectively, stimulate and inhibit the number of splenic B cells expressing Thy-1 in vivo. Increased numbers of Thy-1-expressing B cells are observed concomitantly with the onset of enhanced IgE secretion. These Thy-1-expressing B cells are highly and selectively enriched for IgE-secreting cells.
authors.affiliation: Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814.
authors.forename: C M
authors.initials: CM
authors.lastname: Snapper
authors.forename: J J
authors.initials: JJ
authors.lastname: Hooley
authors.forename: S
authors.initials: S
authors.lastname: Barbieri
authors.forename: F D
authors.initials: FD
authors.lastname: Finkelman
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000008:administration & dosage; D000954:Antigens, Surface / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D005434:Flow Cytometry; D007073:Immunoglobulin E / Q000378:metabolism*; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis; D007371:Interferon-gamma / Q000502:physiology; D015847:Interleukin-4 / Q000502:physiology; D051379:Mice; D008815:Mice, Inbred Strains; D018800:Thy-1 Antigens; D013997:Time Factors
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000954:Antigens, Surface; D007132:Immunoglobulin Isotypes; D018800:Thy-1 Antigens; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma