Histiocytic conversion of human adult skin fibroblasts by the Snyder-Theilen feline sarcoma virus.
Oncogenes are apparently involved in the transformation/neodifferentiation of human cells. We now report a novel example of transformation/neodifferentiation: the specific conversion of cultured human adult skin fibroblasts (HSF) to histiocytes (tissue macrophages (TM)) by the Snyder-Theilen feline sarcoma virus (ST: (FeSV)). The de novo conversion of cultured HSF was demonstrated in a large fraction of ST:FeSV (FeLV)-transformed foci in the presence of dexamethasone (DX). Identification of tissue macrophages in ST:FeSV(FeLV)-infected HSF cultures was established by light-and transmission electron microscopy, histochemistry, immunohistochemistry, adherence-reattachment, latex particle uptake, and secretion of bioactive interleukin-1. The ST:FeSV gene and glucocorticosteroids, or other naturally occurring hormones, may play a role in morphogenetic processes within cells from a variety of normal and diseased tissues in situ, including induction of non-bone marrow, mesenchyme-derived, tissue macrophages.
Kopelovich
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Laboratory of Cancer Genetics and Cancer Prevention, VAMC, Bay Pines, Fl.
Wang
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D000328:Adult; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D015415:Biomarkers; D002454:Cell Differentiation; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003673:Defective Viruses / Q000502:physiology; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts / Q000382:microbiology; D006378:Helper Viruses / Q000502:physiology; D006644:Histiocytes / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D051379:Mice; D008809:Mice, Inbred C3H; D008854:Microscopy, Electron; D012190:Retroviridae / Q000502:physiology; D005253:Sarcoma Viruses, Feline / Q000502:physiology*; D012867:Skin / Q000166:cytology*
D000954:Antigens, Surface; D015415:Biomarkers; D007375:Interleukin-1; D003907:Dexamethasone