Immobilization of penicillin G acylase onto ion exchange and hydrophobic resins.
Penicillin G acylase was immobilized on numerous cation exchange resins and hydrophobic adsorbents. Amberlite XAD-7 was the matrix of choice among the matrices studied for the immobilization of enzyme. Binding of 96.8% and expression of 82.6% of the penicillin G acylase was achieved on XAD-7. Penicillin G acylase immobilised on XAD-7 was used for 80 cycles for the production of 6-PA in a stirred tank reactor.
Kumar
K K
KK
Hindustan Antibiotics Ltd., Pimpri, Pune.
Ambekar
G R
GR
Shewale
J G
JG
D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D010397:Penicillanic Acid / Q000378:metabolism* / Q000031:analogs & derivatives; D010399:Penicillin Amidase; D011137:Polystyrenes
D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D011137:Polystyrenes; C015661:amberlite XAD 7; D010397:Penicillanic Acid; D010399:Penicillin Amidase; C004408:aminopenicillanic acid