title: Size separation of polystyrene sulfate particles (189 to 1085 nm radius) in solutions of methyl-hydroxypropyl-cellulose of different chain lengths.
abstract: Polystyrene sulfate particles of 189 to 1,085 nm radius are retarded in their electrophoretic migration in solutions of methyl-hydroxypropyl-cellulose (4,000 and 15,000 cP viscosity for a 2% solution). The retardation is proportional to polymer concentration and increases inversely with particle size. This suggests a separation mechanism based on size-dependent exclusion from the polymer network, as found previously in other liquid polymer media. In the size range of approximately 200 to 600 nm radius, the 4,000 cP polymer exhibits a steeper decrease of retardation with particle size than the 15,000 cP polymer, but fails to exhibit any size dependent retardation beyond that range.
authors.affiliation: Section on Macromolecular Analysis, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: J A
authors.initials: JA
authors.lastname: Burroughs
authors.forename: A
authors.initials: A
authors.lastname: Chrambach
mesh_terms: D002793:Cholic Acids; D004247:DNA / Q000302:isolation & purification*; D065347:Hypromellose Derivatives; D008747:Methylcellulose / Q000031:analogs & derivatives*; D008968:Molecular Conformation; D008970:Molecular Weight; D011137:Polystyrenes / Q000302:isolation & purification*; D012984:Software; D012996:Solutions
chemical_list: D002793:Cholic Acids; D011137:Polystyrenes; D012996:Solutions; C071389:polystyrene sulfate; D065347:Hypromellose Derivatives; D008747:Methylcellulose; D004247:DNA; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate