title: Ototoxic effect of potassium canrenoate on the guinea pig cochlea.
abstract: Potassium canrenoate (PC) is a diuretic with antialdosterone action, reducing the reabsorption of sodium in the efferent renal tubules. This drug has been reported to damage only the marginal cells of the stria vascularis in the cochlea. The perilymphatic space of the guinea pig cochlea was perfused with an artificial perilymph containing 5 x 10(-3) M potassium canrenoate. Following the onset of perfusion, the endocochlear dc potential (EP) gradually declined to around 10 mV but did not become negative even when the perfusion was continued. A similar decrease in EP was observed in kanamycin-deafened guinea pigs. When the respirator was turned off and perfusion discontinued, a large negative EP appeared during 5 min of anoxia in normal guinea pigs but not in the kanamycin-deafened guinea pigs. The decreased EP recovered to preanoxic level after resumption of ventilation. The cochlear microphonics (CM) also gradually declined in parallel with the EP. The summating potential (SP) showed only a minor change. The potassium ion activity in the endolymph decreased slightly but the sodium ion activity remained unchanged during perfusion. These findings suggest that the main target of the PC is the cells of the stria vascularis.
authors.affiliation: Department of Otorhinolaryngology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Komune
authors.forename: S
authors.initials: S
authors.lastname: Wakizono
authors.forename: T
authors.initials: T
authors.lastname: Nakagawa
authors.forename: T
authors.initials: T
authors.lastname: Kimituki
authors.forename: K
authors.initials: K
authors.lastname: Hisashi
authors.forename: T
authors.initials: T
authors.lastname: Uemura
mesh_terms: D000818:Animals; D002191:Canrenoic Acid / Q000633:toxicity*; D003051:Cochlea / Q000187:drug effects*; D003055:Cochlear Microphonic Potentials / Q000187:drug effects; D004710:Endolymph / Q000187:drug effects / Q000378:metabolism; D005072:Evoked Potentials, Auditory / Q000187:drug effects*; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000187:drug effects / Q000648:ultrastructure; D008854:Microscopy, Electron; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013316:Stria Vascularis / Q000648:ultrastructure
chemical_list: D002191:Canrenoic Acid; D012964:Sodium; D011188:Potassium