Neuronal markers in rat retinal grafts.
Rat E15 retina was grafted to the retina of adult rat hosts. After varying survival times (1 week-6 months), grafts were stained by immunohistochemistry for neurofilament 160 kDa (NF), HPC-1 (an amacrine cell marker), choline acetyltransferase (ChAT), tyrosine hydroxylase (TH), glutamic acid decarboxylase (GAD) and somatostatin-28 (SS-28). The first differentiating graft amacrine cells (cholinergic and dopaminergic) could be seen 1 week after transplantation (corresponding to postnatal day 1 = P1). The inner plexiform layer of the graft started to differentiate at 2 weeks (corresponding to P8) seen by HPC-1 and GAD staining. ChAT, TH and SS-28 immunostaining revealed an abnormal lamination pattern in the graft inner plexiform layer. Also by 2 weeks, the outer plexiform layers of the graft contained NF-immunoreactive horizontal cells. No NF-stained retinal ganglion cells could be observed in the graft. Five and 7 weeks after grafting, the transplants had obtained the same staining intensity with different markers as the host retina.
Aramant
R
R
Eye Research Institute of Retina Foundation, Boston, MA 02114.
Seiler
M
M
Ehinger
B
B
Bergström
A
A
Adolph
A R
AR
Turner
J E
JE
D000818:Animals; D002454:Cell Differentiation; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D002799:Cholinergic Fibers / Q000201:enzymology / Q000502:physiology*; D004298:Dopamine / Q000378:metabolism / Q000502:physiology*; D005968:Glutamate Decarboxylase / Q000378:metabolism; D006085:Graft Survival; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D008297:Male; D016900:Neurofilament Proteins; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D013004:Somatostatin / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism / Q000502:physiology*
D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D013004:Somatostatin; D005680:gamma-Aminobutyric Acid; D014446:Tyrosine 3-Monooxygenase; D002795:Choline O-Acetyltransferase; D005968:Glutamate Decarboxylase; D004298:Dopamine