title: Binding of simian virus 40 large tumor antigen to phospholipid vesicles.
abstract: SV40 T antigen is the initiator protein of SV40 DNA replication. We examined the interaction of purified SV40 T antigen with phospholipids by (i) centrifugation analysis with phospholipid vesicles, (ii) filter binding assay and footprint analysis of T antigen binding to the replication origin of SV40 DNA and (iii) analysis of the initiation of SV40 DNA replication in vitro. In all cases, cardiolipin showed affinity for T antigen and inhibited its DNA binding capacity. Phosphatidylglycerol with unsaturated fatty acids also inhibited the binding of T antigen to the replication origin of SV40 DNA, whereas phosphatidylglycerol with saturated fatty acids did not. This finding suggested the importance of unsaturated fatty acids for the interaction of T antigen with phospholipids. Other phospholipids including phosphatidylserine, phosphatidylinositol and phosphatidylethanolamine showed little or no affinity for T antigen.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hirai
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
authors.forename: S
authors.initials: S
authors.lastname: Natori
authors.forename: K
authors.initials: K
authors.lastname: Sekimizu
mesh_terms: D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism*; D002498:Centrifugation; D004261:DNA Replication; D004279:DNA, Viral; D007700:Kinetics; D010743:Phospholipids / Q000378:metabolism*
chemical_list: D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D010743:Phospholipids