Measurement of cytokine production by the monocytic cell line Mono Mac 6 using novel immunoradiometric assays for interleukin-1 beta and interleukin-6.
Immunoradiometric assays for interleukin-1 beta and interleukin-6 were developed using affinity-purified IgG fractions from antisera initially raised for radioimmunoassay. Capture and detector functions were carried out by the same antibody preparation within each assay. The assays were precise, rapid and 6-8-fold more sensitive than the RIA systems previously employed. IRMAs were used for the initial characterisation of a candidate cell line (Mono Mac 6), under investigation in a 'monocyte test' for pyrogen detection, and permitted rapid and specific determination of the cytokines under stimulatory and inhibitory conditions.
Rafferty
B
B
Division of Endocrinology, National Institute for Biological Standards and Control, Potters Bar, Herts, U.K.
Mower
J A
JA
Taktak
Y S
YS
Poole
S
S
D000818:Animals; D003429:Cross Reactions; D004731:Endotoxins / Q000494:pharmacology; D006041:Goats; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007375:Interleukin-1 / Q000032:analysis* / Q000096:biosynthesis; D015850:Interleukin-6 / Q000032:analysis* / Q000096:biosynthesis; D007948:Leukemia, Monocytic, Acute / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism*; D011863:Radioimmunoassay; D012756:Sheep; D014407:Tumor Cells, Cultured
D004731:Endotoxins; D007375:Interleukin-1; D015850:Interleukin-6