Modulation by prostaglandin E2 of ATP and noradrenaline co-transmission in the guinea-pig vas deferens.
1. In the guinea-pig vas deferens, prostaglandin E2 (100 nM) enhanced the overflow of ATP, whereas it inhibited [3H]-noradrenaline overflow due to field stimulation at 2 Hz. At 20 Hz, prostaglandin E2 still inhibited the overflow of [3H]-noradrenaline, whereas it was without effect on ATP overflow. 2. Prostaglandin E2 enhanced contractions to exogenously added noradrenaline and alpha, beta-methylene ATP. 3. These results provide evidence for pre- and postjunctional modulation of purinergic and adrenergic transmission by PGE2 in the guinea-pig vas deferens. The importance of these findings in relation to co-transmission is discussed.
Ellis
J L
JL
Department of Anatomy and Developmental Biology, University College London, UK.
Burnstock
G
G
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D015232:Dinoprostone / Q000494:pharmacology*; D004558:Electric Stimulation; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D011941:Receptors, Adrenergic / Q000502:physiology; D011983:Receptors, Purinergic / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects* / Q000502:physiology; D014316:Tritium; D014649:Vas Deferens / Q000187:drug effects / Q000294:innervation*
D011941:Receptors, Adrenergic; D011983:Receptors, Purinergic; D014316:Tritium; D000255:Adenosine Triphosphate; D015232:Dinoprostone; C002630:alpha,beta-methyleneadenosine 5'-triphosphate; D009638:Norepinephrine