title: Detection of human cytomegalovirus in cervicovaginal cells by culture, in situ DNA hybridization and DNA amplification methods.
abstract: The presence of human cytomegalovirus (HCMV) was tested in 388 cervicovaginal cells specimens obtained from the same number of pregnant women. HCMV was detected in 5.41%, 11.6% and 13.9% of these specimens by conventional culture, in situ DNA hybridization and polymerase chain reaction (PCR) methods, respectively. The sensitivities of detecting HCMV by in situ hybridization and PCR methods were 76.2% and 90.5% and the specificities were 92.1% and 90.5%, respectively, when compared with conventional culture method. The PCR compared favourably with both conventional culture and in situ hybridization methods and it may become a valuable and useful tool for the early and rapid detection of HCMV in clinical specimens.
authors.affiliation: Department of Obstetrics and Gynecology, Veteran General Hospital, Taipei, Taiwan, Republic of China.
authors.forename: C F
authors.initials: CF
authors.lastname: Yuan
authors.forename: S M
authors.initials: SM
authors.lastname: Kao
authors.forename: D C
authors.initials: DC
authors.lastname: Wang
authors.forename: H T
authors.initials: HT
authors.lastname: Ng
authors.forename: C C
authors.initials: CC
authors.lastname: Pao
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000166:cytology / Q000382:microbiology*; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D014621:Vagina / Q000166:cytology / Q000382:microbiology*
chemical_list: D004279:DNA, Viral