Recombinant IL-2 therapy reverses diminished granulomatous responsiveness in anti-L3T4-treated, Schistosoma mansoni-infected mice.
We have previously shown, that anti-L3T4 mAb treatment strongly suppressed granuloma formation in the liver, and IL-2 production in the spleen of Schistosoma mansoni-infected mice. In the present study the dynamics of IL-2 production was delineated during the infection, and the effect of rIL-2 treatment on granulomatous responsiveness was examined. IL-2 production in soluble egg Ag-stimulated spleen cells of mice was detectable at 6, peaked at 8 and waned by 20 wk of the infection. In contrast, Con A stimulus elicited high levels of IL-2 production by 8 wk which remained nearly unchanged throughout the infection. Administration of rIL-2 to acutely infected, anti-L3T4 mAb-treated, or chronically infected mice reversed the diminished or modulated granulomatous responses without restoring the ability for endogenous IL-2 production. Transfer of spleen cells of anti-L3T4 mAb-treated, chronically infected mice did not indicate a role for Ts cells in the impaired production of IL-2 in recipients. These data suggest that lack of IL-2 production can play an important role in the immunoregulation of the granulomatous response.
Mathew
R C
RC
Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI 48201.
Ragheb
S
S
Boros
D L
DL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D015704:CD4 Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D006099:Granuloma / Q000473:pathology / Q000517:prevention & control; D007167:Immunotherapy; D007376:Interleukin-2 / Q000096:biosynthesis / Q000627:therapeutic use*; D051379:Mice; D008815:Mice, Inbred Strains; D011994:Recombinant Proteins; D012555:Schistosomiasis mansoni / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D013154:Spleen / Q000503:physiopathology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D007376:Interleukin-2; D011994:Recombinant Proteins