Radiofrequency dielectric spectroscopy of ribosome suspensions.
Dielectric measurements on different ribosome suspensions were carried out in the frequency range from 10 kHz to 1 GHz. In intact ribosomes two dispersions were detected: one around 100 kHz and the other one in the MHz region. In separated ribosomal subunits and in ribosomes resuspended in a buffer with no magnesium ions (relaxed ribosomes) only the MHz dispersion was observed. Electrical conductivities of the samples at 1 kHz were also measured. The temperature dependence of the two dispersions was investigated and a tentative attribution was proposed.
Bonincontro
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Dipartimento di Fisica, Università degli Studi di Roma, La Sapienza, Italy.
Giansanti
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Pedone
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Risuleo
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D004553:Electric Conductivity; D004926:Escherichia coli / Q000737:chemistry; D012270:Ribosomes / Q000737:chemistry* / Q000502:physiology; D013053:Spectrophotometry / Q000379:methods; D013535:Suspensions
D013535:Suspensions