Chemiluminescent substrates increase sensitivity of antigen detection in western blots.
Replacement of colorimetric substrates in Western blots by chemiluminescent reagents enhances the sensitivity of detection by more than an order of magnitude. Protein levels beyond the detection limit of colorimetric substrates can be consistently detected without modifying pre-existing laboratory protocols.
Sandhu
G S
GS
Dept. of Biochemistry and Molecular Biology, Mayo Clinic and Foundation, Rochester, MN 55905.
Eckloff
B W
BW
Kline
B C
BC
D000942:Antigens, Bacterial / Q000032:analysis*; D015153:Blotting, Western / Q000379:methods*; D003124:Colorimetry; D004926:Escherichia coli / Q000276:immunology*; D007211:Indoles; D008163:Luminescent Measurements; D009580:Nitroblue Tetrazolium
D000942:Antigens, Bacterial; D007211:Indoles; D009580:Nitroblue Tetrazolium; C035455:5-bromo-4-chloro-3-indoxyl phosphate