In vitro immunosuppressive effect: a comparative study between deoxymethylspergualin and cyclosporin A, on human lymphocyte responses.
The immunosuppressive mechanism of Deoxymethylspergualin (MeDSG) on human lymphocyte responses in vitro was assessed in comparison with Cyclosporin A (CyA). Peripheral blood mononuclear cells from normal human volunteers were used for assay of mixed lymphocyte reaction (MLR), cell-mediated lympholysis (CML) and blastogenesis by PHA and OKT3. MeDSG suppressed only allogenic stimulation (MLR and CML), however, CyA showed a suppressive effect for all assays. Kinetic study of MLR showed that the suppressive activity did not decrease even when MeDSG was added at day 3 or day 4. CyA, however, showed a weak suppressive effect when added at a later phase of MLR. The results suggest that MeDSG is a useful immunosuppressive agent and, in vitro, its immunosuppressive mechanism on human lymphocyte responses is quite different from CyA.
Jiang
H
H
Department of Urology, Osaka University Medical School, Japan.
Takahara
S
S
Kokado
Y
Y
Ishibashi
M
M
Sonoda
T
T
D016572:Cyclosporine / Q000494:pharmacology*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006146:Guanidines / Q000494:pharmacology*; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D015375:Receptors, Interleukin-2 / Q000378:metabolism
D006146:Guanidines; D007166:Immunosuppressive Agents; D015375:Receptors, Interleukin-2; D016572:Cyclosporine; C059810:15-deoxymethylspergualin