title: Effect of spermatozoa selection on a simplified Percoll gradient in case of asthenozoospermia.
abstract: Two methods for preparing semen (Swim-up migration and centrifugation on a two step density Percoll gradient) were tested in 21 cases of asthenozoospermia (motility less than 30%). The parameters of sperm movement were studied through automated videomicrographic analysis. In 9 cases out of 21 swim-up migration failed while centrifugation on a Percoll gradient failed in only 1 case out of 21.
authors.affiliation: Laboratoire de Biologie de la Reproduction, Faculté de Médecine, Lyon/France.
authors.forename: C
authors.initials: C
authors.lastname: Mathieu
authors.forename: M
authors.initials: M
authors.lastname: Mein
authors.forename: J
authors.initials: J
authors.lastname: Lornage
authors.forename: J G
authors.initials: JG
authors.lastname: Li
authors.forename: J F
authors.initials: JF
authors.lastname: Guerin
mesh_terms: D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D007317:Insemination, Artificial, Homologous / Q000379:methods*; D008297:Male; D011205:Povidone; D012822:Silicon Dioxide; D013081:Sperm Motility; D013094:Spermatozoa / Q000473:pathology
chemical_list: C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone