title: "Matrix effects" in immunoassays.
abstract: This article deals with "matrix effects" in immunoassay systems. As each component has its own matrix (buffer, antibody, separation agents), the term matrix effect--usually only referring to the matrix of the sample--has been replaced by the term interference effect. The possible sites of interference have been dealt with in turn, taking each component/step of an immunoassay separately. Results of assay improvement due to the removal of "matrix effects" are given using the PTH-assay as example. The change in results due to tampering with the serum matrix is given using the determination of free thyroxine in commercial quality control sera.
authors.affiliation: Dept. of Internal Medicine, Medical University of Lübeck, DE.
authors.forename: W G
authors.initials: WG
authors.lastname: Wood
mesh_terms: D000906:Antibodies; D002021:Buffers; D006801:Humans; D007118:Immunoassay / Q000379:methods / Q000592:standards* / Q000706:statistics & numerical data; D010281:Parathyroid Hormone / Q000097:blood / Q000592:standards; D012015:Reference Standards; D013972:Thyrotropin / Q000097:blood / Q000592:standards
chemical_list: D000906:Antibodies; D002021:Buffers; D010281:Parathyroid Hormone; D013972:Thyrotropin