Synthesis of 6 beta-hydroxyaldosterone by A6 (toad kidney) cells in culture.
Incubation of aldosterone with confluent layers of A6 (toad kidney) cells leads to its hydroxylation at the 6 beta-position. 6 beta-Hydroxyaldosterone is the major metabolite when the incubation is carried out at pH 6.8, whereas the product comprises 6 beta-hydroxy-17-isoaldosterone accompanied by some 6 beta-hydroxyapoaldosterone at pH 7.4. All products were identified by high-field 1H nuclear magnetic resonance spectroscopy. Control experiments indicated that the side-chain isomerization to form the 17-iso and apo derivatives occurs after the cytochrome P 450-dependent synthesis of 6 beta-hydroxyaldosterone.
Morris
D J
DJ
Department of Laboratory Medicine, Miriam Hospital, Providence, RI 02906.
Latif
S A
SA
Conca
T J
TJ
Wei
L T
LT
Watlington
C O
CO
Kirk
D N
DN
Toms
H C
HC
Shackleton
C H
CH
D000450:Aldosterone / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System; D008401:Gas Chromatography-Mass Spectrometry; D007668:Kidney / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011522:Protons; D013250:Steroid Hydroxylases / Q000378:metabolism*; D013379:Substrate Specificity; D014981:Xenopus
D011522:Protons; C067782:6-hydroxyaldosterone; D000450:Aldosterone; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; C022657:steroid hormone 6-beta-hydroxylase