title: c-myc detection in bone marrow biopsies.
abstract: In the past the fixation procedures employed in the immunohistostaining of paraffin-embedded tissues with anti-myc protein antibodies were unsatisfactory since they permitted the leakage of the protein from its normal nuclear localization in the cell. In the studies described here 3 different fixation methods were compared and it was shown that only one, the AMeX method, was suitable for studying c-myc expression in bone marrow biopsies.
authors.affiliation: Department of Experimental Biology and Medicine, Rudjer Boskovic Institute, Zagreb, Yugoslavia.
authors.forename: K
authors.initials: K
authors.lastname: Pavelic
authors.forename: Z P
authors.initials: ZP
authors.lastname: Pavelic
authors.forename: H D
authors.initials: HD
authors.lastname: Preisler
mesh_terms: D000096:Acetone; D001853:Bone Marrow / Q000648:ultrastructure*; D005189:False Positive Reactions; D005404:Fixatives; D005557:Formaldehyde; D015870:Gene Expression; D016259:Genes, myc; D006801:Humans; D007150:Immunohistochemistry; D011108:Polymers; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis
chemical_list: D005404:Fixatives; D011108:Polymers; D016271:Proto-Oncogene Proteins c-myc; D000096:Acetone; D005557:Formaldehyde; C003043:paraform