title: Rat brain endoplasmic reticulum calcium pools are anatomically and functionally segregated.
abstract: Autoradiographic imaging can localize 45Ca2+ selectively accumulated via the Ca2+, Mg2(+)-ATPase into endoplasmic reticulum stores in rat brain slices. 45Ca2+ accumulation is markedly stimulated by oxalate and displays a heterogeneous distribution which resembles the mRNA distribution for a sarcoendoplasmic reticulum Ca2+, Mg2(+)-ATPase. Inositol 1,4,5-triphosphate [I(1,4,5)P3] inhibits 45Ca2+ accumulation selectively into regions corresponding to those enriched in I(1,4,5)P3 receptor-binding sites and in Ca2+, -Mg2(+)-ATPase mRNA. Thus rat brain endoplasmic reticulum calcium stores are anatomically and functionally differentiated.
authors.affiliation: Johns Hopkins University School of Public Health, Department of Toxicology, Baltimore, Maryland 21205.
authors.forename: A
authors.initials: A
authors.lastname: Verma
authors.forename: C A
authors.initials: CA
authors.lastname: Ross
authors.forename: D
authors.initials: D
authors.lastname: Verma
authors.forename: S
authors.initials: S
authors.lastname: Supattapone
authors.forename: S H
authors.initials: SH
authors.lastname: Snyder
mesh_terms: D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D017301:Ca(2+) Mg(2+)-ATPase / Q000235:genetics; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels; D002132:Calcium Radioisotopes; D000252:Calcium-Transporting ATPases / Q000235:genetics; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism / Q000494:pharmacology; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008297:Male; D010070:Oxalates / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear
chemical_list: D015220:Calcium Channels; D002132:Calcium Radioisotopes; D053496:Inositol 1,4,5-Trisphosphate Receptors; D010070:Oxalates; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D015544:Inositol 1,4,5-Trisphosphate; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D002118:Calcium