[Rapid diagnosis of cytomegalovirus hepatitis after liver transplantation. Use of monoclonal antibody E13 directed against an early viral antigen].
Liver biopsies obtained from 10 liver transplant patients for whom a cytomegalovirus (CMV) hepatitis was suspected on the basis of clinical and biological abnormalities have been studied by an immunohistological method using the monoclonal antibody E-13 directed against an early viral antigen. Biopsies were performed between the 30th and the 77th day after transplantation. In 7 of the 10 cases, CMV hepatitis was diagnosed by histological examination because of the association of lobular cytolysis, inflammatory infiltrate with neutrophils and intranuclear inclusions. Ten cases were positive with the E-13 antibody including the 3 cases in which histological examination was not conclusive. In all cases, positive results gave a nuclear staining pattern in hepatocytes (63%) or endothelial cells (40%), independently of the presence of inclusions. Rapid diagnosis of CMV hepatitis is of significant importance since anti-viral drugs, such as DHPG (Ganciclovir), are now efficient. The immunohistochemical method with E-13 could permit easy and rapid detection of CMV in liver specimens.
Spatz
A
A
Laboratoire Central d'Anatomie Pathologique, Hôpital Paul Brousse, Villejuif.
Tricottet
V
V
Saliba
F
F
Samuel
D
D
Bismuth
H
H
Reynès
M
M
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D001706:Biopsy; D002648:Child; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000473:pathology; D006525:Hepatitis, Viral, Human / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D017874:Immediate-Early Proteins; D007150:Immunohistochemistry; D016031:Liver Transplantation / Q000009:adverse effects* / Q000473:pathology; D008875:Middle Aged
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D017874:Immediate-Early Proteins; C044582:immediate-early proteins, cytomegalovirus
Diagnostic rapide des hépatites à cytomégalovirus après transplantation hépatique. Utilisation de l'anticorps monoclonal E13 dirigé contre un antigène viral précoce.