Localization of mRNA for testis-specific histone H1t by in situ hybridization.
H1t is a testis-specific H1 histone variant that appears in germ cells during the meiotic prophase of mammalian spermatogenesis. Using a tritiated antisense RNA probe, H1t mRNA was identified by in situ hybridization in the mid and late pachytene spermatocytes found in seminiferous tubules of approximately stages VII to XIII.
Kremer
E J
EJ
Department of Chemistry, University of South Carolina, Columbia 29208.
Kistler
W S
WS
D000818:Animals; D016375:Antisense Elements (Genetics); D003001:Cloning, Molecular; D006657:Histones / Q000235:genetics*; D008297:Male; D008540:Meiosis; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping; D013090:Spermatocytes / Q000166:cytology / Q000502:physiology; D013091:Spermatogenesis; D013737:Testis / Q000166:cytology / Q000502:physiology*
D016375:Antisense Elements (Genetics); D006657:Histones; D015347:RNA Probes; D012333:RNA, Messenger