Universality of c-fos transcriptional regulation: the Dyad Symmetry Element mediates activation by PMA in T lymphocytes.
We here have delineated the regulatory sequences responsible for c-fos transcriptional activation in human primary T lymphoblasts and in a human tumor T cell line (Jurkat), using transient transfection assays. Our results indicate that, as it has been demonstrated for fibroblastic or epithelial cells, the Dyad Symmetry Element is necessary and sufficient to confer responsiveness to an heterologous promoter in both cell types. Protein binding to this element was constitutive, as assessed by gel shift assays. These results suggest that c-fos transcriptional regulation occurs through a widely conserved mechanism in highly differentiated tissues.
Robin
P
P
Laboratoire d'Immunologie, CNRS URA 1156, Institut Gustave Roussy, Villejuif, France.
Trouche
D
D
Sassone-Corsi
P
P
Harel-Bellan
A
A
D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D016762:Genes, fos; D006801:Humans; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic; D014162:Transfection
D004268:DNA-Binding Proteins; D009838:Oligodeoxyribonucleotides; D013755:Tetradecanoylphorbol Acetate