title: A rapid method for the quantitation of estrogen receptors in small amounts of tissue.
abstract: A rapid and highly reproducible protocol which permits the detection of quantities of estrogen receptor as low as 5 fmol/mg protein is described. The separation of free and receptor-bound hormone is achieved by specific immunoprecipitation of the hormone-receptor complex. This procedure can be performed without perturbing the equilibrium of the binding reaction.
authors.affiliation: Milano Molecular Pharmacology Lab, University of Milan, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Bettini
authors.forename: A
authors.initials: A
authors.lastname: Maggi
mesh_terms: D000818:Animals; D003600:Cytosol / Q000737:chemistry; D005260:Female; D051379:Mice; D051381:Rats; D011919:Rats, Inbred Strains; D011960:Receptors, Estrogen / Q000032:analysis* / Q000276:immunology; D015203:Reproducibility of Results
chemical_list: D011960:Receptors, Estrogen