title: Karyotypes and X chromosome inactivation in segregants of a murine X-autosome translocation, T(X;4)37H.
abstract: Karyotypes and X chromosome inactivation were studied in embryos obtained from female mice carrying T(X;4)37H translocation on day 6 to 8 of gestation by a BrdU-acridine orange method. A total of 18 different karyotypes were found in 477 embryos examined: 90.0% embryos were products expected from 2:2 alternate or adjacent 1 disjunction. 3:1 and adjacent 2 disjunctions accounted for approximately 8.0% and 0.7% conceptuses, respectively. In the embryo proper of balanced T37H/ + conceptuses, inactivation was random with respect to the normal X and the larger translocation X (4x) chromosome. In all the cells with the 4x inactive, the late replication apparently did not spread to the attached autosomal portion, although black/brown coat variegation implies spreading of inactivation into the autosomal region. The X chromosome segment deprived of the inactivation center remained active in all the cells examined and it exerted deleterious effects on embryonic or fetal development. Observation in embryos having two maternally derived X chromosomes showed that they were indeed resistant to inactivation in early extraembryonic cell lineages, and two copies of active X chromosomes in the trophectoderm fatally affected embryonic development due to inability to form the extraembryonic ectoderm and ectoplacental cone from the polar trophectoderm. In unbalanced X aneuploids the X chromosomes with the deletion were preferentially inactivated due to strong selection against nullisomy X.
authors.affiliation: Research Center for Molecular Genetics, Hokkaido University, Sapporo, Japan.
authors.forename: C S
authors.initials: CS
authors.lastname: Shao
authors.forename: N
authors.initials: N
authors.lastname: Takagi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003433:Crosses, Genetic; D004303:Dosage Compensation, Genetic; D005314:Embryonic and Fetal Development / Q000235:genetics; D005260:Female; D006579:Heterozygote; D007621:Karyotyping; D008297:Male; D051379:Mice; D014178:Translocation, Genetic