Drug-induced cell cycle perturbation in chemotherapy of patients with non-small cell lung cancer.
To observe in vivo cell cycle perturbation in the chemotherapy of lung cancer, tumour cell kinetics during the first course of chemotherapy were measured in seven patients with histologically-verified non-small cell lung cancer. The tumour cells were aspirated from six lymph nodes and one subcutaneous nodule both prior to treatment and twice weekly after the administration of chemotherapeutic agents. The nuclear DNA content of aspirated tumour cells was measured with a scanning microdensitometer at a wavelength of 550 nm after the modified Feulgen reaction. The cell population in cell cycle was estimated with a cumulated percentage scale. Marked cell cycle perturbation occurred within one week after initiation of chemotherapy. There was a decrease in the G1 cell population, from 70.6 +/- 9.1% to 26.1 +/- 11.4%, and a corresponding increase of cells in G2-M phase, from 21.4 +/- 8.7% to 63.7 +/- 10.0%. The proportion of cells in S phase was slightly increased from 8.0 +/- 1.5% to 10.1 +/- 3.2% during this period. The degree of cell cycle changes was unrelated to the clinical response to chemotherapy.
Kuo
S H
SH
Department of Clinical Pathology, College of Medicine, National Taiwan University, Taipei, Republic of China.
Luh
K T
KT
D000230:Adenocarcinoma / Q000737:chemistry / Q000188:drug therapy / Q000473:pathology*; D000328:Adult; D000368:Aged; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology* / Q000627:therapeutic use; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry / Q000188:drug therapy / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects*; D002945:Cisplatin / Q000008:administration & dosage / Q000494:pharmacology; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000494:pharmacology; D004273:DNA, Neoplasm / Q000032:analysis; D003720:Densitometry; D004317:Doxorubicin / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D006801:Humans; D007399:Interphase / Q000187:drug effects; D008175:Lung Neoplasms / Q000737:chemistry / Q000188:drug therapy / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011003:Ploidies
D004273:DNA, Neoplasm; D004317:Doxorubicin; D003520:Cyclophosphamide; D002945:Cisplatin