Detection of free radical scavenging activity of schisanhenol by electron spin resonance.
It was reported that Schisanhenol (Sal) isolated from Schisandrae Rubriflora inhibited lipid peroxidation induced by ferrous-cysteine and NADPH-ascorbic acid. In this studies the oxygen radical scavenging activity of Sal was detected by electron spin resonance (ESR) and spin trapping. Sal was shown to scavenge oxygen radicals produced by human neutrophils (Neu) stimulated by tetradecanoylphorbol acetate (TDPA). But no effect of Sal was seen on oxygen consumption measured by spin label oximetry in Neu during respiratory burst. In Fenton reaction system, the inhibitory rate of hydroxyl radical by Sal was 34.4%. In xanthine-xanthine oxidase and uv-irradiation of riboflavin systems, Sal scavenged superoxide anion radical by 26.1% and 21.9%, respectively. In all these systems the action of Sal was more potent than that of vitamin E. It may be concluded that Sal possesses a free radical-scavenging activity.
Lin
T J
TJ
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Liu
G T
GT
Li
X J
XJ
Zhao
B L
BL
Xin
W J
WJ
D034242:Cyclooctanes; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers; D005609:Free Radicals; D006801:Humans; D017705:Lignans; D009504:Neutrophils / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D011083:Polycyclic Compounds / Q000494:pharmacology*
D034242:Cyclooctanes; D016166:Free Radical Scavengers; D005609:Free Radicals; D017705:Lignans; D011083:Polycyclic Compounds; C034734:schizandrin A; C045309:schisanhenol; D010100:Oxygen