IgE-mediated inhalant allergy against human corticotropin-releasing hormone.
A 25-year-old female developed IgE-mediated sensitization against human recombinant corticotropin-releasing hormone (CRH) with symptoms of allergic rhinoconjunctivitis and bronchial asthma. The occupational allergy was proved by positive skin prick test, bronchial provocation, dose-dependent histamine release, RAST measurements with CRH allergen (RAST class 3) and RAST inhibition. Using the immunoblot technique, a single allergen band with a molecular weight of less than 14.4 kD in the range between the isoelectric point 5.2 and 5.7 was detected for the CRH extract. Since no endocrinological and behavioral disorders were found, increased CRH-specific IgE was not able to influence the regulatory control of this neuropeptide. After 18 months of avoiding the occupational CRH exposure allergen-specific histamine release and RAST were negative.
Kleine-Tebbe
J
J
Department of Clinical Immunology and Asthma OPD, Rudolf Virchow University Hospital, Free University of Berlin, FRG.
Wahl
R
R
Dierks
K
K
Kunkel
G
G
D000328:Adult; D001249:Asthma / Q000139:chemically induced; D001491:Basophils / Q000187:drug effects / Q000378:metabolism; D015153:Blotting, Western; D001985:Bronchial Provocation Tests; D003346:Corticotropin-Releasing Hormone / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D004342:Drug Hypersensitivity / Q000175:diagnosis / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006632:Histamine / Q000096:biosynthesis; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D007526:Isoelectric Point; D007962:Leukocytes / Q000378:metabolism; D016273:Occupational Exposure; D011852:Radioallergosorbent Test; D011994:Recombinant Proteins / Q000276:immunology; D012044:Regression Analysis; D012221:Rhinitis, Allergic, Perennial / Q000139:chemically induced; D012882:Skin Tests
D011994:Recombinant Proteins; D007073:Immunoglobulin E; D006632:Histamine; D003346:Corticotropin-Releasing Hormone