title: Comparative studies on proteasomes (multicatalytic proteinases) isolated from spermatozoa and eggs of sea urchins.
abstract: 1. Proteasomes (multicatalytic proteinases) have been purified from spermatozoa and eggs of sea urchins by successive chromatographies on DEAE-cellulose, hydroxylapatite, and Sepharose 6B. 2. The isolated sperm and egg proteasomes showed similar properties such as molecular weight (both 630,000) and multiple catalytic activity (pH optimum, substrate specificity, inhibitor-susceptibility and SDS-stimulation). 3. Distinct protein components were detected between sperm and egg proteasomes by SDS-polyacrylamide gel electrophoresis and immunoblot analysis using antibody against egg proteasome.
authors.affiliation: Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Saitoh
authors.forename: H
authors.initials: H
authors.lastname: Kawahara
authors.forename: H
authors.initials: H
authors.lastname: Miyamatsu
authors.forename: H
authors.initials: H
authors.lastname: Yokosawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002384:Catalysis; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000302:isolation & purification; D015151:Immunoblotting; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000302:isolation & purification; D010063:Ovum / Q000201:enzymology*; D046988:Proteasome Endopeptidase Complex; D012617:Sea Urchins / Q000737:chemistry; D013094:Spermatozoa / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D009097:Multienzyme Complexes; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex