title: Enrichment of oncolytic cells for adoptive therapy using centrifugal elutriation.
abstract: We have shown that centrifugal elutriation (CE) can be used to obtain large numbers of peripheral blood lymphocytes enriched for natural killer (NK) cell activity. Lymphocytes distributed in fraction 3 (F3) were enriched 2.3 fold for large granular lymphocytes, LGL, and 1.8 fold for cells with CD16+ phenotype. Both unseparated and F3 cells proliferated and were activated following culture with interleukin-2 (IL-2), although the NK-enriched cells displayed superior cytolytic activity against tumor cell lines, as well as against fresh leukemic blasts. Comparison of the cell surface phenotype of long-term cultures indicated a significantly higher frequency of CD56+, CD3- and CD16+ NK cells, and a reduced content of CD3+ T cells in F3 populations. These results suggest that IL-2 activated NK-enriched CE-fractionated lymphocytes may be more effective than unseparated mononuclear cells for adoptive therapy purposes.
authors.affiliation: Department of General Surgery, M.D. Anderson Cancer Center, Houston, TX 77030.
authors.forename: C A
authors.initials: CA
authors.lastname: Savary
authors.forename: E
authors.initials: E
authors.lastname: Lotzová
authors.forename: B
authors.initials: B
authors.lastname: Lichtiger
authors.forename: E J
authors.initials: EJ
authors.lastname: Freireich
mesh_terms: D002498:Centrifugation; D003601:Cytotoxicity Tests, Immunologic; D006098:Granulocytes / Q000187:drug effects / Q000276:immunology; D006801:Humans; D016219:Immunotherapy, Adoptive; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*; D007937:Leukapheresis / Q000379:methods*; D007938:Leukemia / Q000276:immunology*; D008213:Lymphocyte Activation; D014407:Tumor Cells, Cultured
chemical_list: D007376:Interleukin-2