title: Description of two anaerobic fungal strains from the bovine rumen and influence of diet on the fungal population in vivo.
abstract: Neocallimastix sp. NC71 and Piromyces sp. PC12 isolated from the calf remen grew optimally at 39 degrees C and pH 6.5-6.7, utilized a wide range of mono-, oligo- and polysaccharides and exhibited CMCase, Avicelase, cellobiase, amylase and xylanase activities. The end-products of wheat straw fermentation by both strains were acetate, formate, ethanol and lactate. The number of Neocallimastix sp. zoospores in the rumen of cows in the first 3 h after feeding with hay-silage-concentrate diets varied from 7 x 10(3) to 5.4 x 10(5) ml-1; the number of uniflagellate zoospores varied from 10(4) to 10(5) ml-1. Fungal zoosporgenesis and colonization of plant substrates in the rumen were induced by feed intake and were favoured by increased levels of crude fibre in the diet.
authors.affiliation: Laboratory of Microbial Physiology, Institute of Biochemistry and Physiology of Micro-organisms, USSR Academy of Sciences, Pushchino, Moscow Region.
authors.forename: V A
authors.initials: VA
authors.lastname: Kostyukovsky
authors.forename: O N
authors.initials: ON
authors.lastname: Okunev
authors.forename: B V
authors.initials: BV
authors.lastname: Tarakanov
mesh_terms: D000818:Animals; D002417:Cattle / Q000382:microbiology*; D002475:Cellobiose / Q000378:metabolism; D008411:Chytridiomycota / Q000166:cytology / Q000302:isolation & purification / Q000502:physiology*; D004032:Diet; D005285:Fermentation; D005658:Fungi / Q000166:cytology / Q000302:isolation & purification / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D010209:Paper; D012417:Rumen / Q000382:microbiology*; D012820:Silage; D013172:Spores, Fungal / Q000737:chemistry; D013696:Temperature
chemical_list: D002475:Cellobiose