Presence of a transposon-like element in the promoter region of an inactive patatin gene in Solanum tuberosum L.
The promoter of the PGT3 patatin gene belonging to the class II subfamily is highly homologous to other class II patatin genes except for a 736 bp insertion in front of the putative transcription start site. The insertion is characterized by structural features resembling a transposable element such as an 11 bp inverted repeat at the termini and an 8 bp duplication flanking the insertion site. Despite the high homology to active patatin genes, fusion of its promoter to the beta-glucuronidase reporter gene does not lead to detectable beta-glucuronidase (GUS) activity in transgenic potato or tobacco plants, suggesting that the inactivation of this gene might be caused by the insertion of the transposon like element.
Köster-Töpfer
M
M
IGF Berlin, Federal Republic of Germany.
Frommer
W B
WB
Rocha-Sosa
M
M
Willmitzer
L
L
D001483:Base Sequence; D015139:Blotting, Southern; D002265:Carboxylic Ester Hydrolases; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004251:DNA Transposable Elements; D017343:Genes, Plant; D008961:Models, Structural; D008969:Molecular Sequence Data; D005810:Multigene Family; D009690:Nucleic Acid Conformation; D010940:Plant Proteins / Q000235:genetics*; D010947:Plants, Toxic; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D011198:Solanum tuberosum / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D004251:DNA Transposable Elements; D010940:Plant Proteins; C043222:patatin protein, Solanum tuberosum; D004247:DNA; D002265:Carboxylic Ester Hydrolases