The isolation and partial characterization of a radiation-sensitive clone of a human bladder carcinoma cell line.
The isolation of radiosensitive mammalian cell mutants has been limited largely to rodent cells. We report here the isolation of a radiosensitive variant (S40b) from 3648 analyzed clones of a mutagenized human bladder carcinoma cell line (MGH-U1). The surviving fraction at 2 Gy was 0.32 for S40b cells compared with 0.72 for MGH-U1 cells. Split-dose recovery experiments done at several doses did not show a difference between S40b and the parental line at any dose. Irradiation at the low dose rate of 2 cGy min-1 did not show a decreased dose-rate sparing at isoeffect in S40b cells. There was no difference between MGH-U1 and S40b cells in the amount of DNA damage present immediately after irradiation, as detected by neutral filter elution. The S40b variant therefore represents a new tool for the examination of the processing of DNA damage in human cells.
McMillan
T J
TJ
Radiotherapy Research Unit, Institute of Cancer Research, Sutton, Surrey, United Kingdom.
Holmes
A
A
D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D002999:Clone Cells; D004249:DNA Damage / Q000528:radiation effects; D004273:DNA, Neoplasm / Q000528:radiation effects; D006801:Humans; D011829:Radiation Dosage; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured / Q000528:radiation effects; D001749:Urinary Bladder Neoplasms / Q000532:radiotherapy*
D004273:DNA, Neoplasm