Hydrolysis of chylomicron polyenoic fatty acid esters with lipoprotein lipase and hepatic lipase.
The lipolysis of rat chylomicron polyenoic fatty acid esters with bovine milk lipoprotein lipase and human hepatic lipase was examined in vitro. Chylomicrons obtained after feeding fish oil or soy bean oil emulsions were used as substrates. The lipolysis was followed by gas chromatography or by using chylomicrons containing radioactive fatty acids. Lipoprotein lipase hydrolyzed eicosapentaenoic (20:5) and arachidonic acid (20:4) esters at a slower rate than the C14-C18 acid esters. More 20:5 and 20:4 thus accumulated in remaining tri- and diacylglycerols. Eicosatrienoic, docosatrienoic and docosahexanoic acids exhibited an intermediate lipolysis pattern. When added together with lipoprotein lipase, hepatic lipase increased the rate of lipolysis of 20:5 and 20:4 esters of both tri- and diacylglycerols. Addition of NaCl (final concentration 1 M) during the course of lipolysis inhibited lipoprotein lipase as well as the enhancing effect of hepatic lipase on triacylglycerol lipolysis. Hepatic lipase however, hydrolyzed diacylglycerol that had already been formed. Chylomicron 20:4 and 20:5 esters thus exhibit a relative resistance to lipoprotein lipase. It is suggested that the tri- and diacylglycerol species containing these fatty acids may accumulate at the surface of the remnant particles and act as substrate for hepatic lipase during a concerted action of this enzyme and lipoprotein lipase.
Melin
T
T
Department of Medicine, University of Lund, Sweden.
Qi
C
C
Bengtsson-Olivecrona
G
G
Akesson
B
B
Nilsson
A
A
D000818:Animals; D002914:Chylomicrons / Q000032:analysis / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism*; D005395:Fish Oils / Q000378:metabolism; D005989:Glycerides / Q000032:analysis; D066298:In Vitro Techniques; D008049:Lipase / Q000494:pharmacology*; D008066:Lipolysis; D008071:Lipoprotein Lipase / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D002914:Chylomicrons; D005227:Fatty Acids; D005395:Fish Oils; D005989:Glycerides; D008049:Lipase; D008071:Lipoprotein Lipase