Effect of pH shifts on radiosensitivity of human lymphocytes irradiated in the G2 stage.
Experiments were carried out using human lymphocytes in order to test the effect of pH shifts on radiosensitivity of cells irradiated in the G2 stage. In our culture conditions constant variations in medium pH over the range of 7.4-6.8 were observed as a function of incubation time after PHA stimulation; in addition the pH of the medium was adjusted in parallel cultures over the range of 5.9-8.2. Then we exposed the cultures with different pH to a treatment of X-rays delivered 2 h before fixation. The pH of the medium was found to greatly affect the yield of induced chromatid aberrations.
Bosi
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Dipartimento di Genetica e Biologia Molecolare, Università di Roma, La Sapienza, Italy.
Micheli
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Pietrosanti
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Olivieri
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G
D002455:Cell Division; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D016195:G2 Phase; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects*; D010835:Phytohemagglutinins
D010835:Phytohemagglutinins