Immunohistochemistry of secretory transglutaminase from rodent prostate.
Transglutaminases are Ca2(+)-dependent intra- and extracellular enzymes catalyzing the cross-linking between proteins and/or polyamines, thereby eliciting divergent physiological effects such as fibrin clot stabilization or hair follicle cross-linking. A secretory transglutaminase (EC 2.3.2.13) was isolated from the coagulating gland of the rat. The protein is highly glycosylated. A fraction purified to homogeneity was used as an antigen to raise polyclonal antibodies in rabbits. These antibodies were used to identify the secretion sites of the protein within the male accessory sex glands as well as to study the immunological relationships of the respective antigen within different organs of different species. In the rat, the coagulating gland and likewise the dorsal prostate gave a positive immunoreaction. In the guinea pig, a closely related protein was detected in the anterior prostate. No cross-reactivity was found with membrane-bound transglutaminase from liver, erythrocytes or blood clotting factor XIIIa. The intraluminal secretion of the aforementioned glands was only weakly stained. No secretory granules were observed in the glandular epithelium but instead bleb-like structures reminiscent of apocrine secretion. A slight background stain of the epithelium remained even in castrated animals where secretion is largely suppressed. The background stain is attributed to a tissue-type, membrane-bound, non-secretory transglutaminase that is not androgen dependent, but instead synthesized only after androgen deprivation.
Seitz
J
J
Department of Anatomy and Cell Biology, Marburg/Lahn, Federal Republic of Germany.
Keppler
C
C
Rausch
U
U
Aumüller
G
G
D000728:Androgens / Q000502:physiology; D000818:Animals; D000918:Antibody Specificity; D005455:Fluorescent Antibody Technique; D005837:Genitalia, Male / Q000201:enzymology; D008297:Male; D011467:Prostate / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D012726:Sexual Behavior, Animal / Q000502:physiology; D011503:Transglutaminases / Q000378:metabolism*
D000728:Androgens; D011503:Transglutaminases