Stimulation of human T cell colony growth by a lymphocyte colony enhancement factor derived from lymphocyte subpopulations.
Blood mononuclear cells (MNC) develop into T cell colonies when the cells are sensitized with PHA and seeded in a two-layer soft agar system. Conditioned medium (CM) derived from MNC enhanced lymphocyte colony formation when it was added to the culture system. CFU-TL appear to be stimulated into colony formation by molecules secreted by lymphocyte subpopulations contained in the seeded cells. In this study, human peripheral blood MNC were fractionated by a battery of techniques into adherent, E+, CD4+, CD8+, B and null cells. CM was prepared from each of the subpopulations and its effects on T cell colony growth assayed. All the lymphocyte subpopulations were found to generate lymphocyte colony enhancement factor (LCEF). After several purification procedures, CM prepared from CD4 and CD8+, displayed LCEF activity corresponding to proteins of molecular weight 30-40 and 100-140 kD.
Lenz
R
R
Department of Life Sciences, Bar Ilan University, Ramat Gan, Israel.
Alter
A
A
Radnay
J
J
Goldman
J
J
Cooper
O
O
Sredni
D
D
Rozenszajn
L A
LA
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D001402:B-Lymphocytes / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D016827:CD8 Antigens; D003115:Colony-Stimulating Factors / Q000096:biosynthesis* / Q000302:isolation & purification / Q000502:physiology; D003470:Culture Media; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000378:metabolism*; D008215:Lymphocytes, Null / Q000378:metabolism; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000166:cytology*
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D003115:Colony-Stimulating Factors; D003470:Culture Media