title: Expression of clumping and fibrinogen-binding activities of Staphylococcus aureus at various growth stages.
abstract: Clumping and fibrinogen-binding activities of 4 Staphylococcus aureus strains (Cowan I, Newman D2C, Wood 46 and NCTC 5655) were assayed with a semiquantitative clumping test and an enzyme-linked immunosorbent assay (ELISA), respectively. Distinct positive clumping was detected with whole cells of the 3 strains except Wood 46. Amounts of fibrinogen required for a definite clumping depended greatly on strains as well as on their growth phases. On the other hand, fibrinogen-binding activities were detected both in culture supernatants and in cell lysates of all the 4 strains, and the levels were rather comparable with one another and relatively steady through their growth cycles. No significant correlation was thus found among expression behavior of clumping and fibrinogen-binding activities.
authors.affiliation: Department of Bacteriology, Nara Medical College.
authors.forename: K
authors.initials: K
authors.lastname: Yonemasu
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: R
authors.initials: R
authors.lastname: Ohmae
authors.forename: S
authors.initials: S
authors.lastname: Kashiba
mesh_terms: D002352:Carrier Proteins / Q000502:physiology*; D002449:Cell Aggregation / Q000502:physiology*; D003030:Coagulase / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005340:Fibrinogen / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000254:growth & development / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D003030:Coagulase; D005340:Fibrinogen