Expression of clumping and fibrinogen-binding activities of Staphylococcus aureus at various growth stages.
Clumping and fibrinogen-binding activities of 4 Staphylococcus aureus strains (Cowan I, Newman D2C, Wood 46 and NCTC 5655) were assayed with a semiquantitative clumping test and an enzyme-linked immunosorbent assay (ELISA), respectively. Distinct positive clumping was detected with whole cells of the 3 strains except Wood 46. Amounts of fibrinogen required for a definite clumping depended greatly on strains as well as on their growth phases. On the other hand, fibrinogen-binding activities were detected both in culture supernatants and in cell lysates of all the 4 strains, and the levels were rather comparable with one another and relatively steady through their growth cycles. No significant correlation was thus found among expression behavior of clumping and fibrinogen-binding activities.
Yonemasu
K
K
Department of Bacteriology, Nara Medical College.
Sasaki
T
T
Ohmae
R
R
Kashiba
S
S
D002352:Carrier Proteins / Q000502:physiology*; D002449:Cell Aggregation / Q000502:physiology*; D003030:Coagulase / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005340:Fibrinogen / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000254:growth & development / Q000378:metabolism
D002352:Carrier Proteins; D003030:Coagulase; D005340:Fibrinogen