Inhibitory effects of 3-imino-5-phenyl-3H-1,2-dithiole on poliovirus type 1 replication in vitro.
The effect of 3-imino-5-phenyl-3H-1,2-dithiole (PDTI) on different steps of the replicative cycle of poliovirus type 1 in HEp-2 cells was studied. This compound inhibited the replication of poliovirus type 1 as shown by cytopathic effect and virus yield reduction. This inhibitory action was not due to a virucidal effect, nor did the cells to have been pretreated. Under one-step growth conditions 3-imino-5-phenyl-3H-1,2-dithiole caused the greatest inhibition if added within 1 h after poliovirus adsorption. [5-3H]uridine incorporation into RNA showed that PDTI reduced poliovirus RNA synthesis. In fact, in the presence of PDTI viral RNA synthesis was shut off completely at 2 h post infection, and at 4 h post infection viral RNA synthesis was reduced by 50%. The compound may have an inhibitory effect on the early transcriptional and/or replicative functions of the poliovirus genome.
Garozzo
A
A
Institute of Microbiology, University of Catania, Italy.
Pinizzotto
M R
MR
Furneri
P M
PM
Baratta
D
D
Guerrera
F
F
Castro
A
A
Tornetta
B
B
Geremia
E
E
D000998:Antiviral Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007097:Imines / Q000494:pharmacology*; D011051:Poliomyelitis / Q000188:drug therapy; D017955:Poliovirus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D012367:RNA, Viral / Q000096:biosynthesis; D013936:Thymidine / Q000235:genetics; D014529:Uridine / Q000235:genetics; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D004279:DNA, Viral; D007097:Imines; D012367:RNA, Viral; C068484:3-imino-5-phenyl-3H-1,2-dithiole; D013936:Thymidine; D014529:Uridine