title: Development of bacillus Calmette-Guérin-induced antitumor activity in peripheral blood mononuclear cells.
abstract: Peripheral blood mononuclear cells (PBMNCs) after bladder instillation of bacillus Calmette-Guérin (BCG) to patients with superficial bladder carcinoma showed lytic activity against natural killer (NK)-sensitive and NK-resistant target tumor cells. PBMNCs in normal volunteers were isolated and cultured with BCG (5 micrograms/ml) in vitro. Maximum cytotoxicity was obtained within 24 h. Cytosine arabinoside as a DNA synthesis inhibitor did not inhibit this killer cell activation. Monoclonal antibody treatment revealed that both precursor and effector cells were NK cell type. However, effector cells had a wider spectrum of target cells than usual NK cells. The recognition specificity from cold target competition experiments was selective.
authors.affiliation: Department of Urology, Nagasaki University School of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Koga
authors.forename: K
authors.initials: K
authors.lastname: Taniguchi
authors.forename: M
authors.initials: M
authors.lastname: Nishikido
authors.forename: S
authors.initials: S
authors.lastname: Kubota
authors.forename: S
authors.initials: S
authors.lastname: Yamashita
authors.forename: H
authors.initials: H
authors.lastname: Kanetake
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
mesh_terms: D000283:Administration, Intravesical; D001500:BCG Vaccine / Q000276:immunology / Q000627:therapeutic use*; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D001749:Urinary Bladder Neoplasms / Q000276:immunology / Q000628:therapy*
chemical_list: D001500:BCG Vaccine