title: Evidence for phosphatidylinositol anchorage of opioid binding proteins in rat brain.
abstract: Treatment of rat brain sections and of thalamic and striatal membranes with phosphatidylinositol phospholipase C (PIPLC), an enzyme known to hydrolyse only phosphatidylinositol and its derivatives, significantly alters the specific binding of mu- and delta-opioid ligands on their receptors. These results suggest that some opioid binding proteins are membrane-anchored by a glycosylphosphatidylinositol (GPI) linkage.
authors.affiliation: INSERM U.114, Chaire de Neuropharmacologie, Collège de France, Paris.
authors.forename: F
authors.initials: F
authors.lastname: Trovero
authors.forename: J
authors.initials: J
authors.lastname: Glowinski
authors.forename: M
authors.initials: M
authors.lastname: Lévy
mesh_terms: D000818:Animals; D001345:Autoradiography; D001923:Brain Chemistry / Q000502:physiology*; D003342:Corpus Striatum / Q000378:metabolism; D020881:Enkephalin, D-Penicillamine (2,5)-; D004745:Enkephalins / Q000378:metabolism; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008566:Membranes / Q000378:metabolism; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases; D011485:Protein Binding / Q000502:physiology; D051381:Rats; D011957:Receptors, Opioid / Q000378:metabolism*; D013788:Thalamus / Q000378:metabolism; D001615:beta-Endorphin / Q000378:metabolism
chemical_list: D004745:Enkephalins; D007457:Iodine Radioisotopes; D010716:Phosphatidylinositols; D011957:Receptors, Opioid; D001615:beta-Endorphin; D020881:Enkephalin, D-Penicillamine (2,5)-; D010727:Phosphoric Diester Hydrolases; D043265:Phosphatidylinositol Diacylglycerol-Lyase