title: Sequencing PCR DNA amplified directly from a bacterial colony.
abstract: We show that PCR product asymmetrically amplified directly from a bacterial colony can be sequenced to yield results as good as those obtained when purified template DNA is used for the PCR amplification step. With either template, greater than 300 nucleotides can be read from a typical sequencing reaction. Taq DNA polymerase was used for both the PCR amplification and sequencing reactions.
authors.affiliation: Department of Microbiology, University of Tennessee, Knoxville 37996-0845.
authors.forename: M A
authors.initials: MA
authors.lastname: Hofmann
authors.forename: D A
authors.initials: DA
authors.lastname: Brian
mesh_terms: D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D003332:Coronaviridae / Q000235:genetics; D004247:DNA; D003854:Deoxyribonucleotides; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D003854:Deoxyribonucleotides; D004247:DNA