Neurobiology of human oligodendrocytes in culture.
Enriched populations of oligodendrocytes were isolated from adult human brains of 3-15 hours postmortem using the trypsinin digestion-Percoll density gradient method and were cultured for an extended period of time up to 6 months. Cell type specific antigens that were expressed by oligodendrocytes were galactocerebroside, myelin basic protein, proteolipid protein, 2'3'-cyclic nucleotide 3'-phosphohydrolase and myelin-associated glycoprotein. In addition, HLA-A,B,C and HLA-DR, respectively, Class I and Class II antigens of the major histocompatibility complex, were demonstrated on oligodendrocytes. Three classes of gangliosides, GM1, GM4, and GD3, were also demonstrated on oligodendrocytes, while GM1 and GM4 gangliosides were detected on the surface of astrocytes. The presence of "transitional" or "bipotential" glial cells that were derived from oligodendrocytes and that expressed both oligodendroglial and astrocytic phenotypes was demonstrated. Treatment of the cells by cyclic AMP and its derivatives reversed this dual phenotypic expression back to the oligodendroglial trait. Electron microscopic examination of oligodendrocytes indicated that they were capable of synthesizing and assembling myelin sheaths in culture in the absence of any neuronal signal input.
Kim
S U
SU
Division of Neurology, University of British Columbia, Vancouver, Canada.
D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D005699:Galactosylceramides; D005732:Gangliosides / Q000494:pharmacology; D005904:Glial Fibrillary Acidic Protein; D006680:HLA Antigens / Q000032:analysis; D015490:HTLV-I Infections / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D008285:Major Histocompatibility Complex / Q000276:immunology; D008854:Microscopy, Electron; D008875:Middle Aged; D009836:Oligodendroglia / Q000502:physiology*
D005699:Galactosylceramides; D005732:Gangliosides; D005904:Glial Fibrillary Acidic Protein; D006680:HLA Antigens; C002454:galactocerebroside; D000242:Cyclic AMP