title: The 987P gene cluster in enterotoxigenic Escherichia coli contains an STpa transposon that activates 987P expression.
abstract: The genetic determinant for the production of 987P fimbriae has been cloned into pBR322. Analysis of frequently occurring deletions in the resultant recombinant plasmid, pPK180, revealed that the 987P gene cluster contains a transposon that encodes the synthesis of heat-stable enterotoxin STpa and is flanked by inverted repeats of IS1. Hybridization experiments with STpa- and 987P-specific probes demonstrated that a variety of STpa+ 987P+ wild-type Escherichia coli strains contained contiguous STpa-987P DNA, most likely on their chromosome. Transcription of the 987P gene cluster appeared to be activated by the adjacent IS1 element.
authors.affiliation: Department of Molecular Microbiology, Faculty of Biology, Free University, Amsterdam, The Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: Klaasen
authors.forename: M J
authors.initials: MJ
authors.lastname: Woodward
authors.forename: F G
authors.initials: FG
authors.lastname: van Zijderveld
authors.forename: F K
authors.initials: FK
authors.lastname: de Graaf
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics*; D010861:Fimbriae, Bacterial; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial