Detection of px gene product of bovine leukemia virus in infected cells.
Bovine leukemia virus (BLV), like its closest relatives human T-cell leukemia virus-I and II, contain a 'px' gene, between the 'env' gene and the 3' long terminal repeat in its genome. A monoclonal antibody prepared against a synthetic oligopeptide whose sequence was deduced from highly conserved region of 'px' gene of BLV, was used to detect the presence of 'px' gene product in chronically BLV infected synchronised cells. By immunoperoxidase staining the 'px' gene product was detected maximum after 6-9 hr after synchronization in the nucleus of the cells which demonstrated the close interaction of it with viral DNA which is integrated with host cell genome.
Grover
Y P
YP
INSERM U-117, Unite de Recherches de Radiobiologie, Experimentale et de Cancerologie, Fondation Bergonie, Bordeaux, France.
Guillemain
B
B
Mamoun
R
R
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D016356:Gene Products, tax / Q000032:analysis* / Q000235:genetics; D016355:Genes, pX; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D008969:Molecular Sequence Data; D015682:Retroviridae Proteins, Oncogenic / Q000032:analysis* / Q000235:genetics; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics
D016356:Gene Products, tax; D015682:Retroviridae Proteins, Oncogenic; D014764:Viral Proteins; C059786:transactivator protein p38(tax)