Functional analysis of clonally expanded CD8, TCR gamma delta T cells in a patient with chronic T-gamma lymphoproliferative disease.
Leukemic cells from a patient with T-gamma lymphocytosis were found to have the surface phenotype, CD3+, CD4-, CD8+, Leu19+, TCR delta 1+, WT31-. The clonal nature of the TCR gamma delta T cell proliferation was documented by flow cytometry and Southern blot analysis. Morphologically, they were large to medium-sized mature lymphocytes with cytoplasmic granules. Functionally, the cells revealed strong cytotoxic activities against NK-sensitive target cells, but had neither killer T cell activity nor suppressive activity on PWM-driven immunoglobulin synthesis by B cells. Interestingly, both suppressive and cytotoxic T cell activities were recovered with the depletion of CD8+ T cells. These studies may suggest some functions of the CD8+ population of human TCR gamma delta T cells in a normal immune system.
Morikawa
K
K
Department of Internal Medicine, Shimane Medical University, Izumo, Japan.
Oseko
F
F
Hara
J
J
Kobayashi
S
S
Nakano
A
A
Morikawa
S
S
D000368:Aged; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis / Q000502:physiology*; D014408:Biomarkers, Tumor / Q000032:analysis; D015139:Blotting, Southern; D018801:CD2 Antigens; D017252:CD3 Complex; D016827:CD8 Antigens; D002455:Cell Division / Q000187:drug effects; D003602:Cytotoxicity, Immunologic; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007376:Interleukin-2 / Q000378:metabolism; D037102:Lectins / Q000494:pharmacology; D008218:Lymphocytosis / Q000276:immunology; D008232:Lymphoproliferative Disorders / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis / Q000502:physiology*; D011971:Receptors, Immunologic / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D014408:Biomarkers, Tumor; D018801:CD2 Antigens; D017252:CD3 Complex; D016827:CD8 Antigens; D007136:Immunoglobulins; D007376:Interleukin-2; D037102:Lectins; D011948:Receptors, Antigen, T-Cell; D011971:Receptors, Immunologic