Involvement of alpha- and beta-PKC in the differentiation of 3T3-L1 cells.
Present investigation revealed that 3T3-L1 cells contained two protein kinase C (PKC) subspecies, i.e. alpha- and beta-PKC. These cells treated by staurosporine, a specific inhibitor of PKC, differentiated to adipocytes more remarkably than those without its treatment. Their alpha- and beta-PKC activity decreased to 49% and 18% of the staurosporine-untreated cells respectively, and GPDH activity, a marker enzyme of adipocytes, increased to 143%. Thus, both alpha- and beta-PKC seemed to be associated with the adipose conversion in 3T3-L1 cells.
Ueda
H
H
Second Department of Internal Medicine, Kobe University School of Medicine, Japan.
Ishida
Y
Y
Taniguchi
H
H
D016475:3T3 Cells / Q000166:cytology / Q000187:drug effects; D000470:Alkaloids / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation; D002853:Chromatography, Liquid; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D019311:Staurosporine
D000470:Alkaloids; D005993:Glycerolphosphate Dehydrogenase; D011493:Protein Kinase C; D019311:Staurosporine