[Chemical mapping of normal and tumorous gastric mucosa by analytical ion microscopy].
The analytical ion microscopy (AIM) makes possible quantitative mapping of chemical elements on tissue section this method was applied to the study of chlorine (Cl-) and sulfur (S-) distributions on chemically fixed stomach sections obtained by biopsy. The Cl- and S- images were correlated with the histological structure of the tissue obtained by the phosphorus distribution on the same field or by the photonic image of a serial section. In normal tissue, Cl- and S- were evidenced in cytoplasm with accumulation at the apical cell border while in gastric adenocarcinoma both elements were distributed without precise topography. The use of cryo-prepared specimens should offer the possibility to study the changes of chlorine and sulfur content in functional pathology of stomach.
Papadopoulos
S
S
Laboratoire de Microscopie Ionique INSERM U66, Institut Gustave-Roussy, Villejuif.
Fragu
P
P
Fabri
M C
MC
Rougier
P
P
Prade
M
M
D005753:Gastric Mucosa / Q000737:chemistry*; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D008853:Microscopy / Q000379:methods; D012016:Reference Values; D013048:Specimen Handling / Q000379:methods; D013274:Stomach Neoplasms / Q000737:chemistry*
Imagerie chimique de la muqueuse gastrique normale et tumorale, obtenue par microscopie ionique analytique.