title: Actions of acetylcholine and GABA on spontaneous contractions of the filariid, Dipetalonema viteae.
abstract: 1. Isotonic contractions were recorded from the filarial nematode, Dipetalonema viteae (Acanthocheilonema viteae), in an isolated tissue chamber. 2. Nicotine (10(-6) M) and pilocarpine (10(-5) M) increased the spontaneous contractions in the intact filariid, but acetylcholine (ACh, 10(-4) M) and muscarine (10(-5) M) were inactive. 3. When ACh was applied to an opened D. viteae, it was 10,000 times more potent. This indicates that the cuticle is an effective barrier to the penetration of ACh to the muscle cells. 4. The effects of ACh on the opened D. viteae were not affected by hexamethonium (10(-3) M) or atropine (10(-5) M) and were only partially reduced by (+)-tubocurarine (10(-4) M). 5. gamma-Aminobutyric acid (GABA, 10(-3) M) reduced the spontaneous activity of the intact D. viteae; however, the effect of GABA had a slow onset and recovery. Muscimol (10(-5) M) was more potent than GABA and had a more rapid onset and recovery. 6. GABA was 1,000 times more potent on the opened D. viteae than on the intact D. viteae. Baclofen (10(-3) M) was inactive on both preparations. 7. The effect of GABA was not antagonized by bicuculline (10(-4) M), picrotoxin (10(-5) M or penicillin G (10(-3) M). 8. It is concluded that the filariid cuticle acts like a lipid structure and blocks the penetration of polar substances, such as ACh and GABA. Also, due to the lack of efficacy of the ACh and GABA antagonists, it was concluded that the nematode receptors are somewhat different from the mammalian ACh and GABA receptors.
authors.affiliation: South Bend Center for Medical Education, Indiana University School of Medicine, Notre Dame 46556.
authors.forename: D
authors.initials: D
authors.lastname: Christ
authors.forename: M
authors.initials: M
authors.lastname: Goebel
authors.forename: H J
authors.initials: HJ
authors.lastname: Saz
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D018680:Cholinergic Antagonists; D006224:Cricetinae; D004153:Dipetalonema / Q000502:physiology*; D058787:GABA-A Receptor Antagonists; D007551:Isotonic Contraction; D009119:Muscle Contraction / Q000187:drug effects; D009132:Muscles / Q000502:physiology; D011950:Receptors, Cholinergic / Q000187:drug effects; D011963:Receptors, GABA-A / Q000187:drug effects; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
chemical_list: D018680:Cholinergic Antagonists; D058787:GABA-A Receptor Antagonists; D011950:Receptors, Cholinergic; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid; D000109:Acetylcholine