Detection of antigens in urine of patients with acute falciparum and vivax malaria infections.
Using an antigen-capture, dot-blot assay, antigens were detected in the urine of 50 patients infected with Plasmodium falciparum. Antigens were also detected in 12/15 patients who had no detectable parasitemias 1-2 weeks after chemotherapy. By Western blotting and immunoprecipitation, four predominant antigens were identified with the following molecular masses (Mr) and isoelectric points (pI): antigen 1, 200 kDa, pI 6.4-6.27; antigen 2, 180 kDa, pI 5.2-4.8; antigen 3, 150 kDa, pI 5.5; antigen 4, 96 kDa, pI 5.1-4.8. These antigens were heat stable to 100 degrees C for 5 min. Antigens were also detected in the urine of 35 patients with acute P. vivax infections by Western blotting and dot-blot analysis and 10/10 patients three weeks following chemotherapy. The antigens had Mr of 200, 170, and 130 kDa.
Katzin
A M
AM
Departamento de Parasitologia, Universidad de Sao Paulo, Brazil.
Kimura
E S
ES
Alexandre
C O
CO
Ramos
A M
AM
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000652:urine*; D015153:Blotting, Western; D004355:Drug Stability; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006358:Hot Temperature; D006801:Humans; D015151:Immunoblotting; D016778:Malaria, Falciparum / Q000652:urine*; D016780:Malaria, Vivax / Q000652:urine*; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010963:Plasmodium falciparum / Q000276:immunology*; D010966:Plasmodium vivax / Q000276:immunology*; D011233:Precipitin Tests
D000953:Antigens, Protozoan