title: A regulated RNA binding protein also possesses aconitase activity.
abstract: A clone for the iron-responsive element (IRE)-binding protein (IRE-BP) has been transfected and expressed in mouse fibroblasts. The IRE-BP gene product binds IREs with high affinity and specificity. Amino acid alignments reveal that the IRE-BP is 30% identical to mitochondrial aconitase. The 18 active site residues of mitochondrial aconitase are identical to those in the IRE-BP, suggesting that the IRE-BP may possess aconitase activity. After purification of native IRE-BP and immunoaffinity purification of transfected and expressed IRE-BP, we demonstrate that the purified IRE-BP has aconitase activity.
authors.affiliation: Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
authors.forename: S
authors.initials: S
authors.lastname: Kaptain
authors.forename: W E
authors.initials: WE
authors.lastname: Downey
authors.forename: C
authors.initials: C
authors.lastname: Tang
authors.forename: C
authors.initials: C
authors.lastname: Philpott
authors.forename: D
authors.initials: D
authors.lastname: Haile
authors.forename: D G
authors.initials: DG
authors.lastname: Orloff
authors.forename: J B
authors.initials: JB
authors.lastname: Harford
authors.forename: T A
authors.initials: TA
authors.lastname: Rouault
authors.forename: R D
authors.initials: RD
authors.lastname: Klausner
mesh_terms: D000154:Aconitate Hydratase / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002678:Chimera; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005293:Ferritins / Q000378:metabolism; D016259:Genes, myc; D006801:Humans; D035925:Iron-Regulatory Proteins; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D035925:Iron-Regulatory Proteins; D016601:RNA-Binding Proteins; D004247:DNA; D005293:Ferritins; D000154:Aconitate Hydratase