Natural killer (NK) activity of porcine blood lymphocytes against allogeneic melanoma target cells.
Allogeneic PM/86 melanoma cells of Munich Troll miniature swine have been used for the demonstration of porcine peripheral blood NK cell activity. Compared with the specific lysis of xenogeneic K562-, U937- and Vero-target cells, NK cell-mediated cytotoxicity (NK-CMC) against PM/86 melanoma tumor cells was significantly lower in a 16 h chromium release assay. The target cell susceptibility to peripheral blood NK-CMC of both adult Troll miniature swine and German Landrace sows was very similar. Cold target inhibition assays revealed the allogeneic PM/86 melanoma cells to be the most powerful inhibitors of NK-CMC. Nylon wool non-adherent lymphocytes produced interferon (IFN)-alpha in different quantities upon contact with NK susceptible target cells. The NK effector cells could be stimulated to a higher lytic activity against all susceptible targets by a moderate dose of natural human interleukin-2 (nhuIL-2). The role of NK-CMC in melanoma tumor rejection and/or prevention of metastases is yet unknown in swine although porcine melanoma serves as a good model for the disease in man.
Büttner
M
M
Institute for Medical Microbiology, Epidemiology and Infectious Diseases, München, FRG.
Wanke
R
R
Obermann
B
B
D000818:Animals; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D005260:Female; D016898:Interferon-alpha / Q000096:biosynthesis; D007376:Interleukin-2 / Q000494:pharmacology; D007519:Isoantigens / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D008545:Melanoma / Q000276:immunology*; D012878:Skin Neoplasms / Q000276:immunology*; D013552:Swine; D013556:Swine, Miniature; D014407:Tumor Cells, Cultured
D016898:Interferon-alpha; D007376:Interleukin-2; D007519:Isoantigens