Effect of ascorbic acid, sodium citrate, sodium bicarbonate, and their combination effect on the binding of 59Fe2+ and 59Fe3+ to plasma membrane of lactating mouse mammary gland.
Isolated plasma membranes of lactating mouse mammary gland were treated with different concentrations of ascorbate, sodium citrate, sodium bicarbonate, and combinations of them (from 16 x 10(-10) to 4 x 10(-6) moles/L) and studied for the binding of 59Fe2+ and 59Fe3+ at pH 7.4. The results show that the Fe3+ form of iron is under a greater influence of anions used in these experiments. The Fe2+ form of iron is weakly bounded and affected. It is suggested that the form with a greater positive electric charge is more effectively bound to the receptors in plasma membranes.
Moutafchiev
D A
DA
Department of Biochemistry, Medico-Biological Institute, Sofia, Bulgaria.
Sirakov
L M
LM
Ovcharoff
V A
VA
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D001639:Bicarbonates / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D002951:Citrates / Q000494:pharmacology*; D019343:Citric Acid; D005260:Female; D005290:Ferric Compounds / Q000378:metabolism*; D005296:Ferrous Compounds / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007774:Lactation; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D012964:Sodium / Q000494:pharmacology*; D017693:Sodium Bicarbonate
D001639:Bicarbonates; D002951:Citrates; D005290:Ferric Compounds; D005296:Ferrous Compounds; D019343:Citric Acid; D017693:Sodium Bicarbonate; D012964:Sodium; D001205:Ascorbic Acid