The effect of the renin inhibitor ES-1005 on the expression of the kidney renin gene in sodium-depleted marmosets.
The effect of the renin inhibitor ES-1005 or captopril on the expression of the kidney renin gene was investigated in sodium-depleted marmosets. We measured the level of kidney renin messenger RNA (mRNA) after continuous administration of ES-1005 (48 mg/kg per day) or captopril (2 mg/kg per day) intraperitoneally, via an osmotic mini-pump, for one week. The level of kidney renin mRNA was measured by densitometric Northern blot analysis using an alpha-32P-labelled human renin cDNA fragment as the hybridization probe. Captopril treatment markedly increased plasma renin activity and the level of kidney renin mRNA by 4.7-fold and 6.3-fold, respectively. ES-1005 treatment completely inhibited plasma renin activity and significantly decreased the level of kidney renin mRNA (46% of the normal control P less than 0.01). However, plasma immunoreactive renin concentration was significantly increased by the treatment with ES-1005 (P less than 0.05). These results suggest that the treatment with the renin inhibitor ES-1005 for one week has a paradoxical effect on kidney renin gene expression and renin release from the kidney in sodium-depleted marmosets.
Kitami
Y
Y
2nd Department of Internal Medicine, Ehime University School of Medicine, Japan.
Hiwada
K
K
Murakami
E
E
Muneta
S
S
Kokubu
T
T
D000818:Animals; D015152:Blotting, Northern; D002143:Callitrichinae / Q000378:metabolism*; D002216:Captopril / Q000494:pharmacology; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D007668:Kidney / Q000378:metabolism; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000187:drug effects*; D012083:Renin / Q000235:genetics*; D012964:Sodium / Q000172:deficiency*; D000092502:Renin Inhibitors
D002216:Captopril; D009842:Oligopeptides; D012333:RNA, Messenger; D012083:Renin; D012964:Sodium; D000092502:Renin Inhibitors; C056080:ES 1005