An improved synthesis of carbon-11 labeled acetoacetic acid and an evaluation of its potential for the investigation of cerebral pathology by positron emission tomography.
1-11C-acetoacetic acid was synthesized by carboxylation of the acetone carbanion. Purification was carried out using HPLC. The product was obtained with a radiochemical yield of up to 58%, corrected for decay, in a total preparation time of 30 min. The distribution of 1-11C-acetoacetic acid after injection into adult Wistar rats and cats was investigated by PET. When the tracer was injected into cats, 3 weeks after inflicting a unilateral freezing lesion upon the brain, accumulation of 1-11C-acetoacetic acid in the ipsilateral brain hemisphere was observed.
Prenen
G H
GH
Department of Neurosurgery, University Hospital Groningen, The Netherlands.
Go
K G
KG
Zuiderveen
F
F
Paans
A M
AM
Vaalburg
W
W
D000090:Acetoacetates / Q000138:chemical synthesis*; D000818:Animals; D002250:Carbon Radioisotopes; D002415:Cats; D002540:Cerebral Cortex / Q000000981:diagnostic imaging*; D005260:Female; D007553:Isotope Labeling / Q000379:methods*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014055:Tomography, Emission-Computed
D000090:Acetoacetates; D002250:Carbon Radioisotopes; C016635:acetoacetic acid