Formation of the 8-hydroxydeoxyguanosine moiety in hepatic DNA of mice orally administered with luteoskyrin, a bis-anthraquinoid mycotoxin.
Following oral administration of luteoskyrin (LS), a bis-anthraquinone and hepatocarcinogenic mycotoxin of Penicillium islandicum Sopp, to ddY male mice, significant increases in serum GOT and GPT activities, 8-hydroxydeoxyguanosine (8-OH-dG) residues in hepatic DNA, and hepatic lipid peroxide level were observed. alpha-Tocopherol (alpha-TP) depressed the hepatic lipid peroxide content but did not decrease the 8-OH-dG level. These findings indicate that hydroxy radicals derived from LS are involved in the peroxidation of hepatic lipids followed by liver injury, and that the hydroxylation reaction of deoxyguanosine (dG) residues of hepatic DNA at the 8-position was unsusceptible to alpha-TP.
Masuda
T
T
Toxicology Laboratory of Lederle Ltd., Tokyo, Japan.
Miyasaka
N
N
Kato
T
T
Ueno
Y
Y
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000378:metabolism*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D009183:Mycotoxins / Q000633:toxicity*; D009285:Naphthoquinones / Q000633:toxicity*; D014810:Vitamin E / Q000494:pharmacology
D008054:Lipid Peroxides; D009183:Mycotoxins; D009285:Naphthoquinones; D014810:Vitamin E; C100224:luteoskyrin; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine