title: Effect of ascorbic acid, sodium citrate, sodium bicarbonate, and their combination effect on the binding of 59Fe2+ and 59Fe3+ to plasma membrane of lactating mouse mammary gland.
abstract: Isolated plasma membranes of lactating mouse mammary gland were treated with different concentrations of ascorbate, sodium citrate, sodium bicarbonate, and combinations of them (from 16 x 10(-10) to 4 x 10(-6) moles/L) and studied for the binding of 59Fe2+ and 59Fe3+ at pH 7.4. The results show that the Fe3+ form of iron is under a greater influence of anions used in these experiments. The Fe2+ form of iron is weakly bounded and affected. It is suggested that the form with a greater positive electric charge is more effectively bound to the receptors in plasma membranes.
authors.affiliation: Department of Biochemistry, Medico-Biological Institute, Sofia, Bulgaria.
authors.forename: D A
authors.initials: DA
authors.lastname: Moutafchiev
authors.forename: L M
authors.initials: LM
authors.lastname: Sirakov
authors.forename: V A
authors.initials: VA
authors.lastname: Ovcharoff
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D001639:Bicarbonates / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D002951:Citrates / Q000494:pharmacology*; D019343:Citric Acid; D005260:Female; D005290:Ferric Compounds / Q000378:metabolism*; D005296:Ferrous Compounds / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007774:Lactation; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D012964:Sodium / Q000494:pharmacology*; D017693:Sodium Bicarbonate
chemical_list: D001639:Bicarbonates; D002951:Citrates; D005290:Ferric Compounds; D005296:Ferrous Compounds; D019343:Citric Acid; D017693:Sodium Bicarbonate; D012964:Sodium; D001205:Ascorbic Acid