[Presynaptic augmentation induced by NaF in sympathetic ganglion of bullfrog].
Effects of NaF on the synaptic transmission of bullfrog sympathetic ganglia were studied by extra- and intracellular recordings. The results obtained were as follows: 1) The amplitude of the orthodromic compound action potential (CAP) evoked by preganglionic nerve stimulation was remarkably augmented with 10 microM NaF, whereas that of the antidromic CAPs remained unchanged with the same dose of NaF. The low amplitude of the orthodromic CAP which was diminished by a low-Ca2(+)-ringer's solution reversed with an additional administration of NaF. The amplitudes of the orthodromic CAPs were enhanced by phosphodiesterase inhibitors such as isobutylmethylxanthine, theophylline, and physostigmine. In addition, augmentation of the orthodromic CAPs was induced by an adenylate cyclase activator (forskolin) and d.b-cAMP; however, its augmented responses were not affected by an additional administration of NaF. 2) In the intracellular recording, NaF showed no effect on the resting membrane potential and depolarizing response induced by acetylcholine. However, the EPSP appearing in the phase of afterhyperpolarization of the orthodromic action potential was significantly increased by NaF, whereas no effect was found on the antidromic action potential. In order to evaluate these findings, effects of NaF on the decreased low-Ca2(+)-action potential were observed. After application of NaF, the low-Ca2(+)-orthodromic EPSPs were reversed, and when the height of the EPSP was raised to the critical firing level, a spike potential was driven in the cell. These facts suggest that the site of NaF action seems to exist in the presynaptic rather than postsynaptic process. Furthermore, it suggests that NaF probably acts on Gs-protein which activates adenylate cyclase at the presynaptic membrane. This resulted in a great increase in intracellular cAMP at the synaptic terminal and it triggered the Ca2(+)-increase. As an inevitable consequence, release of transmitter from the nerve terminals of the frog sympathetic ganglion was finally facilitated. These factors supposedly resulted in augmentation of the amplitude of the orthodromic CAP.
Sawano
H
H
Department of Oral Physiology, School of Dentistry, Iwate Medical University, Japan.
D000200:Action Potentials / Q000187:drug effects; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000502:physiology; D000242:Cyclic AMP / Q000378:metabolism; D005728:Ganglia, Sympathetic / Q000502:physiology*; D066298:In Vitro Techniques; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D011892:Rana catesbeiana; D012969:Sodium Fluoride / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013569:Synapses / Q000187:drug effects*; D009435:Synaptic Transmission / Q000187:drug effects*
D010726:Phosphodiesterase Inhibitors; D012969:Sodium Fluoride; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D002118:Calcium