title: The detection of antibodies to the glycoprotein X antigen of pseudorabies virus.
abstract: The persistence of antibodies to glycoprotein X (gpX) in the serum of pigs experimentally infected with pseudorabies virus (PRV) was determined using an anti-gpX enzyme-linked immunosorbent assay (ELISA). Antibodies to gpX were detected for at least 365 days postchallenge in nonvaccinated pigs. Previous sensitization of pigs by vaccination with S/PRV had no apparent effect on the antibody response of pigs to gpX postchallenge. In determining previous exposure of pigs to PRV strains containing the gpX gene, the anti-gpX ELISA was highly specific, but its sensitivity was lower than the standard serological procedures currently used for detecting PRV antibodies.
authors.affiliation: Veterinary Diagnostic Laboratory, Iowa State University, Ames 50010.
authors.forename: D
authors.initials: D
authors.lastname: Cook
authors.forename: H
authors.initials: H
authors.lastname: Hill
authors.forename: M
authors.initials: M
authors.lastname: Snyder
authors.forename: P
authors.initials: P
authors.lastname: McMahon
authors.forename: D
authors.initials: D
authors.lastname: Kinker
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D009500:Neutralization Tests; D011237:Predictive Value of Tests; D011557:Pseudorabies / Q000276:immunology*; D015531:Radioimmunoprecipitation Assay; D013552:Swine; D013553:Swine Diseases / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary; D014759:Viral Envelope Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C042966:pseudorabies virus glycoproteins