Evaluation of solutions for small intestinal preservation. Biochemical changes as a function of storage time.
A number of organ preservation solutions have been formulated to slow the inevitable progression of ischemic injury, thus prolonging the storage time between removal and implantation. As adenine nucleotide content has been shown to correlate with the functional recovery of transplanted livers and hearts, this study investigated the effects of 24 hr of storage in three preservation solutions, saline (SA), Euro-Collins (CO), and University of Wisconsin (UW) on adenine and nicotinamide adenine nucleotides and inosine content of the rat small intestine. Significant biochemical differences were found between segments as early as after the initial perfusion when the inosine content was higher in UW-perfused than CO- or SA-perfused segments. After 2 hr of storage in CO solution and after 6 and 24 hr in both CO and UW solutions, the ATP content was higher than in SA-stored segments. In addition to inosine, which was significantly higher at all time points for UW-stored segments, the AMP and total adenine nucleotide content of UW-stored segments at 24 hr was significantly higher than SA- or CO-stored segments. After 24 hr of storage, those segments stored in UW were able to utilize significantly more oxygen than SA-stored. These data provide biochemical evidence supporting the advantages of CO and UW storage solutions over SA for preservation of small intestine segments.
Fabian
M A
MA
Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710.
Bollinger
R R
RR
Wyble
C W
CW
Watkins
W D
WD
Canada
A T
AT
D000227:Adenine Nucleotides / Q000032:analysis; D000241:Adenosine; D000493:Allopurinol; D000704:Analysis of Variance; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005978:Glutathione; D006982:Hypertonic Solutions; D007288:Inosine / Q000032:analysis; D007328:Insulin; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D011310:Preservatives, Pharmaceutical; D011887:Raffinose; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride; D012996:Solutions; D013997:Time Factors
D000227:Adenine Nucleotides; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; D011310:Preservatives, Pharmaceutical; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D012965:Sodium Chloride; D007288:Inosine; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose