Anti-inflammatory lipocortin 1 production by peripheral blood leucocytes in response to hydrocortisone.
The presence and amount of the anti-inflammatory protein lipocortin 1 was determined in plasma and peripheral blood leucocytes by a highly specific, enzyme-linked immunosorbent assay. Within 120 min of a single intravenous dose of 100 mg hydrocortisone, the intracellular concentrations of lipocortin 1 in peripheral monocytes in 7 of 8 healthy men increased by a median of 225% (range 129-507%) compared with pretreatment levels, and mononuclear cell-surface lipocortin increased by a median of 224% (range 76-483%). Placebo injections had no effect. There was no increase at any time in free plasma or polymorph-associated lipocortin. In 3 of 4 subjects, induction of lipocortin was also observed when whole unseparated blood was incubated in vitro after steroid administration, but cells which had first been isolated and purified were refractory to such induction. Thus rapid changes in the concentration of an active anti-inflammatory protein can occur in man after normal therapeutic doses of hydrocortisone.
Goulding
N J
NJ
Bath Institute for Rheumatic Diseases, University of Bath, UK.
Godolphin
J L
JL
Sharland
P R
PR
Peers
S H
SH
Sampson
M
M
Maddison
P J
PJ
Flower
R J
RJ
D000328:Adult; D017302:Annexins; D002135:Calcium-Binding Proteins / Q000096:biosynthesis* / Q000097:blood; D004341:Drug Evaluation; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006854:Hydrocortisone / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D066298:In Vitro Techniques; D007275:Injections, Intravenous; D007962:Leukocytes / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000032:analysis; D009504:Neutrophils / Q000032:analysis; D013997:Time Factors
D017302:Annexins; D002135:Calcium-Binding Proteins; D006854:Hydrocortisone