Targeting of ricin A chain into pea chloroplasts.
A chimaeric gene was constructed encoding the pre-sequence of the 33 kDa oxygen-evolving complex protein from wheat (a thylakoid lumen protein) linked to ricin A chain. The fusion protein is efficiently imported by isolated pea chloroplasts and localised partly in the stroma, with the remainder bound to the stromal surface of the thylakoids. The imported protein is fully processed by both the stromal and thylakoidal processing peptidases, indicating that partial or complete translocation across the thylakoid membrane has taken place.
Roberts
L M
LM
Department of Biological Sciences, University of Warwick, Coventry, UK.
Meadows
J W
JW
Robinson
C
C
D000595:Amino Acid Sequence; D001692:Biological Transport; D002736:Chloroplasts / Q000737:chemistry*; D007887:Fabaceae; D017343:Genes, Plant; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D008970:Molecular Weight; D037121:Plant Lectins; D010946:Plants, Medicinal; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry; D012276:Ricin / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D014908:Triticum
D037121:Plant Lectins; D011993:Recombinant Fusion Proteins; D012276:Ricin