Enhanced detection of HIV-1 sequences using polymerase chain reaction and a liquid hybridization technique. Application for individuals with questionable HIV-1 infection.
In this report we describe a sensitive HIV-1 detection method which is applicable for confirmation testing of donors whose blood sample gives indeterminate viral-serology results. The method involves performing a polymerase chain reaction (PCR) and detecting the generated fragments using liquid hybridization and gel retardation. We found that it is as specific as blotting on a filter and hybridization with an internal probe but at least tenfold more sensitive. After applying it on DNA samples of a panel of 11 persistent indeterminate anti-p24gag-reactive donors, none was found to be PCR positive. Considering other negative virological and biochemical test results and case-historical data, these donors are not likely to be HIV-1 infected.
Bruisten
S M
SM
Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Department of Developmental Research, Amsterdam.
Koppelman
M H
MH
van der Poel
C L
CL
Huisman
J G
JG
D000386:AIDS-Related Complex / Q000097:blood; D000163:Acquired Immunodeficiency Syndrome / Q000097:blood; D001483:Base Sequence; D015153:Blotting, Western; D004279:DNA, Viral / Q000032:analysis*; D015497:HIV-1 / Q000737:chemistry*; D006801:Humans; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction
D004279:DNA, Viral