Identification of F strain Mycoplasma gallisepticum isolates by detection of an immunoreactive protein.
Commercial laying hens were examined microbiologically at necropsy 31 or 42 weeks after aerosol vaccination with the F strain of Mycoplasma gallisepticum (MG). Mycoplasma isolates were studied in Western blots probed with polyclonal antiserum raised in rabbits to F strain immunogen. The persistence of the vaccine strain was demonstrated by detection of a 75-kilodalton immunoreactive protein, which was present in all MG isolates and thought to be a unique marker of the F strain. Use of PCA-F to probe Western blots allowed simultaneous identification of non-MG isolates, non-F strains of MG, and the F strain of MG.
Thomas
C B
CB
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706.
Sharp
P
P
Fritz
B A
BA
Yuill
T M
TM
D000336:Aerosols; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology; D015153:Blotting, Western; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007106:Immune Sera / Q000276:immunology; D009174:Mycoplasma / Q000276:immunology / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000382:microbiology / Q000662:veterinary*; D011201:Poultry Diseases / Q000382:microbiology*; D014611:Vaccination / Q000662:veterinary
D000336:Aerosols; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D007106:Immune Sera