title: Hematopoietic reconstitution after high-dose chemotherapy and autologous nonfrozen bone marrow rescue.
abstract: The kinetics of marrow engraftment were analyzed in 50 patients with acute leukemia (21), malignant lymphoma (15), and solid tumors (14) after high-dose multiagent chemotherapy followed by autologous bone marrow transplantation (ABMT) with nonfrozen bone marrow. Unseparated heparinized whole bone marrow was stored in 10% CPDA1 at 4 degrees C for 72 h, then filtered and reinfused. The median number of nucleated cells reinfused was 1.6 x 10(8)/kg (range 0.5-3.8 x 10(8)/kg). All patients had a full hematopoietic reconstitution. Median time to achieve a neutrophil count greater than 500/microliters was 20 days (range 12-39) and median time to achieve an unsupported platelet count greater than 20,000/microliters was 20 days (range 10-55). The main factor associated with delayed engraftment was the number of prior chemotherapy cycles. We conclude that high-dose chemotherapy with nonfrozen ABMT is a safe procedure, without the requirement for costly cryopreservation facilities.
authors.affiliation: Abt. Hämatologie/Onkologie, Philipps-Universität, Marburg, FRG.
authors.forename: H
authors.initials: H
authors.lastname: Köppler
authors.forename: K H
authors.initials: KH
authors.lastname: Pflüger
authors.forename: K
authors.initials: K
authors.lastname: Havemann
mesh_terms: D000208:Acute Disease; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D001853:Bone Marrow; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D003131:Combined Modality Therapy; D015925:Cryopreservation; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007951:Leukemia, Myeloid / Q000188:drug therapy / Q000382:microbiology / Q000532:radiotherapy / Q000601:surgery; D008223:Lymphoma / Q000188:drug therapy / Q000382:microbiology / Q000532:radiotherapy / Q000601:surgery
chemical_list: D000970:Antineoplastic Agents