title: Detection of small GTP-binding proteins in the outer envelope membrane of pea chloroplasts.
abstract: We found small GTP-binding proteins in the outer envelope membrane of pea chloroplasts. The proteins in this membrane were separated by SDS-PAGE, transferred to a nitrocellulose filter, and incubated with [alpha-32P]GTP. Three GTP-binding proteins with the molecular weight of 24,000 were found. Binding was prevented by 10(-8)-10(-7) M GTP or by 10(-7) M guanosine 5'-[gamma-thio]triphosphate or GDP; binding was unaffected by 10(-8)-10(-6) M ATP. Thermolysin treatment of intact chloroplasts resulted in the loss of GTP-binding activity, suggesting that these proteins were in the cytosolic side of the outer envelope membrane.
authors.affiliation: Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sasaki
authors.forename: K
authors.initials: K
authors.lastname: Sekiguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Nagano
authors.forename: R
authors.initials: R
authors.lastname: Matsuno
mesh_terms: D001667:Binding, Competitive; D002736:Chloroplasts / Q000737:chemistry* / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000187:drug effects; D008565:Membrane Proteins / Q000737:chemistry* / Q000187:drug effects; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry* / Q000187:drug effects; D010946:Plants, Medicinal; D013820:Thermolysin / Q000494:pharmacology
chemical_list: D008565:Membrane Proteins; D010940:Plant Proteins; D013820:Thermolysin; D019204:GTP-Binding Proteins