[Use of digoxigenin-labeled probes for the detection of papillomaviruses by in situ hybridization].
A technique of detection of human papillomaviruses by in situ hybridization in paraffin-embedded formalin-fixed sections of genito-anal lesions is described. The probes are labeled by incorporation of digoxigenin-labeled nucleotides. The hybrids are revealed by immunohistochemistry with an anti-digoxigenin antibody coupled with alkaline phosphatase. This technique is sensitive, cheap and may be used in routine in laboratories of pathology.
Clavel
C
C
Laboratoire Pol Bouin, I.N.S.E.R.M. U. 314, Hôpital Maison-blanche, Reims.
Binninger
I
I
Polette
M
M
Boutterin
M C
MC
Birembaut
P
P
D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D004076:Digoxigenin; D006801:Humans; D007150:Immunohistochemistry; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*
D015342:DNA Probes; D004279:DNA, Viral; D004076:Digoxigenin
Utilisation de sondes marquées par la digoxigenine pour la recherche des papillomavirus par hybridation in situ.