title: The nucleotide and deduced amino acid sequences of porcine liver proline- beta-naphthylamidase. Evidence for the identity with carboxylesterase.
abstract: A cDNA clone for porcine liver proline-beta-naphthylamidase was isolated and sequenced. The deduced amino acid sequence of 567 residues was highly homologous with those of carboxylesterases (EC 3.1.1.1) previously reported for other species. In addition, proline-beta-naphthylamidase purified from porcine liver was shown to have strong activity towards p-nitrophenylacetate, a representative substrate for carboxylesterases. These results suggest that proline-beta-naphthylamidase is identical with carboxylesterase.
authors.affiliation: Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Matsushima
authors.forename: H
authors.initials: H
authors.lastname: Inoue
authors.forename: M
authors.initials: M
authors.lastname: Ichinose
authors.forename: S
authors.initials: S
authors.lastname: Tsukada
authors.forename: K
authors.initials: K
authors.lastname: Miki
authors.forename: K
authors.initials: K
authors.lastname: Kurokawa
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D001483:Base Sequence; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification; D008862:Microsomes, Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D011817:Rabbits; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity; D013552:Swine
chemical_list: D004247:DNA; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; D000626:Aminopeptidases