An in vitro predictive test for clinical graft-versus-host disease in allogeneic bone marrow transplant recipients.
An in vitro skin explant model has been used in an attempt to predict the severity of graft-versus-host disease (GVHD) in HLA-identical donor-recipient pairs. The skin explant model involves the use of donor lymphocytes which have been sensitized against recipient lymphocytes in vitro and then co-cultured with the recipient's skin. Thirteen patients were studied in a prospective manner and results from the skin explant model compared with the clinical status of the patient post-transplant showed good correlation (p less than 0.001). Results from T cell-depletion studies indicated a role for both CD4 and CD8 positive cells in GVHD. In conclusion the results confirmed that the skin explant assay is a useful and predictive test of GVHD in humans.
Sviland
L
L
Department of Pathology, Royal Victoria Infirmary, Newcastle upon Tyne, UK.
Dickinson
A M
AM
Carey
P J
PJ
Pearson
A D
AD
Proctor
S J
SJ
D000328:Adult; D001681:Biological Assay / Q000379:methods; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D046508:Culture Techniques; D006086:Graft vs Host Disease / Q000175:diagnosis* / Q000209:etiology; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000601:surgery*; D015470:Leukemia, Myeloid, Acute / Q000601:surgery*; D007959:Lymphocyte Culture Test, Mixed; D008212:Lymphocyte Depletion; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000601:surgery*; D011237:Predictive Value of Tests; D011446:Prospective Studies; D012867:Skin / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology