Immunomagnetic depletion of CD6+ cells from bone marrow and peripheral blood.
Depletion of donor CD6+ cells in HLA-identical allogeneic bone marrow transplantation has been reported to reduce graft-versus-host disease without interfering with engraftment. We have established an immunomagnetic cell separation technique capable of producing a 2-3 log depletion of CD6+ cells. Median recovery of CD6- cells and hematopoietic progenitor cells was 65-70%, and cell viability was unaffected. Significant numbers of CD2+, CD3+ cells responsive to phytohemagglutinin (PHA), OKT3, recombinant interleukin-2 (rIL-2), and allogeneic cells remained after depletion, and the number of cells able to respond to stimulation with PHA and IL-2 in vitro was reduced by only 1-2 log. These observations are not easily reconciled with the ability of CD6 depletion to prevent GVHD, but raise the question whether the depletion causes a sufficient reduction of the T cell load or removes a critical T cell subset.
Egeland
T
T
Institute of Transplantation Immunology, Rikshospitalet University Hospital, Oslo, Norway.
Albrechtsen
D
D
Martin
P J
PJ
Hansen
J A
JA
D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001854:Bone Marrow Cells; D018801:CD2 Antigens; D002469:Cell Separation / Q000379:methods; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000032:analysis; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008212:Lymphocyte Depletion; D008280:Magnetics; D011971:Receptors, Immunologic / Q000032:analysis; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; C062279:CD6 antigen; D011971:Receptors, Immunologic