[Biosensors for the determination of phenol and benzoate on the basis of Rhodococcus cells and enzyme extracts].
An amperometric biosensor for determination of phenol, cresol, benzoate and 2-methyl-4-chlorophenol using Rhodococcus-cells and enzyme extracts of Rhodococcus has been developed. The influence of cultivation of Rhodococcus-cells and preincubation of the biosensor with desired substrate on sensibility and specificity was been investigated. In relation to cultivation and preincubation the Rhodococcus sensor was high specific to benzoate or phenol and cresol. A linear range was obtained for phenol and benzoate up to 80 mumol and for 2-methyl-4-chlorophenol up to 400 mumol. The biosensor using enzyme extracts show a higher specificity, it is but necessary NADPH. A further disadvantage is the little measuring range of this sensor.
Riedel
K
K
Zentralinstitut für Molekularbiologie, Berlin-Buch.
Hensel
J
J
Ebert
K
K
D001565:Benzoates / Q000032:analysis* / Q000494:pharmacology; D015374:Biosensing Techniques; D002138:Calibration; D004798:Enzymes; D019800:Phenol; D010636:Phenols / Q000032:analysis* / Q000494:pharmacology; D011237:Predictive Value of Tests; D012240:Rhodococcus / Q000187:drug effects*
D001565:Benzoates; D004798:Enzymes; D010636:Phenols; D019800:Phenol
Biosensoren zur Bestimmung von Phenol und Benzoat auf der Basis von Rhodococcus-Zellen und Enzymextrakten.