Diagnosis by ELISA of bovine abortion due to Campylobacter fetus.
An enzyme-linked immunosorbent assay (ELISA) was developed to detect antigen-specific secretory IgA antibody in bovine vaginal mucus after abortion due to Campylobacter fetus subsp venerealis. Abortions were diagnosed by isolating the organism from 8 foetuses and/or foetal membranes and by histopathology. Vaginal mucus was collected from 7 cows shortly after abortion. All showed a high level of IgA antibody in their vaginal mucus when they were compared with an uninfected control group. The new ELISA is simple and practical and provides a useful tool for diagnosis of bovine venereal campylobacteriosis.
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Department of Agriculture and Fisheries, Regional Veterinary Laboratory, Armidale, New South Wales.
Stephens
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Quinn
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D000034:Abortion, Veterinary / Q000175:diagnosis*; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D002169:Campylobacter Infections / Q000175:diagnosis / Q000662:veterinary*; D002168:Campylobacter fetus / Q000276:immunology* / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D009093:Mucus / Q000276:immunology / Q000382:microbiology; D011247:Pregnancy; D014621:Vagina / Q000276:immunology / Q000382:microbiology
D000907:Antibodies, Bacterial; D007071:Immunoglobulin A, Secretory