Preparation and handling of aequorin solutions for the measurement of cellular Ca2+.
Main characteristics of the various types of aequorin presently available for measuring cellular Ca2+, i.e. heterogeneous aequorin, isoaequorins, recombinant aequorin, fluorescein-labeled aequorin and semi-synthetic aequorins, are summarized. Basic techniques of preparing and handling the solutions of those aequorins for measuring Ca2+, including such techniques as concentrating aequorin solutions, freeze-drying, changing buffer composition, and the regeneration of active aequorin, are described.
Shimomura
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Marine Biological Laboratory, Woods Hole, Massachusetts.
D000331:Aequorin / Q000737:chemistry; D002021:Buffers; D002118:Calcium / Q000032:analysis* / Q000378:metabolism; D004355:Drug Stability; D004356:Drug Storage; D005612:Freeze Drying; D007536:Isomerism; D008163:Luminescent Measurements; D008970:Molecular Weight; D011994:Recombinant Proteins
D002021:Buffers; D011994:Recombinant Proteins; D000331:Aequorin; D002118:Calcium