[Preliminary chromosomal localization of cloned DNA fragments from a highly metastatic human giant cell carcinoma of lung].
By using DNA transfecting and molecular cloning techniques, a 2.3 kb DNA fragment was isolated and subcloned into pUC-18 to generate pLC-2. Chromosome in situ hybridization was conducted between biotin-labelled pLC-2 and PG cell's chromosomes and the cloned gene fragment of 2.3 kb was preliminarily localized.
Zheng
W L
WL
Department of Pathology, Pejing Medical University.
D000818:Animals; D001710:Biotin; D018288:Carcinoma, Small Cell / Q000235:genetics / Q000556:secondary*; D002875:Chromosomes / Q000737:chemistry*; D003001:Cloning, Molecular; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000556:secondary*; D051379:Mice; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014162:Transfection; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D004273:DNA, Neoplasm; D001710:Biotin