A reverse approach to 1H-n.m.r. assignments of bacterial polysaccharides.
A new approach is reported for obtaining the assignment of the 1H-n.m.r. spectra of bacterial polysaccharides which are not amenable to analysis using conventional strategies. An unambiguous assignment of the 13C-n.m.r. spectrum is made by 13C-COSY of the polysaccharide labelled to a high level with 13C. Assignment of the 1H-n.m.r. spectrum is then made by proton-detected CH-correlation spectroscopy of material that is labelled at a low level. Using this approach, virtually complete assignments have been made for the Klebsiella K3 serotype polysaccharide under physiological conditions of temperature and pH.
Jones
D N
DN
University Chemical Laboratory, Cambridge, Great Britain.
Sanders
J K
JK
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D007711:Klebsiella pneumoniae / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D011522:Protons
D002247:Carbon Isotopes; D011135:Polysaccharides, Bacterial; D011522:Protons