title: [High velocity mechanical injection of foreign DNA into fish oocytes].
abstract: High-velocity tungsten microprojectiles were used to introduce into fertilized eggs of loach (Misgurnus fossilis), Rainbow trout (Salmo gairdneri Rich.) and Zebra fish (Brachydanio rerio) DNA sequences of beta-galactosidase and neomycin phosphotransferase genes. No more than 30% of fish oocytes died as a result of bombardment. Experiments revealed marked activity of both enzymes in developing fishes. Neo gene DNA sequences were found in total danio DNA using PCR technique.
authors.forename: V A
authors.initials: VA
authors.lastname: Kolesnikov
authors.forename: A A
authors.initials: AA
authors.lastname: Alimov
authors.forename: V A
authors.initials: VA
authors.lastname: Barmintsev
authors.forename: A O
authors.initials: AO
authors.lastname: Beniumov
authors.forename: I A
authors.initials: IA
authors.lastname: Zelenina
authors.forename: A M
authors.initials: AM
authors.lastname: Krasnov
authors.forename: R
authors.initials: R
authors.lastname: Dzhabur
authors.forename: A V
authors.initials: AV
authors.lastname: Zelenin
mesh_terms: D000818:Animals; D003531:Cypriniformes / Q000235:genetics*; D004247:DNA / Q000008:administration & dosage*; D019868:Kanamycin Kinase; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism; D010770:Phosphotransferases / Q000235:genetics; D014337:Trout / Q000235:genetics*; D014414:Tungsten; D015027:Zebrafish / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D004247:DNA; D010770:Phosphotransferases; D019868:Kanamycin Kinase; D001616:beta-Galactosidase; D014414:Tungsten
vernacular_title: Vysokoskorostnaia mekhanicheskaia in'ektsiia chuzherodnoĭ DNK v iaĭtsekletki ryby.