title: Minisatellite DNA profiles: rapid sample identification in linkage analysis.
abstract: Locus-specific minisatellite probes detect multiple alleles with heterozygosities of greater than 90% when hybridised to HinfI and AluI restriction digests of human DNA. We have hybridised 4 of these probes to a panel of DNAs digested with 6 of the restriction enzymes which commonly reveal di-allelic polymorphisms in the human genome. All 6 enzymes detected multiple resolvable alleles with at least 1 of the 4 minisatellite probes tested, thus providing a rapid and efficient means to check family relationships and paternity on filters already being used for linkage analysis.
authors.affiliation: Section of Human Cancer Genetics, Institute of Cancer Research, Surrey, UK.
authors.forename: H
authors.initials: H
authors.lastname: Telenius
authors.forename: J
authors.initials: J
authors.lastname: Clark
authors.forename: E
authors.initials: E
authors.lastname: Marcus
authors.forename: N
authors.initials: N
authors.lastname: Royle
authors.forename: A J
authors.initials: AJ
authors.lastname: Jeffreys
authors.forename: B A
authors.initials: BA
authors.lastname: Ponder
authors.forename: C G
authors.initials: CG
authors.lastname: Mathew
mesh_terms: D015139:Blotting, Southern; D004276:DNA, Satellite; D005060:Europe; D005554:Forensic Medicine / Q000379:methods; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004276:DNA, Satellite; D005819:Genetic Markers