title: Applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosome aberrations.
abstract: Dual color FISH with whole chromosome and pan-centromere probes facilitates rapid detection of stable structural aberrations such as translocations. This approach should allow analysis of translocations for assessment of genetic damage at long times after exposure or as a result of chronic exposure during a long period of time. Multi-color FISH with locus specific probes allows assessment of the frequency of cells carrying specific aberrations known to be associated with tumorigenesis, analysis of the series of genetic changes that occur during tumor evolution and correlation between genotype and phenotype. The power of FISH for analysis of random and tumor related events will increase steadily as informative probes are developed during the course of the International Human Genome Project.
authors.affiliation: Biomedical Sciences Division, Lawrence Livermore National Laboratory, Livermore, CA 94550.
authors.forename: J W
authors.initials: JW
authors.lastname: Gray
authors.forename: J
authors.initials: J
authors.lastname: Lucas
authors.forename: O
authors.initials: O
authors.lastname: Kallioniemi
authors.forename: A
authors.initials: A
authors.lastname: Kallioniemi
authors.forename: W L
authors.initials: WL
authors.lastname: Kuo
authors.forename: T
authors.initials: T
authors.lastname: Straume
authors.forename: D
authors.initials: D
authors.lastname: Tkachuk
authors.forename: T
authors.initials: T
authors.lastname: Tenjin
authors.forename: H U
authors.initials: HU
authors.lastname: Weier
authors.forename: D
authors.initials: D
authors.lastname: Pinkel
mesh_terms: D002869:Chromosome Aberrations; D003001:Cloning, Molecular; D005453:Fluorescence; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects; D015335:Molecular Probes; D009693:Nucleic Acid Hybridization; D011829:Radiation Dosage; D014178:Translocation, Genetic / Q000528:radiation effects
chemical_list: D015335:Molecular Probes