Level of skeletal muscle glucose transporter protein correlates with insulin-stimulated whole body glucose disposal in man.
The content of GLUT4 glucose transporter mRNA and protein were measured in samples of the vastus lateralis muscle of normal volunteers subjected to a 4-h hyperinsulinaemic, euglycaemic clamp. Plasma glucose concentration was clamped at 5.3 +/- 0.1 mmol/l, and serum insulin concentration was maintained at 740 +/- 5 pmol/l. Whole body glucose uptake averaged 38.3 +/- 2.2 mumol.kg-1.min-1, 62% of this being due to disposal via non-oxidative pathways. A significant correlation existed between basal levels of GLUT4 protein and the rate of whole body glucose disposal (r = 0.77, p less than 0.02) and non-oxidative glucose disposal (r = 0.80, p less than 0.02). There was no correlation between GLUT4 protein content and oxidative glucose disposal (r = 0.08, NS). These observations are consistent with an important role for skeletal muscle GLUT4 protein in whole body glucose disposal.
Koranyi
L I
LI
Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri.
Bourey
R E
RE
Vuorinen-Markkola
H
H
Koivisto
V A
VA
Mueckler
M
M
Permutt
M A
MA
Yki-Järvinen
H
H
D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D002151:Calorimetry; D005947:Glucose / Q000378:metabolism*; D015309:Glucose Clamp Technique; D006801:Humans; D015151:Immunoblotting; D007328:Insulin / Q000097:blood / Q000494:pharmacology*; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012016:Reference Values
D001786:Blood Glucose; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D015347:RNA Probes; D012333:RNA, Messenger; D005947:Glucose