Structural differences in 5'-flanking regions of rat cytochrome P-450aldo and P-450(11) beta genes.
Two rat genomic clones, one for cytochrome P-450aldo and the other for P-450(11) beta, were isolated and characterized. The two genes, encoding structurally homologous proteins, were closely similar in their intron-exon organizations. Their 5'-flanking regions, however, contained only a few homologous regions. A putative cyclic AMP responsive element, TGACGTGA, was found in the P-450aldo gene, but this sequence was altered at two positions in the P-450(11) beta gene. S1 nuclease protection assay revealed a single transcription initiation site for the P-450aldo gene, while multiple sites were found for the P-450(11) beta gene. These results suggest that transcriptional regulation of the rat P-450aldo and P-450(11) beta genes is due to differences in the sequences of their 5'-flanking regions.
Mukai
K
K
Department of Biochemistry, School of Medicine, Keio University, Tokyo, Japan.
Imai
M
M
Shimada
H
H
Okada
Y
Y
Ogishima
T
T
Ishimura
Y
Y
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D019405:Cytochrome P-450 CYP11B2; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005091:Exons; D005796:Genes; D015698:Genomic Library; D007438:Introns; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013252:Steroid 11-beta-Hydroxylase / Q000235:genetics*; D013250:Steroid Hydroxylases / Q000235:genetics*
D015345:Oligonucleotide Probes; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D019405:Cytochrome P-450 CYP11B2; D013252:Steroid 11-beta-Hydroxylase