New family of high-resolution ion exchangers for protein and nucleic acid purifications from laboratory to process scales.
A new family of polymer-based ion exchangers was tested for the purification of acidic and basic proteins on both the analytical and preparative scales. Protein-Pak HR series packings are available as strong cation (SP) and weak anion (DEAE) exchangers, allowing the development of a purification method regardless of the isoelectric point of protein. Three particle sizes, 8, 15 and 40 microns, are offered in scalable Advanced Purification (AP) glass columns or as bulk packings. The lower back pressures of the 15- and 40-microns packings compared to that of the 8-microns material allow rapid throughput of large volumes without exceeding the pressure limitations of the resin or the column. The capacity of the AP1 (100 mm x 10 mm) glass columns, containing these ion-exchange packings, is comparable to other ion-exchange columns. The resolution of mouse serum, plasmids, and a standard protein mixture was demonstrated and compared with the results obtained with other resin-based ion exchangers of similar particle size. Proteins were purified without significant loss of biological activity or mass.
Dion
D M
DM
Waters Chromatography, Division of Millipore Corporation, Milford, MA 01757.
O'Connor
K
K
Phillips
D
D
Vella
G J
GJ
Warren
W
W
D000476:Alkanesulfonates; D017738:Alkanesulfonic Acids; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D004983:Ethanolamines; D009696:Nucleic Acids / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification*
D000476:Alkanesulfonates; D017738:Alkanesulfonic Acids; D004983:Ethanolamines; D009696:Nucleic Acids; D011506:Proteins; C068559:propylsulfonic acid; C007369:2-diethylaminoethanol