Evaluation of the amount of nonoxynol available in condoms for the inhibition of HIV using a method based on HPLC.
A method is described for detection of nonoxynol in condoms, based on methanol-water extraction followed by reverse-phase high-performance liquid chromatography. Using this method, we found that approximately 50% of the nonionic surfactant lubricant nonoxynol migrated into elastomers (rubber latex), resulting in a concentration of nonoxynol insufficient to inhibit human immunodeficiency virus (HIV) (less than 0.05%). In order to minimize the risk of sexual transmission of HIV, and to ensure spermicidal effect and optimal rubber properties, the concentration of nonoxynol in condoms, therefore, should either be increased, or nonoxynol should be packed separately. Further studies are needed to clarify and determine the solubility and migration of nonoxynols into elastomers.
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Department of Gastroenterology, Bispebjerg Hospital, University of Copenhagen, Denmark.
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Petersen
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D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D003275:Contraceptive Devices, Male; D015658:HIV Infections / Q000517:prevention & control; D017137:Nonoxynol; D011092:Polyethylene Glycols / Q000493:pharmacokinetics*; D013089:Spermatocidal Agents / Q000493:pharmacokinetics*
D013089:Spermatocidal Agents; D017137:Nonoxynol; D011092:Polyethylene Glycols