Preliminary report: accurate assays for anti-HIV in urine.
Untreated urine specimens from 358 patients (344 attending genito-urinary medicine clinics, 14 haemophiliacs) and 353 blood donors were tested blind by a simple IgG-capture particle-adherence test (GACPAT) and a rapid IgG-capture enzyme-linked immunosorbent assay (GACELISA) for antibody to human immunodeficiency virus (anti-HIV). All 158 urine specimens from seropositive subjects were anti-HIV positive by GACPAT and 157 of them (99.4%) were positive by GACELISA. Tests on 553 urine specimens from seronegative subjects gave two repeatable false-positive reactions by GACPAT (0.4%) and none by GACELISA. By means of a modified procedure anti-gp160 was detected by commercial western blot in the urine of 44 of 45 seropositive subjects examined. IgG-capture assays will detect anti-HIV in unconcentrated urine and so allow a diagnosis in circumstances when blood sampling is impracticable.
Connell
J A
JA
PHLS Virus Reference Laboratory, London, UK.
Parry
J V
JV
Mortimer
P P
PP
Duncan
R J
RJ
McLean
K A
KA
Johnson
A M
AM
Hambling
M H
MH
Barbara
J
J
Farrington
C P
CP
D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005189:False Positive Reactions; D015483:HIV Antibodies / Q000652:urine*; D006801:Humans; D007104:Immune Adherence Reaction / Q000379:methods; D007074:Immunoglobulin G / Q000652:urine; D007158:Immunologic Techniques; D012680:Sensitivity and Specificity
D015483:HIV Antibodies; D007074:Immunoglobulin G