In vivo inhibition of superoxide production and protein kinase C activity in macrophages from vitamin E-treated rats.
Rat peritoneal macrophages, which were induced by intraperitoneal injection of vitamin E for 6 successive days, contained 387.6 to 569.0 ng alpha-tocopherol/10(6) cells and produced 0.9 to 1.5 nmol O2-/min/10(6) cells following stimulation by phorbol myristate acetate (PMA). On the other hand, control macrophages contained 1.7 ng alpha-tocopherol/10(6) cells and produced 3.9 to 4.5 nmol O2-/min/10(6) cells. The particulate fraction of macrophages from vitamin E-treated rats contained 285.2 to 294.4 ng alpha-tocopherol/10(6) cells and showed scarcely any protein kinase C activity, whereas the specific activity of control rats showed 323 to 357 pmol/min/mg protein. From these results, we concluded that vitamin E seems to decrease O2- production from macrophages stimulated with PMA through inhibition of protein kinase C.
Sakamoto
W
W
Department of Biochemistry, School of Dentistry, Hokkaido University, Sapporo, Japan.
Fujie
K
K
Handa
H
H
Ogihara
T
T
Mino
M
M
D000818:Animals; D003600:Cytosol / Q000201:enzymology; D007274:Injections, Intraperitoneal; D008264:Macrophages / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013481:Superoxides / Q000378:metabolism*; D014810:Vitamin E / Q000008:administration & dosage / Q000097:blood*
D013481:Superoxides; D014810:Vitamin E; D011493:Protein Kinase C; D010100:Oxygen