Regulation of serotonin release from enterochromaffin cells of rat cecum mucosa.
The release of endogenous serotonin or previously taken up tritiated serotonin from isolated strips of rat cecum mucosa containing enterochromaffin cells was studied in vitro. Release of tritiated serotonin was increased by potassium depolarization and was decreased by tetrodotoxin, veratridine and the absence of calcium. Endogenous serotonin was released at a lower rate than tritiated serotonin; endogenous serotonin release was stimulated by potassium depolarization but was unaffected by tetrodotoxin, veratridine or the absence of calcium. Carbachol, norepinephrine, clonidine and isoproterenol decreased release of tritiated serotonin but had less or reverse effect on release of endogenous serotonin. The results suggest two different serotoninergic pools within the enterochromaffin cell population.
Simon
C
C
INSERM U6-CNRS, URA 142, Marseille, France.
Ternaux
J P
JP
D000818:Animals; D002432:Cecum / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D002838:Chromaffin System / Q000378:metabolism*; D004759:Enterochromaffin Cells / Q000187:drug effects / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D018377:Neurotransmitter Agents / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology; D014316:Tritium; D014701:Veratridine / Q000494:pharmacology
D018377:Neurotransmitter Agents; D014316:Tritium; D012701:Serotonin; D013779:Tetrodotoxin; D014701:Veratridine; D011188:Potassium