Transforming growth factor-beta-induced gene expression of monocyte chemoattractant JE in mouse osteoblastic cells, MC3T3-E1.
A recent study demonstrated that PDGF-inducible JE is an inflammatory cytokine that directs chemotactic activity of monocytes. Accumulation of monocyte/macrophage lineage cells at site of bone tissue sites is very important for formation of multinucleate osteoclasts, which mediate bone resorption. Since transforming growth factor-beta (TGF-beta) is a potent regulator in bone remodeling, we examined whether TGF-beta induced JE gene expression in mouse osteoblastic cells, MC3T3-E1. TGF-beta induced a maximum JE mRNA expression at 3 hr after initiation of the cytokine treatment. This maximal expression was observed in when TGF-beta was used at a concentration of 1 ng/ml. The chemotactic activity for human monocytes was detected in conditioned medium of TGF-beta-treated cells, and the chemotactic activity was neutralized by anti-JE serum treatment.
Hanazawa
S
S
Department of Oral Microbiology, Meikai University School of Dentistry, Saitama, Japan.
Takeshita
A
A
Tsukamoto
Y
Y
Kawata
Y
Y
Takara
K O
KO
Kitano
S
S
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000235:genetics* / Q000502:physiology; D002634:Chemotaxis, Leukocyte; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D051379:Mice; D009000:Monocytes / Q000502:physiology; D010006:Osteoblasts; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D018932:Chemokine CCL2; D002630:Chemotactic Factors; D012333:RNA, Messenger; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta