title: Bacterial colonization and infection studies in the premature baboon with bronchopulmonary dysplasia.
abstract: Microbial colonization and infection patterns were prospectively evaluated in premature baboons with and without bronchopulmonary dysplasia (BPD) to assess if prolonged hyperoxia would predispose to a different pattern of microbial colonization and/or a higher risk of respiratory infection. Forty baboons were delivered by hysterotomy at 75% of gestation and randomized into two groups. Group I (control or PRN) animals were placed immediately on high-frequency oscillation at 15 Hz; I:E ratio 1:2, and changed to positive-pressure ventilation at 48 to 72 h. They were maintained on clinically appropriate oxygen at minimal ventilator settings for the remainder of the 21-day experimental period. Group II (oxygen-treated or BPD) animals were ventilated with PPV and FIO2 1.0 for 7 days followed by FIO2 0.8 for 14 days. All animals were treated with antibiotics during some portion of the 21-day course. Specimens from nose, oropharynx, trachea, and rectum were cultured for both aerobes and anaerobes throughout the neonatal intensive care unit (NICU) course. A subset of animals from both groups were killed at 21 days and lung, liver, spleen, and gastric contents were cultured quantitatively at autopsy. Findings showed that coagulase-negative staphylococci were the predominant organisms that colonized the neonate in the NICU. Lung infections were seen to evolve through sequential pathogenetic steps: colonization of the upper respiratory tract, with concomitant or subsequent colonization of the trachea with comparable organism and ultimate recovery of the same organisms at autopsy in the lungs of animals with pneumonia.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pathology, University of Texas Health Science Center, San Antonio 78284-7750.
authors.forename: J J
authors.initials: JJ
authors.lastname: Coalson
authors.forename: D R
authors.initials: DR
authors.lastname: Gerstmann
authors.forename: V T
authors.initials: VT
authors.lastname: Winter
authors.forename: R A
authors.initials: RA
authors.lastname: Delemos
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001420:Bacteria, Aerobic / Q000302:isolation & purification; D001421:Bacteria, Anaerobic / Q000302:isolation & purification; D001424:Bacterial Infections / Q000453:epidemiology / Q000382:microbiology; D001997:Bronchopulmonary Dysplasia / Q000382:microbiology*; D004195:Disease Models, Animal; D006801:Humans; D007231:Infant, Newborn; D010100:Oxygen / Q000008:administration & dosage; D010215:Papio / Q000382:microbiology*; D011446:Prospective Studies; D013997:Time Factors
chemical_list: D010100:Oxygen