Effect of spermatozoa selection on a simplified Percoll gradient in case of asthenozoospermia.
Two methods for preparing semen (Swim-up migration and centrifugation on a two step density Percoll gradient) were tested in 21 cases of asthenozoospermia (motility less than 30%). The parameters of sperm movement were studied through automated videomicrographic analysis. In 9 cases out of 21 swim-up migration failed while centrifugation on a Percoll gradient failed in only 1 case out of 21.
Mathieu
C
C
Laboratoire de Biologie de la Reproduction, Faculté de Médecine, Lyon/France.
Mein
M
M
Lornage
J
J
Li
J G
JG
Guerin
J F
JF
D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D007317:Insemination, Artificial, Homologous / Q000379:methods*; D008297:Male; D011205:Povidone; D012822:Silicon Dioxide; D013081:Sperm Motility; D013094:Spermatozoa / Q000473:pathology
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone