Interactions between the human monocytic leukaemia THP-1 cell line and Old and New World species of Leishmania.
The human promyelocytic THP-1 cell line has been found to support the growth of Leishmania parasites. THP-1 cells, differentiated with retinoic acid, cease replication while remaining in suspension. 72 +/- 8% of THP-1 cells became infected after inoculation with promastigotes of several Old and New World Leishmania species. The resulting amastigotes (19 +/- 5 per infected cell) were easy to harvest, capable of reinfecting cultures of normal human cells and, in the case of L. major and L. infantum, caused specific lesions in BALB/c mice. This culture system should facilitate biochemical and immunological studies on amastigotes and be of use in screening anti-parasite drugs.
Ogunkolade
B W
BW
INSERM U313, CHU Pitie-Salpêtrière, Paris, France.
Colomb-Valet
I
I
Monjour
L
L
Rhodes-Feuillette
A
A
Abita
J P
JP
Frommel
D
D
D000818:Animals; D006801:Humans; D007891:Leishmania / Q000254:growth & development* / Q000502:physiology; D007893:Leishmania donovani / Q000254:growth & development / Q000502:physiology; D007895:Leishmania tropica / Q000254:growth & development / Q000502:physiology; D007951:Leukemia, Myeloid; D016403:Lymphoma, Large B-Cell, Diffuse; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000469:parasitology*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D014212:Tretinoin