Homeodomain binding sites in the 5' flanking region of the Bombyx mori silk fibroin light-chain gene.
Multiple A + T-rich stretches in the 5' flanking region of the Bombyx mori fibroin light-chain gene have been shown to bind two Drosophila homeodomain proteins, EVE (even-skipped) and ZEN (zerknüllt), with high affinities. Some of these sites fall into a class that has the established consensus sequence of the binding sites (TCAATTAAAT) for a diverse group of Drosophila homeodomain proteins, while others are quite heterogenous except that they all possess a core TAAT motif. Since clusters of homeodomain binding sites can also be found in the promoters of other silk protein genes, the fibroin gene and the sericin-1 gene, these observations suggest a possible involvement of some homeobox genes in the regulation of a group of silk protein genes.
Hui
C C
CC
Department of Developmental Biology, National Institute for Basic Biology, Okazaki, Japan.
Suzuki
Y
Y
Kikuchi
Y
Y
Mizuno
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins; D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D012831:Bombyx / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004268:DNA-Binding Proteins / Q000235:genetics*; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005349:Fibroins / Q000235:genetics*; D005801:Genes, Homeobox; D018398:Homeodomain Proteins; D019476:Insect Proteins; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D015183:Restriction Mapping; D047011:Silk; D014157:Transcription Factors
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D029968:Escherichia coli Proteins; C474481:EspD protein, E coli; D018398:Homeodomain Proteins; D019476:Insect Proteins; D011506:Proteins; D047011:Silk; D014157:Transcription Factors; C052837:eve protein, Drosophila; D004247:DNA; D005349:Fibroins