Detection of human cytomegalovirus in cervicovaginal cells by culture, in situ DNA hybridization and DNA amplification methods.
The presence of human cytomegalovirus (HCMV) was tested in 388 cervicovaginal cells specimens obtained from the same number of pregnant women. HCMV was detected in 5.41%, 11.6% and 13.9% of these specimens by conventional culture, in situ DNA hybridization and polymerase chain reaction (PCR) methods, respectively. The sensitivities of detecting HCMV by in situ hybridization and PCR methods were 76.2% and 90.5% and the specificities were 92.1% and 90.5%, respectively, when compared with conventional culture method. The PCR compared favourably with both conventional culture and in situ hybridization methods and it may become a valuable and useful tool for the early and rapid detection of HCMV in clinical specimens.
Yuan
C F
CF
Department of Obstetrics and Gynecology, Veteran General Hospital, Taipei, Taiwan, Republic of China.
Kao
S M
SM
Wang
D C
DC
Ng
H T
HT
Pao
C C
CC
D001483:Base Sequence; D015139:Blotting, Southern; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000166:cytology / Q000382:microbiology*; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D014621:Vagina / Q000166:cytology / Q000382:microbiology*
D004279:DNA, Viral