title: The proliferative activity of B-chronic lymphocytic leukaemia lymphocytes prior to and after stimulation with TPA and PHA.
abstract: The proliferative activity of B-CLL lymphocytes from 10 patients was investigated both prior to and after stimulation with TPA and PHA. The analysis of cell cycle-associated features such as BrdU incorporation and the expression of the nuclear proliferation-associated antigen, Ki-67, together with the phenotypic profile of the cells, was performed using double colour immunofluorescent methods. The unstimulated B-CLL cells represented a homogeneous population with the same cell cycle position (G0) as resting peripheral blood lymphocytes. After TPA stimulation 22.7% of the lymphocytes were found in G1, 9.4% in S + G2/M and 13.4% in post-M. PHA stimulation induced a greater proportion of cells in G1, i.e. 35% and 17.8% into S + G2/M and 13.4% into post-M. Double colour immunofluorescence was able to demonstrate that in TPA cultures the majority of the stimulated lymphocytes originated from the malignant clone. Evidence of B-CLL lymphocyte proliferation using double colour labelling with BrdU and Ig kappa and/or Ig lambda showed that a small minority of B-CLL lymphocytes were stimulated into S + G2/M phases of the cell cycle. PHA was also capable of inducing a small proportion of B-CLL cells into mitosis although this proportion of cells was smaller compared to the TPA-stimulated lymphocytes.
authors.affiliation: Department of Haematology, Queens' University of Belfast, Northern Ireland, U.K.
authors.forename: C F
authors.initials: CF
authors.lastname: Stephenson
authors.forename: Z R
authors.initials: ZR
authors.lastname: Desai
authors.forename: J M
authors.initials: JM
authors.lastname: Bridges
mesh_terms: D000368:Aged; D001402:B-Lymphocytes / Q000187:drug effects*; D001973:Bromodeoxyuridine / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis; D010641:Phenotype; D010835:Phytohemagglutinins; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D019394:Ki-67 Antigen; D009687:Nuclear Proteins; D010835:Phytohemagglutinins; D001973:Bromodeoxyuridine; D013755:Tetradecanoylphorbol Acetate