[Detection of a circulating SJ 70 antigen in human schistosomiasis japonica using a monoclonal antibody].
A double antibody enzyme-linked immunosorbent assay (sandwich ELISA) was used for detection of a circulating antigen from human Schistosoma japonicum infections. This assay involves the use of poly clonal rabbit anti-schistosoma japonicum soluble egg antigen (SEA) antiserum to bind circulating antigen and a monoclonal antibody (MCAb-H4) to identify and quantify this antigen. Sera from 108 S. japonicum infected patients (acute and chronic) were tested. Sera from 93 of 95 (98%) patients with chronic infection were positive for this antigen; sera from 12 of 13 patients (93%) with acute infections were also positive. Antigen was not detectable in control human sera. Sera from 35 chronic schistosomiasis patients were collected between 6 and 12 months after paraziquantel treatment. Circulating antigen was not detectable in the sera from 33 of these patients (94%) and was dramatically reduced in the other two patients.
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D000293:Adolescent; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000032:analysis*; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D010063:Ovum / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth