title: Proteolytic release of keratinocyte transglutaminase.
abstract: Human keratinocytes express a particulate transglutaminase that can be released from the membrane by limited proteolysis with trypsin or plasmin to yield a form that is congruent to 80 kDa. The enzyme from cultured cells was also releasable by endogenous proteolysis to yield a catalytically active fragment of congruent to 80 kDa. Endogenous release was strongly dependent upon temperature and Ca2+ concentration and was inhibited by iodoacetate, but not by leupeptin, antipain or phenylmethanesulphonyl fluoride. These phenomena raise the possibility of partial translocation of transglutaminase activity to the cytoplasm by proteolysis to which the enzyme is subject during terminal differentiation. In addition, hydrodynamic measurements showed that the endogenously released enzyme was monomeric in solution (79 kDa), whereas that solubilized by hydroxylamine without proteolysis appeared dimeric (190 kDa). The latter dimeric state may reflect either an altered conformation of the enzyme or post-translational modification beyond fatty acid esterification.
authors.affiliation: Charles A. Dana Laboratory of Toxicology, Harvard School of Public Health, Boston, MA 02115.
authors.forename: R H
authors.initials: RH
authors.lastname: Rice
authors.forename: X H
authors.initials: XH
authors.lastname: Rong
authors.forename: R
authors.initials: R
authors.lastname: Chakravarty
mesh_terms: D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D002850:Chromatography, Gel; D005341:Fibrinolysin / Q000494:pharmacology; D006801:Humans; D007231:Infant, Newborn; D015603:Keratinocytes / Q000201:enzymology*; D008297:Male; D008970:Molecular Weight; D013696:Temperature; D011503:Transglutaminases / Q000302:isolation & purification / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology
chemical_list: D011503:Transglutaminases; D014357:Trypsin; D005341:Fibrinolysin