[Cell differentiation action of 4'-epi-adriamycin in WEHI-3B myelomonocytic leukemia cells].
We studied the effect of 4'-epi-adriamycin on the differentiation of WEHI-3B (D+) murine myelomonocytic leukemia cell line (parent line) and WEHI-3B-MIT-R subline resistant to induction of differentiation by mitoxantrone. WEHI-3B(D+) cells were differentiated into mature granulocytes in suspension culture when exposed to 4'-epi-adriamycin regardless of the cell densities. The maximum differentiation rate was produced by 50 nM 4'-epi-adriamycin; at this concentration, approximately 40% of the cell population expressed a differentiated phenotype, as assessed by their ability to reduce nitroblue tetrazolium Induction of differentiation was observed by 4'-epi-adriamycin even at low cell densities (less than 10(5)/ml) that excluded the effects of autoinduction of differentiation. WEHI-3B-MIT-R cell line, which have a capacity of G-CSF-induced differentiation, was cross-resistance to 4'-epi-adriamycin. These results suggest that the mechanism of action differs from that of the induction of differentiation by G-CSF (which is known to show the activity by the secondary autoinduction of differentiation) and the differentiation induction is due to the direct action of 4'-epi-adriamycin.
Kajigaya
Y
Y
Dept. of Pediatrics, Yokohama City University School of Medicine.
Ikuta
K
K
Sasaki
H
H
Matsuyama
S
S
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D015251:Epirubicin / Q000494:pharmacology*; D015479:Leukemia, Myelomonocytic, Acute / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
D015251:Epirubicin