title: The mitochondrial genome of an exsymbiotic Chlorella-like green alga.
abstract: Mitochondrial (mt) DNA from the unicellular, exsymbiotic Chlorella-like green alga, strain Nla was isolated and cloned. The mtDNA has a buoyant density of 1.692 g/ml in CsCl and an apparent G/C base composition of 32.5%. The genome contains approximately 76 kbp of DNA based on restriction fragment summation and electron microscopic measurements. A map of restriction endonuclease sites using Sst I, Bam I, Sal I and Xho I was generated. The genome maps as a circular molecule and appears as such under the electron microscope. Eight genes were assigned to the map by hybridization to specific restriction fragments using heterologous mt-encoded specific probes. These include the genes for subunits 6, 9, and alpha of the F0-F1 ATPase complex, the large and small subunit rRNAs, cytochrome oxidase subunits I and II, and apocytochrome b.
authors.affiliation: School of Biological Sciences, University of Nebraska-Lincoln 68588-0118.
authors.forename: J A
authors.initials: JA
authors.lastname: Waddle
authors.forename: A M
authors.initials: AM
authors.lastname: Schuster
authors.forename: K W
authors.initials: KW
authors.lastname: Lee
authors.forename: R H
authors.initials: RH
authors.lastname: Meints
mesh_terms: D001059:Apoproteins / Q000235:genetics; D001482:Base Composition; D015139:Blotting, Southern; D002708:Chlorella / Q000201:enzymology / Q000235:genetics* / Q000648:ultrastructure; D000460:Chlorophyta / Q000201:enzymology / Q000235:genetics* / Q000648:ultrastructure; D003573:Cytochrome b Group / Q000235:genetics; D045303:Cytochromes b; D004270:DNA, Circular / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D003576:Electron Transport Complex IV / Q000235:genetics; D008854:Microscopy, Electron; D006180:Proton-Translocating ATPases / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D015183:Restriction Mapping
chemical_list: D001059:Apoproteins; D003573:Cytochrome b Group; D004270:DNA, Circular; D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal; D045303:Cytochromes b; D003576:Electron Transport Complex IV; D006180:Proton-Translocating ATPases