[Establishment and characterization of new cell lines from human renal cell carcinoma].
We have characterized seven human renal cell carcinoma cell lines established from primary sites of five patients between 1987 and 1989. Two lines, OUR-20P and OUR-20S, were derived from the OUR-20 cells by cloning with a dilution method 3 months after the primary culture. These three cell lines were tumorigenic in athymic nude mice when inoculated subcutaneously. Examined by a dye uptake method, OUR-20 was highly sensitive to interferon-alpha (IFN-alpha); OUR-20P, OUR-20S and OUR-30 showed slight sensitivities, while the other three cell lines were insensitive. All seven cell lines have been maintained for more than 2 years and over 50 passages in vitro. Cytogenetic analyses performed 1.5 to 3 years after the starts of primary cultures indicated that all seven cell lines, which exhibited different morphologies in phase-contrast micrographs, were aneuploid with modal chromosome numbers 41 to 89.
Sugao
H
H
Department of Urology, Osaka University Medical School.
Seguchi
T
T
Nakano
E
E
Sonoda
T
T
Matsuda
M
M
Goto
T
T
Onishi
S
S
Kawaguchi
N
N
D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000473:pathology*; D002455:Cell Division; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology; D007621:Karyotyping; D007680:Kidney Neoplasms / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured
D016898:Interferon-alpha