title: Involvement of translation and transcription in insect steroidogenesis.
abstract: The involvement of protein and RNA synthesis in insect steroidogenesis was investigated using the prothoracic glands of the tobacco hornworm Manduca sexta. Ecdysone secretion stimulated by prothoracicotropic hormone (PTTH) and by cAMP analogs such as dibutyryl cAMP (dbcAMP), was suppressed by the translation inhibitors cycloheximide and puromycin, and by the transcription inhibitor actinomycin D. Inhibition of protein synthesis did not prevent the activation of glandular kinases, as indicated by continued protein phosphorylation in the presence of cycloheximide. Incorporation of radiolabeled amino acids and uridine increased within 60 min of glandular activation, suggesting that ecdysteroid secretion was accompanied by enhanced protein and RNA synthesis. One-dimensional gel electrophoresis revealed an increase in the translation of glandular proteins within 20 min of activation. The results suggest that the translation of protein from short-lived mRNA is necessary for optimal synthesis of ecdysteroids, and that the requisite proteins act beyond the activation of cAMP-dependent protein kinase.
authors.affiliation: Department of Biology, Northeastern University, Boston, MA 02115.
authors.forename: D A
authors.initials: DA
authors.lastname: Keightley
authors.forename: K J
authors.initials: KJ
authors.lastname: Lou
authors.forename: W A
authors.initials: WA
authors.lastname: Smith
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D004440:Ecdysone / Q000032:analysis / Q000096:biosynthesis*; D007301:Insect Hormones / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D007313:Insecta / Q000235:genetics*; D014176:Protein Biosynthesis / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D007301:Insect Hormones; D004440:Ecdysone; C009089:prothoracicotropic hormone; D003994:Bucladesine