title: Recombinant plasmid expressing the entire coding region of the Bmyc putative protein.
abstract: An expression vector with the entire coding region of Bmyc oncogene was constructed. The longest predicted open reading frame of Bmyc (178 amino acid residues) was expressed as a fusion protein with the trpE protein (308 amino acid residues) in transformed bacteria. The newly synthesized 58 Kd protein reacted with an anti pan-myc serum. The fusion protein isolated from a preparative Western blot was used as immunogen to generate rabbit anti myc specific immune sera.
authors.affiliation: Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Steinitz
authors.forename: S
authors.initials: S
authors.lastname: Ingvarsson
authors.forename: C
authors.initials: C
authors.lastname: Asker
authors.forename: J
authors.initials: J
authors.lastname: Sümegi
authors.forename: G
authors.initials: G
authors.lastname: Klein
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D015153:Blotting, Western; D004926:Escherichia coli; D016259:Genes, myc; D005822:Genetic Vectors; D007106:Immune Sera; D016366:Open Reading Frames; D010957:Plasmids; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology
chemical_list: C076792:Bmyc protein, rat; D007106:Immune Sera; D016271:Proto-Oncogene Proteins c-myc; D011993:Recombinant Fusion Proteins