Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies.
Hoffmann
A
A
Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, NY.
Roeder
R G
RG
D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors; D006639:Histidine / Q000737:chemistry*; D008969:Molecular Sequence Data; D011489:Protein Denaturation; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D011994:Recombinant Proteins; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D006639:Histidine