Resistance of Pasteurella multocida A:3,4 to phagocytosis by turkey macrophages and heterophils.
A virulent field isolate and 2 vaccine strains of Pasteurella multocida A:3,4 were compared for resistance to phagocytosis by turkey macrophages and heterophils, using in vitro assays. The least virulent vaccine strain (M-9) was phagocytosed to a greater degree than was the field isolate or the other vaccine strain (Clemson University). All 3 bacteria differed significantly from each other in the amount of capsular material present as measured by polycationic ferritin labeling and electron microscopy. Removal of the capsule with hyaluronidase resulted in a significant increase in phagocytosis of the field isolate.
Harmon
B G
BG
Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602.
Glisson
J R
JR
Latimer
K S
KS
Steffens
W L
WL
Nunnally
J C
JC
D000818:Animals; D006098:Granulocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008854:Microscopy, Electron; D009895:Opsonin Proteins; D016979:Pasteurella multocida / Q000276:immunology* / Q000472:pathogenicity / Q000648:ultrastructure; D010587:Phagocytosis; D014422:Turkeys; D014774:Virulence
D009895:Opsonin Proteins