Lipoylation of H-protein of the glycine cleavage system. The effect of site-directed mutagenesis of amino acid residues around the lipoyllysine residue on the lipoate attachment.
H-protein of the glycine cleavage system has lipoic acid on the Lys59 residue. Comparison of amino acid sequences around the lipoate attachment site of H-proteins from various sources and acyltransferases of alpha-keto acid dehydrogenase complexes indicated that Gly43, Glu56, Glu63 and Gly70 of bovine H-protein are highly conserved among these proteins. Modification of these conserved residues by site-directed mutagenesis indicated that Glu56 and Gly70 are important for the lipoylation of H-protein and suggested that the proper conformation around the lipoic acid attachment site is required for the association of H-protein to the enzyme responsible for the lipoylation.
Fujiwara
K
K
Institute for Enzyme Research, University of Tokushima, Japan.
Okamura-Ikeda
K
K
Motokawa
Y
Y
D000594:Amino Acid Oxidoreductases; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D002417:Cattle; D002645:Chickens; D005998:Glycine / Q000737:chemistry / Q000235:genetics*; D050961:Glycine Decarboxylase Complex H-Protein; D050841:Glycine Dehydrogenase (Decarboxylating); D006801:Humans; D006868:Hydrolysis; D008239:Lysine / Q000031:analogs & derivatives* / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D014176:Protein Biosynthesis; D008063:Thioctic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics*; D014158:Transcription, Genetic
D002352:Carrier Proteins; D050961:Glycine Decarboxylase Complex H-Protein; C067769:lipoyllysine; D008063:Thioctic Acid; D000594:Amino Acid Oxidoreductases; D050841:Glycine Dehydrogenase (Decarboxylating); D008239:Lysine; D005998:Glycine