title: Application of a novel chemiluminescence-based DNA detection method to single-vector and multiplex DNA sequencing.
abstract: A chemiluminescent DNA detection method is described and its application shown for both single-vector and multiplex DNA sequencing using the standard dideoxy chain-termination process. This recently developed detection method, which utilizes the light emitted by an enzyme-catalyzed dioxetane reaction, is highly sensitive and affords significant advantages in safety and speed over the traditional radioactive labeling method. When adapted to a multiplex strategy, this chemiluminescent detection method constitutes a safe, simple and rapid method for increasing the throughput of DNA sequencing procedures.
authors.affiliation: New England BioLabs.
authors.forename: A
authors.initials: A
authors.lastname: Creasey
authors.forename: L
authors.initials: L
authors.lastname: D'Angio
authors.forename: T S
authors.initials: TS
authors.lastname: Dunne
authors.forename: C
authors.initials: C
authors.lastname: Kissinger
authors.forename: T
authors.initials: T
authors.lastname: O'Keeffe
authors.forename: H
authors.initials: H
authors.lastname: Perry-O'Keefe
authors.forename: L S
authors.initials: LS
authors.lastname: Moran
authors.forename: M
authors.initials: M
authors.lastname: Roskey
authors.forename: I
authors.initials: I
authors.lastname: Schildkraut
authors.forename: L E
authors.initials: LE
authors.lastname: Sears
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005821:Genetic Techniques; D005822:Genetic Vectors / Q000235:genetics; D008163:Luminescent Measurements
chemical_list: D004247:DNA; D004259:DNA-Directed DNA Polymerase