Multiple ligand binding functions for VLA-2 (alpha 2 beta 1) and VLA-3 (alpha 3 beta 1) in the integrin family.
In our studies of VLA-3 we have shown (i) that a single integrin (VLA-3) can bind to multiple ligands by different mechanisms, involving RGD and non-RGD sites, which are regulated differently by divalent cations. Also we showed from the primary sequence of VLA-3 that it is only distantly related to the other cleaved alpha subunits. In our studies of VLA-2 we have shown that a single integrin may have at least three functional forms, depending on the cell type where expressed. In addition, we have expressed functional VLA-2 in RD cells, resulting in both Coll and Lm binding functions in vitro, and increased tumor cell metastasis in vivo in nude mice.
Hemler
M E
ME
Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115.
Elices
M J
MJ
Chan
B M
BM
Zetter
B
B
Matsuura
N
N
Takada
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D002413:Cations, Divalent / Q000378:metabolism; D002448:Cell Adhesion; D003094:Collagen / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D008024:Ligands; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism; D011487:Protein Conformation; D011956:Receptors, Cell Surface / Q000378:metabolism*; D038981:Receptors, Collagen; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000378:metabolism*; D017474:Receptors, Laminin; D018000:Receptors, Peptide; D016029:Receptors, Very Late Antigen / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism
D002413:Cations, Divalent; D005353:Fibronectins; D007797:Laminin; D008024:Ligands; D009363:Neoplasm Proteins; D009842:Oligopeptides; D011956:Receptors, Cell Surface; D038981:Receptors, Collagen; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic; D017474:Receptors, Laminin; D018000:Receptors, Peptide; D016029:Receptors, Very Late Antigen; D011994:Recombinant Proteins; C051382:arginyl-glycyl-aspartic acid directed cell adhesion receptor; C047981:arginyl-glycyl-aspartic acid; D003094:Collagen; C058161:glycyl-arginyl-glycyl-aspartyl-seryl-proline; C058347:glycyl-arginyl-glycyl-glutamyl-seryl-proline