Determination of 3'-azido-3'-deoxythymidine, 2',3'-dideoxycytidine, 3'-fluoro-3'-deoxythymidine and 2',3'-dideoxyinosine in biological samples by high-performance liquid chromatography.
A simple and fast high-performance liquid chromatographic assay for the determination of 3'-azido-3'-deoxythymidine (AZT), 2',3'-dideoxycytidine (ddC), 3'-fluoro-3'-deoxythymidine (FT) and 2',3'-dideoxy-inosine (ddI) in complex biological matrices is described. The method allows rapid nucleoside determination using a phenyl column within extra- as well as intracellular media without further sample pretreatment and extraction procedures. The lower limit of detection is ca. 0.05 micrograms/ml for each nucleoside, and the separation can easily be optimized for AZT, ddC, FT and ddI by variation of the methanolic part of the mobile phase and the detector wavelengths.
Frijus-Plessen
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Department of Pharmacology and Toxicology, University of Göttingen, F.R.G.
Michaelis
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HC
Foth
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Kahl
G F
GF
D000818:Animals; D000998:Antiviral Agents / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016049:Didanosine / Q000032:analysis; D015224:Dideoxynucleosides / Q000032:analysis* / Q000097:blood; D006801:Humans; D008099:Liver / Q000737:chemistry / Q000166:cytology; D051381:Rats; D016047:Zalcitabine / Q000032:analysis; D015215:Zidovudine / Q000032:analysis
D000998:Antiviral Agents; D015224:Dideoxynucleosides; D015215:Zidovudine; D016047:Zalcitabine; D016049:Didanosine; C002854:alovudine