Immunoblot analysis of components of Penicillium notatum recognized by human IgE antibodies.
Components of the crude extract of Penicillium notatum recognized by human IgE antibodies (Abs) were investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. The allergenic components were identified with sera from 19 allergic patients and 20 blood donors. The allergen-Ab complexes were visualized by 125I-labeled monoclonal antihuman IgE and autoradiography. A total of 11 allergenic components, ranging in molecular weights (MWs) from 94,000 to 20,000 daltons, were identified. Heterogeneity in the IgE-binding patterns of the serum samples tested was also observed. However, the major allergen appears to be the component with an MW of about 68,000 daltons that was recognized by IgE Abs in 56% of the 39 sera analyzed. Furthermore, the component with an MW of about 64,000 daltons that was recognized by IgE Abs in 46% of the sera analyzed was also considered as an important allergen. Results obtained from this study will be useful in additional characterization of allergens of P. notatum and related fungal species.
Shen
H D
HD
Department of Medical Research, Veterans General Hospital, Taipei, Taiwan, Republic of China.
Choo
K B
KB
Wang
S R
SR
Lin
W L
WL
Chang
Z N
ZN
Han
S H
SH
D000485:Allergens / Q000032:analysis; D000908:Antibodies, Fungal / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000946:Antigens, Fungal / Q000276:immunology*; D001249:Asthma / Q000276:immunology; D001345:Autoradiography; D001782:Blood Donors; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000032:analysis*; D008970:Molecular Weight; D010407:Penicillium / Q000276:immunology*
D000485:Allergens; D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D007073:Immunoglobulin E