title: Uremic serum contains humoral factor(s) larger than fifty kilodaltons which suppresses endothelin production in cultured endothelial cells.
abstract: Direct effects of human uremic serum on the production of endothelin-1 in cultured porcine endothelial cells were examined in this study. Uremic serum decreased the level of monomeric endothelin-1 secreted into the culture medium by endothelial cells. This effect occurred at a transcriptional step because uremic serum decreased the endothelin-1 mRNA level in those cells. For the partial characterization of this inhibitory activity, uremic serum was fractionated with a centricut column. Uremic serum contains humoral factor(s) larger than 50 kD which suppress the endothelin-1 mRNA level in cultured endothelial cells.
authors.affiliation: Second Department of Internal Medicine, Osaka City University Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Koyama
authors.forename: H
authors.initials: H
authors.lastname: Tahara
authors.forename: T
authors.initials: T
authors.lastname: Shoji
authors.forename: Y
authors.initials: Y
authors.lastname: Nishizawa
authors.forename: M
authors.initials: M
authors.lastname: Inaba
authors.forename: S
authors.initials: S
authors.lastname: Otani
authors.forename: M
authors.initials: M
authors.lastname: Yanagisawa
authors.forename: Y
authors.initials: Y
authors.lastname: Ishiguro
authors.forename: N
authors.initials: N
authors.lastname: Takanashi
authors.forename: H
authors.initials: H
authors.lastname: Morii
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016232:Endothelins / Q000096:biosynthesis* / Q000235:genetics; D004730:Endothelium, Vascular / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D013552:Swine; D014511:Uremia / Q000097:blood*
chemical_list: D016232:Endothelins; D012333:RNA, Messenger