Synthetic peptides against foot-and-mouth disease--immunization with VP1-peptides of type O1-Kaufbeuren.
Coupled synthetic peptides, representing the sequences of amino acids 130-160, 141-160 and 145-160 of foot-and-mouth disease virus O1K protein VP1, induced virus-binding and virus-neutralizing antibody response in guinea pigs, rabbits, and pigs. We also detected antibody response in guinea pigs after immunization with uncoupled peptides and in cattle with 21 aa-peptide-Keyhole-limpet hemocyanin (-KLH). The best results were obtained from 21 aa-peptide-KLH and 31 aa-peptide with or without KLH or thyroglobulin as carrier. Our preliminary results show the induction of virus-neutralizing antibodies to be obviously influenced by length of the peptide as well as by the kind of carrier and coupling.
Liebermann
H
H
Friedrich-Loeffler-Institut Insel Riems, Berlin.
Holl
U
U
Reimann
I
I
Nöckler
A
A
Schäfer
D
D
Thalmann
G
G
Dölling
R
R
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D005537:Aphthovirus / Q000276:immunology*; D002417:Cattle; D006168:Guinea Pigs; D007106:Immune Sera / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology*; D011817:Rabbits; D013552:Swine; D014614:Vaccines, Synthetic / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D007106:Immune Sera; D010446:Peptide Fragments; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines