title: Influence of immobilization on properties of polyphosphate glucokinase.
abstract: Polyphosphate glucokinase from Mycobacterium tuberculosis H37Ra was covalently bound to the glutaraldehyde-activated collagen coating silica gel, to the nylon, and to the CNBr-activated Sepharose. After immobilization kinetic properties of the enzyme were altered.
authors.affiliation: Department of Biochemistry and Drug Metabolism, Medical Academy, Lublin, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Kowalczyk
authors.forename: O
authors.initials: O
authors.lastname: Szymona
authors.forename: T
authors.initials: T
authors.lastname: Wolski
mesh_terms: D002846:Chromatography, Affinity; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009169:Mycobacterium tuberculosis / Q000201:enzymology; D010770:Phosphotransferases / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D004800:Enzymes, Immobilized; D010770:Phosphotransferases; C024812:polyphosphate-glucose phosphotransferase