Radiation-induced gene amplification in rodent and human cells.
Ionizing and UV radiations induce amplification of SV40 DNA sequences integrated in the genome of Chinese hamster cells and increase amplification of the dihydrofolate reductase (DHFR) gene during methotrexate selection in human skin fibroblasts of a patient with ataxia telangiectasia. By cell fusion experiments it could be shown that SV40 gene amplification is mediated by one or several diffusible trans-acting factors induced or activated in a dose dependent manner by all types of radiation. One of these factors binds to a 10 bp sequence within the minimal origin of replication of SV40. In vivo competition with an excess of a synthetic oligonucleotide comprising this sequence blocks radiation-induced amplification.
Lücke-Huhle
C
C
Nuclear Research Centre, Institute of Genetics and Toxicology, Karlsruhe, FRG.
Gloss
B
B
Herrlich
P
P
D000818:Animals; D001260:Ataxia Telangiectasia / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000235:genetics / Q000528:radiation effects; D004261:DNA Replication / Q000235:genetics / Q000528:radiation effects; D005784:Gene Amplification / Q000528:radiation effects*; D006801:Humans; D008969:Molecular Sequence Data; D013539:Simian virus 40 / Q000235:genetics; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics
D004247:DNA; D013762:Tetrahydrofolate Dehydrogenase