Identification of oral yeasts by polyacrylamide gel electrophoresis.
Protein profiles of sonicated cells for 9 species of yeast isolated from oral samples were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and visualized with a silver stain. The profiles obtained on 12% acrylamide gels were distinct, and characteristic for 6 species of Candida, and Torulopsis glabrata, Yarrowia lipolytica, and Saccharomyces cerevisiae. Using this method, 79 fresh isolates of yeasts from saliva samples were identified; 58 as Candida albicans, 9 as Candida parapsilosis, 1 as Candida tropicalis, and 11 as T. glabrata. SDS-PAGE offers a rapid, convenient alternative or adjunct to yeast identification systems based on carbohydrate assimilation tests.
Maiden
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MF
Forsyth Dental Center, Boston, Massachusetts.
Tanner
A
A
D000328:Adult; D002175:Candida / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D012440:Saccharomyces / Q000302:isolation & purification; D012463:Saliva / Q000382:microbiology*; D015003:Yeasts / Q000302:isolation & purification*