title: Transfer region of IncI1 plasmid R64 and role of shufflon in R64 transfer.
abstract: To locate the transfer region of the 122-kiloase plasmid R64drd-11 belonging to incompatibility group I1, a series of deletion derivatives was constructed by in vitro recombinant DNA techniques followed by double homologous recombination in vivo. A plasmid designated pKK609 and bearing a 56.7-kilobase R64 sequence was the smallest transferable plasmid. A plasmid designated pKK610 and no longer possessing the 44-base-pair sequence of the R64 transfer system is located at one end. The other end of the R64 transfer region comprises a DNA segment of about 19 kilobases responsible for pilus formation. Shufflon, DNA with a novel rearrangement in R64, was found to be involved in pilus formation.
authors.affiliation: Department of Biology, Tokyo Metropolitan University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Komano
authors.forename: N
authors.initials: N
authors.lastname: Funayama
authors.forename: S R
authors.initials: SR
authors.lastname: Kim
authors.forename: T
authors.initials: T
authors.lastname: Nisioka
mesh_terms: D002872:Chromosome Deletion; D003001:Cloning, Molecular; D003090:Coliphages; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D010861:Fimbriae, Bacterial; D009154:Mutation; D011815:R Factors / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D004251:DNA Transposable Elements