Identification of four different CD46 (MCP) molecules with anti-peptide antibodies.
Four protein isoforms of the human CD46 molecule (MCP) have been identified using rabbit antisera against synthetic peptides corresponding to predicted cytoplasmic carboxyl-terminal sequences. Two different cytoplasmic tails, CD46 CYT 1 and CD46 CYT 2, were detected in CD46 molecules isolated from almost every cell type examined. Bands of 56 and 66 kDa were obtained from SDS-PAGE analyses of various cell types with both CYT 1 and CYT 2 antisera, which indicated that the size polymorphism of the CD46 molecule is not due to variations in the cytoplasmic tail and that each species contained two different sized molecules.
Purcell
D F
DF
Austin Research Institute, Austin Hospital, Heidelberg, Victoria, Australia.
Jonstone
R W
RW
McKenzie
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IF
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D015703:Antigens, CD / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011817:Rabbits / Q000276:immunology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D015703:Antigens, CD; C497857:CD46 protein, human; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D010455:Peptides