title: Anti-murine IL-6 receptor antibody inhibits IL-6 effects in vivo.
abstract: Thrombopoiesis, as well as antibody production, is one of the major events in which interleukin-6 (IL-6) has been reported to be involved. Polyclonal anti-murine IL-6 receptor antibody was prepared to examine the effect of the antibody on these events in IL-6-treated mice. Administration of the anti-mIL-6R antibody inhibited the IL-6-induced increase in the number of platelets. Enhancement of the serum level of DNP-specific antibody by intraperitoneal injection of IL-6 was inhibited completely with simultaneous administration of the anti-mIL-6R antibody. The level of DNP-specific antibody was decreased, even below the basal value, by the higher dose of anti-mIL-6R antibody, indicating its effect also on endogenous IL-6. This work provides evidence that anti-IL-6R antibody inhibits IL-6 function in vivo, and provides an animal model of the therapeutic use of anti-IL-6R antibody for IL-6-related disease.
authors.affiliation: Biotechnology Research Laboratory, Tosoh Corporation, Kanagawa, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Yasukawa
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: M
authors.initials: M
authors.lastname: Anzai
authors.forename: R
authors.initials: R
authors.lastname: Goitsuka
authors.forename: A
authors.initials: A
authors.lastname: Hasegawa
authors.forename: Y
authors.initials: Y
authors.lastname: Ohsugi
authors.forename: T
authors.initials: T
authors.lastname: Taga
authors.forename: T
authors.initials: T
authors.lastname: Kishimoto
mesh_terms: D000818:Animals; D000906:Antibodies / Q000502:physiology; D001792:Blood Platelets / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004140:Dinitrophenols; D004195:Disease Models, Animal; D006168:Guinea Pigs; D006241:Haptens / Q000187:drug effects; D015850:Interleukin-6 / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010976:Platelet Count; D011971:Receptors, Immunologic / Q000276:immunology*; D019947:Receptors, Interleukin-6; D011994:Recombinant Proteins / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000187:drug effects; D014407:Tumor Cells, Cultured; D000093242:Interleukin-6 Inhibitors
chemical_list: D000906:Antibodies; D004140:Dinitrophenols; D006241:Haptens; D015850:Interleukin-6; D011971:Receptors, Immunologic; D019947:Receptors, Interleukin-6; D011994:Recombinant Proteins; D012710:Serum Albumin, Bovine; D000093242:Interleukin-6 Inhibitors; C034638:dinitrophenyl-bovine serum albumin