title: Cephalosporin C acylase in the autolysis of filamentous fungi.
abstract: Cephalosporin C acylase activity was studied using fluorescamine determination of free--NH2 groups produced in the deacylation of cephalosporin C by the enzyme. Fourteen fungi from different genera were studied and low extracellular cephalosporin C acylase activity was found in the genera Aspergillus, Fusarium and Penicillium. Forty one fungi of these genera were checked but not all presented acylase activity. The enzyme was generally found to be an extracellular enzyme and during the process of autolysis its activity increased with incubation time and with increasing pH of the medium. In no case was beta-lactamase activity detected. Penicillium rugulosum and Penicillium griseofulvum were identified as good cephalosporin C acylase producers. Deacetyl esterase activity was also detected in these fungi.
authors.affiliation: Departmento de Microbiología Aplicada, Centro de Investigaciones Biológicas (CSIC), Madrid, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Reyes
authors.forename: M J
authors.initials: MJ
authors.lastname: Martinez
authors.forename: C
authors.initials: C
authors.lastname: Alfonso
authors.forename: J L
authors.initials: JL
authors.lastname: Copa-Patino
authors.forename: J
authors.initials: J
authors.lastname: Soliveri
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism*; D001230:Aspergillus / Q000201:enzymology / Q000648:ultrastructure; D001329:Autolysis; D002511:Cephalosporins / Q000378:metabolism*; D003470:Culture Media; D005658:Fungi / Q000201:enzymology* / Q000648:ultrastructure; D005670:Fusarium / Q000201:enzymology / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D010399:Penicillin Amidase / Q000378:metabolism*; D010407:Penicillium / Q000201:enzymology / Q000648:ultrastructure
chemical_list: D002511:Cephalosporins; D003470:Culture Media; C025163:cephalosporin C; D000581:Amidohydrolases; D010399:Penicillin Amidase