Biochemical identification of B-F and B-G allelic variants of the chicken major histocompatibility complex.
Biochemical methods were used to analyse B-F and B-G antigens of the chicken major histocompatibility complex (MHC). In a panel of 12 inbred or partially inbred chicken lines the MHC haplotypes, originally defined by serological and histogenetical methods, were compared. Using monoclonal 18-6G2, allele-specific B-G patterns were obtained by immunoblotting. Comparison of B-G12 and B-G2 revealed a shared banding pattern, but additional products were detected for B-G12. The B-F products of B2 and B12 had identical IEF patterns. The identical B-F products and partially shared B-G products might explain the serological cross-reaction between these haplotypes. In addition, the IEF pattern of B-F21 appeared similar to B-F2 and B-F12, but the partial proteolysis map showed a clear difference. Although two B-F bands could be detected per haplotype, no evidence for the expression of more than one B-F locus was found. The biochemical methods enabled a precise definition of expressed MHC products and can be a useful tool for the identification of B-alleles in other chicken lines or outbred chickens for their MHC antigens.
Hepkema
B G
BG
Department of Immunology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
van der Poel
A
A
Grosfeld-Stulemeyer
M C
MC
Hensen
E J
EJ
D000483:Alleles; D000818:Animals; D002645:Chickens / Q000276:immunology*; D003429:Cross Reactions; D004912:Erythrocytes / Q000737:chemistry* / Q000276:immunology; D014644:Genetic Variation / Q000235:genetics; D006649:Histocompatibility Antigens / Q000737:chemistry / Q000235:genetics*; D015151:Immunoblotting; D007525:Isoelectric Focusing
D006649:Histocompatibility Antigens