title: Rapid evaluation of topoisomerase inhibitors: caffeine inhibition of topoisomerases in vivo.
abstract: Caffeine was found to inhibit both type I and type II topoisomerases in vivo as judged by its effects on replicating simian virus 40 (SV40) chromosomes. The study was facilitated by the use of a rapid filter assay for the detection and characterization of topoisomerase inhibitors. The assay, which requires neither purified enzymes nor substrates, was able to identify both antagonists and poisons of type I and type II topoisomerases.
authors.affiliation: Department of Radiology, Ohio State University, Columbus 43210.
authors.forename: C G
authors.initials: CG
authors.lastname: Shin
authors.forename: J M
authors.initials: JM
authors.lastname: Strayer
authors.forename: M A
authors.initials: MA
authors.lastname: Wani
authors.forename: R M
authors.initials: RM
authors.lastname: Snapka
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D002166:Camptothecin / Q000494:pharmacology; D005047:Etoposide / Q000494:pharmacology; D000882:Haplorhini; D013713:Teniposide / Q000494:pharmacology; D059004:Topoisomerase I Inhibitors; D059005:Topoisomerase II Inhibitors
chemical_list: D059004:Topoisomerase I Inhibitors; D059005:Topoisomerase II Inhibitors; D002110:Caffeine; D005047:Etoposide; D013713:Teniposide; D002166:Camptothecin