PGE1 compared to PGE2/PGF2 alpha ratio as a marker for seminal fluid contamination of urine in studies of renal prostaglandin biosynthesis.
Occasional and unpredictable seminal plasma leakage (SPL) into the urinary system severely limits the usefulness of primary prostaglandin (PG) excretion rates as markers of renal synthesis in male subjects. Although reports to this effect appeared first some 10 years ago, no specific study has been done to define the magnitude of the problem, while several investigators persisted in the use of primary PG excretion rates, disregarding SPL as a confounding factor. We conducted a systematic study with a group of 5 healthy adult subjects who collected 24-h urine with an average frequency of one collection every other week for a total of 20 weeks. 35% of the urine collected contained seminal plasma. It is imperative to be able to disqualify compromised urine specimens in biological studies. With this objective in mind, we developed a method to identify contaminated samples based on the presence of PGE1. This is a major prostanoid in seminal plasma but is normally absent in 'clear' urine.
Ferretti
A
A
Beltsville Human Nutrition Research Center, USDA, MD 20705.
Judd
J T
JT
D000328:Adult; D000527:Alprostadil / Q000096:biosynthesis / Q000652:urine; D015415:Biomarkers; D015237:Dinoprost / Q000096:biosynthesis / Q000652:urine; D015232:Dinoprostone / Q000096:biosynthesis / Q000652:urine; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011453:Prostaglandins / Q000096:biosynthesis / Q000652:urine*; D012661:Semen; D013048:Specimen Handling
D015415:Biomarkers; D011453:Prostaglandins; D015237:Dinoprost; D000527:Alprostadil; D015232:Dinoprostone