UDP-GalNAc:NeuAc alpha 2,3Gal beta-R (GalNAc to Gal) beta 1,4-N-acetylgalactosaminyltransferase responsible for the Sda specificity in human colon carcinoma CaCo-2 cell line.
The UDP-GalNAc:NeuAc alpha 2,3Gal beta-R (GalNAc to Gal) beta-1,4-N-acetylgalactosaminyltransferase (beta 1,4GalNAc-transferase) is the enzyme responsible for the addition of the immunodominant sugar of the Sda isto-blood group determinant. In humans the enzyme is mainly expressed in the large intestine. We screened nine human colorectal carcinoma cell lines (SW-948, SW-948 FL, SW-480, SW-48, SW-1417, COLO-205, LOVO, HT-29 and CaCo-2) in order to ascertain the occurrence of beta 1,4GalNAc-transferase. Only in CaCo-2 cells the glycosyltransferase was detected and the activity increased with the degree of enterocytic differentiation. Nevertheless, in highly differentiated CaCo-2 cells the activity was thirty times lower than that found in cells detached from normal colon mucosa. These results support the notion that the expression of beta 1,4GalNAc-transferase is a marker of the colonic cell maturation.
Malagolini
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Dipartimento di Patologia Sperimentale, Università di Bologna, Italy.
Dall'Olio
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F
Serafini-Cessi
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F
D001789:Blood Group Antigens; D002454:Cell Differentiation; D002460:Cell Line; D003106:Colon / Q000201:enzymology; D003110:Colonic Neoplasms / Q000201:enzymology*; D005700:Galactosyltransferases / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology; D007700:Kinetics; D017350:N-Acetylgalactosaminyltransferases; D013379:Substrate Specificity
D001789:Blood Group Antigens; D005700:Galactosyltransferases; D017350:N-Acetylgalactosaminyltransferases; C071413:beta-1,4-N-acetyl-galactosaminyl transferase 2