Minisatellite DNA profiles: rapid sample identification in linkage analysis.
Locus-specific minisatellite probes detect multiple alleles with heterozygosities of greater than 90% when hybridised to HinfI and AluI restriction digests of human DNA. We have hybridised 4 of these probes to a panel of DNAs digested with 6 of the restriction enzymes which commonly reveal di-allelic polymorphisms in the human genome. All 6 enzymes detected multiple resolvable alleles with at least 1 of the 4 minisatellite probes tested, thus providing a rapid and efficient means to check family relationships and paternity on filters already being used for linkage analysis.
Telenius
H
H
Section of Human Cancer Genetics, Institute of Cancer Research, Surrey, UK.
Clark
J
J
Marcus
E
E
Royle
N
N
Jeffreys
A J
AJ
Ponder
B A
BA
Mathew
C G
CG
D015139:Blotting, Southern; D004276:DNA, Satellite; D005060:Europe; D005554:Forensic Medicine / Q000379:methods; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length
D004276:DNA, Satellite; D005819:Genetic Markers