title: Mechanisms of spontaneous mutation in DNA repair-proficient Escherichia coli.
abstract: This paper describes the DNA sequence analysis of 729 independent spontaneous lacI- mutations. This total is comprised of 478 novel mutations and 251 previously described events, and therefore should allow a more comprehensive view of spontaneous mutation in Escherichia coli. The spectrum is dominated by a hotspot (71% of all events). Mutations at this site consist of related addition and deletion events involving a number of repetitive sequences. Here we discuss how the frequency and proportion of these events vary in different DNA repair-deficient genetic backgrounds. The distribution of non-hotspot events includes base substitutions (38%), deletions (35%), frameshifts (14%), duplications (4%) and insertion elements (4%). G:C----A:T events dominate among base substitutions, while G:C----C:G events are the least common; the remaining types of base substitution are equally represented. Among deletions, a significant number do not display repeated sequences at their endpoints (26/72). However, almost all multiply recovered events (15/17) possess repeated sequences capable of accounting for the deletion endpoints. Similarly, over half of all duplications recovered (5/7) display repeated endpoints. Single-base frameshifts are equally divided between A:T and G:C sites, in each case (-) 1 events occur 3-fold more frequently that (+) 1 events. A comparative analysis of each mutational class recovered to lacI- spectra available in a variety of DNA repair/metabolism-deficient strains is presented here in an attempt to assess possible contributions from chemical, physical and enzymic sources of damage.
authors.affiliation: Department of Biology, York University, Toronto, Ont., Canada.
authors.forename: J A
authors.initials: JA
authors.lastname: Halliday
authors.forename: B W
authors.initials: BW
authors.lastname: Glickman
mesh_terms: D001483:Base Sequence; D004260:DNA Repair; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D016368:Frameshift Mutation; D008969:Molecular Sequence Data; D009154:Mutation
chemical_list: D004269:DNA, Bacterial