Molecular characterization and immunogenicity of neutralization-sensitive Babesia bigemina merozoite surface proteins.
Monoclonal antibodies binding to the surface of live Mexico isolate Babesia bigemina merozoites have defined 4 parasite-encoded surface antigens (36, 45, 55, and 58 kDa) that are potential targets for immune-mediated neutralization of merozoites. In this study, we have characterized the post-translational modification, antigenic polymorphism, and immunogenicity of these 4 proteins. Monoclonal antibody immunoaffinity-purified 36- and 55-kDa polypeptides were identical in gel electrophoresis to immunoprecipitated radiolabeled proteins, while the purified 45-kDa protein consisted of 2 closely spaced polypeptides with relative molecular weights of 45 and 43 kDa. The 36-, 45-, and 55-kDa proteins were post-translationally modified by incorporation of [3H]glucosamine and [3H]myristic acid, suggesting they are integral membrane proteins secured by a phosphatidylinositol anchor. Cross-reactivity studies with monoclonal and monospecific polyclonal antibodies revealed marked antigenic polymorphism of these 3 glycoproteins among diverse geographic isolates. In contrast, none of the polypeptides bound by anti-p58 monoclonal antibody were glycosylated or myristilated. Both monoclonal and monospecific polyclonal antibodies recognizing p58 bound to similar molecular weight proteins in 4 additional isolates of B. bigemina from Mexico, Puerto Rico, St. Croix, and Kenya, suggesting widespread conservation of p58 immunogenic epitopes among geographic isolates. Calves immunized with immunoaffinity purified gp45, gp55, or p58 antigens were able to neutralize the infectivity of merozoites as indicated by significant reductions in the peak parasitemia after experimental challenge. Precise definition and appropriate presentation of neutralization sensitive epitopes on gp45, gp55, or p58 may enhance the merozoite neutralizing immune response in immunized cattle.
McElwain
T F
TF
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.
Perryman
L E
LE
Musoke
A J
AJ
McGuire
T C
TC
D000818:Animals; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D000953:Antigens, Protozoan / Q000302:isolation & purification; D000954:Antigens, Surface / Q000302:isolation & purification; D001403:Babesia / Q000254:growth & development / Q000276:immunology*; D001404:Babesiosis / Q000517:prevention & control; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control; D003429:Cross Reactions; D007114:Immunization; D008562:Membrane Glycoproteins / Q000276:immunology / Q000302:isolation & purification; D008970:Molecular Weight; D009500:Neutralization Tests; D011499:Protein Processing, Post-Translational; D015800:Protozoan Proteins / Q000276:immunology* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins