title: Cloning of industrial Saccharomyces 2-microns plasmid variants by in vivo site-specific recombination.
abstract: Southern analyses defined several industrial Saccharomyces yeast strains with extensive 2-microns DNA polymorphism. Variants included insertions and deletions up to several hundred base pairs. To facilitate the investigation of yeast plasmid evolution we developed a novel method of cloning 2-microns plasmids by taking advantage of 2-microns circle in vivo site-specific recombination and an SMRI gene as a dominant selectable marker. This method can be applied to other organisms for the isolation of plasmid variants and provides a new approach to in vivo plasmid construction.
authors.affiliation: Department of Biology, University of Saskatchewan, Saskatoon, Canada.
authors.forename: W
authors.initials: W
authors.lastname: Xiao
authors.forename: G H
authors.initials: GH
authors.lastname: Rank
mesh_terms: D003001:Cloning, Molecular; D010957:Plasmids; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic; D012440:Saccharomyces / Q000235:genetics*; D014170:Transformation, Genetic