Basal and drug-induced cAMP levels in cortical slices from the tottering mouse.
Basal and drug-induced levels of cAMP were determined in cortical slices from mice which were homozygous for the tottering (tg/tg) gene defect as well as from co-isogenic controls (+/+). Basal levels of cAMP were 77 +/- 16% higher in tg/tg slices compared to the controls. This difference was abolished by exposure of the slices to propranolol, a beta-adrenergic receptor antagonist. Both isoproterenol and veratridine stimulated cAMP formation, but only small differences were observed in the cAMP levels in tg/tg and +/+ slices after this treatment. Of the veratridine-dependent increase in cAMP, approximately 40% was blocked by propranolol treatment of slices from both strains. The results suggest that a higher level of endogenous norepinephrine release in tottering mice contributes to an elevation of basal cAMP levels.
Tehrani
M H
MH
Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030.
Barnes
E M
EM
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008818:Mice, Neurologic Mutants / Q000378:metabolism*; D011433:Propranolol / Q000494:pharmacology*; D012016:Reference Values; D014701:Veratridine / Q000494:pharmacology*
D014701:Veratridine; D011433:Propranolol; D000242:Cyclic AMP; D007545:Isoproterenol