Increased vitamin E intake restores fish-oil-induced suppressed blastogenesis of mitogen-stimulated T lymphocytes.
We sought to determine whether fish-oil supplementation would suppress blastogenesis in vitro of concanavalin A (ConA)-stimulated peripheral blood mononuclear cells (PBMCs) and, if so, whether it could be reversed with increased intake of vitamin E. Healthy males ate a controlled basal diet providing a total of 40% of energy from fat when fed in conjunction with 15 g/d of either placebo oil (PO) or fish-oil concentrate (FOC) fortified with 15 mg alpha-tocopherol/d for three periods. The subjects were supplemented with PO for 10 wk (PO), with FOC for 10 wk (FOC), and with FOC plus an additional 200 mg alpha-tocopherol/d for 8 wk (FOC+E). During FOC supplementation mitogenic responsiveness of PBMCs to ConA was suppressed, but this effect was reversed by concurrent supplementation with all-rac-alpha-tocopherol (FOC+E). There was a significant positive relationship (P less than 0.001) between plasma alpha-tocopherol concentrations and responsiveness of T lymphocytes to ConA.
Kramer
T R
TR
Vitamin and Mineral Laboratory, US Department of Agriculture, Beltsville, MD 20705.
Schoene
N
N
Douglass
L W
LW
Judd
J T
JT
Ballard-Barbash
R
R
Taylor
P R
PR
Bhagavan
H N
HN
Nair
P P
PP
D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000494:pharmacology; D005395:Fish Oils / Q000494:pharmacology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D012044:Regression Analysis; D013601:T-Lymphocytes / Q000187:drug effects*; D014810:Vitamin E / Q000097:blood / Q000494:pharmacology*
D005395:Fish Oils; D003208:Concanavalin A; D014810:Vitamin E; D015118:Eicosapentaenoic Acid