title: Bowes human melanoma cell line. An immunocytochemical study.
abstract: Bowes human melanoma cell line was investigated immunocytochemically to localize S-100 protein, HMB-45 and intermediate filament proteins. The majority of the cells showed strong positive staining with antibodies directed against S-100 protein, HMB-45 and vimentin filaments. Antibodies directed against the other cytoskeletal proteins failed to show any reactivity. These findings suggest that this transformed cell line does not dedifferentiate in culture and continues to express the specific antigens of human melanoma cells. Thus, Bowes cell line provides a useful model for studying the cellular biology and pathology of malignant melanoma.
authors.affiliation: Institute of Anatomy, Medical Faculty, RWTH Aachen, Federal Republic of Germany.
authors.forename: T
authors.initials: T
authors.lastname: Lombardi
authors.forename: M
authors.initials: M
authors.lastname: Castellucci
mesh_terms: D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis; D008545:Melanoma / Q000032:analysis*; D009363:Neoplasm Proteins / Q000032:analysis*; D009418:S100 Proteins / Q000032:analysis; D014407:Tumor Cells, Cultured
chemical_list: D007381:Intermediate Filament Proteins; D009363:Neoplasm Proteins; D009418:S100 Proteins