title: Effects of carbodiimide structure on the immobilization of enzymes.
abstract: A series of water-soluble disubstituted carbodiimides of different structure was tested for enzyme immobilization. In the experiments, a polyacrylamide-type bead polymer possessing carboxylic functional groups was used as support. The enzymes immobilized were aminoacylase (N-acylamino acid amidohydrolase; EC 3.5.1.14), arginase (L-arginine amidinohydrolase; EC 3.5.3.1), cyclodextrin glycosyltransferase (alpha-1,4-glucan 4-glycosyltransferase, cyclizing; EC 3.2.1.19), glucoamylase (1,4-alpha-D-glucan glycohydrolase, EC 3.2.1.3), and carboxypeptidase B (peptidyl-L-lysine [L-arginine] hydrolase; EC 3.4.17.2). It was found that the degree of immobilization strongly depended on the structure of carbodiimide used.
authors.affiliation: Reanal Factory of Laboratory Chemicals, Budapest, Hungary.
authors.forename: B
authors.initials: B
authors.lastname: Szajáni
authors.forename: P
authors.initials: P
authors.lastname: Südi
authors.forename: G
authors.initials: G
authors.lastname: Klamár
authors.forename: Z M
authors.initials: ZM
authors.lastname: Jászay
authors.forename: I
authors.initials: I
authors.lastname: Petneházy
authors.forename: L
authors.initials: L
authors.lastname: Töke
mesh_terms: D001709:Biotechnology; D002234:Carbodiimides / Q000737:chemistry*; D004800:Enzymes, Immobilized; D015394:Molecular Structure
chemical_list: D002234:Carbodiimides; D004800:Enzymes, Immobilized