Differential induction of transcription for glucocorticoid-responsive genes in cultured rat hepatocytes.
Dexamethasone rapidly stimulated transcription of the tyrosine aminotransferase and metallothionein-I genes--but not of the phosphoenolpyruvate carboxykinase gene--in rat hepatocytes cultured in serum-free medium. This differential response was not observed for cyclic AMP. The results suggest that the phosphoenolpyruvate carboxykinase gene--but not the tyrosine aminotransferase and metallothionein-I genes--requires a factor which is permissive for stimulation of transcription by the glucocorticoid receptor.
Nebes
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VL
Department of Microbiology, Biochemistry and Molecular Biology, University of Pittsburgh, PA 15261.
DeFranco
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D
Morris
S M
SM
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D005796:Genes / Q000187:drug effects*; D005809:Genes, Regulator; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008668:Metallothionein / Q000235:genetics*; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*; D014444:Tyrosine Transaminase / Q000235:genetics*
D012333:RNA, Messenger; D003907:Dexamethasone; D008668:Metallothionein; D000242:Cyclic AMP; D014444:Tyrosine Transaminase; D010729:Phosphoenolpyruvate Carboxykinase (GTP)