Metabolism of 2,4-dinitrotoluene by Salmonella typhimurium strains TA98, TA98NR and TA98/1,8-DNP6, and mutagenicity of the metabolites of 2,4-dinitrotoluene and related compounds to strains TA98 and TA100.
The products detected in the incubation of 2,4-dinitrotoluene (2,4-DNT) with Salmonella typhimurium strains TA98 and TA98/1,8-DNP6 were nitrosonitrotoluenes, hydroxylaminonitrotoluenes, aminonitrotoluenes and dimethyl dinitroazoxybenzene. The capacity of TA98NR to reduce 2,4-DNT was much lower than that of TA98 and TA98/1,8-DNP6. The bacterial products showed no mutagenic activity in the Ames assay using TA98 and TA100. These results indicate that the lack of mutagenic activity of 2,4-DNT is not due to low reductive metabolism of 2,4-DNT by the bacteria, but to the lack of mutagenic activity of the bacterial reductive products of 2,4-DNT, including dimethyl dinitroazoxybenzene.
Sayama
M
M
Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
Mori
M
M
Nakada
Y
Y
Kagamimori
S
S
Kozuka
H
H
D000115:Acetylesterase / Q000172:deficiency; D004136:Dinitrobenzenes / Q000378:metabolism* / Q000633:toxicity; D009153:Mutagens / Q000378:metabolism* / Q000633:toxicity; D009601:Nitroreductases / Q000172:deficiency; D010084:Oxidation-Reduction; D012486:Salmonella typhimurium / Q000187:drug effects / Q000378:metabolism*; D013045:Species Specificity
D004136:Dinitrobenzenes; D009153:Mutagens; C016403:2,4-dinitrotoluene; D009601:Nitroreductases; D000115:Acetylesterase