Amplification of DNA sequences of Epstein-Barr and human immunodeficiency viruses using DNA-polymerase from Thermus thermophilus.
Using thermophilic DNA-polymerase from Thermus thermophilus we have amplified by polymerase chain reaction (PCR) specific DNA sequences of Epstein-Barr virus (EBV) and human immunodeficiency virus (HIV). DNA-polymerase from Thermus thermophilus (molecular mass of 80-86 kDa) differs in its physico-chemical properties from DNA-polymerase from Thermus aquaticus (molecular mass of 62-68 kDa). To amplify the specific EBV DNA sequence, oligonucleotide primers for the virus replicon region (oriP region) were used. As a result of amplification, a specific 405-bp DNA fragment was produced.
Glukhov
A I
AI
Research Center of Molecular Diagnostics, USSR Ministry of Health, Moscow.
Gordeev
S A
SA
Vinogradov
S V
SV
Kiselev
V I
VI
Kramarov
V M
VM
Kiselev
O I
OI
Severin
E S
ES
D001483:Base Sequence; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006678:HIV / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013824:Thermus / Q000201:enzymology*
D015342:DNA Probes; D004279:DNA, Viral; D004259:DNA-Directed DNA Polymerase