[Isolation of isoforms and crystallization of endonucleases of Serratia marcescens].
Two isoforms of an extracellular endonuclease, nuclease Sm1 and nuclease Sm2, were isolated from the culture filtrate of Serratia marcescens strain B10 M1 by the ligand-exchange chromatography on iminodiacetate-agarose in Cu2(+)-form, and chromatography on phosphocellulose and DEAE-Toyopearl 650S. The pI for nucleases Sm1 and Sm2 were found to be 7.1 and 6.7, respectively. The amino acid analysis and N-terminal amino acid sequencing of the proteins showed a significant degree of homology between the enzymes. The secondary structure of nuclease Sm2 was calculated. Crystals of nuclease Sm2 were obtained with the space group P2(1)2(1)2(1), a 69.0; b 106.7; c 74.8 A.
Bannikova
G E
GE
Blagova
E V
EV
Varlamov
V P
VP
Morgunova
E Iu
EIu
Dement'ev
A A
AA
Shliapnikov
S V
SV
D000595:Amino Acid Sequence; D002852:Chromatography, Ion Exchange; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D004706:Endodeoxyribonucleases; D004720:Endonucleases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D004722:Endoribonucleases; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D012706:Serratia marcescens / Q000201:enzymology*
D007527:Isoenzymes; D004706:Endodeoxyribonucleases; D004720:Endonucleases; D004722:Endoribonucleases; C021835:Serratia marcescens nuclease
Razdelenie izoform i kristallizatsiia éndonukleazy Serratia marcescens.