Inhibition of the activation of Hageman factor (factor XII) by human vascular endothelial cell culture supernates.
The supernatant fluid (conditioned medium) of cultured human vascular endothelial cells inhibits activation of Hageman factor (factor XII), whether by ellagic acid, bovine brain sulfatides, or bismuth subgallate; inhibition appears to be a property of one or more proteins in the culture supernates. This phenomenon may contribute to maintaining the fluidity of circulating blood by inhibiting surface activation of the intrinsic pathway of coagulation.
Ratnoff
O D
OD
Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, OH 44106.
Everson
B
B
Embury
P
P
Ziats
N P
NP
Anderson
J M
JM
Emanuelson
M M
MM
Malemud
C J
CJ
D002478:Cells, Cultured; D003470:Culture Media; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D004789:Enzyme Activation; D005174:Factor XII / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D009942:Organometallic Compounds / Q000494:pharmacology; D012501:Saphenous Vein; D013433:Sulfoglycosphingolipids / Q000494:pharmacology; D014357:Trypsin / Q000378:metabolism / Q000494:pharmacology; D014471:Umbilical Veins
D003470:Culture Media; D009942:Organometallic Compounds; D013433:Sulfoglycosphingolipids; D005707:Gallic Acid; D005174:Factor XII; D014357:Trypsin; C002170:bismuth subgallate