A case of B-CLL expressing P-glycoprotein in peripheral leukocytes. Methodological considerations.
P-glycoprotein, the molecular marker of multidrug resistance, was investigated by means of the specific monoclonal antibody C219 in circulating lymphocytes obtained from a patient affected by B-CLL, who had undergone chemotherapy with chlorambucil and CVP. Dot-immunobinding and immunoblotting revealed the presence of P-glycoprotein in the cell lysate, but the immunoperoxidase method and indirect immunofluorescence carried out with a fluorescence microscope and a cytofluorimeter failed to detect the protein. Some methodologic problems are discussed.
Carulli
G
G
Servizio di Ematologia, Università, Pisa, Italy.
Petrini
M
M
Vaglini
F
F
Marini
A
A
Ambrogi
F
F
Grassi
B
B
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000368:Aged; D005260:Female; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood*; D007962:Leukocytes / Q000032:analysis*; D008562:Membrane Glycoproteins / Q000097:blood*; D009363:Neoplasm Proteins / Q000097:blood*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins