title: Acyltransferase activities of the high-molecular-mass essential penicillin-binding proteins.
abstract: The high-molecular-mass penicillin-binding proteins (HMM-PBPs), present in the cytoplasmic membranes of all eubacteria, are involved in important physiological events such as cell elongation, septation or shape determination. Up to now it has, however, been very difficult or impossible to study the catalytic properties of the HMM-PBPs in vitro. With simple substrates, we could demonstrate that several of these proteins could catalyse the hydrolysis of some thioesters or the transfer of their acyl moiety on the amino group of a suitable acceptor nucleophile. Many of the acyl-donor substrates were hippuric acid or benzoyl-D-alanine derivatives, and their spectroscopic properties enabled a direct monitoring of the enzymic reaction. In their presence, the binding of radioactive penicillin to the PBPs was also inhibited.
authors.affiliation: Centre d'Ingénierie des Protéines, Université de Liège, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Adam
authors.forename: C
authors.initials: C
authors.lastname: Damblon
authors.forename: M
authors.initials: M
authors.lastname: Jamin
authors.forename: W
authors.initials: W
authors.lastname: Zorzi
authors.forename: V
authors.initials: V
authors.lastname: Dusart
authors.forename: M
authors.initials: M
authors.lastname: Galleni
authors.forename: A
authors.initials: A
authors.lastname: el Kharroubi
authors.forename: G
authors.initials: G
authors.lastname: Piras
authors.forename: B G
authors.initials: BG
authors.lastname: Spratt
authors.forename: W
authors.initials: W
authors.lastname: Keck
mesh_terms: D000217:Acyltransferases / Q000378:metabolism*; D000613:Aminobutyrates / Q000378:metabolism; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000378:metabolism*; D002384:Catalysis; D002462:Cell Membrane / Q000737:chemistry; D016983:Enterococcus / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D004952:Esters / Q000378:metabolism / Q000494:pharmacology; D006602:Hexosyltransferases; D006626:Hippurates / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D008970:Molecular Weight; D002267:Muramoylpentapeptide Carboxypeptidase / Q000378:metabolism*; D010400:Penicillin G / Q000378:metabolism; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D013302:Streptomyces / Q000737:chemistry; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000613:Aminobutyrates; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004952:Esters; D006626:Hippurates; D046915:Penicillin-Binding Proteins; D013438:Sulfhydryl Compounds; C014328:2-amino-4-phenylbutyric acid; D000217:Acyltransferases; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D010400:Penicillin G; C030514:hippuric acid