Enzyme immunoassay of somatostatin (SS)-like immunoreactive substance in bovine milk.
A sensitive and specific enzyme immunoassay (EIA) for somatostatin (SS)-like immunoreactivity (SS-LI) was developed with the use of beta-D-galactosidase labeled antigen. The minimum amount of SS-like immunoreactive substance (SS-IS) detectable by this method was 1.0 fmol/well (25 pmol/l). The level of SS-IS in bovine foremilk was about 20 pmol/l, and the level was unchanged after delivery. On the other hand, the levels of gastrin releasing peptide (GRP)-IS and vasoactive intestinal polypeptide (VIP)-IS in bovine foremilk were very high, but fell during 1 week after delivery to about 10% of those in foremilk.
Takeyama
M
M
Department of Hospital Pharmacy, Medical College of Oita, Japan.
Yanaga
N
N
Yarimizu
K
K
Ono
J
J
Takaki
R
R
Fujii
N
N
Yajima
H
H
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D007124:Immunoenzyme Techniques; D008892:Milk / Q000032:analysis*; D013004:Somatostatin / Q000032:analysis*
D013004:Somatostatin