Evidence for an age-correlated change in glutathione metabolism enzyme activities in human hair follicle.
In this investigation, glutathione peroxidase (GSH-PX), glutathione reductase (GSSG-RD), glutathione-S-transferase (GSH-S-T), gamma-glutamyl transpeptidase (gamma-GT) and glucose-6-phosphate dehydrogenase (G6PDH) were measured in human hair follicle obtained by plucking as source of keratinized cells. This non-invasive method was used on 27 men and women volunteers ranging from 19 to 102 years. Our results show that GSSG-RD, GSH-S-T, gamma-GT and G6PDH activities decrease as a function of age, whereas GSH-PX activity does not vary. We discriminated 2 groups: a first one from 19 to 60 years with a large dispersion of the enzymatic activities and a second one corresponding to elderly people (up to 70) with a smaller dispersion of the values. This study suggests the keratinocytes possess an age-correlated enzymatic detoxification response potential.
Kermici
M
M
L'OREAL Research Laboratory, Aulnay sous bois, France.
Pruche
F
F
Roguet
R
R
Prunieras
M
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006197:Hair / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D005954:Glucosephosphate Dehydrogenase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005723:gamma-Glutamyltransferase; D005982:Glutathione Transferase; D005978:Glutathione