Salmonellae of serotypes gallinarum and pullorum grouped by biotyping and fimbrial-gene probing.
When salmonellae of serotypes Gallinarum (50 isolates) and Pullorum (36 isolates), that produce non-adhesive (type-2) fimbriae, were tested for their reactions in biochemical tests, 81 (94%) were found to belong to three distinct biochemical groups, I-III. Interaction of HinfI-digested DNA of both Gallinarum and Pullorum with a probe of accessory genes of type-1 fimbriation in serotype Typhimurium gave one type of Southern hybridisation pattern that was readily distinguished from that of Typhimurium strains. With a probe of the Typhimurium fimbrial subunit gene, Pullorum isolates were separated into strongly and weakly probe-reactive groups which showed restriction fragment-length polymorphism; these latter groups corresponded to biochemical groups II and III, respectively.
Crichton
P B
PB
Department of Medical Microbiology, University of Dundee Medical School, Ninewells Hospital.
Old
D C
DC
D015373:Bacterial Typing Techniques; D005075:Biological Evolution; D015139:Blotting, Southern; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D005285:Fermentation; D010861:Fimbriae, Bacterial / Q000032:analysis; D012150:Polymorphism, Restriction Fragment Length; D012475:Salmonella / Q000145:classification* / Q000378:metabolism
D015342:DNA Probes; D004269:DNA, Bacterial