title: Leucocyte function in rats with naturally occurring gingivitis.
abstract: The severity of gingivitis in relation to abnormal macrophage chemotaxis and superoxide (O2-) production was investigated in rats with naturally occurring gingivitis (plaque-susceptible rats). Macrophage chemotaxis was measured by the membrane filter method; zymosan-activated serum was used as a chemo-attractant. O2- production was measured by the reduction of cytochrome c method. Opsonized zymosan, phorbol myristate acetate and calcium ionophore A23187 were used as stimulants. The macrophage chemotaxis and O2- production in the plaque-susceptible rats with deep pockets were significantly lower than those in the susceptible rats with shallow pockets or the normal group. In addition, there was a negative correlation between the severity of gingivitis in plaque-susceptible rats and macrophage chemotaxis or O2- production. These findings suggest that impairement of the host defence mechanisms due to the reduction in macrophage chemotaxis and O2- production is related to the progression of periodontal disease in plaque-susceptible rats.
authors.affiliation: Department of Pharmacology, Osaka Dental University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ohura
authors.forename: M
authors.initials: M
authors.lastname: Shinohara
authors.forename: K
authors.initials: K
authors.lastname: Ogata
authors.forename: A
authors.initials: A
authors.lastname: Nishiyama
authors.forename: M
authors.initials: M
authors.lastname: Mori
mesh_terms: D000818:Animals; D002464:Cell Migration Inhibition; D002633:Chemotaxis / Q000502:physiology*; D003773:Dental Plaque; D004198:Disease Susceptibility; D005891:Gingivitis / Q000473:pathology*; D008264:Macrophages / Q000378:metabolism / Q000502:physiology*; D010514:Periodontal Pocket / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D013481:Superoxides / Q000378:metabolism*
chemical_list: D013481:Superoxides