[Production of proteinases in the process of developing cultures of Streptomyces thermovulgaris T-54].
The secretion of some proteolytic enzymes by Streptomyces thermovulgaris T 54 was studied using artificial chromogenic substrates of proteinases. Maximum accumulation of the enzymes hydrolysing Z-Glu-pNA and Z-Ala-Ala-Leu-pNA occurred during autolysis of the culture. Two peaks of activity were observed, when DNP-Gly-Gly-Ile-Arg was used as a substrate, one of them being correspondent to prevalence of dense colonies in the culture and the other to the prevalence of friable networks of hyphae.
Khaĭdarova
N V
NV
Rudenskaia
G N
GN
Stepanov
V M
VM
Egorob
N S
NS
D000595:Amino Acid Sequence; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000096:biosynthesis*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D013302:Streptomyces / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D013379:Substrate Specificity
D003470:Culture Media; D010450:Endopeptidases
Produktsiia proteinaz v protsesse razvitiia kul'turury Streptomyces thermovulgaris T-54.