Microcystin-LR, a potent protein phosphatase inhibitor, prolongs the serotonin- and cAMP-induced currents in sensory neurons of Aplysia californica.
Microcystin-LR (MCYST-LR), a hepatotoxin produced by cyanobacteria, inhibited purified protein phosphatases (PrPs) from rabbit skeletal muscle and the enzymes from Aplysia with an IC50 of approximately 10(-10) M. MCYST-LR also prolonged both serotonin- (5-HT) and cyclic adenosine monophosphate-induced inward currents in sensory neurons of Aplysia. These results, which are consistent with inhibition of Aplysia PrPs, indicate that MCYST-LR may be a useful probe to elucidate the function of PrPs in neural tissues.
Ichinose
M
M
Department of Neurobiology and Anatomy, University of Texas Medical School, Houston 77225.
Endo
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Critz
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Shenolikar
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Byrne
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D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D001048:Aplysia / Q000187:drug effects*; D000242:Cyclic AMP / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D008387:Marine Toxins; D008564:Membrane Potentials / Q000187:drug effects; D052998:Microcystins; D009475:Neurons, Afferent / Q000187:drug effects*; D010456:Peptides, Cyclic / Q000494:pharmacology*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D012701:Serotonin / Q000502:physiology*
D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; D015124:8-Bromo Cyclic Adenosine Monophosphate; D012701:Serotonin; D000242:Cyclic AMP; D010749:Phosphoprotein Phosphatases; C057862:cyanoginosin LR