title: Comparison of the appropriateness of the latex and Elisa plasma D-dimer determination for the diagnosis of deep venous thrombosis.
abstract: Thirty-two patients suspected of deep venous thrombosis (DVT) of the leg were evaluated after simple plasma tests (latex D-dimer and Elisa D-dimer) against echography and phlebography as the gold standard of DVT. Seven patients showed negative results in the latter test. The classification conformity between the D-dimer methods amounted 84.4%. With regard to the diagnostic classification a high specificity of 100% was found for the latex D-dimer test at the cut-off level of 450 ng/ml, whereas for sensitivity, 96% was reached. The Elisa D-dimer test was striking by its high sensitivity of 100%, but its low specificity of 28.5%. In summary, these results demonstrate that when there is a suspicion of DVT according to the clinical symptoms, a negative latex test may reject the diagnosis, whereas the latex test is less useful to establish the diagnosis. On the contrary, the Elisa D-dimer test has a too high false-positive rate. The latex D-dimer test seems to be a reliable tool to exclude the diagnosis after which an ascending contrast venography can be omitted.
authors.affiliation: Department of Internal Medicine, De Wever Hospital, Heerlen, The Netherlands.
authors.forename: G S
authors.initials: GS
authors.lastname: Chang-Liem
authors.forename: F A
authors.initials: FA
authors.lastname: Lustermans
authors.forename: J W
authors.initials: JW
authors.lastname: van Wersch
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000933:Antifibrinolytic Agents / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism*; D006403:Hematologic Tests; D006801:Humans; D007840:Latex; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013924:Thrombophlebitis / Q000175:diagnosis*
chemical_list: D000933:Antifibrinolytic Agents; D005338:Fibrin Fibrinogen Degradation Products; D007840:Latex; C036309:fibrin fragment D