[Cloning of genes for 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase from methylotrophic Pseudomonas in Escherichia coli].
The genes of facultative methylotrophic bacteria Pseudomonas sp. M encoding tyrosine-sensitive and tryptophan-sensitive isoenzymes of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase were cloned separately on the pBR322 plasmid by complementation in Escherichia coli. Transcription of both genes in the new host was performed via the control of genes' own promoters. Cloned genes which are repressed by tyrosine and phenylalanine in their native host were expressed constitutively in E. coli.
Olekhnovich
I N
IN
Fomichev
Iu K
IuK
D010723:3-Deoxy-7-Phosphoheptulonate Synthase / Q000235:genetics*; D000446:Aldehyde-Lyases / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D010957:Plasmids; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics; D015183:Restriction Mapping
D010723:3-Deoxy-7-Phosphoheptulonate Synthase; D000446:Aldehyde-Lyases
Klonirovanie genov 3-dezoksi-D-arabinogeptulozonat-7-fosfat-sintazy metilotrofnykh Pseudomonas v kletkakh Escherichia coli.