Androgen-regulation of ornithine decarboxylase and S-adenosylmethionine decarboxylase genes.
Ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) are two key enzymes in polyamine biosynthesis. Both the ODC and the AdoMetDC gene is regulated by androgens in accessory sex organs of mice and rats, whereas only the ODC gene is androgen-responsive in rodent kidney. Androgenic responses in murine and rat kidneys are, however, dissimilar in that the induction of ODC activity and ODC mRNA accumulation is transient in the rat but sustained in the murine renal cells. In addition, in situ hybridization experiments with single-stranded cRNA probes revealed that ODC gene expression occurs in different subpopulations of epithelial cells of the proximal tubules in mice and rats. ODC and AdoMetDC genes are androgen-regulated in the same cell types of the accessory sex organs, as judged by hybridization histochemistry. Sequencing of the promotor region of the murine ODC gene has indicated the presence of several DNA elements for binding of transcription factors/regulatory proteins, including a putative androgen-response element at about 900 nucleotides upstream of the transcription start site.
Jänne
O A
OA
Rockefeller University, New York, New York 10021.
Crozat
A
A
Palvimo
J
J
Eisenberg
L M
LM
D012437:Adenosylmethionine Decarboxylase / Q000235:genetics*; D000728:Androgens / Q000502:physiology*; D000818:Animals; D015971:Gene Expression Regulation, Enzymologic; D007668:Kidney / Q000201:enzymology; D051379:Mice; D009955:Ornithine Decarboxylase / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats
D000728:Androgens; D012333:RNA, Messenger; D009955:Ornithine Decarboxylase; D012437:Adenosylmethionine Decarboxylase