title: [Usefulness of immunoenzyme diagnostic kits and latex tests for isolation of rotaviruses].
abstract: The aim of this study was to compare characteristic parameters of 4 diagnostic kits available in Poland-immunoenzymatic Rotazyme II and Enzygnost kits and latex kits Rotalex and Slidex. Studies were performed on 67 samples of feces of children treated because of diarrhea. The sensitivity, specificity, frequency of positive tests, false positives and false negatives and the accuracy of the tests under evaluation were determined. The results obtained were further verified using a reference test electrophoresis of RNA of rotavirus. The highest sensitivity was found for Enzygnost, Rotazyme II and Rotalex, 97%, 92% and 90%, respectively, and the lowest for Slidex- 79%, while the specificity was higher for latex kits than for immunoenzymatic kits. The accuracy of the results was highest for Rotalex kit (92%), next for Enzygnost kit (88%), Rotazyme (87%), and Slidex (84%). The significant correlation between OD value readings in spectrophotometer in Rotazyme II kit and the results of visual reading in latex test was found. All tested kits were found to be useful for diagnostic purposes. Rotalex kit due to the high accuracy of the results obtained, methodological simplicity, short time of testing and relatively low price could be a based test in hospital laboratories.
authors.affiliation: Zakład Wirusologii PZH w Warszawie.
authors.forename: Z
authors.initials: Z
authors.lastname: Jarzabek
authors.forename: W
authors.initials: W
authors.lastname: Gut
authors.forename: Z
authors.initials: Z
authors.lastname: Szczepaniak
authors.forename: A
authors.initials: A
authors.lastname: Panfist
mesh_terms: D000293:Adolescent; D002648:Child; D003951:Diagnostic Errors; D005060:Europe; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D007841:Latex Fixation Tests / Q000379:methods; D011933:Reagent Kits, Diagnostic / Q000592:standards; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000382:microbiology; D014481:United States
chemical_list: D011933:Reagent Kits, Diagnostic
vernacular_title: Ocena przydatności immunoenzymatycznych zestawów diagnostycznych i testów lateksowych do wykrywania rotawirusów.