title: Expression of polysialylated N-CAM during rat heart development.
abstract: Developmental patterns of immunoreactivity for the neural cell adhesion molecule (N-CAM) and alpha 2.8-linked polysialic acid (PSA) were identified in embryonic and postnatal rat heart by immunocytochemistry and immunoblotting. Polyclonal antibodies against N-CAM and a monoclonal antibody which recognises only polymers of PSA with a chain length greater than eight units were used. Gold- and alkaline-phosphatase-labelled antibodies were used for detection. The N-CAM polypeptide isoform pattern seen by immunoblotting after endoneuraminidase treatment changed as development progressed. During embryonic development a 160-kDa polypeptide isoform was predominant. Around birth, 130-, 160- and 170-kDa polypeptide isoforms were found. The expression of the 130- and 170-kDa isoforms diminished until finally, in the adult, weak immunoreactivity for bands of 120-, 130- and 160-kDa was seen. In general the extent and intensity of PSA and N-CAM immunostaining in rat heart increased until birth and declined thereafter. Early in development prominent immunostaining for PSA and N-CAM was seen in the epicardium while later in development this area was only weakly stained. Initially myocardial cells, endocardial cells and some cells in the atrioventricular cushions were immunoreactive for both PSA and N-CAM. Later in development N-CAM immunostaining was more prominent than PSA immunoreactivity, reflecting a decrease in N-CAM polysialylation, which was also seen by immunoblotting. During innervation of the heart, nerve fibres were strongly immunostained for PSA and N-CAM, and this was the only immunostaining seen in adult heart.
authors.affiliation: Department of Cell and Molecular Pathology, University of Zürich Medical School, Switzerland.
authors.forename: P M
authors.initials: PM
authors.lastname: Lackie
authors.forename: C
authors.initials: C
authors.lastname: Zuber
authors.forename: J
authors.initials: J
authors.lastname: Roth
mesh_terms: D000818:Animals; D001283:Atrioventricular Node / Q000737:chemistry; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006046:Gold; D006321:Heart / Q000196:embryology* / Q000254:growth & development / Q000294:innervation; D007150:Immunohistochemistry; D009206:Myocardium / Q000737:chemistry; D011134:Polysaccharides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012794:Sialic Acids / Q000378:metabolism*
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D011134:Polysaccharides; D012794:Sialic Acids; C021319:polysialic acid; D006046:Gold