[Establishment and characterization of a new gastric cancer cell line (OCUM-1), derived from Borrmann type IV tumor].
A new human gastric cancer cell line (OCUM-1), which was derived from Borrmann type IV tumor of the stomach, was established. The cell line grew sometimes singly and sometimes in clusters, and continued to grow for more than 3 years. It's doubling time was 33.2 hours, chromosomal mode was 50, and nuclear DNA ploidy pattern was diploid. The cells could grow in nude mice. It produced carcinoembryonic antigen, carbohydrate antigen 19-9, and cancer-associated antigen SPan-1 and expressed epidermal growth factor receptor. Supplementation of epidermal growth factor to culture medium increased the cell number statistically significantly. It was decreased by supplementation of chondroitin sulphate. So the cell line OCUM-1 might be useful for the study of gastric cancer, especially Borrmann type IV gastric cancer.
Kubo
T
T
First Department of Surgery, Osaka City University Medical School, Japan.
D000328:Adult; D000818:Animals; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured