title: The formation of 3 alpha- and 3 beta-acetoxytropanes by Datura stramonium transformed root cultures involves two acetyl-CoA-dependent acyltransferases.
abstract: Tropine (tropan-3 alpha-ol) is an intermediate in the formation of hyoscyamine. An acyltransferase activity that can acetylate tropine using acetylcoenzyme A as cosubstrate has been found in transformed root cultures of Datura stramonium. A further acyltransferase activity that acetylates pseudotropine (tropan-3 beta-ol) with acetyl-coenzyme A is also present. These two activities can be partially resolved by anion-exchange chromatography, some fractions containing only the pseudotropine-utilizing activity. The basic properties of these two enzymes are reported and their roles in forming the observed alkaloid spectrum of D. stramonium roots discussed.
authors.affiliation: Genetics and Microbiology Department, AFRC Institute of Food Research, Norwich Laboratory, UK.
authors.forename: R J
authors.initials: RJ
authors.lastname: Robins
authors.forename: P
authors.initials: P
authors.lastname: Bachmann
authors.forename: T
authors.initials: T
authors.lastname: Robinson
authors.forename: M J
authors.initials: MJ
authors.lastname: Rhodes
authors.forename: Y
authors.initials: Y
authors.lastname: Yamada
mesh_terms: D000123:Acetyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D001285:Atropine / Q000096:biosynthesis; D002852:Chromatography, Ion Exchange; D013286:Datura stramonium / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010946:Plants, Medicinal; D010947:Plants, Toxic; D014326:Tropanes / Q000378:metabolism*
chemical_list: D014326:Tropanes; C071887:3-acetyltropine; D001285:Atropine; C005864:tropine; D000123:Acetyltransferases; C071889:acetyl CoA-tropine acetyltransferase; C071888:acetyl-CoA-pseudotropine acetyltransferase