title: Multihormonal regulation of the human prolactin gene expression from 5000 bp of its upstream sequence.
abstract: We have cloned DNA sequences extending up to 6000 bp upstream from the first exon of the human prolactin (hPRL) gene. 5000 bp of these upstream sequences were fused to a CAT reporter gene and shown to provide tissue-specific transient expression in rat pituitary GH3 cells. Multihormonal response was found in this transient expression assay, leading to significant 2- to 5-fold induction by addition of 8-chlorophenylthio-cyclic AMP, thyrotropin-releasing hormone, epidermal growth factor, basic fibroblast growth factor, phorbol myristate acetate, a calcium channel agonist (Bay K-8644) and triiodothyronine. A 3-fold inhibition was observed in the presence of the glucocorticoid agonist dexamethasone. The sequence of the hPRL promoter was determined up to coordinate -3470. Computer similarity search between the rat and human sequences showed two highly conserved regions corresponding to the proximal and distal tissue specific enhancers described in both PRL promoters.
authors.affiliation: Laboratoire de Biologie Moléculaire et de Génie Génétique, Université de Liège, Sart Tilman, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Berwaer
authors.forename: P
authors.initials: P
authors.lastname: Monget
authors.forename: B
authors.initials: B
authors.lastname: Peers
authors.forename: M
authors.initials: M
authors.lastname: Mathy-Hartert
authors.forename: E
authors.initials: E
authors.lastname: Bellefroid
authors.forename: J R
authors.initials: JR
authors.lastname: Davis
authors.forename: A
authors.initials: A
authors.lastname: Belayew
authors.forename: J A
authors.initials: JA
authors.lastname: Martial
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D016386:Chromosome Walking; D003001:Cloning, Molecular; D005091:Exons; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D011388:Prolactin / Q000235:genetics*; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D011388:Prolactin