title: Pathogenicity and antigenic components of excysted Giardia lamblia isolated from patients in Riyadh, Saudi Arabia.
abstract: Giardia lamblia cysts were isolated from patients in Riyadh, Saudi Arabia. Cysts and trophozoites (from axenically excysted cysts) were given orally by gavage to mice to establish the pathogenicity of the Riyadh isolate. There was no effect of varying the dose of administered parasite on parasite excretion or morbidity. A typical pathologic pattern of giardiasis was demonstrated by histologic methods and electron microscopy. Antigenic components of the Riyadh isolate were compared with the Portland strain and with Entamoeba histolytica by gel diffusion immunoprecipitation and immunoblotting. There were few antigens in common between Riyadh isolate and the Portland strain, and little cross-reactivity of the Riyadh isolate with Entamoeba histolytica was observed.
authors.affiliation: Security Forces Hospital, Riyadh, Saudi Arabia.
authors.forename: M H
authors.initials: MH
authors.lastname: al-Tukhi
authors.forename: M N
authors.initials: MN
authors.lastname: al-Ahdal
authors.forename: S R
authors.initials: SR
authors.lastname: Das
authors.forename: S
authors.initials: S
authors.lastname: Sadiqi
authors.forename: Y
authors.initials: Y
authors.lastname: Siddiqui
authors.forename: J
authors.initials: J
authors.lastname: Ackers
authors.forename: W
authors.initials: W
authors.lastname: Peters
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D003429:Cross Reactions; D004748:Entamoeba histolytica / Q000276:immunology; D016829:Giardia lamblia / Q000276:immunology / Q000472:pathogenicity* / Q000648:ultrastructure; D005873:Giardiasis / Q000469:parasitology* / Q000473:pathology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D005779:Immunodiffusion; D007421:Intestine, Small / Q000469:parasitology / Q000473:pathology / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000469:parasitology / Q000648:ultrastructure; D012529:Saudi Arabia
chemical_list: D000953:Antigens, Protozoan; D007106:Immune Sera