Plasmid content of piliated and nonpiliated forms of Moraxella bovis.
Plasmid profiles were compared between nonpiliated and piliated forms of Moraxella bovis isolates. The piliated form of M bovis isolate IBH64 contained 1 fewer plasmid than did the nonpiliated form. Piliated and nonpiliated cells of IBH64 contained plasmids having molecular size of 45, 32.8, 4.9, and 4.6 kilobases (kb). Single- and double-restriction endonuclease digestion by Ava I and Nde I indicated that the size of the additional plasmid carried by the nonpiliated form of IBH64 was approximately 43.6 kb. The M bovis isolates, Newport and GRS, contained the same number of plasmids in either their piliated or nonpiliated form.
Wilt
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Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-5519.
Wu
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Bird
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Toivio-Kinnucan
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D004262:DNA Restriction Enzymes; D010861:Fimbriae, Bacterial / Q000032:analysis*; D008854:Microscopy, Electron; D008967:Molecular Biology; D009016:Moraxella / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure*; D010957:Plasmids / Q000235:genetics*
D004262:DNA Restriction Enzymes