Blood group antigen expression in developing pancreas and in induced pancreatic cancer cells in Syrian hamsters.
Expression of blood group-related antigens and the binding pattern of Ulex europaeus-I (UEA-I) in fetal and newborn hamster pancreases was examined immunohistochemically by monoclonal antibodies (MoAbs) against blood group antigens and by peroxidase-conjugated UEA-I. The fetal hamster pancreas was detected histologically at the 12th day of gestation. MoAbs A, B and Le(a) were not immunoreactive with pancreatic cells in any stage of development nor were they after birth. Le(x) was first expressed in the luminal surface of duct cells at the 13th day of gestation, disappeared at the 15th day of gestation, was re-expressed after birth in acinar cells and was absent in the adult pancreas. Le(b) and Le(y) were expressed in acinar cells in late fetal and early newborn stages, but disappeared with maturation and were absent in the adult pancreas. UEA-I binding, however, was demonstrated in both fetal and adult pancreatic tissue. In cancer cells induced by BOP, blood group antigens except for Le(a) and UEA-I binding were found in various reactivity. These findings suggest that in hamsters (i) A and B antigens are tumor-related antigens; (ii) H, Le(b), Le(x) and Le(y) are oncofetal antigens; and (iii) fucosylation is an important event in cell differentiation.
Takiyama
Y
Y
Eppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha 68105.
Egami
H
H
Pour
P M
PM
D000017:ABO Blood-Group System; D000818:Animals; D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D006224:Cricetinae; D005260:Female; D006017:Glycolipids / Q000032:analysis; D007124:Immunoenzyme Techniques; D007519:Isoantigens; D037102:Lectins / Q000378:metabolism; D016256:Lewis X Antigen; D008297:Male; D008647:Mesocricetus; D010179:Pancreas / Q000166:cytology / Q000196:embryology / Q000254:growth & development / Q000276:immunology*; D010190:Pancreatic Neoplasms / Q000139:chemically induced / Q000276:immunology*; D037121:Plant Lectins
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D006017:Glycolipids; D007519:Isoantigens; D037102:Lectins; D016256:Lewis X Antigen; D037121:Plant Lectins; C036850:Ulex europaeus lectins