title: Shared actions of endotoxin and taxol on TNF receptors and TNF release.
abstract: Bacterial lipopolysaccharide (LPS) exerts profound effects on mammalian hosts in part by inducing macrophages to release tumor necrosis factor-alpha (TNF-alpha); the mechanisms involved are unresolved. The microtubule stabilizer taxol shared two actions of LPS on macrophages: it rapidly decreased TNF-alpha receptors and triggered TNF-alpha release. Both actions of taxol were absent in LPS-hyporesponsive C3H/HeJ mice. In recombinant inbred mice, the genes controlling responses to LPS and to taxol were closely linked. Dexamethasone blocked release of TNF-alpha by both stimuli but did not block the decrease in TNF-alpha receptors. Thus, a protein associated with microtubules may be a cellular target of LPS.
authors.affiliation: Department of Medicine, Cornell University Medical College, New York, NY 10021.
authors.forename: A H
authors.initials: AH
authors.lastname: Ding
authors.forename: F
authors.initials: F
authors.lastname: Porteu
authors.forename: E
authors.initials: E
authors.lastname: Sanchez
authors.forename: C F
authors.initials: CF
authors.lastname: Nathan
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D003433:Crosses, Genetic; D003907:Dexamethasone / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D017239:Paclitaxel; D011956:Receptors, Cell Surface / Q000187:drug effects* / Q000378:metabolism; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D000470:Alkaloids; D008070:Lipopolysaccharides; D011956:Receptors, Cell Surface; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha; D003907:Dexamethasone; D017239:Paclitaxel