DNA ploidy and proliferative activity of human pulmonary epithelium.
DNA ploidy and distribution has been determined in normal and abnormal bronchial, bronchiolar and alveolar epithelium from 22 patients, aged between 0 and 85 years, 9 of whom had received chemotherapy for malignant disease. The DNA ploidy was diploid in all the specimens examined. The S + G2/M fraction was significantly greater in diseased than normal bronchial trees. In the bronchial epithelium, mean values +/- the standard deviation (SD) were 5.5 +/- 2.2% vs 1.1 +/- 0.6%, in bronchiolar epithelium 4.6 +/- 1.6% vs 1.0 +/- 0.9% and in alveolar epithelium 4.6 +/- 1.6% vs 0.8 +/- 0.5%. The highest S + G2/M value of 8.9% was obtained from inflamed bronchial epithelium. Polyploid cells up to the octaploid range occurred infrequently but their incidence was slightly increased to between 0.16% and 0.9% in diseased lungs and in patients who had received chemotherapeutic drugs. It was concluded that (1) non-cancerous drugs. It was concluded that (1) non-cancerous pulmonary epithelium is diploid, that (2) pulmonary epithelium shows steady-state renewal at all ages and polyploid cells are rare under normal conditions and that (3) the S + G2/M fraction increases up to approximately 10% in reactive proliferative states.
Hamada
S
S
Department of Pathology, Shiga University of Medical Science, Ohtsu, Japan.
Namura
K
K
Fujita
S
S
Kushima
R
R
Hattori
T
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000235:genetics; D002675:Child, Preschool; D004247:DNA / Q000032:analysis*; D004848:Epithelium / Q000473:pathology; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007399:Interphase; D008168:Lung / Q000032:analysis / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011003:Ploidies; D011123:Polyploidy
D004247:DNA