title: Effect of alimentary thiamine deficiency on the activity of gluconeogenic key enzymes in rat liver and kidney.
abstract: Activity of the key enzymes of gluconeogenesis under alimentary thiamine deficiency (15 days of dietary treatment) was studied in the liver and kidney of fed and 48 h starved rats. As compared to pair-fed controls vitamin B1-deficiency was followed by a decrease of glucose 6-phosphatase and fructose 1,6-bisphosphatase activities in both organs; the activity of phosphoenolpyruvate carboxykinase was diminished only in the liver. Starvation of thiamine-deficient rats (as compared to pair-fed starved group) resulted in lower activation of these enzymes. The decrease of the enzyme activities in thiamine-deficient animals indicates that de novo glucose synthesis in the tissues is depressed, though thiamine-requiring enzymes are not directly involved in this process. Possible mechanisms of alterations described are discussed.
authors.affiliation: Chair of Biochemistry, Medical Institute, Grodno, B. S. S. R., U. S. S. R.
authors.forename: A A
authors.initials: AA
authors.lastname: Maslovskaya
authors.forename: N K
authors.initials: NK
authors.lastname: Lukashik
mesh_terms: D000818:Animals; D004435:Eating; D005215:Fasting; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D005943:Gluconeogenesis; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism; D051381:Rats; D012016:Reference Values; D013832:Thiamine Deficiency / Q000201:enzymology*
chemical_list: D006597:Fructose-Bisphosphatase; D005952:Glucose-6-Phosphatase; D010729:Phosphoenolpyruvate Carboxykinase (GTP)