A physical map of the human PI and AACT genes.
We have used probes from the human genes PI, PIL, and AACT (alpha 1-antitrypsin, alpha 1-antitrypsin-related sequence, and alpha 1-antichymotrypsin) to make a pulsed-field map of the surrounding region of 14q31-32. We have discovered that the PI-PIL gene cluster is only 220 kb away from the AACT gene and that it is orientated in the opposite direction. The comparatively short distance between the genes comes as a surprise given previous estimates of the level of genetic recombination between them.
Sefton
L
L
Department of Genetics and Biometry, University College London, United Kingdom.
Kelsey
G
G
Kearney
P
P
Povey
S
S
Wolfe
J
J
D001426:Bacterial Proteins; D015139:Blotting, Southern; D002460:Cell Line; D002883:Chromosomes, Human, Pair 14; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005796:Genes; D008040:Genetic Linkage; D006801:Humans; D005810:Multigene Family; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic; D015183:Restriction Mapping; D000514:alpha 1-Antichymotrypsin / Q000235:genetics*; D000515:alpha 1-Antitrypsin / Q000235:genetics*
D001426:Bacterial Proteins; D000514:alpha 1-Antichymotrypsin; D000515:alpha 1-Antitrypsin; C035041:endodeoxyribonuclease MluI; D015252:Deoxyribonucleases, Type II Site-Specific