[Antioxidant effect of berbamine].
The antiperoxidant action of berbamine (Ber) was demonstrated by colorimetric estimation of malondialdehyde (MDA) formation, and the scavenging effect of Ber on O2- was investigated by chemiluminescence method and ESR-spin trapping technique. Ber 11-100 mumol/L remarkably inhibited MDA formation induced by incubating mice liver homogenate at 37 degrees C with vibration for 1 h. Ber 1-100 mumol/L and 0.2-0.6 mmol/L effectively scavenged O2- in alkaline DMSO and xanthine/xanthine oxidase systems respectively. The results show that Ber is an antioxidant.
Ju
H S
HS
Department of Pharmacology, Inner Mongolia Medical College, Huhehot, China.
Han
Z W
ZW
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants; D044182:Benzylisoquinolines; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers; D005609:Free Radicals; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008163:Luminescent Measurements; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D010100:Oxygen / Q000378:metabolism
D000470:Alkaloids; D000975:Antioxidants; D044182:Benzylisoquinolines; D016166:Free Radical Scavengers; D005609:Free Radicals; D008315:Malondialdehyde; D010100:Oxygen; C027870:berbamine