title: Developmentally regulated protein-tyrosine kinase genes in Dictyostelium discoideum.
abstract: Dictyostelium discoideum, an organism that undergoes development and that is amenable to biochemical and molecular genetic approaches, is an attractive model organism with which to study the role of tyrosine phosphorylation in cell-cell communication. We report the presence of protein-tyrosine kinase genes in D. discoideum. Screening of a Dictyostelium cDNA expression library with an anti-phosphotyrosine antibody identifies fusion proteins that exhibit protein-tyrosine kinase activity. Two distinct cDNAs were identified and isolated. Though highly homologous to protein kinases in general, these kinases do not exhibit many of the hallmarks of protein-tyrosine kinases of higher eucaryotes. In addition, these genes are developmentally regulated, which suggests a role for tyrosine phosphorylation in controlling Dictyostelium development.
authors.affiliation: Department of Cell Biology, Stanford University School of Medicine, California 94305.
authors.forename: J L
authors.initials: JL
authors.lastname: Tan
authors.forename: J A
authors.initials: JA
authors.lastname: Spudich
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D004023:Dictyostelium / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D015966:Gene Expression Regulation, Fungal; D005796:Genes; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011061:Poly A / Q000235:genetics; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000596:Amino Acids; D004271:DNA, Fungal; D012331:RNA, Fungal; D012333:RNA, Messenger; D011061:Poly A; D011505:Protein-Tyrosine Kinases