Some electrospray mass spectrometric evidence for the existence of covalent O-acyl enzyme intermediates.
Electrospray mass spectrometry has been used to measure the masses of the species present in solutions of three serine proteases (alpha-chymotrypsin, subtilisin Carlsberg and subtilisin BPN') before, during and after completion of the hydrolytic reaction with cinnamoyl imidazole and indole acryloyl imidazole. The masses measured during the reaction demonstrated that covalent O-acyl enzyme intermediates had been formed.
Ashton
D S
DS
Wellcome Research Laboratories, Beckenham, Kent, UK.
Beddell
C R
CR
Cooper
D J
DJ
Green
B N
BN
Oliver
R W
RW
Welham
K J
KJ
D002918:Chymotrypsin / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D008970:Molecular Weight; D013381:Subtilisins / Q000737:chemistry*
D013381:Subtilisins; D002918:Chymotrypsin