title: A case of B-CLL expressing P-glycoprotein in peripheral leukocytes. Methodological considerations.
abstract: P-glycoprotein, the molecular marker of multidrug resistance, was investigated by means of the specific monoclonal antibody C219 in circulating lymphocytes obtained from a patient affected by B-CLL, who had undergone chemotherapy with chlorambucil and CVP. Dot-immunobinding and immunoblotting revealed the presence of P-glycoprotein in the cell lysate, but the immunoperoxidase method and indirect immunofluorescence carried out with a fluorescence microscope and a cytofluorimeter failed to detect the protein. Some methodologic problems are discussed.
authors.affiliation: Servizio di Ematologia, Università, Pisa, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Carulli
authors.forename: M
authors.initials: M
authors.lastname: Petrini
authors.forename: F
authors.initials: F
authors.lastname: Vaglini
authors.forename: A
authors.initials: A
authors.lastname: Marini
authors.forename: F
authors.initials: F
authors.lastname: Ambrogi
authors.forename: B
authors.initials: B
authors.lastname: Grassi
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000368:Aged; D005260:Female; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood*; D007962:Leukocytes / Q000032:analysis*; D008562:Membrane Glycoproteins / Q000097:blood*; D009363:Neoplasm Proteins / Q000097:blood*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins