The inflammatory actions of platelet activating factor are blocked by levorotatory terbutaline.
Platelet activating factor (PAF), a potent vasoactive lipid, may play an important role in the inflammatory process. In this study, we infused PAF intra-arterially to characterize its edematogenic potency in the canine forelimb. We have also assessed the ability of the beta 2-receptor agonist l-terbutaline to block PAF-mediated edema formation. The infusion of PAF at .25 micrograms/min, .5 micrograms/min and 1 micrograms/min increased forelimb arterial pressures and, at the two higher dosages, significantly decreased systemic arterial pressure. PAF infusions increased transvascular fluid and macromolecular flux as indicated by significant increases in skin lymph flow, protein concentration and protein transport. The intra-arterial infusion of l-terbutaline at 1 micrograms/min significantly decreased forelimb arterial pressures but did not affect small vein pressure, systemic pressure or forelimb lymph parameters. The subsequent infusion of PAF at .5 micrograms/min, during the continued infusion of l-terbutaline, failed to significantly affect forelimb lymph parameters. These data indicate that PAF is significantly more potent as an edematogenic agent in the forelimb than histamine or bradykinin. Furthermore, the blockade of PAF-mediated edema formation by l-terbutaline suggests that beta 2-receptor agonists may be capable of antagonizing the inflammatory actions of a wide variety of putative inflammatory mediators.
Dobbins
D E
DE
Department of Physiology Uniformed Services University of the Health Sciences Bethesda, Maryland 20814-4799.
Buehn
M J
MJ
Dabney
J M
JM
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001920:Bradykinin / Q000494:pharmacology; D004285:Dogs; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D006632:Histamine / Q000494:pharmacology; D007249:Inflammation / Q000139:chemically induced / Q000517:prevention & control*; D007269:Injections, Intra-Arterial; D008196:Lymph / Q000737:chemistry; D008208:Lymphatic System / Q000187:drug effects*; D008968:Molecular Conformation; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors* / Q000633:toxicity; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000502:physiology; D013726:Terbutaline / Q000494:pharmacology*
D010972:Platelet Activating Factor; D011943:Receptors, Adrenergic, beta; D006632:Histamine; D013726:Terbutaline; D001920:Bradykinin