The transactivating domain of the c-Jun proto-oncoprotein is required for cotransformation of rat embryo cells.
The nuclear phosphoprotein c-Jun, encoded by the proto-oncogene c-jun, is a major component of the AP-1 complex. A potent transcriptional regulator, c-jun is also able to transform normal rat embryo cells in cooperation with an activated c-Ha-ras gene. By deletion analysis, we identified the regions of c-Jun encoding transformation and transactivation functions. Our studies indicate that there is a direct correlation between the ability of the c-Jun protein to activate transcription and cotransform rat embryo cells. The regions involved in these functions include the conserved leucine zipper/DNA binding domain and an effector domain near its N terminus. This N-terminal region spans amino acids 61 to 146 of the c-Jun protein and is highly conserved among all Jun family members. These results support the hypothesis that c-Jun transforms cells by stimulating the expression of transformation-mediating genes.
Alani
R
R
NCI-Navy Medical Oncology Branch, Bethesda, Maryland.
Brown
P
P
Binétruy
B
B
Dosaka
H
H
Rosenberg
R K
RK
Angel
P
P
Karin
M
M
Birrer
M J
MJ
D000818:Animals; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004247:DNA; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D015533:Transcriptional Activation
D016755:Proto-Oncogene Proteins c-jun; D004247:DNA