Effect of age on upregulation of the cardiac adrenergic beta receptors.
Radioligand binding studies were performed to determine whether upregulation of postjunctional beta receptors occurs in sympathectomized hearts of aged animals. Fischer 344 rats 6, 12, and 24 months of age (n = 10) were used in these experiments. To produce sympathectomy, rats were injected with 6-hydroxydopamine hydrobromide (6-OHDA; 2 x 50 mg/kg iv) on days 1 and 8; the animals were decapitated on day 15. The depletion of norepinephrine in the heart was about 86% in each age group. 125I-Iodopindolol (IPIN), a beta adrenergic receptor antagonist, was employed to determine the affinity and total number of beta adrenergic receptors in the ventricles of the rat heart. The maximal number of binding sites (Bmax) was significantly elevated by 37%, 48%, and 50% in hearts from sympathectomized 6-, 12-, and 24-month-old rats, respectively. These results indicate that beta receptor mechanisms in older hearts can respond to procedures that cause upregulation of the beta adrenergic receptors.
Tumer
N
N
Department of Pharmacology, Medical College of Pennsylvania.
Houck
W T
WT
Roberts
J
J
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000375:Aging / Q000502:physiology*; D000818:Animals; D006321:Heart / Q000294:innervation*; D006892:Hydroxydopamines / Q000008:administration & dosage / Q000494:pharmacology; D007457:Iodine Radioisotopes; D008297:Male; D009206:Myocardium / Q000032:analysis; D009638:Norepinephrine / Q000032:analysis; D016627:Oxidopamine; D010869:Pindolol / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D011943:Receptors, Adrenergic, beta / Q000032:analysis / Q000187:drug effects / Q000502:physiology*; D013562:Sympathectomy; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology*
D000319:Adrenergic beta-Antagonists; D006892:Hydroxydopamines; D007457:Iodine Radioisotopes; D011943:Receptors, Adrenergic, beta; C029247:3-iodopindolol; D016627:Oxidopamine; D010869:Pindolol; D009638:Norepinephrine