The gltF gene of Klebsiella pneumoniae: cloning and initial characterization.
From a gene bank of Klebsiella pneumoniae M5a1, a 1.7 kb gene fragment was isolated which was able to restore the Ntr+ phenotype and ammonium (methylammonium) transport, but not glutamate synthase in an Escherichia coli glt mutant (glutamate synthase deficiency). The fragment strongly hybridized with the gltF regulatory gene from E. coli. After subcloning the fragment into an overexpression vector, a protein with a molecular weight of 27,000 dalton was identified as the gene product. The results indicate that the fragment cloned contains the gltF gene from K.pneumoniae.
Kuczius
T
T
Lehrstuhl für Mikrobiologie, Universität Bayreuth, FRG.
Eitinger
T
T
D'Ari
R
R
Castorph
H
H
Kleiner
D
D
D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D005970:Glutamate Synthase / Q000235:genetics*; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics*; D016254:Mutagenesis, Insertional; D009154:Mutation; D015183:Restriction Mapping
D005970:Glutamate Synthase