[Identification of a protein product of a novel human gene SON and the biological effect upon administering a changed form of this gene into mammalian cells].
We have investigated the functional activity of human son gene, that possesses the homology to mos and myc genes. Specific antibodies (antiserum) were raised to synthetic peptide, that corresponds to son-protein 943-963 amino acid residues. With this antiserum the presence of son-protein was showed in lysates of cultured human cells transformed by adenovirus type 5, RAT 2 cells and primary human embryonic fibroblasts. son-Protein molecular weight (92 kDa) was determined by the method of electrophoresis in SDS-polyacrylamide gel. Thus, it was shown the presence of son gene protein in animal and human cells. To determine a possible son gene role in mammalian cells we have cloned the 3' part (2667 b.p.) of son cDNA in retroviral vector pPS-3-neo. Transformed cells of different lines were selected. A large portion of this cells changed their morphology. New protein product (120 k), that reacted with antiserum to son specific peptide, was found together with p92son in these clones.
Chumakov
I M
IM
Berdichevskiĭ
F B
FB
Sokolova
N V
NV
Reznikov
M V
MV
Prasolov
V S
VS
D016475:3T3 Cells; D000256:Adenoviridae; D000595:Amino Acid Sequence; D000818:Animals; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008322:Mammals; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D051381:Rats; D014162:Transfection
Identifikatsiia belka-produkta novogo gena cheloveka SON i biologichekiĭ éffekt pri vvedenii izmenennoĭ formy étogo gena v kletki mlekopitaiushchikh.