Preparation of specific monoclonal antibodies against Der f II one of the major allergens of Dermatophagoides farinae.
Five monoclonal antibodies (Mabs) against Der f II, one of the major allergens of Dermatophagoides farinae, were obtained from mice immunized with the allergen. Western blotting analysis revealed that only one Mab, named 15E11, was specific for Der f II, whereas the other Mabs crossreacted with Der p II, one of the major allergens of Dermatophagoides pteronyssinus. These results indicate the possibility that the Der f II-specific Mab, 15E11, is a useful reagent for specific determination of Der f II.
Akagawa
M
M
Biochemistry Section, Applied Technology Research Laboratories, Asahi Breweries, Ltd.
Mori
T
T
Ando
T
T
Okudaira
H
H
D000485:Allergens / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000918:Antibody Specificity; D015153:Blotting, Western; D008297:Male; D051379:Mice; D008925:Mites / Q000276:immunology*
D000485:Allergens; D000911:Antibodies, Monoclonal