title: Extracellular release of protease III (ptr) by Escherichia coli K12.
abstract: Escherichia coli strains carrying the protease III structural gene (ptr) on a plasmid secreted the protein into the growth medium. Plasmid-encoded beta-lactamase and chloramphenicol acetyl transferase, which served as periplasmic and cytoplasmic markers during cell fractionation, were not released into the growth medium. There appeared to be some strain dependence on the proficiency of the secretion system. Protease III was not detectably processed upon export through the outer cell membrane.
authors.affiliation: Department of Genetics, University of Georgia, Athens 30602.
authors.forename: M R
authors.initials: MR
authors.lastname: Díaz-Torres
authors.forename: C C
authors.initials: CC
authors.lastname: Dykstra
authors.forename: F
authors.initials: F
authors.lastname: Claverie-Martín
authors.forename: S R
authors.initials: SR
authors.lastname: Kushner
mesh_terms: D015153:Blotting, Western; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D005798:Genes, Bacterial; D008666:Metalloendopeptidases; D010957:Plasmids / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism
chemical_list: D021382:Protein Sorting Signals; D015500:Chloramphenicol O-Acetyltransferase; D010450:Endopeptidases; D008666:Metalloendopeptidases; C046583:pitrilysin; D001618:beta-Lactamases