title: Oxytetracycline production in solid state and submerged fermentation by protoplast fusants of Streptomyces rimosus.
abstract: Streptomyces rimosus TM-55 was treated with 3% EMS, and 29 auxotrophic mutants (AM-1 - AM-29) were isolated from 5457 colonies at a survival rate between 6.6-66.7%. Three sets of the auxotrophic mutants, AM-3 and AM-27, AM-7 and AM-28, and AM-3 and AM-21, were chosen for protoplast fusion with 50% PEG 1000 for 30 minutes at 25 degrees C, and 25 fusants were isolated (f-1 - f-25). In solid substrate, oxytetracycline production of 20% fusants was higher than that of the wild strain, while in submerged fermentation, it was 44%. Oxytetracycline productions of fusants f-1, f-6, f-11, f-12, f-20 and f-21 were lower than that of the wild strain in solid substrate, but this was reversed in submerged fermentation. On the other hand, OTC production of fusant f-8 was higher than that of the wild strain in solid substrate, and this was reversed in submerged fermentation.
authors.affiliation: Department of Agricultural Chemistry, National Taiwan University, Taipei, Republic of China.
authors.forename: S S
authors.initials: SS
authors.lastname: Yang
authors.forename: C Y
authors.initials: CY
authors.lastname: Kao
mesh_terms: D005285:Fermentation; D008561:Membrane Fusion / Q000187:drug effects; D009154:Mutation; D010118:Oxytetracycline / Q000096:biosynthesis*; D011092:Polyethylene Glycols / Q000494:pharmacology; D011523:Protoplasts / Q000378:metabolism*; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D011092:Polyethylene Glycols; D010118:Oxytetracycline