Isolation of fetal trophoblast cells from peripheral blood of pregnant women.
Fetal trophoblast cells were isolated from maternal peripheral blood by means of murine monoclonal antibodies of high specificity and affinity for human syncytiotrophoblast and nonvillous cytotrophoblast cells. The cells were isolated in sufficient numbers to allow polymerase chain reaction (PCR) amplification of the Y-chromosome-specific DNA sequence from the peripheral blood of thirteen pregnant women. The fetal sex predicted by PCR analysis of the isolated trophoblast cells accorded with that ascertained by karyotyping of chorionic villus samples in eleven of twelve women studied in early pregnancy and with the sex of the baby on delivery in one woman studied at 34 weeks' gestation. Isolation of these fetal cells could allow noninvasive diagnosis of a wide range of inherited disorders.
Mueller
U W
UW
Department of Obstetrics and Gynaecology, Flinders University of South Australia, Bedford Park.
Hawes
C S
CS
Wright
A E
AE
Petropoulos
A
A
DeBoni
E
E
Firgaira
F A
FA
Morley
A A
AA
Turner
D R
DR
Jones
W R
WR
D000911:Antibodies, Monoclonal; D002469:Cell Separation; D015193:Chorionic Villi Sampling; D004247:DNA / Q000032:analysis*; D005069:Evaluation Studies as Topic; D005189:False Positive Reactions; D005260:Female; D030342:Genetic Diseases, Inborn / Q000175:diagnosis; D006801:Humans; D007231:Infant, Newborn; D007621:Karyotyping; D008297:Male; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011247:Pregnancy / Q000097:blood* / Q000235:genetics; D011261:Pregnancy Trimester, First; D011263:Pregnancy Trimester, Third; D012732:Sex Determination Analysis; D014327:Trophoblasts / Q000166:cytology*; D014998:Y Chromosome / Q000032:analysis*
D000911:Antibodies, Monoclonal; D015345:Oligonucleotide Probes; D004247:DNA