title: Impact of L-phenylalanine supplementation on the performance of three-week-old broilers fed diets containing ochratoxin A. 2. Effects on hematology and clinical chemistry.
abstract: Phenylalanine was evaluated for its ability to protect broiler chickens from the toxic effects of ochratoxin A (OA). A completely randomized 2-by-3 factorial design was utilized consisting of 0, .8, and 2.4% supplemental L-phenylalanine (Phe) and of 0 and 4 mg of OA per kg of diet. The basal diet contained 14% protein. Broilers were raised in battery brooders to 3 wk of age, when blood was collected and various hematological parameters were determined. The health status of the broilers was evaluated by assaying serum for various enzyme activities and metabolites using an automated, clinical chemistry analyzer. Adding OA to the broiler diets resulted in an increased concentration of serum hemoglobin as well as increased activity for cholinesterase and gamma glutamyl transferase but in decreased activity for aspartate amino transferase, lactate dehydrogenase, and alkaline-phosphatase activity as well as decreased concentrations of total triglyceride and of inorganic phosphorus. Supplemental Phe decreased the concentrations of hemoglobin and serum glucose. The regression slopes for Phe at 4 mg of OA per kg of diet were significant for uric acid, creatinine, total protein, albumin, and cholesterol suggesting that supplemental Phe improved the health status of the broilers fed diets containing OA with respect to these parameters.
authors.affiliation: Department of Poultry Science, Texas A&M University, College Station 77843.
authors.forename: C A
authors.initials: CA
authors.lastname: Bailey
authors.forename: R M
authors.initials: RM
authors.lastname: Gibson
authors.forename: L F
authors.initials: LF
authors.lastname: Kubena
authors.forename: W E
authors.initials: WE
authors.lastname: Huff
authors.forename: R B
authors.initials: RB
authors.lastname: Harvey
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001786:Blood Glucose / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis; D002645:Chickens / Q000097:blood*; D002784:Cholesterol / Q000097:blood; D002802:Cholinesterases / Q000097:blood; D003404:Creatinine / Q000097:blood; D004347:Drug Interactions; D006454:Hemoglobins / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D009793:Ochratoxins / Q000506:poisoning*; D010649:Phenylalanine / Q000494:pharmacology*; D011897:Random Allocation; D012044:Regression Analysis; D012709:Serum Albumin / Q000032:analysis; D014527:Uric Acid / Q000097:blood; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D001786:Blood Glucose; D001798:Blood Proteins; D006454:Hemoglobins; D009793:Ochratoxins; D012709:Serum Albumin; C025589:ochratoxin A; D014527:Uric Acid; D010649:Phenylalanine; D002784:Cholesterol; D003404:Creatinine; D007770:L-Lactate Dehydrogenase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D002802:Cholinesterases; D000469:Alkaline Phosphatase