Preservation of rabbit kidneys using a solution containing hydrolyzed starch.
An organ preservation solution has been developed by combining some features of the hypertonic citrate formulation of Ross, Marshall, and Escott (RME) with some features of UW solution. Specifically the solution (HP16) contains a balance of cations similar to that in RME and the same concentration of citrate, but sulfate is replaced by chloride and mannitol by a starch hydrolysis product (SHP). A gelatin-derived polypeptide (Haemaccel) is included to provide colloid osmotic pressure. The objective was to increase the effectiveness of RME by using a higher-molecular-weight osmoticum than mannitol, but avoiding the expense of raffinose; reducing the osmolality to a more physiological level; and including a colloid to make the solution suitable for continuous perfusion. The effectiveness of the solution was tested by 48-hr hypothermic preservation of rabbit kidneys. The results were compared with those obtained using RME or UW. It was shown that simple hypothermic storage was more effective than continuous perfusion, and that HP16 was more effective than RME and as effective as UW. The improvement over RME was ascribed to the isotonic osmolality and the inclusion of a higher-molecular-weight osmoticum (the SHP), possibly supplemented by the colloid (Haemaccel). Two SHP preparations, both with dextrose-equivalent values of approximately 35, were equally effective. These materials contain a standardized mixture of dextrose, maltose, and tri- and oligosaccharides, and have the osmotic properties of a trisaccharide. The results provide a new, inexpensive preservation solution that is as effective as any so far tested with this model, and they support the importance of appropriate osmotic properties for solutions to be used in organ preservation.
Nørby
J
J
Laboratory of Nephropathology, Odense University, Denmark.
Jacobsen
I A
IA
Pegg
D E
DE
Starklint
H
H
Chemnitz
J
J
Diaper
M P
MP
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D003404:Creatinine / Q000097:blood; D005978:Glutathione; D007328:Insulin; D007678:Kidney Glomerulus / Q000187:drug effects / Q000648:ultrastructure; D016030:Kidney Transplantation; D008855:Microscopy, Electron, Scanning; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D011310:Preservatives, Pharmaceutical; D011817:Rabbits; D011887:Raffinose; D012996:Solutions; D013213:Starch
D007328:Insulin; D019188:Organ Preservation Solutions; D011310:Preservatives, Pharmaceutical; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D013213:Starch; D003404:Creatinine; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose