Analysis and characterization of the interleukin 2 receptor alpha and beta chain expression in CD4-CD8- cells.
The differential effects that the binding of interleukin 2 (IL-2) to its beta or alpha beta receptors might induce in two different CD4-CD8- T-cell lines were analysed. While LD1.T3b, a double-negative T cell derived from MRL/lpr mice, constitutively expressed high levels of the IL-2R beta chain, YAC-1, a Moloney sarcoma virus-transformed CD4-CD8- T cell, expressed (as an activated T cell) the beta and alpha chains. The presence of IL-2 in the culture medium was lethal for LD1.T3b cells, while it had no effect on the growth of YAC-1 cells. IL-2 increased the expression of the beta chain and, to a lesser extent, of the alpha chain in YAC-1 cells. In addition, other markers such as CD4 and CD5 were induced by IL-2 in this cell line.
Gutiérrez-Ramos
J C
JC
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma de Madrid, Spain.
Pezzi
L
L
Leonardo
E
E
Martin
C
C
Martínez
C
C
D015496:CD4-Positive T-Lymphocytes / Q000032:analysis* / Q000276:immunology; D002460:Cell Line; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D010641:Phenotype; D015375:Receptors, Interleukin-2 / Q000032:analysis* / Q000235:genetics; D050378:T-Lymphocytes, Regulatory / Q000032:analysis* / Q000276:immunology
D007376:Interleukin-2; D015375:Receptors, Interleukin-2