title: A human colon cancer cell line established from collagen matrix cultures transplanted into nude mice.
abstract: A new human colon cancer cell line (020588) has been derived by means of a combined in vitro matrix-in vivo xenograft technique. The tumour cell line is carcino-embryonic antigen positive, displays a marker chromosome and proliferates in chemically-defined serum-free culture medium. The chemosensitivity pattern for the tumour cell line was similar to that observed for the parent tumour cells. The novel method used to establish this continuous human tumour cell line may have several advantages over standard techniques.
authors.affiliation: Faculty of Medicine, University of Newcastle, New South Wales, Australia.
authors.forename: M V
authors.initials: MV
authors.lastname: Agrez
authors.forename: F K
authors.initials: FK
authors.lastname: Chua
authors.forename: K
authors.initials: K
authors.lastname: Fagan
authors.forename: J W
authors.initials: JW
authors.lastname: Heath
authors.forename: N W
authors.initials: NW
authors.lastname: Ferguson
mesh_terms: D000818:Animals; D002272:Carcinoembryonic Antigen / Q000302:isolation & purification; D002460:Cell Line; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D003094:Collagen; D003110:Colonic Neoplasms; D016895:Culture Media, Serum-Free; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D016685:Mitomycin / Q000494:pharmacology; D009368:Neoplasm Transplantation
chemical_list: D002272:Carcinoembryonic Antigen; D016895:Culture Media, Serum-Free; D016685:Mitomycin; D003094:Collagen; D005472:Fluorouracil