Characterization of the pilin gene of Moraxella bovis Dalton 2d and expression of pili from M. bovis in Pseudomonas aeruginosa.
The pilin gene of Moraxella bovis Dalton 2d was isolated by cloning in Pseudomonas aeruginosa. The nucleotide sequence of this gene encodes a prepilin of 156 amino acid residues. When high levels of pilin were expressed from the gene in P. aeruginosa, by using the pL promoter of bacteriophage lambda inserted upstream of the coding sequence, pili which were indistinguishable from pili of M. bovis were produced.
Elleman
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Division of Biotechnology, Commonwealth Scientific and Industrial Research Organisation, Parkville, Victoria, Australia.
Hoyne
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Lepper
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D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D015153:Blotting, Western; D002458:Cell Fractionation; D003001:Cloning, Molecular; D015342:DNA Probes; D004926:Escherichia coli / Q000235:genetics; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000276:immunology; D015870:Gene Expression; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009016:Moraxella / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000235:genetics
D001425:Bacterial Outer Membrane Proteins; D015342:DNA Probes; D036781:Fimbriae Proteins