Heterogeneity in Alzheimer's disease: evidence from cellular radiosensitivity and complementation of this phenotype.
Radiosensitivity was studied in a series of Alzheimer's disease (AD) patients and normal controls by examining clonogenic survival and radiation-induced chromosome aberrations in lymphoblastoid cell lines. D0 values based on colony survival for AD and normals following exposure to gamma-rays were 0.86 +/- 0.04 and 1.14 +/- 0.03 Gy respectively. However, 2 of the AD cell lines had D0 values in the normal range. This increased radiosensitivity in AD cells was confirmed by an increased number of gamma-ray-induced chromosome aberrations in these cells. Cell fusion was employed to investigate the presence of different complementation groups for the radiosensitive phenotype in AD using frequency of radiation-induced chromosome aberrations as a means of distinguishing different groups. Four complementation groups were found among 5 AD cell lines. These findings provide additional experimental evidence in support of heterogeneity in AD.
Chen
P
P
Queensland Institute of Medical Research, Brisbane, Australia.
Kidson
C
C
Lavin
M
M
D000368:Aged; D000544:Alzheimer Disease / Q000097:blood / Q000235:genetics*; D002459:Cell Fusion / Q000235:genetics; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000528:radiation effects; D002869:Chromosome Aberrations; D002999:Clone Cells / Q000528:radiation effects; D004247:DNA / Q000528:radiation effects; D005260:Female; D005720:Gamma Rays; D005816:Genetic Complementation Test; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011836:Radiation Tolerance / Q000235:genetics*
D004247:DNA