PCR detection of distal Yp sequences in an XX true hermaphrodite.
An XX true hermaphrodite was examined for the presence of Y-specific sequences using Southern-blotting and polymerase chain reaction (PCR) techniques. Of 25 loci examined, only two, the proximal border of the pseudoautosomal region (PABY) and the sex determining region of the Y chromosome (SRY), were detected. A crossing over event in paternal meiosis, proximal to the SRY locus but distal to the zinc finger protein (ZFY) locus, presumably transferred to two loci to the X chromosome.
Nakagome
Y
Y
Department of Congenital Abnormalities Research, National Children's Medical Research Center, Tokyo, Japan.
Seki
S
S
Fukutani
K
K
Nagafuchi
S
S
Nakahori
Y
Y
Tamura
T
T
D000293:Adolescent; D000595:Amino Acid Sequence; D015139:Blotting, Southern; D003434:Crossing Over, Genetic / Q000235:genetics*; D012734:Disorders of Sex Development / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D016133:Polymerase Chain Reaction; D012732:Sex Determination Analysis; D014960:X Chromosome; D014998:Y Chromosome
D009838:Oligodeoxyribonucleotides