Effects of transforming growth factor beta 1 on astroglial cells in culture.
The effects of transforming growth factor beta 1 (TGF beta 1) on DNA synthesis and functional differentiation of astroglial cells cultured in serum-free medium were investigated. TGF beta 1 diminished and delayed the peak of DNA synthesis induced by serum. TGF beta 1-treated cells were larger than control cells. This factor delayed the appearance of process-bearing cells induced by acidic fibroblast growth factor treatment and also affected the astrocyte-specific enzyme glutamine synthetase (GS), whose accumulation is under hydrocortisone (HC) control. TGF beta 1 inhibited the induction of GS activity by HC in a dose- and time-dependent manner. Moreover, pretreatment with TGF beta 1 for 4 h maintained the inhibition of GS activity for approximately 16 h after removal of this factor from culture medium. These results suggest that TGF beta 1 may be an important regulator of astrocyte growth and differentiation.
Toru-Delbauffe
D
D
INSERM U. 96, Hôpital Kremlin-Bicêtre, France.
Baghdassarian-Chalaye
D
D
Gavaret
J M
JM
Courtin
F
F
Pomerance
M
M
Pierre
M
M
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology; D013997:Time Factors; D015291:Transforming Growth Factors / Q000494:pharmacology*
D015291:Transforming Growth Factors; D004247:DNA; D005974:Glutamate-Ammonia Ligase; D006854:Hydrocortisone