Phosphate-dependent sodium transport in S. faecalis investigated by 23Na and 31P NMR.
Na+ movements in S. faecalis were studied by 23Na NMR. They proved to be dependent on phosphate concentration in the buffer during the de-energization step. K+ and H+ were also studied respectively by potentiometry and 31P NMR and were shown not to be implicated. For de-energized cells the internal phosphate concentration, on the contrary, was directly linked to the external phosphate contained in the buffer. The experiments showed a Na+/Pi dependence in this prokaryote so far known only in eukaryotes.
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Université Blaise Pascal Clermont-Ferrand, Laboratoire de Chimie Organique Biologique-U.R.A., 485 du C.N.R.S., Aubière, France.
Dauphin
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Jeminet
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Guyot
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J
Delort
A M
AM
D001692:Biological Transport / Q000187:drug effects; D002794:Choline / Q000494:pharmacology; D013293:Enterococcus faecalis / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D010710:Phosphates / Q000494:pharmacology*; D010758:Phosphorus; D011188:Potassium / Q000378:metabolism; D011199:Potentiometry; D012964:Sodium / Q000378:metabolism*; D013054:Spectrophotometry, Atomic
D010710:Phosphates; D010758:Phosphorus; D012964:Sodium; D002794:Choline; D011188:Potassium