title: Application of immunocytochemistry to the cytologic study of peritoneal fluids in patients with ovarian cancer.
abstract: Immunocytochemical methods were evaluated for their usefulness in the identification of malignant cells in cytologic preparations of peritoneal fluids of ovarian cancer. The antibodies used for this study were anti-cancer antigen-125 (CA-125) antibody, anti-carbohydrate antigen 19-9 (CA 19-9) antibody, anti-carcinoembryonic antigen (CEA) antibody and anti-epithelial membrane antigen (EMA) antibody. The immunohistochemical and cytochemical results using 4 antibodies for both the histologic specimens and the imprinted smears from tumor tissues of the same patients were compatible in 91.8% (both positive or both negative) of the cases. Reactivities of ascitic specimens taken from benign gynecological lesions were 39.4% with anti-CA-125, 12.1% with anti-CA 19-9, 6.1% with anti-EMA, while none of the 33 cases reacted with anti-CEA antibodies. Comparison of the immunoreactivity with the 4 antibodies in histologic specimens and peritoneal smears with Pap-positive cytology taken from the same patients showed 82.9% compatibility. However, 40% incompatibility was found in the anti-CA-125 staining. Some of the mesothelial cells contained in the peritoneal fluids reacted with the anti-CA-125 antibody. Except for the anti-CA-125 staining, compatibility was 90.3%. These results indicate that the anti-CA-125 antibody can not be used to distinguish benign from malignant cells, while anti-CEA antibody has high specificity for malignant cells. CA19-9, CEA and EMA stainings may be valuable criteria in the cytologic diagnosis of effusions. However, it is possible that individual use of these antibodies may lead to a false negative diagnosis.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Obstetrics and Gynecology, Tokai University School of Medicine, Kanagawa.
authors.forename: T
authors.initials: T
authors.lastname: Shinozuka
authors.forename: K
authors.initials: K
authors.lastname: Ebisawa
authors.forename: T
authors.initials: T
authors.lastname: Miyamoto
authors.forename: M
authors.initials: M
authors.lastname: Murakami
authors.forename: A
authors.initials: A
authors.lastname: Fujii
authors.forename: Y
authors.initials: Y
authors.lastname: Osamura
mesh_terms: D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis; D001202:Ascitic Fluid / Q000166:cytology* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000032:analysis*; D002272:Carcinoembryonic Antigen / Q000032:analysis; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000592:standards; D008562:Membrane Glycoproteins / Q000032:analysis; D018396:Mucin-1; D010051:Ovarian Neoplasms / Q000175:diagnosis*
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D008562:Membrane Glycoproteins; D018396:Mucin-1