title: Characterization of haemagglutinin from Bordetella bronchiseptica.
abstract: In comparison with haemagglutinin (HA)-active strains of Bordetella bronchiseptica, the HA-deficient strains lacked a 150 kDa protein band on SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Adsorption of partially purified HA with bovine erythrocytes showed the loss of the 150 kDa band in the supernatant. This 150 kDa protein was purified by high-performance liquid chromatography using gel filtration columns. Electron microscopic examination revealed that the purified HA possessed a fine filamentous structure with dimensions of approximately 2 x 150 nm, which was considered to represent the filamentous haemagglutinin of B. bronchiseptica. Both the cell-free antigen obtained from the culture supernatant of phase-I strain of the bacteria and the bovine erythrocytes, which combined with crude HA showed an excellent protective activity of the challenge by virulent strain of B. bronchiseptica in mice.
authors.affiliation: Division of Veterinary Microbiology, Kyoto Biken Laboratories, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohgitani
authors.forename: T
authors.initials: T
authors.lastname: Okabe
authors.forename: N
authors.initials: N
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification; D016950:Bordetella bronchiseptica / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006388:Hemagglutinins / Q000276:immunology / Q000302:isolation & purification*; D008297:Male; D051379:Mice
chemical_list: D000907:Antibodies, Bacterial; D006388:Hemagglutinins