In vitro expression of bovine leukemia virus in isolated B-lymphocytes of cattle and sheep.
The purpose of this study was to determine the effect of T-lymphocytes and phytohemagglutinin (PHA), a T-cell mitogen, on the expression of bovine leukemia virus (BLV) in cultured B-lymphocytes from BLV-infected cattle and sheep. Bovine B-lymphocytes were isolated by negative selection via complement-mediated lysis of T-lymphocytes. Additionally, bovine and ovine B-lymphocytes were positively selected using fluorescence activated cell sorting. Expression of BLV in cultured bovine and ovine B-lymphocytes occurred in the absence of T-lymphocytes and without PHA stimulation. The results of this study demonstrate that BLV replication in cultured B-lymphocytes is T-cell independent. This finding may have implications for the mechanism of viral latency within infected B-lymphocytes.
Jensen
W A
WA
Department of Pathology, Colorado State University, Fort Collins 80523.
Sheehy
S E
SE
Fox
M H
MH
Davis
W C
WC
Cockerell
G L
GL
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000382:microbiology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007938:Leukemia / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D001909:Leukemia Virus, Bovine / Q000187:drug effects / Q000254:growth & development*; D010835:Phytohemagglutinins / Q000494:pharmacology; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000382:microbiology; D014779:Virus Replication / Q000187:drug effects / Q000276:immunology
D000911:Antibodies, Monoclonal; D010835:Phytohemagglutinins