Effects of aluminum on tau proteins in human neuroblastoma cells.
The presence of the trivalent metallic cations, aluminum and boron, in the culture medium of differentiated human LAN-5 neuroblastoma cells results in increased amounts of specific isomers of microtubule-associated tau proteins. The cells were differentiated to a neuronal phenotype by the addition of retinoic acid. Six-day exposures of the differentiated cells to a 1-mM dose of aluminum or boron yielded increases in tau protein immunoreactivity to the monoclonal antibodies Tau-1 and Alz-50. Significant increases in immunoreactivity were seen at treatment levels of aluminum down to 100 microM. The increases in tau proteins were independent from increases in levels of total cell protein. Control cultures treated with the divalent cations zinc and iron showed no increases in levels of tau proteins.
Mesco
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Department of Molecular and Cellular Biology, University of California, Berkeley 94720.
Kachen
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Timiras
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D000535:Aluminum / Q000494:pharmacology*; D001895:Boron / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D007501:Iron / Q000494:pharmacology; D009447:Neuroblastoma / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D014407:Tumor Cells, Cultured; D015032:Zinc / Q000494:pharmacology; D016875:tau Proteins / Q000187:drug effects* / Q000378:metabolism
D016875:tau Proteins; D000535:Aluminum; D007501:Iron; D015032:Zinc; D001895:Boron