title: The distribution of homologues of the Escherichia coli KefC K(+)-efflux system in other bacterial species.
abstract: Using a variety of techniques the distribution of the glutathione-regulated KefC K(+)-transport system among bacterial species was investigated. The presence of similar systems in a number of Gram-negative bacteria was demonstrated. In contrast, the system appeared to be absent from most Gram-positive bacteria tested with the exception of Staphylococcus aureus. Using the cloned Escherichia coli kefC gene as a probe for Southern hybridization it was shown that only limited DNA sequence homology exists with other bacteria, even when closely related members of the enteric group were examined.
authors.affiliation: Department of Molecular and Cell Biology, University of Aberdeen, Marischal College, UK.
authors.forename: R M
authors.initials: RM
authors.lastname: Douglas
authors.forename: J A
authors.initials: JA
authors.lastname: Roberts
authors.forename: A W
authors.initials: AW
authors.lastname: Munro
authors.forename: G Y
authors.initials: GY
authors.lastname: Ritchie
authors.forename: A J
authors.initials: AJ
authors.lastname: Lamb
authors.forename: I R
authors.initials: IR
authors.lastname: Booth
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001693:Biological Transport, Active / Q000187:drug effects; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005033:Ethylmaleimide / Q000494:pharmacology; D005978:Glutathione / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000378:metabolism; D007700:Kinetics; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels; D012689:Sequence Homology, Nucleic Acid; D013211:Staphylococcus aureus / Q000378:metabolism; D013388:Succinimides / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D015342:DNA Probes; D029968:Escherichia coli Proteins; D015221:Potassium Channels; D013388:Succinimides; C069287:KefC protein, E coli; C064530:N-ethylsuccinimido-S-glutathione; D005978:Glutathione; D005033:Ethylmaleimide; D011188:Potassium