Alterations in glycolytic and oxidative potentials of rat brain during acute and chronic acephate treatments.
Alterations in the rat brain carbohydrate and related metabolisms were studied during acute and chronic acephate toxicity. The rats were divided into three batches of eight in each batch. The first batch was treated with chronic (50 mg.Kg-1.day-1 for 7 weeks) and second batch was treated with acute (600 mg.Kg-1.day-1 for one day) doses of acephate, third group was served as control which received vehicle only. The representative enzymes like SDH, MDH, LDH, GDH, AAT and AlAT activities were decreased significantly during chronic treatment. Whereas MDH, LDH, AAT and AlAT activities showed significant increase during acute treatment. The glycogen and pyruvate levels showed nonsignificant elevation and lactate and total carbohydrate levels were depleted in the brains of chronic acephate treated rats. Reverse trend was observed with regard to lactate and pyruvate during acute toxicity whereas the total carbohydrates and glycogen levels were significantly elevated. The decreased oxidative potential and reduced flux of ketoacids into TCA cycle through transamination reactions indicate that acephate caused energy crisis in the brain during chronic treatment. During acute treatment the inhibited succinate oxidation was compensated by the ketoacid contributions through transamination reactions. The neuro transmitter balance with particular reference to glutamate during toxic stress was reflected through the GDH levels in both the treatments.
Rao
P V
PV
Department of Zoology, Sri Venkateswara University, Tirupati, India.
Prasad
S J
SJ
Rajendra
W
W
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D009946:Organothiophosphorus Compounds / Q000633:toxicity*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D063088:Phosphoramides; D051381:Rats; D011919:Rats, Inbred Strains
D007306:Insecticides; D009946:Organothiophosphorus Compounds; D063088:Phosphoramides; C001969:acephate; D010088:Oxidoreductases