title: Tyrosine hydroxylase gene expression in human ventral mesencephalon: detection of tyrosine hydroxylase messenger RNA in neurites.
abstract: The distribution of both tyrosine hydroxylase (TH) antigen and messenger RNA was investigated in the ventral mesencephalon from human brain using a 35S-labeled human TH cDNA probe and a polyclonal TH antibody. A hybridization signal was detected not only in the perikaryon but also in the neurites, most probably dendrites, of immunoreactive neurons. In cell bodies, the level of TH mRNA varies, revealing a regional heterogeneity. In the substantia nigra of patients with Parkinson's disease the number of labeled cells was markedly reduced as compared to controls.
authors.affiliation: Laboratoire de Neurobiologie Cellulaire, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
authors.forename: S
authors.initials: S
authors.lastname: Dumas
authors.forename: F
authors.initials: F
authors.lastname: Javoy-Agid
authors.forename: E
authors.initials: E
authors.lastname: Hirsch
authors.forename: Y
authors.initials: Y
authors.lastname: Agid
authors.forename: J
authors.initials: J
authors.lastname: Mallet
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D004247:DNA; D003712:Dendrites / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D004247:DNA; D014446:Tyrosine 3-Monooxygenase