Epidemiological typing of Yersinia enterocolitica by analysis of restriction fragment length polymorphisms with a cloned ribosomal RNA gene.
Intra-species restriction fragment length polymorphisms (RFLPs) of Yersinia enterocolitica were detected in assays with a cloned DNA fragment from Legionella pneumophila that included the 16S and 23S rRNA genes. By use of this method it was possible to identify different RFLP types within biogroups/serogroups which were indistinguishable by other means. Thus the 37 biogroup IV/serogroup O3 strains isolated worldwide from pig carcasses, pork and human patients, were subdivided into five different RFLP-types. Typing based on RFLP analysis was sensitive and independent of phenotypic characters. The method will be of value for the identification and evaluation of possible reservoirs and routes of infection.
Andersen
J K
JK
Institute of Hygiene and Microbiology, Royal Veterinary and Agricultural University, Fredericksberg, Denmark.
Saunders
N A
NA
D000818:Animals; D001208:Asia / Q000453:epidemiology; D004269:DNA, Bacterial / Q000032:analysis; D015247:Deoxyribonuclease HindIII; D005060:Europe / Q000453:epidemiology; D006801:Humans; D009656:North America / Q000453:epidemiology; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D013552:Swine; D015009:Yersinia Infections / Q000453:epidemiology*; D015008:Yersinia enterocolitica / Q000145:classification* / Q000235:genetics
D004269:DNA, Bacterial; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D015247:Deoxyribonuclease HindIII