title: Purification of inorganic pyrophosphatase from rat salivary glands.
abstract: Inorganic pyrophosphatase [EC 3.6.1.1] isolated from rat sublingual and submandibular glands was purified 2300-fold and 2600-fold, respectively. The purified enzymatic preparations separated on electrophoresis into two protein bands, of which only one showed the pyrophosphatase activity. Inorganic pyrophosphatase from rat salivary glands is a monomeric anionic protein, its isoelectric point is 5.42 and 4.90 for the sublingual and submandibular glands, respectively.
authors.affiliation: Department of Biochemistry, Institute of Biopharmacy, Medical Academy in Warsaw, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Koskowska-Dziadowicz
authors.forename: M
authors.initials: M
authors.lastname: Ochman
authors.forename: T
authors.initials: T
authors.lastname: Szymczyk
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D043564:Inorganic Pyrophosphatase; D007525:Isoelectric Focusing; D008297:Male; D008970:Molecular Weight; D011755:Pyrophosphatases / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D013361:Sublingual Gland / Q000201:enzymology*; D013363:Submandibular Gland / Q000201:enzymology*
chemical_list: D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase