Intraperitoneally injected cultured mast cells suppress recruitment and differentiation of bone marrow-derived mast cell precursors in the peritoneal cavity of W/Wv mice.
Bone marrow-derived mast cell precursors form large mast cell colonies in methylcellulose and are designated as L-CFU-Mast. The effect of differentiated mast cells on recruitment and differentiation of L-CFU-Mast was investigated by using genetically mast cell-deficient WBB6F1-W/Wv mice. Giant granules of C57BL/6-bgJ/bgJ (Chediak-Higashi syndrome) mice were used as a marker to identify the origin of L-CFU-Mast and differentiated mast cells. Practically no L-CFU-Mast are present in the peritoneal cavity of WBB6F1-W/Wv mice. When bone marrow cells of WBB6F1(-)+/+ mice were i.v. injected, the concentration of +/+(-)type L-CFU-Mast increased in the peritoneal cavity of WBB6F1-W/Wv mice and became several times greater than that of nontreated WBB6F1(-)+/+ mice. This increase of L-CFU-Mast was suppressed by a prior i.p. injection of bgJ/bgJ-type cultured mast cells. The differentiation of the +/+(-)type L-CFU-Mast to morphologically identifiable mast cells was also suppressed by the i.p. injection of bgJ/bgJ-type cultured mast cells. The present results suggest that the suppression of recruitment and differentiation of L-CFU-Mast is a physiological function of differentiated mast cells.
Waki
N
N
Department of Pathology, Osaka University Medical School, Japan.
Kitamura
Y
Y
Kanakura
Y
Y
Asai
H
H
Nakano
T
T
D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002455:Cell Division; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D008407:Mast Cells / Q000166:cytology* / Q000502:physiology / Q000637:transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D010529:Peritoneal Cavity / Q000166:cytology*