The murine genes Hox-5.1 and Hox-4.1 belong to the same HOX complex on chromosome 2.
Two different loci of Antennapedia-related homeobox-containing genes have been shown to map to mouse chromosome 2: the HOX-5 complex and the Hox-4.1 gene. These independently derived loci are likely to be parts of a single gene complex, although their close linkage has not yet been demonstrated. Since cosmid walks to extend the HOX-5 cluster and to potentially link the two loci were unsuccessful, we have used large restriction fragments separated by pulsed-field gel electrophoresis to demonstrate the linkage between probes from the HOX-5 region and sequences near Hox-4.1. To further define the distance between the two linked loci, we screened a NotI jumping library with sequences near the Hox-5.1 gene to obtain a marker within the region predicted to contain Hox-4.1. The jumping endpoint lies within genomic clones from a lambda phage walk extending from the 5' end of Hox-4.1, and thus provides clear evidence of linkage between the two Hox loci. Our results demonstrate that Hox-4.1 lies approximately 35 kb downstream of the Hox-5.1 gene and that the two loci do indeed thus constitute parts of the same HOX complex.
Stubbs
L
L
Imperial Cancer Research Fund Laboratories, Lincoln's Inn Fields, London, United Kingdom.
Poustka
A
A
Baron
A
A
Lehrach
H
H
Lonai
P
P
Duboule
D
D
D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes; D003001:Cloning, Molecular; D003360:Cosmids; D004586:Electrophoresis; D005801:Genes, Homeobox; D008040:Genetic Linkage; D005819:Genetic Markers; D008297:Male; D051379:Mice; D005810:Multigene Family; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D014158:Transcription, Genetic
D005819:Genetic Markers