title: W (A or T) sequences as probes and primers suitable for genomic mapping and fingerprinting.
abstract: A limitation to the use of oligonucleotide probes as tools for genetic and physical mapping has been the low hybridization positive frequency obtained by oligonucleotides of sufficient length to hybridize preferentially to cloned insert DNA (and not host E. coli genomic DNA). Both computer and experimental results now indicate that oligonucleotide probes composed of W (A or T) sequence are preferentially found in eukaryotic DNA, and can be used to provide high frequency, discriminative hybridization. Such W sequences may be useful as either probes or PCR primers in molecular diagnostic applications as well as in genetic and physical mapping.
authors.affiliation: Genome Analysis Laboratory, Imperial Cancer Research Fund, London, UK.
authors.forename: R
authors.initials: R
authors.lastname: Drmanac
authors.forename: D
authors.initials: D
authors.lastname: Nizetic
authors.forename: G G
authors.initials: GG
authors.lastname: Lennon
authors.forename: A
authors.initials: A
authors.lastname: Beitverda
authors.forename: H
authors.initials: H
authors.lastname: Lehrach
mesh_terms: D000225:Adenine; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D003360:Cosmids / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000235:genetics; D015345:Oligonucleotide Probes / Q000235:genetics*; D013941:Thymine; D014960:X Chromosome
chemical_list: D015345:Oligonucleotide Probes; D000225:Adenine; D013941:Thymine