title: [Aggregation of tryptophanyl-tRNA synthetase depending on temperature. Study by a low-angle scatter x-ray method].
abstract: By means of small angle X-ray scattering, an aggregation of beef pancreas Trp-tRNA synthetase (EC 6.1.1.2) was observed at physiological temperatures. A Trp-tRNA synthetase preparation which is homogeneous after PAGE in beta-ME-SDS was found to be heterogeneous in particle sizes even at low (4-8 degrees C) temperature. At heating up to 30-45 degrees C, the oligomer sizes increased as well as its proportion depending on the incubation time and temperature; very large aggregates were observed 10 times exceeding the sizes of initial particles. Cooling to 20 degrees C caused no disaggregation due to disulphide bond formation between associated subunits of Trp-tRNA synthetase. A hypothesis is proposed that the aggregation of bovine Trp-tRNA synthetase evaluated in vitro and not observed earlier with any aminoacyl-tRNA synthetases of unicellular organisms might serve as one of the mechanisms of its compartmentation in pancreas.
authors.forename: F V
authors.initials: FV
authors.lastname: Tuzikov
authors.forename: N A
authors.initials: NA
authors.lastname: Tuzikova
authors.forename: V I
authors.initials: VI
authors.lastname: Vavilin
authors.forename: V V
authors.initials: VV
authors.lastname: Zinov'ev
authors.forename: E G
authors.initials: EG
authors.lastname: Malygin
authors.forename: O O
authors.initials: OO
authors.lastname: Favorova
authors.forename: T A
authors.initials: TA
authors.lastname: Zargarova
authors.forename: M A
authors.initials: MA
authors.lastname: Sudomoina
authors.forename: L L
authors.initials: LL
authors.lastname: Kiselev
mesh_terms: D000818:Animals; D002417:Cattle; D004220:Disulfides / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D013696:Temperature; D014369:Tryptophan-tRNA Ligase / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D004220:Disulfides; D014369:Tryptophan-tRNA Ligase
vernacular_title: Zavisiashchaia ot temperatury agregatsiia triptofanil-tRNK-sintetazy. Issledovanie metodom malouglovogo rentgenovskogo rasseianiia.