A differentiation stage-specific factor interacts with mouse carbonic anhydrase form I gene and a conserved sequence in mammalian beta-globin genes.
We have identified in mouse erythroleukemic (MEL) cells a novel factor which binds to the 3' flanking region of beta-globin genes. Upon induction, this DNA binding factor disappears as beta-globin gene transcription increases. The factor protects 20-30 base pairs (bp) of a sequence which is tightly conserved in beta-globin genes including chicken, human, mouse and rabbit. A very similar sequence binds the factor in the mouse carbonic anhydrase form I gene, whose transcription diminishes upon induction of MEL cells. The factor, or a closely related form, is detected in nonerythroid cells and binding sites have been detected in other genes, including c-myb and immunoglobulin heavy chain-enhancer. We suggest that this factor could play a role in determining the timing of gene expression in several differentiating cell types.
Ma
X J
XJ
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Fraser
P
P
Curtis
P J
PJ
D000818:Animals; D001482:Base Composition / Q000502:physiology; D001483:Base Sequence; D002256:Carbonic Anhydrases / Q000235:genetics*; D002455:Cell Division / Q000502:physiology; D002645:Chickens; D004268:DNA-Binding Proteins / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology; D005914:Globins / Q000235:genetics*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000503:physiopathology; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D014162:Transfection / Q000235:genetics
D004268:DNA-Binding Proteins; D005914:Globins; D002256:Carbonic Anhydrases