Reversal of murine colchicine toxicity by colchicine-specific Fab fragments.
High-affinity Fab fragments (2 x 10(10) M-1) specific to colchicine were produced to evaluate their potency in reversing murine colchicine intoxication. Intraperitoneal injection of a 4.46 mg/kg colchicine dose was lethal for 100% of mice. 1.5 h after colchicine administration, a group of 10 mice was treated with colchicine-specific Fab fragments at a half-stoichiometrical dose compared to the colchicine dose by intravenous and intraperitoneal routes. 70% of the Fab-infused mice survived (P less than 0.01). This high efficiency of colchicine-specific Fab fragments in reversing acute murine colchicine toxicity suggests that Fab fragments would be an efficient antidote for the treatment of human colchicine poisoning.
Urtizberea
M
M
INSERM U-26, Fernand Widal Hospital, Paris, France.
Sabouraud
A
A
Cano
N
N
Grandgeorge
M
M
Rouzioux
J M
JM
Scherrmann
J M
JM
D000818:Animals; D003078:Colchicine / Q000633:toxicity*; D007035:Hypothermia / Q000139:chemically induced / Q000188:drug therapy; D007128:Immunoglobulin Fragments / Q000008:administration & dosage*; D007074:Immunoglobulin G / Q000008:administration & dosage; D008297:Male; D051379:Mice
D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D003078:Colchicine