[Use of the polymerase chain reaction for analysis of rotaviruses. Nucleotide sequence of a gene, coding for the basic neutralizing antigen VR7 of a human rotavirus with a new G-serotype].
A procedure based on polymerase chain reaction use for the detection of rotavirus has been developed. Full length cDNA copy of the VP7 gene coding for the major neutralization glycoprotein of the human rotavirus RK9 with an unusual "wide" electrophoretype is cloned and sequenced. Glycoprotein VP7 of RK9 has a unique amino acid composition in A and C antigenic regions. It shows that strain RK9 represents a new (12) rotavirus serotype.
Bessarab
I N
IN
Novikova
N A
NA
Borodin
A M
AM
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics*; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D004247:DNA / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics* / Q000276:immunology; D012689:Sequence Homology, Nucleic Acid
D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus; D004247:DNA
Ispol'zovanie polimeraznoĭ tsepnoĭ reaktsii dlia analiza rotavirusov. Nukleotidnaia posledovatel'nost' gena, kodiruiushchego osnovnoĭ neĭtralizuiushchiĭ antigen VP7 rotavirusa cheloveka novogo G-serotipa.