Primary structure of the two variants of Xenopus laevis mtSSB, a mitochondrial DNA binding protein.
The primary structure of the single-stranded DNA binding protein from Xenopus laevis oocyte mitochondria (mtSSB) has been determined by Edman degradation of the intact molecule and peptides derived from partial alpha-chymotrypsin proteolysis and enzymatic cleavage with trypsin and endoproteinase Glu-C. The native mtSSB is composed of two related polypeptide chains, mtSSBs and mtSSBr. The sequence of mtSSBs consists of 129 amino acids with a calculated molecular mass of 14,627 Da. Comparison of the first 80 residues of the two chains reveals 91% identity. A high degree of similarity is found between mtSSB and Escherichia coli SSB or F sex factor SSB.
Ghrir
R
R
Institut de Génétique et Microbiologie, Université Paris-Sud, Orsay, France.
Lecaer
J P
JP
Dufresne
C
C
Gueride
M
M
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D008928:Mitochondria / Q000737:chemistry*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D012697:Serine Endopeptidases; D014357:Trypsin; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000235:genetics*
D000596:Amino Acids; D004268:DNA-Binding Proteins; D010446:Peptide Fragments; D029867:Xenopus Proteins; C070332:mtSSB-1 protein, Xenopus; D012697:Serine Endopeptidases; D002918:Chymotrypsin; C028344:glutamyl endopeptidase; D014357:Trypsin