title: Cardioactive neuropeptide Phe-Met-Arg-Phe-NH2 (FMRFamide) and novel related peptides are encoded in multiple copies by a single gene in the snail Lymnaea stagnalis.
abstract: The neuropeptide Phe-Met-Arg-Phe-NH2 (FMRFamide) is a potent cardioactive neuropeptide in Lymnaea stagnalis. Isolation and sequencing of 2 cDNAs and a genomic clone shows that a single gene encodes a precursor protein which contains 9 copies of the FMRFamide peptide, 2 copies of the related peptide Phe-Leu-Arg-Phe-NH2 (FLRFamide), and single copies of the putative pentapeptides Gln-Phe-Tyr-Arg-lle-NH2 (posttranslationally modified to pQFYRlamide) and Glu-Phe-Leu-Arg-lle-NH2 (EFLRlamide). The gene is transcribed in the CNS and gives rise to a single RNA of 1.7 kb in size. The organization of the Lymnaea gene is significant with respect to the evolution of FMRFamide and related peptides in other organisms.
authors.affiliation: Department of Biochemistry, School of Biological Sciences, Brighton, East Sussex, United Kingdom.
authors.forename: A
authors.initials: A
authors.lastname: Linacre
authors.forename: E
authors.initials: E
authors.lastname: Kellett
authors.forename: S
authors.initials: S
authors.lastname: Saunders
authors.forename: K
authors.initials: K
authors.lastname: Bright
authors.forename: P R
authors.initials: PR
authors.lastname: Benjamin
authors.forename: J F
authors.initials: JF
authors.lastname: Burke
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004247:DNA; D019835:FMRFamide; D005796:Genes; D006321:Heart / Q000187:drug effects*; D008195:Lymnaea / Q000235:genetics*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D018377:Neurotransmitter Agents; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D009479:Neuropeptides; D018377:Neurotransmitter Agents; D012333:RNA, Messenger; D019835:FMRFamide; D004247:DNA