Spectrophotometric determination of functional characteristics of protein kinases with coupled enzymatic assay.
The performance of enzyme-coupled spectrophotometric assay of protein kinases and its merits as compared with the radioisotopic method in characterizing functional activity of protein kinases are described. Kinetic parameters of protein kinase C and protein kinase A so obtained are presented. General application of the spectrophotometric assay in the study of protein kinases is recommended.
Technikova-Dobrova
Z
Z
Institute of Medical Biochemistry and Chemistry, University of Bari, Italy.
Sardanelli
A M
AM
Papa
S
S
D000818:Animals; D001921:Brain / Q000201:enzymology; D002853:Chromatography, Liquid; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011493:Protein Kinase C / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D013057:Spectrum Analysis
D011494:Protein Kinases; D011493:Protein Kinase C