title: Regional assignment of 41 human DNA fragments on chromosome 7 by means of a somatic cell hybrid panel.
abstract: To detect new restriction fragment length polymorphisms that would cover human chromosome 7 with a network of genetic landmarks, a chromosome 7-specific phage gene library was screened for human single-copy fragments. With use of a somatic cell hybrid panel containing defined regions of human chromosome 7, 41 cloned human single-copy sequences were assigned to five regions of this chromosome. Of special importance are the cell hybrid clones GM1059Rag5 and 7851Rag10-1, derived from human cells with interstitial deletions spanning the bands 7q22-q32, within which the cystic fibrosis gene is located. Twelve new probes are described in 7q22-q32, five of which detect a total of six RFLPs.
authors.affiliation: Institut für Humangenetik der Universität, Marburg, Federal Republic of Germany.
authors.forename: A
authors.initials: A
authors.lastname: Jobs
authors.forename: D
authors.initials: D
authors.lastname: Klein-Bölting
authors.forename: A S
authors.initials: AS
authors.lastname: Jandel
authors.forename: A
authors.initials: A
authors.lastname: Driesel
authors.forename: K
authors.initials: K
authors.lastname: Olek
authors.forename: K H
authors.initials: KH
authors.lastname: Grzeschik
mesh_terms: D000818:Animals; D002871:Chromosome Banding; D002897:Chromosomes, Human, Pair 7; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
chemical_list: D004247:DNA