title: Control of temperature-dependent synthesis of K99 fimbriae.
abstract: The influence of temperature on the production of K99 fimbriae by Escherichia coli was determined in cultures growing at constant specific growth rate in continuous cultures. In a wild type strain, in which the K99 operon is present on a low copy number plasmid, low cultivation temperature repressed the K99 production. This temperature-dependent production was not observed after introduction of multicopies of the regulatory region of the K99 operon into this strain, nor in E. coli K12 harbouring a recombinant, multicopy plasmid encoding the K99 operon. These results are in agreement with a regulation model in which a regulatory factor, most likely a repressor, inhibits expression of the K99 operon at low temperatures.
authors.affiliation: Department of Microbiology, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: M W
authors.initials: MW
authors.lastname: van der Woude
authors.forename: M
authors.initials: M
authors.lastname: Braster
authors.forename: H W
authors.initials: HW
authors.lastname: van Verseveld
authors.forename: F K
authors.initials: FK
authors.lastname: de Graaf
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009876:Operon; D010957:Plasmids; D013696:Temperature