title: Positive regulation of colonization factor antigen I (CFA/I) production by enterotoxigenic Escherichia coli producing the colonization factors CS5, CS6, CS7, CS17, PCFO9, PCFO159:H4 and PCFO166.
abstract: Entertoxigenic Escherichia coli (ETEC) strains of nineteen serogroups which produced colonization factors (coli-surface-associated antigens CS5, CS6, CS7 and CS17, colonization factor antigen CFA/III and putative colonization factors PCFO159:H4, PCFO166 and PCFO9) were tested for hybridization with a DNA probe containing the cfaD sequence that regulates expression of CFA/I. Strong colony hybridization, similar to that with the CFA/I-positive control strain H10407, occurred with ETEC strains of serogroups O27, O159 and O169 which produced CS6 antigen, and with all the strains which produced PCFO166 fimbriae. Weak colony hybridization, compared to the control strain, was found with ETEC producing CS5 fimbriae with CS6 antigen, CFA/III fimbriae with CS6 antigen, CS7 fimbriae or PCFO159:H4 fimbriae. CS6-antigen-positive strains of serogroups O79, O89 and O148 and all the CS17-antigen-positive and PCFO9-fimbriae-positive strains were negative in colony hybridization tests with the cfaD probe. Plasmid DNA of nine ETEC strains and their colonization-factor-negative derivatives was tested for hybridization with the cfaD probe and with ST and LT oligonucleotide probes. The sequences that hybridized with the cfaD probe were on the plasmids which coded for enterotoxin production. Fifteen strains were transformed with NTP513, a recombinant plasmid which contains the CFA/I region 1 fimbrial subunit operon but lacks a functional cfaD sequence, in order to determine whether DNA in any of these strains could substitute for the cfaD sequence in the regulation of production of CFA/I fimbriae.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Division of Enteric Pathogens, Central Public Health Laboratory, London, UK.
authors.forename: M L
authors.initials: ML
authors.lastname: Hibberd
authors.forename: M M
authors.initials: MM
authors.lastname: McConnell
authors.forename: G A
authors.initials: GA
authors.lastname: Willshaw
authors.forename: H R
authors.initials: HR
authors.lastname: Smith
authors.forename: B
authors.initials: B
authors.lastname: Rowe
mesh_terms: D000372:Agglutination Tests; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D015153:Blotting, Western; D015342:DNA Probes / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D036781:Fimbriae Proteins; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005809:Genes, Regulator / Q000235:genetics*; D006801:Humans; D008358:Mannose / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D009876:Operon / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D014169:Transformation, Bacterial
chemical_list: D000942:Antigens, Bacterial; D015342:DNA Probes; D004768:Enterotoxins; C022924:colonization factor antigens; D036781:Fimbriae Proteins; D008358:Mannose