Alterations in dynamics of microtubule assembly during axonal neuritogenesis in NB2a/d1 cells.
During dibutyryl cyclic AMP (dbcAMP)-mediated differentiation, axonal neurites elaborated by mouse NB2a/d1 neuroblastoma cells are initially colchicine-labile but attain colchicine-stability after 7 days. To examine whether or not differences in tubulin subunit turnover could account for the development of colchicine-stability, anti-tubulin antibodies were delivered into NB2a/d1 cells at various times during dbcAMP-mediated neurite outgrowth. These antibodies prevented initial neurite elaboration, and induced neurite retraction in cells treated with dbcAMP for up to 3 days, but did not induce neurite retraction for cells treated for 7 days. We conclude that a less dynamic, more slowly-turning over population of microtubules develops within neurites of cells treated with dbcAMP for 7 days.
Shea
T B
TB
Ralph Lowell Laboratories, McLean Hospital, Belmont, MA 02178.
Beermann
M L
ML
D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure*; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D008870:Microtubules / Q000378:metabolism*; D009447:Neuroblastoma / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
D003994:Bucladesine; D003078:Colchicine