title: Rapid preliminary differentiation of species within the Mycobacterium tuberculosis complex: proposition of a radiometric method.
abstract: A radiometric method using the "Bactec 460-TB" apparatus now enables rapid obtaining of drug-susceptibility data and differentiation of clinical mycobacterial specimens into those belonging to the Mycobacterium tuberculosis complex (comprised of M. tuberculosis, M. bovis, M. bovis BCG and M. africanum) and non-tuberculous mycobacteria, using a specific inhibitor (p-nitro-alpha-acetyl-amino-beta-hydroxypropiophenone). In the present work, further differentiation of the M. tuberculosis complex into 4 individual species was achieved using the susceptibility of strains to 1.25 micrograms/ml of thiophene-2-carboxylic acid hydrazide, 40 micrograms/ml of D-cycloserine, and 100 micrograms/ml of pyrazinamide.
authors.affiliation: Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, Paris.
authors.forename: K S
authors.initials: KS
authors.lastname: Goh
authors.forename: N
authors.initials: N
authors.lastname: Rastogi
mesh_terms: D003523:Cycloserine / Q000494:pharmacology; D006801:Humans; D006910:Hydroxypropiophenone / Q000031:analogs & derivatives / Q000494:pharmacology; D066298:In Vitro Techniques; D009161:Mycobacterium / Q000187:drug effects / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000302:isolation & purification*; D011718:Pyrazinamide / Q000494:pharmacology; D011874:Radiometry / Q000379:methods*; D013876:Thiophenes / Q000494:pharmacology; D014376:Tuberculosis / Q000382:microbiology*
chemical_list: D013876:Thiophenes; D006910:Hydroxypropiophenone; C016144:thiophene-2-carboxylic acid hydrazide; D011718:Pyrazinamide; C017561:4-nitro-alpha-acetylamino-beta-hydroxypropiophenone; D003523:Cycloserine