Restriction fragment length polymorphism in the cytadhesin P1 gene of human clinical isolates of Mycoplasma pneumoniae.
Genomic DNA obtained from Mycoplasma pneumoniae clinical isolates spanning a 30-year period was analyzed for the presence of polymorphism in their P1 cytadhesin genes. All clinical isolates expressed a 170-kilodalton P1 protein that reacted with anti-P1 monoclonal antibodies. However, Southern blot analysis of specific M. pneumoniae isolates with subclones of the P1 structural gene revealed the presence of restriction fragment length polymorphism, permitting the classification of their P1 genes into two distinct categories.
Dallo
S F
SF
Department of Microbiology, University of Texas Health Science Center at San Antonio.
Horton
J R
JR
Su
C J
CJ
Baseman
J B
JB
D018829:Adhesins, Bacterial; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics*; D015139:Blotting, Southern; D005798:Genes, Bacterial; D009177:Mycoplasma pneumoniae / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; C035471:adhesin, Mycoplasma pneumoniae