title: Primary standardization for the ELISA of serum thyroperoxidase and thyroglobulin antibodies and their prevalence in a normal Welsh population.
abstract: This paper describes the use of ELISA techniques, together with university available primary standards, for the assay of the thyroid autoantibodies anti-thyroperoxidase (TPOAb) and anti-thyroglobulin (TgAb). The data obtained using ELISA compared well with that obtained by passive haemagglutination giving correlation coefficients of 0.79 and 0.83 respectively. TPOAb and TgAb concentrations were assayed in serum samples from 832 healthy individuals aged between 18 and 45 years and cut-off values for both antibodies have been defined (TPOAb less than 19.6kIU/l; TgAb less than 98kIU/l). Applying these cut-off values, some 10% of the population showed elevated anti-thyroid antibody activity and, when divided by sex, a majority of this group belonged to the female population.
authors.affiliation: Department of Medicine, Cardiff Institute for Higher Education, Wales.
authors.forename: C J
authors.initials: CJ
authors.lastname: Groves
authors.forename: R D
authors.initials: RD
authors.lastname: Howells
authors.forename: S
authors.initials: S
authors.lastname: Williams
authors.forename: C
authors.initials: C
authors.lastname: Darke
authors.forename: A B
authors.initials: AB
authors.lastname: Parkes
mesh_terms: D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D005260:Female; D006386:Hemagglutination Tests / Q000592:standards; D006801:Humans; D007453:Iodide Peroxidase / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012015:Reference Standards; D012680:Sensitivity and Specificity; D012727:Sex Characteristics; D013954:Thyroglobulin / Q000276:immunology*; D013967:Thyroiditis, Autoimmune / Q000453:epidemiology*; D014852:Wales / Q000453:epidemiology
chemical_list: D001323:Autoantibodies; D013954:Thyroglobulin; D007453:Iodide Peroxidase