Stage specific phosphoinositides turnover capacity of human intrathymic T cells following CD2-triggering.
Triggering of distinct CD2 epitopes on human T lymphocytes increases their phosphatidylinositol (PI) cycle-related metabolism. In this work, we investigated the relationship between this signal transduction pathway following surface CD2 antigen triggering and intrathymic T cell development. Therefore, various thymocyte subsets were incubated with co-mitogenic CD2I+III mAb. The cells were then tested for their various phosphoinositides levels as well as their ability to proliferate in response to recombinant interleukin-2 (rIL-2). Our results indicate that immature CD4- CD8- cells have high PI metabolism while more mature CD4+CD8+ and unfractionated thymocytes display significantly lower PI-turnover. Mature CD4+CD8- and CD4-CD8+ thymocytes regain this transduction capacity. Thus, PI-turnover following CD2- triggering is linked to the developmental fate of thymocyte subclasses.
Mossalayi
M D
MD
Laboratoire d'Immunologie Cellulaire, CNRS URA-186, Hôpital Pitié-Salpêtrière, Paris, France.
Dalloul
A H
AH
Bertho
J M
JM
Bismuth
G
G
Blanc
C
C
Debré
P
P
D015703:Antigens, CD / Q000494:pharmacology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000494:pharmacology*; D018801:CD2 Antigens; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D007376:Interleukin-2; D010716:Phosphatidylinositols; D011971:Receptors, Immunologic