Effects of tobacco smoke on respiratory epithelial clearance of DTPA and on lung histology in rats.
The respiratory epithelial clearance of 99mTc-(DTPA) diethylenetriamine penta-acetate (RC-DTPA) was measured in rats before and after sham or cigarette smoke exposures. RC-DTPA was increased by cigarette smoke exposure (p less than 0.001); the amount and duration of the exposure had no significant effect. In smoker animals, the number of bronchoalveolar macrophages was decreased after one or five smoke exposures a day for one day (p less than 0.001) and the number of bronchoalveolar neutrophils was increased after five exposures a day for ten days (p less than 0.001). No abnormality was found in the lung parenchyma on light microscopy. We conclude that in rats RC-DTPA is increased by exposure to tobacco smoke, whatever the amount and duration of exposure; however, no gross parenchymal lung abnormality explains the increase in RC-DTPA.
Mordelet-Dambrine
M
M
Université René Descartes, Hôpital Ambroise Paré, Boulogne, France.
Leguern-Stanislas
G
G
Chinet
T C
TC
Barritault
L
L
Chrétien
J
J
Huchon
G J
GJ
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004848:Epithelium / Q000378:metabolism; D008168:Lung / Q000033:anatomy & histology / Q000378:metabolism*; D008264:Macrophages; D008297:Male; D009504:Neutrophils; D004369:Pentetic Acid / Q000493:pharmacokinetics*; D010539:Permeability; D010947:Plants, Toxic; D051381:Rats; D011919:Rats, Inbred Strains; D012906:Smoke; D014026:Nicotiana
D012906:Smoke; D004369:Pentetic Acid