Humoral immune response to Bacteroides gingivalis fimbrial antigen in mice.
Bacteroides gingivalis fimbrial antigen incorporated into liposomes, but not in Tris-HCl buffer, significantly raised the levels of anti-fimbriae antibodies in serum, particularly of the IgG class, after oral primary and booster immunizations in BALB/c mice. An approximately linear relationship was observed between the dose of fimbrial antigen and the level of fimbriae-specific antibodies produced; antibody production reached its maximum at an immunization dosage of 500 micrograms of fimbriae per mouse. Fimbriae-specific antibody production was enhanced by use of a semi-synthetic adjuvant, a stearoyl derivative of sodium beta-N-acetylglucosaminyl-(1----4)-N-acetylmuramyl-L-alanyl-D-isoglutaminyl-(L) - stearoyl-(D)-meso-diamino-pimelic acid-(D)-amide-D-alanine (GM)-53) in liposomes. High anti-fimbriae antibody levels in serum and saliva were maintained for several months in the mice that had received two orally administered boosters of fimbrial antigen with GM-53 in liposomes. Salivary anti-fimbriae antibody levels, particularly of the IgA class, were markedly raised.
Ogawa
T
T
Department of Oral Microbiology, Osaka University Faculty of Dentistry, Japan.
Shimauchi
H
H
Kusumoto
Y
Y
Hamada
S
S
D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D001439:Bacteroides / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D010861:Fimbriae, Bacterial / Q000276:immunology*; D007700:Kinetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial