title: Bovine rhodopsin: amino acid substitutions Asp-83----Asn and Glu-134----Gln prevent activation of cyclic GMP phosphodiesterase.
abstract: Two bovine rhodopsin mutants with substitutions of negatively charged residues within transmembrane domains II and III by uncharged ones (Asp-83----Asn and Glu-134----Gln) were constructed. Both mutants stimulated transducin GTPase with slightly lowered efficiency, but were completely unable to activate cyclic GMP phosphodiesterase.
authors.affiliation: Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow, Region.
authors.forename: V V
authors.initials: VV
authors.lastname: Gurevich
authors.forename: S A
authors.initials: SA
authors.lastname: Zozulya
authors.forename: E P
authors.initials: EP
authors.lastname: Zerf
authors.forename: I D
authors.initials: ID
authors.lastname: Pokrovskaya
authors.forename: T A
authors.initials: TA
authors.lastname: Obukhova
authors.forename: M N
authors.initials: MN
authors.lastname: Garnovskaya
authors.forename: I L
authors.initials: IL
authors.lastname: Dumler
authors.forename: M P
authors.initials: MP
authors.lastname: Rychkova
mesh_terms: D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004247:DNA / Q000235:genetics; D004789:Enzyme Activation / Q000187:drug effects; D020558:GTP Phosphohydrolases / Q000378:metabolism; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D012243:Rhodopsin / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology
chemical_list: D004247:DNA; D012243:Rhodopsin; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D020558:GTP Phosphohydrolases; C072504:transducin GTP phosphohydrolase