Assessment of cellular activation by flow cytometric methods.
The in vitro measurement of lymphoid cell activation is a valid correlate of immunological function in human subjects. This process can be evaluated by the assessment of the proliferative response or the cytokine synthesis and secretion by lymphoid cells upon their proper stimulation. The technology of flow cytometry provides unique conditions for the development of biological assays to achieve those purposes. In this work we describe the utilization of flow cytometric DNA analysis in the evaluation of: (1) the mitogenic response of lymphoid cells; (2) the cellular activity in mixed lymphocyte cultures; (3) the presence of interleukin-2 in culture supernatants, and (4) the cell cycle progression, simultaneous to the expression of a nuclear activation antigen among proliferating lymphocytes.
Lavergne
J A
JA
School of Medicine, University of Puerto Rico, Río Piedras.
del Llano
A M
AM
D000818:Animals; D000911:Antibodies, Monoclonal; D002453:Cell Cycle; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000032:analysis; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000166:cytology*; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D018809:Proliferating Cell Nuclear Antigen
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D009687:Nuclear Proteins; D010835:Phytohemagglutinins; D018809:Proliferating Cell Nuclear Antigen