[Immunological screening of clonorchiasis].
Crude antigens of Clonorchis sinensis adult worms were purified by Sephadex G-200 column chromatography. A purified antigen was then applied to detect clonorchiasis patients and parasite-free subjects by an enzyme-linked immunosorbent assay (ELISA) and an intradermal test (IDT). The results of the ELISA showed that specific antibody levels of IgG, IgM, IgA, IgE and total IgE in 371 infected patients were significantly higher than those of 181 parasite-free subjects (Student's t-test, p less than 10(-6) or p less than 0.005). The sensitivity was 92.8% and the specificity was 89.0%. Significant correlations between the intensity of infection, expressed as egg per gram feces, and specific IgG, IgA and total IgE concentration were observed (linear regression, p less than 0.0005). Among the 309 subjects tested intradermally with Clonorchis antigen, 128 showed a positive reaction while all of the 60 parasite-free subjects showed negative results. By this test, the sensitivity was 49% and the specificity of IDT was 100%. The specific IgG, IgA and total IgE antibody levels of the IDT positive patients were significantly higher than those of the negative subjects (Student's t-test, p less than 0.0005). There was no correlation between the induced swelling diameter and the intensity of infection in IDT positive clonorchiasis patients. Cross reactions in ELISA were observed in patients infected with Capillaria philippinensis, Sparganum proliferum and Toxocara canis, when the antigens prepared in the present study were used.
Lin
Y L
YL
Department of Parasitology, Chang Gung Medical College.
Yen
C M
CM
Chen
E R
ER
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D003003:Clonorchiasis / Q000175:diagnosis* / Q000276:immunology; D003004:Clonorchis sinensis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007428:Intradermal Tests; D011237:Predictive Value of Tests; D012044:Regression Analysis
D000909:Antibodies, Helminth; D007136:Immunoglobulins