Passive protection against infectious bursal disease virus by viral VP2 expressed in yeast.
Infectious bursal disease virus (IBDV), a pathogen of major economic importance to the world's poultry industries, causes a severe immunodepressive disease in young chickens. Maternal antibodies are able to protect the progeny passively from IBDV infection. The gene encoding the IBDV host-protective antigen (VP2) has been cloned and expressed in yeast resulting in the production of an antigen that very closely resembles native VP2. When injected into specific pathogen free chickens a single dose of microgram quantities of the yeast derived antigen induces high titres of virus neutralizing antibodies that are capable of passively protecting young chickens from infection with IBDV.
Macreadie
I G
IG
Division of Biomolecular Engineering, Royal Parade, Parkville, Australia.
Vaughan
P R
PR
Chapman
A J
AJ
McKern
N M
NM
Jagadish
M N
MN
Heine
H G
HG
Ward
C W
CW
Fahey
K J
KJ
Azad
A A
AA
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D007106:Immune Sera / Q000276:immunology; D007116:Immunization, Passive; D007243:Infectious bursal disease virus / Q000276:immunology*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D007106:Immune Sera