[Characterization of oncogenes (K, N-ras and N, c-myc) in subcloned cell lines (KCC-1a and 1b) derived from same endometrial carcinoma presenting well differentiated adenocarcinoma and clear cell carcinoma].
Human endometrial adenocarcinoma cell lines (KCC-1a and KCC-1b) were established from the same endometrial carcinoma. 1) Amplification of the N-ras gene was not able to be observed in Southern blot hybridization. K-ras gene amplification of KCC-1b was more than 10 fold that of KCC-1a and the control. N-myc gene amplification of KCC-1b was more than 3-5 fold that of KCC-1a. c-myc gene amplifications of KCC-1a and KCC-1b were more than 5-7 fold that of the control DNA. 2) p21 was observed in tubular adenocarcinoma but not in clear cell carcinoma. p21 was detected in KCC-1b cells but not in KCC-1a cells. 3) KCC-1a and KCC-1b cells had quite different characteristics in molecular biology.
Kataoka
A
A
Department of Obstetrics and Gynecology, School of Medicine, Kurume University.
Yakushiji
M
M
Ohbuchi
T
T
Morimitsu
Y
Y
Kojiro
M
M
D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism; D015139:Blotting, Southern; D002999:Clone Cells; D005260:Female; D005784:Gene Amplification; D016259:Genes, myc; D011905:Genes, ras; D006801:Humans; D016283:Proto-Oncogene Proteins p21(ras) / Q000032:analysis; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000235:genetics* / Q000378:metabolism
C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)