Apamin binding sites in human C6 cell line and their accessibility to monoclonal antibodies.
Apamin, a 18-amino acid bee venom toxic peptide specifically blocks a class of Ca(2+)-activated K+ channels. i) Mono 125I-iodoapamin binds to receptor sites in a human neuroglial cell line (C6 line) but not in human cell lines from pancreatic (RIN5F line) and colonic origin (HT29 line). ii) Receptor-bound apamin is still accessible to a large molecule since some anti-apamin monoclonal antibodies recognize apamin when bound to its receptor, both in intact cells of the human C6 glioma line and in rat brain synaptosomal membranes.
Defendini
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ML
Laboratoire de Biochimie, Centre National de la Recherche Scientifique, Faculté de Médecine, Marseille, France.
Juin
M
M
Granier
C
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001030:Apamin / Q000378:metabolism*; D001665:Binding Sites; D002448:Cell Adhesion; D002460:Cell Line; D005910:Glioma / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008566:Membranes / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D015221:Potassium Channels; D051381:Rats; D011919:Rats, Inbred Strains; D017981:Receptors, Neurotransmitter / Q000276:immunology / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D015221:Potassium Channels; D017981:Receptors, Neurotransmitter; C033799:apamin receptor; D001030:Apamin