[Increased in vitro motility of human vascular wall myocytes from restenotic lesions of peripheral and coronary vessels].
In this study we report on the successful cultivation of human peripheral and coronary plaque specimens selectively retrieved by percutaneous Simpson atherectomy and obtained by direct operative approach. A total of 32 patients in whom plaque tissue was excised from 22 primary and 10 restenotic lesions comprise the study population. Irrespective of their origin or location, all advanced lesions showed smooth muscle cells (SMC) to be their predominant cell type proven by indirect immunofluorescence technique. Cultured endothelial cells were only identified in 2/6 surgically removed samples. Locomotion analysis of cultured smooth muscle cells was performed with a standardized computer-assisted video system. Cells of all groups exhibited random motility. However, SMC migratory velocity of restenotic origin amounted to 47.4 +/- 3.4 microns/h (n = 10, x +/- SD) and thereby was found 2.4 times (p less than 0.001) increased as compared to primary lesion values of 22.0 +/- 3.7 microns/h (n = 22, x +/- SD). This highly significant difference was seen for both peripheral and coronary lesions. Our data suggest increased SMC migratory activity to represent a basic biological mechanism involved in human accelerated arteriosclerosis and restenosis formation.
Bauriedel
G
G
Medizinische Klinik I, Ludwig-Maximilians-Universität München.
Windstetter
U
U
Brandl
R
R
Plas
E
E
Kandolf
R
R
Höfling
B
B
D000368:Aged; D001161:Arteriosclerosis / Q000473:pathology* / Q000601:surgery; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D003324:Coronary Artery Disease / Q000473:pathology* / Q000601:surgery; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000473:pathology*; D012008:Recurrence
Erhöhte In-vitro-Motilität humaner Gefässwandmyozyten aus Restenose-Läsionen peripherer und koronarer Gefässe.