title: Detection of staphylococcal enterotoxin and toxic shock syndrome toxin-1 (TSST-1) by immunoblot combined with a semiautomated electrophoresis system.
abstract: An immunoblot technique combined with a semi-automated electrophoresis system has been developed for the detection of staphylococcal enterotoxins and toxic shock syndrome toxin-1 (TSST-1). The advantages of this method over other detection techniques include speed, sensitivity (10 ng/ml) and specificity. The use of semiautomated electrophoresis permits the routine detection of staphylococcal enterotoxins and TSST-1.
authors.affiliation: Dpto. Patología Animal I, Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Orden
authors.forename: J
authors.initials: J
authors.lastname: Goyache
authors.forename: F J
authors.initials: FJ
authors.lastname: Hernández
authors.forename: A
authors.initials: A
authors.lastname: Doménech
authors.forename: G
authors.initials: G
authors.lastname: Suárez
authors.forename: E
authors.initials: E
authors.lastname: Gómez-Lucía
mesh_terms: D001427:Bacterial Toxins; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D008970:Molecular Weight; D013211:Staphylococcus aureus; D018089:Superantigens
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal