Survival and in vitro fertilization potential of sperm following washing and incubation with different protein supplements.
Three protein supplements, preovulatory donor serum (PDS), Cohn Fr V human serum albumin (HSA), and highly purified HSA were used for washing sperm. The ability of the different protein supplements to support sperm survival and in vitro fertilization of zona-free hamster oocytes was compared following 18 hours of incubation. Sperm motility, velocity, linearity, and motility index were the same for the various proteins. The percentage of sperm with a functional plasma membrane was significantly greater (P less than .02) for highly purified HSA than for PDS or Cohn Fr V HSA. The percentage of zona-free hamster oocytes penetrated and penetrations per oocyte were two times greater (P less than .0001) for PDS and Cohn Fr V HSA compared with highly purified HSA. These data indicate that (1) proteins can possess an equal ability to support sperm survival, yet differ in their ability to support capacitation and the acrosome reaction; (2) HSA preparations derived from dissimilar purification methods may differ in their ability to support in vitro fertilization; (3) PDS and certain types of HSA possess an equal ability to support in vitro fertilization.
Hammitt
D G
DG
Department of Obstetrics and Gynecology, University of Iowa Hospitals and Clinics, Iowa City.
Walker
D L
DL
Williamson
R A
RA
D001798:Blood Proteins; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008297:Male; D010060:Ovulation; D012709:Serum Albumin / Q000494:pharmacology*; D013075:Sperm Capacitation / Q000187:drug effects; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa
D001798:Blood Proteins; D012709:Serum Albumin