Isolation of DNA insertion elements from Rhizobium meliloti which are able to promote transcription of adjacent genes.
In order to select insertion sequences able to promote transcription of flanking genes (ISp elements), three mobilizable RSF1010 derived vectors were constructed. Using promoterless antibiotic resistance genes, ISp elements ranging from 0.75 to 2.9 kb were isolated from Escherichia coli and Rhizobium meliloti. Restriction and hybridization experiments revealed that identical ISp elements could be isolated from different R. meliloti strains and that one of these is similar to an insertion sequence found previously in R. meliloti 2011.
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Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, Germany.
Simon
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D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D005822:Genetic Vectors; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015183:Restriction Mapping; D012231:Rhizobium / Q000235:genetics*; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D004269:DNA, Bacterial