Genotypic analyses of Hodgkin's disease.
In spite of the use of molecular biology, the cellular lineage and clonality of Reed-Sternberg cells, the abnormal cells of Hodgkin's disease, remain an enigma. We studied the pattern of rearrangements at immunoglobulin and T-cell receptor loci in 23 patients suffering from Hodgkin's disease. Two out of 23 patients exhibited immunoglobulin gene rearrangements. No rearrangements of the T-cell receptor beta-chain gene were detected in any patient examined. Our results showed no correlation between the presence of rearranged bands and the number of Reed-Sternberg cells.
Bernard
D J
DJ
Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, Clermont-Ferrand, France.
Bignon
Y J
YJ
Fonck
Y
Y
Ramos
F
F
Chassagne
J
J
Chollet
P
P
Plagne
R
R
D015703:Antigens, CD / Q000032:analysis* / Q000235:genetics; D015139:Blotting, Southern; D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D005787:Gene Frequency / Q000235:genetics; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D005838:Genotype; D005854:Germ Cells / Q000737:chemistry; D006689:Hodgkin Disease / Q000235:genetics*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000032:analysis*; D007150:Immunohistochemistry; D008198:Lymph Nodes; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell / Q000032:analysis*
D015703:Antigens, CD; D015342:DNA Probes; D007143:Immunoglobulin Heavy Chains; D011948:Receptors, Antigen, T-Cell; D004247:DNA