Regional assignment of 41 human DNA fragments on chromosome 7 by means of a somatic cell hybrid panel.
To detect new restriction fragment length polymorphisms that would cover human chromosome 7 with a network of genetic landmarks, a chromosome 7-specific phage gene library was screened for human single-copy fragments. With use of a somatic cell hybrid panel containing defined regions of human chromosome 7, 41 cloned human single-copy sequences were assigned to five regions of this chromosome. Of special importance are the cell hybrid clones GM1059Rag5 and 7851Rag10-1, derived from human cells with interstitial deletions spanning the bands 7q22-q32, within which the cystic fibrosis gene is located. Twelve new probes are described in 7q22-q32, five of which detect a total of six RFLPs.
Jobs
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Institut für Humangenetik der Universität, Marburg, Federal Republic of Germany.
Klein-Bölting
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Jandel
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Driesel
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A
Olek
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Grzeschik
K H
KH
D000818:Animals; D002871:Chromosome Banding; D002897:Chromosomes, Human, Pair 7; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D004247:DNA