[Determination of beta-N-acetylglucosaminidase and albumin in urine: comparison of methods and effect of gel filtration in human and rat urine].
A new kinetic method for determination of beta-N-acetylglucosaminidase (beta-NAG, EC 3.2.1.30) in human and rat urine with chlorophenol red-NAG as substrate is compared to two other methods with 4-nitrophenyl-NAG and 3-cresol-sulfonphthaleinyl-NAG respectively as substrates. The impact of gel filtration of urinary specimens on the determination of beta-NAG-activity and of low albumin concentrations in urine is described.
Meier
M
M
Praxis für Laboratoriumsmedizin, Kiel, Bundesrepublik Deutschland.
Wilke
B
B
Junge
W
W
D000118:Acetylglucosaminidase / Q000652:urine*; D000419:Albuminuria / Q000175:diagnosis* / Q000201:enzymology*; D000818:Animals; D002850:Chromatography, Gel; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007677:Kidney Function Tests / Q000379:methods*; D009391:Nephelometry and Turbidimetry / Q000379:methods; D010637:Phenolsulfonphthalein / Q000031:analogs & derivatives; D051381:Rats; D011919:Rats, Inbred Strains
C007016:chlorophenol red; D000118:Acetylglucosaminidase; D010637:Phenolsulfonphthalein
Bestimmung von beta-N-Acetylglucosaminidase und Albumin im Urin: Methodenvergleiche sowie Einfluss der Gelfiltration in human- und Rattenurinen.