Effects of carbodiimide structure on the immobilization of enzymes.
A series of water-soluble disubstituted carbodiimides of different structure was tested for enzyme immobilization. In the experiments, a polyacrylamide-type bead polymer possessing carboxylic functional groups was used as support. The enzymes immobilized were aminoacylase (N-acylamino acid amidohydrolase; EC 3.5.1.14), arginase (L-arginine amidinohydrolase; EC 3.5.3.1), cyclodextrin glycosyltransferase (alpha-1,4-glucan 4-glycosyltransferase, cyclizing; EC 3.2.1.19), glucoamylase (1,4-alpha-D-glucan glycohydrolase, EC 3.2.1.3), and carboxypeptidase B (peptidyl-L-lysine [L-arginine] hydrolase; EC 3.4.17.2). It was found that the degree of immobilization strongly depended on the structure of carbodiimide used.
Szajáni
B
B
Reanal Factory of Laboratory Chemicals, Budapest, Hungary.
Südi
P
P
Klamár
G
G
Jászay
Z M
ZM
Petneházy
I
I
Töke
L
L
D001709:Biotechnology; D002234:Carbodiimides / Q000737:chemistry*; D004800:Enzymes, Immobilized; D015394:Molecular Structure
D002234:Carbodiimides; D004800:Enzymes, Immobilized