1H NMR of 5'CGCGTATATACGCG3', a duplex and a four-membered loop.
The deoxyribonucleotide 5'CGCGTATATACGCG3' was synthesized and studied by NMR methods. This short, fully palindromic duplex also forms a hairpin under certain conditions described within. As such, it is considered to be a model for cruciform formation. We show that this sequence forms a four-membered loop and a duplex in solution. The duplex is shown to be a normal, B-DNA like helix, while the hairpin is shown to be a four-membered ATAT loop.
Kallick
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DA
Department of Chemistry, University of California, Berkeley.
Wemmer
D E
DE
D001483:Base Sequence; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D013696:Temperature
D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides