title: Comparison of direct immunoperoxidase and direct immunofluorescence for the detection of herpes simplex virus antigen in cell culture.
abstract: 150 specimens from suspected herpes simplex genital and skin lesions were received in virus transport medium. They were inoculated into Hep-2 cell monolayers, examined for the presence of virus by cytopathic effect (CPE), direct immunoperoxidase (DIP) and direct immunofluorescence (DIF). Of 39 (26.0%) virus-positive specimens by CPE, 37 (24.7%) were HSV-positive by DIP and 36 (24.0%) by DIF staining. DIP staining had a sensitivity of 100%, specificity of 99.1%, positive predictive value of 97.3% and negative predictive value of 100% in relation to DIF as a standard test. Of 39 specimens positive by CPE, only 25.6% were HSV-positive within 24 h post-inoculation compared to 94% HSV-positive by DIP and DIF staining at the same time.
authors.affiliation: Department of Microbiology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur.
authors.forename: D
authors.initials: D
authors.lastname: Sabil
authors.forename: S K
authors.initials: SK
authors.lastname: Othman
authors.forename: I
authors.initials: I
authors.lastname: Isahak
mesh_terms: D000956:Antigens, Viral / Q000032:analysis*; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D018139:Simplexvirus / Q000276:immunology*
chemical_list: D000956:Antigens, Viral