Serum manganese-superoxide dismutase in patients with neuromuscular disorders as judged by an ELISA.
Manganese-superoxide dismutase (Mn-SOD) concentration was measured in sera from 37 healthy controls and 101 patients with 11 forms of neuromuscular diseases including Duchenne muscular dystrophy (DMD) and polymyositis (PMS) by an enzyme-linked immunosorbent assay with the use of a monoclonal antibody against human liver Mn-SOD. Serum from patients with DMD had a significantly (P less than 0.05) lower concentration of Mn-SOD than control serum. On the other hand, the concentration of Mn-SOD was markedly higher in the serum of patients with untreated form of acute PMS. The enzyme appeared to provide a good index for monitoring of responses to treatment of acute PMS. Of other neuromuscular diseases Mn-SOD concentration decreased significantly (P less than 0.05) in Charcot-Marie-Tooth disease and Kennedy-Alter-Sung syndrome but increased significantly (P less than 0.05) in human T-cell lymphotrophic viruses-I-associated myelopathy. This enzyme profile seems to be specific to each neuromuscular disease.
Yahara
O
O
Department of Internal Medicine, Asahikawa Medical College, Japan.
Hashimoto
K
K
Taniguchi
N
N
Ishikawa
M
M
Sato
Y
Y
Yamashita
H
H
Ohno
H
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D002607:Charcot-Marie-Tooth Disease / Q000201:enzymology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015490:HTLV-I Infections / Q000201:enzymology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009136:Muscular Dystrophies / Q000201:enzymology*; D009220:Myositis / Q000201:enzymology; D009468:Neuromuscular Diseases / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000097:blood*
D000911:Antibodies, Monoclonal; D013482:Superoxide Dismutase