Synthesis of a monoclonal antibody-indium-111-porphyrin conjugate.
Antibodies were labelled with indium-111 with a view to their use in the radio-immunodetection of cancers. The covalent coupling between indium-111 porphyrin and monoclonal antibodies (IgG and F(ab')2 fragment) was achieved using the ester activated method [N-hydroxy-succinimide/1-ethyl-3-(3-dimethylaminopropyl)carbodiimide]. After purification, this provided conjugated with specific activities of 6 muCi/micrograms Mab (9.3 molecules per Mab) or 1 muCi/microgram (F(ab')2 fragment (1.5 molecule per F(ab')2). ELISA procedures suggested the full retention of immunoreactivity by the radiolabelled antibodies.
Bedel-Cloutour
C H
CH
Laboratoire de Chimie Organique et Organométallique, URA 35 CNRS, Université Bordeaux I, Talence, France.
Maneta-Peyret
L
L
Pereyre
M
M
Bezian
J H
JH
D000818:Animals; D000911:Antibodies, Monoclonal; D006801:Humans; D007205:Indium Radioisotopes; D007553:Isotope Labeling; D051379:Mice; D011166:Porphyrins; D012710:Serum Albumin, Bovine
D000911:Antibodies, Monoclonal; D007205:Indium Radioisotopes; D011166:Porphyrins; D012710:Serum Albumin, Bovine