Effect of maitotoxin on calcium current and background inward current in isolated ventricular rat myocytes.
Maitotoxin (MTX) irreversibly suppressed the voltage-dependent calcium current after a variable delay, an effect which was preceded, in 61% of the cells, by a transient increase in calcium current partly attributable to a shift (4-7 mV) of the activation curve towards negative potentials. MTX also induced the development of a voltage-independent background inward current which did not occur in the absence of external calcium and was reduced by removal of external sodium, by calcium channel blockers and by high concentrations of quinidine. MTX-induced single channel activity consisted of long lasting bursts of inward current. Channel activity was voltage-independent, with a unitary conductance of 14 pS and an extrapolated reversal potential of +16 mV. Single-channel current amplitude was not detectably reduced in the absence of external calcium but strongly reduced in the absence of external sodium, in the presence of 2 mM nickel or when external sodium was replaced by 96 mM calcium or 50 mM barium. The channel activity was also inhibited by quinidine. It is concluded that MTX alters, then suppresses the voltage-activated calcium current and induces the development of a voltage-independent inward current, part of which results from the opening of nickel-sensitive cation channels, mostly permeable to sodium ions.
Faivre
J F
JF
Laboratoire de Physiologie comparée (URA CNRS 1121), Université ParisXI, Orsay, France.
Deroubaix
E
E
Coulombe
A
A
Legrand
A M
AM
Coraboeuf
E
E
D000818:Animals; D015220:Calcium Channels / Q000187:drug effects*; D066298:In Vitro Techniques; D008387:Marine Toxins / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D009532:Nickel / Q000494:pharmacology; D035964:Oxocins; D011802:Quinidine / Q000494:pharmacology; D051381:Rats; D015222:Sodium Channels / Q000187:drug effects; D000095702:Polyether Toxins
D015220:Calcium Channels; D008387:Marine Toxins; D009532:Nickel; D035964:Oxocins; D011802:Quinidine; D015222:Sodium Channels; C020394:maitotoxin; D000095702:Polyether Toxins