Comparison between the polymerase chain reaction and slot blot hybridization for the detection of HPV sequences in cervical scrapes.
Sixty-four women presenting with a single mildly abnormal smear were investigated for infection with human papillomavirus (HPV) type 16 using both slot blot hybridization and polymerase chain reaction (PCR) amplification. PCR was nearly three times more sensitive for the detection of HPV 16 DNA than slot blot hybridization. HPV 16 was not significantly associated with a risk of progression to CIN if PCR was used to screen for infection. Women who smoked were at significantly greater risk of progression to CIN than non-smokers.
Ward
P
P
Department of Cytopathology, St Mary's Hospital Medical School, London.
Parry
G N
GN
Yule
R
R
Coleman
D V
DV
Malcolm
A D
AD
D000328:Adult; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012907:Smoking / Q000009:adverse effects; D014626:Vaginal Smears
D004279:DNA, Viral