Use of monoclonal antibody against major internal protein p24 of bovine leukemia virus in capture ELISA.
A monoclonal antibody (4H4) against the major internal protein, p24, of bovine leukemia virus (BLV) is described. It recognizes a sequence determinant on the p24-molecule and displays high affinity to its antigen. The monoclonal antibody 4H4 was applied in capture ELISA for diagnosis of enzootic bovine leukosis, using crude BLV-preparation as antigen. This test is more sensitive than the immunodiffusion test and at least as sensitive as direct ELISA.
Platzer
C
C
Central Institute of Molecular Biology, Academy of Sciences, Berlin, Buch.
Siakkou
H
H
Kraus
G
G
Gröbel
C
C
Rosenthal
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D007938:Leukemia / Q000175:diagnosis / Q000662:veterinary*; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D011237:Predictive Value of Tests; D014764:Viral Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D014764:Viral Proteins