Rabbit serum amyloid a gene: cloning, characterization and sequence analysis.
A genomic DNA clone that encodes serum amyloid A protein has been isolated from a rabbit liver lambda EMBL3 genomic library. Southern blot analysis indicates that the cloned gene is a member of multiple SAA gene family in rabbit. The nucleotide sequence of this DNA shows that the cloned gene corresponds to a SAA cDNA recently isolated from an acute phase rabbit liver cDNA library (1). This rabbit SAA gene contains four exons which are located within a 4500-base pair region spanning a putative TATA box and an AATAAA polyadenylation signal. DNA sequence upstream of the major transcription start site also contains other structural elements such as the sequences for NF-kappa B, IL-6 receptor and Sp1 binding. Significance of these regulatory elements in SAA gene expression is discussed.
Ray
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BK
Department of Veterinary Microbiology, College of Veterinary Medicine, University of Missouri, Columbia 65211.
Ray
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D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D005796:Genes; D015698:Genomic Library; D008099:Liver / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family; D009838:Oligodeoxyribonucleotides; D011817:Rabbits; D015183:Restriction Mapping; D000685:Serum Amyloid A Protein / Q000235:genetics*; D014158:Transcription, Genetic
D009838:Oligodeoxyribonucleotides; D000685:Serum Amyloid A Protein; D004247:DNA