title: B cells are required for induction of T cell abnormalities in a murine retrovirus-induced immunodeficiency syndrome.
abstract: The role of B cells in induction of phenotypic and functional abnormalities of T cells in a murine retrovirus-induced immunodeficiency syndrome, MAIDS, was evaluated in mice depleted of mature B cells from birth with anti-IgM antibodies (mu-suppressed) and infected at 4 wk of age. Multicolor FACS analyses of CD4+ T cell subsets showed that development of phenotypic abnormalities of these cells at 9 wk after infection was completely inhibited by mu-suppression. Furthermore, induction of impaired proliferative responses to Con A and alloantigens and CTL responses to alloantigens was fully blocked in antibody-treated animals. The extent of virus replication was comparable in spleens of untreated and mu-suppressed mice. Retroviral induction of T cell dysfunction in MAIDS is thus dependent on the presence of B cells, and high level virus expression in mice without B cells has little or no effect on T cell function.
authors.affiliation: Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
authors.forename: A
authors.initials: A
authors.lastname: Cerny
authors.forename: A W
authors.initials: AW
authors.lastname: Hügin
authors.forename: R R
authors.initials: RR
authors.lastname: Hardy
authors.forename: K
authors.initials: K
authors.lastname: Hayakawa
authors.forename: R M
authors.initials: RM
authors.lastname: Zinkernagel
authors.forename: M
authors.initials: M
authors.lastname: Makino
authors.forename: H C
authors.initials: HC
authors.lastname: Morse
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D015704:CD4 Antigens / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D003208:Concanavalin A; D004797:Enzyme-Linked Immunosorbent Assay; D007075:Immunoglobulin M / Q000032:analysis; D007153:Immunologic Deficiency Syndromes / Q000276:immunology* / Q000382:microbiology; D009052:Leukemia Virus, Murine; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014412:Tumor Virus Infections / Q000382:microbiology*
chemical_list: D015704:CD4 Antigens; D007075:Immunoglobulin M; D003208:Concanavalin A