Thrombospondin in ligament, meniscus and intervertebral disc.
The presence of thrombospondin in extracts of ligament, meniscus and human nucleus pulposus was demonstrated by Western blot analysis using an anti-human thrombospondin antibody. Metabolic labeling of ligament fibroblast and meniscal fibrochondrocyte cultures followed by immunoprecipitation with anti-human thrombospondin revealed a single band corresponding to the molecular weight of thrombospondin when analyzed by SDS-PAGE and fluorography under reduced and non-reduced conditions. Thrombospondin, therefore, is a matrix constituent of ligament, knee joint meniscus and the nucleus pulposus and is synthesized by ligament fibroblasts and meniscal fibrochondrocytes in vitro.
Miller
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Department of Musculoskeletal Research, Cleveland Clinic Foundation Research Institute, OH 44195-5254.
McDevitt
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CA
D000818:Animals; D000843:Ankle Joint; D015153:Blotting, Western; D002358:Cartilage, Articular / Q000737:chemistry; D002417:Cattle; D004285:Dogs; D005347:Fibroblasts / Q000737:chemistry; D006801:Humans; D007403:Intervertebral Disc / Q000737:chemistry*; D007719:Knee Joint; D008023:Ligaments, Articular / Q000737:chemistry*; D008592:Menisci, Tibial / Q000737:chemistry*; D010980:Platelet Membrane Glycoproteins / Q000032:analysis*; D012756:Sheep; D019699:Thrombospondins
D010980:Platelet Membrane Glycoproteins; D019699:Thrombospondins