title: Cerebrocortical oxygen supply of sclerotic rats and acute diltiazem therapy.
abstract: The therapeutic effects of calcium antagonist diltiazem on cerebrovascular smooth muscle of sclerotic and normal rats has been investigated. Arteriosclerosis was caused by treatment of the rats with a water soluble 1,25-dihydro-cholecalciferol in a dose of 97,000 IU. After 2-4 days, serum urea of the treated animals was increased from 56 +/- 8 to 78 +/- 17 mg/dl. Histological examinations of tissue probes from kidneys, heart, aorta and the major arteries by means of the staining method according to von Kossa clearly showed the picture of the Mönckeberg-type atherosclerosis. The calcified rats (N = 23) and the control group (N = 25) were anesthetized with ketamine/xylazine. Following craniotomy and tracheotomy for artificial ventilation, surface PO2 of the cerebro-cortex and cerebral blood flow via the inhalatory hydrogen clearance technique were measured. Surface PO2 frequency distribution of vitamin D3 treated animals showed a left shift (mean +/- SD = 29 +/- 8 compared with 34 +/- 6 mm Hg for controls) and a CBF decrease (1.36 +/- 0.51 compared to 1.50 +/- 0.60 ml/g.min for the controls). Diltiazem infusion of 2 ml/h corresponding to 5 micrograms/kg bw after 5 minutes caused a slight CBF decrease (1.50 +/- 0.60 to 1.43 +/- 0.78 ml/g.min) in controls and an insignificant CBF reduction in sclerotic rats. Mean arterial blood pressure was lowered by diltiazem infusion. The hypertensive sclerotic rats became almost normal (148 +/- 18 to 91 +/- 15 mm Hg) while the control animals decreased in MAP from 118 to 80 +/- 15 mm Hg.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Biosignalprocessing and Cybernetics, Medizinische Hochschule Hannover, FRG.
authors.forename: H P
authors.initials: HP
authors.lastname: Metzger
authors.forename: H
authors.initials: H
authors.lastname: Pante
authors.forename: S
authors.initials: S
authors.lastname: Heuber-Metzger
mesh_terms: D000818:Animals; D001161:Arteriosclerosis / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D002540:Cerebral Cortex / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism*; D002560:Cerebrovascular Circulation / Q000187:drug effects; D002762:Cholecalciferol; D003401:Creatine / Q000378:metabolism; D004110:Diltiazem / Q000627:therapeutic use; D005260:Female; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014508:Urea / Q000378:metabolism
chemical_list: D002762:Cholecalciferol; D014508:Urea; D004110:Diltiazem; D003401:Creatine; D010100:Oxygen