Effects of somatostatin-28 and some of its fragments and analogs on open-field behavior, barrel rotation, and shuttle box learning in rats.
The effect of intracerebroventricular (ICV) administration of somatostatin-28 (SS-28) and some of its fragments, SS-28 (1-12), SS-28(15-28), and analogs, des-AA-(1,2,4,5,12,13)-(DTrp8)SS-14, ODT8-SS, and (DTrp8,DCys14)SS-14 were studied in different behavioral tests on rats. SS-28 (6.0 nM) decreased, SS-28(15-28) (0.6 nM) increased, and SS-28(15-28) (6.0 nM) decreased activity of the animals in an open-field test. SS-28(1-12) (0.6, 3.0, and 6.0 nM) did not influence this behavior. ODT8-SS (0.6, 3.0, and 6.0 nM) decreased activity of the rats, while (DTrp8,DCys14)SS-14 increased it, only at a high dose (6.0 nM). Similar results were obtained for barrel rotation, except that (DTrp8,DCys14)SS-14 even in high dose (12.0 nM) had no effect. In a shuttle-box learning paradigm, SS-28(15-28) (0.6 nM) facilitated, while SS-28(1-12) (0.6 nM) did not influence, the performance of the animals. These results suggest that the isomeric Cys(28) form of the SS-28(15-28) molecule may be crucial for the expression of the behavioral effects of these peptides.
Vécsei
L
L
Department of Psychiatry and Neurochemistry, University of Lund, Sweden.
Widerlöv
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E
D000595:Amino Acid Sequence; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000494:pharmacology*; D011498:Protein Precursors / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000494:pharmacology*; D053561:Somatostatin-28
D010446:Peptide Fragments; D011498:Protein Precursors; D013004:Somatostatin; D053561:Somatostatin-28