Partition coefficients of fluorescent probes with phospholipid membranes.
A method for determination of membrane partition coefficients of five fluorescent membrane probes, 1,6-diphenyl-1,3,5-hexatriene (DPH), p-((6-phenyl)-1,3,5-hexatrienyl) benzoic acid (DPH carboxylic acid), 3-(p-(6-phenyl)-1,3,5-hexatrienyl)phenylpropionic acid (DPH propionic acid), 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) and N-4-(4-didecylaminostyryl)-N-methylpyridinium iodide (4-di-10-ASP), was developed utilizing the fluorescence enhancement of a constant probe concentration by titration with excess phospholipid liposomes. The partition coefficients of DPH, DPH carboxylic acid, DPH propionic acid, TMA-DPH and 4-di-10-ASP into dipalmitoylphosphatidylcholine membranes were determined to be 1.3.10(6), 1.0.10(6), 6.5.10(5), 2.4.10(5) and 2.8.10(6) respectively. Knowledge of the partition coefficients may help select a lipid concentration for membrane studies that necessitate a probe's dominant incorporation into membranes.
Huang
Z J
ZJ
Molecular Probes, Inc., Eugene, OR 97402.
Haugland
R P
RP
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry*; D007962:Leukocytes / Q000166:cytology; D008081:Liposomes; D051379:Mice; D013050:Spectrometry, Fluorescence / Q000379:methods; D013329:Structure-Activity Relationship
D005456:Fluorescent Dyes; D008081:Liposomes; D015060:1,2-Dipalmitoylphosphatidylcholine