title: A Drosophila Adh gene can be activated in trans by an enhancer.
abstract: The ability of a segment of the Drosophila Adh gene to produce ADH activity in larvae is dependent upon the presence of a 53 bp sequence (called NS1) located between 289 and 341 bp upstream of the larval transcription start site. This sequence behaves like an enhancer in that it can stimulate gene activity when it is placed at various distances from, or on either side of, an Adh gene. Like a typical enhancer, NS1 does not ordinarily function in trans. However, when an Adh gene lacking NS1 is placed on one plasmid, and a second gene carrying NS1 is placed on another, and the two plasmids are interlocked in a catenane, both genes are active. This finding supports the mechanism of loop-mediated enhancer action.
authors.affiliation: Department of Chemistry, Rutgers, State University of New Jersey, Newark 07102.
authors.forename: I
authors.initials: I
authors.lastname: Rothberg
authors.forename: E
authors.initials: E
authors.lastname: Hotaling
authors.forename: W
authors.initials: W
authors.lastname: Sofer
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics; D004742:Enhancer Elements, Genetic; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D010957:Plasmids; D015533:Transcriptional Activation
chemical_list: D004247:DNA; D000426:Alcohol Dehydrogenase