The amino acid-induced alteration in renal hemodynamics is glucagon independent.
The amino acid-induced alteration in renal hemodynamics is glucagon independent. An oral protein load or i.v. administration of an amino acid solution results in an increase in glomerular filtration rate and renal plasma flow in both humans and animals. The change in renal hemodynamics has been attributed to the simultaneous induced rise in glucagon. Whether glucagon is necessary for the change in renal hemodynamics after an amino acid infusion was investigated. Two groups of dogs were used, and the experimental protocol was divided into four different periods (P1 through P4). Group I animals received an amino acid solution, and group II dogs received an equiosmolar solution of mannitol. In P1, the animals in both groups were hydrated with normal saline, whereas, in P2, the pancreatic clamp technique was used to fix the plasma glucagon levels. P2 served as a basal period in which measurements of glomerular filtration rate, renal plasma flow, and plasma glucagon were obtained. IN P3, group I animals received amino acid solution, and group II received mannitol and served as controls. In this period, an increase of 32 and 27% in glomerular filtration rate and renal plasma flow, respectively, in group I dogs was observed, whereas there were no significant changes in these parameters in group II. During this period, plasma glucagon remained still at basal level in both groups. In P4, an infusion of glucagon at a rate of 5 ng/kg/min was added to both groups. This maneuver resulted in a fourfold increase in plasma glucagon levels in both groups.(ABSTRACT TRUNCATED AT 250 WORDS)
Nammour
T M
TM
Division of Nephrology, Vanderbilt University, Nashville, TN 37232.
Williams
P E
PE
Badr
K F
KF
Abumrad
N N
NN
Jacobson
H R
HR
D000596:Amino Acids / Q000097:blood / Q000494:pharmacology*; D000818:Animals; D004285:Dogs; D005215:Fasting; D005934:Glucagon / Q000097:blood / Q000502:physiology*; D015309:Glucose Clamp Technique; D006439:Hemodynamics / Q000187:drug effects; D006943:Hyperglycemia / Q000097:blood; D007328:Insulin / Q000097:blood; D012079:Renal Circulation / Q000187:drug effects*
D000596:Amino Acids; D007328:Insulin; D005934:Glucagon