title: Some electrospray mass spectrometric evidence for the existence of covalent O-acyl enzyme intermediates.
abstract: Electrospray mass spectrometry has been used to measure the masses of the species present in solutions of three serine proteases (alpha-chymotrypsin, subtilisin Carlsberg and subtilisin BPN') before, during and after completion of the hydrolytic reaction with cinnamoyl imidazole and indole acryloyl imidazole. The masses measured during the reaction demonstrated that covalent O-acyl enzyme intermediates had been formed.
authors.affiliation: Wellcome Research Laboratories, Beckenham, Kent, UK.
authors.forename: D S
authors.initials: DS
authors.lastname: Ashton
authors.forename: C R
authors.initials: CR
authors.lastname: Beddell
authors.forename: D J
authors.initials: DJ
authors.lastname: Cooper
authors.forename: B N
authors.initials: BN
authors.lastname: Green
authors.forename: R W
authors.initials: RW
authors.lastname: Oliver
authors.forename: K J
authors.initials: KJ
authors.lastname: Welham
mesh_terms: D002918:Chymotrypsin / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D008970:Molecular Weight; D013381:Subtilisins / Q000737:chemistry*
chemical_list: D013381:Subtilisins; D002918:Chymotrypsin