Differential detection of human papillomavirus DNA type 6 and 11 amplified by polymerase chain reaction.
We have developed a method for differential detection of human papillomavirus type 6 and 11 with selective amplification of a segment of the viral DNAs by the polymerase chain reaction. The target sequence for amplification was a 134-bp (bp 206-339) in the E6 open reading frame of HPV 6 and 11 DNAs. DNA extracted from a paraffin-section or a minute fresh biopsy specimen of condyloma acuminatum was amplified and hybridized with an oligonucleotide probe specific for HPV 6 or 11 DNA. The method was applied to analyse 5 cases of genital condyloma acuminatum, and as a result, HPV 6 and 11 DNAs were detected in two and one case, respectively. This method is useful for differential diagnosis of HPV 6 and 11 infection.
Liew
F M
FM
Department of Dermatology, Kyoto Prefectural University of Medicine, Japan.
Yamanishi
K
K
Kishimoto
S
S
Yasuno
H
H
D001483:Base Sequence; D003218:Condylomata Acuminata / Q000737:chemistry / Q000175:diagnosis / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D003937:Diagnosis, Differential; D005784:Gene Amplification / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D010412:Penile Neoplasms / Q000737:chemistry / Q000175:diagnosis / Q000235:genetics; D016133:Polymerase Chain Reaction; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000235:genetics / Q000382:microbiology
D004279:DNA, Viral; D015345:Oligonucleotide Probes