Continuous potable alcohol production by immobilized Saccharomyces cerevisiae on mineral kissiris.
A biocatalyst prepared by the immobilization of Saccharomyces cerevisiae on the surface of the mineral kissiris was used in the present study for continuous potable-alcohol production. An ethanol productivity (calculated on the basis of liquid volume) of 10.5 g/L/h was obtained at a 0.7/h dilution rate, 121 g/L sucrose content, and 29.6% conversion employing molasse as feed material. Glucose, raisin extracts, and molasse were successively used as feed materials without stopping the operation of the reactor for 6 mo. The ethanol productivity and yield remained constant during the operational-stability study of the reactor, carried out for 44 d. Biomass productivity, yield, and free-cell concentration in glucose, raisin extracts, and molasse were examined. Finally, a system with two continuous reactors joined successively was also studied in the present investigation.
Koutinas
A A
AA
Department of Chemistry, University of Patras, Greece.
Gourdoupis
C
C
Psarianos
C
C
Kaliafas
A
A
Kanellaki
M
M
D001709:Biotechnology; D003362:Cost-Benefit Analysis; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D008903:Minerals; D008965:Molasses; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D008903:Minerals; D000431:Ethanol; D005947:Glucose