cDNA-cloning and expression of VP1-specific sequences of foot-and-mouth disease virus types A5 and O1.
The RNA genome of foot- and mouth disease virus strains A5 Westerwald and O1 Lausanne has been reverse-transcribed and cloned in lambdaphages or plasmids. Identification of cDNA-clones containing VP1-specific sequences was achieved by hybridization, restriction mapping, and sequence analysis. VP1-coding cDNA-fragments were subcloned into the expression vector pEX which led to synthesis of fusion proteins with beta-galactosidase. These fusion proteins reacted with anti-VP1 antibodies on a Western blot, but were not capable of inducing neutralizing antibodies to mice. This seemed to suggest a tertiary structure of the VP1-epitopes unlike those of native VP1. Other attempts are discussed to construct VP1-fusion proteins folding similarly to the native viral protein structure.
Meyer
L
L
Friedrich-Loeffler-Institut Insel Riems.
Heinrich
H W
HW
Lenk
M
M
Kleinwächter
S
S
Horstmann
H
H
Bochnig
S
S
Eichler
P
P
Kruschke
P
P
D000595:Amino Acid Sequence; D000818:Animals; D005537:Aphthovirus / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry*; D015967:Gene Expression Regulation, Viral; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D014764:Viral Proteins