Growth and differentiation of Vero cells cultivated in three-dimensional type 1 collagen.
Vero cells grown in a three-dimensional arrangement of type 1 collagen were phenotypically altered. Such alterations consisted of variations in cellular form and reactivity to toluidine blue. After 22 days of incubation the Vero cells formed three-dimensional arrangements in both tubular form and cellular mass. A reduction in the collagenous substrate was observed in the culture and the alteration noted in the birefringence of the extracellular matrix was attributed to enzymatic action. This model demonstrates a good in vitro system for the study of collagenase activation and for in vitro tests with collagen.
Wada
M L
ML
Department of Cell Biology, UNICAMP, Campinas, Brazil.
Vidal
B de C
Bde C
D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology*; D003094:Collagen / Q000502:physiology*; D013710:Tendons / Q000502:physiology; D014709:Vero Cells
D003094:Collagen