title: Naloxone does not reverse the suppressive effects of testosterone infusion on luteinizing hormone secretion in pubertal boys.
abstract: In this study we wished to test whether, and if so when, the suppressive effects of testosterone on LH and, by inference, GnRH secretion are mediated via endogenous opioid pathways during male pubertal maturation. As a preliminary study, we evaluated the acute effects of a 24-h infusion of testosterone (T) in eight pubertal boys with constitutional delay of growth in order to determine the optimal time for administration of naloxone. Eight additional pubertal boys received a saline infusion, followed 1 week later by a similar T infusion starting at 1000 h and lasting for 33 h. After 2 h of infusion (both saline and T), four iv boluses of saline were given hourly, and after 26 h of infusion, four hourly iv boluses of naloxone were given. Blood was obtained every 15 min for LH and every 30 min for T measurements. T infusion increased the mean T concentration by 3.8-fold (P less than 0.001). Mean LH and LH pulse frequency were suppressed (P less than 0.01), and the sleep-associated increase in LH secretion was abolished. Naloxone administration during the infusion of T did not reverse the suppression of LH secretion. Compared to the saline control period, mean LH was significantly lower during T infusion during the time naloxone boluses were given (4.5 +/- 0.9 vs. 5.9 +/- 1.1 IU/L, T infusion and naloxone boluses vs. saline respectively, P less than 0.01). Although the suppression of LH pulse frequency remained significantly lower than that during the saline control period (0.23 +/- 0.04 pulses/boy.h during T infusion and saline boluses; 0.33 +/- 0.04 pulses/boy.h during T infusion plus naloxone boluses; 0.44 +/- 0.06 pulses/boy.h during saline infusion and saline boluses). Naloxone increased mean LH and LH pulse frequency only in the four older, more mature boys during the infusion of saline. Pituitary responsiveness to exogenous GnRH was not altered by infusion of T. We conclude that acute administration of T suppresses LH secretion and, by inference, GnRH secretion at all stages of pubertal maturation in boys. These negative feedback effects, however, cannot be reversed by coadministration of naloxone, even in mid- to late pubertal boys who respond to naloxone with increased pulsatile secretion of LH. These studies suggest that during pubertal maturation in boys, endogenous opioid pathways do not play a major role in the regulation of the negative feedback effects of T.
authors.affiliation: Department of Pediatrics, University of Michigan, Ann Arbor 48109-0718.
authors.forename: G B
authors.initials: GB
authors.lastname: Kletter
authors.forename: C M
authors.initials: CM
authors.lastname: Foster
authors.forename: M B
authors.initials: MB
authors.lastname: Brown
authors.forename: V
authors.initials: V
authors.lastname: Padmanabhan
authors.forename: I Z
authors.initials: IZ
authors.lastname: Beitins
authors.forename: J C
authors.initials: JC
authors.lastname: Marshall
authors.forename: R P
authors.initials: RP
authors.lastname: Kelch
mesh_terms: D000293:Adolescent; D004958:Estradiol / Q000097:blood; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D006801:Humans; D007262:Infusions, Intravenous; D007986:Luteinizing Hormone / Q000378:metabolism*; D008297:Male; D009270:Naloxone / Q000494:pharmacology*; D009994:Osmolar Concentration; D010902:Pituitary Gland / Q000187:drug effects; D011627:Puberty / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013739:Testosterone / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D007987:Gonadotropin-Releasing Hormone; D009270:Naloxone; D013739:Testosterone; D012965:Sodium Chloride; D004958:Estradiol; D007986:Luteinizing Hormone