title: Effect of dipyridamole on inosine triphosphate pyrophosphohydrolase activity and inosine triphosphate content in fresh human erythrocytes incubated with adenosine.
abstract: The activity of inosine triphosphate pyrophosphohydrolase (ITPH) in human erythrocytes was found to be 1.50 +/- 0.39 mumol of inosine triphosphate (ITP) hydrolysed x min-1 per g Hb, and no measurable amount of ITP was detected. When dipyridamole was added to the medium composed of adenosine, pyruvate and inorganic phosphate, ITPH activity was 1.18 +/- 0.41, and at the same time ITP accumulation was 0.61 +/- 0.31 mumol/g Hb. The negative correlation between ITPH activity and accumulation of ITP was r = -0.87 at P less than 0.001.
authors.affiliation: Department of Biochemistry, Medical Academy, Lódź, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Kopff
authors.forename: J
authors.initials: J
authors.lastname: Klem
authors.forename: I
authors.initials: I
authors.lastname: Zakrzewska
authors.forename: M
authors.initials: M
authors.lastname: Strzelczyk
mesh_terms: D000241:Adenosine / Q000378:metabolism*; D004176:Dipyridamole / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D007293:Inosine Triphosphate / Q000097:blood*; D011755:Pyrophosphatases / Q000097:blood*; D000097630:Inosine Triphosphatase
chemical_list: D000241:Adenosine; D004176:Dipyridamole; D007293:Inosine Triphosphate; D011755:Pyrophosphatases; D000097630:Inosine Triphosphatase