Alterations of pancreatic hepatocytes in rats exposed to carcinogens.
In this study, acute and chronic responses of pancreatic hepatocytes induced in F-344 rats by copper depletion-repletion protocol to certain hepatocarcinogens were examined. Administration of a single dose of tannic acid (subcutaneous), aflatoxin B1 (gavage), or lasiocarpine (intraperitoneally) caused characteristic nucleolar segregation in parenchymal cells of liver as well as in pancreatic hepatocytes. Chronic dietary administration of 2-acetylaminofluorene (0.025%) for 12 to 32 weeks led to the development of glutathione S-transferase-P-positive pancreatic hepatocytes in the pancreas. In addition, oval cell proliferation was observed in close association with pancreatic hepatocytes, but not in other areas of pancreas containing residual acinar cells. Oval cells in the pancreas and in the liver that developed in rats after chronic 2-acetylaminofluorene treatment and pancreatic duct cells stained positively with rat liver oval cell marker OV-6 antibodies by immunoperoxidase. These findings indicate that pancreatic hepatocytes respond to carcinogens in a fashion similar to parenchymal cells of liver.
Rao
M S
MS
Department of Pathology, Northwestern University Medical School, Chicago, Illinois 60611.
Subbarao
V
V
Sato
K
K
Reddy
J K
JK
D015073:2-Acetylaminofluorene / Q000008:administration & dosage / Q000494:pharmacology; D000284:Administration, Oral; D016604:Aflatoxin B1 / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002466:Cell Nucleolus / Q000187:drug effects; D005982:Glutathione Transferase / Q000032:analysis; D047348:Hydrolyzable Tannins / Q000008:administration & dosage / Q000494:pharmacology*; D007124:Immunoenzyme Techniques; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D008297:Male; D010179:Pancreas / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D010641:Phenotype; D011763:Pyrrolizidine Alkaloids / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344
D002273:Carcinogens; D047348:Hydrolyzable Tannins; D011763:Pyrrolizidine Alkaloids; D015073:2-Acetylaminofluorene; D016604:Aflatoxin B1; D005982:Glutathione Transferase; C005136:lasiocarpine