Multicompartmental secretion of ascorbate and its dual role in dopamine beta-hydroxylation.
The neurobiological functions of ascorbate have both intra- and extracellular sites of action. Intracellularly, it participates predominantly in enzymic and transport reactions for neurotransmitter and hormone biosynthesis. Ascorbate is the cofactor for the dopamine beta-hydroxylase and peptidylglycine alpha-amidating monooxygenase systems, which catalyze the synthesis of norepinephrine and a variety of alpha-amidated peptides, respectively. The localization of these enzymes within the neurotransmitter- or hormone-containing storage vesicle requires a system for the constant regeneration of ascorbate to the reduced form. In fact, ascorbate participates in its own regeneration as a component of the vesicular electron-transport system. In addition to the roles of ascorbate in messenger synthesis, it is secreted from cells from different subcellular compartments. The extracellular role(s) of ascorbate are still unknown, although its interaction with and modification of plasma membrane proteins suggests some modulatory function.
Diliberto
E J
EJ
Cellular and Molecular Pharmacology Section, Wellcome Research Laboratories, Research Triangle Park, NC 27709.
Daniels
A J
AJ
Viveros
O H
OH
D000313:Adrenal Medulla / Q000166:cytology / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism* / Q000502:physiology; D001692:Biological Transport; D002838:Chromaffin System / Q000166:cytology / Q000378:metabolism; D003683:Dehydroascorbic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism*; D006801:Humans; D007003:Hypoglycemia / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009097:Multienzyme Complexes; D010084:Oxidation-Reduction; D012769:Shock / Q000378:metabolism
D009097:Multienzyme Complexes; C000820:semidehydroascorbic acid; D006899:Mixed Function Oxygenases; D004299:Dopamine beta-Hydroxylase; C035189:peptidylglycine monooxygenase; D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid