Evaluation of monoclonal antibodies to Listeria monocytogenes flagella by checkerboard ELISA and cluster analysis.
A simple ELISA technique is described which utilizes whole bacteria as solid phase antigens for the evaluation of monoclonal antibodies against flagellar proteins of Listeria monocytogenes. Cluster analysis of the ELISA extinction values gave distinct reaction patterns from 23 tested supernatants. The method offers the possibility of screening supernatants without the necessity for standardization of antibody concentration or the labelling of established monoclonal antibodies.
Skjerve
E
E
Department of Food Hygiene, Norwegian College of Veterinary Medicine, Oslo.
Bos
W
W
van der Gaag
B
B
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D016000:Cluster Analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005407:Flagella / Q000276:immunology*; D008089:Listeria monocytogenes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal