title: [Tissue culture course of a human hepatoma cell line].
abstract: A cell line, HuH-33 was cultured in vitro from a patient with hepatocellular carcinoma. This cell line has been in continuous culture over 12 month period with slow growth potential. HuH-33 was composed spindle-or polygonal-shaped cells as a major population. Chromosome number of the cells were widely distributed even in the primary culture. HuH-33 was transplantable into nude mice and secreted alpha-fetoprotein, albumin, beta 2 microglobulin, ferritin and tissue polypeptide antigen.
authors.affiliation: Division of Pathology, Okayama University Medical School, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kusaka
authors.forename: T
authors.initials: T
authors.lastname: Tokiwa
authors.forename: N A
authors.initials: NA
authors.lastname: Monterio
authors.forename: A
authors.initials: A
authors.lastname: Muraoka
authors.forename: A
authors.initials: A
authors.lastname: Endo
authors.forename: J
authors.initials: J
authors.lastname: Sato
mesh_terms: D000368:Aged; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology*; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology*; D051379:Mice; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
chemical_list: D005819:Genetic Markers