title: Purification and functional characterization of the Glu-tRNA(Gln) amidotransferase from Chlamydomonas reinhardtii.
abstract: The formation of glutaminyl-tRNA (Gln-tRNA) in Bacilli, chloroplasts, and mitochondria occurs in a two-step reaction. This involves misacylation of tRNA(Gln) with glutamate by glutamyl-tRNA synthetase and subsequent amidation of Glu-tRNA(Gln) to the correctly acylated Gln-tRNA(Gln) by a specific amidotransferase (Schön, A., Kannangara, C. G., Gough, S., and Söll, D. (1988) Nature 331, 187-190). Here we demonstrate the existence of this pathway in green algae and describe the purification of the Glu-tRNA(Gln) amidotransferase from Chlamydomonas reinhardtii. The purified enzyme showed an Mr of approximately 120,000 when analyzed by glycerol gradient sedimentation and gel filtration. An apparent Mr of 63,000 of the denatured protein was demonstrated by electrophoresis on sodium dodecyl sulfate-polyacrylamide gels. This indicates that the enzyme possesses an alpha 2 structure. The substrate for the purified enzyme is Glu-tRNA(Gln) but not Glu-tRNA(Glu). The enzyme requires ATP, Mg2+, and an amide donor for the conversion. Acceptable amide donors are glutamine, asparagine, and ammonia. Blocking of the glutamine-dependent reaction by alkylation of the protein with 6-diazo-5-oxonorleucine did not inhibit the ammonia-dependent reaction, suggesting that the enzyme has separate glutamine and ammonia binding sites. As suggested by Wilcox (Wilcox, M. (1969) Eur. J. Biochem. 11, 405-412) the amidation reaction may involve glutamyl-phosphate formation, since ATP is cleaved to ADP when the enzyme is incubated with Glu-tRNA(Gln) and ATP. In common with other glutamine amidotransferases, the enzyme also possesses low glutaminase activity. The purified Glu-tRNA(Gln) amidotransferase forms a stable complex with Glu-tRNA(Gln) in the presence of ATP and Mg2+ but in the absence of the amide donor as determined by gradient centrifugation.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511.
authors.forename: D
authors.initials: D
authors.lastname: Jahn
authors.forename: Y C
authors.initials: YC
authors.lastname: Kim
authors.forename: Y
authors.initials: Y
authors.lastname: Ishino
authors.forename: M W
authors.initials: MW
authors.lastname: Chen
authors.forename: D
authors.initials: D
authors.lastname: Söll
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000641:Ammonia / Q000378:metabolism; D001216:Asparagine / Q000378:metabolism; D001391:Azo Compounds / Q000737:chemistry; D001665:Binding Sites; D002696:Chlamydomonas / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D005973:Glutamine / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D019884:Nitrogenous Group Transferases; D009646:Norleucine / Q000031:analogs & derivatives / Q000737:chemistry; D010766:Phosphorylation; D011489:Protein Denaturation; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D013053:Spectrophotometry; D013379:Substrate Specificity; D014166:Transferases / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D001391:Azo Compounds; D005971:Glutamates; D012346:RNA, Transfer, Amino Acyl; D005973:Glutamine; D018698:Glutamic Acid; D001216:Asparagine; D000641:Ammonia; D009646:Norleucine; D000255:Adenosine Triphosphate; D014166:Transferases; D019884:Nitrogenous Group Transferases; C047694:glutamyl-tRNA(Gln) amidotransferase; D008274:Magnesium; C000590535:6-diazo-5-oxonorleucine