Expression of bluetongue virus serotype 17 NS1 protein from a cloned gene.
A full-length copy of the coding region of segment 6 from bluetongue virus (BTV) serotype 17 was constructed from five overlapping cDNA clones. The gene coding for the NS1 protein was cloned into an expression plasmid under the control of a bacteriophage T7 promoter and expressed both in vitro and in Escherichia coli BL21(DE3) cells which contain a T7 RNA polymerase gene in their chromosome. Expression in both systems resulted in the synthesis of a protein comigrating with NS1 and a minor polypeptide comigrating with another viral-induced protein, NS1a, sometimes seen in BTV-infected cells. The proteins induced in E. coli were synthesized to high levels as insoluble products.
Grubman
M J
MJ
United States Department of Agriculture, ARS, NAA, Plum Island Animal Disease Center, Greenport, New York 11944.
D001820:Bluetongue virus / Q000235:genetics*; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005814:Genes, Viral; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013604:T-Phages / Q000235:genetics; D014758:Viral Core Proteins / Q000096:biosynthesis / Q000235:genetics*; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins
D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases