Activation of keratinization of keratinocytes from fetal rat skin with N-(O-linoleoyl) omega-hydroxy fatty acyl sphingosyl glucose (lipokeratinogenoside) as a marker of epidermis.
To elucidate the functional significance of sphingolipids altered in the epidermal differentiation, we examined, the effects of sphingolipids on the activity of transglutaminase and the formation of cornified envelopes in the keratinocytes from fetal rat skin. N-(O-linoleoyl) omega-hydroxy fatty acyl sphingosyl glucose (lipokeratinogenoside) that was characteristically contained in the mammalian epidermis, as well as nonhydroxy fatty acid-containing GalCer and GlcCer, significantly enhanced the activity and the formation, but no or rather inhibited activity was observed with ceramides, GalCer with alpha-hydroxy fatty acid, saponified lipokeratinogenoside, etc. This indicates that skin-characteristic lipokeratinogenoside functions to regulate the transglutaminase for the formation of cornified envelopes in the process of keratinization.
Uchida
Y
Y
Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.
Iwamori
M
M
Nagai
Y
Y
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002518:Ceramides / Q000494:pharmacology; D002554:Cerebrosides / Q000494:pharmacology; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005333:Fetus; D006028:Glycosphingolipids / Q000494:pharmacology*; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D012867:Skin / Q000254:growth & development*; D011503:Transglutaminases / Q000378:metabolism*
D002518:Ceramides; D002554:Cerebrosides; D015525:Fatty Acids, Omega-3; D006028:Glycosphingolipids; D011503:Transglutaminases