Muscle capillary-to-fiber perimeter ratio: morphometry.
It is known that a substantial amount of capillary tortuosity is found in shortened muscles. However, the increased capillary length and surface area contributed by tortuosity and branching are seldom taken into account when capillarity is estimated and/or blood-tissue exchange is modeled in muscles. In this paper, we sought morphometric estimates of capillarity in transverse sections that incorporated data on capillary geometry. We derived equations to estimate capillary perimeter per fiber perimeter (i.e., capillary-to-fiber perimeter ratio) in transverse sections. We show how capillary-to-fiber perimeter ratio is related to capillary surface per fiber surface, i.e., to the amount of capillary surface available for exchange per muscle fiber surface area, and how it can be obtained by morphometry. Because capillary tortuosity and fiber perimeter are both a function of sarcomere length, the degree of extension or shortening of muscle samples obviously needs to be taken into account when capillary-to-fiber perimeter ratio is compared between muscles and/or samples. Using data currently available on capillary length and diameter with fiber shortening and extension, we show that it is a feature of capillary-to-fiber perimeter ratio to change relatively little with sarcomere length. As sarcomere length decreases from 2.80 to 1.58 microns in perfusion-fixed hindlimb muscles of rats, capillary and fiber perimeters in transverse sections increase substantially, whereas the ratio between the two variables, capillary-to-fiber perimeter ratio, changes only less than or equal to 10-15%.(ABSTRACT TRUNCATED AT 250 WORDS)
Mathieu-Costello
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O
Department of Medicine, University of California, San Diego, La Jolla 92093-0623.
Ellis
C G
CG
Potter
R F
RF
MacDonald
I C
IC
Groom
A C
AC
D000818:Animals; D002196:Capillaries / Q000033:anatomy & histology / Q000502:physiology*; D002450:Cell Communication; D008433:Mathematics; D008955:Models, Cardiovascular; D009131:Muscle, Smooth, Vascular / Q000033:anatomy & histology / Q000502:physiology; D009132:Muscles / Q000033:anatomy & histology / Q000098:blood supply*; D012518:Sarcomeres / Q000502:physiology / Q000648:ultrastructure