Comparison of three serological techniques for the diagnosis of bovine herpesvirus type 1: serum neutralization, enzyme-linked immunosorbent assay and indirect immunofluorescence.
Serum neutralization (SN), indirect immunofluorescence (IFI) and enzyme-linked immunosorbent assay (ELISA) were compared for their sensitivity and specificity to detect bovine serum antibodies to Bovine Herpesvirus type 1 on 105 bovine serum samples. High correlation coefficients among all three techniques were observed, showing a higher sensitivity for the ELISA test. Due to its simplicity and its feasibility to be adopted in less equipped laboratories, the ELISA test is recommended for large scale epizotiological studies, serological diagnosis and detection of reactive animals.
Bratanich
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A
Instituto de Virología, INTA Castelar, Buenos Aires, Argentina.
Sardi
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S
Smitsaart
E
E
Estevez Madero
J
J
Schudel
A A
AA
D000818:Animals; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D007242:Herpesvirus 1, Bovine / Q000276:immunology / Q000302:isolation & purification*; D007241:Infectious Bovine Rhinotracheitis / Q000175:diagnosis*; D009500:Neutralization Tests; D012680:Sensitivity and Specificity