The influence of solvent stress on MMS-induced genetic change in Saccharomyces cerevisiae.
MMS induced mitotic recombination but not mitotic chromosome loss when tested in pure form in strain D61.M of Saccharomyces cerevisiae, confirming previous results of Albertini (1991), whereas in Aspergillus nidulans it also induced chromosomal malsegregation in addition to mitotic recombination (Käfer, 1988). However, induction of mitotic chromosome loss was observed in combination with strong inducers of chromosome loss such as the aprotic polar solvents ethyl acetate and to a lesser extent methyl ethyl ketone but not with gamma-valerolactone and propionitrile. In addition to this, 4 solvents, dimethyl formamide, dimethyl sulfoxide, dioxane and pyridine, enhanced the MMS-induced mitotic recombination in strain D61.M. An enhancement of MMS-induced mitotic recombination and reverse mutation could be demonstrated for ethyl acetate and gamma-valerolactone in yeast strain D7.
Zimmermann
F K
FK
Institut für Mikrobiologie, Technische Hochschule, Darmstadt, F.R.G.
Rohlfs
A
A
D000085:Acetates / Q000494:pharmacology; D002074:Butanones / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004347:Drug Interactions; D007783:Lactones / Q000494:pharmacology; D008741:Methyl Methanesulfonate / Q000633:toxicity*; D009152:Mutagenicity Tests; D009570:Nitriles / Q000494:pharmacology; D011995:Recombination, Genetic / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics; D012997:Solvents / Q000494:pharmacology*
D000085:Acetates; D002074:Butanones; D007783:Lactones; D009570:Nitriles; D012997:Solvents; C005222:methylethyl ketone; C007650:ethyl acetate; D008741:Methyl Methanesulfonate; C005557:propionitrile; D004121:Dimethyl Sulfoxide