Light and oxygen effects share a common regulatory DNA sequence in Rhodobacter capsulatus.
External factors regulate the formation of pigment protein complexes in facultatively photosynthetic bacteria. The puf operon of Rhodobacter capsulatus encodes the pigment binding proteins of the reaction centre and light-harvesting I complex. Here we demonstrate that a single base-pair exchange within a sequence of dyad symmetry upstream of the puf promoter affects both the oxygen regulation and the light regulation of the formation of reaction-centre and light-harvesting I complexes in Rhodobacter capsulatus. Our results imply that effects of oxygen or light ultimately act on the same regulatory DNA sequence, although it is still unknown how these environmental signals are sensed and transmitted to a transcriptional regulator.
Klug
G
G
Zentrum für Molekulare Biologie Heidelberg, Germany.
Gad'on
N
N
Jock
S
S
Narro
M L
ML
D000332:Aerobiosis; D001429:Bacteriochlorophylls / Q000096:biosynthesis; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000528:radiation effects; D005809:Genes, Regulator; D008027:Light; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D009876:Operon; D010100:Oxygen / Q000494:pharmacology*; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D016354:Rhodobacter capsulatus / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D015398:Signal Transduction; D014158:Transcription, Genetic
D001429:Bacteriochlorophylls; D004269:DNA, Bacterial; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D011993:Recombinant Fusion Proteins; D010100:Oxygen