Bilirubin and morphine glucuronidation is not inhibited by a cyclic-AMP mediated mechanism in murine liver.
Several reactions of the Ist and IInd phase of biotransformation have been reported to be under a negative cyclic-AMP dependent control. Conjugation of bilirubin and glucuronidation of morphine were investigated in isolated mouse hepatocytes and in mouse hepatocyte microsomes, respectively. N6,O2-dibutyryl cyclic-AMP did not inhibit conjugation of bilirubin in isolated hepatocytes. ATP and the dissociated catalytic subunit of the cyclic-AMP dependent protein kinase did not influence the glucuronidation of morphine, while inhibited the formation of p-nitrophenol glucuronide in microsomes prepared from isolated mouse hepatocytes.
Bánhegyi
G
G
1st Institute of Biochemistry, Semmelweis University, Medical School, Budapest, Hungary.
Garzó
T
T
Mészáros
G
G
Antoni
F
F
Mandl
J
J
D000818:Animals; D001663:Bilirubin / Q000031:analogs & derivatives* / Q000378:metabolism; D001711:Biotransformation / Q000187:drug effects; D003994:Bucladesine / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology*; D005965:Glucuronates / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D008862:Microsomes, Liver / Q000378:metabolism; D009020:Morphine / Q000378:metabolism*; D020113:Phenolphthalein; D010635:Phenolphthaleins / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism
D005965:Glucuronates; D010635:Phenolphthaleins; C002730:4-nitrophenylglucuronide; C016266:bilirubin glucuronate; D003994:Bucladesine; D020113:Phenolphthalein; D009020:Morphine; D000242:Cyclic AMP; D011494:Protein Kinases; D001663:Bilirubin