title: Modified low density lipoproteins activate human macrophages to secrete immunoreactive endothelin.
abstract: This study attempted to determine if low density lipoproteins (LDL) induce the production of endothelins (ET) by human macrophages. Non-protected LDL from macrophage induced oxidation (n-LDL), copper-oxidized LDL (Ox-LDL), acetylated-LDL (Ac-LDL), butylated hydroxytoluene-LDL (BHT-LDL), BHT-Ac-LDL, polyinosinic acid (PiA, 1.5 micrograms/ml), phorbol myristate acetate (PMA; 0.5 microM) and BHT alone (20 microM) were studied. The different compounds had the following potency to stimulate the ET secretion: PMA greater than Ox-LDL greater than Ac-LDL greater than n-LDL greater than BHT-LDL greater than PiA greater than PiA + Ac-LDL greater than BHT. In conclusion, modified LDL stimulated ET secretion by human macrophages.
authors.affiliation: Department of Lipoproteins and Atherosclerosis Research, Pasteur Institute, Lille, France.
authors.forename: F
authors.initials: F
authors.lastname: Martin-Nizard
authors.forename: H S
authors.initials: HS
authors.lastname: Houssaini
authors.forename: S
authors.initials: S
authors.lastname: Lestavel-Delattre
authors.forename: P
authors.initials: P
authors.lastname: Duriez
authors.forename: J C
authors.initials: JC
authors.lastname: Fruchart
mesh_terms: D001665:Binding Sites; D002478:Cells, Cultured; D016232:Endothelins / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D016232:Endothelins; D008077:Lipoproteins, LDL; D013755:Tetradecanoylphorbol Acetate