Determination of leukotriene B4 in human plasma by gas chromatography using a mass selective detector and a stable isotope labelled internal standard. Effect of NE-11740 on arachidonic acid metabolism.
A highly selective gas chromatographic method, coupled with selected ion monitoring using a mass selective detector and positive electron ionization, was developed for the determination of leukotriene B4 (LTB4) in human plasma. Plasma was separated from whole human blood via centrifugation, proteins precipitated with acetonitrile and LTB4 recovered (approximately 82.0%) by ethyl acetate extraction. The methyl ester, bis-t-butyldimethylsilyl ether derivative of LTB4 was formed prior to analysis and determined quantitatively using [18O]2-LTB4 as an internal standard. The limit of detection (S/N = 2) was 425 pg on column (m/z 335/339) using a 1-microliter injection. Standard curves were linear over two orders of magnitude with an RSD of less than 5.0% (n = 10). NE-11740, a new anti-inflammatory drug, was shown to inhibit, in a dose-dependent manner (ED50 = 22 microM) ionophore-stimulated LTB4 biosynthesis by human whole blood in vitro.
Doyle
M J
MJ
Procter & Gamble Company, Miami Valley Laboratories, H&PCTD, Cincinnati, OH 45239-8707.
Eichhold
T H
TH
Hynd
B A
BA
Weisman
S M
SM
D000480:Alkynes / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007975:Leukotriene B4 / Q000097:blood*; D010636:Phenols / Q000494:pharmacology*
D000480:Alkynes; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001095:Arachidonic Acids; D010636:Phenols; D007975:Leukotriene B4; D016718:Arachidonic Acid; C064268:tebufelone