Use of 33P for Sanger DNA sequencing.
The use of [alpha-33P]deoxyadenosine 5'-triphosphate ([alpha-33P]dATP) in DNA sequencing has been described. 33P has a maximum beta-emission energy that is 50% stronger than 35S, but fivefold weaker than 32P. As a result, sequences generated using [alpha-33P]dATP have short exposure times like 32P, yet they maintain band resolution similar to 35S. Handling of [alpha-33P]dATP is straightforward because no special lead or Plexiglas shielding is necessary.
Zagursky
R J
RJ
Du Pont Merck Pharmaceutical Company, Research and Development, Wilmington, DE 19880-0328.
Conway
P S
PS
Kashdan
M A
MA
D001483:Base Sequence; D004247:DNA; D003838:Deoxyadenine Nucleotides; D005821:Genetic Techniques; D010761:Phosphorus Radioisotopes
D003838:Deoxyadenine Nucleotides; D010761:Phosphorus Radioisotopes; D004247:DNA; C026600:2'-deoxyadenosine triphosphate