title: Structure of the K24 antigen of E. coli O83:K24:H, a polymer that consists of alpha-Kdop and glycerol phosphate.
abstract: The structure of the K24 antigen of Escherichia coli O83:K24:H31 was elucidated by determination of composition and by 1H-, 13C-, and 31P-n.m.r. spectroscopy of the polymer and of a Kdo-glycerol (Gro) glycoside, obtained by mild alkaline hydrolysis and subsequent incubation with alkaline phosphatase. The K24 antigen has the repeating unit----7)-alpha-Kdop-(2----1)-Gro-(3-P. In the polymer, 56% of the repeating units are O-acetylated at C-4 of Kdo, approximately 28% at C-5 of Kdo, and approximately 16% are not acetylated.
authors.affiliation: Max-Planck-Institut für Immunbiologie, Freiburg-Zähringen, F.R.G.
authors.forename: M C
authors.initials: MC
authors.lastname: Lenter
authors.forename: B
authors.initials: B
authors.lastname: Jann
authors.forename: K
authors.initials: K
authors.lastname: Jann
mesh_terms: D000107:Acetylation; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D001704:Biopolymers; D002236:Carbohydrate Conformation; D002247:Carbon Isotopes; D004926:Escherichia coli / Q000276:immunology*; D005994:Glycerophosphates / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010504:Periodic Acid; D013400:Sugar Acids / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000942:Antigens, Bacterial; D001704:Biopolymers; D002247:Carbon Isotopes; D005994:Glycerophosphates; C068278:K24 antigen; D013400:Sugar Acids; D010504:Periodic Acid; C002532:2-keto-3-deoxyoctonate; C009288:metaperiodate