Effects of verapamil on synaptic transmission in mammalian sympathetic ganglia.
Effects of verapamil (Ver) on the synaptic transmission of isolated superior cervical ganglia (SCG) were investigated by means of intracellular recording techniques. In the neurons of SCG of rabbits and guinea pigs, Ver (100-400 mumol/L) depressed both the synaptic responses (f-EPSPs and/or evoked action potentials) elicited by orthodromic stimulation and the acetylcholine (ACh) potentials induced by pressure ejection of ACh. 4-Aminopyridine (4-AP, 100 mumol/L) and BaCl2 (5 mmol/L) facilitated the f-EPSPs inhibited by Ver, but had no notable effect on the ACh potentials depressed by Ver. Ver suppressed the ACh potentials enhanced by pyridostigmine (1 mumol/L). The potentiating effect of soman (1 mumol/L) on the ACh potentials was prevented by Ver. It is suggested that Ver can block the synaptic transmission of mammalian SCG by both the pre- and postganglionic mechanisms.
Liu
H Q
HQ
Department of Pharmacology, Loyola University Medical Center, Maywood, IL 60153.
Dun
N J
NJ
D000200:Action Potentials / Q000187:drug effects; D000631:Aminopyridines / Q000494:pharmacology; D000818:Animals; D001464:Barium / Q000494:pharmacology; D017609:Barium Compounds; D002712:Chlorides; D005728:Ganglia, Sympathetic / Q000187:drug effects* / Q000502:physiology; D006168:Guinea Pigs; D008297:Male; D009474:Neurons / Q000502:physiology; D011729:Pyridostigmine Bromide / Q000037:antagonists & inhibitors; D011817:Rabbits; D012999:Soman / Q000037:antagonists & inhibitors; D013569:Synapses / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects*; D014700:Verapamil / Q000494:pharmacology*
D000631:Aminopyridines; D017609:Barium Compounds; D002712:Chlorides; C024986:barium chloride; D001464:Barium; D012999:Soman; D014700:Verapamil; D011729:Pyridostigmine Bromide