Ferromagnetic isolation of endosomes involved in vitellogenin transfer into Xenopus oocytes.
The transport pathway of the yolk precursor vitellogenin (VTG) has been followed using the techniques of ferrolabeling and ferromagnetic sorting, coupled with electron microscopic visualization. Vitellogenin conjugated to colloidal ferric particles of ca. 11 nm is selectively transported from the oolemma to the yolk platelets of vitellogenic Xenopus oocytes after gonadotropin stimulation of the female. Several cortical membrane compartments, labeled or unlabeled with ferric particles, are involved in the internalization and the transfer of vitellogenin to the yolk platelets. 1) Coated pits apparently fuse with coated vesicles, and coated vesicles fuse with each other in the outermost cortical cytoplasm. 2) Vesicles, depleted of their clathrin coat, fuse with cortical tubular endosomes and discharge their contents into yolk endosomes. 3) These endosomes are the direct precursors of the yolk organelles. 4) Endocytic vesicles fuse only with primordial yolk platelets of type I and not with type II or fully grown yolk platelets. After pulse-chase loading with ferric particles conjugated to vitellogenin and subsequent subcellular fractionation of the oocytes, ferromagnetic sorting of the various vesicle populations has been performed by using a "free-flow magnetic chamber". This novel method enables specification and characterization of purified endosomal compartments that accumulate protein yolk in Xenopus oocytes.
Richter
H P
HP
Department of Physiology, University of the Saarland, Homburg/Saar, Federal Republic of Germany.
Bauer
A
A
D000818:Animals; D001692:Biological Transport; D003034:Coated Pits, Cell-Membrane / Q000648:ultrastructure; D003102:Colloids; D004705:Endocytosis; D011992:Endosomes / Q000648:ultrastructure; D005260:Female; D006651:Histocytochemistry; D007501:Iron; D008280:Magnetics; D008854:Microscopy, Electron; D009865:Oocytes / Q000378:metabolism* / Q000648:ultrastructure; D015388:Organelles / Q000378:metabolism* / Q000648:ultrastructure; D014819:Vitellogenins / Q000378:metabolism*; D014982:Xenopus laevis
D003102:Colloids; D014819:Vitellogenins; D007501:Iron