Binding of colloidal gold-labeled salivary proline-rich proteins to Actinomyces viscosus type 1 fimbriae.
Salivary proline-rich proteins (PRPs), which were purified from parotid saliva, were adsorbed onto 15-nm-diameter gold particles to visualize specific binding of the salivary molecules to Actinomyces viscosus type 1 fimbriae. Negatively stained preparations incubated with PRP-gold conjugates but not bovine serum albumin-gold complexes bound specifically to bacteria possessing type 1 fimbriae, A. viscosus T14V-J1 and 5519. Binding of the PRP-gold probes to strains deficient in type 1 fimbriae, i.e., strains 5951 (type 2 fimbriae only) and 147 (no fimbriae), was negligible.
Leung
K P
KP
Department of Oral Biology, College of Dentistry, University of Florida, Gainesville 32610.
Nesbitt
W E
WE
Fischlschweiger
W
W
Hay
D I
DI
Clark
W B
WB
D000190:Actinomyces / Q000378:metabolism* / Q000648:ultrastructure; D001422:Bacterial Adhesion; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D006046:Gold; D006801:Humans; D066298:In Vitro Techniques; D007526:Isoelectric Point; D008854:Microscopy, Electron; D010455:Peptides / Q000378:metabolism*; D055232:Proline-Rich Protein Domains; D011485:Protein Binding; D012471:Salivary Proteins and Peptides / Q000378:metabolism*
D010455:Peptides; D012471:Salivary Proteins and Peptides; D006046:Gold