title: Potentiation of human natural killer cell activity by recombinant interleukin-2 towards multidrug-resistant human epidermoid carcinoma.
abstract: Relatively little is understood about the antigen recognition and target structures used by natural killer (NK) cells. The purpose of this study was to analyze the relationship of a multidrug-resistant cell line expressing the P-glycoprotein (GP170 surface glycoprotein) derived from the parent non-drug-resistant malignant tumor cell line for susceptibility to lysis by either NK or lymphokine-activated killer cells. Our results demonstrate no significant difference in NK activity against either the non-drug-resistant or drug-resistant malignant cell line; Interleukin-2 induces a significant increase in cytolytic activity toward the multidrug-resistant cell line. These data suggest that malignant cells refractory to treatment or occurring after successful treatment are susceptible to immunotherapeutic intervention.
authors.affiliation: Biological Products Branch, Centers for Disease Control, US Department of Health and Human Services, Atlanta, Ga.
authors.forename: E W
authors.initials: EW
authors.lastname: Ades
authors.forename: D
authors.initials: D
authors.lastname: Bosse
authors.forename: J
authors.initials: J
authors.lastname: Pruckler
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000628:therapy; D003602:Cytotoxicity, Immunologic; D004351:Drug Resistance; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D007167:Immunotherapy; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000008:administration & dosage*; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000502:physiology*; D009303:Nasopharyngeal Neoplasms / Q000276:immunology* / Q000628:therapy; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015395:Histocompatibility Antigens Class I; D007376:Interleukin-2; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins