title: Capsaicin-sensitive nerves are required for glucostasis but not for catecholamine output during hypoglycemia in rats.
abstract: We have studied the glucose response and catecholamine (CA) response to insulin in the conscious rat to evaluate the role of sensory fibers in these responses in animals pretreated with capsaicin as neonates. In contrast to previous results obtained in anesthetized rats (Z. Khalil, B.G. Livett, and P.D. Marley. J. Physiol. Lond. 370: 201-215, 1986; Z. Khalil, B.G. Livett, and P.D. Marley. J. Physiol. Lond. 391: 511-526, 1987.), in conscious rats, insulin (1 IU/kg iv) produced only a mild hypoglycemia, which quickly returned to resting levels and caused no significant changes in plasma epinephrine levels. Somatostatin and SMS-(201-995), a somatostatin analogue, both potentiated and prolonged the insulin-induced hypoglycemia, resulting in an increase in circulating CA levels that was suppressed by hexamethonium and atropine. In capsaicin-pretreated rats the blood glucose levels at 90 min after insulin were significantly lower than those in vehicle-pretreated rats both in the presence (1 IU/kg insulin, 48 +/- 6 vs. 92 +/- 6 mg/100 ml, P less than 0.01) and absence (10 IU/kg insulin, 38 +/- 4 vs. 51 +/- 2 mg/100 ml, P less than 0.01) of SMS-(201-995). The CA levels in capsaicin-pretreated rats at 90 min after insulin were higher than in vehicle-pretreated rats (epinephrine levels: 27 +/- 4 vs. 10 +/- 1 pmol/ml in 1 IU/kg insulin, P less than 0.01; 64 +/- 14 vs. 25 +/- 5 pmol/ml in 10 IU/kg insulin, P less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: X F
authors.initials: XF
authors.lastname: Zhou
authors.forename: K H
authors.initials: KH
authors.lastname: Jhamandas
authors.forename: B G
authors.initials: BG
authors.lastname: Livett
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001786:Blood Glucose / Q000378:metabolism*; D001835:Body Weight / Q000187:drug effects; D002211:Capsaicin / Q000494:pharmacology*; D004837:Epinephrine / Q000097:blood*; D018738:Hexamethonium; D006584:Hexamethonium Compounds / Q000494:pharmacology; D007003:Hypoglycemia / Q000097:blood* / Q000139:chemically induced; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D009638:Norepinephrine / Q000097:blood*; D015282:Octreotide / Q000494:pharmacology; D051381:Rats; D011915:Rats, Inbred BUF; D012016:Reference Values; D013004:Somatostatin / Q000494:pharmacology
chemical_list: D001786:Blood Glucose; D006584:Hexamethonium Compounds; D007328:Insulin; D018738:Hexamethonium; D013004:Somatostatin; D015282:Octreotide; D002211:Capsaicin; D009638:Norepinephrine; D004837:Epinephrine