Production of human to mouse xenografts by umbilical cord blood.
Utilizing human umbilical cord blood, it has been possible to create in irradiated animals a human to mouse xenograft. To facilitate hematopoietic reconstitution, SJL/J mice, which are functionally low in natural killer (NK) cells, were treated with anti-Asialo GM1 antibodies (anti-NK) and irradiation prior to injection of cord blood mononuclear cells. In contrast, SJL/J mice with the "beige" (bg/bg) mutation, which confers a functional NK cell deficiency, required only irradiation for successful transplantation. Human cells, detected by means of DNA probes, were demonstrated in the lungs and lymph nodes of irradiated animals up to 6 months after injection of the human cord blood cells.
Ende
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N
Department of Pathology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
Giuliani
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D
Ende
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M
Ponzio
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NM
D000818:Animals; D001803:Blood Transfusion; D015139:Blotting, Southern; D015342:DNA Probes; D005260:Female; D005312:Fetal Blood / Q000276:immunology; D005677:G(M1) Ganglioside / Q000008:administration & dosage; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006801:Humans; D007116:Immunization, Passive; D007694:Killer Cells, Natural / Q000276:immunology / Q000528:radiation effects; D008168:Lung / Q000166:cytology; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008817:Mice, Mutant Strains; D011247:Pregnancy; D014183:Transplantation, Heterologous; D014916:Whole-Body Irradiation
D015342:DNA Probes; D005677:G(M1) Ganglioside