Transfer region of IncI1 plasmid R64 and role of shufflon in R64 transfer.
To locate the transfer region of the 122-kiloase plasmid R64drd-11 belonging to incompatibility group I1, a series of deletion derivatives was constructed by in vitro recombinant DNA techniques followed by double homologous recombination in vivo. A plasmid designated pKK609 and bearing a 56.7-kilobase R64 sequence was the smallest transferable plasmid. A plasmid designated pKK610 and no longer possessing the 44-base-pair sequence of the R64 transfer system is located at one end. The other end of the R64 transfer region comprises a DNA segment of about 19 kilobases responsible for pilus formation. Shufflon, DNA with a novel rearrangement in R64, was found to be involved in pilus formation.
Komano
T
T
Department of Biology, Tokyo Metropolitan University, Japan.
Funayama
N
N
Kim
S R
SR
Nisioka
T
T
D002872:Chromosome Deletion; D003001:Cloning, Molecular; D003090:Coliphages; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D010861:Fimbriae, Bacterial; D009154:Mutation; D011815:R Factors / Q000235:genetics*; D015183:Restriction Mapping
D004251:DNA Transposable Elements