title: Three-dimensional structure of holo 3 alpha,20 beta-hydroxysteroid dehydrogenase: a member of a short-chain dehydrogenase family.
abstract: The x-ray structure of a short-chain dehydrogenase, the bacterial holo 3 alpha,20 beta-hydroxysteroid dehydrogenase (EC 1.1.1.53), is described at 2.6 A resolution. This enzyme is active as a tetramer and crystallizes with four identical subunits in the asymmetric unit. It has the alpha/beta fold characteristic of the dinucleotide binding region. The fold of the rest of the subunit, the quaternary structure, and the nature of the cofactor-enzyme interactions are, however, significantly different from those observed in the long-chain dehydrogenases. The architecture of the postulated active site is consistent with the observed stereospecificity of the enzyme and the fact that the tetramer is the active form. There is only one cofactor and one substrate-binding site per subunit; the specificity for both 3 alpha- and 20 beta-ends of the steroid results from the binding of the steroid in two orientations near the same cofactor at the same catalytic site.
authors.affiliation: Medical Foundation of Buffalo, NY 14203.
authors.forename: D
authors.initials: D
authors.lastname: Ghosh
authors.forename: C M
authors.initials: CM
authors.lastname: Weeks
authors.forename: P
authors.initials: P
authors.lastname: Grochulski
authors.forename: W L
authors.initials: WL
authors.lastname: Duax
authors.forename: M
authors.initials: M
authors.lastname: Erman
authors.forename: R L
authors.initials: RL
authors.lastname: Rimsay
authors.forename: J C
authors.initials: JC
authors.lastname: Orr
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology; D003196:Computer Graphics; D003349:Cortisone Reductase / Q000737:chemistry*; D046911:Macromolecular Substances; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity; D014961:X-Ray Diffraction
chemical_list: D046911:Macromolecular Substances; D010088:Oxidoreductases; D003349:Cortisone Reductase