Differential mRNA levels of c-myc, c-fos and MHC class I in several clones of a murine fibrosarcoma.
We have evaluated the relationship between MHC class-I, c-myc and c-fos proto-oncogene expression in several clones of a methylcholanthrene-induced fibrosarcoma (GR9) which originated in a BALB/c mouse. These clones represent a heterogeneous population and differ markedly with regard to H-2 class-I cell-surface expression, local tumor growth, NK sensitivity and metastatic potential. In the present study we show that cell-surface expression of MHC class-I antigens correlates inversely with levels of c-myc mRNA transcripts. On the other hand, mRNA levels of c-fos are correlated directly with the amount of mRNA of MHC class I. Treatment of the B9 clone with gamma interferon increased mRNA transcription and surface expression of H-2 class-I antigens, while c-myc transcription was simultaneously down-regulated. In contrast, c-fos mRNA levels remained unaltered.
Gaforio
J J
JJ
Servicio de Análisis Clínicos e Inmunologia, Hospital Virgen de las Nieves, Universidad de Granada, Spain.
Pérez
M
M
Algarra
I
I
Mialdea
M J
MJ
Ljunggren
H G
HG
Garrido
F
F
D000818:Animals; D015152:Blotting, Northern; D002999:Clone Cells; D005354:Fibrosarcoma / Q000235:genetics* / Q000276:immunology; D005434:Flow Cytometry; D005805:Genes, MHC Class I; D016762:Genes, fos; D016259:Genes, myc; D006183:H-2 Antigens / Q000032:analysis / Q000235:genetics; D007371:Interferon-gamma / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D012513:Sarcoma, Experimental / Q000235:genetics* / Q000276:immunology; D014158:Transcription, Genetic / Q000187:drug effects
D006183:H-2 Antigens; D012333:RNA, Messenger; D007371:Interferon-gamma