Determination of octopine by pre-column fluorescence derivatization using benzoin.
A sensitive fluorimetric method for the determination of octopine, a member of opine family, is presented. The method is based on the formation of a fluorescent derivative of octopine with benzoin and the separation by high performance liquid chromatography using a reversed-phase column (Kaseisorb LC ODS-300) within 20 min. The octopine derivative is completely separated from other guanidino compounds including arginine which is generally very high in marine invertebrates. This method gives higher sensitivity, 5 pmol minimum detection, and better reproducibility than the electrophoresis method and colorimetric method.
Sato
M
M
Faculty of Agriculture, Tohoku University, Sendai, Japan.
Takeuchi
M
M
Kanno
N
N
Nagahisa
E
E
Sato
Y
Y
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis; D001573:Benzoin; D002851:Chromatography, High Pressure Liquid; D005470:Fluorometry / Q000379:methods*; D008974:Mollusca / Q000032:analysis
C005394:octopine; D001120:Arginine; D001573:Benzoin