African swine fever virus fatty acid acylated proteins.
Labeling experiments with [3H]palmitic and [3H]myristic acids of African swine fever virus-infected Vero cells have shown that 11 proteins induced during infection are covalently bound to myristic acid and that palmitic acid was not attached to viral proteins. The time course of synthesis of the myristylated polypeptides and the requirements of viral DNA replication indicated that the myristylated proteins, with the exception of a 13-kDa protein, belong to the late class of viral proteins. The myristic moiety was not released by hydroxylamine treatment, suggesting that the fatty acid is bound to the polypeptide chain through an amide linkage. The purification of [3H]myristic acid-labeled extracellular virus particles demonstrated that the myristylated 28- and 13-kDa proteins incorporated into the virion.
Aguado
B
B
Centro de Biología Molecular (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma, Madrid, Spain.
Viñuela
E
E
Alcamí
A
A
D000215:Acylation; D000358:African Swine Fever Virus / Q000378:metabolism*; D000818:Animals; D004261:DNA Replication; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000378:metabolism*; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000494:pharmacology; D007700:Kinetics; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*; D014709:Vero Cells; D014764:Viral Proteins / Q000187:drug effects / Q000378:metabolism*
D005227:Fatty Acids; D006898:Hydroxylamines; D009227:Myristic Acids; D010169:Palmitic Acids; D014764:Viral Proteins; D019814:Myristic Acid; D019811:Hydroxylamine; D019308:Palmitic Acid