title: Haemolytic and phospholipase A activities of the tentacle extract of Catostylus mosaicus.
abstract: An extract prepared from the tentacle of Catostylus mosaicus was shown to lyse erythrocytes from rat, rabbit and human to a different extent; those from the rat being most susceptible followed by those from rabbit and human. The haemolytic activity was dependent on the concentration of crude extract protein exhibiting a sigmoidal curve. Only 60% of the haemolytic activity was retained after treament with heat and proteolytic enzyme. The extract was devoid of hydrolytic enzymes normally present in venoms except for phospholipase A activity, which resulted in the hydrolysis of membrane phospholipids with concomittant appearance of their lyso-derivatives.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysis.
authors.forename: N
authors.initials: N
authors.lastname: Azila
authors.forename: I
authors.initials: I
authors.lastname: Othman
mesh_terms: D000818:Animals; D003064:Cnidarian Venoms / Q000633:toxicity*; D004910:Erythrocyte Membrane / Q000378:metabolism; D006461:Hemolysis; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D008563:Membrane Lipids / Q000032:analysis / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D013997:Time Factors; D014357:Trypsin / Q000378:metabolism
chemical_list: D003064:Cnidarian Venoms; D008563:Membrane Lipids; D010740:Phospholipases; D010741:Phospholipases A; D010447:Peptide Hydrolases; D014357:Trypsin