Immunoglobulins and monoclonal anti-blastocyst antibodies in the mouse uterine secretion during the early pre-implantation period.
A Sepharose bead blot technique was used to study immunoglobulins in the uterine secretion of mice during the pre-implantation stage. Secretion collected by Sepharose beads contained IgA, IgG, and IgM. The method could be made ten-fold more sensitive by using anti-mouse IgG or IgM conjugated to Sepharose beads. It has also been demonstrated that when injected intravenously, biotinylated purified immunoglobulins, both non-specific mouse myeloma IgG and IgM and specific anti-blastocyst IgG and IgM, is able to pass into the uterine cavity of the mouse. It was further shown that when injected systemically, anti-blastocyst antibodies can reach the blastocyst. Functionally active specific antibodies against morulae and/or blastocysts may, therefore, be able to influence the pre- and peri-implantation development of the embryo and could serve as a useful model for experiments directed towards the identification of immunological contraceptive procedures.
Hjortberg
M
M
Department of Human Anatomy, Uppsala University, Sweden.
Jin
M
M
Larsson
A
A
Nilsson
B O
BO
D000818:Animals; D000911:Antibodies, Monoclonal; D001755:Blastocyst / Q000187:drug effects / Q000276:immunology*; D047108:Embryonic Development / Q000276:immunology*; D005260:Female; D015151:Immunoblotting / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D007150:Immunohistochemistry; D007275:Injections, Intravenous; D051379:Mice; D010066:Ovum Transport / Q000187:drug effects / Q000502:physiology; D011247:Pregnancy; D012685:Sepharose; D014599:Uterus / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D012685:Sepharose