Rapid evaluation of topoisomerase inhibitors: caffeine inhibition of topoisomerases in vivo.
Caffeine was found to inhibit both type I and type II topoisomerases in vivo as judged by its effects on replicating simian virus 40 (SV40) chromosomes. The study was facilitated by the use of a rapid filter assay for the detection and characterization of topoisomerase inhibitors. The assay, which requires neither purified enzymes nor substrates, was able to identify both antagonists and poisons of type I and type II topoisomerases.
Shin
C G
CG
Department of Radiology, Ohio State University, Columbus 43210.
Strayer
J M
JM
Wani
M A
MA
Snapka
R M
RM
D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D002166:Camptothecin / Q000494:pharmacology; D005047:Etoposide / Q000494:pharmacology; D000882:Haplorhini; D013713:Teniposide / Q000494:pharmacology; D059004:Topoisomerase I Inhibitors; D059005:Topoisomerase II Inhibitors
D059004:Topoisomerase I Inhibitors; D059005:Topoisomerase II Inhibitors; D002110:Caffeine; D005047:Etoposide; D013713:Teniposide; D002166:Camptothecin