[Interaction of DNA with divalent metal ions].
The interaction between the native DNA macromolecules and Ca2+, Mn2+, Cu2+ ions in solutions of low ionic strength (10(-3) M Na+) is studied using the methods of differential UV spectroscopy and CD spectroscopy. It is shown that the transition metal ions Mn2+ exercise binding to the nitrogen bases of DNA at concentrations approximately 5 x 10(-6) M and form chelates with guanine of N7-Me(2+)-O6 type. Only at high concentrations in solution (5 x 10(-3) M) do Ca2+ ions interact with the nitrogen bases of native DNA. In the process of binding to Ca2+ and Mn2+ the DNA conformation experiences some changes under which the secondary structure of the biopolymer is within the B-form family. The DNA transition to the new conformation is revealed by its binding to Cu2+ ions.
Kornilova
S V
SV
Sorokin
V A
VA
Blagoĭ
Iu P
IuP
Valeev
V A
VA
Arutiunian
S G
SG
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002413:Cations, Divalent; D002417:Cattle; D002942:Circular Dichroism; D003300:Copper / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D008345:Manganese / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D013056:Spectrophotometry, Ultraviolet
D002413:Cations, Divalent; D008345:Manganese; D003300:Copper; D004247:DNA; D002118:Calcium
Izuchenie vzaimodeĭstviia DNK s ionami dvukhvalentnykh metallov.