title: Collagen, proteoglycan and hyaluronidase activity in cultures from normal and scoliotic chicken fibroblasts.
abstract: Connective tissue matrix components were investigated using skin fibroblasts from normal or inbred scoliotic lines of chickens. Specifically, the fibroblasts were obtained from either an isogenic line or a backcross, derived by crossing the isogenic line with a pure line of scoliotic birds. From the backcross, both affected (35-45%) and non-affected (55-65%) progeny were produced. The affected birds had spinal curves greater than 20 degrees. Several abnormalities of connective tissue were observed when cells from scoliotic chicks were grown in culture: increased collagen extractability, decreased aggregatability of proteoglycans under associative conditions and lower than normal levels of hyaluronic acid. There was also less collagen deposited in the cell layer with proportionately increased amounts of collagen secreted into the culture media by cells from scoliotic versus normal chick fibroblasts. Values for collagen matrix stability, as estimated by extractability and net deposition, were intermediate for cells from the backcrossed, but non-affected, birds. Moreover, hyaluronidase, an enzyme that degrades hyaluronic acid, was abnormally elevated in the fibroblast cultures from scoliotic chicks. It is proposed that the increase in hyaluronidase contributes to the abnormalities observed in extracellular matrix components and may be a factor in the expression of scoliosis in susceptible birds.
authors.affiliation: Department of Pathology, University of California, San Francisco.
authors.forename: Y H
authors.initials: YH
authors.lastname: Lien
authors.forename: J
authors.initials: J
authors.lastname: Fu
authors.forename: R B
authors.initials: RB
authors.lastname: Rucker
authors.forename: M
authors.initials: M
authors.lastname: Scheck
authors.forename: U
authors.initials: U
authors.lastname: Abbott
authors.forename: R
authors.initials: R
authors.lastname: Stern
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D003094:Collagen / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000378:metabolism; D006821:Hyaluronoglucosaminidase / Q000378:metabolism*; D046911:Macromolecular Substances; D008249:Protein-Lysine 6-Oxidase / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism*; D012600:Scoliosis / Q000378:metabolism*
chemical_list: D046911:Macromolecular Substances; D011509:Proteoglycans; D006820:Hyaluronic Acid; D003094:Collagen; D008249:Protein-Lysine 6-Oxidase; D006821:Hyaluronoglucosaminidase