No evidence of HTLV-I infection in French patients with multiple sclerosis using the polymerase chain reaction.
The polymerase chain reaction (PCR), using three primer pairs in the pol, tax, and env regions of the HTLV-I genome, was unable to detect HTLV-I in the blood samples of 54 caucasian subjects with multiple sclerosis who were seronegative for HTLV-I/II. Seventeen HTLV-I/II seropositive (by ELISA and Western blot) subjects used as positive controls were positive with the three primer pairs. The PCR was negative in 47 healthy HTLV-I/II seronegative (by ELISA) subjects at low risk of HTLV-I infection used as negative controls. These results suggest that there is no association between the occurrence of HTLV-I sequences and the development of multiple sclerosis.
Wattel
E
E
Institut National de Transfusion Sanguine, Paris, France.
Mariotti
M
M
Bignon
J D
JD
Agis
F
F
Gordien
E
E
Muller
J Y
JY
Hors
J
J
Lefrère
J J
JJ
D000328:Adult; D005260:Female; D015490:HTLV-I Infections / Q000175:diagnosis*; D015368:Human T-lymphotropic virus 1 / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D009103:Multiple Sclerosis / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods