title: Detection of Streptococcus suis type 2 in tonsils of slaughtered pigs using improved selective and differential media.
abstract: New selective and differential media were devised for the isolation and identification of Streptococcus suis type 2. The selective medium (NNCC) agar) was Todd-Hewitt broth containing 1.5% Bactoagar and 5% defibrinated sheep blood with addition of sodium azide (50 micrograms/ml), nalidixic acid (25 micrograms/ml), colistin (12.5 micrograms/ml) and crystal violet (2 micrograms/ml). The differential medium consisted of heart infusion agar and antiserum specific only for S. suis type 2. In a total of 291 pigs tested by a combination of these media, S. suis type 2 was isolated and confirmed from 40 of them (13.7%).
authors.affiliation: First Research Division, National Institute of Animal Health, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kataoka
authors.forename: C
authors.initials: C
authors.lastname: Sugimoto
authors.forename: M
authors.initials: M
authors.lastname: Nakazawa
authors.forename: M
authors.initials: M
authors.lastname: Kashiwazaki
mesh_terms: D000362:Agar; D000818:Animals; D001386:Azides; D003091:Colistin; D003470:Culture Media; D005840:Gentian Violet; D007564:Japan; D009268:Nalidixic Acid; D014066:Palatine Tonsil / Q000382:microbiology*; D019810:Sodium Azide; D013291:Streptococcus / Q000302:isolation & purification*; D013552:Swine / Q000382:microbiology*
chemical_list: D001386:Azides; D003470:Culture Media; D009268:Nalidixic Acid; D000362:Agar; D019810:Sodium Azide; D005840:Gentian Violet; D003091:Colistin