title: Expression of bovine leukaemia virus envelope gene by recombinant vaccinia viruses.
abstract: Recombinant vaccinia viruses (VV) containing the envelope gene of bovine leukaemia virus (BLV) were constructed. Three virus constructs were designed: VV-BLV1 which contained the open reading frame for envelope glycoprotein gp51 alone, under control of VVP7.5 promoter; VV-BLV2 and VV-BLV3 contained the entire gene (gp51 + gp30) coding sequence downstream of VP7.5 and the fowlpox virus early/late promoter (PFE/L) respectively. All three VV recombinants expressed envelope glycoproteins as determined by the agar gel diffusion assay. By immunofluorescence techniques it was shown that while VV-BLV2 and VV-BLV3 expressed envelope glycoprotein on the surface of virus-infected cells, VV-BLV1 failed to do so. Rabbits inoculated with VV-BLV1 failed to show an anti envelope glycoprotein antibody response, however, significant levels of antibodies against envelope glycoprotein were detected in sera from rabbits inoculated with VV-BLV2 and VV-BLV3.
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory, Geelong, Victoria.
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: M E
authors.initials: ME
authors.lastname: Andrew
authors.forename: D B
authors.initials: DB
authors.lastname: Boyle
authors.forename: R B
authors.initials: RB
authors.lastname: Brandon
authors.forename: M F
authors.initials: MF
authors.lastname: Lavin
authors.forename: R C
authors.initials: RC
authors.lastname: Daniel
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral; D015752:Genes, env; D005779:Immunodiffusion; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D010957:Plasmids; D011233:Precipitin Tests; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000276:immunology; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014616:Vaccinia virus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000276:immunology
chemical_list: D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins