[Transformation of various strains of Escherichia coli by multicopy plasmids with the phoA gene leads to secretion of a periplasmatic alkaline phosphatase into the media and accumulation of its precursor].
Various E. coli strains have been transformed by multicopy plasmids pHI-1, pHI-7 and pPHO I carrying the entire regulatory and structural phoA sequences. All the transformants with the intact pho regulatory system have been shown to be capable of alkaline phosphatase oversynthesis and secretion into the medium. They are also able to accumulate the alkaline phosphatase precursor localized in the outer membrane fraction. The transformants of the restriction or recombination mutants are the most efficient producers of the extracellular enzyme and its precursor.
Nesmeianova
M A
MA
Karamyshev
A L
AL
Tsfasman
I M
IM
Fedotikova
E T
ET
D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000378:metabolism; D015153:Blotting, Western; D002462:Cell Membrane / Q000201:enzymology; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D010957:Plasmids; D014170:Transformation, Genetic
D003470:Culture Media; D004269:DNA, Bacterial; D004792:Enzyme Precursors; D000469:Alkaline Phosphatase
Transformatsiia razlichnykh shtammov Escherichia coli mul'tikopiĭnymi plazmidami s genom phoA pribodit k sekretsii periplazmaticheskoĭ shchelochnoĭ fosfatazy v sredu i nakopleniiu ee predshestvennika.