cDNA cloning and regulation of a novel rat cytochrome P450 of the 2C gene subfamily (P450IIC24).
A novel member of the cytochrome P450 2C gene subfamily was identified by screening rat prostate cDNA libraries. Two independent clones were isolated. Clone pros1 was 1031 bp long and contained a bizarre replacement in place of putative exon 1. Clone pros2 was 1755 bp long, contained a complete 3' end, and also had bizarre sequences in place of exon 1, which in this case were compatible with an unspliced intron. Northern analysis revealed mRNA expression in the liver and the kidney. Interestingly, although livers of mature rats of both sexes have comparable amounts of P4502C24 mRNA, a dramatic sex difference is seen in the kidney where only males express detectable levels of this mRNA.
Zaphiropoulos
P G
PG
Department of Medical Nutrition, Huddinge University Hospital, Sweden.
D000375:Aging; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular / Q000379:methods; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005091:Exons; D005260:Female; D015723:Gene Library; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology / Q000254:growth & development; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family; D011467:Prostate / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D012689:Sequence Homology, Nucleic Acid
D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System