Expression of terminal deoxynucleotidyltransferase gene in a case of adult T-cell leukemia.
A 37-year-old male from Kagoshima Prefecture was admitted with adult T-cell leukemia (ATL). Monoclonal integration of HTLV-1 proviral DNA was found, but the integration site was different from that of terminal deoxynucleotidyltransferase (TdT)-positive MT-1 cells (an ATL cell line). The ATL cells expressed enzymatically active TdT and exhibited 2100 b TdT mRNA, which corresponds to the thymus type of TdT mRNA. The same size of TdT mRNA was also detected in MT-1. Southern blot analyses revealed no differences in the gene structure of the promoter region of TdT genes between this ATL case and TdT-positive lymphoblastic leukemia cells. There is little possibility that cis-acting viral elements promote TdT gene expression by proviral integration. The activation of TdT gene in ATL may be mediated by other trans-acting factors.
Oiwa
K
K
Department of Pediatrics, Nagoya University School of Medicine, Japan.
Horibe
K
K
Koiwai
O
O
Kaneda
T
T
Nakaide
Y
Y
Tamori
S
S
Kodaka
T
T
Uchiyama
T
T
D000328:Adult; D004253:DNA Nucleotidylexotransferase / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D015458:Leukemia, T-Cell / Q000235:genetics*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics
D012333:RNA, Messenger; D004253:DNA Nucleotidylexotransferase