Simultaneous determination of morazone and phenmetrazine in rat plasma and urine using an on-column injection technique with fused-silica capillary column gas chromatography.
The simultaneous determination of morazone and its major metabolite, phenmetrazine, using an on-column injection technique with fused-silica capillary column gas chromatography is described. The on-column injection technique prevents the decomposition of morazone. The limit of quantitation in 0.1 mL of rat plasma for morazone and phenmetrazine is 5 ng/mL and that in 1.0 mL of rat urine is 1 ng/mL. The within run precisions (%CV) at the concentration of 0.5 micrograms/mL are 3.27% for morazone and 3.09% for phenmetrazine.
Lho
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Doping Control Center, Korea Institute of Science and Technology, Kangnam-ku, Seoul.
Shin
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Park
J
J
D000701:Analgesics, Opioid / Q000097:blood* / Q000652:urine; D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D002849:Chromatography, Gas / Q000379:methods; D010633:Phenmetrazine / Q000097:blood* / Q000652:urine; D051381:Rats; D015203:Reproducibility of Results
D000701:Analgesics, Opioid; C009385:morazone; D000983:Antipyrine; D010633:Phenmetrazine