title: Synthesis of 6 beta-hydroxyaldosterone by A6 (toad kidney) cells in culture.
abstract: Incubation of aldosterone with confluent layers of A6 (toad kidney) cells leads to its hydroxylation at the 6 beta-position. 6 beta-Hydroxyaldosterone is the major metabolite when the incubation is carried out at pH 6.8, whereas the product comprises 6 beta-hydroxy-17-isoaldosterone accompanied by some 6 beta-hydroxyapoaldosterone at pH 7.4. All products were identified by high-field 1H nuclear magnetic resonance spectroscopy. Control experiments indicated that the side-chain isomerization to form the 17-iso and apo derivatives occurs after the cytochrome P 450-dependent synthesis of 6 beta-hydroxyaldosterone.
authors.affiliation: Department of Laboratory Medicine, Miriam Hospital, Providence, RI 02906.
authors.forename: D J
authors.initials: DJ
authors.lastname: Morris
authors.forename: S A
authors.initials: SA
authors.lastname: Latif
authors.forename: T J
authors.initials: TJ
authors.lastname: Conca
authors.forename: L T
authors.initials: LT
authors.lastname: Wei
authors.forename: C O
authors.initials: CO
authors.lastname: Watlington
authors.forename: D N
authors.initials: DN
authors.lastname: Kirk
authors.forename: H C
authors.initials: HC
authors.lastname: Toms
authors.forename: C H
authors.initials: CH
authors.lastname: Shackleton
mesh_terms: D000450:Aldosterone / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System; D008401:Gas Chromatography-Mass Spectrometry; D007668:Kidney / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011522:Protons; D013250:Steroid Hydroxylases / Q000378:metabolism*; D013379:Substrate Specificity; D014981:Xenopus
chemical_list: D011522:Protons; C067782:6-hydroxyaldosterone; D000450:Aldosterone; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; C022657:steroid hormone 6-beta-hydroxylase