Beta-D-glucuronidase activity assay for rapid differentiation of species within beta-haemolytic group C and G streptococci.
Methylumbelliferyl-conjugated enzyme substrates were assessed for their ability to differentiate beta haemolytic streptococci in Lancefield groups C and G. Both Streptococcus equisimilis (group C) and large colony human biotype group G strains were consistently differentiated from group C and G "Streptococcus milleri group" bacteria by their ability to hydrolyse the beta-D-glucuronide substrate. The test was completed in less than one hour.
Cimolai
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Department of Pathology, University of British Columbia, Canada.
Mah
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D
D001431:Bacteriological Techniques; D002648:Child; D005966:Glucuronidase / Q000378:metabolism*; D006801:Humans; D010614:Pharynx / Q000382:microbiology*; D013291:Streptococcus / Q000145:classification* / Q000201:enzymology; D013997:Time Factors
D005966:Glucuronidase