Determination of phenolalkylamines, narcotic analgesics, and beta-blockers by gas chromatography/mass spectrometry.
An analytical procedure for determination of phenolalkylamines, narcotic analgesics, and beta-blockers in urine by gas chromatography/mass spectrometry (GC/MS) is described. The detection of phenolalkylamines, narcotic analgesics, and beta-blockers is based on acid hydrolysis, liquid-liquid extraction, and selective derivatization. For screening of phenolalkylamines the m/e 179 and 267 ions were monitored by GC/MS. With narcotic analgesics, the extracted ion corresponded to the molecular ion (M+) of the drug and two additional characteristic ions. Beta-blockers were analyzed as the selectively derivatized forms of the parent molecule and its metabolites by GC/MS with selected ion monitoring. The ions monitored for screening of beta-blockers containing an isopropylamine group were m/e 284 and 129 ions. The ion at m/e 86 was monitored to characterize the tert-butylamine group of beta-blockers.
Lho
D S
DS
Doping Control Center, Korea Institute of Science and Technology, Cheongryang, Seoul.
Hong
J K
JK
Paek
H K
HK
Lee
J A
JA
Park
J
J
D000319:Adrenergic beta-Antagonists / Q000652:urine*; D000701:Analgesics, Opioid / Q000652:urine*; D004300:Doping in Sports; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D015813:Substance Abuse Detection / Q000379:methods*; D013566:Sympathomimetics / Q000652:urine*
D000319:Adrenergic beta-Antagonists; D000701:Analgesics, Opioid; D013566:Sympathomimetics