CD8+ T cells inhibit HIV replication in naturally infected CD4+ T cells. Evidence for a soluble inhibitor.
This study describes the inhibitory effect exerted by activated CD8+ T cells on the replication of HIV in naturally infected CD4+ T cells. Highly purified CD4+ T cells from asymptomatic HIV seropositive individuals were stimulated with anti-TCR mAb-coated beads in the presence of IL-2. HIV was subsequently reproducibly isolated in cell supernatants from all study participants (53 cultures from 42 individuals). Both autologous and allogeneic CD8+ T cells from asymptomatic HIV seropositive and healthy HIV seronegative individuals inhibited the replication of HIV in these cultures in a dose-dependent manner. CD8+ T cells from patients with AIDS showed reduced or no such inhibitory activity. The inhibitory effect was not dependent on direct cell-cell contact: an inhibitory effect was exerted by CD8+ T cells across a semipermeable membrane, and an inhibitory activity was also exerted by the cell-free supernatants from activated CD8+ T cells. These results suggest that activated CD8+ T cells secrete a soluble inhibitor of HIV replication.
Brinchmann
J E
JE
Institute of Transplantation Immunology, National Hospital, Oslo, Norway.
Gaudernack
G
G
Vartdal
F
F
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D000943:Antigens, Differentiation / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000382:microbiology*; D016827:CD8 Antigens; D002478:Cells, Cultured; D015683:Gene Products, gag / Q000032:analysis; D016655:HIV Core Protein p24; D015497:HIV-1 / Q000254:growth & development*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1; D008222:Lymphokines / Q000502:physiology*; D016028:Receptors, Leukocyte-Adhesion / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology*; D014758:Viral Core Proteins / Q000032:analysis; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D015683:Gene Products, gag; D016655:HIV Core Protein p24; D015395:Histocompatibility Antigens Class I; D016169:Lymphocyte Function-Associated Antigen-1; D008222:Lymphokines; D016028:Receptors, Leukocyte-Adhesion; D014758:Viral Core Proteins