title: Preliminary report: accurate assays for anti-HIV in urine.
abstract: Untreated urine specimens from 358 patients (344 attending genito-urinary medicine clinics, 14 haemophiliacs) and 353 blood donors were tested blind by a simple IgG-capture particle-adherence test (GACPAT) and a rapid IgG-capture enzyme-linked immunosorbent assay (GACELISA) for antibody to human immunodeficiency virus (anti-HIV). All 158 urine specimens from seropositive subjects were anti-HIV positive by GACPAT and 157 of them (99.4%) were positive by GACELISA. Tests on 553 urine specimens from seronegative subjects gave two repeatable false-positive reactions by GACPAT (0.4%) and none by GACELISA. By means of a modified procedure anti-gp160 was detected by commercial western blot in the urine of 44 of 45 seropositive subjects examined. IgG-capture assays will detect anti-HIV in unconcentrated urine and so allow a diagnosis in circumstances when blood sampling is impracticable.
authors.affiliation: PHLS Virus Reference Laboratory, London, UK.
authors.forename: J A
authors.initials: JA
authors.lastname: Connell
authors.forename: J V
authors.initials: JV
authors.lastname: Parry
authors.forename: P P
authors.initials: PP
authors.lastname: Mortimer
authors.forename: R J
authors.initials: RJ
authors.lastname: Duncan
authors.forename: K A
authors.initials: KA
authors.lastname: McLean
authors.forename: A M
authors.initials: AM
authors.lastname: Johnson
authors.forename: M H
authors.initials: MH
authors.lastname: Hambling
authors.forename: J
authors.initials: J
authors.lastname: Barbara
authors.forename: C P
authors.initials: CP
authors.lastname: Farrington
mesh_terms: D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005189:False Positive Reactions; D015483:HIV Antibodies / Q000652:urine*; D006801:Humans; D007104:Immune Adherence Reaction / Q000379:methods; D007074:Immunoglobulin G / Q000652:urine; D007158:Immunologic Techniques; D012680:Sensitivity and Specificity
chemical_list: D015483:HIV Antibodies; D007074:Immunoglobulin G