title: Ultrastructural in vitro characterization of a porous hydroxyapatite/bone cell interface.
abstract: In order for the hydroxyapatite implant material interface to new bone to be characterized, osteogenic mouse calvarial mesenchymal cells were grown in vitro in contact with a porous hydroxyapatite (PHA). After the mesenchymal cell culture was incubated for 12 to 13 days, the resulting tissue-containing bone colonies were fixed, embedded, sectioned, and stained for microscopic evaluation. Light and transmission electron microscopy (with conventional staining) and phosphotungstic acid cytochemistry were used to explore and record the optical microscopic and ultrastructural interfaces at the hydroxyapatite surface. Osteoblasts, fibroblasts, bone, and cartilage were observed and photographed at the implant surface. Osteoblasts found in conjunction with well-developed collagen, matrix vesicles in the extracellular matrix, and newly formed hydroxyapatite crystals on the PHA surface confirmed the beginning of woven bone formation. Collagen fibers were observed directly in contact with the PHA when osteoblasts were present. Polysaccharides were localized among the collagen fibers in the implant-cell extracellular space, indicating a rich complex carbohydrate layer in relation to the collagen of immature bone. Fibroblasts and chondroblasts at the implant surface secreted no collagen, but an amorphous layer was visible between the fibroblasts and the implant surface. When polysaccharides were stained, an electron-dense film appeared where the amorphous layer came into contact with the implant material. Collagen was secreted from the cell surface furthest from the implant. Osteoblasts and fibroblasts/chondroblasts, when surrounding PHA, seem to take on two different interfacial functions: Osteoblasts secrete collagen in a bone-initiating extracellular matrix with carbohydrates at the implant surface, whereas fibroblasts/chondroblasts appear to attach to the implant with a carbohydrate-rich attachment substance, but with no collagen at the interface. This study confirms in vivo data that PHA is a viable implant material because it is biocompatible and, unlike several other materials, appears to stimulate, or at least to permit, osteogenesis.
authors.affiliation: Department of Oral Biology, University of California, Los Angeles 90024.
authors.forename: C M
authors.initials: CM
authors.lastname: Holden
authors.forename: G W
authors.initials: GW
authors.lastname: Bernard
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001842:Bone and Bones / Q000166:cytology*; D002356:Cartilage / Q000166:cytology; D015921:Dental Implants; D017886:Durapatite; D005347:Fibroblasts; D006882:Hydroxyapatites; D051379:Mice; D016348:Osseointegration; D010006:Osteoblasts
chemical_list: D001672:Biocompatible Materials; D015921:Dental Implants; D006882:Hydroxyapatites; D017886:Durapatite