Leucocyte function in rats with naturally occurring gingivitis.
The severity of gingivitis in relation to abnormal macrophage chemotaxis and superoxide (O2-) production was investigated in rats with naturally occurring gingivitis (plaque-susceptible rats). Macrophage chemotaxis was measured by the membrane filter method; zymosan-activated serum was used as a chemo-attractant. O2- production was measured by the reduction of cytochrome c method. Opsonized zymosan, phorbol myristate acetate and calcium ionophore A23187 were used as stimulants. The macrophage chemotaxis and O2- production in the plaque-susceptible rats with deep pockets were significantly lower than those in the susceptible rats with shallow pockets or the normal group. In addition, there was a negative correlation between the severity of gingivitis in plaque-susceptible rats and macrophage chemotaxis or O2- production. These findings suggest that impairement of the host defence mechanisms due to the reduction in macrophage chemotaxis and O2- production is related to the progression of periodontal disease in plaque-susceptible rats.
Ohura
K
K
Department of Pharmacology, Osaka Dental University, Japan.
Shinohara
M
M
Ogata
K
K
Nishiyama
A
A
Mori
M
M
D000818:Animals; D002464:Cell Migration Inhibition; D002633:Chemotaxis / Q000502:physiology*; D003773:Dental Plaque; D004198:Disease Susceptibility; D005891:Gingivitis / Q000473:pathology*; D008264:Macrophages / Q000378:metabolism / Q000502:physiology*; D010514:Periodontal Pocket / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D013481:Superoxides / Q000378:metabolism*
D013481:Superoxides