title: Characteristics of human arterial smooth muscle cell cultures infected with cytomegalovirus.
abstract: Distinct, sequential events occurring during the destruction and simultaneous regrowth of human arterial smooth muscle cell (SMC) cultures infected with cytomegalovirus (CMV, AD169 strain) were characterized. The events were influenced by the typical phenotypic diversity reflecting relative states of differentiation of the SMC cultures. Progenitors of regeneration were a surviving population of small, undifferentiated or relatively undifferentiated SMCs. As these cells reached confluence focally, the number of cells reactive with antismooth muscle serum, i.e. differentiating, increased, and in some postconfluent foci the organization of SMCs resembled the topography of uninfected cultures. Thus, infected SMC cultures had a limited capacity to repopulate, to organize typically, and to differentiate. However, continuing cytopathic effects gradually destroyed much of the regrowth, and a relatively large, nondividing SMC with prominent cytoplasmic filaments, similar to SMCs in terminal, uninfected cultures, predominated. Infected cultures consisting overwhelmingly of the large terminal phenotype were far less productive of infectious CMV than cultures populated by SMCs with continuing capacity to divide. Gradually, cultures consisting of the terminal phenotype deteriorated as a result of sporadic cytopathic effects of CMV and an effect resembling "senescent" degeneration in uninfected, nondividing cultures in late passage. The infected, terminal phenotype could be a latent or persistent source of CMV antigen or nucleic acid-positive cells detected by different investigators in normal and in atheromatous, human tissue, assuming that it exists and survives for an extended period in vivo after infection of vascular SMC.
authors.affiliation: Department of Surgery, Baylor College of Medicine, Houston, Texas 77030.
authors.forename: J J
authors.initials: JJ
authors.lastname: Tumilowicz
mesh_terms: D000906:Antibodies / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000302:isolation & purification / Q000502:physiology; D003586:Cytomegalovirus Infections / Q000382:microbiology / Q000473:pathology*; D003588:Cytopathogenic Effect, Viral; D005455:Fluorescent Antibody Technique; D006801:Humans; D009130:Muscle, Smooth / Q000276:immunology; D009131:Muscle, Smooth, Vascular / Q000382:microbiology / Q000473:pathology*; D010641:Phenotype; D014469:Umbilical Arteries / Q000473:pathology
chemical_list: D000906:Antibodies; D000914:Antibodies, Viral