A rapid method for the quantitation of estrogen receptors in small amounts of tissue.
A rapid and highly reproducible protocol which permits the detection of quantities of estrogen receptor as low as 5 fmol/mg protein is described. The separation of free and receptor-bound hormone is achieved by specific immunoprecipitation of the hormone-receptor complex. This procedure can be performed without perturbing the equilibrium of the binding reaction.
Bettini
E
E
Milano Molecular Pharmacology Lab, University of Milan, Italy.
Maggi
A
A
D000818:Animals; D003600:Cytosol / Q000737:chemistry; D005260:Female; D051379:Mice; D051381:Rats; D011919:Rats, Inbred Strains; D011960:Receptors, Estrogen / Q000032:analysis* / Q000276:immunology; D015203:Reproducibility of Results
D011960:Receptors, Estrogen