Trophoblast cell differentiation: establishment, characterization, and modulation of a rat trophoblast cell line expressing members of the placental prolactin family.
The purpose of this investigation was to establish and characterize a cell line derived from a rat choriocarcinoma and to evaluate the usefulness of the cell line as an in vitro model for studying trophoblast cell differentiation. A cell line was generated from choriocarcinoma explants and named Rcho-1. The cell line consisted of a mixture of cell types, including small cells growing in clusters and giant cells possessing very large nuclei. This characteristic morphology was maintained through at least 23 passages and in a series of clonal cell lines isolated from the parent Rcho-1 cell line. The Rcho-1 cell line was capable of expressing placental lactogen-I (PL-I), PL-II, PRL-like protein-A (PLP-A), and PLP-C mRNAs when cultivated in vitro; however, the Rcho-1 cells expressed only PL-I when grown beneath the kidney capsule of host rats. The Rcho-1 cell line did not express PLP-B under any experimental condition. This pattern of placental PRL expression was maintained for 23 passages. Rcho-1 cells synthesized and secreted PL-I, PL-II, and PLP-A proteins with biochemical characteristics similar to those of their placental counterparts. PL-I and PL-II mRNAs were specifically localized to giant cells. Morphological appearance and placental PRL expression were used as indices for monitoring the differentiation state of Rcho-1 cells grown under various conditions. Both morphological and functional trophoblast cell differentiation were induced by maintaining the Rcho-1 cells in postconfluent culture conditions. Postconfluent Rcho-1 cultures were characterized by an increased percentage of giant cells and an induction of placental PRL expression. Some clonal cell lines derived from the parent Rcho-1 cell line exhibited distinct patterns of differentiation and placental PRL expression. In summary, we have established a rat trophoblast cell line capable of expressing a differentiated phenotype. The differentiated phenotype includes both morphological and functional parameters and can be modulated in vitro. This cell line is a unique model for studying the control of placental PRL gene expression and the regulation of trophoblast cell differentiation.
Faria
T N
TN
Department of Physiology, University of Kansas Medical Center, Kansas City 66103.
Soares
M J
MJ
D000818:Animals; D015152:Blotting, Northern; D002454:Cell Differentiation; D002455:Cell Division; D002822:Choriocarcinoma; D005260:Female; D015870:Gene Expression; D007163:Immunosorbent Techniques; D008297:Male; D010920:Placenta / Q000378:metabolism*; D010928:Placental Lactogen / Q000235:genetics; D011257:Pregnancy Proteins / Q000235:genetics; D011388:Prolactin / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism; D014407:Tumor Cells, Cultured
D011257:Pregnancy Proteins; C502143:Prl4a1 protein, rat; C502145:Prl8a3 protein, rat; D012333:RNA, Messenger; C062303:placental lactogen II; D011388:Prolactin; D010928:Placental Lactogen