A monoclonal antibody to DNA modified with osmium tetroxide/2,2'-bipyridine.
A murine monoclonal antibody (IgG) has been generated that binds to DNA modified with osmium tetroxide in the presence of 2,2'-bipyridine and does not interact with unmodified DNA. Reactivity of the antibody was tested by gel retardation assay, ELISA, dot-binding assay and immunoblotting. The results obtained suggest that the antibody does not cross-react with modified or unmodified RNA or proteins. The high specificity of the binding reaction is due to the specific recognition of modified deoxythymidine residue by the monoclonal antibody. A possible way of using the antibody produced is discussed.
Kabakov
A E
AE
Institute of Medical Radiology, Academy of Medical Sciences, Obninsk, Kaluga Region, U.S.S.R.
Podgorodnichenko
V K
VK
Poverenny
A M
AM
D015082:2,2'-Dipyridyl / Q000737:chemistry*; D000818:Animals; D000974:Antibodies, Antinuclear / Q000096:biosynthesis / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D004247:DNA / Q000737:chemistry / Q000276:immunology*; D015342:DNA Probes; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D009993:Osmium Tetroxide / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013936:Thymidine / Q000737:chemistry / Q000276:immunology
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D015342:DNA Probes; D015082:2,2'-Dipyridyl; D004247:DNA; D009993:Osmium Tetroxide; D013936:Thymidine