Triggering effects of opsonized-IgG antibody on the superoxide release in the phagosome and phagosome-lysosome fusion by pulmonary alveolar macrophages in rabbits.
We studied the triggering effects of opsonized-IgG antibody on the superoxide (O2-) release and phagosome-lysosome fusion in the phagosome of pulmonary alveolar macrophages (PAM). Both were histochemically assayed in the same cell to enable simultaneous observation, using the qualitative superoxide dismutase (SOD)-inhibitable nitroblue tetrazolium (NBT) reduction test and the acridine orange (AO) method. When PAM from normal rabbit lungs were made to phagocytize the IgG-treated yeasts, the cells showed the enhanced release of O2- which acted directly on the antibody-coated yeasts as an oxidant, and the enhanced phagosome-lysosome fusion that was inhibited by SOD and was closely related to the enhanced release of O2- in the phagosomes. In contrast, neither enhanced O2- release nor phagosome-lysosome fusion was observed in the PAM which phagocytized saline-treated yeasts. These results may suggest that the opsonized-IgG antibody enhances the O2- release in the phagosome, resulting in the enhanced phagosome-lysosome fusion.
Suga
M
M
Kumamoto Rosai Hospital, Japan.
Ando
M
M
Tanaka
F
F
Araki
S
S
D000818:Animals; D007074:Immunoglobulin G; D066298:In Vitro Techniques; D016676:Macrophages, Alveolar / Q000276:immunology* / Q000378:metabolism*; D008561:Membrane Fusion / Q000276:immunology; D009895:Opsonin Proteins; D010587:Phagocytosis; D010588:Phagosomes / Q000276:immunology / Q000378:metabolism; D011817:Rabbits; D013481:Superoxides / Q000378:metabolism*; D014250:Trichosporon / Q000276:immunology
D007074:Immunoglobulin G; D009895:Opsonin Proteins; D013481:Superoxides