Correlation between DNA quantities in agarose gel and chromatographic areas of photographed DNA bands.
To quantitate DNA from photographs, correlations between chromato-scanned (in the fluorescence mode) and virtual areas from white DNA bands on photographs were examined. A linear correlation between the two measurements with coefficients higher than 0.89 was observed. The dynamic range of the fluorescence detection yielded good linearity for a wide range of amounts of DNA (microgram-ng) on the photograph.
Chinami
M
M
Department of Virology, Kurume University School of Medicine, Japan.
Tanikawa
E
E
Hachisuka
H
H
Sasai
Y
Y
Shingu
M
M
D002845:Chromatography; D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D010781:Photography
D004247:DNA