Potentiation of the antiviral activity of poly r(A-U) by xanthene dyes.
Ten xanthene dyes (XAN) are evaluated for their ability to potentiate the antiviral activity of poly r(A-U) using a human foreskin fibroblast-vesicular stomatitis virus bioassay in which the XAN is combined with 0.2 mM poly r(A-U) to produce a XAN/ribonucleotide ratio of 1/4. Four of the ten XANs tested in this study, rhodamine 123, rhodamine B, rhodamine 6G and sulforhodamine B, enhance the antiviral activity of poly r(A-U) 8- to 15-fold. The interferon-inducing activity of the four active XAN/poly r(A-U) combinations is equal to the sum of the activities of their constituents. These four XANs appear to potentiate the antiviral activity of the poly r(A-U) without superinduction of interferon. The direct viral inactivation study demonstrates that the XANs, poly r(A-U) and the XAN/poly r(A-U) combinations do not inactivate the VSV at concentrations near the 50% effective dose.
Jamison
J M
JM
Department of Chemistry, Kent State University, Ohio 44242.
Krabill
K
K
Hatwalkar
A
A
Jamison
E
E
Tsai
C C
CC
D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004396:Coloring Agents / Q000494:pharmacology*; D004357:Drug Synergism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology*; D008297:Male; D015394:Molecular Structure; D011063:Poly A-U / Q000494:pharmacology*; D012235:Rhodamines / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects; D014966:Xanthenes / Q000494:pharmacology*
D000998:Antiviral Agents; D004396:Coloring Agents; D007369:Interferon Inducers; D012235:Rhodamines; D014966:Xanthenes; D011063:Poly A-U