Linkage analysis of the human HMG14 gene on chromosome 21 using a GT dinucleotide repeat as polymorphic marker.
A (GT)n repeat in intron 4 of the functional human HMG14 gene on chromosome 21 was used as polymorphic marker to map this gene relative to the genetic linkage map of human chromosome 21. Variation in the length of the (GT)n repeat was detected by electrophoresis on polyacrylamide gels of DNA amplified by the polymerase chain reaction using primers flanking the repeat. The observed heterozygosity of this polymorphism in 40 CEPH families was 58% with six different alleles. Linkage analysis localized the HMG14 gene close to the ETS2 gene and locus D21S3 in chromosomal band 21q22.3.
Petersen
M B
MB
Center for Medical Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Economou
E P
EP
Slaugenhaupt
S A
SA
Chakravarti
A
A
Antonarakis
S E
SE
D002891:Chromosomes, Human, Pair 21; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005796:Genes; D008040:Genetic Linkage; D005819:Genetic Markers; D006609:High Mobility Group Proteins / Q000235:genetics*; D006801:Humans; D007438:Introns; D008297:Male; D010375:Pedigree; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid
D005819:Genetic Markers; D006609:High Mobility Group Proteins