A glucagon-secretin-like peptide stimulates the intrinsic nervous plexus of guinea pig gallbladder.
The role of vasoactive intestinal peptide (VIP), as a possible neurotransmitter of the intrinsic nerve plexus in the guinea pig gallbladder, was investigated by monitoring spontaneous contractile activity. VIP receptor antagonist (4 Cl-D-Phe6, Leu 17)-VIP did not produce any effect on muscular tone and spontaneous activity, whereas (N-Ac-Tyr1, D-Phe2)-GRF-(1-29)-NH2, (14-GRF analog), which is known to stimulate digestive enzyme secretion by interacting with the VIP-preferring receptors, greatly increased the amplitude and frequency of waves as well as the muscular tone. Since VIP receptor antagonist acts selectively as a competitive antagonist for the action of VIP, we conclude that the gallbladder inhibitory intrinsic plexus neurotransmitter is not VIP, but a member of the glucagon-secretin family of peptides.
Greco
A V
AV
Istituto di Clinica Medica, Università Cattolica, Roma, Italy.
Mancinelli
R
R
Mingrone
G
G
Racanicchi
C
C
D000818:Animals; D005260:Female; D005704:Gallbladder / Q000187:drug effects / Q000294:innervation* / Q000502:physiology; D005934:Glucagon / Q000502:physiology*; D004763:Glucagon-Like Peptides; D013007:Growth Hormone-Releasing Hormone / Q000031:analogs & derivatives / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000294:innervation / Q000502:physiology; D009433:Neural Inhibition / Q000187:drug effects; D018377:Neurotransmitter Agents / Q000502:physiology*; D010455:Peptides / Q000502:physiology*; D011964:Receptors, Gastrointestinal Hormone / Q000037:antagonists & inhibitors; D018005:Receptors, Vasoactive Intestinal Peptide; D012633:Secretin / Q000502:physiology*; D017337:Sermorelin / Q000031:analogs & derivatives; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology / Q000502:physiology
D018377:Neurotransmitter Agents; D010455:Peptides; D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; C048507:vasoactive intestinal peptide, 4-chloro-Phe(6)-Leu(17)-; D012633:Secretin; D014660:Vasoactive Intestinal Peptide; D004763:Glucagon-Like Peptides; C015236:glucagon-like-immunoreactivity; D017337:Sermorelin; D005934:Glucagon; D013007:Growth Hormone-Releasing Hormone; C045616:somatotropin releasing hormone (1-29)-amide, N-Ac-Tyr(1)-Phe(2)-