title: In vitro expression of bovine leukemia virus in isolated B-lymphocytes of cattle and sheep.
abstract: The purpose of this study was to determine the effect of T-lymphocytes and phytohemagglutinin (PHA), a T-cell mitogen, on the expression of bovine leukemia virus (BLV) in cultured B-lymphocytes from BLV-infected cattle and sheep. Bovine B-lymphocytes were isolated by negative selection via complement-mediated lysis of T-lymphocytes. Additionally, bovine and ovine B-lymphocytes were positively selected using fluorescence activated cell sorting. Expression of BLV in cultured bovine and ovine B-lymphocytes occurred in the absence of T-lymphocytes and without PHA stimulation. The results of this study demonstrate that BLV replication in cultured B-lymphocytes is T-cell independent. This finding may have implications for the mechanism of viral latency within infected B-lymphocytes.
authors.affiliation: Department of Pathology, Colorado State University, Fort Collins 80523.
authors.forename: W A
authors.initials: WA
authors.lastname: Jensen
authors.forename: S E
authors.initials: SE
authors.lastname: Sheehy
authors.forename: M H
authors.initials: MH
authors.lastname: Fox
authors.forename: W C
authors.initials: WC
authors.lastname: Davis
authors.forename: G L
authors.initials: GL
authors.lastname: Cockerell
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000382:microbiology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007938:Leukemia / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D001909:Leukemia Virus, Bovine / Q000187:drug effects / Q000254:growth & development*; D010835:Phytohemagglutinins / Q000494:pharmacology; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000382:microbiology; D014779:Virus Replication / Q000187:drug effects / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D010835:Phytohemagglutinins