The protective effects of allopurinol and superoxide dismutase-polyethylene glycol on ischemic and reperfusion-induced cochlear damage.
The purpose of this study was to assess the protective effects of allopurinol, a blocker of free oxygen radical (FOR) formation, and superoxide dismutase-polyethylene glycol (SOD-PEG), a scavenger of FORs, on ischemic and reperfusion-induced cochlear damage. Fifteen Wistar Kyoto rats (WKY) were randomly assigned to three groups: (1) a control group (5 animals) that was exposed to 15 minutes of cochlear ischemia by clamping the anterior inferior cerebellar artery (AICA), followed by 15 minutes of reperfusion as documented by laser Doppler flowmetry; (2) a drug-treated group (5 animals) that received allopurinol before ischemia/reperfusion; and (3) a drug-treated group (5 animals) that received SOD-PEG before ischemia/reperfusion. In the control group, the tone burst-evoked compound action potential (CAP) recorded from the round window (RW) of the cochlea was abolished, and the cochlear microphonic (CM) was reduced after ischemia. In contrast, both allopurinol and SOD-PEG-treated animals showed post-reperfusion sensitivity in CAP and CM measures. We interpret these results to indicate that damage to the cochlear from ischemia and subsequent reperfusion can be attenuated by pretreatment with allopurinol or SOD-PEG. This provides indirect evidence that FORs may be partially responsible for cochlear damage resulting from ischemic conditions.
Seidman
M D
MD
Department of Otolaryngology-Head and Neck Surgery, Henry Ford Hospital, Detroit, MI 48202.
Quirk
W S
WS
Nuttall
A L
AL
Schweitzer
V G
VG
D000200:Action Potentials; D000493:Allopurinol / Q000627:therapeutic use*; D000818:Animals; D001309:Auditory Threshold; D003051:Cochlea / Q000098:blood supply* / Q000503:physiopathology; D007511:Ischemia / Q000503:physiopathology / Q000517:prevention & control*; D011092:Polyethylene Glycols / Q000008:administration & dosage; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000627:therapeutic use*
D011092:Polyethylene Glycols; D000493:Allopurinol; D013482:Superoxide Dismutase