Stratum corneum lipid removal by surfactants: relation to in vivo irritation.
The relationship between the in vivo irritation potential of sodium lauryl sulfate (SLS) and linear alkyl benzene sulfonate (LAS) and the ability of these two surfactants to remove lipid from the stratum corneum (SC) in vitro were investigated. Either surfactant removes detectable levels of lipids only above its critical micelle concentration (CMC). At high concentrations the surfactants removed only very small amounts of cholesterol, free fatty acid, the esters of those materials, and possibly squalene. SLS and LAS have been shown, below the CMC, to bind to and irritate the SC. Thus, clinical irritation provoked by SLS or LAS is unlikely to be directly linked with extraction of SC lipid. The milder forms of irritation--dryness, tightness, roughness--may involve both surfactant binding to and denaturation of keratin as well as disruption of lipid. Our findings challenge earlier assumptions that surfactants' degreasing of the SC is involved in the induction of erythema.
Froebe
C L
CL
Colgate-Palmolive Co., Piscataway, N.J.
Simion
F A
FA
Rhein
L D
LD
Cagan
R H
RH
Kligman
A
A
D000476:Alkanesulfonates / Q000378:metabolism / Q000494:pharmacology*; D017738:Alkanesulfonic Acids; D002784:Cholesterol / Q000032:analysis; D004817:Epidermis / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D004890:Erythema / Q000139:chemically induced; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D006801:Humans; D007509:Irritants / Q000378:metabolism / Q000494:pharmacology*; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate / Q000378:metabolism / Q000494:pharmacology*; D012995:Solubility
D000476:Alkanesulfonates; D017738:Alkanesulfonic Acids; D005230:Fatty Acids, Nonesterified; D007509:Irritants; D008055:Lipids; C030641:alkylbenzyl sulfonic acid; D012967:Sodium Dodecyl Sulfate; D002784:Cholesterol