[In situ hybridization study of human papillomavirus from the penile cancer].
The histochemical detection of human papillomavirus (HPV) was performed using the technique of in situ hybridization from the formalin fixed paraffin embedded specimens of penile carcinoma and condylomata+ acuminata . One of 19 penile cancer cases (5.3%) revealed the positive staining on the nuclei of the tumors for HPV type 16/18. However, the positive nuclei were scattered and localized in small part of the tumor which showed no koilocytosis. On the contrary, 11 of 12 condyloma acuminata cases (91.7%) showed positive staining on the nuclei of the tumors for HPV type 6/11, also 5 of the 11 cases (45.5%) revealed cross reaction for other type of HPV. The HPV types 6/11, 16/18 and 31/33/35 may be unrelated to the pathogenesis of the Japanese penile carcinoma. However, further study is necessary to evaluate that the number of HPV-DNA copy in our cases is too small, or the other type of HPV is responsibility.
Moriyama
N
N
Department of Urology, Branch Hospital, Faculty of Medicine, University of Tokyo.
Nagase
Y
Y
Ueki
T
T
Hosaka
Y
Y
Higashihara
E
E
Murahashi
I
I
Aso
Y
Y
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003218:Condylomata Acuminata / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000302:isolation & purification* / Q000472:pathogenicity; D010412:Penile Neoplasms / Q000382:microbiology*; D014412:Tumor Virus Infections / Q000382:microbiology*
D004279:DNA, Viral