A simple, sensitive assay for the spermicide nonoxynol-9 in biological fluids by high-performance liquid chromatography.
A new high-performance liquid chromatographic technique has been developed to quantitate nonoxynol-9 in serum, urine, and vaginal fluid. The method is rapid, involves minimal sample preparation, and can be used to analyze a large number of biological fluid samples. The assay elutes a single nonoxynol-9 peak with no interfering components. This was accomplished using a 10-microns pelicular packed amine column, a normal-phase solvent system, and fluorescence detection. Nonoxynol-9 levels as low as 0.23 micrograms/mL in urine can be detected.
Beck
G J
GJ
VLI Corporation, Irvine, CA 92714.
Kossak
D
D
Saxena
S J
SJ
D001826:Body Fluids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D017137:Nonoxynol; D011092:Polyethylene Glycols / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013089:Spermatocidal Agents / Q000032:analysis*; D014621:Vagina / Q000737:chemistry
D013089:Spermatocidal Agents; D017137:Nonoxynol; D011092:Polyethylene Glycols