[Fundamental studies on differentiation inducers using a human embryonal carcinoma model].
A human embryonal carcinoma (EC) cell line, NEC14, was pluripotent and the tumors formed in nude mice contained differentiated somatic and extra-embryonic elements in addition to EC. The NEC14 cells could be induced to differentiate with 5 differentiation inducers in vitro, and N,N'-hexamethylene bisacetamide (HMBA) showed the most potent differentiator induction among them. All of the NEC14 cells were morphologically differentiated when exposed to 10(-2) M HMBA for 3 days. The differentiated derivatives ceased to proliferate in vitro and became non-tumorigenic in nude mouse. Several differentiation markers appeared in the differentiated derivatives. When the tumor-bearing nude mice were injected intraperitoneally with HMBA (10mg, twice a week), the tumor growth was inhibited. These results show that human EC is a useful model of differentiation-induction in cancer therapy.
Yamazawa
K
K
Department of Obstetrics and Gynecology, Chiba University School of Medicine.
Sekiya
S
S
Kimura
H
H
Shirasawa
H
H
Kawata
M
M
Takamizawa
H
H
D000081:Acetamides / Q000494:pharmacology*; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008954:Models, Biological; D009368:Neoplasm Transplantation; D013724:Teratoma / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000081:Acetamides; D004273:DNA, Neoplasm; C014026:hexamethylene bisacetamide