Characterization of complementary DNA encoding the precursor for gonadotropin-releasing hormone and its associated peptide from a teleost fish.
Reproductive maturity among male African cichlids Haplochromis burtoni is cued by a series of environmental and social interactions and is mediated physiologically by GnRH. A cDNA clone encoding the precursor for GnRH was isolated from this teleost. The molecular architecture of the predicted prohormone is analogous to that of the previously characterized mammalian forms; however, the predicted sequence of the associated peptide is strikingly different. Attempts to isolate a putative second precursor using low stringency hybridization were not successful despite evidence that a second related decapeptide exists in at least some teleost species.
Bond
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CT
Vollum Institute, Oregon Health Sciences University, Portland 97201.
Francis
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RC
Fernald
R D
RD
Adelman
J P
JP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010471:Perches / Q000235:genetics*; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D011498:Protein Precursors; D007987:Gonadotropin-Releasing Hormone; D004247:DNA