Analysis of immunoglobulin isotype blood levels, splenic B-cell phenotypes, and spleen cell immunoglobulin gene expression in mice infected with lactate dehydrogenase-elevating virus.
B-lymphocyte activation was studied in mice infected with lactate dehydrogenase-elevating virus (LDV). ELISA determinations of blood total immunoglobulin levels demonstrated that, at 10 days post-infection (p.i.) with LDV, only the IgG2a isotype was elevated. DNA-excess dot-blot hybridization showed that RNA specific for IgG2a and IgA immunoglobulin isotypes was increased in the spleens of mice at 10 days p.i. with LDV. Immunoglobulin surface phenotype analysis of spleen cells at 8-10 days p.i. with LDV revealed that there was no alteration in immunoglobulin isotype-bearing cell proportions, although total spleen mass and number of cells increased during LDV infection. When blood immunoglobulins from LDV-infected mice were analyzed by two-dimensional isoelectric focusing gels, followed by specific immunoblotting for immunoglobulin isotype, the presence of new IgG2a species was observed at 10 days p.i.
Hovinen
D E
DE
Department of Microbiology, School of Medicine, University of South Dakota, Vermillion.
Bradley
D S
DS
Cafruny
W A
WA
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D015153:Blotting, Western; D003520:Cyclophosphamide / Q000627:therapeutic use; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005803:Genes, Immunoglobulin / Q000502:physiology*; D006942:Hypergammaglobulinemia / Q000188:drug therapy; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis*; D007525:Isoelectric Focusing; D007772:Lactate dehydrogenase-elevating virus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D011061:Poly A / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D013154:Spleen / Q000276:immunology; D013997:Time Factors; D014777:Virus Diseases / Q000276:immunology*
D007132:Immunoglobulin Isotypes; D012333:RNA, Messenger; D011061:Poly A; D003520:Cyclophosphamide